LOCUS       DAZKNH010000001        76000 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000001 DAZKNH010000000
VERSION     DAZKNH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.001"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..635
                     /locus_tag="ACG8MG_000001"
     CDS             3..635
                     /locus_tag="ACG8MG_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374083.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(769..894)
                     /locus_tag="ACG8MG_000002"
     CDS             complement(769..894)
                     /locus_tag="ACG8MG_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFQ0374084.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1028..1921)
                     /locus_tag="ACG8MG_000003"
     CDS             complement(1028..1921)
                     /locus_tag="ACG8MG_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFQ0374085.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2103..3029)
                     /locus_tag="ACG8MG_000004"
     CDS             complement(2103..3029)
                     /locus_tag="ACG8MG_000004"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFQ0374086.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3114..3875)
                     /gene="pflA"
                     /locus_tag="ACG8MG_000005"
     CDS             complement(3114..3875)
                     /gene="pflA"
                     /locus_tag="ACG8MG_000005"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFQ0374087.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4119..6350)
                     /gene="pflB"
                     /locus_tag="ACG8MG_000006"
     CDS             complement(4119..6350)
                     /gene="pflB"
                     /locus_tag="ACG8MG_000006"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFQ0374088.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6627..9077)
                     /gene="parC"
                     /locus_tag="ACG8MG_000007"
     CDS             complement(6627..9077)
                     /gene="parC"
                     /locus_tag="ACG8MG_000007"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFQ0374089.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9089..11134)
                     /gene="parE"
                     /locus_tag="ACG8MG_000008"
     CDS             complement(9089..11134)
                     /gene="parE"
                     /locus_tag="ACG8MG_000008"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFQ0374090.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11300..11734)
                     /locus_tag="ACG8MG_000009"
     CDS             complement(11300..11734)
                     /locus_tag="ACG8MG_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFQ0374091.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11861..12499
                     /gene="plsY"
                     /locus_tag="ACG8MG_000010"
     CDS             11861..12499
                     /gene="plsY"
                     /locus_tag="ACG8MG_000010"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFQ0374092.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12631..13506)
                     /locus_tag="ACG8MG_000011"
     CDS             complement(12631..13506)
                     /locus_tag="ACG8MG_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFQ0374093.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13588..14379)
                     /gene="codY"
                     /locus_tag="ACG8MG_000012"
     CDS             complement(13588..14379)
                     /gene="codY"
                     /locus_tag="ACG8MG_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFQ0374094.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14397..15797)
                     /gene="hslU"
                     /locus_tag="ACG8MG_000013"
     CDS             complement(14397..15797)
                     /gene="hslU"
                     /locus_tag="ACG8MG_000013"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFQ0374095.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15811..16359)
                     /gene="hslV"
                     /locus_tag="ACG8MG_000014"
     CDS             complement(15811..16359)
                     /gene="hslV"
                     /locus_tag="ACG8MG_000014"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFQ0374096.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16396..17301)
                     /gene="xerC"
                     /locus_tag="ACG8MG_000015"
     CDS             complement(16396..17301)
                     /gene="xerC"
                     /locus_tag="ACG8MG_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFQ0374097.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17499..18875)
                     /gene="trmFO"
                     /locus_tag="ACG8MG_000016"
     CDS             complement(17499..18875)
                     /gene="trmFO"
                     /locus_tag="ACG8MG_000016"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFQ0374098.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18844..20922)
                     /gene="topA"
                     /locus_tag="ACG8MG_000017"
     CDS             complement(18844..20922)
                     /gene="topA"
                     /locus_tag="ACG8MG_000017"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFQ0374099.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21044..21901)
                     /gene="dprA"
                     /locus_tag="ACG8MG_000018"
     CDS             complement(21044..21901)
                     /gene="dprA"
                     /locus_tag="ACG8MG_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFQ0374100.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21957..22724)
                     /locus_tag="ACG8MG_000019"
     CDS             complement(21957..22724)
                     /locus_tag="ACG8MG_000019"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFQ0374101.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22717..23577)
                     /gene="ylqF"
                     /locus_tag="ACG8MG_000020"
     CDS             complement(22717..23577)
                     /gene="ylqF"
                     /locus_tag="ACG8MG_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFQ0374102.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23837..24235
                     /locus_tag="ACG8MG_000021"
     CDS             23837..24235
                     /locus_tag="ACG8MG_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0374103.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24472..25014
                     /locus_tag="ACG8MG_000022"
     CDS             24472..25014
                     /locus_tag="ACG8MG_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0374104.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(25097..25648)
                     /gene="lepB"
                     /locus_tag="ACG8MG_000023"
     CDS             complement(25097..25648)
                     /gene="lepB"
                     /locus_tag="ACG8MG_000023"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0374105.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25800..26027)
                     /locus_tag="ACG8MG_000024"
     CDS             complement(25800..26027)
                     /locus_tag="ACG8MG_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFQ0374106.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(26024..26542)
                     /gene="msrA"
                     /locus_tag="ACG8MG_000025"
     CDS             complement(26024..26542)
                     /gene="msrA"
                     /locus_tag="ACG8MG_000025"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0374107.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26562..27275)
                     /locus_tag="ACG8MG_000026"
     CDS             complement(26562..27275)
                     /locus_tag="ACG8MG_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFQ0374108.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27278..28174)
                     /locus_tag="ACG8MG_000027"
     CDS             complement(27278..28174)
                     /locus_tag="ACG8MG_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0374109.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28332..29174)
                     /locus_tag="ACG8MG_000028"
     CDS             complement(28332..29174)
                     /locus_tag="ACG8MG_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0374110.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            29306..29959
                     /locus_tag="ACG8MG_000029"
     CDS             29306..29959
                     /locus_tag="ACG8MG_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFQ0374111.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(30022..30549)
                     /locus_tag="ACG8MG_000030"
     CDS             complement(30022..30549)
                     /locus_tag="ACG8MG_000030"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0374112.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(30569..31516)
                     /locus_tag="ACG8MG_000031"
     CDS             complement(30569..31516)
                     /locus_tag="ACG8MG_000031"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFQ0374113.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(31528..33411)
                     /locus_tag="ACG8MG_000032"
     CDS             complement(31528..33411)
                     /locus_tag="ACG8MG_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0374114.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(33574..35652)
                     /locus_tag="ACG8MG_000033"
     CDS             complement(33574..35652)
                     /locus_tag="ACG8MG_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0374115.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(35665..35940)
                     /locus_tag="ACG8MG_000034"
     CDS             complement(35665..35940)
                     /locus_tag="ACG8MG_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374116.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(36302..36757)
                     /locus_tag="ACG8MG_000035"
     CDS             complement(36302..36757)
                     /locus_tag="ACG8MG_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374117.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(36911..38119)
                     /locus_tag="ACG8MG_000036"
     CDS             complement(36911..38119)
                     /locus_tag="ACG8MG_000036"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFQ0374118.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(38231..38563)
                     /locus_tag="ACG8MG_000037"
     CDS             complement(38231..38563)
                     /locus_tag="ACG8MG_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0374119.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            38711..39586
                     /locus_tag="ACG8MG_000038"
     CDS             38711..39586
                     /locus_tag="ACG8MG_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0374120.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(39668..40249)
                     /locus_tag="ACG8MG_000039"
     CDS             complement(39668..40249)
                     /locus_tag="ACG8MG_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFQ0374121.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(40255..41514)
                     /locus_tag="ACG8MG_000040"
     CDS             complement(40255..41514)
                     /locus_tag="ACG8MG_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0374122.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(41516..42562)
                     /locus_tag="ACG8MG_000041"
     CDS             complement(41516..42562)
                     /locus_tag="ACG8MG_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374123.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(42797..43072)
                     /locus_tag="ACG8MG_000042"
     CDS             complement(42797..43072)
                     /locus_tag="ACG8MG_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFQ0374124.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(43315..44625)
                     /gene="der"
                     /locus_tag="ACG8MG_000043"
     CDS             complement(43315..44625)
                     /gene="der"
                     /locus_tag="ACG8MG_000043"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFQ0374125.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(44807..46036)
                     /gene="rpsA"
                     /locus_tag="ACG8MG_000044"
     CDS             complement(44807..46036)
                     /gene="rpsA"
                     /locus_tag="ACG8MG_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFQ0374126.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(46171..46851)
                     /gene="cmk"
                     /locus_tag="ACG8MG_000045"
     CDS             complement(46171..46851)
                     /gene="cmk"
                     /locus_tag="ACG8MG_000045"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFQ0374127.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(46900..47535)
                     /locus_tag="ACG8MG_000046"
     CDS             complement(46900..47535)
                     /locus_tag="ACG8MG_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFQ0374128.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(47601..49004)
                     /locus_tag="ACG8MG_000047"
     CDS             complement(47601..49004)
                     /locus_tag="ACG8MG_000047"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFQ0374129.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(48991..50022)
                     /locus_tag="ACG8MG_000048"
     CDS             complement(48991..50022)
                     /locus_tag="ACG8MG_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0374130.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            50053..50274
                     /locus_tag="ACG8MG_000049"
     CDS             50053..50274
                     /locus_tag="ACG8MG_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFQ0374131.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(50439..51176)
                     /locus_tag="ACG8MG_000050"
     CDS             complement(50439..51176)
                     /locus_tag="ACG8MG_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFQ0374132.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(51329..52669)
                     /locus_tag="ACG8MG_000051"
     CDS             complement(51329..52669)
                     /locus_tag="ACG8MG_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0374133.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(52906..54429)
                     /locus_tag="ACG8MG_000052"
     CDS             complement(52906..54429)
                     /locus_tag="ACG8MG_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0374134.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(54807..55418)
                     /locus_tag="ACG8MG_000053"
     CDS             complement(54807..55418)
                     /locus_tag="ACG8MG_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0374135.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(55541..55666)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(55748..56464)
                     /locus_tag="ACG8MG_000054"
     CDS             complement(55748..56464)
                     /locus_tag="ACG8MG_000054"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0374136.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(56470..57039)
                     /gene="scpB"
                     /locus_tag="ACG8MG_000055"
     CDS             complement(56470..57039)
                     /gene="scpB"
                     /locus_tag="ACG8MG_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFQ0374137.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(57023..57793)
                     /locus_tag="ACG8MG_000056"
     CDS             complement(57023..57793)
                     /locus_tag="ACG8MG_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFQ0374138.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(57790..58161)
                     /locus_tag="ACG8MG_000057"
     CDS             complement(57790..58161)
                     /locus_tag="ACG8MG_000057"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0374139.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(58180..59067)
                     /gene="xerD"
                     /locus_tag="ACG8MG_000058"
     CDS             complement(58180..59067)
                     /gene="xerD"
                     /locus_tag="ACG8MG_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFQ0374140.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(59163..59636)
                     /locus_tag="ACG8MG_000059"
     CDS             complement(59163..59636)
                     /locus_tag="ACG8MG_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0374141.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(59741..60604)
                     /locus_tag="ACG8MG_000060"
     CDS             complement(59741..60604)
                     /locus_tag="ACG8MG_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFQ0374142.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(60800..62899)
                     /locus_tag="ACG8MG_000061"
     CDS             complement(60800..62899)
                     /locus_tag="ACG8MG_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFQ0374143.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(63119..64228)
                     /gene="rpoD"
                     /locus_tag="ACG8MG_000062"
     CDS             complement(63119..64228)
                     /gene="rpoD"
                     /locus_tag="ACG8MG_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFQ0374144.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(64252..66093)
                     /gene="dnaG"
                     /locus_tag="ACG8MG_000063"
     CDS             complement(64252..66093)
                     /gene="dnaG"
                     /locus_tag="ACG8MG_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFQ0374145.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(66275..68590)
                     /locus_tag="ACG8MG_000064"
     CDS             complement(66275..68590)
                     /locus_tag="ACG8MG_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0374146.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(68775..69635)
                     /locus_tag="ACG8MG_000065"
     CDS             complement(68775..69635)
                     /locus_tag="ACG8MG_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFQ0374147.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(69694..70326)
                     /locus_tag="ACG8MG_000066"
                     /pseudo
     CDS             complement(69694..70326)
                     /locus_tag="ACG8MG_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            70493..71254
                     /locus_tag="ACG8MG_000067"
     CDS             70493..71254
                     /locus_tag="ACG8MG_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0374148.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            71280..73871
                     /gene="mprF"
                     /locus_tag="ACG8MG_000068"
     CDS             71280..73871
                     /gene="mprF"
                     /locus_tag="ACG8MG_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0374149.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(74059..75246)
                     /gene="tuf"
                     /locus_tag="ACG8MG_000069"
     CDS             complement(74059..75246)
                     /gene="tuf"
                     /locus_tag="ACG8MG_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0374150.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            75614..75970
                     /locus_tag="ACG8MG_000070"
     CDS             75614..75970
                     /locus_tag="ACG8MG_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0374151.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT        20745 a        16020 c        13048 g        26187 t
ORIGIN      
        1 ttatggaaat tcaacgtaga accactaatt ttttacattt aggctctttt tttgcaactt
       61 tgacgatctt attttttcta tgttattcat accttaagac ctcacatgcg ccttctagtg
      121 cattaggctg gttgcttttg gatcattctg atatcacaga agccgaagaa aatccgtttt
      181 ttttgatttt gtcttctctt tgcggcttac tgttttctgt ttatttcggt gcctattttt
      241 ttcatcgaca tcctggtacg ttaaacgaag cttcaagcgt attgattgca tttaaaacgt
      301 tttctttttt agcaattctt ttttattcgt ttaatcacaa tacgcttttc ttctttatca
      361 tgaatggttt agtcgtgatt ttatccattt ttacttattt tattttttca ctcattttta
      421 gccaattgaa atgtccgctc ttctttcgat tagttcctgt cacctgtttt atttacagct
      481 tgttttcctt ttttgtcctt gctttaatgc cactgaccaa cccttcgacg atccaagtcg
      541 caggaaatct gttggatatg ctcttcatcg tcagtctagg tgtatttatg tggcaaagaa
      601 aaaacaaaca aaataaaaag cattttgaag cctgactatt acgacttcat aaatgctttt
      661 ttagttttaa acacttcaat ctaaaagaat aacccaagat tggataaatt attaatgact
      721 cgccattcgt ttcttagtga ttctggatta tcaaatggac ttcctaatct aatcagctag
      781 ttcttcccat aaaatgccat acttgactga atcataatat tgattattta aaaatctaac
      841 ttttcttatc actacttcct ttgtcattcc acatttctca ccattttttt gcattccttt
      901 gtttccagac catgtagtga atctaatagg tggtagatag gcaaaatact caaataattc
      961 ttttaaccat aaaggaagag aaaggctcct cctgcaagag tcagtataat ttttgcttac
     1021 tgctctctta ggttttactc actaaaactg ttccatattc tttgatataa gaaaatgcat
     1081 gcggtataaa tttcttgctc agctcagaaa agatttgtcg tttaagaaag gctatttcct
     1141 ccattgtcaa tgaaggatcc aatgttggta attcaatctc aaaattgttt ttttcgacaa
     1201 taggtaattc taatgtgaat gattcttctt cctttatagt attcgtcaca agtagagact
     1261 gatggaaaat actaggccgg cgatttttat atttattctt acgtatcctg tcaacgataa
     1321 cttctatttg atcaaaaaag tacgattcat ttacgtctcc aataagttcg gctaattctt
     1381 tgaatgcctt cgttacagga agctgattct tctgtacaaa aaagtaatta gccaaagtaa
     1441 gcgggaagtg ttctatagag gaaagtgtga taaaacggta cttctcattt cctgcagttg
     1501 tcctcagcaa ttctgtctct cggctgtttc ttactccata aaaatacgct tctttccgtt
     1561 catcttcgaa acaatacgcc caaataactt ctcccatcgc taatttttgt tcatcaacag
     1621 agagtctctt caattgttca gctatggttt cagcagccga agtggcttgt tgcaagtaat
     1681 ctttaggatc aaatattgga aagttcaaat gatatcttcg ttcttccctc ttgattaatc
     1741 ctgcatcgat caataaatca agaaaatggg aaaatttatt ttgcgaaaat tcttttttca
     1801 attctcttag aatcgtttct tgttctcgat gttcatatag ataatcggtc aatgaagaaa
     1861 aaagcggatc atcggtcagt ttttgataaa agttttcctt attttttgaa taatagttca
     1921 tatatgttct cttttctatc cgttttattt tacttaaaag gcttttacgt aaaaaggagg
     1981 aagaacaaga gtcataattg actgttattc tcctccattt cttcggcctt tagtatgttc
     2041 tatgtttctt tttatatcaa tcgcttatcc attcattttg aatgcttcat actaccattt
     2101 gattagctaa atacagccgt caattgaggt acgacttgtt ttttacgtga aacgacacct
     2161 ttaagcaaag cacggttgtt ggtcaaagtt gtttggaaag cttcttctac tttttctttt
     2221 cctttaccag caacaagtaa ttctgaatca ctgtctaaga tgtttgtgac aactaatacg
     2281 aacagatcgt agccatttgc tttcatttca tcttccattg ctgcttctag ctctgcttgt
     2341 cgatccatca cttcattaag gtccactgta ttgacttgag cgatacgaac gttggaactg
     2401 cccattggga aactttttgc atccaaatca agaagaaccg cagctgattt atcacttaga
     2461 tttgttcctg ctttcaacat ttcaagtcca taagtgttta gatcgacttc agcgattgct
     2521 gcaagttctt ttgcagctgc ttcatcttca ggcgtacatg ttggtgattt gaacaacaat
     2581 gtatctgaaa tgatagctga gagcatgatt cctgcgattt ctttaggaat tgttacttgg
     2641 ttttctttat atagttttaa aataatcgta cttgtacatc caactggttc tgcacgatag
     2701 tacaatggat tagctgtttc aaagtttgca atacgatggt ggtctactac agctaaaata
     2761 tttacttcgc tgatatctga aatactttgt tggaattcat tatgatccac aagcatgaca
     2821 tctgcatttt ctgctttagc tgactctact actcgtggtg cagatagctg aaagtggtct
     2881 aatgcgtatt gtgtttcttc attaggtgta cctagagcaa ctgcttctgt ttctttacct
     2941 aattcatttt gcaagtgtga aaacgcaatg gctgccccga ttgcatctgt atctggattt
     3001 tggtgaccaa aaactaaaat ttttgacatg tcattcgctc ctttgtgatt tctcctattt
     3061 aataatagca aaaaaacgga aagaagcaat acttatcttc tttccgatgc gacctatcgc
     3121 gttaaatatt ttgtatactc ttctgtatga agcaatcgtt tagcattttc gacacgttct
     3181 tttgtaggtg gttcgatccc ttctaatgga tatttcaaac caagtgtttc ccacttgtat
     3241 tttcccatcg tatgataagg aagaatctct actctgtcta catttgacaa ggtttgaata
     3301 aagttgtcta gtcggatcaa atattcatca taatcacttc gctcaggaac tagtacatgg
     3361 cgaatccaaa ccggtttgtt gatttttgac aaatattgag ccatttctaa aatattttca
     3421 ttgtcccaat gcgttagttt tttatgctct tcattatcga tgtgtttgat gtcaaataac
     3481 agtaaatctg tatacttcat caattcttga aaacgtgaaa agaaaggttc ttcatacgta
     3541 aaaggttttc cgcatgtatc caatgtagta tgcatcttcc gctcttttgc ttttttaaat
     3601 agttcgatca aaaaatcgat ctgcaataga ggttctccac cactaacagt aataccacct
     3661 ttatctcccc agtaaggtct gaagcgttct gctttatcta aaagttcatc tgctgtgtat
     3721 tctttccctc cgccaatatt ccaagtatct ggattgtgac aaaattggca acgcatgcgg
     3781 catccttgca taaacacgac aaagcgcagt ccaggtccgt ctactgaacc gaaagtttcg
     3841 atagaatgaa catagccgat tgttttttct tccatttaag ttcacatctt ttctttgatt
     3901 tcattttaag tacaagttag ctgtcgaagc gaagctttct gaacaaaatt tttcgcaatt
     3961 gccgcttcgt tatgtgtaaa atttacaggg acggaacata caagtcctgc ccctgtaaat
     4021 cgtttctttc aaataagcat actgattatt cgattttgat gcgatggcta ttcaacgtcc
     4081 tttcgacgct aagtcgaaaa acttttttca cgaacttttt acattctgtc gtgtgaagtt
     4141 ctcatcaaga catctaattg ttgttctctt gtcaagtcac ggaatttaac tgcataacca
     4201 gatacccgga tcgtcaagtt cggatatttc attggatttt ccattgcatc acgcaataaa
     4261 tcattactga atacattgac attcaaatga taagctcctt ttgagaagta tccatctaag
     4321 acattacgca agttttcgat tcttgtatct tgttcttttc caagacctga aggattgatt
     4381 gtttgggtat ttgaaattcc atccaatgca cagccgtaat tcattttagc tgttgagttc
     4441 aggctagcta ataacccact ttgttccgca ttgtaacttg gatttgctcc tggcgcaagt
     4501 ggttttccag cttcacggcc atcaggagta tttcctgtat ttttaccgta aacaacattt
     4561 gaagtaatcg tcaataatga agttgttggt tttgagttgc ggtaagtttt atgacgtttg
     4621 atttgtccca tgaagtattc caagatccat gaaactaatt catctgcacg gtcatcatca
     4681 tttccgtatt tagggaagtc gccatccact tcaaaatcaa ctgctattcc tttttcatta
     4741 cggattgctt ttacttttgc atatttgatc gctgacaagc tgtctgccgc atgagagatc
     4801 ccagcaatac ctgtagcaaa tgtgcgttgc aagtcagaat ccatcaaagc cagctgagct
     4861 gcttcataag aatatttatc atgcatataa tgaatgatgt tcaacgtatt gacatatagt
     4921 tctgctaacc aatccatcat attcatgaag ttatgtttca cttcttcaaa gtctagtatt
     4981 tctgatgtga ttggacgata ttctggtcca acttgggcgc cagtcacttc atcgactccg
     5041 ccattgatgg catataatag tgttttagcc aagttagcac gagcgccaaa gaattgcatg
     5101 tcttttccga caactgttcc tgatacacag caagcaatcg cacaatcatc agaaccccat
     5161 tgttcacgaa gctgattatc attttcgaat tgaatcgaag aactttcgat agcgattctg
     5221 ctagcatatg ttctgaatcc ttctggaagg tgttcagaat acaaaacagt caagtttggt
     5281 tctggtgaag gtcccatatt tgttaatgta tgaagcagac ggaaatcatt ctttgtaact
     5341 aatgaacgac catctaatcc catacctgca atggataaag tagcccagat agggaatcct
     5401 gagaataatt ggttatattc aggtgtacgc gcgaatttga ccatacgtaa tttcattact
     5461 aagtggtcga tcaattcttg tgcttctttt tctgtgatta ctccattatc caaatcacgt
     5521 tggatataga tatcaaggaa tgctgagata cgtccgattg acatcgcagc accattttgt
     5581 gatttgattg ctgctaaata tccgaagtat agccattgga cagcttcttt tgcatttgca
     5641 gctggtttag aaatatcata gccatatttc gcagccattt ctttcatttg cgctaatgca
     5701 cgatattgtt cagtgatttc ttcacgtaaa cgaatcactt cttcactcat tgtatgataa
     5761 ccagtcatat catgatcttt tttcttttgt tccatcaaat agtcgattcc atacaatgcc
     5821 acacgacgat agtcaccgat gatacgtccg cgaccgtaag cgtctggaag ccctgtaatg
     5881 attttgtttt tacgagcagc acgcatttca gcagtataag cgtcaaatac accttgatta
     5941 tgtgttttgc gccagtcagt gaaaatatgt gtcaattcat cgtttggtgt atagccattg
     6001 ctttttagcg cattgttagc catattgatt ccgccgaacg gcatgaatgc ttgtttcaaa
     6061 ggtttgtctg tctgtagacc gacgattttt tccaaatctt tatccaaata gcctgcatcg
     6121 tgtgaagtaa tagttgatac gatttctgtg tccatatcat agacaccgtt acgctcgaat
     6181 tggatatcat ttaacttttg caattcatcc cataattttt ctgttgcttc tgttgggcct
     6241 tccaaaaagc tgtcatcacc gcgatattca gtgtagttat tttgaataaa gtctctagtg
     6301 tctacgcctt ctttccattt ggtaccttta aatccattcc attgctccat tgtatggacc
     6361 tcctaaattt ctgttttatg taacagtttt tacgtgatta gtataacaca gcaccctatc
     6421 tttgcaaggg ttttcttgtt ttttatttaa caatttttat gttttattta catatattta
     6481 aatcaacgtt gacataccaa catttaagca ttgtgaacac tttcatcttt ttttgcaaac
     6541 gtataaggat ttattaaaaa tgaatgaaaa agagacgcca cctgtattaa tacagatggc
     6601 gtctcttcat taaaatacta caacagttac gactcgatga aaatctcagg agcatcgata
     6661 acttcgatga cttctccacc ttttttctca tccatcgcaa atgatccatt cgatgttcga
     6721 tcacctatag ggaaatcagt tgggtcaatc gttaactgct gccctttttg attcatcaag
     6781 gttatttctt ttgatcttcc ttccgacata tagataatac ggtgcggatt ttttttcagc
     6841 tcacgcatga ccgtcactcc ccgcttcgca cgtgaagttt ggctgatttc ttgagcaagc
     6901 atgcgtttaa cagctcctcg ctgagtaacg attacgatcg gcgtatcacc ttctgctaac
     6961 acaagcagcc cgttgataat cctatctcca tctttcaaat tcatagactt cactccagct
     7021 gcctttgctc caacaacggg aacatctgtt aaaggatagc gcaaacccat tccttgatgc
     7081 gttactaaga aaacatctaa gcctggctga tcttctttca gatacacagc aatcagttca
     7141 tcttcttccg cctttagctt catacagctg agcggacggc tcttataggt gcgccatggt
     7201 tcgaactctg tcattcgtgt ttgcttgatc aatccattct tgctgatgaa tacaaatgtt
     7261 ttctctgctt gaattttttg atatgggaaa acagctaaaa tcgattcgtc tacagacaga
     7321 ttggagattg tttgagaaat gtgctcacca gcatctttcc atttcaaatc gggtagttca
     7381 tggacagggc ggtagatcac atttgcttta tttgtgatca gaagaacatg gtccaatgta
     7441 ctcaattcac ctgagtatag caagtagtcg ccttctcgca tcccgatttc ttctggtttg
     7501 gaagcactat aagaacgaag gcttgttcgc ttcacatagc cttctcgcgt cacactgaca
     7561 attacgtctt cttgtgccac taagacttgc gtatcaattt tgatttcttc gatttcgtct
     7621 tcgattgtcg ttagacgagc ggaagaataa ttttttttca cttctcgcaa ttcttttttc
     7681 ataacagaga aaagctcttt gtcatttgaa aggattttat tcaactctgt gatttgcgca
     7741 atcaagcttt cggattcctt ttgaagctct gtgatatccg tgttcgtcaa acggtataat
     7801 tgcaatgtga caattgcttc tgcttgttct tctgtaaatt gaaagacgtc gactaaattc
     7861 tttttggcgt cttttttgtc tttactttca cgaatcgttg cgattacttc atccagaatc
     7921 gataaagctt tcatcaatcc ttctacgata tgctgtcttt tttgagcttt ggctaaatcg
     7981 aattgactac gttttgttat gacttgcttt cgatgctcga tataacttct taaaatgtct
     8041 ttcagtccaa cttgatgcgg agtcatatgg tcgatagcga ccatattgaa attgtaattg
     8101 atctgtagct cagtgttttt gaacaaataa ttcaaaattc cttgagcatt ggcgtctttt
     8161 ttcaattcga cgacaatttg cagacctgtt ctatcacttt catctcgtac ttcagctatt
     8221 ccatcgattt tcttattcaa acggatctca tccatttttt tcaccaaggt cgctttattt
     8281 acttcatatg ggatttctgt gatcacgatt tgctgtttat tacccttcat tgcctctatc
     8341 tttgttttcg aacgaagaat aacttttcct cgtcctgttt cataagcttt tttgatttca
     8401 tcttttcctt gcaagatccc tcctgttgga aagtcaggtc caggaatata ttccatcaat
     8461 ttttcaaggc tcgcttgtgg atgatcgatc atatagattg tgccatcgat cacttcagct
     8521 aagttatgag tcgggatttc tgttgcatat ccagcagata tacctgtcga tccattgacg
     8581 agaaggtttg ggtatcgagc tggaaggact gttggttctt tttctgtatc atcaaaattc
     8641 cacacgagat cgactgtttc tttctcgata tctgctagca tctctccact taacttggat
     8701 aatctggctt cagtataacg catcgcagca ggtggatcac cgtccatact cccgttattc
     8761 ccgtgcattt cgatcagtac ttcccgcaac ttccagtctt ggctcatgcg gaccatcgct
     8821 tcataaatac tgatgtcacc atgaggatga taattcccca tgatatttcc gacagatttt
     8881 gctgatttac ggaatccttt gtcatacgta ttgccatctt tgttcatggc aaataatatc
     8941 cggcgttgca ctggcttcaa tccatcacgg atatcaggca gtgcacgttc ttggataata
     9001 tatttggaat aacgtccgaa gcggtcaccc atcacttctt ctaaagttaa ttcttgtact
     9061 tcttgtcgat tttccatcag gtgacctcct attctacttc aaataactga ttgttttcat
     9121 ttttatctgc tcgttcttca tctaataaat cattagaaac tgaaggagag atttcagtat
     9181 cctctttttt atctaagata ctgccatctt cttcgagcgt aaattgcacg tgattttcga
     9241 tccattttct tcgaggttct actttgtcac ccatcagtgt ggttactcga cgttcagctt
     9301 gcgcagcatc atcgatacgg acgcggatca acgtacggga agtcggatcc atcgttgttt
     9361 cccacaattg ttccgcattc atctctccaa gtcctttata tcgctgcagc atatatcctt
     9421 tgccgacttt tttaatcatt gctgccaatt cgtcatctgt ccacgcatat tcaatgactt
     9481 gcttctttcc ttgtcctttg gatactttat ataaaggcgg taacgcgata tatactttcc
     9541 cagcttcaat cagcggtttc atataacgat aaaagaaagt aagcagtaat acttgaatat
     9601 gggcgccgtc tgtatccgca tcggtcatga tgattacttt gtcgtaatta cagtcttcga
     9661 tagaaaactc aggaccgact cctgcaccaa tcgtatagat catcgtattg atttcttcat
     9721 ttttcaagat atcttgcatt ttagcttttt cggtattgat gacttttcct cgtaaaggta
     9781 gaattgcttg gaactttctg tctcttcctt gtttagcaga tccgccagca gaatcccctt
     9841 cgactaaata caattcattt ttcttagggt ttcgggattg tgcgggtgtc aattttcctg
     9901 aaagaagaga ttcgcctttt ttacgcttct tcccatttcg gctttcttct ctggctttac
     9961 gtgctgcttc tctcgcttct ctggctttga ttgctttacg gaccaacatc tggctcattt
    10021 cgctattttc ctgcaaataa aagcccattt gttcactgat gacgttatct actacggtac
    10081 gagcaactgg cgtaccaagt ttttcttttg tttgcccttc aaattgcaga agattttcag
    10141 gtacacggat agaaagtaca gcagctaacc cttctctgaa atcactacct tccagattct
    10201 tgtcccgttc tttcagcaaa ccaacttttc tcgcatattc attataagat ttcgtcatcg
    10261 ccgctttcat accagcttca tgggttcccc catcttttgt ccgaacgtta ttgacaaaag
    10321 ataaaacatt ttctgaataa ccgtcattgt attgataagc gacctctact tcgatacctt
    10381 ctttttctcc tgaaaaataa acaactggtg tcaatgtatc tttttcttca ttcagataat
    10441 caacaaattc tttgattcct tcttcgtaat gaaaaatttc cttgactggt tcttctccac
    10501 gaagatcaga aagagagatt ttaacgcctt ttagaagaaa ggcagattct cttaaccgtt
    10561 cggctaaaat ttcatatgaa aatttcgttg tactgaatat tgtatcatct ggtaaaaaat
    10621 ggacagtcgt cccgtttgct ttttttgttt ttcctatctt tttcaaagta ccgtcaggtt
    10681 taccgccgtt tttaaatttc tgctgatact ctacgccatc tctaacgatc gttacaatta
    10741 gccatttcga taaggcattt accacactgg ctcctacacc gtgcagtcca ccagaagttt
    10801 tgtaccctcc ttgaccaaac tttccgccag catgcagcac agtaaaaatc acttcaactg
    10861 ttggtattcc ggaagcatgc attcctacag gcatcccacg tccgctatct gcaacagtta
    10921 tactattgtc ttcatggatg gtcacatcaa tctcactgcc ataacctgat aatgcttcgt
    10981 caactgcatt atcgacgatt tcatagacta aatgatgcaa gcctcggctg tcagtggatc
    11041 caatgtacat ccctggtctt tttctaactg cctctaatcc ttccaatact tggatggacg
    11101 agtcattgta ttcgttgttt actttttttg ccaaggcaaa cgctccttca ataatacttt
    11161 gcggcaaaac cgcaccacga ttatcatact agaaaaggtc gtgaaaaaaa agagggtaga
    11221 cgagaaatca attttaatta tctcctaacc gctttttcga cattcccctc tatcgatcat
    11281 ctcttctttg tggtcatttt tatttttggt tttctttcaa cttaccaagt tcaatcttaa
    11341 tacaacgatc cattacgata tctgtacgtc ctgcttttct tagcatctca gctgcttctt
    11401 tgttttcaat ccctaattgt gcccagaaca ctttagcatc agttttcaaa aaatcacctg
    11461 ctatttcagg taaaaattca ctccgtctaa atacatctac gatatcgatt ggatcatcga
    11521 tttctgtcaa attcgcatag actttctcgc ctagaatttc ttcaccagcc aataatggat
    11581 ttactggtat gattttatat ccattgtctt gcaatacctg tgcaaccatg taacttgttc
    11641 gttctggttt attgctcaag cctacgacgg caatattttt tgcttcttcc aaatagttga
    11701 aaatcgtttc ttgtgttgga ttttttacag acatttctgc caccccttgc ttttcttttt
    11761 attccatcta ttatacttgt atctttcatt ttttcaattg ttcctgttac ctattcatgc
    11821 taaaataaag gatagttttc aacttgaaag agggtaaaaa atgaaattga tcttattact
    11881 tattttagca tatcttttag gttctattcc ttccggtgtc tggattggca aattgttttt
    11941 caaaaaagat atccgacaac acgggagcgg taatactgga acgaccaata catttcgtgt
    12001 attaggcaaa acagccggga ttgttgtcct gcttatggat attttaaaag gtacattggc
    12061 tacttgtttg ccgatgatct tccacctttc tgggattaac cctctttggt ttggggtatg
    12121 cgccattctt ggccatacat ttccgatttt tgctgggttc aaaggaggaa aagctgttgc
    12181 gacaagtgca ggaatgcttt taggatttaa tcctgtattt tttgtttatt cttgtatcat
    12241 ctttatcgtt agtttatttt gtacaagtat ggtgagtttg agcagtatga tcagtgcagt
    12301 attgatcact ttatctacga tcgtccttcc ttatgtagct cctgtgattt tggctaagcc
    12361 caattggtta ctgacgatca ttgccttttt agtttcttcc tttatctttt atcggcataa
    12421 agacaatatc aagcgtatca gaaaccatac tgaaagtcga atcccgtttg gtttgaatag
    12481 cagcaagaaa aaatcctgat actttttcat tccaaagaga taaaacggga gtgtgacaaa
    12541 tcgatgtcac actcccttat tacttacagg atagcttttt tacacacctt ggtaaacggt
    12601 gttccctgct aacttctcgg tcacaacctc ttacttcact gtgatggaat attttgtctt
    12661 gaagatttca tgggcaccta gttgattgat ccctactttt tccatcaagt ttccactact
    12721 gttcaccgtg tcagcgattc cgcaccacgg ttcgatacaa acgaaaggcg cttcttttgg
    12781 ataaggagac cagatcccta cataaggcat atctttatag cttaaagtta cgctatggga
    12841 agatttttca cttcgaatgg cgtacgcatt tagacccctt gtttcaaaaa ccaaggcatc
    12901 ttgctcaaaa agttcacgat tcaaagccag attcgtattt gtctgaccta acgtcttttg
    12961 atccatatcg atgaatgggc ctttcaatgg taggcgtaat cttgatttca atggagaaaa
    13021 tgccagatag aaatcttcaa atgacaatcc ttcttcaaga ggtacattga aagcaggatg
    13081 tccaccgata gaaaagaaca tttctttttc accggtattt gccacttgat aatgaaccgt
    13141 gatgccgtct cctcctaatt catagctgat cactaattca aaatcaaatg gatacttttc
    13201 taatgtctcc ggcgtactcc ttaagatgaa actagccatt tcttgtccat gttcaatgac
    13261 ttcaaaatcc atatcccgag caaatccgtg ttgccccatt tgatatgttt tcccttgata
    13321 aacatactga tcatctttta aacgtccaac aaaagggaat aaaaccggtg catgtctgcc
    13381 ccaatacgct ggatcacctt gccagatata ctctaaacca tgttcttttg atttcagact
    13441 gatcaattcc gcaccgtgtt cagcgatttc ggcgatcagc tggtcatttt gaataataac
    13501 agtcattgcc attcctccaa cttcgactta ctttttaaaa tgggaaggct ggaacaaaca
    13561 tttctatctg ccccagtccc cttacttcta ataactgtgt tttgctaact cgtctttaaa
    13621 acgggaattc aatactttta aataagttcc cttcatgcct aacgaacgag attcaatgat
    13681 tccagctgat tccaattttc gtaaagcatt gacgatcaca gaacgagtga tgccgatact
    13741 atcggcaata ttagaagcag tcagacgacc ttcatctcca tccaatgctt caaagatagc
    13801 ttgtacagcc ttcaattcgc tataagataa tgtgttgatt gccatttgga cagccgttgc
    13861 actccgtaca tctgcttcga tgcttcgaga cttctgataa aggatctgca taccgacaac
    13921 cgtagctcca tattctgcta agactagatc gtcatctgca aacgattgtt ccatacgaga
    13981 taagataatc gtacccaaac gttcgccagc tccaaagatc ggaacgacag tcgtcagacc
    14041 aaatggatat ttctctcttg actctaccgg aaatgcagta atatcactag taattgggat
    14101 attagcttca gttttcatca actgtgccac tgtatcagta taagattcag gaaattgttt
    14161 ctcaacaaac attgttttga ttcgttcatt gttcacatcg tgacgttcat tataacctag
    14221 taatactccg tcacttccta tgatataggt gttgctgtca agaataccac ctagtgtgac
    14281 agccatttta tcgtaaggca aatctgcttg catgtcaaaa gtattctttt gctgcaaaag
    14341 tgtgtttatc ttgcgggttt tcgttagcaa gtctgtcatt gtcgtcctcc gattttttat
    14401 aaaatatagc gacttaaatc ttcatttgcc gcaattgagc caagtttgtc atccacatat
    14461 gcttctgtga tcgttatttc tcccatttgc atatctggtg cttcaaataa tagatcttct
    14521 agtagttttt ccaaaatggt atgcagtctg cgtgcgccga tattatctgt gtcacggttg
    14581 acatcgaaag cgatttgagc gatccgttca attgcttctt ttgtaaacgt aactgacaca
    14641 ttttctgtac cgatcaacgc gacgtattgt tttactaaag cattatttgg ttcggtcaga
    14701 atcttcacaa aatcttcagc tgtcaaatca tctaattcta cccgtatcgg gaagcgtcct
    14761 tgaagttctg gaattagatc acttggttta gacaaatgga aagctcctga agcaatgaat
    14821 aggacgtgat ctgtttggat gatcccgtat tttgtgttta cttgtgaacc ttcgacaatt
    14881 ggcaatatgt ctctttggac gccttctctg gatacttctc cagattgctg tgatttagaa
    14941 gtgatcttat cgatttcatc gataaaaata attccacttt cttccgctaa tttgattgcc
    15001 tcactatgga tatcagcatc cttgactaat tttgctgatt cttccttgat caataactct
    15061 aaagcttctt tgactgttac cgtccgttcc acttttttct tcggtgtcaa agcacctaga
    15121 gtttcattta ggtcgatccc catttgctcc aatccattgt tcatcattgg tgcaggtttc
    15181 ttttggtctt caacttcaat ggttacttca cgactatcca gtaaaccctt ttgtagttgt
    15241 tccaagactg tacggcgatt gattcgaata tcttctgtca cttcttcttg cggttcagcg
    15301 gtattttgct gactagcatt aaaaagattc atcatttgtt caaattgatt agtggattgt
    15361 ttctgttctt ttttgatacc aggtaccaag atttttacca aacgtttttc tgcatttttt
    15421 ttagcacttg tgtatacacg gctgtactgc tctttttcta cgatttggat cgcattttct
    15481 actaaatccc gaaccattga ttctacatct cttccgacat aaccgacttc agtaaatttt
    15541 gttgcttcga ctttgacaaa tggtgcattg acgatttttg ccaaacgtcg tgcgatttct
    15601 gttttaccga cccctgtagg tccgatcatc agcatatttt ttggtgtaat atcttgttgc
    15661 attttttcat ctaactgcaa acggcgatag cggttacgca aagcaacggc aacagatttt
    15721 tttgctgcgt gctgtcctac gatatattca tctaattcac ttacgatttg tcttggtgtt
    15781 tgttttaatt catacatatc tactcacctc ttacagttct tctacgataa tattatgatt
    15841 agtaaagata cagatatctg cggcaatatt taacgcattt tctgcaattt ctttcgcact
    15901 catgtcgttt tgagcatagt ttttcattgc tcgggctgca gccaacgcaa aattccctcc
    15961 tgatccgata gctaagatac catcatctgg tgtgataact tctcctgtac ctgaaacaag
    16021 aagcatttct ttatcattca ttacgatcag catcgcttca agtttttgca tagattgctg
    16081 tgtccgccat tcttgagcta attcgacagc tgcgcgttgt agattcccgt tatattcgtt
    16141 tagtttgcct tcaaattttt cttctaaagt aaacgcatct gcaacacttc cggcgaaacc
    16201 tacaacaact tctccattgt agatacgtcg tactttcttt gcagtgcctt tcatgacaac
    16261 agattcaccc attgttactt ggccatctcc agccatagca aatttcccgt ctttttcgac
    16321 ggcacagata gtggttgaat gaaatggtga ttcgaccatg tttgtttcct cctaaatttg
    16381 ttcttttcct tatttttacg ctcttggatg gaaagaacga tagttttttt gtaaactttc
    16441 ttttgttaca tgggcataga tctgggtagt cgataaattg gcatgtccta gcaattcttg
    16501 gactgtccgc aaatctgccc cattatttaa taaatgagta gcaaatgtat ggcgcagcat
    16561 gtgaggatgg atatcactat ccaaactgct ttttttgatc atttgattca agacatattc
    16621 aattccagta ggcgtgattg gttttccatg atgattcaca aaaacgatat catgctcttc
    16681 gtgatatttt gtcatcaata cctggcggcc ttccctgaaa tattcttgca gagcatcttg
    16741 agcaaaagaa cctagaggag catatctctc tttgttccct ttaccatgga tcaacatgac
    16801 ctctgaatca aaatcaatgt ctgataccga caaactgcta cactcactta aacggatgcc
    16861 tgacccgtaa aggacttcca gtaaagcgcg gttcctcaaa tccaaaggac tatctcctgc
    16921 tacgctgtca aaaagtgctt gcatttcatt ttcataaaaa aaacgtggca gtctcaaatt
    16981 tttctttttg agatgaacgt aagaaaatgg attttctttt atcacttctt ctttgagaag
    17041 atactgataa aacgaacgta ggctggctat cttgcgactg atagaatttc tgctatagct
    17101 aggacgatga tcagctaatg cactcaaata gacacgaacc gtaaaatatt ccgccttcag
    17161 cagatcagcg tctccagatt cctccaaaaa atgtttgaaa tggagaatgt cttcttcata
    17221 tgcttctttc gttttttctg aatatcctct ttcgacaatc aaataacgca aaaatttttg
    17281 ttcccacgtg gcttctgtca ttttctcacc ttccttttgt cccagagaat aatttagcac
    17341 agcgtttata aaaagacaaa taaattgtca gaatttaacc aatatttcta agtttgttca
    17401 caattggcgc aattcctcac attttgtttg agaaattctt aagaaaaacg caagaaaaag
    17461 agcgagacta tccgtctcac cctcttcatg gagtttcctt actcatttgg gattgcagtt
    17521 ttttctaatt ctgtttttat tgcctcatta gcttttaaag ctcgttgtgc aattgcttca
    17581 tatcttgctt ttttgtctcg aatgcgctct ggcagttcag gaaacagacc atagttggca
    17641 ttcatcggtt ggaaatgttt tccttccgca tgagtaatgt agtaagccat acttccaata
    17701 gctgtttctt gtggaaactc gactggttcc atttcttttg ctaagcgtgc ggcgttgatt
    17761 ccagcaagta gtccgctcgc tgcactttca acatagcctt ctacaccagt catctgacct
    17821 gcaaagaaca gatcttcgcg tttcgttgat tggtaagtcg gtttcagcaa ttctggcgaa
    17881 ttcataaagc tgtttcggtg catcacacca tagcgaacga attcagcatt ttctaaccca
    17941 ggaatcatcc ggaaaacacg tttttgctcg ccccatttca agtgagtctg aaagcccacg
    18001 atgttgtata aagaagctgc agcattgtct tgacgcagct ggataacagc ataaggacgt
    18061 ttgccagttt taggatcttc caatccaact ggtttcatag gtccgaaaag catcgttttg
    18121 aatcctcgct gtgccattac ttcaattggc atacatcctt caaaatattt ttctttctca
    18181 aaagatttaa gttctgccac ttcggcattg attagtgcct catggaaagc tttgaattcc
    18241 tcttctgtca tcggacagtt caagtaagct gcttctcctt tgtcatagcg ggacttcaaa
    18301 tatactttat ccatatcgat cgtagctttg tctacgattg gtgcagctgc atcatagaaa
    18361 taaaaaccat ctgaaccatt gaatgctttg atgctttctg ctaatggatg agaagtcaaa
    18421 ggtcctgttg ctactacggt gattccttcc ggtatttcag taatttcttc gtttatcact
    18481 gttactaatg gatgctcttt gacttttcgg gtaattgttt cagaaaaaga atcgcggtca
    18541 acagctaagg caccaccagc tggcactgct gtttcatctg ccgaagtgat tactaccgaa
    18601 ttcaatcggc gcatttcttc tttcaataac cctactgcat tagccaaact gtttcctctt
    18661 aatgaattgg aacatactag ttcagcaaaa ttacttgtgt gatgcgcctc agtcgatttg
    18721 actggacgca tttcatataa acggacaggg actcctgctt gtgcgatctg ccaagcagct
    18781 tcacttccgg caagccctgc tccaacaaca tttaccattt tcaatgaaag aacctctttt
    18841 ctattatttt tggatgtctt cttcgtaatc tccatttaca cagactactt gtttaccgcc
    18901 tttgactttc ttctcgacca gatatccgtc acattttgga caatttctgc ccactggttt
    18961 atcccaagaa gtgaattcac attctgggaa acgggaacaa ccatagaata gtctgttttt
    19021 cttagatttt cgttcaatga cttgtccttc atgacatact gggcaagtca caccgatttc
    19081 ttttacgatt ggtttcgtat tccgacaatc aggaaaatta ctgcaagcat aaaacttccc
    19141 atatttccct aattttatca ccatcggatg tccacagaca tcacaatcga atcctgctgg
    19201 ttcatctttg atttgaattt tttcgatttt ttcttccgca ttcgtcagtt ctttttcaaa
    19261 cggtttatag aaacgatcaa ccactttgac ccacgcttcc gtgccttctt caattttatc
    19321 caaatctcct tccatttctg ctgtgaaatg gatatcgacg atttgaggga agaattcaca
    19381 gattaatgaa ttgacaattt ctcctagttc agtcggctca aatctttttt gtgtcaattt
    19441 tacatagtag cgtctttgga tcgtatcaag tgttggtgca taagtggaag gtcgccctac
    19501 cccgttttct tctagtgcac gaatcaacgt agcctcacta aaacgtgctg gtggttgagt
    19561 aaaatgttgt tttggttcaa tatcaacaga ttggactaca tcgccttctt caagttctgg
    19621 aagaatattt tctttctctt ctttgccgtc atctctacct tccacataca cttgcatgaa
    19681 tcctttgaat ttgatttttg aaccgttcgc aatgaagatg acaccatttt gttctaatgt
    19741 cactttcatt gtatctaaaa tagctggtgt catctgacta gccacaaaac gtgaccagat
    19801 caatgtataa agtttcaatt gatctttatc taaatattgt ttgagttcat ctggtgtacg
    19861 aagcacactc gttggacgca cagcttcatg ggcatcttgt gcgccttgag catttttgat
    19921 ctttcggtga tcatgtctag aaaattcttt tccatacgta tcttcaataa atgctgcggc
    19981 ctctgctttt gctgtatcgg caatccgggt agaatcagta cgcatgtatg taatcaagcc
    20041 gactgttcct tgtttaccca aagtgattcc ttcatataat tgctgtgcta ccatcatggt
    20101 ttttcttgtt cgaaagttta acttacgggc agcttcttgt tgcaggctac ttgtagtgaa
    20161 aggattcgca gggtttcttt tgcgctcttt cttttctact tttttaactt cgaagtctgt
    20221 tccatcaatt ctagcagtga cttcttttac actttgtgca tctggtaatt tctttttctt
    20281 accgtcgatt ccccagaaat ttgctttgaa tttcttgcga gcttttttaa aattaccgtc
    20341 gatgctccaa tattcttctg gaacaaattc tcggatctct ttttcacggt caataataat
    20401 ttttaaagcg acagattgga cacgtcctgc acttaaccct ttcttgacct tacgccagag
    20461 aataggactg atcgaataac caacgattct gtctaaggta cgacgagctt gctgtgcatc
    20521 tactaaatct acatcgatcg ttcttggttc cttaaaagct gctttgactg cgtcttttgt
    20581 gatttcattg aagaccaccc gatttttttc tttcagatcc aacccaagta ggtacgctaa
    20641 atgccaagca atcgcttctc cttctctatc cgggtcactt gccaagtaaa ctttttgagc
    20701 tttttttgct gcggctttca ggcttttgat gacatcgcct tttccgcgta tagaaatata
    20761 atggggttca taattgtttt caaaatcgat acccatttta cttttcggta aatctcttat
    20821 atggccaaca ctggccatta ctttatagtt tcgtccaaga tatttctcaa ttgtcttggc
    20881 ttttgcaggt gattctacaa tcactaaata tttatacgcc attactgaat ggactccttt
    20941 ccattgtcgt gttcctacta ttaaaagagg attcatacaa atcgtagcta atcatatcca
    21001 ttcagaaaac gtttgtcaag aaaagcaaat gaaaagtatg gtgttattct ttaaattctg
    21061 ccaagatatc ttcacccgaa accgtgcaaa ctgcaccgtc ttggatcaaa tgatgacatc
    21121 cggtagaatg ttctgttaaa atatttcctg gaacagaaaa aactgttctt ccaagatcca
    21181 atgcttgctg tgctgttatc agagagccgc tttttttctt ggcttcaaag acacatgttc
    21241 ctttagacaa agctgcgata atccgatttc tcattggaaa acaatgcttt ttcgggccgg
    21301 attgaggagg atattcactc aaaagaagat gatcatttgc aatcacctct tgcaagtcac
    21361 gattttcagc aggatagact tgctctatcc ctgttccaat gactgcgatt gtccgtccac
    21421 catgagagat tgctgcttgg tgaccgtatg tatctatccc tttcgcgcag ccactgacga
    21481 tcacatatcc agcttctatc actggtttta gtaaataata aactgttttt ttggctaaag
    21541 cagaagtgtg tcttccacca acaacgctta acatgttaca agaaagtaaa gatagatcac
    21601 cattataaaa cagtacaatt ggcggatagg tcaagtgaag cagctgaggg ggatatttgg
    21661 gatcaaagca agtgataaac gactgtttcg attggatttt gccccagtta gcgtgaattt
    21721 cctgccaact tttgcgaaac agctcccggt gtttggttat tttacctatc ttaatgatgt
    21781 cttcaaatcc gatttcttta taaggatact gcatcatcca gttagcaagt gcccattttc
    21841 cttgactact gattcctttt gtataagcaa gtttcaataa attttcttct atttgataca
    21901 tcataaaacc gcctttcgtc tctctttcta ataagatacg taaaagcggc tgttttttat
    21961 tgtaaatatt tttttacagg tgcaaaactt ttacgatgga ttggcgtaat tccttgtcgt
    22021 tcaagtcctt ctaagtgttc ctttgttcca taacctgcat tatgcgagaa accatatcca
    22081 ggataaagca cgtcatattc tttcatcaat tggtctcgat atactttagc aatgatactt
    22141 gcagcagcaa tcgacacaga tcgtgcatcc cctttgatta ttttttcctg ctgaataggc
    22201 aaatccaagg tcatcgcatc gatcaacaaa tagcttggta caatggacaa tttttctact
    22261 gcttggatca tcgctaattt acttgcttca taaatattca catcatcgat tgtctgctga
    22321 tcaacgatcc cgatcccgat tgccagggct ttttcttgaa cttgttgata aagaagttct
    22381 cgctttttct ctgataattg tttggagtca ttcagcccta atatgatctc atttttcggc
    22441 aagataactg cagctgccac gacaggtcca gctaatggtc ctcttcctac ttcatcaatg
    22501 ccggcaatcc attggtgacc ttgtgcgtac ccttgctttt caaagcaaaa catttcttca
    22561 tatttttcct taattaatgc ttcctttttc aatcgtcgtt caaaagaagc cagtgcttgc
    22621 tgcacgcctt tgcgtggatc atttcttaat gattctagtg taggatcatc tattctctgt
    22681 atgcttttca atcgttcttt gatactttgg atggattcag tcatcacttt tttgctccat
    22741 ttcttcgcaa cgatccagtg tgtatcgtcc aagttttcct tgacgaatat catgcacgac
    22801 taattcgctg ccacgatcat agtcttcttt atacccacgt tttgctgtaa tatccatcag
    22861 taattccggt gccaattttt cttcttgctc gattgataaa ccataacgat cgctgattct
    22921 tcctgaataa tatcgggcaa aaaagtctaa tccgtaaatc gctaaatcat ctaaatggac
    22981 aagctggtct ttgatcgcgc ctgtcagcgc tagttttttg ccgatttctt catcttcaaa
    23041 tttgggccat aaaatccctg gtgtgtctaa caattccaac tcattaccta aacgaagcca
    23101 ctgctgacct tttgttacgc caggcttatt tcctgtctgt gcaattttct tgcctactag
    23161 tcgattcatc aacgtagatt tcccaacatt tggaatacct aagaccattg cccgaatagc
    23221 gcgaggcttg atcccccgag cagcatctct tgcccgtttt tctttcaatg cttctttagc
    23281 cgcaggtact agacctttaa ttcctttact ttcttgtgcc gtgatagcta atgcaagatg
    23341 gccttgttta cgaaaatatt cttgccacag ctttgtctgt tgtggatctg ccagatccgc
    23401 tttattcaat aaaacaaggc gaggtttttg ctgaatgatc tgatctagca ttggattacg
    23461 agaagataaa ggtaatcgtg cgtctaccaa ttcaaatacg atgtccacat atttcatttt
    23521 ttcagaaact tctcgtcggg cttttgccat atgtcccgga aaccattgga tggtcattgt
    23581 tctctccatt cctttcatac cggaatattc cccggcttta cttgtttttt tctttttcaa
    23641 tataaattca cttttttatt ataaagaaat tccatctatt agttaagtga aagtgaacca
    23701 gtcctcattc tatcacatct tcactttttt gatgatgcaa atttttattt tggtttctat
    23761 atatgaatct aaatttttga cagcgtttcc taaataagag atgatagacg aacaaaacag
    23821 aaaggagacg aaacaaatga atagtatggt ttttgttaat tttcctgttg ctgatgtcaa
    23881 tcgttccgtt gctttttatg aaaagctggg ttttaaaaaa aatgaagaat tttctactga
    23941 agaagctggt tcaatggtat gggatgacac tttttggata atgcttctct ctcatgattt
    24001 ttataaaaaa tttataaaag ataaagaaat agctgataat aaaaaaacaa gtggtgcttt
    24061 gatttcgttt agtgttgaaa atgcggatga agtaaaaaaa tttgctcaaa ttgcaaaaga
    24121 aaatggtggt actttccacg aagtagatat gggaatgcct gaagaagaaa tgttttcatt
    24181 agaagtccaa gatctagacg gaaatacatt agaacccgta tggatgaaga tgtaagcaga
    24241 taactaatct gacgtttttt ataacaaaaa aacaaaggcg gatgatacga taagtattct
    24301 ccgcctctat tttttttatt cgtacaatct cttttttaca gtttataaaa gcagtcttct
    24361 cacccttttt ttaattgtta gatgccagtg tatgtaaatc accaatgtgt catgacacat
    24421 tggtgatttt atggtatcct tttattatta actgacaaag gagagaatat tttgaacact
    24481 ttgattgcaa aagacgtttt tagattgccc gaaatgaatg caagattgtt gtcgacctct
    24541 gctcttttat tagcccttga tgtcatctta tacaagatag cgattggtcc tgcttatttt
    24601 caaatcacat tcggattttt aagtatggcg cttatgggtt atctatatgg accgatctgg
    24661 gctggtttgc ttgaaatgtt gtcgaacact ttaaatttca cgatatttgg ttcaggaacg
    24721 tttcaagctg cttttcttat cacggctttc cttggtggat ttattaatgg cttattttta
    24781 tatcataaaa agataaacct gccttccgtc atcgctgccc aattattgat tatgatccca
    24841 gtcagccttt ttatcaattc ttggctgatg tctgttttgt ttggtgcaga tttcaaagta
    24901 atttttctag gtagattcat caggaatatg atccaaatcc ctattcaaat aatggttgtt
    24961 tattcattta tcaaaacttt agaggctaga aaagtgttta aacgagctgt ttgattttac
    25021 gtactaaggt agtaaactga ttttggatta aacaataaag cgtgcagtct caacagactg
    25081 cacgctttat tgtttctcac tggatccatt ttatttcatc aagtggataa tagacaaatc
    25141 ttgctttacc tagaatatca gttttattga tcgtgccaaa cgaacgacta tcttttgaaa
    25201 ctcttcgatt atctccaagt acaaaataat tatcttccgg aagcttggat tgtcccgtta
    25261 gctcttgtag tgtaaagtcc gtcgtatagg atgcatttgc atgatctgat ttcaggtttt
    25321 tagttagata aggttcctta actactttcc cattgatctt caattgatca ttttggtagc
    25381 tgacattttc tcctggaaga ccgattactc gtttgatata aatcgtgcca tctgctaatt
    25441 gaaaaacaac aatatcaaat cgtctgatct ccgaaaattt ttccatcaca accatatcac
    25501 cttgcttcaa cacgttttcc attgaattgc cttccactgg taccgggatg aataaaaatc
    25561 cccgcaacat gaacgcaagg aatacagaaa caagtaagta tttgaataca agccagaagc
    25621 gattaatata gcgttgtttt tttgtcaatc tctatcaatc catccctctt tttttcattc
    25681 tttctttatt ataagaaaaa tatcaaaaaa atgcgactag atagctgaaa taaaaataat
    25741 tctttcaatt aggctattgg acaaaaaact tttttgatca taaagtacaa aactatctct
    25801 tatatgtcat gattattttc taaatatttt tcccatgctt cgtcaaagat atccatggac
    25861 ggcaaatagt cgacattcag ttccagataa ttagaaattt cttcgtagct agagctgtgc
    25921 ttaggaaatt ggatgtcttt accggcttct gtagcgaaaa cagataaagc atcatgtgtg
    25981 ccaccgcctc gcagtgtcat taaataatga aaaaagcttc gattcaccct tcgttcctcc
    26041 aattttcttc gataaaggca gcccggcgtg catggtttct tttaaaattt tcaggatgct
    26101 ttttataata ctcttgatgg tcttcttctg ctagataaaa tggttgggct ggttcgattt
    26161 ttgtaacgat tggctgttcg aaacgtccac tttcagctaa ctgtttctta cttttttcag
    26221 caatgatccg ctgttcttct gtcaaataaa agatcaccgg gcgataattg tcaccgcgat
    26281 cctcgaattg tccgaaagcg tcagttggat cagtttgctg ccagtaaagt tctactaatt
    26341 gttcatatga aatcaatgtt ggatcataga taatctccac tgcttctgta tgtccagtcg
    26401 tttggctttt tacttgttca taagtcggat ctggtacatg ccctccagta tatcctgaca
    26461 tcactgtatg gataccagga agcgtgtcga aaggctggat catacaccag aagcagcctc
    26521 ctgcaaatat tgctcgttcc attatgattc cccctctaat actactcatc cgatgatttt
    26581 ttattttctt ttgtcttgct ttcagtactg ctggtgctag ttttgttggt atttgagttg
    26641 tttgaagcat cagaggtatc ttttggcaaa tatagactaa accgaatgtc atcatttact
    26701 aagtcgattt tatctgcttt aatatacatc ccgttttgca ttcggaattg atccaaccta
    26761 agcatgatag tcagatcatc tgtattgact tcgatccaat ctggtaattt atagtttctc
    26821 ttgatcatat tcatcacatc tttgattggc aaactcaatg ttccgataga aagacttcta
    26881 gcttttagtt ggacatttcc gttttccatc acatacggat caaaatataa atagaaatcg
    26941 ataggaaaac ctagtacttc gaaagtacca ttgagtagtg cttcattttc caaataaaat
    27001 ttatattcaa tatcactatc tttttgatac tcactaagga aaaagctgat gatttgattg
    27061 actttttctt tatttgaatt gatcgttaag acaggatctc cttgtctttc cgtaagagct
    27121 ggtatttctg ggagttctgg ttcccgatta gcaaaaatac ggctgcctaa aaatacggtc
    27181 gaaccaagta ctaccccaag aaggataagg aaggcgaatt tccagtaatt tgttttcttt
    27241 gttttgtttg aagatacgtc agatcttgtt ttcatgttta ttgatcatcc tccttctcta
    27301 accattggtt ttgtgtctcg atcagctgat ctctcactgc atttgccatc aattgataac
    27361 ctaaattatt tggatggaaa tgatcttctt catagagcac attatttttg atgccattta
    27421 gattttctgt cgtgctagtt gtttgatctg ttccttcttc tgtgacacct acttcctgat
    27481 tcaatccttg ataaagcaaa tcattgattg ggataaagta gcagttcggc attttttttg
    27541 tcaccgtttc tgttcctttg ttccaattgt taacaattgt ctgcatgtca gtgatctcag
    27601 gaaagttcaa ataaaatgga ttgtagatgc ctaaaatgta aataggtgcg gttttattga
    27661 gggatcgaaa ttcttccaat agttctgtga cattttcctg atagtttttc agtggctttt
    27721 taaaggaatt gaccgttaac ccgaataaat tattttggat aactttcatc aaatcattac
    27781 cgccaacagt cagtgtgata aaatcagctg ttttgatatt gtttcgaatt tcttcttttg
    27841 tttttactcg ctttaagatc tggtcacttc gttcaccatt tacaccatag ttttctgttt
    27901 caaccgcggt caaattataa cggtctttta gatcatttgc gacaagggga acgaatcctc
    27961 cttgcttggt ttgatctcct actccttcag tcagcgaatc tccgatagct atgtagtgaa
    28021 gtttttcttt tttatttgga tcttctctca cagtagttac tggtttcatt agtgggccag
    28081 ctttcggtat agaagcagct aaacctgcat agatgatgat cgcaattaat aatgggaaca
    28141 aaacgaggat caaatgtcgt ttcgttaatt tcattttttc acctcttgta ttcgattggt
    28201 tctaatgtta tcaaaaattt tatcaaaaag gtagtcttta tgaaaaaaga aaacaattat
    28261 tttcctaatt ttataaagaa aaagagaata agctgcaagc ctttcaggct gagggcttat
    28321 cctcttttcg actaatctac ataatacatg attgcaaacg cgtctttacc agtatgcgta
    28381 gcgataaccg gattcgtatg cagtacaggg atatgcatgt ccgggaataa ttcttgcaaa
    28441 ccctctttga attcttgcgc caattctttt gcatccgcat gtgaaatacc gatttgtcgc
    28501 acatttggca attctttcag ttcatttttc aaattattaa accatttatc gaacgttttc
    28561 tttcctcgcc ccttagttac agggattaat tcagaatggg cgaaattcat caccacgcgt
    28621 atgttcaaaa cactagacag caaaccagtt gttcggctga ttcttccacc ttttaccaag
    28681 ttatctagtg tggatattcc gatgtataat tttgtattgt cttgtacttt tttgacttct
    28741 tcaaggattt tctctactgt tttaccttct tttgctaatt cagctgctcg aattacctga
    28801 aaagataatc cttggtcagt ggtatcactg tcgataaccg ttactttcgt ttttgttaga
    28861 ttactggctt gtcttgctgc ttctactgtt ccactcaagc cttttgtcat atggatcgaa
    28921 atcacttcac tgccgtcttc tcctagctca tcatacatcg agatgaattc tccaattggt
    28981 ggttggcttg ttttgggcag attttttgct ttcgccatca tttccataaa tctttcgccg
    29041 tctagttggt catcatcggg atatactgtt ccgtcgatca tgatggatag cggcatgaca
    29101 cgaatgccta aagcatcgcg tacatttttt tccatcgtac atgaagaatc tgttacgatt
    29161 tttacatttg tcattatttt ctctctctct ttctttaaca agatgcctta ttcatgttaa
    29221 actacatgta agacaatttc tatttttagt ataacagaca aataatgatt tttcattaat
    29281 tacacctagt aaaggagccg ttttcatgga aaaaacgaaa tttaatcgaa cgtatgaaat
    29341 attaaatgaa gtgtttaatg cagtcaccca tggaattggg actggattga gtatcgcggg
    29401 actagtggtt cttttagtaa aaggtgccca cttgcattcc tccctccata ttgtctctta
    29461 tgctatctat ggatcgatga tgattctttt atttttaacg tctactttgt ttcattcatt
    29521 gatttttaca aaagcaaaaa aggtgttcca agtattcgat catagttcga tttttctttt
    29581 gattgcagga agttatactc ctttttgttt gctgagtatc cgaggatggc taggttggac
    29641 tttatttgta ttaatctggt tgtttgcgat tcttggcatt atatataaag cgttgacgtt
    29701 gcataaacgg gagagagtat caaaagtatc tacgatcatc tatattttta tggggtggct
    29761 ctgtgtcgtg gctgctgttc ctctttatca ctctcttgga atgactggtg ttattttgat
    29821 ggtagcaggc gggctttctt acacgatagg cgcattcttt tatagcttga attctatccg
    29881 ctatatgcat gttgtttggc atttgtttgt tatgttaggt gcaggtttta tgtacttctc
    29941 tgttttattc tttacttaat aaaaaaaata aagctatgga tgggacagct ctaaataaca
    30001 gctagtctat ccatagcttt tttatatcgt caattcgcct gtcccgcttt ttacaggatt
    30061 ctcattacgc tgatacgttt caaattgatg cggataacga tttttttcat ccatttctcc
    30121 ttgactagta ttgatcagtg accaatcaga ccagtcaaca ggtggaaaat aagcatctcc
    30181 atcgaataca tgatggatga ctgtacggta taatatcgta caaaatggca gaaattcttg
    30241 ataaacagct gaccctccag cgatgaaaaa gataccgtct gtttcttttt ccagagcaag
    30301 cacgtcttcc acactatgca tgacatgcac gccttctgct ttataggaag aatctctcgt
    30361 caaaacaatc gtctgacgat tcggcaacgg acggctaccc atcccttcga atgtttttcg
    30421 tcccatcact agtgtatttg cttctgtcat ttgtttgaag aatttcagat catttggcaa
    30481 acgccaagga agacggtctt cttttccaat caatccgttt tcatcctgtg cccaaattgc
    30541 agctaacatt ttctcatcct ttctgattct aaacagcgat aggagctttg atcgccggat
    30601 gtgggtcata tccttctaat tggatatctt ccatctcaaa atcaaatact gactgctttt
    30661 cctgattcaa aactaaagtc ggaaatgcgc gtatttctcg gctcaattgt tctttcattt
    30721 gttcgatatg attgatataa agatgagcat ctcctaatgt gtgaacaaat tcgccaaccg
    30781 ctaaacctgt ttcatgtgca attaggtgag tcagcaacgc ataacttgca atgttgaacg
    30841 gaacgcctag aaacacatcc ccactgcgtt gatacaattg gcagcttagc cgtccctctt
    30901 gaacataaaa ttgaaacatc gtatgacaag gaggcaaagc catgctagga acatcttccg
    30961 gattccatgc agaaacgatc aatcttctag aatcaggagt ctttttgatt gcttcgatca
    31021 cttgtttgat ttgatcgata aagccaccgt cttttgtttc ccagtgacgc cattgatagc
    31081 cgtagatgtg tccaagatcg ccgtatgttt cagcaaagga atcatcggtt aggatcttct
    31141 cgcaaaaaag cttgctctgc tttttatatt cttcattgaa ttcaggatct gtcaaacatc
    31201 tatggccaaa atcagtcata tctggtccat tatagtctgg acttttgatg tagcgttcaa
    31261 atgcccattc atcccaaata tggtttttat tttgtaatag atagcggata ttcgtatctc
    31321 cttttaaaaa ccaaagcaat tcgcttttga tcaatccaaa aggtactcgt ttcgttgtta
    31381 acaaaggaaa ccctttttgc agatcaaacc gcatttggta tccgaataag cttaatgtgc
    31441 ctgtgcctgt tctgtcttct ttacgatgcc cttcttccaa caattttctt cctaagtcaa
    31501 gatacgcttg ttccatcctt tttcctccta atcttctacc cattcactca agtattccca
    31561 ccgtgccatt ttttcttcaa gttgtgcttc tgtttcagat acgtcgtttt gcaattcttg
    31621 taatcgagtg aaattatctc cttgatgatt catctcttct tggagcaatg agattttttc
    31681 ttcaagttct gcaatctcat cttcaatcgt ttcccattct ttttgttcca tgtaagataa
    31741 tttcttcttt tcagcaggtt ccttgctctg tatttttggt ttcgcttttt cagcaggctg
    31801 ctgggtctct ttttgcttca gtaaataact ggacatgttt ccgtaataaa gttcttgttt
    31861 tccgtttcct tggaatacta ataacttatc tgctaccttg tccaagaaat aacggtcatg
    31921 ggaaacagtg atcacagcac ctggaaaact tctaaaataa tcttccaaaa tcgtcaatgt
    31981 tgctatgtcc aaatcattgg tcggttcatc caataataaa acattaggtt gctgaatcag
    32041 cagtttcaac aaaaataacc ggcgtttttc tccgcctgaa agtttgccta tcagtgtccc
    32101 atgcataaat cttggaaata aaaatcgttc aagtaattct gctactccga tttgactgcc
    32161 atctgctgtt tttgcttctt cagcagcttc ttgtaagtaa gcaatcatcc gtttatctgg
    32221 tgccatttct tcattttctt gtgtataata cgcaagatga accgtttcac cgatagacat
    32281 tgtaccgcta tccaggggaa ttctgccagc tagaatatta agcaatgtcg attttcccgc
    32341 accattttta ccagtaatac ctagtctttc ccgagattga atcaataaat ctaaatgttc
    32401 tagcagtgtt tggtcattta tacggtaact gccatctttg atttccagta cttttttccc
    32461 taaacgttgt gtcgctaggt tcagctctaa atcatcttct tggtttactt ggtacaagtt
    32521 ctcctttaga tcttcgaaac ggttgatccg tgcttgctgc ttggtgccac gtgcctgtac
    32581 gccagccctc atccaagcta attcctgttt ataaagccgc ttgcgctttt cttcttgttc
    32641 tacttctaca cgatcacgtt ctgctttttc taagacgtaa gcttcataat ttcctttata
    32701 ctcgtataat tttccaaatg aaagttcgaa gattcggttt gctactctat ctagaaaata
    32761 acgatcgtgg gtaaccatca atagtgcgcc acggtattgt ttcaaatagt tttccaacca
    32821 ctcgatcgct tcataatcta aatggtttgt tggttcgtct agtaatagta aatcgggttc
    32881 atcaatcaac acttgcgcca gactaactcg ttttttctga ccacctgata aagtactgat
    32941 ttttttatct aattcactga ttcctaattt ttgcaaaata atctttgcgt tggtatctgt
    33001 agtccaagca tcttctttat tcatttgttc ttctgcttta gcataacgtt tttgcacatc
    33061 agggtcttct ccgttttccc ctagttcgat caatgcttcc tcatatgctc gaacagtttg
    33121 gatcaagggg ctgtttcctt gaaagactgc ttggagaaca gttaattcat cggaaaattc
    33181 agctgcttga gaaagataac caatacggta atcagttgga taaaacactg tttgccgatc
    33241 accgtcgctg ctatcgatac cggcaataat atttaagaga cttgttttac ccgtcccgtt
    33301 cgtcccaatc aacccgatac ggtctttttc gtggaccaaa aaagaaatct ggtcaaacaa
    33361 atctttttca ccataagttt ttttgaggtc agttactttt aattctttca acatgttccc
    33421 tccttatctt ggatattgta tcataaaaat agtgaacttc aggaaaattt ttttggtatt
    33481 tgttcttttt gtgttctcaa aaaaagaaca ggaccgattc gatcctgttc tgatatgttt
    33541 tgttttttat aaaacttgaa aatttttacc tttttatttt attggactcg ttttttttga
    33601 ctttgctcga aacgcaaatc ggaaagaaaa gttatcagat ggtttgttca tcgtataaga
    33661 ttctaacaaa ttgatattcc tgcgatagct gttcgcagaa aacgtcgtga ttttttcagc
    33721 aacttcgtat tcatcaatga tttttctttc aagataataa cctcttagca tttttttcgt
    33781 aaacagacta tcttgctgga tcatccacga agaaatcaaa ttccccgagt tcatttttat
    33841 tccttcaagt ttgcgttgct ggataaaaaa ttgaaccatc gtttcatcat aaatatcagt
    33901 caagttatct aaactttcaa taatcaactg agtatttttt tgatatgtct ctctgatttc
    33961 ttggatatct tcatcactaa taaatgtctg acgatcagca tatcttcttt gccaaaacat
    34021 tttcggatgg agaacgattc gcattaacct acggacaaca atgaaccaaa agccgataaa
    34081 agacaaaatc tggcttgtga tagaatgttc gaatcgagaa agaaaacggg agtaaaaacg
    34141 ataaccatta gaagaaagct tgcccgaacg ataactttca tccaatccat cccgttcgat
    34201 cgacaaaatc agtgcttgta tttcttgtac ttcttgcttt tcatcatcgg ttaaatcttc
    34261 caagtacaga tcacgtatcc gttcttgata attttctatc actgcttcag tggctaaaaa
    34321 ttcttttgta ttacgatcaa tttcctttat ttcttttcgc aaaacttcaa cgacctcttc
    34381 taaaatcatc aaagcattca aatccgtgct tttcgcttcc tctgtttccg tcagcatagg
    34441 caagacaatg atccctatga ccaaagtcac taagatgaca cccgccgtca gaaacaaaag
    34501 caaagaccgc tggtaaaaca tcatattatt gattgaaaat ggcaaaataa aaatcgtagc
    34561 caaactgact gttcccttta caccgccaaa tgttaagatc aagatctcgt tcaactgttt
    34621 tttgaacttt ttactgccat ctttgaatac ataaaaaaga ctgatagaaa tgaatctgat
    34681 cacaaaaagc atgacagaga ttaatacgat aatcgctaat aaaagaccgt ttggatataa
    34741 accgtctccc catactggcg agaacacttg cgtcaattcg attcctaaaa atataaatac
    34801 taatgcattc agtgtaaaag caatagttgt ccatgtactg tgggacaagc tcgacaattc
    34861 tgcatcaaac acagagattt tcctaaaacc agaagcttgg agaatacctg ctgccactgc
    34921 agcaatgatt ccagatacat caaatgcctc tgccaataca taggctagaa aaggaagcaa
    34981 aagttcgatc agcagatacc ctgtgacatc ttgtgctgat gctttttcga tcaaatgaat
    35041 tattttttgt ttgaaccaga caagcaaaaa gccgatcaaa gctcccccaa tactagaaac
    35101 tactagactc atccccgcgt ttactgctga aaaagatccg gtgatcagcg caccaagagc
    35161 aaactggaag gcagtaacac cagatgcatc attcaaaaga ccttctcctt ccaaaatatg
    35221 catcgccttt ttagggatat tcactcgtcc agaaagagaa ctgactgcta ccgcatctgt
    35281 cggacctaac gctgctccaa aagcaaagca ggcagcaagt gggacacttg gcaacaggaa
    35341 tgataacgtc gcacctaccc cgacaagggt caaaatgact cctccaaatg ccaagaacaa
    35401 aattgtcccg aaatttttga gaatcgaaga aatatctgct ttttctcctt ctctaaataa
    35461 caatggtgca ataatcataa ctaagaacaa ttcaggttca aaatcgattg attgtcccca
    35521 ctctgtcaga cctaatacca ctccaaggaa aatttggatc agtggagctg gaatcattgg
    35581 caaaatgcgg ctagctatgt tcgataaggt gatcgttact gcaaatacaa taactaactc
    35641 agcaaattcc atcgtccatc ccctttattt tttattttgt gtaagcattt ttaaaaattg
    35701 ttcttttttc tttaaaattt tggtttcgac tacttcaacc tgtgtttggt tcccctctaa
    35761 ttcacactgt acacgttttg actccaaacg aagcagatca tgtttcagac gttccaactg
    35821 acgatacatc aatatatttc ttttacgcaa cttacaaagt atttgacggc ggtcatccat
    35881 cgaaagggcg ccatagcgat catgcaacag ttttttttgt tgttcaaaac tctctctcat
    35941 tttccagatc tcctatacac aaacttccat cattatagca aagttctttt tggatgaaaa
    36001 atatttcatt gtttcatcgt ttattgctag ttagaaattt ccataaattt ttcaacgcct
    36061 cattctattc caaatagtat cagatatcaa gtaaaactta agaaagataa aagtttctga
    36121 caaaaggatg tttactttcc cattcctgcc tttcttgttc ttttatcaca acaaaaaaat
    36181 ggggctgcga caaaaatctt gtcacagtcc cttattctga ataaacggtg tggaagagac
    36241 agcctatcag tagtaataag acacatctct ttcacagcct catttttgcg taaaaatcga
    36301 tttattcgaa aaattccccg tatacatctt ctacacgttg atctggattg atcacgatat
    36361 acaagttacc tgttttttcg ctgatttttg ttgattgata ttgattgtct aatccttctg
    36421 ttcctttatc tagatatggg aagtacacaa gatcaggtgt gccatttcgg taaaggattt
    36481 ccattcgctc gatatcttcg tatttcaatg cgcgattaaa catgcccatt tccagtccgc
    36541 ctaggttgat atcatgtgtc gcaactgcgt ccccttcatg gaagatttcg attttaaatc
    36601 cagcacacgg atggatttca acaaattcac tgccattgat tcgtccaaaa cttgttgtga
    36661 tttgtttgat ccaaagatcg ccgatatgac ggcggttgat cgtccaggtt tctccatttc
    36721 taaaataaaa ttttagcgct gtcatttctt tactcattaa aaagactcct ttatgatgta
    36781 atttattctt gtactacctc agtttagcac aaatttatgt aagtggtcac atcttatgtt
    36841 ttttcttcta atgacgtatc tgcccacttt aagtagccaa caaagctgag cagatacagt
    36901 ctagtgtttt ttaaatcaat ttttctttcg ataaaaaatc caaaacctgt tttttatcat
    36961 tttttattct ttgttctacg acggcttttt cagctaattg caaagcttca ctaacccact
    37021 taccaggttt ttttccagtt tcttctagca agattttacc atctactgct aattcgctta
    37081 atgacttgat aggtaaacga tcataggatt ctcctacggc atccatctga ggtgtttcgt
    37141 caaaatagat catcaattct tctacttgca gtgcatgttt tttgcctaag aagtacaatt
    37201 cgaatggttc caaagctcca gtttctcgtt gtcgtagtcc ttggacaagt gcttgaacgt
    37261 ctttaatcat ttggttagaa cacttccacg atttcaaaaa ggaacgaatc tcattttctt
    37321 ttagatccag ttggctgatc aataatgccc atgcttggct ctcttctttc aatgggacgg
    37381 atggtaattc agcaaaacgt aaaagtgctt cgccttcttc tttcaaaccg ggacagcaaa
    37441 tgtagcactc tgtttcaata aatgcatgga ttcccgcggc ccgttcattt cccaagagca
    37501 tcttgatgaa ttccacattt atacgttcta cagatatttt acctaatagc tcgtggtttt
    37561 ctttgatggc gtagaaggtt tcttcctcta atttgaaccc tagttgactg acaaaacgca
    37621 acccgcgcat cattcgtaag gcatcttcat ggaaccgctc atgaggattg cctacagctc
    37681 gcaagatctg ttcttctaga tcttgcagac catcgaacaa atcgatgatt tctccatctt
    37741 ctttcaaagc aaacgcattg atcgtaaaat ctcttctttt caaatcttct gccaacgaac
    37801 gaacgaattc gacatgatct ggtctacggt agtcctgata agttgattcc gtccgaaatg
    37861 tggtgatctc atattgttca tcattttcta ataccaacac agttccatgt tcgatcccaa
    37921 tatctatcgt ttttggaaaa agctgtttga tttcttctgg aaatgcgctt gttgcgatat
    37981 ccacatcatg gattggctgg ttcagcaaga tatcacgaac acttccacct acaaaataag
    38041 cttcatatcc tgcattctct aattttttca gaactggaat tgccttttgg tattccagag
    38101 gtaattgttt taatttcatt tttccaacct ttcctgcgtg tttcttgtcc tgagataaag
    38161 actcagttgc atggtgactg tttgacaagt ccgtttcaga gtcttcaagg ccaggaccac
    38221 ttcatcttgt ttactcgtcc ttacgctcaa agcgattatg gtctctttga ttgaattttt
    38281 ccatgttttt ttcaaaaact tcagttaagt cgatattcaa tgaattagcc atgatcatcg
    38341 taacgaataa aacatcacca agttcttctg ctaccgagtt aactggttca gaagcctttt
    38401 tgcttttttc tccataagtg tggttgactt ctcttgccaa ttcacctact tcttcagtta
    38461 atcgagccat ctgagctaat ggagagaaat accccacctt gaattgctgg atatattcat
    38521 ccacttcctg ctgcatactg tataaagaac gctttttatc catctttttc cctccaaaac
    38581 tatcttacca aaactttaac gcaaatccta gataagagca attgagaaaa tcgaatttcc
    38641 atgctacatt taggaaggaa tttctaaatg gaaattctgt gaaaatacat aattgaaagc
    38701 tggaattcat atggtcaaac gaaaaacagt aaaagaagtt ctttttatca ttataggaac
    38761 aagtatttat gcatttggtc ttgtttactt gaacatcgct aatcatttag cagaaggggg
    38821 agtctcaggg atcaccttga tcttaagagc tttatttggt attgatccag cttatacgac
    38881 tttattaatc aacatcccat tgattttact aggaggaaag atcctcggta aacgttcctt
    38941 ggcctatact gttctaggta cactttcttt gtcattcttt ttatggatct ggcaaagggt
    39001 tccactagaa atcaatttac agcatgatct actgatcgtt tctctacttg cgggtttagt
    39061 tgcaggtgta ggaagcggga tcgtctaccg catgggtgga acaacaggcg gcagtgatat
    39121 tatcgctcgg attttagaac aaaattacgg catcactatg gggcgttccc tattggcatt
    39181 tgatatcatc gtcttacttg cttcattaac ctatatcgac ttgaaacgta tgatgtatac
    39241 gttgattgtt tcgtatgttt tcagtcgtgt gattgattcg atcttagatg gcggatattc
    39301 tgcaaaaggt ctacttgtcg tctccaacaa aagcgaagag attgcaccac tactgatgac
    39361 cggtctgcaa agaggtgtga ctttcctttc tggagaaggc gcattctctg gtaattcaaa
    39421 aaaaatcatc tatatggtcg ttagctctag tgaattaact gaagctaaga ggatcgtcca
    39481 cgagattgac gaaaaagctt tcctttcaat catcaatgtg catgaagtgg aagggcaagg
    39541 ctttacttat ttgaagccac agacaagatt actgaaaaaa ctataagaaa aaagacaaag
    39601 ataggctatg aggaaagatt ccacatccag ctgacctttg tcttttttag tactatacta
    39661 tttttaacta attgttatgt tcacttaccc ggttactttt cttttgtgtc tgcaaaagga
    39721 tctttcggtt aacttcatca gataatttca aaaaagcatc cctatctcca tcttctagtg
    39781 cttgatcgat ttgagcataa agcattttta gttttgcctc ttcttctttt tcttgaaaat
    39841 atcgttcaat taattcagta tcttcttcac tgactgaaga atcccaagaa gaaaatggat
    39901 tgtcttctaa gatagataga tattctcttg tctgccaagc attttcgaat atacattcca
    39961 catataaagg ctttttccaa ttcaaacgga tttcatgaaa aatctggtct gtgtccgtaa
    40021 attcttttcc ctgcaaaaac aatttcatgg gttccccttc aacagataga tcactgatct
    40081 gcagtcctcg atccgcaaaa ttcgcccctt caacaaagtg gacattgctt aaaatcgctt
    40141 catgattggc aagataattc aagatccaaa aaacttctct tcggctaaaa gatacatgat
    40201 tgaccatcca cgttaaaaaa ttcttctttt cccgtacatc aattagcatt tttctcacct
    40261 ctctgttagg tcatctaata atgacagtgc ctccatatct cctggttcaa gtgttaaata
    40321 ataagtcaat aattcttgcg ctcttttcag ctgcccttct tctcttaaga aaaaggcata
    40381 ctctttcaag aaatctggtt catgtgatag ttcatgataa gcctgttcat agtgtacagc
    40441 tgcaacggta aagtcttcta attcattata agcatgggct aggttccatt ctgcataagg
    40501 attagcagtt tcttccatct gattgatcac ttgtatcaca tcttcatatc tttcatcatc
    40561 caagtaaaga ttacttaaag taagtaatgt ttcatcttgt ttgtctccta cttctaaagc
    40621 tttcagtaaa agttcttctg ctcgctgtct atcatgcaga cggaaagcat tttccgaggc
    40681 caggtgataa agttcgactt ggtaaggatt ttctttgatt ccttcttcta acactgtttg
    40741 tgcttcttca atcatttctt cttcttgaag agcttgtcct agatatagat ataacgattg
    40801 ataatgcgga ttaacttctc gaagttcttc cagatagttg attgcctgct gattttcttt
    40861 caattgaaga ttcgtaaaag ctagctggaa cagtcgatca tctgtccttt cttctttcag
    40921 tgcttcttct aaaggtacga tagctgcttc gaattctcct tgcatactta aggattgtcc
    40981 taaacgttcc tgcatcgaga ttcctgaaat ttcatttatc ccgcttgcca acaatgtttc
    41041 ataaaccact gctgcctctt tgaaacgatc aactgaaaaa tacaattcag ctagcgcaaa
    41101 ctggatcaat ggttcatcag gaagcaaccc agctgcttct ttcaattttg cttcactcac
    41161 ttcagggatt ccgataactt ggtacaaatc agctatcgcc aataagcttt gcggatagta
    41221 atcactttgc ttatccactt tttccaggta ttcaaaagct aagtcgatct cattatcttc
    41281 gattgcaatt tctgctaacg ggatgttcag tccgtcattt tctgggtatt ggttgatcag
    41341 ttgttcaaaa atctgttttg cttcgatcaa aaagccaata gccagcaatt cttcgcctag
    41401 ttcagctaat atatctgctg ggtcttcttt taatgctttt tctaaaaaga tttgtgcttg
    41461 tgacaagtct tcatctgata aagcttgcaa cattttttcg ctatttgtat tcatttcaat
    41521 tccctcttat ctcactgacg atttgttgaa ataccgtcat tgttctcaat aattcttttt
    41581 cgtttggatg ttggtatccg tctattatac ctattttttc taaaacaagt atttttttgc
    41641 ttttttctgc caaaaaaaga actttttcca aatactccat ctttgaaatc tgttccagcc
    41701 agtggattgg atagccaagc aagtccagcc acacaaaaaa gttttttagt tggaaatcaa
    41761 aaggatatgc ttggagattc cacacgaaat gaaacatcat tcctaaaaat tttttcatac
    41821 tagcagaaag tagatggcca ttttcggtca gataaaaagc ttgttcaaac agtttaccat
    41881 aatttgacag gttttctgcc tctaattgat aaaaatagat catttcttcg atcatcccgg
    41941 caaacggccg agtcgttaat tgctttttat ttggatagtt tttatacaga ttttgcaaaa
    42001 aacgatgatc acaaatcatt ccgcaacaga taaaagtcaa aaagtcaatc tgctttccgt
    42061 ccacctgccg ctcgctgatt gtttgatcat aaacgatgcg ttctggcaga ttgatggttt
    42121 gcagcactac acgatttggt agctgcaaaa tcgtccgctg cggtgttaat gctcggctta
    42181 gagaacggat cgatacaggg atcatccata gtgatgaatc aagggttgta tgtttttgaa
    42241 aaaaacctgc cagatccatg atcccttcat ttccaaatcc gatgataaac atggattgat
    42301 tttcgggata gcgtttgacg aaatcaagca ggttctttaa ttctgttaaa tgattgcatt
    42361 gagtgttggc acaaatatac cagtcattgt ttgcttgatt tgcaaataac gggttgatct
    42421 tatctgcaaa caaatcataa tagcgctgat tcgttaaaaa taagattggc tgatcaatgg
    42481 aaagttctct tagctgactg actagcgttt ctccgtaaaa cactttcgtc aacgcatctt
    42541 ctacttgata atcaagttcc atacgcatcc ctcctcttct tatttttatt ttaccattag
    42601 taagcaaatt ctagaaaaga taagagcaaa gctcacctca taacttgcaa ataaataaaa
    42661 attaaataaa atacatgtgt tttctatgta tgacatacgg attcggaatc tctgtctctt
    42721 atttattcct tttctgctag gaaataaaaa gaaaagcact tgtagcccta gagccacaag
    42781 cgctttttca actgtcttat ttaacagcgt cttttaatgc tttacctggt ttgaatgcag
    42841 gtactttgct agctggaatt tcgatttctt caccagtttg tgggttacga cctttgcgag
    42901 ctgcgcgttc gcgaacttca aagttaccaa aaccgattaa ttgaactttt tcgccgttag
    42961 ctaaagcatc ttgaatagtt gaaaatacag catctactgc tgcagttgca tcttttttag
    43021 ttaaatcagt agctgaagca actttttcga ttaattctgc tttgtttgcc atggatagat
    43081 tcacctcctc cacaagagat atgatagaaa tatcatttag ttaaaacttt ctgagtggtc
    43141 cagaagttta aaaagaaata ttgattaact tcaatattct gttatcacga tatcatatga
    43201 accgcttaat agcaaggttt ttaccaattt tcacatgctt tttttagtca tagaaacgta
    43261 acaaacgaaa ctgtcttttt cttttgaaaa aaaaacgtcg atatggtaaa atgcctattt
    43321 tctcctacga gggataattc ggatggacgt cccttcaaaa gtaaatgctt ttctgatttg
    43381 attttctaag aaacgttcat aagaaaaatg catcaattcc tcttcattga caaaaacgac
    43441 aaatgtaggt ggtttgacag cgacttgtgt agcataaaaa atcttcaggc gttttccttt
    43501 atctgttggt gttggattga ttgctacagc atccataatg atatcattca acacagccga
    43561 agagatacga agattctggt tcatacttac ttgttcgatc atttcaggca gacgttccaa
    43621 ccgttgttta gtcactgcag aaacaaaaac gattggagcg taatcaagat accggaattc
    43681 atcacgaatc tcttgttcga aatcacgcat tgtattggtt tctttcttta caagatccca
    43741 tttattcacg acgataataa ttccacgacc agcttcatgg gcgtaacccg ctactcgctt
    43801 gtcttgttcc cgaatccctt cttcagcatt cagtaccatc aaaacgatat cagaacggtc
    43861 aatggcacgc attgcacgca tcacgctgta tttttcagta ttttcgtata cttttccgcg
    43921 tttacgcata ccagctgtat ctatcattaa gaatttctga ccactttcag aaacgaagtg
    43981 ggtgtcgatt gcgtctcggg tcgttccttc aatatccgaa acaattacac gttcttctcc
    44041 taaaatcgca ttgatcaaag aggatttacc aacatttggg cgaccaatca aactaaactt
    44101 aatcgtgtcg tcgtcctctt cttcagtctc ttcagagaaa tacttcactg cttcatctaa
    44161 aatatcacca atccctagtc catggcttcc tgaaacagga taaggatcgc ctagccctaa
    44221 ggaataaaat tcatagatat cgtttctcat ttctggatta tctactttat tgactgctaa
    44281 aatcactggt ttattacttc tgtataaaat tttcgcaacc atctcatctg cgtctgtcac
    44341 gccttcgcgt ccgctgacta cacaaatgat tacatctgct tcttcaatcg cgatttctgc
    44401 ttgatgcttg atctgatcca taaaaggttc atcgccaaga tcaattccgc ctgtgtcgat
    44461 cacgctgaat tctcgaccta accattcacc ttttgcataa atacgatcac gagttactcc
    44521 aggagtatct tcgacaatcg agatacgctc accagcgatg cggttaaaaa tcgtagactt
    44581 accgacgttc gggcggccaa cgattgcaat tgttggattt gccatattat ttcctcctca
    44641 caagtttcca atctcactta gtttaacgaa tgcattgcca actttcaagc atagattgtc
    44701 aattaaacga agtattttta ttccttattg aaatactaat cgcttttcaa aagaaaaact
    44761 gagacaaaag cagctgcttt catctcagtc acagatacaa aatcaattat ttttcagaat
    44821 catcggttgc tttttcttct acttcttctt tcaacgcatc acctaaaatg tctcccatag
    44881 taaatccagt attttcttct ggtaattcat aagactcaac attttttggt tcttcttctg
    44941 aagctggttt ttcttctaaa gctttgatac tcaatgcaat acgatgttct tctggatgga
    45001 tctctaatac tttcactttc acttcgtcac cttcatgaag cacttcatgt ggtgtcgcaa
    45061 tatgtttatg tgaaatttga gagatatgga ctagcccttc tactccaggg aacacttcaa
    45121 caaaggcacc gaagcttgtc aaacgtttaa ctgttccgtc taatactgtg ccaactggag
    45181 cttttgtatc gatttctgac caaggtccag gctgtgtgtc tttgattgac aaggaaacac
    45241 gttcttgttc aggattgata gacaacactt tgacatttac atcatcattg acagccaata
    45301 catcacttgg ttttgctaca tgactatgtg aaatctctga gacatggact agaccgtcaa
    45361 ttccgcctag gtcgacaaat gcgccaaaat cagtaagacg tgcaacacgt ccactgagaa
    45421 catctccctc atggatagtt gaaagaattt cttttttctt ttcttctctt tcgttttcca
    45481 ctaccgcttt atgcgataag atcaaacgat tttctgatgg ttcgatttcg atgattttaa
    45541 agctcaatgt tttgccttta tagtctgaaa agtctgcaac aaaatgatct tccaccattg
    45601 atgcagggac aaaaccacgt acaccgacgt caacgactaa accgcctttg acaacatttg
    45661 taacaggtgc ttcgatgacc ttgccctctt ggaaatctct ttcgatatct tcccaaactt
    45721 ttttagcatc taaacggcgt tttgaaagta gatagctgcc gttttcttta tctttgccga
    45781 tcgtactgat aacgactaaa tctaatacat caccgacttt tactacttcg ctgatatctt
    45841 ctaccggcaa agttgataat tcttttgccg gaacgactcc ttcaactcca gccccttcga
    45901 ttccgacaat cacttgtttg tcttcaatga ctaaaacttc gcccttaaca acatcgccaa
    45961 cttttacttc ttgtacgctt ttcattgcgt cttccattga ctggttgttc tcttgatttt
    46021 gatcaaattc tgtcataaca cttgtcctcc taaccaacat tctgccgtaa gtacggtact
    46081 gtttttagtt taattgatta ttcaaaaaaa ataaagcgat ttcgttcgtt ttccttgaaa
    46141 aaacaattgt ttttcatttt ttttcttaat ttaaaaaaga agataacctt ttttcaaaac
    46201 aacagctttt attgctgcaa cgacttctgg aatcgatttg ccagtcgtat cgatacgaac
    46261 agcatcctct gcttgcttca gcggagagac gtcccttgtt gaatcataat ggtctctttc
    46321 ctgtatctct ttcgtcagtg tttcaagatc agtcggaatt cctttttctt ggttctcttt
    46381 gaaacgtctt tctgcacgct cacttacgct tgctactaaa aagattttca cttctgcatt
    46441 cggtaataca gctgtcccga tgtctcttcc gtccatgaca acgccaccgc gttcccctat
    46501 ttttcgttgg acagcaacta attcttctct gacttttcca tgtgcagaga cttcagaaac
    46561 agcttttgtg acatctggtt gacggatttc taaagtcaca tcttttgatg ctatgaatac
    46621 atgctgtcca tcttcttgtt gcgaaaaaga gataggatac tttttgatca gcgaaactag
    46681 cccttcttca tctgaaaaag atacattgtt cgtaatagct aaataagtta ctgcgcgata
    46741 catggcaccg gtatctgtat aaatataatt caattctttt gctaatatct tggcaacggt
    46801 acttttccca gatgaagcag gcccgtcgat tgcaatactg atttgtgtca tgatataact
    46861 cctttttctc agaaaaaaag ccgcaaagcg cggctattat tatttaattc ttaattcttg
    46921 accagggtaa agcataaagt tgtttggatc taagccattc aattcaaaca attgatcagt
    46981 ggtgatccct gcacgctcag ccacttgttt tggaccttcg cctgcttgaa cagtcgttgt
    47041 tgaaccggct gcagctgatg aagaaggtgt tgtttctgct gcattgctgc ttgaatcccc
    47101 tgcttgataa gttgcctcac ttgaggaagt cggtgttgtc tctgctgcaa tactgctttc
    47161 tgacgttaca cctgtatctt gagacacagt agattcttcc gcgattgtac tagattctac
    47221 tgttgaagtc tcagttgact cctcttttgt tgtacttgta gaactttcta ctactgatgt
    47281 cgaagtttca gaagaagttg ctgctgttgc attgttgctt gatccatttc tgatccagaa
    47341 ataagcacct gctggaatag cgatacataa gaccagtaaa atcaaaaata gcgaaagaaa
    47401 taatgtattt cctttctttt gttgtctctg agaacttctt gatgaaactt catcatctgt
    47461 atcgtagatt ggttgttccc atggttcttg tgtttcgtta ttttctgtag attgatgtct
    47521 atcttttctg ctcactgttt tacctcctaa actctatcgt gtgtattata ccataggaaa
    47581 aagtgtcttg tcctaaaaaa tcaatgttcc atgttttttt taaataaatt tagtaaaatc
    47641 gttccccaat gaagttgttc agtcttagaa aaaagcttcc tctttggcag aagttctgct
    47701 tttgctccat caatatcaca acaattctgc ggcttttcgg agagtttttc gccaaaataa
    47761 gctaagatga attttctgcg gcagttttct tcattaatat agcgaagcat ctgctgcagg
    47821 cggaattctt tttctttttc ctgacgtttg agccgttcca cccaattttc tggtttttca
    47881 gactgctgga tcagctccat ccattttttt tgcagctcat caaagggagc ttgctctgtg
    47941 gcatattcca aataaagctc aaaactttgt cgctgttctc tagaaagttg attaaagaaa
    48001 taatggatac gttcatcccc actttgataa aaaagaattg ctgcactttc ttcctgatct
    48061 ctgcctgctc gaccgatttc ttgtacatag ttttctagac tgtctggcaa atcataatgg
    48121 accacataac ggatatcaga tttatcgatc cccataccaa atgcattcgt agcaatgaga
    48181 aactgcagct gattttttac aaactgctgt tgaagttgtc ttctttgagc tgcatcaaga
    48241 ccaccgtgat agtatccaac tgtaaaacgt tccctgaata aatgatacaa tcgttcgact
    48301 tctttttttg ttgcacaata aatgattgcc gctccttgtg ctctttccat gaagtgttca
    48361 agttcttgtt ctttttgctg cgttttccga acaaaaagcg agatattttg ccgattgact
    48421 gattgtctga tctcttttgg tggtcgatca aatagtacat gacgtatatc tcttgctact
    48481 aaatctgttg ctgtcgccgt tagtgccagt gtgaccgggt ttcctaaaag ctttcggatt
    48541 tttcctaact gttgatattc tggacggaaa tcaactcccc attgagacac gcaatgagcc
    48601 tcatctacta catataacgc aatattttgt tttttcagat gctctaaaag agaaggatta
    48661 gtcaacattt cgggactcaa aaacaaaaat ttatattgcg atagatgacg gaggacgtat
    48721 cttctttcag attgcgaaag taaactattg aaagcagcta ctcgtttctc ccctcgtttt
    48781 tgtaattgcg tcacttgatc ttccattaga gaaagcagcg gtgacacgat gatgaccaga
    48841 ccttccatta aataccccgt caattgatag caaaggcttt ttcccgttcc tgtaggcaaa
    48901 atagcgagag tatcttctcc atcaagcaac gcctgaataa tttcttcttg gccgggacga
    48961 aatgaatcaa atccaaaata tttttttaat tcatttatta acattctgta tcgcttcctt
    49021 tctcaataga tagatttgat acaaacgaaa ttctccataa tctaattgtc catctacatt
    49081 ccattcgcta aagcgccact ctcgcacgga atccttattt ggttccaaac gttcgattgt
    49141 ttctgcagag agaatccact cgtatggaaa atcatcaaaa aacaaggccc actcaataag
    49201 atgatctgtt actgttcctc tttttaaatg acggattgcc atgatctggt caattgtctg
    49261 accattcata aacatctgct tggtgatcat catgctttga ttgaaatttt gctgtaataa
    49321 tgaatcgatc aaagcgtgcc aaagcgaatc tggccgtttt tcgatttgtg ctaacaaaag
    49381 gtggatacat ctgctttgtt gcagataaac cgctgactca tcattgtttt ttgccagctg
    49441 ataaggaagc agccctgttc gctcatttcc agataattgg ttagctaaaa aatctgctgc
    49501 ttcttctggt atctccgaaa acagctgcac caaactctca tacgcagtat caatcaaaat
    49561 ttcacgtggc aattggcttt cactaagcca tttttttagt tggaaagtat aaaaaggact
    49621 tgtctctgct ggaagatagt cttgaatacc cgttgccaga ttagaaatta cttgaacagc
    49681 aaatttaatg agccgccaac tagtttcccc tgttctgcca tagcggtcaa aacgcaaacc
    49741 agtatgttct atttgatgtt cggacaacat atcagcccct gcaggcgtta gctttgcttc
    49801 attttcattt atttcgatcc aaccttgctg cgcaattttt tgcaaaattt gataaaactt
    49861 atcctgcttc aattctggaa aactgttatg gataaacaaa agttcgtggg taaatccata
    49921 aataagtact gaagtcgttc gttttcccac aaggagttgg taaagagaac taatccgcag
    49981 cttgtgtcct gatgaaaata gcgataaaat aaaaacaatc atattattct ccttaagtta
    50041 ggatggttag aaatgtcact actatgtaaa cttgtacctg aacgctgtat tgcatgcggt
    50101 ctttgtcaaa tcgaagcgcc agatattttc gattatgatg aagaaggaat cgttttattt
    50161 gcccaagaac cagaggctca tcaacagttc gtgatgccgg aagaagaaac atatgtaaaa
    50221 aaagcgtatc aaaaatgtcc agttcgagcg attttattag aaaataaaat ataaatataa
    50281 acgaaacttt aaaaacgtac actaagttct ctaaacttga aaataagccg ggatctgttt
    50341 ctcttgttcc gtgaaatccc gacttatttt gaaagattca tttgctatct atagttaatt
    50401 gactagtttt ttcacggacc ctataggacc catttatatt atttcaactt tttcagccag
    50461 ttttccaatg tattgacgat cacagtttgc tggtctgcgt tagaaatttc tgcctctcca
    50521 tctccttttt gctcaccata gctgccaaaa cctccatgat ttccgccatt gattgataca
    50581 tactctgttc tttccggtaa atactgctta gcttcttgat aagcttcctg attcaatact
    50641 ttatcttttg tcgccgtgat cgataaaaca ggaacagctg attctgtcaa tgttcctttt
    50701 ttgtcgggat aacttgccat gaagaatacc ccttttaaat gtttgtctga atgattctta
    50761 ttgacataac gacttgccat tacgccacct aatgaatggc caccgatcac ataatcggaa
    50821 tttggaaaat cttctttgac cttatccgct ttgtttccgc ccatcactgc tagatcaagt
    50881 ggcattttga caatagcgac tgaaaagccg ttttttgcta aatcttgcgc ccaaatacta
    50941 taactttctg gctctacgag agcaccagga taaaaaataa gtaatggaga ctttccatga
    51001 ttctttttct ctccttcaaa aaataagtaa tcttctttgt tttttccttc ttttaccaac
    51061 acttgcgctt ctgacgatgg ttcgtacgta ttttgctgta tatagtaata accggctcca
    51121 ccgagtaatc ctaaaatgat gactaagcca attagtattc gtctaaatat tttcactttc
    51181 tcccaccttt tctatctgag tgaccccttt ttcttaatca agtatgatta taccttaaaa
    51241 aaataagaga gaaatgcaaa ctttccagca tcaaataaaa aaagacggaa tatccatttg
    51301 aatatccgcc ctttttgtat gtttattttt atttagcaca taaacgatac acagcttctg
    51361 cataaatttc cattgcttta tacatactac ttaattccca tcgttcattc gcctggtgca
    51421 taaagtcagg agtatcaggg aacatcgcac cgaatgccac acactggttc atcgtacgag
    51481 caaaagtagc gccgcctgat acaaacggtt cagtcatgtc tccagtaata tcgcgatatg
    51541 tccccaatag atttttcact aactcactgt ctagtggcac gtataatgaa tcaaggaagt
    51601 caaattcttc ataagtcaac ccatattttt ctgctgtttt agttaattta gctactaaat
    51661 catctttctt gaatgtaaca gggattctca tatctacacc gatttttgtt tgttctggtg
    51721 tgatttccaa acttgcaaag ttcatcgtta gctcgccgga ttgttcatct tctgttttgc
    51781 ccacgacagc ttcaccagtt gcattttctt gaactaattt tcctaagaaa ttgattgggc
    51841 caaaatcaaa tacttctgaa agagcaattg ccagacgaga aatagcattc actccttgtg
    51901 ctgcatcttt cgcgtggata cttttaccaa gaacgacgat gccttcacct tgttcttcga
    51961 aatcgaattc gtgttttttc aatgcttctt ttacttcaga aagcttttct ccagagtaag
    52021 gagctgcatc aggaacaaca ttcaacgctc ctccagcttt tacagaaaac tcgttcgttc
    52081 ctggtcctgt caagtaagct tgcaataagc ctttttctgc ataaattaaa ggaaattcag
    52141 catctggcgc aaatccttgc gtaattcctt cttctttttc gttatatttt tctaaacaac
    52201 gccataaatt ttcttcatct gtaccaaaga taaaacgaat acgtgtcttg aattccacgc
    52261 cagcatcaat caaagctttg acggcataca ttgccgcaat agaaggacct ttgtcatctt
    52321 gggatccgcg accaactaac cagccgtctt ttatagttgg atcgaatggt tttgtttccc
    52381 aattattttc gtcccctgct ggaacaacgt ccatatgaca caagataccg aataattcat
    52441 ctccagagcc aatctctgaa taaccgtaat agccttctgg atctttaaat gttctaaatc
    52501 ctagcttttc actgatttcc aatactttat ccaaagcttc aatcactttt ttaccgaagg
    52561 gatgtccttg tcctgaatct gcttcatcta gtacggaagg aattcggatc aattcgctca
    52621 atgattctaa tgcttgttca tgatgttgtt ctgtcacaaa ttttttcatg atattatcct
    52681 cctaatatgt taaaaagggt catgtcaaat tcctccgaca caacctcttc ttctcaaata
    52741 atgacgtttt aagcctgctc catcagcaag cagaatgttc ttaacaaatg atgaaagaaa
    52801 aaacactcac tgaatggagc tattgactta tccacgtgct tatcgatagc cgtttatcgg
    52861 tttcttcatt cttatgatag accttgtacc tgtatttgtc taggtttaga acattgctgc
    52921 tacacctagg atcacacaga tggctacaaa taatatcgca attaatttac cggtaaattt
    52981 ccaccacact gaaacatcga aacgagcaat tgctacagca cccattacga tagcagatgt
    53041 tggtgtaatt aagtttacta aaccagatgc agattgataa gcagtgatta ctaaatcctt
    53101 accgacgccg gcaaattcac ccataggtcc catgattccc attgttgcag cagctaagcc
    53161 ggaagttgaa ggaatcaaga atgacatagg gatatagaaa ataaatgtaa taatgatgaa
    53221 tacacttgaa gataatgaac ttaatccttg ttcgccccaa tgtaagatgg tgtctgtgat
    53281 taatccattg ttcataacca cttggatacc acgagcaaca gctactacga ttgctacacc
    53341 gatcaaatca gaagcaccag ctaaaaattc agagacaaat tcagactctt tcatgccata
    53401 agtgattgct actacgatgg acattactaa gaataacata gtaatttctg ggaagtacca
    53461 gtcacctaat ggaagcatgc ttttacctag aaccgtacct agtaccggaa ggtttgttaa
    53521 ccagtttgtc aatgattcaa aaatcgtgaa attcgcattc aaggttgtcc aaggaatcaa
    53581 actgatgatc atgataccaa aagtaataaa gaacaataca ttaacatgtt tttgtttttt
    53641 cgtgattact tcttgacgac cgattgcttc aatatcgaaa tgcttcatat cttctttacg
    53701 ttggttgtat actaatgatt ttgttggatc tttttcgatc tttgatgcat aacgatacac
    53761 atatacaata gaaatagcta acaaaataac gaacatcaag aatcgaagac caataccttc
    53821 acccatcgaa atcccaacag cttgtgaagc tacgcctgtt gcaaaaggat ttaccgttga
    53881 agctagacag cctacttgag accctactaa tacgattgct acagctacta ctgtatcaaa
    53941 gcctacagcc atcatgactg ggatcagtaa agcatagaat ggaatcgttt cttctgccat
    54001 tccgtaagta gagccgccta atgcaaataa cagcatcaat actggaataa gcattttttc
    54061 ttttcctttg aatttcttca caactgaagc aatcccggca tctagtgccc ctgttttgtt
    54121 gataatacct aaaaatccac caacaaccaa aatgaagaaa gagattggga ttgcagcagg
    54181 tgtttcttta gttagctccc ccgctggtac acctagcatc ccattgattg gcgccatgaa
    54241 aatatcccac agcccttgag gattttgttg tactcgttca tatgatccag cgacgatgtt
    54301 gcctgcatcg tctacactat agtgcccagc tggtataaac caagtaagta cagcgattac
    54361 tgcaataata ataaataaga ccgtatacgc tgaaggcatt tgaaattttt tcttttttgt
    54421 ttgttccatc ttcttcttcc tttcttttcc gttagagaca acaattttgg ctctaatgtt
    54481 attttatcaa aaagagtatc agaacaaaaa taataacacg cattttgtaa aacttttgtt
    54541 caaaaagttc gtttttccct attccctcct cttttttcag gtttttaaag ctttcttctt
    54601 tttcattgta gcgtttcctt ttttttataa acaaacattt tcgtctttta aaaggagaag
    54661 aatttcacat ttgcaaagaa ttgattgatt tttatgcata aatcaaaaaa atactcaaaa
    54721 caaacaaata aaacgtttac attattgcat gtcttgtatt tttttccaaa aaaaaagacc
    54781 aagaaatttc ttgatctttt tgatatttat ttgactgctg tatgacgttg ttcgaactga
    54841 tgttgttttc gacttaacca tggaagaagt tgcgcatgca gcactaaaaa gacagcactc
    54901 acgatgaatc ctttgatcaa gttgaaagga acgatcccaa tcagtacata gtttgctaaa
    54961 gacatgccca gcatctgtgt tgcgttcagc cctaaaaaca ttaagtaaag aggcgtaata
    55021 acgaaataat ttgcgatact cataaaaata gttagagcga tggttcctgt aatgacacct
    55081 aaaaatttgt ttcttccttt atgctgtttc tttacactaa agaaataata aatcgggaat
    55141 gtaaagatcg ttgttgataa gaaactagca gtgtctccta ccaaattatc cggagaaaaa
    55201 cctgtagtca gcagatgtaa tactgaacgc aagaacgctg ttgcaactcc ggccgctgga
    55261 ccgaataaga acatgctgat caataccgag atatcactaa aatcgatctt caaaaaacta
    55321 aaagccggca tgatcggaaa agcaaaatac tgtaaaacta atgcgatcgc agcaaacatc
    55381 gctactgcaa ccattttctg tacacgggtg ttcttcattt gaaatcctcc tgttaggact
    55441 cctcttcagc gaactttttg acgtttctct ccaaatgaaa aaactctatt ttccagggaa
    55501 aatagagtta gagttatttg atcaagcgtc gtcaaataag ctctatcttc tttatccaga
    55561 ctatactgtc ggtatcggaa tttcaccgat tcagctattt ttttgcaaaa taggtcgtgg
    55621 actataacca ccggtcggga atttcaccct gcccctgaag acgaacctta tataattttt
    55681 gtttacatat tcattataca tatctattca aaaaacgcaa catctaacac ttataaaaag
    55741 taagtgttca ttttgagtgg ttcagcaaac tgctgacttc ttttttgttc agttcacggt
    55801 aatccccagg acgtaggcct tgtaaagtca gatctccgta tttttcacgt tttagttttt
    55861 gcacaggaag accaactgct tgaagcatat ttttcacttg atgattacgt ccttcgtgga
    55921 tcgtcaattc cacgatactg tggtttgtct taggatccac tgataaaatt tggaatttag
    55981 ctggagatgt ttttcgtcct tcgatcttta ttccttttgt caaaggtgca agcatcgatt
    56041 ttgtagcgat cccttttacc tttgcaacgt aaactttatc cacttcatgt tttggatgtg
    56101 tcaggcgctg agcaaaatct ccatcatttg tcagtagcaa gatccccgaa gtatcgtaat
    56161 ccagtcgacc tacaggatag atacgttctt ttaccatcgg caaaagatcc gtaacaactt
    56221 ttcttccttt gtcatctgca acagcagaaa taactccttt aggtttgtac aataaatagt
    56281 aaccgtattc ttcttgataa attgggactt catccacttc cacagtgtca tgtttcccaa
    56341 cttttatccc taattccctt acaacttgtc cgtttacttt tacacgtcct gcaaggatga
    56401 attcttctgc ttttcgacga gaagtgatgc cagcgtgggc aattactttc tgcaatctct
    56461 ccattacaat tattctcctt tattcattcc atcaaagaat agatctggtg acacgtcttc
    56521 ttcaagtgaa atttccattt cttgtgtatc aggcagttct tctaatgatt tcaaaccaaa
    56581 ataatctaag aagtagccag ttgttccata taatatcggc cgacctggtc cttctactct
    56641 acctttttct tcaatcagtt tatgggcagc tagacgctgt accgctcctg aaacctgaac
    56701 tcctcgaatc tgctcgattt cagctcttgt gattggctgt ttataagcga tgattgctaa
    56761 ggtttcgacg gcggcttgag ataatgtgtt tgatgtccct tgcgcatatc gcttaagtaa
    56821 gggagccagc tcttttttag tcgttaatac ataatggctt tccgtttcaa aaatatgcaa
    56881 agctgagtgc gagttttgag tgtaagattc ttttaattct ttcactaaac ttgtgacttc
    56941 tgcttcgctg agatttaaca gtccgcttaa ttcttcatta ctgatccctt cctcgccaac
    57001 aataaaaaga agggcttcaa gttcacttaa tttcgtcatg ttcggtatct cctactgctg
    57061 gataaagcat aattgattca tagttttctt tttgttgcgc aatgatctgc ccttttttca
    57121 tcaattctag caaagccata aaagtagtga caacttcact tttagaaggt gactcaaaaa
    57181 aagaatcgaa agtacagcca tgcgcatttc tttttatacg ctcttcgata aataccattc
    57241 gttcttcgat cgttgtgtca tcgggcgtga tcgttttttc cactttttga gaaaatttct
    57301 tacgttctaa gattttctgc atggcacgga tcaaatcgat ttttttgact tgtcctcgct
    57361 tcaacggctt ttcagcatcc gctaacccat cgatgttcat aggttctttt gtgtaataaa
    57421 gacttcgttc ctctgctttt tctgtcagtc tttcagctgc atatttatat ttacggtatt
    57481 ctaaaagctg agtgaccaat tcttctcgag gatcttcttc ccataattct tcatcgtctg
    57541 tgacgaattc ttggacagga agcaatgtct tgcttttgat tgccatcaat gtagcagcca
    57601 tcactaagta ttctcctgca acagctagct cgagtgtttt cattgtatgg atataattca
    57661 tatattgctc cgtgatcgta gcgatcggta tatcatatat atcgatttcc atttgctgaa
    57721 tcagatgaag cagcagatcg agaggaccct cgaatacatc taatttaata ttgatctccg
    57781 tcaactcatt cactctttct tctttttctt tgctagccat tttccaatgt aaggctctgt
    57841 gatttcagtt gcacggatgg agacatccgg gtacttttcc tgtaattcat ctaacatttg
    57901 aaagcccata ttctcaccgc gataagacgg attcaaagaa atgtcatgga tgatcagctg
    57961 atcgttcgtt gcccagtaaa taccaataat cccttgaatg ttggttgaac cttctggcgt
    58021 aaagaaaaaa agttcataat cgtcttcaga ttcgtacgta tcaatctcct tcaacaaaga
    58081 agttttacct gtcagtttct tatggaaact caatagtccc atagcaattt tacggttttc
    58141 tttccggtaa tgtgttaaca tcttctcacc ttcctatgac taagcccttg gaaaatgctg
    58201 cttataaaca tccgtcattc ttttcttcgt gatatgtgta taaatctgag tcgtagagat
    58261 atccgcgtga cctaacaatt cctgtactgt ccgcaaatca gctccatttt ctagcagatg
    58321 tgtggcaaag ctatgccgca gcgtatgtgg tgttacttct ttgtaaatcc cagcctctcg
    58381 tactaactgc ttcagatttt tccagattcc ttgccgagaa agacctttcc catgattatt
    58441 gacaaataca tgcatttcag atgcgtcctt tacaagtaat ggacgtgctt cgtctaagta
    58501 gcgttccagc cattggatcg cataatcacc taaaggaatg atccgctctt tatctccttt
    58561 tccaactgtc tgtaccagtc caagagaaag atgaagatct cccaatttca atccaatcaa
    58621 ttcactgacg cgcatcccag tagcatacat gacttccaag attgcacgat ctcttatccc
    58681 taacgttttt gtcgtatcag gtgcttcaat cagcttttct acttctgtca gagtcaatgt
    58741 acttggtaat ttctgtgctt tcttcggcgt atcgatatgt tgcatcggat catgatcagt
    58801 gatacgctct tgccgtaaaa actgatggaa tcttctgaga cttgagatca tccgagtaac
    58861 agttgctgtc gctttttttt cttcatgcaa cttctccaga aaacggatga ctaaaaagcg
    58921 atcgacatcc tgccatgaag caattttctg ctcctgtaga aaatgcaagt actgttccaa
    58981 atcacgttca tagctttgtc gtgtattggc ggacaatccc cgttcaatat tcaagtagtg
    59041 gagataatcg ttgatttgct ctttcataat aatccccctc atgcctctct atcatatcag
    59101 ataccgcttc attctgatag tctttttcca taatctgagc aaagaaaaag ccgtctagac
    59161 actcagacgg actggtttct tttttctttt tcatagcatt cctgacagat tccatggaac
    59221 gttagccgat gatcttttac taaaaaatga taacgttctt ctactacttg ctccacttca
    59281 agtaaaagat cctcttctac ttcctcgatc gtgccgcagt tcaagcatag taaatgatga
    59341 tggaaatgtt tggcgccttc ttttctcaaa tcataacggg ctaatccatc attaaaactt
    59401 actttatcga caattttcaa ttctgtcaag atttcaaggg ttcgataaac agtagcaagt
    59461 ccaatttccg gactcttttt tttcactaaa aagaagattt cttctgcaga taaatgctct
    59521 ttctcatttt ccaaaagcac aagcagagta gcttctcttt gcggtgtcag cttaaacccc
    59581 gcttcatgta gctgctgttt tgtttttttc atcgccgaaa tagcattcat gaccatttac
    59641 tcaaccccaa caatttataa taattacaaa taaaaaaaga tataacacta tgttaattaa
    59701 aattattata agctagttaa gaaaaacatg caagggattc tcaattagat tttttgacta
    59761 gttttgtcac accgttttct tgtgtaatca atccttgttt caaaagatta cctaatgcgc
    59821 gcttaaatgc tcctttgctg ataccaaaag ctttcatgat ttcttctgga tccgatttat
    59881 ctgtaaagtt gatttgctga tctgcagaac gttctaaaat ggttaaaatc atttgtgcat
    59941 catctgatat agcttcatat gctcttggct tcaatgaaag attcagtaca ccgtctggtc
    60001 ggacaccaat cactcgacct tttatctgct cgcctagacg aggttcatta tatcgttcag
    60061 aaggatggat aaacccaaga tagaaatctt ccgttaaaac gtacgtacct gttaatttca
    60121 agcggtatgc tgttcctatc acatctttgt ttttcatttc ttctgttcca tatttgctta
    60181 aagatttgaa tattttttca tctgctaaac tgccccaaat acggtctttt gcatcaactc
    60241 taagggaaat catcaagcga tccccttttt taggccatag ctctgtcatt gtcggcaatt
    60301 catcaagaga tacgacaaag tctttatctg gaagaccgat atcgacaaac gcacctaaat
    60361 cttttcgtgt agcagtaacg attccaaaag cgtaatgacc gatacggctt ttaggaattt
    60421 gtgtagtgat ccgattttca tgtttttgat tttggtaacc gaatccttca actgcttcac
    60481 cgattttgtg ttcaccttct gatttgctta agccaaaggt ttgtccgttt ttttggacaa
    60541 agtagttttt ttcattttca tcaataataa tacctgtaaa tatagttgct aataaatcgt
    60601 tcattttttt ctccctctat agtgaaaggt tttcttaata catttctttg ctatcataac
    60661 ataatgttat ctacttgaat agctgagcct tggtttggaa aggtgaaaaa aggagtatga
    60721 caacagttgt gtcatactcc cctttttcga ataaacggtg ttaaacggca ggctcacagc
    60781 ctctttcatt tgcctaaaat taattgctcc catttcctcc gaaccatcca gttttacagt
    60841 tgatggttac gatgtaaaat ggcaaagcac tttctccaat gcctttcatc aatgcagatt
    60901 ttgttgaagt ggcatataga ttataatagc cttccccatt ttcgattctg gctttttcta
    60961 atctgtaacc tccttcaacg atatatccac gttggataca tgtagcaatc actttgcttt
    61021 ggacacctgg tgcccacgtc catgctggat tagatgcatc ggcaatatca cttgctctgg
    61081 cagatttgat tggctgattc gctgagtttg aagaggaagg ggtcgaggat gccgcttctt
    61141 gctgtgctgc ttgtgcgacc gcagcttgtt gagcggcttg agctgcagct gctgcttgag
    61201 cggcttgttt tgcttgttct tcagctgctt ttttcgcttc ttctgctttt ttcttttcct
    61261 cttcttcttt tttctcttta tcagtgagca tcttatctac ttgtttcaat tgttcattca
    61321 actgagcaga tagatctgta tttttcactt tttctactgc ttcttttgcc gtcttgtatt
    61381 ggtcgcgtgt tgctgaatcc actacttttc cgtctttaaa gactatagct gttttttctt
    61441 tagctgtttg caattcagca tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt
    61501 ttccaaaatc tgtgttatcc gtttcgattg cttggatctc tttatctgct ttcaacttga
    61561 tgtcatctgc tagatgatca tcgacgatgg caggtgctat gaaccactca ttcacttttt
    61621 ggatatctcg gatcttagat tggatatctt ggtaagttgt ttctaactct gaatatcctt
    61681 tttcattctt aagactggat aaatcatttt ttagttttga taaatcttga tgaatcatat
    61741 ctgcacgaat aaattgatgg tgatcatctg tataaaaggc agcaagtttc tcatgaagtt
    61801 cttccatttg ttgctgtctt tttgcttctg ctgctacttc ggcttctact ttttgatgat
    61861 ggtcataata agcccagctt cctccaccga gtagaagtac tgctgctaaa acgagataag
    61921 gtactttttt tgattttttc ttttgcgctt tatctggatc tgttttttct aagtttgatt
    61981 cttgatgtgt tttttcagta tctggcacga ttgttattga tttcgcttct gcttcaaaca
    62041 gcttaggctg gttctcttct ttttctattt ttgcttcaag atcagtaccc tctttgctta
    62101 cagattgatt ttcagcatcg gattcactat ttatttcatc gggctctgtt gactttttgt
    62161 ctgtgttttc gagtatttct tttttctctg cttccggcag tttattcgtt ttttcctttt
    62221 ctaattgttt ttcagtaaat acttcagttt tttctgtgat atccgttttt acgtctttta
    62281 tttttactgt ttctttacct gtggcgtttt ctctatcaga gtctttttca gtttcttgtt
    62341 ccttttctgg ctctactttg ttttcctgtg caattttttc actgatcatt ttgagcagct
    62401 ctttcgcgtg ctcttctttt gtatcttcac gatgtgcttt gatataagcg gatagtatcg
    62461 aattttcttc ctcattatcg acgatttctt tcagttcagc tactcttctt ttttgttttg
    62521 attcaagcgt ttcttcttga ttctttcctg attcttccga agtctcatga acagttgctt
    62581 tcttctgttc atttttctct atattagtta cttgatcgtg agcaggtggt tcttcgtctt
    62641 ctggttctgc tttttcgaat aattccatta cagattctag aggtaaatct ttcaattccg
    62701 accattcgat tggatcgttt aattctgtat caggaaagac tgcatcttca gttgatttat
    62761 cctgcatagc tgcatctgta tttgtttctt caattgattc ttttatttgc tgatcttctg
    62821 cagttatttg atgaccgcag tctggacaga tagtattctg ctccattttt ttcgatccgc
    62881 aatttggaca ttttttcatc acttagcgat cggctccttt actaggttta cgctaccatt
    62941 taatctatca tattctatac gtcacgccaa tagcttattt ttaggattta cttaaaaata
    63001 atgaatgaat ttccttccta tagaaaaaaa agcttgagac tgcgtagtct caagcttttt
    63061 ttcttttaaa ataaaccaaa cagttttact gatttctctt ctggtcaata gtccacgctt
    63121 attctaagaa atcttttaat tgttttgaac gagaaggatg gcgcaatttt cttaatgctt
    63181 tagcctcgat ctgacgaata cgttcccgag taacaccaaa tactttccct acttcttcca
    63241 atgttctcgt ccggccatca tctaaaccaa aacgtaagcg aaggacattc tcttcccgat
    63301 cagtcaatgt atctagtaca tcttccaatt gttcttttag taactcataa gctgcgtgtt
    63361 ctgctggact tgttgcttcc tggtcttcta tgaaatcacc taagtgagag tcatcttctt
    63421 caccgattgg cgtttcaagg gaaacaggtt cttgcgcgat tttcaagatt tctcggactt
    63481 tttctgttgg aagatccatt tccgcaccga tttcttctgg cgtaggttct cttccaaggt
    63541 cttgcagcaa ttgacgttgg atacgtatca atttattaat tgtttcaacc atatgaactg
    63601 gaatacggat cgtacgtgct tggtcagcga ttgctcgagt gatggcttga cgaatccacc
    63661 atgtagcata ggttgaaaac ttaaatcctt tgcggtagtc aaatttttca actgccttca
    63721 tcagtcccat gtttccttct tggatcaagt caaggaactg catgcctcgt cctacataac
    63781 gtttagcgat acttactact aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt
    63841 ggtcgccttc ttcgattttc aatgctaacg ctacttcttc ttccgcagtt aaaagggaga
    63901 cacggccaat ttctttcaga tacatacgaa ccgggtcatt gatttttaca ccggttggcg
    63961 cagaaagatc ttctgttttt gcttgttcag cttttttctc agcactcttc aagctgtgaa
    64021 ttgatggatc accattttca tccaccacac tgatacctgc gtcttcaatc ttttggatca
    64081 atttgtccat ttcatctgca ttcaaagtga atggtgtagc aagctgattg gataaatcat
    64141 catatacaac ttgtcgtttg ggtttgtttt ctttgatgaa agctgctact gctgcttcat
    64201 acgttttttt atctgttcca tttgccataa aagaaggccc cccttgagta gctatttggc
    64261 ttgttttaac tgtttcgtta aattgatgat ttcaatcgtc aattcaagct caagctgttt
    64321 gtttccagtt cgtctggctt cttgctgcat ttgttttttt aatgttattt catcagctaa
    64381 gccagatttg ctgatcacat ctagcaagtc ttggatttct cgtggcgaac tttcttctgc
    64441 caccggtaaa tattcgattt ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc
    64501 taaaaactct gcaaggttga attcataatg ttgtgagaca tatgcatcga ataaaaaata
    64561 aacttctgca tatgcatcat gaataaactg aaattctcgt tcttttaaaa tattacggac
    64621 agaaacttct ttaaatgcac gatataatag aatctgctct gccttttcta cttgcgtcaa
    64681 aggtctcttt ttccgtgcag tttcttgtct cgtaacctga gtgtgttgac tttcctgagg
    64741 ttcttgacga ttttgctgat ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc
    64801 tctgccgatc tgaaactctg ttgacagttg agatagatac atatcttgtt cgattggtga
    64861 aacaactcgg ctaagttcaa ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt
    64921 atctaaattt ttcccttgtt ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc
    64981 atgagccact aattcttgga aagatgcttc tccatactta cggagatatt catcaggatc
    65041 aagcttttct ggaacgacta cgacagaaag tgctaatcga ctgttttgcc gaagcaattc
    65101 aatccctcga ttggttgctt caaaacctgc gttgtctccg tcatagcaaa agaccaattc
    65161 tttcgtcagc ttttccattt gttggatctg ttgagctgtt agacttgtcc ccatagaagc
    65221 taatccagtt ttgataccag cctgccatgc agcaatcaca tccataaacc cttcgaacaa
    65281 caaaatttct ccgtttttcc ggatatcgct tcttgcttta tcaagattaa ataaaacttg
    65341 tcttttattg aacagttctg tttctggact attcaagtat ttgggctgat ctttttcctg
    65401 ccctttttca ggtgcaagcc agcgtcctga aaaaccgatc gtctggcctt gagaattacg
    65461 aagtggaaac atcactcttt gataaaaacg atcagacata cggccatcat catgcgtgac
    65521 aaacaatcca gagcgttcta acaattcatt tgaaattgat tcattcttga atacttgctg
    65581 aagaaaagaa cgctcattag gtgcaaaacc tatgttgaat tcttcaatta attctaaaga
    65641 caacccacga ctttgcaaat attctaaagc tggctgtcca gcttttgtat gcaatagcat
    65701 gtgatgatag atttccgctg ctttttcatg agcagcaatc agtttccccg tagcagattc
    65761 aggatttcgg acagctatag tacgctgacg ccattgatct tctaacggta tctgttcgaa
    65821 gtctgccact tttaccactg cttctggaaa acttagacct tctagttctt gaagaaaagt
    65881 aaacacgttt ccgcctttac cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac
    65941 agaaaaagac ggtgtacgtt cttcatgaaa aggacacagc cccaagtaat ttttaccaga
    66001 ctttttcaac tggacatatt gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt
    66061 ttcgataacc tcttgaggaa tccgttgagc cattttttca ccccctgctc tattgatatc
    66121 ttttgggttt aacagaagat atttcaggag aataaccggt aaaaaatcgc actaactgaa
    66181 atcagtgcga ttttaaagga cacaattata catgtttgat tgtatcatat ttcattgctt
    66241 tttcaagtat aacacatttc tatctcatta agcatcaatt tgctactctt ttcagtaact
    66301 gattttgaat atcagccttc cagtacaata ctcgaagaac aatatttacg agcatgaata
    66361 caaagaagta tcctacgctc tctcccaacc ctaagtttac ttccaaaatt ccatggaaca
    66421 gcattgcagc tacatatatc cctacgatcg tcgttactgc taggaagatg cgcaatgggt
    66481 tgaagggata acaagctttt acaactgcca tgcaactgat cccaatcagc aagtaataca
    66541 tcaaagtagt agtttcagca tttccccatg cgaatgtttt tccaagtaga tacaccgcaa
    66601 tgatattcac tactacaagc aaggcatttg gtaaagcatt aataagtgct gtaggcaaat
    66661 aacggtaagt gatttttctt ttatcttgtt caaaagataa aaagaaggaa ggataccctt
    66721 caatcgctaa atcaatcagc gtgatttgga tagggataaa cggaaatcca attgctgtaa
    66781 cggcacatat aatcgataaa ataaatgaat aaatcgtttt gataaaaaag atgcctgaga
    66841 ctttcgtcac attgttgacc actcggcgtc cttcaaacag gacttctggc aatgtagtaa
    66901 aatcagagtc caacaatacc agattcgcaa tctgacgtgt tgctccatca ccttctgcca
    66961 tagcaatgct gcaatctgct tctcttaacg ccaaaacatc attcacaccg tcgcctgtca
    67021 ttgcaaccgt tctgccgctt tctttcaatt catttaccag caatttcttt tgctgcggac
    67081 tcactcgtcc aaaaaccgta tagcgatgag cagcttctcg cacttccatt tcttcagtaa
    67141 tttgagaaag atcgacatac gcatcataat tcggtaatcc tgcacgtcta gcgatattgg
    67201 agacagtaaa aggattgtct ccagaaatca ctttcagatc cacaccttcc tcatgaagat
    67261 aagctagtgt ttcttgagcg ttttttcgga ttggatcatc gatttcaaac agagcaaccg
    67321 gctcaacaaa tgctggtttt tcttcgttca attgctgatt tggagcgatc cccaacataa
    67381 gtacgcgata gccattcgct tgggctgttt ttagactgtc cggaagccga tcttcttcca
    67441 ccaaacgttc aggcgctcct acgtacacga ctcccagttc tggaaattcc atcgcgcccc
    67501 atttgcgatc agaagaaaat gcgactttgt ttgtcacatc ataccgatta gaaagctcaa
    67561 aataatctct catagcccgc atagtgatgt tgttatcact gctttctgcc agatacgtcc
    67621 ccataatttc tgggatggct ttctcgtaca gcggatgtag tgcttctacc cgctggatac
    67681 tcattttccc ttcagtaatg gtccctgttt tatccaagca taatgtatcg acatgggcca
    67741 gcgtttcaat tgaatacatg tcttgaacaa ggattcgttt ttttgccagt ttggttaccc
    67801 ctgtcgttaa tgcaatactt attagcaata ccaaaccctt tggaagcatg cctaacaaag
    67861 ctgctgcaga tgcaataaca gaaattttga tagaagcatc gcggatgaac agtgcttcca
    67921 aaaatagtat gatccccaaa ggaataatca cgaaactagt gaatttggat actttgcgaa
    67981 tcgaatctac aagttcagaa cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga
    68041 cagcataatt atctgcacct actcggataa cttcagccat gacagaaccg cttgtcaaaa
    68101 agcttccaga aagcaattca gctccagttt ctttgacaat caaatcagat tcgcctgtca
    68161 aaagagcttc atttgcttct gcccgtccag atataacctg taaatcggct ggtatctgtt
    68221 ctcctgctcc gatttttacc aaatctccta ataccagctc tttagtagaa atcgattgtt
    68281 ctttaccgtc ccggatcact agtacttctg attgggaaac aatcgataat ttccgtacaa
    68341 gatttcttgc atgtatttct tgataaattc caataacgat attgacgaca ataattgcta
    68401 aatacgccat gttggaatac gcacctacaa ataacagaca aatcccaata ccaaagttca
    68461 gaaaattgaa taacgtcaat atattatcgc tgaatatttg ttttgtactt tttgatgcat
    68521 cttcctggta gtcattttgc tggccagaag ccattttctg aactacctct tgctgtgtaa
    68581 gtccattcat tttctttttc tccattttcc gccaactttc aaaaataatc acgtgtttaa
    68641 gtctagcgtg ttccaagatt aaaaacaact tgtttttata aaatttatag aaaaacacac
    68701 gaatgatttt acaaaaaaga tgccaacctt tttgtttgaa tcacccgtgt gctgttttta
    68761 ttatcaatct atctttactt atattgttcc tccaaacggc tcatccacca acctagtgct
    68821 gtaccggcta taaaaagaac aggacaagct agtaccccag caactcccaa tcccgcgtaa
    68881 tcggtaggaa tgatcataat cgttgaagca aaaactacac ctaaaatgaa atggaatagc
    68941 ttgctatatg cctttgagaa aatgtaatcc atgatttttg acaatccaag aacacaaaga
    69001 atcccaccaa ttccgatcgg cacaatcact tcaagtcgta cttctttaaa tccgtcagcc
    69061 atcgctttat acaatcccat atacaccaaa aaattagaag ggctcaatcc tgggacgatc
    69121 atacccaaac caatcaatcc gccggcaatc agccatgttc caaaattctg atcgacttgc
    69181 ccgtcaaata attgagcgcc gtataataaa aacaaataag ccagaacaaa agtaacaacc
    69241 atgatgatca catcagaagt atctcttcct tttttccctg cttctttcca taacgcagga
    69301 acagttccag cgatacatcc gacaaaaaac cataatatcg ttgtttcata cgcacctaat
    69361 aaaaaactga ctaaaaaaga aagtagaaag atgcctgtca ctccgcctag accaacaggc
    69421 agaaaaaaca aaacattttc tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc
    69481 cgctcataca tgccaaagac agctgctaat gcaccaccgc tgactcctgg caagataaat
    69541 cctgaaccga taaacattcc cttcacaaac cgtaacatcc agtctttggt tgtgtacccc
    69601 acttcttgat tttccatttt ttgccttttt tccactattt gcgtactcct tctttcattt
    69661 aattttattc atgattgaac atttcctgct attttactta aagacttttt cataggtctc
    69721 tattattttt tcattcattt tgacaatatc aggataaaac atgttacctc cattatgata
    69781 gagatagccg ccattgtaaa gcaaggactg gatccgccaa tatcggtatt gtttcccttc
    69841 ctcatttcct aaaagtgggc tcagcacatc tttagaatac gcttctgcta attctactgt
    69901 gttttctccg ccatgatttt ttacataggc aatataatct ttcccaaaat tatatgcttg
    69961 gacagctgtc cataaatcgc atccttgttt ctttgcttct actatcattt ccgccaaata
    70021 tttgacacct tgatcaatac tgtcagctgg attatcaatg atctcagctt ctccatgcag
    70081 actttccgag ctctgcatga gatctgtccc ttttcctttt gattcggtca atattatcgc
    70141 cttgatcaaa ttctcgtagt tttccatatg ataatttttt gcagctgttg ttatttctat
    70201 ttgatagctt tccactcgtt ttaccgaacg ataagttctt cctcataaaa acaacaccgc
    70261 accaataacc gtcagaatga tcatgtaaaa aagaatacgg ataatagata atcgttttcg
    70321 cttcatttca tcacctagtt attttgacat ctttttttat cttactattt ctttatcttc
    70381 ttggctattt cttccttgat ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt
    70441 ttttgacatc ttacgaaaat gatcgttgtt tttcagtaaa aacaattgcc caatgactga
    70501 tataatcgtt atactttaca cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa
    70561 aatgccagat gggagcgaaa tttactatga aaagagcggt caaggcttcc ctctcttttt
    70621 actgcatggc aatgacggca gcggccgctt tttttcagaa caggtccctg tactcgaacg
    70681 ctactatacc gtgtatctag tagatagccg aggacatggt cgttcgacaa acgaagcatc
    70741 tatgttgaat tttcagttaa tggccgagga tctgaatacg atcatgttgc tggaaaaaat
    70801 cgatcaggca gattttcttg gatttagtga tggagcaaat ctagcactgg tatttgccag
    70861 cagcttccct aaaaaagttc atcgattgat tttaaattct ggaaacacgc ttgttaaagg
    70921 cgttcgtttt tctgccagag tcatcagtaa tattcattat gcatgggtat ggttactctc
    70981 tttatttcgc ccaagtcttc gcaaaaatct tttagttatc aaacttttgc tgcatgacat
    71041 cgggcttaca gaaaatgatt taaaaaaaat caactccccc actttgatca tcgtgggaaa
    71101 aaaagatgta atcaagctaa aacattcttt gtatatagct aaaactatac caaaagcttc
    71161 ctttgtgctt gtgaaagaac aaggacatga attagctaga aaagatcctg aacgcttcaa
    71221 tcgagaagtt cttcaatttc tttctgaaac ataactatta ggaatagaag gtgatttatt
    71281 tgttaaaaaa taccatacaa tggctgaaaa accacctagg actgttcaaa acaatctttt
    71341 tgatttctgt cattgtcatt attgtcggtg aattgatgtc catcggtaaa actctatcta
    71401 tccagcaatt aggagaaacg tttgccacta tacctgtatg gaaaagcgga ctaatgctat
    71461 tgattggtct tatctctgtg ttacctatgg ttggttatga tattattttg aatcggatgc
    71521 ttgaacaaaa gcagaatcct cgttacctgt ttgagacaag ctggctgatc aatacgatca
    71581 acaatattgc cggtttcggc gggtttgtta gtgtggggct tcgctctgaa ctatatggac
    71641 aaaagaaaga gagcagaaac gtgatccaag cattatcgaa agttttcttg tttttgatgg
    71701 ctggactttc tatttacagt ctgctttctt ttttacttgt agtcttcacg ccagttgctc
    71761 cttttttgaa gcaatattgg atatggttga tcggcggcgg tctatatttt cctgtcgtct
    71821 ttttatttac tactttcaaa aagaaaggat tccttggtgg tacctcactt aaaactcaag
    71881 gacagctatt gctagtttca ttgttggaat ggaccggtgt cctgtttagt ttcctttctg
    71941 ttggttattt aatggaaatc cggattgatc tgtggcaaac aattccatta ttcattgctg
    72001 cttcagtgat aggaatcgtg tctatgattc caggagaaat tgggagtttc gatgtaatga
    72061 tgattatcgg gttgtccgct attggtgtgc caagagaaat agtcgttgtg tggatcttgc
    72121 tctatcgcct gttctactac atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa
    72181 atatcggttc cacttttgat cagcgttatt ctggtatccc taaacaatta gcaacagaaa
    72241 ttgcgcataa aatcgtggtt gtcttattat acttttctgg aatcatgcta gtactttctg
    72301 ccactatccc gcaagctttt acagagtttc gctggcttca tagtttgaac ccattgaaat
    72361 ttcattttat tatccaattt cctagtatac tattgggatt tcttttaatc attatgggca
    72421 gagggatcgc agcacgtgtg aaacgagcat atttgcctac aatcttcttg attgttctag
    72481 ctttacttta tgttttatta agtgatttta gtttcacacc ggtggttttc ttgtctattc
    72541 tgcttctgat cattcttgct tctaaaaacg aattatttcg ggaacaattg atctattcat
    72601 gggagtggcg tacgatcgat ggaatcatta ttggtgcact gtccttgctt tacatcgtta
    72661 ttggggtata caatctgccg gatttccctc atcggcgtca ccattttatt tcctttttct
    72721 tatttccttc tgaaaagatt tggttttcgg gattgttggc catcatcgct gttagtttca
    72781 tgatcgttct atttgttcat ttccttcaag gagaaaagaa acagattggc gaagccttta
    72841 atgaagaaaa ggccctgaag atccttacaa cttatggagg aaactccgat agtcaattga
    72901 tcttcttaaa agataagcgg atgtttgcct atgaaaaaga cggtgaacca actgttcttc
    72961 tccaattcgc ttgcttcaat aataaatgta ttgtaatggg tgatccttct ggtaagaaag
    73021 aagattttcc agaagcaatc gaagctttta tcgaagaaac agatcgacta tgctatttac
    73081 cagttttcta cgaaacaagc gaagagattg ttatgatttt gcatgaattc ggctacgatt
    73141 tcattaagat gggggaagaa gcttatgtag acctgaatag ttttacaact tcagggaaaa
    73201 aaatgaaggg cactcgtgct gtcttgaatc ggatcgaacg agaaggattt acatttgatg
    73261 tcttacaacc accattttct actgagcaaa tgagtacttt caaaaatatc tctgataatt
    73321 ggttgggttc acgaaaggaa aaagggtttt ctttaggctt tttctctgaa gattatctac
    73381 aaagagcgcc tattgcagtt gtcaaaaata cccaagaaga aattgtcgct tttgcaacga
    73441 tcatgcctac ttacacgaac aataaagtag gaacgattga tctgatgcga tatgattcta
    73501 aaaaagcacc ttctggcagt atggatttct tgttcttgca tttgtttgcc tatatgaaag
    73561 aagaacatat ccaatggttc aatttaggga tggcccctct ttcaaatgtc ggaacttcca
    73621 gaaagagttt tttacaggaa aggattgcat acttggttta cgagtttggt tctcattttt
    73681 attctttcca tggattaaga gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt
    73741 atactctata ttctagagat agttggattg catatgttat gattgctttg ctgattattg
    73801 ataatgcgcc agttgagcag acacaaggta gaataagtgg tttgaaaaaa tggatacgaa
    73861 gaaagtatta aaaactgcat accgatagat gaacactctc atgatgaata aaagagactg
    73921 aagtaaaacg aaagttcatg cttctacaat ctcttcttat acaattgaag aacaaagagc
    73981 ggtccaaaca gaaatcctgt ttggaccgct cttttatttt aaacattttt cattaccaaa
    74041 atattcgagt cgcgtttctt atgcttcgat ctctgtaacg atacctgaac ctactgtccg
    74101 tccgccttca cgaattgaga atgtcgttcc gttttcaaca gcgattggat ggatcaagtc
    74161 aacatcaatc gtcacgttgt caccaggcat gaccatttct gtatcttctg gcaatgtgat
    74221 tgttcctgta acatcagttg tgcggaagta gaactgtgga cgatagttgt tgaagaatgg
    74281 agtatgacgt ccaccttctt cttttgtcaa cacgtacact tctgctttga attttgtatg
    74341 tggagtaatg gaacctggtt tagcaagtac ttgtccacgt tcgatatcgt cacgttggat
    74401 cccccgtaac aacacgccta cgttatcccc agcttccccg taatctaacg ttttacggaa
    74461 catttctaca cctgttacaa ctgctttttg tgtttcaggt ttgatcccta cgatctctac
    74521 ctcatcaccg acacgaacag caccacgatc aatacgacct gaggcaacag ttcctcgacc
    74581 tgtaattgag aagacgtctt ccactggcaa tagtaatggt ttatctgtat cacgttctgg
    74641 tgttgggata tattcatcta ctgtatccat caattccata atagcagctt cagcatctgg
    74701 atcgccttgc aatgctttta atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa
    74761 accatattcg ctcaataact cgcgaacttc catttctacc aaatcgatca attcttcatc
    74821 atcgactaaa tcaactttgt tcaagaaaac aatcaggtat tttacaccaa cttggcgtga
    74881 caataaaatg tgttcacgtg tttgaggcat cggaccgtca gttgcagaaa caactaagat
    74941 tgcaccatcc atttgtgctg cccctgtaat catgtttttc acatagtccg cgtgtcctgg
    75001 tgcatcaata tgcgcgtagt ggcgtttttc tgtttcatat tctacgtgtg cggtattgat
    75061 cgtaatccca cgttcacgtt cttctggagc tgcatcaata ctagcatagt cttgaggatt
    75121 tgctaggcct tttttgccta atactgtagt gatcgcagca gttaaagtag ttttcccatg
    75181 gtcaacgtgt ccaatcgtac caatgttaac gtgtggttta cttctgtcat aatgttcttt
    75241 tgccataatt tacaaacctc ctaaataata tagatcactg aaaaatttca gctctcacct
    75301 ttttattata gtcttttttt gactaaatca aaaagaatac gtccgactta ctttttgtaa
    75361 gattcgaact aagcttatta tacgtcaata ttggtcaaag tcaactaaga gcactcgtct
    75421 gtcttatacg cgaaaaaagt accttttata aataaaaaaa tgtgtttttt gagaaaaact
    75481 tcaagatagt ccttaacttt cttttgaagc aataagaaca aaatgaggaa aagtttaaaa
    75541 ttcagctgtt gccaacaact ttcaatagac gaaatcttct atttcgttaa taataaggaa
    75601 agaggtgaag tacatgaaaa aattaattgg cgtacttgtc gttttaggca tatttctcgg
    75661 cggaagtttt gcagcttatc attattttta cggcggcgaa gcttattata caaaaatcac
    75721 gacgaatggc gaaatgtcag ctacccaagc agataacgga gagaaattca cgacttatac
    75781 ctacaagcaa gcggcttata ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga
    75841 aagagaacat ccattaaaga tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg
    75901 tgtattaagc tgggaagaaa taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat
    75961 cgataattag taaatggtct taaaatttta ttagataaaa
//
LOCUS       DAZKNH010000002        70744 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000002 DAZKNH010000000
VERSION     DAZKNH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 70744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.002"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            126..644
                     /locus_tag="ACG8MG_000071"
     CDS             126..644
                     /locus_tag="ACG8MG_000071"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0374152.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            772..1455
                     /locus_tag="ACG8MG_000072"
     CDS             772..1455
                     /locus_tag="ACG8MG_000072"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFQ0374153.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1764..1837
                     /locus_tag="ACG8MG_000073"
     tRNA            1764..1837
                     /locus_tag="ACG8MG_000073"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1798..1800,aa:Arg,seq:tct)
     gene            1996..2355
                     /locus_tag="ACG8MG_000074"
     CDS             1996..2355
                     /locus_tag="ACG8MG_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFQ0374154.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2478..4109
                     /locus_tag="ACG8MG_000075"
     CDS             2478..4109
                     /locus_tag="ACG8MG_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFQ0374155.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4171..4812)
                     /locus_tag="ACG8MG_000076"
     CDS             complement(4171..4812)
                     /locus_tag="ACG8MG_000076"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0374156.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4998..5074
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5094..5402
                     /gene="rplU"
                     /locus_tag="ACG8MG_000077"
     CDS             5094..5402
                     /gene="rplU"
                     /locus_tag="ACG8MG_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFQ0374157.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5429..5773
                     /locus_tag="ACG8MG_000078"
     CDS             5429..5773
                     /locus_tag="ACG8MG_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFQ0374158.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5786..6079
                     /gene="rpmA"
                     /locus_tag="ACG8MG_000079"
     CDS             5786..6079
                     /gene="rpmA"
                     /locus_tag="ACG8MG_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFQ0374159.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6513..9320
                     /locus_tag="ACG8MG_000080"
     CDS             6513..9320
                     /locus_tag="ACG8MG_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0374160.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9511..10002
                     /locus_tag="ACG8MG_000081"
     CDS             9511..10002
                     /locus_tag="ACG8MG_000081"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0374161.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10105..11094
                     /locus_tag="ACG8MG_000082"
     CDS             10105..11094
                     /locus_tag="ACG8MG_000082"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0374162.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11127..11930
                     /locus_tag="ACG8MG_000083"
     CDS             11127..11930
                     /locus_tag="ACG8MG_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFQ0374163.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11951..12865
                     /locus_tag="ACG8MG_000084"
     CDS             11951..12865
                     /locus_tag="ACG8MG_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0374164.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12948..13322
                     /locus_tag="ACG8MG_000085"
     CDS             12948..13322
                     /locus_tag="ACG8MG_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFQ0374165.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13461..13706
                     /locus_tag="ACG8MG_000086"
     CDS             13461..13706
                     /locus_tag="ACG8MG_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0374166.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13878..14942
                     /locus_tag="ACG8MG_000087"
     CDS             13878..14942
                     /locus_tag="ACG8MG_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFQ0374167.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15188..15613
                     /locus_tag="ACG8MG_000088"
     CDS             15188..15613
                     /locus_tag="ACG8MG_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0374168.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15606..16055
                     /gene="nusB"
                     /locus_tag="ACG8MG_000089"
     CDS             15606..16055
                     /gene="nusB"
                     /locus_tag="ACG8MG_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFQ0374169.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16152..17006
                     /locus_tag="ACG8MG_000090"
     CDS             16152..17006
                     /locus_tag="ACG8MG_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFQ0374170.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            17007..18344
                     /gene="xseA"
                     /locus_tag="ACG8MG_000091"
     CDS             17007..18344
                     /gene="xseA"
                     /locus_tag="ACG8MG_000091"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFQ0374171.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18349..18573
                     /locus_tag="ACG8MG_000092"
     CDS             18349..18573
                     /locus_tag="ACG8MG_000092"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFQ0374172.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18575..19465
                     /locus_tag="ACG8MG_000093"
     CDS             18575..19465
                     /locus_tag="ACG8MG_000093"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0374173.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19474..20292
                     /locus_tag="ACG8MG_000094"
     CDS             19474..20292
                     /locus_tag="ACG8MG_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFQ0374174.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20399..20848
                     /locus_tag="ACG8MG_000095"
     CDS             20399..20848
                     /locus_tag="ACG8MG_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0374175.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20866..22548
                     /gene="recN"
                     /locus_tag="ACG8MG_000096"
     CDS             20866..22548
                     /gene="recN"
                     /locus_tag="ACG8MG_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFQ0374176.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22603..22719)
                     /locus_tag="ACG8MG_000097"
     CDS             complement(22603..22719)
                     /locus_tag="ACG8MG_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFQ0374177.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22792..23748)
                     /locus_tag="ACG8MG_000098"
     CDS             complement(22792..23748)
                     /locus_tag="ACG8MG_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0374178.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23919..24287
                     /locus_tag="ACG8MG_000099"
     CDS             23919..24287
                     /locus_tag="ACG8MG_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFQ0374179.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24457..24888
                     /gene="mraZ"
                     /locus_tag="ACG8MG_000100"
     CDS             24457..24888
                     /gene="mraZ"
                     /locus_tag="ACG8MG_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFQ0374180.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24909..25868
                     /gene="rsmH"
                     /locus_tag="ACG8MG_000101"
     CDS             24909..25868
                     /gene="rsmH"
                     /locus_tag="ACG8MG_000101"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFQ0374181.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25873..26274
                     /gene="ftsL"
                     /locus_tag="ACG8MG_000102"
     CDS             25873..26274
                     /gene="ftsL"
                     /locus_tag="ACG8MG_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFQ0374182.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26275..28467
                     /locus_tag="ACG8MG_000103"
     CDS             26275..28467
                     /locus_tag="ACG8MG_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0374183.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28493..29455
                     /gene="mraY"
                     /locus_tag="ACG8MG_000104"
     CDS             28493..29455
                     /gene="mraY"
                     /locus_tag="ACG8MG_000104"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFQ0374184.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29469..30830
                     /gene="murD"
                     /locus_tag="ACG8MG_000105"
     CDS             29469..30830
                     /gene="murD"
                     /locus_tag="ACG8MG_000105"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFQ0374185.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30856..31944
                     /gene="murG"
                     /locus_tag="ACG8MG_000106"
     CDS             30856..31944
                     /gene="murG"
                     /locus_tag="ACG8MG_000106"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0374186.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31959..33179
                     /locus_tag="ACG8MG_000107"
     CDS             31959..33179
                     /locus_tag="ACG8MG_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFQ0374187.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33335..34660
                     /gene="ftsA"
                     /locus_tag="ACG8MG_000108"
     CDS             33335..34660
                     /gene="ftsA"
                     /locus_tag="ACG8MG_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFQ0374188.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34683..35924
                     /gene="ftsZ"
                     /locus_tag="ACG8MG_000109"
     CDS             34683..35924
                     /gene="ftsZ"
                     /locus_tag="ACG8MG_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFQ0374189.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35941..36618
                     /locus_tag="ACG8MG_000110"
     CDS             35941..36618
                     /locus_tag="ACG8MG_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFQ0374190.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36632..37231
                     /locus_tag="ACG8MG_000111"
     CDS             36632..37231
                     /locus_tag="ACG8MG_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFQ0374191.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37312..37533
                     /locus_tag="ACG8MG_000112"
     CDS             37312..37533
                     /locus_tag="ACG8MG_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFQ0374192.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37582..38364
                     /locus_tag="ACG8MG_000113"
     CDS             37582..38364
                     /locus_tag="ACG8MG_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFQ0374193.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38385..39092
                     /locus_tag="ACG8MG_000114"
     CDS             38385..39092
                     /locus_tag="ACG8MG_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFQ0374194.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39354..42152
                     /gene="ileS"
                     /locus_tag="ACG8MG_000115"
     CDS             39354..42152
                     /gene="ileS"
                     /locus_tag="ACG8MG_000115"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFQ0374195.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42201..43727)
                     /gene="zwf"
                     /locus_tag="ACG8MG_000116"
     CDS             complement(42201..43727)
                     /gene="zwf"
                     /locus_tag="ACG8MG_000116"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFQ0374196.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43742..44389)
                     /locus_tag="ACG8MG_000117"
     CDS             complement(43742..44389)
                     /locus_tag="ACG8MG_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0374197.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44573..44902)
                     /locus_tag="ACG8MG_000118"
     CDS             complement(44573..44902)
                     /locus_tag="ACG8MG_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFQ0374198.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45079..45807
                     /gene="sfsA"
                     /locus_tag="ACG8MG_000119"
     CDS             45079..45807
                     /gene="sfsA"
                     /locus_tag="ACG8MG_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFQ0374199.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45823..46836
                     /locus_tag="ACG8MG_000120"
     CDS             45823..46836
                     /locus_tag="ACG8MG_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0374200.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46836..48113
                     /locus_tag="ACG8MG_000121"
     CDS             46836..48113
                     /locus_tag="ACG8MG_000121"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0374201.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48176..50878
                     /locus_tag="ACG8MG_000122"
     CDS             48176..50878
                     /locus_tag="ACG8MG_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0374202.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            51030..51347
                     /locus_tag="ACG8MG_000123"
     CDS             51030..51347
                     /locus_tag="ACG8MG_000123"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0374203.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51377..51697
                     /locus_tag="ACG8MG_000124"
     CDS             51377..51697
                     /locus_tag="ACG8MG_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0374204.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51805..53265
                     /locus_tag="ACG8MG_000125"
     CDS             51805..53265
                     /locus_tag="ACG8MG_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0374205.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53333..53554
                     /locus_tag="ACG8MG_000126"
     CDS             53333..53554
                     /locus_tag="ACG8MG_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374206.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53585..53767
                     /locus_tag="ACG8MG_000127"
     CDS             53585..53767
                     /locus_tag="ACG8MG_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFQ0374207.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53767..54180
                     /locus_tag="ACG8MG_000128"
     CDS             53767..54180
                     /locus_tag="ACG8MG_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFQ0374208.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54303..55484
                     /locus_tag="ACG8MG_000129"
     CDS             54303..55484
                     /locus_tag="ACG8MG_000129"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0374209.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55555..55719)
                     /locus_tag="ACG8MG_000130"
     CDS             complement(55555..55719)
                     /locus_tag="ACG8MG_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374210.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55873..55946
                     /locus_tag="ACG8MG_000131"
     tRNA            55873..55946
                     /locus_tag="ACG8MG_000131"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55907..55909,aa:Arg,seq:cct)
     gene            complement(56015..57154)
                     /locus_tag="ACG8MG_000132"
     CDS             complement(56015..57154)
                     /locus_tag="ACG8MG_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0374211.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAGLLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            57326..57460
                     /locus_tag="ACG8MG_000133"
     CDS             57326..57460
                     /locus_tag="ACG8MG_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374212.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(57453..58088)
                     /locus_tag="ACG8MG_000134"
     CDS             complement(57453..58088)
                     /locus_tag="ACG8MG_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374213.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58201..58836)
                     /locus_tag="ACG8MG_000135"
     CDS             complement(58201..58836)
                     /locus_tag="ACG8MG_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFQ0374214.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58870..59331)
                     /locus_tag="ACG8MG_000136"
     CDS             complement(58870..59331)
                     /locus_tag="ACG8MG_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFQ0374215.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(59461..59892)
                     /locus_tag="ACG8MG_000137"
     CDS             complement(59461..59892)
                     /locus_tag="ACG8MG_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0374216.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(59910..60230)
                     /locus_tag="ACG8MG_000138"
     CDS             complement(59910..60230)
                     /locus_tag="ACG8MG_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0374217.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            60529..61305
                     /locus_tag="ACG8MG_000139"
     CDS             60529..61305
                     /locus_tag="ACG8MG_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFQ0374218.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            61320..61523
                     /locus_tag="ACG8MG_000140"
     CDS             61320..61523
                     /locus_tag="ACG8MG_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0374219.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            61539..61877
                     /locus_tag="ACG8MG_000141"
     CDS             61539..61877
                     /locus_tag="ACG8MG_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFQ0374220.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            61864..62043
                     /locus_tag="ACG8MG_000142"
     CDS             61864..62043
                     /locus_tag="ACG8MG_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374221.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(62086..62556)
                     /locus_tag="ACG8MG_000143"
     CDS             complement(62086..62556)
                     /locus_tag="ACG8MG_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374222.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            62643..63341
                     /locus_tag="ACG8MG_000144"
     CDS             62643..63341
                     /locus_tag="ACG8MG_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFQ0374223.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            63519..63860
                     /locus_tag="ACG8MG_000145"
     CDS             63519..63860
                     /locus_tag="ACG8MG_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374224.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            63853..64524
                     /locus_tag="ACG8MG_000146"
     CDS             63853..64524
                     /locus_tag="ACG8MG_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFQ0374225.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            64530..65222
                     /locus_tag="ACG8MG_000147"
     CDS             64530..65222
                     /locus_tag="ACG8MG_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFQ0374226.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            65219..65968
                     /locus_tag="ACG8MG_000148"
     CDS             65219..65968
                     /locus_tag="ACG8MG_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0374227.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            65980..66249
                     /locus_tag="ACG8MG_000149"
     CDS             65980..66249
                     /locus_tag="ACG8MG_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374228.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            66255..66419
                     /locus_tag="ACG8MG_000150"
     CDS             66255..66419
                     /locus_tag="ACG8MG_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374229.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            66412..66711
                     /locus_tag="ACG8MG_000151"
     CDS             66412..66711
                     /locus_tag="ACG8MG_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374230.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            66711..67013
                     /locus_tag="ACG8MG_000152"
     CDS             66711..67013
                     /locus_tag="ACG8MG_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0374231.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            67053..67418
                     /locus_tag="ACG8MG_000153"
     CDS             67053..67418
                     /locus_tag="ACG8MG_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374232.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            67908..68048
                     /locus_tag="ACG8MG_000154"
     CDS             67908..68048
                     /locus_tag="ACG8MG_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374233.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            68045..68227
                     /locus_tag="ACG8MG_000155"
     CDS             68045..68227
                     /locus_tag="ACG8MG_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374234.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            68240..68434
                     /locus_tag="ACG8MG_000156"
     CDS             68240..68434
                     /locus_tag="ACG8MG_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374235.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(68467..68679)
                     /locus_tag="ACG8MG_000157"
     CDS             complement(68467..68679)
                     /locus_tag="ACG8MG_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374236.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            68807..69007
                     /locus_tag="ACG8MG_000158"
     CDS             68807..69007
                     /locus_tag="ACG8MG_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374237.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            69004..69276
                     /locus_tag="ACG8MG_000159"
     CDS             69004..69276
                     /locus_tag="ACG8MG_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFQ0374238.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            69277..69462
                     /locus_tag="ACG8MG_000160"
     CDS             69277..69462
                     /locus_tag="ACG8MG_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374239.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            69440..69796
                     /locus_tag="ACG8MG_000161"
     CDS             69440..69796
                     /locus_tag="ACG8MG_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374240.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            69756..70232
                     /locus_tag="ACG8MG_000162"
     CDS             69756..70232
                     /locus_tag="ACG8MG_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0374241.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(70378..>70744)
                     /locus_tag="ACG8MG_000163"
     CDS             complement(70378..>70744)
                     /locus_tag="ACG8MG_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFQ0374242.1"
                     /translation="TQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKIKKMLL
                     GKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQFIAADY
                     DADLADQFINGNNFMPTTN"
BASE COUNT        23649 a        11748 c        14800 g        20547 t
ORIGIN      
        1 agatgaggaa atagaattag aaacatataa ttattaaagg gaaacatttt tacagctata
       61 atttgaaaaa atttatcgtt attatatgat taagaaggat tgaataaaga aagaagttga
      121 aaacgatacc tgattacaaa aaaataactt ttataagttc tataacgttg attatatttt
      181 taattcttat tcttctcgtc gtcttggttc cccgaatttt tggtttgact ccttatatta
      241 ttgaggacaa agaaatgagt ccatattatg cacaaggaaa tctgatatat gtacgaccga
      301 aaaatacaaa tgagatttta gtcggagata ttattacata ttatgaaaat tcaggaaaaa
      361 aaatctcgac gcgtcgcgta atagccgttg aaaacaacca cgaagatttt tatacaaaag
      421 ctgatacaaa gacttatatt gagcctggag tagtaaaaaa gagaaatatt atcggtagtc
      481 ccatctttca gattccatat attggactag tcctttcaaa aacggcattt gcatggattc
      541 aggggttatt ctttacgatt gctttttgcc taacaggaat cacagcatgg aatacctttg
      601 tagagttgaa aaagaagcgg agacgaacac agaaattttt gtaagaatgt taaaaacata
      661 aatgtattcc agagatccaa cgactcttct gaacatgaaa cacgagaaaa cactcatgca
      721 gcttgtagat ttagtcagtt gttcgtataa tgtgtgaaga ggaggagtag tatgcgcaat
      781 gaaatgatgc atcgtcctgt agtcaaagaa gaactattgg attttatgcg tacccagcaa
      841 aaaaaattac cgggtgagct aggcaaactg gaagaagaag ctcatgtagc agaagtacca
      901 gttattcccc atgaaacagt tgtatttttg aaatttttac ttggacagct tcagcctgaa
      961 cgaatcttgg agatcggagc agctatcggt ttttcatcaa gtgtcatggc cacgacaatc
     1021 ggagaaaacg ctcatgttac gacaattgat cgtttcgatg tcatgatcga gaaagctaaa
     1081 aaaaactatg aacgtctagg actgacagat aaagtgactt tgcttgaagg tcaagcagca
     1141 gatatcttgc cggagttgtc tggtccgtat gactttattt ttatggatag tgcaaaatca
     1201 aaatatattg aatttttacc tgaatgctta cgtttgttaa gaaaaggtgg cgttttgatg
     1261 gtagatgaca tcttccaagg aggaacgatc ttgctcccag atgaagagat tccccgtggc
     1321 aaacgagcta tccatcgtaa attaaatgaa tttcttcgtg ttgtgatgga tcatcccgat
     1381 ttgacatcga caatcttgcc attaggtgat ggtgtgatct taatgacaaa ggaatcagaa
     1441 acaatcgagt tgtgatacat gattatggca gcctgtagat aatcgattga atcgagggct
     1501 caattcgaac ttgatgtaaa agtttctata tttgtaagtc aattaaagga tacaggaaat
     1561 ctaaataaac aggagtagca tgaagtcaat gtagctcctg ttttcaattg ataaccttag
     1621 aacagctgaa ggagaatgtc tcgttttaaa agattgattt agtagttggg ccaaaataat
     1681 tcattatttg ctaattaaat aactatttcg tattgacaga agcaatttaa ttatctataa
     1741 ttattgatgt tccgctgatt cttgtcatag tagctcagct ggatagagca tccgccttct
     1801 aagcggacgg tcgggggttc gaatccctcc tgtgacgcta aaaaaccgtt agaaactgag
     1861 gtttctaacg gtttttcttt gttttttgtg ccaatattgt ttgttttcaa aggcaaataa
     1921 aataaaaaaa catccttatc gagtacaatt ataaggagca aaaaaataaa actcatcaga
     1981 aaagaggaag aagatatgac gatactatct agaattttag tgactctagt tgcaattgaa
     2041 tttttttata tcatgtatat cgaaacggtg agaactgatt ctgacacgac aagtagggtt
     2101 tttaaaatga gtaaagaaga gttaagcaga aaatctgttc aaacattgtt taagaaccaa
     2161 ggagtgtaca atggactatt aggagtggga ctactttatg gagcttatct ttcttccgca
     2221 tctaaagaaa tcacaagtat gctattaata tctatttttt ttgttgcatt atatggcagt
     2281 ttgactagcg atcgttggat tttactcaaa caaggtgggc tagctctgtt ggcattgctt
     2341 tctattttct tataaatata gaaaaaataa gaatttatct aacaggaaac tttgtgaaaa
     2401 atattacttt atctcatgaa atatgatatt ctaatgaatg gtattttaga aaatttcatg
     2461 aaaaaggagt tagataggtg ttcaaaaatt atgtttcctc actaaaaaaa gaattttcag
     2521 gttatgatct ttcaagattt cagaaagatc ttttggctgg gatcacagta gctgctgtag
     2581 cattgccttt agccttggct tttggggtat caagcggagc tgatgcggcc gcaggtttga
     2641 ttacggctat cattgccggt atcgttattg gcggtttatc aggcggtttt tatcaaatat
     2701 caggtccgac aggtgcgatg gcagctatct tgatgtctat tgcggcaaca aagggaatgc
     2761 aaggtgtatt gcttgctact tttttagcgg gtctattttt acttgcagca ggacttttcc
     2821 gattaggaac actgacttct tttatcccag cacccgtaat tacggggttt acttcaggta
     2881 ttgctatcat tattgcatta ggtcagatcg ataatttatt tggtgttcac tcggaaggta
     2941 caaacgttat tgaaaaacta agtagttatc aaacgatagg atttccgata aacacagcct
     3001 ctttgatcat gggagggcta gtcattttag ggatgtttat ctatccaaaa aaatgggcga
     3061 aacgaatgcc tagttcgctg ctggctatca ttttggcaac agcagttatg ctattgttcc
     3121 atttaccagt agcaactgtg ggtaagattc cacaaacatt gatcagtagt aatcgtttga
     3181 atatgggaga ttttagcttt tctgccttgc aacaagtggc agttccagct attagcattg
     3241 ctttattagg aatgattgaa agcttgctat gtggtgcttc ggctggccga atggcaaata
     3301 agcccttaga tagcaatcaa gaattaatgg cgcaaggaat tggaaatctg ttgcttcctt
     3361 tctttggcgg gattcctgct actgcggcca tcgcccgtac aagcgtggcc atcaaatctg
     3421 gtgcacaaac tagattagct ggtatgatac atgcagttgt cttactgctc tccatgcttg
     3481 tttttgcgcc agtgatgtca cagattccaa tgccagcact tgcaggtgtt ttgattgtga
     3541 cggcttggcg tatgaatgaa tgggaaacga tcaaggagct atttactaat aaatattggt
     3601 cagcagttct attgtttatc cttacgatga tttgtacagt tatctttgat ttaagtattg
     3661 ctatcgtgat cggggtgatc ggcggctgtg tattttttgt tgtcaaaagc gctgctatca
     3721 cgatttcatt agaaacgatc gattggcaaa gaatggggct gccagaaacg gaaaagctga
     3781 ataactggac agtagtgtat attagtggtc cactgttttt tatgtcttca gaaaaactca
     3841 aaaataaatt ggaagaacta aatcatcacg atggtgtcat tttctccatg cgaggcgtac
     3901 caagtattga tttgacggca cgtgcagttt ttgatgaatt tcaagaaaaa gcagagcaaa
     3961 gaggacaaac gattatctat acttccttgc agccagaagt agaaaaacaa ctttctcact
     4021 tgtgggaaca tacattcacg catcaacatc aaacagttgc tcatgcgttg acagctttac
     4081 atgcgaaata tactgcaatt agccattaaa acaatgaaaa agaacagaca agactgtaaa
     4141 ctgaagattt tatggtcttg tctgttcttt ttaatattgc tgtaaaagtg aagctagtac
     4201 ttgccagtct tgatggataa caggtacaag ggagcgattg tagaaaatag ctgctggatg
     4261 aaaaagtgga agaattcgat agtttttttc tgaccattcg taggcatcct tttgttctgt
     4321 taattgtaag acaggccctg tatagagttg accatgattt ttggtaaccg tatattcttt
     4381 gccaagtaaa cgttgaagac caatgtttcc aagtgtcgca attaattttg gctggacctt
     4441 catcagttcg taatccaata ttggtgcatg cgcaaatact tcggaacgag taggtgtccg
     4501 atttggataa acagtttctg actgttgtgt ccgtttattg atccgatgag tcacgcgata
     4561 cgggcggctc cttacagcac tggtgatata cacctcttct cgtgttaagc cagttgaaga
     4621 aagtgcctgc atcaattctt ttccagcttg accgctaaaa ggaacatgcg tatcaatctc
     4681 tgttttccct ggggcttctc ccacgagcat cagtttagga tgaagaggtc cttgcccagc
     4741 gataaatcct tctacaggac ggccgttcat tcttaaagaa gcttcttgta tcagttcttt
     4801 tgggtattcc atttatgctc ctcctttttc ttttattata agaataaaag aaacacaatg
     4861 cgactatttt atttggtaga actgtgagga tagaaaagca gaagtattgc ttgaattttt
     4921 gttttacttt ttgattttaa atttagttga ctaacggttg ctgttctggt aagctagtac
     4981 tgttgtaatt gtggactcca cagctacaac cgctcagaac aagtaataag cacagcatat
     5041 gctgtcactt cggatggcga gtctaagtat atcagaagga ggtgctttat agcatgtacg
     5101 caattattaa aactggtggt aaacaagtaa aagttgaagt gggacaagca atctatgttg
     5161 aaaaacttga cgttgaagca ggagaaaaag ttgtttttga cgaagttatc ttagtaggcg
     5221 gagaatctac aaaagtaggc gctccaactg ttgcaggtgc aactgttgaa ggtactgttg
     5281 aaaaacacgg caaacaaaag aaagtcgtta ctttcaaata caaacctaaa aaacattcac
     5341 accgtaaaca aggtcatcgt caaccatata caaaagtggt tatcgacgcg atcaacgctt
     5401 aatagcgttt gagaaagaag gcctatatat gatcaaagga acatttaagc gaaatgacgc
     5461 aggtcagatc gtttcattca ctttgaccgg gcatgctgat gctggtcctt atggaagtga
     5521 tatcgtttgt gcgggtgttt ctgcgttagc gatcagcact gtcaacggga ttgcctcatt
     5581 agccggtttt gagccgattg ttgaaatgaa tgaagaagaa ggcggttatc tttataccga
     5641 agtcacttca ggaatgacac aagaacaaaa caatattgct gagattctat tagaaaatct
     5701 tttattagga ttacaatcga tagaagctga aaactcagaa tatatccaaa ttaaaacaat
     5761 taatgataaa taggaggtgc agaccatgtt gttatctatg aatctacaat tatttgccca
     5821 caaaaaaggt ggcggttcta catctaacgg ccgtgactca gaatcaaaac gtctaggtgc
     5881 taaacgtgct gatggacaaa cagttacagg tggatcaatt ctttaccgtc aacgcggtac
     5941 aaaaatctat ccaggtgtaa acgtgggtat cggtggagac gacactttat ttgcaaaagt
     6001 tgatggcgta gtacgtttcg aacgtaaagg ccgtaacaaa aaacaagtgt ctgtttatcc
     6061 agtagctcaa gaagcttaat tgaatcttat gattaaagag gttgtgacaa gacttttgtc
     6121 acagcctttt ttgtttataa aaataaaagt tgtagtggag gctacgaagt ttactttgtc
     6181 tttttacact taatattttt ttgccaattt ggaatcagga tgttttgata actatcgttt
     6241 tcaattgatg gaagctatat tgtttgctat cagtgaaata attatatggt aaacagtgtt
     6301 ctttttcttg tttccattat aaaaatcgta tcgggatttt tcgttctttt aaattgaaca
     6361 tttctatcta tccgcgtgtt atcaacaaaa caaaatcagg taaatagttg acgaaacaga
     6421 aataaataca tagtattatg ttttgaagcc taagtacaat agtctataga aaaggagcga
     6481 taccattggc aaaacgcatt gaccgaattt atatgtatgt caaagaaaaa accgctcatt
     6541 taacaaaggc agaatatgat caaggagtaa caacacagga aattgctgaa gtgttaggga
     6601 ttcagcgaac aaatagcagt aaagatctga atcagctagt gcgcgaagga aaactgataa
     6661 agacagacgg acgaccagta cgttacatat atcaaacact cgttacgcac gtaaagccag
     6721 ttacaaaaca tgtagtcagt tacaaagaaa cgccggtctc tgaagaaaaa aatacactcc
     6781 aaatagatac taaggatatc tttgcaaaaa tcattggagc taacggcagc atgaaaaatt
     6841 cagtggaaca ggcaaaagct gcgattttgt atccgccaag aggattaaat tgtttgatta
     6901 caggacctac cggatcagga aagacttatt ttgcacacgc gatgtatcat tttgcaaaag
     6961 cgcatcatgt aatagatgag gaaacggaac tgattgtttt caattgtgct gattatgcca
     7021 ataaccctga gcttttgatg agtcatttat ttgggtacgc aaaaggtgct tttacaggcg
     7081 cagatgaaga aaaaatgggg attatcgatc aagcggatgg cggtatgctg tttttggatg
     7141 agattcaccg gttgccgcct gaaggtcaag aaatgatctt ttactttatg gatcatggaa
     7201 cttacagtcg tcttggggaa acaacgaaat cccatgaagc aaatgtacgg atcgtaggtg
     7261 cgaccacaga agacccaggt tcttctctat tagaaacttt tgttcggcgt atcccaatca
     7321 atatcaaact tccggctttt gaaaaacgtc ctgcaaatga gaaagtcgat ttagtaaaaa
     7381 tcatgattgc ccacgaagca aaccgcacgc agcgaaagat ttctttgaca gaagatgtag
     7441 caaaagcatt gatcggcagt gtgacctatg gaaatatagg acaattgaag tcaaatatcc
     7501 agttagtatg tgcacgagga tttctaaatc atatgcaatc acccgaaatc tcgatcacaa
     7561 tcgatgattt gacagaaggg attcgtagcg gattgattca attagcaagc aaccgcagtg
     7621 caatggcaga gctttctaaa atattggagc caaagataac tgtagcacca aatgatacga
     7681 tcatgaaaat ccaatcggac tcctatgagt taccttataa tctatatgat atcatcggag
     7741 ataaagcagc tttactgaag tcggatggtt tggatcaaga agcaatcaat catttcattt
     7801 ctacagatat caacatccat ctgaagtctt tctataaaga ccatggtttt tcttttaatg
     7861 cagacagcaa attagcagaa tttgtagatc cgaaaatcat agaagtgacc aatcagattt
     7921 atgtaatggt caaaagatct ttaccatatg agtttcagca aaactttatt tatgctatga
     7981 gcttgcatat cagttctttt ttgaaaaaaa tcaacttagg agaagaacgg catacgaatg
     8041 ataacatccg tgagatggct tttgattttc cagatgaata tgctgttgcg aaagaagtgc
     8101 gtagttatat cgaaacgtat tttcaagtgg agattccaga aagtgaagac tattacttga
     8161 ccgttctact cgtctcgtta cgggcaaatc aagcaagcgg cagaatcggt gtagtcgttg
     8221 cagctcacgg aaacagcaca gcaagcagta tggtccaagt tgtccaacag ttgttagatg
     8281 cagagaacgt acgagcagta gacatgcctt tggatatgga tccgaaaaca gcattggacc
     8341 gaattgaacg caatgtacaa gaagtagatg aaggaagcgg ggtcatactc ttagtagata
     8401 tggggtccct tgcgtcgttt aattcgcaaa ttcaaagaga cactggaatt ccagtacgta
     8461 ctgttgatat ggtaacaact tctctagttt tggaaactgt acgaaaggtc tcagtattag
     8521 ggacagatct agatatgttg catgaatcat tgaaaaattt tcgaggttac gcagaaatat
     8581 cttctgtaga atctgttcag gaagcaaaac ctattcataa gaaaaaagct gttttggcta
     8641 tctgcgcatc tggcgaaggt acagcacaaa gaatcaaaga attgatcgaa cgagcggtaa
     8701 acaagcgtca agaaacagaa ttagaagttc tagctttgtc gattgtggaa gtgaaagaag
     8761 aactgccagt tatccaaaaa gaatatcaga tcatagctac tactgggatt actgatttga
     8821 atatccaagc cccatttatt cctttagaac gttttattga tcaaaatatc gaagtcattt
     8881 tggatcagtt gctgatggaa tcagaattgg aggaaactga attcatctcc ctagatgaag
     8941 agtcagctaa aaatacgtgt gttgagttta tctctgataa cttcatcttt attaatggta
     9001 gtaagttaat cgatccaatg tggcaatttt ctacacaaat ctctcagacg acgggaatcg
     9061 gcgatgaaga atatggattc aaaatcaacc tagtgatgca cacagcagga atgatcgaac
     9121 gtattattcg gaatgaacca ttgactgtgg aagaaaacga attgacaaat acaacgaatg
     9181 atcctcttta tagccaattg gcagcctcag ttgttctgtt ggaagatcag ataaaagtaa
     9241 aagtgccgat agaagaaatg tattatctgc tacgtctagt ccataatcag ttagataaaa
     9301 aagaatacac tgtgccatga atacacaaaa acgatacact tttatagtgt atcgtttttg
     9361 tgtattggtt aagaatcctt taaattcaat tagtgtatcg taaaaaagtt ggcacgcttt
     9421 ttgcttatag tagtgtgtgc taggaggtgg ttgttcacac agaaatgata ggggtttctg
     9481 tgaagtagtg aggaagaagg agttttgcag atggatattc gattggcacg tatagatgat
     9541 cgtttaatcc acgggcaagt cgcaacagtt tggtcgaagc aaacaggtgt tgaacgtatc
     9601 attgtgatca gcgatgtagt cgctcaagat aaattgagaa agtttctact aaaagaagct
     9661 gcaccgccgg ggatcacagc aaatgtaata accgtgcaga aaatgattca aatctatcat
     9721 agtggattat tatcacgagc aaaagtgatg cttttattca ccaatcccca agatgttgaa
     9781 gcggttgtcc gctgcggtgt acagttgaag tcgttgaata tcggcggtat gagttttact
     9841 gaaggcaaat caatgattac caattttgtt tcagtaaatc aacaagatat tgattccttt
     9901 cgttacttga acagtcaaga agtggagtta gagattcgaa aagtacctgc tgacaggaaa
     9961 atccttttga tggaactact agcgaaaatc aaaaaggtgt agcaagaatg gttgttggaa
    10021 aaggtcaaat aattctgttg taatggttag caattattaa tatttttgaa tttgttacaa
    10081 caaaagtaaa taggaggtat aaatatggta gggattatcc tagcaagtca tggacaattt
    10141 gctgaaggaa tcttacaatc cggttcaatg atctttggcg aacaagaaaa cgtcaaagcc
    10201 gtgattttga aaccaagtga aggtccagat gacttgagag ccaaattgga agaagcggtt
    10261 gcttcttttg ataatcaaga cgaagtgtta ttcttagttg atttatgggg cggaacaccg
    10321 ttcaatcaat caaacacatt attcgaagag cacaaagata aatgggcaat cgtcagcggt
    10381 ttgaatttac caatgttgat tgaagcctac gcatcacgct tttcaatgga atctgctcat
    10441 gaaattgcag cacacatcat tgaaacagca aaagacggtg taaaagtaaa accggaagaa
    10501 ttagaaccgg cagaagcacc gaaagcagca gttgaagacg cacaacctaa aggagcactt
    10561 ccagaaggaa ctgtggtcgg agacggaaaa atcaaatatg tattagcacg tgttgactca
    10621 cgtctgttac atggacaagt tgcgacagct tggacaaaag cagtgcaacc aaaccgtatt
    10681 atcgttgttt cagatgcagt atcaaaggat gatctgcgta agagactaat cgaacaagct
    10741 gctcctccag gtgtaaaagc aaatgttatt cctattagca aaatgatcga agtagcaaaa
    10801 gaccctcgtt ttggaaatac caaagcattg cttttatttg aaaatcctga agacgtattg
    10861 acagctgttg aaggtggcgt agacatcaaa gaattaaatg ttgggtcaat ggcgcattcg
    10921 gtcggtaaag tcgttgtaag taaagtattg tctatgggac aagaagacgt tgaagcgttt
    10981 gagaagcttg aacaaaaagg tgtgaaattc gatgtacgta aagtacctaa tgattcacgc
    11041 gataacatgg atgatatttt gaaaaaagca aaagctgaat tagcaaaagc gtaataaata
    11101 atattaaaat ttataggagg cttatcatgt ctattatttc aataatttta gtcgtattag
    11161 ttgcctttct agctggtatg gaaggtattc ttgatgaatt tcaatttcat caaccactag
    11221 tggcatgtac attgatcggt ttagttaccg gtaacctcga agctggtatc gtacttggcg
    11281 gtacgcttca gatgatcgct ttaggttggg caaatatcgg tgccgctgta gcaccagatg
    11341 ctgcattagc atcagtagca tcagcaatta ttttagtttt aggtggacaa ggtgttaaag
    11401 gtgttccttc tgcgatcgcg attgccgttc ctttggctgt tgcagggttg ttcttaacaa
    11461 tgatcgtacg tacaatcgct gttccaatcg ttcatttgat ggatgcggct gctgaagaag
    11521 gaaacatccg taaggttgaa atgtggcaca tcattgctgt atgtttacaa ggggttcgta
    11581 tcgcggtccc agcagcagcg ttgctattta tcccagcaca aactgtgcaa tcattccttg
    11641 aatcaatgcc agcatggtta acagatggta tggctatcgg tgggggaatg gtcgtagctg
    11701 taggttacgc tttggtaatc aacatgatgg ctacaaaaga agtatggcca ttcttcgtta
    11761 ttggtttcgt tgtagctgca atttctcaat taacattgat tgctctaggt gctttaggtg
    11821 ttgcattagc tcttatttac ttgaaccttt ctaaaatggg tggttcatca aataatggag
    11881 gcggaagcaa ctctggtgac cctttaggcg acattttgaa tgactattaa tcttgaagga
    11941 ggaaaataaa atggcagaag aaaaaatcaa attatcaaaa aaagatcgtt tagctgttgc
    12001 gtggcgctct accttcattc aaggttcatg gaactacgaa cgtatgcaaa acggtggctg
    12061 ggcattttct atgattccag caatcaaaaa attatacaaa acaaaagaag aacgttctgc
    12121 agcattaaaa cgtcacttgg aattctttaa tacacaccca tacattgctt ctccaatttt
    12181 aggggttaca ttggctctgg aagaagaacg tgcgaatggc gcgcctgttg atgatgtagc
    12241 gattcaaggg gttaaagtag gtatgatggg tcctttagct ggtgttggtg acccagtgtt
    12301 ctggtttaca gtacgtccaa tgctaggtgc actaggtgct tcattagcta tgggaggaaa
    12361 tatccttgga ccaatcatct tcttcttagg ttggaacatt attcgttggg cattcatgtg
    12421 gtatacacaa gaatttggct acaaagctgg ttctaaaatt actgatgacc tttctggcgg
    12481 attgttacaa gacgtaacaa aaggtgcttc aattttaggg atgttcgtat tagctgccct
    12541 tgtacaaaga tgggtatcaa tcaaattctt accagttgta tcaactgtaa aattagataa
    12601 aggtgcttac atcgaatggg ataaattgcc ttcaggtggt gaaggaatca agagtgcatt
    12661 tgaacaagtt aataatggct tagccctttc tcctgaaaag gtaacaacat tacaagacaa
    12721 cttagaccaa ttgattcctg gtttagctgc attaggatta acattgttat gtatgtggtt
    12781 gcttaagaaa aaggtttctc caatcgttat catccttggt ttattcgtag tcggtgttgt
    12841 tggacacgtc atcggacttt tataatccat aaaaatatag ctcgggcgtt gtcccgagct
    12901 ttttttatat agtatactgt tcataaacaa atatctggag ggagaaaatg gttcaatcaa
    12961 tcaatacaaa agtagacttg gtaattgatg ctacttcgtt tacgggtctg acagattacg
    13021 ggcaaatcat gataggtaat aatggttttg agttttacca tgcgcgagat agacgcaaat
    13081 atatccaaat tccatgggaa gaagtggatt atgtactagc atcagtcatg ctaaagggaa
    13141 aatggattcc tcgatatgcc attcaaacga aaaaaaatgg tacatacaca ttttcttcca
    13201 aagaacctaa aaaagtgctt cgtgcgatca gagaatatgt agatgaagat agaatggtac
    13261 aatcattgag tttctttgat gtagtgaaac gtggtattcg tagtatgttt aaacgcaagt
    13321 aaaaaataaa gaaaacttct aagaggaggt tttctttatt tttttacttt gcagaagaaa
    13381 atatgaaata gcaaaaccaa ttatttgtcc cctttaaatc ccatatgcta aaattgatta
    13441 gcaaagaagg aggttatatt atgagttggt tatggacatt gattgttgga gccgtgatcg
    13501 gagcaattgc aggtgcgatc accagtaaag gaaattcgat gggctgtgta acgaatatca
    13561 ttgctggact agtcggttca agtattggac aagcgatttt cggcgaatgg ggaccaaaag
    13621 ctgcaggtat ggcacttatt ccatctattt taggcgcagt gattttggta gcagttgtct
    13681 catttttctt tggaaaacga tcataagttt ttacaaaata gtaaactaaa aggagttgca
    13741 gcgagactgt gactcctttt tggctttatt tatttaaaat aaaaagaagt tttccggaaa
    13801 tttttattta actgtcgtgt acgaagaaaa gtttgctata atggaaaaaa gcgtgcaaag
    13861 aaagagggag tttcattatg atgctacgtg tagaaaaatt gcgcaaaaaa atgcaagaag
    13921 aaaatttgga ttcatttttg gttaccagtc cgtacaatct tcgttactta acgaacttta
    13981 caggtacgac aggattagct gttattactt tggaaaaagc attttttatt actgattttc
    14041 gttataccga gcaggcggca gcgcaggctc aagggtttga aattattaaa aatgtcggac
    14101 ctatttttga tgaggtagca aacttagtac gcaaagaaga actgcactca ttagcttttg
    14161 aggaaacaac agtctcattt ttagaatatt cggttttaga agaaatcatc gatgcggaat
    14221 tagtacctgt ttcacagatg atcgaagaac tcagagaagt gaaagatgaa gaagaaatcg
    14281 ctattatcga aaaagcatgt catattgcgg atatggcata tgatcacatc ctgaaaatga
    14341 tccaaccggg catgactgag attgaagtcg caaatcaact agacttttat atgcgttctc
    14401 ttggcgcttc aggtgtttct tttgatacga tcgttgctag cggattgcgt tcggctatgc
    14461 cgcatggtgt agcaagcaaa aaaatcatcg aacaaggtga cttgatcaca ttggactttg
    14521 gttgctatta cgaagggtac gtttccgata tgacacgtac attcgctatt ggtgatcctg
    14581 gggaaaaatt aaaagaaatt tatcaaatag tcttagatgc tcaattagca gttattgatg
    14641 ctgcaaaacc aggaatgact ggtgtccaat tagatgcagt tgctagagat tatatttcaa
    14701 aacatggtta cggggaagca tttggacatt ctacaggaca tgggatcggt ttagagatcc
    14761 acgaaggacc aaacgtttct tctcgtgcag aaaagcaatt tgtaccagga aatgtgatca
    14821 ctgatgaacc agggatctac ttaccaggaa tcggtggagt acgtattgag gatgacttgt
    14881 tgatcacaga agatggaaat cgtgtgttga ctcgttcacc aaaagaattg atcatcttat
    14941 agtttcatca atagaagaag taagcgtaaa aatcagtgtt cacagacttt gttatagaaa
    15001 atcactcgat ttaatgatac actatagaag aacttttaag ctgtaagaga taataaagtt
    15061 gggtgttttc tccaattcat agcagtttgc agctgatcgg cggcagtttt cttaccacgt
    15121 gatcactatg ccgcatcata gagaattcgg atgtatctcg tgcttaggca aagggaggaa
    15181 taaataaatg gcagatgaaa aaaatttgat tttaaacgca aaccaagatt taggagagat
    15241 cgtgatcgct cctgaagtca tcgaagtaat tatcgggatt gcagcttcta aagtagaagg
    15301 cgtatacgga atgcgcggaa cttttgccag caatgtcact gaattattag gacgtgcagc
    15361 tcacggaaaa ggtgtttact tagcaaacga ggaagatggc ttgaaagtag atctttactg
    15421 ttatctagaa tatggtgttt cagttcctaa agtagcaatg gaaatgcagg aacgggtcag
    15481 ccagcaagtc cttttcatga cggatatcga tttatctgaa gtcaatatcc atgtagtagc
    15541 tgttgttcct gaaaaattag aacaaccaac aatcgaagaa ctttttgaag acgaagagga
    15601 agagaatgaa taaagaatta tcaagacacg agattcggga aatggcattg caggctttat
    15661 ttccattaga cttcaatgca gacctaacaa aagaagatgc gattttcaac gcaatcgaat
    15721 tagaccaccg cgacatgatc aacgaagatg aatcagagtt tgttcctgtt tatcttgata
    15781 cgctagttgg cggtgtctgt gcaaaaaaag atgaattgga taaagtgatt gaaaaacatt
    15841 taaaaaacaa ttggcacatc aatcggatct caaaaatgga tttggtgatc ttgcgtatcg
    15901 cgatttttga aatgacttat gtgacagatg taccagcggc tgttgcttta aatgaagcaa
    15961 tcgagttggc aaaaacattt agtgatgacc gttcacgtaa attcgtcaac ggtgttttat
    16021 ccaatgtatt gaaagaatta gaagctgggg cgtaagtcct agcttttttc tatgaatcat
    16081 cataaacctg ttataaatcg ggaaaattct atggtagaat gaaaacacaa aaaatatctg
    16141 ggagtggtta gatggcagaa ttgattaacg gaaaagaatt agctgaaaaa atgcaggcag
    16201 agattgctga aaaaatccaa aaattgaaag ataatggtat ccatcccgga ttagtcgttt
    16261 tattggtagg ggaaaatcca gccagccaaa tctatgtaag aaataaagaa cgtgtagcaa
    16321 aagaaatcgg aatccattca ttggtagagc gttatcctga gacgatttct gaagacgaac
    16381 tacttgcaga aattgaaaaa tacaatcaag atcctagttt tcatggtata ttggtccaat
    16441 taccattgcc aaagcatata gatgaagaaa agatcctgtt agctatccgc cctgaaaaag
    16501 acgtagacgg ttttcatccg atgaatttag gcagactcct tgcgggtaaa ccggacaaga
    16561 ttccttgtac tccatacggc atcatgaaaa tgtttgaagc ttattctatc gatccggcgg
    16621 gtaaacgagc agtagttatc ggacgaagca atatcgtcgg taaaccgatg gctcagctac
    16681 tcttgatggc agatgcaact gttactgttg ctcattctaa aaccgaaaat ctggctgaac
    16741 tggcacaaga agcagatatt ttagtcgtag caataggacg tggacatttt gtcacaaaag
    16801 aattcgtaaa accaggtgct gtagtgatag atgtagggat gaatcgtgat gctaacggta
    16861 agctgatcgg tgatgtgaaa tttgatgaag tggagcctat tgcaggctgg atcactcctg
    16921 tgccaaaggg tgtagggcca atgactatca cgatgctgat gtatcaaacc gtaaaatctg
    16981 ccgaagcttt tggagcaggt gaatagatgg cacaagaata tttaactgtc actacattaa
    17041 cgaagtattt gaaaagaaaa tttgatgccg atccttattt agaacgcgta tatttaacag
    17101 gcgaaatatc gaatttccgt ttacgtgcca atgcccatca atatttcagc ttaaaagatg
    17161 accatgcaaa aatatctgct attatgttca aaggagcatt ccaaaaatta cgattccagc
    17221 caaaagaagg aatgaaagtc cttgtcgttg ggcgtatttc attatatgaa gcgggaggtt
    17281 cttatcaaat ctatgtagag cacatggagc ctgatggaat aggtgcttta tatcaagaat
    17341 tggaagaacg aaaagaaaag ctgatgaagg aaggcctttt tcaagggcct aaaaaaatgc
    17401 tgccacgttt cccaaagcga atcgcggtcc ttaccagtcc aagcggagcg gttatccgcg
    17461 atatcatcac gacggtgaaa agaagatatc caattgtcca attggttttg ttcccaacag
    17521 ttgtacaagg aaaccaagca gcggacaatg ttgtcaggaa tatccagaga gtagaagaga
    17581 tgggcaattt cgatacaatg atcatcggtc gtgggggtgg ttcgattgag gatttatggc
    17641 cgtttaatga agagcgggtt gctcgggcga tttatcaagc taatacgccg gtgatttcat
    17701 ctgtaggaca tgaaacagat accacgatcg ctgatttagt agcagatgtt cgggctgcca
    17761 ctccaacagc agctgcagaa ttagctgttc ctgttttgtc agaagagttg atgcgcattc
    17821 aagaacggca agctagattg cagcaggcgt ttgtacgcca gatccagcgc aaacaagaac
    17881 ggttcgagcg tgctcgacgt tcctatgtat ttcggcagcc tgaaagattg tatgaaggtc
    17941 agaccatcaa attagaccag ttgaaacagc gattatatca agctacacag caaatttatc
    18001 atgcgaaaga aaaacaaacg atcgcattaa gccaccaatt ggaacaagtg gctccgcttt
    18061 atcgagttaa agcagctcgc caagaaactg cttatctaga aaaacgctta gaagagaaaa
    18121 tccaacaata tatgaaacaa cagcagcagc gttttgctca agctgttcaa tctcttgatt
    18181 tgttgagtcc attgaaaatc atgggaagag gctacagcta tacgacgtta aatgatcaag
    18241 tagtaaaaag tgtcaccgag attcatcaag gggatttgct gacgatccat tatcaagatg
    18301 gaatcgttaa aggacaagta caagagatag acaaaggaga ataaaaatat ggaaaatcca
    18361 acttttgaag agtcactgca agaattagaa aaaattgtca tgcagttaga acaaggagac
    18421 gttccattag aatcagctct agattcgttt aaacgaggaa tggaattaag caaacattgc
    18481 caagatacat tagctaaagc agaagagacc ttgacgaaaa tgatgacaga atcaaatgaa
    18541 gaagttgatt ttgacggaaa cgaggaaagc tgagatgaga ttaactgact tttcagctat
    18601 tcatctacct gctgtagaaa aagaaatctt gtctttttta gatgagcata caacggatag
    18661 aggactctat gatgcgatga gttattcagt gaaagcaggc gggaaaagaa tacgtccctt
    18721 attactttta acggccgttg cttcctttga tgagcctatt gatgttcccg tctatcaagt
    18781 agcagctgct ttagaaatgg tgcatacgta ttccttgatc cacgatgatt tgcctgcgat
    18841 ggacaatgat gatcttcgta gaggaaaacc taccaatcat aaagtattcg gcgaagcatt
    18901 agctatattg gccggagatg gtttgcttac aggagcattc cagctaatca gtatggcaca
    18961 cttaggtaat tctcctaagc ttctgctgct tcagcaatta gctgtttgtg cgggatcaca
    19021 aggtatggtt gctgggcaag ctgcagatat cgagggcgaa tcgaaaaagc tttctttaga
    19081 agaattagca tttattcatg aaagaaagac agggcatttg attcgttatg ccttgctggc
    19141 tggtggcata ttagcgaagc aacctgaaga aatcttattg ctacttcaac gattagccga
    19201 acatttaggt ttagcttttc agataagaga tgatctactc gatgtgattg gtacaacgaa
    19261 aacattaggg aaaacagctg gtaaagatga acgtatggaa aaaaatactt acccgcgttt
    19321 attaggccta gaaaagacac gtgaggcgtt ggagattgag ctgttatccg ccaacaagat
    19381 catagacaaa ttagaagagg acgttttttc ttttgatggc gggctgatgc gccagatgat
    19441 caagcaattt gatgtggaga aatgagggaa agaatggaaa aagaacgcgt agatgtatta
    19501 gcggtcaaac aaggattatt tgaaacaaga gaacaagcca aacgttcagt gatggctggt
    19561 ttgatttaca acgaaaaaaa cgagcggttt gataaaccgg gagaaaaaat tccagtgagt
    19621 agtgaattaa gagtcaaagg taaaaaactt ccttatgttt ctcgaggggg attaaaatta
    19681 gaaaaagcac tcaaagcatt tgatctatct gttgaaggaa aaacattgtt agatattggt
    19741 gcatccactg gcggatttac tgatgcagct ttgcaaaatg gcgcaaaaat gagttatgct
    19801 ttagacgttg gctacaatca gctagcttgg aaaataagac aagatcctag agtcgtcgta
    19861 atggaaagag tgaattttcg ttatgcaaaa cccgaagact ttaccttagg aattccagaa
    19921 gttgctgtta tcgatgtttc ttttatttca ctaaaactga tgctgcctcc attacatgct
    19981 attttaaaag aagagggaga agtgattgct ttaatcaaac cccaattcga agcaggcaga
    20041 gaagctgtag ggaaaaacgg aattgttcgt gaccctcaga cccatcaaaa agttttagaa
    20101 gacattttgt catttgcggc acaaggtgga tatgatgtgc tggaattaag ttattctccg
    20161 atcacggggg gagaaggaaa tatcgagttc ttggctcact tgaggaaagt acccgaaagc
    20221 ggaacaatca attcagctat taatatggca gaagtggttt caaatgcaca cgaacagttt
    20281 gaccataaat aaattgtaac tgtgaaaatc aagtgagaag tatgcggatt cttctttatt
    20341 ataccaagga aataagctat cattgattaa gaagaatatg ctaaggagcg aagacgtaat
    20401 gagaaaaaaa gatcggcatc gtttgattac tcgattgttg agtgagcaag atatccgaaa
    20461 gcaggaagaa tttgttgaaa tcctgaaaag caaaggaata tctgttactc aagctacgat
    20521 ttccagagat ataaaggaac tgaaactgat caaggtaccg gcttcagatg gcggctaccg
    20581 ttatagcata ccagctgaaa caaacgaaga tgtaaatact aaattggaaa aactattaaa
    20641 agacgctttc gtggcagttg accaaatgga aaaatttgtt attttgaaaa cattgccggg
    20701 aaatgcttcg gcagcagcaa atttgattga taaacgttac aagaaagaat tattttcgat
    20761 tatcaatgat gacgataatg ttttgatgat tacacgaact gaagaccagg cagttttatt
    20821 gaagaaagac ttcctgcatt acctttaagt tgaggagcga atgtgatgct acaagaaatc
    20881 agtattacta actttgcgat tattccggaa ttgcggcttt cttttcacga aggaatgacc
    20941 gcgctgaccg gagagactgg tgcgggtaaa tccattatta tcgatgcttt aggtctgtta
    21001 gcaggaggaa gaggttcaag cgattatata cgtcaaggag cagaaaaatg tgtgcttgaa
    21061 gggttgtttg aactaccaaa acaagaagga ttctctgaac tgatggttga attagggatc
    21121 gaaacagatg aggacaatct gattgttcga cgggatatgt ccttaactgg aaaaaatgtt
    21181 tgtcgagtga atggtcatat tatcacatta gctaatctga gaagaatcgg cagttattta
    21241 gtagatatcc aaggacaaaa cgaacatcag gaactgctcc agcctgaatc acatcttgct
    21301 ttattggatc gttttggaga tgtagctttc caacagaaga aaaagagtta tcagcaagaa
    21361 tatatctctt atcgtgagct ggaaaaaagg gtacgaaaag tgcagcaaaa tgaaaaatcc
    21421 tatgttcaga gaatagatat gctccatttc cagcaagaag aaatagctgc tgctgatttg
    21481 caagtcggag aagaagagaa attaaaagaa gaaagagaaa aacttagcaa ttatcaaaaa
    21541 attgctgatg gattagctgc aggttacgga gcactgacga ataatgaaca aaacagtgta
    21601 gatggtgttg gcttggccgt ttcagaactg caagggatcg cccatctaga tgtggagtat
    21661 gaagcaattt atgaaaatat ccaaagtgct tactatttgc tgcaagatgc aatcggcgat
    21721 atgagtcgtc agattgattt attggagtta gatgagagtc gtttagaaga agttacgcaa
    21781 cgtttagaat taatccgaca gttgaaacgt aaatacggcg aatccattga gtcaatattg
    21841 gcttactatg acgaaatcac cgaagagcta gcttcttctg atttttcaga aggtcaattg
    21901 gacaaaatga aagaggaact agagcaaaaa gagcttctac ttcagcaaca agccgcagat
    21961 cttcatgaag cgcgtaaaaa aatcgcaaaa gaattggaaa aatccattct gcatgaattg
    22021 aaaagtctgt atatggaaaa cactgagttt gaggtacgtt tcttaaaaga agaaaataga
    22081 caattgaatc atgatggatt tgaccaaatc gaattttaca ttacgaccaa tccaggagag
    22141 ccactgaagc ctttggtgaa agtcgcttct ggtggcgaac tgtctcggat gttgcttgct
    22201 ttgaaaacta ttttttcttc tgagcaaggt gtgacaagta ttatttttga tgaagtggat
    22261 acaggtgtga gcggaagagt tgcacaggcc atcgcagata aaatttctca aatatcgaaa
    22321 tactctcaag ttttgtgcat cactcatctg cctcaagttg ctgcagtcgc cgactatcaa
    22381 tattatattg tgaaagcagt gattgatgga cgtacgcaaa cttctgtttc agagttgaaa
    22441 acaaaagaaa gagaagaaga aattgcacgt atgctggctg gaagtgagat tacaaaactc
    22501 acactcgaac atgcaaaaga actattgaaa ctcgcaaaaa ggacctgagc cacctcgcag
    22561 tggttcaggt ccttttcagc agctgatagc tatattttat ttttacatga cactcattac
    22621 agcagtcaat acaacagatc caatcgttaa aatcagcatt gtccaaatga caaattttgt
    22681 tactttagca aaagtacttg tttgtttttt ttcacgcatt gttttcaacc tcgtcattaa
    22741 ttagttgatt caagtgtaca atcttttttc catttccaca agagggcttt tttataaaaa
    22801 cttccctttt tttaaataat aggtagttac gatacaaata aataatgtta atagaactag
    22861 ccatataaag gcatcatccc tttcagcaat aggtaactta atgttcattc catagatact
    22921 gccgacgatt gtcggtatag ttaaaataat cgttaaagaa gttaaaattt tcataatatt
    22981 attcagatta ttcgatacga ttgctgaaaa agtctgattc atttgttcta ctagttttaa
    23041 ttgaatgcga gaagtagtca ttgcctgctc tgtttccacc aatacatcac gtaaatgatt
    23101 tttcaaagca tgattttctt ggaaaagttt attttgcgct agttctttca atgcatctaa
    23161 attagcagaa gtcgcttctc tgaaataaac gaggcttttt tggatatcca tgatttgata
    23221 aagttgtttg ttttcagtgg atacttgaag tttggcttcc aaagtatcgc attgagattg
    23281 aagcgtagct aaataactgt tgtaagagag tgtgacctgc caaatgaatt gaaagaagat
    23341 attgagatta ggcgaaatat cactttttgg aaaggtctgg ctaaagactt tctttaaaaa
    23401 cagtggttca tgattcgtaa ttgtgataag cttctttttg ggcgtcaaaa taatagagag
    23461 cgggtatgta tccacttgaa aataaccgct aggacttgtt ttagcaaaag gatattgtaa
    23521 caataataac gctggctttg tcaactttga ttgattgaat tcttctgtac gggaattttc
    23581 tgcatcgtcc aatatggaag tgatgtaatc tgacggaatt tcaaattgat gtgtcagctg
    23641 atgaatctct tcttgattag gacgttctac acaataccag atcgtttggt cagtttcctt
    23701 tccggtatta cgcagtttct cattatctaa tgtaaaatat ttgatcatga ttttcctcct
    23761 gttgcctgtt tcttttatta taacggatag ggaattggaa tacatggaat gtgtttgtaa
    23821 gatatagaat ataactgcat aaatttaaat aaaatgaaac agttgtgtaa agtatataca
    23881 gtatacttga agcaacgata aaaaaggagt ttacgttcat gggcacattc acaccactta
    23941 tcttgttttg gtatatttac ccggtaatcg ttttgtttgg ttgccaattt cttgtaaaat
    24001 tattttcgtt aaatcgtcgt tttaaaataa aagcccctga tttggcgatt ccttttttgt
    24061 ggggcggtat gcatgcattg tctagaaata cagggacaat ttctatctta ccgtttttct
    24121 ttatttctgt tttgctaatg ggaatttgta ttgctgtttt ccaagcatat tattatgaag
    24181 aaattattta tccgcgttat ttcaagatga cttggcgttt ggtttttctg ttgacaatcg
    24241 tcttgtacgc tgtcttgatc attgtgaaca tactttctta cctatgaaaa aaatagaaaa
    24301 gctgtaaaaa gtcttttaaa aggctttttg cagcttgatt tttaaaaaaa tgtttatgaa
    24361 taaaaaaagt tgttcccgga tttcccagaa aaggtggtag aaagtggggg attgtggtag
    24421 actaatttta gttagtggag gaatgggggg ctttcaatgt tgatgggtga attccaacat
    24481 aatattgatg caaaaggacg tttaatcgtg ccctcaaaac ttcgtgaaga acttggcgaa
    24541 aagtttgttc tgacacgcgg attggatggc tgtcttttcg gctatccgat gagcgaatgg
    24601 gaaaatctgg aagctaaact gaacgaaatg ccgttggcta aaaaagacgc acgtactttt
    24661 gtgcgattct tctattcggc tgccactgaa tgcgaacttg ataaacaagg acgaatcaat
    24721 atacccagca cgttaagaaa ttatgcagct ttgacaaaag gttgcgtagt catcggtgta
    24781 tctaaccgaa ttgaaatttg ggatgagaca cgatggcaag atttttctgc agcagcagaa
    24841 gagaactttg acgaaattgc tgaaagtatg attgatttcg gactataaga ataggaagag
    24901 gctgactaat gactgaaaca tttcaacact atacagtcat gttgaaagaa acggtagatg
    24961 gattgaacat caaaccggat ggcgtttatg tagactgtac actcggcggt gccggacata
    25021 gtgaatattt attaagccaa ctaagttcta aagggcatct gtatgcgttt gaccaagatc
    25081 aaaaagcaat cgatcacgcc aagctgcgtt tagcttctta tgtcgaacaa ggtatggtca
    25141 catttatcaa agcgaacttc agagaattag aagaacgctt gagtgagcat gtagataagg
    25201 tagacggcat tttgtatgat ctaggggttt cctctcctca gctagatgaa gcagaacgtg
    25261 gatttagtta tcatcaagat gcaccgttgg atatgcgcat ggatcagtca gctcctttat
    25321 ccgcttatca cgtaattaat gaatattcct accatgaatt agtaaagatt ttcttccgct
    25381 atggcgagga gaaattttcc aaacaaattg ctcgtgaaat cgaacgagta cgaaaaacaa
    25441 aaccaatcca aacaactggt gagcttgtcg aaatcattaa acaagtcatc ccagctcccg
    25501 caagaagaaa aggcgggcat cctgcaaaaa gaatttttca agctgtgcgg attgcagtaa
    25561 atgacgaact cggtgccgaa gaggcctctt tagaacaggc aatccggtta ttgaaagtca
    25621 atggaagaat cagcgtaatc acattccatt cattggaaga cagaattgtc aaaagtatgt
    25681 ttaaagaata tagtacggta caagatctac caccgggact tcctgtcata cctgacgaat
    25741 tccaaccaga actgaaagtc attactcgta aaccaatcct tccttcagag gaagagctaa
    25801 cagaaaacaa tcgatcacgc agtgcaaagt tacgtattgc agaaaaaata agaatgaaag
    25861 aggagtagaa tgatggcaga attaaagaaa atgcaagatt acgaatatga tttacctgaa
    25921 ttggtggatc agccggaaca acaaatgcct gattcttctt cgaatcaaga aatcattctt
    25981 ccacaatcac cggcaaaacg attaaagcag atttctgctt tggaaaaaat tgctgtggca
    26041 agcgtggcga ttgctattgt tgctttatgt gttttaacag ttatgctgcg cacaaatatc
    26101 agcggagctg aaaaagaaat cacgtcaata caaacagaaa tcaatgataa aaagcaagaa
    26161 aaaacgagtt tagaacaaga aaaaaacgaa ctttctcgta ctgaacggat taaacaaatc
    26221 gcagaagaaa aaggattgtc tattaatgac gacaatttaa ggaaagtgaa gtaaatgagt
    26281 cttaaaaata aaattcggcg gtttatggaa aagaaaaatc taaatccgat gaacaatcgc
    26341 aaaaaagtag gtatcatttt atttgctacg agtattggat tgttcttttt atttgccgtc
    26401 cgtttctcgt atatcgttat tggaggtcat gttgctggta cctcattagc tgaaaaaaca
    26461 aaacagttgt accaaggaag tgaagtagta aaagcaaaaa gaggaacgat ttatgatcgt
    26521 aacggtgtgg cgctcgcaga agatgcctcc tcttattcaa ttaaagcgat actctcaaaa
    26581 acatatacct ctggggataa aaaactgtat gttgaagaaa agaattttga taaaatagca
    26641 gaaatcctgc ataagaatct ttctatagac aaaaaagatg cactgaatat tttagaggac
    26701 ggcgcaaaaa aagagctgta tcaagttgaa tttggtagct atgggaaaaa tatctcccaa
    26761 gaaaccaagc aaaacatcga agcggacatg aaaaaagaag gtgtcgctgg tctttatttt
    26821 gtcgaccatc aggctcggat gtatccaaat ggcgtctttt cttctcattt tattggatac
    26881 gcagttccag acaaagatga gaatggctta gttggtaaac taggtttgga gtctgcttac
    26941 aatgatatct tgagcggtaa agatggtaag attatttatc aaaaagataa ttttcaaaat
    27001 cctctgccag gtacagtggc agaagaagaa aaagctgtcg acggacaaga tatctacacg
    27061 actttagaca gcagactaca aagttattta gaaacactga tggatcaagt aaatgaagaa
    27121 tatcagccag aagaattgac agcagtcctg atgaaagcaa aaaccggaga aatcttggca
    27181 atgggacaac gacctacttt caatcctgaa acaatggaag gattgactgg aaaagatgct
    27241 atctggcgta atttcttggt acaagatagt tatgaaccag gatcaaccat gaaagttttc
    27301 actactgcag ctgctatcga agaaggagaa ttcaacgaga atgaaacctt ccaatctgga
    27361 aaaatccaag tagaagatgc tacgatcaat gaccatgact ttggtgaaaa aggtgtgctg
    27421 accatgcgtc aagctctttc atggtcaagt aacgtcggga tggtcatact ggaacagcga
    27481 ttaggcggac gatggtataa ctatttacaa aaactgggat ttggacaaag tactcattct
    27541 ggattagatg atgaagtaaa cggtgcttta cctacattaa atattgtcga ccgagcaatg
    27601 agtgcttatg gacaagcggt aggtgtgacg aatttccaaa tgatgaaagc tttcacttct
    27661 attgcgaata acggtacaat gattcaacct cgatatatca gcaaagtggt ggatccccaa
    27721 actggagaag agcgaacaac tcaaacagaa gttctagggc aacctttttc aaaagaaaca
    27781 acagaaaaag tccgtgaata tatgagagat gttgtcgaaa gcgaaaacta cggtagtgct
    27841 tatggtgtat atagtgtacc tggatacaat gtatcggcaa aaaacggaac agcccaaatc
    27901 gcttctgata cagggggata tcaaacagga gatacagcct acctgtattc gattgtggag
    27961 atggttcctt ctgaagatcc agactatgtc ttgtatctaa caatgaaaca ccctaagaca
    28021 tatgatcgaa tggcattggc taaaatcgca aatccattga tgaaacgggc aatggatttt
    28081 aaagaaactg aagaagattc agatacagaa acaaaaacag aaaaagtatc cgtagccgat
    28141 tatcgtaatc ttgaagcgga cgttgcagca gctgatgctc aaaaaagtgg actgcaacct
    28201 gtagtgatcg gtaatggtaa aaaagtacaa aaacaatcta ctgccaacgg tgatcagttg
    28261 atctctggag aaaaactgat cctatatact ggtggtgata agctgatgcc tgacgtaaca
    28321 ggatggtcaa aagcagatat catgaagctg gggaaaatcc tcggtattga agttagtttt
    28381 gatggagatg gttattgtgt caaacaagaa cttgctccat atgaaaaaat cactgaagac
    28441 aaacttagtt ttactttaga agaataagaa gagaaatagg agagaaaaga gcatggaatg
    28501 gacacaagca ttgattccca ttgtcagcag ttgtgcaatg acaattgccg caatgccgct
    28561 atttattggt tattttcaaa tgaaaaagca aggtcaggcg attcgtgagg agggaccaaa
    28621 gtggcacaat agcaaagccg gaactcctac aatgggcggc ttagtgtttc tgatcggctc
    28681 tattttgaca ggaatttggg ttggagcttg gcaaaaacag ctaactccta ctcttttcat
    28741 cttgcttttt gtattagcgt tatacggagt aatcggcttt cttgatgatt ttataaaaat
    28801 attcaagaaa agaaacatgg gactgaactc gaagcagaaa ttgattggac agattctagg
    28861 gggaatcatc ttttatcttg tttaccgttc tgaaggttat ccaggtgtat tgaatttctt
    28921 cggactagat ctacctttag gaatcattta tggtattttc gctctcttct ggcttgttgg
    28981 attttcaaat gcagtaaatc tgacagatgg catcgatgga ttagttgctg gcttaggaag
    29041 tatctctttt gcagcttacg gtctaattgc ttggcatcag catcagtatg atgtattaat
    29101 catttgctta agtgttttag gaggtttact gggtttcttc gtctacaaca gaaaacctgc
    29161 caagattttt atgggagatg tcggttcatt agcgttgggc ggacttttag cggcaatttc
    29221 tataatgcta aaccaagaat ggacattgct tttgattggt ttgatctatg ttatggaaac
    29281 agcaagtgtg atgcttcaag ttacttcctt taagcttaca ggaaaacgaa tttttaaaat
    29341 gtctccaatt catcatcact ttgaaatgtg cggatggtcg gagtggaaga ttgatacagt
    29401 tttttggttg atcagcattg tgacttcgtt gatcacatta tggatcgttt ggtaggagat
    29461 gacgggaaat gaaaaaaata tcaacatatg aaaataagaa agtgcttgtc ttaggactcg
    29521 caaaaagcgg agtaagtgcg gccaaattat tacatgaatt gggcgcactt gtcaccgtta
    29581 atgatggaaa accttttgac gaaaatccgg aagcacaaga gctgctttct ttagggataa
    29641 aagtaataac aggtagtcat ccaatcgaat tgttggatga agaattttca ttgatggtca
    29701 aaaatccagg gattccctac agtcatcctt tagtagcaaa agcccaagaa atgggtattc
    29761 cggtaattac ggaagttgag ctagcttacg aagtagcgga atgcccaatt attgggatta
    29821 caggaacgaa cggaaaaaca acgacaacaa cgatgacagg actactactg aatgcaggag
    29881 cagatcaagg aattgcacgt ttagcgggta atattgggta tccagcaagt ggtgtagcgc
    29941 aagaagcaaa atcagaagat aaaattgtga tggaattgtc tagcttccaa ctcatgggaa
    30001 tcacagattt ccgccctcat atagcggtga ttaccaatat ctatgaagca catattgatt
    30061 atcatggtac aagaaaagaa tatgtcaaag ctaaatggaa ccttcaaaaa aacatgacag
    30121 aaaaagatta tctgatcctt aactggaatc agtcagaact acaagaatta gcgcaaagaa
    30181 caaaagcacg cgtactgcct ttctcgacaa aagaagtgtt ggaagacggt gtgtatgctg
    30241 acgattactc aatatactat aaaaaagaaa aaataatgga aatatctgaa cttggcgtac
    30301 caggtaagca taatgtagaa aatgcactag ctgctatttc tgtagcaaaa ttgtatggta
    30361 tatcaaatga agctatacga gaaacgttgc attttttcca tggcgtacca catcgtacgc
    30421 aatatgtagg agaaatccaa ggcagaaaat tttataatga ttcaaaagca accaatattt
    30481 tagcgactaa aatggctcta agtggatttg aaacgtctaa ggtcgtgtta ttggctggcg
    30541 gattagacag aggaaataca tttgatgaat taattccttc attaaaagga atcaaagcaa
    30601 tggtcgtatt tggccaaaca aaagaaaagt tgatggatgc tggaaaaaaa gcaggcatcg
    30661 agacaatagt aacagccgat tctgtagaac aagcagttcc tttagcatta gaaaacagta
    30721 cagatggtga tgtagtttta ttatcaccag cgaatgccag ctgggatcaa tacccaaatt
    30781 ttgaaactag aggaaacaga ttcatggaag ctgtgaaccg attaaaatag aaaaagtaga
    30841 agcgagcgat aaaaaatgaa aattttggtt acaggcggag gaacaggggg acatatttat
    30901 ccagctcttg cctttgtcaa ttatgtaaag actcaagaac cgaatgctga attcatgtat
    30961 gtgggagcaa aacgaggtct agaaaacaaa atcgtccctg atacaggaat gcctttccat
    31021 accttggaga ttcaaggatt taaacgaaaa atttccatgc ataacatcaa gacggtccga
    31081 ttgtttctta agagtatccg tcaagcaaaa aaaatcctca gtgattttca accagatatc
    31141 gttatcggta ctggaggata tgtttccgga gcggtagtgt acgctgcagc caaaatgggt
    31201 attccaacag ttatccatga acaaaacagt gtaccaggtg ttaccaataa atttttaagc
    31261 cgatatgtgg atcggatcgc tctttcattt aaagatgcgg cttccttttt tcctgaaaac
    31321 aaagcagttc ttgtaggtaa tcctcgagca caagaagtag cagatactaa aaaatcagat
    31381 attttgaaaa catttgattt ggatccagaa aagaaaacag tcttgatttt tggcggtagt
    31441 caaggagcac ttaaaatcaa tcaagcagta tcagtttttt taagtgaata tattccttct
    31501 tcttaccaag ttttgtatgc ttcaggagat agatattatg aagagatcct aaataaaata
    31561 aatcctgaaa tacaaaatat tagtgtccag ccttatatca aaaatatggc agaggtcatg
    31621 gcttccagtg atctgctggt ggggcgtgct ggtgcaactt cgatcgcaga attcacagct
    31681 ctggggttac cggcagtgtt ggttcccagc ccatatgtta cgaatgatca tcagactaaa
    31741 aatgcaatga gtcttgttca cgcaggagca gcaaaaatga ttgcagataa tgaactaaca
    31801 ggtgaatcac taagtcagac agtcaacgaa atcatgggtg atgaagaatt gcaaaagcaa
    31861 atgtgccgag cttctaaaga acaaggaatc cctgatgctt caaaacgtct ttatgactta
    31921 gtaaaacaaa taatcaaaaa ataaaggcgg tggtcttagt gagtaagaaa aaagaaccaa
    31981 aaaaattgtc tcatccgtct tctgatgaaa acaatttgac tccttggcaa aaggctaacc
    32041 gaaaatatct ggcagaacaa gagatgaaaa accaagagcc aaaaaaagaa gacataacat
    32101 tagatacacc cctttcagat tctgaagaag aaaaaacggt tcaagaggaa cagcaagaag
    32161 aaagccagtc cgagaaaaaa atcgaatttt ttgaagagat caagcaaaag aaaaacggtg
    32221 gaccatataa cggttctttt ttgaaccggc tgcccaattt aaaaagtcaa cggcacaaag
    32281 tactatttcg tcggttagct ttgatcatca ctgtattggg tataccgctg atttttttga
    32341 tctattatgt ttctccttat agcaaattac aagcagttgc agtatcaggc aataagacag
    32401 tcaacagcca agaaatcatc tctgatacaa aattgtcatt aggagaaaat gtttggggac
    32461 agtattttca tcgatctact tatatcgatc gccttaaaaa agcacagcca agaatcgaaa
    32521 cagcaaatat acatttcaaa gggatgaatg aattcgattt ggatgtaaca gaatataaag
    32581 agattgcttt gatcgcaaaa aacaatcaat actacccagt gattgaaaat ggaacagtat
    32641 tagatgaaaa agtagcaaat cctacaaaaa atctccctat cttggaagag ttcaaagact
    32701 ctgcaaaaat caaagaatta acaaagcaat acaatcagtt atcatcagaa ttacaaaaag
    32761 caatttctga aatcaagtac acgccaagag caagcaataa aaatctgatc caattgaata
    32821 tgaatgacgg caatcaagtt attgtaaata tcaataatct tgcgaaccaa atgaaatatt
    32881 attcgcaagt agccaaagat atggatgaaa aaggtgtgat cgacatggaa gttggtatct
    32941 tctcgcatcc ttacggtagc actcaagaaa cggaagaatc gggccaatcg gaagaaacaa
    33001 ctgaaagtga agaaaatgcg gatcaaacaa acgaatcaag tgaagaggca gaaacgcaaa
    33061 aagcacaaac acctggatcg tctagcacaa acggatcatc tgattcaaat gattcaagtg
    33121 aaacaagtca aatagaagat aatccaagta caactccgtc aagtttaaat gaaaattgat
    33181 aaaacaactt taaaaagtaa gaatgtactt tctaaaaacc agtaatttat ggtagaattg
    33241 gaagcagtga atatatctat taataagtat attttaaaac ggacgctagt tgttcgcttg
    33301 tatatggaat agtaagtagg agggaatccc attcatggca aaaacaggaa tgtatgtagg
    33361 ccttgatatt ggaacgacat ctgttaaagt tgtcgtggct gaatatatcg acagccaaat
    33421 gaatattatt ggagtaggaa acgcaaaatc agaagggatt aaccgaggca ttatcgttga
    33481 tatcgataaa acggtccaag cgatacaacg agcagtacga caagcagaag aaaaagcagg
    33541 tatccagatc aaaggcgtaa gtgtcggttt gccagcaaac atgcttgaag tagaaaattg
    33601 ccagggaatg attgcagtta acggagattc aaaggaaatc accgatgaag atgtcagaaa
    33661 tgtggcttca gctgcccttg ttcgttcaat acctcctgaa cgtcaaatcg tgtcaatatt
    33721 gccacaagat tttactgtcg atgggttcga gggaatcaaa gatcctcgtg gaatgatcgg
    33781 cgtaagatta gaaatgtacg gtctgttgtt tacaggacct aaaacgatca tccataacat
    33841 acgtaaatgc gtggaaaatg ccggattaat cgtcaacgaa atggtgatta ctcctttagc
    33901 gctgactgaa tcaattcttt ctgacggaga aaaagatttt ggtacgatcg tcattgatat
    33961 gggtggcggc caaacaacaa cggctgttat gcatgataag caattgaagt ttaccaatct
    34021 tgaccaagaa ggtggcgaat ttgtcactaa agatatctct attgtattaa atacgtcatt
    34081 taacaatgca gaagcgttga aaataaacta cggggatgca tacccagaac ggacatcacc
    34141 agatgaagaa tttccagtag atgtgattgg tcagtcagaa ccagtgaaag tcgatgaacg
    34201 ctacttgtca gaaattattt cagctcgaat ggaacagatt ttcaacaaag caaaagatgc
    34261 attggaccaa atcgaagcat tagagttgcc aggtggcgtc gtattgacag gcggagctgc
    34321 tagtcttcct ggtgttgtgg acttggcaca agaaatcttc ggcgtaaatg taaaattgta
    34381 tgttccaaat cacatggggc tgagaaaccc ggtatttaca aatgtaataa gtatcgtgga
    34441 ttattcagca aacttgagtg aagtgtatca attggcaaaa attgctgtta cgggtgaaac
    34501 ctcagcagct cgtcaaatgg tagttgaaca agaaacaaca aacacatatg agagctatga
    34561 agcacctgaa gaaacaatct acgatgagcc ggaaccaaaa gagtccggag aaaatgtaaa
    34621 aaataaaatc aagggtttct ttacaaatat ttttgattaa ttcatcctgg gaggaataag
    34681 atatggaatt ctcaattgat aataatatca atgacggcgc agtaatcaaa gtcatcggag
    34741 tcggtggtgg cggtggaaat gctgtaaacc gaatgattga agaaaacgtt aaaggtgttg
    34801 aattcattac agccaacaca gacgtgcaag cattaaaaaa ttcaaaagct gaaacagtta
    34861 tccaattagg ccccaaatat actcgtggtt taggtgctgg ttctcagcca gaagttggac
    34921 aaaaagcggc agaagaaagt gaacaatcct tacgcgaagc tttagacggt gcagatatga
    34981 ttttcatcac tgcaggaatg ggtggaggaa ccggtacagg tgctgcacca atcgttgcag
    35041 gtatcgcgaa agaattgggc gcattgactg ttggtgtcgt gacacgtcca ttcacttttg
    35101 aaggaccgaa acgtggccgt tttgctgcag aaggtatcgc tcgtttgaaa gaaaatgtgg
    35161 atacattatt aattatctca aataaccgtt tattagaagt tgtagataaa aagacaccga
    35221 tgcttgaagc tttccgtgaa gcagataatg ttttacgaca aggtgtacaa ggtatctctg
    35281 atttgatcac agcgccaggt tacgttaacc ttgactttgc tgacgtgaaa acagtgatgg
    35341 aaaaccaagg tacagcattg atggggatcg gtgtggctag tggagaagat cgcgtagtag
    35401 aagcaacgaa aaaagcaatc tcctctccat tgcttgaaac atctattgat ggagctgaac
    35461 aagtacttct taacatcaca ggcggtttgg atatgacatt gttcgaagca caagatgctt
    35521 cagatatcgt agcaaatgca gcaactggcg atgtaaatat cattctaggt acatctatca
    35581 atgaagaaat gggcgatgaa atccgtgtga cggtcatcgc aacaggtatc gatgaatcaa
    35641 aaaaggaacg caagtctagc cgaccagcta gacaagcaca aatgcaatca cctgctcaaa
    35701 agactgtatt agatatggat caagctaaac caacttcttc agaagaagaa aacagttttg
    35761 gcgattggga tatcagacgt gaacaaaatg ttcgtcctag agttgatgat tccaattttg
    35821 acaatatcga aaaaaaagaa tttgatacat tcaaccgcga agaaacaaaa tctcatggcg
    35881 atgatgaatt aaatacgcca ccgtttttcc gtcgtaaaaa ataaaggaga ggaagagcac
    35941 atgattgctg acaacttgca gaaaatcaag caagaaattc aggattcgtg tgctcttgtt
    36001 catcgtgatc cgaaagaagt tacattagta gctgtaacaa aatctgttga atccgctcaa
    36061 gctgaagaat tggttagagc tggcgttatg aattgtgcag aaaaccgagt agataaatta
    36121 ttagaaaaga aacaagattt atttgatttt cctgaaataa agtggcattt aattggtaat
    36181 ttacagcgca gaaaggtaaa attgattgta aatgaaattg attactttca tgcgttggac
    36241 agtttaagat tagcagaaga aatccagaag cggttagaga aagaattatc ctgttttatt
    36301 gaagtaaatg tttctggtga ggaatcaaaa catggaattt caccagatga gcttttaccg
    36361 tttgtggaat ctttagctgc ctgcacaaaa atcaaggttg ttgggttgat gactatggcg
    36421 cctaaagaag cttctgatga gcaaatccat aaagtgtttg gtacgctgaa agaattgcgt
    36481 gatcttgttc aagcaaaagg ttatgcccat gcaccatgta gtgaattaag tatggggatg
    36541 agtcaagact ttcaaattgc gattgaagaa ggtgcaacat ttatccgtgt aggaacagcg
    36601 ctattcaaag acgaataaag gaggaacaaa aatgccgctt ttaaacagag gagcaattgc
    36661 taattttttc ggattagctg atgaggaaga gtacgaatac caagattatc ctgaaacaac
    36721 aaaaaaggct gtacagccaa ctaaaaaaaa tacaacacag aatcaatcaa ctcattctgt
    36781 atctgctgcg aatactcatg aaaaacaaaa aacggcgact tcacgtcctg taacgcatga
    36841 aagtattcag ccggctgaaa agaaaaaagt aattgaacta catcaatcac ctacaaaaac
    36901 tgtagcgaaa gaaagaacag cttcaagaac ctctacacaa cctaaagcaa atcttgctgg
    36961 aaaaatcact gtaatggaac caagagctta ttctgaagct atggcgattg ctaaacgaat
    37021 cattgctgga gaagcaacct tagtgaattt tcatttagta gaagaaaaac aagcgagaag
    37081 aatcgttgat tttctaacag gaacagttta tgcactagat ggagatatca aacgtgttgg
    37141 agatgagatt tttctttgta caccgactgg gatggagatt gacagttcaa ccgctcaatc
    37201 ttttgcagat gcgaatttgt ttgatctgta agaggaggaa ctacgattta tactattgca
    37261 ctattacttc atttacttca acgggcagcc tatttctata gcattatctt gatggtatat
    37321 gcattgctct cttggtttcc aggcgggtat caatcagggc taggacgatt attagctaag
    37381 atctgcgaac cgtatatcag tttgttcgat cgtctgcctt tgaggattgg acccttggat
    37441 ttcacaattt taggggcaat cctgatcttg aacttagccg tacaaggttt agcacagatc
    37501 gttatatgga taactgtcca tttagtttat tgattgaagc aacaaaatct ttattctcag
    37561 aatagatagg aggggaaaat tttggatgca aatgtatacc agcactttcg ggcagatgaa
    37621 cgtacattta tcgattcggt aggcgattgg attgaacagg ttcaaggaca atatgcaccc
    37681 tatctgaccg aatttcttga cccaagacaa tcctatattt tagaaacact gattcgacaa
    37741 agcagtgatt tgcgtttcat gttttatggc ggatatgaac aggcagaaag aaaaagatgt
    37801 ttgattttcc ccgattatta cgaacctgta gaggaagatt ttgaaacagc attgtatgaa
    37861 atccattatc ctagcaaatt tgccagccta agtcatggga aaatccttgg aactttagtg
    37921 ggaacgggga tcaaacgctc ttactttggc gatatcatct cggacggtga tcgctggcaa
    37981 gtatttattg caaatgaggt ggatcatttt gttgctgcac aagtaacaaa gattggaaag
    38041 gttgctgtac gcttggaacc aatcaaatac acagaaatga ttatgccgaa agatagctgg
    38101 acacaagaaa gaggaacagt tagttccatg cgactagaca gtgtgatctc tgagatttat
    38161 aatatttcca gacagcggtc caagcagctg atcgaagctg gtaaagtaaa agtgaactgg
    38221 acagaaaata caagacctga ttttgtactt gaactattag atattatttc agtcagaggt
    38281 tatggcagag tccagatcca agaacttgag gggaagacga aaaaagaaaa gtacagggta
    38341 ctgttcggtg tattacgtaa ataaaataaa aaggtggttt actcatggct ttaactccat
    38401 tagatatcca aaataagaca ttcccgacaa aaatgcgggg atataatcaa gacgaagtag
    38461 atgatttctt agatttagtt gttcgtgact atgaggaatt gactcaaaga aatcgagaat
    38521 tagaaaaagc agtcaaacat tctgaagaaa aacttgagta cttcaatgaa ttaaaagatg
    38581 ctttgaacca atcaatcatc gtagctcaag atacggctga taaagtaaaa acaagtgcaa
    38641 gcaaggaatc cgaagtgatc gttacttctg cacaaaacaa agcagatgag ttagttgcga
    38701 acgctgaaaa acgtgcacat caattaacaa ctgatgcaga agaaaaagca agaaaaatct
    38761 tgacagatgc aacagaaaaa gcaagacagc ttgcgacaga aactgaagat cttaagaaaa
    38821 aaacacgagt attccatcaa cgtatcagct tgatgcttga gtctcaatta gaacaagtga
    38881 aaagtccgga atgggatgaa attttacaac cattctccag ctacgtgact gacagccatg
    38941 aagtgatcaa agaagtttta gcaaaacagc ttgacaatga aaatgatact gatgtaaact
    39001 cagacacagg agtcatagaa gcacctatga cggcatttga tacacaggct attgatatct
    39061 cgatcgtgcc tggtgaaaat gaaaacgagt agaacagaaa agcactttgt tattcttaaa
    39121 gcgagccgat gagagtggaa gttcggtaag acactgcatg gtgaaagatc ctctacttga
    39181 gacctgcttt tccgaaatta gagtaagaag cagcggctga cctcgttatt ggtcacagag
    39241 agtttgcact agatagatgc aactaaattt gggtggtacc acggcatgtt cgtcccttgc
    39301 ggctgaacat gcttttttgt gtccggaaaa caaataaagg agtgtatggt tgaatgaaaa
    39361 tgaaagaaac cttgcaatta ggaaaaacaa aattccctat gcgtggtaat ttgccaaacc
    39421 gagaaggcga gtggcaaaaa gaatgggaag aaaatcgaat ctaccaaaaa agacaagagt
    39481 tgaacgaagg aaaacctagc tttgttcttc atgatggacc tccatatgcc aatggtaata
    39541 tccatttagg acattcattg aataaaatca gtaaagacat tatcattcgt tcaaaatcaa
    39601 tgtctggcta tcgtgcacca tatgttccag gatgggatac acatggattg ccgattgaac
    39661 aagtattggc taataaaggc gtgaaacgta aagaaatgtc catggcagaa tacttggaaa
    39721 aatgtcgtga atatgcactt tcccaagtag ataagcaacg agcagatttc aaacgcctag
    39781 gagttgcagg agattgggaa catccttatg ttacattaga tccttcttat gaagctgcac
    39841 aagtacgcgt atttggtaaa atggctgaaa aaggctatat ttacaaagga ttgaaaccaa
    39901 tttactggtc tccgtcaagt gagtcttctt tagcggaagc tgaaattgaa tataaagatg
    39961 taaaatctcc atctattttt gtagctttca atgtcaaaga cggcaaagat attcttgatg
    40021 aagatacagc ttttgtcatt tggacgacaa caccatggac attgccagca aaccaaggta
    40081 ttgctgtaaa tccaaaattt acgtatgtat tggtggaagc cgatggccgt aagtttgtcg
    40141 tagcgaaaga tttgctagaa acagtccaat cagaaatcgg ttgggaaaat gtaacagttc
    40201 taaaagaatt tgctggacaa gaaatggaat atatgactgc aagtcatcca ttctatgaac
    40261 gtgaatcact tgtgatttta ggcgatcatg ttactttaga cgctggtact ggattagtcc
    40321 atactgctcc aggacatggg gaagatgact atatcgctgg tagcaaatat aaactatctg
    40381 tagtgtcacc tgtagactca aaaggattgt ttacagaaga agcacctgga tttgaaggta
    40441 tcttttatga caaagcaaat cctatgatca ctgatctatt aaaagaaaaa ggagctttgc
    40501 tgaagctaga tttctttact catagttatc ctcacgattg gcggacgaaa aagcctgtga
    40561 tctatcgggc aactccacaa tggtttgcat ctatcgataa attccgtcag aatattttgg
    40621 atgaagtaga aaaagtagag tgggttatcc catggggtaa aacacgtttg tataatatga
    40681 tccgtgaccg cggcgattgg gtaatttccc gtcagcgtgc atggggagta ccacttccga
    40741 ttttctatgc tgaaaatgga gaagcaatca tcacaccaga aacaatcgaa catgtggcaa
    40801 aattatttgc tgaacatgga tcaattatct ggtttgaaaa agaagcgaaa gaacttcttc
    40861 ctgaaggatt tacccaccca ggttctccta atggcgaatt tacaaaagaa aaagatatca
    40921 tggatgtctg gtttgattct ggttcttctc atgaagctgt tttacgtcag cgaccagaat
    40981 tatcattccc agcagatatg tatttagaag gatcagacca atatcgtggc tggttcaact
    41041 caagtatcac gactagtgtg gctatcaatg gtgtagcacc atacaaatca gttatttctc
    41101 aaggatttgc tttagatggt gaaggccgta agatgagtaa atctttagga aacatcattg
    41161 caccagaaaa agtgatcaaa caatttggtg cagatatcct tcgcttatgg gtatctagtg
    41221 tagattatga agcagatgtt cgtgtttcta tggatatctt aagtcaagtg gcagaagtat
    41281 atcgtaaaat cagaaatacg atgcgtttct tattagcgaa tacagaagat ttcgatccta
    41341 aaaaagatac tgtttcgtac aatgatttac gttctgtaga caaatatatg accgttcgat
    41401 tgaaccaagt aatcaaagaa atccgagaag aagggtacga caaatataac ttcatgcata
    41461 tttaccgtac agttatgaac ttcttgacgg ttgatctatc gtcattctac ctggattttg
    41521 ctaaagatgt agtttatatt gaagcagaag atgattatca acgtcgttgc atgcaaactg
    41581 ttttctatca aacagctgta gctttgacaa aattattgac tcctatcatt ccgcatacag
    41641 cagaagaaat ctggtcattc ctgaaagaag aagaggagta cgttcagtta agcgagtttc
    41701 ctggttatga agagtttgca aaccaagaag aattgatgga tacctggact gctttcatgg
    41761 acttccgtga taatgtctta aaagcattgg aagaagcaag aaacagcaaa ttgattggta
    41821 aatctttgga agcaaaacta actgtttatc caaaacaaca agttcgtgaa atgttaaaag
    41881 ctttggatgc agatattgca caattattga tcgtttcacc agactacttt gaagttatgg
    41941 cagcagatga acaagtgcca gataacgcta tggtatttga tgatgttgct attacagtgg
    42001 aaaaagcaga cggggaaact tgcgatcgct gtcgccaagt tcgtaaagat gtaggagtag
    42061 atgaaaaact tcctcatcta tgcggtcgtt gtgcaaaaat cgttgaagac ttctatcctg
    42121 aagctgtagc tgaaggattc gaagaaaagt aaaagggaaa agggggtgtg acatgttctt
    42181 tgtcacaccc ccttttgtta tcaatcgttt aattttccac gttcacgata ccagttatct
    42241 ggttgccaga tccattcgaa tccgtctttt tcaagtagtt cgaaagctgc tttaggtccc
    42301 attgatccag ctttgtagtt gggaaaatcc ggttgtgttt ggtcccaagc ttttcgaatg
    42361 acatccacga tatgccaaga ttgagcgact tcttcccaat gagagaagtt cgttccatca
    42421 ccattcaacg catctaatag taatttttcg taagcttctg ggctattttc aaccatttct
    42481 gcactatttc taaattccaa tttgattggt tccgtttcaa atccttgccc tacttctttc
    42541 ccattcaagg ataaagaaaa tccttcagtt ggctgaatat agatcgttag aacattaggt
    42601 ggaagtgtcg tatcgtcaca tggttcatcg acagatgttt tgaatacatt aacaggaact
    42661 tgtttgaata cgatgttgat ccgagtccct ttttcagtca aacgtttgcc tgtacgtaca
    42721 tagaaaggaa cacctgacca acggaagtta tcgatagtga atttgcctgc gacgaatgtt
    42781 tcagtctctg attgagcatc cactttgtct tcttcgcgat agcccttgaa ttgtacacca
    42841 tccaattcac cttgagcata ttgcccgcga acgaagtttt ctaacacttc ttcttcgtta
    42901 taaatgcgga tagctttcaa tgctttgatc ttttctgtac ggatttcttt ttcggaaaat
    42961 gctactggcg gttccattgc aagtaatgct acaacctgta aaatatgatt ttgcaccatg
    43021 tctttaagtg cgccactatg atcgtagtag cccccgcgat cttctacacc gaggttttcg
    43081 ccaaaagtaa tttggatatt gtcgatatag cgattgttcc attgagcctc aaaaatatta
    43141 ttggcaaaac ggatagctga gatgttttgg atcatttctt ttcctaaata atgatcaatc
    43201 ctgaaaatat cttgttctgg gaatacttct ctgatttgct ggttcaattc aaaagcagac
    43261 tggtaatcag aaccgaacgg tttttctatg atcaaacgat tgaagccatt ttttgtcata
    43321 atattttgtg atttcaaatg agaaacgatc gttccaaaaa attgaggtgc catagccaaa
    43381 taatagacac ggttaccgtt taattgatag tgtccatcta atcggtcaga caattctttt
    43441 aatgtctgat aatgcgttga atcatttaca tcatgggatt gatagtaaaa atggcttgca
    43501 aaggtagttg cttcttcatc tgaaggattc aatgcacgga ttgtttcttt gacaacttca
    43561 cggtagtgct catcgctcca gggacggcga gcggttccga ttacagcaaa atgctcgcct
    43621 aaatttcctt ttctgtaaag gcggaacaaa gacgggtaca atttacgctg ggctaggtca
    43681 cctgtaccgc cgaatatcgt aaacaataca tttttttcat tagacatgtg gatacactcc
    43741 tttatatttt atcgacaaaa atcgtgctgg ccgccttaaa gctgacgggg attttctttc
    43801 tatcattttg gatagtgatc ggtccttcat aagctgcgat gtcaacgatc tcatattctt
    43861 cgttgatatt caggtcaata gtgactaagt aatccaacaa ttctttttca tcaagcactc
    43921 gtgcgatacg cacttttgta ccgacaggat aggactccaa tgtttgtcgg cgttcttcat
    43981 ggacaacttc ctctttttta ggaatcgatc ctccatgagg acaatgttca ggatgattca
    44041 aatagttttc taaatgatct gcaagatgtt ccgaagtcac atgttccaat acttcagcat
    44101 catcatggac ttcattccag ctatagtcta aatgttcaac taaaaaaact tcccataagc
    44161 gatgtttgcg aatcagactg ctagcttttt gcagtccgct tgcagtcaat tgtacacctt
    44221 gataagggga atgttctaca agctcttctt tcacaagttt agaaatcatt tcactgacag
    44281 atgcagctga gacatctaga ctagatacga tttgtttatt gttgactttc gttgtatctc
    44341 cacctagttc taaaatgatt ttcaaataat cttctcgatt tggtgtcata ttcgatcatc
    44401 ctttcatggt aaaaaaccat cattagtaag tgtaacaaaa aaaacgttaa ttgactattt
    44461 ttctaaataa aaaacaaagg atttttagac gtttttacaa caaaaaacga aagatgatag
    44521 agaagactca ttctttcgtt tataaaggat atatatatac attgggaggg atctattttt
    44581 cagataacaa cttatatgaa gtgtctgcag attcgtcata aagagtttgt tcgggataag
    44641 aaaagataag ataaaaacca tactgatcac gtacgactaa acgatattca tctatttcta
    44701 ccacagatgt gccaagagac tgaccgttga tcaaaaacgt gtaatcagat gtaatctgta
    44761 attgttgttt ttgctggcta tcactaaaga accatgtacc ttgatacttt gagagtcctt
    44821 ctgacttttc cgcttgtttt ttctttttca agtaaataaa acttgagaga acggtaatga
    44881 gtgaaccaag aaaatagatc atttttttta tcaccaaatc actcctttat gagaaaataa
    44941 tcgcatagac atattctttc ttctttattt cattgtaccg aataaaagct aaaatttctc
    45001 taagactttc gactggtttt tttactgttt ttctacaaat tatgatagga tacatagtag
    45061 gaagaaaggt gacataattt gaagtatcag caagtggaaa tagctttttt tattgaccgc
    45121 ccgaatcggt ttatagcaca ttgtatgaaa aaagacggag aaataattat tgcgcatgtc
    45181 aaaaatacag gtagaggaaa agaggtcctg ataccaggcg ctgaagtggc tattgtattt
    45241 gctcctggac ctaaacggaa aaccgcctat gatctgattg ctgtaaaaaa gaagggtgac
    45301 tggtttaata tcgatagtca attgcctaat cggctggcaa ttgatggtat tttagacggc
    45361 acgatccttt taccaggatt aactggtcca tttacggtat acaagcgtga agtaacttat
    45421 cttcactcca aatttgatat ttatttagag acggtggtcg gacaaaaagc atttgttgaa
    45481 gtcaaaggta tgacacttga aaataaagcg attggtgctt ttcctgatgc gcccacactc
    45541 agaggattga aacatatagg agaattaacg tatgccgcac aagatggtta tgccgcttat
    45601 gtcttgttta ttgctcagtt cgaacatctt catctggcaa cgatccatga agaaatgcaa
    45661 ccagcactag ctgatatggt tcgacatgcg cagcaaagcg gagttcaaat cctcgcctat
    45721 aattgtcaag tatcggcaga tgaggtaact gtcaagcagg ccattccatt tgatgtagat
    45781 atcccttttg aagatcctat acaatagaaa gaaggtaaaa taatgatcaa cttaggtatt
    45841 atcggaacaa attggattac tcatcaattt gtccaagcag ctttaagcac aaaaaaatat
    45901 gaattgacag cggtatattc tagacatttg gaaaaagcac aggaatttgg ttctcattat
    45961 gaaggggata tcgaattcgc aacagatttg aagacttttt tcaatattac tcatatggac
    46021 accatttata ttgcttctcc gaatagtctt cactttagtc aagctaagca agcaattttg
    46081 gcaggaaaaa atgtgatagt tgaaaagcca gctttttcga cgccaaaaga aatggatgaa
    46141 atcatccatt tggcaaatga acacaatgtg tatttttttg aagcagcaag aaatatccat
    46201 gaatcaggtt ttaaaaagat tgccgattac ttaccattaa aagacgagat aatcggtgca
    46261 aatttcagct atatgaaata ttcttcgcgc tatgaccaag tattagatgg agaagaaccg
    46321 aatatcttct cgactcattt ctcaggtggt gcattgatgg atttaggtgt ttaccttgta
    46381 tatgcagctg ttgcttggtt tggaatgcca aaagaagtcc attatttccc aagaaagatt
    46441 tctactggtg tagatggtct tggatggggg atcttacgtt atgagaattt tgatgtatcg
    46501 atccagccag gtaaaatcgg tgattcttat ttaccgtcag agatttattt tgactcagga
    46561 acgctagtga tgaatggagt caatgcaatt gaacaagcac aatttcatga tcgcgcgcat
    46621 aaagaaatca ttgatttggc ggtaacagct gcagaaaatc caatggcaga tgaagcagag
    46681 gattttgcca atgtcatggc tcatccgaaa gatccagctt ggggattgtt gtatgaagag
    46741 tggatcgagc tagctagaaa tgtgaaccaa gtgatttatg atttaagaaa aaatggcggc
    46801 atcaaattcg atgcagataa tgaggaagat ttttaatgaa cgaatttgta caagtatttc
    46861 ccgaaaattg gcagaaaaaa tatgaggata aagggtttat ctcaccttct cttattcaac
    46921 gagcagtttt tcagccacta agtaataaac aaagtatagt agcggtttct cctacaggtt
    46981 caggtaaaac actggcttat ctttggcctc tactactaaa cgtggaacca ggtgaagcaa
    47041 gtgcgttggt catttttgct tcttctcaag aactagctat tcaagtagca gatgttgcac
    47101 gtgaatgggg aaaagacaaa gagttgaaag tccaatcttt ggtgggcgga gccaacgtca
    47161 aacgtcagat tgagggtttg aaggaaaaac cagaaatctt gattggtaca ccaggaagaa
    47221 tattggaatt gatgaaagca aaaaaaataa aagcacatca agtgaaaaca atggtatttg
    47281 atgaagcaga ccagctattt gacgctggga acagccaaat cattgatcaa attcttcatc
    47341 aagctccaac agagtatcag ctagcttttt tttcagcgac agcagatcgt tcccttgaaa
    47401 gcattgaaaa aatcactggg aaaacgtttg agacaattga cgtgacagca gaggacgatt
    47461 ctcgaaaagg actgagacat tactttttac gtgtaccgat acgaaagaaa gatgactact
    47521 tgagacgtct cacgcatata gaagattttc atggtctgat tttctttaat caactgactg
    47581 aattaggcgt aatggaagaa aaattgctgt atagaggtgt accggtagga agtctggcct
    47641 ctgatcagaa caagctactc cgaaaagcag cgctagaaca attcaaaaag aaaatggtca
    47701 gagcactttt gaccacagat gtggcggcta gaggtttaga tattacaggt cttccttatg
    47761 tagtgaatgc agaagttccg ttaagtgaag aagcttattt gcatcgttcg ggaagaactg
    47821 gtcgaatggg taatgaagga atagtaatta cactcgtaca agataataat ctaagagatt
    47881 taaaacggct tttacgtcca tcaaatataa cacttgaaga gattttttta tacggaggta
    47941 agctgcaaac tgagcagcca accaaagaag atgcagctcc tgcagtaaat aaaaagtatg
    48001 cgaaaacttt ttctggaaag gagcagaacc cagaatataa caaagaaaaa ataagaaaaa
    48061 aagttaaaaa taaatcaaaa aaagagagaa ataaaggagc aaggcgcaaa taagcgcctt
    48121 tttatgctat gattatgaaa acgggaacaa aattggttgc cagaggaggg gttatgtgaa
    48181 agaacgtcaa gaagagatct tgcagctatt gacagaaaac caacaaggat tgacagcaag
    48241 tgatgtagct gagcgcttaa ctatcgatcg aagcaatgca agccgatatt taagtgaact
    48301 atacaaggcc catcatattg taaaaaccgc tggccgacca gtcgtttact ctttgccaac
    48361 ggaaaagtca aaatctgatg aagtgcatgt cgactcttct acgcaagtga cttttgaaac
    48421 actcgttgga gaaaatgatt ctttaaaagt cagcatacag caggcaaaag ctgcgattct
    48481 ttatccaccg cgggggttgc atacgattat ttttggagaa actggtacag ggaagtcgat
    48541 gtttgctgaa tgtatgtatc attttgcaat cgattcagag atgctttctg cagatgcgcc
    48601 atttgtttct ttcaattgtg cggattatgc tcaaaatccg caactgctct ttggacacat
    48661 ctttgggatc aaaaaagggg cttacacagg agcagctcaa gatagtcccg gtctaatagc
    48721 caaagcagac ggcggtatat tgtttttaga tgagatccac cgtttaccgc cggaagggca
    48781 ggaaatgctg tttactttca ttgataaagg tatttaccgt ccgcttggag aaagtgcaca
    48841 agtacatgaa gcatctgttc agatcattgg ggcgacgact gaatcttcag aaagtttctt
    48901 atctacgttc aatcgacgga ttcctatggc aatcaccttg ccgaatcttg cttcacgttc
    48961 actggatgag cgttatgaaa tcatctctct ttttatcaaa caagaagcga atcgtttgaa
    49021 tcaacgtatc gatatcgaaa aagaagcgat tcttgccttc atgctttatg atgcggaagg
    49081 aaatatcggt caagtgaaac gagacttgaa actagtctgt gcaaaatctt ttttacatta
    49141 ccgtactcat catgagaaaa agctaatcat tcgaaaagaa gaactttccc ttcaagtaca
    49201 aaaaggatta ctgaaaatca aggaaatacc ggaacgtctc gaccgattca tggacagcaa
    49261 aagtcagtat ttgacatttg aaccaggatt tgctgatgtt gtctggtcac aagatccaga
    49321 acgaaacatg caggtctata acgatattga ggaaaaagtc acttctcttt ccgaaaccgg
    49381 tgtggagaac attgatctag agaccctcat ttcaaaagat gtggatgctt atttccagac
    49441 atatgtcaaa gaattgacta aaagttcgat tcccaaagat cttttgccag atgaaatctg
    49501 gcggttaacc aatcaattgt atgatgtggc tgaaaaacgg ttagaccgta tttataatga
    49561 aaaagctcgt tttgcttttg ctttgcatct gcaaagtaca ttggatcgtg tgagagaagg
    49621 tcatatgatc gtccacccag atttgaataa cgtacggaaa aattttaaga gggaattcca
    49681 ggtagcaatc gatctttcaa cgatcattga agaagaagaa caagttgaga ttccttttga
    49741 tgaaatcggc tttattagta tgtttctttc tatcaatcta ggagaaagtg agcctgttaa
    49801 agaaaacaaa gtagatgttc tcatattgat gcacggcaga cagactgctt caagcatgtt
    49861 ggagatggca caagaactat taggaacaaa agtagggaaa gcttttaata tgccactggt
    49921 tatggaagtt caaacaatgt atgaagaagt gttacaatat gtcaaaaact atcaagagca
    49981 tttgacgaat ggcctactct tgttaacaga tatgggatca ttaaacacct tcagtgattt
    50041 gatttatgaa gagactggca ttcggacgaa agctatcacg atgacaagta ccatgattgt
    50101 gatagaagcg atacggatgg caagtgttgg aagaagctta gaagatattt atcaaaatat
    50161 ccagctttcc tttgaatcga tcgtacgtga tcagttcaaa gcggaaaagt ctgaaacttt
    50221 acagcgtaaa aaagccgtaa tcgtgacatg ctttactggt gaaggagtag ctgccaaatt
    50281 atatcagcgt attatgccag tgattaatca aaataaggta gaactgatcc agatgcagtt
    50341 tttagaacgt gaatcattca aaaaacacat cgatgcctta ttggaagaat atgagatcaa
    50401 agcaattgcg ggaacagttg atgttgatta tcaaaatatc ccattttttt cagcctatga
    50461 cgtttttgat gatgaaaaac taaatatttt aaagcgtata gctgatgatg agatcccgac
    50521 agctaaaatt gtcgattcac ttgctggatc attaagcgat gtcttttcta ttgatgaact
    50581 gatccgttca ttgcaaaaga cagttcagca gatccagacc gatttgtcga ttatcgtgga
    50641 acctggagta gatactggaa tcgttatcca tttagccttc atgttagacg gattgatcaa
    50701 acaagaaagc catcggcaat ttgagaaact gtctattttc tcaaaaattt atcgtttgga
    50761 aatggatatc gttcgggcaa acttgatggc actagaacga aaatatcagg taaaaattcc
    50821 agaagatgat atcgcatttc ttacacaaat gtttcttgaa aataaaataa aaaaatgaac
    50881 acactaatca tactgtttac acactaaaca tctgtgtgcg cactataaaa aagagctaaa
    50941 aagcatgaaa acgctatttc agctcttttt tttattggca tgcttcttgc gttatataaa
    51001 agtgtaaaaa aataaaagga gtgttaaaaa tggcaaaaaa aacaatcatg ttagtttgtt
    51061 cagcagggat gagtacgagt ttattggtca caaaaatgca aaaagcagca gaagaaaaag
    51121 ggatggaagc cgatattttt gcggtctctg cctcagatgc agataataat ttagaatcta
    51181 agaatgtcga tgtattatta ttaggtcctc aagtacgttt catgaaagct caatttgaac
    51241 aaaaacttgc gcctaaaggt attccattag atgtcatcaa tatgcaagac tatggcatga
    51301 tgaacggaga aaaagttctt gcacaagcag aaaatctaat gaaataaatc aagtatgcag
    51361 ttgggggtat aacaacgtgg aagatcaaca aaatctagaa gccatcatgg ggttaatcat
    51421 gtttggcggt aatgcaaaga gcgatgcaat ggaagcgatt gcggctgcta aaatcggtga
    51481 ttttgagtta gcggatagga aaatcgctga tgcagaagaa tcacttatac aagcgcatca
    51541 tgcacaaaca gacatgttga cacaagaagc acaaggaaat catatgcaag tgacattatt
    51601 gactgttcac agtcaagatc acttaatgac atctattgcg tttactgatt tagcaaaaga
    51661 aatcatcgaa ctttatcgtc gcgtatacag cgaataaata caacttcttt tttgaacgga
    51721 agaagaaaaa tttttgaacg gattttttat tttgttataa ggagaactct ctttatataa
    51781 aaaacttata ggaggaagta aaaaatgaac ggactatcaa gttggatgga gaaatacatc
    51841 ctgccggttg ccgcaaaaat cggagcgcaa aaacatttag ttgctttgcg tgatgctttt
    51901 atcggcatac tgcccgcaac aatggcggga tcagtcgctg taatgatcaa tgcaattatt
    51961 cgtgatttac cgcaacagtt taacagtacc tatgctgaca gtttagcagc agacaaagga
    52021 atctttgccg tattgaatgt catcatcgga atcaatggtt ttgtctggaa cggtacacta
    52081 gcaatcgccg gattgatctt tgccttttct tgggggtata atcttgcccg agcttatgga
    52141 gtaaatgatc tagctggggg gatcgttggt ttagctacct tgattcaagg tatttccttt
    52201 ggtccagcat tatcagcagc attagatgtg aatgttcctt caaatgtcgc agatgcaatc
    52261 aatagttctg agattggtgc aacgattgca gatggcacac tgtcagttgg attatggggt
    52321 tggctaaaac tagatcattt gaatggaaac gcttatttta cagttatgat catgggagct
    52381 atttcagtca ttattttttg taaattgatg cttgccaaca ttacgatcaa aatgcctgat
    52441 tcagtaccac cagcagtatc taaagcattt gcagccattt taccagcgac gatcgcttta
    52501 tacgtcattg ctattatcaa ttatatcgta ggtcgtgtag ctgatgggca actgatcatt
    52561 gatttggtgc aaaaatatat tgcagaacca ttcctaggat tatctcaagg gttgggagca
    52621 gtattgatcg tcacagtatt tgttcaagta ttctggttct ttggtattca tggatcaaat
    52681 gtattggcac cagtgctaga gggaatctgg ggacaagcac aactaatcaa tattgatatt
    52741 ttccaaaaag gttataacgg aaaaactggt acaccagccg tgttagatgc aatcgaagat
    52801 ggaaaagctt atatgtgggt ccgcggatcg ttcgatgctt ttgcatggtt tggcggatca
    52861 ggcggaacga tcgtcttact gatagctatc ttgattttct ctaaacgtgc agattatcta
    52921 acagtagcta aattatccat tgggcctggt atcttcaata tcaacgaacc agtgatgttt
    52981 ggtctgccaa tcgtattgaa tgcaatcatg tttatcccat tcttgattgc tccagtagta
    53041 gcaacagcaa ttggatggac agctacttat cttggcctag tcgctccggt atcgcagcaa
    53101 gtcacatggg tagtaccgcc gttcttattg tcattcttag caacaggagc ggattggcgg
    53161 gcaccgatcg taacattagt atgtatggtc gtgactttct tgatttgggc accatttgtc
    53221 atctctgcaa acaaaatgga ccctgccgca ggcgaacaac aataagatga acgtgaggag
    53281 cgggacgctt taagtttcgt tcctcataat gaattttcga ggaggaactg aaatgattac
    53341 aattgccagt gcaatgcaac aagatgaaat caaagaattg ttagaaggat acaatgaaga
    53401 aggaatgact tttacattta aagaaaaaca aggaatcaag ctgttctttg aaacaagtgc
    53461 agaagatccg gaagttgcgg ctaaaaaagc aaaggaacta atcaaagcac agccttgggg
    53521 aacagtcctt tattttcaat caacagctgt ttagaaaatg gtttaaaaag ataggatggt
    53581 tgagatgata aaaaacggtt tatttttatg tagtattggt tctttgatcg tgttgtatag
    53641 tttgaatcca cattcgcaaa ggtatgattt gtatagtatg gtcacaggtc tttttctaac
    53701 aggattagga atctatttct tcatgaaggg gaaaaaaaga gaagaagcaa aggagaaaaa
    53761 taactaatgg aaatcacgaa aaaattaaat attccagcat cgttttttta cgataaagtt
    53821 atggactctg tccttttcga tgtacgtaaa gcgaccggca aatcagtaac tagaaaacag
    53881 ctgaaaaatt tggagtacgt caaacaattc tcgcaaaata accgggctcg cataaaaatc
    53941 gaagaagtca ttgaaaacca gtcttataag tttcgtacat cgacaacgaa gaatgaattc
    54001 gtcgttcatt atcaaatcaa accattggac gattcgacct gtgaggttca ttacacagaa
    54061 aaaatggagt cttatggcat gcttcaaaag ttgaatgata tgcttttagg aacaatcttg
    54121 atgtatttta aacggagacg tttcaaaaaa atgctagata tgatcgaagc aacgtattaa
    54181 atcaatgaaa catggtacta tttagttatt gaaaaatgaa gtaagctaga aggaattttt
    54241 cataggaaga tagatctttc ctgcgaatcc tcactagctt ttttgtagga aggggcagca
    54301 aaatgacaaa caaagtaaga gagctaggtg tatcttcgat tgaggaaatg tttgatttag
    54361 ccacttatgc atttaatggg gcagatacac ctgaaaggcg agaacgtttt cgtacacttg
    54421 cggagaattc ttggaattac ggattttttg atgaggagga tcggctaaca agtcaagtca
    54481 tggcgacccc ttttccagtg aatttttacg gacaagagta cctaatggcc ggcattggct
    54541 atgttgcttc ttatccagaa gcaaggggac aaggcgggat caatcagatc atggaaaaga
    54601 tccttgaaga ttgccgtgat cgtaaagtaa gcttatctta tctggcgcca ttctcctatc
    54661 ccttctatcg gcgctatggc tatgaacaat catttgataa aatcagttat caactaaaca
    54721 gtcgtgatgt gccgtttatc aaaaaaaaat ctggaaaaat aaagcgtatg gattttgaag
    54781 atgcaaaagc tgcaatcaga agaatttatg aacagatgcc ggaaaaccaa cgagctggtc
    54841 tgaagcgcga agactggtgg tatacgtata aattcaagca aaaaggcaat taccagtttg
    54901 ctttgcattt tgatgataat gaggaagcaa cgggatatat cgtttatcag cttgcaacgt
    54961 cttcctttat tattgcggag tggggatttt tggatcatga tgctttttgc agccttgttc
    55021 gcttcgtaag ttctcacaat ggtgcttttg aaacatttga atattcatgc ggctacggcg
    55081 gtaatgatca aaattatcta ttagaaaatc catttgcttc tgtaatgatc acaccttata
    55141 tgatggcgag gatcgtagat atttctttgt ttttggaaaa ttatccattt aaaaagagac
    55201 aagctgcatt tgccctacaa atagaagaag atcattatgc gaaatggaat gaaggaatcg
    55261 tggaagtaac aatcgaaaat ggaaaaaatc atctctcaaa agtagaacaa acaaaactcc
    55321 caatcgttcg aggaagtatc caacatatca cgcagctatt attagggtat cgtaaagtgg
    55381 aagagttggt attccaagaa agaatacagg tgacaaaaga atatgaagaa acgttaagct
    55441 caatcttgcc acagcaacag ccaattctta atgactattt ttgataaaaa acgggtgatg
    55501 acaatatttt tgtcaccacc cttttgtttt tcatttggca tttgaaggaa aatgttaaag
    55561 taaatcctct tttttcgtaa atttgaattt tttgttttct tttgttttag ggttttcttt
    55621 tttgtggtct tggattgctt ctttcgtaat cttttcccga ttttttttag atgaatctaa
    55681 gcgttctgcg tatacttttt cttttggctt atcattcatt ggaaaggacc tccctacatt
    55741 atttcattct acctctagtt tatcatctgt taaggactag tggaaacgtt ttgcctaact
    55801 tataagctga tttttttagc agataagctt gcaagtaaaa aacaaacaag ctacaattga
    55861 tttattgaaa ttgttcctgt agctcagtag gatagagcaa tcgcctccta agcgataggt
    55921 cgctggttca attccagtca ggaacgtata aataacccct gtatcctttg cggtacaggg
    55981 gtttgttttg tatttgtgcc aaaaatgtgc caaactagtt atcattttta tataaactat
    56041 cgaacacagt attgattttt tctttatcct gatcttcaag ctctcgaaca atatgaagat
    56101 aagtagacat tgtagtttct aaactgctat gtcctaaacg tttcgatacg ctaagtatat
    56161 ttactccttg ataaagtaaa atagatgcat gtgtatgcct tagtccatgc aaagtaaatt
    56221 gcttttcaat ccctaattct tttaattttt tgcgcaaata tttgctgacg gcgtttgatg
    56281 aaactaatcc attttttaag ttgaaaaaaa caaaattatg agggtttttg atttcaaaat
    56341 tctcgaataa ttctttttga ttgattttaa acttttttaa gagatcaatc gtatgtttat
    56401 caatggaaat tttcctattt gaagtttcgt tttttgtttt tccccattca tttaatttat
    56461 aattccatgt tttattaata gtgatgattt gatcttcgaa atcgatatct tcccatgtta
    56521 atcctagaag tccagcaaat cgcattccag tggcaccagc aactaacacg agcataggag
    56581 aagagtactg cgcagaaagg ttttcttcta cgagtttcat taaagattta aattgatcat
    56641 aatctaaata tttatcttct tttggcttca atgaatcttt tcctttgatt actgcttttc
    56701 tagttggatc aaacggaatt aatccttctt ctacggcatc tttcaacgac gctctaatat
    56761 ggttattaaa cttaataacg gtagatttta catggtcttt agcatactta tttaaaaagc
    56821 gttgatatcc cactctatct aaatcagaaa tcaataccgc tggcatatat ttctttatgt
    56881 tcattaacgt atcttcgtat tttctataag tgataggaga tactgttcct tctttataaa
    56941 gttgcatcca atcctcaaag taatctgata ataatagatt tttgcgatcc cctttaaggc
    57001 ctttgttcaa gttatattct aattcgttgg cggcatcttt tgcttctcct tttgtcttaa
    57061 atcctgattt tcttatcttc gtgtattttc cgtcatcttt tttgtatgaa atttcgtatt
    57121 gccaactatt tccacgtttg actaatcttg ccataattga atcagctctc tttctctgat
    57181 acaataggaa ctataaagaa gcccattgtt tgggttttat tattttttgc acatcctttt
    57241 tgcttgccgg cgcaaggatg tgctatttgt cgttttaatg gtataattgg atgtgtatta
    57301 gatcatccaa tcagaggtgt ttattatgat taaagtgatt tttaaaagaa taactgaact
    57361 tccagagttt agattatttg ttgattattg gtatgtctgg ttttttattg ttgcattgct
    57421 gataggtcta gatgtcatat tagaaagaaa acttaagtaa gagttgagtt aataaattga
    57481 attcctaaat gagatatttt gtagcccata ttttgtttgc cgacatcacc taatactatg
    57541 gagctaatcg ttttttctaa cagaccaaaa tcagataatc tatttaagct accatctatt
    57601 agctcttgtg aatagccaga catgctgata tttccagtga aattttcgtt tccacgactc
    57661 attaagcatg ctaacaattg aatctcaatc gatgttaatt gattcagtac aagaagataa
    57721 tactcctcgt tatcccatga agcgttgtta attcgaagta aacaattctt ataaagattt
    57781 ttatacatat ctattttatt ttgtgccttt gatttttcta cctcgtcatt tatcccttcg
    57841 aatattccaa tgagttggtc cttgttctct aagttgaaaa catcttctgg aatttgatcg
    57901 cgaatgtttt ctaatgattc gtttaaattt ttgtagaatt tctcgatgcg tttgaaacgc
    57961 ttctcgtttt tagacccaaa ataaagtgac tgcacaacgc caccaatata aggaattgct
    58021 ccaataccgc cttcaactat taattcaaga tattcttttt tggttagttt ttcattgcta
    58081 ttatccatag ttacctccta ttttgtcatt tcctttacaa gcattgtgac tcctgatggc
    58141 actcttttgt ataggtttta ttattgtgca caatctaact cttagtgggg cgtggttcat
    58201 ttatttttta cttactttac tattaatcat atcccattga gcataggatt tttctatgtt
    58261 cgatcctctt gaactaattt ctcctgtaaa agttacttta tcgccctttg taaaggtatc
    58321 agcattgttt aagtccttcg caaaaattac atatgaaact ttaggtgttg cagaaatatc
    58381 attccaactt ttatcatcgt actctttgtc tgctataatg gctattctag tagtcattga
    58441 ttcgataaca gttccttcaa aagtatatgt tttaccacta atatatttag taaaagctgt
    58501 attttgctca gtagctggag taatagaata ataactatct aagaaagcac cccagtcatg
    58561 attatttgtc tctaaaaatt cttcaaattg agttaggctt ttattgctgc tgttagatgt
    58621 agtagtttga gtatcagatg atgagctttc ctgtttgtta ttgtcatttg cctcgtttga
    58681 tgaggaaagc gagaaaccta cggtagtaaa aataatcccg ataataagga gaatcatagg
    58741 tgttttctta tttgtttttt tgataaatct tacaattaac atgattaaac ctataataaa
    58801 taaaacaata ccagtaaaac caaatatacc gtccattaat agatcctctt tctcttttga
    58861 tattgaatat tataaaccaa gcaattcttt tttcttaaaa tcgaattctt cttgattaat
    58921 aatcccttca tctaacaact ctttatactt tttaatttca tctgcacttg aaatttgtgt
    58981 ttctttggaa ttattatcta atatagattg ttgcttctct atatattttt ttaaattttc
    59041 catttcattc caatattttt ttgtaaacat aacggtgttt tcatctttcg tggccgctaa
    59101 tatacctccg cgactttcgt ttccgcctag caaggtaaat tgtatataac cattactcat
    59161 acctggtttt tttaattgta cggctgatat atttttaaag gggatagttt tttctccttt
    59221 aataccttga ttaactaaat ttataaatcc ttttctaata attgaaatag aatcatcagt
    59281 tactttaatc aaggtttttc ctggagattt tattaaaatc tctttttcca ttttttcttc
    59341 ctcatttctg tgatatgatt ttttttgtag aagctcatat atgaggaaag agcccgtgtt
    59401 gcagcacggg cttttttaat gttttgctga aatcggtttt tttaataatt cttgatgtat
    59461 tcatacatat tgccttgcgt aagaatattt tttcttaaaa tagcattggc agacagcata
    59521 acaagtgtat ctgagcttat tctgaataga ataatactcc ataaattttt cgagattaaa
    59581 ctgagattca tcagtcagtt cgttctcaat atagatattt aataaaatta gaatagctat
    59641 tttatctgct tctgtttcaa attttgagtg aaaagttgta gaagtatcgt acaaaactga
    59701 aaattcaaaa tgagaggcgc tgaaatgtgc aagttcatga gataaatgaa aggcttctgc
    59761 agtttcaccg tatagatttt cattcaaaaa aatgattctg ggttttggat agtagaaacc
    59821 aggctctttc atctccatat aaactacttt caaattgtat tcgctcaaca tttctttcaa
    59881 tttcaaatac atacaaacca tcactccaac tattcatttt cctctaaagc tttagcaatt
    59941 gcaatcgctt tacgcattgt ctccttagat atttcttttc catcaaaaga aaaaacagta
    60001 tcgtcttctg ataaatccac atgtttaggg gtttctcttt cttctctacc tagaaggtag
    60061 tctacagaga catcgaaata gtcagcaatc cgacttaatt catctgaatt aggagtatta
    60121 tttttccact ttgctagata accgttagaa tatccaaaat taatttctaa ctgccgtata
    60181 gatatctttt tcctcttcgc caagtctttt attatttcat aggtattcat cgataaaaca
    60241 acctttctaa atgtttacaa aaaaagttta gaaaaatacg cagaaataat ttgactaatt
    60301 ctgagtaatg agctatacta tatcttgtaa acagatttaa caactaaaaa gacaacaaaa
    60361 aacactattg atatgtaaat gcagaccgcc aagaaagctt taaaatcaat gtttttatgt
    60421 cttatttaat tatgctctta gtatagagta ttactcagat actgtcaatt gaatttagaa
    60481 aaaagttgtt aaatttgttt acgaatataa aagaaaggaa tgagaaatat gaacacaccg
    60541 caaattttta atttcgaaca aaacgaagtt cggacaattt tagtaaatga tgaaccatat
    60601 tttgtaggca aagacgttgc gagtgttttg ggttattcaa acactaaaga tgctttgtcg
    60661 cggcatgtag atttggaaga taagatgggg tcgcgaatca cgacctcagg tcaatcaaga
    60721 gagatgacaa tcatcaacga atctggtttg tacagtttaa tcttaaaatc aaaacttccc
    60781 tctgccaaaa aatttaaacg ttgggtaaca agtgaagtgt tgccagcaat tagaaaacat
    60841 ggaggttatc taactccaga aaaagtagaa gaagctttgc ttaatccaga tacaatcatt
    60901 caattagcaa ctcaactaaa agaagaaaga actggaagat taatcgcaga acaaaagatt
    60961 gcagagtacg aacctaaaat ctcctattta gatagcatat tatcttctac agattcagta
    61021 acaattagtc agattgcagc agattatggg atgtctccac aacagatgaa taaattactt
    61081 cataaactag gtattcagaa aaaagtcggt aaccaatggt tattgtgcaa aaaacacatg
    61141 aaccaaggat acacaaaatc tcatacaact gagatcccga aagccgatgg tggcactaaa
    61201 attgtaatga ataccaaatg gacacagaaa gggcgtctat ttatctacga attactaaaa
    61261 aaagaaggat attaccctca aatggattta gaggaaattg gttagaaagg agttttagta
    61321 tgactgacat tgcagaaatc actcaacgag atagagaaaa aatcaaagaa tatgtcgaaa
    61381 gttcaaagtt cttaacttac accatgcttg ctgaaagatt tggaattagc aaaagctact
    61441 tatctttaat tttaaacggt aaaaagactt ctgcagaagc aaacagaatt atagattcga
    61501 ttatcactat gtacgaattg taaagggagg aacagctaat gcaatatcta gaagcaaaaa
    61561 tcccaattcc agaaggctat gtaattatct cccaagtgga ttatgaggag ttaaaaaaag
    61621 ctgatgatac tggtagatgg atgacgttgc cagaagtgct agaacggatt aacagaaaat
    61681 atgattggtt tacttctaga gttttaaaga acccaagata tagaaatatt atcgatatcg
    61741 agaaaaacaa aaatggattt gtctattatc cagttgaagg aagagacaca tatctatttt
    61801 taagaagtaa aacacttgaa tttttagaaa caaatttttc ggaaatctta aggaggcaag
    61861 cggatgggaa aatttaatag agcgctagtg ttcagcgcac cgctaatcat ctacgcttta
    61921 ggactttggg gaagcaggca agcgttgata ggaacgattg tttacatggt ttggattttt
    61981 atggggcttg atgaagctga agctgagtac agagcgaaaa agccaaccga gggggctgac
    62041 taaaaatgaa tagaaaagag aaactagaat gtatattact attactcagt ttaattcttt
    62101 cactaatttc tctattggga agtttgtatt tttgatatca acaattttgt ggatttgggt
    62161 tctatttaga gtaaatatca tttttgtttg gtgattaagg agtttattaa aattaatttt
    62221 ccctacttca aatgcaaggt aacaagatat agcactttta ggaggaatat ttagcggtac
    62281 agcttcagag tatattctat tatcatcgac tgatcctcct ttagcaatta atttcttaaa
    62341 tggagtagct gaccatctgc ggtttaagtt agcggatgaa agttctaaat taactaacat
    62401 agctggttct gttgaatagt tagaaatgat tacttttgta taaatcatat ctgcagcgaa
    62461 gtaagaagca ttaagttcaa cttctagttg agggcgcatt ccttttattt ttatccctgt
    62521 taaaaacgta ctcaatataa aacttattat tgacatccat tgaaaaattg ttaagtgaaa
    62581 aaaattcaaa ataaacacca ccagttttta actaaattat accaaaaagg agagaagaaa
    62641 taatgcaaga attagtaatt ttgaaaaata aagaagctgt gactacgagc ttacaagtgg
    62701 cagaaagctt cgagaaaaaa catcaacatg ttttaagaga tattgatgca ctaaaaaaag
    62761 atgtgtccaa ttttggacag atgtttgtag aaggtaatga accagattca tatggcagaa
    62821 atcgacgagt tttcttcatt agtagagatg gatttttctt gctggctatg gggtttacag
    62881 gaaagaaagc tatctacttc aaacaaaaat acattgaagc attcaacgaa atggaagatg
    62941 ttattcgcaa gaatactgtt cctcaaacaa ttgaagatat gatgatctat caattagaag
    63001 aaatgaaaga tgttaaaaaa gatgtttcca tgcttaaaga tactatgcga attagcggac
    63061 aacaagagtt tgaaattaag caaaaaggaa atatgaaagt tatgggagtt ctagggggaa
    63121 aagaaagccg agcttatgaa gaaatcagca aaaaagtatt ctcaaaattt tggtctgaat
    63181 ttaaacgtac cttttcaatc ccaagatatg gcgagttacc tcgtaagaga ttcgatgatg
    63241 ctgtttcatt tattgaaatg tggttgccag aaactgcgat ccgtatggaa attgatcaac
    63301 tgaacagaca acagagactt tttggtgatg acaatgaata gagctgaagc gctaagaata
    63361 gggacggcaa ttgctaattg ctggtggaaa tactataaac caatcatcct aagccaacaa
    63421 catattgaca agcaaaaagc atggcaacaa ataaaaaagt gactccgccg gcaagcaaag
    63481 agtcacaaag aaaacacatc ataaggagat tttagcatat ggaaaaagaa ctttccactc
    63541 tagatcaata tttgattgat cctgattggg gcaagccgaa aattgaggaa acaagtggtc
    63601 gaaaaatcag acgaaatctt ttgacgaatg aagaactagc ttgggatcaa gatgatttag
    63661 gcaaccatgt aactatttgg gatcatgttt atcttatcca tctatcgaag cattcgaata
    63721 aacctgaata tatttacgtc atcgaagatg gcttgattga tgcgctagaa gagtacgaca
    63781 gagataactt gattgatatc tcttattacg gaccaggtaa gaaatacatt gctgaaatgg
    63841 aggcagaatt tgatgagtga aatcaaaggg acaacgaact ttgaaaaact ttttagtcgt
    63901 aagttaaata aaattctcaa gaaaaaagga aattttgatt atttatcttg ggctcacgcg
    63961 tgggagatta tgaaaaagaa tgatccacag gcaacggtaa ctattaatga gtacaaacac
    64021 tacagggttg tttctggaac tcatcaagac tttcttgttg aggaatataa accttttctt
    64081 atggatgaaa ctgggactta tgtatctgtc tcagtaacgg ttaaaggaca cacggaaacc
    64141 gaattatttc ctgttttaga ttatcgaaac caaccagttg ttaaaccaaa tgcaatgcaa
    64201 atcaataact cattgaagcg atgctttgtg aaagcattgg ctctacacgg actgggatta
    64261 tatgtatttc aaggggaaga tattccaaca ccacctagaa tcgatacaaa gaaattaaac
    64321 atgctagaga cgattctaga agctttcaat gagcagatgg gtaaagatat gaccaaaacc
    64381 ttaatcgaat atgttaatga gcagacagat aaattagggc tcttagctga taacgttgaa
    64441 actattgaac agttaagcta tgagcaatgt gccttgatgg agcgagcaat agcagctaag
    64501 agaaaagaat tagataagaa gtgatatgag tgtttaaacc attaatcgat tcatattcag
    64561 cggttctgaa aaagttcaaa ggaaaagaca taagcgcaac catcaatgag gaagtgaaca
    64621 ttgatcgact aaagacgatg tatgacggct acgatggtga tcgagtcatt gaaattcgtt
    64681 ttattgatcc tagacgtttc accgtacagc aacgaaactt catctatgcg ctgataggcg
    64741 atatttttat cgatacaggc atgccaacgg acttctggaa ggaattcttc tactttcgtt
    64801 ttgaaggtgt cacagggcgc aaaataagcc tgaaagacga atcgaataca actgtgagtg
    64861 atgccaatgt cttagcaaat atcatcttag atttcatctt tgaacatcat attcctttca
    64921 aagaaggcta tgagatttta cctgcgaatc aagaatatta cttctacaaa tgcatcacaa
    64981 aaagagtctg ctgcatctgt ggcaaaacag gagctgacat cgatcacttt gacaaagcgc
    65041 taggaagacg aaagcgcaaa gaagttgatc atgcagagta cacatttgca gcactctgca
    65101 gaatccatca cacagagaag cacaaaatag gtgtgattaa tttcaaaaat aaatatcaaa
    65161 tcaaagggat caaattaaac cagaaaacaa tcaaaaagtt aaatattgga ggggaaaaat
    65221 gacagaacat cgaagttatt atgcgattat accagccaac gtaaggtacg acaaaagact
    65281 taaaccaaat actaagttgt tatacggaga gataacggcc ttgtgtaatg aaagaggctt
    65341 ttgttgggca ggcaatgagt actttgcaga tttatatggt gtgaataaag agaccatatc
    65401 gcgatgggta agtgatttga ttaagtttgg atacttgaat cgggaaatca tttacaaaga
    65461 gggtaccaat caaataatca ataggtacct acgaattaat caatacccta ttgacgaaaa
    65521 acgcaatacc cctattgacg aaaaagtcaa agataataat acatctatta ataatacatt
    65581 taataataca aaagaatata taagagagtt accgccttcg aaaaaatcga aggctaagcc
    65641 cgtccgtcat aaatacggag agtataaaaa tgttcttttg tcagatgagc agatggagaa
    65701 actcaaaaca gaattcccta atgattacca agagcgaatc gaacgactgt cagagtattg
    65761 tgaatcatct ggtaagactt ataaaaacta tttggcaact attcgaagtt gggcaaggaa
    65821 agaaaaaagt gaacctaaga acgcaagcag tggatacaag cgcacaggaa gacgagagaa
    65881 gcttcctgaa tgggcaatcg accaagaagc ctatctcaag aaaaaagcgc tagaacgagc
    65941 taatagacaa tcaaaagcac cattctaaga ggtggaaaaa tgaagatcga ttatctagaa
    66001 ctaattaatg aaatagcgaa ttataaaaag ggcgaggaat tagacgtcct gagagacgta
    66061 tatgatcaac tcgaagaagc tggaattgaa ggaattaaga atgatcgttc gagttggagt
    66121 aaactcagat actatttcgc actctatatc gatggaacgc aattaagaaa tttagcatat
    66181 accaaattac tattcattga ttgtgttaaa ggcttgcaaa aacatcttaa tgaacttgag
    66241 caggtgtaaa caagatggac ctaaagacat ttacagcaca gatcgaatta atgcatcaag
    66301 aagctttaag acaaagtgtg tcgtacgaag acaagtggct caacacattt catggcggac
    66361 gtgagagcgc acttgatcaa gtactcaaat tactgaaagg agaatgtcgg gatggataag
    66421 aaagcggcaa tgcagcgaat tatcgaattg acttattcag aagattggca ggaagataaa
    66481 aaaatagttg cagaagtcca aaagctcggt aaatcaatgt ggactgaaaa gcccaaacgg
    66541 agaacgccga gaaagattgc aatctggcat ggtgatcgaa ttctagtaac gggtaccgct
    66601 aaacagttat ctgaaattac tggtctgagc aaaaatatta tttgggatag agctaggagc
    66661 ttatggattg attcaaaagg aagacagttt aggtatgtgg aggagaaata atggacgaac
    66721 taatcgcgaa agtagagcag tgggcaaaag ataagggatt ggatcaagcg gatttcagca
    66781 agcaaatgtt aaaaacggtc gaagaaatcg gggaagttgc cgcttctcta gctagaaaag
    66841 atgaacatgg tttaagagac ggaattggcg atgtagtagt aaccttgatc atcttggcaa
    66901 tgcaaaatga tatggattta tacgagtgct tggattttgc ttatgacgaa atcaaaggtc
    66961 ggactggaaa gatggtcaat ggtgttttcg ttaagtccag tgacctgaaa taattccatt
    67021 aatggaagaa ataactgaat aggaggatag acatgattga tgcaaaacaa gtgcaaaaac
    67081 agaaagatgg aatgttgatg tttgaagcat atgttctacc atttcttaat caatttgaag
    67141 ttttggaatg ctctgccagc ggagaagaat tggagtatgt tgtaattcgt gaaaccaaag
    67201 aaaacgttca gaagctaaac gaatttcttt gcactattaa ctgttgggat atgatagctc
    67261 caggattttt atgtccagct atgggagaat ttttggaata ttgtcgtctt gaagatgcag
    67321 gcactttgga tttagcttat ctggtataca actacctaaa catcaacaca gatcatttgt
    67381 ggtttggcac tgccgaaaga aaatgggtag tccgctaacc accaatatca atgaaaaaag
    67441 gcggtttcct gaaagtggaa ttgccgccat tgctgtaagg ttctttacgc gactcaaggt
    67501 cacagaaata taaatgcaaa tctcttttga agtgatccaa agctctcgga aaatggaagt
    67561 tttttgtttc aaagaataaa tgagatttgc gtcaagaacc tgagagttat ttccaaaaaa
    67621 gcttccaaag aaccgaatga gctaaggaca tcatgtgtaa acacataata tagctctcct
    67681 ttcacggcta atcataacat gaaaaatatt cgatgtcagt aggaaaaaag gtgaattatg
    67741 aaaagaatat taaattatcc cggcagtaac agattctgca attaaactcc gaagctgatg
    67801 tatcagtatc aactgtagaa tattatgagc gtggagaaag tcaagcagtc acaaatgtaa
    67861 aaatcgataa cgtcaacggt cttatctgtt tagatgaagg cgatagtatg aatgaaattg
    67921 ctgcagacca tgaattatca gaaactgctt taattgatca aatcgaatta gctagacaat
    67981 actttgttcc atatgcagat acctggtcca agcataagaa tgacatcatt tttccagagg
    68041 aacaatgaga gcaactgatc cagtaattat ccttgaggaa gccaaattta tttggactca
    68101 cgaagagata gagcaagcac gcttgctctt ttctcaagga gttaagccga gcaaagtagc
    68161 tgaaataatg ggtcaaaaga ttcttgatgt cggattgctt ttgctccatc tagcagaaaa
    68221 aatttgattt gaggtgaaaa tgatgattca acttgcaggc atccaaacag ggaaaattta
    68281 tttttctgga gaaagcaaaa gcgaagccaa ccaatggcta ttgaaaactt acacgaacaa
    68341 taagaagcta aggaaaaaat atcctgattt gttgctaaag gatgatcaga ttatgccgga
    68401 accgatgatt ttgacccgta aggagggaag ttgaaactaa aaaaccagcc gtgtgggctg
    68461 gagttgctat ttctttttaa taatgagttt ttctaaattt aattcattct cacgagtttg
    68521 cgtccaataa tcagaataat attttaattg tttaagagta ggaatgtcgt cttcggtaaa
    68581 attagaaaat aattctggag aattatcttt ttttaattgt tctaatcctg attctttaaa
    68641 acgttgttca acgagtttta ctaaattgtt gatattcatg ataaatcacc tttttaaatt
    68701 taatttcgat ttgattaatc gatacaagta ttatatcaaa taaaaaagct gatttagggg
    68761 gtcttacaaa aacactaaaa ttttttcaaa aagaggagga acggtggtga aagtcagaga
    68821 acaattcaag atagtgagcg acgaaggtaa tgtttacaaa ataaagatcg ttaatattaa
    68881 tgaatatcga gaagcagatc acaaatattt ggttgactta tacatgaacg ataaatattt
    68941 gggagaagga ttttgtggag attcactttt tgatgatgag cgaattttga aggaggaaac
    69001 agaatgatcc acgaactaaa aatactacca gaatattttg aagcagtgac gagcggacgc
    69061 aaacaattcg agatccgcaa gaatgatcgt gacttcaaag ttggtgatca gttaatacta
    69121 cgtgaatata aaaaatacat taaacatgaa ttacctggac taacgagacc aacatttagt
    69181 tacactggtg actcatacaa agcagagatc acttatatta ctgattacat gcagaagtcc
    69241 ggatatgtag tgttggggat taaaggggtg aattaaatga aagtatacat cttagctatc
    69301 acagagggca catggatgtt cccagtcggt tccggaaaaa tctataaatc aaaaacagct
    69361 gcttataaag catttgaaaa atacaaaaaa gaaaacggtg gcggcactaa cgcaaagatt
    69421 ctagtggctg ataactggca tgaagaaggt gaacgtaatt gatttacctg ttcattttcg
    69481 tggtactaac tatttttatg ctagtaatct tattcaaaaa aagatgagta aagacgaacg
    69541 aaaaattctg gccaactttt atttagtatt aaaaatcgaa caaaatgtgt tttcggaaag
    69601 gagaattgta tgggaaaaac aaaatcaaag attaagaaga aaaaacgacg tttggaacaa
    69661 aaggcaatcc agaatggaac ggctaagaaa aaataaaaaa gctacccctt tcgagatagc
    69721 agcgcaatac tattctacca taaggggtgg cgtttgtgag atttcattgg ttaaaagatt
    69781 accaagaact agatgagcag attctttact taaagtggaa tcttaataaa agtaagcttg
    69841 aattgaatcg atgggtctgt ggtgatttag caaacgtccg catcgaaaag aattcgagaa
    69901 cttcttcttt agaagagaat atccaaaaga tagaaaatga attggaattg ttgatcgagc
    69961 aaaaagaaga aatgttattg ctgatagata gcttttctgg tatcgataat caaattgtta
    70021 agatgaaata tgttgatcaa atgagcttag aagatattgc tgaatctgtt gggtatagct
    70081 catcgtatgt cagacagcga catgcagaaa ttagaaagac attgaacttt ttagatgagt
    70141 atgaacacag acaagctgac cgattgaaaa aagaaaacga aattgatttt tacaatagcg
    70201 agaagtacaa agaacaactc tctttattct aaaattacac tgttcactaa atgttcggtc
    70261 attgtagcta tgtaaacatt gttagcagca tgatattcta ttagtgtcaa aaaaatatga
    70321 aagagccaag atatcccaac cgttttatta attggtatct gtggctcttt tattctttta
    70381 attagtagtt ggcataaagt tatttccatt tataaattga tcagctaaat cagcatcata
    70441 atcagccgcg ataaattgcg ctgctgattt aataaatgca tccatatcat gaacatcttt
    70501 attagtatgt ctacgaacat agtgcgtttc atcattacca atccaagtag cagctttggc
    70561 taatctttgg agtttaggaa aatcattcaa ataattttca attgttttac ctaataacat
    70621 tttttttatt ttatcttcat cctctgaatt tttagatatt gcataatctt taattagaaa
    70681 ttctagagct tttctataac caactccagc aatttgttcc aattgctcag atttagcttg
    70741 tgtg
//
LOCUS       DAZKNH010000003        62289 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000003 DAZKNH010000000
VERSION     DAZKNH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.003"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            472..1335
                     /locus_tag="ACG8MG_000164"
     CDS             472..1335
                     /locus_tag="ACG8MG_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0374243.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1464..3086)
                     /locus_tag="ACG8MG_000165"
     CDS             complement(1464..3086)
                     /locus_tag="ACG8MG_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0374244.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3335..3856
                     /locus_tag="ACG8MG_000166"
     CDS             3335..3856
                     /locus_tag="ACG8MG_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0374245.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4042..5772)
                     /gene="spxB"
                     /locus_tag="ACG8MG_000167"
     CDS             complement(4042..5772)
                     /gene="spxB"
                     /locus_tag="ACG8MG_000167"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFQ0374246.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5937..6755
                     /locus_tag="ACG8MG_000168"
     CDS             5937..6755
                     /locus_tag="ACG8MG_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFQ0374247.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6858..7055)
                     /locus_tag="ACG8MG_000169"
     CDS             complement(6858..7055)
                     /locus_tag="ACG8MG_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374248.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7224..7802
                     /locus_tag="ACG8MG_000170"
     CDS             7224..7802
                     /locus_tag="ACG8MG_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0374249.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7897..8034
                     /locus_tag="ACG8MG_000171"
     CDS             7897..8034
                     /locus_tag="ACG8MG_000171"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374250.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8847..9350)
                     /locus_tag="ACG8MG_000172"
     CDS             complement(8847..9350)
                     /locus_tag="ACG8MG_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0374251.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9709..10734
                     /locus_tag="ACG8MG_000173"
     CDS             9709..10734
                     /locus_tag="ACG8MG_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0374252.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11057..11935
                     /locus_tag="ACG8MG_000174"
     CDS             11057..11935
                     /locus_tag="ACG8MG_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFQ0374253.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12411..12527
                     /locus_tag="ACG8MG_000175"
     CDS             12411..12527
                     /locus_tag="ACG8MG_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0374254.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13455..13793
                     /locus_tag="ACG8MG_000176"
     CDS             13455..13793
                     /locus_tag="ACG8MG_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374255.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13887..14117
                     /locus_tag="ACG8MG_000177"
     CDS             13887..14117
                     /locus_tag="ACG8MG_000177"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFQ0374256.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14517..14888
                     /locus_tag="ACG8MG_000178"
     CDS             14517..14888
                     /locus_tag="ACG8MG_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFQ0374257.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15162..15854)
                     /locus_tag="ACG8MG_000179"
     CDS             complement(15162..15854)
                     /locus_tag="ACG8MG_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0374258.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15857..16642)
                     /locus_tag="ACG8MG_000180"
     CDS             complement(15857..16642)
                     /locus_tag="ACG8MG_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0374259.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16879..18279
                     /gene="lacG"
                     /locus_tag="ACG8MG_000181"
     CDS             16879..18279
                     /gene="lacG"
                     /locus_tag="ACG8MG_000181"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFQ0374260.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18304..18621
                     /locus_tag="ACG8MG_000182"
     CDS             18304..18621
                     /locus_tag="ACG8MG_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0374261.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18634..20307
                     /locus_tag="ACG8MG_000183"
     CDS             18634..20307
                     /locus_tag="ACG8MG_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFQ0374262.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20321..21952
                     /locus_tag="ACG8MG_000184"
     CDS             20321..21952
                     /locus_tag="ACG8MG_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFQ0374263.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22443..24008)
                     /gene="guaA"
                     /locus_tag="ACG8MG_000185"
     CDS             complement(22443..24008)
                     /gene="guaA"
                     /locus_tag="ACG8MG_000185"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0374264.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(24067..24990)
                     /gene="coaA"
                     /locus_tag="ACG8MG_000186"
     CDS             complement(24067..24990)
                     /gene="coaA"
                     /locus_tag="ACG8MG_000186"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFQ0374265.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            25186..25791
                     /locus_tag="ACG8MG_000187"
     CDS             25186..25791
                     /locus_tag="ACG8MG_000187"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0374266.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            26099..27115
                     /locus_tag="ACG8MG_000188"
     CDS             26099..27115
                     /locus_tag="ACG8MG_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0374267.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            27256..28557
                     /locus_tag="ACG8MG_000189"
     CDS             27256..28557
                     /locus_tag="ACG8MG_000189"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFQ0374268.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28603..29265
                     /gene="deoC"
                     /locus_tag="ACG8MG_000190"
     CDS             28603..29265
                     /gene="deoC"
                     /locus_tag="ACG8MG_000190"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFQ0374269.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            29286..29684
                     /locus_tag="ACG8MG_000191"
     CDS             29286..29684
                     /locus_tag="ACG8MG_000191"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFQ0374270.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29750..30832
                     /locus_tag="ACG8MG_000192"
     CDS             29750..30832
                     /locus_tag="ACG8MG_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0374271.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            31044..32606
                     /locus_tag="ACG8MG_000193"
     CDS             31044..32606
                     /locus_tag="ACG8MG_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0374272.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32599..33732
                     /locus_tag="ACG8MG_000194"
     CDS             32599..33732
                     /locus_tag="ACG8MG_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0374273.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33732..34688
                     /locus_tag="ACG8MG_000195"
     CDS             33732..34688
                     /locus_tag="ACG8MG_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0374274.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34787..35953
                     /gene="deoB"
                     /locus_tag="ACG8MG_000196"
     CDS             34787..35953
                     /gene="deoB"
                     /locus_tag="ACG8MG_000196"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFQ0374275.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35977..36795
                     /locus_tag="ACG8MG_000197"
     CDS             35977..36795
                     /locus_tag="ACG8MG_000197"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0374276.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36812..37519
                     /gene="deoD"
                     /locus_tag="ACG8MG_000198"
     CDS             36812..37519
                     /gene="deoD"
                     /locus_tag="ACG8MG_000198"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0374277.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(37817..38503)
                     /gene="gpmA"
                     /locus_tag="ACG8MG_000199"
     CDS             complement(37817..38503)
                     /gene="gpmA"
                     /locus_tag="ACG8MG_000199"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0374278.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(38675..39256)
                     /locus_tag="ACG8MG_000200"
     CDS             complement(38675..39256)
                     /locus_tag="ACG8MG_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0374279.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(39389..40072)
                     /gene="rpiA"
                     /locus_tag="ACG8MG_000201"
     CDS             complement(39389..40072)
                     /gene="rpiA"
                     /locus_tag="ACG8MG_000201"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFQ0374280.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            40391..40804
                     /gene="rpsL"
                     /locus_tag="ACG8MG_000202"
     CDS             40391..40804
                     /gene="rpsL"
                     /locus_tag="ACG8MG_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFQ0374281.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            40888..41358
                     /gene="rpsG"
                     /locus_tag="ACG8MG_000203"
     CDS             40888..41358
                     /gene="rpsG"
                     /locus_tag="ACG8MG_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFQ0374282.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            41452..43539
                     /gene="fusA"
                     /locus_tag="ACG8MG_000204"
     CDS             41452..43539
                     /gene="fusA"
                     /locus_tag="ACG8MG_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFQ0374283.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            43711..44898
                     /gene="tuf"
                     /locus_tag="ACG8MG_000205"
     CDS             43711..44898
                     /gene="tuf"
                     /locus_tag="ACG8MG_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0374284.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            45305..45613
                     /gene="rpsJ"
                     /locus_tag="ACG8MG_000206"
     CDS             45305..45613
                     /gene="rpsJ"
                     /locus_tag="ACG8MG_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFQ0374285.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            45644..46273
                     /gene="rplC"
                     /locus_tag="ACG8MG_000207"
     CDS             45644..46273
                     /gene="rplC"
                     /locus_tag="ACG8MG_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFQ0374286.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            46301..46924
                     /gene="rplD"
                     /locus_tag="ACG8MG_000208"
     CDS             46301..46924
                     /gene="rplD"
                     /locus_tag="ACG8MG_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFQ0374287.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            46924..47214
                     /gene="rplW"
                     /locus_tag="ACG8MG_000209"
     CDS             46924..47214
                     /gene="rplW"
                     /locus_tag="ACG8MG_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFQ0374288.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            47253..48086
                     /gene="rplB"
                     /locus_tag="ACG8MG_000210"
     CDS             47253..48086
                     /gene="rplB"
                     /locus_tag="ACG8MG_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFQ0374289.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            48129..48407
                     /gene="rpsS"
                     /locus_tag="ACG8MG_000211"
     CDS             48129..48407
                     /gene="rpsS"
                     /locus_tag="ACG8MG_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFQ0374290.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            48430..48777
                     /gene="rplV"
                     /locus_tag="ACG8MG_000212"
     CDS             48430..48777
                     /gene="rplV"
                     /locus_tag="ACG8MG_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFQ0374291.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            48791..49447
                     /gene="rpsC"
                     /locus_tag="ACG8MG_000213"
     CDS             48791..49447
                     /gene="rpsC"
                     /locus_tag="ACG8MG_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFQ0374292.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            49450..49884
                     /gene="rplP"
                     /locus_tag="ACG8MG_000214"
     CDS             49450..49884
                     /gene="rplP"
                     /locus_tag="ACG8MG_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFQ0374293.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            49874..50062
                     /gene="rpmC"
                     /locus_tag="ACG8MG_000215"
     CDS             49874..50062
                     /gene="rpmC"
                     /locus_tag="ACG8MG_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFQ0374294.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            50087..50353
                     /gene="rpsQ"
                     /locus_tag="ACG8MG_000216"
     CDS             50087..50353
                     /gene="rpsQ"
                     /locus_tag="ACG8MG_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFQ0374295.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            50411..50779
                     /gene="rplN"
                     /locus_tag="ACG8MG_000217"
     CDS             50411..50779
                     /gene="rplN"
                     /locus_tag="ACG8MG_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFQ0374296.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            50816..51124
                     /gene="rplX"
                     /locus_tag="ACG8MG_000218"
     CDS             50816..51124
                     /gene="rplX"
                     /locus_tag="ACG8MG_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFQ0374297.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            51152..51691
                     /gene="rplE"
                     /locus_tag="ACG8MG_000219"
     CDS             51152..51691
                     /gene="rplE"
                     /locus_tag="ACG8MG_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFQ0374298.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            51710..51895
                     /locus_tag="ACG8MG_000220"
     CDS             51710..51895
                     /locus_tag="ACG8MG_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFQ0374299.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            51931..52329
                     /gene="rpsH"
                     /locus_tag="ACG8MG_000221"
     CDS             51931..52329
                     /gene="rpsH"
                     /locus_tag="ACG8MG_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFQ0374300.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            52361..52897
                     /gene="rplF"
                     /locus_tag="ACG8MG_000222"
     CDS             52361..52897
                     /gene="rplF"
                     /locus_tag="ACG8MG_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFQ0374301.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            53058..53414
                     /gene="rplR"
                     /locus_tag="ACG8MG_000223"
     CDS             53058..53414
                     /gene="rplR"
                     /locus_tag="ACG8MG_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFQ0374302.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            53435..53935
                     /gene="rpsE"
                     /locus_tag="ACG8MG_000224"
     CDS             53435..53935
                     /gene="rpsE"
                     /locus_tag="ACG8MG_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFQ0374303.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            53950..54129
                     /gene="rpmD"
                     /locus_tag="ACG8MG_000225"
     CDS             53950..54129
                     /gene="rpmD"
                     /locus_tag="ACG8MG_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFQ0374304.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            54173..54613
                     /gene="rplO"
                     /locus_tag="ACG8MG_000226"
     CDS             54173..54613
                     /gene="rplO"
                     /locus_tag="ACG8MG_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFQ0374305.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            54613..55908
                     /gene="secY"
                     /locus_tag="ACG8MG_000227"
     CDS             54613..55908
                     /gene="secY"
                     /locus_tag="ACG8MG_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFQ0374306.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            55970..56617
                     /locus_tag="ACG8MG_000228"
     CDS             55970..56617
                     /locus_tag="ACG8MG_000228"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFQ0374307.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            56808..57026
                     /gene="infA"
                     /locus_tag="ACG8MG_000229"
     CDS             56808..57026
                     /gene="infA"
                     /locus_tag="ACG8MG_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFQ0374308.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            57059..57175
                     /gene="rpmJ"
                     /locus_tag="ACG8MG_000230"
     CDS             57059..57175
                     /gene="rpmJ"
                     /locus_tag="ACG8MG_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFQ0374309.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            57193..57558
                     /gene="rpsM"
                     /locus_tag="ACG8MG_000231"
     CDS             57193..57558
                     /gene="rpsM"
                     /locus_tag="ACG8MG_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFQ0374310.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            57586..57975
                     /gene="rpsK"
                     /locus_tag="ACG8MG_000232"
     CDS             57586..57975
                     /gene="rpsK"
                     /locus_tag="ACG8MG_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFQ0374311.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            58055..58993
                     /locus_tag="ACG8MG_000233"
     CDS             58055..58993
                     /locus_tag="ACG8MG_000233"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFQ0374312.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            59021..59401
                     /gene="rplQ"
                     /locus_tag="ACG8MG_000234"
     CDS             59021..59401
                     /gene="rplQ"
                     /locus_tag="ACG8MG_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFQ0374313.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            59673..60512
                     /locus_tag="ACG8MG_000235"
     CDS             59673..60512
                     /locus_tag="ACG8MG_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0374314.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            60488..61357
                     /locus_tag="ACG8MG_000236"
     CDS             60488..61357
                     /locus_tag="ACG8MG_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0374315.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            61347..62144
                     /locus_tag="ACG8MG_000237"
     CDS             61347..62144
                     /locus_tag="ACG8MG_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0374316.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT        19715 a        11154 c        13248 g        18172 t
ORIGIN      
        1 taaacggtgt tctcctgcca actcctcggt cacaagtcac aatattcaac aaacatgaga
       61 agctgttttt tgaaattgtt ccttgtgact cggagctaaa cggcaggctc acaacctctt
      121 ggtaaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc aaaatatgag
      181 gaactatttt ttccgttttt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc
      241 tgattcacgg tgttccagaa gtagcttcta cggaaataag ccgaaatttc acaaaaattt
      301 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
      361 tcttattttg attagaactt gcgaaaataa accggttaaa gtgtgtctag cgcaagagat
      421 ttgctataat agacgataag ggtaaaatgt aacgagaagg ggttgaaaaa tgtgtatcaa
      481 attgtaacag attcttgttg tgatctgcca taccatgtat tagcaaaaga aaaagttgat
      541 tttttatcaa tgaatctcca attgaatgat caagagttag tagatgacct cggaaagaca
      601 ttcaattatg accgctacat gaatcagctg aaaaatggtg cgatgcctac gacatcacaa
      661 atcaatgttg gccgatattt agaatttttc cgttcttatg cggaaaaaca gattcctgtt
      721 ttatatgtgg ctttttccag tggattaagc ggctcttaca gtagtgcgct tcaagcggtt
      781 gaattgctga aggaagaata tgaagaccct gagatctatg tttttgatac aaaagctgca
      841 agtattgggg aagggctttt agtattggaa gctgtaagat tgaaaaaaga aggtaaaagc
      901 atcgaagaaa ccctagagtg gctggaaagt aattatttga aagtccattc atgggtaaca
      961 gtagacgatc tgaaatatct agaacgaggc ggtaggattt ccaaagcaag tgctactctt
     1021 ggcagtctat tgagtgtaaa gcctattatt acggtggata aagaaggaag gctgacgaat
     1081 gttgacaaag ttcgaggaag aaacaagtcg atccagacaa tcgtcaatga gacggtaaaa
     1141 acaatcgttg caccacttga tcaaatcgtt tatgtggctt acgctgggga tctagagagc
     1201 gcggagaagg caaaacggct tttggaagag cagataaaga tgaaagatgt gaagctttat
     1261 cccttaggac caacgatcgc cagtcacaca ggctatggct gtatcgcgat tttttcgatg
     1321 ggaatcagtc gataaaagca gtaactcatt ctgtaaaaaa gcccagaaat tgattgtaac
     1381 aaaaacagcg actcttaaaa gatagatgag catcctatct cttagaggtc gctgtttttt
     1441 tattttttaa gctaaatagg ccattatgcc cataactcgt tcaattgttt ttgaatatct
     1501 gcattttcca aaaattcatc atatgtcgta tcagctcgat cgatcacacc tttatccgaa
     1561 acggcaatga tgcggtttgc caatgtttgg ataaattgat ggtcatgtga cgcaaataag
     1621 attgagccag taaatgccat caagccgtca ttcaatgctg tgattgattc taagtctaaa
     1681 tggttcgttg gatcatctaa tacaagtacg ttagctttcg aaagcatcat ttttgaaagc
     1741 atgcaacgga ctttctctcc accagataaa acattcactg gtttcaatac ttcttcacca
     1801 gaaaacaaca ttcttcctaa gaagcttctt aagaaggtgt tatcatcttc ttctttactt
     1861 gcatattgac gcaaccagtc taaaatcgtt agatcagtat cgaattcttt cgttgtatcc
     1921 tttggtaaat aagaattact tgttgtcaca ccccaacgta ctgttccagt atctggcgta
     1981 atttcaccca tgataacttt gaatagcgtt gttgttgtta tatcattttc tgcgacgaaa
     2041 gcgaccttgt cttctttgct caaattaaaa gatatatcat ctaaaatttt ctttccatca
     2101 atcgttacac ttacattttc tacctgcaat aagtcgtttc caatctcacg ttcaggtttg
     2161 aatccaacga atggataacg tctagaagaa ggttggatat cgtctaaagt aattttttca
     2221 agcatttttt tacgtgaagt tgcttgtttt gattttgatg cattcgcact aaagcgggca
     2281 ataaattctt ggagttcttt gatttgttct tcttttttgg catttgcgtt tgcttgaagt
     2341 ttagccgcta gttgactaga ttccaaccaa aaatcataat ttcctacata aagtttgatt
     2401 ttaccaaaat ctagatccgc catatgtgta catactttgt tcaaaaagtg acggtcatgg
     2461 gatacaacga tcacagtatt ttcaaaattg atcaaaaatt cttccagcca attgatcgat
     2521 tggatatcca gaccgtttgt tggttcgtct agcaaaagaa catcaggttt accaaatagt
     2581 gtttgtgcta ataacacttt taccttttgt ccaccagtta attcactcat ttgctgatgg
     2641 tgcaattctt cagggatgtt cagaccttgc agtaaaacag ctgcttctgg ttcagcttcc
     2701 catccattaa gctcagcaaa ttctccttct aattcagctg ctcgaatacc atcttcgtca
     2761 gtgaaatcct ctttcatgta aatcgcatct ttttctttca gcacttcata cagacgcttg
     2821 tgtcccatga tgactgtttc aagtacagta tagtcttcat agtcgtaatg gttctgtttc
     2881 aatacggcca tccgttcatc tggtcccata gtaacggaac ctgtggaagg ctgcagatcc
     2941 cctgataaca ctttaaggaa cgttgattta cctgcaccat ttgcaccaat caagccatag
     3001 cagtttccgg gggtaaattt aatattcaca tcatcaaata gtttgcggtc tgaaaagtgc
     3061 aaactcacat catttacagt aatcaatttt tttcctcact tttatcatta ttagttttga
     3121 caagtcttat agaattatat aggggaaagg taaaagattc aaggcaatcg aacattcttt
     3181 gtgtttcacg tgaaacagaa tgaaaaaaat aaaaataaaa gcggagcgaa aatagctact
     3241 ataggattta ttagtttgca acagcataat ttttgacgtt atttataaga gggagtacta
     3301 taaaactgta aaagaaatga aagaaggatt gaaaatggat gaaaaaagaa ccatcgactg
     3361 gggctcattg attttgggaa ttttatttgt acttgtctca ttgttatcat ttcaagaccc
     3421 tgtcggaaat ttagttgcaa ttgtagtagt ctttgcgatt tttgccttta ttaaagggat
     3481 atttgaactg tttgtacgga acagattaaa agagctaaca ggatataaag gaaagatgcc
     3541 cttaataatc gggatcatcg atattctagt tggcgtcttt ttcttattca atattggtgc
     3601 aggagtcgca gcattgcctt ttgtttttgc agtatggttt attgctgatt cagttttagc
     3661 gttatttact gctgacttgg caagaggagt aagtgaagga tattattggt ttacgatcat
     3721 cgtcaatatt ttaggaattt tattaggaat tttcttattg tttaatccaa tttcttctgc
     3781 gttgacatta agctttttag ttggattcta ttttatgtta tttggtatca cacatatcgt
     3841 ctatgcattt agataagtaa aatcaaacaa ataaaaaagc cggcttccga ttacagtttt
     3901 actgttgtaa ttggaagccg gcttttttat catggacaaa tatgcacgag gtgattttta
     3961 ggtgtcgaga caaatttatg caaaaatgtg agaagctata tacacaaatt ttatcttaat
     4021 actcgaagca tagcgcagtt gtcacaacct cttttcatat aattcattca atactggcaa
     4081 atcatggact tcatattttt ctttgaatgc ttggatttct tcttcagaat attttcttgg
     4141 atctaaatgc agatcttcta ctggcaatgg gcgcttgtta ttgatcttca catcgattac
     4201 tacaggacga tctgattttg cagctgcatc aaaggcaaat cgaagttgcg agtattctgt
     4261 gactgtaaag ctatttgccc cgagcgcttc tccaacttta ccgaaatctg catcttctaa
     4321 aaatacacca aatttctttt gttccgtatc ttcttgttct gcttcgatga aaccaaaaga
     4381 atcattcgaa aagacaacat taataattgg aagctgatat ttgacttgtg taatgatatc
     4441 ttgcatgacc atagcaaacc cgccatcacc acttaaggta aatacttgtt tttctgggta
     4501 gcttagttgt gcggcaattc ctcctggcac accattaccc atagttgcga accatccaga
     4561 tgtcgtatgt ttttgtttac cgttcatatt caataaacga atagaatgga tagtcgtgtt
     4621 accgacatct gtgacgaaaa tcgcatcgtc ttcagcaata cgattgattt ctttatagac
     4681 agcctctgga cgaatcggtt cttcctctcg atcttcaaaa gatttcaacc aatttaccca
     4741 tttttcttta ttttcttgat ttgctaggta ccagctatca gcagggcgag catcccctaa
     4801 ctcgacaagt ctttgcaaag ctgtgtttgc atcaccgagg atcgacaaag atgtttcatg
     4861 gcgtcgtcca aattttgacg catcgatatc gatttggata aattcagcag ttggattaaa
     4921 aaatgcacgt ccaaatggga agtcgctacc gacaaataag atcagatcgg cttctgccaa
     4981 tgcttcattt ccgggtttag tagcgacacg tccagcaaaa ccaagaaagt tttcataatc
     5041 atcaggaata attccttttg ctagaacgga agcgacaaca ggaactgaaa aatgttctga
     5101 gaattctttg atttgcggaa atccgtttcg cgttccttga ccaacataaa gaactggttt
     5161 ttttgctttt tcaagataag gcaaagcagc tagcaaatct ttttcttctg gtaatatcac
     5221 acttgttttg tgagtgtgag cggtggagaa aggttgttcg tcaatttctt caaaaccaag
     5281 atcaacaggg atagtcacga tagccacgcc tttatgctca taggcggctt taatggcttc
     5341 atctactaca tgcggaagac tttgtggtgt catgactgta cgattgtata cgctaacatc
     5401 tgcgaaaata ggattttcat ttagttcctg gaaagagtta tagttcatgg atgtagaagc
     5461 aacttgtcct aagaacgcta ctagaggcac atggtccatc tgtgcatcat acaaaccatt
     5521 gatcaaatgt gtcgcaccag gtcctgcaga gccgaagaca gcgccgactt tccccgttaa
     5581 ttttgcatct gcagcggctg cgagtgcgcc gacttcttca tgacgaactt gaatgtagtt
     5641 gatttcggtt ttttcttgat atagcgcatc cataattgaa ttgaaagatc ctccgggaat
     5701 gccgtaaata tggtcgatac cccagctttc aagaactttt accattgcga ttcctgcatt
     5761 gatttttgac atggtgtttc actcctttag caatgatgat acataaattt tttgtatcta
     5821 gagtatagaa ctttttatcg aaaatcccaa ctaattcgtc ttttcacaaa aatgtagtca
     5881 tgtttttggg aatatgctag agtagtacat agatagattc aagagagggg aatggcatgg
     5941 ttgaatggct attgagactt gaaccttggc agcaagcact tgctgggaca gcttttactt
     6001 attttatgac agctcttggt gccgggttgg tttttttctt taaagaaatc aaaaaagaag
     6061 tgctaaactt gatgttagga tttgcttctg gtgtcatgat tgctgcaagt ttttggtctt
     6121 tgttagatcc ggctattaca aaagcagaag aaaatggaga tattgcttgg ctagttgtaa
     6181 gtattgggtt tggtttaggc ggtgtgtttt tatatatggc agataaaaca ctgccccata
     6241 tgcattttgg accacaacat gaagcagaag gattgcctac ccatttaaaa cggacgatct
     6301 tgcttgtttt ttctattaca ttgcataaca ttcctgaagg tttagccgtg ggggtagcat
     6361 tcggtgcagc agcaactgcg gataatccga cagccgctat attagccgct gtttcggtgg
     6421 cgctaggtat tggtatccaa aacttcccag aaggagcagc tgtatcaatc ccgttgcgac
     6481 aagaaggtct gagtcgtaaa aaggcctttg tttacggaca agcttctgga atcgttgaac
     6541 caattgctgg agtgattgga gcgctactag taacgaaagt ggagctgctg cttccttatg
     6601 cacttgcttt tgccgcagga gcaatgattt atgtcgtggt ggaagaattg atccctgaag
     6661 cacagcagac attgtccagc aaacgtcatt ttgcagtttt tggcgtcatg agtggattta
     6721 tcattatgat gattttggat gtggcattag gataaataaa aaaaatatat atgatatatc
     6781 aaatagggcc gcgaacttat atcgcgaccc tattttttta gtaagttata tcataatgaa
     6841 gtgaatggta tcatacatta ataatagttt tttctatgtg tatttccttt gatccagcga
     6901 acaagccaga tgataagtgc gattgggatt agaactttga ccgcaaacca taaaagcccg
     6961 cttaggacac tgacaaaaag actgatgacg aagctagctg caattagcac taaaagaacc
     7021 atgaagatat ttaagaacat atttttttga cgcatacaaa accctcctct tgttcattct
     7081 ttactataga aaaagtataa cagggattgt ttaaaaacac ctccaactga agactgattt
     7141 ttacttcatc tacttaaatg atcaaacaat tgctttttct ctggtttatc atctaaaata
     7201 gagcggaaaa ggaggagaat atcatgccta aaaatcgtct tgaagcattt acagatgctg
     7261 taatcgcgat tatcatgact attttagttt tagaacttca tgaacctaac ggagacacgt
     7321 gggaggcgct cgctgcatta ggttaccgat tttttatcta tattgtcagt tttatcattt
     7381 tagcgattta ttggaacaat catcatcatt tatttcaagt tgtacataag ataaatggac
     7441 gggtcttatg ggcgaacagt ttctttgttt ttgcgctttc gctttttccg tttgcaactt
     7501 catggatcag tgagtacatc aatagcctgg ctccagaaat gacttatggt tttgttacct
     7561 tgaccgctaa tattgcgtat tatatcttgg caaaagaact tgtcaaagca aataaaggcc
     7621 acgagaaagc attgaaggcg atgttgaata attacaagaa atcgtatcta tcgattggtt
     7681 tgaatgtgtt aggaattgtc ttagggtatt tagtagcgcc agtagccgtg ctgatcgcaa
     7741 acatcgtgat tctagctgct tggatcatcc caagtcgtac catcgaatca caatatcggt
     7801 aaaatgatcc gtgtttaaaa tttaagagaa aaatatagaa aaaataaata aacgatcaaa
     7861 tatccgagtg atactagtat tcaaagaggg atgcagatgg atcaaaaaag aattcatgta
     7921 ggactgatta ctagttttgt cacgatgctt atttatttgg atcaaggtca tttgacttgg
     7981 acagtagttg gtgtcgtagg atttttgaca ttattcacag caataaaaaa ataaacatct
     8041 ttatgtaatc tgatgttcta tctatttaag aaaagcatgt ttacagcagt atgattctgt
     8101 tgtaaacgtg tttttatttg tggaaaattt aatttttaga aaagtgtatc gaaaaataga
     8161 tagttagttt ttccacagtg gataacttcg taagagataa aaataaaaaa ttagtcaaag
     8221 aagggcgtat agatttcttt tgataggaga tatacaacag aaatgaataa atattaacgt
     8281 gttttcaaac agtataaaac ggtgtggttt ttggaataaa aaaataacat atctcatttt
     8341 acacttttgt tattttatac ttaaaaaata acgacacaga aaataccgtg tctcagattt
     8401 ctctactact tctaaaaact actgtttttt tcctttgaaa tgccttcaaa aagtaggatc
     8461 aggcagaata tactggcaga atcaaggaga agggagccta aataaggaga aaaaccatag
     8521 aaaatataaa gtaattactt ataaaacaag ctttttatag aaaaataata tacctgagta
     8581 gggtataaat taagacggaa ttcctttcta gattgagata ttgactagga agaatgttaa
     8641 gattttaagc ctttaagcta aaaaaaaacc ctttttgtta aataagggga atatagcctg
     8701 tcagtttcaa actgcaagtc gtcctgctgc aataatatag tagattagag atgaaaatag
     8761 atttttttaa accaatgact gaataaaaag accgtcatag atacacattg gaatgtgtct
     8821 attacggtct ttttttacga acgattttat tgaagacgga cagttcgggt caaaaataaa
     8881 aaagctacga tcagaaataa gaataaaatg tatcccaaaa taatggctag acggtctaac
     8941 ttatttgtcg atagatgttt gtttagaagt tgataggccc ataaacctat cagtcctcct
     9001 gttgtattag ttatcaaatc agtgatgtcc gctacgccaa taccgaatag gaattgaatc
     9061 aattctgcca agaagcttac aaggaaaata atccctaatt ttttagaaaa agaaagtctc
     9121 ttgaaattga cattcaaaag caccccaaaa ggaaaaaaga caatcaagtt ataagtggtc
     9181 tcacgtaata ccgtcgtatt ttcaaaagaa aaagtagaaa aagggacgag attgacattg
     9241 ctgtaagaat aagtgagaaa ttgtggaaga ttaaatgata gcttgaataa aatcaaccat
     9301 aatagaaaga ataaataaat ccctaataaa aattttgata cataccgcat agacatctcc
     9361 tccatttaaa aagtatacca tcgaaaaccg ctgaaaaatc ataaaaaagt atgaagttta
     9421 aggaccaacg tagttatcgt acgaaaatta gaagcttttt tttgttggga atccggtttc
     9481 tttaacaaag ctaagatagc gagttgcttg gaaaagttgc tatctttttt ctgtcaaaac
     9541 gactcggaat gttcgttttt tgttctaatt tctccaagtt gtttactttt tgatactaag
     9601 tttatgaaaa atcaagaaat agttgttata ctaggtaatg ctgacaaacg ggattggcat
     9661 ttcatcttgg ttcagctttt tttccaatta ctaggaagga gggatagatt gttcaagact
     9721 tattttcgtt tcaataaaat acaagataat ccttttcgta tcatgaatct gatgctactt
     9781 atctttattg tattatggtt aggttacata aatcatgatt ttttgatttc aagtttttca
     9841 tcattaggga tctttacttt tttctattat caaaacatac cgatgaaata tttgctgaaa
     9901 cgtatgtttc tgatcggttg cggactgttg gttgctttcg ttttggggat cttatcgact
     9961 tatgttttat ggcttgaacc atttgccgta gctttggttg ctttttccag cagattcgtg
    10021 ttgcgattat tccatatttc taaaccaggt ggcttgtttt ttgcgatgct ttctgccatg
    10081 ggaacgagca tgcagctgcc aatcgctcaa ttaccgatcg tctcacttta tttttttatg
    10141 ggtgtcgttt ttgccctgat tgcggcagtg attacaaaat tactggattc acggcctgag
    10201 cagacaattg aaaaagcaac tttaaaagaa agatttcacg aagagccttt agtgatcatt
    10261 gattctgttt tctacagcgc tgccttattt ctttccgttt atgtcagtca cggcttgaat
    10321 ctgcataatc cttattggtt gaccttgtca tgtgcatcga ttttgctagc agaaaacttg
    10381 gatgcgatga agcatcgaca agtccaatat ctgattggat cgatgggtgg gttatgtgtg
    10441 tcggcattct tgtcatttgt gccgtttacg cagttgcaga ccatttttct gatcactttt
    10501 ctatatggaa tcgcacaatt tctagttgct agaaattatg cagttgctaa tatttttctt
    10561 aatccgatgg cgttgatgct atctacactt attcgtggag catacttgat ttctttgatc
    10621 gaatatcgtt ttttagggat cgtcatagga agtttcatcg gtttaggtgt tgcgtgggta
    10681 atgacggttg gtttgcaaca ttatttaacg gttgttcgtc aaaaattaga atagaaggac
    10741 aaatatatgt gccaaaagag gttatgacaa gatttttgtc acaacctcta attcaaggtg
    10801 ttcaaaagcc agattctcag cattaagtca aattcacaca aaaatgtgga aaggaacttt
    10861 caaaaaaatt ttatatttat tctatcaaaa aattttgttt atgatggtaa ctgaataaac
    10921 tttcctgaga tggtcaatgc tttccttgaa agtcttacag atattctttc ttcatagaaa
    10981 gtttaagttt tatagttagt gtacaataaa agtatgttta taccatatgg tgtccggata
    11041 atcagtcatt taaacaatga ggttcaaaga tatgaaaagg ggaaatttga tgaagaaaat
    11101 aagcaatgaa atccaaattc tggcagaaac tttaactctt cccaaagtcg ttatagagca
    11161 aatgattgta caagcaaaaa acatccttga tgaggtgttt gagcaatata gtcatgaact
    11221 aatggatcct aataaagcaa aggcgactgc aaagaaaata gacaaagtct atgaagagga
    11281 accttttgca gcattagcta tttatctata tgcagcttct caaagttgga aaaaaattta
    11341 tcaaccattg aagattccaa gaactatcta tttggcaaca atgaatgctt ttactagatt
    11401 tattcatgaa catttccaag taacaggaag ctatcgcttt gaccgtggtt tttggatttg
    11461 gcattacact agtggcttga tctttcggat tggtgaattg gagtacgaaa tgacatattt
    11521 tccgaaaggg tacacggatc taagattaga aggaaagcag tgcctttctc ttcatattcc
    11581 ttccgatgca gatttatctc agaataagat aagagatagt tacgaaagtg ccattcgttt
    11641 ttttgaactt tattttcctg attaccatta ccaagtgatg tacacagata cttggttact
    11701 atcacccaat cttactaaat ggttgaaaaa agagtcaaaa atctgtcttt ttgcagccga
    11761 ttatcgatta ttatcagttg atgaacagga cgatagtgga gtcccttgga tctttggacg
    11821 agtagatgcc caaatacatg attatccaga aagtacgagt ttacagcgac aagcaaaaga
    11881 gcaattgctt gcaggcgagc acattggctc tggcttaggt attcttactt cataagttta
    11941 gtacttcgaa atagtttaaa agagcgtgag acaaaagtaa agaacacttt tgtctcacgc
    12001 tctaaacacg tataaacggc ggaagcagaa gcaactcctt cggaaataag ctgaaattca
    12061 caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct
    12121 gtcccagcct cttttttagt gttctatcga acacacagta cgttcaatag gtaaactagc
    12181 tataacaata gatattaaag ttaacggaca agagggcctt ttttaggtag ctaaaacgta
    12241 ataatgaaaa tttatttcca gagataggag tcaatcagct agtctgatcg actgttttaa
    12301 caagttgact ttcattttaa cttttgatac catttaaaac acaaatcgta attgtttcga
    12361 tttgtgtttt ttagcatgac tacaatcaaa gaagaaagtg ggtcgtccat atggaaaaaa
    12421 cagatatctc aagtgcatac cgccgcttga aaagcccaaa tatcaaaaca cgaaaacgtg
    12481 cgctgaagat catcaaagat gtgaaacgga atagtgggaa aaggtaagaa gaaggaaact
    12541 taattaaaaa aatagccgaa aaacgttttt cggctatttt caataatcgg tttttgtgta
    12601 aggtttccga aagaactgct gttgattttc catttttaac atatgataaa ttggtagaat
    12661 ctttcttttc aaattgatac cattccctaa ttccagttat atagttaact acagaaccac
    12721 aaagcacgta cgtttataaa agtattatct gtagttattt ttcaattccc tttcaacctc
    12781 ttattttgac cagtagtatt gatcacttta ccttttaaca cctttgttcg tttttgaaac
    12841 atttttagtc aagaatggaa acgtaactac ataatcaaag gttttgcgaa atatttcatg
    12901 aaatatttca tgtagaagcg agcaatacgg ttgcaaataa ccattatcgt ttaataagca
    12961 gttacgaaag ctcccaatat ctctaacgtt cagctcactc catttctttt cttcaaaaaa
    13021 atcagtgatt cgtatctctt tctatttgtc gaaaaagtta actttaaaaa gtaacgccga
    13081 aaaaatatgc aaaaaaataa aaaagcagtt actattgcta gaataacatc aaggaataat
    13141 aagttttggt tttcagataa tagtcgataa gttgtgtatt ggaattttag atacagtaat
    13201 aaagtatttt tcatgaaatt ttgtgagaat cactattaca agtatcttta ataaaattaa
    13261 tggttaagga ggaacttaaa gtagaatgta aatgaatagt tgtagtcagt cgattgtgat
    13321 actagactca aagttgcagg aataagtttt tcaatttgtg attggaagat aaaaaatgaa
    13381 aaagaagggc ttttttttat actttgttga ttgtagtgtt tattttccct ctgtttagcc
    13441 aagtaacatt ttctatgact caaccagttt caagtgagga acatgataac ctttttcgtg
    13501 ctacagaaga gtttaagaaa tatcaggaaa atattgttcc ggagactcca ttagctagtg
    13561 agccaattac tgatattgat ggaaaccaaa taggatggta tatacagtac gaagttatgg
    13621 tggtaaataa tgactcaata gtaaaaaata cagtaggctt agagctaaca ccgattgaaa
    13681 aggtgagttc attgttaacg tatatttata ctgatgaaag tgacgagact gagatcattt
    13741 taacggatta ctcaaaaatt gctactgata aaaagtatat attgtggatg tagacactag
    13801 tgaaatactt tatgatatag aaattgatga aaatttacta gatgtagcac aagaaatcga
    13861 tcatcaaaag caggaagcta ttcgatatgc tggtagaaaa gggaaaagaa aatatttatt
    13921 acgtgaatgt agcaaaagtt agagaagatg aaaatgagtg gaaagaattc aagagtcgct
    13981 actcaattaa ctcgactccg acatttacag tttacagaga aggatcgata gaaaaaactg
    14041 ttttttggac taaagaaagt ggaatgtctt tagctgaagt agaagagttt ttggattatg
    14101 ttagtatgca acagtaaggg agttcttatt atgaaaaatt ttgacacata ttatgttatt
    14161 gttttttcga tatctttgct gttaatatta ctaactttaa tgcagtataa agagtatcca
    14221 gagtatttta ttttaacttt tgtatattta gtaagtagct acacacttct atataaattc
    14281 agaagttctt ctagagtttc taaagtttgt ttcttaatta gcttattgat tctataatat
    14341 ttttgattta tataggaaat taaaaatatt atagaatcaa cactcagaga acgggttggt
    14401 ttttaataga tttaataagt actgataatg aaatttctcc tggttgggaa aacgaagttg
    14461 gttaccctgt agaagaattt tttcaagatt actttgcgga gtatgccgat aggataatga
    14521 gagattacgt aacagataaa actcaagaag tcatcaatca atcccagtca ttaaatgaat
    14581 tatctgataa ggcgaaagaa gaagcagttg ccattatttc ttcagggatt agtgactata
    14641 ttgatatgag tggtgaaatc aattatggcg ataaagacaa aattgtacgc atgataaaag
    14701 cttttatcaa aacttttgat gtgaaggcag atcttaagtt taatgatact tacctagtag
    14761 gaaaattaat taatatcctt gggacagaga aagatacgga aacgtttatt tcctctttgt
    14821 ctatgttgtt tacagataag catttttcaa ctcagcttgg tggagaggaa ctagtggaaa
    14881 tcttttaacc attacgccca gcattaataa aagtatatca aaaaagtact cctgatgatt
    14941 tttaagattg gtttgagaaa taatgtgaaa acactatttt taatgttaat tatagataga
    15001 gatgaacgcg tcatatggtt aaaagaatga aaaagtcaaa gatacactgc tatatttcac
    15061 taaggatata gcagtgtatc tttcatgtcg tttcactggc agttttttat aaaaggaggt
    15121 gtgcatttca gttgcttttc tttggtagtt agttgaatcg tttaagaatt aacggatgtg
    15181 gcagacaggt gctctgtcca aaaaccagta aaatctttga tggttccatc tttatttagc
    15241 tgtgcctgtt caaatactgt tttgttttta ccgaatggat caattgcaat aattgttccg
    15301 atccccgcac gtttggcagc aagtaatcct gagtaagcat cttcgataac aagacaatca
    15361 gccggagcta agctcagttt gtctgccgct ttaagaaaaa tatccggttg cggtttccca
    15421 gggaaagagc cgtcgtcata gacaatcttg tcccagtcaa accagcgggc taaatcgaaa
    15481 atatcaaaat aaaaagcaac attctctttg acagtagctg tggcaatcgt taatggaatc
    15541 tgttggtcag tcagctggtc aagagtcgct tcaagccctt ttgtcaactg aagctcgttt
    15601 tcattttgca agcaaagcat acgataatag ctttcttttt cttctgacaa tacggcgatc
    15661 tcagaggcgg tcaaatgctc tgaaatgaaa tgtttcaaaa tctccgtatt cgtccgacca
    15721 tgaatatttt ttaaaatatc ctcgtctgat aaaccatctt ttgtatgatc atgtatcata
    15781 tgcagccaag cagcttcatg cagatgagag tccaaaaaca ttgttccgtt gaaatcaaat
    15841 ataatccctt tcatagttac gctcctatag tggtattttc ggttgaatca gcaatttgca
    15901 gatcgacata ttgtgcatag tgctcttgaa cctcccgcga gatctggcga tccgtaatga
    15961 gcagatcgaa atcatgcaaa ttataaaaca cataaaaatc atctcgattg aattttttat
    16021 tatcaacgag tagatatttc ttacgggaat tatttagagc gatctgctgt gcttctcctt
    16081 cttcggtact gtaagtagag atcgcttcgt tgtgaacgcc atttgaactg ataaatgctt
    16141 ttgtaaactt tagtcttctt aaattgtcgt tggtgatcgg tccgacaaat gcgccggtat
    16201 ttttccggaa atcaccgcca atcagtagaa tttctggtcc atctgcttga gcaagaatat
    16261 caaaaactgg ataattattt gtaatgatcc gaattttctt gttcagtaaa tgcgtcgcca
    16321 atagttcgat tgttgtacct ggtcctaaga aaatcgtttc tccctcgtga atcaaggagg
    16381 ctgctgtggc agcaatttct tttttttctt gaatctgcag agtggctttt tcagtgtgag
    16441 aaagttcttg atctaaagtg taggaaagac tttgagcccc tccatggaca cgcagcagct
    16501 tgcccgattt ttctaattca tctaaatctc tgcgaacggt catatcagaa acatctaatt
    16561 gattgatgat ttcattaacg gtaaggattc cttttttatt gaccatttct acgatagtaa
    16621 gcaatctttc ttttttcaac attgtcttca cctctaattt aaggataatc tatcatataa
    16681 acggacaaca gtcaatctaa ttcgaacaaa agtaaacatt tagggtggtt tttatgtttg
    16741 ttacaataga ttttattgct gttttctggt ttttatgatt atttgtgttt gtttttgttg
    16801 acaaatgttt tggatgcgtt tacaatgcaa tcagttagaa agagcgaaaa ctgattatag
    16861 atttgaggag agatgtacat gcttaaatta ccagaagatt ttatttttgg cggagccaca
    16921 gcagcctatc aggttgaagg agccactaag gaaggtggca aaggcgctgt tgcttgggat
    16981 gactttttag aggaacaagg gcgattcagt cccgatccgg caagtgattt ttatcatcaa
    17041 tatgcaaagg acattgaatt gtgtgaacgt tttggtgtaa atggtctaag attatccatt
    17101 gcttggagcc gcatttttcc tgatggagct gggaaaccaa atccagaagg aatcgctttt
    17161 tatcatcgtg tgttcgaaga atgtaagaaa agaaacgtaa cgccgtttgt gacgttgcat
    17221 cattttgata cccctaaacg tttgtttgac catggagatt ttttgaatcg agaaacaata
    17281 gaagcatttg tttcctatgc cattttttgt tttcatgaat tcaaagaagt caaggtgtgg
    17341 agtaccttta atgaaatcta tccagttgca accaatcaat atttgttagg tgttttccca
    17401 ccaggaatca aatatgattt tacaaagatc gtcgcctgtc ttcataatat gatggttgct
    17461 catgcccgtg tagtgaacta tttcaaagaa aacgaacttc cgggtgaaat tggtgtagtt
    17521 cactcattag agacgaaata tgcggcaaca gacgcgccgg aagataagca tgctgccttt
    17581 ttagacgatg cattgtcgat ccgtttctta ttagatgcta cctatttagg ctattattca
    17641 actgaaacct tgactgctct tgatgagata tgtgaagcaa atcaggccag ctatcatttt
    17701 cctgaagaag atttcgttga attgaaaaaa gcatccacgc gtaatgacta cttagggatc
    17761 aatcactacc aatgccattt tgtcaaagct tacgatggag aaaatgcaat ccatcataac
    17821 ggtacgggtg aaaaagggac ctctgtttat aaagtgaagg gaatcggaga acgtatttat
    17881 aaagaaggaa ttcctcgaac tgattgggac tggctgattt atccagaagg tctatatgac
    17941 ttgttgctgc gaattaaaag cgattaccca cattacaaca aaatctatat cactgagaac
    18001 gggatggggt ataaagatca gtttgaagat ggcattatta tggatcagcc gagaatcgac
    18061 tatttgagag tctatttgga atccttaagc aaggcgataa cagccggggt aaatgtgaag
    18121 gggtattttt tgtggtcgct tatggatctt ttctcatgga caaatgggta taacaaaagg
    18181 tatggcttgt tttatgttga ttttgaaacc cagaagcgtt acccaaaaga atcagcttac
    18241 tggtacaagt tggtcagtga aaccaaaacg attatttaat aagtgataat ggaggaagaa
    18301 aagatgaata aagaggaatt acaaatgctt ggttttgaga tcgttgcata ttcaggcgac
    18361 gctcgcagta cattattgaa attattgaaa gaagtaagac aaggaaactt tgaacatgtt
    18421 gattcggcgt tgaaagaagc cgacgaaaat ttaacccttg cacataattc gcaaacaaaa
    18481 attttagcgg aagaagcatc tgggaaagag atggagatgg gattcatttt tatccatggg
    18541 caggatcatt taatgaccac tttattgctg agagatctga ttcaagactt tattgtactg
    18601 tacagacaga atcaaggata ggagaagaac gctatggatg gaatcattaa acaaatcgaa
    18661 aaagggaaac cattttttga aaaaatctca cgtaatagtt atttaggagc cgttcgtgac
    18721 ggatttttaa cagcgatgcc agctatttta ttttcaagca ttttcatttt gattgctgcg
    18781 gttcctgaga tttttggtta ccaatggcct gaatctgtct ccacatggct atggaaagtc
    18841 tataattatt cgatgggtgt tgtagggtta ctcgtttcag caaccactgc tcgctgcttg
    18901 gctgattcga tgaatcgtcg cttaccgaaa aacaaagtaa tcaacaccac ctctgtgatg
    18961 ctggcctctg tggtaagttt cctgttgtta agcgtctctc cgattgaagg tggattttca
    19021 acggaatata tggggacaaa gggactactg gcttcctttg tcgctgcatt tttaacagtt
    19081 aatttatata agttttgtgt gctgaaagat attaccatta aaatgcctaa agaagttcct
    19141 ggaacgattt cgcaaacctt ccgagatatt tttcctttct ctttttcagt atttgcggcc
    19201 gttttaattg atttaggcat tcgacaaatc ttccaaatct catttgcaga agcaatcatt
    19261 acggtgctgc agccgctatt tacagcagct gacgggtact taggaatcgc tattatctgg
    19321 ggagcgatgg cgctgttttg gtttgtcggt gttcatggac cgtcgatcgt ggagccggca
    19381 attgcggcaa ttatctatgc caatgtggaa accaatctgc aattgttcaa ttccggtgaa
    19441 caagcttcta aagtactgac ggtgggttta gggaattttg taggaactat gggaggaact
    19501 ggggcaacat tggttgttcc ttttatcttc ctactctttg ctaagtctaa gcagttgaag
    19561 gcagttggga aagcttcctt tattccagta agtttcgctg tcaacgaacc attgctattt
    19621 gcggcaccca tgattttgaa cccttatttt tttattccat ttttgttaac acctgttgta
    19681 aacgtttgcc tgttcaagtt ttttgtcgat gtattgggca tgaacagctt tatgtacgtt
    19741 ttgccttggg caactccagc accgatcggt ttggttctag gaacgggcat gggtatctta
    19801 gcttttgttc tagcagcggt tctaatcgtc gttgacttcc tgatctatct accattttgc
    19861 aaagcatacg atgctgtctt agtagagaaa gaagcacaaa aagcagaggt gatcgcaacc
    19921 gaggaaggtg cttctgccga tgtacgtgag gaagtacctg tggcagcgag tcaagttatc
    19981 agcgatgcaa ataaagaaat caatgtgttg gtactttgtg ctggcgcagg gacaagtggg
    20041 atgttagcta atgctttaaa aaaaggtgca tctgaatcga aagcaccgat cagtgcaatg
    20101 gcgggtgctt atggttctca ttacgaaatg ctgcaggatt ttgatatggt ggtcttagca
    20161 ccgcaagtca attcttacta tgatgatatc aaagaagaca ccgatcgctt aggaatcaag
    20221 ctggttgcga caaaaggagc agaatatatc ggattaacga gagatcctga aaaagctgtt
    20281 cagtttattc tgaaccaatt tcaataaagg aggagccata atgaaaatgg gaatttggtt
    20341 gcgtaaagct gttttatttg cagctgtgtt aggggctttg ggggctgttt caaaaactgt
    20401 tgaagcatct gccagcgaat ttattcgcgg agcagatatt tcgatcctgt cagatatgga
    20461 aaaatctggt gcaaaatatt atgaagacga tatttcaaaa gatgccttag agattttaaa
    20521 aaataatggt gtcaactatg ttcgactgcg tttgtggcag gatccttata atagtaatgg
    20581 agaaagttat ggagcaggga caaatgatct ggaaacgacg attgaccttg caaaacgagc
    20641 aaaaaatctg ggacttaagg tgctgctgga ttttcactac agtgattttt gggttgatcc
    20701 tgggaaacag aatcttccta aagcatggca aggtctgacc tttgaagaaa tgaatacggc
    20761 attgtacgac tatacaaaga acgttctgtc ggaaatgaag cagctggacg tttatcctga
    20821 tatggttcag atcggcaatg aactgaatag cggaatgctc tggccttatg gcaagtcttg
    20881 gggagaaggc gggggagaat ttgatcgatt ggctgctttt ttgaagtctg gtatccaagc
    20941 tgttcgagac actcaaccta aaacaactcc agtaatgctc cacttagctg atggtggcga
    21001 tacaggagca tttacttggt ggtttgatga aatcacgagt cgaggtgtgt catttgattt
    21061 aattggagtg tcgtattatc cgtattggca cggttcaatg aatgaacttc aaaccaatat
    21121 gaatgcgatc agccaacggt atggtaaaga agtgatcgta gttgaaacag cttatgggca
    21181 tacaacagcg aatgccgata ctatgccaaa tgcttttggt gaagccgaag cagctgcggg
    21241 gggatatgaa ccaacaccga caggacaagc agagtatttg ctggatcttg ccgatcggat
    21301 tcaggcagta ccgaataatc gcggggcggg cttcttctat tgggaacctc tttggtacaa
    21361 cggaaatgtg agttgggcaa cacaagcagg tatgaattat ctcagtgttc aatccactat
    21421 gggaaacgaa tgggacaacc aagcgatgtt tgattttgct ggaaacgcct tgccaagctt
    21481 gcgtgcgttt aaacaagcag gggcgcaaat aaatctagtt aaaaatgcaa gtttcgaaag
    21541 cgatggacca acgaccagtc cttccaattg gcaaacctgg tttcaacaag gaaccgctaa
    21601 gggtacgatc aaaacggaag cgggcacttt ttttggggac tacaaactaa ctttttggca
    21661 ggatcaagca tacgaggcat cagcttatca aaatgttagt ggcataagca aaggaactta
    21721 cacattgagt gcctatgtaa tgtctggggg agagtttgat actaatcagt tatatatcaa
    21781 aaattatggt ggagcggaac taaatactgc ggttgttaaa acaagtgaat ggacaaaaat
    21841 tacaatcaaa gacattccaa ttacaacagg tgtttgtgaa attggggttt atagcaaggc
    21901 ttcaggaaat gcttggacga acgttgattt tgtttcattg gtgaaacaat agactgcgga
    21961 tcgtttcgat tcaagatcgg aggttccctt cagccacatc gttagcgtag aaggcaggaa
    22021 tcgaacctgc acctcgcctt tgccaaaaat aaccagaaac gatttccagt catttttacc
    22081 aaaacgagta aagcaccaga cttcatctac actttgtatt atgctaaaga gagttcaacg
    22141 atttcgtaag aagcaatgaa aaaattgcaa aaagttgtgc agataacata aagtttgttt
    22201 aagaaagggt aaccttagaa ttccgtcgtg aatatgattt gagcacttag atggatagaa
    22261 aaaagttctc ggaaaacagt tcgtttttcg agaacttctt tttatgataa gaatgaaaca
    22321 aattaactta tattatagtc tcttttggta gggaaccatc ttatttttaa tgaaaatcaa
    22381 gcattttagc tattgaagaa tgttgattta tagggattcc ccctccatat tatgttggct
    22441 ctttattccc actccactgt tgctggtggt ttagaagtga tgtcatacac gatacggttg
    22501 acgtgggcta cttcgtttac gatacgtaca gagatttttt gcaagacttc ccatgggata
    22561 cgggcaaagt cagcggtcat tccgtcgatt gaagttacag cacggatacc gactgtgtag
    22621 tcgtaagtac gtccgtcacc cataacaccg acagagcgga tccctggaag aacagtgaaa
    22681 tattgccaga tatcacggtc aagtccagca gctgcgattt cttcacgcaa gatagcatca
    22741 gattcgcgta cgatttctag cttgtcttca gtgatttcgc caagaacacg aataccaaga
    22801 ccaggaccag ggaatggctg acgccaaacg attgaatcag gcatgcctaa ctgagtccct
    22861 aattcacgga cttcgtcttt gaataatgtg tttaatggtt cgatcaattc aaattgcata
    22921 tcttctggca gtccacctac attatggtga gatttgatcg tctgtgctgt ttctgtacca
    22981 gattcgataa cgtcagtata caacgttcct tgtgctagga aagagacgcc ttcttcacca
    23041 gcaagttttg ttgcttcgtc atcaaaaaca tatacgaatt cattcccaat gattttacgt
    23101 ttttgttctg ggtcagatac acctttcaac ttgtctaaga agcgatctct tgcattgact
    23161 ttgatgatgt tcaagccgaa ttttccgcct agactttcca ttacttgttc cgcttcgcct
    23221 ttgcgtagca gaccgtggtc aacaaagata gatgtcaatt ggtcaccgat cgctttttgt
    23281 aaaagaaccc caacgacact agaatcaacg ccacctgaaa gaccaagcaa gacttttttg
    23341 tcaccaactt gttcacggat tttttctact tgcatatcga taaagttatc cattgtccag
    23401 ttaccttcac agtgacaaac atcaaaggca aagtggcgca gcaattcgat tccgtattcg
    23461 gaatgacgta cttctgcatg gaattggata ccataaaaat tacgtgttgt gtcttggata
    23521 gaagcaatcg gacaatcttt gcttgttccg actgtttcaa agccttcagg aactttagtt
    23581 accaagtcac catggctcat ccatactgtt tgttgttttg gtgtttcttt aaacaagaca
    23641 gcttgatcgc ttgttacttc taacaaggct ttcccatatt cgcggttttt agcaggctct
    23701 acggaaccgc caagtttgta cgtgatcaat tgcataccat agcaaatccc taatacagga
    23761 atacctaaat tgaagatctc aggatcgatc ccaaatgcat tctcatcata tacactgttt
    23821 ggtccgccag aaagaatgat tccctttgga ttcatcgctt ttacttcttc tgcagtaatg
    23881 cggtggctca ataattcgga aaagactcca agatcacgaa tacgtcttgt gatcaattgg
    23941 ttatactgac taccgtagtc taatacgata atcttttcaa cgttcgtcat atcggcaacg
    24001 tttgtcaaat ttattacccc tatcccttta aattttatct caaaatagtc aaactatttt
    24061 aaaatgctaa tattttcgca tgaaaatcga atcgatgata tgattttcag ttttatggag
    24121 aattatatcg gctcttcctc gtgtaggcaa gatatattcc tgtaaatttt tcaaattcac
    24181 atttttccat acatcttttg ccattttaaa ggcttcttca cgatctccga tagcaaaacg
    24241 ataataataa ttctctggat tttggaaagc cgtatccagt aacgcaccaa aacgatccaa
    24301 ataccatttt tcgatcagca cgggatcggc atcgacatag acagaaaaat caaaaaagtc
    24361 gctgacgtag atttgctgat tcgcaggtaa ttgaagcgta ttgatcccct caacgatcaa
    24421 gatatcaggc tgctggatca attcgtactt gtctggaatg atatcataca catcatgtga
    24481 gtaaacaggt acttttatct cttcctgacc agattttaca cggttcaaga aatcgatcag
    24541 catctccata tcataacttt caggaaatcc tttacggtcc atgattccac gctcttttaa
    24601 tatacgattt ggataaagaa atccgtcagt cgtgatcagc tgaacgtttc ttcgtttgaa
    24661 ggtacgagac aaaatcgtct gcagcagtct ggctgttgta ctttttccaa cggctacact
    24721 tccggcgatc ccgataataa aaggcggtac ttctacgtat ttatgaagaa ataaaccttt
    24781 actcaagaac aaagattcaa attctttcat ataaagataa atcagatgag caagaggtac
    24841 atagatatct cgaacatcct gcatggaaat gcggtcattc aaacttttga tattatctag
    24901 ttcatcttgt gtcaaaggtg cttgcccatt acgataaaat tctcgccatt cttctcttgc
    24961 cacacggtag tagttcattg gatcattcat attagcctcc tgcgttctcc catcaatgga
    25021 actattttaa cacaaaataa acgcaaaccc caccacttaa gagagattgc cgatcttttt
    25081 gtataatctt tataatagaa agaaacggag tcttattctt gacttgcgca cggatacata
    25141 aaaggataga ctgttgtcat gaaagaaatg agaaggatgg aagacatgac aaatcattac
    25201 tattctgaac gcccagatac ggcgcacgat tttgaacaat gggcttttga gttaaaaggc
    25261 agaacctttc aatttgtaac agattctggt gtattttcac gtgaaacagt ggattatggt
    25321 tcacgtgtcc tgattgatgc tttcgattgg gaagagttac ctgatgaagg aaagatctta
    25381 gatgtgggat gcgggtatgg accgattggg ttagctttgg catttgcgac acagcggttt
    25441 gttgaaatgg tcgacattaa tgaacgagcg gtaagtctag cgcaaggaaa cgcaaagcgt
    25501 aaccaaatcg aacatgttga tatccatcaa tcgaatatct atgaagcagt tcatgaagaa
    25561 acctatgcag ctatcgtaag caatccgccg atccgtgctg gaaaaaaagt tgtacatgaa
    25621 attttgacag gcgcatatcc tcggttgaag aagggcggta cactgacaat tgtgatccag
    25681 aagaaacaag gagcgccgag tgcgcaaaag aaaatggaag aaacatttgg taatgcagaa
    25741 atcgtcacaa aagataaagg atattatatc atcaaaagtg tgaaagaata actagctgaa
    25801 aaatagtgga gcgacgaaag agtggaactg ggcgtgcatg tacgggtacg tatcagttct
    25861 ttttttgttg agaagttttt aaaaacaaag aactattttt tcttcaaaaa gaaatagtag
    25921 gaacagagtc gttgtttttt acgataaaat aaataagata cagaacttgt atagaaggct
    25981 gccaagtcgg tagataaagg aggtttttat caaaaaacat actttccttt tttctctatt
    26041 cttttcttgg gggttttggt acactgatag ggaatttaga tagaagggag ccaataggat
    26101 gaagctgacc atcagagata tcgcagaaat ggcaggcgta tcagtaacaa ctgtctcaca
    26161 gattttgaat aacaaaggaa gcagatttag tgataagaca agaaaaaaag tgttgtcgat
    26221 cgtgaatgaa catcattata agcccgatta tttcgcttca aatgtgatca atcgacattc
    26281 aaagaccatc ggaatgatcg tccctgatgt cactgatttc ttcttctcca aactgatcga
    26341 aggtgtagag agctatttga atcctttggg ctatgtgatc atgttatgca attctcgtca
    26401 tagccaagaa aatgagatca agtatttaca agagctttcc caccgctcag tggacggaat
    26461 cttgctggcc acaccaaata ttttacctga gaaatatgca ttagacagcg gtttttatca
    26521 aaaaatgcca atcattttaa tcgatcgcgg attgaatcgt cgggataacg gacggcttat
    26581 cgtaaaggaa tatgagggcg cctatcaagc tgtttcgctg tttattgaaa atggccatac
    26641 gaagatcggc atgctgaagg aaaccactgg atactatcaa ttagaagaac gtttcaacgg
    26701 atatcgtcat gcgctgaaag atcatggcct gccttttaat gggaaatttg ttgaacatgg
    26761 agagttgact gtccaaggtg gatatactgc atcaaaaaaa ttactcaaac ataaagatat
    26821 cacggctatt ttttgcggga atgatgccat ggcgatcgga tgctatcagg cgatcgatga
    26881 attgggcaaa aaaatcccag aagatatctc agtcatagga ttcgatggat taaagctgtc
    26941 agaatacatg attccgcaat tgacgacagt gcagcagcca agttttgata ttggatttta
    27001 tgctgcacgt ttcctgatcg atacgatcga atttcctcaa agaaaagttc cgaacaaagt
    27061 gttcgaaaca aaattaatta ttcgtgagag cgtaaaagta ttgacaaagg gctaactcgg
    27121 cggtatattg cttatgtaag catttacaaa ggctagttag tgcttttttt atttttagta
    27181 aaccagttta ctaaagtggt ttacctttcc taaaaataat gtataatacc gaatgaagac
    27241 aaggaggatc aggcaatgcg aatggtggat ctgattgaaa aaaagaggga tggaaacgag
    27301 ctgtcgaaag aggaaatcga gtacatcgtc acgaactata cgaatggaaa gattccagat
    27361 tatcaagtca gtgcattatt gatggcaatt ttttatcaag acatgacaaa tgaagaaatc
    27421 acgaacttaa cattggcgat tgctaattct ggtgatgtca tcgatttgag ttctcttgaa
    27481 ggaataaagg tagataagca ttcgacaggt ggcgttggtg acacgaccac attgatttta
    27541 gcccccctag tagctagtgt cggagtgaca gtggctaaaa tgtctggacg cggtctaggt
    27601 tacacaggtg gtacactaga caagctagaa gcaattcccg gatttcagat cgagctttcg
    27661 gatgaagaat ttgtacgcat tgttaatgaa agtaaagtag cggtaattgg tcaatcagga
    27721 aacttggcac cggcggacaa aaaattgtat gccttgcgtg acgtcacagc gaccgttgat
    27781 tctcttccgc tgattgccag ttcgattatg agtaaaaaaa tcgcggcagg agcagatgca
    27841 atcgttttgg atgtgaccac tggagacggt gctttcatga agaacatcga agatgcaaga
    27901 cgtctggcaa agaccatgac gagcattggg aaactggcta accgagaaac agtggcggtt
    27961 atctcagata tgtcagaacc tcttggcgaa gctatcggaa atagtctaga agttgtcgag
    28021 gcaatcgaaa cattacaagg aaatggtcca gaagatctag tagaaatgtg ctacgcctta
    28081 ggaagccaaa tggttgttct tgctggcaaa gctaagacaa ccgatgaagc aagaacgttg
    28141 cttcaagaag ctctggaatc aggtaaagcg ttagcaaaat tcaaagaaat gatccaaaat
    28201 caagggggag acccagcgat cgtggaacat ccagaacgaa ttttgactgc acgctacaca
    28261 atggagcttc ctgctaaaca aagcggagtt gtgtcaaaaa tcgtggcaaa tgaattagga
    28321 attgctgcaa tgatgctagg tgctgggcgt aaaaccaaag aagaagatat tgatcacgct
    28381 gttggattga aactgcataa aaaaattggt gacactgtga caaaaggtga gtcactattg
    28441 acgatctata gtaacgacga agaaatcact tctgtcattg aattattata taaaaatatt
    28501 gaaattggtg aatcggcaat gaagccaacg ttgattcatg acatcatcac agaataaagg
    28561 aaaaaagcgg actttaccgt tttaaagaaa aggggaaaag aaatggaact aaaccgaatg
    28621 atcgaccata ctattttgaa agcagacgca tctaaagaag ctgtgatgca aattatcgaa
    28681 gaagcaaaaa aataccattt ttattctgtc tgcatcaatc cagcatgggt tgcgctagcg
    28741 aaagaacaat tacaaggtga gccagtagcc gtatgtacag taatcgggtt tcctctagga
    28801 gcaaacacac ccgagacaaa agcatttgaa acaacagatg ccatcaataa cggtgcagat
    28861 gaagtcgata tggtcatcaa tatcggtgct ttgaaatcaa aggatgatca acaagtccaa
    28921 aaagatatcg aagcagttgt tgaagcagca aaagatcgtg cgcttgtcaa agttattatt
    28981 gaaacatcgt tattagatag agaagaaatc atccgagctt gcgaaatagc aaaagctgca
    29041 ggcgctgatt ttgtcaaaac atcaactggt ttctcaacag gcggagcaaa ggtagaggat
    29101 gtccgtttga tgcgtgaaac tgtaggacca gaaatgggtg tcaaagcttc cggcggtatc
    29161 cataacgaag aagaagcaat ggcaatgatc gaagctggtg ctacaagaat cggaacaagc
    29221 gcaggagtag caatcgtatc tggttcaact ggtgaaggct attaattttt tcaaaggaga
    29281 agctcatggc agaactcaaa caagaatgga tcaacacagc tattgaagcg ttagataaag
    29341 cctatgttcc ttattcccat tttccggtag gcgcttgtct cgtaacggaa tcaggcaaga
    29401 tttatcaagg catcaacatt gagaatgcat catttggatt aacgaattgt gccgagcgca
    29461 cagctttttt caaagcagta tctgaaggag aaaaaacatt tacgcatcta gtcgtagcgg
    29521 ggcacacccc cgatcctatc tctccttgtg gtgcatgtcg acaagtaatg gcagaattct
    29581 gttcccctga tatgccggtt acgctagtag gagacaatgg tgtgacgaag gcaacgaccg
    29641 ttcgtgcact gctgccttat gcatttactg aaaaagatct ataactcatg ttcattcagt
    29701 cagctgactg ttgaaaaata aaacaattat tttaggaggc tttacagaaa tgaaaaaagc
    29761 aaaattattt ggtttaggtg ctgtcgcact agcagcaggg ttatttttag gtgcatgcgg
    29821 caacaacggc tctactgatt catccggtgg caaaagctca tcagacacaa caactgcagc
    29881 attgatcaca gacacaggtg gtgtagacga ccgttcattc aaccagtcag cttgggaagg
    29941 actagaaaaa tgggggaaag atcaaggact ttcacgagga aatgatggct tccaatattt
    30001 ccaatcttca aatgaatcag attacatccc aaatattgac caagcattga atgctggatt
    30061 caaaacgatt tttggtattg gttataaact aaaaccagcg atccaagaac aagcaacaaa
    30121 caatacaggt acgaactttg ttattatcga tgatgtcatc gatggattag ataacgttgt
    30181 atctgcaact ttcaaagaca acgaagcttc ttatcttgca ggtgttgcag cagcttatac
    30241 gactgaaaca aacgtagtcg gctttatcgg cggtgtaaaa ggtgaagtaa tcgatcgttt
    30301 tgatgctgga ttcaaagccg gtgttgatgc aggtgcgaaa gaattaggaa aagaaatcaa
    30361 agtattgaat caatatgctg gtgacttctc tgctccagat aaaggacgtt caattgctca
    30421 aggtatgtat gcacaaaacg cagatatcat cttccatgct tcaggcggta caggtaacgg
    30481 tgtattccaa gaagcaaaat cattgaacga atcaggcgac aaaaaagttt gggttatcgg
    30541 tgttgaccgc gaccaatcag acgaaggcga atacacatta aacggcgaaa agaaaaactt
    30601 cacattgaca tctacactga aagcagttgg aacagtagta gaagatttgg cacaaaaatc
    30661 agcggacggt aaattccctg gcggcgaaca cactgtttat ggattgaaag aagacggtgt
    30721 tggtctaaca gaaggacaac tttctgacga agcgaaaaaa gcagttgatg aagcaaaaga
    30781 aaaaatcatt tcaggtgacg taaaagttcc agaaactcct gaagaaaact aaaaccattt
    30841 ctatagcctt gttagacaca tgtttgacaa ggctatggtt tgattagaac aagaattgtt
    30901 catttatcca agctgccttg tgacagtttg gataagggaa caactccctg aagtaatgtt
    30961 tgtttcaaaa gaagaacgct cactaagcgt gaatcttagt caatcgttgc cctagataga
    31021 aatcgttagg atgtgaaaaa aaggtggttc aagaaaatat tgtcatagaa atgcagaata
    31081 ttaccaaaga atttggaaca ttcaaggcaa atgacaacat caatttacaa gtaaaggcag
    31141 gagaaattca tgcgttgctt ggagaaaacg gtgctggcaa atctacattg atgaacgtgc
    31201 tttccggatt attagagccg acatcaggga aaattttgat gcgtgggaaa gaagtaaaga
    31261 tcacaagtcc gaccaaagcc aaccaattag ggattgggat ggtccatcag cactttatgc
    31321 ttgttgatgc ctttactgta acagaaaaca tcgtgttggg aagcgagcct agtcgcgcag
    31381 ggatgcttga ccataaaaaa gcacgaaaag agatccaaaa agtttctgaa cagtatggat
    31441 tgtcagtcaa cccagatgct tacgttcgtg atatttcagt tggaatggaa caacgggtag
    31501 aaattttaaa aacactttac cgaggagcag atgtactgat ttttgatgag ccgacagctg
    31561 tattgacccc tcaagaaatt gatgaattaa tcgtgatcat gaaggaatta gtcaaagaag
    31621 gcaagtcgat cattttgatt acgcataagt tagatgaaat caaagcagta gctgaccgct
    31681 gtacagttat ccgccgtgga aaaggaatcg gtacagtcaa cgttaaagac gtaacctcac
    31741 aacaattagc cgatatgatg gttggaagaa cggtttcatt caaaacgatg aaaaaagaag
    31801 cgaagcctca agaagtcgtt ttgtctattg aaaatctagt ggtgaaagaa aatcgtggat
    31861 tagaagccgt gaaaaacttg aacttagagg ttcgtgctgg cgaagtactt ggtatcgctg
    31921 gaatcgatgg aaacgggcag tcggagttga tccaagcttt gactggtttg cgaaaggcag
    31981 aaagcggaca tatcaagcta aaaggggaag acatcactaa taaaaaaccc cgaaaaatca
    32041 ctgaacatgg tgtaggacac gtgccagaag accgtcataa atacgggtta gtcctagata
    32101 tgacattatc tgaaaatatc gcactacaaa cctatcatca aaaaccttac agtaaaaacg
    32161 gcatgctgaa ttattcagta atgaatgaac atgccagaga attgatcgaa gaatatgatg
    32221 ttcgaacaac gaatgaactt gttcccgcaa aagctttatc aggcggaaat cagcaaaaag
    32281 caattatcgc tcgtgaagtc gaccgagatc ctgatctgtt gattgttgca aatccaactc
    32341 gtggactgga tgtaggtgcg atcgaattta ttcataaacg tctgatcgaa caaagggaca
    32401 aatacaaagc agtgttactg atcagtttcg aattagaaga aattttaaat gtttcggatc
    32461 gtattgctgt tatccatgaa ggagaaattg tcggtatcgt tgatccgaaa gaaacatctg
    32521 aaaatgaatt aggtctattg atggcgggct ataccttaga agaagcacga aaagagttag
    32581 aaaagggggc tggcgcacat gaatgatcgc agcgaaaaat tccgtaattt attagtgcct
    32641 gtactttcag ttgttttagg attgatctta ggcgcgatat tgatggctgc ttttggtttt
    32701 cagcccatcc aaggttattc ttctatgcta gatgcagccc ttggttcgca aagagggata
    32761 ggtgaaacgt taaggcaagc aacgccgttg atttttacag cattaggttt ttctgtagct
    32821 aactcagctg ggtttttcaa tattgggctt tctggacaag cactttgcgg gtggatcgtc
    32881 agtgtctggg tggcacttgc gtttccagat ctgccaaaag ttgttttgct tccgttatgc
    32941 gtgatcttag gtgccttagc tggtgcgatg gcagcggctg ttccaggttt acttcgagca
    33001 ttttttggaa caagtgaagt gatcgtaaca atcatgatga actatatctt attatacgta
    33061 agcacatttg ctttacatga agtgatgcct gaaagctatc gctcaagttt ggactcttcc
    33121 aatatgatca gtcaaaatgc ctcactgcgg ttaccatggt taacacaagc ttttggcggc
    33181 tctcgcgtaa actcaggatt atttttagca atcatcgcgc ttattttcgt ttggctgctg
    33241 atgaagaaaa cgactttagg atttgaaatc cgttctgttg gattgaatcc ttttgcttct
    33301 gagtatgcag gaatgagtag taaacgtacg atcgttttgt cgatggtcat ttctggcgct
    33361 ttggcagggc ttggcggtgt ggttgaagga ttaggaacgt atcaaaactt ctttgttcaa
    33421 acgacttcgc tatctattgg atttgacgga atggctgttt ccttgcttgg ttctggatca
    33481 gcaatcggta ttcttttatc agcgatcttg ttcagtattt tgaagatcgg tggacttggc
    33541 atgcagacag gagcaggtgt tccgtttgag atcgtcaatg tatcgattgc tttgattatc
    33601 ttctttgtcg ggatcaactt cctgattcgt ttcgtcatgg ctaaagtctt ccaagggaaa
    33661 aaacaagaag agatcgttgc gacaatagaa tcgaaaccaa cgaatcaaaa ccatgaaggg
    33721 ggcgaactat aatgtcgact atcgaattaa ttgccttgat ccttacttct acactggttt
    33781 attcaacacc gctaattcta acgtcactag gcggaacttt ttctgaacga agcgggatcg
    33841 taaacgtagg gctagaggga atcatggtca tgggtgcttt tagttccatc gttttcaact
    33901 tgacgatgag tccatcactt ggttctatga caccatggat cgctatttta gtgggtggcg
    33961 tcgtaggtat cctattttct ctgattcacg cagtagcaac agtcagtttg cgtgctgacc
    34021 atattatcag cgggacagta attaatctga tggcacctgc tttaggtgtt ttcttgatca
    34081 aagcatggta tggtaaaggt caaactgata acatcacgca gaatctgggg tatttttctt
    34141 tcccaggatt agcagatatt ccggtcattg gaccaatttt ctttaaaaat acattcttac
    34201 ctgcttattg tgctattttg attgctgtcc tagcttgggt gatcatcttc aagacgaaat
    34261 tcggcttgcg cctccgttca gttggtgaaa atcctcaagc tgccgacaca ttaggtatca
    34321 atgtatatgc tatgcgttat tcaggcgtac tgatttctgg cttgttaggc ggtatgggcg
    34381 gtgctgtttt tgctcagtcg atttccggta acttttcagc tggtacgatc gttggacaag
    34441 ggttcatctc catggctgct atgatctttg gtaagtggaa tcctttaggg gcaatgggtg
    34501 cctcattgtt ctttggtttt gctcaaagct tgagtatcat tggcgcacaa ttaccagtta
    34561 tttcttcgat tccgccagta ttgctgcaaa ttgctccata tttgctgaca atcattgtgc
    34621 tggttgtttt cttagggaaa gcttctggtc caaaagcaaa cgggaagaac tatatcaaat
    34681 caaaataatc ttttagcgaa taaaaagggt gggttacggg aagtgttttt cgtctcgccc
    34741 tttttgtttt aaaggcaata aaaataaaaa gaaagaagga ataatcatgt ttaaacgcgt
    34801 acatctgatc gtcatggatt cagtaggaat tggagaagca ccagatgccg ataagttcgg
    34861 tgatgttggt tctgatacat taggacacat tgcgaaagaa gcaggattga acgttccaca
    34921 tttagagcag cttggtttag gaacgatccg tcctcttgaa ggtgtggaat cagtgaaaga
    34981 tcatcaaggc tatgcgacga aattagaaga agtttctgta gggaaagata cgatgacagg
    35041 ccactgggaa atcatgggat tgaatatcaa aactccattt cgtgtgtttc ctaatggatt
    35101 ccctgatgat ctgatcaaac aaatcgaaga attttctggc cgtaaagtcg tatgtaacaa
    35161 accttatagc ggaacagctg ttattgacga ttacggtgaa caccaaatga aaacaggcga
    35221 tttaattgta tatacttctg ccgatcctgt ccttcagatc gcggcacatg aagaaatcat
    35281 tcctttagaa gaattatatc ggatttgcca attcgttcgt gatataacaa aagatgatcc
    35341 gtatatgatt ggccgaatca ttgcacgtcc gtacgtgggt gaaccaggag acttcacacg
    35401 aacaagcaat cgccatgatt atgcgttgaa tccattcgga cacacagtcc ttgattcttt
    35461 gaaagaagca ggaaaggacg tgattgctgt tggaaagatc aacgatattt tcaacggaca
    35521 aggaatcact gaatctgtcc gcacgaaaag caatatggat ggcgttgacc agctgctgaa
    35581 agttatgaaa aacgattttt caggaatcag ctttaccaat ttagtagatt ttgatgcatt
    35641 atacggtcat cgtcgggatg tagtcggcta tgcgcatgcg attgaagaat ttgacttacg
    35701 aattccagaa ttgcttgaag cgatggaaga agacgacttg ctaatgatta cagccgatca
    35761 cggaaacgat ccgacgttta ctggtaccga tcatacgcga gaatatgtac cgttattggt
    35821 ttacagtaaa aaaatgaaag aacaaggggc attgcctcaa ggatattatt cagatatttc
    35881 agcaacgatt gctgagaact ttagtgtttc ttcgactgaa aatggcgaaa gcttcttgaa
    35941 attattaaaa taatcttttt aaataggaga aagcaaatga ccaagttaag cgagatgtta
    36001 caagaaacga cccagtttat ccttgataaa ggagtaaaag aaattgattt tggtttgatt
    36061 ctaggttcag gattaggtga attagcagat gaaatcgaag atgctgtaat gatttcttat
    36121 gatgagatcc cgttctttcc tgtttcgaca gtagttgggc atgcaggaaa gctagtttat
    36181 gggtctctgg caggcaagaa agtgttagcc atgcaaggga gattccacta ctatgaaggt
    36241 cattccatgc aaacagtgac ctatccagta cgtgtgatgg ccggactagg ggcccattca
    36301 gtcattgtga ctaatgcatg tggtggtgtc aacgaatcat ttgtaccagg agatttgatg
    36361 ctgattaccg atcatatcaa ttttactggt gaaaatcctt tgatcggtcc gaatgaagat
    36421 gcaatgggcc ctagatttcc agatatgagt caagcttata cgcctgacta tcaagcaaca
    36481 gcaaaagcag cagcagaaaa actaggtttg gatcttaaag aaggcgtata tatgggctat
    36541 tctggaccaa cttatgaaac gcctgcggag atccgaatgt ctcggacaat aggagccgat
    36601 gcagtaggga tgtcgactgt tccagaagtc atcgtagcag ctcacagcgg attaaaagtg
    36661 ttaggaatct cttgtattac caacttagca gcaggtatgc aagcgaatct gagccatgaa
    36721 gaagttgtag aaacaactca gagagtcaaa caatctttta aagcattggt taaagaaaca
    36781 ttgacattat tgtaatagga ggaacaataa aatgagtgtg catattgaag cgaaagaagg
    36841 cgagatcgcc gataagattt tacttccggg agatcctttg cgagctaaat atattgcgga
    36901 gacttttttg gaagatccag tttgttacaa tcaggtacgc ggtatgttag gctttaccgg
    36961 caaatataaa ggagaacgta tttctgttca aggaacagga atgggaatgc catcagccac
    37021 catctatgcc catgaattga tccagtcgta tggtgtaaag aaattgattc gagtaggtac
    37081 atgcggtgca ttgtcaaaag acgtccatgt tcgtgattta gtactagcac aaggtgcagc
    37141 gaccagttct tcgatgatcg aaaaaaattt ccaagccttt cattttcctc caatcagtga
    37201 tttcaatcta ttactgaaag catatgagat agctaaagaa aagggctata cggttcatgt
    37261 aggaaatgtt ctttcggaag attctttcta caaagatgat ttgacagaga cgttccaatt
    37321 agctgaactt ggcgttttag gagtggaaat ggaagcagcg gctctttatt acttaggcgc
    37381 gaaataccat gtgcagacat taagtttgat gaccgttagc gaccatttga tcaccggtga
    37441 agaaacaaca gctgcagaac gtcaatctac gttcaatgaa atgatcgaag taggattgga
    37501 aacagcgata gcagaataag aaggaaatga aaaagctgcc ctgcattaag taataagaac
    37561 atccaagcaa aaatagctcc tcatgttttt gacttggctg gacttattat tgcaaatgta
    37621 agcttttgaa caccgtttac caagaggttg tgaatctgcc gtttaactct gagtcacaag
    37681 gaacaatttc aaaaaacagc ttttcatgtt tgatgaatat tgtgacttgt gaccgagaag
    37741 ttggcaggag aaaaccgttt atacgaaaaa agggactgtg acaaatcgtg tcacagtccc
    37801 ttttttatag tgttttttat aggtagtatt ttttagcaac tgttaagtca tcatttaatt
    37861 cataaacaag tggttgacct gttggaattt caagatccat gatgtcttcg tcagagatac
    37921 cttcgatatg tttagctagg gcgcgtaatg agttaccatg tgcagctact aaaacagttt
    37981 tgttgtctag taatgctgga gcaatttcat cttgccagaa tggcaaagcg cgttctaatg
    38041 tcactttcaa gttttctcca ccaggaatat cgcgtttgtc taacattgcg taacgacggt
    38101 catttgctgc agaaccttcg tcagtttctt ccattaatgg aggaagagta tcgtaagaac
    38161 gacgccagat gtgcacttgt tcgtcgccgt atttttcagc tgtttctttt ttgttcaaac
    38221 cttgtaattt accatagtga cgttcgttta agcgccatga tttgatttgt ggtacccaaa
    38281 gttgatctga atattctaag atcaagttac atgttttgat tgcacgagtt aaaacagaag
    38341 tgtaagcaac atcaaattcg attccagctt ctttgatttt gcgtccgcct tcttttgctt
    38401 cttcgattcc ttctggtgct agatttacat cagcccaacc agtaaattgg ttcaatgcat
    38461 tccattcact aagaccgtga cgagaaaaaa ctaattttgg catgtttagc tctcctttta
    38521 agataggttt atatttctga aattttcagt tctgttttat tgtacaattt tttggaagag
    38581 atttccatcg aaaaattaga taagattcta tgagaaaaca agtagagaat ataaaaaaac
    38641 gaacagtgcc cacgcaactg ttcgttttta ggtattagat gcataattgc tcgatttctt
    38701 tttcaggtaa attgatatat ttataacatg ttccaactga aacaccgcat tttgaagaaa
    38761 taaattggat cgtttctttt ttttcataat agagacgtcg gatttttttg atcgtttttg
    38821 catcgatttt tggtcttccg ccgatttttc cttttttacg agcttcatct aatccaacta
    38881 atgtacgttc tttgatcaat gcgcactcca tgtttgctag gttttccatt aattggaaat
    38941 agatttttcc catttcgcgc gtatcgattc cttcatcaag actaatcaag tgcagcccgc
    39001 gatctttgaa taattgcgtg agttctgtta attgccgcgt agatttgcct aatcgatgaa
    39061 gttggcaaat gactagtgca tcgccttgtt gcatgtcagc gatcactgac tccaaatgaa
    39121 cagcagtaga atcattttgt tccacttcat atgtctcatg gaaaatttct tgacaaccaa
    39181 agtcagataa ttgatcaacc tgagttgtta aatcaacgtc atttatggtt gtgcgtgcat
    39241 aaccaataat tttcatggtg attcctttct agcagtgatt ttctaactgg atataacagt
    39301 aaaaatgtag cataaagtac gtcaaaaaga tagttttttc gcaaattttc cgctgtcttt
    39361 ttttattgga tgatcaagca gtttttcttt atttacgacg gattgcgtca agaatctcaa
    39421 cgcctttttc tgagccgaca actactttag aaaccatgtt taggaaaagc ccatgttcta
    39481 ctaccccaac aagttcatct aaataggtag ctaagctttc tggctgttcg atgacttcta
    39541 gatgcagatc aatgatgtaa tgaccaccat ctgtggttaa gacttctccg ttttcgtctg
    39601 tacgcaagac gggttggaat cttttttctt caaaaatatg aagtagctgt tgggagccat
    39661 aagggatcac ttcaactggg agtgggaact tcccaagttt atcgactaat ttagaagaat
    39721 caacgatcca aatcgtttct tttgagtacg tagcgactat tttctcgaaa agcaaagcag
    39781 cgccgccgcc tttgatccct tgaaaatcag cactgatttc atcagcaccg tctattgtca
    39841 aatcgactac ggggacttca tcaatactct ttaacggtat tcctaatttt tccgcttgtt
    39901 cttttgtcgc atttgaagta gtaactcctg tgatacgtaa accttcttca cgcatacggc
    39961 gaccaatctc ttctaccata taataggcgg ttgaaccggt cccgagacct acgatcatcc
    40021 cgtcctttac gaatttcgcg gcttcaatgc cagctaattg ttttaaattc atctttgcgt
    40081 ccaccttttt ttagtttgag catcgtgctt tcctcactat tttagcgtga tttacggtaa
    40141 aaagatacca ttctttttta ttccaataaa gctttttcaa aaaaaattgc atctcttgtt
    40201 gacatgtttt ttcattcgtg atattctaac aaaggtgctt tatctacagg tttctgtgaa
    40261 gaaacgtgct gactgtttat caaagcatat ttgataagga tgcagacatt gcatttattt
    40321 tttatcacaa aaaaagaacc acctggatgt gtggaactag acgcaaatcg tgggaaggag
    40381 gaaacaagga atgcctacaa ttaaccaatt agtacgtaaa cctcgtaaat caaaggtgga
    40441 aaaatctaat tcacctgctt tgaacaaagg atataacagc tttaagaaaa ctcaaactaa
    40501 cgtaaactca cctcaaaaac gtggggtagc aactcgtgtg ggtacaatga cacctaaaaa
    40561 accgaactca gctttacgta aatatgcacg tgttcgtttg tcaaacttaa ttgaagttac
    40621 tgcttatatc ccagggattg gtcataactt acaagaacac agtgttgtat tattacgcgg
    40681 tggtcgtgta aaagacttac caggggtacg ttaccatatc gttcgcggtg ctttagatac
    40741 tgctggtgtt aacgaccgta aacaaagccg ttctaaatac ggaactaaaa aacctaaagc
    40801 ttaattttaa ttattaaaca aatgaaactt gcttctacta tatagaagta attactatga
    40861 acaatatttc ggaaggagga gttacggatg ccacgtaaag gtcctgttgc aaaacgtgat
    40921 gttttaccag atcctattta taactcaaaa ttagtaactc gcttgatcaa ccgtgtaatg
    40981 gttgacggaa aacgcgggat tgcagctaac attatctata atgcctttga cttgatcaaa
    41041 gaatctacag gtaacgatcc attagaagtg tttgaacaag caatgaaaaa cgttcaacct
    41101 gttcttgaag ttaaagcacg tcgtgttggg ggttcaaact accaagtacc agttgaagtt
    41161 cgccctgaac gccgcgttac tctaggtctt cgttgggtag taaactacgc tcgcttgcgc
    41221 ggtgaacaca caatggaaga acgcttagca aaagaaatca tggatgctgc aaacaacaca
    41281 ggtgcttctg ttaaaaaacg tgaagacact cataaaatgg ctgatgccaa ccgtgcgttt
    41341 gctcactatc gctggtaaga ttctctctgt ttggtcggct tttataagtc gacctcagat
    41401 agtgtcctta tttttagcaa ctagatagta aagagaggag aaacaaggag aatgaccaga
    41461 gaattttctt tacaaaacac tcgtaatatc ggtatcatgg cccacatcga tgctggtaaa
    41521 acgactgcta ctgaacgtat cttgtactat actggtaaaa tccataaaat tggtgaaact
    41581 catgaaggag cttcacaaat ggactggatg gaacaagaac aagaacgtgg tattacgatc
    41641 acttctgctg ctactacagc acaatggaag ggtcaccgaa tcaacatcat cgacacacca
    41701 ggacacgtag acttcacagt tgaagttgaa cgttcattgc gtgtacttga tggtgctgta
    41761 actgttcttg atgcacaatc aggtgttgaa cctcaaactg aaacagtttg gcgtcaagca
    41821 acaacttatg gtgtacctcg tatcgtattt gcaaacaaaa tggacaagat cggtgctgac
    41881 ttcttatatt cagtatcaac tttacatgac cgtttgcaag caaacgctca cccaatccaa
    41941 ttaccaattg gtgcggaaga cgacttcaca ggaattatcg accttgtaac aatgaaggct
    42001 gaaatgtata caaacgatct tggaacagag atcgaagaaa cagaaattcc tgaagaatac
    42061 cgcgaattag ctgaagaatg gcgcgaaaaa ttggttgaag ctgtagctga aactgatgaa
    42121 gaacttactt tgaaatactt agaaggtgaa gaaatcactg aagctgagtt aaaagaaggt
    42181 atccgccgcg caacagttaa cgtagaattc tacccagtac tatgtggttc agcatttaag
    42241 aacaaagggg ttcaattact attggacgct gttcttgatt acttgccatc accgttagat
    42301 atcccagcta tcaaagggat tgaccctaaa actgatgaag aagtagaacg tcctgcagac
    42361 gacagcgctc ctttctcagc tttagcattt aaagttatga ctgacccatt cgtaggtcgt
    42421 ttaactttct tccgtgtgta ctctggtgtt ttacaatcag gttcatacgt acaaaacgca
    42481 acaaaaggca aacgtgaacg tgttggtcgt atcctacaga tgcatgccaa ctctcgttca
    42541 gaaatctctg aagtttacgc tggagatatc gctgctgctg ttggtttgaa agacacaact
    42601 actggggaca cactatgtga tgaaaagagt ctagttattc ttgaatccat ggaattccca
    42661 gaaccagtta tccaagtcgc tattgaacct aaatcaaaag ctgaccaaga taaaatgggt
    42721 gtggctttac aaaaactatc tgaagaagat cctactttcc gtgctgaaac aaacgtggaa
    42781 acaggcgaaa caatcatcgc tggtatgggt gaacttcact tagatatcat cgttgaccgt
    42841 atgcgtcgtg aattcaaagt tgaagctaac gtaggtgcgc ctcaagtatc ttaccgtgaa
    42901 actttccgtg ctggcactca agccgaaggt aagtttgtac gtcagtctgg tggtaaaggt
    42961 caatacggtc acgtatggat tgaatttaca ccaaatgaag aaggtgctgg tttcgaattt
    43021 gaaaatgcga tcgtcggtgg ggttgttcct cgtgaataca ttccagctgt tgaaactggt
    43081 ttgaaagatg ctatggaaaa tggtgtctta gctggctatc cattagtaga tatcaaagct
    43141 aaactttacg atggttcata ccatgatgtc gattcaaatg aaactgcctt ccgtgttgct
    43201 gcttctatgg cattacgtgc agcagctaag aaagctaatc cagtaatcct tgaaccaatc
    43261 atggctgtag aagttgttat ccctgaagat tatttaggtg atgttatggg tcacgtaact
    43321 gctcgtcgtg gacgtgttga aggtatggaa gctcgcgctg gcggccaaca agttgttcgt
    43381 gcgatggttc cacttgctga aatgtttggt tatgctacaa ctcttcgttc tgctactcaa
    43441 ggtcgtggta catttacaat gacatttgac cactacgaag atgtaccaaa atcagtacaa
    43501 gaagaaatca ttaagaaaaa cggcggaaaa gctgaataat ttcttgaaac cggatagtcc
    43561 tgcaaagaga ttatccaaag agactatatt tttaatatag tttcgtgtaa aataattatc
    43621 gatgatatgg gcatgtggtg taccttgtgt acatcatata cctatcagac aaataaaaaa
    43681 tatattgtat ttttaggagg acattttaaa atggcaaaag aaaaatttga ccgttctaaa
    43741 ccacacgtta acattggtac tatcggacac gttgaccatg gtaaaactac attaacagct
    43801 gcaatcacaa ctgtactatc taagaaaaac ggcggccaag ctatggctta cgatcaaatc
    43861 gatggtgctc cagaagaacg cgaacgcgga atcacaatct caacagctca cgttgagtat
    43921 gaaacagata cacgccacta cgctcacgtt gactgcccag gacacgcgga ttacgttaaa
    43981 aacatgatca ctggtgctgc tcaaatggac ggagctatct tggtagtttc tgctgctgac
    44041 ggcccaatgc ctcaaactcg tgaacacatc ctattgtctc gtcaagttgg tgttccttac
    44101 atcgttgtat tcttgaacaa agtagacatg gttgatgacg aagaattact agaattagtt
    44161 gaaatggaag ttcgtgacct attaacagaa tacgaattcc ctggtgacga tgttcctgta
    44221 gttgctggat cagctttgaa agctctagaa ggcgacgctt catacgaaga aaaaattctt
    44281 gaattaatgg ctgcagttga cgaatacatc ccaactccag aacgtgacaa cgacaaacca
    44341 ttcatgatgc cagttgaaga cgtgttctca attactggac gtggtactgt tgctacaggt
    44401 cgtgttgaac gtggacaagt tcgcgttggt gacgaagttg aagttgttgg tattgctgaa
    44461 gaaacttcaa aaacaacagt tactggtgtt gaaatgttcc gtaaattgtt agactacgct
    44521 gaagctggag acaacattgg tgctttacta cgtggtgttg cacgtgaaga catccaacgt
    44581 ggacaagttt tagctaaacc aggtacaatc acacctcata caaaattctc tgcagaagta
    44641 tacgtgttga caaaagaaga aggtggacgt catactccat tcttcactaa ctaccgtcca
    44701 caattctact tccgtacaac tgacgtaaca ggtgttgttg aattaccaga aggaactgaa
    44761 atggttatgc ctggcgacaa cgtaacaatg gaagttgaat taatccaccc aatcgctatc
    44821 gaaaacggta ctaaattctc aatccgtgaa ggcggacgta cagttggtgc cggtgtcgtt
    44881 acagaaatca aagcttaatt cacttaagtt ttaaagtaaa gcaacattta ttcggacgta
    44941 atcgctcgga acgaaggtct taggatcttt gttccgagct tttttattta ttgcaggatc
    45001 aggaaaaaag acaggagtat tataaataga agaaacacga aataaaagag tcagtatttt
    45061 ctaaatgaaa ctttatgaaa acaaaatgtt ttttacaaga aaaagccttg ctttgtgagt
    45121 ttatatatat tataatgaca aaggtgcgtt tactttagtg aattgcacag acgtaatcag
    45181 aatgatttcg tcggctaaga aacgagaggt tgcgacacgc ccggacgctt tgccacgaac
    45241 gagcgtgacg gaaattttcg tggagctatg tctacttttc aaaataggcg aaggagggaa
    45301 caagatggca aaacaaaaaa ttcgtatccg tttaaaagcg tatgaacacc gtattttaga
    45361 tcaatcagcg gataaaatcg tggaaactgc aaaaagaact ggagctagcg tgtctggtcc
    45421 aattccatta ccaactgaac gcagtcttta cacaatcatc cgtgcgactc ataaatacaa
    45481 agattcacgc gaacaattcg aaatgcgtac acacaaacgt cttatcgaca ttgtgaaccc
    45541 aacaccaaaa actgttgatg ctttgatgaa gctagactta ccatctggtg taaacatcga
    45601 aatcaaatta taaaactaaa acaaaaataa tggaggtgta ctcatgacca aaggaatctt
    45661 agggaaaaaa gtgggaatga cacaaatctt cactgaatct ggtgaattaa ttccagtaac
    45721 tgttgtagaa gctactccaa acgtagtact acaagtgaaa acaatggaaa cggatggcta
    45781 cgaagctatt caagttggtt accaagatat gcgtgaagtt ttatcaaaca aacctgcgaa
    45841 aggtcatgtt gcaaaagcaa acacggctcc taagcgcttc attcgtgaat tcaaaaatgt
    45901 tgagctagga gaatatgaag taggaaaaga aattacagta gatgttttcc aagcaggaga
    45961 cattattgat gtaacaggta ctacgaaagg taaaggattc caaggggtta tcaaacgcca
    46021 cggacaaagc cgcggaccaa tggctcacgg ttctcgttac catcgtcgtc ctgggtcaat
    46081 gggtccagtt gcacctaacc gtgtatttaa aaacaaacgt cttgctggcc gtatgggtgg
    46141 caaccgcgta actatccaaa acttggaagt tgtacgtgtc gatgcagaaa gaaatgttat
    46201 cttgatcaaa ggtaatgttc ctggagctaa aaaatcttta attactatca aatcagctgt
    46261 gaaagctaaa taattgtagg aagaaaggag gaactaagga atgccgaatg tagcattatt
    46321 caaacaagat ggaagccaaa acggtgaaat cacacttaac gaagaaatct tcggaattga
    46381 acctaatgaa agtgttgttt acgatgcaat cgtaatgcaa cgtgcgtcat taagacaagg
    46441 aacacacgcg gttaaaaacc gtagcgctgt tcgcggcggc ggacgtaaac catggcgtca
    46501 aaaaggaaca ggtcgtgcac gtcaaggatc tatccgctca ccacaatggc gtggaggtgg
    46561 cgtagtcttt ggaccaacac cacgttctta cagctacaaa cttcctaaaa aagttcgtcg
    46621 tttagcaatg aaatctgtat tgtcagaaaa agttgctgaa aacaacttgg tagcaatcga
    46681 aggcttgaac ttcgacgcac caaaaacaaa agaattcaaa caagttcttg ctaacttatc
    46741 tatcgactct aaagtattgg ttgttctaga aaaaggtaac gactttgctg ctttatcagc
    46801 acgtaactta ccaaacgttt ctgtagtaac ttctgataat gtgagtgtgc tagacgttgt
    46861 atcaaataca aaagtgttag caacacaaac tgctcttact caaattgagg aggtgcttgc
    46921 ataatgaact tactagacgt aatcaaacgc ccagtgatca ctgaaaaatc aatgcttgcc
    46981 atggatgaca agaaatacac ttttgaagtg gacactcgcg caaacaaaac tttagtaaaa
    47041 caagcggtag aagctgcttt tgacgttaaa gtcaaaaacg tgaacatcgt gaacgtacgt
    47101 gcgaaattca aacgtatggg caaatatgca ggatatacaa aaaaacgtcg caaagcgatc
    47161 gttacattga ctgaagattc aaaagaaatc caattattcg aagctgctga ataagcacta
    47221 atagataact cattaaatta ggagggaaaa acgtggcgat taaaaagtac aaacctacct
    47281 caaatggtcg tcgtaacatg acaggttctg attttgctga aatcacaaca tcaacacctg
    47341 aaaaatcatt gttacaacca ttgaaaaacc atgctggacg taacaacaac ggccgcatca
    47401 ctgtacgtca tcaaggtggc ggacacaaac gccaatatcg cgtgatcgac ttcaaacgta
    47461 acaaagataa cgttgttgca actgttcaaa cgatcgaata tgatccaaac cgttcagcta
    47521 acatcgcgtt agtacactat gaagatggag tgaaagctta catcttagca cctaaaggac
    47581 tagaagtggg tatgaaactt gtttcaggtc cagaagcaga tatcaaagtc ggaaatgctc
    47641 ttccattaga aaacattcca gttggtactg ttatccacaa cattgaaatg aaacctggta
    47701 aaggtggaca attgatccgt tctgctggta caagtgctca agtacttggt aaagaaggca
    47761 aatacgtatt gatccgcttg aactctggtg aagttcgtat gatcttagct gcatgccgtg
    47821 ctacaatcgg ttctgtaggt aacgaacaac acgaattaat caacatcggt aaagctggac
    47881 gctctcgttg gatgcgtaaa cgcccaactg tacgtggtag cgtaatgaac cctaatgatc
    47941 acccacacgg tggtggtgaa ggtaaagctc ctatcggacg taaagcacca gtttctcctt
    48001 ggggtcaacc agctcttggt tacaaaactc gtaacaaaaa agctaaatca gacaaactta
    48061 ttgttcgtcg tcgcaaaaat aaataattac tttgaccatg tgtcgcacat tttgagagga
    48121 ggttcaccat gggtcgtagt ttaaagaaag gaccttttgt cgatgagcat ttaatgaaaa
    48181 aagttgaagc tcaagcaggg gttgaaaaga aaaaagttat caaaacttgg tcacgccgtt
    48241 caacagtttt cccaagtttt gttggatata ccatcgcagt ttatgatgga cgtaaacatg
    48301 taccagttta catccaagaa gacatggtag gacacaaatt aggtgaattt gcacctacaa
    48361 gaacttatcg tggacatgct gctgacgata agaaaacgaa acgctaattt gaggggagga
    48421 taaatccaaa tggcagaaca aatcacatca gctaaagcaa ctgcaaaaac agttcgcact
    48481 tcacctcgaa aagcccgttt agtaattgac cttatcagag gtaaaagtgt cgcagatgca
    48541 atttcaatct tgaaattcac accaaacaaa tctgctggaa tcattgaaaa agtattgatg
    48601 tcagcagttg cgaatgcaga aaataacttt gacttagatg ttgaaaactt ggtagtaagc
    48661 gaagcttttg ttaacgaagg acctacaatg aaacgtttcc gtcctcgtgc aaaaggatca
    48721 gcttctccaa tcaacaaacg tacaagtcat atcacggtag tcgtatcaga aaaataagga
    48781 gggataatca gtgggtcaaa aagtacatcc aattggaatg cgtgtaggca tcatccgcga
    48841 ctgggatgct aaatggtatg ctgaaaaaga gtatgctgag ttcttacacg aagatttaag
    48901 aatccgtaaa tttatttcaa ctaaacttgc tgatgctgct gtatcaacag ttgaaatcga
    48961 acgcgcaaca ggtcgcgtaa acatttcaat tcacacagct aaaccaggta tggttattgg
    49021 taaaggcggt tctgaagtcg aaagcctaag aaaagaatta aacaaattaa ctggtaaaag
    49081 aatccatatc aatatcgtgg aaatcaaaaa accagatcta gatgcaaaat tagtaggtga
    49141 aggaatcgca cgtcaattag aaaaccgtgt tgctttccgt cgcgctcaaa aacaagccat
    49201 ccaacgctca atgcgtgctg gcgctcaagg gatcaaaact caagtatctg gtcgtctaaa
    49261 cggagcagat atcgctcgtt cagaaggata ctcagaagga actgttcctc ttcatacttt
    49321 acgtgcggac attgattacg catgggaaga agcagataca acatacggaa aactaggagt
    49381 taaagtgtgg atctatcgtg gagaaattct tccaactaaa aaaaacactg agaaaggagg
    49441 gaaataatca tgttagtacc taaacgtgta aaacaccgtc gtgaattccg cggaaaaatg
    49501 cgcggggaag ctaaaggcgg aaaagaagta gcattcggtg aatacggttt gcaagctgtt
    49561 gattcacatt ggatcacaaa ccgccaaatc gaagctgctc gtatcgcaat gactcgttac
    49621 atgaaacgtg gtgggaaagt atggattaaa attttccctc acaaatctta tactgccaaa
    49681 gcaattgggg tacgtatggg ttctggtaaa ggggcacctg aaggatgggt tgcaccagta
    49741 aaacgtggta aaatcatgtt tgaaatcgca ggcgttcctg aagaagtagc tcgtgaagcg
    49801 ttacgtctag cttctcacaa attaccaatg aaaactaaga tcgtaaaacg tgaggaaatg
    49861 ggtggtgaat cgaatgaagg ttaaagaaat cagagaatta accactgccg aaatgcttga
    49921 tcaagaaaaa caattaaaag aagaattgtt taatcttaga ttccaattag caacaggtca
    49981 attagaaaac actgcacgta ttaaagaagt acgtaaatcg attgcacgca tcaaaactgt
    50041 gttgcgtgaa caagctaagt aattatggaa ggaggccatt aagcgtatga ctgaaggaag
    50101 aaatcaacgc aaagtttatc aaggtcgcgt ggtttcagac aaaatggata aaacaattac
    50161 cgttgctgtc gaaacaaaga aaaaccaccc tatctatggc aaacgcatga actattctaa
    50221 gaaatacaaa gtgcatgatg aaaacaacac agcaaaagtt ggcgacatcg tgagaattat
    50281 ggaaactcgt ccattatcag ctacaaaacg tttccgtttg ttggaagtag tcgaagaagc
    50341 agttattatc taataacacg agattttcct atatagattg aatgcaaaat ctgaaaggag
    50401 gatacacaac gtgatccaag cagaaagtcg tttaaaagtc gctgataact caggcgcacg
    50461 tgaaattttg acgatcaaag tccttggtgg atctggtcgt aaaactgcta acatcggtga
    50521 tgtgatcgtt gctacggtta aacaagcaac gccaggtggg gttgtcaaaa aaggtgacgt
    50581 cgttaaagcc gttatcgttc gaacaaaatc aggagctcgt cgtacagacg gttcatatat
    50641 taaatttgat gaaaatgctg cagtgattat tcgtgatgat aaaagccctc gtggaacacg
    50701 tatctttggg ccagttgcac gtgaactacg tgaaaacaac ttcatgaaga tcgtttcact
    50761 agcaccagaa gttttataat cttgagacac ttatcaaagg aggtgcgaaa cagttatgtt
    50821 tgttaaaaaa ggcgataaag ttaaagttat cactggaaaa gacaaaaaca aagaaggcgt
    50881 tgtactagca gcgttcccga aaaaagataa agttattgtc gaaggtgtaa atgtcgttaa
    50941 aaaacaccaa aaaccttctc aagctgctcc tcaaggtgga attgttgaga tggaagcgcc
    51001 aattcatgtt tctaacgtga tggtggttga ctctactggt gtagctggcc gagttggcta
    51061 caaagaagta gacggcaaaa aagtccgtgt ttctaaaaaa accggtgaag ttttagataa
    51121 ataaaagttt aaggaaggag ggtcttactg aatgaaccgc ctaaaagaaa aatatcttaa
    51181 agaagtaact ccatcattga tggaaaaatt taactatagc tctgttatgc aaacacctaa
    51241 agttgaaaaa atcgtcatca acatgggtgt tggtgatgcg gtatcaaacg ctaaaaactt
    51301 agataaagca gttgaagaat taactttgat cactggtcaa aaaccaatga ttacaaaagc
    51361 taaaaaatca atcgctggat tccgtttacg tgaaggaatg ccaattggtg caaaagttac
    51421 attgcgcgga gaaagaatgt acgaattctt agacaaattg gtttctgttt cactaccacg
    51481 tgtacgtgac ttccatggtg taagtaaaaa agcctttgac ggtcgtggaa actatacttt
    51541 aggtattaaa gaacaattga tcttcccaga agtagattat gatttagtag ataaagtacg
    51601 tggtatggac atcgttattg taacaacagc aaactctgat gaagaatctc gtgagttgtt
    51661 gacacaatta ggcatgccat tccaaaaata attaaaggag gcgaactacg tggctaaaaa
    51721 atcaatgatt gctaaaaaca aacgtcctgc aaaattttca acacaagctt atactcgttg
    51781 cgaacgttgc ggacgtccac attcagttta tcgtaagttc catctttgcc gtatttgctt
    51841 ccgcgaactt gcctataaag gtcaaattcc cggcgtgaag aaagctagct ggtaaaaagt
    51901 aaattcgcat aagaaggagg taaaagatca atggtcatga cagatccaat tgcagatttt
    51961 ctaactcgta tccgtaacgc aaacatggtt aaacatgagt ctttagaagt acctgcatca
    52021 aaaattaaac gtgacatcgc tgaaatcttg aaacgtgaag gtttcgttcg tgatgttgaa
    52081 tacatcgaag atgacaaaca aggcgtgatc cgtgttttcc taaaatttgg taaaaacggc
    52141 gaacgtgtta tcacaaactt aaaacgtatc tctaaaccag gtttgcgtgc ttatgtcaaa
    52201 gctgacgaag taccaaaagt attgaacggt ttaggtatcg caatcatttc gacttctgaa
    52261 ggtgttgtca ctgataaaga agctcgtgaa aaaaatatcg gcggcgaagt tatcgcttac
    52321 gtatggtaat caagtagaat ctacaaggag gtgtctttaa gtgagccgta tcggaaataa
    52381 agtcgttgtt atccctgaag gagtaacagt tactcaagac ggaaacaacg ttacagttaa
    52441 aggaccaaaa ggtgaattga ctcgtacttt ttcagcagat atcaaaatga atatcgaagg
    52501 aaacgaagta actttcactc gtccaaacga cagtaaagaa atgaaaacaa ttcatggaac
    52561 aactcgtgcg aacttcaaca acatggttgt aggtgtcagc gaaggtttcc aaaaagcgtt
    52621 agaactaatc ggggttgggt accgtgcaca attacaagga tcaaaacttg tattgaacgt
    52681 tggttactca catccagtag aaatcacacc accagctgga gtaactgttg aagtaccagc
    52741 taacacacaa gtaattgtta aaggtgcgaa caaagaagaa gttggcgaat tagctgcaaa
    52801 cattcgtgga actcgtcctc cagaacctta taaaggtaaa ggtattcgtt atgttggcga
    52861 atatgtacgc cgtaaagaag gtaaaactgg taaataatcg tttagaaaaa ttgcatagaa
    52921 gccagcagca ggaaaaaggc atttggctga agtttacctt caaaaaaata catttttact
    52981 tgtgccgcag cttctatttt tcgaaacgta tttactgctt gaaatagtag tacaataaaa
    53041 ataaagaggt gacaattgtg attacaaaac cagataagaa caaaacacgt caaaagagac
    53101 atcgtcgtgt acgtaacaat atctctggta ctgctgagcg cccacgcttg aacgtttttc
    53161 gttctaacaa aaacatctac gctcaaatta ttgatgacgt agcgggtgta acgctagcaa
    53221 gtgcctctac cttggataaa gaaatttcag gtggaacaaa aacagaagca gcacaagctg
    53281 tcggtaaact agttgctgaa cgtgcagctg aaaaaggcat taaagtagta gtctttgacc
    53341 gtggtggata cctttaccat ggccgtgtgc aagctttagc tgaagctgct cgcgaaaatg
    53401 gactagaatt ttagaagaag gaggaatacc attcatggtt tatatcgatc caaaacattt
    53461 ggaattagaa gaccgcgttg ttgcaattaa ccgtgtaaca aaagttgtta aaggtggacg
    53521 tcgtctacgt tttgctgctt tagttgttgt cggtgacaaa aacggacacg taggatttgg
    53581 tacaggtaaa gctcaagaag ttcctgaagc aatccgtaaa gcaattgaag atgcgaagaa
    53641 aaacttagtt gaagtaccta tggttggttc aacaatccca cacgaagtaa tcggtgcttt
    53701 tagtggtggt agaatcctaa tgaaacctgc tgtagaaggt tctggggttg ctgctggtgg
    53761 accagttcgt gccgtattag aattagctgg ggtagcagat atcacttcta aatctttagg
    53821 ctcaaataca ccaatcaacg ttgttcgcgc aacagttgaa ggtttgaaac aattgaaacg
    53881 tgcagaagaa gttgcagaac ttcgcggcaa atcagttgaa gaattaatcg gataaggagg
    53941 acaacaataa tggctgaatt aaaagttact ttaaaacgca gtattatcgg acgtcctcaa
    54001 aaccaacgcg atacagttaa agcgttaggt cttacaaaga ttaacagttc agtggttaag
    54061 ccttctaatg aagcaattaa aggcatgatc aatactgttt ctcacctagt ggacgttgaa
    54121 gaaatttaat ttagaaaaat cataactaga acattaagga ggtgccaaac ctatgaaact
    54181 tcatgaatta aaacctgctg aaggttcacg ccaagtacgt aaccgtgttg gtcgtggtac
    54241 ttcatctggt aacggaaaaa ctgccggacg cggacaaaaa ggacaaaaag ctcgttcagg
    54301 cggcggtgtg cgtttaggat ttgaaggggg acaaacacca ttgttccgtc gtttaccaaa
    54361 acgaggattc acaaacgtga accgtaaaga ctatgcagtc attaatcttg atgtattgaa
    54421 ccgctttgaa gacggagctg aagtaactcc tgttgcttta gttgaagctg gaatcgtgaa
    54481 aaacgaaaaa gctggaatca aagtattagc taatggggaa ctaaacaaga aattaactgt
    54541 gaaagcagca aaattctcta aagcagctca agaagcaatt gaagcggctg gtggatcaat
    54601 cgaggtgatc taatgtttag actattaaaa gatgctttta aagtaaaaga cattagatcg
    54661 aaaatcctat ttactgtatt tgtgttgttt gtatttcgcc taggtgcaca tattactgta
    54721 ccaggcgtaa atgctgcttc attgcaaaac ctttcttctt tgccattttt caacatgttg
    54781 aacttggtaa gtggtagtgc gatgcaaaac ttttccattt tctcaatggg ggtttcacca
    54841 tacattactg cttctatcgt cattcagctt cttcaaatgg acatcgtgcc tcgatttgtt
    54901 gaatggtcga aacaagggga agttggtcgt aaaaaactga atcaagcgac tcgatatctg
    54961 acattagtat tggcatttat ccaatctgtc ggtatcactg ctggattcaa tcaatacgct
    55021 caattaggtt tcgtgaataa tcctaacgta gcaacttacg taacgatcgg tttgattcta
    55081 acagccggta cgatgtttgt tacttggcta ggagaacaga tcacagaaaa aggtatcgga
    55141 aatggcgtat caatgatcat ttttgcaggt atcatttcta gattgcctga aagtatcaga
    55201 gaaatttatg aagattactt tataaatatt gatccttcag acatatggaa atctgctatt
    55261 tttgtggcca ttttactagt agcggtcttg ttgatcgtga cattcgtaac atttttccaa
    55321 caagctgaac gcaagatccc gatccaatat acgaaacgcg tagctggcgc gcctacaagc
    55381 agctatctgc cgttgaaagt aaatgcagct ggagttattc cagttatctt tgctagttcg
    55441 tttattgcta cgccaaatgc tgttttacaa gcattgagcg gtagattcgg ttctgaaaac
    55501 tggtacaaag tgatgatgga gatctttaac tataatacag cacctggtgc tacgatttat
    55561 acgattctga tcgttgcctt cactttcttc tacgcctttg ttcaagtaaa ccctgagaaa
    55621 ttagcggaaa acttgcaaaa gcaaggaagt tatataccaa gcgtacgccc tggtaagggg
    55681 actgaagagt atgtctcaag attactgatg cgtttaagta cagttggtgc agtcttcctt
    55741 ggtttagtgg cattgcttcc aatcattgct caaatggttt gggatcttcc acaatcaatc
    55801 ggattaggcg gtacaagctt actgatcgtt atcggtgtag ctttagaaac tgcgaaacag
    55861 ttagaaggct taatgctgaa acgtaaatat gttggattca ttgagtaaga aagcgtgttg
    55921 aggtttcccc tcaacacacg ttctcacaca tttattagga gggaaaacaa tgaacctcat
    55981 tttaatgggg ctgccaggcg caggtaaagg aacacaagct gaaaaaatca ttgatgctta
    56041 tggtattccg cacatctcaa caggagatat gttccgcgca gcgatgcaaa acgaaacagc
    56101 tcttggcctg gaagcaaagt cttatatgga taaaggtgca ttagttcctg atgaagtaac
    56161 aaatggaatc gtgaaagagc gactagctga accagataca gagaaaggct tcttattaga
    56221 tggctttcca cgtacgctag atcaagcaaa agctctggac gcaatgctaa aagatttgaa
    56281 taaaaaaatt gatgctgtca tcgatatcca tgttggtgaa gaaatcttgg tcgaacgtct
    56341 agccggccga tttatctgca gcaattgtgg agcaacttac cataaaattt tcaaccctac
    56401 aaaagttgaa gacacatgtg atcgttgcgg tgggcatgaa ttctatcaaa gagaagatga
    56461 taaacctgag acggttaaaa atcgtctggc tatcaacatc aaaaatagtg aaccaatctt
    56521 ggcttattat aaagaacaag gtttgctaaa cactattgat ggtgctcgtg aaatcgatgc
    56581 tgtatttgca gatgtacaaa aaatcattga aaaatagcac gattgcccgt agccgacttg
    56641 cattatgcag ttactcttgt caagttcaat aaattatgat agaatatgtc agtccgactt
    56701 ctagagaatc ctctagatga tccattttga gaattttgag gtgggaactg cagtttccgc
    56761 cattttatcg tgtgaaaatg cgaaacaagt acaaggaggt actgtgcgtg gcaaaagaag
    56821 atatgattga agtcgaaggt acagtcgtcg aaactttgcc gaatgcaatg tttaaagttg
    56881 aactagaaaa cggacaccaa gttcttgcta ctgtttcagg taaaattcgt atgcattaca
    56941 tccgtatctt acctggtgac aaagtaacgg ttgaattgtc tccatacgat cttaaccgtg
    57001 gccgtattac ttatcgcttt aaataattgt actccgtaaa actacaggag gtattatcat
    57061 gaaagtaaga ccatcagtaa aaccaatgtg cgaacattgt aaagtaattc gtcgtaaagg
    57121 acgcgttatg gtgatttgcc cagcaaatcc aaaacataaa caacgtcaag gataattgga
    57181 ggtgctataa atatggctcg tattgcagga gtagatattc ctcgtgataa acgtgtagta
    57241 gtttcactta cttatatcta tggtattggt aacactactg ctaaaaagat cttagctgat
    57301 gctggtgtat ctgaagatgt tcgcgttcgt gaattaacga atgaacaaac agatgctatc
    57361 cgtgcagaag tagataaatt aaaagttgaa ggggatcttc gtcgtgaagt gaacttgaac
    57421 atcaaacgtt tgatggaaat cggttcttac cgtggaatcc gtcatcgccg tggattgcct
    57481 gttcgtggac aaaacacgaa aaacaatgca cgtactcgta aaggcccagc tcgcgctatc
    57541 gctggcaaga aaaaataatt cttaagtgaa ggaggttaaa acttcatggc agcaaaaaaa
    57601 gtgaatcgta aacgccgtgt gaagaagaat atagaagcag gtattgcaca tatccattct
    57661 acattcaata acactatcat catgatcact gatactcacg gaaatgcatt agcgtggtca
    57721 tcagctggtt cattaggttt caaaggaagc aaaaaatcaa ctccttttgc cgctcaaatg
    57781 gcagcagaaa ctgctacaaa agcagcaatg gaacacggat taaagactgt tgaagtaaca
    57841 gttaaaggac ctggttctgg acgtgaagca gcaattcgtt cattacaagc tacaggttta
    57901 gaagtgactg caattcgtga cgtgactcca gttcctcata atggtgcccg ccctccaaaa
    57961 cgccgtcgtg tttaatgagt gccgctcatt ttgagtttta tcagtaacgt cattgaacaa
    58021 gacacttttc gttttgaaag gggtaaagat aagaatgatt gagttcgaaa aaccaagaat
    58081 cgcaaaaatt gatgaagaaa aagattatgg caagttcatc gttgaacctc ttgaaagagg
    58141 gtatgggact actttaggga attccctacg tcgtatttta ttatcttctt tgccaggtgc
    58201 tgccatcact aatattcaaa tcgatggtgt actgcatgaa ttctctacca tcaaaggtgt
    58261 tagagaagac gtcactcaaa tcatcttgaa cattaaaggc ttagcgctga aaatgtatgg
    58321 ccaagaagaa aaaacccttg aaatcgatat taccggacct gctacagtaa ctgctggcga
    58381 tattatcgtt gatagtgatg tagaaattct taacaaagat atgtacatct gtagtgtctc
    58441 tgaaggagct acgttccatg ctcgcttaac agtgaaacct ggacgtggtt atgttcaagc
    58501 agatgaaaat aaaaaagaag atatgccaat tggcgttctt ccagtcgatt caatctacac
    58561 acctgttcgt cgtgtgaact atcaagttga gaacacacgt gttggacacc gtgaagattt
    58621 tgataaattg acgatggaga tttggacaga tggttcaatt gaaccgttgg aagcaatgag
    58681 tttgtctgcg aaaatcatga ctgaacattt ggatattttt gtgaacttaa ctgatgaagc
    58741 aaaaaatgct gaaatcatgg tcgaaaaaga agaaacacaa aaagaaaaaa tgttggaaat
    58801 gacgatcgaa gagttagact tgtctgttcg ctcttacaat tgtttgaaac gtgcaggaat
    58861 caatactgta caagaactta caaataaatc tgagcctgaa atgatcaaag tccgtaactt
    58921 aggacgtaaa tcattagaag aagttaaagc aaaattgcac gacttaggtt taggtttacg
    58981 taaagaagat taaaaccttt tacgaaggag ggaaaccaac gtgagttatc gtaaactagg
    59041 acgcacatct agccaacgta aagcgatgtt gcgtgattta acaactgatt tattaatcaa
    59101 cgaacgtatt gtgactactg aagctcgtgc gaaagaagtt cgctcaactg cagaaaaaat
    59161 gatcacttta ggtaaacgcg gagatttgca tgcacgtcgt caagctgcgg ctttcgtacg
    59221 taatgaagtt gccagcgtgc gtgaagaaaa tgaggacatc gtcattgaat cagctttaca
    59281 aaaactgttc aacgatattg cacctcgtta cgcagaacgt caaggcggat acacacgcat
    59341 tttgaagaca gaaccaagac gcggtgatgg tgcaccaatg gttgtcttag aacttgtcta
    59401 aaatcacact aataacatca ctttatagat gattctttta gagtgttatg atgttggaag
    59461 atatttttcc gagtctagct cagcgctgct cctcaagcga atatcgcttg gggagtaagc
    59521 actcatcata aagtgttttt ttataccttc aattacctgt catgacagat atgtttgaag
    59581 gcataaaaaa acattttaag cgagaaataa ttgaaaattt tgtctttttt tgatagtgtt
    59641 agtaggaact ttaggatgaa gaaggagtaa tcatggagcc aattattgaa ttagaaaaga
    59701 taaattataa ataccagcca gatgatcttc gccccgcatt gaaagatgtc tcctttacaa
    59761 ttgataaagg tgagtggatc gctattattg gacacaatgg ttcaggaaaa tcgacacttg
    59821 ccaaaacgat caatggattg cttctgccag aatcagggat cgtgaaagtg ggaaatcaaa
    59881 tattggatga agaaaatatt tggacgatcc gacagatggt tggaatggtt tttcaaaatc
    59941 cagacaatca gtttgttggt tcgacagtag aagatgacgt tgcttttggt cttgaaaatc
    60001 aagggattcc cagagaagaa atgctagtgc gtgtgaaaga tgcacttgag aaagttagaa
    60061 tggctgagtt tgcttcacgc gagcctgctc gtttatcagg agggcaaaaa caacgtgttg
    60121 ccattgccgg cgtggttgcc ttacgacctg atatcattat tttagatgaa gcaaccagta
    60181 tgctagatcc agaaggtcgt gaagaagtga tctctacgat caagaagatc aaagaagaaa
    60241 gtcaattgac cgttatatct atcactcatg atattgatga agcagctaat gccaatcgga
    60301 tactagtgat gagacaaggc gaacttgtcc gtgaaggtac accaaaagaa attttttctg
    60361 ctggtcctga actgatcgac ttaggtctag atctaccatt tcctgaaaaa ctgaagagcg
    60421 cactgaaaga acgtggggtt gatgtgccca gtgaatatat gacagaagaa aggatggtgg
    60481 attggttatg gacatccgtt ttgaacaagt agacttcact tatcagccga atacaccatt
    60541 tgaacaaaga gccttatttg acatcaatat gacgatcaaa gaaaacagtt atactgcatt
    60601 agtagggcat accggaagcg ggaaatcaac cttacttcag catttgaatg cgcttgttaa
    60661 gccaacaagc ggtacagtcc atattggaga acgagatatt cagccggata cggataataa
    60721 gaatttaaag cccatccgaa aaaaagttgg catcgtcttt cagtttccag aagcacagct
    60781 attcgaagaa acggtagcaa aagacattgc tttcggtcct aaaaactttg gtgtcagcga
    60841 agaagaagca ctagtcctag caaaagaaac attagaacaa gttgggctgg atgaaagcta
    60901 tttggaacgt tcgccatttg aactttcagg agggcaaatg cgtagagtag caatcgctgg
    60961 tgtgcttgcc atgaggccgg aagtacttgt attggatgaa ccaacggcag gacttgatcc
    61021 ccagggcaga aaagaaatga tggagatgtt ttggcgtctt cataaggaac agcagatcac
    61081 tattgtatta gttacgcatt taatggatga tgtagccaat tttgcagatt atgtctatgt
    61141 gttggaaaag ggacgaatcg ttaatagcgg ggagccgcaa gaggtctttc aaaacattga
    61201 atggctaaaa gaaaaacaac taggcgtacc tactgccacc gagtttgcag aagaattaat
    61261 ggctaaaggg atgaactttg ctaccttgcc tttgacagct gaagaattag cagacgctat
    61321 cgtccattat gctggaggaa atgcccatga tgaataaatt gatatttggt cgttatatgc
    61381 ctggagattc cttgatccat cgattggatc cccgagctaa attattagcc agtttttatt
    61441 ttatcggaat cattttttta gcaaataatt gggaaacgta ccttttgttg gcatttttca
    61501 cattactttc tgtcttcttg tcaaaagtag atttgaaatt ttttatccga ggcattcgcc
    61561 cattgatctg gctaatcctc ttcactgtag ctttgcaggt cttgtttaca agtggcggag
    61621 aaacttattg gagttggggt gttttccata tcactgaatt tggtgtgatg aacggcctct
    61681 ttattttctg tcgattcgtg ttgatcattt ttatgtcgac tctactcacg cttactacac
    61741 cgcctttgga attatcggat gcgattgagt acttgcttcg tccattgaaa gccgtacggt
    61801 tcccagtcca cgaaatttca ttgatgctgt cgattgctct tcgttttgtg cctacattga
    61861 tggacgaaac agaaaagatc atgaacgctc agcgcgctcg cggtgttgat tttggtgaag
    61921 gaaacctagt tcagaaaatg aaagcagtca ttcctttgct gattccttta tttgtcagca
    61981 gcttcaaccg tgcggaagat cttgcgactg ctatggaagc aagaggctac aaaggtgggg
    62041 aaggaagaac gaaatatcgt gtattgcatt ggcataatag agacacgatg gttatcattg
    62101 cttttgtatt attaactgtg ctactgatct ttttaagagg ataaacgttc agtgggccgc
    62161 tatctattct tttcttgctg atctgtttgc aagaaaggat gcaagcagtg tatgacaaga
    62221 aatattaggg tctgtggcag actaaggtca caggcctctt tcttttatct gaaaagaaag
    62281 aacgaaaaa
//
LOCUS       DAZKNH010000004        61286 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000004 DAZKNH010000000
VERSION     DAZKNH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.004"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..976)
                     /locus_tag="ACG8MG_000238"
     CDS             complement(<1..976)
                     /locus_tag="ACG8MG_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0374317.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTT"
     gene            complement(1231..1740)
                     /gene="mreD"
                     /locus_tag="ACG8MG_000239"
     CDS             complement(1231..1740)
                     /gene="mreD"
                     /locus_tag="ACG8MG_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFQ0374318.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(1744..2598)
                     /gene="mreC"
                     /locus_tag="ACG8MG_000240"
     CDS             complement(1744..2598)
                     /gene="mreC"
                     /locus_tag="ACG8MG_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFQ0374319.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(2753..3391)
                     /locus_tag="ACG8MG_000241"
     CDS             complement(2753..3391)
                     /locus_tag="ACG8MG_000241"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFQ0374320.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(3392..4042)
                     /gene="rpe"
                     /locus_tag="ACG8MG_000242"
     CDS             complement(3392..4042)
                     /gene="rpe"
                     /locus_tag="ACG8MG_000242"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFQ0374321.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(4055..4954)
                     /gene="rsgA"
                     /locus_tag="ACG8MG_000243"
     CDS             complement(4055..4954)
                     /gene="rsgA"
                     /locus_tag="ACG8MG_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFQ0374322.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(5119..7188)
                     /gene="pknB"
                     /locus_tag="ACG8MG_000244"
     CDS             complement(5119..7188)
                     /gene="pknB"
                     /locus_tag="ACG8MG_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFQ0374323.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(7185..7925)
                     /locus_tag="ACG8MG_000245"
     CDS             complement(7185..7925)
                     /locus_tag="ACG8MG_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFQ0374324.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(7938..9296)
                     /gene="rsmB"
                     /locus_tag="ACG8MG_000246"
     CDS             complement(7938..9296)
                     /gene="rsmB"
                     /locus_tag="ACG8MG_000246"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFQ0374325.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(9296..10234)
                     /gene="fmt"
                     /locus_tag="ACG8MG_000247"
     CDS             complement(9296..10234)
                     /gene="fmt"
                     /locus_tag="ACG8MG_000247"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFQ0374326.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(10227..10718)
                     /gene="def"
                     /locus_tag="ACG8MG_000248"
     CDS             complement(10227..10718)
                     /gene="def"
                     /locus_tag="ACG8MG_000248"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0374327.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(10733..13141)
                     /gene="priA"
                     /locus_tag="ACG8MG_000249"
     CDS             complement(10733..13141)
                     /gene="priA"
                     /locus_tag="ACG8MG_000249"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFQ0374328.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(13394..13699)
                     /gene="rpoZ"
                     /locus_tag="ACG8MG_000250"
     CDS             complement(13394..13699)
                     /gene="rpoZ"
                     /locus_tag="ACG8MG_000250"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0374329.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(13704..14318)
                     /gene="gmk"
                     /locus_tag="ACG8MG_000251"
     CDS             complement(13704..14318)
                     /gene="gmk"
                     /locus_tag="ACG8MG_000251"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0374330.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(14805..15089)
                     /locus_tag="ACG8MG_000252"
     CDS             complement(14805..15089)
                     /locus_tag="ACG8MG_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374331.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(15107..15997)
                     /locus_tag="ACG8MG_000253"
     CDS             complement(15107..15997)
                     /locus_tag="ACG8MG_000253"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFQ0374332.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(16017..16235)
                     /locus_tag="ACG8MG_000254"
     CDS             complement(16017..16235)
                     /locus_tag="ACG8MG_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFQ0374333.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(16205..17905)
                     /locus_tag="ACG8MG_000255"
     CDS             complement(16205..17905)
                     /locus_tag="ACG8MG_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFQ0374334.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            18172..18945
                     /locus_tag="ACG8MG_000256"
     CDS             18172..18945
                     /locus_tag="ACG8MG_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374335.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(19026..19595)
                     /gene="thiT"
                     /locus_tag="ACG8MG_000257"
     CDS             complement(19026..19595)
                     /gene="thiT"
                     /locus_tag="ACG8MG_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFQ0374336.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(19661..19763)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19769..20740)
                     /locus_tag="ACG8MG_000258"
     CDS             complement(19769..20740)
                     /locus_tag="ACG8MG_000258"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0374337.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(20834..22207)
                     /gene="glmU"
                     /locus_tag="ACG8MG_000259"
     CDS             complement(20834..22207)
                     /gene="glmU"
                     /locus_tag="ACG8MG_000259"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFQ0374338.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(22710..23528)
                     /gene="purR"
                     /locus_tag="ACG8MG_000260"
     CDS             complement(22710..23528)
                     /gene="purR"
                     /locus_tag="ACG8MG_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFQ0374339.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(23695..24519)
                     /locus_tag="ACG8MG_000261"
     CDS             complement(23695..24519)
                     /locus_tag="ACG8MG_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFQ0374340.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(24492..25172)
                     /locus_tag="ACG8MG_000262"
     CDS             complement(24492..25172)
                     /locus_tag="ACG8MG_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0374341.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(25195..26133)
                     /locus_tag="ACG8MG_000263"
     CDS             complement(25195..26133)
                     /locus_tag="ACG8MG_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0374342.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(26378..27229)
                     /gene="ispE"
                     /locus_tag="ACG8MG_000264"
     CDS             complement(26378..27229)
                     /gene="ispE"
                     /locus_tag="ACG8MG_000264"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFQ0374343.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(27383..29956)
                     /gene="mprF"
                     /locus_tag="ACG8MG_000265"
     CDS             complement(27383..29956)
                     /gene="mprF"
                     /locus_tag="ACG8MG_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0374344.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            30097..30903
                     /gene="proC"
                     /locus_tag="ACG8MG_000266"
     CDS             30097..30903
                     /gene="proC"
                     /locus_tag="ACG8MG_000266"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFQ0374345.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(30982..32538)
                     /gene="rny"
                     /locus_tag="ACG8MG_000267"
     CDS             complement(30982..32538)
                     /gene="rny"
                     /locus_tag="ACG8MG_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFQ0374346.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(32949..33998)
                     /gene="recA"
                     /locus_tag="ACG8MG_000268"
     CDS             complement(32949..33998)
                     /gene="recA"
                     /locus_tag="ACG8MG_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFQ0374347.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(34107..35348)
                     /locus_tag="ACG8MG_000269"
     CDS             complement(34107..35348)
                     /locus_tag="ACG8MG_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFQ0374348.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(35588..36166)
                     /gene="pgsA"
                     /locus_tag="ACG8MG_000270"
     CDS             complement(35588..36166)
                     /gene="pgsA"
                     /locus_tag="ACG8MG_000270"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFQ0374349.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(36375..37298)
                     /locus_tag="ACG8MG_000271"
     CDS             complement(36375..37298)
                     /locus_tag="ACG8MG_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFQ0374350.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(38037..39323)
                     /locus_tag="ACG8MG_000272"
     CDS             complement(38037..39323)
                     /locus_tag="ACG8MG_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0374351.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(39316..40584)
                     /locus_tag="ACG8MG_000273"
     CDS             complement(39316..40584)
                     /locus_tag="ACG8MG_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0374352.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            40836..41297
                     /gene="mscL"
                     /locus_tag="ACG8MG_000274"
     CDS             40836..41297
                     /gene="mscL"
                     /locus_tag="ACG8MG_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFQ0374353.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(41356..41802)
                     /locus_tag="ACG8MG_000275"
     CDS             complement(41356..41802)
                     /locus_tag="ACG8MG_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0374354.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(41895..42140)
                     /locus_tag="ACG8MG_000276"
     CDS             complement(41895..42140)
                     /locus_tag="ACG8MG_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFQ0374355.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(42255..42833)
                     /locus_tag="ACG8MG_000277"
     CDS             complement(42255..42833)
                     /locus_tag="ACG8MG_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0374356.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(42910..43440)
                     /locus_tag="ACG8MG_000278"
     CDS             complement(42910..43440)
                     /locus_tag="ACG8MG_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFQ0374357.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(43444..44304)
                     /gene="rlmB"
                     /locus_tag="ACG8MG_000279"
     CDS             complement(43444..44304)
                     /gene="rlmB"
                     /locus_tag="ACG8MG_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFQ0374358.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(44297..44692)
                     /locus_tag="ACG8MG_000280"
     CDS             complement(44297..44692)
                     /locus_tag="ACG8MG_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFQ0374359.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(44689..46101)
                     /gene="cysS"
                     /locus_tag="ACG8MG_000281"
     CDS             complement(44689..46101)
                     /gene="cysS"
                     /locus_tag="ACG8MG_000281"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFQ0374360.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(46098..46634)
                     /gene="epsC"
                     /locus_tag="ACG8MG_000282"
     CDS             complement(46098..46634)
                     /gene="epsC"
                     /locus_tag="ACG8MG_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFQ0374361.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(46720..46959)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(46956..48416)
                     /gene="gltX"
                     /locus_tag="ACG8MG_000283"
     CDS             complement(46956..48416)
                     /gene="gltX"
                     /locus_tag="ACG8MG_000283"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFQ0374362.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(48586..49743)
                     /locus_tag="ACG8MG_000284"
     CDS             complement(48586..49743)
                     /locus_tag="ACG8MG_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFQ0374363.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(49777..51147)
                     /gene="radA"
                     /locus_tag="ACG8MG_000285"
     CDS             complement(49777..51147)
                     /gene="radA"
                     /locus_tag="ACG8MG_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFQ0374364.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(51210..51737)
                     /locus_tag="ACG8MG_000286"
     CDS             complement(51210..51737)
                     /locus_tag="ACG8MG_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFQ0374365.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            51967..52773
                     /gene="proB"
                     /locus_tag="ACG8MG_000287"
     CDS             51967..52773
                     /gene="proB"
                     /locus_tag="ACG8MG_000287"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFQ0374366.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            52770..54017
                     /locus_tag="ACG8MG_000288"
     CDS             52770..54017
                     /locus_tag="ACG8MG_000288"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFQ0374367.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            54093..54437
                     /locus_tag="ACG8MG_000289"
     CDS             54093..54437
                     /locus_tag="ACG8MG_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374368.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(54554..54880)
                     /locus_tag="ACG8MG_000290"
     CDS             complement(54554..54880)
                     /locus_tag="ACG8MG_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374369.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(55495..56697)
                     /locus_tag="ACG8MG_000291"
     CDS             complement(55495..56697)
                     /locus_tag="ACG8MG_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0374370.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            56814..57329
                     /locus_tag="ACG8MG_000292"
     CDS             56814..57329
                     /locus_tag="ACG8MG_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0374371.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(57528..57974)
                     /locus_tag="ACG8MG_000293"
     CDS             complement(57528..57974)
                     /locus_tag="ACG8MG_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0374372.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(58021..59499)
                     /gene="msr(C)"
                     /locus_tag="ACG8MG_000294"
     CDS             complement(58021..59499)
                     /gene="msr(C)"
                     /locus_tag="ACG8MG_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0374373.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(59853..60482)
                     /locus_tag="ACG8MG_000295"
     CDS             complement(59853..60482)
                     /locus_tag="ACG8MG_000295"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0374374.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(60466..60930)
                     /locus_tag="ACG8MG_000296"
     CDS             complement(60466..60930)
                     /locus_tag="ACG8MG_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0374375.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
BASE COUNT        17049 a        13239 c        10918 g        20080 t
ORIGIN      
        1 ctgttgttga ttcttctgtt gctgaagatt caggcgctgt tgattcttct gttgaagatt
       61 caggtgttgt tgattcttct gttgctgaag attgtgtagc tgatgatgat tcttcagtca
      121 ttgaagattg tgtagctgat gattcttctg ttgctgagct ttgtgcagta gatgaagctt
      181 gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc tttttcagct ttttcttgtg
      241 cagcttgttg acgagcttgt tctgctagac gttgttgttc acgaatacga gcttgctctg
      301 cttcagcttc tgctttttga cggttcaagt cagctttctt gtcttcagca gttgcttgtt
      361 cagcagctaa tgaagttttc agtacgttca aatcagcttg tttagataaa agatcgcctt
      421 tttgagattc caaagcagct tggttttcag ctaattcttt tagtttagca tcgttttcag
      481 cttttttgtc ttctacagct tttttatctt gtttttgttg ttccatcaag tcgttgtttg
      541 cttttaccat cgttgtcatt gcttgaacgc gtccaatagc atctgctaat gaatcagcat
      601 tcaatacagc atcgatgtag tttgagcttg tgttgcttac ttgtgcttcg cgtgcttgtt
      661 tttggattgt atcttcacgt ttttcaatac gttcttgtaa atctgcaatg tcttttacta
      721 attgtgcaga ttcttgacgc aatgtatctt gttttgctaa taggtcttgt gcttgtgtgt
      781 tgatttcaga aacttggctt tctaaggcat cgatttgtga ttgtgcatct gcttgttgat
      841 ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg agaatcaaaa tcgtccgccg
      901 cggcagcgat cggtgatgct acggcagtca aggtcattga acataccatt actgctgata
      961 ttaaactctt tttcactctt cattcctccg actggcttaa tttaataaat atctaaatat
     1021 atttaagtct ttcttaacga tataaaaatt gtaccatagc agtgtgtcaa cgatataata
     1081 gttatgttac aaaaatgttt caaaacaact tttaaaaatg acaaaactgt tcgtttcaga
     1141 aaatgatcat gaaaaagccg cgttaagtct agcatttctt aagataacaa cgattctttt
     1201 tcgctttcga cctaaaaata aaaaaagaaa ttattcatca ctgaataatt tcttaaaggg
     1261 aaaaatgaat aatacaaata taatcatgtt caacagcagt gtaggtccta aaaaccttgt
     1321 aataaaatac gtgttattta ctactgctaa tttaaaaatc aactggacca ccatcgtaaa
     1381 caattcataa cccgtgacaa agatgatcat actgaaaaat tcagtaaaga tgttgacatg
     1441 gataacgtct ttcattacat acatcaacca tacaattaaa gggagagcta ctgcatagat
     1501 accgataacg ccaatatagt aagcgtcata gatagccccg agaacaatcg tggtaatcag
     1561 taaataacgt ttttcaaacg ccatactgca acataataat gccaatatca gaaagtgggc
     1621 atttgacata taggtgcctt tcgaccactc ccctagcatt cttgttaaat gaccatctat
     1681 caacatcaaa aagaacaaaa cgattggcag atagtacttc attgtttctt tcttcaacat
     1741 ggactaatcc tccgctagcc gttggacaat cgtcacaact gacacgtcat acatttctgc
     1801 ataaggtttc acataaactt cccgatccag cccataacta tctggtttcg ttttaataac
     1861 cgtaccgatc ggcaagttag ctggagaatt ccctccaaga ccggatgtct ggacaacatc
     1921 cccttctttg atatccgtat caccagttaa ttgggtcacc actaaggcat ggagcttttc
     1981 atcatagttt ttaagcaaac caaacgcttc gccattagcc gaagataccc gtactggaaa
     2041 atgattggag ctttcattag aggatgacag taattcgatt ttagacgaag ccgtattgac
     2101 ctcgatcact cggccaatca atcctttttg cgacatgact gccatatttg cttcgatacc
     2161 gtctttagaa cccttgtcta cgatcagcat gtcctgccaa ctgtcaggcg aacgagtaat
     2221 gacatttgcc gttacttttt cgtaactagt cagcgtctga ttcaactcaa gttctttttt
     2281 caacgcttcg atttctctct cgtaattctt gttttgctgt gaaacgtcat catagctatc
     2341 gattttttct tttagccgct cattttcggc ataagttgaa aaaagattat ggacagaatc
     2401 tactccattt cgtacccacc gtactggggc agagattgtt ttatcaacca ttgcaacact
     2461 gtcattaacg atggattgga agaaattggg ctttccttca ttcgctcgct gcgcagcggt
     2521 cacacttagt atcgtcacca cgatgataac aataaccaag gtaataataa tatttttatt
     2581 aggattgaac ttcttcacaa agacacctcg aattaattag actcttgtgt tcaattttat
     2641 cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta agcttttttt tagcaaataa
     2701 tagattctgt catttttagc ctgaaagaca aattttttta tttgtttatg gatcaatctt
     2761 tgctttgaat aactgcaatc ataccatctg aaaaagaaaa ttctgcggta tctgaaagaa
     2821 attcgttact tgcataagat gtcggatatt ctacttcttt attttccaat acatacttgc
     2881 tgccttttaa tgtaagattc gagacgggag tcaagcagca ataagctaaa tacttcatat
     2941 ctggtatctg tcttactata tgctctccag gcaaataata agacaaataa ttttgacaat
     3001 ctctgataat aagctgatgg gcaaagggtt tgaagcgagg ttccatacct aaccagagat
     3061 tggctaaaag atgatccaat cttccgcccg tagctccaat caaagtgact tccgcttgcg
     3121 ggtaacgttc aaatgttttt aagataccta actgtgtatc tgtatcgtct ttttctgcag
     3181 gggaacgggt atactctttg gcttttgcaa atacttcctt ctgctcttcg ttcgacaatg
     3241 aatcaaaatc acccactgcc agatctactg ggtagcctgc ttgaagcagg tacagcgcac
     3301 cacggtctat cccgacatag gcatcgtatt tggacagcag agactgcggc cagttttgcg
     3361 gattacctcc agcagctaat aaaattttca tttagtctaa tgcctctttt aatcgttcga
     3421 tttgtccgac tggattttcg gctccataga tatatgatcc tgcaacgaag acatccgccc
     3481 ctgcatcttt acagattttt gctgtttctg gaacgattcc gccatctact tcgattgcat
     3541 aagtccaatt gttctctcta cgtagatcag cgagttccgt aattttttct actgtttctt
     3601 caataaagct ttgtccgccg aacccaggat tgacagtcat gactagtact tgatcagcca
     3661 aaggcaaaac atgtttgatc atggagactg gtgttcctgg attgataact actccagctt
     3721 taactcctaa gctttttgtc atttgaagtg cccgatgaat atgaggagtt gcttcgacgt
     3781 ggacagtaat gatatctgct ccagcttttg caaatgctgg aatgtagttt tctggatcaa
     3841 cgatcatcag atggacgtct aacggtaatt tagtaacagg acggattgcc tgaacaatat
     3901 ttggccctaa tgtgatattt gggacaaatc ggccatccat tacatctacg tggatgtaat
     3961 ctgcgcctcc tttttccact aactcaatgt ctctttgtaa attggcaaaa tctgcactca
     4021 aaatggatgg tgctatcttc attcttgttt cctcctattt tcgtttacct tctttttttg
     4081 catacattgg ttttcttttt tcgatttctt gtaagaattg caaataattg tcgtaacgtg
     4141 tctgtgcgat accacctgtt tctactctat gttttacttc gcagccaggt tctttagcat
     4201 gcatgcactc tctaaatttg caatggacag aagcttctac aaattcagga aattgtctcg
     4261 gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct aaatcctggt gtatctgcta
     4321 ctaacccgcc atagatagga atcagttcca catgacgtgt tgtatgccgt cctcttccaa
     4381 gtgcatcaga aatctctcct gttgctaaat tcagttccgg tgatatttga ttcagtagcg
     4441 tcgattttcc tgctccagac tgacccataa agaccgatag ccgttctggg aagtaacgga
     4501 tcagttctgt cgtatcaccc gtatgattgg gtacaatcac cggatagcct attttagtat
     4561 aggtttgctt gatctcttcg attttttctg ggtcgtctga caagtcggtc tttgtcaaaa
     4621 aaataatcgg ttcgatcgac tcgtattcca aggtaactag aaagcggtca agtagattat
     4681 aagaaaatgc aggctctacg atacttgtga caatgattcc aagatcaaca tttgtgactg
     4741 gtgggcgaac taattgattt tttctcggca agatttccag aagatagccg tctgtttgat
     4801 tttcactctc aaagatcacc tgatctccga caagcggcgt gattttccga ttccggaaat
     4861 ttcctcttcc tcttgtttga taggtctctc cgtttgcgta tacatagtaa aaaccgctga
     4921 ttgcttttct gatttgtcct ttgataagtg ccacctccgt ttttgtttat tttaccatac
     4981 gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa gtatatggct tgaaaattga
     5041 tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat caatgtcgca ttcccttctg
     5101 atatatttta ttcagcactt attttgaact ttctgaggtg ctagctgatg aacttgtgga
     5161 gctggaagat tcttcttttg aagagttgct tgtgctactg gagctacttg agctgctaga
     5221 actgtctgaa gagtcttttt cagaactaga agacgctgga tctggtcctt tagagtaaat
     5281 tacgttcaca accattcctt tggtaattgt cgttcctgcc cctggtactg tacgaatcac
     5341 tttatcttta tccaccgtgt ttgaatattc ggaagtctca tgcccgatat attctgcacc
     5401 gacactagct aaataacttt gtgcttcgct ttttgtatag ccggagatat cattcaattt
     5461 aggctctgtt ccttggctta cgacaaaatt gatgtaagtt tcttctgcta caacttcacc
     5521 cgaggctgga tcctgactga tgatcgaacc tgatggtaca gtgtcgctat agtcataagt
     5581 agcactaata ttattgatat tgaatccttg tgtaacaagc caattacgaa cagtttcgta
     5641 gttttctcct acatgactac tcaaagtcac tgtttctggt cctttgctga ctgtgaaagt
     5701 aatcgtgtct gttttaggat cgacttctgt atcttctgat ggagattgcg aaatgatcat
     5761 tccgctttct acctctgaac tgaattcctc ttctttttga atcaagtcag cagaaaaccc
     5821 taattctttc aaacttgctt cggcatcttc ataactttta ccactgtaat catctaattt
     5881 gatttttttc gtgccgctac taatatacag agtgacttcg cgtttttgct tgactgtcgt
     5941 tcccgctgcc gggtctgtct tcactacatt accttcttcg attttgtcat ctgcaatttc
     6001 ttctgttttt gttgctactt ctaatttcgc ttttgcgagt gtttctctag cagaagcttc
     6061 agaaagatca gaaacatcag gaacaacaac ttctcctcgc ccgccagaag caaagaatac
     6121 agctgttccg attcctaaaa cagcaagaat agctaatagg atcagccacc attttttctg
     6181 tcctttcttt ttaggatcag gcagaacttc ttcctcctct ggtaaggttt cgttttctgg
     6241 cttgtctttt ggcagaggca tagatttgaa tgaatcaggc attggtgcat cttccgtaag
     6301 gggtgtgata acttttgttt cattatccat cacatgaggc tgccattttt cttcgtttgc
     6361 acgagctggt gataatactg tagaaagatc atcagacatt tcctcggctg acttataacg
     6421 gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt tccagtgctt gtggcacatt
     6481 tggatcaagt gcttttatag atggaagatc atcttgaaaa tgttttaaag caattgttac
     6541 agcagattca ccatcaaacg gtacgctacc cgtcaacatc tcataaagga tgatccctaa
     6601 agcataaata tctgactgct tcgttgccat acttcctctg gcctgctcag gggacagata
     6661 atgaacagat cctagcatcg tattcgtctg cgtgatcgat gtttcagata aagcgatagc
     6721 aatcccgaaa tccgtgattt ttacaacacc ttcattatcg atcagtacgt tttggggttt
     6781 caaatctcgg tggatgattt gatggttatg agctaaagaa atagcagaaa ggatctgctg
     6841 cataatattt actgctgttt cataaggaac aggataatgt gtttggatat atcgtttcaa
     6901 gtccattcct ttgacgtatt ccatcaccag atactgcatg ttgtcttctt caccaacatc
     6961 gtaaacacta acaatgtttg gatgaacgag ttctgttgct gctaaagctt ctctctgaaa
     7021 tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt aagactttaa ctgctacatc
     7081 acgatccaag attaaatcgt gcgccaaaaa tacatttgcc ataccaccgc taccgatatt
     7141 tccagtgatt tgataacggc catttaattt tttgcccaac tcgatcatac actgtcctcc
     7201 ttatattcga taagaagaac ggtgatatta tccgctccgc ctgcttcatt ggcacgatca
     7261 atcagctcag ttactttatg agacaatggg ccttcttgat tgatgatcgt tcctatttct
     7321 tcttcagaca gcatgttcgt caagccatct gaacataaca gcagtcgatc ttttagttgc
     7381 caaagatgag tagaaacatc tacttcgact gttccaggca tgccgacaga acgtgttaaa
     7441 atatttttcc ttggatggtt cactgccatt tcttcgctga tttctcctga tttgactagt
     7501 tcattgacta aagaatgatc ctccgtcaat tgcatgattt tatcttcacg aatcaaataa
     7561 gctctgctat ccccaacatt ggcaatcgtg aaccagtcat ctgacagagc ggccgctacg
     7621 attgttgttc ccataccgtt atattccggc tgttcttgtc cacgctgata aatcagcata
     7681 ttttcttctt gaatcgattg aatgaaccat tgggcgattt tttcatcgtc aacagcttct
     7741 tgtttttccc aagcttcgcc tagattcgtt accgccatct gactggcaat gtctccggca
     7801 cgatgaccac ccatcccgtc tgctaataca gcaagcttga gacctgaacg gctggtaaat
     7861 acactggcat agtcttgatt tgtgttacgt cgtcttccta catctgattg atattcaatc
     7921 tgcatttttt cacctcatta ctttttgcgc aaacaacaaa tgaaaaatcc atctgtgtaa
     7981 aactgatgcg gatacaaagt caacattttc tcttcgattg atgattgtag cataggattg
     8041 gtcaaaacat caatcctctc aaaatcaggg tgtctttcca agaaagcaga aacaacgtct
     8101 tggttttctt cttttaagat cgtacaagtg ctataagtca gaataccgga agactttaaa
     8161 gttggtgcgc agctttctaa aatttccaat tggatttttg gtaaattcgc taattcatcc
     8221 ggctgttttt tataacgaat atctggtttt cgccgaagca gcccaagacc tgaacaaggc
     8281 gcgtctacta aaatccgatc aaactgctct gcttcgaact gtttactgac ttctctagca
     8341 tccatctttt gagcgtagac agcagaagag acacctagac gtgctgcatt atcttcaatg
     8401 agtttgactt tgtgttcgtg tacatctaaa gcgaccactt ctccaccgca tgacggatct
     8461 aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg cacaggcatc taagacatga
     8521 tgttcaggct cgatttgcat agacggcgct acaagcatcg aactttcatc ttggatcgtc
     8581 atgacgccat cgtggaacaa tgagctagaa gctaaatgcc cttttttcgc tactataccg
     8641 tatggactga cttggctttt ttcagctgtg atctcttctt cttgcaaaac agtgatggcc
     8701 tgatctctgg aaagtcggcg tgtatctact cgggcactag catggctagg ctgatatagc
     8761 gataaaccga gcttttcagt ttcttcgtat ccgatttgat ccaccagtcg tttcgtcatc
     8821 catagaggca ggctgatttt gacagaaagt ctttcgatag gatcagcaat gccttgcaga
     8881 tctggtgctc cttgacgctg ataattcctc aagacaccat ttacaaattt ccctgtccca
     8941 ggatttcctt ttacttttgc gatttcgacc gcctcgttta aaatagcatg tgcagggact
     9001 ttatctaaaa attctaattg gtatacagaa agataaagaa gcatcttgac ccagtcatcc
     9061 actttttttg ctttagcgat caatggtgca acgtaaaatt ccaatagtcg ctgatggcta
     9121 atcgtaccat agatcaattc tgtaaataaa cccacatctt tttgatttag ctcgctttgt
     9181 ttgatcatct cattcaacaa aagattcgag taggcgccac ccttgttcac tcgctctaat
     9241 gtcagtaatg ccatatatcg tactgatttt ttcatttttt tagggatttt tttcattatt
     9301 tcacctgctg accaatctga acttgctgac cgcttccgtt aaggaaatca tgtacggcct
     9361 gttttccttt accagctggc tgtaattcat tgatggctaa gacggtctgt ttaccgcaag
     9421 caatccatag attttttttg tctttccgga tgatcgtacc aggtgctttg tctgtagttt
     9481 ctgccaatac ttcaaccgat aataatttcc agcgtatgcc ctcataggtc gtgaatgcaa
     9541 ttggccaagg acgcattccg cgtacttggt tgtcaatttc ttctgcagtt ttatgccaat
     9601 cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt tgcttctgac tcgtcttgag
     9661 gtactggttt ttgtccatcc aaaatattgg gcaatgtttc tagcaatagc tttcttccta
     9721 aaaggctaag cttttcaaac atcgtcccta catcatcttg tttagtgatt ggtatgcttt
     9781 cttgcgcata gattccgcct gcatccattt ttttgatcat ttccatgatc gttacaccag
     9841 tttctttctc cccattcatg atggcataat gaacaggcgc acctccccga tatttaggaa
     9901 gtaaagaagc atggacattg attgccccta attttggtac ttgcaataac ttttcaggca
     9961 agaattggcc aaatgccgca gtgacgatca catcaggcac caattcaatg atttcttcca
    10021 tttctggcga accggagatt ttctctggct gcagcacacg gataccgtgc ttcagagccg
    10081 ctttttttac cggtgtcggc gtaattactc gtttccgccc aactggacgg tcgggctgtg
    10141 ttaccactgc ctggatctcg taacctgatt ctatcagccc ttcaagaatc ggcacagaaa
    10201 aagcaggtgt gcccataaat actagcttag tcatcaagat gttcctccat atattcttca
    10261 agttcttctt ccgggatctg ctgtatcatt ttctcaatga acaagatacc gtctaagtga
    10321 tcgatttcat gctggaaggc acgggctaaa tagccaaaag ccgtcacttc tatctcttct
    10381 ccgtctcgat cataataacg cacggtcact tcatctgctc gcttgaccgt tccatagaca
    10441 tgcggaatac tgagacaacc ttctacgtca aggctttcac cttttgcttc gatgatttct
    10501 gggttaatca gttcaaattt ctcgccttca tctacttcga tcacagcaat tctcttgttt
    10561 tgtcctactt gaggagcagc gatccctatt ccgtcattcg caatcattgt ctcatataaa
    10621 ttgtctaata aagcgattgt ctcgtccgtg attacatcga ttggctgagc agttcttttt
    10681 aatttatcat tagggtggat caatatagga tagcgcataa tttggccctt tcttaaataa
    10741 agtttaatgg ttcgttgtca attgccacaa aaagaccttg tcgctgttct ttctggcttc
    10801 cattcaaaat ttcatccaaa acatgaggaa gattcggttc atgtttatat ttgatgatta
    10861 gttgataata atatcggttt ttcactctca aaatcatgct gggcgttggc cctaatagca
    10921 agctttgagg gcttaacact tcttttagct gattggcaat ctgaaagatt ttctttgccg
    10981 ctacttgttc ttcaggatgg ctggcggtta ttttcacagt aaagtaataa ggaggatagc
    11041 ctccacgatg ccgtacatgc atttcctgct gataaaactg ctcataattt tgttttttag
    11101 ccaattctat ggcgtaatgt tgaggattga atgtttggat cacgacttcg cctgcttttt
    11161 ccgctcgccc tgctcgtcct gaaacttggg taagcaattg gaatgtatgc tcactagaac
    11221 gaaaatccgg taaattcagg gaggtatcag cattcaacac cccaactaat gtcacttctg
    11281 gaaagtccaa acctttagca atcatttgtg tccctaataa tatatctgct tcttttgcac
    11341 cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg agtggtatct acatccatac
    11401 gcaaaatcct agcttgcgga taaagttctc ggagttcctc ttctactttt tgagtacctg
    11461 ttccatagta gcggatttta ttgccgccac aattaggaca gcgattggga ataggttctt
    11521 catgtccgca ataatggcag cgcattgaac gtgtatccat atgcagtgta agagagatat
    11581 cgcaattagg acatggaaga acaaatccac agtccctaca catcacaaaa gacgaatatc
    11641 ctcgacgatt gagcagtagg acagtttgtt cttttttgtc taatcgattt tgtatttttt
    11701 cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg aaactcttca cgcatatcta
    11761 cgatctcaat agctggaagc tgtgcagctg ctttggctcg gtggtttaac tgcaataact
    11821 gataaacgcc tttttgagct cgtgctcttg attctaaaga gggcgtcgcg cttcctagaa
    11881 ctattgggca atgatgatat ttactgcgcc aaattgctag atctctcgca tgataacgcg
    11941 gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc gataatgatc agcccgatat
    12001 ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac tacttgcgct tcttcccgct
    12061 caatcttgcg ccattcatca tatttttcac cttgggaaag tccgctatgt aagaccgcaa
    12121 ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg cggagtcaat gcgatttcag
    12181 gcactaacat aatagccgtt ttattttctg acaaaacatc agcgattgcc tgaagatata
    12241 cttctgtttt cccgctgcca gtgattccct ccagcaaaaa gacctgatct ttttgctgct
    12301 gacctgctgt gatgatttgt tcgacagcat ttttttgttc tgcattcagt tccaatgccg
    12361 tcgtttggtc aaatactcgg tctttataag ggtcgcgata acgttctgat tctataaact
    12421 ccagccagcc cttttctttt ccttgattca agacagcagt actgattcct ttatttttga
    12481 aataagcaat tggttcttct tggctcagtt cagataaaca caaaagaagt tctttttgtt
    12541 tcttggcatt ttgccgtaat tttgcccatt cttcttcgat ttgcttttca gttaacaaag
    12601 aacggaccaa acgaatcgtt tttactttat ttcgtgtatg gacttcatat ttgatatcta
    12661 ctaattgctc ttggcgccag cgaatcaatt gagaaaggct gccttcttct tctgcttcct
    12721 tccaggaaat cacttctcgc tgatcaaaca atacctgaac ttctggtgtg tctgccaaag
    12781 gatagatcaa tttatcatat tctgctttca tcacactcgg aagcatcgtt tgtagacaag
    12841 tgattttgaa tgcgtaagta atttttttca tataatcagc taatgaaagt aattcttcat
    12901 tgaccaccgg agcaaggtct agcaaacgaa taactgcctt caaagatgga ttagctgatt
    12961 cttctgattt ttggatagcc ataacaaatc cttggacatg acgattgcca ttaccaaaag
    13021 ggacttctac tcgcatcccg acttgcaaag ctgtttccat ttcagaggga accaaataag
    13081 tgaatggctg atcggtctgc atagtaggta cgtccacaat gacttcagct gttaatgtca
    13141 tggttcgttt cctccttttt ttgtcttttc ttatcaagcg acaaaaagtt ccttgcctat
    13201 tttactagag atcgatggat ttgtcttatc ttctgccaaa tcctaacaaa aaattaacag
    13261 cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca cagccttttc taccagacaa
    13321 taagtggcga atgtttgaaa aggaaacagc tgggtttgct tttcttttta gatgttcgct
    13381 ctatttttct ttattacatt ttttgctctt cacggatacg tgattccaat tcgtgttgtt
    13441 cacgctcttt ttgtgcttga cgttcctctt cttccatctt caaacgttca cgttttaatt
    13501 caggatgtgg atcattgact acatttcctg catcgatttc ttctaaagct tgaccgacgc
    13561 tttttactga atcaaaagaa tctagagtag gctgtgcact tgcatccaat tcatgcgcgc
    13621 gtttacttgc taaaatcact agagaatatt ttgaatttac acgatctaat agtgagtcaa
    13681 ttgaaggttt taacatcata agactacatc tcctttaaca ttttaatata tttaccgatt
    13741 acgcgatcta ctcgaaaatg ttcactggca ataatgtttt tgattcgttc aactgctagc
    13801 ggcacttcat catttacaac agcatagtcg taaagagcca tcatttcgat ttcttctttt
    13861 gcaactttca ttcgtttttc gattacttca tctgcatctg ttcctcggcc tacgatccgt
    13921 gattttaatt ccgctaaatc tggcggagtc aaaaagatga atacaccatc gggcactttt
    13981 tctttgactt gttttgctcc ttggacttca atctccaaga aaacatcttt tccttcatcc
    14041 aatgtcttgt tgacatatga caaaggagtt ccataataat tcccgacata ttctgcgtat
    14101 tccagcatct ctccagcttc gatcattgat tcaaactctt ctttgcttcg gaagaaataa
    14161 tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag tcattgagat cgaatactga
    14221 aaatcattat cctcactctc aaaaattgct tttcgtactg tgccttttcc tacaccggat
    14281 ggaccagaca acacgattaa taatccacgc tctgacatga cgacgtcctt tcaactagtc
    14341 tttttgtact tatcgtttat tttgcacttt ttttctgcaa ctttcaaggt tatctttaat
    14401 tatagctaga aattggtaaa aaagctaccc tgtaccaaga ggtcgtaaaa aagctgtcct
    14461 gcatcaagta ataagaacag ccaaacataa acagcttctc atgtttttcg tttggttggg
    14521 gttattatcg cagatgcaag cttttgaacg ccgtgtataa gaggtcgtga agaagccgat
    14581 tagtttcgag tatgaagaaa aattttgtga aaatagtttc tcacattttt gcataaattt
    14641 ggcttaatac cgagaaaatg gtttttgaac atcacttatt caaaataaaa aaagattgtg
    14701 acacgaattt tgtcacaacc tttaaatgta ttcctagagc aaagaagcta agcgaagaaa
    14761 tccaattctg tttatttatt gtatttcttt gttcagcatc cttgctaaaa gcccatattg
    14821 gacaaagtat tcactacacc catgatctca tcttttcgag aacgtgcttt ttctgtcgga
    14881 tcttctacct cttctgtgtc aattcgaaca ataaatttcc gattcatcgt cacgtaagca
    14941 ccgtcgttga tcaaaataaa cagttcgttc tcattatagt ctgcacaggc atctttccat
    15001 acgtcaaagc cgtttttttc agaagggata tcccgtttaa ccacagtttt tccacttaat
    15061 tcgtggaacg ttacgtcata atatttcatt atctctacct ctttcgtcat tcaatatttt
    15121 gtacttgttc tctgattttt tctaagatcg tcttagactg gacgacttga tttttaattt
    15181 cgattgcatt tgatttggaa ccgatcgtgt tgatttcccg attcatttct tggatcagga
    15241 aatctaattc ccgacctaca ggacattgtg cttcgataag ttcatctagt ttttgtacat
    15301 ggatcgccag acgatccagt tcctcgtgaa tatctccgcg ttccaacaag atggctagct
    15361 ccgtcaacaa ccgttgctca tcgactttcg ctccaagaaa ttcctccagc tttttttgat
    15421 accttatttg aaattccttt tcatagattt ctacaaaact atcgagtttt tttagtactt
    15481 ctttgaattc ttcagtattc tttttcaaca ctaaagctaa agcttgacct tctttttctc
    15541 gactcgcctc aatagaatca agtgcgcttt tcaatgtatc tagagctaac tgtgccaatt
    15601 cttccatgct ctcttccggt ttttcttcta ccgttacgaa gttttcgtcc agcacgaagc
    15661 gttccgccat ccgactaatt gacacttgat tttgactacc gtatcgttgt tgaattccgt
    15721 ctgtcaattg agtgataagt gaatcaatca attcccagtt gatgacgatc tgtttttcgt
    15781 ttttgccaat ataagctaga ttcacaaata cttcgattcg accgcggctt aactgctgtt
    15841 taataagctg acgaaaatcg ttttcaacaa agttcaggat tttaggactt cgtatttgaa
    15901 catctagaaa acgctggttg acacttttta tttcaatatc taactgatac gcttttgttt
    15961 cgcggatggc tttgccaaaa ccagtcatac ttttcattcg acagggtcct tcctcattac
    16021 atattttcta ttgctttttg cagttgttca aactcttcgt tcgtcaatgt caggcctttc
    16081 cccatttttt catggtctgg cgcccaatct cgaagatcga attttggcgg gcgaccattc
    16141 cagctgacta ggttcagttc tttacgccac cccttgttgt tttctgacaa tatagcgatt
    16201 tcttctacga tttcatatga aaattcttgt gccatggttg ttcctccttg agataatata
    16261 aatataggat cagtaatgtg tcaactggtt gcttaagcaa ttcatgctgc aaacgatcct
    16321 tgcgttttcc ttgtagcaga taatcaaaat aaacagtttt cgtgcgctca ttttgtattt
    16381 tttgctggta ttttcgatac cttcgccatt cttcagtact taattgacca atttgtgcca
    16441 ataaatcatc cggcatactt tttatatctt gttggcagat catttgaatc atcatgtcta
    16501 cttgatagat ttcattgggc agtaaaaaat gtttttttgc taaaagttct agcatcgcca
    16561 atccgcatgt ctctggtacg accctgttct tatataaatt acgcagtttt tgccaaaatt
    16621 ttatgctttt gaaaaaagat aattctaact gaatttcttt tgttaagtaa gacaaaaatc
    16681 ctcgccagta tgttcttgca ggtttaacag aatgaaatgt ataccagttc ccatagtaat
    16741 agcctaatcg ttttttcatt tccagatagc ctaattgtaa atttaattgg ttcctttggc
    16801 tatcaaaaag cagaaagctg cctagtgtcc acaatgtttg acatttccaa tggataaaaa
    16861 tgtctggcat acgaattttt ttcgctgggc ctggcccttg cacatctacg acaaatgcct
    16921 ctgttgctcc tttgttaatc gcaatatcaa taggtatttt gttgcgatac ccaccgtctg
    16981 catatttatt ttttccgatc tttcgataag ccatagcggg ataaaatgaa gcagaggcga
    17041 gtatccaatc tggtatttct tctgtttttg tctgttggat atcagtcact acttccgcta
    17101 gttctggcag tttagtagac accgtatata aacgtatttt gggattatgt ttgagtaagg
    17161 acaaatccaa attgtctttg accatctttt ccaatggagc agaagaaacc ccgccttcaa
    17221 ttagtgctgt tttcgtcatt tggcgtcttt ctcgatagaa gcgttcacga aggtcttcaa
    17281 ctgcagccat ttctggtaac gcaagcactt gtcttgtcga cagctttttc cataaattca
    17341 cagctttttt catatctcct tgtaaaatca atgcgccgtt caacgcacct accgaggtac
    17401 ctgtgataat ctcaaagttg atatcatgtt ccttcagcgc ctgccacaca ccaatctgat
    17461 aagcaccgtg cgctcctcct ccacctaaaa caagtgctgt atgatagacc agctttttat
    17521 ttaattgtgt ggttccttga tagccgtttt tatgaaaaat acgcaaccat tcttctgata
    17581 gaggagattg aaataaaaaa caacagtttt ctttaataaa aaaacgagcg caagcttcca
    17641 tataccgaaa aaattttttc cattcaaaag tttcaccttc aatcaacaga ttattaaaaa
    17701 taacatctcc tctactttta gcaatagtaa aatagacggt atattttttc gatactgctt
    17761 taaaagcaca ttgctttttt gcagaacgcc aaaaagcgtc tctactttct tgatgacttt
    17821 gaaaaaatgg aaggggttgt tttgcttgct ggataggcga atacagcgac attttctgtg
    17881 aaaagtaaac tttttttatc tccatcgcgc tctccttttt cttcaagtat accagtacgt
    17941 cataaaaagg tcatgagtct cgtattcgac tttgtagatt ccataagaaa acaattcata
    18001 aaacctcctt taaataaaaa aatacaaaaa tcacaaaaat taattgaaaa tgagagtgtt
    18061 ttcaatgaaa acgtgatttt gtaatggaat tttaaaataa aagaaacaag cttttttaat
    18121 gcaaatgttg taaaataaaa gagattaaga ggaaataaga ggaggaacat gatgactgag
    18181 tcagtttatg tacatattga taccacaagt aatgctgttt taacaaaagg gctgactgct
    18241 actgactttg caaacagtat cgtgcatttt cctcaaaact tgctcttgct tgatccatct
    18301 gcttctgctg gagaatatga atcccataca gggctgaaag taatccgtgg tacagaaaat
    18361 attcagcgtt tcttttcctc ttctcgtaac aggcgtggat tcgatgaatt gaagtggatc
    18421 gactttactg atttaacgat gctgaaagaa ttgacacctt tagaaatttc agaattatta
    18481 tattttggtc atatgaaaac acatcttcat tcgccgtttt tttataaact acagaataat
    18541 tttgtatact tcgatcttaa tgatcagctg aatcggattt attatcgcta tttagatgaa
    18601 ttttatagga tttttgctag taaattaagt tcgatcttgt cagataagat caatgtaaaa
    18661 aaatcttttt tcaaaaaaga aacacctgtt gaaaaaatca gtgtggaatt attgaaagaa
    18721 atgcgtaatt tgatgcagga agggatcatt ttttcttttg atcaagttgg tttgatcgat
    18781 ggtaaatatc gtatcccaat ctatgtgatt gaagattata tttggaaatt aaaaggcacc
    18841 cggtatcgag aagaggaaat catcgctact ttaatctact ctgtcaccga tcgacactgg
    18901 caattggtag aagatgccga tctttcttat ctgccattga agtagatcta tttcttatca
    18961 aaaaataaaa aggaaacaag aaactaggac atgattctgt ctcaagtttc ttgcttcttt
    19021 tttatttaca gctcctgtcg tttatctggc acaaaaatcg aaggagtcgt ttgttggatc
    19081 cctaataaaa cgatgatcgt tacaatagct gtactcaacc cgctggcacc attcattacg
    19141 aaagaaaaga gccaaggatt catcccccat agtgcatacg ctccccagaa aaccactcct
    19201 gcaataaaat gccaaaaata tctggaaagt gcgccaatca atgcaccaaa cacgatgctt
    19261 tttctaattt gtttcgtatt atcgtctaca tagccttttt ggacatttga agccatgagc
    19321 cctgctgcgc ctgcaaaagt ataggcaatc gggtactcaa tcaacacttg aacgacactt
    19381 aaataataaa cttgtcccaa cggaaaatgt aggatgcccc aaatgaaacc tgccagcatc
    19441 cctgcctttg tccctcttct taacgcaaaa agagtcaatg gaatctgccc taatgaaata
    19501 gaaaaactgc tgccgatctg gatcggaatc aacgacaaca ccatcgctaa cgctgcaaca
    19561 atcgttcctt ctacccaaac tctatttttt cccataaaaa aacttcctcc tttgaataaa
    19621 aaagtcacaa aggaggaagc agctttcgaa aatagtcaaa cagctgttta ttcacaattc
    19681 ctacgctcgc ataaccgata caggtacgaa gggtttagaa atagattcaa tctcagccca
    19741 attggactcc cctttgtgag tttatttatt atttattttt tgtttcaaac aatggactaa
    19801 ctggtttgtt ttcatggata cgtttgattg catctcccat tagtccgcca acacttactt
    19861 catcgatttt gtcgatttta cgatcttctg gaagatagat cgagtctgta acaactagac
    19921 gctcgatagc tgaatcttct attctttgta aagcagggcc ggataatact ggatgtgtac
    19981 atgaagcata cacactagtt gcaccagctt ctttcaaagc attggcagcc aatgtgatcg
    20041 tacctgctgt atcgatcata tcatcgatca gtacacaagt tttgcctttg acttgtccaa
    20101 tgatattcat tacttctgca acatttgctt ttggacggcg tttgtctatg atcgcaatcg
    20161 gagatttcaa aaattccgct aatttacgtg cccgtgtgac accaccatgg tctggtgaaa
    20221 caacgacaac atcatcgcct ttgattcctt tttctaagaa ataattggca ataagtggcg
    20281 cacccatcaa atgatcgact ggaatatcaa agaaaccttg gatttggact gcatgaaggt
    20341 ccaaagtcag cattcttgtt gcaccagctt tttgaatcat gtttgctact aatttcgctg
    20401 tgattggttc ccgtgcacgt gctttacggt cttgacgcgc atagccataa taaggcatga
    20461 ctacgttgat tgtttttgca cttgctcgtt tcagtgcgtc aatcatgatc agcaactcca
    20521 tcaagttatc attgactggg ctgctcgttg attgaataac atagacatgc gatccacgaa
    20581 tactttcttc aatgtttact tgaatttctc catcactaaa ttgagtaaca gatgattttc
    20641 ctaactcgac gccgactgct gctgcaattt tctctgctaa tgggcgattg gaatttaacg
    20701 caaaaatttt taatcttggg tcaaagtaat gttttgacat ggggacctcc actttctttt
    20761 tcacaactct agaattctac ctaataaata gtagtcatat tttttaaata aatcaagtga
    20821 ttttgcaaaa gtcttagttt agataaggga gtttttttgc atatccttct ttattgactt
    20881 gtcttgctct cgcaattgcc agcgcatatt ctggaatatt gtctgtaatt gttgacccgg
    20941 cagcaatcga actgttttta gctacctcga caggtccgat gatattcgtg ctagaaccaa
    21001 taaagctgtg gtcgccgaca gtcgtatgat gtttgttctt tccatcatag ttgacgaaga
    21061 caacaccaca tccgacattg atttcttctc ccagcgtagc atcaccaaca taagtcaagt
    21121 gaccgacttt agttcctttg ccgatttgag catttttcac ttccacaaag tttccgatat
    21181 ggacgttttc gccaatttca gcttttggtc gtaaatgtgc ataaggtccc acatctgcat
    21241 gtttcttgac atggctgctt tcaatgactg agttttcaat gacgacgtga tcctcaatcc
    21301 tactgtctac gatttttgaa tgtgacccga tgacacaatc agaaccaatg actgtatttc
    21361 cttggatttg tacacctgct tcgatcaatg tgtctggacc gatttctacg cctgcatcga
    21421 tgtatgtcgt tgctgaatcg acaaagctta cgccattgac catatgcatc tggttaattc
    21481 gctttctcat gatctcattt gctttagcta acgcgatccg atcattgact cccattgatt
    21541 cttcaaaatc atccgtttga taagcagcaa ctgtatggcc ttcttctttt aagatttcga
    21601 tgatatctgt tagatagtat tcaccttgag cattgtttgt tccaactttg cttaacgcat
    21661 cgaacaaagc ttcgttgtca aaacagtaag ttcctgtatt gatttcttgt acaagagctt
    21721 cttctggtgt tgcatctttt tgttccacga ttttttctac gatcccaata tggtcacgga
    21781 tgattcgtcc atatcctgtt gggttttctg cttgtgccgt caagatcgtt gcacttgcat
    21841 tttttccttg gtgatattca aaaagattgt tcagcgtttc agtagtcaat aatggtgtat
    21901 cgccactgat aactaacgtt gttccttttt ttccttccaa gaaagaagca gcttgcaaaa
    21961 cagcatgtcc tgttcccaat tgttctgctt gtaacgcata tttactgcgt tctcctagtt
    22021 gtgctttaac ttgttctgct ccgtgtccaa cgatcgtaat cacttcatct ggtttcgttt
    22081 cactgacccg attaatgata tgttctacca taggtttacc agaaacggga tgtaaaactt
    22141 tatataattt tgacttcatg cgggtacctt tccctgccgc caaaataatt gcataacgtg
    22201 cgtccaactg cgtcactcct aaatatagtt tgctgatttc agtttatccc agtataccca
    22261 ttttttcaac aaaacaagtg attttctttt atcccagcgt tttctggata aaaaaataga
    22321 tagaccataa cgggcctatc tatttggtaa ccggtgctct cctgccaact cctcggtcac
    22381 aagtcacact actcataaaa catgaaaagc tgttttttga aatcgtttct tgtgacttag
    22441 agttaaacgg taggcttaca acctcttcat taaacgtttt tcaatcagct gaccttcggt
    22501 attagctcaa taaacggcaa aatatgagga gcaatttttc cgtttattga gctaatactc
    22561 aggacaggac gctgatttca caacctctag ataaacggtg ttcaaaagcc agtttctcgg
    22621 tattaagcca aatttatgaa aaaatgtgga aagccatttt cacaaaattt ttcttactac
    22681 tcgaaactaa gcggcttttt cacaacctct tattctgaaa aataatttcc tggcacaacc
    22741 gtaatatttt tcgtttgagt atctacatct ttgacataaa gcagtgacgt ataatcatcg
    22801 atggcgcgat gaccgttgaa ttttgattcg gcaaatacag tgatgccaac aagttctgat
    22861 tcgaattctt caatcaagct cttcattccg ttgactgttc cgccgccttt catgaaatca
    22921 tccactacaa ggacacgtga accacgtttc aaacttctct tagacagttc catcttttca
    22981 attctttcag aagatccaga cacatagttg acacttactg ttgatccttc tgttattttt
    23041 gagtcccggc gtacaataac gaatggcgca ttcagataat aggacacagc ttgggcaata
    23101 ggtacgcctt ttgtggctac agtcattaca gcatcgattt tttcaccaag gtatttagaa
    23161 gcaatgattc ttccaacttg acgcagcagt tcaggttctc ctaacaaatc agagagataa
    23221 acatatcctc ctggaagcag acggtcttgt tctgatagac gttctgctag agattcaata
    23281 tattttttcg cctcttcata tgaaatttca gggatgaaca atacaccacc tgcagcaccc
    23341 gggattgttt ccaaggttcc ataccctcgt tctttaaatg ttttcttgat gattcccaaa
    23401 tcttcactga tagaagattt agcagattca taacgttcgg caaagcttgt taaggaaatc
    23461 aattcatgcg ggtgattcaa taaatactgt gtcatgtcaa ctagacgttc actgcgacgt
    23521 actttcacct attccaactc cttttaagaa attttatcgt tttacattat ataagttttt
    23581 cttcaaaaat tcgagcattt tatgaaaaaa taagaaaata gttcggtatt tattaaaata
    23641 aaaagaagcc tataaaaaat ttttcgtttt tataaggctt cttttcttta taatttagtt
    23701 attttttctt ctgaaggcaa ccaatattcg tttgatgata tttacaatca ggaacaaagc
    23761 aataaagact aatgtgattg ttgctcctgg aggtgtatct agataaaacg agctagttag
    23821 tcctgacagc atccctagaa atcctacaca aacactgata gcaatcacga cattgaagct
    23881 tttgcctaat cgcataccga ttgctgcggg aagaaccatg attgcggata tcaaaagtgc
    23941 accggcaatc gggatcatca cagcaatagc cacaccagta atcacattga acatcatcga
    24001 catcaaatga acgggtagcc catccacgtg ggcggtatct tcatcaaaag tcagaacata
    24061 catcggccgt ttgaataaaa agaacaatat accgatggca acgaataaga ttcctaaaaa
    24121 gatgacttgc tcccatgtaa tggtcacgat cgaaccaaac agataggatt ggatacttgt
    24181 tgctgaattc cctccgctca aattcatcaa tactaacgct agcgccaaac caccagccat
    24241 taaaatggca atggagattt ctgaataggt tcggtagatc gttcgcagat actccaatat
    24301 cacagctgct aatacaacga ctagcatggt cgtaagtgta ggattaaggt tcaaaaagaa
    24361 tcctaacgct acccctgcta aagagacatg agaaagcgta tcagccatca gcgattgtct
    24421 tctgatcact aaaaacacac ctagcatcgg ggcgattccg gcaatgaaga tagcggcaag
    24481 aaacgctctt ctcatgaatt catatgaaag caacgccatt gagaatcctc ctttctcacc
    24541 agtcggatct gtctgtccgc atactgtttg atatcttcat gatcatgggt gatcattaga
    24601 actgatttcc catggacatg cgcactatgt cggagaagac gataaaattc atttctggat
    24661 tcttcatcca tacctgttgt tggttcatca agaacaaata agtcagggtc agtagcaaaa
    24721 atacgcgcta aactgattcg ctgcttttgt ccgccagaca attcacctat tcgccgattt
    24781 cgcatatccc acatccctac agcttctaaa gcacgctgta catgttgatg atctttttca
    24841 gagagacgct tgaaccagcg gcctcttgga tatcttcctg aacggactaa ttcgatcacc
    24901 gtactaggaa aacctgcatt gaaagatgca acttgttgag gaatataacc gatacttaat
    24961 ttttttcctt cttgattttt ctcagcaatc tttatcattc cttttgaagg tttcagtaat
    25021 cctaacgatg ctttcaccaa ggttgacttg gctgctccat tttctccagt taaaatgaca
    25081 aattcacccg catctacatg ataagagaca tcttctagta caggttcatc atcatagtaa
    25141 aaggtcaaat cagatatttc gatataacgc accttacaac ctccattcta aaacttaatt
    25201 gatactttct tttaaagctt tcagattttt ttccatgaca gaaatataat tttcgcctgc
    25261 tttgatttgt tcattagtca agctttctaa aggattcaac acttctagct tcactcctgt
    25321 ttcattcgac aatgtctcag ctaccttcga agaggcattt tcttcaaaat aaatcacttt
    25381 gatgttgttt ttcttcacaa attcttttag ctcagctaag cgacttggtg tgggttcctg
    25441 atctggtgaa acgcccgaaa tcgctacttg ttttaagtcg tattgattcg ctaaataagc
    25501 aaaagcagca tgctgagtga caaatgtgcg gtttgttgca tcttttagct catcggagta
    25561 tttttgatcc agtgcttcaa gctttttgat ataagcatcg gcattcttcg taaaagtttc
    25621 ctttttatct ggatattttt cgcctaattc ttcagcaatc gttctgactt cttttatagc
    25681 taataccgga tcgagccaaa catgtggatc gtattcatgt gaatgaccat gcccttcttc
    25741 tgattcttca tgttcgtctg cttcttgcga ttctaaacgg tcgattcctt tagctgcttc
    25801 gatcatcaag gtctgtttgc tatccactga atcctttaac gaatccacaa aaaattccat
    25861 gtctgaacta ttatacacga aaacatctgc atcggaaatt tctgctcttt cttttgcact
    25921 tggttcgaaa tcatgcggct ccgttccagc agggatcaaa agtttcacgt ttccttcatc
    25981 ccctacgatc tcttttgtaa aatcatacat cggataaaaa gtcgtaacga tcgtcaaatc
    26041 ctcttttttg tctgcttcct tgttcgtatt tccacatgct gcaaacagta aagcacctat
    26101 cattaccgta gctgtcaaaa gatatttttt catttatatg ctcctttcaa tgaacaaatc
    26161 ggaattttac gatttattaa cgtgaactaa tgtatcaaaa taaaaaaaca cagtcaatag
    26221 aaatccatca atttctgatt taagcatgta tattcttgat taaatcgtaa aatggatgag
    26281 ttgctgctca tatctagcca tttcactaca ataaaaaact gaagaatacc attttaggat
    26341 tcctcagttt ttcatcacct attttacttc ttatatttta ttgcagggtt cgtactaaat
    26401 aaacttcctc acaaaaacct tttaacccat tatatactct ctgcgctctt gaataatgtt
    26461 gacacaaggc aaaaacggtt ggtccgcttc cactcatcaa tgcagcatct gctccgtact
    26521 tgatcatacg ctctttgatt tgatggatga ctggatgccg ctttcccgtt atatcttcta
    26581 aactgtttcc catttttttg atcatctgct gataatcttg ctctcggatg gcttcagcaa
    26641 gtccgtggat gtcttgatgt tggatggctt gcgtatctac ttctctaaac actgtacgtg
    26701 tagagacact cattcttggt ttgaccaaca caacccagca ctgtggcata gaaggtaata
    26761 cttggatttc ttcccctttg ccaccgacaa atgccgtact tccgtagaca cagtaaggaa
    26821 catctgtacc gacttccaaa ccgatttctg ccagttcttc taacgaacag cccagattcc
    26881 ataattggtt gatccctctt agagcagctg cacagtctgt acttcctccg cctaatccag
    26941 ctgctactgg aatattcttc ttaacaaaga tcttgacacc ggttttgata ttgtatttat
    27001 tttttagcaa taaagcagct tgataaacat tgttccgttc atctaccggt aaaaatgctt
    27061 tatttgtttc tacaatgatt ttgtcttctg ttatttcttc aaaaaataga tgatctgcca
    27121 agtctacgct cgacatcacc atttccaact catgataccc atcttgccgt ctatataata
    27181 catccagtcc taaatttatc ttggctggcg ctttttcaat caattccatc ctctcaccca
    27241 ttcgaaaagt ctaagtcgca agtattctat catagggaaa aataaaagta tagcttatgt
    27301 ctgcctagtg gttgattttg tcatcgtttc aacagctttc acagaatttt ctcttcataa
    27361 tccaaacaag ttctagtgat tcttataatt ctgtgagcac ttcttctgcc aaactcggaa
    27421 catttccttt ttcttttcct atcagactga gtagctgcat ggtagcaaat ccgaaattac
    27481 ttcctttcct ataagcaaaa taacgtcctt cccaatgatc gacatatttt aatttctttt
    27541 cataagcaga ctggaaggaa tagaaaaaat taccataatt atatacgata tgcatgactt
    27601 tttcttgaaa gaaagaaaaa tctgtttctc caacattagc taatggagct aagcctagat
    27661 tggcttccga gtagcccatt ttctcgtata caataaacag attggctaac agatagtcag
    27721 ctaattcctc agattcttta ggtaagaccc gcaacaaatc atatcccgcc tgcttcttcg
    27781 tcatcggctg agcggtgatg aagccaacga ctttttgatc tttacggaca ataccgacac
    27841 cgctagataa tacatatgat ttatcaaatc gtcccacaga gaagtatttt tctcgtgcac
    27901 cttgaagcca tttttgtgaa acttcattta cagcttgatg taattcttcc ggcagttgat
    27961 cgtaccaagt aaaatggtat ccttctttca tcaaacgttg atattcaagt tgctgactca
    28021 gccgagacaa gtcttctgtt gctagatcaa caatccctgc ttcacctact ttcgtgaagt
    28081 gaaaacccaa ttcatgaagc atcactacgt agctttctga tatcttataa aacgcaagct
    28141 gatagcctaa acaatctgct tgtttcataa actgattcgt tgctgcccgc atcattttag
    28201 gattgccaac tggatctcct aaaacaaagc atttgtttcc tttgatctga taaccaaaaa
    28261 cgacttgatc ttggtcgtct tcttggtaat aaaaataact gtaacctgac aagcgaagct
    28321 gatggcttgt agctgtgcct ccgtatgttt ccaataaacg gtcaaaacgg tctttttcgt
    28381 actcactgcc aagcttcgta gtatcttcag ctaaatattg ataaagcgcc actaaagcta
    28441 gcaatgagat gcccaaacca ataagccctg agaaccacac atcatctgaa ggaaacaaaa
    28501 taaaatgatt tcctgttatt cgaccgacta aaaagcgtcc gctgtgatag cctgcaattg
    28561 cgtaaacgat aaacaaaaag ccgaacaagc tagcatcaaa gattatagct ccccaagaat
    28621 aaacgaattt tgttcgataa aattcttttc tggataacca cacagctaac agaatcaaga
    28681 catataccaa tatcaactgc caagataacg tacgtgtgat tgtatttaaa ataccaaaag
    28741 atagtaaagc aatcgtaggc agaaatgcct ttttgacttt tactgaaagt gctctagcaa
    28801 ggccgagtaa taaaaagcca accaatagat tcactgtctg atctaaaaaa ttgaatgaga
    28861 acggcaataa tacttgaaat agccgactga ttgcagacag attagtgatt gttgatagaa
    28921 gtaccatcat gatccccgca aaatagacag cacttacgag cacgatatgc gcaacctttt
    28981 ggaaaaacag tcgcggaatg ttatctaaaa atcgattgat tttgactcct gtatgtgtga
    29041 tgaacaatag aagtcccgtc ataaaaggta gaacataata aaacaaccga taatataata
    29101 gccaaactac tgctacatct tgccgaacag acaattgact cattcctaag atcatcaaaa
    29161 cgtcaaaagt ccccattcca cctgggacca tcgtcagcat tcctatcaat gtagcaatca
    29221 tgaacatagg atagatcgaa agcatcgaaa tatctattct catcaacttg ccgatgatca
    29281 aaaataccgc aagggctccc gtccattgtc caaaggatgc cagtagcaga ccaagcaagc
    29341 cttttggata aaactcgaag aacaatgtct ttctctttag ataagcaaaa aagaaaatga
    29401 ctggtgctaa aaaactaccg ccgaaaagcc agatccaata gcttcgaaaa atactttctg
    29461 actgaataaa aaagacatca ataaatgtaa gaaacgacca tacagacaaa ccagtcagca
    29521 taaatagtgc gacttttgat acagtggcta aaaccatttt tctatttgtc tgctttccat
    29581 aaaaactagc ccgcagactt gctcctacca caccaccaaa accggcgaga ttgttgatcg
    29641 tgtttgttac ccacgcagca agaagaaact cttttctttc catttttggt tttccttgtt
    29701 tttctaagac tctgatcgtc acccaatcat acaacaacat cggagtgaca cccagcagtc
    29761 cagccagaat catgagcagg atcgtcatgc ggctttgttg tcccatcctt tgccaaatat
    29821 cttgccaact catgccatta gcaatatttg cgacttgatt tgccacaaag actaacacgg
    29881 agccgaaaaa aatcagtttt aaaatcagac tatgttcctt tatccattgc agcatcaagt
    29941 atcgtttatt tttcacattt tcccattcct aacttgtata cttttgtgtc ataccccaat
    30001 gttatcttat ttattttaat ttttccactt tagacctgtt ctttgcttgc gatgatagaa
    30061 aaagaaagat atcctaatgc taaaggagag gataacatga aagtaggatt tatcggcaca
    30121 gggaatatgg cacaagctat tattgatggt ttattaaaaa aaggaacagt ctcgaaagaa
    30181 gaggtgttcg tttccagtgg acattttgaa aatgcacaaa aatttgctgc tgaaactggc
    30241 gtccatgcat gcaaaagtaa tcaggaagtt gcatcacaat cagatatcgt tcttttagcc
    30301 gtgaaaccaa aaatcattga aaaagtattg gaagaactaa gagaatatag ccagaaagtc
    30361 ttattcattt ctattgcttc aggaaaatca ttaatagatt tagaaacagc gcttgaagat
    30421 cctcaggcct ccatcgtacg tgtgatgccg aatgtcaatg tcagcgtagg agcaggcgtt
    30481 tctgcgattt gcaaaaatga agctgccacg gaggaacagc ttcagcaagc tacgatgatt
    30541 tttgaagcaa tcggcagcgt ctatccttta gcagaatcag atttcagtac attcattggc
    30601 cttgctggca gctcacccgc attcgtttac atgctgatcg atgcgatggg acggactggt
    30661 gtactgaatg gtttgtccaa acagacagcc actgagattg ctgcaaaagc aattatgggt
    30721 tcttgtcaaa aattcctttc aagtgataaa aatccatggg aacttgtaga ccaagtttct
    30781 tctcctggcg gtacaactgt tgcaggtgta gttgccttag aagaagcagg attcatccca
    30841 gcaatcatca aaggaatcaa tacgacgatc gagaaagatc ttgagatgtt gaataaaaaa
    30901 taatgatagc aatgtttttt cgtgccttac aaaaaaagaa gagctgtggc ttttgccgcg
    30961 gctcttcttt tgtatttttt attatttagc ataatcagtt gcacgagttt cccgaataac
    31021 gattactttg atgtgtccag gataatccag ttcttcttcg attcgtttac gaatatcacg
    31081 tactaaacga acagcgtcta aatccgagat ttcttctggc ttgaccatta cccgaacttc
    31141 gcgtccagct tgaactgcgt aacttgattc tacaccggcg aagctgttcg aaatattttc
    31201 cagacttctt aaacgacgga tgtagttttc taatgattca cttcgcgcac caggtcttgc
    31261 tgctgagagc gcatctgctg cagctacaag gacagagata acagaagtag cttccacatc
    31321 tccatgatga gaagcgatcg cgttgattac tactgggttt tctttgtact tggcagctaa
    31381 ttccgcaccg atctcaacgt gagatccttc aatctcatgg tcaagtgctt tcccaatatc
    31441 atggagcaac ccggcacgtt ttgctaattg gatatcttcg ccaagctctg ctgctaagat
    31501 accagctaat tttgcaacct cgaccgaatg cttcaagacg ttttgtccat aactagttct
    31561 gaatcgcaga cgtccgatga tcttgattag atctggatga agtgtatgag caccgacttc
    31621 aaacgctgcc tcttccccgt attcgcgaat acgttcatcc atttctttac gagatttctc
    31681 gaccatttct tcgatacgcg ctggatggat tcgtccatct tgtatcagtt tttccaaggt
    31741 catccgtgcg atttctcttc ggattggatc gaatccggaa agaacaactg cttccggtgt
    31801 atcatcgatg attaaatcga taccagtcag cgtctcaagc gttcgaatat tacgaccttc
    31861 tcgtccaatg atacgacctt tcatttcatc attcggaagg gtaacgactg aaacagtcgt
    31921 ttctgaaact tgatctgctg cgcagcgttg aatcgctaat gataataagt ttttcgcttt
    31981 gcgatctgct tcttctttgg cacgctgttc agattctttt accattagag ttaactcatg
    32041 attcagctct tcttcagtcg atttcatgat gacatctcgt gcgtcctctt ttgataaacc
    32101 agcaattttt tctagttctt tttgttgatc ttcaatcatt ttttcaacgt ctttttctcg
    32161 ctcttcaatt aattgctgtt ttgaactaag tgcttcttct ttactttcaa gtgagttctc
    32221 gcgtttttct aacgagtcat ctttacgatt cagcgtttgt tcgcgttgaa tcaaacgatt
    32281 ctcttgagat tttaattcta acttactttc tctcagctca ctttcaactt cagaacggta
    32341 tttctgattt tcttccttag cttctaacaa tgcttctttt tttagagttt ctgcttcttt
    32401 ttttgcacta tcgatgattc cagcagctga tgattgtgca ccagctattt ctttctcgtg
    32461 acgtgacttt gcgaccataa accctaaacc aagaccgaca attaaaccga tgatagcgag
    32521 gagaatgttg aataccataa aatccacctc catactatct ttttcatttt tatcatctat
    32581 acagcttcta tgataaatta ttctaaactc gtcaatatgc atcttcgcat atatgtgtac
    32641 aaaaaacatg cacaacttta ttctaatgtt tgtaccccgt agtgtcaact ttaaataaac
    32701 aaagaaacgg ctttctactt attcattaaa cgatggtttt taaaataaat gacactagtt
    32761 tcatataggt aaacctttaa attgagaaac ttttctatta aattattctt tgtagatgaa
    32821 cagatatttt tattttatgt acccactcgt ataattaaat aactcacgat ttctttattc
    32881 aataaaaaaa cggcaggtca aggagagtac ctactcttca gaccagccgt ttttgtttat
    32941 cataattttt attcttctac taaaggcaat tcttcttgtc ctttttcatc ttctgggaca
    33001 gcgacttctt caccaatacc ataagctgct ctaactagag cagaaacttc tgccatcatt
    33061 tcaggatgat tagccatata gattttagcg ttttcccgtc cttgaccaat ccgatcttct
    33121 ttataagaat accaagcacc gcttttatca acaatgtctt tttctactgc catgtctaga
    33181 agttctcctt cttgtgagat ccctaaacca tacatgacat ctacttcagc gattttgaaa
    33241 ggaggtgcta ctttgttttt tacgactttg atctttgtac ggttaccaac aatatctgta
    33301 ccttgtttca attgttctgc acgacgtact tccaaacgga tcgttgcgta gaatttcaat
    33361 gcgcgtcctc caggagtgat ttctggattc gcaaacatca cgcctacttt ttcacggatt
    33421 tggttgatga aaatagcgat tgtttttgtt ttgttgatcg aaccagagag tttacgcaat
    33481 gcttgagaca tcaaacgtgc ttgtaacccg acatgtgagt cacccatttc gccgtcgatt
    33541 tctgctcgtg gaactaaagc agcaactgaa tcgactacta cgatatctac agccccactt
    33601 gatactaaag catcagcgat ctctagacct tgttctcctg tgtcaggctg tgaaagaagt
    33661 agttcatcga tattcacacc taatttttgt gcatattgcg gatctaacgc atgctcagca
    33721 tcaatgaaag cggccgttcc gccatttttt tgtacttctg caatagcgtg tagtgcaact
    33781 gttgttttac ctgaactttc aggtccatat acttcgatga tacgtccacg ggggtaaccg
    33841 ccgacaccta aagcgacatc taacgcaaga gagccacttg gaattgtaga gatttgttgg
    33901 tcgatttttt cgcccaattt cataatagag cctttaccat agctcttttc aatttttttc
    33961 agtgcagcat ctaaggctgc tttacgatca tctgccaata cgataatcct ccttatattt
    34021 acaaatcaga gcaacatttc attatcctta tcatatcttt tttagtgaaa aaaagcaaga
    34081 aaaaaccgaa caaatattcg catttatcat tttcttaaaa gctgttttct caaaagatca
    34141 aatccttgca taaccgcatt atatctgata gcagcacgat ctctggcaaa gtgatatttt
    34201 tccgaaacca cgccgtcttt agatgaaata gcgatccata ctgttcctgc agattgacct
    34261 tctaacgtgt ccggtccagc cactccggtg aaagctacac tgtaatctgt tcttgctatc
    34321 tcacgtgctt gaatggccat ctgctccaca cattctttgc tgactgttcc atattctgct
    34381 aatagctccg gatcaatctc cagaaaacca gcttttgttt ggagcgagta ggtaacgaat
    34441 ccgcctggaa atacttcaga tacaccagca atgtcgccta atgcggcttg gaaggcgccg
    34501 gctgtcaaac tttccgctgc tgttacagtc tgcttgtttt ctttcagtag ttccactacg
    34561 acttttgcta gcgaattatc atcgccgtat ccataaaaat attcccctac tcgttcttgg
    34621 attttttctt ctaatgctaa aagagcttga ttccctgcct gaacatcatt tgttttcact
    34681 gtcagtcgta atgtcacttc atttggtttg gcatatggtg cgatcgttgg attgatctgt
    34741 gtctcaatca gatcttttag ctcagtgacc agtcgcgatt caccaatccc ataaaagcgc
    34801 aaaactttag aaattagttt ttcttcagaa ggaaaacgtt cctctagtaa aggacgtact
    34861 tgttccacaa acattggttt caactcatta ggcggaccgg gaagaaggat atacgcatgt
    34921 gtatctgttt gataaaaagt ccctaaagca agccctgtac gatttggtag aggaaccccg
    34981 ccttcgataa tctgggactg ttgcagattg tttttggtca tttttcgatg ggttgtttca
    35041 aaatatgcca ataatttttt gtatccttcc gtattttgta tgagagactt accgaggtga
    35101 gccgcagttg cttcttttgt caggtcatct tctgtcggtc ctaatccgcc acataagagt
    35161 atgagttcac ttcttgtttc agctagagca atcaactctt ctaaacgctg ctgattatct
    35221 cctacaactg tttgataata aacttcgata cctagatcag ctagctgttc tgacaagaaa
    35281 gtggcattcg tattcacgac ttgtcctagt aaaagttctg ttccgacagc tattatttct
    35341 gctttcatta cttcccgctc ccttatatag tatacgctgt atttttatat ttttttattt
    35401 aaaaagaggg caaggagctg tgacaaaaat cttgtcacag ctccttgttc caaataaaca
    35461 gtgttctcct gccaaaacct cggtcataag tcacaatata cataaaacat gaaaagctgt
    35521 tttttgaaat tatcccttgt gactcagagt tagacggcag gctcacaacc tctccactaa
    35581 acgatactta cattgatcct ttgaacacac cgctgttttt gatgaagtaa tccacacctg
    35641 aatagatagt aaagatcaga caagcataaa gcatgatttg atctactgga ataccgatca
    35701 ggctgaatgg gatattattg ataaacagca agataatagc aaccatttgt gtagccgtct
    35761 tgacttttcc cggccaagct gcagccatga cttctccatg ttcgacaagt agtaaacgca
    35821 agcctgtgac ggctaattca cgacagacaa tgatcgctac tacccaacca ggggcctttc
    35881 cttggtcaac caacatgata aaagcagtca taactagcat tttatctgcc aacgggtccg
    35941 caaacttgcc aaaattagtg acaaggcctc gcgcacgggc aatttttcca tctaaccagt
    36001 ctgtgatact agctaaagca aaaataactg cacctactag ctgtgtgaca gccagtgtgg
    36061 tgtctcctac gttaaattgt ccccaatcta aaggtaaacc tacaaccaaa atgaaaatcg
    36121 ggatcataca aattctaagt acagttaatt tatttggtaa attcacacta tcgactcctt
    36181 tcttccctat tctacgtgac tatcagcgaa gtttccaccg atattattta cttttccaaa
    36241 tgaatttatt caaaggagta tttttattga aattgatctt tttgaattaa cgaagaagga
    36301 ccgcgacaca gatttgtcac gatccttctt ttttatacct acaagtaaac agatgcgtaa
    36361 actaatttat acgatcattc tgcgtattga agagtaaggt tcacattttt tggactctga
    36421 ttgttttccc ctggattaac tggtacggat tctccattcg ctttgatcgt tgcattgctt
    36481 gctgcaccaa tcgtgatggt cgcatttgtt gctgtttccg gtaacacggt actctgtgtt
    36541 tcatttgctg ccagtgtccc ttgataaaca taggcattgt ctactagcac acctaaccat
    36601 acacgattga cagcagtaaa ttcaagggtt acaggcttgg tagcgtctgt gatatttact
    36661 gcgatggcac tgccagaatc ttctcctgta gctattgcca ttttcttttc ttcacttgat
    36721 tttgttgttg attctactgt agaagactct ttggtctgcg tgctttgctc tgaggaagag
    36781 gactcctcga ctgatccttc tactttaagc gaagaagctg tgtctccgat tattggatca
    36841 gcttgacgat cttgccaagt catatagccc acgacaacga caatcgccaa agcaaccaat
    36901 cctaataaaa tgacaggaag acttgtccaa aagcgacttg gatgcgattc ttcctgatgc
    36961 attgcttttc tagaagtact tactgtttca ggctgcggac gttcagggac cgatcctgtc
    37021 aatttgcttt tcccatcaaa aacatcgact aaatggtctc catcttctcc tacagcttca
    37081 gcgtattgcc gaataaatgc ccgcacgtaa aatttaccag gcaattggtc aaaatcatca
    37141 ttgtcgattg cttccaaata acgtttttga atttttgtaa tctgctgtaa ttcatctaaa
    37201 gatatttttt tattcaggcg tgcttgccgt aatttttctc cgatggtttc aaaagccact
    37261 cgttcatctt ctccagtctg tttgattctc cgaagcatct gctgttgcaa cgaaagacgt
    37321 tatgtttggg tgctgtgaca aaagtcatgt cacagctcct tttttccgaa taaacattgt
    37381 tctcctgcca actcctcggt cacaagtcac aatatccaat aaatataaga agctgttttt
    37441 tgaacttgtt ccttgtgact cggagttaaa cggcaggctc acgacctctt tcttaaacgg
    37501 tgttcaatca gctgttcctc ggtattagct caaaaaccgg caaaatataa ggagatattt
    37561 ttccgtatgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttccttaaac
    37621 ggtgttcaaa agcttgcatc tgcggtaata agtccaacca agttaaaaac attaagaagc
    37681 tgtttttact tggctgttct tattacttga tgcagggcag ctttttcaca acctcttggt
    37741 acacggtgtt ccagaagtag cttcttcggc aataagccga aatatcacaa aaatttgaga
    37801 agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctctt
    37861 tgattacttc gtttgatttt attttaatat aagttgatta aaagagagca tgatttgttt
    37921 tattttctca ctaatttttc caacacaact aagaatacca taaaagctga ttttggagga
    37981 ggttcctact acaagtctaa aacgaaattt aagacttttc ctcataaaat actggtttat
    38041 tttggtctca tgtaaaactc gctgaaggcc tcttcactta taaatgcttc cccagctgcc
    38101 agtacgtctt tcatttggat cgaatcgatg atttcaggaa gatcaaataa agttcgatcg
    38161 ccaaataaac tttgcgtaaa ttgattggca atatattcga tcgaatttaa tgattggaaa
    38221 tattgtccaa gcattttctt cttcagcaaa tcgagatttg tttctgacac ttccggatca
    38281 tcagcgaatc caagaatgat ttctttgact ttttctgctg ctttctctgg ttcgtccgta
    38341 tctccgctga agtccgcaaa gtgaaattca cgatccaagc tgaattcaaa accaaagctg
    38401 tcatcgataa ttccttgatt gtacatcgct agatagtttc gagaagtatt gcctaaaatc
    38461 atttggaaca acagattgat tgctgtttta tagcgtaaca gttctgttcc ttcttgcggc
    38521 aatgtgtcta atcctttaat accaagtaca aatttatctc gtgtaatggc tgcttccaat
    38581 gcactttgtt tgatgatttc ttttgtgttt tcagggaaat aacgtacaat ttcttgcttt
    38641 ggagggaaat tttttgcttc ttggttttcc cgaataagtt tcatcaattt ctcaggctcc
    38701 atcttaccaa ctacaaataa aaccatattg cttggctgat aaaacgtccg atagcacgta
    38761 tacaagtctt gagctgtgat tttgtcgata ctttcaaccg tgcccgcaat atcgatatgc
    38821 aatgggtgtg taggatacaa gttatttagt atgccaaaaa acatccgcca gttagggtcg
    38881 tcttcataca tttggatttc ctgaccgatg attccttttt ccttgtttac agtttcttct
    38941 gtgaagtaag gtgcttgtac aaaatcgatc agagtcgtca gattcttttc tacctgatcc
    39001 gtcgtagaaa acagatagct ggttttcgta aaacttgtga aagcattggc ggaagctcct
    39061 tgtttcccaa ataattgaaa gacatcacca tcttcttttt caaacaactt atgttctaaa
    39121 aaatgggcaa ttccatctgg aactttcacc ttttcttttt caccataggg aataaattcg
    39181 ttatcgatcg aaccataatt cgtactgaac aaaccatatg tcttgtgata gtcattttta
    39241 ggcaacaaat acacggtcaa accgtttggc agtacttcat gataaagtgt ttcattgatt
    39301 tgctcatatt ctgttttatt catttgcatt tcctcctgct aaaaagaaga ttgcctgcaa
    39361 ttcgatttgc tcagctactt gttgtatctc tttgatcgtt acgctctcca tacgtttcaa
    39421 ccattcttca tcagataatt ttgtttccgg cagccagctg tccaaaaaag aagcttcgat
    39481 tgccgcttgc ggagagtcta gagaaagcag atattggttt ctcagcattg cttttgtctg
    39541 agacagttcc aactctgtta tttcaccatt tcgcaaactt tctaattgct cgtgaatcag
    39601 atgaagtact tgatttcggt tcttttcatc gataccggtt tggacactca tgaatcctcg
    39661 gaacgtatcc acgctactag aggcataata agctagactt tctttctctc tgacattcat
    39721 gaaaagttta gagtgaggaa aaccgccaaa taaaccgtta aagaccatta gcgcaaaacg
    39781 ttctggttca tcgtaataca catttgtctg ataagctagg ttcagctttg cttgaacgat
    39841 tggctcacgt acttgtcgtt ccttcacgat attcacttga ggttgggtat aaaacagatc
    39901 tggataaact gtttcccgcg actcaaatgg taattgccca actgcttcag ctgctttgtc
    39961 cggatcaaca tcgccgacaa caaaaatgtc gatttgatca tttgccatca tttcttgata
    40021 gtaggacgct agcgaactag cagtgatctt gtccaaatct tcgactacgc ccaaccctgg
    40081 gatcttttgt tccgggctgt tttcaaaata aagttcttgc aaggcatagg aagcaaaagt
    40141 ttgtttatct tccttcaaac tttccaaata tagacgcaga ttgttctttt ccaagtcaaa
    40201 cgtcaactga tcaaactgat gatttttaat atttggataa aataaaacct cttttaaaaa
    40261 atctaccgct tgagaaaaaa ggtttggatc attgacatat tcaccattca caaaactgat
    40321 ccctgcgttt agccaatgta aatttccttt ttttctaact gataatccaa agcttgcgcc
    40381 ataaagctct gcaagttgcg cacttaactt tgtctgatcc gaataattca aactatttgt
    40441 ttccaacatg ctgctgagaa gtgaacgttt cgtgatgact tcttgttgca gacgcgcagt
    40501 aaaacggata aaaatccgga tcgttttgta ttgttttgtt ggtaagacat gaagattcac
    40561 gccttctgcc aattgataca ccatatttat atgctccttt tcacagttga ttgcccgctt
    40621 actcttttaa tgggtcaacc tgcctttttt acttattcct ctgcttgaaa tttataagtt
    40681 caggcaattc gccgtttttt ctaaatgatg ttacacttta tagtatagca tagatgtttt
    40741 ttatcaaaag aaacagacgg ctaaaagcgt atattgtttt tttatttttt atttttcgat
    40801 atactaaaga caaataaaag aagggagggt cattcatgat caaagaattc aaagagttca
    40861 tcatgcgtgg agatgtcttg gatcttgctg tcggcgttgt tattggtagt gcgtttacaa
    40921 gcatcgtaaa ccaagtggtg caaggcttga tcacaccact aataggttgg tttgtcgcaa
    40981 tgatcacagg tacttctgat ttagaaggag cactttcaat tcttgactgg tcgccgacaa
    41041 aaggcgtcac atttgcgttt ggtaacgtta ttagtgctat cattacattt ctgatcactg
    41101 gttttgtctt attcttagtt gtcaaagcgg caaaccgtgc gaagaattta cgctccaaag
    41161 aagagcaagt ggaagtggaa gaaactccta ctgcagaaga ttatcttagt gacatacgcg
    41221 atcttcttgc caaacaagtg gcagaagaca atacacatcc agtagagcaa acaaatcttt
    41281 ccgatacttc taaataataa agaatctaaa aaaaagaaag ccgattttca gtgtcggctt
    41341 tcttttttta gattcttaac ttgctacttg ttcctttttc atgtcgatat gtggaatatc
    41401 atcttccaaa tacacatctg agattgcctt gaatccaaac gactcataaa aagcttgtag
    41461 gtattcttgt gcgccgatca cgattggttt ttctggaaaa tttttctgga tcatgttaat
    41521 cgtttcttga acaatctttc ttcctaaacc atctttacgg ttgtcctgat gaacaaggac
    41581 acgtccaaaa tggatactct ccgcttcttc atatactcgc gtgtaggcaa ggatcttccc
    41641 atcagtatct tgaaaaaacg tatgccaagc ttctccatca atgtcatcaa cttcttgata
    41701 aggacactgt tgttccacaa caaaaactgc gattcgttct ttgacgatcg cgtaaaactc
    41761 tgctgttgac aattcttcaa atttctttac ctgatattcc actaagcaaa cgccccctgc
    41821 ttttttattc ttagcatacc gattcgcaca caaaaaagca aacgaacctt acgaatctca
    41881 ggctcgtttg ctttttaaac tgcttcagtt aaaaattcga tctcaactgt ccgagtaagt
    41941 acatctgaat agctgtaaga tactcgttca aaagaatttt cttcaggatc caaatcaaca
    42001 acgaatactg aaggataggt ttctgttaaa acgccgcggc gttctgtttg acgctttctt
    42061 cctgtctgag caaccaacat gattgggctg ccgatacgac cttctaaatc tttcttgatt
    42121 gaagctaaag ttgttggcat acagttcacc tctactatca actataccac atatttttaa
    42181 aattttcaag tttaccataa aaaaaaaaga aatgcaataa aaataaataa gaagctcatt
    42241 aagatttttc taaattattc ctcattataa aatttcaagt gtttttttat tttgagtttt
    42301 actctcccgt aagcgctcat tacttgcttg tgatccattg ctaaacattc agccatgtct
    42361 acaaaatcaa cgccttgcat ataataatga aaaattttag cttctatttt tgaaaactgg
    42421 atttcgcaat tttcgattgc ttcttttaat aaaagatgct gaattgctaa aggttcttgc
    42481 aatgtgatct ctgctaaaaa gtctggtcct tctgtctcta ttttttggtc gattgagact
    42541 tcatcttttt gatcacgacg cttcagcgcg ttctgcttcc tcaacagaga atagatttga
    42601 ttgcttataa cgcttttata ataacttcca aagctaacac ctttgctttt ttcgtaagtt
    42661 ctcaatgcct gatataaagc gatccttgct tcttgttccc aatcctcaga aacataattt
    42721 cttaaataat aggtgttttt caaacgtagt aaaagtggaa aatatttttg atacagggca
    42781 gcaaatgctt cttcgtcacc attacgtgca gcgcgcaata actcctcaga cataaaataa
    42841 ttcctccata aattcgttta tggaggaatc ttatcatcaa tgattcatcg aactgattag
    42901 aacgatatgt tatttatttt ttctcattaa atcgtccaat ttttgggaca atttttttaa
    42961 ctgctcttca ttccatggag aattacgtcg gaaatcaata aaatgcatat ctttattttc
    43021 ttgggctatt gttttttccg ttttttttat cgtcttatat aattcataag cagaagttcg
    43081 taatgctcct tgggaaaaga tcatccactg ttctgctaaa tcgcttgtcg ccactgtcac
    43141 ttgtgtcaaa cggtcattca gctgaccagc gattctctcg atataactgt cagccgtttc
    43201 gtcttctttc gtaaaaacaa catccaactg gtatttctta taattttgtt ggatgcccgg
    43261 aactagttgg gcatcgaaga caacgatgac ttccattcct tcatatttcg cataatttga
    43321 taaacgatgt aaaagagctt ctcttgcatc cgccattttg tcctgctttt taagctggac
    43381 aagttccggc cacgaaccaa tcatgttgta gccgtctaca atcaacagct gttttttcat
    43441 ggtctactcc ccctgacgtt gggaaaaagc ctgatacata agcaaaccag cagcaacact
    43501 ggcatttaag ctctggacgt gcccggtcat cgggatcgtc agcaattcat ccatttcttt
    43561 tttcaatcct ggactcattc ctttgccttc attaccaatg actaaagcaa ttgatccttt
    43621 ggcattccat ttttggaagt ttgtgccggt catatctgtg ccaaataccc aaaatcctgt
    43681 ttctttgagc tgttgcacag cttgtaccaa attcgtcacg cgtgcaacag ggacatgttc
    43741 gactgcccca gtggaagtct ttgttacgat aggtgtgatt ccaactgctc ggtgttttgg
    43801 tatgatcaca ccatcgacgc cacttgcgtc tgctgtccgt aaaatcgacc caaaattgtg
    43861 tggatcttcg atgctatcca aaatcaaaaa gaaggggtct tcttttttct cttttgttcg
    43921 ttcaatcagt tggtcaaggg ataaatattc ataaggtgta atcgcaagaa cgactccttg
    43981 atgcacgccg tgatcgctca aagtttctaa tttttgttta ggaacccatt tgactggcac
    44041 agcttgttca cgtgcgattt ctttcagttc ttctactttt tctcctcgtg aatcttcttg
    44101 aagaaagagt ttattccctc gcccttgttg tagcgcttct actgcggcat ggtgcccaaa
    44161 gacgaaatta tcttctaatg cttcttcatt ttgatcagaa actgactgat ttcgtccagt
    44221 ttgcttttta tttgaatatc tattttgttt tttgtcttca aatccacgtt tcgattgatg
    44281 tttgtttgtt ctttctttac ccattttttt caccaacttt ctgaatacac cagcggatca
    44341 attcttccat ccgctcggtt tgttttgtca aatgcagata gcccatgagc gcttcgaacc
    44401 ctgttgcgac ccgatacgtc gtaacatcag cattttttgc gctcgtatgg cttttagcat
    44461 ttcttcctct gcgataataa agttcttctt cttctgtcaa aagatgttct tccagcattt
    44521 gctgaatcaa aaaggcttgt gcattcgcag aaacataatt cgttgccgca cgatgcagct
    44581 tattgggttt tgtttctcct tgatcaatca ggtggtcacg aacatagatt tcgtaaatcg
    44641 catctccaac ataagccaag gctaaaccgt tcaattgtgt gtaatctctc atgcttctct
    44701 tctccatcgg gttccttgcg gtgtatcttc taataagatc cctttttctt tcaacaaatc
    44761 acgaatttcg tcgcttcgga caaaatcacg attgcttctt gcttgatttc gttcttcgat
    44821 caattgttca atctcttcat ctaataactc ttctgctgta aataaaatac cgaagatcga
    44881 aagccattgc gagaataaag cttctccttg tgccaagaca gcggcagaaa ctttcggctg
    44941 gtctagataa gtgttcagcc acttagcaag ctcatagacc actgtgatcc catttgctgc
    45001 attaaaatca tcgtccattt cttcgatgaa tcgctgttct aaattttcta acaaagcgat
    45061 tttatccgca tcatctgcca aattctctga ggcagtttct tggcggaatt tcaaattgtc
    45121 aaacgtattc ttcagtcgtt gtaaattggt ggctgcttct ttcatcgtcg tttcactgta
    45181 gcggatcgga cgacggtatt gtgtggtcgc catgaagaaa cgcaagactt gtggatcaac
    45241 gtttttgatc atttcatgta cagtgatgaa attccctaga gatttactca ttttttcatc
    45301 gtcttcgcca atcgttacat agccattgtg catccagtaa ttagcgaatt ttttccctgt
    45361 tttcgcttca ctttgtgcga tttcattttc atgatgagga aattctaaat cttgcccgcc
    45421 accatggata tcgatcgttt ctcctaaatg ttttgttgcc ataacggaac attcaatatg
    45481 ccaaccggga cgtcctttgc cccatggtga atcccaagaa atctcgccag gttttgcttg
    45541 tttccataaa gcgaaatcta atgggtcttc tttttgctgc tgttcttccc ctgtacgctg
    45601 acttgctccg acttccagtt catcgatgga ttggtcactt aatttcccat aattttcaaa
    45661 tttacgggta cggtaataca catcacctga agattcataa gcatagcctt tttcaaccaa
    45721 cacttgaata aatgctaaaa tatctggcat atgatccatg actcttggat gcagtgtagc
    45781 aggttcgaca ttcaacgctt tcgtatcttc ttcaaatgct cggatgaaac gatcagcaac
    45841 ttctggtgct gtaataccta attcgtttgc tgtacggatg attttatcgt ctacatccgt
    45901 gaaattcgat acgaaatcga ctttatagcc tcgatattca aaatagcgtc gaattgtatc
    45961 gaaagcaatg gtgctacgag catttccgat atggatataa ttatacacag tcggtccgca
    46021 gacatacata ccgacatgtc ccttttgtat agggatgaat tcttcttttt ttctggtcaa
    46081 tgtattgtag atttttatca ttctgatctc ctcacttttt ctcctttgat tcgaacgact
    46141 tttgctggta ctcctaccgc tgtagcgtcg tctggaacat ctttcaacac aacggcacct
    46201 gcaccgattt tggcgttttt tccgatcgtt accggtccta atatctgcgc attcgcagag
    46261 agcatggctc cttgtttaac agttgggtgt cttttacccg tttcttttcc tgtgccgcct
    46321 aatgttacgc catggaataa aatcacatcg tcctcgattt ctgcggtttc gccaatgacg
    46381 attcccattc catggtcgat gaacacgcca gttccaatcg ttgctcctgg atggatctca
    46441 acaccagtta ggaaacgcca gaattgagca ttgatcttcg ccaataaaaa gagccggtgc
    46501 tgatataaaa aatgagagaa acgatgccaa aacaaggcat gaaaaccagg gtatgtcaaa
    46561 acgatctcag ctgtcgaacg tgcagccgga tcgtttctct ttgctgcatc aatcgctcgg
    46621 gtaaaccaac tcatgacttt ccactccttt cgtaagaatt ttatgtagga aatcatcttc
    46681 ttataaagaa aagaagcgcc ttttaaataa gtttccttat caaaagacgc tttccgcgtg
    46741 gttccacttt tcttcataat caatgattat ctctgacagc taacgcctgt tatacgttct
    46801 tggctacttt gtttcctata aaacagtttt tcctgttttc tgtgtttcgc caagaccact
    46861 cccagaggca tttcggcaac cattcattga taccttgcac cacccggtat ctctcttgta
    46921 atgatcagct gcttactttt ttctgttcat cgtgtttagt tcagtacttg atttaaatgt
    46981 gctaatgatt tttcacgacc aagcaattcg atcgtttcag gcaattctgg accatgcatt
    47041 tgtcctgata cagctacacg gattggcatg aaaagatttt ttccttttac gccagtttct
    47101 ttttgaacag ctttgatggc agctttgaca gttggagcat ctactacttc catttgttct
    47161 agctgtgctt tgaatgcgtg gagaacagta ggaactgttt caccagcaag tacttctttt
    47221 gctgcttcat tcaatacagg atgttcattg aaaaatagcg tagacagatc aacgatttct
    47281 gcagcatagc tcatttgagg ttgatacagg ctaacgatct tcttcaccca ttcgattgtc
    47341 ttttcatctg ggttttctgc tacacgtcca tcagcgatta gataaggcag acacatgtca
    47401 gtaaatgcat ctagatccat tgctttgatg taatggttgt ttacccattg cagttttttc
    47461 gcatcgaaag ctgcgggtga tttacttagg cgttttggat cgaacatttt gatcaattct
    47521 tggtgttcga agatttcgtc ttcgcccaca ggtgaccagc ccaataatgc aataaagttg
    47581 aacattgcat ctggtagata gcctaattcg cgatattgtt cgataaattg caagattgat
    47641 tcgtcacgtt tgcttagttt tttacctgtt tcactgttga tgatcaatgt catatgtccg
    47701 aaggttggtg gtgtccagcc aaatgcttca tagatcatca gttgttttgg tgtattggca
    47761 atatggtcat ctccacgtaa aacgtgagtg attttcatca aatgatcatc gaccgcaaca
    47821 gcaaaattat aagttggcat accatcgcgt ttttggatga caaagtcgcc accgatatta
    47881 tcggattcga acgaaatagc gcctttcacc atatctgtaa atgcgtattc tgtattgcga
    47941 ggcacacgga aacgtacaac agattctaga ccttgtgctt ctttttctgc ttgttcttca
    48001 ggtgtcaagt tcgcacatgt tcctgcgtaa tgaggcattt ctcctcttgc gcgttgtgct
    48061 tcccgttctg cttctaattc ttcttctgtg caatagcatt tgtatgctcg attgctagca
    48121 agcagctgat caatcagcgg ttgatagatt tctttacgtt cagattgacg gtaagggccg
    48181 tattctccag gattttcagg agattcatcc caattcattc ccaaccaagc aaggttttcc
    48241 aactggcttt tttcgccatc ttcgatatta cgtttttggt cagtatcctc aatacgaata
    48301 ataaagtctc catcattgtg gcgggcaaac aaataattga ataatgcagt tcttgcattt
    48361 ccaatatgca ggtgcccagt tggacttggt gcatagcgta cgcgtacttt cgtcattttc
    48421 ttcctcttct ttcaaactag actttgacta acagcaaaaa ttccgtcagt tacggcttct
    48481 caaatatcat ttatttaaag ccactcagca caaattttac cttttctaag gtaaatagta
    48541 aagccaactg gttgaatttc cttaccagtt ggctgaattt cttttttatt ttgattgttt
    48601 agtctttttc tctgtctctt ttttattgtc gattcctcgt cctgcatgtg caggtttagc
    48661 aaagatcatg cgtccagcag ctgtttgaag ggcacttgtt acaacgactt cgatacgttc
    48721 attcatgtaa tgctgtccat cttctacgac aaccatcgtg ccgtcatcaa ggtaggctac
    48781 tccttgttgg cgttctgtcc cggcttttac tactagaaca ttcatcgttt caccagggat
    48841 gacgactggt tttacggcat ttgccaacgc attgatatta agaacaggta cattttggaa
    48901 ttcagagact ttattcaagt tatagtcatt ggttacaatg acaccatcta aaagcttagc
    48961 caatttgatc aatttgctgt ctacttcact gatatcttca aagtctccat cgtacatttc
    49021 gacagatata ccctcttctt tttgcaaggc atttaggata tctaatccgc gacgaccacg
    49081 cacacgtttc aaactatccc ctgagtccgc gatgtattgt agttcgtaaa gaacaaaatt
    49141 agggatcatc aaggttcctt ctaagaatcc agtctttgta atatcataaa tccgaccgtc
    49201 aatgatcacg cttgtatcca agattttata tttatggaaa ttttctcctg attttcgttc
    49261 taacagttga ctctcaactt gactagctgt ttcttgctcg atttttttgc ttcgtgaacc
    49321 aaaaactttc ttccattctt cgattcgtgt cgttcccatt ctcaatccca gatagcctaa
    49381 gatgatcatc agtaaaatcg gcaatacact attgacgaat ggaatttcca tattgtaaag
    49441 aggggtcgag atcacaacac ctaaagtcaa tccgatgatc gcaccgatcg caccgaagag
    49501 aagataagtc aagctgacct cacttagttt ttgttctact tgtttgataa tagctgagat
    49561 atgtttggcc aacagcaaag ataataaaaa gaaaataagt gcgcctaaca ggctgttggt
    49621 aaatgtatta ttcagccatc cgtttgactg ttgaccgatt gcctgccaag ctgagggtaa
    49681 aaatgaaata cccaagcttg cgccgacaat gatcatcagc aaagtaatga cacgtttttg
    49741 catcgttttt cctccgtttc gacaatcatt tattagttag cggaacacac gcttcaacgt
    49801 ttcagccaaa gttgctactc caactatctc gattcctttt ggcgcttccc agttacctaa
    49861 gttgttttta ggtaggtata cttttgtgaa gccgagtttt tggacttctt tgactcgttg
    49921 ttcaatatga ctgacacgtc tgatttctcc agtcagtcca atttcaccga tgaaacattc
    49981 cgttggctgt gttccttttt ctttatagct tgaagcaata ctgacagcga tagctaaatc
    50041 aatcgctggt tcatttaatt ttaccccacc tgcagctttt aagtaagcat cttgattctg
    50101 aagaagcaac ccagctcttt tttccagcac tgccatgatc agagataccc gattgaaatc
    50161 aagacctgtt gttgttcgtt tggcatttcc gaacatagta ggtgtgacta atgcttggat
    50221 ctctaccaaa attggacgtg tcccttccat tgccacaacg atagcagagc cagttgcacc
    50281 gtctaggcgt tcttctaaga aaatctgtga aggattagcg acttcttcca atccatgctc
    50341 atgcatttca aaaatcccaa tctcattcgt tgagccaaat cggtttttta ctgctcgcaa
    50401 aatccgaaaa ctatgatgtt gttcaccttc aaaatataac accgtatcca ccatatgttc
    50461 taacattctt gggccggcga tcgagccttc tttggtaaca tgaccgacga taaaaatggc
    50521 tatcccattt gttttagcgg ttttgagtag ttctgcagtg gtttctctta cttggctgac
    50581 acttcctgcc acacttgtga tgtctggctg cgtcatcgtt tggatggaat caatgataac
    50641 ataatcaggg gagatatgtt cgatggcacg actgatttcg ttcatatctg tttcagcgta
    50701 tagataaaat tctgtgttga tacttccgag gcgttccgcg cgtaatttta tctgctctgc
    50761 gctttcttcg ccggatacat ataagacttt tccgcctatg gctgctaatt gctgagaaac
    50821 ttgtaacagc aaagtggatt tcccgatacc tggatcgcca ccaatcaaga ccatagaccc
    50881 tggcaccaca cctccgccta acacacggtt cagttcttct aattttgttt tgatccgagg
    50941 ttctttttta gggacgacgt ctgcgatttt tgttggtttt gctttttctc ctgtcaaact
    51001 tgtccgagtt ctgcggtccg ttgtatcttg ttcgatttct tcgaccatcg tattccattt
    51061 tccgcaattt gggcagcgtc ccaagaactt aggcgacaca tacccgcaat tttggcaaac
    51121 aaattgtact tttgattttt ttgccattgt accgcctcct tttatcctaa tcatgagaca
    51181 ttaaatcaag cttaagtatc tttaacgttt tatttactgg aagaaccaaa tccgccttga
    51241 cgttgagcag tcgtttgatc ttgatctgct tttaaaaacg gcaaaaagat cccttggccg
    51301 atacgctcgc cttttttgat cactacatcc ttgaaaccaa agttggtgaa ttggaacatg
    51361 atatggcctt catttgcctc attattgtaa taatcactgt caatcacacc tactccgttt
    51421 gtcaacacta aaaaccgctt caacggattg cttgaacggt tagcaagctg caaaaattca
    51481 tcttctccca tgtaagcttt gattccagta ggcacgaggg ttggtttcaa ttgtttttca
    51541 ttttctgata catctttttt caatccatct gcgactgttt ccaacccctt tttccagcct
    51601 gctttccaga tacttggaat cacgacctct tctgctgctt caaaatcata gccagcagca
    51661 tgatgtgtag cgcgctgcgg tagattgata ttttgatctt tatatttaga aataacttca
    51721 aatcctcttt ttctcaccgg ttttcctccc taaataaact actcctattc taacatatcc
    51781 gaggctgtaa aagactttct ttacaaattc ttcaagaaat tctgctatct taaagtaaac
    51841 cattaattcg ttctttttcc aaacgactgc aagttgtaca aaaaaactat tttttacatg
    51901 actttactcg tcaaaacaag tagaatagaa cgaaattaat gagtggatat ttgattgagg
    51961 tgtcacatgc gtaaagaaat taaaaatgca aaacgaatta ttgtcaaagt aggaacaagt
    52021 acattgattt atcccaatgg aaatatcaat cttgcggcaa tcgatcagtt agcttttgtc
    52081 ctttctgatc ttagaaatcg gggaaaggaa gtgatcttag tctcatcagg agctgtaggc
    52141 gttggtatgc ataaattaca tttgtcagaa cgtccgactt tgattcctga acagcaagct
    52201 gcggcggctg ttggtcaagc cgaactgatg aatatctata atcaaagatt cagtgtatat
    52261 aatcaacaga cggcacagat cctcgtgaca agagacgtgg tagattatcc tgaaagtcgt
    52321 aaaaatgtga ggaatacatt ggaacagctt ttgattatgg acacgatccc tatcgtgaac
    52381 gaaaacgata ctgtagcagt ggatgaatta gatcatctga caaaatttgg cgataatgat
    52441 cagctgtcag ccatcatggc aaaacttgtc gcggcagacc ttttgatcgt cctttctgat
    52501 atcgacgggt tttattcaga taatccgact acaaatcccg aggctcagat gtatcgctat
    52561 atcgataaaa tcgatgaaaa gattctggca caagcaggtg gtgctggtag cacatttgga
    52621 acaggcggta tgcagagtaa attaaaagcc gcttctagga tatttgaagc aaacagcgca
    52681 atgattttag caaatggaaa aaatccggca attatttttg atattcttgc aggaaaagaa
    52741 gtaggtactt tatttaagga ggacttgaaa tgactgactt gatgcaatta ggacaacagg
    52801 caaaagatag cgcgcaacaa ctagcactta tgcctacaaa ccgcaaaaat gagctacttg
    52861 ttcaaatggc acaaaccatc aaaaagaatc aacaagcgat cattgaagca aatcataaag
    52921 acttagaaaa agcagtcgaa aacaatatca gtgaaacgat gcaagaccgc ttgcagctga
    52981 cacctgagcg aatcaatgcc atggctacag agatcgaaaa aattgcttcg ttagacgatc
    53041 ctattggaaa agtagatgag atgtggacga acaccgacgg attgagaatc ggcaaaaagc
    53101 gcgtgccact aggcgtgatc ggcatcattt acgaatctcg tccaaatgtc acgacagatg
    53161 cagctagtct agcattcaaa tcagggaatg ccgttatcct tcgcggtggc aaagaagcat
    53221 tcttctcgaa tcaattatta gtccaattat tgcagcaagt actggtcagt gctggcgaat
    53281 ctccttatgc catccagttt gtcgatgata cttctcatga aactgcagga cagttgatgc
    53341 gattgacaga atacttagat gtgctgattc ctcgaggcgg agcaaaatta atccaacgag
    53401 tcaaagaaca ggcaacggta cctatcattg aaacaggtac aggaaataat cacgtatata
    53461 tcgataaaga agcacagtta tctatggcag tgaacatcat cgtcaatgca aaagcttccc
    53521 gtccatctgt ctgtaacgcg gcagaaacat tgctgatcca ttctgagatc gcaccttttt
    53581 tcttgccagc tattgaaaaa gaactggtag aacacggggt ctctttacgt gcggatgctc
    53641 gtgcgttaga atatctagag acggctgctt tagcagaaga cacagattgg gatactgaat
    53701 atctagatta tatccttgct gtcaaagtgg tcgattcttt ggatgaagca attgcacata
    53761 tcaatcggca taatactaaa cattcagaaa cgatcgtgac agatagttat gcggctagtc
    53821 aacgtttctt aaatgaagtg gacgcggcgg ctgtctatgt caatgcctcc actcgtttta
    53881 cagacggatc ggtatttggt tttggtgcag agattggtat ctctactcaa aagttgcatg
    53941 cccgtggtcc aatggggtta aatgaattaa cttccacaaa atatatcatt tatggcgatg
    54001 gacaaatccg cgaataatgt ttcgtgtgaa acattattcc cgccttttgc cttttttctg
    54061 taaaggttct atccaactag aaaggtgtca atatgtttga aattttaatg atttttttta
    54121 tttaccttat ttctctgaat atcgctgctt ttctgggagt cggtatttta tcccttttct
    54181 ttcaattcaa aaaacgaagc atagggagtc aacgggaaaa atggtcgcag tattttgaca
    54241 aaatcgggca aaaagggctg gttacacgct tgcacattag ttacatggtt gcattatgct
    54301 tattagcgac aatcaattac tattcttttt tcgatcattc aatcgcttat acgattacgc
    54361 tactgattgc tgggattttt cacttatctt ataaatatca gctgaataaa aaccacttga
    54421 atcggacttt ccgctaaaaa atatccgtac gataagtaga gtgccttgaa gatcggcaaa
    54481 aaactgccgg actttttaga ggctgtgaca aagattttgt cacagcctct ttttccatat
    54541 aaacggcgtt cgcctaccag ctgcctggta aatctgtttc ctcttcgtta agaagcgaaa
    54601 atccgtcaaa tgtttcgcca tcataataac gaagcagctc atccatggac aagttcatcg
    54661 ttttttcttt ttcttcttcc gctgattcag ccagtagctg ttcccgatac acctgataca
    54721 taccagctat tttttgctgc atcgcttttc tagtttcacc ttccgcattg attattggca
    54781 attctttgat atagccacga taaatcaacc gatcatctga aaattcaatg ggtaattcaa
    54841 cgagtgtcaa cccgacttcc tgaaaatatt cttccagcat ctattatccc cccattcttt
    54901 ttcttaaatg aattttagct tacctactga aaattttcca gtttcattat tttgagaaaa
    54961 aaagggaatg tgacaaaaat cgtgtcacat cccctttttc cgaataaatg gtgttcttct
    55021 gccaactcct caatcataag tcacaatatt caacaaacat gagaaactgt tttttgaaat
    55081 tgttccttgt gattcagagt taaacgacag tctcacaacc tcttggtaaa cggcgttcaa
    55141 tcagctgacc ctcggtatta gctcaaaaaa cggtaaaata tgagaagcta tttttccgtt
    55201 ttgtttctct aatacttagg ataaaacgct gattttacaa cctctgttaa acggtgttca
    55261 atcagctgtc cttcggtatt agctcaaaaa acggcaaaat atgaggaact atttttccgt
    55321 ttgtttctct aatactcagg acaaaacgct gattttacaa cctcctgata aacggtgttc
    55381 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagaa gctatttttg
    55441 tttggctgtt cttatgactt gatgcaggac agctttttca caacctctga tttcttattc
    55501 ttcttcattt gtagcaaaat aattacggtg ataaatgata caacatacac tgataagaac
    55561 aatggcacct gcaaaataaa acgcatagct ttcaccaata tgttcaccaa tcacacctgc
    55621 aataaatggt gctaaagctc caccgaaaaa gcggacaaaa ctatagctgg acgaagcaac
    55681 gtttcgttgc agacctggta tttccatagc aatcgtcgtc aacagtgtat tgataattcc
    55741 ttggaaaaat cctgctaaca caatgctgat cggtacaagg actggcatag aagttccagc
    55801 tcctaataac agcaaacaaa taatgaataa gacaaatgct gctaaaattg tccgataagt
    55861 cgtaaacttc cgctcaagag ccggtgcaac gaaaatcgaa gcaatagcca gaagcacacc
    55921 ccagccaaaa aatacaaaac cgacttctaa ttcagaatag ccaatcaata aaaatggcgc
    55981 atacgcaagt aaagtaaaga atccgaaatt atataacaat gcgattagtc caactgaacg
    56041 aagtttatga ttgcttaacg ctttgacacc ttcccaaagg cctacttttc tctttggttt
    56101 ctcagtttca ggtaaaagta ccgctagggc tataaaaccg caaaacatca aaaatgctac
    56161 cccaaaaaat ggataacgcc atgaaccact gcccaagacc ccaccgacta gagggccaac
    56221 agccattccg cagccaagtg ctgcttcata catcatgatt gctttttctg tgttaccaac
    56281 taaaatactg acgatcgttg ctaaagcagt agagacaaat aaagcattcc cgacacccca
    56341 gccggcacgt aacccaatca gcattccgat attcccagat aatccgccaa gaccagcgaa
    56401 tacaacgatg ataaacaatc ctgtcatcaa tgtttttttc aaaccgatcc gactagataa
    56461 gaaacctgta aatagcatca ccacaccagt gactaacatg taactagtaa ataacagggt
    56521 cgtttcagct ggcgtagcgt ttaactgtgc agcaatcgtt tttaagattg ggtctactag
    56581 acccactccc ataaaagcga tcatacaggt agctgctaca actagcgctg aacttttccc
    56641 ctttgtttct tcatttgttt gttcttgtat atctatttga ttctccaata tttccatccc
    56701 tttctctcta ttagcatcat acatgattta ttttacatgt caatatgcac atgtaaaacg
    56761 tggaaacttt actgtgatat aataaagaaa ataagacgag aggtaggatt tttatgaata
    56821 ctttagccta tatattatta tgcgtgcttt tatccaaacc acgttctggg tatgaattga
    56881 agcagctgat cacaattttt tgggaagccc atcacagtca gatctataca acgcttgcca
    56941 agttagacca gcagggttat gtgaccgttt tggacaaaga cgagcactct caaaaaaaga
    57001 tctaccactt gactgatgaa ggacgtgctc ttgtagagga atggatcaaa gaggaaactc
    57061 ccgctcctac gcaaaaagat gaatttctag caaaaatcta tgctttttcg acgctcgata
    57121 aaacaacagc tactggactg ctattcacta gagaacaaca gctgaacaaa atcatgcaaa
    57181 aaaatcaaaa aaaattgaat gacctctcgc tagacaagaa acaagaattt ggacgttatg
    57241 ttgtgatcca gcgcagaatt atgctttgtc agcaggaact ccaatggtgt cagcaagtaa
    57301 gagaacaaat gcagcagttt tttgagtaag ggataaacag aacctttcta gaaatatcta
    57361 ttgaagagca aattatcttc aataaaagag caatacagtt ggaaaagaaa aagcgatccg
    57421 aacgacaggc caaaacctat cgttcggatc actatttttc tagaactgca ttcaagagac
    57481 tatgacaaaa agcatgccaa ggcctttttt caaataacag tgttccatca ttcattactt
    57541 tgaattgctt ttagcacact tcttgaaagc gggccaacga tgatcaactg caacggtaaa
    57601 gcggcaatca cattcatttt ccaagcattc atataagcag agaataattg atcaggtatt
    57661 ccttgttcca tgatcacacc gaataaagac atacatgtca ccattcctaa aaccatcaag
    57721 catgaaatag ttaagatcat ttggaccttg ctttctggat ttactggtag tttaaaggca
    57781 atttttttcg ccagcacacc gatgatgaag acatccaaaa tgaaagccac cacgaatcca
    57841 ggcactagac caatcaacaa acttgataaa tgaagatctt gatgcagcca taaattatac
    57901 gcgctcatcc ccaagaccat caaaaaacac atcattgttg taaaaattat tccttctttt
    57961 ttatttgtag gcaatctgta ccactccttt aaatttgtca cgaaaatcat cgtagcacat
    58021 ttaaaaattt ctcgtaagta ctttttttat tttctgccct tttatttcat aaacttcatc
    58081 agccgtacgc tcgacaaagt aagaatcatg agaagtaaac aaaatcgttc ccggatatat
    58141 ttgcattagc ttctctaaag cttcgatcgt tgccatatca ataaaattag tcggttcatc
    58201 taacagcaac acattacttg gcttagtaaa aagcaaagca atcgtcagac gggtcgcttc
    58261 tcccccactt aatgtacata acggtttcgt caaagtttcc tgagcaaacc ctaagtgatt
    58321 cagcaaacta cggacaagcg attctgaaaa ctctgtttgt ctcatgacaa aacgaatgat
    58381 ggattcttca gatgtcattt gataaccctt ttgctgatat acttgaaagc ttactttcgg
    58441 agagagaagg attccttctc cttgtttgcg gatatgttct aataaggttg tcttacctga
    58501 accattttca ccgacaatcg cgattttttt acctaaagaa aactgaaaat caccatttac
    58561 cagtaatgtt ctcccacttc tttccaattg gacattttgt cccatgatgg gatagcggct
    58621 gtggagttca agagattttg gtactggaaa acggatttgt ttcatctgct gcggcttggt
    58681 tgtttctcct attttttgga gtcttctctc taatgttttc gcattctttt ggatggcttt
    58741 ttgtacactg tctttttgtt tagaggaaga caaccgatcc agtctgattt gttgcttttt
    58801 ctttttactg gacacttttt ccaacttttc cgcttgtttt ttcttttctt ggatcgattt
    58861 ttccaatcgt tttttctctt tcatgatcga ctctgcttct cgtagctgag tctttttctc
    58921 caattccttt tgagtgagat aggcactata attccccgaa aactctcgga tttctccgtc
    58981 ttttacttcc cagattttcg atgctagctc atctagaaaa tagcgatcat gactaacaaa
    59041 aaccagcgta ccataataat aacgaagctc ttcaatcagt cgatcaatac tttttctatc
    59101 tagatgggtt gttggctcgt ctagaagcat cccttgttca taagaagaaa gaagacgtat
    59161 caactcgatc tttcgttctt cccctccgct ccatttccgc tctgaagttt cttccagtct
    59221 taactggaaa tgactggcta attccaaatt taggtccttt gcttcagcga gataggttaa
    59281 ttgaggcagg tagttgaagg tgatctctgt ttggattttc ccatgatcgg gaaaaagttc
    59341 accggctatt agtttgagca aagtcgattt accgacacca ttttctccta tgatccctat
    59401 acgatcttgc tgatagacac gaagagaatc aatagatagt tctagctggt tgccaaagct
    59461 gacttgtaga tttgttatat ttactgctaa attttccata tgttttacac tcctatttca
    59521 tgtaaaacaa caattatcga tactaaaaaa gagcagaaaa atctgtttgc aaaaaaactg
    59581 catcacatct tttcctactc tgaacgaaat aactatgact aaatcaccaa atctgtcata
    59641 gctttcacct attctactgt cgttcgaaaa aatggacata ctccatccat gaaattgttg
    59701 tttatacatg tcttttgaat ttatttagtt gttattcaaa agttcgaaac gtaatttcat
    59761 cgatgcagtc atttttccga cctccttttt tattaggtta ccacacacga ttcaaaaaaa
    59821 gcaatagcta ctgaatagta actgaaaacc ttttattcga acatagtgat cttttctggt
    59881 gctaaacgaa cagactcgta accttcttct accgcttttc ctattgataa aataataact
    59941 ggaacataac ggtctttatc caaaccaaat gcctctgcca actgatctgc ttcgaatcca
    60001 ccaattggat ttgtttcata gccatgatca cgcgcaacta acatgaattg catagccgct
    60061 aagctagaat cgactttgac tacatcgttc atttcttctc ttgaaaaact cttgtaataa
    60121 ggaatgatcg cagctaattg ttgatctctg acttcttttg gcatcttccc tgattcgtaa
    60181 gcttggttgt agatttcttc gccatattcg taacattgca tatcgccaaa aatcatgacc
    60241 attgcagaag aagtatcatt ttgtctcgta ttgaaacgga tcaaaggacg caagatttct
    60301 tttgcttcct cgctttcagc gaccacaaag cgccatggtt tcatattgac agaagaaggt
    60361 gcagtcgtcg cttctttgat catttccagc atttcttcgt gtgggatttt gtatgtttca
    60421 tcatatacgc gaacggatct tctaccagtc ataattgttg aaaaatcatt tttttgttcc
    60481 attattatta acctctttcg tttctgttgt tatattgcaa agttgggtat tcaatttatc
    60541 taaaagtgtt agaaattgtt tcatttctac atcgctgaaa gccggggata gcacggaacc
    60601 tgtatcttta tggtccattt cacaatgctt gattttcttc tgggcttttt ctgttagcgc
    60661 tacatacact tcccggttgt tctgaggatt tctctgacgc gaaacatagc catttgcttc
    60721 caataccttc agatgacgag taactgctcc ttgatcaatc tgcaactttt cttgcaaact
    60781 gctttgtagc aatggatttt cttctttcag tctcatcatg atctcgtatc tagtaagact
    60841 tataccgata ttttcttcaa agatctggac gattttttga tcggctaatt tgatttgata
    60901 taaaaggtga ctaatctctt ctaaagacat aatttcctcc tttctaataa ttgactcgtc
    60961 aacgattgac ttgtcaataa tagaataaaa aaagaaaaaa gtcaaagaat cagctgtgta
    61021 ataaaaaaaa cgctctcaat agaaaagtat agagaaaaga aattttttgt agaatgaacg
    61081 gaaaaaatga taaagaaagc acaacgctaa aaccaagatt attgattctt atcaaactat
    61141 agaagatagc tcttctgcag tagataattt tacaccgcca aaaatttccg aaatcgcctt
    61201 ttgcaagaat cccgtcgaat attagaaatt ggatcagtat gcttaaacag taataaaaaa
    61261 gttatgacaa gatgttgtca caacct
//
LOCUS       DAZKNH010000005        55732 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000005 DAZKNH010000000
VERSION     DAZKNH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.005"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(363..3074)
                     /locus_tag="ACG8MG_000297"
     CDS             complement(363..3074)
                     /locus_tag="ACG8MG_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFQ0374376.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(3084..3785)
                     /locus_tag="ACG8MG_000298"
     CDS             complement(3084..3785)
                     /locus_tag="ACG8MG_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0374377.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            3971..4570
                     /locus_tag="ACG8MG_000299"
     CDS             3971..4570
                     /locus_tag="ACG8MG_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0374378.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            4651..5664
                     /locus_tag="ACG8MG_000300"
     CDS             4651..5664
                     /locus_tag="ACG8MG_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFQ0374379.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            5772..6689
                     /gene="rbsK"
                     /locus_tag="ACG8MG_000301"
     CDS             5772..6689
                     /gene="rbsK"
                     /locus_tag="ACG8MG_000301"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFQ0374380.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            6717..7112
                     /gene="rbsD"
                     /locus_tag="ACG8MG_000302"
     CDS             6717..7112
                     /gene="rbsD"
                     /locus_tag="ACG8MG_000302"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFQ0374381.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            7112..8485
                     /locus_tag="ACG8MG_000303"
     CDS             7112..8485
                     /locus_tag="ACG8MG_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0374382.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            8842..10986
                     /locus_tag="ACG8MG_000304"
     CDS             8842..10986
                     /locus_tag="ACG8MG_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0374383.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(11038..12078)
                     /locus_tag="ACG8MG_000305"
     CDS             complement(11038..12078)
                     /locus_tag="ACG8MG_000305"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0374384.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(12355..14157)
                     /locus_tag="ACG8MG_000306"
     CDS             complement(12355..14157)
                     /locus_tag="ACG8MG_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0374385.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(14171..14923)
                     /locus_tag="ACG8MG_000307"
     CDS             complement(14171..14923)
                     /locus_tag="ACG8MG_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0374386.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(14932..15294)
                     /locus_tag="ACG8MG_000308"
     CDS             complement(14932..15294)
                     /locus_tag="ACG8MG_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0374387.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(15470..16342)
                     /locus_tag="ACG8MG_000309"
     CDS             complement(15470..16342)
                     /locus_tag="ACG8MG_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0374388.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(16393..16881)
                     /locus_tag="ACG8MG_000310"
     CDS             complement(16393..16881)
                     /locus_tag="ACG8MG_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFQ0374389.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(17019..17810)
                     /locus_tag="ACG8MG_000311"
     CDS             complement(17019..17810)
                     /locus_tag="ACG8MG_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFQ0374390.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(17865..18473)
                     /locus_tag="ACG8MG_000312"
     CDS             complement(17865..18473)
                     /locus_tag="ACG8MG_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFQ0374391.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(18466..18783)
                     /locus_tag="ACG8MG_000313"
     CDS             complement(18466..18783)
                     /locus_tag="ACG8MG_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0374392.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(18939..19667)
                     /locus_tag="ACG8MG_000314"
     CDS             complement(18939..19667)
                     /locus_tag="ACG8MG_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFQ0374393.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            19826..20707
                     /locus_tag="ACG8MG_000315"
     CDS             19826..20707
                     /locus_tag="ACG8MG_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0374394.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(20944..21987)
                     /locus_tag="ACG8MG_000316"
     CDS             complement(20944..21987)
                     /locus_tag="ACG8MG_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0374395.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(21990..22658)
                     /locus_tag="ACG8MG_000317"
     CDS             complement(21990..22658)
                     /locus_tag="ACG8MG_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0374396.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            22800..25136
                     /locus_tag="ACG8MG_000318"
     CDS             22800..25136
                     /locus_tag="ACG8MG_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFQ0374397.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(25251..25562)
                     /locus_tag="ACG8MG_000319"
     CDS             complement(25251..25562)
                     /locus_tag="ACG8MG_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374398.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            25743..27218
                     /locus_tag="ACG8MG_000320"
     CDS             25743..27218
                     /locus_tag="ACG8MG_000320"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFQ0374399.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            27265..27849
                     /locus_tag="ACG8MG_000321"
     CDS             27265..27849
                     /locus_tag="ACG8MG_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFQ0374400.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            27851..28270
                     /locus_tag="ACG8MG_000322"
     CDS             27851..28270
                     /locus_tag="ACG8MG_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFQ0374401.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(28551..28835)
                     /locus_tag="ACG8MG_000323"
     CDS             complement(28551..28835)
                     /locus_tag="ACG8MG_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFQ0374402.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(29187..29897)
                     /locus_tag="ACG8MG_000324"
     CDS             complement(29187..29897)
                     /locus_tag="ACG8MG_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFQ0374403.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(29894..31366)
                     /locus_tag="ACG8MG_000325"
     CDS             complement(29894..31366)
                     /locus_tag="ACG8MG_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0374404.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            31504..31977
                     /locus_tag="ACG8MG_000326"
     CDS             31504..31977
                     /locus_tag="ACG8MG_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0374405.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(32130..32681)
                     /locus_tag="ACG8MG_000327"
     CDS             complement(32130..32681)
                     /locus_tag="ACG8MG_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0374406.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            32786..33316
                     /locus_tag="ACG8MG_000328"
     CDS             32786..33316
                     /locus_tag="ACG8MG_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFQ0374407.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(33799..34242)
                     /locus_tag="ACG8MG_000329"
     CDS             complement(33799..34242)
                     /locus_tag="ACG8MG_000329"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0374408.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(34258..35418)
                     /locus_tag="ACG8MG_000330"
     CDS             complement(34258..35418)
                     /locus_tag="ACG8MG_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0374409.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            35925..37625
                     /locus_tag="ACG8MG_000331"
     CDS             35925..37625
                     /locus_tag="ACG8MG_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374410.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(37727..38560)
                     /locus_tag="ACG8MG_000332"
     CDS             complement(37727..38560)
                     /locus_tag="ACG8MG_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFQ0374411.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(38864..41029)
                     /locus_tag="ACG8MG_000333"
     CDS             complement(38864..41029)
                     /locus_tag="ACG8MG_000333"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFQ0374412.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            41289..43583
                     /locus_tag="ACG8MG_000334"
     CDS             41289..43583
                     /locus_tag="ACG8MG_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFQ0374413.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            43576..44241
                     /gene="pgmB"
                     /locus_tag="ACG8MG_000335"
     CDS             43576..44241
                     /gene="pgmB"
                     /locus_tag="ACG8MG_000335"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0374414.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            44234..45241
                     /locus_tag="ACG8MG_000336"
     CDS             44234..45241
                     /locus_tag="ACG8MG_000336"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0374415.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            45263..46282
                     /locus_tag="ACG8MG_000337"
     CDS             45263..46282
                     /locus_tag="ACG8MG_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0374416.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(46563..47930)
                     /gene="nhaC"
                     /locus_tag="ACG8MG_000338"
     CDS             complement(46563..47930)
                     /gene="nhaC"
                     /locus_tag="ACG8MG_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0374417.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(48039..49460)
                     /gene="tyrP"
                     /locus_tag="ACG8MG_000339"
     CDS             complement(48039..49460)
                     /gene="tyrP"
                     /locus_tag="ACG8MG_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFQ0374418.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(49703..51580)
                     /gene="tdc"
                     /locus_tag="ACG8MG_000340"
     CDS             complement(49703..51580)
                     /gene="tdc"
                     /locus_tag="ACG8MG_000340"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0374419.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(51871..53127)
                     /gene="tyrS"
                     /locus_tag="ACG8MG_000341"
     CDS             complement(51871..53127)
                     /gene="tyrS"
                     /locus_tag="ACG8MG_000341"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFQ0374420.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(53239..53444)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            53707..55008
                     /locus_tag="ACG8MG_000342"
     CDS             53707..55008
                     /locus_tag="ACG8MG_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0374421.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(55077..55676)
                     /locus_tag="ACG8MG_000343"
     CDS             complement(55077..55676)
                     /locus_tag="ACG8MG_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0374422.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        17022 a        11424 c        10005 g        17281 t
ORIGIN      
        1 tttgtaaaat ttatctttat acattctaag aggtctgttg cccaataaaa aacaagagat
       61 cagacatgta tttgtctatc ctcttgttta aaatagcaaa atattcttgt ttcttctata
      121 aatggtgttc aaaagctttt atctacggca atcagtcttc tcaaatgtaa aacatgagga
      181 gctaatttta tttgagtatt ctttttgctc gatgcaggac ggctttttta caacctctga
      241 ttcatggtgt tcaatcagct gtccttcggt attagctcaa aaaacggcaa aatatgaaaa
      301 actattttgc cgtttgtttc tctaatactc aggtcaaacc gctgatttca caacctctgt
      361 ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt gaaatgggta
      421 acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac ataagcccac
      481 catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa gatgaagtca
      541 gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa gatgatattt
      601 tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt tattgtagat
      661 aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac gataaaagcc
      721 agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc cattgattct
      781 tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc tgtggcaagc
      841 agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg cagcaaaaat
      901 gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga catataaata
      961 aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc tttttgatct
     1021 tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc tactttttgc
     1081 gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc agttaaatgg
     1141 ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact ttgcgaagca
     1201 tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc atttactttt
     1261 tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga tgaatccaat
     1321 gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac agaacttaga
     1381 gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat tccgataatc
     1441 gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt ttgattaaag
     1501 ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc tggcttcggt
     1561 gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa aacgatcatt
     1621 gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg cggccagtca
     1681 taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa aatgatcctt
     1741 ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga ggctagactt
     1801 gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc ctttaatgtc
     1861 ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt gatcaatgcg
     1921 gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg atcagacaga
     1981 tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta tgatggttta
     2041 gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga ggccagttca
     2101 cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg ttgagataat
     2161 gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc tgactgttgc
     2221 gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg tgctttgcca
     2281 tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt cagctcttct
     2341 aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt ttgctccatt
     2401 ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa atcaagatat
     2461 aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa ataatcgacg
     2521 ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa tggagaacgg
     2581 acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat ctgataaata
     2641 tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg gtttggtttt
     2701 tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt gttataagaa
     2761 aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt tcctgtttct
     2821 acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa gccaccagta
     2881 gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat cgttttttgc
     2941 atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat gacaataagg
     3001 atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc tttaagatat
     3061 gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga aggatgatca
     3121 tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc agcgatgggc
     3181 gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct gctagcgttt
     3241 tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt cggttcatca
     3301 cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct ctgctgttcg
     3361 cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac ttgcctcaat
     3421 acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac attttctttt
     3481 gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc tgttcgtcgg
     3541 taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat aatgatctga
     3601 ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt tcctgctcca
     3661 cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat atcgtcattt
     3721 gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac ttcgacatac
     3781 ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat cgaatgcgtt
     3841 ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg aatcctttaa
     3901 tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt tatttgaaag
     3961 aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa aaactgcttt
     4021 tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc cacacactgc
     4081 agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta ccgctgcctt
     4141 gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac aaactgatat
     4201 gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga aggattcttt
     4261 gaaagataca gataatatgt tgactatcac aaaatacaat ccaagtgctt tttttggtac
     4321 ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa gcggtgtagc
     4381 gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt accatatcgt
     4441 gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt tcataaaaaa
     4501 gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag ctatccaaca
     4561 aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt catgggaaaa
     4621 taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac aatcaaagaa
     4681 gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa tggaaatacg
     4741 gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga attaggctat
     4801 cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat tggtgtactc
     4861 gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga ggatatcttg
     4921 tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca aaaagaaatc
     4981 gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc gacttctgct
     5041 gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt cattgttttg
     5101 gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt tcgcggtggt
     5161 tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct tgtctatcct
     5221 gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc tcttgatgtt
     5281 taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa acaaggtggc
     5341 taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc tttaaatgat
     5401 gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat ccctgaagac
     5461 tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc gaaactgaca
     5521 acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt gttagatcgg
     5581 atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt agaaaaaaga
     5641 ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc gaactattaa
     5701 aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg agatagttgg
     5761 aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac actacccttc
     5821 gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt tttcacgctg
     5881 gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca cggacatctt
     5941 ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta ctgacaaaag
     6001 aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga caagcaatga
     6061 ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca aataatgctt
     6121 tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac tttattattg
     6181 ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca aaagacgcag
     6241 gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact gaactacttg
     6301 aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca ggaatcagag
     6361 tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta ggtattggaa
     6421 cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa gaacacggta
     6481 tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat acatttattg
     6541 gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg atcctttatg
     6601 gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct atcccttacc
     6661 gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa acaaacatga
     6721 aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg gggcactttg
     6781 ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa aaaatagatt
     6841 tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt ttagaagaac
     6901 ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg gaaatattgg
     6961 aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat gaagaaatga
     7021 aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca ccttacgcca
     7081 atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc ctgaacagat
     7141 ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag acaatattga
     7201 ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc cagtagccaa
     7261 tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac tcgatagata
     7321 tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga cgcgtcaaat
     7381 cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg gtttgatatc
     7441 ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa aaggtctggt
     7501 tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc atattttgtc
     7561 tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt ctgctgccaa
     7621 aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga ttgaaccgaa
     7681 tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg ggattcctgt
     7741 agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt tcttttattc
     7801 ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag tatttgtacc
     7861 tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc ctgcgggtgt
     7921 ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta acggaccgat
     7981 cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta tccaatgggc
     8041 tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa tcgttccgtt
     8101 gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc tgcgaagtaa
     8161 acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac ttgcgggagt
     8221 gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg ttgctgcttg
     8281 cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag cgatcgcttt
     8341 tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt tctggtactt
     8401 agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta gcgggtttga
     8461 tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag atgtttatca
     8521 catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt gatactttcc
     8581 aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt tatccccaaa
     8641 caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg ctcgttgctt
     8701 atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa aagcttttcg
     8761 acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga aaatcaatga
     8821 aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct agctgctact
     8881 tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga taatcaaata
     8941 gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag tgaaacaacg
     9001 gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc gaccgagcca
     9061 actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac atattccctt
     9121 acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc ggattcccct
     9181 gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt agcggaaacg
     9241 aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt cgctgttaat
     9301 ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa caatttatct
     9361 attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac tgatcctagt
     9421 gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt gggtaataac
     9481 aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa cagcgggaat
     9541 tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca gagagaacca
     9601 aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc tcgtccaaat
     9661 acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt tgttgtaaaa
     9721 tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc aatcgttcga
     9781 gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc aaatactttt
     9841 gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta tatcggtggc
     9901 tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc ccaacctggc
     9961 gacctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat ctatattggg
    10021 aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac ttctatttca
    10081 gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc tgcttcaggt
    10141 tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca tttcagtcaa
    10201 aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga aatcgggcaa
    10261 aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag tggatctgga
    10321 aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg atcatataaa
    10381 ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag ctaccaaatc
    10441 cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta tgccgacttg
    10501 atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa gtcggaaaca
    10561 aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga tcgccaatat
    10621 tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga tgaaccgaaa
    10681 aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc taagcgattc
    10741 gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga attttcaacg
    10801 ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg ggatttgtgg
    10861 aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa agctgcaact
    10921 gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct gcctttaaag
    10981 aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta atttattcta
    11041 tttctttata tttattttgt attccgttac aaactcgcgt acttggttag gttccagagt
    11101 gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct caaatgtgat
    11161 accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc caaaatttgc
    11221 tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt tttcatctgg
    11281 gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa atggatgatc
    11341 gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac ctaatttttt
    11401 aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg taggcgttgt
    11461 atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt cgtcgattga
    11521 ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga atactgtttt
    11581 gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca gcgtataccg
    11641 tacttctaca tctaaagttc caggaaatcc gttttccata tctgggctag tctgatagaa
    11701 aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct tggcttcaaa
    11761 gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta cttcgaacgt
    11821 ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc cgattgaagc
    11881 accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta agatcaactc
    11941 tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa gatcactgac
    12001 agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt cgccaaaatt
    12061 ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac tgattccagt
    12121 atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa aatttactat
    12181 attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca acctcttttt
    12241 ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat
    12301 atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc tttttcacaa
    12361 tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt agatgaaata
    12421 caatacgaag aatatcgtaa acggaaccca aatatctttg tatggatttt gcatgaatag
    12481 accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg gtgctaagaa
    12541 taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg ttcctatctt
    12601 ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct tgaacatcaa
    12661 acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa attcaaaacc
    12721 agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt actctacttt
    12781 ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg gtttcaggtt
    12841 tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt tcagttgatc
    12901 aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca attcatagct
    12961 tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt tttctttcgt
    13021 gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag ccgcaaattc
    13081 acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag cagaaagtgt
    13141 tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca tcactagatc
    13201 atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta atcctaccgt
    13261 caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt gtgtatattc
    13321 acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca atgaaatctg
    13381 ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa tgaaataagt
    13441 tcctaatacg atcgtaacta aggctacccc tataccaaag acttgatagg tccccacgtg
    13501 tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt caaggaaaaa
    13561 caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca atgctaaaat
    13621 cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca aaacagcaaa
    13681 aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg aaatattgag
    13741 atctaactga tccactaata atcggtggac gatacttgtc aatcctacac cgacagctga
    13801 acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca tctgtgcgat
    13861 ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc gcatcgtcat
    13921 caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta aaacagaacc
    13981 gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa aagcttcgtt
    14041 gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc ctgagaataa
    14101 caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc aaagcatagt
    14161 caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga tagaaggaat
    14221 cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg atgaataaaa
    14281 tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc gatacatgat
    14341 cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg gaatccagcg
    14401 cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca cgtgcggcgg
    14461 cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa atatgagaga
    14521 tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt ctgacgcctt
    14581 gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat aaattgaaat
    14641 cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt ccttttaatg
    14701 tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct aatgtggata
    14761 aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca ataaattcgc
    14821 ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca ttgttttttc
    14881 cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc cctacccttt
    14941 aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt catacgaagt
    15001 gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa gcggtcgagt
    15061 caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc caggcaggat
    15121 atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt ttgtaccatc
    15181 tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg cgattttcac
    15241 ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt tcactttttc
    15301 cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct gccttcgaat
    15361 caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa aaagagttca
    15421 agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt cacttacggt
    15481 ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg attgagacaa
    15541 gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct tgtccatctt
    15601 cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg gtttgtcgag
    15661 cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt tctgttgcat
    15721 aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct aaacgctctt
    15781 gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc gtcaaatcat
    15841 tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct gtctcacttt
    15901 ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct gtatcaacga
    15961 ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca ttgactttta
    16021 aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt gccaaaggta
    16081 tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa aacaaaatta
    16141 cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac gtgatcaacc
    16201 aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct aaatagaaga
    16261 aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa ccattgatat
    16321 aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca ataaatatct
    16381 aatgttactt ttttaatttt tttttcgaat acgcgccatt acaccttcta gttctaactc
    16441 tttgtcttca gtgatgtacc aaccgcttgc ctgtgtccct ccggctgcct gcggtaaata
    16501 atccaatttt gttcgaaccc agtgcttttc ttctgtaaaa tagtcttcta ccaagacctc
    16561 tagatccatc cctgctgaaa gaggcaaatc ttcccgaaca gatttaaaga taaatcgttc
    16621 cttcttccca tcatacttca atattcctgt atcgataatc ggtaaagaat aataatccag
    16681 ctctttcttc accgtcaata aatgtttgaa aacttcatgg tacacttgct ctaattggcg
    16741 accttctgaa ttgttcgggt aattaaccat cactttttct ttgtctggat catagaaggt
    16801 ttggcttagt tgagtgatat aagtatgtaa tctttccatt tctgttttta acttttctct
    16861 ttgaccttct tctaccttca tatactcttt caccctgttt tctttttaga tattttcttt
    16921 cttattttac tatagttatg tgcgttaagc gatgaaaaac agcaaagatt ggaaggatct
    16981 ttgctgtttt ttcttaaaat atccagttga gtacaggatc aatctgtttc attgtcctct
    17041 tgatccgtcg taatattgat cgatgtgtct ttagcagtga tctttagctt tttctgtcct
    17101 tgttctaaca aagtatttgt tattggctgg tagtcttttt caatataaga ttcaccagac
    17161 gtttggatag tatagccact gttttccatc tcatgcaaag ataaattgcc accgtttagg
    17221 atgacttttc cttctttgct gatcaaactg tcttctagat ggaagttgct gttttcacct
    17281 gtgacgatca agttatccac attggcctca tctaatcgaa gtgagcctaa ttcatctttc
    17341 gcagttattg ttcctcctgt aaattcattg aacgataccc agccatcatg tgtcttagta
    17401 ttcacttttt tgattgatac ttcgttgata tcaattgcac tattattccc ttcgattgtc
    17461 aattcagata attcatgatc tgcagggaca acaatagtga tttgtgcact tgtgtcttct
    17521 aaactaatga ttgcacctgt tgattttttg actgcattca cagtcaacac gccatttttc
    17581 tcctttattt ctggcttttc tgtcttatca taagtcgttt ttatctcaaa atccaatccg
    17641 cgtatgaccg aaactgtttg attttctgca tggatgacga ttttgtttgc atccttgatt
    17701 ttttttgtct cagtaaccat atcgatcaac cgaggaccat tatcccaagt cagactcttt
    17761 tgagcgccta atgtataagt tgcacctgct aggaggccac cagtaagcat cagaccaata
    17821 ccggtaatca aaaaatgttt caatctcaat ttatgtgtcc tccttcaaac gcccgctgaa
    17881 tgcagatctc tttttgtaat aaaacgtcgt cccatccatt taacaaagtt catcatgaca
    17941 taaaaaagcc cttttgttcc tttcagcaac agtggcgaaa taaatactgc gcttcctgtg
    18001 gcgaacagac cgacaccgat aaagaataat ccactcagaa tcgattgtcc aaacacactg
    18061 attcctgaga tgatcatgaa taggccaaca cctaagatac tgatacataa aacatagata
    18121 ctaaaaatga gcgctagtaa agcaaccaat agtcccccaa taaccaatat aaaccccagt
    18181 gctagtggaa tgaggacagg agaagcaaat aaaccgataa tgattaacca aatcaggcga
    18241 atttgtcttt ttggattttt cactaactcg ttttctgctt gttcttcttc tgaaaagtaa
    18301 ctgattttta attttaaggc aaactgcttt ggtgtaccat attcatcaat gatctgatct
    18361 aatgtttttc cagaatctaa aaaatattcc tcataatact ggatcaaatc aaaacgatcg
    18421 tcttcaggaa tttcttgaag atgatccaca acttcattca aatattcatt catttgtgct
    18481 ctcctccaat aaccggtcta aagaatctcg aaaaacaatc cattcatcaa taatttcttc
    18541 taaatgacga cttcccattg gtgtcaacga gtaatatcga cggtttctgc cttcaaaagg
    18601 ttcatcatag gtctcaagat atccttcttt tttcaatctt ctcaaaaccg gatatagtgt
    18661 agattcactg acagagagac tttcttgtac gatttttgtt aaggcatacc catacatatc
    18721 ttccttgctt aatgctgcta gcacagtacc atcaagaaga acagttggaa tctggatgtg
    18781 cattacgtca ccttctattc tcaagtgtaa tattttattt caatctatat tatacaacat
    18841 ataatatagt ttcaaaatca gtttgtcaaa tacaaaaaaa ctgactaaaa gatttgatcc
    18901 tttttagtca gcaaaaaatg gcttctcttt gatttttatt ataacagtgg cataatccaa
    18961 tttgtccaca tccagctcag tggcatgata acgatcattg caggtatcat gtcagctact
    19021 gggaacattt tcacgttgat cattctaaaa ccggtagcga ccatcaagaa tccgccgcaa
    19081 gctttaaaat ctaagatcat gtctggtgta gtcaatggat aaataaattt tgccaataag
    19141 aacaaaatat aaaaaataat gaattgggga attgctatca cagaaacgac ataccctaaa
    19201 ttacacgcaa aaatagctgc tgtgaaaaaa tcaaggacag atttagaaat caaaatcgta
    19261 ctgtctcctg tcatcccaga atctaaactt ccatagatcc ccgtaccact tgcacaaaat
    19321 agaacaatga ccgtcactaa tgtgctgata aattcatcgt gggtcatacc aagcttttca
    19381 ctagggaaga ttttagaaat aggtacttgc atcagcgcag cccccttatt tatcccatta
    19441 cctaaatgaa caatcaaacc aatcgctgtt cctatcacta aagcaaaaat aacagccggc
    19501 atatttttca tcggcgcaat ggaatagatt cccatcccca tcgaacacac accaaaaacc
    19561 atcgtcagct gtgctttaaa atgttcactt aatttatttc ctactaatcc gccaattagt
    19621 ccgccaaaca aaacggaacc tacattgatc aaaactccag ttggcatcaa aaaactcctc
    19681 tctttttcta cgaagcgaaa aaaatcagaa aaatttctgt aattattgct ttcgctgcaa
    19741 gaaagattgt aacagacaga gaagattaca ccaattcatt ttttgtgtta aagtattctt
    19801 tattggaata agtaggagga atatgatgga tatccataaa ttcgaagttt ttcttgattt
    19861 agccgaaaca atgaatttca ccaaaactgc tgatcgacaa ttcaccaccc aaggaaatat
    19921 ttctaaacag attctttcgt tagaaacaga attagacgtc aaattattcg aacgcgccca
    19981 tagaaaaatc gaattgaccg aagctggaag cttattgctc ccatatgtaa aaaatgtcgt
    20041 agaacaatac catgctatgc aagaagtgct tgaaacctat acgaaagaca acaatctttc
    20101 attaaatatc ttaacgatac cgacaatgat caattataaa ggtttttcca aaatcaccga
    20161 gtttttgaaa cgccatcctg aatttacagt ccagttgaaa gaagtggaaa gtgtcgaatt
    20221 aacttacaat gacgccaatg aacatagtga cacgatccac tttgctagaa gtttccaggc
    20281 cccaccaagt tcgattgagt ggttgcctac tgaaacagac gattttgtag cagttctacc
    20341 taaaaatcat cctttagcta gtaaaaagaa attggatcta agcgaactga aaaaggagaa
    20401 ctttttgctc cttggcccca ctacgaactt gtatcaacct gtactagatc tgtgtcgcga
    20461 agctggattt gaaccaaaaa tctcttataa aggagcaaga atcgacttga tcatcaatat
    20521 gattgcaaat gacatgggga ttgctattgt tatggaaaaa acagtcaaga atctattaaa
    20581 agaaaatact gtgattctcc ctatttctcc aacaacagag agttatcttg catttactcg
    20641 taaagtcggt gagcattctt tggcttcaga tacattttgg gagtatttaa atcaagaaga
    20701 aaactaagcc ctacattttc tgtccatcta tccacccaaa aaaagagagc gacgcatgac
    20761 tttcgctctc tttcttgcat catgtccttg tttcaatgac acctctaaca tttctcgtag
    20821 ttcaataaga aagcagcgat ttttatgcca aaagaaccgt aagaacattt ccctaatcac
    20881 aaaaaaacga ctaaaaaccc ctaaaaacct tcaatagaat ttttaggggt tttattcttt
    20941 ttcctattgt ttacttaacc gtatcattgc tgtcgttcct actccttcga tactgctata
    21001 atcaatacgt ccatcatgca gttctagaat ttttttaacc agtgaaagtc ctagcccatt
    21061 cccagaaatt tgacgacttg tatctccttg ataaaatttt tcaaacattc gatctctggt
    21121 ctcttcattc attcctacac ctgaatcggt cacttccagt acaatctctg tggctgtctc
    21181 aaatagtttg atatgaatct gtccattgat ttgattgtac tttatggcgt tatccatgat
    21241 attcagccac acttggtaca aaaactcttc attgcctgtg taatttactg cagcaagttc
    21301 gatatccaat tctagctgtt ctttctccca tttaggctga aaaaaaacga ttacttctct
    21361 gatttgttca tctaatcgat actctttctt ttccagttgg atacgctggt tttccagttt
    21421 gttcaatttt aagatgttct ctgttaattg tgaaagctgc gtgatacttt cgataatccg
    21481 atataaaaat atttggcgtt cttcgtcaga gagatttggg gcttgtaaca attgaacata
    21541 tccttggatt gttgctagtg gtgttttgaa ttcatgggat acactggaca caaagtcatt
    21601 cctcagtgtc tcgatactat tgagttcttg gaccataacg ttgaaatctt tatacagttg
    21661 ctgtacttct gctactttct gctgttcatc cattcgaatc gaaaaatctc ccgttgctac
    21721 cagactcata tttgtcccta gcgcactgat tggatgcagg atacgccgcc caacaaaaat
    21781 cgatattcct gtacccacga ccatactgaa ccccgcaaag acgaataacg ggtataacgt
    21841 tggttttcca gccgattcag caggcgagat agaatgtcga gacaataaga aaataaaacc
    21901 gagaaaacac agcagtgtca ctaagatcga aatgaagaca atactagcaa aatagatcca
    21961 taattgtgaa aatagctttt ttttcatctt taaataaccg ccttatagcc tagtccccgt
    22021 actgtgatga tttgaaaatc ttccgtcttt tcaaaacggg tccttaaacg tttgatatgt
    22081 acatcgatcg ttcgttcttc tgtattcgta tctaatcccc aaatatcatc catcaactgt
    22141 tggcgagtga agatcttgtt gggataagac agtaatttat acagaagcag aaattctttt
    22201 tgaggcagaa tcaacgaatg ctgatcttgt aacactgtat aagcatctag ctctaatttt
    22261 gtttgtccga ttatcaagct actgctgtaa ttgatctttg aacgtcgaag caatgcttcc
    22321 acacgtagga tcatctcatt gacatcgatg ggttttgtca tataatcatc tgctcctaat
    22381 ttgaacccgc gtttcttatc ctcaaaatcg gcctttgccg taatcattag aacaggtgtg
    22441 tcaaatctgg tgctacgtaa tgagtctagc aagtcatagc catccatttt cggcatcatg
    22501 atatctgtaa tgattaattc tatacgtgtc gcttctagtt ttttcagcgc atcctctcca
    22561 ttcaatgcgg tgatgactga aaagcccact cgttccaaca cagaacggta aagcaactgt
    22621 aattgcggat catcttctac tactaaaata cgattcattg atctccctct gttttttgaa
    22681 aaatttctag attttttctt gtagttcatc ttcagttaac atttatttcg tatactacga
    22741 tcattgttct gatatactta ttagtatacc gaatatttca atacgggaga agggtgatta
    22801 tgcttcaatt aaaagatata aaaaaatatt ataaagtcgg cgaaaccacc accaaagcat
    22861 tggatggtgt gtctgtcgct ttcagacaaa aagaattcgt agcgatttta ggtcctagtg
    22921 gttctggtaa aacgactatg ctgaatgtga ttggtggact agataattat gattccggtg
    22981 atatggtaat caacggaaag tctactaaag actttaagga cagtgactgg gatgcctacc
    23041 gaaacaactc tatcggtttc atttttcaaa gttacaactt gattggtcat ctaggcatta
    23101 ttgaaaacgt tgaacttggg atgacattaa gtggtgtttc taaagacgaa aaacgaaaac
    23161 gggcagaaga tgcattgcac cgagtcggct tgacaaatca tatgcataaa aagccaaatc
    23221 agttatctgg tggacaaatg cagcgagtgg ccatcgctcg agcactagcc aatgacccag
    23281 atatcctttt gtgcgatgaa ccaaccggag cgttagatac agaaacaagt atccagatca
    23341 tggaattgat ccaagagtta tcgaaagaaa aattagtgat catggtcaca cataatcctg
    23401 aactggccca tcaatacgca gatcgaatca ttgaattttc tgatggtaag atcttaacag
    23461 attcccatcc tcacatcgaa cgaccaaaag atgatcaatt taacttacgc agaacaaaga
    23521 tgagtttttg gacagcgtta aagctttcct tcaacaatat ccgaacaaaa aaaggacgta
    23581 ctttcttgac ctcctttgct tccagtattg ggatcatcgg aatcgcgatc gttctgtccc
    23641 tttcttctgg attccaaaaa caaatcgata acacgcaagc tgaaacgatg gcgaaatttc
    23701 cgattactat ttctaaggta acgactaacc agactcgtga cgatgccgga ttgggtgcaa
    23761 gcaaagcgga ttatccagac agcaaaacga ttactgcaaa agtcagcgaa gaagataaag
    23821 cacagcacac gaataagatt gatcaaacat acgtcgatta cgtaacagac atcgatccta
    23881 acttaagtaa taatatcgga tttacccgta ctacaggaat caatctttta cgtgatgtaa
    23941 atggcaaagt acagcctgtc agcttctcga atcaaaatcc tgatacggaa tctctttctt
    24001 tatctagtac gatgtcagca atgacaggtg tgggagtgtc aagtttccct acacagcttg
    24061 acactagcaa agaaaacttt ttaaaagaca attattcttt acttgccgga agttatccag
    24121 cctcagcaac agatgtcgtg ctgatcgtag acggcaataa taatactaat atcaacgcat
    24181 tgaagaatct tggatttgat gtgaaagaag acgaaaaact tgattttgat gatatcgttg
    24241 gtacaacatt caaattagta aacaacaata cttactatac aaaacttcca actgggaatt
    24301 ttatccctaa tacagattat gatgcgatgt atcagaatgc ttctgatgaa ttgaagatct
    24361 cggggatcct acgcgtgaaa tcttcttcta caatgaatct cttatctcca ggaatcgctt
    24421 atagcgatca attaacgact caaatcgtca acgaaaacaa agaatcagaa atcgtcaaag
    24481 ctcaaagaga cagtggtgta aatgtcttaa ctactgaaaa agttgacgaa aatgccaaac
    24541 aaacattgct tagttattta ggtggagaca gcttgccttc aagtatcatg atttatccga
    24601 acaattttga agacaaagaa aagatcttgg attatctaga tgattacaat aaaggcaaat
    24661 cagatgaaga taaaatcatt tatactgatt tagctggaac gatgactgaa ctgactggcg
    24721 gattgatgga tgctatcact tatgtcctta ttgcttttgc agggatctct ttagtaacaa
    24781 gtatgatcat gatcagtatc attacttata catctgtgat tgaacggaca aaagaaatcg
    24841 gtgtactaaa agctttaggt gcacgtaaaa aagacatcac tcgcgtattt gacgcagaaa
    24901 cgtgtatttt aggtatttct tctgggatat taggtgtgtt tattgcatgg ctagctacgt
    24961 tccctatcaa tagcatctta tacaacatga cggatctaaa aaatgttgct caactaaatc
    25021 ctgtccatgc gattattcta gtgatcgtct ctactatatt gacgatgctt ggcggtcatc
    25081 ttcctgcacg aatggcagcg aaaaaagacg ccgctatcgc tttgcgtgca gaataaggtt
    25141 gtgacaacca catttagatt tgagtcataa gagcgctgaa atagtagaaa acagcaagaa
    25201 acgaggaagt aatatgttcc ttgtttcgcg ctgttttttt taattatttt ttataattct
    25261 ggtatacttt tctttccttt gtaccacaac tcttctggcg tatacctgtc ttttccattt
    25321 atgaatactg ttccatctgc ttcaatcgtt acttcagtcc caatccacat attacgagaa
    25381 agtgcgagaa gattttttct ttttcctctt cctttttttc caaattgcaa ggcatcggtt
    25441 atctcttctg tccaatgaga tttcacgtat tttacttctc cgttttcaaa tgtaactttc
    25501 aaaatattcg ctatattttc cacttttact gctttaggaa atgactcctc catttttttc
    25561 actttccttc ctccaacact aagtatgcgc tttcttttag tgataagtat atcatagttt
    25621 attctattat caaaaaaacg catccattcc attgtagaat actttaccga acaagttgaa
    25681 ttgttttcct ctttttcacc ttttactata gataggtaaa gacaactaag gaggaatctc
    25741 tcatgacaga tcaaccgtat gatttaagaa aagtcatcga agaactaaaa acacttccag
    25801 gtcaatatca cgaaacagat attgaaatcg atcctgaagc agaattatct ggtgtttacc
    25861 gttatatcgg tgctggcggt acagtaaaac gtccgactca agaaggacca gctatgactt
    25921 tcaacaatat caaaggtttc cccggcacac gtgtgaacat cggaacaatg gctagtcgta
    25981 aacgtgtagg tgaaattttg caccatgatt atcgaacact tggtcgtttc ttgaaagacg
    26041 ctgtagaaaa tccaatcaaa cctgtgaaag tagacaaagc agacgctcca gcacaagaag
    26101 tcgttcatct agctaccgat gcagacttcg atattcgcaa cattttgcct gcacctacaa
    26161 atactcctga agatgctggt ccatacatca caatgggtgt agtcttagga tcagatccag
    26221 aaaaaacaat gagcgatgta acgatccatc gtatggttct agaagataaa gatacgatcg
    26281 gtatgtacat catgcctggc ggacgccaca ttggtcactt ccaaaaacaa ttcgaagcct
    26341 tgaataaacc aatgcctatc actatcaata ttggtttaga tccagcaatt gccattggca
    26401 cgactttcga accacctacc actccacttg gctacaatga actatggtgt gcaggagctt
    26461 tgcgtaacga acctattcaa atcgttgatg gtatagcagt cgatgaagtg ggtattgctc
    26521 gtgctgaatt catcattgaa gcagaaatca tgccaaatga aacaatgcaa gaagatatca
    26581 acacgaactc aggaaaagca atgccagaat ttcctggcta tactggggat gctaatccag
    26641 ctgtacaagt agtgaaagtc aaagcagtta ctcaccgaaa agacaatcca atcatgcaaa
    26701 caacgattgg tcccagtgaa gaacacgtat ctatggccgg tattcctaca gaagcaagta
    26761 ttcttgcctt ggtcgataaa gctattccag gaaaagtagt aaatgtctac aatcctcctg
    26821 caggtggtgg taaattgatg accatcatgc aaatccgtaa agaaaacgaa gcggatgaag
    26881 gtattcagcg tcaagcagct attcttgctt tatcagcctt caaagaattg aagacagtca
    26941 ttttagtcga tgaagatgtt gatattttcg acatgaacga tgtagtttgg actttcaaca
    27001 ctcgtttcca aggagataaa gatatcatcg tgatcccagg aatgaggaac catccattgg
    27061 acccatcaga attaccagaa tataatcctg gctctattcg tttccgtggg ataagtgcaa
    27121 aaactatctt tgacggtaca gttcctttcg atatgaaaga acattttgta cgtgcaaaat
    27181 tccaagaagt agaagattgg gaaaaatatt tgaaataatg aatgatctgc aactgttttg
    27241 acatcataaa aaggagtgcg gaaaatggaa caattggcac aacggaagaa aaaaatcgtg
    27301 attggtgtaa gtggcgcttc aggcaccatt tatgccatca acctagtgaa gaaactaaaa
    27361 gaatatccga tgatagaaac acacggaatc atcagtccat gggcaaaaca gaacttaaag
    27421 ctggaatccg acttatcact aactgaattc gaaagcctgt ttgattacca ttattcaaac
    27481 aaagatttag gtgctgctgt cgccagcgga tcatttttga ccgatggaat gatcatcgta
    27541 ccggcaagta tgaaaacagt cgcaggtatc tctgttggtt taggcgacaa cctgattgca
    27601 cgtgctgctg atgtaacaat gaaggagcaa cgaaagttag tcattgttcc tcgtgaaacg
    27661 ccattaaatg ccattcactt ggaaaacttg actaaattag cacgactagg tgtgcaaatc
    27721 ataccaccaa ttcctgcttt ttataatcag cctcaaacga tccaagatct tgttaatcac
    27781 catacaatga aacttttgga tcaatttgaa attgtaaatg agcattcaaa acgttggcag
    27841 ggtgattaat atggaaaaag aaacgatagt tgaagtaacc aaactaggtt tcacgatcca
    27901 agctgctgtt aaagtaatcg gtaaagactt attgatcact ttaacaggcg gagatacacc
    27961 acatatcgga acagtcacta cattttctcg tgatacagag atgcaaactg tacgtttccc
    28021 tagccacgat ggtcggttcc ataaagacga tgtcttagcc gaaaaaatag ctgtgatcat
    28081 caaaactgcc gttccgggaa gttgtacgat cacttccggt atccatgttg accatatctc
    28141 caatgcacaa atcaacgcct cttttccgat gtcagaagag ttaggttaca agattttaga
    28201 ttggttggaa aaaatagatt tccatatcaa agaaccagtt tattataaag atggtgaaaa
    28261 accattatga aagaagaggt tgtgaaatca gcgttatgac ctgagtatga gagaaaaaaa
    28321 tggaaaaata gcttctcata ttttgccgtt tttgagctaa taccgaggga cagctgattg
    28381 aacgccgttt atcaagaggt tgtgataaag ccgcttcgtt tcgagtagta agaaaaattt
    28441 tgtaaacatg ggtttccaca tttttgcata aatttgactt actaccgaga aactggcttt
    28501 tgaacaccgt ttatcaagag gttgtgacac gtcttttgtc acaacctctt ttatttttgc
    28561 ttcactgtaa ctgctacaat atcaccgatt tcttttccga tgtttttacg aatatctttt
    28621 cgtagtccaa ggatgtagca gactgaacca tctggatttt tcacgcccat attcacgata
    28681 ctgccttcat acggcacatt gtcaaaagtt gcttggactt taactcttcc agcaccgaat
    28741 tcttttctta tatcatacgg aaaaataata tatgccccgc ccttgccgac ttcggaagca
    28801 tagatttttg attcaaaaga ataaatttcc ctcattttat ttctcctatt ttttacatct
    28861 agtatagcat agcaaaaaaa agaaggaact gtgacaaaag tcttgtcgca gttccttttt
    28921 tcgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatag tcaacaaaca
    28981 tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttaaacggca ggctcacaac
    29041 ctcttcctta aacggtgttc aaaagcttgc atctacggca ctaagcccaa tcagatgaaa
    29101 aatatggagt gctattttta tctgattgtt cttactgctt gatgcagagc agctttttca
    29161 caacctccta catggtgttc aaaatattat tcgccttttg cttctttcac taattttgcg
    29221 aatgcttgtt gttggaattc agcagctaag gctttcgtac gttgttcttg ctctgctttg
    29281 ctcatttttg gattttcagt cattgctttt accataccgt cttgaacata agcttttgct
    29341 tcactttcca tcgtactttg ctgtttcttc actaattcag ctaacttggc tgcttggtcc
    29401 gtacttaatt ctacccatgt ctctgggatt ttgaatgtat tcactcggct gtcatattcg
    29461 tgattgttat cagcaatatt gaaccagaat ttatacatgt cgtttttgta ttcccaataa
    29521 gtagttttcg tcactttttc agcatcgccg tctgtctttt ctactttatt tgtttcgtta
    29581 tccgtaccag ttgcttttgg tttttcttgt ttttcatttg ttcggtaaag atacactttt
    29641 tcttttccat tccctaaagc tttatacaaa agcatatctg ctccttggga atcagcactg
    29701 gaaacaattt tttctgtttt tgtttccgtt actttttcca ttccaaagtg attggacaaa
    29761 ttagcgacga tcaaacttag tgaagcgacg aagattagtc cgaaaatcaa agataaaaag
    29821 gtttgccatg tttttttagc gaacacgttc gttaaagcaa aagcaagcaa gctgacaatc
    29881 aaaactccaa taatcatgca ttatcccccg ctttcattag aacaggttct tttttcttca
    29941 ataaaaaggc aataataaag ccaagaatcc caaagatgat cgctacgata aacgcggcat
    30001 ggtaacctga cagtgtggca ttgattgcct gatctttata agctaatggc gtattatcca
    30061 atacagattt gcttgggata tttgcttttg tcacattcgt caacacactg atcaaaatag
    30121 ctgtaccaat cgagcttgcg atttgacgga atgtattatt taccgctgtc ccatgactga
    30181 tcaagttatt tggcaatgca ttcataccag aagtcgttac cggcatcatg accatggaga
    30241 tcccaaacat acggatcgca taaaggatca cgatatataa aactggagtc gtttttgtca
    30301 agaaagcaaa tggtgcagtt gccgctgtca aaatgaacat ccctgtgatg gctaaacgtt
    30361 tcgcgccgta tttatcgaaa atcgcacccg tcacaggcat catagccccc atgatcaatg
    30421 cacccggcaa caacatcaag ccagaatgga atgctgattc tccacgaata ttttgaatat
    30481 acaatggtac gaccatttct gcccctacca tcgccatatt tgttacacca gctaaaacag
    30541 ctgcaatcgt gaatgtagat gatttgaata cacgaagttc caagaatgga tgttccaaat
    30601 gtaattgacg ccatgagaac aaggcgatga aaatcacacc aacaactaag aaaccaatta
    30661 ctttcaagct tccccagccg tcatctccta cactagaaaa tccgtaaagt aaactaccga
    30721 atccaatcgt tgataaaata gcggataaaa tatcaatgct aggatttgac aatggaataa
    30781 cacttttcat taggaagaat gctaaaacca agacaagaac aacgattggc agtaccatac
    30841 caaacaagtc acgccaatgg tagttatcaa tgatataacc agataatgta gggcctaaag
    30901 caggagcaag tccaataacg atccctgaca tccccattgc tgatcctctt ttttctggcg
    30961 ggaaaataga tagcatgata ttttgcaata atggcatcga tactcccaca ccgcaagctt
    31021 ggatcaaacg accgattagt aatgtactaa aattaggtgc tgtaaagcaa gtaatcgttc
    31081 cgatcaaaaa gatcgtcatg gcagaaagat ataaatttct tgaactgaat ttattgatca
    31141 gccatgctgt tattgggatc atgatcccgt tgactagcaa gaaaccagtc gttaaccatt
    31201 gcacatcaga cgctgaaata tcgaaagctt tcatcaatgt aggaaaagct gtcgtcaata
    31261 aggtctgatt caaaacggta caaaatgtac caattaataa gacaacaact aaaagtgtcc
    31321 gattatatgg ttttccgtaa atatctacgg gttggttgtt actcatattt ctccttcttt
    31381 ctgaattttt tctgaaacta ctagaatact ttagtcctaa attcgctatt tgtcaaccta
    31441 cttgacacat aagttaatac ggatataatt aagggaagca agaggacaaa aggagtgaca
    31501 aaaatggttg gggcttccct taaacgtttg ttggaaaatg atcaattggt tgttggcatc
    31561 agtgaattaa gtgatatcgt aggtgtgtct ccacgtcaat taaggtattg ggaacaaaag
    31621 ggattcattc gttcgatcga agctgatgcg aattgtccta gaaaatatcg tttacctaca
    31681 gtcataaaag tagaactgat caaaaaatat ttagatgacg gatttacttt gtcaaaagct
    31741 gcagaaaagg cagaaatccg ccaaaagaaa atgcaccatg tccgtaaagt tttttcaaaa
    31801 gcaatcaaag atatcgaatt gatcgacgaa cgctatacga ttttacaaat cggtaacttc
    31861 aaagaagaga atgctaagat gtacatcatt catgacaacg agacagacga attaacttac
    31921 aagctccttc ctgtcgatga aaaagctgac tacgaacagt tgttaaaaga actctaaacg
    31981 ccactaatct cctaagtacc aagcttttta ataagtagat gctacataaa tacttgaaaa
    32041 aaggcctgtt tgaacataag aatatccgaa cggtgatctg cgtttctttc atatagaaag
    32101 gaattctcca tcactgttcg gatatttttt tatttttttc ggttactttc ttctaataca
    32161 tctgttagcc aatttgcata ttctgtttcc cgattgatcg catgcgctaa aatcaggtag
    32221 tgtccgtaat tttcatgttt ttctttttca gtaggaaaga tcgttttttt tcgctctaat
    32281 agatgaagtc gtttttcttc atgaaggtca cgctgctgct gaataatttc tactaatgcc
    32341 ggatcattca cgtctttgac gaaatacaat ttcagaacaa attcgtctcg attaaccggt
    32401 agttcattgg tgggtgtatg aatccactct gccaattgtt ccttcccttc ttctgttaat
    32461 tcataaactt tcttttccaa tttttcacct gtgatctcta cttgatgctg aatgattcct
    32521 tgctcttcca tttttgcaag ttcaggataa atctgactat gttttgcttg ccaaaattct
    32581 cctatttcat ttttgaacga ttgattcaat tcatatcctg tcatcttctt cttacttaat
    32641 aaccctaaca aaatatatcg aagtgtattt tttcttgcca tcagttttcc ctccttgaca
    32701 aatctttaaa tccattctat cataaatgag taatcgaaaa catgtagttt attacatgtt
    32761 ttaatttata aggagatgct taaacatgaa agaatttaaa gaattggaag attttattgg
    32821 aactcacttt atctatacgt atgacaatgg ttgggaatat gaatggtatg ccaaaaatga
    32881 ccatactgtt gattaccgta tccatggcgg aatggtcaaa ggccgttggg taaaagacca
    32941 agaagctgat atcgttaaat tgacggacgg cgtattcaaa atcacttgga cagagccaac
    33001 agggacagac gtggcattgg actttatgcc aaatgaaaag aaacttcacg gaatgatctt
    33061 tttccctaaa tgggttgaag aacatccaga aatcactgtt actttccaaa acgaacatat
    33121 cgcagaaatg gaagcagctc gtgaaaaata tgaaacttat ccaaaattac ttgttcctga
    33181 atttgctacg atcacttata tgggtgatgc aggtatagac aatgaagatg ttattagcga
    33241 agaaccatac gatggaatgc cagatgatat ccgtagcgga aaatacttcg atgaaaatta
    33301 taaacgagta aaataaggtt gtgaaagaga ggttgtgaaa tcagcgttct gacctgagta
    33361 ttagagaaag aaacggaaaa atagttcctc atgttttgcc gttttttgag ctaataccga
    33421 gggtcagctg attgaacacc gtttatcaag aggttgtgaa atcagcgtta tgctttgagt
    33481 aataagaacg ctagttagaa aatagctttt catattttct aggctagtgg gcttattacc
    33541 gagaagcagc cgtttgaaca ccgtttatca agaggttgtg aaatcagcgt tttgacctga
    33601 gtattagaga aacaaatgga aaaatagttc ctcatatttt gccgtttttt gagctaatac
    33661 cgagggtcag ctgattgaac accgtttatc aaagattgta acaactgcat attcacacag
    33721 ttaaattagt gacttcaaaa tgaaataatg ctcccagcaa aaaattaggc tgggagcatt
    33781 atttcgttat tctgttcatc aaaaatcagc gttttgattc catttcatca caagttcctc
    33841 ctcatttact gcttcatctt tttgctgtgt ttgaattgta aaaccttgtg aaagatagaa
    33901 atgaatagct gattgatttt tttgatagac tgtcaaagtc agtgttttat tggtttcttt
    33961 tatttttttc attagttgcg taccgattct ttgttgccgg tattcccttt tcacaaagat
    34021 tcctgcgata aactgatcaa tcaccccagc aaaaccaata atttcttgat ctatttctgc
    34081 taagtaaatc actgcatctg gtaaagcttt cctaacagat tctacttgat tctcccaata
    34141 agttgatgga atgaattcat gagaatccaa gttagcatgc agccaaatcg atagaacaga
    34201 ttccagttct ttggcagaca attttgttgt tgtgcggatc attttttctc tccttactta
    34261 ttcattgatt ttaaaataaa aaccattttt ttgaagctct tttacaagtt ttcgcccctc
    34321 aaaaaagtat atcttctctt gttttacttt tctaatacca aaagaaacag caactttagg
    34381 atcatcagct gaaacgattc gataagttcc accaggcaat tgatccagct tgactttccc
    34441 atgatggtcg ctttgtactg aaaacggcac accatttcta gtaagtgttt ttttcttatg
    34501 tttcaaaatg aatgaactac catttaccgg tttccctgac gaatcactaa taataaatga
    34561 aaaatcaagc acttttttac ttaatctata ctgatagatt agtcgtccga taaacactaa
    34621 cagtatgact acagctatta tcgttcctgc aagaataaac cattgccgca tggttctttc
    34681 ctgatttcct tttccttctt ctttttttac cgattcgtta taaggaatcc gatgtcctgt
    34741 cactagcaaa cgatgcgtgt tgatcatata cggcgtacat gtaagcaagg taactaagtc
    34801 ttctccttct gaaatcgtta gaacagacgt gtcatctggc agtaccactt gtatattttt
    34861 aacttgataa gccaatttct cccccaaaac agtcaaaata aacgtgtctc cgttttctaa
    34921 acgatctaat tcggtaaata accgtcgatt aggtaaaccg ctgtgagcag agatcactga
    34981 atgcgtcgaa tttccaccaa gcgggaaaga ggttccttgc aagacgcctg ctccattttc
    35041 aaggatccta ccattgagcc gatcataaag tgaaatattt aattggatct taggtatatt
    35101 gatacttccg atcagccatt cctctaattg gctggatgtt gtactttcat ttctgttctt
    35161 ttcattgaaa ggatcagtac caggattgaa tccttggtca gttaatcgtt ggtttttctc
    35221 ctccattttc tttcgctgca cttcaatttc agctgcggtc cgtttttgta cttgttcgat
    35281 acgtttttga tcaataaaac tatttaaaga atccacataa aatggatata aagcgatcag
    35341 taaccctacg acgatacata ttaataaaaa caaacgaaac aaaagtcgac gagactgttt
    35401 ttgcttactc tgcttcacat gtgtcctccc ttgcaagttt ttttgttctt tcttttatta
    35461 tatgggttca ggaaaaaaat agaaaatgaa agccgatgag gttgtgaaaa agctgtcctg
    35521 catcaagtaa taagtacagc ccaaaaaaat agctactcat attttttgtt gggttgggct
    35581 tattaccgca gatgcaagct tttgaacacc gtttaaggaa gaggttgtga aaactgcgtt
    35641 atgctttgag taataagagc gctagtcaga aaatagcttc tcatattttc taggctagtg
    35701 agcttattac cgagaagcag ctgattgaac accgtttacc gagaggtcat gaaaaagcta
    35761 ctttgtttcg agtattaaga aaaattctgt ttcaaaacaa ggagatgcaa caaaacttat
    35821 gtcgcatcct ctctccttag aactttcact tatgtttttc ttgtaaaatt cataaatatt
    35881 ttcatgaatt ttctaatttc ttgcctacct ctcgcatagg atatatgatg aatttatcta
    35941 aagtttgcaa aggagttcga caattgaaaa aatatggccc atttttagca atttttttca
    36001 ttgctttggc atttctcatg cctcatttat tcacacataa actaattttg ggtgctgatg
    36061 ccccattcca ttacaatcgg ttctatgatg ccgcgcaaca aattaaatat ggggatttcc
    36121 attattttcc ttccctttat ggattccagc aaagtggcag gatcgtgaat gccgtttacg
    36181 gcccattttt cgcttattta caaggattga tcgtgttgct tgcaggtagc tggtttcatt
    36241 atcagattct ttcaaatttc attctttatc ttatttcagg attttctatg ctgattttac
    36301 tgagatatgt caatgttcga aattgggtaa gtgtaccagt agccgtttta ttcatgagta
    36361 cgtattctat tcaatactgg tctatcaacc aaggattcac tagctggggg acttgttttt
    36421 atcctttatg tatgatccct attgctgatt tcgtccttca taaaaagttc cctgttatca
    36481 aagtcgctgt ctcagtggga cttatgacac agatccattt actgtcttcg atcatgttag
    36541 ctttgatgta tatccctttt tatctttatt acttttttaa ccagactgaa aaaggaaaag
    36601 cactgcttca actgattaag tcggtcttgc tttattttgt tctgacagca aatatttggg
    36661 caggattagc tattgtttac agtgggaata agattcttcc tccctttgtt aacagagaaa
    36721 tgagtcaaaa ggcgatagat ctggatggca gttattggct ccattaccct aaggcctttc
    36781 ctatattgat attatcaggt tttttgcttt ttattttctt tcaacaaaag aacaccttat
    36841 tccaaaaaat cgtgttgtac acaagtcttg ttttcttttt tctttctact agcttgattc
    36901 catggacgac attgatcgaa aaaaatgtgc cttttattag tttgatccag tttccatttc
    36961 gatttttcgt gccatttacg ctattgcttc tactttacct tgccctttca ctgaaccagt
    37021 ggtctgaaca aaagtggttc cgtcttttaa gtatatttgg cattcttatt ttttcagcac
    37081 agacgatcca gaacttatac cagcatctgg aaaagtggga aaatgaaaca tttgtcagtc
    37141 gacatactta tctattcgat acgccggaag aagcaaggaa aacattcttc agcaaagata
    37201 tgtctaccag cttgttcgtt ttccaaaaaa ccacacctga ctatctgcca atctataagg
    37261 agacaaaaga caataaatac gaccggtata acgaagaaat actagaaaac gaaacaactt
    37321 ttataaaaac acatccaggc ggtcttttga caatcaagtg gaaaaacact ggggacaaaa
    37381 ctgttcatat ccctgttatc gtctacgatc ggactgtgct acagcaaaat ggaaaaacat
    37441 tgactgatta tgaagtaaca gatatcggga ccccaatcgt gaagcagcaa aaaggaatca
    37501 acgagctcac gcttcattat caaacgccaa tctatttcta tttcattctt tctcttactt
    37561 tgattggctg gtttaccttg ctatgtttat ttatttatca tagatacaag cttttacgtg
    37621 cttaggcaaa agggataaat acatccgatc aaaaaactct ccaaacggta taatcgtttg
    37681 gagagtttta ttttaatcag tttttttatg ttatatgtta tattttttat tcgttcacat
    37741 ctaattcgat ttccaccccg tagtgatcac tgatgattgg actatcgatc ccatcaaaaa
    37801 tccgttgata ttttttaacc tgcattcctt cagggacgaa aataaaatct atacgcaatt
    37861 tttctgtatt ttgttcccaa ccatctattt tcttttccac agtagctgct tttccttttt
    37921 cattcgcgac aacatacgaa tcttgaagaa gaaggcaact ttctcttaca gtataataac
    37981 cttgagagtc tactggatta ttgaaatcac ctaggaaaaa aagtggtacg cgagtttcca
    38041 aaaagtagtt ttccaagttt ttccactcaa aataaaagcc acttttttta tctgtccacc
    38101 aagaaaagtg gcagctcgag acaaaaatgt cctgatcatc tatctttgtt ttcccaatca
    38161 agatcttgcg tgtctgataa tcttctttct tttgaaattg ggagacggta aggacctgac
    38221 tttccaatgt ttttttcgta agcagtgcat ttccttcttc atatttatca taaccgatgt
    38281 ggctcatttc ccaactccaa aaatactctt ttccgtgttc ctttaaaagc tgaactaaac
    38341 ggtaggcaaa attatcttta cggataggta cttgattctt taccggacag aacttcatta
    38401 gcttcgcagg ttctacttct tcagattcca gtaattggtt gatttcttgt aaagcaatca
    38461 cttcataatc ttctaacagg atcttatctg ctaattcttg caattttttc tctggttctg
    38521 gctcaagcca gctatgcgtg ttgagtgtca tgaatttcat tggttttctc cttgtcttca
    38581 tttcgaaaca aaagggttgt gacaaaattt ttgtcacaac ctctccagca aatagtgttc
    38641 tcctgctaac tcctcagtaa taagctcaat ggaccaggag ctattttttg tttggctgtt
    38701 tttatcactt ggtgcagggc agctttttca cgacctctcc actaaacagt attcaaactg
    38761 ctgcttctcg gtaataagcc caaccaaacg aaaaatagga ggagctattt ttgtttggct
    38821 gttcttatga cttgatgcag gacagctttt tcacaacctc tccttacatt ttaatggagc
    38881 cgatcacttt accagctgtc actgtaccta attgcttgat cactacttct tctactttat
    38941 cgtttgtaaa tacgatcatg atcgttggat ctttttcttc gttttggatc acatcaagat
    39001 ccatctcagc taatttcgtt cctgttccta cttcttggcc ttctttgaca gcaatctcaa
    39061 aagctggctg attcatttgg actgtatcga ttcccatatg gatcaaaact tcgataccat
    39121 ctttcgtttc cagcccaatt gcatgtttgc tagggaaaat acttgtcact tttccagcga
    39181 tcggcgcaac aacttctctt gatgttggaa taacagcaaa gccgtcaccc atcatttttt
    39241 gtgagaatac cggatctttt acttcagata aaggaatcac ttctccagaa gctgcggaaa
    39301 ctagttcttg ttctatacct aagtattgct tactacctgc ttgtccattt cctgtagaaa
    39361 ccatttctgg ctctggaatc tctgctcctg attgcagcag atcttcgata tctgatttca
    39421 agacatctgc ttttggtcca taaactgctt ggactccatt gtctttgacg atcaatccca
    39481 tcgcccctgc tcgtttccag gcttcttcag tccctacttt ttctcgatca tttacactta
    39541 cgcgtaaccg agtcatacaa gcatcgacat ctttgatatt ttcttttcct cccagcaaat
    39601 gaatgatctg gaccacttgc tgatctgctg ttcctgttcc gctgttagat acaacggttt
    39661 cttcactgtc attgtcgtaa tttccgttac gtcccggtgt agcaaagttg aattttttga
    39721 tcaagaaatt tgctaagaaa tacgtaacaa caccaaaagc gatcacacac aacacgaagt
    39781 tgataagatc tccacctaga cccgctttga tagctaacgg tgtccgagtc agcaattcga
    39841 tattcccaaa tgaatggacc cgcaatggca agatatctgc catcgcaaat gcagcccctt
    39901 ggatcactga ataaatgaca tataatggca cagcagcaaa cataaacatg aactccaatg
    39961 gttctgttac acctgttaga aagacagcca acgctgctga aaaatacatt gatttatatc
    40021 ttgctttctt atcaggatcg acattacgat acatcgcaaa agccattccc atcaagatac
    40081 ctgaagaacc aatcatttgt cctactttga aacgagccgg cgtccagtta tgcaacacaa
    40141 actcatattg cgacatatcc cctgcacctt taagattcac taaatcagtg gcccaagcca
    40201 accataacgg atcttgccca aatacttgtg tccccgcctg tgcgcctgat aaaatctcat
    40261 acgatccgcc aagctgtgta tagttgatag gaatcgtcag catatgatgc aatccaaatg
    40321 gcaatagtaa acgttctaat gttccaaata aaaacggtgc tagaatcggt gcgctttctt
    40381 gtgattctgc aatccataaa ccaaaattat tgattccagc ctgtacgttt ggccacacca
    40441 cagcaagaat caatgccacg attgttgacc ataagataac aacaaacgga acaaagcgct
    40501 ttccattgaa aaatgacaaa gcatccggca attttctata attatagtac ttattataag
    40561 ccattgcccc gacaaatcca gcgatgatcc ctacgaatac tcccatattc aaagctggtg
    40621 cttccaatac acttgtaaaa aatcctttaa ccatgatctt cgtaccgaac agagtgtggg
    40681 taaatgcatc ttcattcgca agcatgtcac ttgtgacacc aaagatagcc ccagtgatcc
    40741 gattgattaa aataaatgag atcccagctg caaatgctcc gcctgctctt tcttttgccc
    40801 aacttcctcc tattgctaaa gcaaataaaa tatgaagatt accaataatt gcccaaccaa
    40861 tattttcaat tactccgccc gttgtgacaa gcggcgtcca atcaggattg atcaagggaa
    40921 ttgatttccc tatactgatc atcaacccag ctgccggcat aactgctacc acaaccatta
    40981 aagcctttcc aaatttttgc caaaactcaa aactaaatat ctttttcatt ccgtttagct
    41041 cctttacgca atcggtttcg ttaaacgtat tatagcgtgc gttttctttt ttgtaaatgc
    41101 tttatttttt atttttctgt ctataccatt ctataaaacc tataatacgt gtgttccctt
    41161 ctataatttt ctcctaaaaa tttatttttt taagcttttt tctatgaaat attttctttt
    41221 gtctattgac ttgtgcaatc ggttgcgttt attatgaacg tataaaacag ctcattagga
    41281 ggagaaaaat gaaacagatc aaacgactat ttgctattga tccatggaaa atcagcacga
    41341 cacaattaga taaaaaaaat cttcgactgc aagaatcttt aacaagtatt ggaaacggtt
    41401 atatggggat gcgcgggaac tttgaagaaa cttactcagg ggaccaccat caaggtactt
    41461 atttggcggg tgtctggtat cccgataaga cacgtgtcgg ctggtggaaa aatggttatc
    41521 cagaatactt tggaaaagtg atcaacgcct tgaactttat cgcgatagat atttcgatca
    41581 acgatcagct gatcgacttg gcttctttgg aaccagaaga cttttactgg gaattagata
    41641 tgaaaaatgg tgtattatct cgtacattca caatcactac ttctactaat aaagtacgca
    41701 tttccttcga acgttttctg agcattgtca aaaaagaatc agcttatatc tatttaacta
    41761 tagaaatgct tgaaggtagc ggaacagtca aactcgtatc aaaattggat ggaaatgttc
    41821 aaaacgaaga cagcaactat gatgaacatt tttggaatga gattgatcgt ggaactatag
    41881 aagaaatcgg ctatttgacg actaaaacta ttccaaacaa ctttgggatt gaacaattta
    41941 ctgttactgc agcaatgcgc catttcgtta atggaaaatc agtcatccct gactatcacg
    42001 aagatacttt agcattacat ggttgcttgt ctgcagaatt atctcaaggt gagacatggt
    42061 cagtggaaaa agaaattatc gttcttacta gtagagatat tccagaagcg cagcaagcag
    42121 aaaaagctgt ctcagaatta acgaaccaga caaacaatta tatccaagca aaagaagagc
    42181 aaacagcggc ttgggcagaa cgatggaagc ttgccgatgt ggtcatcgaa ggagacgaag
    42241 aagcacaaca aggtattcga ttcaatttgt tccaactatt ctctacttac tacggcgaag
    42301 atgaacgatt gaatatcggc cccaaaggat tcacaggaga aaaatacggc ggagccactt
    42361 actgggatac agaagcgtat gctgtccctc tttatcttgc tttagccaaa cctgaagtaa
    42421 ctaagaatct gctgaaatat cggcataatc aactgccgca agcaatccac aacgctcagc
    42481 aacaaggatt aaaaggcgcg ctttatccta tggtcacctt cactggtgtc gaatgccata
    42541 acgagtggga aatcacattc gaggagattc accgaaatgg cgcgattgcc tatgcaatct
    42601 acaattatac gaattacacc ggcgatacgg aatatttgaa aaatgaagga ctggaagttt
    42661 tagcagaaat cgcacgcttc tgggcagacc gtgtccattt ttcaaaacga gcaaataaat
    42721 acatgatcca cggtgtgacc ggcccaaacg aatatgagaa caatatcaat aacaattggt
    42781 atacaaatac gatcgcagct tgggtcttgc gttacacgcg agaaaattat ttaacttatc
    42841 aaaaagaagc tgctatcact atcagcgaag aagaactagc caaatggaaa gatatcgcag
    42901 agaacatgta cttcccagtt gacgaagaat taggaatctt tgtccagcat gacacattct
    42961 tagataaaga tctcatgcct gtatccgata ttcccacaag cgatcttcct ctgaatcagc
    43021 attggtcttg ggacaaaatc cttagatctt gcttcatcaa gcaggcagat gtactacagg
    43081 ggatttattt cttcaatgat acttttacaa aagaagaaaa gcaaaaaaac tttgatttct
    43141 atgaaccaat gactgtccat gagtcttccc tttcgccaag tatccattcg attttagcag
    43201 cagaattagg aatggaaagc aaagctgtag aaatgtacca gcgaacagct cgcttggatt
    43261 tggataatta caataacgac acagaagacg gcttgcacat cacttccatg actggtagct
    43321 ggttgtcgat tgtccaagga tttgctcaaa tgaaaactgc ccatgatcag ttgagattcg
    43381 caccatttct gccaaaaact tggaaagctt attctttcca tatcaattat cgcggacgtt
    43441 tattgttcat ctcagtcaca gatgaacaag ttactttcga gttattagaa ggttctcctc
    43501 tagaaattta cgtctatgaa cagtcttatt ctttagcgga tcgtctagaa ataacgctaa
    43561 gaaaggagga agcaaatgtt taaaggggta ttgtttgatt tagacggtgt cattaccgat
    43621 actgctgaat tccattatcg tgcttggcga aagcttggtc aggaaatcgg cattacgatc
    43681 gaccacacat tcaacgaaca gctaaaaggc gtcagccgag aagactcact ctctttgctt
    43741 ttagcttatg gcggaaaaga acactctttt tcaaaggaag aatttgcaga attagcgaaa
    43801 agaaaaaacg attattattt agagatgatc cagacgatcg aaccaaaaga tgtcttcccc
    43861 ggggtcgtgc ctttactgga ttctttaaaa gaagaaaaaa tcaagattgc tcttgcttct
    43921 gcaagtaaaa acgggccatt cctacttgaa aaaatgggtc ttacacctta ctttgatgct
    43981 attgccgacc cggcagaagc aacaaacgga aaacctgcgc cagatatctt cctccttgca
    44041 gcaaaagctg tcggactaaa agcagaagaa tgtatcggga tcgaagacgc gcaagccggt
    44101 atccaagcca tcctctcaag tggcgctcaa ccaattggcg tgggacgtgc tgaagacctt
    44161 ggagaggaga tcccgcttgt ccctgatacg acttttctta cgatcgatta cttgaaagaa
    44221 aagtggcatg accatggata atccagagat ctccgtcgta ccagttccag agactgaaat
    44281 aaagaaaatc acattgaaga acgactactt agaagtcgtt cttctcaatt acggtgcccg
    44341 cctgcaccaa atcttcgcac cagacaaaga aggaaaatca gaaaatgttt tattgtctta
    44401 cgatcgtttc gaagatgttc taacagacaa aagctttttt ggagcaactg tcggacctgt
    44461 agccggaagg atccgggatg cttcttgggg tgagcataga ttggagaaaa actgcggatc
    44521 acaccatatc cacggtggca cgaaaggctg gtctttccaa ttttgggaag tagaagtttt
    44581 caaaactcca tcatcaatag gtgtggtctt ttacttaaaa gatgaatttt ctacctatcc
    44641 aggaccaatc actgctacga tcacttaccg tttaacaaaa aacgaattag agatgacaac
    44701 ctctgctagt agccttgttg aaaccatctg caatccgacc aaccatgcct acttcaacct
    44761 ttcaggaaat ggaacaagag acatctatga acatcagtta acggtctttt tagatggtgt
    44821 gcttgaatta gatcaagaaa aactgcctac tggaaattgg acaaaaaaag aaaaactgcc
    44881 aatcgacttt agaaaatccc caacactgca agagatcctt gcatgttatc cagatggact
    44941 agatgatgta tttcttttgc atcatcctag gctttccaaa acttccttac aattattcga
    45001 gaagcattct ggtcgccaaa tgactatagc aacttctaat aaaagtatgg tattattttc
    45061 tacgactgga ttcgaagctg atttctctgt caatggaaaa cagatgcatt caaactacgg
    45121 attagcaatc gaaccgcaag aaatccctga tatcgttcat ttccccaaat ttggatcgat
    45181 caacctccat cctggtcaag aacggatcag tcagacgatt tatcggttct ctgcacaata
    45241 acaagagaaa ggtgttttta tcatggctat taccgtaaaa gatgtggcga aaaaagccgg
    45301 tgttgccacc tcgaccgtct ctcgtgtcat caacgatcat cctagtattt ccgagacaac
    45361 aaagaagaaa gtccgaaaaa ttatggatga actaggttat gttcctaaca tgactgcccg
    45421 caatctagga aaacgcattt ccagtgccat cggcgtaatc ttaccccctc ttgattcaaa
    45481 agagcggatc ggtaacccat tttatcttga gatcatggaa gcaatcaacg aagaagcgcg
    45541 gaattatgat atgactacag caatcgctac tgctaaaaat tttgaattgc tgttagagaa
    45601 cgtccagcgt atgcatttac aaaaacaagt agatggtttt attctagtgt attcagatag
    45661 tcaagatact gtcattgatt atttatacca aaaccacatc cctttcactt tgatcggaca
    45721 gccttatcat catgagaacg aaatcgttta tgtagataat gataaccagc ttctaggtaa
    45781 acaagcaaca gaatttttga tccagaatgg acataagaat atcctgtttg tgacaaacac
    45841 gactcatgaa agtttgtatt tcgaacgata tttcggttat caaaaagcaa tgatgatggc
    45901 tggtcttcct gcgttatcca gtgtagtact agagcatccg gaggattatc tgtcctttga
    45961 gaaaacgttg aaagaaaacg aagccacagc attggtggtc attgacgata tcttcgccct
    46021 tcgcacgatg cagttggcgc aaatgtatgg ctatgtcatt ccagagacac tctccgtcat
    46081 tagcttcaac aactcgatct tctcaacatt aacacaccct taccttacaa gtatcgatat
    46141 cgatatctcc gaattaggac gtgtcgctat gcaaaagctg cgcgaattga tccatgaaga
    46201 attgagcagt ggcgtacgtt tggtcatccc tcatcgcttg atcaaaagag agacagtcgt
    46261 tcctttagaa aacccatact aaatagctga ctcactaaat acgctgacca tttatcctat
    46321 ggtcagcttt ttatgtaaaa gaagatacta cttctaacac attgtttatc tgtaaaaaag
    46381 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
    46441 aagcagaagc aactccttcg gaaataagct gaaattcaca aaaatttaaa aagcaatttt
    46501 cttgagtttc ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttccttc
    46561 cttcaatttt ttacacgtcg acgattcaat aaaaaaccgc ctaatattgt aaagacaggt
    46621 gcaagaatcg agaaaaaggc gaatggcaag taatgcaacg cgctaacttt catcgttccc
    46681 ataatgaatg tgccgctgac tccccacggt acgagagaat tgacggtcgc acctgaatcc
    46741 gctagtgttc gtgtcaaata ggtttttgat aagccaacgt catcaaaaga acgcttgaat
    46801 gtttctcctg gaagaatgat ggacagatac tgttctccaa cgatcagatt gattccgata
    46861 cagctcaagg cagtaaaccc gactaaacga gaaggtcggt ccatcttttc ttttaattct
    46921 tgaacgatgg tatcaacgat cagatatttg atcagtaacc cacctaatgc caatgcaagt
    46981 aaaatcaaac tagcagaacc taacatactc atgatcccgc ctcgagaaag cagttcatct
    47041 agctttggat caggtgtatt tgcgacataa ccattcatca agatgtctgc tgcttttccg
    47101 accgtcaaac ttttctcatg gataaaagaa agaacgagag cagctgtcga tcccgctagt
    47161 aatgtaggga tagccggaat ttttttgatt gcccccccaa ataaaataac tagtggaatc
    47221 aatgtccaag gagagatcca aaagctcgct ttcatgtttt ggatcatttc attgatacta
    47281 gctagatcgg ctgaatgact gttatgtcct aatatcgtat aaaaaatcaa cgaaaggacg
    47341 aatgctggga aatccgtata acgcatacca gcgatatgtt cgaatagatt aactcctcca
    47401 atacccgctg ccaagttggt cgtatctgat aaaggcgagc agttattccc taaaaaggca
    47461 ccggaaacga ctgcgcctgt agtcattgca tcattgaatc caagtaaatg accgatcccc
    47521 aaaaaagcaa ttccaatcgt cgaaatcgtt gtaaatgaac tgccgacagt cactccgaca
    47581 attgcacaaa caagaaatgc tgtcggtaaa aagaaacgca cagaaacaag tttaaatcca
    47641 taaaccataa tcgtagggat ggttccagat aaaatccatg aagcaactaa gacaccaatc
    47701 aaaagaaaaa tcaggattgg aatgatccct ggagtgattc ctttgatgat cccgtcatgg
    47761 atctcatccc atgtgaactt tttaaatctg ccataaaaca acaaacacat gaaagctgca
    47821 aggatcggga tatgtggcga gagctggaat ccgatgatca gtactcctag gagtgccaac
    47881 aacacgatca atacagcgat actttctttt aaagaaatat ttttattcat cgtcgatctc
    47941 ctatttcaaa tgaaaaggcc ctgccatgaa aaaactattc acttggcagg acctcctact
    48001 aaatcagcga caaaacgtcg gctgaaagac ttattttttt atttttcttc taaacttttt
    48061 tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg aataacgaac
    48121 atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt acctaatgta
    48181 gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca agctaatccg
    48241 tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt acgcatttta
    48301 agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt agcatttgta
    48361 agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc aaataatgaa
    48421 ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt ttctttatgg
    48481 aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt ttttgatggt
    48541 ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa gctgaagata
    48601 tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg ctgcgtgata
    48661 tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc attgatgaca
    48721 ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt ttttgttgcg
    48781 tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat aaatgtgata
    48841 ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt tgaccatgaa
    48901 aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa aacacctaaa
    48961 acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt cccgccgact
    49021 tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac taaaatacaa
    49081 cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt tcctaacaat
    49141 ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca taatagccat
    49201 gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg aggaccgcct
    49261 tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc ggcaaaaaac
    49321 aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa cgtaggaata
    49381 ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag acccatttta
    49441 cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa aataacggtg
    49501 tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag gggattcttt
    49561 gagtttattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga tcgaagagct
    49621 taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt attcaagctg
    49681 ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg tctgcgtaga
    49741 ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct tcaaagtgtt
    49801 cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact tttccggcac
    49861 ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg ctgttgccat
    49921 agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa atgcttcctt
    49981 tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca actaagtcat
    50041 cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa gttaatggat
    50101 gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag ttgtagaaac
    50161 gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca ttcaaaggta
    50221 agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa ccttccaaga
    50281 tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat gtcgcaaagt
    50341 aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca gaatatggaa
    50401 catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt gctttatatg
    50461 cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg tatttgtaat
    50521 gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag aaaatcgcac
    50581 ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg tctttttcta
    50641 atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct tctgttgatc
    50701 ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca cgaacgattt
    50761 tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga atcacttggt
    50821 ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta gtttgtggaa
    50881 ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga gctttgattt
    50941 cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt gacaagttca
    51001 ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc attgcaagcg
    51061 gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa gaaccatcag
    51121 caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc attcctactt
    51181 cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta ccattccata
    51241 gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc atatgacccc
    51301 agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct gaaatttctg
    51361 atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt ttttcttctg
    51421 gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa tgttcatcca
    51481 ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc ccaatgaata
    51541 gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc tcctaaaaaa
    51601 atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta aactaagttt
    51661 tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt cacacttttg
    51721 aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa gattgtatta
    51781 acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt aatagtattt
    51841 ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca aaaagtaatt
    51901 tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg gatttatgac
    51961 aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg aaacatcttc
    52021 acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca cgatattttc
    52081 aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga tttcttccgc
    52141 atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg cttctttcaa
    52201 gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt gtgcttcacg
    52261 tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt gagacaagaa
    52321 agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc agaattggta
    52381 gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg cagtttttcc
    52441 gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg cttctgcttc
    52501 tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc attggtctgc
    52561 tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt cgatagattg
    52621 caggatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc tggcaacgat
    52681 atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca aaaattcaag
    52741 taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg catctttacc
    52801 aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt gttccatcgt
    52861 ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg ttccgccgcc
    52921 aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca taaatggaat
    52981 caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa gtgaaatgct
    53041 cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa tggcgtcgcg
    53101 ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt tttatgaaag
    53161 caggaacctg ctagcatatg taattaatta tttcttcagg ataggatagg aaataaaaaa
    53221 agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat ttgtatgtct
    53281 agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac tgccaaagac
    53341 tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc agctttctga
    53401 aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt aatatgtgtc
    53461 tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt taaattcctc
    53521 attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt attgttaaaa
    53581 gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta ctttgcctct
    53641 tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa gcaagggacg
    53701 aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt aactatcgtt
    53761 attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga accaatcatt
    53821 cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca acaactagtt
    53881 actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc gttattggaa
    53941 aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact aaaaaaggag
    54001 aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt tttccacagc
    54061 ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata caaagattat
    54121 atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa tgattttgtt
    54181 ttagttaatt tgaatgattt gatccaaaaa gacgaacaag gaaatctttc ctttaacggg
    54241 cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt cagctattat
    54301 gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa caatgaaatc
    54361 aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat ggttcctctg
    54421 atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa aggtaccttg
    54481 gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca atatggagac
    54541 aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca gttgaagaaa
    54601 gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca agcttcacta
    54661 gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat cctaggaaaa
    54721 acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc ttattcagca
    54781 gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg taatgtcgat
    54841 gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc tcggatcaat
    54901 atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct tgatccattc
    54961 atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt ttacgtcatt
    55021 tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt tatgctttat
    55081 ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt atatggaagc
    55141 agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa atagaggatc
    55201 aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg atggaaaaga
    55261 tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga aaattctttt
    55321 ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt cactcgattg
    55381 agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg aagattttcc
    55441 gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa tacttcataa
    55501 atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac ctgagcgttc
    55561 tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat caagtcaaaa
    55621 gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc gttcatttgt
    55681 agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat tc
//
LOCUS       DAZKNH010000006        52819 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000006 DAZKNH010000000
VERSION     DAZKNH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.006"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..1119
                     /locus_tag="ACG8MG_000344"
     CDS             118..1119
                     /locus_tag="ACG8MG_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFQ0374423.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQSMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            1138..2157
                     /locus_tag="ACG8MG_000345"
     CDS             1138..2157
                     /locus_tag="ACG8MG_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFQ0374424.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(2259..2975)
                     /locus_tag="ACG8MG_000346"
     CDS             complement(2259..2975)
                     /locus_tag="ACG8MG_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0374425.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(2944..3729)
                     /locus_tag="ACG8MG_000347"
     CDS             complement(2944..3729)
                     /locus_tag="ACG8MG_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0374426.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3720..4469)
                     /locus_tag="ACG8MG_000348"
     CDS             complement(3720..4469)
                     /locus_tag="ACG8MG_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0374427.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4767..5291
                     /locus_tag="ACG8MG_000349"
     CDS             4767..5291
                     /locus_tag="ACG8MG_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFQ0374428.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5307..6806
                     /locus_tag="ACG8MG_000350"
     CDS             5307..6806
                     /locus_tag="ACG8MG_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFQ0374429.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6894..7496)
                     /locus_tag="ACG8MG_000351"
     CDS             complement(6894..7496)
                     /locus_tag="ACG8MG_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0374430.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7600..8556
                     /locus_tag="ACG8MG_000352"
     CDS             7600..8556
                     /locus_tag="ACG8MG_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFQ0374431.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8861..9817)
                     /locus_tag="ACG8MG_000353"
     CDS             complement(8861..9817)
                     /locus_tag="ACG8MG_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0374432.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            9991..10551
                     /locus_tag="ACG8MG_000354"
     CDS             9991..10551
                     /locus_tag="ACG8MG_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374433.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10544..11368
                     /locus_tag="ACG8MG_000355"
     CDS             10544..11368
                     /locus_tag="ACG8MG_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFQ0374434.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11378..11995
                     /locus_tag="ACG8MG_000356"
     CDS             11378..11995
                     /locus_tag="ACG8MG_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0374435.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12013..12696
                     /locus_tag="ACG8MG_000357"
     CDS             12013..12696
                     /locus_tag="ACG8MG_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0374436.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13379..14662
                     /locus_tag="ACG8MG_000358"
     CDS             13379..14662
                     /locus_tag="ACG8MG_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0374437.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            14678..15565
                     /locus_tag="ACG8MG_000359"
     CDS             14678..15565
                     /locus_tag="ACG8MG_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0374438.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15582..16418
                     /locus_tag="ACG8MG_000360"
     CDS             15582..16418
                     /locus_tag="ACG8MG_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0374439.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16421..17044
                     /locus_tag="ACG8MG_000361"
     CDS             16421..17044
                     /locus_tag="ACG8MG_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374440.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            17041..18333
                     /locus_tag="ACG8MG_000362"
     CDS             17041..18333
                     /locus_tag="ACG8MG_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0374441.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18692..18789
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18905..19486
                     /locus_tag="ACG8MG_000363"
     CDS             18905..19486
                     /locus_tag="ACG8MG_000363"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFQ0374442.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19489..20796
                     /locus_tag="ACG8MG_000364"
     CDS             19489..20796
                     /locus_tag="ACG8MG_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFQ0374443.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21212..21700
                     /gene="purE"
                     /locus_tag="ACG8MG_000365"
     CDS             21212..21700
                     /gene="purE"
                     /locus_tag="ACG8MG_000365"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFQ0374444.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21693..22817
                     /gene="purK"
                     /locus_tag="ACG8MG_000366"
     CDS             21693..22817
                     /gene="purK"
                     /locus_tag="ACG8MG_000366"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFQ0374445.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22830..24125
                     /gene="purB"
                     /locus_tag="ACG8MG_000367"
     CDS             22830..24125
                     /gene="purB"
                     /locus_tag="ACG8MG_000367"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFQ0374446.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24247..25635)
                     /gene="ltrA"
                     /locus_tag="ACG8MG_000368"
     CDS             complement(24247..25635)
                     /gene="ltrA"
                     /locus_tag="ACG8MG_000368"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0374447.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(26204..26581)
                     /locus_tag="ACG8MG_000369"
     CDS             complement(26204..26581)
                     /locus_tag="ACG8MG_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374448.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            26837..27565
                     /gene="purC"
                     /locus_tag="ACG8MG_000370"
     CDS             26837..27565
                     /gene="purC"
                     /locus_tag="ACG8MG_000370"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFQ0374449.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            27565..27819
                     /gene="purS"
                     /locus_tag="ACG8MG_000371"
     CDS             27565..27819
                     /gene="purS"
                     /locus_tag="ACG8MG_000371"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFQ0374450.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            27821..28492
                     /gene="purQ"
                     /locus_tag="ACG8MG_000372"
     CDS             27821..28492
                     /gene="purQ"
                     /locus_tag="ACG8MG_000372"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFQ0374451.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            28493..30715
                     /gene="purL"
                     /locus_tag="ACG8MG_000373"
     CDS             28493..30715
                     /gene="purL"
                     /locus_tag="ACG8MG_000373"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFQ0374452.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            30700..32139
                     /gene="purF"
                     /locus_tag="ACG8MG_000374"
     CDS             30700..32139
                     /gene="purF"
                     /locus_tag="ACG8MG_000374"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFQ0374453.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            32162..33214
                     /gene="purM"
                     /locus_tag="ACG8MG_000375"
     CDS             32162..33214
                     /gene="purM"
                     /locus_tag="ACG8MG_000375"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFQ0374454.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            33211..33789
                     /gene="purN"
                     /locus_tag="ACG8MG_000376"
     CDS             33211..33789
                     /gene="purN"
                     /locus_tag="ACG8MG_000376"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFQ0374455.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            33786..35321
                     /gene="purH"
                     /locus_tag="ACG8MG_000377"
     CDS             33786..35321
                     /gene="purH"
                     /locus_tag="ACG8MG_000377"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFQ0374456.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            35337..36611
                     /gene="purD"
                     /locus_tag="ACG8MG_000378"
     CDS             35337..36611
                     /gene="purD"
                     /locus_tag="ACG8MG_000378"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFQ0374457.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            36733..37647
                     /locus_tag="ACG8MG_000379"
     CDS             36733..37647
                     /locus_tag="ACG8MG_000379"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFQ0374458.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(37780..39345)
                     /locus_tag="ACG8MG_000380"
     CDS             complement(37780..39345)
                     /locus_tag="ACG8MG_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0374459.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(39338..40789)
                     /locus_tag="ACG8MG_000381"
     CDS             complement(39338..40789)
                     /locus_tag="ACG8MG_000381"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0374460.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(40803..41819)
                     /locus_tag="ACG8MG_000382"
     CDS             complement(40803..41819)
                     /locus_tag="ACG8MG_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0374461.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            42039..43127
                     /gene="chvE"
                     /locus_tag="ACG8MG_000383"
     CDS             42039..43127
                     /gene="chvE"
                     /locus_tag="ACG8MG_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0374462.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            43181..44707
                     /gene="mmsA"
                     /locus_tag="ACG8MG_000384"
     CDS             43181..44707
                     /gene="mmsA"
                     /locus_tag="ACG8MG_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0374463.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            44709..45914
                     /gene="mmsB"
                     /locus_tag="ACG8MG_000385"
     CDS             44709..45914
                     /gene="mmsB"
                     /locus_tag="ACG8MG_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFQ0374464.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            46171..47148
                     /gene="guaC"
                     /locus_tag="ACG8MG_000386"
     CDS             46171..47148
                     /gene="guaC"
                     /locus_tag="ACG8MG_000386"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFQ0374465.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            47457..48536
                     /locus_tag="ACG8MG_000387"
     CDS             47457..48536
                     /locus_tag="ACG8MG_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0374466.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            48801..50225
                     /locus_tag="ACG8MG_000388"
     CDS             48801..50225
                     /locus_tag="ACG8MG_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0374467.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(50267..50524)
                     /locus_tag="ACG8MG_000389"
     CDS             complement(50267..50524)
                     /locus_tag="ACG8MG_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374468.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            50686..51795
                     /gene="pdhA"
                     /locus_tag="ACG8MG_000390"
     CDS             50686..51795
                     /gene="pdhA"
                     /locus_tag="ACG8MG_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFQ0374469.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            51798..52775
                     /locus_tag="ACG8MG_000391"
     CDS             51798..52775
                     /locus_tag="ACG8MG_000391"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFQ0374470.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        16599 a         9349 c        11641 g        15230 t
ORIGIN      
        1 tattagcaaa ataaaagctt tttttattgt gaataaatga acataattag gaggagaaag
       61 atgaacttat ttgacatttt tcgatcgact ttgacagata tgtcgattgt cagtgcaatc
      121 acttcaacag tttttattat attattaggg tttgtttgta gaaaaaaggg gattttttct
      181 gaatctgttg gaaaaattct atcgaaagtc gtcttaacag tggctttgcc agcactggca
      241 tttcgagctt tcatgcaaga tatcaataca gaattactga tgcaaggaat caatgtcttg
      301 atttggggat tagttattta tattattctt attctagttt caaaaccttt tttcttaagc
      361 tataaagggg ataagcaaac aacgttgcgt gtcctgacga tctttggttc tacgaccttt
      421 ttcggaacgc ctatcgtcag tgcgatttat ggtcctgtcg gcgtgatgta ttcctcaatt
      481 ttcaatattg gctaccgaat ctttttgtat tcttatggat acataaaaat gagtggcttg
      541 aagatggaag ctaaaaatat caaagcgatg ttcgtcaatc caatcattat cgctactttt
      601 gccggccttt tcatttggct gttccaaggg tatctgccac aagtgtcagt tacaggtgca
      661 gaaggagaga taagccaagt agcgtttttg cgaattgacc agactgcacc gtggctattt
      721 cagtcgatgg attatttagc tggtttaagt tcaccattgg cgtggctagc aatcggcgca
      781 acattaggtg aagtaagctt caaaaaagct gcatctgaaa aaacttcatg gtattacagt
      841 ctagtcaaag tggtatttgt acctgcattg aatatcgtga ttctagcagt tttgacggca
      901 acgaatattc taccagtcaa ttttgaagca ctggcaacaa tcgttatcat gatggctaca
      961 ccagctgcaa cagtggcagc agcctatgct atcagttttg atagggaggc acttttagcc
     1021 tccaatgcat ccttactgtc aactattttt gctgtggtac tgacacctgt atggatcgtt
     1081 gtattacaaa tgattcatgg cataggttta ttttcataag aaatggagga agaaagtatg
     1141 agaatcgcat gttacggtgt tcgtccgaac gagatagatt tttttgaaaa attgaatatt
     1201 taccattatg atctcagtct ttatgaagaa ctgttgacac atgacaatat cgtgtcagca
     1261 aaggaccatg atgctgtatt gttgagggga aattgtatag cagatgagac caatcttgca
     1321 aaaatgcagg aatatgggat tcgttatgtt tttacacgga ctgtgggagt caatcatatc
     1381 gatctacaag cggcagctga ttttggtatg acagtagccc gtgtaccatc gtattcgcca
     1441 aatgcgatag cagagttatc tttgactttt gccatgatgc ttttgagaaa tactgcgtat
     1501 acgacgatcc gaacatcttt caaagatttt cgagtggatg agcaaatgtt tagtcgtgaa
     1561 atcagaaatt gcacagtagg gatcatagga acaggcagga tagggttgac ggaagctgtt
     1621 ttgtttaagg gactaggagc caaagtgatc ggctacgata ttcatcagac agaagctgct
     1681 aaaaaagtat tgacgttcca ctcgcttgaa gagttattag ccaaaagcga tattgtcagt
     1741 attcatgtac ctcatatccc aggtgaaaac gatcaaatga tcaatgaagg atttttagct
     1801 caaatgaaaa aaggtagcat cttgattaat acagcccgtg gagagcttca agataatgaa
     1861 gctattttaa gagctttgaa aagcaatcat ttagctggtt ttgcaaccga tgtttttgca
     1921 aatgaaacag aggttttttt ccgatccttc aagccatggg agacgattcc agaccctgct
     1981 atccagcaat tagtagaaat gtaccctaga gttcttgtaa ccccgcatgt aggttcaaat
     2041 acagatgagg cattatcaaa tatgatttcg acaagtttcg agaactttcg tgaaataata
     2101 gaaactggaa aaacgaaaaa tgaggtatct cttcctaaag cccgtcaatt aagataagaa
     2161 aacatggata ttcgtcaata aaatgtttaa aaatagtcca agtaaacagt tgttacttct
     2221 gtatacttgg actattttgt atgggataat tcgatgactc aacagtaatc tgtgagcttt
     2281 ttccgcccat tctttgtcaa ttcataacag gttagcggcc ggccgatttc caaatatttg
     2341 attctttcgt tcaatatatc ttgttctact aaaaaattca aatatttctt gacggatata
     2401 cgtgacagtg agcttttttg agccagttct tttgttgaaa aaatagtatc ttcttttaga
     2461 ataaatgcac ttattttttt taaagtcagt tttgacagac ctttaggcaa atcgatgaat
     2521 gatggtattt cgtgaatggg agtggtgtgg ttgaatactt gatcgatcaa tttctgatcg
     2581 gctttttcag tcgtttggat tgtctgagag aaagcaagat acttttcgat cgccagctga
     2641 aaacgttgca aagtgaacgg tttgatcaaa taatctaagg caccgtagcg gatcacattc
     2701 tttatggttt cgatatcatc tgcagcagtg atcaaaatca ctgggatatc atttccttgc
     2761 tgtctaagcc aagataaaaa atggatacca ctttcgccag gcatgtatac atcaagcagc
     2821 agcagatctg cgtttttttg tctgaaatat ttttttcctt cttctacatt tctgaaactt
     2881 tcgattacgt tcatattagg gatgctttcc acaaaagtac gattcaattc tgcaaccatt
     2941 ggatcatctt cgattatcag gacatttgtc atcattttga tcttcctttt ttggtaattc
     3001 tagatagacg attgtaccgc catttgtttg tttggaaaat tctaaaaatc caccgtggcg
     3061 tttgacgatt gaatggatga gattcaatcc aaacccacga ttgattccct tcgtcgaaaa
     3121 acctagcgta aatatttttt ctgagatttc ttctgatatc ccgacaccgt catcgcagac
     3181 ggtcacgatg aatacttcag cttctgtgtc atagtttaaa tgaatccgga cctgtccgtt
     3241 tctttcatgt ttttgtagca cggcttcttt tgcattatca agtaagttcc ctaacacttg
     3301 caaaagtgaa tggatggagt catccatctc cattttaggg ataaaagaat cgctttctaa
     3361 cagcatttca acagattgtt ctttggcttc gtttattttg cctaataaaa aaccggcaat
     3421 tgcaggtact ttgatcagac ttgagacttg gccaatctct tttttataat tttgatttag
     3481 ctgctggata aaatgggtga cttcttcata cttcttcaaa tcaatcaaac cagaaatgac
     3541 atgaagttta ttcatgaatt catgggtttg agctctcatg gagtttatat attgtttcgt
     3601 ccccccaagc tcctccatta atttcattac ttctgatcga tttcgtaaag ttgcgactgc
     3661 tcctaaaaaa cggtcattgc taaaaagagg tgtaacgcta gaaatagttt ctatcccatt
     3721 taaatacatg gattgagtcc gaacggtcgt ttgatgctca atcgtctctt taaacaaaac
     3781 gtcatataga ttatcactga tttttttcct acttcgaaat ccaatagctg ctctgtttgg
     3841 tagaaaatgt tttttgcagg tgtattgatc aagaggatag ttttatcaga agagatggct
     3901 aagattcctt catctacttc attttcgata atcgtttttt cacttaactg ttctgctatt
     3961 tcttgtggtt caagtccaag cagtactttt ttcatttttt gggcaagaaa aacggcgcct
     4021 atgatgccta cgctaagtcc aataatcaat gaaaaaacaa tcattttttg tccatctttg
     4081 acttgattgt caatcgtgtt gtttgttaag ccaacacaaa ctgcaccaat aatctcttga
     4141 tcataataaa tagggacgaa atagcggaag ccgaacccta acattccttc tttttcagag
     4201 aaaaattctt gtcctccaat cacttttttc acatcttcat gacttgagaa aggaagaccg
     4261 atgtaacttg ggtctgcgtg agaatatcga atgccttcag ggtccatgac aacgataaaa
     4321 tctaaattcg atgcaatcat gatttcattt gttattcctt gaatcgcatg tgtcgggtca
     4381 ccagtctgca gctttttttg gatgagttta tcattggcaa cgattttagc aatgctagag
     4441 attttatctg acacactaga tcggttcatc gaaagaataa aattcctgat caatatggca
     4501 gaaacaatca aagaaacgaa tgtggagaaa caaacgacaa ggatgatcgt gttctggagc
     4561 tttaatggtt ttttcttaaa aaaaggaaac ataggctacc tcgaaagaat ttattgtgaa
     4621 aattttaaca tgaaaacaaa aatttaacaa attgtcaacg ataaacacaa ccttcctata
     4681 aaaaacacca ctgagtcggt aaaagaagct atacagaatt ttcaaacatt ggtatgatag
     4741 acgtagaaat ggaaggaggc gtgtatatga tagggggaat cgcacttgag acgatttatt
     4801 ggtatacatt gctgatttgt gcaggattgg cagtcctatt gattattttt ggagatgtat
     4861 tcgattttga tggcccgctt gatccgatgc ttattatccc ttggttaact ttcacaagct
     4921 tatttggttt tctaggtgag cgtttaacag atcaatcatc tttattttta tttattgcta
     4981 gtggaggcat atcgatagtg ttggtatttt tgcttaattt ctatatattg atgccgatga
     5041 aacatgccga atcaacgatg tcgtcttctg aaaaaactct ggaaggacaa gtagctgttg
     5101 tgatcacacc gatccctgta cgaggtatgg gagaaattca gttgaaaagt gttaccggtt
     5161 ccattagtcg tccagcttgt ttttacagcc cccaagaaca gacagctgag cggggaacga
     5221 aagtattgat cattgagatc aaggaaagaa tctgttatgt catgccttat gaaagcatga
     5281 taaaagctta aaggagcgtg tgttaaatgg aaataccagt tgtttttatc gcgatcgcaa
     5341 tcgcagcttt gattttactg atgttgctga tcgtctttgt agcgaagtac cagacagcaa
     5401 aaccggatga agcgttgatc atcagtggaa gttatttagg aagcaaaaat gtgcacattg
     5461 atgaaagcaa taacaaaatc aaaattgtgc gtggaggtgg cgcattcgtc ttgccagttt
     5521 ttcaacgttc gaatcggatc agtctattat cgagtaaatt ggacgtctcc acccctgaag
     5581 tgtatacgga acaaggcgta ccggtgatgt gtgatggtac ttcgatcatc aaaatcggtt
     5641 catctgtaga agagattgct acagcagcag aacagttttt aggcaagaca agagaagaac
     5701 tggaaaatga agcaagagaa gtattagaag gacacttgcg ctcgattctt ggttctatga
     5761 ctgtagaaga aatctatcaa aatcgcgata agttcagtca aagcgtacaa gaagtcgctt
     5821 ctgtagacct tgcaaaaatg gggttgatca tcgtttcttt cactatcaaa gaggtcagag
     5881 acaaaaacgg ttatcttgac tctttaggga aaccaagaat cgcccaagtc aaaagagatg
     5941 cagatatagc agaagcggaa gctctaaaag aaacgcgtat caaaaaagct caagcagaaa
     6001 aagaatcaca agcagctgaa ttacagcgac aaacagagat cgcagaatca ttaaaagaaa
     6061 aagaactgaa attagcaacc tacaaacaag aacaagatgt tgctaaagca aaagcagacc
     6121 aagcctacaa tttagaaagt gcccgagcac aacaacaagt aatcgaacag gaaatgcaga
     6181 ttaaaatcgt agaacgtcaa aaacaaatcg aactggaaga aaaagaaatc acacgtcgtg
     6241 aaaagcaata tgattcggaa gtgaagaaaa aagcagatgc cgatcggtac gctaaagaac
     6301 aagaagcaca agctcaaaaa gtcaaagaag tgactgaagc cgaagcagaa cgattccgtg
     6361 tggaagcact ggcagaagcg gaagccaata agacacgttt agctggacaa gcagaagccg
     6421 aagcagcact agcaaaagga aaagcagaag ccgaagcaaa acaaaagatc gccaatgcct
     6481 tcaaggaata tggcgaagca gctgtcttga gtatggttat cgatatgcta ccgcaattga
     6541 tgcgcgaagc agcacagccg ctaggaaata tcgaaaaaat ctctgttgtc gatacaggaa
     6601 gcagttcagg agaaaccagt ggagcgaacc gagtgacaaa ttatgccaca aatttgcttt
     6661 cttctactca agaaacacta aaagcgacaa caggacttga cttgaaggaa atcgtagaga
     6721 atttgtcgac acgtgggact actagccaag taaatatgta tgaagcgcca aaagaggcag
     6781 aacaaaactc gatgaaggaa gaataaagta gtacacttca ccgttcaatc aaaaatggag
     6841 gaagactttt taaagttctt ctctccattt ttttatttta ttgcaaaaga aaattagtcc
     6901 tcgctatttt tcttttgttt ttggatctta gcttctatct cttgtaaatt tgttcttagc
     6961 aatcgttctt tcttttggag ttcgaccaaa tcttctgata gttcttcctt cttttctgtt
     7021 tcaggaggac gaagaaagaa attgataccg attgcaatga atgtccctat aaaagtatct
     7081 agtacacgtt ggatagcata caaagcggat tcgccttgag ggatgctcaa cgcaatcagc
     7141 aacaacgtag ctattgcgga aatgataccg gaattattgt taataccatc tgacacgacg
     7201 atcacaataa taactagaat aggaagcaag atcaattcta ccaaaaaatc attgtgaaaa
     7261 tagcttttga caaaaaaata aatgatagct aatgtaccac ctaatgtatt gccgatgatc
     7321 ctagactttc caaaagaaac ggttgtagtc aagtcctgac gcaaagaaaa aacagcagat
     7381 aatgcagcaa tcatcgggat tcctcgttga gtcaaatgaa aaaacaagat acataaaaaa
     7441 acagctaagg cacttttgat cgtacgcata cctagtcgaa aacggccaaa ttgcatagag
     7501 attctccttt ttggtctttt tactatcata cctaaacttt tgtctgatag ctagtttttc
     7561 attattcaag tataattaaa ataaaaaagg gggaagtaaa tgcgtatagg aatgcgaaca
     7621 gtcaaaacag tgatcagtgt cttcttttcg atggtcacag cgggtagctt atcgctgttg
     7681 tattggccag ctgcagggat cattgcatta ttaagtgtag gaaataccaa gcgctctaca
     7741 ttgcgtacgg gcatgaatcg aatcgcagcc tttatattag ctacggcaac agcactgttc
     7801 agtttttttg ctgttgggta tactattttt ggcttcagtc tttttttatt cctgttcatc
     7861 ccgcttgcct ctcgcttcaa attaaccgaa gggatagtag taaattcggt tttggtcact
     7921 cattatttag tggaggaaaa catgtcttgg cagctgatcg gcaatgaagc gggattaatg
     7981 gcgattggat tagtatttgc tttgcttgca aatgtttaca tgccagatcg tacgaagcag
     8041 ctgaaggaaa accaaataca gatcgaaaaa gaatttcgaa atattttgag acaaatggca
     8101 gaatttcttt tatcagaaaa taaagaagat gtgcagatca aatgcgaaca tttactgaca
     8161 ttcattcgcg aaagccaaga agatgccaga gaacatcaag aaaactactg gctcagacag
     8221 ccgctatact acgaaactta cttctctatg agaagagcac aggccaatgt gattaaagac
     8281 atgctggaaa atctagagag aatccagcaa ccagcttatt atggaaaaca tatctatggt
     8341 ttattgattt atactgcaga aacattttca gagtcaaatg atggcagaca aattttaaca
     8401 cgtattgaag aagtttatgt attgtatcgg caaatgcctc tgccgactag tcgccctgaa
     8461 ttcgaggatc gagcagaatt gttccagttt ttgcagtctt ttaaaagttt tattgaaatc
     8521 aaggcagaat tctcccaaca aaaaatccaa aaatagaggt tgtgaattca gctaatattc
     8581 aggtcaaaaa gctgatttca caacctcttc tttaaacggt gttctcctgt caactcctcg
     8641 gtcacaagtc acaatattca acaaacatga gaagctgttt tttgaaattg ctccttgtga
     8701 ctcggagctg aacggtaggc tcacaacctc tttcttaaac ggtgttcaaa agctttcctc
     8761 tgcggtaata agtccagcca aacgaaaaat aggaggagct atttttgttt ggctgttctt
     8821 attacttgat gcagaacagc tttttcacaa ccttttaaaa ttacatattt ttccatagaa
     8881 cttgcatttg atccaagatt tcgatgacat cttttgtcaa gaagagagag cgattgggca
     8941 agtcaccttc aatcatgtgt accatattct ttatttcgta attcattgct tcagatgtat
     9001 tcccgctttc gatgaattcc tttgtaccat ctgtaaataa gatctccgca cgatcagcac
     9061 gcgggtaatc agcaatcgtc acataaccgt tctcaaaggc aacgataccg acttttggca
     9121 tttttgcctg aaaagtcaag ctgacagcag caagttcatt ttctttgtta cgcaagatag
     9181 tgacggattg ttcgtccacg cctgtttcaa aaggaaccat tgtagaggca actacttcag
     9241 gttgagagct caaaaagaaa cgagcgaaag atacagcata cgtaccgata tccaacagag
     9301 ctcctccagc taattctgga ttgaagaacc ggtttttagg gtctggttct ttgtaactgc
     9361 cgaaaggtgc ttggatcatt tttaaagccc ccagttttcc tgtatccatt attgagcgta
     9421 actgctgata aagtggcatg ttgaagattg tcatcgcttc tgctaagatc aaattctttt
     9481 cttctgctaa gcgcatagcg tcagccagtt cttttttatt catcgtgatg gctttttcac
     9541 atagaacgtg tttgcctgct tccaatgctg ccaagatatg gtcgatatgc tgtctatttg
     9601 gtacagcaat gtacacgatg tcgatttcag catcgtctaa catttcttga tagcttccgt
     9661 aagcttttgg aatattgtaa cggtgagcga agttttccgc tttttctgaa gttctggaag
     9721 caactgctgt tagttcgctc gtctcttgat tgaagctttc ggcaaactca tgtgcgatgg
     9781 taccaagacc aacgattccc cattgatatt ttttcatctg aaaacgctcc ttcatagttg
     9841 gattgagttt cttgtctgtc tattattata acaaaaatca cgaagaataa agaggagagg
     9901 acttgaaaca ttaaaaaaaa tgaaagcagc gatttaagag gtgctttttg atcaaaactt
     9961 aagtagaata gttaaaagga ggggataaac gtgcatcaag tatatattga tcttattatg
    10021 agtgtattat tagaacagtt tgaaactgaa aaaaattttt tagatgaata cttgctgatc
    10081 agtccagaac aatggcaaaa atggaaagat ggcgagaaaa atctgccatc tgaagcgatg
    10141 cagaaagtaa aaaatctttt cactgattat gaatggatgc taacgcaaaa gattttgcgt
    10201 caaacgatta tttttcccga aaaacgaaac gttgccgttg cagagtacaa gcaaatcaaa
    10261 acacgcatcg cgcaaaaatg gcttagcagc aaagcagcaa aagtcgagct gatccctttg
    10321 aaaaataatc agcaggctgg tagatacctt gacttgaaag taagtattca atatgatgaa
    10381 tggggctatg atgatatttt gaattttcgt ctgccagcag atgttcaaaa acagatcgaa
    10441 ggagaaaaaa tcgaacttct cgattgggtg aatgaaaatc tggaagaaac ttacgttcat
    10501 gaggcgaatg gggaacgtga agaacaacga aaggattttt caagtgaata aatttttagg
    10561 atttggagca gttgtgatta tgatcgcagc aatgggctat gttattttta gcgatgatga
    10621 ttcgcatgtt caagccggca atcaagcagt cgaacgaaca agcaccaaaa caactgctag
    10681 taaagaaaag aacagtcaaa caaccagcag caaggtaaag gacaaagaaa caaaagatcg
    10741 tacgtccaaa caattgccag atgttaaaac agacgattgg aagctagttt tggttggtcc
    10801 agatcacaaa ttagaaaaag aaattgatga gtctactcaa ttggcatcat taagtaacgg
    10861 atacttgatc gacaaacgca ttgctccaga atatgaagca tttgccaagg ctgctgaagc
    10921 agctggattt ccattagtga tggtctcagc ttaccggtct gtatcatccc aacaacaagt
    10981 tttcgaacaa aatgtccaag atgtgatgag tcgtcaaggt gtatcagaag aggaggcgac
    11041 aaagattact aaagaaacga ttacggagcc aggatacagc gaacaccaca caggtctggc
    11101 agtcgatgtc gtcgatcaaa actggtacaa cagttatcct tctactgtat tggatgcaag
    11161 ttacggagat cagcctggag ctaaatggat cgcagataat gctgcgcagt atggatttat
    11221 cgttcgttat ccaaaaggac acgaagatat aactaagatc acatatgaac cttggcactt
    11281 acgttatgtc ggtaaagaaa acgctgagta tatcaccaaa caccaattga cgctagaaga
    11341 atatctgaat gaattaaatg aaaagtagga attgatcatg gcgaaaaaaa agtggaaaaa
    11401 gcaaaagcgc aagaatatcc gttggccagc agtggttgcc ggtgtagcag tgatactctt
    11461 agcctttgtt ttttccttga agaatctgtc ttctcctttt actgataatc agcaagaaaa
    11521 tgaagcgcaa tcccatcagc aatttattga acggcttgtt ccgcatgccc aagaactaca
    11581 agatggttat ggagtgcttc ctagtatcat tttaggacaa gctatattag aatcaaattg
    11641 gggaaaaagt accttggcta gcaaatacaa taatcttttc gggatcaagg cttatgggga
    11701 tcagaagaaa gtaagcctgg aaacaaagga atttgttaac gaagaatgga taactatcca
    11761 aggagacttc aaagtgtatg attcatggga acagtccatg gatgaccata cacaactgtt
    11821 cgtccaagga gtagattgga atcctgcgct gtatgaaaaa gtgattacgg ccaccaacta
    11881 tcaggaagcc gcacaagcat tgcaagatgc aggttatgcg acagaccccg gttatgcgca
    11941 aaaaatcatc caggtcatcg agacgtatca attgaatcag tatgaccgga gatagcaaag
    12001 gagaagtggc tgatggcaga acaatttacg ccagaaatca tgacggaatt gagaaaagat
    12061 atcgtaagag taccggaaat cattttagaa gcaagcggga tcaagatatt tggaaaattg
    12121 atccgttcga tcatctttac tacggatatt gcgatcatac gtaatacaaa tgcgaatgca
    12181 gtaattgctg tgtatccttt tacacctcat ccagcgatta ctaaaagcat tattgaggca
    12241 gctgacattc cagttttctc cggtgtaggt ggtggtttga cacaaggtgc tcgttcttcc
    12301 tacatgagca tgttcgcaga agcgcaaggt tcgttagggg tcgttttgaa cggtcctacg
    12361 cctttagaga cagtaaaaga agtttgtcga gtagttgata ttccagttat cagtacggta
    12421 acgtcgaaat atacagaaat agatgaaaag ttagcagctg gtatttcgat tatcaatatc
    12481 agtgcaggta aagaaactgc agaagcagta cgccattttc gtaaaaaata tcctgaactt
    12541 cctatcattg cgacaggtgg tccaacggaa gaaagtattg ctgagaccat tcaagcagga
    12601 gcaaatgcca tcacatatac gccaccaagt agcggagagc tttttagtcg taaaatggaa
    12661 aaatatcggg aaactgagaa aaagaatcat gaataacagg tgagaatcaa aagacgagga
    12721 agagcagata gtggctcttc ctcgtctttt gtactccagt actatttgtt cagaattcaa
    12781 aaaaaagaga aatttttttg tttttttacc tgtaatgtac ataagaagag ttaaaacctt
    12841 ttgttattat tagcattcta acaaaaaaat gtcttgattt attgaagcgc tttctataga
    12901 atgaaaaacg aataaaaact aaaaaaggca cccgacaatt tagtagcagc taaattgtcg
    12961 gggcttgaaa agtcagcggg aactgaccaa tcaagttgcc atagtcaatg cttgtgcatc
    13021 gacgtgtata ttgtacctaa tttcatacag aattactagt tatttttctg ttttttaggg
    13081 aagagcgcac acgaagaagc atgcttattt agctatgtca aggatattgg gcagttgtct
    13141 gatatccttt tttctttgcg taattatcct cccatcattg aagcaagaag tgtttttagc
    13201 gatgaaagaa gaatcagcac tatctaattt ttcttgaaag tattgagttg ttcatcctag
    13261 gcagaacgat aaggcaagtc ttggttttta ttccccttga aaaagatcgt ttaataaata
    13321 tgttctgtat aaaaatacag tatattgaaa aataacaatg gaagtagagg tgcagagaat
    13381 gaagaaaaga atgagaatca tcttagcttt aagtgttatg ttattcttct tgacggcttg
    13441 tgggcaagaa gatgacaaaa aaatcacgct gaacatggct tggtggggtt cgcaagtccg
    13501 acacgatgca acagtaaaag taatcgagct ttatgaagaa aaaaatcccc atgtgacgat
    13561 cgattatgaa ttttatgatt atgaaggata ttacacaaaa ttaggtacac tagcagcatc
    13621 agatgatatc tgggacattt tccaactgaa caatcaattt cctcaataca tcaatcagat
    13681 tgaaccatta gatgaatatg tggagcaagg gataattgat atttctaatg cagatgaatc
    13741 ctatttagca acgacgactt acgacgggca attagttgga ctgtctaacg gggtgaattc
    13801 gtttgcgatt gcttacaata aaaatatatt tgaacaatta aatatccctg aaccagcttc
    13861 aaattggaca tgggatgaat ttgaacaaat cagctatcaa atcactgaag aattaggaat
    13921 ctatgctatt tcaagattcg aagattttat agctggctgt attattcaaa taccacaagt
    13981 tgagaaagga ttgaattttt ataatcggga agatctatct aacagcttgg gatttgaaaa
    14041 tccagactat ctaacagatt ttttccagct gcgtaaaaat ctggtagaca aaggggccca
    14101 tccagaacca ggtggtggag ctgcaagtac gacggatctg tcttttcagg cggttaggga
    14161 tagagaagcg gcaatggtgt ttctatcaag taaccagttg acagaaatat tgtcaggggc
    14221 tcctgaagat atggagattc agttgattaa tccacctcgc agaaaagcag atggagaatc
    14281 tggtatccct ttacgttctt cgcaaatgct gagtatggca aaaagctcag cccataaaga
    14341 agaagctgct aagtttattg atttctttca aaacagtgaa gaagccaatg aaattttaaa
    14401 aggagaacgt ggtgtaccaa tcatgtcgaa tattcgagaa cagctcgctc gtgcagatga
    14461 tgcaactttg caagcgacat ttgattattt agaaatgatt tccgatatgg acaacggcga
    14521 agtgaatgtc tttgatagtg ctttgaatcc ggaaattcaa gaccaataca atcttttagt
    14581 tgaaaaagtc atttatgaag aagtaacagc agaagaagca gcagagagtc tttatgagtt
    14641 tgcgtcaaac attctaggat agcagcagga ggaaggcatg aaaaaagaaa aactaaaacg
    14701 gttcttggat caagacaatg tggtcggtta cgtatttgct tctccattca tcctaggatt
    14761 tctattgata acagtgatac cgatgatctt gtcactttat tattcgttca ccgattatcg
    14821 tttgggaaat ccagtctcat ggatcggctt ggataattat ataaagatgc tgagtgatgc
    14881 ccgttttttg aactccatcc gagtcacttt tctctatgta tttgcatctg tacctgtgaa
    14941 actgatcttt ggtctattga ttgcttatct tttaacgagg aatatcaaag gaacgacttt
    15001 ctttcgttct ttgtattatg tgcctagttt gatcggtggc agtatttcta tcgctctagt
    15061 ttggcgggag ttgttttcta aaaacgggtt agtgaatatt ttcctggaac gattaggcgc
    15121 acctgcgctt tcctggttta gcgatcaaaa actggtcatg atccctcttg ttttgatgtc
    15181 tgcttggcaa ttcggttcgg caatgatcat ctttgctgca ggtatcaaag agatcccagg
    15241 ctcgtattat gaagcggcag agatcgatgg agcgaacaaa tggcagacgt tttacaagat
    15301 cacgttacct cttctttcgc cgatcatttt gtataacttg gttatgcaga caatcgcggc
    15361 gtttatgact tttacgcagg catttatcat tacccaaggt ggtccgaatg atgccacgaa
    15421 cttttatgct ctttatgtgt ataaccaagc tttttctttt aattctatgg ggtatgctag
    15481 tgctatttcg tggatcatgc tgttgattat gggagctgtc acctttctga ttttcaaatc
    15541 atcaaaaaaa tgggtcgttg tgtgaatgag aggaggaaga aatgaataat acagtaacga
    15601 aatggtttca atacggtttt attttgctat taggtctagt catgatttat ccggtcttat
    15661 ggatgatcgc aggttctttt aaaacggatc aagagattat gagtggttcg ctgaatttga
    15721 ttccagagac ttttcaatgg gaaaattacg caaaaggatg ggcggggttt gctggaattt
    15781 cattttttac tttctttagg aattcgtttg tcatctctgc agtttcgacg atagggacag
    15841 ttctgagctc gacttgtatt gcttacgcat tatcgagaat cgatttcaaa ggaaaaagat
    15901 tctggttcgt cgtcatgtta gtaacgatga tgattcctgc acaagtgatt ttgattccgc
    15961 agtttattat ttacaatcgg ctgaatttgg ttgggacgta tgtgccgctg attttaccac
    16021 atttttttgg acaagcattt ttcatttacc agatcatgca attcatggtc agcatcccca
    16081 aagagttaga tgaatcagcg attattgacg gatgcagcaa gtatagtgtc tttaccaaaa
    16141 ttatttttcc tttgctgaaa ccttcgatca tcacgacgat catcatccag ttttattgga
    16201 aatgggacga cttcatggga ccattgattt atttaaacaa gccgagatct tacacggtat
    16261 cgattgccat caaattattt gcagacgctg gatctagtac gacttacgcg agtatgtttg
    16321 cgatgtctac gttgtcgctg ctaccggttt ttctgatttt cttattcttc aataaatatc
    16381 ttgtccaggg gatcagtaca agcggtctga agggctgaat atgaaaaagc caatagaaga
    16441 gatgtttctt tacaagctca actactatgt ctgtcggatt tttttaacca gtcttatcag
    16501 taatgctctt ttcgctttag ttctaggatg tttgttcact ttaggattag tgctgtctgc
    16561 tcgtgttgtc tatctgattt attttttgat gatgtatcct tcttattatc tatctgtcca
    16621 ttacttggca gaaaaaaagg tatgttggag gaaaggaaaa atcaaacgga tgtgtttcat
    16681 cattacgttg tttcagctgt ttgtcattat ttttgcagtt gattggtatt tattagtggg
    16741 aaagattgga gcttggtatc tgattccttt tctattgtgt ctgttgcttt ttgtatccca
    16801 tagttttttt agtacgctaa ttgatatctg caaagaagat aaagcagata gtcttgttta
    16861 tttgatcaaa aggaatatct ggctgttgtc aaaaaacgtc aaggggtttc tgtggtattt
    16921 atgccttttg tatggactgt tctggtgtat acagcatgat tttttactca ctgcattact
    16981 gtatccaggg ctgccatttg tcacagtatt ttgcttaaaa gaaaaggaga atgactttaa
    17041 atgaagaaaa aatatgtaca agtcggactt ggtgggagag cgcgtttttt ttatgaggca
    17101 attgcctcta cgtatcatca aacttgtgaa ctagtcggtt tttgtgatgt atctcaaaca
    17161 aggatggatt atgcaaatcg gatacttgtg aacaagtatg gacagaaaaa agtaaagaca
    17221 tgtatgccat cacaatttga gcaactactg gatgacgtac ggccggctga agtcattatt
    17281 acgtcagtag atcgaacgca tcatgattat atcattcgtg caatgaaaaa aggatatgat
    17341 gttatttgcg aaaaacctct gactatcgat gaggaaaaag ctcaagaaat aataacggtc
    17401 caaaaacaga cgggaagaag cctgcgagtc acttttaatt atcgttatgc accgtatcac
    17461 acgaagataa aggaactgat agaaagtgat gtgattggtg atgtgttttc tatccacttc
    17521 gaatggctgt taaatacgca acacggggca gattatttca gaagatggca tcgaaataag
    17581 cacaatagcg gcgggcttct tgttcataaa tctacccatc attttgatct agtgaatttt
    17641 tggttgaaca gccgtcccca aaaggtattt gcattcggtg gattaaactt ttatgggatg
    17701 cataatgcgg agaaacgcgg agtagaaata ttttacagtc gatgctatgg aagcgaacta
    17761 gctaaagctg atccatttgc tatagacctg tcagccaatg aggaattgcg agaaatgtat
    17821 ttagaagcag aaaaagacag tggatatatt cgtgaccgca gtgtattttc cgatgatatc
    17881 tctattgaag atacactagg cgtaacggtc aaatatgaaa ctggtgcgat tttgacgtat
    17941 tcgctaaacg cgtatatgcc gtgggaagga ttcaatgttt ctttcaatgg cagcaaagga
    18001 cggatcgaaa tgaaagtgat cgaaaagtct tacatcaatg caggtggaga taaaaaggat
    18061 gaaggagcag ccgttcttca gtccatccat gtctatccga tgttcggtga gccatatgag
    18121 gtaatcgtcg aacaagaagc gggcggacat ggcggaggag atccgcgttt attagatgat
    18181 ctatttggtg aagaaaaaga agaagatccg tggaatcggg cagcaactca cgtagatggt
    18241 attttgtcga ttttgacagg aatcgcagca aatcattcta ttgcttcagg aaaagcagta
    18301 gtgattgacg agcttgtcag cttaaaagaa taagaataga tttacggaaa ttacttcccg
    18361 tagtttataa aatcttgcct tattgccgat aggctgtctt ttgaacacta tttattcgga
    18421 aaaaggggct atgacatgct ttttgtcatg ccttcttttt atgtgggctg cttttttaga
    18481 taggagaaaa taaaaataac tatttaaacc cgaacaaaaa ggaataatgc attaaaaatc
    18541 attagatttt acaaaaaaat aagtaaacca cgttgtcagt ttcagaaagt agtgttagac
    18601 tgttttcaat atttgaaaca acatttttta ttcaaatatg gcaaactgcc ttcacgtgca
    18661 aaacagttgc aagaaaatga atttacacta ttattagttt tacataccta cttatatatc
    18721 gtcataatat ggatgacagt ttctagccag taccgtaaat gctggactat aagtaaaaga
    18781 ttggctattt cacccttctt ttcttatgat aaagtggagg gtgtttttct ttaacagaaa
    18841 catagtaggt ttttgcacaa aagcggttat ttcggtatta ataaatgata aaggagcaaa
    18901 taaagtgaaa gaattagttg aacgtattca aaaagacgga cgtatattag gcgagggcgt
    18961 attaaaagtc gacagcttcg tcactcacca agtagaccct gtattgatgg aacaaatggg
    19021 aaaacggttt gcagaagtat ttaaagaaca aggaatcacg aaagttgtga ccattgaagc
    19081 ttctgggatc gcaccagctt tatttgctgc aagagaatta gatgttccaa tgatcttcgc
    19141 tagaaaagca aaaagtttga cgatggatga agaattattg acagcttctg tttattcttt
    19201 tacgaaacaa gtgacaagta cgatttctat ttctcgtaaa tttttatctt cagaagacaa
    19261 agtattgatt atcgacgatt tcttggcaaa cggacaagcg gcaaaaggat tgatcgaatt
    19321 atgccagcaa gctggagcgc aagtagaagg aatcgggatc gtcatcgaaa agtctttcca
    19381 agatggacgt cagttacttg aagaaatggg attgagagtc gtttctcttg cgcgtattgc
    19441 ttccttgacg aatggacaag ttgagtttct tgaggaggac gcataatcgt ggaaaaagaa
    19501 acacaaaatg gaaaagctgc ggttttaggg ctgcagcatc tgctagcgat gtatgcagga
    19561 gcagtagcgg tgcctctttt gatcggtaca ggactaggct ttaatcaaga acagatgact
    19621 tatttgattt caattgatat ctttatgtgt ggattagcga cattattgca attgacagtt
    19681 aaccgatttt tcggaattgg cttgcctgtc gttttaggat gtgcgatcca agcagtagct
    19741 ccactgatct tgatcggaac caatgaagga gtaggagcta tctacggatc tattatcgct
    19801 tctgggatct ttgttgtgtt agtttctggt gtcttttcta aaattaaaaa actattccct
    19861 cctatagtaa caggaacggt tatcaccgtt attggtttga ctttgattcc tgtagcaatc
    19921 gagaaaatgg gtggaggtaa tgcaacagca gctgattttg gtgacaaaac aaatctgcta
    19981 cttgcatttg tgacgattct cctaattgta ggcgtgcaaa tgctggcaaa agggttcatt
    20041 cgttcaatcg cggtattgat tggtttagtc ggaggaagca tcttagctgc tttcatggga
    20101 atgattgatc tgagcgcgat tggagatgcg ccgttgttcc atgttccaca acccttttat
    20161 tttggtaaac caactttcga cgtatggtcg attttactaa tgatcatcat ttccatggtc
    20221 agcatggtag aatcaacagg tgtatatttt gcacttggtg acattactgg gaaaaatgtt
    20281 ggagaagaag aattgaaaaa gggatatcgt gcagaaggac ttgccgttat cttaggcggt
    20341 attttcaata cattccctta tactggcttt tctcaaaatg tcggattggt tcaactttcg
    20401 ggaatcaaaa caagaaaacc catctatttc tctgcattct tcttgattgt tttaggatta
    20461 ttccctaaaa tcggtgcgtt agcacaaatc atccctgagc cggtacttgg tggaggaatg
    20521 ttagtcatgt tcgggatggt ggcagtccaa ggaatccgta tgctgttgga agttgatttc
    20581 agcaatgata aaaatctatt gattgttgct gtttctgttg gatttgggct gggtttcaat
    20641 gttacgccga cattattcag tcaaatgccg caaacattgc aaatgttcac tggaaatggc
    20701 atcgtaatga gtagcattac ggctatcata ttgaatttga tctttaatgg attgaaaaaa
    20761 gatgaagaaa tattggaaga agccttcaag aagtaataga gcaaaagaaa ggttgtgaaa
    20821 tcagcgtttt gacctgattg ttagagaaac aaacggaaaa atagttcctc gtattttgcc
    20881 gtttgtttga gctaataccg agggtcagct gattgaacac cgttgaagga agaggtcgtg
    20941 agcctgccgt tcatctccga gtcacaagga acaatttcaa aaaaaagctt ctcatgtttg
    21001 ttgaatattg ttccttgtga ccgaggagtt ggcaggagaa caccgtttat ccataaaaag
    21061 gaatgtgacc taacttttgt cacattcctt ttttttatct ttaataaaca cgaacaataa
    21121 aatgtttttc tttgttaaca ttcgaaattt ctttgacatc aaaaaaagtg attgttaaaa
    21181 tatgaatgta gaaaaactgg gaggaaaaac aatgacagca aaaatctctg tgattatggg
    21241 gagtacttca gattgggaaa caatgaaaga agcatgtcaa atattggatg aattcggtgt
    21301 gtcttatgaa aaaaaggtcg tttctgctca tcgaacccca gatttgatgt ttgaatatgc
    21361 acaaaacgct cgaaaaaaag gaatcaaaat cattattgca ggagcagggg gagccgcgca
    21421 tctgccgggt atggtcgcat caaaaacaac tttgccggtt atcggtgtac cggtccaatc
    21481 acgaacatta aatggtttgg attcgcttct ttcgattgtc caaatgcctg gaggagtacc
    21541 tgtagctact acagcaatcg gaaaagcggg agcaattaat gctggactac ttgcgataca
    21601 gatgctttcc atgtatgatt tagaattgga ggctaaattg gctgaaagac gttcaatgtt
    21661 ggctaaaact gttatagaaa gtagtgatca acttggttaa acctttgttg cctggttcaa
    21721 cgatcgggat cgtcggtggc ggtcaattag gtcgtatgat ggcgattagt gcaaaagaaa
    21781 tgggctttaa agtaggtgtg ctggatcctg tgagcgattg tccagccgca caagttgcag
    21841 attggcacat tgaaagtgtc tatgatgaca cctttgcgct gaaagagctt gccagaagaa
    21901 cagatgtcat tacctatgaa ttcgaaaacg tcagtgtaga ggcgttaaat gcgattttac
    21961 ccatgtcatt tattcctcaa gggacagact tgcttgcgat tacgcaagac cgcctgttag
    22021 aaaaatcttt tttggaaacg aataacatcg tgattgctcc ttatgcaacg atcgtcagtc
    22081 cgacggatat ccaagatgcg attgacggga tcggctatcc ttgtgttttg aaaaccactc
    22141 gaggcggcta tgatggcaag gggcagtatg ttttgaaaag tcgggcggat ctagctcctg
    22201 ccatggacct tttaagagaa ggaacctgtg ttttagaagc atggatcccg tttgaaaaag
    22261 agatttcgat catggtggca ggaaatggtc aaggagattt cacgactttt ccggtcgtgg
    22321 aaaacatcca ccacaacaat attcttcatg aaacgattgc tccagccgcg attgatcagg
    22381 atgtgatcga ggaggctgag cgtattgccc gtgtgattgc agaagcagtg agcctttcag
    22441 gtgttttagg ggtagaaatg tttttaacaa aaacaggcgg gctatatgtg aatgaattag
    22501 ctcctcgtcc tcacaattct ggacattatt ctattgaagc ttgttctatg agtcagttcg
    22561 atgcccatat tcgaggtatt tgtggttggc cattaggaga aaaagtacgt ttgataagca
    22621 atgcagtaat ggtcaatgtt ttgggagaac aactgctgga aagctatcgc ttgattgaga
    22681 ataagccaga ctggcaattc catttctatg gaaaaaaaca agcaaaaaaa ggtcgcaaga
    22741 tgggacatat cactatcttg acagaagaca tccagcaaac gctggaagaa atcaaacaaa
    22801 cgaatatttg ggattagagg agaaaaaaca tgttagaacg ttatacacgc cctgaaatgg
    22861 ggaatatctg gacggaagag aacaaatacc gtgcgtggct cgaagtcgag attttagcag
    22921 atgaagcttg ggcagaactt ggtgaaatcc cgcaagaaga tgtgaaaaaa attcgtgagc
    22981 atgcatcatt tgatatcgat cggatcttgg aaatcgaaaa attaacacgc catgatgtcg
    23041 ttgcgtttac acgagcggtt tctgaaacat tgggagaaga aagaaaatgg gtccattatg
    23101 gtctaacaag tacggatgtc gtagatactg cttatggcta tctcatcaag caagcaaatg
    23161 atattttacg ggaagacttg aaacgtttta cggaaatcat tggcgaaaaa gcaaaagaac
    23221 acaaatatac agtgatgatg ggtcggacac acggtgtcca tgctgaacca actacttttg
    23281 gattgaaact tgccacatgg tattcagaaa tgaaacggaa catcgaacga tttgaacatg
    23341 cggcaaaagg agtagaagca ggaaaaatct caggagcagt gggaacgttc gcaaatatcc
    23401 cgccatttat tgaagagtat gtatgtaaac aattggggat ccgtccacaa gaaatctcta
    23461 ctcaagttct ccctcgggat ctacatgctg aatatttctc agcgatggcg cttattgcta
    23521 caagtattga acgttttgca acagagatac gcggattgca aaaatccgaa acacgtgaag
    23581 tagaagaatt ttttgcgaaa ggtcaaaaag gttcttctgc tatgccgcac aaaaggaatc
    23641 cgatcggttc agaaaatatg gctggtctag ccagagtggt gcgcggacac atggtcactg
    23701 cctatgaaaa tgtcacatta tggcatgaaa gagatatttc tcattcttca gcagaacgaa
    23761 tcatcttgcc tgataccacg attttattga attacatgtt aaatcgtttt ggcaatatcg
    23821 ttaaaaatct aacggtcttt cctgaaaaca tgaagcggaa tatgtctgca acatttggtt
    23881 tgatttatag ccagcgcgtt atgctgaaat tgatcgataa gggaatgaca agagaagaag
    23941 cttatgatct tgttcagcca aaaactgcgt acgcttggga caaccaagtg cagttccgcc
    24001 cgctgttaga agaagatcca accatcacat cggtattatc taaagaagaa atagacgatg
    24061 cctttgatta tcatcatcat ttgaaaaacg ttgacgtgat attcaaacga gtaggtttgg
    24121 aataaagaag agtgagttgc aaaattcaat taaaatagaa tagcaaaaat aatcgagtag
    24181 aggggaaagc caactgacct tccgcccctc tcacaccacc gtacgtacgg acccgtatac
    24241 ggcggttcaa taagttgagt gtacgaactg gtacttcaag gagatgtcct catatccttg
    24301 gcgggccagt tctttgtctg ttaatgatcg atgaagaata tgacttttgg agactgacca
    24361 aatacctttt cttgtatttg accattcata cgctttcgct ttatcgatcc ctaaacgaat
    24421 cagatttttt cttttggttc ttggattctt ccattgtttc caaatatatt gtctgattct
    24481 tcgcttcaac caaccgttta actccctcat gaattctttc atctcaccaa ttccatagta
    24541 gttgatccaa ccagtcatta gttgcttgat ttcttttaaa ataagttgga tacttcttcc
    24601 acggtttctt ttagtgagtt gtttgagctt gcttttcaca cgctgttttg cttttgcgtg
    24661 agggcgaatt ttaatacctt tctttgttgg cagaatgcag aatccaagaa actttcgttt
    24721 caatggactt cctaccgcac tcttttctcg atttaccgtc aactttagtt gttcttcgag
    24781 aaattgaatg gatttcttca tcacacgctg tcctgctcgt ttgcttctaa catagatatt
    24841 gcagtcatcg gcgtatcgaa caaatttatg tcctagattt tctagcactt gatcaaattc
    24901 gttcaggtag atgttactta acaggggaga gagattgcct ccttggggcg ttccttcttc
    24961 agttgttgtg atgagtccat tttccattac accactcatt aagtattgac ggatcagttt
    25021 taggacttgt ttgtccttta ctttccgctc aatgaaatac atcagcttat cgtgatttac
    25081 ggtgtcaaag taggctttca tatcaatgtc caccacatat cggtaacctt gttcataata
    25141 agattttgct ttcttcactg cgtcatgtgc gctgcgattt gggcgaaacc cataactatt
    25201 atctgagaat tctggttcaa atagcggttg taacacttga ttgattgcct gttgaagcat
    25261 acgatcaata accgtgggaa ttcctagttt tcttacaccg ccgtttggtt tcggtatttc
    25321 tactcgcaaa acaggctgag gtttatagtt cccttggcgt aaattggata gtaattcctc
    25381 gcggtgctct ttcaagtaag gaagcagttg atcgacggtc atcccatctg caccagccgc
    25441 ccctttattt ctgactactt gaagatacgc ttgattgaga ttattccgat ctagaattct
    25501 cccaaacaga ttttctacac catcttgttt cctctgttca ccgcaagaag tgctgcgcac
    25561 ttcatcatac ccttcgtgtt ccacactagc tctttgatga tagccaagtt ttcttgtttt
    25621 ctgcggtcgt cgcatcttct cacacctcct aaattattca agattcttat tgttcagtcc
    25681 ttcgtaacgg tcagtgtcgt tactactatg acctcggctg acttctcatg attcaccatc
    25741 atgtcgccat gatcgttgtg ctggtttgtt ttcattcaac gcccacaaca ccctcatgag
    25801 atctcccccg gtaagagtga aaactttcct cccatgtaac tgctagattt actgtatagg
    25861 tttcgggcag tattggactt tgtcttgtga tgaagactta tccgacctaa ttcagccttg
    25921 tatctagttt ctgttcgtca gttcaggagt ttgcgtccga cttccttcag ccacaacctc
    25981 acgattgccg ccttgtcttt cgctaacagt tcctactgcc aagcctgtag tggactttca
    26041 ccaccaagtt ttcacccatg cagggcgcac accaaaaaag aagcagatcc gttagcggtt
    26101 ctgcttcttt tagtttatta tattttctta tcaataatag gaacctgatt ttaaaagcgg
    26161 ctacctcttt accgtgcctc tttacataca aaaaatagag gaattaagcg taaatggctt
    26221 ggctattata actaaaatgc tggttatgga gatgttgcag caagttttcg agaaaagcgt
    26281 attccatctc ttgtccattg aataagatct ggatacgata atgatttttg aaacgcttct
    26341 gttcatgaat ctgactgatt gaaagttcgc ctttgtgttc tgtctgataa agaaaagtcc
    26401 ttttacggca ttcagcacag cgcttggttg ctcggcggat ttttcggaat gtataaaaac
    26461 tagtatcttt attgcataaa taaacgtctt tgatcgacag caaatcatta tatagacagg
    26521 aacgttcttc agctgttaat tctgataaaa aaagctgacc ttgttttatt gcatcttcca
    26581 tcggcaagtg cctcctcgaa cccattatcc aaatttttag ttaattctaa tataccatca
    26641 ttaagtataa atgtaaacgc ttgttgatgg tataagattt tcgtttctga taaaacacga
    26701 acattaaaag aatctaatgc cttaaatgta catatttttg ttgatttata aaaagatgat
    26761 tgctaaaatt taaagtgaaa aaacgaacaa aaaacgatgt atttgctttt aatgtaaata
    26821 aaaaggagag acgtacatgg agaagaaaga acttttgtat gaaggaaaag ccaaaaaaat
    26881 atatcttact tctgatgaga acttgatttg gatagaatat ttagatcaag caactgcgtt
    26941 gaatggcgcg aggaaagacc agataagtgg aaaaggagaa ttgaataatc aaatcacaag
    27001 tgcaattttc cgttatctga ctcaaagagg aataaaaaat cattttattg aacaattgtc
    27061 taaaaccgaa caattagcaa aaaaagtaca aattatccct ctggaagtgg ttatccgaaa
    27121 tattgcagca ggaagtttct caaaacgtct aggtgttccc gaaggtaaag aattcatcaa
    27181 accaatcatt gaattctact ataaggatga ccagttggat gatccattta tcaatgaaga
    27241 gcatatctat ttcttacaac ttgcaaagcc ggaagaaatc agtgagctga aagcccaagc
    27301 gttggctatt aatgaaattt tgaccgaatt atttgctaag gtcggtttgg ttttagtaga
    27361 tttcaagcta gaatttggcc ggtcgaatgg acaaattttg cttgcggatg aagtaacacc
    27421 ggatacttgc cgattatggg atgaacgtac gaatgaacat atggacaaag acgtttaccg
    27481 cagagatcta ggtgatttga tcccagtcta tcaagaagta tataaccgtc tatcggaact
    27541 aactgaagaa aaggagcgag tatgatgtat ttcgtaaaag tatatgtaac ctataaagat
    27601 tcagtgttag atcctcaagg ggaggcagta aaaaaagcag tccatcgtat gggatatgaa
    27661 acaatcgaag atatccgaat aggaaaatac tttgagatac aacttgctga tgcagaagaa
    27721 ccaatcgata cagtgattga aacgatttgt gacaagctgc ttgcaaatgt aaatatggaa
    27781 acataccgat atgaaatcaa aaaaatggag gaagtataac atgaaatttg cggtcatcgt
    27841 ttttccaggt tcaaattgtg acgcagatat gttatgggca gttcgcgaga tcatgggcgc
    27901 tgatgccgaa tttgtacggc atgatgagtc ctctttagaa ggatttgatg gggttttact
    27961 gccgggagga ttctcctatg gtgactattt gagatgcggt gcgattgctc ggttttctac
    28021 aatcatggaa gaagtgattc gttttgctga agaagggaaa ccagtattcg gtacatgcaa
    28081 tggattccag atattgacag aagcaggact tttgccaggt gtactgagac gaaatgagtc
    28141 tttgcatttt atttgcaaaa ctgtaccatt gaaagtcaac cccaagacgg cctttacttc
    28201 agagtatgaa gccgacgaaa taatctatct tccagttgct catggggaag gaaattattt
    28261 ctgtgacgaa gaaacacttc aagaactaaa aaaaaataat cagattgcct ttacttataa
    28321 tgacaacatc aacggcagcg tggaaaacat tgcagggatt acgaacaaag cagggaatgt
    28381 gttaggaatg atgcctcatc cagaacgtgc agtagaagac ttgctaggat cagaagatgg
    28441 gaaacggttc tttgcttcca ttttaaaaaa cttcggaaag gtgaccgtat agatgaaaac
    28501 attgaaagaa ccgacagctg aagagatcaa gaaaaaaaga atctatgccg aatggggatt
    28561 gactgatgaa gaatatcgaa tgatcgaaga agatatctta ggaagaatgc ctaattatac
    28621 ggaaactgga ctgttttctg ttatgtggag cgaacattgt tcctataaaa attctaaacc
    28681 ggttctacgc aaatttccta ctagtggacc tcatgtgttg caaggccctg gagaaggcgc
    28741 aggcatcgtc gacatcggtg atggactagc agttgtcttc aaagcagaaa gtcacaatca
    28801 tccttcagcc gtggagcctt atgaaggagc tgcgacagga gttggcggaa tcatcagaga
    28861 tattttcagc atgggggctc gtccaattgc gttgctagat tcattgaggt tcggcgaatt
    28921 agataatcca agaacgaaat atttactaga agaagtagtt gcaggtatcg gcggatacgg
    28981 caattgtatc ggcataccga ctgtaggcgg cgaaattgca tttgatcctt gttatgaagg
    29041 aaacccatta gtcaatgcaa tgtgcgtagg tttgattgaa cacaaacata tccagaaagg
    29101 gcaagccgct ggcgtcggta attcgatcat gtatgtaggt gcaaaaacag gtcgcgacgg
    29161 tatccacgga gcaacttttg cctcagaaga atttaatcaa gaagaagaac agcaacgttc
    29221 tgctgtacaa gtaggcgatc cttttatgga aaagctgctg cttgaagcgt gtttggaatt
    29281 aatcttgcat cattcggatt ctctaatagg tatacaggat atgggagcag caggacttgt
    29341 ttcatctagt gcggaaatgg cttcaaaagc tggctcagga cttatattga cattagatga
    29401 agtcccacaa agagaaactg ggatgacacc ttatgaaatg atgttatcgg aatcacaaga
    29461 acggatgttg atttgtgtta aatcaggaga ggaagaaaaa atccaagaac tatttcagaa
    29521 gtatgatcta gatgcagtca ctatcggtaa agtaacagac gacgggcaat atcggctata
    29581 tcatcaagga gaagaagtag caaatctgcc agtcgatgca ttagctgaag atgcaccagt
    29641 ttatcataaa gaaatgaaag aaccagctcg aatcgtagaa tttcagcagt tagctcctta
    29701 tcagccagtt atcgaagaac ctggacaagt attgcttgat ctgctgcagc aaccgacgat
    29761 tgcttcaaaa cgttcaatct acgaaactta tgattcacaa gtacaaacca atacagttgt
    29821 ccgtccagga agcgatgctg ctgtgatgag ggttagaggg acgaataaag cgttagctat
    29881 gaccacagac tgcaacgcgc gctatctgta tttggatcca aaaatcggag gacaaatagc
    29941 agtggcagaa gctgctagaa atattattgc tagcggtggt aagcccttgg cgatcaccga
    30001 ctgcctgaac tatggttctc ctgataaacc agaagttttt tgggaattat cgacttcagc
    30061 tgacggaatt gctgctgcgt gtgaaacgtt aggaactcct gttatttcag gaaatgtctc
    30121 tttatataat gaaacggatg gtcaagcaat ctatccaaca ccaatgattg gtatggtcgg
    30181 attgattgag gatcataaac atatcactac tcaggaattc aaaaaatcag gggatctaat
    30241 ctatatatta ggtaagacgt ttgcagattt tgatggcagt gaattacaaa agatgcagct
    30301 tggaagaatc gaaggtgtga tccgaaactt tgatctatcc attgaaaaga gaaaccaaga
    30361 gttagtattg acagccatcc aaaacggatt gattgaaagt gctcatgact gttcagaagg
    30421 tggcttggca attgcactgg cagaatcagc tttcaaacat cagctaggga tatctgtaca
    30481 gtttgaattg tcctctgctc aattatttgc agaaactcaa tcaagatttg tgttgacagt
    30541 cgcaccggaa aataagacac gattcgaaga aatgatggga gatgctgctg ttttagcagg
    30601 taaggtgacg gatgaagcga taatcgaaat ttcagcaact gacggtcaga tcaaaataga
    30661 aactgcagta gcaagaaagt gctgggagga tgcaatcgta tgtcttatga agtgaagagt
    30721 ttaaatgaag aatgcgggat cttcggtgtt tggggtcatc cagacgccgc tagtgtggca
    30781 tacttcgggc tccatagtct tcagcatcga ggacaagaag gtgcaggtat cgtgtccaat
    30841 gatcgaggaa agcttaaggg gcatcgagat ttggggctgc tttctgaggt attcaaagat
    30901 caaagaaaat tgcagcagtt aacaggagaa gctgcaatcg gacatgtacg ttatgcaaca
    30961 gcaggcaatg gaagtgtgga caatattcag ccttttctat tcaaatttca tgatcagtcg
    31021 atcggcttag cgcataatgg taacttgatc aatgctcgtt cccttagaaa agagttagag
    31081 gaaaatgggg cgatcttcca ttctaactcc gatacggaga tattgatgca tttgatccga
    31141 cgaagtgaag aaacagattt tttaaataaa ttaaaaagtg ctttgaatga agtcaaaggt
    31201 ggctttgcct atttattaat gacagaaacg gcaatgattg ccgctttgga cccaaatggt
    31261 tttcgtccat tagcaatcgg acaaatgaaa aacggagcat acgtgattgc ctcggaaact
    31321 tgtgcattag aagttattgg ggcacgtttt ataagagatg tccagcctgg tgagatagtg
    31381 atcatcaatg acgaaggaat ccagattgat tgctttacga aggacacgca gctttctatt
    31441 tgttcgatgg aatatatcta ttttgccaga ccagattcaa acatcgcagg gatcaatgtg
    31501 catactgcac ggaaaaatat gggacggaat ttagcgaaag aatcgccagt aaaagcagat
    31561 atggtcgttg gcgtacctaa ttcttctttg tcagccgcaa gcggttatgc ggaagctagt
    31621 ggaatccctt atgaaatcgg attagtaaaa aatcaataca ttgcccgaac atttatccag
    31681 ccaacgcctg agctaagaga gcaaggtgtt cgaatgaaat tatctgctgt acgaggagta
    31741 gtggaaggta aaaaggtgat tatggtcgat gattcaattg ttcggggtac gacaagcaga
    31801 cggatcgtcc atcttttgaa agaagcaggg gcaaaagaag tccatgtgag gattgcttct
    31861 cctcctttga aatatccatg tttttacggg attgatattc aaacgcgtaa agaactcatt
    31921 gcggcaaatc acacgattga tgaaatcaaa gaatgtatcg gtgcagattc tttaggtttt
    31981 ttaagtgaag agggtctgat cgaatcgatc ggtctggaaa aagatgctcc gtattcagga
    32041 ttgtgtatgg cttatttcaa cggagattat ccgacgccgt tatatgacta tgaagaaaaa
    32101 tatcaggaat cactaaaaga aaaagtatcg ttcttttaaa agcagaccaa gaagggagta
    32161 tatgaattca atggcaaatg catacgcaaa agctggcgta gatgtcgaag ctggatatga
    32221 agtagtagaa cggatcaaga agcatgtcaa aaaaacggaa cgtttaggta tgatgggcgc
    32281 tataggagga ttcggcggtt gttttgattt atcggtcttt caattaaaag agccagtcct
    32341 tgtctcgggg accgatggcg tggggacgaa attagcagtg gcaatcgaag cagacaaaca
    32401 cgatacaatc gggatcgact gcgtagcgat gtgtgtcaat gatatcttag cacaaggcgc
    32461 agagccgtta tatttcttag actacttagc aacggggaaa aatgatccgt tacgtttgga
    32521 aaatgtagta aaaggtgtag cagatggatg tgttcaagga aatatggcgc tgattggcgg
    32581 agaaacagct gaaatgcctg gtatgtactc aggtaatgat tatgatctag ccggatttgc
    32641 tgtagggata gctgaaaaat ctcaattatt gacaggagaa acgatcaacc caggagatgt
    32701 gttgcttgga atagcatcta gcgggattca ttcaaatggg tactctcttg ttcgcaaaat
    32761 tttctttgag cagcagcagt tttctttatc atcaaaactg ccagaattga aaaaaacact
    32821 tggcgaagaa ttgcttgagc caacgaaaat ctatgtttct tccttgcttc cgctgatcaa
    32881 agaaaagaaa ataaaaggag ctgcacatat cactggtgga gggtttgtag aaaatatccc
    32941 gcgaatgtta ccagaatcta tggctgcaca aatcaaactt ggctcatggc cgattcttcc
    33001 aatattcggc tgtttacaaa aatatggtga aattcctgag atggagatgt atgaaatctt
    33061 caatatgggc atcggtatgg tcctggcagt ccaagaatcg aaagtaaaag aaatagtaca
    33121 cgaaattgag caagctggcg aaaaagttta tgaaatcggg aaagtgatcg ccaaagaaaa
    33181 acatcgtgtg gtgtttatag gagaagaacg atgagaatcg ctgtttttgc ttctggaaac
    33241 ggaagcaact ttcaagcgct agccgattat ctatcgaaaa aaggattgga atcttcgatt
    33301 gactggttgt tttgtgatca gcctgaagca tatgttttga aacgtgcaac tgcgttgagt
    33361 gtaccggcag attgtttttc acctaaagaa tttgattcaa aaaaagagta tgaagaagca
    33421 attttgcata aattgaaaga aaaaaagatc gatttgatcg tattagctgg atatatgcga
    33481 atcattggac cggtattgct gaaaaattat gataaaagaa tcatcaatat ccatccgtct
    33541 cttttacctg ccttccctgg tttgcatggc atacgtgatg cctttgaagc tggagtaaaa
    33601 gaaacgggtg tcacaattca ttatatcgac caaggtgtag atactggacc aatcatccgg
    33661 caggaaaaag tgagaattga acaggaggac acgttcgatt ctttagaaga aaaaatccat
    33721 agagtagagc atcgaatcta tccagaagtg attagtgaaa ttattgaaaa tggaggctat
    33781 ctgaaatgac aagagcattg atcagtgtat cagataaaac tgggattgta gcattcgccc
    33841 aaggtttgat tgaacaagga gtagaaatca tttcaacagg aggaacaaag catgcgttgg
    33901 ataaagctgg gatcaaaacg ctttcgatcg aagaagtgac agggtttcct gaaatgatgg
    33961 atggccgtgt caaaacactt catcctaaaa tccatggtgg tcttttagga agacgcgatc
    34021 tccctagcca tatggaggca atggaaacgg aaaatatcca accaatcgac tttgtttgtg
    34081 tcaatttata tcctttcaaa gaaacgatta tgaaaccaga tgtcgaaata gcagaagcta
    34141 ttgaaaacat tgatatcggt ggtcctagta tgctccgtag tgcagcaaaa aaccatgaat
    34201 ttgtcacctc tgttgttgat ccatctgact atgaagaagt actttcagaa ttacgtcagt
    34261 atgggcatac ttcactttct ttcagacaaa aactagcggc aaaagtcttt cgtcatactg
    34321 cggcatatga tgcactgatc gctcaatact taacggattg ggtggaagaa aaagaaccgg
    34381 aaaaattgac acttacgtat gaacgaaaac aaacgcttcg ttacggtgaa aatagccatc
    34441 aagccgctgc cttttatcag tccgctcttc tggaaaaata ttcgatcgca gcagcaaaac
    34501 aactccatgg gaaagaactt tcttataata atatccgaga tgcagatgct gcgctacgga
    34561 tcatgaagga atatgacgag ccaacggtag tcgccttgaa acacatgaat ccttgtggta
    34621 ttgggacagc atctacgatt ttagaagcgt gggaagcagc gtatgaagca gatcctgttt
    34681 caatctttgg tgggatcatc gttttgaatc gagaagtaga tctgccaaca gcagaacaga
    34741 tgcataagct gtttctagaa atcatcattg ccccttctta tacacaagaa gctttggaat
    34801 tactgcaaaa aaagaaaaac atccgattac tgacagtaga ctttgcgcag gaagaaaatc
    34861 aaaatcgaga agaaacggtc tcagtcttag gtggacttct tgtacaagat caagatctgg
    34921 caatagaaaa tgaagaagag tggaaagtcg tgacaaaacg taagccgact ccagcagaag
    34981 caaaagcatt agcgtttgct tggagagctg tcaaacatgt gaaatctaat gcgatcgtac
    35041 tggcaaacga acgtcagaca gtaggaatcg gagccgggca gatgaatcga gtcggttcag
    35101 taaaaattgc gattgaacaa gcgcaagaga aaatggaagg tgcagtttta gctagcgatg
    35161 cttattttcc aatggatgat tcggttgaat atgcagccaa acatggtata aaagcaatca
    35221 ttcagccagg aggaagcatc aaagaccaag attctattga tatggcgaat aaatacgatc
    35281 tagcaatggt ctttactgat gttcggcatt ttagacatta gagaggagtc ggataaatga
    35341 acattttggt gatcggtgca ggtggtcgtg aacatgcaat aagtaaaaaa cttttagcca
    35401 gtccgaaggt ggatacggtg tattgtgcgc caggaaatcc ggggatgaca aaagatggta
    35461 tcaattgtgt cgctatcagt gagcgagatc attcagcgtt aatccatttt gttaagaaag
    35521 aggctattga ttggagtatc gttggtcctg aagttccttt gttaaatggg atcgtcgatg
    35581 attttatggc ggaaggattg aaaatcttcg gtccgacaaa agcagcagct ctgatcgaag
    35641 gttcgaaaag ttttgctaaa caattaatgg atacttatca aatcccgaca gctaaatcca
    35701 gaagttttga atgttacgaa aaagcagcag cgtatatcaa agaaaatggt gcgccaatcg
    35761 tagtgaaagc ggacggctta gcagctggga aaggtgtagt cgtggctacc acagtgaagg
    35821 aagcgctgga tgcggcagaa caaatgttga accagcatcg atttggggat agtagcaaaa
    35881 aaatcgtcgt agaagagttt ttggcaggag aagaattctc ccttttggcg tttgtccgta
    35941 ataccaaggt gtatccgatg gtcatttcgc aagatcataa gcgggcattt gataacgata
    36001 aagggccaaa tacaggcgga atgggtgctt attcaccagt accgcaaatt tcaaaaaaga
    36061 tgattgaaaa agctgtggaa gaagttttaa aaccagcggc tttgggaatg aaagaactag
    36121 gacgctcttt cacgggtgtt ttatatgcgg gtttgattgc tacgaaggaa ggaccaaaag
    36181 tgatcgaatt caatgcgcgt ttcggtgatc ctgaaacgca agtcgttctt tctcggttga
    36241 agagtgactt tgcgcaagta atcgatgatt tattagagaa tcgtacagta gagttaaagt
    36301 ggcagcaaga aggatacaat gtaggcgttg tggttgcagc tgccggttac cccgaagaat
    36361 atgagacagg aatggttcta ccagattttc tggaagaaga gctttctgtt tactatgctg
    36421 gagtttcctc aaataacggc aaactagttg gagcaggagg acgtctttat ttagtagaag
    36481 catacggtga gacgatcaaa gaagcacaga aaaaaattta caccgcgtta atggataaaa
    36541 cgacagatgg aacattttat cgtacagata ttggagaaaa agctgccaat agcgaactga
    36601 tcgtgaagta aatcagctgc ttccatttat aaaaaataca acaaacataa tcggaagcgc
    36661 cgattttagt gaagcctttt cataaagagt gtggtacact tttgttgtgt atataaataa
    36721 ggaggagttc agatgaaaaa aattcaatta ggtacttctg atcagaaagc agctgcagtg
    36781 atcttaggct gtatgcgtat caacaatgcg gaagatccag taaaagtgat tgagacagct
    36841 tatgaaaacg gcattgattt ttttgaccat gcagatattt atggaaacgg tgaatgtgaa
    36901 gaaatctttg cagatgctct agcaaaaaca tcaatcaaac gagaagatct ttttattcaa
    36961 acaaaatgtg gaatcgttcc aggtgtgatg tttgattttt ctaaaaaaca tatcattgaa
    37021 tctgtagaaa aaagtttgac ccgtctgaaa atggattatg tggatgcttt acttcttcat
    37081 cgtccagata cattggtaga accagaggaa gtagctgaag cgtttgatga attggaacgg
    37141 tcaggaaaag ttcgttattt tggcgtcagc aatcaaaaac caatgcaaat cgaattgctg
    37201 aaaaaatatg ttcgtcaacc attagtagct aatcagctgc aatttggctt gaaacatact
    37261 ggaatgatcg atcaagggat tcatgtcaat atgacagatg aagcaagtat cgatcatgac
    37321 ggtagtgtac ttgattattc ccgattgcat gatatgacga tccaagcatg gtcaccatac
    37381 cagtatggat tttttgaagg tgtatttatc gggaacgaaa aattccctga attaaaccaa
    37441 acattagaag aactagcagc taaatatcaa acgacaccaa ctggcttggc ttctgcttgg
    37501 attttacgcc atccagccaa tatgcaagtg atcgccggta cgatgaacgt agggcgaatc
    37561 aaggaaatcg ctcaagcttc agaaattaat atgtctcggg aagattggta cgaattgtac
    37621 cgtgctgctg gaaacatttt accttaaaga ttgtcataag caggtcaaaa gggctataaa
    37681 ttaaaaagac agaataactt gaaagctgtt ttcagcttcg agatattctg tcttttttta
    37741 ttgcaaatgg aaagagtatc attgtacgga tttcgtttat tagttatttt tgtttttaag
    37801 gaaaagctgt gtccgatatt cttttggaga aatcccttgt tttttcttaa ataaataact
    37861 gaaatagtta gggtcattat aaccaatttc aaaagcaata tctgaaatac gatagtctga
    37921 ttgctgcaat aatttttttg ctaattcgat tctttgttct gtcagataat cgatgaatgt
    37981 ttggttaaac gattgagaaa aaatcgtact gaaatgagag ggacttaatg caacttgttg
    38041 agcgacccaa gttaaggaca tatctggatc ggtaaaattt tctttgataa aatccaatgc
    38101 atggttcaaa attggctgat atttgttcct tgaaggctgt gagggcagag cagttaagaa
    38161 agaaatgaac atataaactg tcgaacggta aagcgagtcg tcggcgctga ttgctaagcg
    38221 ttgactggtg gatgtgcaat gaaccatttc ttgcgggatt ttcttttctt tcaggcagac
    38281 atgctggagt agcttagtca agacaaataa acgatataat cgtgttcgag ttggtgtgcc
    38341 ttgtggtttc gtcaagtgtt cgatgtactt agatacttcg tttttcgata atgaagacaa
    38401 atcaagtgag aggtcaaagt aaaagacaga atcaactgga agtttcgctt cttgaatcaa
    38461 atcctcatag ctgataattt gttctttaag ggaactggcg gtttcaagta tcatctgcgt
    38521 gatctcaaaa gatgcaggta attcgctgat tctttctgcg acaggaccaa tggcgacaga
    38581 aaaattattt ttttgtccct tttctaattc atggaccaac gtttgggcta cctgataact
    38641 ggtctcgatc acattttctt caagagaatc aactattaaa aatttgatga atcgagagga
    38701 aacagcagag aagatcactt gttgattctc cctaaataaa aacttcaaat aatcactgag
    38761 atgagcgtaa tcttcaaagt ctgtactgac ttggtttgct gctaataata cggtcatttt
    38821 tttccctgct aaagagcgat tgaaagattc agcaagtttc aatgtttcac tgatcgtaag
    38881 tgagcctttg aacaaaccgt tcaagaaatg attttttttc aattcgaaaa caagatcaga
    38941 agaagtgtgc gtttcttttt gctgcaaatc taaagcagtt actgcttttt ctattgcttg
    39001 tttcaattcg ctacttttaa tcggtttaag cagataatca tctgcctgta cctggattgc
    39061 tcctttcata taatcgaaat cgtcgaatcc actaataaat atgagacgag tccaaggaag
    39121 tagtttcctt gcttcttttg aaaaagtcaa cccgtccatg aaaggcattt ggatatcagt
    39181 cagaatgatg tcgggctgta agtcgataat tgaagacagt gccatctcac catctccagc
    39241 ttcgccgaca taagtgattg gataataaga accaaggaca gttatttgtt cccgcaagtt
    39301 attgcgaatg aggtattcgt cttcgatgat aaaaacttta tacatgtttg tttcctccaa
    39361 ttgttttggg agatagaggg atcatcagtg tgacagttgt tccttctcgg tatttgctgt
    39421 caatcgtgat aggtttttga tcatttccgt aataaagaag gagacgcttg tttacgttgt
    39481 aaagtccgta gccgatactg aaatctgaat cgatcccttt ggctaactct ttacgtattt
    39541 ctgccagttt ttctgcaggg atgccaatcc cgttgtctat aactttgaat tggatcatct
    39601 catcaatttt gcatgtggta atacgaataa atcctttgtg acggacgtac ttcgttccat
    39661 ggtatacagc attttcaatc aatggctgca agatcatttt taaaatagaa acaggaaaaa
    39721 gtgtttctgg tatatcgatc ttatagtcaa gcatatggcc gtatcttatt tttaagatga
    39781 ctagataatc attgatatgg ctaatttctt tttcaagagg aatccagtct tgtcctttgc
    39841 ttaaagacgt acgaaaaaag ccggaaagtg catgggtcat agtcagagct tcttcaaagc
    39901 agtcttgttc gatcaaagaa ataatggcat ccaaactgtt ataaataaaa tggggagtaa
    39961 tctgagcttg caatacacga acttcacttt gtgctaaatg gtattgtttc aaagcgttct
    40021 cccgtaaaga ctggtttaga tcttccgcca ttttattcat gctagcggta agtgtttgta
    40081 attccagtgt ttccggcagg gttaaacgaa agccaaggtt ccctgaagaa agttgttctg
    40141 acatctcgat cagctgattg atcggttctt ggacctttct ctctaaaaag cgacgattct
    40201 ttttcgcata gaaaaaaatg gtactaagaa ctccaagttc cactattgtt aatagaatga
    40261 gcgagcggac aagtgtcgta ttttttttgc ttgctaagtc gatctccaca cgaacaaaat
    40321 cttgtaagat atctgacagc agttgagtga cagaatctac ctgattgatg atctcttcat
    40381 tggtatccat aaaatctttt gccaagatat tattgatcag ctcgttttga taatcttcca
    40441 gtgtatcgat cgtacgtagc gatacatcta acgtactaat ttctttaagt gtatttgtat
    40501 tatttttgat ctctcgaata tcattcctaa gttcttctag gatattttcc tcattgaact
    40561 catctacggt tacttggcca gaaatcaagt cccacatact ttccaaaact ttttgatcaa
    40621 ccttcaaagc aattgcatct gctttttgga tatttgcgat cgtctgttga taaatcaaaa
    40681 gctgttgcga atataacagt atgctcagta gcaaagggat aacagcaaat agaagcatgg
    40741 ctttgtttgc ttgggcaaaa atctgtttta tgcttaaatt ttttgtcatg atacttctcc
    40801 agtcaataat ttctggttgg gatgttagct atattctgct gggaaaagac agtctcggaa
    40861 ataaaaatat ctttaggtaa tgtttttcct gtttgaatat ttccaaaata acgagtaacg
    40921 gtattggcta cataccagcc gacatcaggt ttacactcga ctacacaatt gacgattcct
    40981 gagcgtagct tctcgatcat atcttcttgt ccatcaatag ttacaatgat caaatcttct
    41041 ccaggtttga ttgatgtttc ttttatcgca gctaaagcac cgtgagtcat ttcatcacta
    41101 tggctgtata atacatcgat ttcggataaa tcatttgttt ctaaatatcg tttcatctgg
    41161 tcttttcctt tcatccgaat aaaatcaccg acaattcggt gaacaatagt catcggaggt
    41221 ttatggtcga tggttccatc tttttttagg gttggtttac gattgattga ttccaaaaat
    41281 ccttcggagc gtagcttggt cggtgtggag ttctctgaac cagcaagttc taggattctg
    41341 atttcctgtt tctttgaatt tgtgaagtga ttgctgacaa ataaacccgc ccgatttcct
    41401 tctgctttga aactggggcc aatgtgggtg acgaaaagct cgggatcatt cgtttgaata
    41461 tgtctgtcga caagtatgac agggatccct gctcgctttg cttcttttaa tacagagtcc
    41521 cacccggatt cttgcaaagg agtaaaaatg atcatatcca ctttataggc aatgaacgtc
    41581 cgtatatctt ggatttgccg ttcctgattc atgtagccat ttcggtagag aacttggtaa
    41641 tcttcttttt caagcgcagt gataattgat tcagtatgct ttttccgcca gctactctcc
    41701 gttcctgatt gcgaaaaacc aacgactagt gggtcaggtg cttcctttgt attaggtgta
    41761 caccccatca tttggtaagc gattacaaca agggctaaaa aaaataaaac acgtttcact
    41821 ttaaatcact ccatctggat ttgttagtac tatcttatca gataaaaggg aaaatcaaag
    41881 tcaaaaggaa tcaatcgaaa aaaatccaag aaacttaaaa aaatccaaga caaaaaaagt
    41941 acacctaaaa aatttaaagt aaatcccata aaaaaatcaa tgttaacgct ttctttatta
    42001 gctatactcg gtgtatcaat tgaaatgggg gataaaaaat gaaaaacaaa tttagaaaga
    42061 ttgttgggac tatcgctatt ttgaccgcaa gcattctatt aagtgcgtgt ggcaattcag
    42121 gatctgcaga tgatagtacg gggtatgtag gaatcgctat gccgaccaaa tcagcagaaa
    42181 gatggatcgc tgatgggaac aacatggtct cggaattaga aaaactgggt tataaaacag
    42241 atcttcaata tggagaagat aaagtagaaa atcaagtagc acaaatagaa aatatgatta
    42301 caaaaggggt agatacactt gtgattgctt ctatcgatgg ttcggcttta actgatgtgt
    42361 tagctaaagc aaaagaagca gatatcaagg tgatcgccta tgatcgttta ctgatgaatt
    42421 cagaaaatgt cgattattac gcgacttttg ataattttgg agtaggtgta tcgcaagcag
    42481 cttatattga agagcactta ggattgaaag aaggaaaagg accattcacg attgaattat
    42541 ttggtggctc gcctgacgac aacaacgcac taatcaatta caacggagtg atgtctgttt
    42601 tacaaccata tatggacaac ggtcagttgg tcgtgccatc tggacagacg agtttcagtc
    42661 aaatcgctac gcttcgctgg gatggttcta cggcgcaagc aaggatggat aatctgttaa
    42721 gcgcaaatta tactgaccaa acattggatg ctgttttgtc gccatatgat ccaataagtt
    42781 taggaatcat ttcgtcatta aaaggcgtgg ggtatgggag tgaaagtaaa cctttacctg
    42841 tgattactgg acaagatgcg acagttgctg gcgttaaatc gatcattgcc ggggaacaaa
    42901 cacaaacgat ctttaaagac acacgtatct tagcaaaaaa tacgattgaa atgatcaaag
    42961 caatcagtga tggagaagag gttccggtca atgatactga aacttatgat aatggggtga
    43021 aaactgtccc tacgtattta gcaaatacag tatctgtcga taaagataat tatcaagcag
    43081 agctgatcga caccgattat tataaagaat ccgatctgaa aaattaagag gaaccaaaca
    43141 aggaactaaa gagatctaag tcagagagag gataagaatt atggcggatt gtattttaaa
    43201 aatggcagat atcaccaaaa aattttctgg aatccgagcg ttagattgcg tgaatctgat
    43261 gattgaacgt ggggaaatcc atgcattgtg cggagaaaac ggagcgggaa aatcaacatt
    43321 gatgaatgtc ttgagcggct tgtatccata tggctcctat gacggggaaa tcttttatga
    43381 tggaaagctg tgtcaattta aagatttgaa agatagtgaa gaaaaaggca tcgtcatcat
    43441 tcatcaagaa ttagcactaa gtccttatct ttcaataaaa gagaatattt ttctcggaaa
    43501 tgaacaagca gaaagaggcg tgattgattg ggaactgaca gaaaagaaaa catatgaact
    43561 gctgaataaa gtcggtttgt ctatcgaccc gaatcttcta gtatcccaga ttggcgtagg
    43621 gcaacagcaa ctagttgaaa tagctaaagc attatcaaaa tccgtccgtc tgttgatttt
    43681 ggatgagcct accgctgcat tgaatgaaga agaaagcgcg aatttgctga gattgatccg
    43741 agagttgaaa gagcaaggtg taacctccat cattatttca cataagctga atgaaatcgt
    43801 ggatatcgca gatcgaatca ccattttacg ggatggacag acgattgaga cactagaaaa
    43861 tgaaatgatc agcgaagagc atattattcg cggaatggtg ggcagagatt tagttgatcg
    43921 ttatcctgaa agaaaaccgg atattggaga agtttatttt aaagtagaag actggacggt
    43981 ctatcaccct cttgatcacg agcgaatcat aaacgaccac atcaacttgc atttgaaaaa
    44041 aggagagatt gtaggaatag ctggactgat gggagcgggc agaactgaat tcgctatgag
    44101 tctatttgga cattcttatg gaagcaagat atccggacgt atattaaagg aaggggcaga
    44161 gatatcagtt aagaccgttc cttcagccat tgcaaacgga ctcgcttatg tctcggagga
    44221 ccgaaaatct ttaggactga atctgctgat ggatattcgg gaaaacgtga cgctgagcag
    44281 tttggataaa ataagtcaaa aaggagtatt agataaagaa aaagaagtaa ctgaggcaga
    44341 aaatttccgg aaaaagatgc gtataaaaac aaactccatc catcaaattg tcagtagtct
    44401 tagtggtggg aatcaacaaa aagtcgtttt atccaagtgg ctgatgacac aacccgatat
    44461 tctttttttg gatgagccaa cccgtgggat agatgttggg gcaaaatatg agatctacac
    44521 gattatcgaa gaaatggcag caaatggcaa agctgtctgt cttatctctt ctgaacttcc
    44581 tgaaatatta gggatgtgtg atcgaatcta tacgatgaac gaaggacgta ttacaggaga
    44641 agtctcgcga gaagatgcag atcaggaagt cttgatgaaa ttaatgacga aagaagaggt
    44701 gggttaagtt ggaacaggca aaaaatctta cggataaaaa agagaaaaaa ctaaatttga
    44761 aaagtagagc agctgatctt tttggtcgct acagcatggt gatcatttta gcgggactac
    44821 ttattgcgtt tcaattgatg acggatggta ttttctggcg tccattgaat ataactaacc
    44881 ttgttctgca aaatagccat atattagtac tagctgcagg gatgttatta gttgtgctct
    44941 taggtcatgt ggatttgtcg gtagggtcag tgatggcgtt tgttggtgca attgcgggca
    45001 tgatgatggt caatcgccaa atgaacccgt ggatgacagt tatcgcatgt cttgcagtag
    45061 gcgggctgat cggcgcatgg caaggttttt gggtagctta tgcagggata ccggcgttta
    45121 tcgtgacttt agcaggattg ctgatgtttc gtggactgac acaagttgtt ctaggaggcc
    45181 aatctctagc accttttcca gctgttttcc aaaaaatgtc tacgggttat ttaccagact
    45241 taagcgaagg aagcagcctt cacttcttga cattgatttt aggaatgatc atcacgatcg
    45301 tgttagttgt tggtcaatgg cgaacaagaa atcgtcggaa aaatcattta tttgaagtgg
    45361 aatctccaac agctttcttg gtaaaggcgg tatttacagc agtcgttgta ctaggaataa
    45421 cttatatctt tgcctcttat caagggtatc cgattatttt gatcattctt ggtttgatcg
    45481 ttgcagcata tgggtttttg acaaataaaa cagtagcagg tcgacagatt tatgcgacgg
    45541 gcggaaatcg aaaagcggct gaactatcag ggatcaaaac aaagcgaatc acgttctggg
    45601 tattcgtcaa catgggagtc atggcagctt tagctggatt agttttagct gctcgattga
    45661 atgcggcaac acctcaagca ggtacatcga tggaactgga tgcaatggcg gcagtatatt
    45721 ttggtggtgc ttcgacttcc ggcggcattg gaacaatcat gggtgcgatt gtcggtggct
    45781 tggtcatggg tgtattgaac aatggcatgt cgattttagg cgtcggggta gactggcagc
    45841 aggcaatcaa aggattgatc ttgcttttag ccgttgtgct agacttgtat aataagaaaa
    45901 aacgaacaac gtaggagaac gccggttatt cagaaaaaag actgtgacaa gacatttgtc
    45961 acagtctttt tttcgctaaa agcgaacatt cattgtttcg tttgtataga ctaaaaataa
    46021 tatatgtttt tttaccattc gtataaattc tctttttgct cttgattttt ttgcttaatt
    46081 gagttaaatt acatagggcg ctaaaaaaag ccttgattaa attaataatg ttcgtgtttt
    46141 gctggatgtg aacatcagga ggataatgta atgaaagtat ttgattacga agatattcaa
    46201 ttaattccta ataaatgtat cgtaaatagc cgttctgaat gtgatacaac ggtcacatta
    46261 ggtaaacata cattcaaaat gccagtagtg cctgccaaca tgcagacgat tattgatgaa
    46321 acaatcgcag aattcttagc ggaaaatggt tatttctata tcatgcatcg gtttgatgaa
    46381 gcagctcgaa ttccgtttat caaaaaaatg aaaaagcgtg gattgattac ctcgatcagt
    46441 gttggcgtca agaaagagga atattccttt atagagaagt tggcagaaga atcgctaaat
    46501 cctgattata tcactatcga catcgcgcat ggacatgcta actcagttat tgatatgatt
    46561 cagcatatta aaaaatattt gcctgaaact tttgtgattg ccggtaatgt tggtacaccg
    46621 gaagccgtcc gtgaattgga aaatgcgggc gcagatgcaa caaaagtcgg gattggtcca
    46681 ggaaaagtat gtattacaaa aatcaagaca ggctttggga ctggaggctg gcaactggca
    46741 gctcttagat ggtgtgcgaa agcagctcgc aagccgatca tcgctgacgg tgggatcaga
    46801 acacatggcg atattgcaaa atctgtccgt tttggtgcga caatggtcat gattggttca
    46861 ttgtttgccg gtcacgagga atctcctggc gaaacaaaag tcgaaaatgg aatcgtgtat
    46921 aaagaatact ttggttctgc ttcagaattc caaaaaggcg aaaaacgaaa tgttgaagga
    46981 aagaaaatct ggatccaaca caaaggatct ttgaaagata cactagtgga aatgcaacaa
    47041 gaccttcaat catctatttc ttatgctgga ggaagagatc tggaagccat tcgaaaagtt
    47101 gactatgtca tcgtgaaaaa ttcgattttt aatggagaca caatctaaaa aataaataaa
    47161 atattaggat gttgtgaaaa agttgtcctg cattaagtaa taagaacagc caaacaaaaa
    47221 tagttcttta tatgttttgt ttggctggaa ttattatcgc agatacaagc ttttgaacat
    47281 cattattcag aataagggac tgtgataaga tttttgtcac agtcccttat gttttgcaaa
    47341 aaaagttttt ggcggatttt ataattaact tagccatttg gaaataaaaa aacgccacgt
    47401 gaattccatg ttatattgaa gttaccacaa ctctcaatag acaaggatga attcacatga
    47461 cgcaaaacca tgataacaca gtttcacgca agggaaaaca cctaacctac tcagaacgtt
    47521 gccaaatcgc catcctgaaa aaggaaaact attccaaccg tcaggttgcg gatgtcctgg
    47581 gctgcgtgcc gcagaccata aacaacgaaa tcaaacgcgg gaccgttact cagcttaacc
    47641 gccagaatca gaacgggaag gtctacgact attatcatga cgtctacgac gccgatgccg
    47701 ggcaggcggc ctacgagaag aaccgcttga actgcggcag acggccctta tgggccgaca
    47761 cggacgcctt cattgaatgg gcggacgaca tgatgctgtt ggaaaaatgg tccccggacg
    47821 tggtggtggg ctttgccaga aaacacgaat tgttcgaccc ggccattatc cctgggacca
    47881 ccaccctcta tcagtggatc gaccgtggca tcatgaaaac gaagaatatg gacctgctag
    47941 aaaaactgtc ccgcaaaccg aaatcgtccc gtcatggcaa ccgcccgaat aaacgggtgc
    48001 tagggaaatc cattgaagag cgcccggagg cggttggtac ccggaaaacc ttcggccatt
    48061 gggaagtcga cacggtcgta ggaaacaaga ccaagtcgga tgccgtgctg ctgacgttgg
    48121 tggaacggca gacacgcttc gaggtcattc tgaagctggc cgggaaggat gccgaatcgg
    48181 tcaaccaagc ggtccgacag ctgagagaac gggctggtgc gtacttcggc cagctgttca
    48241 agacggttac ctcggacaac ggctcggagt tcgcgggact gcacgatgcc ttgcaggatg
    48301 tggcaaacat ctacttcagc cacccctatg cttcctggga gcgcgggacg agcgaaaacc
    48361 aacacaagct gattcgccgt ttcatcccca agggcagccc aatcggcaac gtgagcgaaa
    48421 accaaatctt gcggattcag cgttggatga acgattaccc gcgaaagatt ttgggctatg
    48481 caacgccgca tgacacgttc gtccaggcat ttaggcagga acggttgacg gcttgacacg
    48541 tgggcccttg acaacgagcc tccctcggca tggcttgtgt ctggaaagct ggccggggcc
    48601 agccagtcgg gagactacca tgcctcagct cattgtcaag ggcccactcg attagaaaaa
    48661 ttagacggtt ggtggtaacg agcaaccggg tcaccatggg tggctaactt aaacttgaaa
    48721 tttacgaaaa aaagtttttc ttttttctcg taaattcggt aaaatcaaaa gaaaaaatag
    48781 acaatagaaa agagaagctt atgtgggaaa aagtagagaa aaaacaagga ccgatttatc
    48841 gtcaaattat ggaacagatt atgagagaaa tcgaaaacgg aaggatcaat cctggaagtc
    48901 aacttgaatc agaacgaaaa ctagggcaac tttttggtgt gaatcgttcc accgttgtcc
    48961 atgcattgga agagcttagg gagcttgggg tactaacgag caaacgtggc agcggatggt
    49021 ttgttaatca gactgaatgg gggaaattcg ctgttccaag aatcgattgg cggcaaatga
    49081 tctctcctcg ttatgagcag acggactggt atgagcaaaa aataagagaa gcaaagcaga
    49141 tccaaaaaac atcgtttctt gatttatacg ctagcgagat gccagatgag cttttgccga
    49201 attttgagtt tccttcttat tctcttgaag aaatcttagc ggaagaaaaa gaaatggatc
    49261 ttttaggcta tactccgtta cgtaacaaaa tcaaacggta tttggaaagt aaatttgaat
    49321 tggcattttc ccccaaccag cttcttgtga ctggtggcgg tcagcaagca atctttttga
    49381 ttttacaaac gattctttca gctggagagg ggatagctgt agagtcacct tctttttttt
    49441 accgattacc tcttttcaaa gcttcaggaa cgcgtttatt tgggataccg atggatgaag
    49501 aagggatcga cttagaaaaa ctgaaagaat cgatccataa aaataaattg aaagcagtgc
    49561 ttatcaaccc gaattttcaa aaccctacag gaaaagtgat gagtcaaaaa cgacgagaaa
    49621 agctagtaga attgtgtcga cagtatcggc ttccaattat tgaagacgat gtatttacgg
    49681 atctttcatt tctaaaccaa gaagaagtcc ttcctatacg aacgattgat ccggataatg
    49741 tgttatacgt cggttcgtta tctagggttt taggaaaaac aacgaaaatc ggttgggtga
    49801 taggacctga gacttttatc tcgcaactag ctcaagcgca agaaatgatg gagttttcga
    49861 tgaatatcct tacacaaatc acagtggcta atgtatttaa tgaagcaatt gatttgaaaa
    49921 tgcaagatgt aagaaaaaag ttgaaagaaa aaagccgact gataagcgct tggggagatt
    49981 cacaatcgtt tttcaagctt cattcaattt taggtggtta ctatgcctgg attacttggg
    50041 atgggaaaaa actagacccc gaactagcag aaaaagtagt agatatgggt cttggaattg
    50101 caccaagtct actttttggg caagatataa atggaatcag aatcaattac agtaggctgg
    50161 atgaaaacca aataccgttg ttttattcaa agatgaaaat tctagaaaag tggttaacag
    50221 aataatttca caaagtaaat agattaaaga atataacagc ttggatttaa atttcaagct
    50281 gtttcttttt tttcggaaaa agaatacgat ataaagaaac taagaaaaaa ccattatttg
    50341 tacgtccgtt cacgatgtgt aaataaaata aatgtttgca acaaaactgt ataacaccaa
    50401 cgtttttaaa tagaaacaga tggttgtaag tttgattaga aaatagaaaa aatgcagatg
    50461 aaatataaaa cgctttcctt attgtgattt tattcactaa tgctgttaat atgcggattg
    50521 gcataatttc ctttcatcgt gaaaatttgt gcttttagct tgtatttctc ataaatccat
    50581 gataatttat taatgaagac ggttaaatag tgatacagtt ttattcactg tttcaatcgc
    50641 agaatgaaga gattttctta tagatgagag aggaatgtgt gacaaatggc aaacaaaaaa
    50701 gcaccgatcg acttccaagc actgctagat gaagtcaatg cagatttccc agtatatcaa
    50761 gcacttgatc aagatggaaa cgtagttaac aaagatttag tacctgattt atccgatgat
    50821 gaactcgttg aattaatgac tcatatggta tggtctcgtg tattagacca acgttcaaca
    50881 gcgctgaacc gccaaggacg cctaggcttt tttgcaccaa ctgctggaca agaggcaagt
    50941 cagctggcta gtgcttacgc atttgataaa gaagatgttc ttcttccagg ttatcgggat
    51001 gtacctcaac tagtaaaaca tggtttacct ttatctcaag cttttttatg gtctcgagga
    51061 catgctgcag gtaattctta tccggaagaa ttgaaagctc taccaccaca aattatcatc
    51121 ggtgcacaat atgtacaagc agccggtgtt gcattaggtt tgaagaaacg aaacaagaaa
    51181 aatgtcgtct tcacttatac tggtgatggc ggttcatcac aaggtgattt ttacgaagca
    51241 atcaatttcg ctggcgctta ccatgcaaat gccgtatttt atatccaaaa caacgggtat
    51301 gcgatctcta cacctcgtga aaaacaaact gcagcaaaaa cacttgcaca aaaagctgtc
    51361 gcagctggga ttcctggtat tcaagtcgat ggaatggatc cgcttgcagt atatactgta
    51421 tctaaaatgg cacgggaatg ggcgatcagt ggaaatggac cagtcctaat cgaaacactg
    51481 acttatcgtt atggaccaca tacactatct ggtgacgatc caacccgcta ccgtacaaaa
    51541 gacacagatg acgattggca gaaaaaagat ccattgatcc gcttccgtaa atatttgacg
    51601 gaaaaaggac tttggtctga agaaaaagaa gaacaagtca tcgaagcaac taaagaagaa
    51661 atcaaagcag ctattgctga agccgacaaa gtaccaaaac aaaaggtttc agatttcttg
    51721 aaaaacatgt tcgaagtatc tccacaatca atcaaagaac aaattgcaat ttatgaagcg
    51781 aaggagtcga aataatcatg gcacaaaaaa caatgatcca agcaatcaca gatgccttag
    51841 cacttgaact agaaaatgac gagaatgtcg ttgtttttgg tgaagatgtt gggaaaaacg
    51901 gaggagtttt ccgggcaact gaaggattgc aggaaaagtt cggagaagac cgagtattcg
    51961 atactccttt agctgaatct ggaatcgcag gattgagttt cggtcttgcg ttagaaggat
    52021 ttcgtccagt tcctgaaatc caattctttg gttttatttt cgaagcaatg gacgaagtag
    52081 tagctcaaat ggctcgtaca agatatcgaa tgagcggcac tagaaatcta cccatcacaa
    52141 ttcgttcgcc atttggtggc ggtgttcata caccagaatt gcattcggat aatttagaag
    52201 gtttgatcgc tcagtctcct ggtattcgag tagtgattcc ttccaaccct tatgatgcaa
    52261 aaggactatt gatttcttct attagaagta acgatccggt tgttttccta gaacacatga
    52321 aactatatcg ctcattccga gaagaagtgc cggatgaagc ttacgaagtt cctttggata
    52381 aagcagcagt tactcgtgag ggaacagatg tatctattat cacatacgga gcaatggtgc
    52441 gtgaagcaat caaagcagca gacaatcttg caaaagaaaa tatatctgtt gaaatcattg
    52501 atcttcgtac agttgcgcca ttagatgtag aaacgatcat tcaatccgtg gaaaaaactg
    52561 gtcgcgtagt tgttgtccaa gaagcacaac gacaagcggg cgttgccgcg caagtagttt
    52621 ctgagatctc agaacgcgct atcctttcat tagaagcccc aatcggacga gtttctgcac
    52681 ctgatacagt cttcccattt ggacaagcag aaaatgtctg gttgccaaat gcaaaagata
    52741 tcgaagacaa agtaaaagaa atcgcggaat tttaagaaaa gaagggacga aacaaagtca
    52801 tggcttatca atttaaatt
//
LOCUS       DAZKNH010000007        51284 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000007 DAZKNH010000000
VERSION     DAZKNH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.007"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..1135
                     /locus_tag="ACG8MG_000392"
     CDS             290..1135
                     /locus_tag="ACG8MG_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0374471.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="ACG8MG_000393"
     CDS             1416..2636
                     /locus_tag="ACG8MG_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0374472.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="ACG8MG_000394"
     CDS             2633..5353
                     /locus_tag="ACG8MG_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0374473.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="ACG8MG_000395"
     CDS             5337..6281
                     /locus_tag="ACG8MG_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0374474.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="ACG8MG_000396"
     CDS             complement(6350..7360)
                     /locus_tag="ACG8MG_000396"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0374475.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="ACG8MG_000397"
     CDS             complement(7540..7884)
                     /locus_tag="ACG8MG_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0374476.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="ACG8MG_000398"
     CDS             complement(7877..8311)
                     /locus_tag="ACG8MG_000398"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFQ0374477.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="ACG8MG_000399"
     CDS             8449..9186
                     /locus_tag="ACG8MG_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0374478.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="ACG8MG_000400"
     CDS             9179..10390
                     /locus_tag="ACG8MG_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0374479.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10594..11376
                     /locus_tag="ACG8MG_000401"
     CDS             10594..11376
                     /locus_tag="ACG8MG_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFQ0374480.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="ACG8MG_000402"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="ACG8MG_000402"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFQ0374481.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="ACG8MG_000403"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="ACG8MG_000403"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFQ0374482.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="comGA"
                     /locus_tag="ACG8MG_000404"
     CDS             14092..15021
                     /gene="comGA"
                     /locus_tag="ACG8MG_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFQ0374483.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /gene="comGB"
                     /locus_tag="ACG8MG_000405"
     CDS             14978..16027
                     /gene="comGB"
                     /locus_tag="ACG8MG_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFQ0374484.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /gene="comGC"
                     /locus_tag="ACG8MG_000406"
     CDS             16024..16302
                     /gene="comGC"
                     /locus_tag="ACG8MG_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFQ0374485.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /gene="comGD"
                     /locus_tag="ACG8MG_000407"
     CDS             16277..16747
                     /gene="comGD"
                     /locus_tag="ACG8MG_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFQ0374486.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16719..17066
                     /locus_tag="ACG8MG_000408"
     CDS             16719..17066
                     /locus_tag="ACG8MG_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374487.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            17029..17454
                     /gene="comGF"
                     /locus_tag="ACG8MG_000409"
     CDS             17029..17454
                     /gene="comGF"
                     /locus_tag="ACG8MG_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFQ0374488.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /gene="comGG"
                     /locus_tag="ACG8MG_000410"
     CDS             17426..17785
                     /gene="comGG"
                     /locus_tag="ACG8MG_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFQ0374489.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="ACG8MG_000411"
     CDS             17957..18964
                     /locus_tag="ACG8MG_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0374490.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="ACG8MG_000412"
     CDS             18989..20173
                     /locus_tag="ACG8MG_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFQ0374491.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /gene="abc-f"
                     /locus_tag="ACG8MG_000413"
     CDS             complement(20610..22193)
                     /gene="abc-f"
                     /locus_tag="ACG8MG_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFQ0374492.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22298..22426)
                     /locus_tag="ACG8MG_000414"
     CDS             complement(22298..22426)
                     /locus_tag="ACG8MG_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374493.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(22771..22914)
                     /locus_tag="ACG8MG_000415"
     CDS             complement(22771..22914)
                     /locus_tag="ACG8MG_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFQ0374494.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23137..23616)
                     /locus_tag="ACG8MG_000416"
     CDS             complement(23137..23616)
                     /locus_tag="ACG8MG_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0374495.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23689..23973)
                     /locus_tag="ACG8MG_000417"
     CDS             complement(23689..23973)
                     /locus_tag="ACG8MG_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374496.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24051..24245)
                     /gene="ssrS"
                     /locus_tag="ACG8MG_000418"
     ncRNA           complement(24051..24245)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8MG_000418"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24317..25603)
                     /locus_tag="ACG8MG_000419"
     CDS             complement(24317..25603)
                     /locus_tag="ACG8MG_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFQ0374497.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25741..26412
                     /locus_tag="ACG8MG_000420"
     CDS             25741..26412
                     /locus_tag="ACG8MG_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFQ0374498.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26378..26866
                     /locus_tag="ACG8MG_000421"
     CDS             26378..26866
                     /locus_tag="ACG8MG_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFQ0374499.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26879..27826
                     /gene="prmA"
                     /locus_tag="ACG8MG_000422"
     CDS             26879..27826
                     /gene="prmA"
                     /locus_tag="ACG8MG_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFQ0374500.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27827..28591
                     /locus_tag="ACG8MG_000423"
     CDS             27827..28591
                     /locus_tag="ACG8MG_000423"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFQ0374501.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28824..31037
                     /locus_tag="ACG8MG_000424"
     CDS             28824..31037
                     /locus_tag="ACG8MG_000424"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFQ0374502.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31048..31494
                     /gene="dtd"
                     /locus_tag="ACG8MG_000425"
     CDS             31048..31494
                     /gene="dtd"
                     /locus_tag="ACG8MG_000425"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0374503.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31665..32924
                     /locus_tag="ACG8MG_000426"
     CDS             31665..32924
                     /locus_tag="ACG8MG_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFQ0374504.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33013..33960)
                     /locus_tag="ACG8MG_000427"
     CDS             complement(33013..33960)
                     /locus_tag="ACG8MG_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0374505.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34354..35646
                     /gene="hisS"
                     /locus_tag="ACG8MG_000428"
     CDS             34354..35646
                     /gene="hisS"
                     /locus_tag="ACG8MG_000428"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFQ0374506.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35661..37430
                     /gene="aspS"
                     /locus_tag="ACG8MG_000429"
     CDS             35661..37430
                     /gene="aspS"
                     /locus_tag="ACG8MG_000429"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFQ0374507.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37539..38414
                     /locus_tag="ACG8MG_000430"
     CDS             37539..38414
                     /locus_tag="ACG8MG_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0374508.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38680..40563
                     /locus_tag="ACG8MG_000431"
     CDS             38680..40563
                     /locus_tag="ACG8MG_000431"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0374509.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40638..40922)
                     /locus_tag="ACG8MG_000432"
     CDS             complement(40638..40922)
                     /locus_tag="ACG8MG_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0374510.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40912..41646)
                     /locus_tag="ACG8MG_000433"
     CDS             complement(40912..41646)
                     /locus_tag="ACG8MG_000433"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFQ0374511.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41775..42398
                     /locus_tag="ACG8MG_000434"
     CDS             41775..42398
                     /locus_tag="ACG8MG_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFQ0374512.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42590..43711
                     /locus_tag="ACG8MG_000435"
     CDS             42590..43711
                     /locus_tag="ACG8MG_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0374513.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43718..46846
                     /locus_tag="ACG8MG_000436"
     CDS             43718..46846
                     /locus_tag="ACG8MG_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0374514.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46865..50059
                     /locus_tag="ACG8MG_000437"
     CDS             46865..50059
                     /locus_tag="ACG8MG_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFQ0374515.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50205..50912)
                     /locus_tag="ACG8MG_000438"
     CDS             complement(50205..50912)
                     /locus_tag="ACG8MG_000438"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0374516.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16783 a         8954 c        10639 g        14908 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatcccaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggttt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg agactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccatggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttta ttttacttga actcattgac tgttttccct ttattttaaa
    23041 cattataaag accgatgagt gcttgccgcg tcttttgttt ttatcacggt tttttgtgca
    23101 aaaaaacctg ccgatagttg gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc
    23161 tggcaattgg ttttctaaat cagttcggac aacaagcaca tcgcaagctg cgtttctaat
    23221 aacatattca gaaacagaac caataaataa acgttctaca gcattcaatc ctgtggctcc
    23281 taacataatc aaatctactt gattatcttc tgggatttct ctggcaataa tttgttttgg
    23341 tgagccatat tctacgacag aggttacatt attcaagcca gctttcttcg cattgctttc
    23401 ataatctgcc aatgtttgct ttgccatttc agtagcttgt tctgcaagca ttccatcaaa
    23461 ggatgaaacg gtttggaaag aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt
    23521 atttcggata gctacattga ccgccttttt aaaagctaat tcagcttcat cagaaccatc
    23581 tacggcaacc atgatttttt tgtattgttg taacataagc attcactcct caagtcaatc
    23641 gtattgtact cttttattgt agaacacttt agggaaaaag agaattattt actgctttgg
    23701 ttcagcaatt ttttttgcaa atcggatact gaaggtggca gaaacaataa ttacgatcag
    23761 taaaaagacc aaggcaataa agcgttgatt gaaaaaagct gttaaaatac ccattacgcc
    23821 taaaacagca tatgcagctc cgtaaaattg taggaatcct tgatttttat catttttgtt
    23881 gatcaataca aaaaagatat cctgtttatt aaataaataa tagctaacga ataataatac
    23941 taaagtggcg ataatcatta aaatcctaat catgaacaaa tcctccaaaa taagcaacaa
    24001 atagaaaaag caatttgcca agagcaaatt gctttattct ttaaaagatg gtaatctccg
    24061 ttaatgccgt tgtttgtcct gtagtattga tcccgcgaat tcaagcaggt gggtcccatg
    24121 gtcgtagctt cgaactacca aatgataagg catgttacca gcagtgacac aagacgggtc
    24181 ccaagatatc aataaaaatg ttcggtcaac acacaaagag gacaattcga acatcacaga
    24241 tttcccacct ctatagtagc atatgaattt aaaaagagca atgagagagc tcgcagaaac
    24301 agaaccgttt tcttgtctag ttttttggag gatgcttttt ccactctttt atacggtaat
    24361 actgttgacc cagagcctgt tcatatttcc ctgtatcttt tggctgataa tactgagcat
    24421 tcttcagttt atctggtaaa tattgctgat tgacccaagc ctgatcaaaa tgatgaggat
    24481 actgataacc cacgccacgg ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat
    24541 gatcaggaac atctccagct ttgccctctc gaatatcagc aagtgctgca tctaatgcca
    24601 tgtaggcaga attggatttt ggcgacaagc aaagatcgac gacgacgtct gctagtggga
    24661 tacgtgcctc aggcaatccc aatttttcag cagctaatac agcgttcact gtacgagccg
    24721 cagcagcagg attacctaaa cctatatctt cataaccgat aaccatcaga cgccggcaaa
    24781 tacttgccag atcacctgcc tcaactaaac gggcgagata atgaagtgca gcatccacat
    24841 cacttccacg aatcgatttt tgaaaagccg aaataacgtc ataatgagca tctccatttt
    24901 tgtcatgggt aagagctttt ctttggacac attcttcaat gatcgacagt gtaagatgaa
    24961 tccttccttc tttatctgaa ggagtcgata gtgtagccag ttccagccca ttcaatgcac
    25021 tccgcaaatc gccgttcgtt gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga
    25081 ttgcttgttg tccaaggccg cgttctttat cccttaaagc atgttcgact gccaactgga
    25141 tgtcttgttc tgttaatggt ttgacttcaa agatctgtgt acggctgcgg atcgctggat
    25201 tgatggtaat ataaggattc tcagtagtcg ctccgatcaa gatgattcgg ccactttcta
    25261 aatgaggaag cagaaaatcc tgttttgttt tatctaatcg gtgtacttca tccaataaga
    25321 ggatcactgt accactcatt tttgcttctt ccgctacaac ttgtagatcc ttttttgaat
    25381 ctgtagcggc gtttagcatg cgaaatgcat aattagtaga gccggcgatc gcacttgcga
    25441 tactcgtttt tccagtacca ggaggtccat atagaatcat ggaagagagc atgcgagcat
    25501 cgaccatgcg gcgtatgatt tttccttccc cgactagatg ctgctgacca acgacttcat
    25561 cgatcgttcg aggtctcatg cgataagcca atggctgttg cataggattt tcctccttct
    25621 tgtttttacg tcccttaatt atatcatatt tagcaagatg cctagaaaga acagccttct
    25681 gttgttttgt tttcatagct tatccaacta atattgtgta tgataaagta ggtgagaaaa
    25741 atgacggata aacaaaagat agatgaattt ttttggaata aaacgacgcc agaagtggca
    25801 cgggatttat tggggatgta tttggagcat gacacaccag aaggaagatt agctggatat
    25861 attgtagacg cggaagcata cctaggtcca gaagacgagg ccgcccatag ctatggttta
    25921 agaagaacac cgcgtgtgcg ggcaatgtat gaaaaaccag gtacgatcta cctttacacg
    25981 atgcatactc atcggatttt gaacatcatt actcagccag aagggattcc tcaaggagta
    26041 atgatacgtg caatcgaacc cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga
    26101 aaaactggtc cagatatcag caacggtccg ggaaaattag tggaagcatt agcaattcct
    26161 caagaacttt atggtcagtc tatagcagat agttcgttac gcttagtttt tgagaaaaaa
    26221 aagacaccaa aaaagatcaa tgctttgcct agaatcggta tcccaaacaa aggtgtatgg
    26281 acagaaaagc cgttgcgttt tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa
    26341 aaccaaatcg agaaagattg gggttggagg aaagaaaatg aaaaagaagg atctattaac
    26401 atatttagat gaaataattg aaaaacaagt gacagactat gatgtagctt tagattggga
    26461 tacaaaaaat catacgatcg agatcgttgt tcgcttgttt gcagaaaata aagcacaatt
    26521 tgagattgat gatgcagaag ggatcgtttc gcaagaacca attatcgaat ttgaagacgg
    26581 cgtacttctt tttaatcctc agaaatctgt ttttgatgaa cgcaattatc tagcagtaat
    26641 cccttatgaa ggaaaaaaag ggttggcaaa atcagtagct gattctttag tggaatatct
    26701 aaatgaagta ttagctcaag ggcaaagcga tttgcttgat tttttggatg aagacgatga
    26761 cgaagctgta ttcgagcttc actgggataa tcagcaacta gcagaattag tagaagtaaa
    26821 acagcaaaat gaagcagata cttatttacc gtacccaagt tattaaggag tgacagatat
    26881 gaaatggaat gaagtaaaag tagaaactga aagtgaagca gtcgaagcag ttgctaatat
    26941 tttgatggag gcaggggcga gtggtgtcgc gattgaagat tctttagatg ttgaacattt
    27001 tcagtcggat caatatggtg agattttaga taaaaatgat tttacatcgc tgaaagaagg
    27061 cgcactagtc atggcttatt ttccggaaac gactttctta ccggaaatct tgccttttat
    27121 caaagatcgt atcacacaat tacctgagtt tggtctagct attggaaaaa ataaagtaac
    27181 agttagtgaa gtagaggaaa gcaactgggc aacagcctgg aagaaatact atcatccggt
    27241 gcgtattacg cgaatgctga cggttgcgcc aagctgggaa aactaccaga cagaagatcc
    27301 tctagaaaaa attatcacac tagatcctgg tatggcattt ggcacaggca ctcatcccac
    27361 gacttccttg acattgacag cactagaaac agtcttgcgt ggtggtgaaa cattgctaga
    27421 tgtgggaact gggtcgggcg tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt
    27481 ctatgccttt gatttagacg aagtagccgt tcgttcggcg aaagaaaata tggatatgaa
    27541 tgaaatagcc aaagatgtcc atgtatcagc taacgatctt ttgacaggaa tcgagattga
    27601 aagtgatgta atcgttgcga acattttagc agatatcatc ttattgatga tcccagatgc
    27661 ttggcgttta ctgaagcaaa caggcacgct gatcgtttca ggaatcattg aagagaagaa
    27721 gcaaatggtt ctggatgcca tgttagaaca agggtttatc gtggatcaaa ttttccagca
    27781 aaaagactgg tatgcaatta ttttgaaaaa accagaggtg gaatagatgc agcggtattt
    27841 tttaaaagaa tcatatgaat caaaagacta ttttgtttta gaaggggaaa actatcacca
    27901 cattgtacgg gttatgagga tggaacccga ggatgaagtt tttttggcat tcaatgatca
    27961 aatagccatt gttgcagaaa tcacggagat tactgaagag gctgtttatc tgaaagaaat
    28021 taaaaaagaa gaaaatcaaa aggaactgcc aagttcgatt accatagcga gtggctatcc
    28081 taaaggggaa aagctggatt ggatcgttca gaaatccact gaattaggag cgcaagcttt
    28141 tcttggtttc ccggcatcag cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg
    28201 acaacaacgg cttgaaaaga ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt
    28261 gccatcagta gagttattgg aaacgaaaca agatttattt gagcggctag atgattatga
    28321 cgtggtcttg gttgcttatg aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa
    28381 aattcttagt gaaacaaaac cagatagtcg aatcttagct gtctttggac cagaaggtgg
    28441 attatcccgc gatgaagtcg attattttgt agaacacaaa gggatacttt gtggattagg
    28501 accaagaatc cttcgtacag aaacagcacc tctttattta ttaagtgctg ctagctatca
    28561 atgggaatta atgaatcctt ctttcagcta acgttcttca ttaataaaag agtatcgaca
    28621 gaaagaattg aaaaaatagt caacaatttg tataatagaa acaattattt atacaatgga
    28681 tacgaaaaat cgtttgaaaa agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa
    28741 ctgcacggat tttgggacaa ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa
    28801 aaaagaaagg gggatcgatt cctgtgacaa aagaagaaat tttaacggga cccggtgtca
    28861 taaagctcgt tagtcaatac atgggtcccg aacatgtagc atttgttgag aaagcatgcg
    28921 aatacgcgac tgctgcacat gacggacagt ttagaaaatc aggcgaaccc tacattattc
    28981 atcctatcca agtcgcaggt atattagcag atttaaaaat ggatccccat acagtggcta
    29041 caggcttctt acatgatgtt gttgaagata cagaaatcac tttagaagat ctgagagaag
    29101 aatttggcga tgacgttgct atgttagtgg acggcgtaac caaattaggg aaaataaaat
    29161 ataaatccca cgaagagcag ctagcagaaa accaccgaaa gatgctgcta gcaatggctc
    29221 aagatttacg agttatcatg gtcaaattag ctgatagact acataacatg cgtacgttga
    29281 agcacctgcg agaagataag caaagaagga tcgctcagga aactttagaa atctatgcgc
    29341 ctcttgcaca tcgtctaggg atcagccgga tcaaatggga attagaagat acagcacttc
    29401 gttatctaaa tccaaaacag tattaccgta tcgtccactt gatgcagacg aagagagaag
    29461 aacgtgaaaa atacgtaagt ggtactgttg aagatattcg aatagcgaca gaagagctgg
    29521 ggatttttgc agaaatctat ggacggccaa aacacattta ttcaatctat cgtaaaatga
    29581 aagatcagaa aaagcagttc aacgaaattt atgacttgct agctattcga gtgatcgtag
    29641 attcaatcaa ggattgttat gctgtattag gagcaatcca cacaaaatgg aaaccaatgc
    29701 ccggcagatt taaggattat attgccatgc caaaagctaa tatgtaccaa tctttgcata
    29761 ctaccgtgat cggtccggca ggaaatccgg tagaaattca aatccgaaca caggaaatgc
    29821 atgaaatcgc tgaattcggg gttgctgcac actgggccta taaagaagga aaaaatgaaa
    29881 aagtagaacc agatggtatg acgaaacaat taagctggtt ccatgagata ctcgaacttc
    29941 aagacgaaag ctatgatgct tctgaattta tggaaggcgt aaaaggagat atctttagtg
    30001 ataaagtcta cgtcttcaca ccaaaaggag acgttactga gttaccaaaa ggatccggac
    30061 cattggactt tgcatacagt atccatacag atatcggtaa caaaaccact ggtgcaaaag
    30121 taaatggcaa aatggtgcag cttgattaca aattgaaaaa cggagatatc attgagatca
    30181 tgacttctcc aaattcattt ggcccaagtc gcgactggtt gaaattagtt gctactagca
    30241 aagcaagaaa taagatcaaa cgtttcttca aagcccaaga tcgagaagaa aatgtgatca
    30301 aaggccacga atccgtggtc aaatgtatta cagatctagg atttacgcct aaagatattt
    30361 tgacgaagaa caaactgcaa gaagcactcg atcgttttaa ttatcaaaca gaagatgatc
    30421 tctatgcagc tgtagggtat ggagaagtta gccccttgac gatggccaat cgtctgactg
    30481 aaaaagaacg taaagaacaa aaaatcgagc agcaaaagca agaagcagaa gaaatcatga
    30541 atcagccgaa aaaagaacct gacaaaatga aagtacgtca tgaaggtggc gttgtcattc
    30601 aaggggtaga aaacttacta attcgtatca gtcgctgctg taatccgatt cctggtgatg
    30661 atatcgttgg ttatatcact aaaggcagag ggatatccat tcatcgtcga gattgtccga
    30721 atgttcagcc tgacaaacca aatgtagcag aacgtttgat tgaagtcgaa tgggaagata
    30781 catcgaatac acgaaaagag tatgatgcag atttggaaat ttacggctat aatcgttcag
    30841 gcttattgaa tgatgtactt caaacagtca atgcgctaac gaaaaatctc aacagcgttg
    30901 aagcacggac gaataaagat aaaatggcga cgatccattt gacggttggt atccagaatt
    30961 tatcccacct aaagagtatc gtggataaaa tcaaagcagt acctgatgtc tacagtgtac
    31021 gccggacgaa tggataggag gacagatatg agagcagttg ttcaacgggt aagtaaagct
    31081 gctgtgtcag tcgatcagca agaaattgga aaaattggct acggcctact tgttttatta
    31141 ggcattcatg aaaccgatac gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa
    31201 atgcgtattt ttgaagatga acaaggaaaa atgaatatta gtgtagaaga tgtgggagga
    31261 caaattttaa gtatctctca attcacactg tttgcggata cgaaaaaagg aaatcgacca
    31321 agctttgtgg aagccgcacg accagaaaca gctattcctt tatacgaatc attcaatgaa
    31381 ggaatcagaa gtcgcggcat cacagtcgct accggacaat tcggagcaga tatgtcggtt
    31441 tctctcgtca atgatggacc tgtaacgatc attattgatt ctaaaaacaa ataaagtaat
    31501 ctggttttca ctagtaaaaa ctatgaaaca cgtcgcttaa gttagactga taaactctaa
    31561 cttaagtggc ttttttgtct gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt
    31621 aagcttaaat tatacaagtt attttttata ggaggttgct gaagatgaaa gaagtaccag
    31681 tagtagatat caaaatcaca gatttaaaaa aattgtatca aaaaggatac aacatgttgg
    31741 aggagttacg ccatgaagcg gacgctcccg tcgtcaaagc aaagattttc aataaagaag
    31801 ctatcacgat ctacggatca tcagcagcaa aagtatttta tgatccaagg aatttcaaac
    31861 gaaaaggtgc tatgccaaaa ttagtattga agacactttt tggtcaaggt ggagtacaga
    31921 ctttggatgg agctgctcat caccatcgga aaaatatttt tatggatcta atgacaccgg
    31981 aaagaatgga agattatcat cgtatcttag ataagaatct gacacaagca cttgaagcac
    32041 agcatggtca atttgaacta tttgatttaa gtaagatggt tttctttaca tctatttgcg
    32101 agtgggcagg tatcaattta tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat
    32161 atcaaatctc tatgatcagc ggaacgttta cttctccgat tgatcacata aaaggtgtcg
    32221 aaaatcgcaa aaaatcagaa aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc
    32281 ctgtggctgg aaaagaaaat gtggcattat atgcttttgc caatgccaca gatttagatg
    32341 gtcagctttt acctctggaa gtggcagctg ttgaattatt gaacatcatc cgtccaacgg
    32401 ttgctctaac tgtgtgggcg gcattgatgg gacatgcact tttcagtaga ccagatttgt
    32461 atcagcaatt aaaaaatgac ttttcgacgt tacaagatcc gtttattcaa gaaatgagac
    32521 gctattatcc tttcttccca atgctacctg cgatttcgct gaaagaagta gaagtagatg
    32581 gttatcgtat accagaaggc agttgggtta ttttagattt atatggaaca gaccatgatg
    32641 aacgaacagt tgaagcgcca gattccttta tgatcaaacg gtatgtaggg aaagcaaaag
    32701 atatttccta taaagaagaa tatgagatga ttgcacaagg cgggggaaat tttcgacaaa
    32761 tgcatcgctg tgcaggagaa tggatcactc ttcatagtct acgtgtgttt tccgatcagc
    32821 tagtaaataa gtttgagttc tctgtacctg aacaagattg gacgattcca ttcaatcagt
    32881 ttccgactta tccgaatagc cgagcgctac tttacaagaa ttagattagc ataaacttat
    32941 cgaaaaaaag gaagatcaaa cagtgctgat cttccttttt tcatattact ataaattgat
    33001 aaggtcaatt gatcaaaatc cacgaaatgc ttcatcaatg gtttgataat cttcattgga
    33061 taaatgcaaa tctaaagctt ggacattgtc agatacttgc tctggacgct tagctccagg
    33121 tataactaca gacactcgtg gattcttcat gtaccaagct aaaacaagtt gcgcaggagt
    33181 ggcttggtat cttttagcaa tggggcgaag tacatcgaca gcggttaaaa tttctctgaa
    33241 acgttctcct tggaaatctg gatttctttt tcgaggatct ccttcaccaa attgtttttc
    33301 ctcaccaagt tcatattttc ctgtcagcaa accggaagca agaggaaaat agggaacaaa
    33361 actgattttg tttttttcaa gataagggaa taattctttt tcagcctgcc gatggatcaa
    33421 actgtattta tcttctacta catcgacata tccatctgca ttcgcttctt tcaattgttc
    33481 taacgtgaag tttgacacac cgactgctcg gatttttcct gcttctttta attcgtgcaa
    33541 tgtagcaact gattcatttt ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc
    33601 gatgtaatca gtttgcagac gttttagtgc atcttccacc gattgtttga gaaactcagg
    33661 agaattatca aatgttcgcc ctttatttgg tacatgagca gcttttgtag cgatgacgac
    33721 acgtgagcga tcatattctt tttccttcag tacctcgcca atcagttctt cggaacggcc
    33781 gtttccgtaa gcataagcgg tatcgatcaa attgatcccg ctattcaaag ctgtacgaac
    33841 tacttgtttt cctgtctcat cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt
    33901 tgctcctaat ccaagctgtt ctgcgtagac ttgtgaatgt ccgattctta tttctgccat
    33961 agctgattcc tcctttttgt ctagtctaaa acaaaatacc aaaaagctga actaaaacgt
    34021 ttttgtgagg ggaaatagtc agatatctct tgactttgag actggttttt agtaggttat
    34081 aagtaatatc gaatgtcaat gaaagagcga gtaagttttt gaaactttac aagagaggat
    34141 catcgaggct gaaaatgatc taaagtttca ttagcctaaa gacactctgg agatggacga
    34201 gtgaaaagcc gtcccgcgta cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat
    34261 taggtggtac cgtgtatgaa tttcattcac ccttgttcat gatgaacaag ggtgattttt
    34321 ttatttacat agctaagaaa gggagagaaa actatgagtt tccaaagacc aaaaggaaca
    34381 aatgatattt tgccaggcga atcagaaaaa tggcagtttg tcgaagaaac agcacgttta
    34441 ttattcagag attatcagta taatgaaatg cgtacaccga tttttgaaca ctatgaagtg
    34501 atatcccgaa gtgtagggga tacaacggat atcgtttcaa aagagatgta tgatttttat
    34561 gacaaagggg aacgccatgt gacgcttcga ccagaaggta cagcaccaat cgttcgttct
    34621 tttgttgagc ataaattgtt cggcccagag tatgcgaaac cgttcaaaac tttttatatg
    34681 ggaccaatgt ttcgctacga acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc
    34741 ggagtagaag catttggatc agaaaaccct gcaactgatg tggagagcat ggtgatggca
    34801 cttgatttct tcaaacagtt ggggatcacg caaatccggc tagtcatcaa ttcattaggt
    34861 gataaagaaa cgcgtcaagc ctatcgtcaa gcattgatcg actatctttt accttttgaa
    34921 tcagagctga gtgaagactc taaacgccgc cttcatgaaa atccattacg cgtattggac
    34981 agtaaagaca aacgagatca aaaatttgtg gcagaagctc catctatctt agattatcta
    35041 agcgagcctg ctcaaaaaca ttttgatacc gttgtttcca tgctggatgc attggctgtt
    35101 ccttatgaaa tcgacagtaa tatggttcgc ggactagatt attacacaca tacgattttt
    35161 gaaatcatga gtgacgcacc aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt
    35221 tatgacaatc tagtggaaga actaggtggt ccttcaacac ctggcttcgg atttgcaatg
    35281 ggtatcgagc gattactctt gactatggag gcagaggaag tagttatccc tgcatttaat
    35341 gagttggatg cttacgtggt cgcactcgga gacgaaacaa atatcgaagc attgaaagtg
    35401 gtccaagcga ttcgaaactt tggtttttca gctgaccgcg acttcatgaa ccgtaaagca
    35461 aaagcgcaat ttaagacagc agataaagcg aatgccaaat tagtattggt gatcggtgaa
    35521 gatgagcttg ctagtcaaac gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt
    35581 tttccattag aagagatata tgaacatttt gatgaagtct atgatgaaat gactttattc
    35641 gagtaggagg aaaaaaataa atggcacaaa gaacgatcta ttgcggatta gtttccgaac
    35701 aacaattaga acaaacagtt acattaaaag gatgggtaca aaaaagacga gaccttgggg
    35761 gagtaatctt tatcgaccta cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg
    35821 aaatcaacaa agaagcatgg gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag
    35881 tgactgggga agttagaaga cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg
    35941 aattcgaagt gattgcaagt gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc
    36001 aaattgaaga tgatcaagca atcagtgacg aaatacgttt gaaatatcgt tatttggatc
    36061 tgcgtcgtcc aaaaatgact aaaaacatca aattaagaaa tgcgacaaca aaagcgattc
    36121 gtcattattt agatgataat gactttctcg atatcgaaac accttaccta gggaaatcta
    36181 caccagaagg cgcaagagac tacctagttc cttcacgtgt ccatgctgga catttctatg
    36241 cgttgccgca atctcctcaa ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt
    36301 attatcaaat cgttcgttgt ttccgcgatg aagatttacg tggagaccgt cagcctgaat
    36361 ttacgcaagt agatattgaa acaacgttct taacagcaga agaaatccaa acttacacag
    36421 agggattgat tgcgaaggta atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc
    36481 ctcgtatgac ctatgatgaa gcaatggctc gctacggcag cgacaaaccg gatacacgtt
    36541 ttgatatgga attgatcgat ctaagcgata cagtcaaaga agtagaattc aaagtcttcc
    36601 aaatggctct tgaaaacggc ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc
    36661 gctactcacg aaaagacatg gatcagttag gtcaatatgt aggacaattc ggagctaagg
    36721 ggcttgcatg gctgaaagta gaagaagacg gattgaaagg tccaattgct aaattcatgg
    36781 gagaagcaac agaagcaatc attaaagcaa cagatgcaaa accaggtgac ttgctgatgt
    36841 ttggcgcaga taaaagtgaa atcgtcgctg ctgcattagg tgctatccgg acacgtttag
    36901 gaaaagaatt aggactaatt gatgaatcga aattcaattt cttatgggta actgattggc
    36961 cacaatttga attttcagaa gaagaaggtc gatatgtctc tgctcaccat ccattcacga
    37021 tgccaaaaga agaagatatc ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag
    37081 cttatgatat cgtcttgaac ggttatgaat taggcggagg ttctatccgt attcatactc
    37141 gtgagcttca agaaaaagta ctagaaactc taggctttac aaaagaacaa atggaagaac
    37201 aatttggttt cttgttaaca gctttagatt acggattccc tccacatggc ggtattgcat
    37261 taggattgga ccgttttgca atgttattag ccggagaaga taatattcgt gaagtcattg
    37321 cctttcctaa aaacggaaaa gcagcagatg tgatgagtga tgcaccaagt atggtttcgc
    37381 ctttacaatt gttcgaattg aatattgatg taacgaatat agaagaatag ccgataaaaa
    37441 ggtaaaaagg atttgtcgaa tacgcttctt ttgacaacct tgatttaatt gaaaaacact
    37501 ttataatgaa gccataacat ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt
    37561 gggcttatca tgattacacg accaagcttt cggtatctat cgtttatgcg ttgatcgcat
    37621 cagtcgcatt gaatttcttt tatcagccag gacatattta ttcaagtggt attactggtc
    37681 tagctcagat tttgacgact ttgtcaaaat cactgatagg attcgaagta ccggtatcaa
    37741 caactttgta tttactgaat atacctttgt ttgtattggc gtggttaaaa atcgggaaga
    37801 agtttacaat tttcactttt atcacagtca ctttgacctc cctttgtatc catattttac
    37861 cggaaacagt cctatctacc gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg
    37921 gactaggtat tgggctagcg ttgagaaacg gcctatcatc gggcggtcta gatatcgtga
    37981 gtatcacgat tcgtaaaaaa acaggaaagt cgattggttc tatttcgatc tacttcaatg
    38041 cattgatcgt ttttgttgcc ggaattctat ttggctggca atatatgttt tacagtacgt
    38101 tgtctatttt cgtcagtgga aaagtaactg atgtcgttta tactaaacaa aagaagatgc
    38161 aggtaatgat cgtaacgaaa catccagaaa aagtagtaaa tgaaatccaa aactgtatgc
    38221 gtagagggat cacgatcatc aatgaagcag aaggtgctta taagcatgat aagcagacag
    38281 tattgatgac gatcgttaca cgatacgaac tgcctctact tagagaagca atgaaagctt
    38341 ctgatccaca agcttttgta agtattgcag aaaacgtcca gatacttggt cgtttctatg
    38401 aggatggcat ttagatcgag tttgacaaaa attcatgaaa tactgctgta gacagacgtt
    38461 cgtttgtctg cagttttttt ctaccatagt agaatcagca aagaaaaact gcttttttat
    38521 ttgaaatttt cttaaagttg aaaaaaagca tgtttatttt tttaaattta tgggatgata
    38581 gaaaaaaata gtagagaaag gacacgattc tacttgctat tttcacttaa tacccgtata
    38641 ttacgaatga aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta
    38701 aaaaacagcc gctacatcac tgggtttgat gggctccgtt cattagctgt ggtgggtgtt
    38761 atcttctatc accttctgcc aactagtatg aaaggcggct atttgggcgt tccgattttt
    38821 ttcgtcgtat ctggttattt gatcacggat ctgctgcggc aggaatggga acaaaatgga
    38881 aaaatcaata tttggcaatt ttatgtcaga agaatgaaga gactctatcc ggcacttgtt
    38941 tttatgcttg tgacagcttc ggcttatatc actttgtttc agcgagggct gttaaacaat
    39001 cttcgaggag ttgtggtcag cagcttactg tatgtcaaca actggtggca aatcaataac
    39061 ggtttatctt atttcgatcg atttgctaat gagtcaccgt ttacgcatat ctggtcttta
    39121 gcagtagaag gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt gatggtattt
    39181 gttcgtaaaa gaaaatggat cttttatact gtgttaggta catccttcct ctccgccatc
    39241 ttgatgatga tcttgtatac accaggaggc gatccaaccc gtgtttatta cggtaccgac
    39301 actcgtctgt tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt
    39361 ttaaagaaaa acatcccgaa acaagcgaaa aatattctga atatcgctgg aggaatctcg
    39421 cttttggcac tgattctttg cttcttcttt ttagatgatc attacaattt catctattac
    39481 ggaggaatgc tcctagtgag tctgttatgc gtagtgctag ttgcagtgac agcacacccc
    39541 ggagcaagtt tgaatcgatg gctgacaaat ccagtattta cttggattgg caaacgaagc
    39601 tatggcattt atctgtatca gtttccagtt atgatttttt atgaagcaaa ggtgactgat
    39661 attgccgatc atgttttgtt gcatacttta gtggaaatca cattgattct aatcatcagt
    39721 gaactttctt atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc
    39781 caagtgatga aaggatggtt cagcaaaccg atcatcagta agagcaaacc atgggttgtt
    39841 ccaggcacat tacttgtagc aattgcactc gttggtttcg ttacggcacc gaaaaacgct
    39901 gtaacagccg atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa attagcagaa
    39961 gaaaccagaa acaattcaga aaacaccact agttcggttg accaagcgat ccttgataag
    40021 tacgagttgt ccaaggcaca agggaataag gcacaaaaat tagaactaac ggcatttggt
    40081 gattcagtta tgttggatgc tgcaacggat ctacaagaac tttatccaaa agtagtggta
    40141 gatggagatg tcggacgaca attatatgcc agtgtaccgt acattgaaaa attaaaggaa
    40201 caagatttgt tgaaagacac tgttctgatt ggactaggaa cgaatggctc attctcggaa
    40261 gctcagtttg atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc
    40321 cgagtaccga cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa
    40381 aaatacgaca atatgacgct gattgattgg tacagctaca gtaatgacca tagtgactgg
    40441 ttttatgatg atcaagtcca tccaaatcct gaagggatga cacattacat tcacttagtc
    40501 agcgaagcct tgctaggagg aagttcaagt acgaacagca gtgagacaaa cgtgacagaa
    40561 tgatagctac aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt tttttatatt
    40621 taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa
    40681 taacgttctt tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg
    40741 cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt ttgaagacga
    40801 gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag atcggttgta
    40861 tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg ttcatttgcc
    40921 atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag tgatcaatgg
    40981 tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta acaaaatatt
    41041 tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc
    41101 ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag taaagcgtcc
    41161 atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg tatgaatctc
    41221 atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca gccctttgaa
    41281 ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag ataagctgtc
    41341 tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg cgatactacg
    41401 tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg cctgggttcg
    41461 tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt
    41521 tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag aaaacacttt
    41581 ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg
    41641 aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta tttttattaa
    41701 aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac cataagaagg
    41761 gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta ctattagcct
    41821 tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat gagaattaca
    41881 tactagttgc tccccatcgt acttggtggg acccattata tctggcggta gctgcagcac
    41941 ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt ctgcgattta
    42001 tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca agcgtcatta
    42061 aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt cccagtggga
    42121 caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg tctaaagcga
    42181 aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc aagcgtcaga
    42241 gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa acggataaag
    42301 aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg gacaaagaag
    42361 tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc
    42421 ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac gagggagaga
    42481 cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa
    42541 tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa tgcgtttttt
    42601 acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga ttgaagacca
    42661 gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt
    42721 gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag aattatacaa
    42781 tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta tatcaggaaa
    42841 tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt cgaactattt
    42901 tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg tacaattttt
    42961 ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg acaatatccg
    43021 aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat ttgattcaga
    43081 aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat ctgattcgga
    43141 aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta caccgtttga
    43201 ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata atgctcgcta
    43261 tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag gcgtatggat
    43321 cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc tgcatgatat
    43381 tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc agtccgtcaa
    43441 tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga atatgatgaa
    43501 ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga
    43561 acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat tagcagaagc
    43621 attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat ggctcggaga
    43681 aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa aattacaact
    43741 taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga cagaagcacc
    43801 gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg atgggatcac
    43861 cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga tgcgttcttt
    43921 gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc agcagatgag
    43981 ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa
    44041 aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag aaataaaaca
    44101 aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa
    44161 acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt tggtttcatc
    44221 aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt tccaacagtt
    44281 caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc atttagagca
    44341 agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg
    44401 gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa aacaaatcac
    44461 ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag aaaattccta
    44521 ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa taaacaagca
    44581 aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga ttgggtatta
    44641 tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa aagttgaaaa
    44701 aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa
    44761 ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga
    44821 agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat ccttacgtag
    44881 taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat tgaatgccga
    44941 gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc aagaaaattt
    45001 actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa aatatctaga
    45061 tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat
    45121 aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc tattgaaaga
    45181 acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct
    45241 tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg aacaccccat
    45301 acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag aacaacaact
    45361 ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca
    45421 tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg caacgaatat
    45481 acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat ttcctgagat
    45541 aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag atagacgaaa
    45601 aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag cagaaaagct
    45661 cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc agctccagca
    45721 actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata gtaactgttt
    45781 agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc aactagctgt
    45841 tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga cacagcttga
    45901 tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat
    45961 agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc
    46021 acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc atgaagagca
    46081 attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg agacattccc
    46141 agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg aaataaagca
    46201 agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct acgatgaatg
    46261 tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat tacagcagct
    46321 ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt atgtgcttca
    46381 ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa
    46441 tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa ccggattaga
    46501 aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg
    46561 tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga tccagacaca
    46621 agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt cattagatga
    46681 agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag gaagaatgat
    46741 cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa taagaattga
    46801 tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag ggagattggt
    46861 aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga
    46921 aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg cagaagtctt
    46981 gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag attactgcca
    47041 atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg aactttattt
    47101 acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa
    47161 agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa
    47221 tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg agactaatca
    47281 gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat tggcatcacc
    47341 gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca cagaaaaaga
    47401 aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga
    47461 agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat tattaggtac
    47521 gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc cagtagaaca
    47581 agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc agataaatga
    47641 acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat tcaaagtgga
    47701 aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt tctttatgca
    47761 ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc aacaaacggt
    47821 ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag tgttcaagaa
    47881 aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga tcggcgacgt
    47941 agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat ttattttctt
    48001 tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg gtgaagtcat
    48061 ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg tgttacgtga
    48121 cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc agaaaaatga
    48181 aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt tccgcacaga
    48241 actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc gagagttgta
    48301 tttagatgcg cataaacatc aaccgaaaat cgaagtaacc gagaccggtt catggctcga
    48361 tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt
    48421 gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt ttgattcaga
    48481 agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac gtacagaaga
    48541 aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat tggaaaaaag
    48601 caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc atccagaaag
    48661 ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc aaaagcaagg
    48721 ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag ccgatgaaat
    48781 gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag aagaacggaa
    48841 aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc aggcagaggt
    48901 gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa aagaacgtga
    48961 agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct taagacagga
    49021 tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg cacaaatggt
    49081 caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac ctcagcggtt
    49141 tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac
    49201 gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg cagaagaaat
    49261 tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt tgaaagactt
    49321 gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa aaactgttta
    49381 tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg aagcattcaa
    49441 gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt gctgcgatcc
    49501 tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag taaaagatct
    49561 actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat ttacagggat
    49621 gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt atttaagagg
    49681 cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga
    49741 tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg gagcagatac
    49801 ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc
    49861 acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg aaggaacgat
    49921 tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca
    49981 aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga ttttaagtgt
    50041 aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa gaaagccttt
    50101 ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga ttccatttta
    50161 tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac aaagattgtc
    50221 ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt
    50281 tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact ttttgcacct
    50341 agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag catatgaaat
    50401 tctttctcag ccagctgaaa aatctctttt tctggttttt ggaaacctgt tgcttgagaa
    50461 atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag tttttttgtc
    50521 tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc cttcaagaaa
    50581 tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc ctcgtaaatt
    50641 ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc ttttagtgat
    50701 tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat tacttttggg
    50761 aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc tgcatctgaa
    50821 acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc atacatcgta
    50881 tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg gttttcattg
    50941 attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat
    51001 aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta tatttcagtt
    51061 tttttcacta aaaatcgtga aacacgtcaa taagtttgat gaacaaatta atcattatgg
    51121 tagaagtcga ttgatttatt ttaatgcaat ctctgtggaa ttttcgaaag atttattttt
    51181 ttaatacagt ctcaatataa ctattcaaac ataaaataac gttatttgat ttatttgttt
    51241 atagtaaatt ttatttagta atcattgctt taaaatacta cgct
//
LOCUS       DAZKNH010000008        49451 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000008 DAZKNH010000000
VERSION     DAZKNH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.008"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(234..1961)
                     /locus_tag="ACG8MG_000439"
     CDS             complement(234..1961)
                     /locus_tag="ACG8MG_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFQ0374517.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1954..3147)
                     /locus_tag="ACG8MG_000440"
     CDS             complement(1954..3147)
                     /locus_tag="ACG8MG_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0374518.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3334..3978)
                     /locus_tag="ACG8MG_000441"
     CDS             complement(3334..3978)
                     /locus_tag="ACG8MG_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0374519.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4072..4206)
                     /locus_tag="ACG8MG_000442"
     CDS             complement(4072..4206)
                     /locus_tag="ACG8MG_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFQ0374520.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4208..6340)
                     /gene="feoB"
                     /locus_tag="ACG8MG_000443"
     CDS             complement(4208..6340)
                     /gene="feoB"
                     /locus_tag="ACG8MG_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFQ0374521.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6337..6810)
                     /locus_tag="ACG8MG_000444"
     CDS             complement(6337..6810)
                     /locus_tag="ACG8MG_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFQ0374522.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7211..7435
                     /gene="nrdH"
                     /locus_tag="ACG8MG_000445"
     CDS             7211..7435
                     /gene="nrdH"
                     /locus_tag="ACG8MG_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFQ0374523.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7594..9753
                     /gene="nrdE"
                     /locus_tag="ACG8MG_000446"
     CDS             7594..9753
                     /gene="nrdE"
                     /locus_tag="ACG8MG_000446"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFQ0374524.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9803..10768
                     /gene="nrdF"
                     /locus_tag="ACG8MG_000447"
     CDS             9803..10768
                     /gene="nrdF"
                     /locus_tag="ACG8MG_000447"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFQ0374525.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10857..11996)
                     /locus_tag="ACG8MG_000448"
     CDS             complement(10857..11996)
                     /locus_tag="ACG8MG_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0374526.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12305..13018)
                     /locus_tag="ACG8MG_000449"
     CDS             complement(12305..13018)
                     /locus_tag="ACG8MG_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFQ0374527.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13272..13973)
                     /gene="nagB"
                     /locus_tag="ACG8MG_000450"
     CDS             complement(13272..13973)
                     /gene="nagB"
                     /locus_tag="ACG8MG_000450"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFQ0374528.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14207..15739
                     /locus_tag="ACG8MG_000451"
     CDS             14207..15739
                     /locus_tag="ACG8MG_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0374529.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15930..17132)
                     /locus_tag="ACG8MG_000452"
     CDS             complement(15930..17132)
                     /locus_tag="ACG8MG_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0374530.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17214..17903)
                     /locus_tag="ACG8MG_000453"
     CDS             complement(17214..17903)
                     /locus_tag="ACG8MG_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0374531.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18009..18377)
                     /gene="rplL"
                     /locus_tag="ACG8MG_000454"
     CDS             complement(18009..18377)
                     /gene="rplL"
                     /locus_tag="ACG8MG_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFQ0374532.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18430..18933)
                     /gene="rplJ"
                     /locus_tag="ACG8MG_000455"
     CDS             complement(18430..18933)
                     /gene="rplJ"
                     /locus_tag="ACG8MG_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFQ0374533.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19137..19264)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19343..20032)
                     /gene="rplA"
                     /locus_tag="ACG8MG_000456"
     CDS             complement(19343..20032)
                     /gene="rplA"
                     /locus_tag="ACG8MG_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFQ0374534.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20136..20558)
                     /gene="rplK"
                     /locus_tag="ACG8MG_000457"
     CDS             complement(20136..20558)
                     /gene="rplK"
                     /locus_tag="ACG8MG_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFQ0374535.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            20901..21569
                     /gene="sdaAB"
                     /locus_tag="ACG8MG_000458"
     CDS             20901..21569
                     /gene="sdaAB"
                     /locus_tag="ACG8MG_000458"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFQ0374536.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21582..22454
                     /gene="sdaAA"
                     /locus_tag="ACG8MG_000459"
     CDS             21582..22454
                     /gene="sdaAA"
                     /locus_tag="ACG8MG_000459"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFQ0374537.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22545..23645)
                     /locus_tag="ACG8MG_000460"
                     /pseudo
     CDS             complement(22545..23645)
                     /locus_tag="ACG8MG_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(23737..24624)
                     /locus_tag="ACG8MG_000461"
     CDS             complement(23737..24624)
                     /locus_tag="ACG8MG_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0374538.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(24701..25246)
                     /gene="nusG"
                     /locus_tag="ACG8MG_000462"
     CDS             complement(24701..25246)
                     /gene="nusG"
                     /locus_tag="ACG8MG_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFQ0374539.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25347..25517)
                     /gene="secE"
                     /locus_tag="ACG8MG_000463"
     CDS             complement(25347..25517)
                     /gene="secE"
                     /locus_tag="ACG8MG_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFQ0374540.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25541..25693)
                     /gene="rpmG"
                     /locus_tag="ACG8MG_000464"
     CDS             complement(25541..25693)
                     /gene="rpmG"
                     /locus_tag="ACG8MG_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0374541.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            25855..26499
                     /gene="cbpA"
                     /locus_tag="ACG8MG_000465"
     CDS             25855..26499
                     /gene="cbpA"
                     /locus_tag="ACG8MG_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFQ0374542.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26555..27058)
                     /locus_tag="ACG8MG_000466"
     CDS             complement(26555..27058)
                     /locus_tag="ACG8MG_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0374543.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27071..27117)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            27655..28620
                     /locus_tag="ACG8MG_000467"
     CDS             27655..28620
                     /locus_tag="ACG8MG_000467"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0374544.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(28719..29891)
                     /locus_tag="ACG8MG_000468"
     CDS             complement(28719..29891)
                     /locus_tag="ACG8MG_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0374545.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(29878..31023)
                     /locus_tag="ACG8MG_000469"
     CDS             complement(29878..31023)
                     /locus_tag="ACG8MG_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0374546.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31037..31961)
                     /locus_tag="ACG8MG_000470"
                     /pseudo
     CDS             complement(31037..31961)
                     /locus_tag="ACG8MG_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32078..33157
                     /locus_tag="ACG8MG_000471"
     CDS             32078..33157
                     /locus_tag="ACG8MG_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0374547.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33154..33782
                     /locus_tag="ACG8MG_000472"
                     /pseudo
     CDS             33154..33782
                     /locus_tag="ACG8MG_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(33892..34119)
                     /locus_tag="ACG8MG_000473"
     CDS             complement(33892..34119)
                     /locus_tag="ACG8MG_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0374548.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34222..36810)
                     /locus_tag="ACG8MG_000474"
     CDS             complement(34222..36810)
                     /locus_tag="ACG8MG_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFQ0374549.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(36830..37489)
                     /locus_tag="ACG8MG_000475"
     CDS             complement(36830..37489)
                     /locus_tag="ACG8MG_000475"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0374550.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(37538..38041)
                     /gene="trmL"
                     /locus_tag="ACG8MG_000476"
     CDS             complement(37538..38041)
                     /gene="trmL"
                     /locus_tag="ACG8MG_000476"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFQ0374551.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38262..39128)
                     /locus_tag="ACG8MG_000477"
     CDS             complement(38262..39128)
                     /locus_tag="ACG8MG_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0374552.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39237..39878
                     /locus_tag="ACG8MG_000478"
     CDS             39237..39878
                     /locus_tag="ACG8MG_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFQ0374553.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(39940..41301)
                     /gene="mgtE"
                     /locus_tag="ACG8MG_000479"
     CDS             complement(39940..41301)
                     /gene="mgtE"
                     /locus_tag="ACG8MG_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFQ0374554.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41319..42218)
                     /locus_tag="ACG8MG_000480"
     CDS             complement(41319..42218)
                     /locus_tag="ACG8MG_000480"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0374555.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42220..43017)
                     /locus_tag="ACG8MG_000481"
     CDS             complement(42220..43017)
                     /locus_tag="ACG8MG_000481"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFQ0374556.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(42995..43699)
                     /locus_tag="ACG8MG_000482"
     CDS             complement(42995..43699)
                     /locus_tag="ACG8MG_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFQ0374557.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            43830..44406
                     /locus_tag="ACG8MG_000483"
                     /pseudo
     CDS             43830..44406
                     /locus_tag="ACG8MG_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            44524..45201
                     /locus_tag="ACG8MG_000484"
     CDS             44524..45201
                     /locus_tag="ACG8MG_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFQ0374558.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45280..47088)
                     /gene="pepF"
                     /locus_tag="ACG8MG_000485"
     CDS             complement(45280..47088)
                     /gene="pepF"
                     /locus_tag="ACG8MG_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFQ0374559.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47264..48262)
                     /locus_tag="ACG8MG_000486"
     CDS             complement(47264..48262)
                     /locus_tag="ACG8MG_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFQ0374560.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(48488..49141)
                     /locus_tag="ACG8MG_000487"
     CDS             complement(48488..49141)
                     /locus_tag="ACG8MG_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFQ0374561.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14784 a        10395 c         8719 g        15553 t
ORIGIN      
        1 aatgcacttc gtacataata aactttaatc acattagtct ttcataccta cgatcgatct
       61 ctttgactcg cacccataaa ttcataatag tttgtcggcg tctttggacg tttcgaagat
      121 gagctacgca ttctcttttt ttatagacaa aaaagataga gacgaacatg gagcttccaa
      181 atttggatat ttgatccaat tttggaagct ccatttattt cgtgtctgtc tttttatttt
      241 ttccagcttt cgactaattc ttggttctct ttgatccagt cttttgctgc ttgctgtgga
      301 tcttttccat tgttgatttc caacataact ttttccatat ctttttctga ccaatggaaa
      361 ttgtctaata ctttgtaagc ttctggctgg tcttctttca atccttttcg aaccatcgta
      421 tggatctgct cttcgcttcc cattccgttt tcaggatctt ctaagtattt cagatcatat
      481 ttggcaaaca tccaatgagg tgtccatcct gtaactacaa ttggttcgtg ttttttgatc
      541 gcttgaccta gtgcaacggt cattgctcct gaagaagatg tctctacttt ccagtctttc
      601 aagttgtcat atttttggat cgtgttttca gcagccgtaa ctacaccagc tcctggctcg
      661 attcctatga ttttcttacc tgcctgatcg gttaactcat cgatcgaatt tacatccatg
      721 taggctggca ctgctaaacc aacttttgct cctgtcaaat tggcacctag gtcttcgact
      781 tgatctttat attgtgcata ttgtgaacca tgagttttag gtagccaagc tgaaaccatg
      841 gcatctgact cgccatttga aacagatttc cacatgattg aattatctaa tggcgtcatc
      901 gttacatcat agcccatttg tttcaatact tcaccgacta catttgttga agcaacttct
      961 gtatcccatt caacataaga taagttgata cgttttgttt ctttggcaga tgaaaaactc
     1021 cctataccaa tcgcacctaa gatcactact actacagcac caatgatcag tccttgacgt
     1081 tttttacttt tctgtgttgt tgctcctgca gcttttttat tgtttggctg gttcaaatgc
     1141 tgtgtaaaac ggtcaatgat gattgctaag ataactaatg caaccccgtt gacaaatcca
     1201 ttcccgactt gtgctctttg caatgctgat aatacgccgc gtcctaagcc gggtgcaccg
     1261 atcatagagg caataacgac cattgaaagt gacagcattg ttgtttgatt gatccctgcc
     1321 atgatcgtac ttttagctaa aggcaattct agtttgaata gtttttgacg acttgtgctc
     1381 ccgaatgaat cagaagcttc tactaattct ttaggaactt gtcgaatccc taaattcgta
     1441 aaacgaacgg ttggtggcag agcaaagatg actgaggcaa atacccctgg aaccatcccg
     1501 ataccgaaaa atgcaactgc tggaatcaaa taaacgaatc ctggcattgt ctgcataaag
     1561 tcaaggatcg gtttgataat actttgggct ttactgcttt ttgccatcaa gatccctaat
     1621 ggtaccccaa tgatgatcga gatcacacta gataaaagaa caagtgtgac agtattcatc
     1681 aaatcattcc atagaccttg gttataaata aaccatagac cgattaatgt gaacaacgta
     1741 agtcctggac gtttcttaga aataaagaaa gctgctaccg tcaataacaa gataaacaaa
     1801 ggagctggaa tcactaacag taaatttgta atccccgaca tcactgcctg tccaatcgtt
     1861 tgtaaaaatg aaaataggcc agaaaaagtt gtcgtaaacc attctgtgat attttctacc
     1921 cagtttgcca ctggtaattg atagttcaat agattattca tgttcactca cctcgcttgt
     1981 ttctgctaat gcttcaatca cactacctcg gatgacgaca ccttttaatt ttccatcctc
     2041 tacaactgca agtggagtcg gtgagtcata aataatattg aaaatatcac ttaccaccat
     2101 gtctttccca atcaccgtca cattttcatc taggaagtcg atcaatggac gtttttgctt
     2161 cctagcttct aacgcttgat ctgcagtgat gatccctttc aggtgtctct ttctgtcaac
     2221 agccatcagc atgctgactt cttcattacg catccttgtc aatgcaacgt ttggtccatc
     2281 agattcgata ttcgtcgtca atgcaggtac catgatgttt tgagccgtaa gcactttgga
     2341 acgatcgact tcttccacaa attcacgtac ataatcattt gctggattgg tcaatatttc
     2401 ttcacctgtt ccgatctgca tgatttcgcc gtctttcatc aaggcgattc tgtccccaat
     2461 ccgtaaagct tcattcaaat catgggtaat gaaaataatc gttttttgaa cattggcttg
     2521 caggtccagt aattcgtctt gcatttctcg ccggatcagc ggatcaagag ccgagaacgc
     2581 ttcgtccatc agcaagatct caggatcatt tgctaaagct ctggctaggc caacccgttg
     2641 ttgcatccca ccagataact gactaggata ttggtcttta aatgaaagca aactagaatt
     2701 ttctaatgct ttttctgctt tttcttgtcg ttcttcttta ggtacaccac ggacttctaa
     2761 cccgtattct gtattttcca agatcgttcg atgtggaaac agaccgaagt tttggaaaac
     2821 catattgatt ttatgacgac ggacttctcg caattcttct ttgcttaact tcgatacatc
     2881 ttctccatca atatagatac tgcccgaagt tggctcaatc agacgattca atagacggat
     2941 caatgtggat ttcccactcc cggaaagccc catgatcacg aagatttctc cttcttttac
     3001 gtcaaaattc acgtcataca caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt
     3061 cggctggtga tcattcatca tatctaaagc ggcttgtgtc tttttaccaa atatctttgt
     3121 taaatgtttt acttctactt ttaccaaaca aattctcctt ttttatgtaa attcattttt
     3181 ctttctgaaa aaagtgacca cactatgtta acatcatgta gtcactttgt caaaaatgaa
     3241 agaaatttgc ttgtccaaac tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta
     3301 gccaagttaa tattttttta atatttcaca caatcattct gcaagctgat gctccgggta
     3361 aaaaaagttt gtcatacgct gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc
     3421 tgccgtaaat ttagcatagg ggccaggtga aacaagcagt tcttcattat gagaaattgc
     3481 gataactgtg gcgccagtcg cctgccagat attcaactcg ctaattgaac ggtccagatg
     3541 cattgctttt tctgataaag ataattcaaa cggaagtaaa ggattcatct tatgtattcg
     3601 cttcgtttga ttgaccagct gatccaataa ttgagaaaaa tgattcagct catcttgttg
     3661 cttctccaca cttgccatga tttctgcttt tgtatcttgg atggattgta catcttgata
     3721 ttgcaatagg aaatcttttg ctttttcctt cgaagcaaca aatgccccac ttccatgccg
     3781 tacttccatg attccaagat ctaccagcac attgatagcc ttgcgggtag tctcaggaga
     3841 agcattgtac gtactggcaa gtgttgagcg ggcatggatc ttctcaccca ctttatatcg
     3901 gttttcaacg atacgttcgg ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat
     3961 ttttttcttt ccagacattt tctttcctct ttcttttttt actttccctc atcttactat
     4021 gctctagcaa aataagcaaa aaaggggaga atagcctctt gcaacgactg atcatcgctt
     4081 gatctgttcc ttttttactg ggcaagctgt ttgacagtca tcacaattct taccggattt
     4141 taaacgagta tagacaattg cgcctgcact tccaaagatt aacacactta ataagaatgt
     4201 cgccatccta ttcctctcct tctaatgaca atatagtata aaactggttc ttggattttg
     4261 gctgacggaa caaaacataa acgagtccta tcatcagtaa aatagcaaga tacgtcccta
     4321 acgctacctg tccaccctcc aataagtgtc ccaactgata aatcacaaaa ctaactaaat
     4381 aagctaaacc gcattgatag cctatggcga tccaagtcca tttcttggca ttcatttccc
     4441 gatggatagc cccaattgcc gcaaaacaag gcgcacaaag taaattgaag gctaacaatg
     4501 aataagctgc tgcgggagta tatgtgtgtt gtagcacttg ccagatttct ctgccatttt
     4561 cggatacttc cgcatgacca aacaaaatac caaatgtgcc aataatattt tctttagcga
     4621 ccaatcctgt aatcgctgcg actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa
     4681 caactgccag gattcgcccg aaaaaggcta agatactttg gtcttcagaa acaggttgag
     4741 cataaaaatt ataagaagag ctgaaccaga tcacgatact agaaacaaag ataatcgttc
     4801 ctgctttttt aacaaaagac ttgctgcggt cataggtttg tcttaggacc gctgaccatt
     4861 gaggaaaatg atatgcgggc aattccatga taaaaggagc tgtatctcca gcaaacaaac
     4921 gtgttttttt caagctgatt ccagagaaaa taattgcagt cataccaaga aaataggcag
     4981 aaggagctac ccaagattga tgaggaaaaa aggcccctgc tactaaggca ataatcggca
     5041 actttgcaga acaaggcatg aaggttgtga ccatgatcgt caaacgacga tcttgctcgt
     5101 tttcaatcgt gcgactggcc atgacgcctg gcactccaca tcctgtagca atcagcatag
     5161 gaatgaagga ttttcctgaa agaccgaatt tcctgaaaag tcgatccatg acaaaagcga
     5221 tcctcgccat atatccgcag tcttccagaa agctcaagca taaaaacaat ataatcaatt
     5281 gcggtagaaa tcctaacact gctcccacgc ctgcgatgat gccgttcaag atcagctcct
     5341 gcatccatcc cgctacttgc cattcagcta acaatctgcc aacatgttct ggtaaccatg
     5401 taccaaacaa tgtgtcattg atccaatctg tccccattgt tcctactgtt tgaatagcca
     5461 gataatagat cagccacatc acaaatgcga agatcggcaa ggctaaccaa cgattcgttg
     5521 caatctgatc gattttgtca ctcatcgata attggaaaga atcatttgac acaacacaga
     5581 gtgtacatag ttgggtaata aattcataac gttcattgac cagaatcgtc tcactatcgt
     5641 cgtccaatag tttctctgtt aactggatca gttgctcgat ctctttttgc tgagaaactg
     5701 ctatctcgta atcctctctt accttctgat ctcgttcaaa atatttcatt gcataccagc
     5761 gtttcttgcc gctttgttct cctagtacat cttcaatttc atgtaaagct gcttctaaac
     5821 gtggatcata tgtacaggga acaggaatag aaggccgctt ttttgcagac tcctgaatct
     5881 tttcgattag tttttcgatt ccttgctttt tcaatacgct gattggtaaa actggtacac
     5941 caagaccata ggaaagcttt tctagattga ttttttttcc ttcttttttg atgacatcca
     6001 tcatattcag accaatgatc ataggaatgt ctaaatccaa taactgcgta gttaaataaa
     6061 gatttcgttc taaattggta gcatcaataa tgttgatgat cagttcaggc tgttcctgaa
     6121 ccaaataatc tcttgcaatc acttcttctg gtgtataagg agacaaagaa tagatgccgg
     6181 gtaggtcctg aatatgaatg ttcggatttt ttttcaacga tccttctttt ttttctactg
     6241 ttacgcccgg ccaattccct acatattgat tcgaaccagt caagagattg aacaagctag
     6301 ttttcccgct gtttggattt ccgactaacg cgatattcat tctttttcct ccctttgtat
     6361 caagattagt tcggcctctg actttcgtaa cgtcagttca taccctcgca ctgtcagttc
     6421 gattggatct cccagagggg ctagttttcg gataactatt tttacatttc gcgtcaatcc
     6481 catatccatt aaccgtcgct tcactgcgcc ttgtccgtgg attgctacga cgatcccagt
     6541 ctctcctgct tttaattgat caagagaagt ccagctttca gaggaagcag tctttttatt
     6601 gacaaagatt tgacctagta tttcccgact tactgccact cgatgacttt ttaccagtaa
     6661 aatggcattg ttgcctgata aattgacgac gacgatttta cttccggcaa cgatcccgat
     6721 attttgcaaa tggcgttttg ttttttcttc tgtttcgatt cgttcgactt gataaacgaa
     6781 attctgcttt gcctgcgcta aggtctgcat attgattcct ctcttgtgaa ataaatcatg
     6841 tattaaaatc attcattata tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat
     6901 cattctcaca tagaatactc tcggtaattg agaatgtcaa ttatacttgt gatattatga
     6961 gcgaatcttt agtattgata gataaaaatg agaaaaaaat atttcttcat agttttccac
     7021 aattttttct ctattaaatt tgctattttc ccacacgata cttgttttca gctctttttt
     7081 caaaaacaca aaacttagta ttccacagtt tccacaacac aatatgtagt gtttgttgtt
     7141 tgactttttt actaagagtt ttatactatc tattatagat aataagaatg tgttagagga
     7201 ggcactcacc atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt
     7261 caaacggttt ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc
     7321 tgaagcagta gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga
     7381 tgcagaaaca gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga
     7441 ctaaaagaat caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt
     7501 cgcaaacttc caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca
     7561 tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc
     7621 agttatttca agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat
     7681 aaggataaag aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact
     7741 tttctatcta ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa
     7801 ttcattcaat tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa
     7861 catttttctt tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa
     7921 accaacgacg gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt
     7981 tactttgcga acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa
     8041 cgatatcagc cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg
     8101 gtgtcttgtt tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac
     8161 tctgctttgc aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt
     8221 gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg
     8281 aaattattcg aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc
     8341 gtggtttatt tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa
     8401 aatgcagatg aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa
     8461 ttttatgaat tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa
     8521 aaagaatatg gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt
     8581 gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca
     8641 aaattacaac aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag
     8701 aatccaatcg atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa
     8761 acgccttctg tgattaacgg caaacaggaa tatgaagtat taggaacgga tatctcatgt
     8821 aacttaggct cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg
     8881 cgtacgatga ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca
     8941 ccgatccaaa acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac
     9001 acttatcttg caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat
     9061 atttacttca tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa
     9121 agaaaacaag ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa
     9181 aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc
     9241 atttttatcc caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga
     9301 ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat
     9361 acgagtgcaa gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc
     9421 ggtaaaattt attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct
     9481 gcttatgata tggacatgcg taaagtaatc gatgtttatg caatggcaca acaacacgtc
     9541 gatcaaggca tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa
     9601 tggaagacaa caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc
     9661 cacaaaggaa tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc
     9721 ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata
     9781 ttttaattaa aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga
     9841 aattgaagat atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga
     9901 tacacgtatc ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa
     9961 agatacagta ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag
    10021 tggcatggat cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa
    10081 tattcaattc atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa
    10141 tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa
    10201 aaaagctgag cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc
    10261 cagcgtcttt ttagaaacat tcttgttcta ttcaggtttt tatactcctc tttattattt
    10321 aggaaacaac aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc
    10381 tgtacatgga acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc
    10441 agaacaagaa acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga
    10501 agaacgttat acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt
    10561 cctacgctat aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga
    10621 tactgccaat gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca
    10681 tgacttcttc tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga
    10741 cgacgattat ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa
    10801 ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat
    10861 tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt
    10921 ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc atagacaggt
    10981 atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg
    11041 atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca
    11101 tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata
    11161 aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata
    11221 ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt
    11281 gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca
    11341 gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga
    11401 aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata
    11461 aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca
    11521 cttgcagata aactgactaa tatgatattg aacaaattac taagagataa atttgctgat
    11581 gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac
    11641 catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc
    11701 attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac
    11761 gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga
    11821 tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaactgc cgaaagcatt
    11881 ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg
    11941 agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat
    12001 ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga
    12061 ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact
    12121 taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat
    12181 aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga
    12241 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
    12301 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
    12361 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
    12421 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
    12481 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
    12541 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
    12601 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
    12661 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
    12721 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
    12781 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
    12841 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
    12901 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
    12961 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
    13021 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
    13081 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa
    13141 cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat
    13201 ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct
    13261 actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg
    13321 tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg
    13381 attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt
    13441 gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt
    13501 ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa
    13561 gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg
    13621 attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct
    13681 tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg
    13741 aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca
    13801 gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt
    13861 gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt
    13921 tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt
    13981 gctcctttat attttggtat agcccaatta tagcattctt tttttctttt tacaataatt
    14041 agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg
    14101 tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac
    14161 tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac
    14221 attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca
    14281 attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt
    14341 gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa
    14401 tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga
    14461 atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta
    14521 ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac
    14581 ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg
    14641 gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg
    14701 ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga
    14761 accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt
    14821 tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca
    14881 tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt
    14941 gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca
    15001 aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc
    15061 aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag
    15121 taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac
    15181 tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt
    15241 tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac
    15301 actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat
    15361 caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt
    15421 aagcagtcaa tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg
    15481 gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa
    15541 ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac
    15601 gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg
    15661 aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat
    15721 tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa
    15781 gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta
    15841 gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg
    15901 cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct
    15961 ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt
    16021 acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct
    16081 ccaataaagt acataaacat aaatacagta ttatttcggt tgcttgctgc ttcacctagc
    16141 ccctgtactc gtgtttgatt ggcgacttga ccaaattgat tcccgacgtc taataaaata
    16201 atagctaaaa gcaaaatggc caaatggctt ccgccaacca ataaagcaat aaagcttatt
    16261 gtttgcataa caagtcccat atacacgatt tttctttcag aataagaatc tgccagtcgt
    16321 cccacgatag gtgcagcaac tgctccagac aaaccaaaaa tcgctaatat ccctgcttcg
    16381 aatgttcccc aattgtaggc gggactgctg atataaaaaa tcaacgtaga ccaaaagatc
    16441 gaaaatgttc caaacatgaa aaatccactg actgccgctt ctcttaatag acgttgagaa
    16501 gcaactaaat gcggcaaact tttaagtgaa tcaaggtatg aaacagctgt actactttgc
    16561 tggacagaac gcggcatttt ccagtaaagc agaagtgtaa gaattccgac tgcacataaa
    16621 gcaaataaat agatcatccg ccaagaaaaa agactggcaa caataccaga taccgtccga
    16681 gaaagcaaaa tccccacgag taaaccgcta agcaatgtac ccatgacctt ccctcttgct
    16741 tttggtcccg ctagtaccgc accgtaagga atgatgattt gcggcacgat cgaaagtaat
    16801 cctattaaaa aagaagcgca tgcaaataac gtaaacgaag gcgcaaaaaa agcggccaac
    16861 agagagacgg cagataacgc tgccattcga atgatcaatt ttggacgatc caccacgtcc
    16921 cctaaaggaa ctaaaaacag taaccctaac gcatagccta attgggtcaa catcgtcaat
    16981 atcccgactg cacttgtgct aacagataaa ctagctgcta cttttgttcc aatcggctgg
    17041 atgtaataca tattggcaac aacaacgcca caagtgattg ataatagtaa aataagcgaa
    17101 gttgttaaag gttttttttc attactcttc attgatttcc cccatttcct atcttagttt
    17161 acaacttatg gaaaagattg gaaatagaaa aatacaacta ttcgccacac ttattatttg
    17221 tgcttttgtt ttccttgttt tttcttgcga tctttataaa tgaagacaac taaccctact
    17281 agcacagtaa cacttgcaac caacgtgact gcagttttgt tatatccgtg aaacagagct
    17341 tctccaccac acgcatacaa cacagacgaa gggatgactc caatcacgat cgacaatatg
    17401 atttgctgta gaggcaattt catcgcatct gcagcaaaat tgaccataga agatggaata
    17461 accggtatca tatagcctaa cattacacct atttttggat gtttcatttg tcggattgct
    17521 tgcacccaat gattcgtttc ggttttctta tctaaaaatt ttaagcgatg cataaggaaa
    17581 attgcagtga tgttaccaag agcattaccc aaaatgttga tgatcgcacc tgtcagtggt
    17641 ccaaagctca aaccagcaat gactccaact actgaagtag gaatacctgg aacagcacac
    17701 atcaccgctg tcaatacgat caagagtatc gatgcaaaag tcccatgcga acgaatatgt
    17761 gcgagtagct ctgccttact cgctctcgga tttaaaaata attggatgtc tgtctgatat
    17821 tctaaataca accgatagcc cactaccagt atcagaatga ttcccaacga gatcaatatc
    17881 aatcggatta ttttcttttt catatttcct cctgaaaaaa atctatattc attaaataat
    17941 agtttaacat aaaaacaaaa aagcaccgaa atccaacata ggatctcagt gcttttaaat
    18001 agtaaaaatt atttaacagt tacagatgcg ccaacttctt caagtttagc tttcaattct
    18061 tcagcttctt ctttagatac gccttcttta actggtgcag gagcgccatc aactacagct
    18121 ttagcttctt tcaagcctaa accagttgct tcacgaactg ctttgataac tttaactttt
    18181 tggtctccag ctgcagttaa ttctacagtg aattctgttt gttcttcagc agcagcagcg
    18241 ccaccagcag cagctacagc tacaggagca gcagcagata cgccaaattc ttcttcaata
    18301 gctttaacta ggtcgttaag ttctaggata gttgcttctt ttaactcagc aacgatgttt
    18361 tcaatgttca atgccattat aatttcctcc gtttaattgt tcttttttat ttaatgcttt
    18421 atgcggcaat taagctgcat ctccatcttc tttgtctgcc actgctttga cagcgtaagc
    18481 cacgttgcgg actggcgctt gtagtacaga tagcaacata gatagaagtc cttcgcgatt
    18541 tggcaatttt gccaatgctg tgatttcttc caatgaagag actttacctt cgatgatacc
    18601 gcctttgatt tctaatgctt ttgcttcttt agcaaattcg tccatgattt tcgctggtgc
    18661 gactacatct tcgttactga atgcaacagc tgtaggtcca gtgaatactt cgtccatgcc
    18721 ttcaaggcca gcttttttag ctgcacgtga aagaatagaa tttttgataa ctttcatttc
    18781 tacgttagca tcacgaagtt gtttacgtaa acgtgttact tcatcaactg ttaatccgcg
    18841 gtagtcaacg actactactg atgcagctgc tgtaaatttt tcgcttactt catcaacgat
    18901 tgctgctttt tttgcgataa ttgcttcact catttttgag tttcacctcc tgaattttga
    18961 tggaagagtt ttttgcactg aaaaggcaag tcacttttat agttactttg ctcaaccagt
    19021 attattcatc acctattcac ttcgttcata gtgattctac tcgctttgct cgaaaggaaa
    19081 ctcctttcgc tttgctcaac cagtattgtt catcatctct tcacttcgtt catagtgatt
    19141 cacaataaaa aactctatgc caccgcagac atagagggac tattgaatat attcaataaa
    19201 cgtcctcggt aggaaattaa ggcttgatgc cacctactgt cttcggtaca agttaattat
    19261 taacctcaac tagcttagca tgctaaacta gttgagtcaa gaattatttt tcagttggat
    19321 caatcaacat tctaatattt aattagaaag aagcttggtc aacatggata ccaggtccaa
    19381 atgttgttgt gatcgttaca tttttcatgt attgaccttt agctgctgat ggtttagctt
    19441 ttagcaatac atcatggatt gctgcaaagt tttcaactaa tttttcatcg tcaaatgaaa
    19501 cttttccgat tggtacatgg atatttccag ctttgtccac acggtaagtt actttaccag
    19561 ctttcacttc gtttactgct tttgttacgt ccattgtaac tgtaccagtt ttagggtttg
    19621 gcattaagcc ttttggtcct aatactcgtc ctagacgacc tacagtagcc atcatatcag
    19681 gtgttgcaac aactacatca aagtcaaacc atccgttttg gattttttga accatgtcgt
    19741 cgtcacctac gaaatctgca ccagctgctt cagcttcttt tgctttgtct cctttagcaa
    19801 aaactaaaac agtttgtgtt ttaccagttc cgtttggtaa tacaacagca ccacggattt
    19861 gttggtctgc ttttttaggg tctacgttca aacggtatgc aacttcaact gttgcatcaa
    19921 attttgcaat gtttgtttct tttgctaaag ctactgcttc ggcaacagga taaactttac
    19981 ttgaatcaac tttttttaat gcatcttgca tttttttgct ctttttagcc attttggttc
    20041 ctccttgatt gtggttataa cggttgtacc tcccacgtgt tctatatctt tcaatataga
    20101 gccaactgag aagctgctgt cttagggtgt atagattatt ctacagtgat ccccatgctt
    20161 cgtgcagttc cttcaaccat gcgcattgct gcttcaacgt tagctgcgtt taggtcttcc
    20221 atttttaatt cagcgatttc tcttacttga tcgctagaaa ctttcgcaac tttatttttg
    20281 tttggttcgc ctgaaccttt ttcgatttta gctgcttttt tcaatagaac agcagctggc
    20341 ggtgtttttg taacgaatgt gaatgaacgg tcttcgtata cagaaataac aaccggaatg
    20401 atcaaacctg cttgatcagc tgtacgagcg ttgaattctt ttgtgaatcc catgatattg
    20461 atacccgctt gacctagtgc aggacctact ggtggagctg gagttgcttt acctgcagga
    20521 atttgcaatt taacgatttt ttctactttc tttgccacga gacatacctc cttaagtccg
    20581 tgatgtggtt aattggagat gcatatttct cctcccacgt tttatctgat ctgaaaaaat
    20641 ccagacaaat aatgtgcaaa acaatgcaca ctggtacagt atagcaaatt tatttgtttt
    20701 ttcaagattt ttttctgcta tttttacatt ttgcttgaat aaacgctaaa tttattctaa
    20761 aaaaacaaca atttttcgtt ctcatttctt cttttatgtt acacttgcgc tagcatggtt
    20821 caacagatga aagcaaacaa taacacggtt ttttagaccc ttgtacaaag attcctacat
    20881 aacaaaatga ggtggagaaa atgaaagatt taaggtatag aagtgtcttt gatattattg
    20941 gacctgtgat gattggtcca agtagctctc acactgccgg agctgctaga atcgggaaaa
    21001 tcatacgtag tatatttggc gaacagcctg attctgtaga tatttatcta tatgaatcat
    21061 ttgcaaaaac ctatcggggg cacggaacag atattgcatt agttggcggc ctgttaggga
    21121 tggaacccga tgatgaacgt ttagctgatt ctttgaaatt ggcatatgaa gccggtatgg
    21181 aagtcttgtt tgttcctaag agtgaaaaag ccgaacatcc aaactctgta aagatgcttc
    21241 taaaaaaagg aacacgaaag ctttcagtca ctggcatttc gatcggcggt ggcaatatcc
    21301 agatttctga attgaatgga ttcaaaattt ctttaagcat gggtactcct accttgatcg
    21361 ttgtccacca agatgttcca ggtatgattg ctaaagtgac tagtatactt tctgagacca
    21421 atacgaatat cggcactatg acagtgacac gggaatcaaa aggtgaaaat gccattatga
    21481 taattgaagt agatgatcct caagtagaag aaacggtaaa aagactcaaa cagctgccaa
    21541 atatcgacag cgtaaattat ttcgattaag gagctaaaaa aatgttttta tcgattgaag
    21601 aattagtaga tcaagcaaaa gcttatccaa gtgtagcaga attgatgatt gcagttgaaa
    21661 tggaaatgag tggccgttcc cgtagtcaca tcatcgagat catggaaaaa aacctctcag
    21721 taatggaaca atcaatcaac gagggaaaag aaggtgttac ctccgtcaca ggaattaccg
    21781 gtggtgatgc aaaaaaatta gatgattatt taaatgaggc agacttttta agtggagaaa
    21841 caatcttaaa agctgtacgc aatgctgtag ctgttaatga agtcaatgca aaaatggggt
    21901 tgatttgtgc tacaccgact gccggaagcg cgggtgttgt tcctggtgtg ctgatggctg
    21961 tccgagatcg gcttgatttg acacatgaac agcaattgga attcttattt accgctggcg
    22021 ctttcgggtt agtcatcgca aacaacgctt ctatcagtgg tgcagaaggg ggatgtcaag
    22081 cagaagttgg ttctgcaagt gcaatggcag cagcagcttt ggtttgtgca aaaaatggga
    22141 ctcctcatca agcagcacaa gcagtagcca ttacattgaa aaatatgatg ggactgattt
    22201 gcgatcccgt tgcaggctta gttgaagtcc cgtgtgtcaa acgtaatgct ttaggctctt
    22261 ctcaagcttt tatctctgct gatatggcat tagcgggtat cgaaagtgtc attccgccgg
    22321 atgaagtcgt tgctgcaatg taccaagtcg gacgtcaaat gccgtcgatt ttcaaagaaa
    22381 cagcagaagg tggcttagct gttacgccaa ctgctaaacg acttgcggaa gagattttaa
    22441 ataagagggc gtgataaagc tccttaattt cgagtattaa gagattagtt atcttcataa
    22501 caaaaaacga agagaggact aattgccctt ctcttcgttt tttattaatc catcaatttc
    22561 gtatatttta cttcatcgat cgtttttgtt cctgcaagtt gcatcgtgat cgacaattct
    22621 ttgttcaggt gttcaaaaac agatgttact ccttctgccc cacctagatt caacccataa
    22681 atcactggtc ggccaatcgc aactaagtca gcaccacttg ccagagcttt gaagacatgc
    22741 tctcctcgac ggacaccaga gtcgaaaatc actggtacac gtttgttgac tacttcggca
    22801 atttttggca atacttcaaa ggacgcagga cctccatcta gttgacgtcc accatgattg
    22861 gatacccaga taccatctgc ccctgcagag atagcgatta ctgcatcttc tggcgattgt
    22921 attcctttta cgataactgg aagatgggtg atttctttga ttgttttgat gtcatctggt
    22981 gtcaagcctt gttttgcagc tgcataaatt tctgcgattc cttttccttc gccattacca
    23041 cttgcacttt gttcgctata cgcagctaag ttaggcattg gtaatggaaa ttggaattga
    23101 ttgatgacgt cttcttcacg gtaaccaccg agcgttgaat ctgccgttaa aatgatagct
    23161 ttagcccctg cttctactgc tttgttcaaa atgaactcat tgaatctgtc gtctttactc
    23221 atatataatt ggaaaaactg cggtgcgcct ggcgctgcat cggctgcgtc tttgattgtc
    23281 gtatttgcat aagtgctgat ggaaaagatt gatccagcag cagctactcc tttagctgta
    23341 tcagcttccc ctttttcatg tgccaatcct tgggccgctg atggcgcttg gataatcggt
    23401 gtatctaatt caattccgaa aacagatgta tgcaaatcgg ctgagtcgat tccgcgtaat
    23461 atccgtggca tgattttctt tgtattaaaa gaagtcgtat tttctttcat cgtccactca
    23521 tcttcagaac caccgcgaat atagccaaaa gctcctgctt ccatgcgctc ttttacacga
    23581 ccttctagtt aggcaatgtt tacaatatcg attggatgtt cttcattact tgtatgataa
    23641 gtcatagtag atccttcttt catcttcttg attacatata aatcatacta aacttacaaa
    23701 aagtaagcaa acatttactc tcagatactt ttatcgctat tctttccaaa taaatcgact
    23761 tacaagctga tctacttctt ggttttcatg aagcatacta tgagaggcat tttcacctgt
    23821 cactactttt tcctctattt catttccgcg ttgcttcaac aacgcataga ccgctaatgc
    23881 actgtttaac ggtactgttc catcactcaa gtctgtttca tctagttgac cagcaatcaa
    23941 gaaaaatcga gtggttatag gaatattacc gatcatttgc tggtaatctt gatagcggtt
    24001 agaaacgaca gcaggcccct ttgctaactc atctgtcaga gactgcgcac tatcatcgac
    24061 aaaatcatta aaaggcgcgc caattgctgt taatgtctga acttgtggca atgacgtatc
    24121 ctgtccatac gttcctaaat agcgtagtgc actaactcct cccatggaat gccccactat
    24181 gttgacttta tctatgccgt agttttcttt cagatacaaa agacaagttt tgatccattc
    24241 tgcttgattc cactcgttat ttttattatc ttcaaacaat acttggacca tcggattgtc
    24301 tgcttttcca gacaaatctc ctgttgcttg tatttgtcca tctgttccta ctgttaaaac
    24361 caattctttt actgcttgct gatttttttc caaacgccgc aacatccctt taaaggaatt
    24421 ttctgtcccg ctgtagccgt ggaaaaaaag tgttggaata gtggtttttt ctatcacttt
    24481 actggattct gccgtttttt ctttatcaga aacttcagta gtatttatct tcgtcgtctg
    24541 gcaaccactt aacgcaaaca aactgataag gactaaagct gctgcagttg cacgtttgca
    24601 taaatttttg attggttctg tcatacacct cttcctttcc atatagaaaa aagaaagact
    24661 tgaaaggcca atcatatgat cgacatttca agtctcttta ttaatcgatt ttatctactt
    24721 gatcgaaatc aagttctgta ctggtttcac gtccaaacat atcgatgttg actttcaatt
    24781 tttctttttc tccgtcaact tctgttactt gtccttctaa tccagcaaat gcgccttcga
    24841 tgattttcac gacttcgcca acttctactt ctagatcatt ttgtcgcagg ctcataccga
    24901 tagaacgcaa gatatgattg acttcttctg gaagtaatgg tgctggtttg cttccggctc
    24961 cgtgtgaacc aacgaatcct gtcacaccag gtgtgttgcg gacaacatac catgaagcgt
    25021 ctgtcatgat catttcaaca agtacgtatc ctgggaatgt tttatgaacg atttcttttt
    25081 ctttcccatt tttgacttca gtttcttttt cttcaggaac aacgacacgg aaaataaagt
    25141 cggccatttt catactttgc gcacgagatt cgatatttgc ttttactttg ttttcatatc
    25201 ctgaatatgt atgcaagaca taccagtttt tttcaaaaga ttccattgaa ggctcctcat
    25261 ttcattgtaa aataaaaaaa cctcagtaca cccgaagttt ttctttcgct tcattatagc
    25321 actaaaagta cgttgtggct agcgagttat tttaaaatcc agccaaataa gctttggatg
    25381 gctgtatcca ttacgtaaaa catcaatccg aaaataatag aagtttcgat gactactaac
    25441 gtatctttac ggagttgttt tctgcttggc caagttactt gtttgatttc atcttttacg
    25501 ctttttagaa atttcatgag acaacctccc ttgctactgt ttatttcgtt tctttatgta
    25561 atgtatattg attacaatat ttacaaaact tattgatttc caaacgttcg ccgctttttc
    25621 cttcactgac tgattttgtg tagttgcgag acccacaaac ggaacaagcg agtgctgctt
    25681 ttttctttgc catttacttc tgcccccttg ctgagaatca gacttatact tcataacagt
    25741 atcacgtaac tttttacttg tcaatttgtg aaataaacta tctattgttt tggataaaat
    25801 tatgatatga ttaatttgaa attaagctaa aatgagacaa ttggaggtat agcgatgcta
    25861 ttaaaaacac ttgtatataa gaaaaaagat ctgacaaccg ttacagagac tagtacatta
    25921 gaagaagcat taaaaatttt agaaacttcc ggataccgtt gtgtgccgat cttagattct
    25981 tcaggtaata ttttccgtgg aaacatttac aaaatgcata tctatcgtca taaagcaaat
    26041 ggcggtgata tgacgcttcc tgtcactcat cttttgaaaa acgctacaaa gttcatttac
    26101 ctggatactt ctttcttcaa agtattcttt acgatcaaag agcttcctta tattgctgtc
    26161 cttgacagtg aaaatcattt ttatggtatt ttgacacata gtacgttgct taatatgctc
    26221 tctcaatctt ggagcgtaga acaaggaagc tatgtactga caattgcttc cacaggaaag
    26281 caaggagatt tggctgctat tgcaaaaatc atttcaaaac acagcagcat tgccagctgt
    26341 attactcttg atatcgaaaa agacgaatac attcgacgta cattgatcac gctaccggct
    26401 aacactgaag cgaaagtatg tgaaacgatc gtcaaccact tagagaaaaa gaattataaa
    26461 gtggtagaaa ttgaaaattt gcaaaaccaa gaagcataat caaaaagcga aaaaagaaaa
    26521 cagctaatct gctttctttt ttcgcttttt ataattagac acgcttttca aaatcaagcg
    26581 cgcgatctaa ataatacatc aatggtgctg aaacagccat gacgataaac tcacttaatg
    26641 caagcgtgcc ataggttgtc caaaatgctt ggccacctgt tggaactaac atataagcaa
    26701 tcagtcccat gctgatggta aagaagatcg tgttcaatac taaacgcatc tttacatttt
    26761 tcactttgtt ttgcaataaa gctgtcaggc ctaaagaaag caatgtttgg ccgccaccaa
    26821 aaaccacatc catgatacca aaaccaaaga aggcattata gacgatgacc ccgcctaaaa
    26881 ctccccaaag caatttacgg ttaaagacaa ctaaatgatt caaactttct gaaatacgaa
    26941 actggatcgg ccctgaggat acaggcgcaa ctaaaatagt taacgctaga taaagagcca
    27001 tgatgatgcc atttacaacg attacggata accgttgtct gcttttcgtt ttttgcattt
    27061 tctctcctct tttctcctag tttttttaac gcgggatggt tgatgaaccg cggttatgtg
    27121 aaacaaacaa taattataac agacttttca tttatttcaa tccgtcttat ctgcttttaa
    27181 aaaagcaaaa gcttaaacaa tgtaaacgtt tgctaaaaat cgtgggatat ggtataaagt
    27241 aagtgtaagg ggtcataaga attcccagta aagcagacga agtaggatct gcaagaacag
    27301 aattgttgtg aaacttaaat aaataaaaaa ggatgtggga atttgagcaa cctcttacta
    27361 gagaacggtc tagcaagcag ctgaagatct aagtggacat gccgaacctt ggaacttctc
    27421 cttacgtaag taatggatca tgcgcttgct aggctttttt gtgtttttcg gtcttcggac
    27481 ctttttcatt ttcctagact tctgctataa taaccaagaa ttaattttgt aacagatttt
    27541 tttgaaaaaa caagtttctt tcattcaaaa aatagaaaaa gcttgctaat tattggttaa
    27601 ccagcaaaca ggagtattaa aatatcttgt tttattgaat agcaggagga aatcatgaaa
    27661 ttccattatc atatattgat caatccatcg gcaggcagcg gaaatggcta taaagtagca
    27721 gaaagaattt taccggtatt aaaaaataag catattgact atacacttca ttatagtgaa
    27781 tacaaaggac acgatgctga gattgctgaa actttggcaa aagaaacatt gattccttgg
    27841 atagaagaag aacagaaaac aatcgatact ttcccccttt tgatcatcgt aggaggtgac
    27901 ggcaccttac atcaggtact tgatactttc tatcagatgg aagtagaatt cccagttgct
    27961 tatattccag caggttctgg agatgatttt gctcgagggg caggccttcc aaaaaatccc
    28021 aaaaaaggac ttcagctgat tttagcagct caatcaccag aaaaagtcca cataatggct
    28081 tatgaagaaa aaataagtga aaaaaaagga atagctgtta ataatttcgg tataggatta
    28141 gatgcagcta tcgtccatgc gacaaatcat tcttccacaa aaaagcgatt gaacaaatac
    28201 aacttaggat cattttcgta tttattctct attctccgtg ctttattcac tcaaaaagga
    28261 tttccaattc tggtagatgc aggaggaaaa caattaagtt tttcaaacgc atttttatgt
    28321 acagcaacga agcatccttt ttttggcgga ggaatcgcga ttgttccaac tgctgatgcg
    28381 tctaaaccag tcattgattt tgtcgtggtc gaacgaatca atttctttaa aatcttgtgg
    28441 ctcattcttt tacttataca gaaaaaacaa ttgaaatcga aatatttcca tcactacaca
    28501 acaaacagat tacgtatcgt ttcaacaaca ccgcaacatg gtcaagaaga tggagaagaa
    28561 atggaaaaac gaccttttga tatcacaata accacaacta aacagttgtt ttggtgttaa
    28621 taaaagaggt tttgtcagaa gctacatctg gaacaccgtt tattcggaaa aaaggactgt
    28681 gacacgactt ttgtcacagt ccttttttta ctatgcagtt aattctttgc ccaattgggt
    28741 tgccatcgat agtttctttc tttacccaaa ataaattgga tcattgaaaa caccgcagca
    28801 aaagcaacta agacacaacc attgaaccaa aaagattctg caaatgtttg attgtaactg
    28861 agtgtttcac tgatcaagtt attattttga acaaaccaat atgttggtgt gaatgccgct
    28921 attttgctaa ccatttctgg caagtattct gctggtacaa acaccccacc gataaagcaa
    28981 ctgcctgtga taaaaatatc tttgatccca ccgctggttt ctgagttttt taccaatgaa
    29041 gtgatacaag ccgaaaagct aatgatcggc aataaaaaca agaaagtatt taaaatatga
    29101 taccataccg ttatatccca cgaggtgtgg atcactaaca gaaccattag aagtaagaag
    29161 ccactgaaca acagcagcga gtaactaatc aaactaaagg ttactttgcg attcaagttt
    29221 cgtttggtaa tgggcgcact ctggtttcgt ttactgatct ctttgcgatt aaatgctaga
    29281 ttgatgacgc tgaaaccgct aaaaatggat agaaacaacc cataagacaa gaaattaaag
    29341 gttgtgcccg tcaacagctt tttcatccgc tgactatatc ccgcacttag ctcaacttct
    29401 ccagtaaccg agagtgccat ttgtgtctgt tccatagctg catcgattgt tttatcttct
    29461 tcttgaaaca gaatcagtgt ttgcaaaaag gtgttcactt gctgtgtaac aactgtttta
    29521 gaaaaggcat ctgggcgact ctgtatcttg atcgtcggca tctttcctac ttcgacttgt
    29581 tcggtaaagt ctgcaggcag atgtaagata atgtttacac gattaaaata aagagcatca
    29641 tcgatttctt tttgtgaggt tccatccaat gtaacgactg tttccttgtc attcaaatat
    29701 ttacgaaggc catcgactac aggcgactct tcttcactaa tcaaggcgat tttcgcatct
    29761 tgcagagagg tattttcaac atcaatattt tgcgcaaaaa agaatgtggc aacaacagtg
    29821 ataattacac ctatcagtaa attcgattta tttgctttaa gcaatgtcca aatcgtttta
    29881 taaagcgtca taacgcaccc ctctttccat ccaagcattt cctaacgcaa aaaggaaaat
    29941 aaatccgatc aaccatagaa gattggtata aaaactagcc aaatcagtgt agaaataaag
    30001 tttatacaaa ctttcgctga tcaagttgac gagatttaat tgaccaacaa tcggaatatg
    30061 aagatcaatc caatacttca cttgttccga ccccatcata ccagcaaaaa agctcatcgc
    30121 aatcgtcact gaaatcaaga tgctatcctt ttgcaccaga ttcatttttg gtatcaaatt
    30181 accaaccaat gtacctaaca aaatcgcaca caacgacccc agagcggtaa caatcatcaa
    30241 ccattgccaa cgttcaccaa agtctacttg atagataaaa cgaaagacag ctaacataat
    30301 ataaatctct gtcaaaacaa tcgtaaagct tgctgccaaa ttagcgattg aaactaacga
    30361 ttgctttcta ggaatcaagg acaagcgaat accatttgac gattgatttg cttgctgatc
    30421 ctgtacattg cggacccccc acatcgtccc gtagagaatc gccattccaa tcagagtgaa
    30481 gaaataaaaa cttttgaaac tcatattacg acctgcatga acttcttgaa aagatacttc
    30541 ttgttcaaaa acagtcatga tgggcgcgag atcaacgttc tccacttctg ccactttact
    30601 cgaaattata ccaatacgct gtaaaaattg atccatgacg tttttcatca acgtctgact
    30661 cattcctgct tgacccacat ggagcgaaac agatgaacca tcaatgagat aaaaaccagc
    30721 caaatcatct gcatttagct tttcattcgc ttctacttcc gatagttcct gtacttcaaa
    30781 taatggttta tcattgactt cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc
    30841 tgtttgatcc gctttgatta caattccagt attaatcgtt tcgagattgc caccttgatc
    30901 aatctcgcca aaggccgcca taaacatcaa tcctaaaaga attggaaagg ccaatgtcca
    30961 aaacaacatg gatttatttc gcagcagtat ttttgtgcga tatttataaa aatgccaaaa
    31021 cattatttcc cctcctctag tctctcaaat ccttgccagt aatctctaaa aagacatcat
    31081 tcaaggacgc ttctttggtt gctatttttc cgtaactgat cttttcttct tgtaagattg
    31141 taagaatcgg caacaatact gtatttagtt cagtagttgt tacttggagc tgaccttttt
    31201 tataagcgat tccatcagaa gctattgttc gctgacaaca cgttttctgg gagtaatggg
    31261 atgtcgatcg tcaaacgatc cgccgtccgt accatatctt ttagagattc tttggttcct
    31321 ttagcaatca cctgtccttg atccatgatc acgatctggt cgcaaagaat ttctacttct
    31381 tccatgtaat gtgttgtata gacaatggtt gctccttgtc gattcagctc cttgatactt
    31441 tctaaaattt tattccgact ttgggggtct acagctactg tcggctcatc taaaaaaatt
    31501 aaccgcggct tgtgggcaat cccacatgcg atgttcaacc gacgtttcaa gccaccacta
    31561 agttctcctg gacgaaacga aacatatttt tctaatccca ctaattgaat cacctcttca
    31621 atcaaggctt tccttttctc tttttcggaa acatataaac cgcaaaaata atcgatattt
    31681 tcttgcacac ttagttcgtc aaagaccgca acgtcttgag gaacgacccc aatcattgcc
    31741 ttgacagcat aattttcagg agtcattgtt gtcccgaaca atttaatggt tcctttgtca
    31801 taatttaaca aagaaagcat acagttgatg gcagtcgatt tcccactgcc gtttggtcct
    31861 aacagaccta agacttctcc ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa
    31921 tctccatagc gtttgactaa tttatcaatt tcgataatca tcatgaaaaa ctccttccaa
    31981 atttcttgtt gatctgacta taataaagtg caagaaaaga aactagtgac gattggctaa
    32041 accacacatg acatttgtca caatgaagga gtctgctatg cataaatctc ggctactcaa
    32101 cgaaactagc tttctactgg ttatctcatt gcttttgctg gtaagtcaag gattcgttgc
    32161 tatgcaagtt atctatttat tgatcgccgt tttattcact ggtgtcagtt atttactgcc
    32221 tacaaagcca aattggattt tccaagttat tctatttctt ttatgtgcct ttttacctga
    32281 tcttgtcctg tttctgccag cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga
    32341 ttacttgttt ttgttaggtt ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa
    32401 ggtcgccttt gtcgtaattt tgctgttttt ttccttttac ttacgaaaaa aagatcaacg
    32461 aacgaccgat cttgagaaaa agtacattgt ctccaaagat actcattggg aaaaacaaca
    32521 agagttatcg gaacaaaata aagcattggc cctgtcacaa gaaacacaat tagccttgca
    32581 aattgtggaa gaacggaacc gaattgcccg agacatccat gacaatgtcg gtcatctgct
    32641 gtcaagtgcg attttacaaa ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa
    32701 agaaccatta gaaaatttaa gtgccaccgt tcacactggg atggatcgga ttcgtcaaag
    32761 tgtccatgac ctccatgaga catcgatctc ttttcaacac tccttagagt ttctgatcgc
    32821 tgattttcct tcttctgtta cgattgaagg aaacttgttt gaaggattga atgaaacgca
    32881 gcaaaactgc ttcttaatgg tcatcaaaga agccttaacc aacagtatca aacatagtca
    32941 tgccaaaacc gttgttttac attttcgaac gttgccggcc ttttatcgct tgcaaatcac
    33001 gaatgatggc accagcccac aaaaaaattc tgctcccggt attggtttga aaacgatgcg
    33061 tcaacgtgtt caagaacaaa agggacaagt ccatattttc caagaaaacc agcaatttca
    33121 gttaacgatc attttaccaa gggagggaca ccaatgataa cagtagtcat tattgagatg
    33181 atccatttgt gacgaaatcg ttacaaacca ttttagaaag cacgaaagag atccgtgtgt
    33241 taggcattgg tcattgtgcc aaggatgcgc tagtcttata cgaaacccat cgtcccgatg
    33301 ttttgctgac ggacatccgc atgcctaaac aaacgggcat cgatgctgcc aaagaaattt
    33361 tggctaaatt tcccaaagcc atccttttgc ttttgacgac attcaaagat gaagaataca
    33421 tccgcgaagc attttccatc ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga
    33481 ttattccttc tgtcaaatcc gcttataatg gacaggccgt atttggtaat gaaatcatcg
    33541 agactttcac tcaaatgatg aagaataaac cgacgattga tagtcatgct tatgcttctt
    33601 ttagtgaacg tgagcttgca atcatcaaag aagtagctgc cgggaaaaac aataaagaaa
    33661 ttgcagatgc cttgtacttg agtgacggca ctgttcgcaa ttacatcagc caattactag
    33721 aaaagctaga tttacgggat cgcacgcaat tggctatcta tttttatcag catttgcaat
    33781 aagtcttatc taacacaaaa aaacctgaat gatgctcctt cgcatcattc aggttttttt
    33841 cacatcttct attcaattgt cccgcaaacc atttcctcac gattgaaaga atcaaggatt
    33901 cgcagggctg ccaggattcg ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt
    33961 gtctaacgtt ttttctagtt ttgattcttc acgatcgtct cgttctacat caggactttc
    34021 attcaatttg aattcttttt ccatctcatc taacttttta aagtcattgc cagattcttt
    34081 tgctaatttc gctaatttat cttcggtttc tttagccata aagtaacgcc ccccttttga
    34141 tttcagctta gcatatcaca ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt
    34201 agctatttct catctttaaa atcaagcatt ttttgccggt ataaaatcgt agggagaact
    34261 gttttccata ccaatcatcg gatctactaa ctggttatcg atcacttctg ctgtaagaac
    34321 gaaatcttta gaaacagcag gttctacctc ttctttcttt tcttctccaa caaatgaaga
    34381 aaacagatct ggttccactg tttccattac ttggtttttc gtttcattcg tcccactgtc
    34441 atcttctaca ggtagctttt cttttacaac ccgtggctct tttgtatagt tcccatcttc
    34501 aaatggatca tttccgacca aagaatcttc ataagctgct agtttggtcc ggagagtgat
    34561 attcatttta tcattatcca tgatccttga tttcaacgta gtcatccggc ttgctgattc
    34621 attcgctaca caagtgacat aggcttgctc ttcccctaac aaaatcaaca gttccttact
    34681 gcgagtcact gccgtgtaga gcaaattacg ctgaagcatc cgctgatatt gatggaccat
    34741 gggcaaaata accatcttga attcactgcc ttgtgattta tggattgagc aacaataaga
    34801 aagagtgatt ttattccatt cgtttctttt ataagtgacc tcatttgcat caaactggat
    34861 aactaattca tccactttat cttcagaatc ttttgccaag atgatacccg tgatttctcc
    34921 catatctcca ttgaatacat tcaattcggg agtattgact aattggagga ctttgtctcc
    34981 tatccgataa acggtgtcgt tccaatgtac ttcctttttt ccaggtttcc ctggattgaa
    35041 tatctcctgc atcattttat ttaacgcatc gatccctgca ggtccccgat acattggtgc
    35101 taatacttgg atatcttgtg cacggaatcc cttatttttc gctttttcca caattttaga
    35161 gatcaagggt tcaatttgat aagcatcact tcggaaatag gaacgatctt tttgattctt
    35221 tgtgaaatct tttggcagac gtccttcttt gatctcatgg gcaagaggaa taatactaga
    35281 accatcacct tgccgataaa tctgattgag ttccattttg ggaatttgtt cgatttccaa
    35341 aagatcatgg agtacttgac caggaccgac agagggaagc tgatctttgt ctcctactaa
    35401 gataacttgc atattcgtcg gaatcgcttt gaataatgtg ttagccagcc atgtatcgac
    35461 catagacatc tcatcaacaa tcaaaagtcc gccttctaat tctttcgtac tgactgaagg
    35521 tgttttttcc cgtccattca gccccagaag acgatggatc gtactggcag gcagacctgt
    35581 cgtttcgttc attcgtttag ctgctcggcc agttggtgct gcaagtagaa taggaaaaat
    35641 ttcttgggta tattttgaag gatccaattc cagtccgttc aactctgcaa aaagattgac
    35701 gattccgttg atcacagtag ttttccccgt acctggtcct cctgttaaaa taaatagagg
    35761 ggaacggatt gcttcagcga tagcttgttc ttgcgaaggt ccatactgta tgccgaatat
    35821 tttttcgata cgccgaatat tcttctgcac ttctttttca ggataatgga tttctttttt
    35881 acgactgaga aggcgctgga tcgaattacc gatcccccat tcagaaaagt acaaactgtt
    35941 ttcgtaaaga ttagtctctt cttgttgtat ctttccttct tcaacaagtt ggataattac
    36001 agtagcaacc tgatcaggct ggatttcaat tgggcgactt gtttctaaaa tacggatcgt
    36061 ttgctgaagc aatgtctccg ccggtacgta tgtatctcct gtttgaactg catgctgaaa
    36121 aatctgatgc aagatagctg ctcgaattct tctcggtgaa tctgctgcaa taccgatttg
    36181 ttcggctaga ttatctgcac gtttgaaccc gattccttct atgtcttcga ctagctggta
    36241 cggattttct tcgatgatat ccaaagactc gttcttatag gcctggtaga tactgaaagc
    36301 taactgacta ccgaacccgt aacgattcag tcccatgatc acttgatcca tcccgtggtt
    36361 tagccgaatc gtctcagcaa gcatttctct ttttggcttc gtcaaaccgg agatttgttt
    36421 taataccgaa ggatcagcaa tgattttttc gatagcatct tctcccagca attctacaat
    36481 tttttctgct gttttcttcc cgattcctgg gaatttttcg ctggaaagat acgctacgat
    36541 cccatttgca gttgttggct tttcttgctc atatctttcg agtttaagct gccttccgta
    36601 tttaggatga tccactaatt ctccataaaa acgataagtc tcattttctt gaatatcgcc
    36661 gaaacttccg gtgactacaa tctctttttc acggtaatca gctgtggttt cactgatgtg
    36721 taccaaaagc actttgtaaa aattactagg attttgaaaa aaaatcgcgg caactgtacc
    36781 aacgaaataa ggcatttctt ctggctgcat cggaagccgc ctccttcttc tatagtaaag
    36841 gttctggtgc tgtttcagtt cctaaaaaat ctgtttggtt ccatactttc agttcatatg
    36901 gcaataatct gcaaggctct tcagtctcta aaatcgtcaa actactattg aacaatccac
    36961 ccatttcacg caactcactt agctcttttc ctgtcatcca ttgaatggct gcagttaaag
    37021 aagctccatg tcctacgaat aaaagtgggc catctttatg ttgggcaacc gcatctgcta
    37081 caacagtttc gatacgtgtg attgccgatt caatcgtttc tccgtgaaaa ggagaaggat
    37141 catacagatc taaatgatta cgcaaattcg gcagattatc cggatatttc tggtacattt
    37201 catcgatcga ctgaccttct aaagtaccca gccctagttc tcttagctta tctgtataaa
    37261 aaatctctac cggacgagca agctctttgt tgataccttc tgctgtatca cgtgctcgct
    37321 ttgctgtact ggaataaatt gctttaaaag gaacatattt tacctttttc ccaaatgctt
    37381 tgatttcttc gtaactttgt ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc
    37441 tttcctgatt ccattgtgtc ttcccatgtc gggtaaaata tagttccata cgcagcaaac
    37501 tcctgtcttt cttttatttt actctataat aaactgtcta atccagcttg tcattttcat
    37561 agtgatgcgt caattctaaa ttagggaatc cttgctggcg aagtgcttca tacactatca
    37621 ttgcagctgt attcgataaa ttcaatgagc ggacatgctc atcattcata gggatgcgta
    37681 aacacttttc ttcgttttca cgcataaatg tttctggcaa accggttgtt tcttttccaa
    37741 ataaaaagaa gtgctcttct ccatcagcaa aattttcgtc actatatact ttatgggcaa
    37801 attttgagat caaatgtagt ttacgattac ctaaaaatgt taaaaaagct tctaaattac
    37861 tgtggtaagt aatatttaca tcattccaat aatccaaccc tgcccgtttt agatgtttgt
    37921 catcagttga aaaacctagt ggctcgatta aatgaagcgg agtattcgtg gctgcacacg
    37981 tacgggcaat atttccagtg tttgcaggga ttaatggttc aaataaaacg atatgattca
    38041 tcaggatcat tcctctcata tttatgttcg tttctatttt ctgacaattt gatttttgca
    38101 taaaaaaaaa cgtatcaact cggtgtcagt cactgcgagt cgttacgtta gtgaagctct
    38161 tctcactaac ttttatcaga acaggaacct gataaaaacc aatgaaaaaa caacttgttg
    38221 ctaatataac accctcaact tttttttgca acctctactc attaaaaact gctagaaaaa
    38281 cgttttctct tagttccttt taataattct atgtcaattg taatcttctt caaaggattc
    38341 ttatataagt ctgctttgat tcgctcttct acctgccatt ctaaaggtga catggccaaa
    38401 agatcttttt gattttcctc tggtatctca aattcatagg ttacacgctg acgttcagtt
    38461 aatttcattg cttcagcaaa cttggctact acacgttcat tggaataact ctgtttttca
    38521 ggttgattcg gataaaaagc ctgacgtagt tcctttaaat aattttcttg tggcaccacc
    38581 ttcaacactt ctccatcttc tgagagtaca cgttggaatt cttggtaatg agaaggcgaa
    38641 aagatattta aaaccgcgtt gaatgtttga tcagcaaacg gcaaattggt caaatcagct
    38701 acacaccaaa atgcttcggc agattgttct gtagctgcgt agactccttc ttttgaaata
    38761 tcaaatccaa cagcattttg aatctttttc aattttgaaa gttcattcaa gaagctacct
    38821 tctccgcagc caacatctaa tagcgagact tcttcaggta aatgctgcgc aatagtctca
    38881 ataacgggct gatacatgcc agattggatc attctacgcc ttggaacgaa cattccttga
    38941 tcgtattctg ttttaaccgc atgggataaa aaataaagtg ttcctttttt ggaaaggtca
    39001 aaacggtgat tatttttgca tatcaagcca ttatcagact tttttacttc ttcttggcaa
    39061 accggacagc ggaacagctg atgattttct gccaaaaact ggcgtcctcg gtcaattttt
    39121 tttaacatag ttccccctca tttctgtttt atcttaccgt tagatgctca ctttggcaac
    39181 tgctaaaaat atgatacaat gcctagaaga aagtgaaaga taaaggagaa gattagatga
    39241 tcaaagaatt ttgcgctgaa aactacacat ctattcctct agcaattgct agaggagccg
    39301 atcggatcga attgtgtgac aacttagcgg tcggtggcac aaccccgagt actggtgtga
    39361 tcgaagaaac gttaagttat gctggggaaa aagatatccc cgtaatgacg attattcgtc
    39421 ctagaggggg cgacttcgtc tataacgata tcgaattgaa aatcatgcat actgatttga
    39481 ttgaagcaaa gaaattaggg acggatggcg tagtatttgg ttgtcttact ccttcaggct
    39541 ggctagacga agaggcctta gaactattga tcgaaacagc agaagggttg cagatcacct
    39601 tccatatggc ttttgacagt attcctgaag atcggcaatt cgaagcaatc gattggctgg
    39661 tagaacatgg tgttgaccgt atcttgactc atggcggtcc tgctgacaaa actattgtag
    39721 aaaatttccc acggcttaaa gaattgattg cctatacaga tggccgctta atcattttgc
    39781 ctggtggtgg tgtcactgca gagaatgcag cacatgtagc taaagaattg aacgtaagcg
    39841 aagtacacgg aaccaaaatc gtggatcttc agttataatc ttataaacaa aaaaacattt
    39901 gcaacaggca taatgcttat tgcaaatgtt tttgtttttt tacataaaat agcccataaa
    39961 taaagaagca atattgaaat aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa
    40021 gggaccgctt gctactgctg gatcaaatcc tagacgatcc attagcatcg ggattaaact
    40081 acctgcaaga ttcgccactg tgattgcaaa tagcatagcc atccctatga caaaccccaa
    40141 gataaaatta tgtttccaaa aacctacgat tagaaaaatc gtgatccccg taacagctcc
    40201 tgtgacaacg ccggtcaaga cttcagccaa aacaagtcga acaaatccat gatctttctc
    40261 atcagaaatc gcaagtcgac gaacagccac agctagcgac tgggttccag cattaccggc
    40321 agttcctgta atcagcgaaa tgaagactgc taaaatactc gcttcactta ccaagtcttc
    40381 atagtgactg attaaagaag cggtcgacat acctaaaaac aacagagtga tcagccaagg
    40441 caaacgtcga gacgctgcct ttactggatt ttcattaatt tcttccacat tgaccccggc
    40501 aagtcctgaa taatcgctag cagcttcatc atcaataacg tcgatgatat catcgactgt
    40561 gacgattcct aacaaatgat catcataatc ggtaacagga agagctaaga agtcataatc
    40621 tctaaatgtt tgtgccacat cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc
    40681 gctcatcaga tcagaaatca ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat
    40741 aacccctact agctgacctt cttgattaac gacatagatg taatagattg tttcagctac
    40801 atctgcttcg ttcttcagca cgtacatcgc agagcgaaca gtttgattgg ctacgatcga
    40861 aacaaattca gttgtcatga tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt
    40921 gatttcactg gcatcgtctg ccgacatcaa acttaaatat ttagcaattt gctttttatc
    40981 cagcgtatta agcaagtcaa cagcattatc tgtatacatc tcagaaagca tatcagctgc
    41041 atagctagga cgcatttcat ctaggtattc tttcatatgt tcgttgtctt cttcaatcac
    41101 atcgaacata tccgctaatt ctttaggcga cagataggaa tagatcagct gcctgttctc
    41161 ttctgtaaga gattggtaga attgcccctg ctcatagata tgcatctcta aaaaactgtt
    41221 tctgaatgcc tgaatattat tttcccttaa atcttgagtc aactgggcaa atctttcctc
    41281 taattcttga ttttcatcca aaccaatctc acctcgcctt attcttcaaa ccgtctcaca
    41341 actgcctcca tgtctgcggc taaaggctgc ttgaactcca gcaactcttc tgtaaatgga
    41401 tggtaaaaac gcagatcata acaatgaagg gcttgtcttt ttattcccag atccattcgc
    41461 ccgccataca tatcatcacc gagcagggca cagccaattg cggaaaaatg aactcttatt
    41521 tgatgtgtcc ttcctgtatg cagctggata tcaactaacg cgatttctgt tagtcgcttc
    41581 tctatccagt actcagtctg tgccgaacgt ccttcatcag agaccattcg cttcaatagc
    41641 gaagaataat ctcgggcgat cggcaaatcg atatgtccat gtgccgataa atctgaaagc
    41701 cttccactga ctaaggcctg atattttttc acaaacttct tttcacgaag ttgcatatct
    41761 aattttgcgt gcgcaaatcc atgctttgca aaaagcatca aaccagatgt atcccgatct
    41821 agtcgcgtca ccacatggat tacttggttt tcgtaacctt gttttttata ataagccttt
    41881 acacgattag ccattgtccc atttggatgg tattgggcag gaatagacga aacgcctgct
    41941 ggtttattta ctaccagaac gtgttcatct tcatacacga tatcgatcgg tgtttcatcc
    42001 aaaagtacgg tttcatgttc tccttcagca ggaatcacaa tggttacctt atcatcctta
    42061 gctagagaaa acaagacatt ttctacttga tcattaactt tgatttgacc gccttgaaat
    42121 tttattttcg ctaacagacc ctttgagatc cctttttctt ttaaaaaata ttttacttgc
    42181 tgagaatgat ctttctcata atcccatgta aactgcatat caatcctcac taataaaggc
    42241 atctttcaca cgatgccaaa aatgcatatg tcgataagaa gcaaaatgga tccgttcttc
    42301 tgctattcga taataaattg agcggatatt tgcttttgcg acattcaatt gatcaaccgt
    42361 tacaagataa tcttctgtat tttccaattt tatttctagc cattcatcat gggcaatcac
    42421 gatcggagaa cccaaagtac gaaacactcg attattcagt gaagcaatct ctgccaactg
    42481 aaaggcattg atactaggat ggatcacagc accgccgaca cttttattat atgctgtcga
    42541 accagtaggc gtagaaatcg tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt
    42601 gatgtataca tcggctacca tcgtacgatt ggcccgttta atcgttgatt cattcagtgc
    42661 taaaaaatgc tggtccggtg tttcatccaa ataactgatc cgaacatcca gcaagggata
    42721 actaacactt tgttcccggt tcgttcgtaa gctgtctact agttcttcca gctcgtaatc
    42781 ccgccaatcc gtgtaaaacc ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt
    42841 cagacaatga ctgtagtgat ggaacgccga caataatgtg ccgtctccgc cgactgagat
    42901 gaccaattct ggctcatgat catctcgttg aatccctgct tgttctaaaa gtgcgttcaa
    42961 acgttctgtc acttctatag tctttgcttc tttattatga acgatcgcta ctttcatccc
    43021 ctgcctcctc tctttcgctt tctttcttca aaagcgttcg atagtagaca tcatctgatt
    43081 ttcctcttcc atgggagaat aaccgctgtg cttcttggat ttcttcacgg atactggaca
    43141 tctcttcatc caactgatag gcagcttctg ccgcacgctg aagtcgttca ctcatttctt
    43201 ctggaaaaac gccttgatat ttataattca gcgaatgctc aatcgttgcc cagaaattca
    43261 tcgcaagagt ccgaatctgg atttccgcca aaattttctt ctcaccgctg atcagctgta
    43321 caggatactc gatcaccaga tgataagaac ggtagccact tgctttttta tttgtgatgt
    43381 aatctcgttc ttggagaact ttcatgtctt ttcttttacg gatgatctca acaacttgat
    43441 ggatatcttc tacaaactga cacatgatcc ttaacccggc aatatcagac atttcttctt
    43501 ctaaacgtcc cattggaata tgccgtaatt gtgattttgt tacaatacta tcgactggct
    43561 tgactctccc agtgacaaat tcaatcggga catgtcgatt ttgttcacgg aactgtttgc
    43621 ggatcccccg cagtttcact ttcaattctg atacagtttg ttcatatggt gctaaaaatg
    43681 cttcccaatc tcgttccata gctgatacct ctacttcatt aacattcatt gtttattcta
    43741 ccatatatgc aaagtttttt cgtactcttg atttatgcat tgaccaaaaa aagaaaatta
    43801 ggcaaaatag acaaaaagga gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat
    43861 ctatgctgac aaagcaagag tacgagatat tcatcgctca ctatcaatta acagataaag
    43921 atttccgtgt acagactaat atttatttcg ataccgttga tgaacagctg aagaaacaaa
    43981 attgtggcct acggatccgt ttcgttggtc atcaagctga atatacatta aaaacacctg
    44041 ctgaaaaagg gcgtctcgaa acaacggata cctttataac agaagaagca gaagcattta
    44101 tcttcgaaaa acgtctgcct cgttcaggcg cagtttttca aaaactttct gatttgaata
    44161 tcgatcctgc ttcattaatc caaaccggaa agcttacaac aaaacgggct gaattcccta
    44221 ttgaagaagg acttttagct attgatgaaa gctggggaga caatctacac gattttgaac
    44281 tggaactaga ggtaagggga tgcatctgtg ggaaaaattg cttttataaa ttttttaaaa
    44341 cgtttttctt taccttatcg tcctgctaag aacaagatcc aacgtatgct tgaagctaaa
    44401 aattagaaac ctttcatttt cacaaggcac ttgagttcat ttttttactt ttttctgtta
    44461 agttatagga tgtgaaagaa tctagtcact agttgtatag aatctatttt gggggagatt
    44521 catatgatcg aaatctattt atttgttaat cctttagggg aatactgttt cgaaatggaa
    44581 caacagttat tacaatttat cgaagaagag tacgggacac catcaaaaga aaagatgcaa
    44641 tttcgctttc ttcctttggt caatttgcag acaattgggg atgtcatgca acaaaaagga
    44701 atttctcaga acaatcttga ggaacgtaat cacttattct caatgactta ttctgcagca
    44761 ttggactgca aagcggcgca gtttcaagga aagaaaaaag gccgccgttt tttgatcaaa
    44821 ttgcaagaag cagtcggctg taatgacata ccatattctc aagaacttgc tgaatccata
    44881 tttgaagaaa tcggcggcga tttgaatatg tttaaagaag atcgtcagtc tgacttcgtt
    44941 aaagaagtat tcttttctga tcaaaaaatt gctcgggaaa tgggcatcac aaaacaccct
    45001 tctgcagtcg tttacaatta tgcctgcgac cgtgattttg gtgttttagt agaagtaaaa
    45061 ggtacaagta gcctttggag aatcacagag ctgtgtaaaa caacagataa ctcatatcaa
    45121 gtttttaatg gtgaacattt acatacaaat gattctcagt tccattcaag aactagccat
    45181 ttacacttgc tttctaatta aaaaaatacg tcgactccct tacaagagag ccgacgtatt
    45241 tttttcgttt ctattcgaga aaatcaaaaa tagaaagcct tataaacgat cgatcaattc
    45301 ttctaattca tttagacgtt gttcaaacat cgacattgca tcttcaatat atgcagcctg
    45361 tgtcatatca actcctgctt ttttcattac ttccacagga taatcgctgt tacctgcttt
    45421 taggtaagcc aagtagttct ctagtgcctc tggttcctgg tttagtattt tcttcgccag
    45481 tgctgaagca gcagaaaagc cagtcgagta ttggaaaaca tagtaattat aataaaaatg
    45541 cggtatccgt gaccattcga atttaatttc tgggtcttct tcgactgctg gaccataata
    45601 ttttgcattc aatttaccat aactatcact taggtattca ctagtcaatg gcacaccttt
    45661 ttcatcttcc gtatgcatga aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt
    45721 tcctttaaac ccatccaagt aatggttgag tacataagcc cgtacacgag ggtctttttc
    45781 tgtttccaat aaatactccg ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa
    45841 aaagatggag tagtcgccgt acacataagg ctggttcgaa cgagtgaaat aactatgaac
    45901 actatgtccc atttcgtgga caagcgtaaa tagctgatcc agcgtatcat gccaattcaa
    45961 taaaatatat ggatttgtgt catagcttcc cgaagaataa gcaccgcttc gtttcccttt
    46021 attttcgaca acatcgatcc aacgttcaga gaatgctttt tctacgatgg ccatgtattc
    46081 ttcacccatt ggtttcagtg cttctaaagc tttttctttt gcttcttcgt acgtaaaggt
    46141 aattggagct tcccctaaga ccggtgtata aagatcatac atgtgcagtt tttccacttc
    46201 taataaacgt ttccgtaatt ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac
    46261 gtctaccaaa gtatcgtata cactttcagg aatatgatta ttgctcaaag acgcttctct
    46321 ggcagagctg taattacgga ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa
    46381 agtagaagca aatgtatttc taaattgttc gtaaacactg tacaatccct taaatgctgc
    46441 ttcgcgcacc cttcgatccg tgctttctag caactgacca tacacgccat gagataattg
    46501 gactatttca ccattttctc cttcaatcgt tggaaaaact agatctgcat tattcaaaac
    46561 agcaaatgta tcacttgaag catcaaagat ttcacctgct ccagcaagaa gagattcctg
    46621 ctcagcagat aagacatggg ctcgattatc tactatttgc tggatataat ggcgatagac
    46681 ttccaatttt ggttcttctt tgaaatattg ccaaatctgg tcatctgaca attgcaatat
    46741 ttctggttca aaccaggaaa cggcttcact aactttcgaa aacagactgc ttgctcttgc
    46801 ataaagcgct tggtaatctg tatttccagt gtcttgatcg tttttaaggt gcgcatatac
    46861 ataaatgact tcagtttggc gataaaccct caatacgaat tcaatcgcat ttaaaaattg
    46921 agaagcgcct tgatcaagtg ttcctttgtg tttttcagat tgttttaact cttctgataa
    46981 ttccaaatat ttctcatcaa actcttggtc gcttgaaaag atcttggtca agtcccaagt
    47041 taaattttca ggcaattctt ctcgttttgg caactgcttt acttccatga aatccactcc
    47101 ttttcttccc ttagctctat ctatcaaaat agatcatagt ctaacatcca catgtcacag
    47161 catctaactt ttctactcat tcatgtgtct gcttccatca attttagcat aactaaatct
    47221 tgttttacat agaaaaccga ttcctagcct atttttatac ttactatttt ttcaaagaag
    47281 acaagtgtat gcattcgtta aaaaactgct gatagaccat ctcttcatca atcataggaa
    47341 aaagccattc ccttttatgt tcttttattt ggcaaagcca tgagttgaaa tcaggaactt
    47401 tctggttttc caaggcttct tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat
    47461 aaaagaaaaa ccgacttttt tgatagatcc acgatgatag atgcaaaaga tgtttatgac
    47521 gcaagtagca ttgttcttgt atggatactg tattcttttg cttgtgaata agttggcgtg
    47581 tcagccattt cttcccatct tcactcactt tttctatttt tttcatttgg ttcattttaa
    47641 tttcgttact agtgaatatt tctttaaggt ccctactaga aaaagtccag cagctactgc
    47701 aaaaggaaat atgccctgat acagtctctg taatatgatg atacaaataa agcttttgtt
    47761 tcttgagatc agctttccag caatgcactc ctctttttct attatagtag cagcaacttt
    47821 tttctatcaa tgagaattgc gattgtccaa gaaaagaatt accaagtatc caccatactt
    47881 ggtaactgtt attcaaataa ttttgggttc tttcaataaa tctctggtga gacagtctag
    47941 aacattggat ttctattgct agattcgcca acaaaatatc cggcctttgc cgcaacctcg
    48001 gcaaataggc ttccaaaaat actggttcta ctgactgtcc cagccaatcc atgaagcatt
    48061 cctttaactg taagtgctct tttgattctc tttcactaaa gaccgaacag ttatctgatt
    48121 tttgatgggc aaaatgagca cattttttct ttcccttttt caaaatcact ctttttcgac
    48181 agccacgaca ataatatttc tttccttttt ctgcatgttt agcaaaacag cacttattat
    48241 attcattcat tgcaagcagc atttttctcc ctctgaactt atttacgtct aagaatttat
    48301 ttacacgaaa aaagagggcg tgaaaaagct attctgcatc aagtaataag aacagctaaa
    48361 caaaaatagt tcccaatatt tttcgtttgg ttgggcttgt taccgcagat gcaagctttt
    48421 gaacacattt atacgaaaaa aagactgcga cacaattttt gtcacagtct cttactataa
    48481 tgctgattta tacaaaataa aatttcgttc gctcgatagc attacgcgcc atcagacatt
    48541 gtccatattc attcaatgtt tctggtgtac gatcgctctt ttcagcaaac tccaaaattc
    48601 gagcaatctg attctctaca tccgcttggt tgagattttc catccagaag tgaacgacta
    48661 ggtaatagcg ctcttcgaat aaatacaaat cagtccaagc agactctagt tcgacatctg
    48721 ttgcaagttg gatcacttgt tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt
    48781 cttctagggc gcttccagat aattcttcct ccggatcgct gccaataatc tgctgacgaa
    48841 gaaagtcact cacatcttct gaatcaacag tagaatcttc tgattgagaa atttcttcat
    48901 ccggaagatt tttactgata aacagttcta atccgtctcc ttttggcaaa acttggaaag
    48961 tgacagcttc gcttcctcgg aactcctctt cgatatcaac ttcttccaaa atactataaa
    49021 agaaattttc gatttctttg tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct
    49081 ctaaatcttc atgaccaatt agtacacgga tcgtattttc attaatatgt tccatttcca
    49141 ttttgctaca cctcactttg ccacaatgtt taatatagat tcattgtagc gaaatcagct
    49201 taaatgtaaa gaaaaaaaat ctcgctgtta agcgaattta ataaaaccag cggatttttc
    49261 aacctttcaa gctatcttat ttaactataa catttttggc agacgaatac tatatagccg
    49321 acttttcagc taattttccc tctctatttt tcgtgcaatc atcatcttct actacctttt
    49381 tatacatcta atcaagaata ctttttgtaa aaacaaaaaa acttggaaaa actatctttg
    49441 taatagtctt t
//
LOCUS       DAZKNH010000009        47433 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000009 DAZKNH010000000
VERSION     DAZKNH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.009"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..476
                     /locus_tag="ACG8MG_000488"
     CDS             <1..476
                     /locus_tag="ACG8MG_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0374562.1"
                     /translation="GKKELASVGMFFSAIVYLILFLIKASNMYVFLILSSVGYMGLGV
                     FNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVGGWTLSIIGYDS
                     LANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENCELLKQKHK"
     gene            486..1280
                     /locus_tag="ACG8MG_000489"
     CDS             486..1280
                     /locus_tag="ACG8MG_000489"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0374563.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            1293..1802
                     /locus_tag="ACG8MG_000490"
                     /pseudo
     CDS             1293..1802
                     /locus_tag="ACG8MG_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
     gene            1840..2397
                     /locus_tag="ACG8MG_000491"
                     /pseudo
     CDS             1840..2397
                     /locus_tag="ACG8MG_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            2455..4494
                     /locus_tag="ACG8MG_000492"
     CDS             2455..4494
                     /locus_tag="ACG8MG_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0374564.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFINNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4603..5754)
                     /locus_tag="ACG8MG_000493"
     CDS             complement(4603..5754)
                     /locus_tag="ACG8MG_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0374565.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(5908..6438)
                     /locus_tag="ACG8MG_000494"
     CDS             complement(5908..6438)
                     /locus_tag="ACG8MG_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0374566.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(6537..6887)
                     /locus_tag="ACG8MG_000495"
     CDS             complement(6537..6887)
                     /locus_tag="ACG8MG_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFQ0374567.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(7044..8573)
                     /gene="ahpF"
                     /locus_tag="ACG8MG_000496"
     CDS             complement(7044..8573)
                     /gene="ahpF"
                     /locus_tag="ACG8MG_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFQ0374568.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(8583..9146)
                     /gene="ahpC"
                     /locus_tag="ACG8MG_000497"
     CDS             complement(8583..9146)
                     /gene="ahpC"
                     /locus_tag="ACG8MG_000497"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFQ0374569.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(9290..9952)
                     /locus_tag="ACG8MG_000498"
     CDS             complement(9290..9952)
                     /locus_tag="ACG8MG_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0374570.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            10136..11782
                     /locus_tag="ACG8MG_000499"
     CDS             10136..11782
                     /locus_tag="ACG8MG_000499"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0374571.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            11773..12855
                     /gene="uxuA"
                     /locus_tag="ACG8MG_000500"
     CDS             11773..12855
                     /gene="uxuA"
                     /locus_tag="ACG8MG_000500"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0374572.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(12963..14516)
                     /locus_tag="ACG8MG_000501"
                     /pseudo
     CDS             complement(12963..14516)
                     /locus_tag="ACG8MG_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            14664..15830
                     /locus_tag="ACG8MG_000502"
     CDS             14664..15830
                     /locus_tag="ACG8MG_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0374573.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEETETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPTLSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHLTPYSQRQTIEQDSAID
                     SGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEIT
                     PGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPE
                     PDTDARDQGIPEKINKKTIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETL
                     KSSKDTTFASPETKNKQLPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(15956..17035)
                     /locus_tag="ACG8MG_000503"
     CDS             complement(15956..17035)
                     /locus_tag="ACG8MG_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0374574.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(17144..17584)
                     /locus_tag="ACG8MG_000504"
     CDS             complement(17144..17584)
                     /locus_tag="ACG8MG_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0374575.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(17641..18051)
                     /locus_tag="ACG8MG_000505"
     CDS             complement(17641..18051)
                     /locus_tag="ACG8MG_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0374576.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(18232..18666)
                     /locus_tag="ACG8MG_000506"
     CDS             complement(18232..18666)
                     /locus_tag="ACG8MG_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0374577.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(18766..19614)
                     /locus_tag="ACG8MG_000507"
     CDS             complement(18766..19614)
                     /locus_tag="ACG8MG_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0374578.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            20003..21769
                     /locus_tag="ACG8MG_000508"
     CDS             20003..21769
                     /locus_tag="ACG8MG_000508"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFQ0374579.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            21871..23130
                     /locus_tag="ACG8MG_000509"
     CDS             21871..23130
                     /locus_tag="ACG8MG_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0374580.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            23277..24578
                     /locus_tag="ACG8MG_000510"
     CDS             23277..24578
                     /locus_tag="ACG8MG_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0374581.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            24581..25432
                     /locus_tag="ACG8MG_000511"
     CDS             24581..25432
                     /locus_tag="ACG8MG_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0374582.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            25445..26260
                     /locus_tag="ACG8MG_000512"
     CDS             25445..26260
                     /locus_tag="ACG8MG_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFQ0374583.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            26411..27577
                     /locus_tag="ACG8MG_000513"
     CDS             26411..27577
                     /locus_tag="ACG8MG_000513"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0374584.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            27807..28454
                     /locus_tag="ACG8MG_000514"
     CDS             27807..28454
                     /locus_tag="ACG8MG_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0374585.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            28538..29482
                     /locus_tag="ACG8MG_000515"
     CDS             28538..29482
                     /locus_tag="ACG8MG_000515"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFQ0374586.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            29617..31068
                     /locus_tag="ACG8MG_000516"
     CDS             29617..31068
                     /locus_tag="ACG8MG_000516"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFQ0374587.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            31089..31925
                     /locus_tag="ACG8MG_000517"
     CDS             31089..31925
                     /locus_tag="ACG8MG_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0374588.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            31925..32584
                     /locus_tag="ACG8MG_000518"
     CDS             31925..32584
                     /locus_tag="ACG8MG_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0374589.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            32710..33399
                     /locus_tag="ACG8MG_000519"
     CDS             32710..33399
                     /locus_tag="ACG8MG_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374590.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            33405..34361
                     /locus_tag="ACG8MG_000520"
     CDS             33405..34361
                     /locus_tag="ACG8MG_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFQ0374591.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            34405..34917
                     /locus_tag="ACG8MG_000521"
     CDS             34405..34917
                     /locus_tag="ACG8MG_000521"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0374592.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(35173..35721)
                     /locus_tag="ACG8MG_000522"
     CDS             complement(35173..35721)
                     /locus_tag="ACG8MG_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0374593.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(35810..36991)
                     /locus_tag="ACG8MG_000523"
     CDS             complement(35810..36991)
                     /locus_tag="ACG8MG_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0374594.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(37227..38735)
                     /locus_tag="ACG8MG_000524"
     CDS             complement(37227..38735)
                     /locus_tag="ACG8MG_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFQ0374595.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            38894..39085
                     /locus_tag="ACG8MG_000525"
     CDS             38894..39085
                     /locus_tag="ACG8MG_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374596.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            39288..40205
                     /locus_tag="ACG8MG_000526"
     CDS             39288..40205
                     /locus_tag="ACG8MG_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0374597.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            40198..41271
                     /locus_tag="ACG8MG_000527"
     CDS             40198..41271
                     /locus_tag="ACG8MG_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374598.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            41419..41937
                     /locus_tag="ACG8MG_000528"
     CDS             41419..41937
                     /locus_tag="ACG8MG_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374599.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            41918..42310
                     /locus_tag="ACG8MG_000529"
     CDS             41918..42310
                     /locus_tag="ACG8MG_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFQ0374600.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            42386..43321
                     /locus_tag="ACG8MG_000530"
     CDS             42386..43321
                     /locus_tag="ACG8MG_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0374601.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            43454..44233
                     /locus_tag="ACG8MG_000531"
     CDS             43454..44233
                     /locus_tag="ACG8MG_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0374602.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            44226..44543
                     /locus_tag="ACG8MG_000532"
     CDS             44226..44543
                     /locus_tag="ACG8MG_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0374603.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            44787..46397
                     /locus_tag="ACG8MG_000533"
     CDS             44787..46397
                     /locus_tag="ACG8MG_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0374604.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            46554..47402
                     /locus_tag="ACG8MG_000534"
     CDS             46554..47402
                     /locus_tag="ACG8MG_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0374605.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        15792 a         8415 c         9149 g        14077 t
ORIGIN      
        1 atggaaaaaa agaactggca tcagtaggaa tgttcttttc agcaatagta tatttgatct
       61 tatttttgat aaaagctagt aatatgtatg tattcttaat tcttagttcg gttggctata
      121 tgggtttggg tgtgtttaat actgttattt gggcaaatat tactgatgtt attgatgatc
      181 aagaagtaaa gtctgatcaa agagaagatg ggaccgtata tgcggtatat tcttttgcta
      241 gaaaaatagg tcaagctcta gctggaggcg tcggaggttg gactctttct attataggct
      301 atgactcttt agcaaatgta caaacgggag atgttttaga aaagttatat aacgcttcaa
      361 cattaattcc atcaatttgt tttctgattg taggattaat tttatttttc ctttatccat
      421 tatctaaaga taaagtctta gaaaattgcg agcttttaaa acaaaaacat aaataggaga
      481 gaaatatgaa taattggata ttttgtattg attcagatgg ctgtgttatg gatacgatga
      541 cttataaaca caatctcttt ttcggaccaa ttgctgcaga gatttttgat gttcatcata
      601 gagaaaaatt cctacaggat tggaataata ttaatttata ttcagagact cgtggaataa
      661 acagattttt aggtttagtt aaaacattag aaaaaaatga tgtaacatcg attaatcgat
      721 taaagcactg ggtatctcta acaaaagaat tgtctaatcc atctttgaag agggaaatag
      781 aatattctca gtcagaggat ttattaaaag ctttaaaatg gtcggaagaa gtcaatcaac
      841 agattagtca agcaaaagga ttagataaac cctttttaag tgctagagat actataaaag
      901 ctctgaaaaa atatgggaag ataattattg taagctcagc taataaggaa gctgttcaag
      961 aagaatggga aagacatgaa ttattatcat tagttgatga actgtgttgt caagataaag
     1021 gaaaaaaaga agatattatt cgaagtgtaa ttgaaaatgg ttgtgatttg gataagatat
     1081 taatgattgg tgattctcct ggtgatctag aagctgcaaa taaaaataaa gttttctttt
     1141 atcctatttt ggtcaataaa gaaaaagaat catgggaaaa cttaagaact gatgttttat
     1201 ttaaatgttt atcgggagat tatgtagata ttgaacaaaa atatatagat actttttgga
     1261 aaaatcttac taataagtag aggagaaaga aaatgactat aaaagtagat ttacgaaaaa
     1321 aaccatataa tttagatgaa gatgctattt cttggattga agagacgatt gattctatga
     1381 ctttggaaga aaaaataggt caattgtttg ttaatatggg atctagtcgt gataaaaatt
     1441 acttgagtaa tatggtcaat cagtaccata ttggagctgt acgatataat ccaggaactg
     1501 ctgatgaggt atatgatcaa aactatatct tacaaacaga aagtaaaatt cctctattaa
     1561 ttgctgctaa tacggaagca ggtggtaacg gagcttgtac agatggtact gaagttggtg
     1621 tagaagtaaa aattggtgca acaaatgatg cgaaatatgc ttatgaatta gggagagttt
     1681 ctgggattga agcatcggct attggatgta actggagttt tgctcctatt gtagatatta
     1741 atagaaattg gagaaatcct atcatttcta gtcgatcttt tggacaaaac cctgataaag
     1801 ttgaggagga gatgcggtga aaaaagatcg attggtggag gtcgctcgga ttcacttctg
     1861 gttgcacaat acaaccagaa aacacaacaa gcaaaactga tctctatcat gagagattca
     1921 tatgtcgata ttccaggtta cggaatgaat aagataaatg cagcttactc ttatggggga
     1981 gttgatttat taaatcaaac attaaaggaa aatttcaaat ttgaagctcc ctattatgca
     2041 agtatcacat ttcaagattt tatcgattgc gttaatgaac tgtttcctgg tggagtcaag
     2101 atcgatgcag aaaaatctct agatttagat ggcgtatata taaacaaagg agagcaagtg
     2161 atggatggta atactttact ccagtatgct cgatttcggg aagacaaaga aggagacttc
     2221 gggaggatca gaaggcagca gcaagtgatc aaagctatct ctcagcagct gaaagacgta
     2281 acttcaatat tcaagctacc caaagcggtt gggaaattac ttggtagtat ccaaacgaac
     2341 cttccagaaa gtgtgcttct ggattgtggt atggatttcc tcaaagacat atgttagaat
     2401 aaacaggtat aaatagtgaa ataaaggaat gacaagcaag agaaggagga aaaaatgaaa
     2461 agaagtgaca agcacggcaa aaatcgaaca ggcgcttata ttgccggcgc agtgatctta
     2521 atagcagctg cgggtggcgg ttatttttac taccagcact atcaagaaac ccaagcagta
     2581 gaagctggag aaaagacagt tgagcaattt gtccaagctt tgaacaaggg agattataac
     2641 aaagctgcag aaatgacatc aaaaaaggcg gcaaataaaa gtgcattatc tgaaaaagag
     2701 atcttagata aataccagaa tatatacggt gctgctgatg tcaaaggact tcagatatcg
     2761 aatctaaaag tagataaaaa agacgattct acttacagtt tttcatataa agcaaagatg
     2821 aatacctcat taggtgaatt gaaagatctt tcttataaag gaacattaga cagaaatgat
     2881 ggtcaaacca cgatcaactg gcaacccaac ttggtttttc cagaaatgga aggaaatgac
     2941 aaagtaagcc tgaccacaca agaagcagca agagggaata ttatagatcg aaatggtgaa
     3001 ccattagcaa caacaggcaa actaaaacaa ttaggagtcg ttccaagtaa acttggagat
     3061 ggaggcgaaa aaacagccaa tatcaaagcg attgcttcct ccttcgactt aacagaagat
     3121 gctatcaatc aggcgatttc acaaagctgg gtacaacccg attactttgt cccattgaaa
     3181 atcattgatg gagcaacgcc agaacttcca gctggagcta ccattcaaga agtagacggc
     3241 agatattatc ctttgggtga agcagctgct cagctgattg gttacgtggg agatatcaca
     3301 gcagaagata ttgataagaa tccagaatta agcagtaatg gaaaaatcgg tcgctcaggt
     3361 ttggagatgg cttttgataa ggatcttcgt gggacaacag gtgggaaatt aagcattaca
     3421 gatgcagacg gtgtagagaa aaaagttctg atcgaacatg aagttcaaaa tggcaaagat
     3481 atcaaattga caatcgatgc gaaggcacaa aaaatagctt ttgacagtct aggaggaaaa
     3541 gctgcatcaa ctgttgcgac aacgccaaaa accggtgatc ttcttgcgct tgctagctct
     3601 ccaagctatg atccaaacaa aatgacaaac gggatctcac aagaagatta caaagcttat
     3661 gaagaaaatc ctgaacatcc attcatcagc cgatttgcga caggttatgc tcctggatct
     3721 acgtttaaaa tgattacagc agcaatcggt ctcgacaacg gcactatcga tccaaatgaa
     3781 gtgttgacga tcaacgggct taaatggcaa aaagacagtt cttggggatc ttttaaaaca
     3841 acgcgtgtca gtagtgatgt atcacaagta gacttaaaaa ctgctttgat ctattccgat
     3901 aatatatatg cggcacaaga aacgttgaaa atgggtgaga aaaaatttcg tacaggctta
     3961 gataaattca tttttggtga agaccttgat ttgccaatta gtatgaatcc agcacaaatt
     4021 tctaatgaag atagctttaa ctcagatatc ttgctagctg atactggata tggacagggc
     4081 gaacttctaa ttactcctat ccagcaagca gcaatgtatt ctgtttttat caacaatggc
     4141 acacttgtct atcctaaatt gattgcagat aaagagacaa aagataagaa gaatgtcatc
     4201 agcgaaacag cagtacaaac gatcgtgcca gatctgagag aagttgtgca agatgtaaat
     4261 ggtacagcac attctctttc tgctttaggg attccgttgg cagcgaaaac tggtacagcg
     4321 gaaatcaaag aaaaacagga tgtaaagggg caagagaaca gtttcttgtt tgctttcaat
     4381 cttgataacc aaggttatat gatggtcagc atgttggaaa ataaagaaga tgatgattca
     4441 gcaaccaaac gagcatcgga actattgcaa tacctcaacc aaaattatca ataaaaaatt
     4501 gctttttaag tacagtaaaa ggactgtggc gttatcgtca cagtcctttt ttcaaataaa
     4561 cggttttcac gatgtctctt tcacaacttc tttttgattt actcacactt cataaacttt
     4621 ttttgtaaaa tatttgagaa caagtggaga aataagtgta cttaataaaa tcacgataac
     4681 taaaggtgaa tagtactgtg gttcgatcaa ttgactttgc tgtccaattt gtaaaatgat
     4741 caaagccatt tctccacgcg aaatcatacc agcaccgacc atcaatgaac tatgttgaga
     4801 aaaaccggca atctgagcac caaaataacc tccaagcaat ttggtcaaaa tcgctacaag
     4861 cgtcaatata gaaataaaga gtagttgttc actaaagtca gaaaaatcaa cttctaatcc
     4921 tacactgaca aaaaatactg gtataaatac tgcatatcct aaagcctcta cattgtggta
     4981 tacttcttct ttaacctttg tctgactgac agctatccca gcaaaaaaag caccaatgac
     5041 agagctaagc ccaacaagat ccgctacgta agacattcct aaacaaataa ctagcgatac
     5101 gatgataata gaagaatttg cgtacacttt ttctgcaaat gaaagtagga aaggcgcaat
     5161 ccatttgact aataagaaaa taaaaacaaa ataaattagc tgttcaatca aaacaagcgg
     5221 taaagaagca ttgctagaag cttcccccgt tagaaaagat aggctgaaac ttagaactaa
     5281 aacgactaaa atgtcatcta ccacagaagc acccaaaatt gttgatcctt cttttgtatt
     5341 cactacgttt agttctttta atacttctac tgaaatactg acagaagttg ccgcaagaat
     5401 aatcccaaag aaaatcgctt catttcccga aacttggaat gctttgcctg tcaaccagcc
     5461 aaaaacaacc gggaagatga tcccaagcaa agcaacgaac ataccaggtc ggaaatattt
     5521 tttcaaaaga gataaatcac tttccagacc agctaaaaac attaataaga tcacaccaat
     5581 ctccgaaaag tcatggacta gaatatcagg tttgacccaa ttcaatccag cttggcctag
     5641 caaaacacca acaagtaatt gtccaatcac tgctgggata cctaatctcc gcgagatatg
     5701 tgagccaatc gttgttgaaa tcaatatcag gcataaaata ccaataaatt ccattctcat
     5761 cattcctttg tataaaatat agcccgaact tagcataaca tacattattg aaaaaatagg
     5821 aaaatatact cacaaaaaaa ccaacaatcc aaaagatttt tttggattgt tggtttttat
     5881 acggtctatt ttaataacta aaaatgatta acgtatagtc atttttctta ttctgatgaa
     5941 attgataatc tcctggatgc ccatgaaaat caaaataaag ccgaatattt ggaatagaag
     6001 catcaaactt ttgaatggat tgaacaataa gacgaatccg ccaatcaaca acagaatact
     6061 actataaaca ctccaacttt tagcttttgt attcatgctg aagaacaact ggaacaagct
     6121 gttgatgata acagctaatc caagaagaat cggcaggata gaaacgattg caggtgcaaa
     6181 agcatagacg atcagagcta agatcaggaa aaaaagacca ctgaacagac cgatactcca
     6241 agaacctgtt ttccttttaa aacggtaagc ctcaaaaaga ttcaacaacc caagcaaaac
     6301 aagataggcg gtgatcagat aaccgataaa atgaaaaaca gcagaagggt ttagcacaat
     6361 cgcaatccca gaaataatat aaacagcagc tcttaataag gcgtattttt gaatattttg
     6421 tttaatcgtc tcaaacatat aagaaaacct cctttgactt attttaacgt gaactagtca
     6481 ttttaccaaa ttaaatgtta caaaatgagc gaaggagaag aatatctctg gaaaaatcaa
     6541 cttctgagga tcttttccat ttttttgcct tttgctagct catcgatcat tttatctaaa
     6601 taacgaattt ctttcatcaa gttatcctcg atgtcttcta ctcgtacacc acaaacaaca
     6661 cctgtaatca aggaacgtga aggattgggg acaggggctt ccgcaaaaaa ttgttcgaaa
     6721 gtcacttgtt tttgtagttg atattctagt tctgtttggg aataacccgt cagccaacaa
     6781 atgatttcat ctacttcctc ttttatacga tcttttctct ccgcttttgc tacatacata
     6841 ggataaacgc ttgcaaaact tgtcgtaaaa atacgatttt gcgccataat cagtcacctt
     6901 tctttttttc aaattattta ctacttatag tatataagaa aaagagaaga aaaagcgatt
     6961 attcaaaaaa acaaaaagga ttgtgacaaa attcttgtca ccatcctttt ttccgctctc
     7021 tgacttttta caatcttcat agctcaagtg cgcatcagat aatcgaatgc tcctaaagct
     7081 gcagtagctc ctgaacccat tgaaacaatg atttgtttgt atggactatc tgtacaatca
     7141 ccagctgcga aaacgccggg gatattcgtt gcgccatttt tctcggtcac tatttcattt
     7201 cttggattca tttcgatcgt tccatctagc caatccgtat tagggagtaa gccgactaga
     7261 atgaatactc ctgaaacagc tagtgtatgc atctccatcg tacggcgatc gatataggag
     7321 agagattcta cctgattcgt accgctgatt tctttcgtcg ctgcatgttt gataacagtg
     7381 acattcgcaa gagagactag tttatcctgt aagatttgat ctgcttttaa ttctggtaag
     7441 aattctagaa catatacatg ttttgctaac ccagccaaat cgattgctgc ttcaacacca
     7501 gaattaccac caccgataac agcaatctct tttcctttaa ataaagggcc gtcacagtgt
     7561 gggcaataag caattccttt atttttaaac tctttttcgc ctggtacatt gatagaacgc
     7621 cagtgcgcac ctgtcgcgat gatggcggtt tttgctttaa gtgatatacc gttagctaag
     7681 ccaatattaa taaaatgttg ctctttattt aaggaaacgg cctgttgatt catcataatg
     7741 tcgatagggt aactacgcac atgactttct acttgctgca tcaattttgg accttctgtg
     7801 taagaggtac caatcatatt ttcaatccct agtgtttcaa cgacttgtcc gccgaaagaa
     7861 tcagcgacaa gtcctgtacg gattccttta cgtgctgcat agatagctgc actactagct
     7921 gctggtccac cgccaattac taatacatca taaggttctt tttcaaacaa ctcttcttca
     7981 atcaaagggc cagtgatttt ttcaacaatt tgttcgatcg tcatgcgtcc gctactaaat
     8041 tcttctccat ttaaaaacac agttggcact gccatgattt ttttatcatc gatttctttt
     8101 tggaacatcc caccttcaat catcgtatga gtaatatttg gatttaatac agctaatata
     8161 ttcaatgcct gtacaacgtc ggggcaatta tgacaagtca agctaacata actgtcaaaa
     8221 tgcaatgttt gtgttatttt tttgatttta agactcaaag attcactgat tttcggtgcc
     8281 cgtccactaa tttgtaacat agccagtaaa aaagaggaaa attcatgacc gagggggata
     8341 ccagcaaaaa cgattccact ttctttgcca actggattga tagaaaaact aggtgtgcgc
     8401 atcgaatctc ttttttccca agagatttta ggagacattt ctgctatttc cttcgtaaat
     8461 tcagctagtt tttgactatt ttcactatta tccaaactaa cagaaaaaac aataggagat
     8521 tcaatcagtg ctaaatattg ttcgagattt tgtttagttt ctttgtctaa catatccacc
     8581 acctaaatct ttccaacaag atcaaaactt ggtttcaatg tttcgccact ttctttccat
     8641 tttgccggac aaacttcacc tgggtgggta cgtacatatt gagcagcgcg aatcttatca
     8701 atcagtgcac tagcgtcacg accgattcca tctgcattga tttccatagc ttgaacgatt
     8761 ccgtctggat caataataaa tgttcctcgc tgagctaatc cttgctcttc atccaaaaca
     8821 tcaaaaatac gagaaatctg atgagacgga tcaccgatca tcacgtattc gattttaccg
     8881 atagcatctg atgtatcatg ccatgctttg tgagtaaagt gggtatcggt agagcaagaa
     8941 aagacttcaa cacctaaaga tttcaatgta ctgtattgtt cttgtaagtc ttctaattca
     9001 gtaggacaaa caaaagtaaa atcagctgga tagaagcaaa gaatactcca gttccccata
     9061 agatcttttt cactgacttc taaaaattct ccttgatgat aagcatgtgc attaaattct
     9121 tcgatttttt tcccaatcaa tgacatagta aaacctccaa taataattat ttatttataa
     9181 ttattgtaat ttagttggag ttgcaaagtc aatggatatt ttgttattta ataaagatta
     9241 ctcaaaaacg aaggttaatt ataaaaatag aagaggtcgc aagaaaatgc tactgctgga
     9301 aatactcggg ataacgttga gtaacagttt cctgttcttt cagcatcatg tgtaagtgca
     9361 atcccgtttg aaatcgagct tcttctggtt ttttatcaac taaagccttg agtaaatcat
     9421 aatgttgttc catgatcgtt tcccatggaa gcccctgaat ggtcactcgt aaccagcgga
     9481 aacgttctaa atgggtatta tggtcatcta accagttcca aacttctttt cttcctgcaa
     9541 tttcgaaaca agcttcatga aataaattat ctgcacggaa aaaagcgcgt tcgtcattta
     9601 ttttgactgc tttttcttgt tcttctaaaa tcgtttctag cactattcgt gtttgtgtgt
     9661 ccaatttgga acaacattca atcatgattt gctcttccaa ctgttctcgg acaaagcgag
     9721 cattttctgc agaaacaaga ttaattttgg aaacatacgt accactttga ggtatcgtat
     9781 acacgagttc ttgctgacgt agttgaatca atgcttcgcg aatgggagtc cggccgatag
     9841 caagatgttc ctccagtgcc ttttcagata ttttccttcc aggttctaag tcagcataga
     9901 taatttgttt acgaatcgct tgataagctt gtgattgcaa gctgcttttc atctttattc
     9961 ctccaaaatc atttctatga acgtagtata acagaagagg tctgaaaaac aatctggtat
    10021 gtcacattaa ctagtaaatt tgtataaata gtaaaatttt ggttgacata gcgattttta
    10081 ttgtataaaa ttcacttgaa tacaaaaact aatatgttag tttaggaggg gaaaaatgat
    10141 tgatttaaac aactggagga actttatccc tgaattatct caaaaagaag tagaaatacc
    10201 tcattttgac cgtgcatcat taagagaaaa tggacaaaaa aatcctgtct ggatccattt
    10261 cggtggcgga aatctttatc gaggatttca tgcagaaatc gctcaacgtt tagcggatct
    10321 aggcgaattg tccactggtg tcattgtttg tgaaacattc gatgaagaaa taattgaaag
    10381 ggcttaccaa gattttgatg atttgattct tgaagtggtc atgccaagca gtggagagtt
    10441 cgtcaaacgt atattagctg cgacagctga cagtctgtat tgtcatccag attgttctga
    10501 acactttgat aagatgattc gacactttga aaatcctagt ttacaattag ttacttttgc
    10561 tattactgaa aaaggttatc gattgaaaga tacaaataat aaatatatag gaccgattgc
    10621 agtcgatata aaaaatggtc caaagaagcc atcacataca atgagtattc ttgctgcgct
    10681 actttataaa cgttttttat ctggagcttg gccgattgca cttgtctcta cagataattt
    10741 ttcacaaaac ggtagtcaat tacgtacttc tttgctaacg atcgcaaaag aatggcaaaa
    10801 ggaaggttat gtacaaaata catttatcca atacttatcg aatgaagaaa ttgttgcgtt
    10861 tccatggacg atgattgatc gtatcacccc gaatccttcc ttgtcagtta gcaaaatgtt
    10921 ggcaaaagag ggcttttcaa atatgtcgat tttccaaacc gcaaaaggaa ataatattgc
    10981 tccatttgtc aatacagagg acatccatta tttaatcatg gaagatcatt ttcccaatgg
    11041 acgtccgaaa ttcgaaaaag ctggggtatt cttgactgat cgtgcaacgg tagataaagc
    11101 agatacgatg aaggtgacga cttgccttaa tcctttgcat acgtctttag ctattatggg
    11161 ttgtttactg gggtatagga gcatagctga ggagatgaaa gatccagatt tatcgctgct
    11221 agtttctgaa atcgggtaca atgaaggcct cccagttgtg gagtctccag gtatcattga
    11281 tccaaagatc tttctggaag aagtaattac tgtgcgcttt tctaatccaa atattcctga
    11341 tatgcctcag cgaatcgcca cggatacctc tcaaaaggtg gcgattcgtt atggtgagac
    11401 gattaaaaaa tatatggaaa tagaagttaa agacccgcag acacttgtat tcataccatt
    11461 gactctcgct gcttggcttc gataccttat tggcattgat gatcaaggaa aaccattcat
    11521 cccgagtcca gacccactat tgacagactt tcagcataag ctttccgata ttcaactggg
    11581 tacgcaagat acatcacata ttcatgagaa aatggctcct attttgtcaa ataaagaaat
    11641 ttttggtgta gatctttacc aagcagagct tggcagaaaa atagaagaaa tgttcatcaa
    11701 gatggtagca ggaccgaatg ccgtacgaaa aacattacag gattacttac aagaacatgg
    11761 aggaaattta tcatggaaat gacttttaga tggtacggat cacaggaaga aaagattacc
    11821 ttagatgaaa tcagacagat tcctgcggtc aaagggattg taggcacatt gatggatata
    11881 ccagtaggag aggtatggcc cagggagagg atcaaagaac taaaagaaga aattgagtct
    11941 cgtgggttaa cactaaaagt aattgaaagc gtcaatgtgc atgatgccat taaaattgga
    12001 ttaccttccc gtgagcagta tatcgaaaac tataagcaga caatccgaaa cttggcagaa
    12061 tatggaatag aagtcatctg ctataatttt atgcctgtat ttgactgggt gaaatctgac
    12121 cttgactacc gcttagaaga cggctcatcc acgttagctt ttatatctga tgatattcca
    12181 gatgatccaa gagaaatcgt cgcgcgcatc gaacattcca gcaatgaatt tggattgccg
    12241 ggatgggaac ctgagcgctt agctcctatc aaatccttgc ttgaagccta tgcatcagta
    12301 gacgaagaaa aacttcgaga aaattttgcc tattttctcc aatcaatcat cccaacatgt
    12361 gaagaggtag gggtcaaaat ggctgtacat ccagatgatc cgccatactc gatttttgga
    12421 ttagctcgaa tcgtaaagaa tcgagaagat ttagattggt tgtgtcgcat agtagacagc
    12481 ccttataacg gaatcactct ttgtacaggg agtattgcag aagatccaga aaatgatgta
    12541 tatgggattt tagcagaatt ttgccaaaga gatcgaatcc catttgcaca tgttcgaaat
    12601 attaaatttt tagctaatcg agatttctat gaggcaccac ataaatccga atacggttca
    12661 ttagacatgt accgcatact aaaaacaatg tatgacaacg gatttaacgg atacatccgt
    12721 cctgatcatg gaagaatgat ctggaatgag acaggaagac caggatacgg cttatatgat
    12781 cgagcattag gtgctgttta tctaaatgga ttatgggaag caatcgaaaa aggaaacaaa
    12841 agtaaagata attagtgaaa aaaactgtaa ttttattatg taaataagaa ggatgttcaa
    12901 attcttgata cataaatccg gaggttgtta ataagcattc ggatttatgt atcttatttg
    12961 gtctaggaag taacttgatt ttcttttttc gattgatcca tgagtgcttc ttcttttaat
    13021 tcaaagatat attctgagat cctcataatc acttttttcc agtcttctgg tgtgatgatt
    13081 ccttttaaaa tatagatttt tttgatatga gaaggcaatt gtttcaaagg gcagtcgctg
    13141 ataacgatgt tgcagttctc cataaaatca ttttgtattt tgatcgattc atttgaaaaa
    13201 atttgttcta tcttaacgat cagatcttta ctgatataaa aatcacgcgt atagttcaca
    13261 ccaatcacaa ttcgcggctg ttttttgtag cgatcataca aatgtactag gtcttcaacc
    13321 atccattgca cattttcctt ttctaaaata actggtaaca agtgttgatt ttgttctctg
    13381 aaatccatat aaaattgttc aatggcggtt tggacttctt tcaatacgct atgttcttga
    13441 taaagcatga aagtaggaag gggcgtattt ggttgattga agttaagaaa tccattataa
    13501 agtttatttc gtgcgaatgc caatgagtaa agaatctttt ctttttcagg tataaacaaa
    13561 tcaaatacag tacaaaggga ctccaccatt tctcttgaat aaagaacatg aggaacgttt
    13621 gcttttttta aaagtttgta ttgctccaag cttgtttctt ccgattcgat tttcgtgata
    13681 gaaagtcgag ctaaaatatt cactaagatg cgttgttcgc gacaaattgg cagattaggt
    13741 cgcaaaatat ctacagagag atcaataaaa aagtcaatat attcatactc tgtgaattct
    13801 ttttgtacgt ctttcacaga agttgcagga aaattctctt tacatatttt taatagaaaa
    13861 tataatttat ctatcatacc gctatttagt cggtcaagta cttctttttt aaaaaaagta
    13921 ttcatcataa aagccactac ttgcggatct tcatcataaa attctgtagc agtacttgaa
    13981 aagatgttcc aataaatatc taatgctgca tattgaattt gtacctcttt tccagaaaaa
    14041 gactttctgc cattattaga aaaggagata gacaaaccgt actatgctaa aaatgcatta
    14101 atctttttca ttttcgtata caaataagag gcactatagt ttgtttgctg actgaaactg
    14161 ataatcgaat ttgtctgaca gctgaacaaa gaagtcaaga tctgataact gactgattcg
    14221 ttgcagtatc ttccaaatag acgataatag cagtaagccg gatcaattcc ttcaaaattc
    14281 aactttaaaa aaggtttttg cttatcaata tgcagttctg ttagattgag acgctcaaag
    14341 tcttgttcta attctttgat gtatagtaaa aaatttgatt cagacatatg gaaatgatct
    14401 gctaaatatt gtgtagtaat aaagaaacga tgttcagata ggaaaagaaa aatatctaat
    14461 tttgtattgc tttttttctt caagataaac gttttaatcg cttcttgagt ttgcatgaaa
    14521 gaggctcctt tttacttaaa ttgttaaaac ttgaaaatat tactgttttt ttcataaatt
    14581 tagacaaaaa aacagtaaac atataggtag aatctctact atcttatttc ttgcatacaa
    14641 aaaaatcaag gagtgagaga gttatgagat attcaaccat cttaatatca acatttgcag
    14701 ccttaagctt atcgcttact ataaatcaga cagtccacac agaggagaca gaaacagcca
    14761 aatggacaga aaccaagtgg gccaacgtca atcgaattac ctttcaggat gaattttcat
    14821 ccttcaattc aggagtcgca aaattaagaa aaggacaaac agcgaccatc aaagcaaaaa
    14881 ttgactattc gggagactta gcacctatac tacaatcagc tgttacattt gagaatgttg
    14941 atcccacttt gtcgattggc tatgacgaga acactttgac tatccaacac aagcaagcga
    15001 tatttacctt caccgttaca ttaaatcagg acttgttaaa accagcgctt tttactgtca
    15061 aggtatcaga tggactaccg aacgatcatc atttaacgcc gtatagtcag cggcagacaa
    15121 ttgagcaaga ttcagctatc gactcaggag gagatcttgt tgaagaacca acagataaac
    15181 cagaaaatga aaataagcca gaagtaccgc ctacagaaaa tccagacgga gaacagaaac
    15241 cggaaataga acctggagaa gaacccgata cagaaactca accagagcca gacaatgaat
    15301 caaaacctga aataacccct ggagaaaaac cagatgtaga cccagaagaa aaaccggatg
    15361 tgactccaga accagatacg gattctggaa accaaacagt accggaaaca aatccagaca
    15421 cagataatga aactgaaaat ccagaaaaac tagaggtaga tccagaagaa aaaccagatg
    15481 tgactccaga accagatacg gacgcaagag accaaggaat accagaaaaa ataaacaaaa
    15541 aaacaattca agaggatgga aaaaaagaga gtaaaaaatc aaatctagca attctaaaaa
    15601 taaatgaaga gcagctgaat aaaaaatcac gaatatttga tagcgcccaa tcagctgaaa
    15661 ccttaaaaag tagcaaggat acaacctttg ctagtcctga aacgaaaaat aaacagctac
    15721 caaaatccgg agagtcacaa aacaaagtta tactgtggag cggaatcatt ctattaagta
    15781 tagcaacaat gctttctgca aaaagattta aacaaaacag atctctctaa catttgaata
    15841 atgtctgtga acgcattttt caaataaaaa gaaatatcca gaaaacgacc ccaaaatacc
    15901 agattgaaat ctgatatttg gggtcgttca tactttatct ttcttcatat ttattttaag
    15961 ggatatctgt ttcttttgga agatggttcg ggatcttttt cactttcaat acatctaaaa
    16021 agaaggtaaa gatcggaatg ccgacaatca agccccaaac gccaaagaac cgttcgctaa
    16081 caagtaagac cacaaatgta taaaaaatcg gcagttctgt tttgctggac atcaacttcg
    16141 gattcagcac ataagactca attagatgga caatcaaaat cgtaatcagg atataaacaa
    16201 catctcggat gcctccttgg gaataagcta tgaaagatag aggaatacaa gaaatgataa
    16261 cacctgctac tgggatcaga cttaaaatga aaatcatgat cgctaaactc agcagctgat
    16321 gaaagccaaa tacagccaag gcgattgtag tgagcaacgt gtttaaaaga gcgataacaa
    16381 attgagcttc taataccaaa ccaaatgtat tgacaaactt atcggcaaaa tagtaaatat
    16441 cttgaaagaa ccaagaaaat tcacttttta aaaacaatct tgagaataca attgtttttt
    16501 tcttttcaat cataaaaaag aaacttaaga tgaatgacat agcaaacgcc actgccattt
    16561 ttcctatatc ctgtaaatag ccaagcgcaa tcgaagcgcc attttgaagc tgttcaaaaa
    16621 aatcgtttcg ctcaatataa gtatggatat attgtaaaac aggatctgca tcttttggct
    16681 gatgctcgta aaaatcaata acggactgga tcatatcaaa tgtttgatga ataagcatcg
    16741 ggatatactt tgtgaatgcc agatagataa ggtaaacgat cagtgtataa atcatgatag
    16801 ctaaaacacc tgtagggatt cggacaaatc gctgaatgat ttgaacaaga tgaatcatca
    16861 aataagtaaa gataaatgtg agcaaaatgg ttgtaatcat acttctaact aagaaaagaa
    16921 caaaaacgac aaataataaa acagtgagac ggcgtatttt ttcattcgcc aaaaaacgct
    16981 gataaacggt caaatcgaca acctccaaat aacaaaaaat aaacacgtta ttcattatat
    17041 gatggaatat gaaaataaga aagagaaaga gcaaaaagaa aaaccgctaa atgacgttat
    17101 cttatgagtt ggttttcctc ataaaatcgt tcatctagca gtttcagccc tttttctggt
    17161 cttgatgaaa attatctagc atttttcggg tcagatgttg cagtgtatcc aattcttcct
    17221 gttcaagtgg aagataagac ttgatctttt gtggaatacc ttctgcttgt ttttgcaacg
    17281 cagcaccttg aggagtcagc gtaacattta cgattctttc atcacccgtt tcacgctgac
    17341 gattaatata atttttttct gctaactttt ttagtaaggg agtcagcgta ccagaatcca
    17401 gacacaaatc atttcctagc tctttcactg aagtcttttt cttttcccat aaaactaaca
    17461 tcacaagata ctgggtatag gtaagattca gcggcttgag gagggaagtg tagtgtctaa
    17521 tgatttcctt agaggcggca tataaaggaa aacatacttg attaactaaa tatagatctt
    17581 ccatttttcc acctccgctg ttttactata gtgtatcata gcggatttac acaaaaaagt
    17641 ctattcacta actgctttca gagtaacttc tatatttcca gctgtagctt ttgaatacgg
    17701 acacacttta tgcgctgcct ctaataattc ttgcgtttct tccagagaaa gaccttcgat
    17761 agatccttca atctctacac ctaagacaaa gctcgtttca ctttctgaat aaagggaaac
    17821 ttgtgcactg acagtagaag ccccagagat atttttttgc tgtttgatca attctaatgc
    17881 actgttaaaa catgcactga aaccagctgc aaacaattgt tcagggttgg ttgcattttc
    17941 gacgcgttta ccaggagctg caatattcaa ttcgaatgta cgatcgggat tatgaacttc
    18001 tccagagcgt ccgcctgtgt tgatcatttt cgtagaatac atttttttca taaaattacc
    18061 acctttaatt atattttata caattgaatt ttacacaata taattgtgga aagcaaagaa
    18121 atggattggc ataagataaa taagagaaag cacatgaaac agacgcgctt tctcgaatag
    18181 caaacatata caaaaaaata tcagagacaa aaactaatct catcataaac attacatata
    18241 tttctcaaaa agttgttttc tttcttcaaa acttacttca cgatggacct gatgaagcat
    18301 ccataaataa ccaaaaggat ctctgaacat tgagttagtc acaccaaagt ccatcatttc
    18361 tgtcatttct tgaatctcta cacagccatt tcgtaatgct ttattatgtg tgtcctcgat
    18421 atcagagacg atgatattga accagatcgt atttggatgt tcagcagtag gagcagtcaa
    18481 accaagagac gtgttttcat ccagcatatg gaatctcgtg ccatacaagg taaaaatgac
    18541 ttcattattg cctctagggt aatctgttgc ctcaatcagt tgtatttcat caaagattgt
    18601 ttgatacagt tctagtgctt tcaaactatc atttaataca aaatctattt ccgttccttt
    18661 atgcataaag atctcctctc cttttcagtc gaacagttac tagattcttt tattatacca
    18721 gttaaagaaa acgcattcaa aaaaatagcg gatttactta ccaactcatt cgattggatg
    18781 ataagtaaat ccgcgtccca ttacttctgc tacgctgctg acaatgacaa aggcacttgg
    18841 atcgatttct tctatcaatg gtttcattcg ggaaaattca tgatcagtca caacgatcaa
    18901 taaaacctct tttgactttc gattataccc gccttttgcc tctagcaggg taaggccacg
    18961 tccaatctca gcacttaagc gaaggcggat gtcttccaaa taatgttcac tcatgaccat
    19021 aacagatttc tttcgattga tacctgtttc gatataagtc atgacaattg aagtgattgc
    19081 aatagataaa atagcataaa gaaactcttc tattccaaaa acaaacagat taagagaaac
    19141 aacgaatgcg tctgtaaaaa ataagccaat cgaagtattc aagtggaaat attttttaaa
    19201 aatgagggga gggatcgtcg taccgccgct agaagagttg tttttatata aaatcgcaac
    19261 tcctaaagca aaaatcgcac ttccaaaaat gacagacaac aaccgatctg aagtaatcat
    19321 tgtttctgga actattgcaa tgattaaagg aaaaaacaag cttccgaaaa gtactttaaa
    19381 aaagggtttt ttacctaaaa atagaagagc tagtaccaac ataacgatat tcaacactaa
    19441 aataactata gctcggtcaa atcctaaaac agcttccagt aatattccta gaccgctgac
    19501 acctccagca gcaatatgat gaggccctaa aaacatattg atagcgactc ctaatatgaa
    19561 gagggcaaaa ataatcttaa tgtatgtcca tataaattgt ctcgtttctt tcatttatcc
    19621 acttccttaa ttcattaata atactcttat cttattataa cttacttttc accttaagtt
    19681 aactttaatt aataaaaaaa ttaggctagt ctaaattatt atgtttcatt cttctagaat
    19741 atacttctac gttaaaaact gttaaaaaac ttttacttta gaaaattgga gaaattgatc
    19801 gcttaagata aattcaggac actaaatagt acaggaagta gattaaaact tttaaaagtt
    19861 ttaagtctat ctatttggca agtttaaaaa ataaaaattc ggtatcaaaa aactgttacc
    19921 gataacattt ttgaaagcgt tgtcttttgt ttctctatca cttatagtga tgacgtcata
    19981 ttaaaacaca aggaggaaat caatggatac agctgcaatt tatcatcgac cggaaagtga
    20041 atatgcattt ctatatacga atacaagatt tcgtattcgg cttcgtacta ggaaaaatga
    20101 tatcaaaaaa gtatatgtgc tttgtggaga tccatatacc ataacaactg aaaaatggta
    20161 tcaaaaacaa cgaccaatga aaaaatgtct aagtaccaat gttcatgatt attgggaaat
    20221 tgaagtgacg agcgaaacaa ggcgcttaca gtatgcgttc catgttgttg gggaagataa
    20281 cacggactgt ttttacggtg atcaaggaat ttttccttat cgagaaaatg tgattactga
    20341 accaaatttt tattttcgaa taccttattt tcatcaaatc gatcgattca ctattccaga
    20401 atgggtaaaa gaaacagtat ggtatcaaat ttttcccgaa agatttgcaa acggggacaa
    20461 agcaaatgac cccgaacaca ctttaccttg gggaagtaaa gatccagacc gagaagactt
    20521 cttcggggga gatttgcaag gtgtgaggga tcatttggat tacttgacgg accttggcgt
    20581 caatggcatc tatttttgtc ccatatttaa agccacatcc aatcataaat acgatacgat
    20641 tgattattat gaggtggatc ccgcttttgg cgataaaaaa cttttaaaaa acttgattga
    20701 tgaagcacat aaaagaggga tacgtatcat gttcgatgct gtattcaatc atatgggtgt
    20761 ccattcacca cagtggcaag acgttctcaa aaacggagaa aaatcgatct ataaagactg
    20821 gttccacatc cgttttttcc cagtagattc ttatcagatg actgacttac cagaaactgc
    20881 tgaaaatatc ccatatgata cttttgcttt tactccattc atgccgaaac taaatacagc
    20941 aaatccagaa gtgcaaaaat atttgctgga tatcgcaact tattggataa aagaattcga
    21001 tattgatgga tggcgtcttg atgttgctaa tgaagtcgat catcattttt ggaaaaaatt
    21061 cagagaagca gttacagaaa tcaaaccaga catctatatc cttggagaga tttggcattc
    21121 ctctcaagct tggctgcaag gggatgaatt ccatgcagtg atgaattatg cattcacaga
    21181 ttcgattaaa gattattttg caaaaaagaa aatcacagct tcacaaatgg tcagtggaat
    21241 gaatcatcag caaatgcttt acagagatca ggtgaacgaa ggaacattca acttattaga
    21301 ttctcatgat actgccagga tcttgaccct ttgccaaggt aacaaagaat tgatgaagtc
    21361 tgtgatggcc tttatgtttc ttcaaaaagg gtctccttgt atctattacg ggacagaaat
    21421 agggatgact ggtgaagatg acccggattg ccgcaaatgt atgatatggg agaaggagga
    21481 acaagatcta gagctattcg gttttataaa agaacttgtt tctctaagga aacagttgtc
    21541 taagattatt tctgaaggtt ctactcagtg gctaatagta tatgatcgtg aagataagct
    21601 ctatttcaca cgggaactag agggacagat catctatgtt tactttaatc agtcaaaaga
    21661 accttgggta gtagaacagg aaaatgaggt gattcttagt caaaattgtc agttgttaga
    21721 agacgggaaa gcagagatac gacaatacgg atttttgatc atgtgttgaa aactgttacc
    21781 ggtaacaaca agaaagcgtt tgcaaatgaa gatttgtgcc cattataata agggtgtaac
    21841 agcacgttga atacacagga ggaaatgaag atgaaaaaaa gaagtgtgaa acagttgggt
    21901 ttagggttaa tctctttagg aatcttaggc ggattagcgg cttgtggcaa tggtaactct
    21961 tcagcagaag gggaagccga tgaaggaaaa acgttaacag tttccgttga ttctggatat
    22021 aaagattata taaacgaaat aaaaaacact ttcgaaaaag aaaacgatgt gaaaatcaag
    22081 ctagtagaaa aggacatgtt cgatcagttg gaatcattag cgttagatgg gcctgctgga
    22141 aagggacctg acgttatgat ggcagcatat gaccggatcg gtgcgttagg acagcaagga
    22201 catctggcgg aagtaaaact gggaaatgaa gcagcttacg atgaaacaga caaagcccaa
    22261 gtgacttatg atggaaaaat ctacggagaa cctgcagtaa tcgagaccct tgttttgtac
    22321 tataacaaag atttagtcga tacagcacca tcaacgttta aagatttgga gaatctatcc
    22381 aaagacagtc gttttgcttt tgagtcagaa gcaggaaaaa atacaggatt tttagctaaa
    22441 tggactgact tttattattc ctatggttta attgcaggtt acggtggcta tgtatttggt
    22501 gatgatggca caaatccttc agatatcggt ttgaataatg caggtgctgt agaaggaatc
    22561 tcttatgcaa cagattggtt caaaaatgtt tggccaaagg gaatgcaaga cataaaaagt
    22621 gcaggagact tcgccagcca gcaattcatg tccaataaaa ctgcagcaat cattgacggt
    22681 ccatggcaag cgcaaacata caaagaaaac aatatcaatt atggtgtagc caaaattcct
    22741 actctaaata atggacagcc ttatcaaccg tttggcggag gaaaaggatg ggtagtcagc
    22801 aattatgcta aaaacaaaga cctctctcaa aaatggcttg attatgtaac gaatcaggaa
    22861 aatcaagaaa aattctttga aatggtaaat gaaatccctg caaaccaaca agctagggaa
    22921 acagcaaagg ctaaaaacga tgaattaaca acagctgtga ttgaacaata tgaaacagct
    22981 caagcgatgc caaacatccc agaaatgggc gaagtttgga caggtgcaga aaacttgatg
    23041 ttcgatgcag catcaggttc aaaaacacca aaagaatccg cagatgaagc agtaaaaaca
    23101 atcagtgaag ccatagaaca aaaatactga ttaaaatgat aaccgtccat ttttataaaa
    23161 aatggaacag gtgcttcaga taaaagtggc aagagcgaga tggaaaatta tcttgctctt
    23221 gttgccatta tcttaaaagc agtttgcggt ttagcaagtt tagagggggc attcagatgc
    23281 agcaagataa aacaagcaat atcaacgaaa aaaaaatcac gctttgttca cttattccag
    23341 ggatggggca gtttgtaaat ggccaagctt ttaaaggtat cgtttttttc gccatattcg
    23401 tcctattcat tggtcagatg cttctgggcg gatttcatgc attggtagga ttggttactt
    23461 tgggaagtat accaatggaa gatcattcat tatttatttt gattgaaggc actttgcaat
    23521 tcatcattac aatcattttc ttgatttttt acggtttgaa tcttttagac gcttatcgag
    23581 tagcgaagat gcgtcaagag ggcaaaaaaa tcaatcggac gatgaaggaa gtcatacata
    23641 atataggtga tgaaggtttt ccttatctat tgacacttcc cgcttatctt ctcatgattt
    23701 ttacgatcat tttcccagtc ctagttacat tgttcactgc atttactaat tatgacttca
    23761 atcatattcc accagccagt ttaattgatt gggtaggggc taaaaacttt tttagtatct
    23821 tctttttaag ctcatatcgt aatacttttt ttgcagtctt ttcgtggacc gtgatttgga
    23881 ctttatgtgc aacaactttg caaatcaccc ttggtattct cacagcggtt gttgctcacc
    23941 agaaatttat taaattcaaa cgtattttcg gtatcatttt cttattacca tgggcggtcc
    24001 cagcttttat tactatcatg agtttttcga atatattcaa cgatagcgta ggtgccatca
    24061 atactcaagt gattcctttc ataaaccagc ttccttttgt agatatcagt gcaatctctt
    24121 ggaaaacgga cccgaactgg acgaaagtag caattatctt agtgcagggc tggttaggat
    24181 ttccctatat ctatgtgatg gttacaggaa tcttacaatc gatttcagaa gacttatatg
    24241 aagcagcaaa aatcgacgga gcaaatgcat ggcagcgctt ttcaaacatc acgcttcctg
    24301 ctgtcttcct agtggcagcg ccaacattta ttacacagta tacggggaat ttcaataatt
    24361 tctccatgat ctatctgttt aacaatggtg gtcccggaag cgtaggcgga ggagctggtt
    24421 ctactgatat tcttatttcc tggatctata aacttacgac tggaacttct ccgcagtatt
    24481 ctatggcttc agcaatcacg ttgattatct ccgtcatcgt tatctgtgtg tcattgctgg
    24541 tatttaaaaa gacaaatgcg ttcaatatgg gggattaaca atgaaaaaat ttcattcgag
    24601 tgctcgattc caaaaacgac tttctcactt ttttacttac ttttttcttt taatactgtc
    24661 agtcattatc atctatccgc ttttgatcac agcaagtact gccttcaagt caggaaatat
    24721 tgcagctttt agtttagatc tttcaagtga ctggacttta ggaaacttta ctcggctatt
    24781 cagtgaaaca ctttacggga cttggtataa aaatacattg atcatttctg tcctaacaat
    24841 gatcgtacaa gtgactgtag taactttggc gggctatact tatagtcgct atcgttttat
    24901 cggccgcaaa tcaagtttga tgttcttttt agtgattcag atggtaccga ctatggcagc
    24961 attgactgcc ttttatgtca tggctttctt attagaagcg ctcgataagt actggttctt
    25021 gacaatgatc tatataggcg gtggaattcc aatgaatact tggctgatga aaggatactt
    25081 tgacactgta ccaatagatt tagatgaatc tgctaaactt gatggggcag ggcatttcag
    25141 gatttttttc cagattgtat taccgcttgt caaaccgatg ctcgccgttc aagcattatg
    25201 ggcttttatg ggaccttttg gggatttcat gttagctaag ttcctgcttc gaacacctga
    25261 aaatcagact gttgctgtcg gcttacaaac atttatttct gatgtgacaa accaaagagt
    25321 ctctcttttt gctgctgggg caattttgat tgcactaccg atttgtctat tattcttctt
    25381 tttacaaaag aactttgtta ccggcctgac atctggcgga acaaaaggat aggagcagag
    25441 tatcatgcaa acatttccgt taaactattt ttccgcatta cgtacgccaa ctcaagtgtt
    25501 tgcgggacgc aaactattat catggcctaa atttttcttg atatttgtat ttttagtttc
    25561 cttgatggtc atgcctgtga ctctttttta tgccaatcag atccaagcga ttccattaga
    25621 acaatttcta tccgttcatt cgctgatcga tgaacaagga acccaaaaat tttctgaact
    25681 tgaattatct gaaactgggt tacaagcatc gcaacaaacg atagctgtga cccctgaaat
    25741 tttggtaggt gtgtctttat ctgagaaaca acaatccgat catggaactt ttattgattt
    25801 tgagaaggaa caatgggtga tccaacaaaa ggataaatct ggtatacgcc gctatacgat
    25861 gaattatagc ccatctttcc aaccagattc tgtacgtacg cccgaagatt tccaacgatt
    25921 tttagaaaga gaattttatg ccagtaatcg tccaacaatc attttatctt atagtttaag
    25981 tcttggtctt gtcctatttg tcatgacgag tctcatttta ttcggggctt cctttttctt
    26041 gtggatgaca agaaaaagcc aactttcatc cattcataca ttcaaagaga gtgcaaattt
    26101 gatgctgaat gttatgggaa taggttccat ggcagcagca gttgtagggt ttatccactt
    26161 tgactttatc ttgatgctaa gtgtccaaac aacgataact gtcttgctgc ttttatgggt
    26221 ttttgtcaaa acaagattca aagataagcg tgtcgaatga caagtttaag cgatttatgc
    26281 gacaatgaat gtgaaaaaaa tgaaaaataa agtagttttt gaaaaatttc tgaaaaaatt
    26341 aaacttctta gttgattctt ttctttttta gtattaacgt aatagggtag tttttttaag
    26401 gaggaaaatc atgttagaga aaactttata caatcaatta ttttcacgtt ctttctcgct
    26461 ccctgtcgaa gtaaattatt gggacggtac aacgaaacaa tacggagata cggacactcc
    26521 tccccaaatc aaaatcaata tccatgaaga aatcccaatg aaagagatta caagtaacgc
    26581 ttctttggca ctaggagaag cctatatgga tcatcgaatc gagatcgagg gaagtatcca
    26641 agcattgatc aacgatgctt atcagcaagc aaacagcttt ttacgaggaa aagattatct
    26701 aaaatggctg ccaaagaaag aaaaacatac gaaacaaaaa aacaaagaag atatccatgc
    26761 gcattatgac ttaggtaacg acttttataa actttggcta gatccaactt tgacttattc
    26821 ttgtgcttat ttcaatacac ctgaagatac gttggaacaa gcacaaatca ataaagtcca
    26881 tcatatctta gacaaactct ttatcaaaga aggcgaaacg ttgcttgata tcggttgtgg
    26941 ctggggaaca ttgatgttca ctgctaccaa agagtataat gtcaaggcaa ctgggatcac
    27001 actaagtgaa gaacaatacg atttgattac caaaagaata aaagaagaac atttagaaga
    27061 taaatgccgt gtattattaa tggattaccg cgaactaaaa ggcgaaacgt ttgatcatat
    27121 cacaagtgta ggtatgttcg aacatgtagg ttctgaaaat cttgaaggtt atttcaaagt
    27181 ggtaaaagat ttattgaaac caaaaggaac agctttgatc catggaatca gccgacaaca
    27241 aggcggtgcg acaaatgctt ggatcaataa atatattttc cctggcggtt atgtccctgg
    27301 tgttactgaa ttagttgata atattacaaa aaacgatttg caactgattg atttggaaag
    27361 cttacgtcgt gactaccaat tgacattaga acattggaca aagaattttc atcaagtcca
    27421 agatcaagtc acgaaagaaa tgggtgattc cttctatcga atgtgggatc tatatttaca
    27481 agcttgtgca gcttcatttg aatcaagtaa tatcgatgtg attcaatatt tgcttgttca
    27541 acctggaaac aatgacatcc caatgcatcg tgcataattg aggcaaaagt actgtttaaa
    27601 gactatttaa aaagaagcta agttttatga tgctccagaa gtgaactttt ttgtctaact
    27661 tctaggattg tctctaaagc ttagtttctt tttctttata ttttcataat aaaagtttgg
    27721 ttctttaatt tttaaacatg acagaaaatt ttttcttcgt tatacttgta atagaatact
    27781 attttataac aaaggggatc gaatcaatga aaaaaactgt tatttatact agtttatttg
    27841 cagctttgtt tttagctgct tgtcaatcaa ataccaatca aggcacatct gggaatacgt
    27901 cttccagtag cgaacagcgc tcttcacagg tatcggactc ctcaacaaaa gcaagcacat
    27961 cttcgtctag ccagacaaaa agttctagtg aagcaagtac tcagtcagat atcaaagtgt
    28021 ctgcagctcg tgcgatcgaa ttgttccaag ataaataccc agatactgca attactagtt
    28081 tggaattaga tagtgattgg ggatcttact tctacaagat agaaggtgtc gatgatcaaa
    28141 aagaatacga agcaagaatt gatgctcaaa atgaaaaaat ggagacagag aatccagaaa
    28201 ctctagatcg agatgaacaa aatggtcaaa agaaaaaaga agatggtctg gatgttgata
    28261 aattgatcac gattgaggaa gcagcaaaaa tcgccgtcga caaagtagga agcggtacgg
    28321 caacagattg ggatttggat aaagatttag gtactactta ttgggaagtc aaagtaaaag
    28381 acggcaatca aagtacatca gtcaagatca atagccaaac aggcgaagta ctgtcaactg
    28441 aacaagatga ttaagaattt cttcggaaaa caagagttga tttttctttt agtcatgata
    28501 cactagacaa aataacttgt ttgaaggagc gatttacttg agtttagaaa cgacctttgc
    28561 caatcatatc tttgccaatc ctttgatgaa tgcatctggt gtccactgca tgactaccca
    28621 ggaattagat gaactggctc attcagaagc aggagcattt atcactaaga gctgtacgat
    28681 caatgaacga aaaggaaatc ccgaaccacg ctattttgat gttcctttag ggagtatcaa
    28741 ttctatggga cttccaaact taggtttttc ttattatcta gagtatgctc ttgcgtatga
    28801 aaaagtgcag gaaaaccaaa atcaaccgtt atttttttcc attgccggga tgagtgtcca
    28861 ggaaaacttg gaaatgcttg agaaaataga aaaaagtggg tttaatggga tcactgaatt
    28921 aaatttatct tgtcccaatg tgcctggaaa accacaacta gcttacgatt ttgaggccac
    28981 ttatgaaaca ctgaaagaag tattcagcat cttttctaag ccgctaggaa tcaaattgcc
    29041 tccttatttt gattttgctc attttgatca gatggctgat atcttgaatc agtttccatt
    29101 gacctatgtc aatgctatca acagtgtggg caatggtttg tatatcgata cagaacaaga
    29161 agcagtagtc atcaaaccaa aagaagggtt cggcggcatt ggcggagagt atatcaagcc
    29221 gactgctttg gcgaatgttc gcgcttttta tacacgtttg aaaccagaaa tccaaatcat
    29281 tggtacaggt gggattcgga caggacaaga tgcatttgaa catctgctat gtggcgcaag
    29341 catgttacag attggtacag agcttcataa agaagggccg gaaatttttt ctagaatcat
    29401 aaaagaatta acgcagatta tgtcagaaaa aggatatacg tcaattgacg aatttaaagg
    29461 caaattacgc accatttcat agcaaagaga cgaaggtcat gattgatctt cgtctttttt
    29521 tataaaaaac actcgtctgt tcattcctgt gcaagcgtac tcattgtatg ttagacgaac
    29581 ttgtgggaaa ataacagagg caaaaaggag gataatatga atgatgcaac agaattagca
    29641 aaagagctta aagaaaaaga atttagtttt caagaactag ttacggatat acagaaaaaa
    29701 ataaagaagc aaaatcatga attgaatgcg tttgtcgcgc ttgaaccatt cgagagtaat
    29761 tcccacgagc tattgattcc ccagtcagaa aaagggccat tggctggtat cccatttcct
    29821 ttaaaaatgc tcggtcaaga aaaaaaggga tggctagcta cttcaggatc tcgtttattt
    29881 gaaacacacc gagcttctca tacatctaat tatgtgcggc aagcagaagc gataggtcta
    29941 gttccttttg gacaaaccaa tgcgccagag tttggtttca aaaatattac agacccagtc
    30001 atctatggcc cagcaagaaa cccttggaac cttgaatgta caccgggcgg atcaagtgga
    30061 ggagctgctg ctgcagtttc atcaggaatc gttcctttag ctggtgctag tgatggcggt
    30121 ggttctatta gaatacccgc ttcgttttgc ggattgattg ggctgaaacc ttcgcgaggg
    30181 acgatgccag ttggtccata tgcttggaga ggatggcaag gagctgctat tgatttcggc
    30241 ttgacagttt ctatgagaga cacggaggct ttattttatg ggatgagaag tatccattcc
    30301 ggtgcgccat atcaagtatc gccagttgag tggcaaacac atccaagaaa aaaacggttg
    30361 aagatagctt tttgtgttga ttcaccgatc cactcacaga ttagtgaaga ggcagtactt
    30421 gctgtaaaaa aagctgtaga tttccttgga aaagaagggc atgatttgac agagattcct
    30481 tacccactag atggtcgtaa actgatcacg tcttattatc agatgaatgg cgcagaaaca
    30541 gcagcaatga tccgctcgat tgaaacaggc atccagcgag ctgtcagaaa agaagaactt
    30601 gaacccattt cttggacttt gttccaatat ggtcagaagc tctctgccgc tacttacatc
    30661 gattctctgc atgtatggga tgaagcagca attgtcatgg aaaacctaca tcaagaatac
    30721 gatctattcc tttcaccaac gactgcattt accgccccta aaatcagcga agatttgcaa
    30781 agtgatgcga taagagcaca gatggaacac atcagtgatt gttctgaagt agaaggttta
    30841 gacgttgttt cttccatgtt tgaggagagc ttgaagatca caccttatac acagttggct
    30901 aatctgactg gacaaccagc aatcagcttg cctactcatt tgacaaaaga aaacatgcct
    30961 ctaggaatcc aatttatggc tgccagagga agagaagatt tgctatttca aatcggctat
    31021 ctatttgaag caaatcatca gttccattta ccaaatccat ggaaatagca gaaacaagga
    31081 gaagaagaat gaagaaaaaa agattgtttg ctctaataac tagtgccttt tttattttaa
    31141 gcgcttgcgg tacagctgcc gctaatcagg aagataacag ctggagtaat gtagaaaaaa
    31201 agaaagaatt agtagtagcg acttctggca cactgtttcc cgcatcttat tataatgatc
    31261 aaaatgaatt agttggttat gatgtagatt tggcaaaaga ggtggctaaa cggttaaatg
    31321 tcaaaatctc ctttaaagaa tacaacgtgg atggacaaat cacttctgtt tcaaaaagag
    31381 aatcagattt tgctgcgaac gattttggta tcacaaaaga aagagaaaaa aaattctccc
    31441 tttcagagac tttcaaatat tcatttgaca gtatgatcgt acgaaaaaaa gatgattcag
    31501 gaatccactc acttgaagat cttaaaggga aaaaagcagc tggcgaaccc aatactagct
    31561 atatgcgttt agcggaaaaa cacggagcta aattagtcac atatgacaat gccacgaatg
    31621 atcagtattt aagcgatgta gcaaatggtc gaacagatgt tattctaaat gattattatc
    31681 tgcagaaaat ggccgtatct gctttaccag atatccctgt aaaaatactg gagaacgttt
    31741 atttcaatga aaacgaaagt ggttttttat tgaaagaagg aaatgatgct ctgaaaaaga
    31801 aaatcgatgg cgtattaaaa gagatgaaag aagatgggac gacaaagaaa atatctgaaa
    31861 aatattttca aactgatgtt tcagtcaaac cgaaagaaac gatggctgag tcggtgagtg
    31921 agtgatgtac tttcccctaa ttgatttttc cctcatgtgg acttcacttc ctttcgtact
    31981 gcaaggcttg gtatacacat tagggattgg cttcgtgtcg tttcttttag gaaatttggt
    32041 agggttgctt ttgacagttt taggtctttt ggattggctg cctctgaatg tatttattcg
    32101 tttttattta tcgttcttta gaggcattcc cgcactagtc ctgctgtttc tgttatattt
    32161 cgggttacct tatcaattaa gtgccttaac ggcttcagtc atctgcttta cgattaccag
    32221 cagtgcattt attggtgaaa tctatcgagg atcactagca ggcgtttctt caggtcagtg
    32281 ggacgctggt ttagcttctg gtttttcttt ctttggggta ttgaaatata tcgttttgcc
    32341 gcaagctttt cgaatctcag taccggcact cagtaacgta gctatggatc tagtgaaagg
    32401 tacttcgtta gcagcaacaa tcacagtgcc agaaatattt caaaaagcaa agatcatagg
    32461 cggtcggaca ttcgattatt tttctctcta tattttagtt gcactgattt attgggtcat
    32521 ttgtatcggg atcgaaaaag tgcaacagta tttagaaaaa cgttctatcc atcagtcaat
    32581 ctgaaaaata gaaatggtgc tagcgtggct ggcaccattt ctattttttg tttcttgtat
    32641 attctgccta gtattttaaa accatttcat gtaaaatgta aatcgaaaat atgttcggag
    32701 gtagaaaaca tgaagacccc gctaaccatc gaaatagaat cctcgttata ccaatattgc
    32761 atcgagcaag gcggcttggt tgtagaagaa gtcacgatgc ctgatgacca aggaattgta
    32821 gatacgctta gctgcttgac aaaatttgat ggaagccgag aatggcgatg ctatgagctg
    32881 aaagtgacaa aagcagattt tcgctctaca gccaaactgt catttgttgg acattataat
    32941 tattttgttt taccaagtaa actatatgaa gaagtaaaaa ctgaaatccc tacaggaata
    33001 ggtgttttag tgtatcgtcc gtacacggtt gaagaggaga tgcctgcctc tggtacattt
    33061 ctgattgcga aaaaagcagc aaagaaagag cttgctgtac cggaagaagc tttgacaaac
    33121 cgcttcatgg cttctttatt cagggaagta agaaaagcaa agcaaatgga gcgaggcgta
    33181 aaatattttc caaaagatca attgtacaaa gaattgaaaa gacgttcaga aaatcaagat
    33241 ccattttcta acgaacgata ttatgaacgg tttttagagg aaatagctag tcaggcaatc
    33301 aatgagttgc aagaagaagt tatcgcgctt cagcatgatt atgaatattt gaggcaacaa
    33361 cagcagatcc gtcggttgcc tacagagcca ttggagtgaa aaatatgtac tatccttatt
    33421 taagaggaaa acaatttgac ctattagcat taaaagaagc actgtcgcga ggattgctct
    33481 ccaataaaat ccaaccagtg atcgaaccag tacgtgattc cgctacgctt aaaaatgtga
    33541 tcgaattatt tcaaaaaaag caccatccaa tcgctgtgat ccaaaatccc caagtaggac
    33601 aattcaaatt attcgatcag catgtgcaca gttggacagt caaagaaaat agctctgtcg
    33661 tccctgcaca gatcttaacg ccagaaaatc tcagtgaagt actggattca cctccagcgt
    33721 ttcttgtttt cgatggacag cactacccaa aagatacaga ggtgtggaaa cagttggcag
    33781 gagttgattc gaaatttttg ataccagata ctagtcgctt tcgcatctgg ctgcccgaaa
    33841 acaaaattgt gatccgtgac tcttttcaaa cgagaaagca tgttgaaagt tatgcagata
    33901 aaacagatga cttttttagt gatgactatc tttttttcca cgcagacgga tatattggat
    33961 tttctgattt cacgatcgaa ggtagccgtt atttcgataa agggttccct agccgagcgc
    34021 ttgctatcca tctaacgtat attgatgctt acggcaatat acgcgtgaaa cattttgtct
    34081 ctgacagtaa cgactctgct aaagaccaag cattgaagtt tcttgaagca ggagataaaa
    34141 tgaaaaaatg gatcatgcgt caccatcatc aactcttgat tacttcagga atgattgaat
    34201 tattggctct ttaccagcaa caaaaattcc ctggactggg agtgttgaaa aagtggtcac
    34261 ttatgcatca tttggaactt attagtcacc tgctagatca tccaaaagat tggcttaaat
    34321 attattcaaa agttcaggaa ctatatgaag tgatccaatg attagataat gtaaatgaaa
    34381 aaggaataga aaagaggaaa ctggatgtat attcggcaaa caaggaaaga agaagtagaa
    34441 gaagtggtag aaatcattaa tgccgcagcg gatttcattg cttcgcaagg ctctcctcaa
    34501 tggcaaaacg ggcagaaacc aacaaaagaa acgattttga acgatattga aagaaaagaa
    34561 agctatgtat taatagtgga agatcaaatt gctggaacag ctgcattagt ctcaggcgtg
    34621 gatcccgttt acacaaaaat agatggtagt tggaaagaat caactggtcc ttatttatcg
    34681 attcatcgtg tcgctttagc agaaagtact cgaggaaaaa aactagcaaa accgttctta
    34741 gaacatttgt tgacgattgc agccagcaaa aatatccaag atatccgtat cgatacttat
    34801 ccagctaatg aaccgatgga aaagaccatc tattctgctg gattcactta tcaagggatg
    34861 attgaatttc cttttgaaca cggcgaaaga aaagcatatc aaataatcat caattgacta
    34921 taaaatcatc tgagcaaaga taaagacatt aacactactg aaaaaaacaa tcatatgata
    34981 gccaagaggt tgtaacagaa gtggccagct tcaaaaaaca agaaagaatt tccgaaaatg
    35041 cttctcaaat ttttgtggaa ttttagcgta ttttacgaaa tagctgcttt ctgaaactcc
    35101 cttcatcgag aggtcgtgac atagcttttg tcacgatctc tcggcttatc ctacgagtat
    35161 tattcgatac atttatatca ataatccttt ttttatttta tctgcagctt cttggaacgc
    35221 tccatcgttt gacacgacta aaagtgaggg gatagctttc aaacgtttca actgtgctaa
    35281 atcttctaca taaaccgcca gtctggctga aatctgttca gatgaatctc cacgtttttt
    35341 caggcgcgat tgcaatgttt ctttagagat cgtaataaag ataggacgga ctaccgtccc
    35401 gaaatactca tataaatgcc aaaaaccatt tgctgtcaaa acggcgtaac aatttcctac
    35461 tgtcagtttc tttttaattt ctgctttact ggaaccatat ttatgttgag catattggtc
    35521 ccactctgca aactcatttc ccttgatcat ctgattgaat gtattgtcag aaacaaaata
    35581 ataatcctct tgatcttttt ccccaggtct tggtgatcga ttcgtgtaag taatgatctt
    35641 ttgttccggc tgaaaaatag cttctgcgag tgaagttttt ccagaaccag aaggaccgat
    35701 aaatacatag caataattca ttgtcatttc tcctttgatg attgaacaag tgaaaaagct
    35761 ttggaaaata tttccaaagc ttttaaaagt aactttgctt ttttgtattt tataccagca
    35821 agccagtcac aatagcactt aaaaagctga ctaaagtcgc tgttagcaaa attttcaatc
    35881 cgtgttttgc gacaagattt cctttttctt catttaatcc cttcatcgca ccagaaatga
    35941 tcccgataga agagaagttg gcaaatgaaa cgaggaagac agatataatt gctgttgtac
    36001 gctcagtaaa atggaaatta ccatttgcta attctgtcat agcaacaaat tcatttgtaa
    36061 ccagtttagt cgccatgata ctaccggcat ccactgcttc tttccatggg attccgctga
    36121 tgaaggcaag aggggcaaag atgtaaccta ggatctcttg gaaagtgatg ccgaatatcc
    36181 cgtggaagat agcattgatc atggcaatca aagcaacaaa accgataagc atagcagcaa
    36241 ccgtgatcgc cacatggaaa ccatcaagaa tgtattctcc caacatttgg aaaaatgtct
    36301 gttctttgtt ttcttcaatg atcagctcat cttctttttc ttctaataca tagggattta
    36361 caattgaagc cagaatataa ccgccaaata agttcaaaac aagggcagtg atcacatatt
    36421 tagaatcgat cagtgccata taagatccga cgatcgacat agaaactgta gacatagccg
    36481 atacactcat ggtgaacaaa cgtttctcag ataggaaggg taattccttt tttatggaaa
    36541 taaaaacttc cgattgtcct aaaatagcag aagctactcc attataagat tccaatttgc
    36601 ccatcccgtt gattttactt aatcctagac caacagcttt cataaataat ggaagaatct
    36661 tgatatagcg tagaataccg ataatagcag aaataaatac aattggcatc aatacggaaa
    36721 gaaagaaagg tgcacttcct ttatttgcta caccggcaaa aacaaagtca acaccttgcc
    36781 cagcaaaagc aagaagatga tcaaacattt ttgccaatac agaaacaacc gctgtaccaa
    36841 aagttgtacg taataatacg aaaccaagaa caagctgtaa aacgaacatc acaattagtg
    36901 gtttcaattt gattgctttt ttgttactgc tgatcaaaaa agcaagccct aatgtgacaa
    36961 ataaaccaat aatacctaat aaataactca aataatcact cctattccaa attccgtttt
    37021 attttacctg ttttgcaaat aaaatactaa tctttagtga aattttcatt acttttttag
    37081 agtgaaacgc attcattttc cattagaaaa taaagcgaac aaaataaaag gtttaaaaag
    37141 tgtaacgtct tagtaaagtc tataaaaaaa cgaaactatc gattaagcta atagtttcgt
    37201 tttcaacatt tataagtaaa ctttttctat tggtaatata gattttcgga agggtagaca
    37261 ggtgcgcagg taatatcgat tcctagtttt ttcaatgttt gttcatcatt tttattgacc
    37321 atcacagtag agtgggcttg tgctccctca agttcagcta gtttatcata agcaacttga
    37381 gcgattggat tcgttactgc actgattgat aaagcgatca agacttcatt gatgcttagt
    37441 gcatccaaag aggaatgaag tgtctttgat ttcaatcctt gtattgtttc aagaactaat
    37501 ggtgacaata aaggaatgtc atctgcgata tgggctaaat gttttactgc atttaagata
    37561 actgccgctg acgcatccat caatgaactt gtacgcccgg taatgatgac ttcatctggt
    37621 aattcgattg caatcacagc agaaggatct tttgtaggac tttgcgcttg aatcttatct
    37681 gcatagtcat gggcagggac taccacgttt cggtcttcag gacgtaaacc gacttcttcc
    37741 ataatcagtt tgattcgatt aactgcttct tcgtcgatca atcctttttt gaagtcgcat
    37801 tctgtttgga aataacgacg gatgatttct tgtttagagg cttcttggac aacggtatca
    37861 tccacgatcc caaatcctac acgattcaca cccatgtcag taggagattg ataaacagat
    37921 tctttaccag taattttttc aataatacgt ttgattacag gaaatgtctc aacatcccga
    37981 ttatagttca cagctacttt attgtaggca tcaaagtgga aggaatcgat catattgaca
    38041 tcttttagat cgactgtggc tgcttcataa gcaatgttca gcggatgttt caacggaaca
    38101 ttccatactg ggaaagtttc gaacttggag taaccagctg catgtccttt ttggctttca
    38161 tgatacagtt ggttcaagca agtagcgagc tttccacttc ctgggccagg agctgtcact
    38221 acaacgattg gtttagttgt ttcaatatac tcattttttc caaaaccatc ttctgagacg
    38281 attttttcta cgttgactgg atagtcttcg atttctgagt gtttatagac cttgatgttt
    38341 cgtctttcta gtttattgat aaagactttc gtggcaggct gtccactata gcgagtgata
    38401 acgacactgt tgattaataa gccataacca cgtaattcat cgattaaccg caagatatcc
    38461 atatcataag taatcccata atcaccacgg atcttattac gttcaatatc tccagcatag
    38521 acgcaaatca gtatttcagc ttgatctttc aatttttgca gcaatttgat tttcgcatct
    38581 tcttcaaatc ctggaagtac acgtttcgca tgcttgtcgt caactaactt cccaccaaat
    38641 tctaaatata atttgtcata gtggttcact cgttccaaaa tatacgctga ctgctcttca
    38701 atatattttt ctgaatcaaa accaattttt ttcactaggt tccctcctta agtactaaag
    38761 cattttttca tttttttgag gattcgtcaa ctggaagagc gaaattcctc aaaaaaacaa
    38821 agaaaatggt ttaaaccaac ttgtctagat agttattcat tggtatgata gtttttgata
    38881 aggaaggtgc tacatgaaaa aaagacagaa aaagaaaaac gcatataaac actatatccg
    38941 ttcgattttt accggttatg aaagaatgct tgaagatccc gaattggaac aattgacgtt
    39001 tacttatcta aacgaagaaa cgcagctaac tcgtgatgag catcagcgca ttcatttcac
    39061 aacaagagat ctgcctagta aataaaaaat aagataaagg tcatcggatt cgattctgtc
    39121 ctatcagatg gaaagactag ttgatcgaaa aaaaggctgt accaaatttt ggtgcagcct
    39181 gttttatgga gcattgcccg ctctttttta taacaatttt ttacagaaaa tggtactatt
    39241 gaagtattaa taagtgaaaa acagatgagt aagaggaggc aagtagtatg aaagagccag
    39301 atacaaatgc aaattatgag ccagacgaac tagaggagac gaacgcggat caggatacag
    39361 gacaagaatc gtcttctttt tacgatatca aagaaaaagc aagcatgaat ctttcccaat
    39421 tattagaaga cttacaggct tttgaaaaag ctgtccgcga agaagatatc cctgagatct
    39481 accgtttata caatggccga ttgaatgaag aattaaaaag aagctcaaat gaaaatcacg
    39541 agatcgatca attgctgatg cgtaagatcc atgatcgttt tttacaggaa tttcctttta
    39601 tggaacaagt agaaaacatc tcgccaacga tgagctatta caaaatcggc acttattacc
    39661 atgatcggcc cacggtcggt attgatgcaa gtctgccaga aatttttgtt ttgcctaaga
    39721 tcgatgaaga gtgggagaaa tattcccaac cggagtcaac ggactatgac aaaaaaatca
    39781 atgagctaga cgcaaaggtg atcactgctc agacagaaat tgaacgttta gatgaacaaa
    39841 taaaagaaat caatcgacaa atgactgatt tgaacgacaa taaaggattc ttgaatcgta
    39901 aaaaaatcga ggaagaaatt caagaattag agaagaagaa gcaagtacta tctaatgaaa
    39961 aattaggctg gcttccttat attgagacgc ctgaaacaat ccagcagcaa aaagaagcat
    40021 tgaagcaaga agcaagagca gaccagcttc gtgcagccat cgtagaaaaa gaacaaaggc
    40081 aaatcagacg ctatttcggt agcaaagagg gattcggtca agcaatccat gaattcttga
    40141 tgaattatct tggcaatgag aatcctaata atcaatctaa tgaaggaggt tccgagtatg
    40201 aatgaacacg atgaagcaga atggacaaaa aatgaagaac aaacagaaga tcatgaagaa
    40261 acgaacactt cattggaaga acaaacaagt aatcctctgg atgacacaga agaaatccct
    40321 atgctagagg aaacaaaaat acatgaaaaa gaaaaagtaa ctccgacgaa tacgaatact
    40381 gcagaaaaca cccatgctta cgaaataact gaaaatcccg ggtatttaga aggaaaagag
    40441 caagatcatc aagatatcgt cagtttgatt gaaacagcgg aagcagaatt agcggattta
    40501 cgtatgagaa aagatttgat cgaatcagat tttccacaat tattagaatc atatgatcgt
    40561 tttatattga atgaagaacc aatctccacg atcggaaaga caagcctgat cgaatacttc
    40621 acgtatgaat tactgaaacc gttgaatgat attggtctgg aacggtcaaa ttcagcttat
    40681 gacacatgga aaacaagaca tttttcgatc aattatacgt tgaatggaca aaacgaatta
    40741 gtcttttcct ttgtattacc gaccatcgat caacgatacc aagtggaatc gatgagtctc
    40801 ctaaacgtat caccagagac aatggaaatc aatgtccaag atgaccgggt gctttctttg
    40861 atccgacact ggtcagtgga tcgagtattc tctgcagggc agatcacgat attcaatcac
    40921 aaattgaatc aattattagc ccatgcaaga acacttggtt ttaccgttaa tcagacatta
    40981 ctagacaata cgaagccact tcgtttgaat cttcagagcg aatttgaatt gacgaatgaa
    41041 gtattggatg atatttttat cgtggcaatg aaaaatccat cctacgatat ggaaaagatc
    41101 aacgaaacta cttatcaagt acgattagat aaaggacagt ctttaacgat aacaacaaac
    41161 gaaaaagatc aaacagtctt gtcgatctca tctggagact acccacgatc tgtcattgat
    41221 ttctttatca attacgaatt cttggtccca ttaatggtga gaaagtcctg attggataag
    41281 ttggattacc agatgtcatt cagataattc tgttccaaag gacttgcggc agttatccag
    41341 aataattgtc acaagtcttt tctgtatgta ctaaatggac gtgaataact tgctgatgaa
    41401 ggagggattt cattaggaat gtccacaaat aaaatagcac agcttagctt attgtctgct
    41461 gcttgtgtag caggtaggat cgcttttcaa tttattccga atttccagcc aatgactgct
    41521 atttttttat ttatagtact ttacttaagc ttaaaagatg cgttaattgt catgagtctt
    41581 tctattgcga tcagcagttt ttattttggt gttggaccat gggtcatcgg ccaatggatc
    41641 agctatactg ttgttctgag tttgttttca atcgtctgct tgagaaaaac agtgcaaagg
    41701 aatctatggt taaagggagt ctgttttttt ctggcgggca tggtttacgg aataggtatg
    41761 accgtttttg atacgctgtt atatcgtttg cctcaacctt ggatctatta ccttcaaggg
    41821 gtgtcgtttg atttgatgca cagtataggc aatgttgtgt tctttcttgt ttttctgcca
    41881 gtcgtcaaac gtttttacaa tcattcagga ggaaaaaatg aaaaaaataa tttatagttt
    41941 gcttgcaatg agtgtcagta tactcatttt gacaagttgc agtacaacct atacacaatc
    42001 ggcttccaac caaaaggagg aagtaaaaac atcgcaaaaa gcaacaatca cgttacaaga
    42061 aaatggtcaa aacttttcgg aaaaagaagt ggtcttcaaa aaaggcgatg atttactgag
    42121 tattttgaaa cgaaattttg aagtcaaaga agataacggc ttcatcactt cgcttgaagg
    42181 gcactcacag gatgagaaaa atcaactcta ttggatgttt actgtggacg ggaaaagtgc
    42241 tactactggt gccaaagaga tcaaattgaa tgatggacaa tcggtcattt tcaatttgtc
    42301 taaactataa gtttcataaa aaactgttaa agggcgaata ttgccataat tcagtgagtt
    42361 cagttgatga aagaaggaaa ataacatgga aaattttaaa aaaagcggaa tgttttccgt
    42421 aattgctgct ctaggggcta atattctagt tgctatcagc aaatttgtcg gctatgcaat
    42481 cagtggcagt gccgccatgc taaacgaaag catccacagt atcgttgatt gcagcaatca
    42541 gattttgctg ttattcggtg ataaacaagc caataaagga cagagcgagt tgcatcaatt
    42601 tggtgaagga cgagcaaaat actttttcag tacaatcgtc gcaatgatgc ttttcttcgg
    42661 cggtggggca cttggggtga tggaagcttt tgaaaagttg acgcatccat cccatgaagt
    42721 gggtaatccg atcattgtta tagcaatact gttatttggt ttggtaatcg aaggaagttc
    42781 gttacgcgtg gcaatgaaag aaatcaaaga attgaatact gaaaaacttt ccacgatgcg
    42841 ctttttgcgc gaaagtcgac acagtgaaat actgattatt tttacggaag acttgtgtgc
    42901 tgtaatcggt ttggtcttgg ctttagcagg aacaatattg acttatgcaa ccgggattgc
    42961 tgcatttgat gcgatcagcg gattgctgat cggtttcttg ctgatgggag ccgccatttt
    43021 cctagcaaaa gaattttata gtctgatcat cggtgaaagc gtgacagcaa gcgatttatc
    43081 aaaaatcaag ttagcttttg aacgtcctga gatcagtcgg ctgatcgata taaaaacagt
    43141 tcatttaagt ccaacggaga ttttagttgc agcaaaagca gatatcgttg gtaacaaaga
    43201 gaatgaagct tactcggtca tcaatgatat tgaaaaaaat atgcgtcagt ctcttcctga
    43261 caagaagatg tatatataca ttgaaacaga taaatatgac ccaaattatt ctcgtaaata
    43321 aaacgtttat tcatgaattg aagcaaaaat gagaaaaata taatcttgtt tttaaataaa
    43381 acggctacaa tttagcgaat ttatgttaca atgagtccgc tgtttatttg ttgtaattaa
    43441 aggagatatt ttaatgaaca aaaagaaaag tgcgccatgg cttcaagctt tgcatgcagt
    43501 gatgccactt tgtataagct atataccagt aggattagct tgcggagtat tgttgcaaaa
    43561 agttggattt gatcctctct tatcaggact gttatcgatt ctggtctttt ccggtggcgc
    43621 gcagttttta gttgcatcta tgcttacgac gcaagcatca tttgcaacga cattattgat
    43681 ggttttcttt ttagaattac gctatacatt attaggagca agtcttgccg gttttatgaa
    43741 aaaagaaaag cggacgtttt tggctgtctt ttctcaatca ctgaatgatg aaaactatgc
    43801 ggtgaactac ttaaagtttt caactgaccc ttcctggaat aaaagaaagg ctttatatgt
    43861 caattggttc tcaatgactg catgggccgg tagcaatatg atcggtaatt ttttcggctc
    43921 ggtgattcga gtagatgctg accttgtcca ttttgctttg acggccatgt tcattttcat
    43981 gtttatcatg cagatgaaaa gcgcactact gatctttaca ggtttattct caggcgtatt
    44041 aggggtcgta ttcatggtat tattccaaaa tactttcgga ttgattgcag caacagtcat
    44101 tgcttcattt gccggattta tgctggagaa ggcattcaaa aaagaaaaag ctaagaaact
    44161 aagagaccgc ggtaaagatg tcaatgagcc gctcttcaac ttcccggaac aggaggccaa
    44221 tcaccatgac taaactatat ttgctacttt tagttgcttg tctgttcatc gtggcatata
    44281 tcccgcgctt gttcccgatg ctttatttta ctcatcgaaa agtgccatcc tggttcagtg
    44341 actggatgaa atatgtaccg gtggcattat ttgcggcgtt agcttttaaa gatgttttca
    44401 ttactcatga acatctggat attgcttgga atatcaaaat agctgcaatg attctagtag
    44461 caggcgtggc ttacaaaaca cgttcgatgg cgttgtcagt tttgacagga cttgcgtctg
    44521 tctttttgct gtctatgctt taaactgttt tattttatta aaaacagaga ttgcgacata
    44581 agttgttcaa actgatctat ccgaactgca tttgcaacag tcaattctgg atgcaagttt
    44641 attcgtaggg ataaaaaaga ttagtgttgg cttatgatgc aagctctttt ttattttagg
    44701 aattagagag ggatttgtcc ttctatgatt ttttgctata atagggaaga tgtttatttt
    44761 atgaaagaat agttgaggga atttaaatga ataaaaaatt gatgcaagga acattttggc
    44821 taacctttgc taatcttcta tgtaaagttt taggggtagt ctatttgatt ccctggctaa
    44881 gtatgatggg gaacaatcaa gatggtatgc ttgctacgac attgtataat gttggctatc
    44941 ttccttatgg attgttcctg atgcttggta cagtcggatt tccaaatgcg attgcaaaaa
    45001 aagtagctgt agccacaaaa gaaggagata acgctgcttg tcggctgatt tttagaagta
    45061 cgatcaatat catgtttgtc atcggtatcg cctctgctgc actgatgtat ttatttgctc
    45121 ctttactagc cggaatcagt ccgattgcta acgttaataa tgggatacta gctatcagaa
    45181 gtttatgccc atctttgatt gctatcccaa ttcttagtgc aatgagagga tatttccaag
    45241 gaaaaaatga tctgcggcct tatggaactt ctttgattat cgaacaggct gtgcgagtga
    45301 tcgttatctt ggctggtact tattacttgc gcgtattgac tgacggaacc atattagaag
    45361 ctgtattgat cagtacagtc gcttctttct tcggtgggtt agctgcgatt atccatatgt
    45421 atatcgtcgg acgcagaaaa gacttcttcg aacttaaaga ttttttgatt tccagccgtt
    45481 atttcgaaag agaaaaccga tctgcctctg tctctatcat tcgggaaaca ctaccgttta
    45541 tttttgtcgg ctcagtcatt actatcgttc aaatgatcga ccaagtaacg atgaaaccac
    45601 tgcttcactt tttccgtcca gagatcgcag atcagcaact ggaattctta ttcagccgtg
    45661 cttctgtcaa tccaaataaa ttgacattga tcctgatctc catggtagga acagtagcta
    45721 taagcagctt gccaatatta agcacgatga agaaaaaaga tcggatacaa atcgaaaaaa
    45781 cagtaggaga tagtttttcg attgcgttat tgattcttct tccttcattg actggaatgt
    45841 ctttattagc tggaccacta tacacattgt tttttggcta tgatcctgaa agtgtcggat
    45901 atttccaaat ggctttgcta gcttctttgt ttttctcttt attcacgatc ctatctacga
    45961 tgcttcaatc tttgaatcac catcgttttg cgatccgatt aactgtagaa gcaattatcc
    46021 tgaaagtcat cttccaagta atcggattag gattagttgg cggatacggg atgagcttat
    46081 caaatggctt agcattcggt attgtctttg ttcgcggcta tcactttatg tgcaaagaat
    46141 accgaatagc acctttagca aagatcagca attttttctt aaaaactttc cgctccaccc
    46201 tgattatgct tgcagtatgt tttgccgttt tctttttgct gaatcagcga ttgtcaatgg
    46261 aatcaaaaag ctatgcaatg atttattgtg tggctgtagg aagtattggc ggcttagtat
    46321 ttttattttc tcaattcgga aaaaatgggt ttcgaatgtt gaaaaatgtt aaacatcata
    46381 gcagaagcaa agaataggtt aactaatcgt tttttgctgt cacttgaaaa acggcaactc
    46441 gtatgattac gcttgtcgtt tttctatttt ccaattttag tttcatatct aatcttactt
    46501 atgatatagt tataaggtct ggaaaaattc tttgaaacga ggaaattaaa cacatgaaac
    46561 ttgcagtagt tacagatagt tcggcatatc tgccggaacg tatcaagaac catcccgact
    46621 tattcgttat tccaatccct ttgattatgg atgggaaaat ttataacgaa ggaatcgata
    46681 tcgaagcgga tgaatattat ggacttttaa aaaacagtaa agattttcct actacttcac
    46741 aacctgtcgt aggggaagtt ttgggtttat atgaggaatt gaaagagaaa gggtatgaca
    46801 cgattttaag tatccatctt tcttcaggga tttctggctt tgtcaacaca ttatttgcga
    46861 tgaaagatga catcagcgga gtaacggtca ttccttatga ctctaagatc actagtatgc
    46921 cgatgggaca catggtagaa gctgccttag acttaaatga tgaaggtcag tcccttgatg
    46981 aaatacttgc acatatcgat cgaatccgag ataataccta tgcttatctg attgtagatg
    47041 atttgaacaa tttagtacga ggaggacgtt tgacaaatgg tgcagcgctg attggcagct
    47101 tattaaaaat caaaccgatc ctgacatttt ctgaagggaa aattgtttta ttcgaaaaaa
    47161 tccgttcaag taaaaaagca tttgctcgag ctgaagaaat tattggtaga agaaataacg
    47221 aaatacaacc gccagtcaaa ctttacgtga tacatgcaaa taatttagaa gtagcgattg
    47281 cggagaaaga gaaattgcag aaaaaatatc caaatgcaga aatcgaaatt ggtcatttcg
    47341 gaccagtcat tggtacacat ttaggtgaaa aagccatcgg cattgccatt tcagcgcaat
    47401 aattcctttt attcaaaaaa cataccaaaa ata
//
LOCUS       DAZKNH010000010        46056 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000010 DAZKNH010000000
VERSION     DAZKNH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.010"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..909
                     /locus_tag="ACG8MG_000535"
     CDS             28..909
                     /locus_tag="ACG8MG_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0374606.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            922..2532
                     /locus_tag="ACG8MG_000536"
     CDS             922..2532
                     /locus_tag="ACG8MG_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0374607.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2609..3268)
                     /locus_tag="ACG8MG_000537"
     CDS             complement(2609..3268)
                     /locus_tag="ACG8MG_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0374608.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3551..4216)
                     /locus_tag="ACG8MG_000538"
     CDS             complement(3551..4216)
                     /locus_tag="ACG8MG_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFQ0374609.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4363..4968)
                     /gene="dhaL"
                     /locus_tag="ACG8MG_000539"
     CDS             complement(4363..4968)
                     /gene="dhaL"
                     /locus_tag="ACG8MG_000539"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFQ0374610.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4996..6006)
                     /gene="dhaK"
                     /locus_tag="ACG8MG_000540"
     CDS             complement(4996..6006)
                     /gene="dhaK"
                     /locus_tag="ACG8MG_000540"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFQ0374611.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5981..6376)
                     /gene="dhaM"
                     /locus_tag="ACG8MG_000541"
     CDS             complement(5981..6376)
                     /gene="dhaM"
                     /locus_tag="ACG8MG_000541"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFQ0374612.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6530..6850
                     /locus_tag="ACG8MG_000542"
     CDS             6530..6850
                     /locus_tag="ACG8MG_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFQ0374613.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6962..7819)
                     /locus_tag="ACG8MG_000543"
     CDS             complement(6962..7819)
                     /locus_tag="ACG8MG_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0374614.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(7927..9231)
                     /locus_tag="ACG8MG_000544"
     CDS             complement(7927..9231)
                     /locus_tag="ACG8MG_000544"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0374615.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9228..9737)
                     /locus_tag="ACG8MG_000545"
     CDS             complement(9228..9737)
                     /locus_tag="ACG8MG_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374616.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9912..12047)
                     /locus_tag="ACG8MG_000546"
     CDS             complement(9912..12047)
                     /locus_tag="ACG8MG_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFQ0374617.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12130..13785)
                     /locus_tag="ACG8MG_000547"
     CDS             complement(12130..13785)
                     /locus_tag="ACG8MG_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0374618.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13787..14101)
                     /locus_tag="ACG8MG_000548"
     CDS             complement(13787..14101)
                     /locus_tag="ACG8MG_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0374619.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14271..14531
                     /locus_tag="ACG8MG_000549"
     CDS             14271..14531
                     /locus_tag="ACG8MG_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFQ0374620.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14545..14859
                     /locus_tag="ACG8MG_000550"
     CDS             14545..14859
                     /locus_tag="ACG8MG_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFQ0374621.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15589..16143)
                     /locus_tag="ACG8MG_000551"
     CDS             complement(15589..16143)
                     /locus_tag="ACG8MG_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFQ0374622.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16269..18198)
                     /locus_tag="ACG8MG_000552"
                     /pseudo
     CDS             complement(16269..18198)
                     /locus_tag="ACG8MG_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18504..19220)
                     /locus_tag="ACG8MG_000553"
     CDS             complement(18504..19220)
                     /locus_tag="ACG8MG_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0374623.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19221..21044)
                     /gene="glpO"
                     /locus_tag="ACG8MG_000554"
     CDS             complement(19221..21044)
                     /gene="glpO"
                     /locus_tag="ACG8MG_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFQ0374624.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21058..22554)
                     /gene="glpK"
                     /locus_tag="ACG8MG_000555"
     CDS             complement(21058..22554)
                     /gene="glpK"
                     /locus_tag="ACG8MG_000555"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFQ0374625.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22690..24150)
                     /locus_tag="ACG8MG_000556"
     CDS             complement(22690..24150)
                     /locus_tag="ACG8MG_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0374626.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24140..25435)
                     /locus_tag="ACG8MG_000557"
     CDS             complement(24140..25435)
                     /locus_tag="ACG8MG_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0374627.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25767..26444)
                     /locus_tag="ACG8MG_000558"
     CDS             complement(25767..26444)
                     /locus_tag="ACG8MG_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFQ0374628.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26474..27565)
                     /locus_tag="ACG8MG_000559"
     CDS             complement(26474..27565)
                     /locus_tag="ACG8MG_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFQ0374629.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27666..28100)
                     /locus_tag="ACG8MG_000560"
     CDS             complement(27666..28100)
                     /locus_tag="ACG8MG_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0374630.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28684..30141)
                     /locus_tag="ACG8MG_000561"
     CDS             complement(28684..30141)
                     /locus_tag="ACG8MG_000561"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0374631.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30138..32033)
                     /locus_tag="ACG8MG_000562"
     CDS             complement(30138..32033)
                     /locus_tag="ACG8MG_000562"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0374632.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32091..33866)
                     /locus_tag="ACG8MG_000563"
     CDS             complement(32091..33866)
                     /locus_tag="ACG8MG_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0374633.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33916..35412)
                     /locus_tag="ACG8MG_000564"
     CDS             complement(33916..35412)
                     /locus_tag="ACG8MG_000564"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0374634.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35606..37228)
                     /locus_tag="ACG8MG_000565"
     CDS             complement(35606..37228)
                     /locus_tag="ACG8MG_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0374635.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37556..38869)
                     /locus_tag="ACG8MG_000566"
     CDS             complement(37556..38869)
                     /locus_tag="ACG8MG_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0374636.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39114..39338)
                     /locus_tag="ACG8MG_000567"
     CDS             complement(39114..39338)
                     /locus_tag="ACG8MG_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374637.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39358..39780)
                     /locus_tag="ACG8MG_000568"
     CDS             complement(39358..39780)
                     /locus_tag="ACG8MG_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0374638.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(39922..40632)
                     /locus_tag="ACG8MG_000569"
     CDS             complement(39922..40632)
                     /locus_tag="ACG8MG_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFQ0374639.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40650..41147)
                     /locus_tag="ACG8MG_000570"
     CDS             complement(40650..41147)
                     /locus_tag="ACG8MG_000570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0374640.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41184..41636)
                     /locus_tag="ACG8MG_000571"
     CDS             complement(41184..41636)
                     /locus_tag="ACG8MG_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0374641.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41771..42376)
                     /locus_tag="ACG8MG_000572"
     CDS             complement(41771..42376)
                     /locus_tag="ACG8MG_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0374642.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42826..43446)
                     /locus_tag="ACG8MG_000573"
     CDS             complement(42826..43446)
                     /locus_tag="ACG8MG_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0374643.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43448..44317)
                     /locus_tag="ACG8MG_000574"
     CDS             complement(43448..44317)
                     /locus_tag="ACG8MG_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0374644.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44340..44747)
                     /locus_tag="ACG8MG_000575"
     CDS             complement(44340..44747)
                     /locus_tag="ACG8MG_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0374645.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44766..46055)
                     /locus_tag="ACG8MG_000576"
     CDS             complement(44766..46055)
                     /locus_tag="ACG8MG_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0374646.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        12922 a         9837 c         7889 g        15408 t
ORIGIN      
        1 atttttagaa aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa
       61 aagagctttg gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa
      121 gtcgttggat ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta
      181 gggattataa aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat
      241 agtttatcta tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat
      301 ttgactggag gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa
      361 aaaaaagatg aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat
      421 tcaaaaggaa atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc
      481 tatttatttg atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa
      541 gaagttgaaa aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt
      601 gaagtcgagc gactagcgga taaagtcgtg atcatacgcg aaggaacact cgatgaagaa
      661 ggaacactcg atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca
      721 gagaatagtc acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc
      781 gctcaatttg ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta
      841 ggtgtgaaaa aatttgaatc tattccaccg actcttgagg atttattcat gagacattat
      901 gaaggatgag gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct
      961 caaaacctta gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt
     1021 tctggtggtt ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta
     1081 tacgaaacct tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat
     1141 gctgctgagt atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg
     1201 tttgctgctg tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt
     1261 ggtctgattg aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc
     1321 atcgctgaaa gtattctgat caatttgctt ttagcaatat cgatcagctg tacgatgatt
     1381 tttttccaag cggattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt
     1441 agtatgggga ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca
     1501 gccagttcga atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata
     1561 acagatgtag ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc
     1621 tatccattta caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc
     1681 ttgataatca gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc
     1741 gaacgtgaag gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc
     1801 tatctaaata aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca
     1861 tacggctcta tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag
     1921 atgtttatcc aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtagtc
     1981 ttgatttgtc tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa
     2041 acccacggac gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg
     2101 accaatatgt tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca
     2161 ctagggcttt ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt
     2221 cttaaagccg ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt
     2281 ctattcggat ggcttcctcg aatcggtaaa cttgcttata tctacttagc ttactccttt
     2341 gcactcaatt actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc
     2401 cctagctggg ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta
     2461 tcatgtatca gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta
     2521 ctagaaggat aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa
     2581 atccaacatt tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga
     2641 tccagacttc tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc
     2701 tagataaagt ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca
     2761 ggaaaaagtg atccttccct gtcgctttag ctaagtcaag cagatagaca gcgactcgtt
     2821 cttctacttt ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa
     2881 cgttgattgc taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat
     2941 ctttttgtct caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat
     3001 ttggaacgcc tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa
     3061 gctgttcctt tccgctagca gataattgat acactttcat actgccggag gccacgatca
     3121 ctaattgagg ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt
     3181 tgacaagtgc gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa
     3241 atgaaacaca taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa
     3301 taaagaaaca taacttccct tattccaact gataattttc ttttatcata gcataaacgt
     3361 caagcgaacc agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc
     3421 ttcttttgag accttaagta attgattcct atcaattttt taaatattta ctttttgaat
     3481 aatcaaaaac aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat
     3541 ttcgtgttgt ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt
     3601 gtaaggtttc agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt
     3661 tacccatttg aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat
     3721 cggacaaata gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata
     3781 tgtaataatc cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt
     3841 tatacgctgc aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga
     3901 tttggtattg tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt
     3961 cgcttcggta cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga
     4021 ttttaggttt gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct
     4081 ctttccattt ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg
     4141 gatggctctc tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg
     4201 attttccaag ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt
     4261 gcgatatgga caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc
     4321 aaacaaaaaa ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac
     4381 atctaaaagc gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat
     4441 tgcacgctct cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc
     4501 ttttgcctgg tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg
     4561 cagccaaaca tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc
     4621 cgctgttccc tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc
     4681 ttttgtcgct ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc
     4741 gactttcgat atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg
     4801 atcggcttga aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc
     4861 tccatcgcca atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc
     4921 tgcaaattgc gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt
     4981 ttatcttgag attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca
     5041 ttgtgtctcc gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat
     5101 aaaattaccg acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac
     5161 atcattcatg aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac
     5221 acctgcttct tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc
     5281 cagtacttta gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac
     5341 accatactcg atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt
     5401 tgctgctcga agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc
     5461 tttcaactca gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag
     5521 gacagtacct gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc
     5581 atcatctacg atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat
     5641 atcgaagttc aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact
     5701 atcggcttcc ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc
     5761 aagcaccgct gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc
     5821 gcttcctcca ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg
     5881 ggaagattct acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc
     5941 ttcgacaata ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc
     6001 cggcattttg tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg
     6061 ctgctgcaaa agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct
     6121 cttcttccaa catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa
     6181 aaacaaaata actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt
     6241 ctgatccgat ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt
     6301 gcatctgctc aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca
     6361 aaatactttt tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg
     6421 ctttcattaa tgaacaattc aagtatataa taaatttcta attttgggaa gatatgtgta
     6481 gtgcttcttg aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac
     6541 aacaacgaac agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga
     6601 ggtcattact gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg
     6661 cggccgctca aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga
     6721 aatggccgaa cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta
     6781 tgaagtatta ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc
     6841 cacgagataa gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc
     6901 actcaagaat tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt
     6961 tttatttctt tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta
     7021 aggaataact gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt
     7081 tggatggctc tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt
     7141 ctcctgccga atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt
     7201 ttgcttcaaa atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac
     7261 tggccgctgt gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct
     7321 gatttaattg ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga
     7381 cagcttccgc ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc
     7441 ccactttatt tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt
     7501 tgttttttgc agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca
     7561 aatgatcgat ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa
     7621 tcgtttctcc tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt
     7681 tttcagcacc agaagagctt atggttgttg aaggttctgt ttgagcaggt tcagaagttt
     7741 ccgcaaaagc tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg
     7801 gcttcattct ttttttcatc acttttccca tccttactac tttttaacta atttcttatt
     7861 ataagcaaaa atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg
     7921 catcttttat ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat
     7981 cgttttgact gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa
     8041 aagcatatat agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat
     8101 cagcgaagct agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact
     8161 gaatgtaaaa gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa
     8221 tccagcaaaa agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg
     8281 atcaatgaag atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa
     8341 attcgtcagc cagacttctg ttccaccttt tgcccaacgt tttctgtgtc gatatagcat
     8401 cttcaacgtc acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca
     8461 gtcatttaac tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa
     8521 tccgcccaca tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata
     8581 aagaccaccg aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc
     8641 aaccgtttga gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac
     8701 cgcagcaata tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc
     8761 tggctccggt accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag
     8821 tccgatatta aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt
     8881 agcgtatttt ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc
     8941 tgtcactaat acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtata
     9001 ttcgatcaca atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact
     9061 caagggaaca tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac
     9121 accaataaac catacaaatc cgcctaaaat cggataagaa acgagaaaaa aatacataat
     9181 agacaaacaa gtatctaaga ataaattacc tgtatgggtg attgagatca tttcagttcc
     9241 accccatttt ctttatacaa atctgcaatg aatgtcgtat ctagattttt ctcttcggag
     9301 acagaataaa aacggaccgt ttctctctct tcttttgttc caaaacgttc cgtatacact
     9361 tcttcaagta attcttgtct ttttttcaat ttttcatctt catattcaaa agatttattt
     9421 acttttttgt atagatttcg attgttccac ctagtcaata caaatgaggt aatcaggaaa
     9481 ctgaaaaaaa tcaatacaaa aaagccgatt aagaaataca gcattttctt ttctgataaa
     9541 aaagcgtaca aggaaaaatc cttggctctt tctggtctcg ttaatgggaa aaagacccat
     9601 ataaaaggaa gaacaacgat catccatcct aaaagagcca ctaacgtttg ccaaatcttt
     9661 aaccagtaat taccttttga aaaatactta tctttcacca aaattgatgc aacttcttct
     9721 cttgttactt ttttcatatc ctcacctcac gtttttttgt tacgacgtag gagtttacca
     9781 tacatttcat ttcttttcac ataaagtgtt cctaaatttt tcctaagaaa aagaacaaaa
     9841 catttctcat cagcaaatga aaaatatttt gttcttttta tcagttgttc tttttatttt
     9901 ttatctctta attatgttgc tctttccaat acgacaatgc ttttcattcc tctagatatc
     9961 gccgtgtaca tttgtctgcg tcttctttgc tcatctccat cgttttcaaa tggatataag
    10021 attacatgat caaattccag tccttttgct agactcaaag gaatgatttg gcagttttct
    10081 aatccatatg aatttttttt aatcgattct tgtagcgttt gggtttcctc ttctgtttgg
    10141 gtgataactg ccgctttttc tccttttaga gaaaccaaaa tctcagctag tattttcaac
    10201 aattcttcag aggatcgaaa agagcggtat tcgggttctt ttccttgcgg tcggatcgat
    10261 acgatgtcga tcttctcttg atccacagca taggttttga aaagctctgt gattgctccg
    10321 ctagaacggt aactatttct aagatcatac cgatggatcg gcagatggta gtcgtcgaaa
    10381 caacgcataa tatcagcaaa agtagtggaa gaattaaaga ttgcctgatt ctcgtcacct
    10441 actaaagtaa agtgagtttt tggaaataat tcgatcaata gacctaattg tgccagggta
    10501 taatcttgta cttcatcaat caatagatag cgaaatgctg gtacatttac cttttcaaca
    10561 agtaaatgac gaatagctaa tataatgact gcttcatcaa cagtacgtgt gggttgccaa
    10621 gcataagctg agccatatcg tttttcataa attctttcaa acaactgatg ctcatccacc
    10681 catgccattt cttcgatttg tctggttatt tttcgatatt ttttttgtaa aagtttcctt
    10741 gcataagcta caatactttg ttccgaatca tcagaaatca atttaccgaa taattccaac
    10801 tgcctatctt ctgtcaacga gagtacctga ttctgcacag caggactttt tgcttgtttc
    10861 aaaagatgat tcttccattc gctcatcagt gcctttttcg ttgcttgaag cctatcgatc
    10921 aatttaggat gagcaggaac tttctcatat attttttcga tcgttttttt agaaaggact
    10981 gtttttccat ttttagtaag tccacggata aaattcggat gatctaaaaa caaaggatct
    11041 gattgtttta gattatcgat aaactttttg ctacgcaaac gttctgtctg ctccgataca
    11101 ttttctcccg tgatacgttt gaaataagcc tcttcacctt ctatttcctc tgctgaaagc
    11161 tgtgaaacca gctgtatgat ggttagattc agtggatttc tttcacctaa tgatggtagg
    11221 acattcgaaa tatagtcgat aaaccgatga ttaggtgata gaatcagcaa gtcatcggat
    11281 gtcatcgtat ctcgatataa atagagcaga tacgcaatac gctgcatgat agcagaggtt
    11341 ttaccacttc ctgctacgcc atttaccaag atattttgac ttttcacatc ccgaatgatt
    11401 gtgttttgtt ccgattggat cgtagcagtg atagccttca tttcattggt ctcatcttgg
    11461 cctaagactt ctaataatac gtcatcttga atagctacag acgtatcaaa aaacttgacc
    11521 aaacgatctc tttccaaaag aaattgtcgt ctttctttaa taaccgtatc gattttattt
    11581 tgacgaaccg tataagaaga tggaccaatc atgttattgt aaaaaagttc ggcaattggt
    11641 gaacgccaat cataaacaag attttcatct gcatcatctg tgaaatttgc tgtaccgata
    11701 taaaaggatt cttttggctc attatccaga aagtctacaa cgacttttcc aaagtaggga
    11761 cttttcaata aacgttcgat ctttgctagc tgattttctg cgtttttctg tttgatattc
    11821 atctgatcga tttctctatt tttcatttca atcatcgaaa aagtatccag gttatctaag
    11881 taactgtcaa aattcaatcg aagctcgcca gccatttctt tcaacgactg gtttccttct
    11941 atttttgccg tatccatcga ttcccgcagt tcgttttttt tatcaattag cttttgatac
    12001 acgttcttca gatatgcttc ttctaactgt ttttgtgtgt tgaccataca cattctcctc
    12061 tttttttaaa gtggtggaac cgattagttt acctctttta agagtcgaat gcaagctttt
    12121 taacgagtat catttgttag gatatatcag ttcttcaggt cggacagttt gatataaaga
    12181 aaatgcacca tcaaggtttt ttacgtggta gccagactgc ttcaagattc tttccgctag
    12241 atagctacgt aatccgctat gacagctgac gatatattct tgttggctgt ctaattcatt
    12301 taatcggtcc cgaagttcat tcaaagggat atgtttggct tccttgaatt tgcccttttc
    12361 caattcttct gctgttcgca catctaataa aatttttcct tttgctagtt ctgtcggcaa
    12421 ttcatgccat tgtacggttt cactaagccc ttctgcaatg ttcattgctg catatcctag
    12481 catgtttact ggatctttgg ctgagccaaa tggcggtgca tacgtcaatt ctaattccgg
    12541 taaatcgaag atcgtcaaat gccctttgat tgctgttgct aaaatatcga tccgtttatc
    12601 cacacctttt gccccaacac cttgtgcacc ataaatctcg ccagtctctg gatggaagat
    12661 caacttcagt aaaatatctg ttgcttctgg ataatagctt gcatgatctt ttcctgatac
    12721 atgaatgact tcaaaagaca gttcgttttc tttggctgtt ctttcactta aaccggtgga
    12781 tgcggcagtc attccaaacg ttcgtataat tgctgttcca ataccgcctt gatttcttcg
    12841 agaaattccg gaaatcgtgt ccgcaacttg tctaccttga cgattggctg gtgaagcaag
    12901 agaaataagt gcatcttttc cagtgatttg ctgttttaca acgatagcat cccccaccgc
    12961 ataaatatca ggaatattcg tttgataacg ttcgtctact aggatacccc cgcgtatgcc
    13021 taattcgatt ccagcggtca ctgctagctg gttatcagga cgaacaccaa tggataaaat
    13081 aactaaatca gcagggatgg tacgcccatc atccagtcgc aatcgtttcc cgtggtcttc
    13141 aaatccagcg acagcatgag aagtaatcac ttctactcct tgatgagaca attctttttc
    13201 tataaaagct gccatttctt catccagtgt tggaagaata tgtggtgctt gttcgatcac
    13261 catgacttct aaacctcttc tttttaggtt ttctgccatt tccaatccga tgaatcctgc
    13321 accaacgatc acggcacgct ttggctgttt ttctaatgca tgcatcactt tatcgatatc
    13381 tggtacattt ctaatagaaa aaacattgtc ggcttctgtt atccctggaa tcgatggaac
    13441 aactggtttt gcaccaggag ataaaaccaa tgcatcgtac tgttcaatgt gtttttgacc
    13501 ctcatgtacg acttctaccg ttttgttttc gggaaagatg gctgtcactt catgatttgg
    13561 acgcacatct aaacaaaaac gagcggataa tgattctggc gtttggacaa gaagattttc
    13621 ccgttcactg atttctcccg aaagataata aggcaagccg caatttgcaa aagaaacata
    13681 cggtcctttt tcaaaaacaa cgatttctgc atcttccatt aatcggcgaa gccgtgtagc
    13741 agcagacatt ccacctgcta cacctccgac aataacgatt ttcatcttat ttccctccta
    13801 ctgttcgatc aaaccattga ttcatgccac cacgtacatt cactacatca taaccagatt
    13861 tctttaattc tttagctgct tgtttactcc tcatccctga ttgacaaatc acatagacgg
    13921 tcttttcttt gtctccatca tatctgttta tactttgtaa tggaacattt ttagctcctt
    13981 ttatatgccc gccccggtat tctgcaggtg ttctaacatc aagtaacgct ggattttcca
    14041 aaagcttttc ctttaattct gggactgtta tcgattttat tgattgaaat agcaaattca
    14101 ttttctgcac ctcatttaca tttttataaa tacctcatgg ggtatattaa acaggagctt
    14161 tattaatatg tcaaccagaa tgactcttca tttttttcac tactttttat atttgcataa
    14221 aataccgata aggggtatta tctaattata aaaatagagg agaatgtgtt atggagattg
    14281 ataataaaaa tatcctgaac cgtttgaaac gtacagaggg acaaattcgt ggtatccaaa
    14341 aaatgatcga agacgaaaaa gaatgtatgg atgtcatcac tcaattaagc gctgttcgtt
    14401 ctagtattga tcgcgtaatg ggcatgatcg ttgcagataa tctgaaaaat tgctttgaga
    14461 aacctgatag taatccagaa gaacaagcgg ctaaactaga acaagcgatc aagatgatta
    14521 ttaaaaagta aaggaatgat ttcaatgaaa gtcgttttcc atattgatga attagaaaaa
    14581 tggtcagaaa cagggaaaaa cgtaaaaaac ctgataaaag cttctccaga aacgacgatt
    14641 gtcgtatctg taaatggcat cgcgattacc ggttatctag actctgccaa tgcagaattc
    14701 ttagatttgc aaggagtcgt ttttcacgct tgcgccaatg ctatgcgtgc taatcatatt
    14761 tcagaaagtt ctttacctga acaagtaatc gttgtaccag caggtgtcct tgatttagtt
    14821 gaactgcaat cccaaggtta tgcttatatc aagccttagt cagtagaggt tgcgacagaa
    14881 gtgatcagct tcaagaaatt tcggcttatt tttcgaagaa gctacttctg gaaaaccgtg
    14941 aatcaaaggt tgtgaaaacg ctgtcctgca tcaagtaata agaacagcca aacaaaaata
    15001 gcttctcata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    15061 tttaaggaag aggttgtgaa atcagcggtt tgacctaagt attagagaaa aaaacgggaa
    15121 aatagctcct catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac
    15181 cgtttaagga agagattgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg
    15241 aaaatggatt tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa
    15301 taccgtttaa ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agaaacaaac
    15361 ggaaaaatag ctgctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg
    15421 aacaccgttt accaagaggt tgtgagccta ccatttaact ccgaatcaca agaaataatt
    15481 tcaaaaacaa cttctcctgt ttttggttcg gttggcttat tatcgagaag tagctactgg
    15541 gagtctattt atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt attttctttg
    15601 aaacatcttt cggtactcga cagcatacaa gacaaaagat tgtaaaagac cgtaagtgat
    15661 gacggcaaaa atgactctca cccacagcgt tggtaattct gggaaaagca gcaaaaggat
    15721 catgccaaca tagatgacag cagtcgctac tttgccatac cacttcgctc cacttaattt
    15781 gatattttta gatagcccga ttaatccagc tacaaacata aagccttctt ttatcaagaa
    15841 tagaccaaac aacagccaaa gccacggtcg ataccatgcc atagacaaaa tcagaaaaag
    15901 ttgtgtcaat ttatctgcaa acggatcaag caccttccct aagtctgtta cctgattata
    15961 tctccgagca atgaccccgt ctagataatc agacaaacct gagacaacaa tgaccgctcc
    16021 tgctatataa aatgactgtc tcagcacaaa ataaagataa atcggcagca aaaaaatacg
    16081 gaatatcgat aaaagattcg gaatcgtttg tatttcttta cgccagtctg tgcttaattt
    16141 cattttatcg ccctacctcc tcagcactct tttcttttta ttttagctta cacggggaat
    16201 aaaaccaatg atttcactgt ttttattttt ctttacagac actgatatta tgagctaatt
    16261 tgagataatc acttgctgtt ttcatcgttt actcttcttt cttcctgtcg tttgcgcatt
    16321 tcttttacta gtactttatg agcttttttt gatcctcgct ctaggtcacg ctgccgttta
    16381 cgtgttttat aatcgataaa cagactgtct aaatcgtaac ctgtcgggta cagatcttct
    16441 gctgaacgca ccagacgaac acgctgacga ggcacgtgga ttattttttt gtctaaatat
    16501 acatcaatcg tatcttcacc tatatcttca taaactaacg cttctttttg gtattcattg
    16561 acaaatatcc gatcaccttt tgcaaagagc tgacgttctt ttttattctc cttcctattt
    16621 gtaactgctg tcttaggaag aaattctttt ttctgtgtcc gataggcttt ttccgccaag
    16681 taacgctctg cttgatggat caatttcatc gacatatcca ttttatgagc aatccacaaa
    16741 gcttggcttt ctcctgtttc ttccaacccg caactgatat ttaggacgaa gtgcttcccg
    16801 atcgaaagcc atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc
    16861 tccataatgg gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc
    16921 gatcgctagt gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac
    16981 caacgtattc ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa
    17041 tgtactcaac gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa
    17101 gacagcaagc tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt
    17161 caataaaccg actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat
    17221 caagcctcga tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa
    17281 tggatgtctg ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc
    17341 attgatttct cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc
    17401 gatcaataaa tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac
    17461 ttgatattct tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga
    17521 aatcacagtt ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc
    17581 atctatttta tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac
    17641 aatcattgct tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt
    17701 tgactggatt tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga
    17761 cgcgtcgtca ctgactttat tattttttat tttctggtca atcaactctt ctacatttat
    17821 caaatcagga agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc
    17881 aaaaaacttt gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt
    17941 tggctgcaga atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc
    18001 cataaatgga acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg
    18061 acgatccaag acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct
    18121 atagtttcca attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact
    18181 aatcatttcg ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat
    18241 aagggcctag agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt
    18301 attagaatac aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa
    18361 ctttgtttat tggctattcg acttactgat tagataccag ctacactcac aaaacatacc
    18421 ccattcttct aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc
    18481 aaactttagt atgttttata gatttacacc attaatgaat agacacctac cgcgataatt
    18541 gctccgatca ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta
    18601 ttagctattg gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt
    18661 gcatatcctg ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg
    18721 aatacgttcg ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat
    18781 acaaatgtac caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct
    18841 gttgcaaacg ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa
    18901 gaaagccaaa caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga
    18961 acgacaagat tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc
    19021 aaatgagctg ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa
    19081 ccaaatgcga tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc
    19141 gcacacacac catctccaag caaaactagg atcagtgtcc caataaactc ccccatgatt
    19201 tgagtcatat cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca
    19261 atcaatgctt ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat
    19321 ccagcaattg catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac
    19381 agatgatttg tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa
    19441 ccataacgta accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt
    19501 gctaattcaa agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct
    19561 tcttcctcta aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat
    19621 ttagttggat cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc
    19681 tctattccgt attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc
    19741 ttacggtaat cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct
    19801 aaagaactac ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg
    19861 ttcaacacgt cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta
    19921 tcgacaactt tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca
    19981 gaaccagaat taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc
    20041 aatgtgatat ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct
    20101 tcttgtgtga ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag
    20161 taagtcttgt tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg
    20221 aagtatgtgg gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta
    20281 gttggacgca ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc
    20341 caaggaccac tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc
    20401 aggtcacgag ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc
    20461 attttactta ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa
    20521 cgggcatcat tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc
    20581 aaaaacggtt ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt
    20641 cctgtcacat ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg
    20701 aaggttgttg ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg
    20761 tgtggggcaa tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca
    20821 tcaaatgtct tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc
    20881 ccttctgcaa aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg
    20941 actgccacac cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc
    21001 tgcatttttt gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta
    21061 ttcaaatgct tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg
    21121 ctcttcctcc atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc
    21181 tgtatctttc caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt
    21241 ttccaagttt tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag
    21301 atattcattg tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc
    21361 atcttgcatc gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt
    21421 aatgatatct tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg
    21481 atcccaataa ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct
    21541 tgtagagcgt ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc
    21601 tctcagccat tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac
    21661 ctttccattt atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt
    21721 ttcaccggta ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg
    21781 ttcgaatgcc atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg
    21841 tgcttcgctt ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt
    21901 gacttttggt aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc
    21961 tagctgatgg atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc
    22021 ttgtccatcg gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc
    22081 tttttcagct cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc
    22141 tgagaaatag gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc
    22201 attttttaag ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata
    22261 gattggacgt cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc
    22321 aatcccagca atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga
    22381 ctgtacagaa ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata
    22441 ttgcgtgaac tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc
    22501 tcgtgagcta gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat
    22561 tccctccatt atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa
    22621 aagaaactgt caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct
    22681 attttttaat caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat
    22741 gtcgtaaggt gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca
    22801 aaatgtgatc accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc
    22861 gattcccctc agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact
    22921 ttctaaatcg attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac
    22981 attggcatac tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa
    23041 ttgctgcacc gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat
    23101 tttctgctgc tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa
    23161 aaaccattca ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt
    23221 caaacgacga ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc
    23281 ggttggtaac tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat
    23341 cgaaaaagaa acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc
    23401 tgttcgacta agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg
    23461 atagagtaat ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt
    23521 tttcgtaatc ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc
    23581 taatacacgg tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag
    23641 cggtcgctga tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag
    23701 ctcttctccg tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt
    23761 caattctttg atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg
    23821 aacgatggat acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc
    23881 ctgcaataaa aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc
    23941 ctgatactca tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat
    24001 atcttccaaa tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt
    24061 gatagaggct gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc
    24121 ttttctttct aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca
    24181 tatctggcac acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg
    24241 tccatccttc ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc
    24301 tttggagctg tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt
    24361 tgctttgatt tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat
    24421 aaaaaatgct ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat
    24481 tgccgactgg acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga
    24541 catttcgtgc tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg
    24601 gacgaaaagt tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca
    24661 agtagtcatc tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct
    24721 gattgtcggg acgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt
    24781 ccgtctctac aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa
    24841 gtgaaagccc ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat
    24901 aacgaaaaag aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca
    24961 acccttcagc tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat
    25021 tcatgatctt ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg
    25081 ttgattctgc agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat
    25141 caaaatccaa tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg
    25201 tcttttctgt gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag
    25261 aaagttgttt ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc
    25321 atcctaactt cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa
    25381 gataaatagc cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca
    25441 ttcctctttc ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat
    25501 aactcgcttt caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac
    25561 cgtgttctag gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc
    25621 tgaagagatg tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc
    25681 tctgtccaaa taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat
    25741 agttagaata gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct
    25801 aattgatgag cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact
    25861 tctgaaaaag ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct
    25921 aagaatggta cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc
    25981 actgtacgtc cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca
    26041 acaccgtgac aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc
    26101 ccaagatcta ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc
    26161 gctgcataat catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt
    26221 ctgaaatctt catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc
    26281 ggttctaaag gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt
    26341 actaatacat cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc
    26401 ttttcgtact tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc
    26461 tataaaaatt gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat
    26521 aaagctcttt atatgctttt tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca
    26581 ataatttctc tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg
    26641 catatttcaa tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag
    26701 ggatctttcc ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat
    26761 cagaacgagc tattcgatca tacatattta atgaaacatg cagataatct agttttgact
    26821 cacgtaatcg ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt
    26881 cttctggtga gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg
    26941 agaaaaccgt tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc
    27001 attgatcctc tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg
    27061 ctccatgcaa ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa
    27121 atgcttggat tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag
    27181 gacgttttgc tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac
    27241 ttgtcattcg tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg
    27301 ctttcgctag ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg
    27361 gccagccttt tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt
    27421 tagaacgttg tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga
    27481 gttgtgtcgt cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag
    27541 taattgattg atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta
    27601 tcgagaatca tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa
    27661 aaattttatt ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta
    27721 cttgcttcat ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg
    27781 acttccttag caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg
    27841 ctaactgttt tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat
    27901 ggggcggaat agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat
    27961 atcaataaaa taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct
    28021 aatttattca acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct
    28081 ttttcaattg gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt
    28141 tattttttgt caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag
    28201 aagtagccat cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt
    28261 gaatcagagg ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat
    28321 ggctttccac atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg
    28381 tttatatcag aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa
    28441 aatagttcct catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac
    28501 cgtttattga gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa
    28561 aaacagcttc tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat
    28621 actgtttatt cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt
    28681 ttattatagg atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt
    28741 ctttaaccgt ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata
    28801 acgttttgac atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata
    28861 accaaataaa tctactccat ctttgatagc ttccttcatc tgctcaatat gctgcttcaa
    28921 atagtcaatg cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc
    28981 gagtccgttt tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc
    29041 aattctcaaa cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt
    29101 tttaattcct ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact
    29161 gactgcacta gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat
    29221 ttcttcatca cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt
    29281 atacgctgga taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg
    29341 ctcaattgtt ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct
    29401 gatcatacaa ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc
    29461 acttgctata aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt
    29521 ttcttcaatt actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa
    29581 agtcatccaa tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac
    29641 gtaaaactga ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg
    29701 catttcataa tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa
    29761 cacttgatca taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa
    29821 aattcttgtc catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg
    29881 gatgtcttct ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt
    29941 gctgttggga tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt
    30001 ccatttttct ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt
    30061 ttcatctaca tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa
    30121 tccttctgga aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta
    30181 tttttcttat gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt
    30241 gtggtcggat catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg
    30301 cctttacgta ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc
    30361 actgtatcaa taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt
    30421 gcatgtttcg ttggaaaaac tgttgaaact tccccgttta cagaactgta gatttttccg
    30481 tccgttggaa tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt
    30541 acttcagata aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag
    30601 gattcttctt cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc
    30661 atcgctaaga aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa
    30721 gacaaaatac ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca
    30781 ccaaatatag ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca
    30841 ccataaacag caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa
    30901 ctttttactt tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca
    30961 gctacggcag tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg
    31021 gtcattactg gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg
    31081 ccgataacag caccagataa aacagggcta aagttataaa cagctaaata tccagctgca
    31141 atcagctgac ttgcataatc tgcaattgga cctacaacta ataaaataat tgggaccatc
    31201 acgatgatac aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga
    31261 atcttaggct caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt
    31321 gtataccttg ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat
    31381 gcactgacaa tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct
    31441 gtattgaact tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca
    31501 tcagctgctg cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc
    31561 gtacataagg cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg
    31621 gcaaaaacag cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct
    31681 ctctttccac tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact
    31741 tcatttactg catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc
    31801 acaccttcta gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag
    31861 cgtaaccttg ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg
    31921 acaatatctt tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc
    31981 gtagcaatcc ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta
    32041 gtaccccttc taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc
    32101 aaatcactca ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca
    32161 aaaaaaacac gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct
    32221 gtgatttttg tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac
    32281 ggaagcataa ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta
    32341 tggattttta tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt
    32401 tcttcaacta tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca
    32461 tctagaacac taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta
    32521 gtcaaaattg catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt
    32581 tctatccggc attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta
    32641 ttcaaaatac taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga
    32701 ttcaacgctt cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat
    32761 tgaaaatgta ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg
    32821 atcttttgta caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt
    32881 ttgatatggg ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa
    32941 aaacgaataa tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca
    33001 tcattaatca tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga
    33061 ttggaaaaag aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga
    33121 ataaataatg tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa
    33181 acgagatacc gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata
    33241 agaacctctt ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt
    33301 tgttgaggaa accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt
    33361 ctttttgaat tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct
    33421 aatttaggat tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt
    33481 tttgaaagaa agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat
    33541 aagtacgtca caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc
    33601 acctcttgta tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa
    33661 cgtttatttg actggataat gattttcttc tcgtagatag caagcatatt tttgatttca
    33721 gtttggatcg ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa
    33781 tgattcttat ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc
    33841 ttacacctcc tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt
    33901 gcagaggttt aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg
    33961 gataatttgt ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc
    34021 atcattttgt ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc
    34081 cgatacgata tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat
    34141 tgcctcagca atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat
    34201 tcgattcaat tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg
    34261 taattcataa cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg
    34321 ccaactccaa ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc
    34381 gccagtatct acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa
    34441 tgcaatataa tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg
    34501 tgcacagtct tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat
    34561 gacatcggag aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg
    34621 atcacagtca tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc
    34681 atttggatcg attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg
    34741 acaggcttga taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc
    34801 agggaatgta accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt
    34861 aaaggtcatc caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa
    34921 ttcaaaaaag tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa
    34981 gggggtttca taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt
    35041 gattagtctg tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg
    35101 aaatattcgt gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa
    35161 acgaatatcc tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag
    35221 tgattcgtct aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg
    35281 ctggttcatg atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca
    35341 tgcaccttca cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat
    35401 ttcctgatcc ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg
    35461 attcatcaaa acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct
    35521 actcttcatt tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca
    35581 ctcgttaacc atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc
    35641 gtaaatcaac tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta
    35701 aagcttcacg tggaatagtg aacgtctgct tttcactgga catattacaa ataacgagcc
    35761 attgctggtc ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta
    35821 attcatagtc tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt
    35881 acatataaaa aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat
    35941 ttggattgac tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc
    36001 attgcatagg cgtcctagca ttgtctcttc ctttcgcctt gattgattcc aagatttcct
    36061 cttttgtaaa tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg
    36121 cttccgcgat atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat
    36181 aaggggtgcc tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat
    36241 acacatcgtc atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata
    36301 aactattcca tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt
    36361 cagagatact caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta
    36421 tgtgttcgaa ttggaagacc atggataatt catttctttt aggattggaa taaagtttag
    36481 cgatttcggt agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag
    36541 ttgcttgatt catttcctga agatactcat gcagtttagg tccattactt gtgatttctt
    36601 tatccggttc tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat
    36661 cgatccagaa attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca
    36721 aatcaggttg acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact
    36781 gccaagcaga accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat
    36841 cacgccaaat ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc
    36901 acggatgttc atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt
    36961 tctttgcttc ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg
    37021 cttgataatt actgatatca tacccattgt catcattcgg agactcgtat actggagaaa
    37081 gccaaattgc atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca
    37141 aatctccaat accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa
    37201 ctgaatggtg ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt
    37261 tttgcttaat catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta
    37321 ttgcaccttg ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac
    37381 tgttttttga aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa
    37441 acggtgttca ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat ataagaaact
    37501 attttttcgt ttttttctct aatactcagg ccagaacgct gatttcacaa cctctttatt
    37561 tcacattcaa tatccacatg aatcccacaa atacaaagaa cagaccatac atgattggag
    37621 atacttcttt tcctctttta gctgctaaca tggtaattgg atagaagata aaacctaatg
    37681 caatcccatc tgaaatacta taagtcaaag gcatgccgat caagatcaag aatgaaggga
    37741 tcgcaatctc taagtttccc cattcgattt gtcgcaaaga ttgtgccatc aatactccta
    37801 ccataatcaa tgctggtgca gttacttgtg aagtaacaac tgatagtaat ggagaaaaga
    37861 acaagcctaa caagaagaaa atccctgtag taatagctgt gatacctgaa cgtcctccaa
    37921 cggcgatacc agctgatgat tctacaaatg caccaacagg agaagtacca aataaggaac
    37981 ccgcaagcat agctgttgaa tcagaagcta aagctttccc tacacgaggc atcttgttgt
    38041 ctttcatgaa acctgcttgg tttgctaatc caactaaagt tcctgctgta tcaaagaaag
    38101 tcacaagcaa gaatgtcagc actacgaccc acatttgaag agaattgatg tcccctacat
    38161 gttttaatgc aactaaaaat gttggtttca aacttggtgc tgcggcaaca atttcagaag
    38221 gcattggaat caaacctgtg accagaccta aaatcgttgt agctgccatc ccgatgaaga
    38281 tgccacctgg tacacgccga acaagcaaga tagcagttac gactaatcca aaaatcgtca
    38341 accaagtaga accaacgttg agtgatccta atcctactaa tgtagattca ttcgaaacga
    38401 tgatcccacc ttcgctcaga ccaaggaaag cgataaatag accgataccg ccagaaatcg
    38461 catatttcaa atctgctggg atcgcatcaa tgatcatttc ccgcagcttg aagattgtga
    38521 tcaaaataaa aatcaaagaa gcaacgaata cgccggctaa tgctgtttcc catggaatcc
    38581 ccattcctaa acaaacagaa taagcaaaaa aagcattgat cccaagagca ggtgccgttc
    38641 caattggata acgggctaat acccccatta aaacacagcc gatcgcactg gctaatgcag
    38701 tcgcagtaaa cactgctcct tcatccattc cagaagcacc taatacagtt ggatttacaa
    38761 ataagatata agccattgag ataaatgttg taaatcctgc cagtacctca cgttttaccg
    38821 tggtgttcaa tttttctatt tcgaagtagg aaagtatttt ttccttcatc ttccatcccc
    38881 ctaaatccga acattataaa taaagtccgt taaaatttcg atttattaat aaaacaacgt
    38941 gttttatcat atactaaaac aaaacataag taaacaaaaa attcggattt taagcaaaat
    39001 ccgaacgttc catctgatta aatcccttta tctcaaagtt catgaacatt cactttggat
    39061 tacaagaatt tctttattgt gttaataaag tttcttcatt ctagtctcaa aaatcaatag
    39121 tgtgatttga ttttaccatt tcgtttctgt tcttctttga tgaattcttt tgtatcgaca
    39181 ggattcagat tatttagtcc gccaaaacct gtatcgacca tttttcccca gccttttgtc
    39241 ttcttttttc ctgatttgag attctttgct cgaagcttct cactatgttt tgctcgccga
    39301 tacttcaaat aaccttctaa tcttcccatt agatccactg tttttcctcc tttttattca
    39361 atcagacgac tcagcttttc ctataacaaa ataattgcct tcagggtcag aaaaattgaa
    39421 agtcaagatg ccattgttat ctgtgatttc tcctggttct tccatgttct cgtagatagt
    39481 ttcaaattca tccgtaaaaa atactaaaga aggcggtggt cctaatacct ctggtgaaaa
    39541 cttctgcaca aatacttttg ggaatacgcc taattctaat tcttttgaga tgctgagtac
    39601 aatactctta aattcttctg gcaattcgat tgtttcagaa atttctgcac caattttttc
    39661 tacaaagaat cggcaaatca tctctacgtc attgacataa agcatcacac gtgttttttg
    39721 tatcatcatt tgacccttct ttctatttga ttttctttcc tgtctataaa ctgattccat
    39781 tatatcacga aagcgcaaac accttgtgtt tgatactatt caagaaaaat aaagcaatag
    39841 tttctaggat tgctttcctc cacaaacttt ttttacaaaa aaaaaacggc aatgaatgcc
    39901 gctcttctta tttcgattct tttataattc tgcttctgat tcatctttgg ttagtgtgtg
    39961 tctaacacta gttacgccaa atgtttgatc gattttttcc ttcaacgctt cgattgcctg
    40021 tgcgccacgt gccgcttgtt cagcattgat ggattcaatg accagcaccg ctgcttgtgt
    40081 ttcttccgtc agcatcgttc tttgggcttc acgccagttg agacagcgac agatcgctcc
    40141 ttgttcatca tagtagatga tttcttccgg aagtgccgga gcatcggaat ctgcacctaa
    40201 aggcaggaat gattcgccac cttgggcttt tcctaaatgc agatttcctg cgatcttttc
    40261 gatatcttct ccgccgcaag gtacgccaaa agacaaggaa atactattat agatatctac
    40321 aagcggattg attgggaaaa attcccgttc ttgatttgca cgtttcagta aagcttcaat
    40381 agaagaacgt gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc actgagcaat
    40441 gacttcgttt tggctgaatg tttcttctgt aaggtaattt cttgcttctt ttgctgcctc
    40501 attcaataat tgatgatacg tttcttcact ttcttttgtt acgtggttat cgataccatt
    40561 tacggataag atttcgatgg ttgcttcagg aaatacttcc cagaaattgg tatccactat
    40621 tacttttttc atctgttcac acctcttgat tagaataatt gcttgatgaa tagatagtct
    40681 gtttggggat catctcccat ataaaaaaca tgttcgctgt atttgataaa tcctttttta
    40741 cgataaaaag cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat ggctgttttt
    40801 tgcttttctt ttgctttttc aatagctgta tctagtaaaa atgtaccgat accttgatgt
    40861 ttaaattttt ttcgtacata gattcgctcg atttctaaaa aatgatcagc catttcctca
    40921 gattgactcg ttcctgtatt tagtttcagg taacccgcag gctcttgatc gatactcact
    40981 aggaaaaaat cggtttcttt atcttttagc tcttctgtaa gtttttctag tgcataagct
    41041 tgtgtcaagt aatcagctaa atgctccggt gaattatctt ttccaaatgt atctgtaaat
    41101 gtttcgatac taatttgttg tagtaagtct acatctgaag gcgtcatttt tcgaatttcg
    41161 tatttattca cggcttcttc ctttcaatag atccttttat tgcctttttt gacaaaattc
    41221 caatcttcgc taacattctt gcatacttta tttaaaagat attccaattg ttttgcttct
    41281 tcgtcagaaa gaccatttaa tgcgactgca ttggagtagt tgttttctcg aatgatgaaa
    41341 ggatagattt ctgctccttt tttagtcgga tagagtttct tgattttttt attttcttta
    41401 tcttccaaac gttcgatcat cccattggat tctaatttct tgattgctcg ggcagtcgtt
    41461 gtacgatcta ctttgatcat ttctgctagt ttttcttgga tgattccagg attttcgcaa
    41521 atacgtacta aataaagata ctgtcctctt gttaaatcga cttctttgaa ttcgatattg
    41581 ctaatagaat ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc agccatttca
    41641 tcccaccttt attttgtttg taagactagt tcacctcttt ttgttgcaaa tgcaactatt
    41701 ttcaataaaa aaatcaagtt cttttttaat aactagtaaa gtatattctt ttcttctttt
    41761 acgaaaaagc tcaatcacaa tagatctggt ttttgttttc taaaataagt aactgtttct
    41821 tcgttgttcg cttttctgat agctttggca actcttctat actaaaaaat cccatgttgg
    41881 aagtttcatt attctcaata aacttggcct cttcaccgtg gatcgcacaa gcaaaaacaa
    41941 gtttataata ctgaaaaagc tgtggaatat ctttttgttt attcgtatca aaaactgctc
    42001 gcaactgagt agtttctact gttaatcctg tttcttcata gacttcttta cgcacatttt
    42061 cttcaggaga caatcctatt tcagcaaatc caccaggaag tgaccattct ttcgtccgtt
    42121 tatcttcgat tagtaggacc ttgccatctt ttttgatata ggcacgaaca tctacttttg
    42181 gtgttggatg tccttctcca atcgttgtga gcgcttttat ttcttcgaaa gtttcttttc
    42241 ctatttcact gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc tctttatcaa
    42301 atacatcttt tccataatat agaccagctt gagaaagtgc gagtagtctt ttgtattgag
    42361 ccaacttctt gttcattctc ttcccttctt ttttagcaaa tgtatgacta tgtaatcaag
    42421 tatagaagac tggtttaatt acggcaaata aatataatga aatctgcaat aaataaaaga
    42481 atgttaaaaa actcgagaac aaaagacatt cgacttctct ggggctgtga ataaatggcg
    42541 ttcaaaagct tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga cgagctattc
    42601 ttgtctggct gttcttatta attgaggcag gacagctttt tcacaacctc cccactaaac
    42661 ggtgttctcc taccaactcc tcggtcacaa gtcacaatat tcatcaaaca tgagaagctg
    42721 tttttttgaa attgctcctt gtgactcgga gctgaacgac aggctcacga cctctcggta
    42781 aacagcgttc aaaagccaat ttttcgatat taagccattt ctttgttact tattgtcatt
    42841 gatttctttt tctaatttat ccaatagtgg agtgtaatcc acttgagtat agtctgttac
    42901 atcaaattca tatagtgttc ctaactggaa tttgtcatat tttcggtcgg cgatgatctt
    42961 tttgaatgct tctttcgtaa tatgattggt ccctttcgtt cgatcttcca atacgtcacc
    43021 atagtgataa tgatccatga tatagctcaa atgacgatct gattcacgat cacgtttgta
    43081 ccgacgttgc cataacactt caaagtctgc tgttaggcga atcgtgatca cctgatatcc
    43141 gtatctatct gctaattctt ggagtcgtgc tttttgctta tcactaaaag gatactcgga
    43201 cacaatcact ttctttcctg cttccatata caaatcaagt gctttgtaat agaaaggata
    43261 aactttcgtc ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga tggattctgc
    43321 atagtattcc ttgatttcat cgagcgtgat ggcatacata tctggaaatc gttcccgtag
    43381 tttattcata agatatgttt ttcctgtacc aggactacca gccagaagaa tgaaatactt
    43441 tttcatctca tttatttcct aagttaattg gactttccaa gttcataaac agtccaatct
    43501 tatgcttgac aatttctttt actgcttcat ttgctggaat cacattatgg cgaagagact
    43561 tgttgatttc tgtttcacta aaggtttctt tagctacttt cgtccagttg actaaaagct
    43621 ccgttccaac attgaatttg cggatcccgt tattgatcaa ttcaggataa tcttcttctt
    43681 tcacacctgt tcctccatgg ataactaggg ggacatcaac tactgcatcg atttctttca
    43741 gcaagggaat gtttacttct gtttttgatt tgaattgtcc atgattcgta ccgatagcaa
    43801 tagctaatgc atccacgcct gtagcttcta caaatttcac tgcgtcttcc ggtcttgtat
    43861 ataccttgtc attttcagcg acatggattc cttcttctgt accgccgatt gttccaagtt
    43921 ctccttctac ggaaacaccg tgcgcatggg catattctac tactgctttt gtcttcagga
    43981 tattttcttt gaaaggcaga tgggacccat catacatgac agaagtatat ccgctgtcga
    44041 ttgcttcttt gatatcatca aagtctcggg cgtggtctaa gtgcaagact gcatcaacca
    44101 tttcttcttc tgcaatagct gtcattacat tgactaacac atgcatgccg atatatttag
    44161 cggtatcgac acttgtttga ataatgatcg gcgaacccat ttcttttgct gcgcctagca
    44221 tagcaggaag catttctagg ttatgcgtat taaaagctcc aactgtaaaa ttcaactctt
    44281 ctgctgtttt tgttacttct ttcaatgttg tatacatctt ttattgccac cttatctttt
    44341 tatttgtttc ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc cattttgccc
    44401 atgtagtaat cgatcccttg atcatcacca gcacgagcag catctgcacg cttctggtta
    44461 tagaggctca tcagtttttt atacccatct ccgatcgtct gtttattttc caaactcttt
    44521 tctaaatatt ggatggcttg atcgttcaca tacaattcag aaaatttaag tccaacttgt
    44581 tcataaagtt tcgctaattt atgcttatct tgggtagtgg aaatctcttg tttcaattgt
    44641 gtaatctctg attgaatctt ttcggtcgct tctggcgata gggctttttc ttcttttgcc
    44701 agaacttttt tcggttcttt ttcttttttc ttaaaaaatc caaacatcat tttccctact
    44761 ttcaattaaa atgctttcaa aactgggctt aagatccagg catataagag tgctgcggct
    44821 actgtatcta catcggttgc tgtagcattg ttgaatccca tcgaattaaa gatcgttact
    44881 agcaaagcgg gcaataatgt aataaagaaa ccatgagcaa taccgccaat gatcgctcct
    44941 cggcgtccgc caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa gaaattggtt
    45001 agcataccag gtaaaatcat cgctaagccg aacatgggca aagtaaacat ggcaataatc
    45061 gtaccaatcg ttgtcgtaat gaaacctaaa atgacagcat ttgggctata aggaaagaaa
    45121 actggacaat ctaaggcagg gatcgcatca ggaactaatt tcatagctat ccctcgaaat
    45181 gccggcacga tttcacctaa cagtagacgt acaccagcta gcaatacata cacacccact
    45241 acgaattgga tggcttgtaa aaaggcaaac atgatatagt tttgatctcc tgataattca
    45301 gaagcgaatg gttcgcctgc aaataaaaca gtgatgatat atagcgggac catcacgacc
    45361 atgacagaca aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt gatatcttcg
    45421 atcgactttt tattttcgcc tttctcacca aaaagcttag caacaccggc ttcaaataag
    45481 tacccgatcg tacaaaaatg acctaaagca atatcatttg atccagtgat tttacgaatg
    45541 atcggttgcg ctactgccgg catagcaatg gcgaaacaag ctccgataaa accaccgacc
    45601 aagatcaata cgatccctcg cagaccggca aaatagccaa agactgtcgt catcgttgcc
    45661 atccataaaa ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa acgagccaaa
    45721 ataatattga atacaaaaat ggctaagaac gtcagtgcaa tatctctacc taaacccaaa
    45781 tcattcattg cttgaccatt gatagattca atagaaggga tgatcccttg catatgaaat
    45841 ccttcagtaa agattttccc gaaataagtt aaacttccaa cgatgatgct cgaaccggca
    45901 ctcaacactt gaaatcctag gagtgtcttt aacgtgccag agatgacttg tcctgctgat
    45961 ttcttctgca gtagtagacc caacatagca atcaatgcaa tggtgatcga tgcctgagtc
    46021 aaaatatttt cgatgataaa attgataacg cccatg
//
LOCUS       DAZKNH010000011        45902 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000011 DAZKNH010000000
VERSION     DAZKNH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.011"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..1177
                     /locus_tag="ACG8MG_000577"
     CDS             107..1177
                     /locus_tag="ACG8MG_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0374647.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            1192..1860
                     /locus_tag="ACG8MG_000578"
     CDS             1192..1860
                     /locus_tag="ACG8MG_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0374648.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            1880..2458
                     /locus_tag="ACG8MG_000579"
     CDS             1880..2458
                     /locus_tag="ACG8MG_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0374649.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            2859..3770
                     /locus_tag="ACG8MG_000580"
     CDS             2859..3770
                     /locus_tag="ACG8MG_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0374650.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(4148..5065)
                     /gene="rnhC"
                     /locus_tag="ACG8MG_000581"
     CDS             complement(4148..5065)
                     /gene="rnhC"
                     /locus_tag="ACG8MG_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFQ0374651.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            5267..5704
                     /gene="zapA"
                     /locus_tag="ACG8MG_000582"
     CDS             5267..5704
                     /gene="zapA"
                     /locus_tag="ACG8MG_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFQ0374652.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            5762..6310
                     /locus_tag="ACG8MG_000583"
     CDS             5762..6310
                     /locus_tag="ACG8MG_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFQ0374653.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            6425..8785
                     /locus_tag="ACG8MG_000584"
     CDS             6425..8785
                     /locus_tag="ACG8MG_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFQ0374654.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            8872..9186
                     /gene="trxA"
                     /locus_tag="ACG8MG_000585"
     CDS             8872..9186
                     /gene="trxA"
                     /locus_tag="ACG8MG_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFQ0374655.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            9368..11146
                     /gene="uvrC"
                     /locus_tag="ACG8MG_000586"
     CDS             9368..11146
                     /gene="uvrC"
                     /locus_tag="ACG8MG_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFQ0374656.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            11291..12190
                     /locus_tag="ACG8MG_000587"
     CDS             11291..12190
                     /locus_tag="ACG8MG_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0374657.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            12183..13445
                     /locus_tag="ACG8MG_000588"
     CDS             12183..13445
                     /locus_tag="ACG8MG_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0374658.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            13606..15504
                     /locus_tag="ACG8MG_000589"
     CDS             13606..15504
                     /locus_tag="ACG8MG_000589"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFQ0374659.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(15834..16553)
                     /locus_tag="ACG8MG_000590"
     CDS             complement(15834..16553)
                     /locus_tag="ACG8MG_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFQ0374660.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    16743..16790
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            16890..17222
                     /locus_tag="ACG8MG_000591"
     CDS             16890..17222
                     /locus_tag="ACG8MG_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374661.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            17209..19212
                     /locus_tag="ACG8MG_000592"
     CDS             17209..19212
                     /locus_tag="ACG8MG_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFQ0374662.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            19231..19701
                     /locus_tag="ACG8MG_000593"
     CDS             19231..19701
                     /locus_tag="ACG8MG_000593"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFQ0374663.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            19722..20306
                     /locus_tag="ACG8MG_000594"
     CDS             19722..20306
                     /locus_tag="ACG8MG_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFQ0374664.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            20321..21307
                     /locus_tag="ACG8MG_000595"
     CDS             20321..21307
                     /locus_tag="ACG8MG_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFQ0374665.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            21297..21608
                     /locus_tag="ACG8MG_000596"
     CDS             21297..21608
                     /locus_tag="ACG8MG_000596"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFQ0374666.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            21630..23411
                     /locus_tag="ACG8MG_000597"
     CDS             21630..23411
                     /locus_tag="ACG8MG_000597"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFQ0374667.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            23404..24780
                     /locus_tag="ACG8MG_000598"
     CDS             23404..24780
                     /locus_tag="ACG8MG_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFQ0374668.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            24787..25422
                     /locus_tag="ACG8MG_000599"
     CDS             24787..25422
                     /locus_tag="ACG8MG_000599"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFQ0374669.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            25452..26807
                     /locus_tag="ACG8MG_000600"
     CDS             25452..26807
                     /locus_tag="ACG8MG_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0374670.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(26999..28393)
                     /locus_tag="ACG8MG_000601"
     CDS             complement(26999..28393)
                     /locus_tag="ACG8MG_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0374671.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            28485..29456
                     /locus_tag="ACG8MG_000602"
     CDS             28485..29456
                     /locus_tag="ACG8MG_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFQ0374672.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            29453..30235
                     /locus_tag="ACG8MG_000603"
     CDS             29453..30235
                     /locus_tag="ACG8MG_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFQ0374673.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(30404..30775)
                     /locus_tag="ACG8MG_000604"
     CDS             complement(30404..30775)
                     /locus_tag="ACG8MG_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0374674.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            30909..31352
                     /locus_tag="ACG8MG_000605"
     CDS             30909..31352
                     /locus_tag="ACG8MG_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0374675.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(31395..32204)
                     /gene="yidA"
                     /locus_tag="ACG8MG_000606"
     CDS             complement(31395..32204)
                     /gene="yidA"
                     /locus_tag="ACG8MG_000606"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFQ0374676.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(32223..33590)
                     /locus_tag="ACG8MG_000607"
     CDS             complement(32223..33590)
                     /locus_tag="ACG8MG_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0374677.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(33574..34404)
                     /locus_tag="ACG8MG_000608"
     CDS             complement(33574..34404)
                     /locus_tag="ACG8MG_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFQ0374678.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            34567..35004
                     /locus_tag="ACG8MG_000609"
     CDS             34567..35004
                     /locus_tag="ACG8MG_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFQ0374679.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            35080..35700
                     /gene="rpoE"
                     /locus_tag="ACG8MG_000610"
     CDS             35080..35700
                     /gene="rpoE"
                     /locus_tag="ACG8MG_000610"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFQ0374680.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            35889..36395
                     /locus_tag="ACG8MG_000611"
     CDS             35889..36395
                     /locus_tag="ACG8MG_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0374681.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            36412..37311
                     /locus_tag="ACG8MG_000612"
     CDS             36412..37311
                     /locus_tag="ACG8MG_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFQ0374682.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            37398..38282
                     /locus_tag="ACG8MG_000613"
     CDS             37398..38282
                     /locus_tag="ACG8MG_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0374683.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(38474..38545)
                     /locus_tag="ACG8MG_000614"
     tRNA            complement(38474..38545)
                     /locus_tag="ACG8MG_000614"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38510..38512),aa:Arg,seq:ccg)
     gene            38605..39939
                     /gene="rpoN"
                     /locus_tag="ACG8MG_000615"
     CDS             38605..39939
                     /gene="rpoN"
                     /locus_tag="ACG8MG_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFQ0374684.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            40088..41179
                     /locus_tag="ACG8MG_000616"
     CDS             40088..41179
                     /locus_tag="ACG8MG_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFQ0374685.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            41220..42221
                     /gene="gap"
                     /locus_tag="ACG8MG_000617"
     CDS             41220..42221
                     /gene="gap"
                     /locus_tag="ACG8MG_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0374686.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            42331..43521
                     /gene="pgk"
                     /locus_tag="ACG8MG_000618"
     CDS             42331..43521
                     /gene="pgk"
                     /locus_tag="ACG8MG_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFQ0374687.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            43625..44380
                     /gene="tpiA"
                     /locus_tag="ACG8MG_000619"
     CDS             43625..44380
                     /gene="tpiA"
                     /locus_tag="ACG8MG_000619"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFQ0374688.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            44496..45794
                     /gene="eno"
                     /locus_tag="ACG8MG_000620"
     CDS             44496..45794
                     /gene="eno"
                     /locus_tag="ACG8MG_000620"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFQ0374689.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        15232 a         7934 c         9789 g        12947 t
ORIGIN      
        1 tcatataatg aaatagcgga agaatttttt gagcataata ttcgccaaaa atttatcaag
       61 tgataaaatt gttgtataaa atgattattt caggaggagg agttaaatgt ttttagcatt
      121 aaatgaaatt atgcattcaa agttacgtta tgcgttagtt gcaggggtga tgtttttgat
      181 cgcctacctg gtattttttt tgactgggtt agcttatggc ttagctcaag ataatcgaac
      241 agcagtcgac aagtgggagg cagattcgat tgtgctttcg aaagatgcaa attcaaatct
      301 agggatgtcc atgattacta agaagatagc agaagaagtc gaaggtggga aagtggcgta
      361 tcttgcccaa acacctggag tcgtgactag taaagacagt acagaagaag gaaaaatcaa
      421 tgttagcttc ttcggtatcg ataaaaatca attcatcatg cccaatcttg ttgaaggaaa
      481 agcttttgat aacgatgatg aggcagtagg cgatattagt ttgaaagaag aatatggtct
      541 agccatagga gatacagtta agttatctgg tagtgacaag acgtttaaac taactggctt
      601 tacggatcat gccaaattca atgtctcacc agttctctat acaaccatca atgcttacca
      661 agaaatccgc ttcgaaaaag aagatacaag tgaaaatgca cggattaacg caatagttgt
      721 cagaggtaaa atcagtaatt tacctgaaga tttagaacag atcaaaattt ctaaattcat
      781 caatgaatta cctggatata atgcgcaagt attgactttt ggatttatga tcggattttt
      841 gatcgttatt gcggctattg tgatcgggat tttcatttat gtactgacga tgcaaaagat
      901 taatatcttt ggtgtaatga aagcacaagg gatcacagga ggatttattg cgcgttctgt
      961 agtggcacaa acattcattt tatcatttgt tggtattctt cttggtctat taggaacagt
     1021 gggaacatcc cttgttctac ctgatgcggt tccattccaa agcaattggc tgttttttgg
     1081 cgtcattagc ttgttgatgt tagtagtagc tgtgttaggc gcactatttt ctgttcggac
     1141 aatcgtaaaa atcgatccgt tgaaggcaat tggataagaa ggaggaagaa catgaaagca
     1201 atagaattta aagaagtaga aaaaagtttc caagatggcg accaagtagt taaagcattg
     1261 aaagaaacaa ccatgagtat cgataaaggg gaatttgttg caatcatcgg tccttctggt
     1321 tcaggtaaaa gtactttttt gactttggca ggcggattgc agacgcctag cagtggctct
     1381 gtatttatca atgatcaaga attcagtaat caaaaagaaa gcgaacgcgt taaactacgt
     1441 tttaaagaaa ttggctttat tttgcaagct tccaatctgg ttccttttct gacagtaaaa
     1501 aagcagttat tattagtaga taagattaaa catgaacatc gtcaaaaaga agcaaacgaa
     1561 ttatttgagc agcttggcgt ggataagttg atcaacaagt atccagaaga actatctggt
     1621 ggagaacggc aaagggtcgc aatcgctcgt gcattgtaca atgatccgtc cattattcta
     1681 gcagatgaac caactgctag tttggattca gaaaaggcac gagaagtagt cgatatcttg
     1741 gcaaaagaat caaaagacaa aaataaagca acgatcatgg ttactcacga tacacggctg
     1801 attgacaaat gcgatagaat atttatgatg gaagatggtg tattgaaaga aaaagaataa
     1861 ccaaaaagca ggtggaaaaa tggtccatcg atacaacaaa gatacacagc aaaaaatttt
     1921 gaaagaagcg actgacttgt ttatgtccaa gggatatctc ggcacttcaa caagagaaat
     1981 cgcacaaaaa gttgggatca cccagcccaa tctctatcat tatttcggtg ataaagaaaa
     2041 actatatacg gcagttttag aaaaccactt aaaagatgtt ggaaaagctt tgagagaaat
     2101 cgttcaaaca agcgaaacgg gatttcaagt aaccttgaca aaaatggcgc agtttttgat
     2161 tgagacacat ctggtcgatt tgtttctgat gcttcacgat cttgaaagca atctttcaaa
     2221 ggaaacaaga gatcatcttt tctttctatg gaagaaaaat tatcgtgaac cttttgaaga
     2281 aatcttttca gcaaaccaat ctgtgctaag aaatgggatt tcaaaagaaa tcgcagcaag
     2341 gcattttttc ttagtgttag caccttacat cactcggtcg aaaagacctg tggaagaacc
     2401 gctaaatgtg gcgcaattga tcgatttata tttgaatggt gtattaggag aagcgtaaag
     2461 ctgttatata gggagaaaat agttaggtga agaagcaaga tatgacgaag atcgttatat
     2521 cttgcttctt tatataggta gtttttcata aatacaaatt tgttattctc tcatgattat
     2581 cagggtatat ttttatttat tatgtcggtg ttccaggaag tgttttaatt taataacgaa
     2641 gaaattaatt gtttgtagaa tgaaggaaac tatttattgg gtagatgaat caccacgaat
     2701 agtcaaaagg agatttcagg atatgccaaa aatgaaaaaa atcattttag cagtaatgtt
     2761 attttctacg gttatatctt ttacagcccc ttttttgctt acttaagctg attttaagtt
     2821 ttggccctta gtctggatct acgaggtgag agtcaagaat gaaaaaaata attggagctt
     2881 ttgtatttgc aatcattatc attattatct gttttcaaat agatgttttt ggtcaaaaaa
     2941 agatagttgg acaaacggtc agcagtacga acagtacggt tacaaagaca aaggaatcat
     3001 ttgatgatac agaacaagag acattaatca aaatcggaaa aaatgaccgt acactatata
     3061 cagatgactc gctaactacc ccacttgcaa ctataaacgg cggagagctg gcggagtttc
     3121 tttctgaaac aaaaaatgct tatcagatca agacaaatga tggctatact ggatatttag
     3181 ccctcgaaga tggaaccaaa gtcacaaaga atatacaaac aaaaccggaa gagctatctg
     3241 atgcagtgat tgtattagat cctgggcatg gcggaaatga tactggggca ttaagcaatg
     3301 atgaacaaac agaagaaaaa gatataacat taagtacagc cataaaagta aaaaaggcgc
     3361 tagaggatgc tggagcaacg gtttatttga cccatactac agatgaatta gtacagcttg
     3421 gagatatatg cgactacagt gaagaaaaaa aggcagatgt ctttatcagt ttacacgcag
     3481 actctacgga atatgcgaat gaagcaactg gaatcacgac atattattac tatggacaag
     3541 aggaaacatt agcacagaca attgctgact ccttcactga tctgcctctg gattcaagag
     3601 gaatctccac tgggaactat caagttcttc gagaaaatct tcaaccttct attttaatcg
     3661 agatgggtta tatgaataat gatagtgatt taaaggaatt agtaacagac agttatcagc
     3721 agcagattgc tgcaagtttg acacaagcat tgaccactta ttttcagtaa taaataagcc
     3781 cagccaaaca aaaatagctc ctcatatttt tcgtttggcc gggcttatta tcgcagatgc
     3841 aagcttttaa acaccgttta aggaagaggt cgtgagcctg ccgttcaact ctgagtcaca
     3901 aggagcaatt tcaaaaaaca gcttctcatg tttgatgact attgtgactt gtgaccgagg
     3961 agttggcatg agaacaccgt ttaagaaaaa ggttgtgaaa tcaacgtttt gttctgagta
     4021 ttagagaaac aaacggaaaa atagttcctc atattttgcc gtttgttgag ctaataccga
     4081 ggatcagatg attgaacacc gtttattcgg aacaagggac tgtgacatga cttttgtcac
     4141 agttccatta tttttttagt agcttcaaag ctttttctgt attggcaaaa tgaagtttgg
     4201 catatttttc aagcataggc atgccgcctt tctccaatag ttttgccgct acttggtcag
     4261 atttcgttcc agctcctgac ggaagggtga ttccagcttc agctgatgct ttgtctaact
     4321 cttccaaaaa gctggcacga ctgatgatag aagcagcagc aacagctaaa tgatattgtt
     4381 ctcctttagt aacgaagtac agtttttctt gtaattgatt tttttctaaa cgcgcatatt
     4441 ttttaaaatt tgcttccggc gtaaactgat cgatcaaaat cgcttctggt ttttctggct
     4501 cgattttttt tagcagcaaa tggatcgctt gattgtgcaa agcgactttc atccgaacgg
     4561 cattgtattt tggttggatc tcattgtatt tttctggaga taagacaagc agtttatagg
     4621 ggatggtttt ttttaagatg tcagcaagct ggatgatttg gctatctttt aactcttttg
     4681 aatctctaac accaagttta cgtaattctg caagctgatc acgtttcaca taagcagcac
     4741 atactgttag cggaccgaaa tagctgccat ttccgacctc atcactacct aaaacggata
     4801 attgactgat attttttggc aaagaagagg ctgtttttgc tggcttggct tgtttatcct
     4861 gtgcctcccc ccatttatca gcttctattg ctgcattcgt accttgaaac atcacttttc
     4921 cggatgtata agcggtaatg gtcgtcccgt ctttttttgc agcaaagcta gtataaggaa
     4981 tctgtttatt taacaaataa gagcgatagt atgctttcat ttgactaagt tggtcgtttg
     5041 ttgctttgat aatgacgttg ctcataaaat tctcctcgtt tttatacata aaactatacc
     5101 gcaactcacg acttttcgtt ttctaagaga ttgcgagaca tagcattttt ttaaagttta
     5161 tcatataata gttaagtatg tgaaaaatat ttgtggagaa cggtgaaaga accaacttca
     5221 tggtacacta aataaaactt tcatcaaata ggaggaaatg attcgtatgg cgcacgaaaa
     5281 aacacgctac aaagcggtga ttgccaacca aacatatact attatcggac gggaaaccaa
     5341 gcatcatatg gatatcgtaa cgaagctgat caatgaacag ttagcagaat tgaaacaatt
     5401 atcaccacaa atggataatg aacaagcagc aatcttgatg gctgtcaatg cgctttcgga
     5461 tcaattgaag aaacaagaaa gaatattgga gctggaagaa gaaacagcag aattaaagaa
     5521 aaaaatgatc aaatttacag agcttgaaaa tcgtgtgaaa cgcattgaag caatcgagaa
     5581 cgaagctcgg gaagtattga aagaaaatgg tcaagcagat cataccatta aaaatcatgt
     5641 ggaagcacaa cagattttaa acgagaaacg aaaaggacaa attaaacaaa aatcttctaa
     5701 ttaagatcct tttatcaaaa taagaaaaat ttaaaaaagt tagaagagaa aggaacgtag
     5761 catgctgagt ttgctgattt tatttttatt gctaattgcg tttttttctg gagcacgtcg
     5821 cgggtttgct ttgcaaatca tattcgcgat tggatatgta ttgtcattta ttgctgccca
     5881 gcatttttac aaaccattag caagtcattt ggaattatac attccatacc cagctgtcac
     5941 cccaacatca aagttagcat tttttgatca ggtatttgcc tttcacctag atgaagcttt
     6001 ttatgcagga actgcttttc tgattatttt gctaattggc tggctgctta cccgatttgt
     6061 gggggttttt gtccatggat tgacgtatgt gccgatcttg agacaggtag actggattgc
     6121 aggaggcatc ctgtcactaa tcatggctta cgttactata tttttgattt tgcagttact
     6181 tgcctttgtt ccattagatg tggtccaaaa tcaattcggc gagcatagtt tcgctcgttt
     6241 tatcgttgag agaacgccat ttcttaccaa caagatgtat gagctttggg taacgaatat
     6301 tttgaattaa ggaaaatagt gctggaaaat atttcgtttt ttgtacagag tagtttattt
     6361 aagaaggctg cgtagcgtat gtccggcaca gctctttttt catagtgaaa gataaggtga
     6421 taaaatgaat aaccgaatcc ttgaaacatt ggaatttgaa aaagtcaaac agatggtcag
     6481 acaatttgta gtgactgcac aaggaaaaga agaactggca gaattggtac cagtgagtga
     6541 gaaacaaacg atcacaaact ggcttcaaga aactgaggat ggattgaaag tgcaacgtct
     6601 gcgaggcggg atccctatac ctaaaattga aaatatccgt ccccatatga aacggattga
     6661 gatcggagca gacttgaatg gagtggagct tgcacaagtt tccagagtat tgtctacgac
     6721 aagcgaattg aatcgtttca ttgatgatct ttctgacagt gaaatcgaat ttgcacgttt
     6781 gtatatgtgg gcagatcagt tagtcacgat ccctgttttg agccgccgtt taaaggaagc
     6841 aatcgatgaa gacggacgcg tgactgatga cgcttctcct gagttaaaga gcataagaca
     6901 gaacattcgg cgaagtgaac aagctgttcg tgaacagtta gatgggatcg tccgtgggaa
     6961 aaatgcaaag tatttaagtg atgcgatcat caccatgcga aatgaccgct acgttatccc
     7021 agtcaaacaa gaataccgcg gtgtatttgg cggtgtagtc cacgatcaaa gtgcttcagg
     7081 tcagacttta tttattgaac caaaacaagt ggtggatttg aataatcgtt tgcgtcaata
     7141 ccaaatcgct gaacgaaatg agattcaacg tattttaagt gaactttctg ctgaacttgt
     7201 tccccatcgt caagaaatca tccacaatac ttatgtgatt ggtaaaatgg atctgatgaa
     7261 tgcaaaagca cgatttggta aagaagtaaa agccatcgtg ccgggaatca gtgaagacaa
     7321 tcatgtcgtc ttgaaacaag caagacatcc actgatcgat caggaaaaag tagtttctaa
     7381 tgacatcaca atcggaaaag actatcaagc catcgtcatt acaggaccta acacaggtgg
     7441 gaaaacgatc acgttgaaga ctttaggtct gttacaactg atggggcaag caggattacc
     7501 gattcctgca ggtgaagaaa gtcaaatcgg tatttttgag gaagtatttg cggatatagg
     7561 agacgaacag tcgattgagc aaagtctatc gaccttttct tctcatatga cgaacactgt
     7621 agatatttta tcaaaagtca atgaaaaaag tctggtatta tttgacgagc tcggtgcagg
     7681 aacagatccg caagaaggtg ctgctctagc aatcgcaatc ttagatgatc ttggtaaaaa
     7741 gtctgcttat gtgatggcaa caacgcatta tcctgaattg aaagtttatg gatataatcg
     7801 ggcaaatacg attaatgcca gcatggagtt tgatgtggat acattaagtc caacgtatcg
     7861 tttgctgatt ggcgttcctg gacgaagcaa tgcgtttgaa atctctagcc gattaggtct
     7921 tgatacagaa gttattgatg aagcgaagca gttgatgaat gatgagagcc aagatttaaa
     7981 cgaaatgatc acagatttag agaatcgccg gaaaatggca gagaccgaat atctggaaat
     8041 gcgtcatttt gtttctgagg cacaagaact tcacgatgat ttgaaagaag cttacagcta
     8101 tttctttgaa gaacgtgaaa aagaaatgga aaaagcaaag aaaaaagcaa atgaagtcgt
     8161 gtcagaggcg gaagaaaaag cagaaaaaat cattgctgat attcgtaaaa tgcaacagca
     8221 gattgggcaa ggaaatgtga aagagcatca gttgatcgat gcaaagaccc aattagcaaa
     8281 tctacatcaa gaagaaactt tgaagaaaaa taaagtattg aagaaggcga aagaacaaaa
     8341 aacgttgaaa ccgggagacg aagtgttggt tacgacttac ggccaaagag ggacgctttt
     8401 acgtaaaaac gggaatcagt ggcaagtaga aatcggtata ttaaaaatga atgtgtccga
     8461 agacgagctg acgcctgttg ctcctcaaaa agagccgaca caacgagtta tccatgctgt
     8521 acgatctgaa tctagtagtc acgtgccgaa tcaattggac cttcgaggaa aacgatacga
     8581 agaagcgcta agtgaggtag atcaatattt ggattctgcc attctagctg gttatcctca
     8641 agtgactatt gtccacggaa aaggaacagg cgcacttcgc aagggaatta ctgattatct
     8701 taaaaaccat cggagcgtca aaagctttga gtttgcaccg gcaaaccaag gcggaaatgg
     8761 tgcgacaatt gttaaattta agtaaagctt actaaaggta agcagatggt tcgctatcca
     8821 agtatagaaa tgctatacta gtcatagatt ttaaaaatgg aggtctttga tatgtcacaa
     8881 gtaattacag ataagacatt taatgaagaa acagataaag gacttgtatt gattgatttt
     8941 tgggcaactt ggtgtggtcc atgtcgtatg caagcaccga ttttggatca gttggaacaa
     9001 gaatatgatg aagaagaatt cagaattgct aaaatggatg tggatgaaaa tccagaaact
     9061 cctcaacaat tcggtatcat gagcattcca acattaatgt tgaaaaaaga tggtcaagta
     9121 gtagaaaaag ctgttggtgt tcattcaaaa gaacaactac gtcaaatgat cgatcaatat
     9181 ttataaaaac ttacagcaaa aataagctga gggtgggact gaatccagtt ccgtcctcgg
     9241 tttttttgcg gttgagttaa ggttattcaa attaatattt tttgagtata attacgttgt
     9301 caggctggat tgtttttttg acagacatgc tataataact ttaattgaat atgaagggga
     9361 gtaccagatg aacgaacgaa tcaaaaacaa attagcttta cttcctgatc agcccggctg
     9421 ttacttgatg aaagataaaa atggaacgat catttatgtc ggcaaagcca aaatattaaa
     9481 aaatcgtgtc cgctcatatt ttactggtag ccataatacg aaaacggaac ggcttgttag
     9541 tgaaatcgaa gactttgaat acatcgttac agaatcgaat atcgaagcat tgctcttaga
     9601 gatcaatctg ataaagaaaa atgatcctaa atacaacatt atgttaaagg atgataaaac
     9661 ctatcctttc cttaagataa cgaatgaaaa atatccccgt ttggtgatta ctcgtaaggt
     9721 actaaaagac aaagcactgt attttggacc atatcccgat gtcggtgcgg cgaatgagac
     9781 aaagaagatc ctcgatcgat tatttcctct gagaaaatgc aaacctagtc agacgaagga
     9841 gccttgtctt tactatcatt tgggacaatg cctgtgtcct tattatttcg acgtagatcc
     9901 gaaagtctat acgaaaatcg ttgatgaagt gaaaaacttt ttaaacggag gacacgaaaa
     9961 aatcgagtca gagatccatc agaaaatgga aaaagctgct gaaaacatgg agtttgaaaa
    10021 agctgcagaa taccgagacc agatccgtgc cattgagaca atcatgactt gccaaaaaat
    10081 gacaaatact gatttactgg atcgagatgt atttggttat gcgattgata aaggatggat
    10141 gtgtgtccaa gtgtttttcg taagacaagg aaagctaatt gaacgagatg tttcaatgtt
    10201 tcctttctat aacgaagcag aagaagattt cttgacttac ctcggtcagt tttatcagga
    10261 aaatgagcat ttcattccta aagaagtctt gattcctgat acgatcgaca agcccagtgt
    10321 agaagctttg ttagctacga aagtgctgca accaaaacga ggagaaaaga aaaaattagt
    10381 tgaattggcg agtaagaatg cctcagttgc tttgaatgaa cggtttgatc tgattgccag
    10441 aaaacaggaa cggacgatcg gagcagttga acgtttagga aacgcgatga atatcccgac
    10501 cccaatacgt atcgagtctt ttgataactc taatatcatg gggacgaatc ctgtggctgc
    10561 gatggtcgta ttcattgatg gaaaaccggc aagaaaagaa tatcggaaat ataaaataaa
    10621 aacagtcaac ggaccggatg attatgcatc aatgagagaa gtcatctatc gcagatattc
    10681 gcgagtgatt cgcgaagggt tgcctttacc tgatttgatc ttgatcgatg gaggaaaagg
    10741 acaagtagat gtggcaaaag atgtcttaga aaatcaatta ggaatcgata ttcctattgc
    10801 tggcatggta aaaaacgata aacataaaac aaatgagttg ctattcggta cagatttggc
    10861 tgtagtaccg cttgagcgaa attcaccaga gtttttctta ctccagcgaa tccaagatga
    10921 agtccatcga ttcgcaatca ctttccatcg gcagctacgc agtaaaaaca gttttgcttc
    10981 ccgattagat gaaattgaag ggttaggacc aaaacgaaaa aaaatgctgc ttaaagagtt
    11041 caaatcgttg aaaaatatca cagcagccac gacagaagaa ttacaagaaa tcggactgcc
    11101 gaaaaatgtc gcacaaaacg tatttgataa attacatcag gaatgaggaa agggcacttt
    11161 acaaagaaag taaagtgtcc tatcatttta tgtacaacaa aaagagcgtt tacgttttgt
    11221 tggtaaataa taaatcgctt acgtctggtg tctaaggata tatgtgattt cttgaagaag
    11281 gagtgaagaa atgttagaag tcaaggatct tgttaaaact tttgggaact acacagctgt
    11341 cgatcatgtc tctttccaaa tccccgatgg aaaaatcatg gggctaattg gccaaaacgg
    11401 ggctgggaag acaacgactt ttcgtttgat cttggatttt ttgacacaag accaaggaga
    11461 agtgttatgg aatggccatc atcttgggga gaaagattat gatgtcatcg gttatcttcc
    11521 ggaggaacgg ggactttatc cgaaagtaac gattcaagac caactgcttt attttgcaga
    11581 acttagaggg aaatcaagaa aagagattga accaaaaatc gacttttgga tggaaaaatt
    11641 tcaggtaaaa gggaaaaaga cggataaagt aaaatctttg tctaaaggaa atcagcaaaa
    11701 agtccagctg atcgccacgt taatccatga acctaaattg atcattttgg atgagccatt
    11761 cagcgggtta gatccggtaa atgctgaatt gctaaaagat ggaatattgg aattgaaaaa
    11821 acatggttca tgtgtcattt tttctagcca caatatggat aatgtagaaa aaatctgcga
    11881 tcatttgatc atgttgcgaa acggaaaaat ggttttgaat ggaaaagtac atgagattcg
    11941 tgaatcattt ggccgtacga agttattctt ggaatctggt ttaagccaac aggaagtaga
    12001 agaaatcgaa ggcgtgaaaa aagtgatcct tcatgaagac aatacgttag agattacctt
    12061 agaagatcct gaagctggga aagaaatctt tactcgtgcg actcagttcg gctacattcc
    12121 tatgtttaac cagcaaccgc caactttgga agagattttc aaattgaaag caggtgaaat
    12181 cgatgcgtaa gttttggatt attaccaaag atgtctattt aaaaaatgtt aagtcgattt
    12241 ctttcttgat catgatatta gtccctttta tcatgctggg tatcatttat gtagcaggaa
    12301 actttgctca acaaaatagt gatgtcaata aaatcggcgt attggctgat aatgagcaaa
    12361 tcagtcagca aatgagccaa gtcaaaacag atgattttga gttcaaagca gttgattctg
    12421 aagaaaaagc acaaaaagag ctttcggaag aaaaaatcga tgcgtattta gtagtctcaa
    12481 ctgagaatga taagataaat gggatgcttt atagcgaaaa ttcattagga caaagtacac
    12541 aactactgat acaacaacaa ctgactggct tgcaatcgat gcttcgagcg agtcaattag
    12601 gcatttcacc agaagaagta gctagcttga gccaacaagc aagtttgact cgacaaaaag
    12661 tcaattttga ttcaaaagga aaaatgacag tcggagaaga taactcgaca gtccaatacg
    12721 tcgtaagtta tgtagcaacg attgtcttgt tcattattat tttgacgtat gcgcagatca
    12781 ttgcacaaga gatcgcttct gaaaaaggca cgcggatcat ggaagtgatc ctgtccagta
    12841 cgactgcaca aaagcatttt tacggaaaat taacaggtgt tttacttgtt gcattaacgc
    12901 aaatggcatt atacggtatt atttttggta taggatacag tcaattcaaa gatatggaaa
    12961 tggtcaaaaa tatattagac ggtatctcat tggataactt gattggacca ttcttgtggt
    13021 attctcttct attcatgatc tttggcattt tgattttttc tgttttagca gctttatgtg
    13081 gctctttggt caacaaagca gaagatacag caaaagcaat tttgccagtg acttatctat
    13141 ctctaggcgg atacatgctt ggattgatac taggagcttc agatccaaac aatatcgtta
    13201 ttcggatcac ttcgtatatt ccattcttat cttcatatat tatgccgatt cgcttagcaa
    13261 atgagacagt cggtatgggt ggagcattga tttccttgtt ggtacttgta gccgtaacat
    13321 tcttattgat gatcttgtct gcaaacatgt acaaatcgaa tgtactcgtt tatagcgaag
    13381 gcggattatg gagttcattg aaacaatcga tttcaattat gaggaatgaa cgaaaaaaag
    13441 cataaaacaa tcctaaaata cagaaggaag ctggacatct attgttccca gcttcttttt
    13501 gtatagacct attttattta ttttactata ttggatatat attcattgaa aatagtgtat
    13561 aacaacttta taatattgag tataccaatt tttggaggcg ttttaatgga ccgttattta
    13621 gatttacttt cagaaaaatt cccctcaacg gaggatgtca tcacggaaat catcaatctc
    13681 gaagcaattt tgcagcttcc aaaaggaaca gagttatttt tgagtgatat acatggcgaa
    13741 tttcctgctt ttgatcatat tctgcgtatc ggttcaggaa atcttaaaga aaagatcaaa
    13801 gacctttttt ctgaacaaat gacggaaacg gatatcagcc aattcactat ttttacggct
    13861 tatccagaat atgcattaac gactgattgg tacaaagaac aagataaatc gcagctgatc
    13921 catcatttga tcgatctttt acggtttacg acagtcaaat atacccggtc aaaagttcga
    13981 aaaggattgc cgaaagaata tagctatatc attgaggaac tcttgtatat cgatgaccgt
    14041 atcaatggaa aaaaagacta tgtaaaaaaa atcattgaac aacttgtttt gatgaaggaa
    14101 gaagaaaaat ttttaacgaa acttgcacag acgatccaaa aatctgtcat cgatcatttg
    14161 catatcgttg gagatatttt tgaccgagga actcaagcag ccaaagtgat ggatcgaatc
    14221 atggattttt cttctgtaga tatccaatgg ggaaatcatg atattttgtg gattggtgcg
    14281 tattgcggct ctgaggcatg tttactgaca cttttacgga ttgcagcacg ctataattat
    14341 ttatttgaat tagagaaaga atatgggttg aatttacgtc cattgttttc ttttgctcat
    14401 caaacttatc agaagaatcc agtatttgcg cctaaaaaca gtaaaaatct atccgaaaga
    14461 gagcaagaag aattagaaaa aatccatcag gcactttcta tccttcaatt caaatcagaa
    14521 catcagttat tggatcgtcg acctgaattt aaaatggacg atcgtaaatt gctggaaaaa
    14581 atcgactatg aagcatcaac gattgattta gaaggtcagc tctatgagtt gaaaggaact
    14641 tgttttcaaa cgatccagcc agatgatcca aaaaaattct tggaagagga acaaaaagtg
    14701 attgatagtt tgatgtattc attccagcac tctttgagaa tgcaaaaaca tatgacgttt
    14761 ttgatcgaaa aagggggaat gtatctcacg aacaatcagc atgtgctgtt tcatggctgt
    14821 atacctgtag acgagaaggg acagctctta gcatttgaat tagaccaatc ttatcgcgga
    14881 aaagaactgc tgggcttttt tgaaaaacag ataactgaaa gcgcaaaaga tctgactgca
    14941 aaagaagatt tagcaaccga tttgatttgg tatgcgtgga gtgggccatt ttcgccttta
    15001 ttcggcaaga gtaaaatggc gacttttgaa cgttattttc tcacagagcc tcatacgcac
    15061 gtcgagaaaa gcaatcctta ttatcacttg cgagatgaag agtggttcag tgaaaaaatt
    15121 ttagcagaat tcgacacaaa agaagaaggg tcagcaatca ttaatgggca cacgccagta
    15181 aaagtaaaaa aaggggagtc accgatcaaa gcaaatggaa aagtatttgt cattgacgga
    15241 ggtttatcca aagcttatca aaaaacgaca ggaattgccg gttattcact attatgcaat
    15301 tcttatggtt tccaaatcgt gacacattat ccgtttttgc ctattgaaca acaatttgca
    15361 aaaaaatccg accaaacaaa tgtgaaaaaa gtaatcgaac agcaactacc acgcaaactc
    15421 aatcgtgata caacaaaagg tatggaactg cagcagcaga ttacccagct caaaaggctg
    15481 ttggactatc gaaaggacga ttgagaagaa ttccagtgaa cattgttcca tagactggag
    15541 actgagacaa aagtcgtgtc gcagtctctt tttccggata aacggtgttc ttctgccaac
    15601 tcctcggtca caagtcacac tattcaacaa acatgaaaag ctgttttttg aaattgctcc
    15661 ttgtgactca gagttgaacg gcaggctcac gacctcttgg tataaacggt gttcaaaagc
    15721 cagtttctcg gtattaagcc aaatttatgc aaaaatgtga aaagccattt tcacaaaatt
    15781 tttcttaata ctcgaaacta aacagctttt tcacaacccc ctcagaaagt ctattaaaat
    15841 tcattcacaa agaaatcgaa taagcgctgt tcagatggat gtacacggtg ggtaagttcc
    15901 ggatgccatt gaacaccgag gattttatgc atatcatctt tgctttgaac cgcttcaacg
    15961 agtccatcgt tagaaaaagc gatggctttt aataaaggtg aaagatcttt gactgcctga
    16021 tgatggtaag aattcacaaa atatggatcg gggagaaggt tgcttaacaa actgtctgaa
    16081 acaatcttga cttcgtgagt cgtaaattgc ggggcagtgg gctgttgttc atgttttact
    16141 gaccactttg gatataatga aagatcttga taaagtgtac cttccaaaac gacattcaat
    16201 agttggagtc cccggcagac tgcaaaaatt ggttttcttt gtttcaatgc ttccaagatc
    16261 agtgcctgtt caaattggtc tcgttgaata tttgtttccg ttagctttgg atgaggatct
    16321 tccatgtaaa gttgtggact gacatcttga ccacctgcaa gaataagttt gtcgatttgt
    16381 ccaatcagtt gaggagctga atctggagac atgactggca gaataagagg caatccttga
    16441 gcatctaaaa ctgcatccac gaatccttgc ggtgtatagc tcacttgatt tccttggaaa
    16501 gtatctgttg ctcgaatcag ttgatttccg gcaatgccga tgattggttt catttgtatt
    16561 ccccctaaag tagacttatt gtaacaagga tttaaaaaaa attaaactat gtaagacttg
    16621 cccctatact tatctgttct tgctgtgtta ctatctgtaa agagaaattt cgtgtgaaat
    16681 tcatgacgat agttgaaaat attgcttaat ttgttataat aaaaagtaag cagaaataaa
    16741 aaggttgggc gtaagcttca aggatctagt tcttcaaggg gtgagaagga cattttattg
    16801 aaggggcctg cctatactaa gttatcttta gaggtacata ctttcaatga aaaaagaaag
    16861 catgtacctc ttttttaaat cgaaaggaag tgaagcaagt gttagaagct ttggaacaaa
    16921 tccgaaaagc agaagaagaa aatgaaaagc gtgctcaaac actgcggcag cagctgaaga
    16981 tctatgcaac agaaaaagag caggaattgg aaacgatcca aacaaactat cgacaacaac
    17041 taaaacagtt ggtggaagaa aaagaacaat ttgaaataca agaattagaa aaagaaaaaa
    17101 tgaaattgaa agcagaagca gacaaggtgc aagcaacttt gaaagatcaa tatcaaaagc
    17161 atcagagtca agcgatcggt gcgatcattg aaagggtgaa agaaacttat ggccgtcact
    17221 aagatggaaa aagtgaccct catttcagat aaaagaaatc aggagaggat tttacaagca
    17281 gtacaaggaa tccaaaacgt tgaaatccgg gatctgtatc aaaatagaac aaacaaccag
    17341 tgggtggaga agtattttcc agataccacg attattgata aagaagcaaa attaagcgct
    17401 ttaggtaatc ggttgaaaga catccaagaa gctatccaat tcatacaaca ccacggcgat
    17461 agccgtcaaa agaaactcca tttgaaacgt caagaacttt ccttacaaga tttggaagcc
    17521 aattattccg aagaaaattt cataaataaa ttgaaagaag tccttgattt aaaagaaaag
    17581 tgggaacaac taactgaaga acgtgatcgt ttagaagaag aggaagaatg gcttcttcat
    17641 tggcaacagc tggatattgc tccaagtatg tatgcaagta agacgaccat ttttgagatg
    17701 gggactgtga gtgcagcaaa ttttgaaagt ttcaaggaag aactgagtaa gcttgatgac
    17761 gtttatatgg aagaaataga ttatacgcca aaacaagttt atctttcgta tgttgcttta
    17821 aaaaaatcag ttgctgttat agaagaaatt gctgggaaat acgggttcaa taaagaaacg
    17881 tatccttatg aacaaaatcc taaagaaaaa ctgcaagaga ccaagcagca attatccatt
    17941 gttcacgagc agcaaaagaa gataacgaca gcgttaggtc agtgcagtgg ctatatccaa
    18001 gaatttgaat gggtagaaga agtcacactt gccgttgcag aacgagaaaa aataaaagaa
    18061 cggtttatcc atgcgactta tctgatcgtt ttgcaaggtt gggtagatgc agaagaaaaa
    18121 cagaatttgc tgcatgtgtt aaacgaaacc gtatcagaag aagatatata cgtcagtttc
    18181 gaaacaccaa actcagaaga gattcagcag gaagtcccta ctaaattgaa aaaccatccg
    18241 cttgtcgaac cttttgaatt gctgactgaa atgtacagtc tgccaaaata tgaagaagta
    18301 gatccaacac cttggatgac accattttat cttgtcttct tcgggatgat ggtcgcagat
    18361 gttggttatg gtttgctgat gctcattggg acaatactag ctcaaaaact acttgttcta
    18421 ccacgcggga tgaaacgttt cgtaaaattc tttgagattc tctcgatacc atcgatcatc
    18481 tggggattga tctacagttc atttttcgga cagtcattac caaaagaact gtttggcatc
    18541 cgcttgcctt tcccaatcct atctacgacg gatgacgtca atacgatttt gattttatcc
    18601 gtgattttcg gtctgataca gattttggtt ggattatttg tggcagcaaa ggaaaatatc
    18661 aggagaaaag attacttgag tgctgtcagt gatggatttg cctggcaagg gatcctaatt
    18721 gggatcgtta ttgcgttggt tggtgccatg ttgataaaaa atacagcatt tgtttacctt
    18781 ggcgctgggc tatcgatcat tgctgcctta tgtatcttga tcgttccgat tatccaaacg
    18841 ccgtctaaag tcaaaggtgc tgccaaaggt gcgtataatc tttacggact gaccggctat
    18901 atcggtgatt tagtcagtta tacacgtttg atggcattgg gtatttcagg cggaagtatt
    18961 ggagcggcat tcaatatgct agttgctttc atgccgccag ctgctcgatt cagtgtaggg
    19021 atccttttga tcatcgcttt acacgcactg aatatgtttt tgacactgct aagtgcctat
    19081 gtccatggcg cacgattgca gtatgtcgaa ttttttggga agttttatac aggaggcgga
    19141 cgtgcctttg atccgctgaa aacggcggaa aaatacgtca acatcaatca caagaaaaaa
    19201 aacaataaat aattggagga tttttatata atgatggatt acttaattag tcaaaatggt
    19261 ggaatggtat ttgcggtatt agcgatggca acagcgacga ttttttcagg gatcggttca
    19321 gctaaaggtg ttggaatgac aggtgaggca gcagcagcat tgacaacaag tcaacccgaa
    19381 aaattcgggc aggctctgat tctgcaattg cttccaggca cgcaaggttt gtatggattc
    19441 gttattgcct ttttgatttt tatcaactta ggaaatgaca tgtcagttgt acaaggactg
    19501 aactttctag gtgcttctct gccgattgct tttacaggct tgttttcagg tattgcccaa
    19561 ggaaaagtcg cagcggcagg tattcaaatt ttagcgaaaa aacctgaaca tgctacaaaa
    19621 ggaatcattt ttgctgcgat ggttgaaaca tacgcgattt taggtttcgt tatttccttc
    19681 ctattagtgt tgaatgccta agcataaagg aggaaaagca gatgaacgcc attgaaaaaa
    19741 tcatctctca gatgaacgaa gcagcagagc aggaaagagc ggcgttagaa caagaagagc
    19801 gaatgaagat cgatcaaaac tttgaacaaa aacgtacgca agttgaaaca gaacatcaaa
    19861 aacaaaaaga aaaacaaatc gaattactcg aaaaaaaata ccgtcagcta aggaatcgcc
    19921 agcaagtaga agtacgacaa gaaaatttga atgcgaagca ggaattttta agacgattgt
    19981 ttgcagatgc ggtaactgaa atggaaaatt gggatgaatc tgaacaaatc cagtttatca
    20041 aaaatgcatt gtattcatta cctttgacag gaaaagtagc attcattgct ggagaaaaat
    20101 cagcagccta tctttctcag acgttgttgg atgaatggaa caatgaactt ccctttatga
    20161 tggttttaag tgatgaaact gttgctgatc aagcgggttt tctgataaac gatcaaggag
    20221 ttcagtataa ttttcttttc agcagccttg tacaagatat ccaaggaacg atgagttttg
    20281 agattgccaa tcaattattc gaataaggag ggatcagtgt atggaatacc acgaactaaa
    20341 tcccttgatt cgcggaagag agcttgaact tttgtccaat gagacatttg aacggatgat
    20401 tcaaacaaat tctgtcgaag cacttggtga aatattgaaa tccacgattt acagtccata
    20461 tatttatgac ggatatgaat atgacttcga acagaatctt ttaaaggagc agagccgtct
    20521 atttcgctgg ttaaaggaat cagcaccgga gcctgaaatc gtttggatct atacgatgcg
    20581 ttatactttc cataatctga aggttttgac aaaagctgag atgacaaatc aaaatctgga
    20641 ccatttgtat atcgacgatg gattttattc attagaaacc ttgaaggatg ccattcatac
    20701 gcaagtttca aatgaacttc ctgaaagcat tatggacagt atacgagaag tacgtgatta
    20761 ttttgaagag tctaccattc ttcagggaat tgacgtgatt tatgatcgac agtttttgac
    20821 agagcagcga cggcttgccg ataagctagg atatcaagaa ttgctgacag aagtcattgc
    20881 ttttattgac ttgaccaata tcacaacgat ggctcgagga atattgcaga aaagaacata
    20941 tgggttcatg acgacggtac tttccagctc gggaagtatc gaaaaagaag tatttttgaa
    21001 ctttgtagat aaagatatgg aagattacac gaattttcta ttagagacag attatgctga
    21061 ttttctgcgc cctgtcatcc gtgagggaaa tattgatctc gtaaagcttg aacagttgaa
    21121 agatgattat ctaagttcat tgtatcaaga agcacaaacc caggctttcg gaccactgcc
    21181 actactagcc tttttgaacg caaaagaggt agaaagcaaa aaccttcgtt tactggttat
    21241 cggcaaaaga agccatttcg aatcagaaac aatcagaaag cgggtgagac aagtctatga
    21301 cgcataagat cggtgtagtt ggcgacaaag attctgtttt gccattcaaa ttgtttggct
    21361 ttgatgttcg ttatggtaca accaagcaaa ccgttcgtca ggcaattgaa gaaatggcaa
    21421 agaatgagta tggcgtgatt tatgtcacag agcaatgtgc agcattgagc ccagaaacga
    21481 tcgaacgtta caaggaacaa atgatacctg ctatcgtatt gattcccaat catcaaggat
    21541 cattagggat agggctggaa gaaattcaaa aaaacgtaga aaaagcagta ggtcaaaata
    21601 tcttatagta tgaaaaggag atagaaattt tgcaaattgg aaaaatcata aaagtctccg
    21661 gacctcttgt aatggctgag aacatgtctg atgccagtat ccaagacatg tgtctagtgg
    21721 gagatctagg agttatcgga gaaattatcg agatgcgtgg cgatgtggcg tctattcaag
    21781 tatatgaaga gacttctgga attggtccag gcgaaccggt tcgctctact ggtgaagctt
    21841 tatctgttga attaggacca gggatcattt cacaaatgtt tgacggaatc caacggccat
    21901 tagatacctt tatggaaatc acacaaagta atttcttagg gcgcggagtt caattgcccg
    21961 cattggacca tgagaaaaaa tggtggttcg aaccaacagt agaagccgga gaaacggtga
    22021 gtgctggaga tgttatcggg atcgtagaag aaacaaaagt tataaaacat aaaatcatgg
    22081 tgccaaatgg gataaaagga acgatcaaaa caatcgaaaa aggttcattc actattgatg
    22141 aaacagtatg tgtcattgaa accgaaaatg gtgacaaaaa tttgacgatg cttcaaaaat
    22201 ggccagtaag acgtagtcgg ccaatcaaag aaaaattaaa cccggatgca cccatgatca
    22261 ctggacagcg agtgattgat acattcttcc cagtgacaaa aggaggagca gcagctgtac
    22321 ctggtccttt tggtgcaggg aaaacagtcg ttcagcatca aattgcaaaa tgggccgatg
    22381 tggacatggt tgtctatgtg ggatgtggcg agcgtggaaa tgagatgacg gatgttttaa
    22441 atgaattccc tgaattgatt gaccctaata caggagaatc gttgatggaa cgtacggtat
    22501 tgattgctaa tacatcaaac atgccagtag cagctcgaga agcttctatc tatacgggaa
    22561 tcacaattgc ggaatatttc cgtgacatgg gttatgacgt tgctatcatg gcagactcca
    22621 cttcacgatg ggcagaagca cttcgtgaga tgagcggacg tttggaagaa atgcctggtg
    22681 atgaaggtta tccagcttat cttggttcac ggcttgcgga atactatgaa cgatctggaa
    22741 gagtcattgc attaggatca gaaggtcgcg aaggaagtat aaccgcaatc agtgctgtat
    22801 ccccgtctgg aggggatatt tcggaaccag tcacacaaaa tacattgcgg gtcgtaaaag
    22861 tattctgggg tcttgattcc agtttagccc aaaaaagaca ttttccttcg atcgattgga
    22921 tacaaagcta ttctctttat tcaacagaag tagggaagta tatggatcat atccttcaag
    22981 ctgactgggc atccatggtc accgagggca tgcgtatttt gcaggaagaa cagcagctga
    23041 atgaaatcgt gcgtttggta gggattgatt ccctttcaga taatgaccgt ctgacattag
    23101 aagtggcaaa atccattcgt gaagattatt tgcagcaaaa tgcttttgat gatgtcgata
    23161 cattcacttc aagagagaaa caattcaaaa tgttgaagat gatcttgacc tttggtgaag
    23221 aaggacgccg tgcgttgtcg ttaggtgctt atttaagtga gatcatggac ggtaccgtat
    23281 cgattcgtga aaggatcgga cgaagcaaat atatcccgga aacagaatta gaaaaactag
    23341 atcaaattat tggtgaaatc aagcaaacga tgcaaacgat cgtttcagaa ggaggcatga
    23401 gcaatgatta aagaatatcg tacgatcggt gaagtcgtcg gtccattgat ggcggttgaa
    23461 aaagttgctg gtgtcaaata tgaagaatta atcgaagtcc gtatgcaaaa tggcgagatt
    23521 cgtcgtggac aagtattgga agtccaagaa gataaagcaa tggtccaaat attcgaagga
    23581 acaagtggta tcaatttacg tgactcttct gtacgatttt taggccatcc tttagaacta
    23641 ggtgtatcgg aagacatgat cggtcgagta ttcgatggtt tgggacgacc gaaagataac
    23701 ggaccagaga tcctgcctga aaagcatttg gatattaacg gagaagtaat caatccagta
    23761 gctcgggatt atccagatga gtttatccaa acaggtatct ctgcaatcga tcatttgaat
    23821 acattagttc gtgggcaaaa attaccagta ttttctggct ctggtcttcc tcataaagaa
    23881 ttagctgctc agatcgcgcg acaagcaacg gtactcgatt catcagatga tttcgccgta
    23941 gtctttgctg ctattggaat cacttttgaa gaagctgaat ttttcatgga agattttcgt
    24001 caaacgggcg caatcgaccg ttctgtcatg tttatgaatt tagcaaacga ccctgcaatc
    24061 gaacgtatcg ctactcctcg tatggctttg actgctgcag aatatcttgc ttatgaaaag
    24121 ggaatgcatg ttttggttat catgacagac atgaccaact atgcagaggc attgcgagag
    24181 atttctgcag cacggcgaga agttccagga agaagaggat atcctgggta tctttacacg
    24241 aacttagcta ctttattcga acgagcagga agaatccgcg gactaaaagg atcggttact
    24301 caaattccga ttctaacaat gccagaagac gataaaactc atccaatccc tgacttgact
    24361 ggttatatta cagaaggaca gatcatcttg actagagagc tttacaagag tgggattcag
    24421 ccaccaattg atgtactccc gtctctttcg cggttaaaag ataagggaac tggacccgga
    24481 aagacaagag aagaccatgc agctacgatg aatcagcttt ttgcagccta tgcacaagga
    24541 aaacaggcaa aagaattagc cgtagtttta ggtgaatctg ctttatctga tgtagacaag
    24601 atctatgcga agtttgctga gcggtttgaa aaagaatatg tcaatcaagg attctacacg
    24661 aaccgttcga ttactgaaac acttgatcta gggtgggaac tgctatcgat gttgccaaga
    24721 accgaattaa aacgaatcaa agacgacatg ctggacaaat atctgccaga agggaagtga
    24781 gttctgatgg cacgattgaa tgtgaatccg acccgcatgg aattgactcg attgaaaaaa
    24841 cagttgacaa ctgccacaag agggcataaa ttattaaaag ataagcaaga tgaactgatg
    24901 cgtcaattca tcctgttgat tcgaaagaac aaccagctgc gtcaagaaat ggaagagtct
    24961 atgacagaag caatgtctga ttttgtattg gcaaatgctt cgatcacgga acctttcatc
    25021 gaagaattat ttgttttgcc ggcggaaaac gttgatttgt ctatcgtaga caaaaatatc
    25081 atgagtgtaa aagtacctgt gatgaatttt gaatatgatg aacagctgac agacacacct
    25141 ttggaatacg ggtatttgaa ttccagtgcc gaactggatc ggtcaatcga tcggtttact
    25201 accctgcttc caaaactttt ggaactgaca gaaatcgaaa aaacttgtca actgatggcg
    25261 agtgaaatcg aaaagacacg tcggcgagtc aatgcattag aatatatgac gatccctcaa
    25321 ttagaagaaa cgatctatta tattcgtatg aagctggaag aaaacgagcg cgcagaagta
    25381 acaaggttga tcaaagtaaa aaatatgggt accactgact aagtggtatc tttttttaaa
    25441 agaggaaaac gatgattaaa caaagcaaac aaataaagaa acgtttgccg gctgttcagc
    25501 ttctcgcatt aggatttttt ctcctgattt tagttggcgg cggcctactt accctaccat
    25561 tttttagtaa tagtgggcat gggacaaatt ttatcgatgc gcttttcact gcaacttctg
    25621 ctgtctgtgt aacgggattg acaacattga atacagcaga gcattggaac agtgctggtc
    25681 aatttttgat catgcttttg attgagatcg gcggtcttgg ttttatgatg gttccgattc
    25741 tatgctttgc attgataaag aagaaagtca gtctcagcac acgtattata ttaaaagaag
    25801 cgctgaatct agaagaagta tcaggtgtga tgaagctcat gctttacatc ctaaaattgg
    25861 cctttgtgat ccaattgatt ggtacggttg ctttatcttt tgtatttgtg cctgaatttg
    25921 gctggttgaa aggtatctgg tacagtttgt tccatgcaat ctctagtttt tgtaatgcag
    25981 ggtttgattt gttaggagac agtttagcca atcaccagca aaatgtttat ttgatcatgg
    26041 tggtatctgc tttgatcatt tctggtgggt taggcttcat cgtatggcgt gatttactgg
    26101 aatatcatca gaaaaagaag atgacgattc attcaaaaat cgcattaacc gttacaggta
    26161 cccttttatt aggcggattc gttgtgttct ttttcacaga gcgcaatggg gcaacgctag
    26221 tggaagggaa tatgtttcaa cgattggcaa acaccttttt tatgagtgtg acacctagaa
    26281 cggctggata ttattcagtt gattacctcc atatgagcca tgcaggtctg attttaacga
    26341 tgtttttgat gtacatcggc ggaacatctg ggtcgacggc cggcggattg aaaacaacta
    26401 cgttaggtgt gttgctgatc cagatgcgtt cgatgttgaa aggacgtacg agagcggaag
    26461 catttggccg taccatccga caaggcgcag tgttgcgggc acttacttta ttttttgtta
    26521 cgttgacgtt gtgtgtagtc gccatcatga ttttatcggt gactgaaacg atacctaaaa
    26581 cttctggcat cgaatatatt gcttttgagg tcttctctgc gtttggtaca gtaggtctta
    26641 cgatgggatt gacgccagac ttgactgcaa tagggaaatt gatcattata gcgcttatgt
    26701 acattggacg agttggtgta ttgactgtgg tcttctctct gatgacaaaa gcacaaagaa
    26761 cacaatcaaa atacaaatat ccagaagaaa gtgtcatgat tggataattt catcttctga
    26821 cagaaaccta gtgatttagt aaaataagaa aataaaataa tttttcttgc ttcttggata
    26881 ctttaagtgc aaaatgttcg tttatttttt gaggcactac ccgttgttga acctcgggta
    26941 gtgcctcttt tttattgaaa ggaatcaata agatttgatt cctttattta tcaataggtt
    27001 ataattcata tttttttatt ttttctttta cgatacttag tccagaagct aaaatttcaa
    27061 ggttattaga ggatagggaa attcgaacga atgcatctgg ttgcgcacgt gtagtaaagc
    27121 gtctggaatg ataaagtcgg ataccgttag caagaaagtc attctcaatt atcttagggt
    27181 caatctcagg atcaactgga agaattcgat agtaagggac acgattcgtt ggtttttcta
    27241 ctttgaaaag agaatcaaaa agctgattgg ctctttttgt aagacttaat ttagtttcta
    27301 agatcctatt tgcttcattt gaatggagga tttgagtgat gacttccaca tctaatccag
    27361 aagttttcac atttatatta aaaattgctt gttctatctt gctttggaca tgtactggaa
    27421 ctacaagata agcaactcgc aatccagaac aaataaattt tgtcattcca gctaagtaga
    27481 ttgtttggtt aggtaataaa ttttggaaag gaggaatgat cgtatgttga tagtaagttg
    27541 ttaaaaatga atggatatca tcttcaataa cccatagacc ttctttttca atcacttcgg
    27601 ctaattgctc tcgtcttttg tgagagattt gaaaccctat aggattatta caagaaggca
    27661 taagaaagac accatgaatc ttcttttttc tacattcctg aatcaatata tcaggtcgca
    27721 ttccttgctc gtcaaaatca attggaacga tctctaaatg atataactga gccaattcaa
    27781 tgaaattagc gtatgtatat cggtctgttg ctattctatc acctggagag aaaagtgcag
    27841 aaagtgtgat agccaatcca ttttgaacac cggaaacaat agatagagat tctaaggacg
    27901 gaatgactcc ttgagttttt aaccaatttg ctgcagtagt caattgaaaa tttgtgcctt
    27961 tgggatcttg gtaattaagc aaaccaacaa gatttgggtt attactagtt gattgaataa
    28021 acgggacaac gaatttatta cattcttcaa aagaactaac cagaccaaaa tcaatgatat
    28081 gttcggttac ttcattggta gagatggtga tggactcaaa gacattggga gaaataaatg
    28141 tgccacttcc aatattcgta tacagcaaac ctttctcaat tccatattta taagccttgc
    28201 caacggtagt aaaatttaaa tctaaataat cagctagctc tctttgggag ggtaactgag
    28261 tgtttttctg taatttattc tttaaaatat cttgttctaa ttgttgaatc aacgatcgat
    28321 aataaggacg cttcaatttt tgtttgtctg gtcgccaagt taacgggtaa ttgtcataag
    28381 aattaatagg cattttctcc actccttaaa attgtatacc atacaatttt accattgatt
    28441 gtattgattt tagaagttaa tcttttgtga ggagggaatt gtctatgaaa attattaatt
    28501 ttgaggaagt agtagcttct tttagttttg aagaagcgat tcaagtaatg aaaaaatgtt
    28561 tttttgatta tgaaagtggt aaaatcagcc aaaatcctcg attggtggaa attttacctg
    28621 acggaaaagg caaaaatatg tttgcgatga tgcctgctta tttgggggag aacagatatt
    28681 ttggcacaaa agtcattacc gcttttccgg ataatcatca gtatcattta ccttctcatt
    28741 taggagagat tttcttgttt gatgctcaaa acggactacc caaagcattg ataaacgcaa
    28801 atgcagtcac ctggattcga acagctgctg tatctgcgct ggcaacagat tacttagcaa
    28861 atccgcatgc agaaacactt gctttaattg gagctggtca acaagcattg tcgcatttga
    28921 ctgcgatttc taccattcgt cccatcacta cagtgtttgt ttacgatatt ttagaagaaa
    28981 gacgaaatac ttttatcgct aaagctaaaa aacagtatcc taaaataacg tttataaatg
    29041 caatatcagt agaagatgca gtacgtccag cggaaatcat ttgtacgttg acctctagta
    29101 aagaggcatt tcttcaaaaa gagtgggtaa gggaagacgc acatatcaat gctgtgggca
    29161 catttactcc tgatacaaga gagataagta catcgttgat ggaaacttct caaatctatg
    29221 tagatgatta ctcagctgcc ttaacagaaa gcggggacct gcttatagcc atggcagaag
    29281 gagatttaga ggaatcttgt attgttggta cattaggcga attagtttct gagaaaaaaa
    29341 cattttataa aggagaatcg aggccgacga ttttcgacgc agttggtcta gctattgaag
    29401 atctttgttg tgcagaatat atttataaca aattagagaa aggggcttta aaatgaactt
    29461 agattattac gtagttgatg catttgcaga tgaggtgttc aaaggaaatc cagcagcggt
    29521 ttatgtgcta gaagaatggc tgccagaagg tacgatgcaa aaaattgcca ttgaaaacaa
    29581 tttatctgaa acagcattta cagtgaagaa aaaccaagaa tttgagctta gatggtttac
    29641 gccagatcga gaaatcgatc tttgtggtca cgcgacatta gcaacagcat ttgttttatt
    29701 taactattac aaaatacctg atgaaacgat taaattttct actcaaagcg gtaatctatt
    29761 tgtgacaaga attaaagatt attattacat ggattttcct agtattatgc cgaaaaaagt
    29821 gccgatattg tctgaatatg aagaagcgat tggggctaag attaaagaag cctacttagc
    29881 acgtgattta tttttcgtat tagaagatga aaaaacggtg gctaaattgc agccagattt
    29941 tacagctatc aaaaattttg aattaggaat aggagtcatc gtgacagcag aaagtgtgca
    30001 aaaagacttt gtttctagaa ccttctttcc aaaattaaca atcaatgaag atccagtatg
    30061 tggatcagca cattctaatc ttattcctta ttgggcagaa agaatgaata aaacaaaatt
    30121 gtcagcttat caaatgtcat caaggggcgg ggatctagaa tgtgaattaa aagaagatcg
    30181 tgtgataata ggaggaaaag cagttttgtt ttctaaaggt acctcgttta tttgatttct
    30241 ttcaaatgaa ttaaaaataa attggtggag agtggcaacc ttatagctac aatggttgtt
    30301 aaaaaaagac gcaaactttt tcaatttaat gaaaagtttg cgtctcattt taattattgg
    30361 tgtttgtttt ttattcaatc aagagtaaaa tgccccatac taactatcct cggatgactt
    30421 cgattttata accatcaggg tcaatgacaa agtagtaaga aggcgcagtg ccaggtaatc
    30481 cttttaagtc agtaatctca agaccagcag ctttatgttg ctcgtgtaat ttttctagat
    30541 catcagctgc tatagcgata tgaccatagc cgtctcctag atcatacgca ccatgatcat
    30601 agttgtaagt caactctaat tcatagtcat ccccaggaag tgtcaaataa acaagtgtaa
    30661 atttgttttc tggaaaatca cggcggcggc tttcttcaaa accaaatgcc ttttgataaa
    30721 attcgattga tgcttcaaga tctttcactc gaacacatgt gtgagccatt ttcatagatt
    30781 catctctcct tttcctttta ctcaatcata acaaaaagaa gtaaggaaat agttgatttg
    30841 tgcttgttca tgagaaattt ctcatgtaaa atggatagaa aataaaactg ttaaggtgga
    30901 aactattcat ggataaacca atttttggtg aaaaaaagca atttgctgat tatcaaaccc
    30961 gttatgcagc atatattgtc atagaaagag atcagcagat tgcagtaatc gaggctccaa
    31021 atggtgcttt ttttcttcct ggcggggaaa ttgaaggaga agagacgaaa gaagaagcga
    31081 ttgtccgtga gctgatagaa gaaatgggaa ttgtggcaga aatttcttct tatctgggag
    31141 aagctgatga gtatttttac tcgaattatc gtgcaactta ttattataat ccaggatatt
    31201 tctttgttgc tgattcttgg aagcgtatag gagaaccaac tgaaaagacc aataaaattt
    31261 ggtgggtgag ccctcaagaa gcgcttgtga aattgaaacg tggtagccac cgatgggcag
    31321 tcgagagatg gatcgaaaaa aataaaaact gaaagaaagc gagtagatca gctttctctc
    31381 agttttttta tttattacag tacatatttt ttgattgctt cagctacacc gtgctcgtcg
    31441 tttgatgaag tgatgacatt tgctgcttct ttgacaagag aaacggcatt tcccattgct
    31501 actcctagac cagcatattc aatcatggat aagtcgttct cgttatctcc gatagccatg
    31561 atttcatctt gatcaatacc aaggtgctgt gcgagattcg caactgccgc acctttactt
    31621 gcttctttat ttagtacttc gaagtaatag ggcgcacttt tgaccgttgt gtatttatca
    31681 cggaaagttt gcggtaaacg cgcaattgct gcatctaaaa tttctggttc gtcgatcatc
    31741 atcattttga cgatgttcat gtctggtgtc atttcttcag gtgttcgata ttttagcggc
    31801 atcttgacta aacttgcttc gtggatcgta taagcgctga tatctcgatt aggcgtgtag
    31861 atcgctttgt catcgatcga atggagatga ctgccgacac ggcgtgccat tacttcgatc
    31921 tctaggaaat cttcatgtgt cagtccgtga cgggcgatga ttttacctga tttcgtttgt
    31981 tgtacgagtg ccccgttata agtaataacg taatcattat cttgaaaaag gttcaactcg
    32041 tttaactgtt cctggacacc tggtaaggga cggccagtgc ataaaacgat cgaaacacct
    32101 tttgcaacag cagcggcaat cgcttctttt acttcttttg ttacttctct tttctcattt
    32161 aataaagtac catcgatgtc gatagcgact agtttgatag acataatttc ctccaatata
    32221 ttttaattta tttcaatcaa tgcgccatta cggatatggc tggcaaattc ttgataagtt
    32281 tcgtcaaata aatcataatg atcacgcaag gacggatcaa ccatttcttt tggaaagaaa
    32341 aatcgctcat ctccttgttc ttgtcccgct aatgcagcaa caagctgact aacctgtgat
    32401 aattctatca aagttccgtc atttcgcaaa atctcgattt gtgtgcgatg tctgccttgt
    32461 tctgggcgat aaaaatcata aggtagatcg tagctagaat tcacagcagt gtaataaaca
    32521 ggattatatc cgaccttttc tactaacaaa gtgagttctt ggactaattt tgaatcccgg
    32581 ttgccagaaa aagtagcaga tttcagtggt ttacgattca aaaaacgttt agctaaatca
    32641 tttaaaatag gatcagcaga ttcagaccat tgagtaaagt aagtgcccaa gaccccatca
    32701 tcaagtttga ggtattcttc taaagtaaag cttccgtcaa gaaatggcag aagtaattga
    32761 gaatggagtt caaaactgct ggcatcttct tgatacaact cgtgtgcgcg atgaagcaga
    32821 tgctccaaaa ttacttccat accacgggaa acaggatgga agtacacttg cacatacatt
    32881 tgatatcggc taacgatata atcttccact gcatgcatgc cactcatgga aaaagcgatc
    32941 ccgccttcat aaggacgaat cacacgtaat attcgggtaa gatcaaatgt tccatattct
    33001 gttccagtga aataagcatc ccgaagcaaa tagtccattc gatctgcatc gatctgacta
    33061 gaaatcattt ggacaacttg tgggttgggg taagtttttt ggatcacact agctaccttt
    33121 tcaggaaaac cttcttctac tcggttaaga atctgataca cttctgtttc tggagaagtg
    33181 atgatctcta cagtgatggc ttcatgattc gtatggaaga tatgttcaaa tgtatgagag
    33241 taaggaccat gaccgacatc atgcaacagt gccgcacata aagtcaccag ccgttcctga
    33301 tcatcccagc ctccgttgcc tattttttct tttgaaaaat ttcgagaaaa gatatcgcag
    33361 attcgtcttg aaatctcata aacgcctaat gaatgagtaa aacggctatg ttctgctccg
    33421 tgaaaagtga aagaagaagt gcctaattgt ttaatgcgtc gtagacgttg gacttctttc
    33481 gaattgatca aatctaagat tacttgatgc tggacatgga tatagttatg gactggatca
    33541 cgaaaaactt tttccatagg cagcatttga tatttataag gggttgtcat aatgtagctc
    33601 ctttatgtta ttacgttgtt ccattctctg gatttggttg ttccattgct ccagctggat
    33661 ttcttttttt agccagtcgg tagaatcctg taaatcactt gcagaataca gcttttcaaa
    33721 agcttttgca aatgcttctt tgacttcttt tacaaaaagg ggttggtcaa gcagttcttc
    33781 aagtgttgcc attgtagcag gatccactgc aggatagcca tttgtaccaa agtcttcttt
    33841 taaagcagat tgataaaatc gcttcatgac ttgcccacgt ttttcctgat taccgttgac
    33901 acttaagtac atcataacag aaatcccatt tttgatccga cgctgagcga taccggcaaa
    33961 ttttttgcca tggatgctta aatcataagt tccaggacaa taagaatggg tgatttcgaa
    34021 tgcttgtatc ggtgcctttg gaaaagcgta tttagtcaat aggagcattt gctcgtaagc
    34081 gtcatctatg gttatttttt ttgtgtcact ttgggggaaa atcaaagaaa tattcaaaat
    34141 tccttcatct gatacgatcc ctaatccacc tgaatttctg atcactggag cgtaacctgc
    34201 atcttccagt gtttggatcc cgtcacttag aaaaggagtt cgaacatcct tcatacctaa
    34261 gatgactgtg cagtcatatt gccagaaatg taaaaagatt tgctggtgtt ctttagaata
    34321 ttccgttaat atatctgtta atgcaaaagg ggaaaagtcg tttttttgat aaatgtgctg
    34381 atctagtcga atcgcagggt tcattcaacc ggctcctttg tataaattca tattttctac
    34441 aatattatac tctaaaatca aagagttgtt ttggaaaaaa ggttttctgt aaaaatggac
    34501 aagttggttg acaaaacaaa agaaactagg aacaatgtag ctatgagaag taaaggagaa
    34561 tgaataatgg aattatcaca aggaacaccg atttcaatca aattgcgtac aaaagtaaaa
    34621 cagcaagggg aagtgcaaga tttttatttt gatttgaaag ggcaaatggt gacaattggc
    34681 gatacgctgt acatacgtta taaagagatt caggaggaga cgggcgatga gattcctgta
    34741 acgatcaaat tagttccaga tggtcatgta cagctgatcc gtgcaggaga aatgcggatg
    34801 cgtttgaaat ttggctataa agaacggtta gaaacatcct atcgaacacc atacggtatg
    34861 atccagattg ctacttttac gaaagaactg catgttagtt tgaaagaccg tcccacagca
    34921 gggaaggttc gaatcgatta tgatctgttc atgggaccgg aaaaaatcgg cgaatattat
    34981 ttgaccttgg attttacagc gtaaatataa aagaaaagtt tgattttttg tgtctgattt
    35041 tgtatgataa ggagtagact gtgaaaggac gtgtcctgat tggaaattag tgtatttgaa
    35101 ggtgcaaaca aaagcgaatt atcaatgatc gaagtagcac acgcgatctt agaacaacgt
    35161 ggcgatgtca tggatttttc tgatttagct aaccagatcc aaaactatct tgggaaatca
    35221 gacagtgaaa ttcgtgattc actcgcacaa ttttatactg acttgaatat tgacggcagc
    35281 tttatctcat taggggataa tcgttggggc ttgcgttcat ggtatgctat cgattcgatc
    35341 gatgaagaag taaaccatgg aatggacgaa gaggatgaag atactccacg tcgccgtaaa
    35401 cgcaagaaag tcaacgcctt tatcaatgat gatgaagacg cgatcgatta caatgacgat
    35461 gacccagaag atacagatct gacagaagaa gatgacgatg atctgtttga tgacgatgat
    35521 gatgaagatg aagaaatcgc tgcttataat tctgacttgc aagaaatcgg agcagatgac
    35581 ccgacagatg atgaagaaga cttgccagga aatatcgaag aagatttaag tatcatagac
    35641 gaagacgatg atgatgaaga ttatgaagag gaagaatttt ctgactctga aaaagaataa
    35701 acattatttt cttacgagag gttgtgacag agacgatcag cctatcagaa taaggaggtg
    35761 tgacacgatt tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa
    35821 aaataaaaaa attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg
    35881 agtggagaat gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc
    35941 tgctgaatgg actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg
    36001 tacatgataa agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact
    36061 attttgaaaa agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc
    36121 gcacggcatt agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc
    36181 aagatatccc ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag
    36241 ataaaataga cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt
    36301 tgaaagaaat tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc
    36361 gctcattgaa tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc
    36421 caaaaagaaa atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg
    36481 cttaaagagg agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag
    36541 cggagaaaga gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca
    36601 agtagccttc tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga
    36661 tcagtcattg aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt
    36721 caggtaccga agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat
    36781 caaaaatact ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat
    36841 tttgaaacgg atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg
    36901 tttctcgtga ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga
    36961 tacgacacaa tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat
    37021 gacacctatc tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag
    37081 gaagatccat ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa
    37141 ccaacacttg ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg
    37201 actttttctc aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct
    37261 aagtccgtga caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt
    37321 agttgcgact agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa
    37381 gagaaagaag agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg
    37441 tgcttttgtt tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa
    37501 cacactcttc ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa
    37561 gccaatcaac tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac
    37621 aaagtcagtc atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca
    37681 aaagcgtcgc aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc
    37741 gaatcttaaa agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc
    37801 agcatcctga aatcgtgaag cagacagctg aagcagggca cgagattggc aatcacacat
    37861 acgatcatca gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag
    37921 cagacactga gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg
    37981 gttctgtcac gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg
    38041 attcagaaga ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag
    38101 tatacgatgg ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc
    38161 ctcaagtgat cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat
    38221 tagggcatcc aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt
    38281 aagtgagaca agtatcttta ataaataaca cattttaata agtagttgaa cacataacta
    38341 ccttacttgc aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt
    38401 ttctttggcg gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa
    38461 aatagcagta gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac
    38521 gtgatatcca ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa
    38581 attacaaggg gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag
    38641 cagacacaga aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat
    38701 aacagtgagg agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa
    38761 gttgttgagc cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga
    38821 gaagagacaa attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg
    38881 atcgaacaag tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt
    38941 gtagaataca tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag
    39001 attggagcta cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca
    39061 gctggagtag gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat
    39121 tatgcgccgg aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga
    39181 aaatggaaag aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc
    39241 gactatttac aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg
    39301 tacattattc ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca
    39361 aaccgaaaaa atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa
    39421 caagcagata aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg
    39481 aagaaaacga tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc
    39541 cggcaaaaag ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa
    39601 gttgcaactg agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat
    39661 ttggaaacag acttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg
    39721 aatagtacag aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa
    39781 ttagtcgatc aagaagacaa aaacaagccg ttgtctgacc agaaattggt tgaattgctg
    39841 aaaaaagatg agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga
    39901 atcccgagtt catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa
    39961 gtatttcact ttttctagtc tttttagttg aaaaacgaga gcaaaactgt tagacttaca
    40021 gtcgtgaggt tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt
    40081 atcgtatatg ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc
    40141 tatgctggat gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca
    40201 agaacgctat aaaattttac gaaatatcta ttggatgcag ccgattggac gtcgaagttt
    40261 atcggaaagt atggggatca cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa
    40321 tctgaatttg atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt
    40381 ttatcaaagc cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca
    40441 gttggcagaa tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca
    40501 gaccaaagtg gctgatgcat tcggcgaagc gctgagcgaa gccctagacc gactacttcc
    40561 agaaggcgag aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca
    40621 attgtctaat ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat
    40681 tggtgaagcg ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa
    40741 cggtaaccac cgtgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt
    40801 actcaatgag ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat
    40861 tcatagtatt gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt
    40921 agtcatgctg aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa
    40981 aggtgaagtc gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt
    41041 cccgatcatc atggcaatcg cgggcggaaa atcaaaagct aaagcaattc gagcttatat
    41101 gaaaaatgca ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt
    41161 aaaaggggta accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa
    41221 tgacagttaa agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta
    41281 tccaagatgt agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt
    41341 tggcacactt gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc
    41401 atgaaggttc attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag
    41461 aagaattacc atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca
    41521 cttctaaatc agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg
    41581 ctcctggcgg aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg
    41641 gtaaagaaac agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta
    41701 aaacattgaa cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca
    41761 caggtgacca aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg
    41821 ctgctgctgc aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa
    41881 ttcctgaatt gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt
    41941 cattaacaga attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag
    42001 caatgaaaga agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcttcag
    42061 atatcgttgg tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag
    42121 ttggcgacaa acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg
    42181 cacaattagt acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt
    42241 gtaaattata gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt
    42301 tgataataag tttattcagg aggaaataaa atggctaaga aaacagtgaa agatatcgat
    42361 ttaaaagaca aaaaagttct tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg
    42421 atcactgacg acactcgtat caaagcagca ttgccaacaa tcaactacgt tctagaacaa
    42481 ggcggaaaag caatcctttt ctctcacctt ggacgtgtaa aaacagaaga agataaagaa
    42541 ggaaaatctt tagctccagt tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca
    42601 ttcgtacctg aaactcgcgg agaacaatta gaagaagcaa tccgcaacat gaatgacggc
    42661 gacgttgtcg tattcgaaaa tacacgattt gaagatattg acggtaaaaa agaaagcaaa
    42721 aatgatacag aactaggtaa atactgggct tcattaggcg atgtatttgt taacgatgct
    42781 ttcggtacag ctcaccgtgc acatgcttct aacgtaggta tcgcttcaac tggtatccca
    42841 acagttgctg gattcttgat ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa
    42901 ccaaaacgac caatgattgc aatccttggt ggcgcaaaag tttctgataa aatcggcgta
    42961 atcgaaaact tgattcctaa agcagataaa atcttgatcg gtggcggaat gacttacaca
    43021 ttctacgaag caaaaggaat caagatcggt aactctctag tagaagctga taaagtggaa
    43081 ctagcaaaag aattaatcga aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc
    43141 tgtgcaccag aattttcaaa tgatgttgaa acacaagtaa tcgaaggaga cattcctgat
    43201 ggtttgatgg ctttagacat cggcccagca tcagtaaaat tatttgctga tacattacaa
    43261 ggtgcaaaaa cagttgtttg gaacggacct atgggtgtat ttgaaatgag taactttgct
    43321 aaaggtacaa tcggtgtttg tgaagcaatc gctaacttag aaggcgcaac aacaatcatc
    43381 ggtggcggag actcagctgc agcagcagaa caattaggat ttgctgacaa attcacacac
    43441 atctcaacag gtggtggcgc aagcttggaa ttgcttgaag gtaaagaatt accaggacta
    43501 gcagcaatca acgataaata aacgaccaag tttgctcaat tctgacgagt aatcgatcaa
    43561 aaggtgaagg gagttttcat gactccctag ccatttttca ataaattaag gagtgtgttc
    43621 ctcaatgcgt aaacctatta tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc
    43681 aaaagaattt gctgaagcag taaaaaacaa aatcccttca aatgacaaag ttgattcagt
    43741 catcggatct cctgcattat tcttacaaga attaatgaca tcagctgaag gtacagaact
    43801 aaaaatctca gcacaaaact gctactggga aaactcaggt gcgttcactg gtgaaacttc
    43861 accagcagct cttgctgatc taggtgttca ttatgtgatc atcggacatt cagaacgtcg
    43921 tgaatatttc catgaaacag acgaagacat caataaaaaa gcaaaagcta tctttgcaaa
    43981 tggtatgaca ccaatcctat gctgcggcga atctttagaa acctatgaag caggcaaaac
    44041 tgctgaatgg atcgaaggac aaattacagc tggtttagct gatctttcag ctgaacaagt
    44101 gtcaaacatg gttattgctt atgaaccaat ctgggcaatc ggtactggta aatcagctga
    44161 cgcacaaatc gctgacgaaa tttgtggagt tgtacgtcaa acagttgaaa aactttatgg
    44221 aaaagaagta tcagaagcag ttcgtatcca atacggcggt tctgtaaaac cagaaaatat
    44281 tgctgaatac atggcaaaag aaaatgttga cggtgcttta gtcggtggcg caagcttgca
    44341 agcagattca tttttgtcat tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa
    44401 atgcaagaaa tgtttgcact aatgagcaaa attaactaaa atcgtatatg agggaaatcc
    44461 cttaggaaaa aacaaactca aaggagagac aaaacatgtc aattattact gatgtttacg
    44521 ctcgcgagat cttagactca cgtggtaacc caacaatcga agtagaagta tacacagaaa
    44581 gcggagcttt tggccgcgga atggttcctt caggtgcttc aactggtgaa tacgaagcag
    44641 ttgaattacg tgacggagac aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg
    44701 acaacgtaaa caacatcatt gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg
    44761 ctatcgacaa agctatgatc gctttagacg gaactcctaa caaaggtaaa ttaggtgcga
    44821 acgctatttt aggtgtttct atcgctgttg cacgtgcagc tgctgactac ctagaagtac
    44881 ctttatacca ctacctaggc ggattcaaca caaaagtatt gccaacacca atgatgaaca
    44941 tcatcaacgg cggatcacat gctgataact caatcgactt ccaagaattc atgatcatgc
    45001 ctgttggcgc tccaacattc aaagaagctc tacgtatggg tgctgaagta ttccacgcat
    45061 tagcttcaat cttgaaagct cgcggattag caacttctgt tggtgacgaa ggtggatttg
    45121 ctccaaacct tggttcaaac gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag
    45181 ctggctatgt acctggtaaa gacgttgttc ttgctatgga tgctgcttct tcagaattct
    45241 acgacaaaga aaaaggtgtt tacgtactag ctgactcagg cgaaggcgaa aaaacaactg
    45301 acgaaatgat caaattctac gaagaattag tttctaaata tccaatcatc tcaatcgaag
    45361 atggattaga cgaaaacgac tgggacggat tcaagaaatt aactgacgta ttaggcgaca
    45421 aagttcaatt agttggtgac gacttgttcg taacaaacac tcaaaaatta tctgaaggta
    45481 tcgaaaaagg aattgctaac tcaatcctta tcaaagtgaa ccaaatcggt acattaacag
    45541 aaactttcga agctatcgaa atggctaaag aagctggcta cacagcagtt gtatctcacc
    45601 gttctggtga aacagaagat tcaacaatct ctgatatcgc tgttgcaaca aatgctggcc
    45661 aaatcaaaac tggttctcta tcacgtactg accgtattgc taaatacaac caattactac
    45721 gtatcgaaga ccaacttggt gaagttgcag aatacaaagg tttgaaatct ttctacaact
    45781 taaaaaacaa ataattcttt tctaatgaag atacatgatc gactattgaa agatagtcga
    45841 gtatgatcac ttagttaagt aatcgaactc tctacttgct gaaatatcag caggtagaga
    45901 gt
//
LOCUS       DAZKNH010000012        45477 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000012 DAZKNH010000000
VERSION     DAZKNH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.012"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..1728)
                     /locus_tag="ACG8MG_000621"
                     /pseudo
     CDS             complement(93..1728)
                     /locus_tag="ACG8MG_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2051..2530)
                     /gene="rlmH"
                     /locus_tag="ACG8MG_000622"
     CDS             complement(2051..2530)
                     /gene="rlmH"
                     /locus_tag="ACG8MG_000622"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFQ0374690.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2958..4247
                     /locus_tag="ACG8MG_000623"
     CDS             2958..4247
                     /locus_tag="ACG8MG_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFQ0374691.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4286..4891)
                     /gene="ytpR"
                     /locus_tag="ACG8MG_000624"
     CDS             complement(4286..4891)
                     /gene="ytpR"
                     /locus_tag="ACG8MG_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFQ0374692.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5096..5560)
                     /locus_tag="ACG8MG_000625"
     CDS             complement(5096..5560)
                     /locus_tag="ACG8MG_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0374693.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5582..5902)
                     /locus_tag="ACG8MG_000626"
     CDS             complement(5582..5902)
                     /locus_tag="ACG8MG_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0374694.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(6046..7125)
                     /gene="pepA"
                     /locus_tag="ACG8MG_000627"
     CDS             complement(6046..7125)
                     /gene="pepA"
                     /locus_tag="ACG8MG_000627"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFQ0374695.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7325..7675
                     /locus_tag="ACG8MG_000628"
     CDS             7325..7675
                     /locus_tag="ACG8MG_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0374696.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7898..8845
                     /locus_tag="ACG8MG_000629"
     CDS             7898..8845
                     /locus_tag="ACG8MG_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0374697.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8902..10320)
                     /gene="pepV"
                     /locus_tag="ACG8MG_000630"
     CDS             complement(8902..10320)
                     /gene="pepV"
                     /locus_tag="ACG8MG_000630"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFQ0374698.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10469..11314
                     /locus_tag="ACG8MG_000631"
     CDS             10469..11314
                     /locus_tag="ACG8MG_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFQ0374699.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11318..11758
                     /locus_tag="ACG8MG_000632"
     CDS             11318..11758
                     /locus_tag="ACG8MG_000632"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFQ0374700.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11808..12002
                     /locus_tag="ACG8MG_000633"
     CDS             11808..12002
                     /locus_tag="ACG8MG_000633"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374701.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12138..12824
                     /locus_tag="ACG8MG_000634"
     CDS             12138..12824
                     /locus_tag="ACG8MG_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0374702.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13232..13921)
                     /locus_tag="ACG8MG_000635"
     CDS             complement(13232..13921)
                     /locus_tag="ACG8MG_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374703.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13896..16025)
                     /locus_tag="ACG8MG_000636"
     CDS             complement(13896..16025)
                     /locus_tag="ACG8MG_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFQ0374704.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(16073..17410)
                     /locus_tag="ACG8MG_000637"
     CDS             complement(16073..17410)
                     /locus_tag="ACG8MG_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFQ0374705.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17662..18021)
                     /gene="rplT"
                     /locus_tag="ACG8MG_000638"
     CDS             complement(17662..18021)
                     /gene="rplT"
                     /locus_tag="ACG8MG_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFQ0374706.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(18074..18274)
                     /gene="rpmI"
                     /locus_tag="ACG8MG_000639"
     CDS             complement(18074..18274)
                     /gene="rpmI"
                     /locus_tag="ACG8MG_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFQ0374707.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18346..18870)
                     /gene="infC"
                     /locus_tag="ACG8MG_000640"
     CDS             complement(18346..18870)
                     /gene="infC"
                     /locus_tag="ACG8MG_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFQ0374708.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(18914..19039)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19190..19822
                     /locus_tag="ACG8MG_000641"
     CDS             19190..19822
                     /locus_tag="ACG8MG_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0374709.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(19824..20729)
                     /locus_tag="ACG8MG_000642"
     CDS             complement(19824..20729)
                     /locus_tag="ACG8MG_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0374710.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(20814..21473)
                     /locus_tag="ACG8MG_000643"
     CDS             complement(20814..21473)
                     /locus_tag="ACG8MG_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0374711.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            21789..22733
                     /gene="mvk"
                     /locus_tag="ACG8MG_000644"
     CDS             21789..22733
                     /gene="mvk"
                     /locus_tag="ACG8MG_000644"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFQ0374712.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22726..23703
                     /gene="mvaD"
                     /locus_tag="ACG8MG_000645"
     CDS             22726..23703
                     /gene="mvaD"
                     /locus_tag="ACG8MG_000645"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFQ0374713.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23716..24801
                     /locus_tag="ACG8MG_000646"
     CDS             23716..24801
                     /locus_tag="ACG8MG_000646"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFQ0374714.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            24798..25862
                     /gene="fni"
                     /locus_tag="ACG8MG_000647"
     CDS             24798..25862
                     /gene="fni"
                     /locus_tag="ACG8MG_000647"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFQ0374715.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26111..26914)
                     /locus_tag="ACG8MG_000648"
     CDS             complement(26111..26914)
                     /locus_tag="ACG8MG_000648"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFQ0374716.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(26934..27776)
                     /gene="kduI"
                     /locus_tag="ACG8MG_000649"
     CDS             complement(26934..27776)
                     /gene="kduI"
                     /locus_tag="ACG8MG_000649"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFQ0374717.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(27792..28799)
                     /locus_tag="ACG8MG_000650"
     CDS             complement(27792..28799)
                     /locus_tag="ACG8MG_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFQ0374718.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(28806..29450)
                     /locus_tag="ACG8MG_000651"
     CDS             complement(28806..29450)
                     /locus_tag="ACG8MG_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFQ0374719.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29550..30308)
                     /locus_tag="ACG8MG_000652"
     CDS             complement(29550..30308)
                     /locus_tag="ACG8MG_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFQ0374720.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30435..31586)
                     /gene="fucO"
                     /locus_tag="ACG8MG_000653"
     CDS             complement(30435..31586)
                     /gene="fucO"
                     /locus_tag="ACG8MG_000653"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFQ0374721.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31668..31979)
                     /gene="rhaM"
                     /locus_tag="ACG8MG_000654"
     CDS             complement(31668..31979)
                     /gene="rhaM"
                     /locus_tag="ACG8MG_000654"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFQ0374722.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(31981..32826)
                     /gene="rhaD"
                     /locus_tag="ACG8MG_000655"
     CDS             complement(31981..32826)
                     /gene="rhaD"
                     /locus_tag="ACG8MG_000655"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFQ0374723.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(32840..34096)
                     /gene="rhaA"
                     /locus_tag="ACG8MG_000656"
     CDS             complement(32840..34096)
                     /gene="rhaA"
                     /locus_tag="ACG8MG_000656"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFQ0374724.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(34093..35553)
                     /gene="rhaB"
                     /locus_tag="ACG8MG_000657"
     CDS             complement(34093..35553)
                     /gene="rhaB"
                     /locus_tag="ACG8MG_000657"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFQ0374725.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35706..36575
                     /locus_tag="ACG8MG_000658"
     CDS             35706..36575
                     /locus_tag="ACG8MG_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0374726.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36701..37600)
                     /locus_tag="ACG8MG_000659"
     CDS             complement(36701..37600)
                     /locus_tag="ACG8MG_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0374727.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(37765..38589)
                     /locus_tag="ACG8MG_000660"
     CDS             complement(37765..38589)
                     /locus_tag="ACG8MG_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0374728.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38600..39895)
                     /locus_tag="ACG8MG_000661"
     CDS             complement(38600..39895)
                     /locus_tag="ACG8MG_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFQ0374729.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(39911..40390)
                     /locus_tag="ACG8MG_000662"
     CDS             complement(39911..40390)
                     /locus_tag="ACG8MG_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFQ0374730.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40396..41424)
                     /locus_tag="ACG8MG_000663"
     CDS             complement(40396..41424)
                     /locus_tag="ACG8MG_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFQ0374731.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41441..42547)
                     /locus_tag="ACG8MG_000664"
     CDS             complement(41441..42547)
                     /locus_tag="ACG8MG_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0374732.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(42776..45373)
                     /gene="adhE"
                     /locus_tag="ACG8MG_000665"
                     /gene_synonym="adhC"
     CDS             complement(42776..45373)
                     /gene="adhE"
                     /locus_tag="ACG8MG_000665"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFQ0374733.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT        13163 a         9671 c         8251 g        14392 t
ORIGIN      
        1 attaagcttc attattataa tataaagttt aaatttggtt tattacttac tttttcttat
       61 tctctagtaa gctaatattg aaaaaaatgt aatcagatca ttatttttat atgttgtaac
      121 ttcctgataa gaaaactaat taaaagaaag aggtaactac ataaactaat taaaaaagct
      181 aacttcatag ttggaggata aatggaagaa acttcaatga ttgtttctcc ttgtgttcct
      241 tcaatcgaca ctagtcctat tatcaatctc tcttttttgt ccatttactt ttgcgactgt
      301 tccccaataa tcaaatacag gaagcgttat tttttgtcct ttagattcat gtactgtata
      361 gactattttc gaatccatca cttgataagg catagtatgc cattccttgc caattcttac
      421 ttcatgattc tgcaaatgtt ctttgtattg taatgcttcc gtgggtgcat agtcataaag
      481 tcctccggga aggagtgtcg tgtaacgaat aagattttct tgattcatta ccaagtgatc
      541 tctctgagtg acaactttat gttcatagtt tgcttttgtt gcagcagata cagccaacag
      601 taataaaaca attgccgtta tattaactac ttttctttgc ttagctgatg tgctcttcat
      661 gaaaacgaac gataaaatca acgagccaaa cagtacttga aagcctaaga ttcgccaagg
      721 aaattgaatc atttgaatgg gtgtattttg taataaagac catggaaaaa atttggtgga
      781 taagagccaa gagactaccg cactaaggta aatcactttt tctttgcgat taaatccttt
      841 aaaaaagaag agtggacaga tcaaagaaat aataccaatg attcctaccg taaaggttgt
      901 caggtcatta ttcatcgcac caatcaaact atccgagaca tttaatgctt ggtgttgtaa
      961 ggtcgtttta tctggctcat taatcgattg aaccatgatt tgtcggaaca tcggatacca
     1021 aaagtatgct gttaaaagaa tcgtcattcc tactgccttt gacaactcca caatcaaccg
     1081 gatgctttct ttttttcttg tttcacttac tacaaagccg cctaagacta gtaagccaac
     1141 ggctattaca ctcataaaag tggaaagaac atgtgtataa atgagtaagc tcatactgac
     1201 tgctaatgga attgcacatg gctttttctc ataaatcagt gcgtaagcat aatacataac
     1261 tatcggtata accgtaagtg caatcgcttc agcaatagca gaacgatgat aaatatcaat
     1321 cgtacggtac ccataaaatg tatataaaca gctaaatata aaagccgcat cgattctttt
     1381 taaaaatttt agtccataat gaaacatcag catgatagta caaacattca atagccaaac
     1441 atataagata taactaacga ttaaattatt acttatccaa taaaaaatga ctgccggaaa
     1501 aaaggtcaag tagggataaa aataattcac tccgctccca taatgattaa acgtcgtaaa
     1561 attgattgga ccagaaaaaa ttgaagataa ccctttgact ctagcaaaat ggaaggcaat
     1621 atcccccgat gtagaaatag tatgaacatt tctaaaataa ttgattgaat aaagtccagc
     1681 aataattgct aggagaatgg acaatattat ttttttcttt acttgcatta cttaattata
     1741 tactcctttc atggattgct ttatctagta taacttaaat tttaaataaa tcgttattta
     1801 tttttactct cacttaaaaa agcactaaaa ttataagaga gggaggtaat ggtctgaaca
     1861 ccaagaattg ttccaacatc gaattgctta actaatgagt tcaaagctta attatgtggc
     1921 tcttatattt accgagcgtg gattgcgatc tctttagcga aacaaacatc tttcataaaa
     1981 aaaacagcca tatccgcttt cttggatatg actatttact actactttac tatctttcat
     2041 tcttcttgtc tcacttatga taaggcgccc ccgcattgat ccggaaggca cggtagattt
     2101 gctcgacaag gatcagacgc atcaattgat gtggatacgt catttttcca aaagagatct
     2161 gagcattgct tcttttcatg acggcttcgc tgagtcctaa agagccgccg ataacaaaaa
     2221 caaggtggct ttttccttgg atacctaatt ggtcgatttg tttagcaaaa gattcacttg
     2281 taggattttt cccttcaatt gctaaggcat agacgtattc ttgttctttg attttcgcta
     2341 agatccgttc cccttctttt tcttttactt gtctcatttg cgcttcgctt aaattctccg
     2401 gggctttttc gtcaggaact tcgatcaatt cgatttttgc ataggcattc agccgtttga
     2461 catactcatt gataccttgg atcaggtatt tttcttttaa ttttcctact gagataattt
     2521 tgatattcat gttttctctc ctttggtctt catgctcact ttgtttatta taatcaattt
     2581 tttcgtctat atgtttattt tggctatttt tgttaaaaag atttccccaa cagttatgca
     2641 caacatccac aggtttatcc acagaaaaca gtgttatttt accttatttc atcacattca
     2701 ttaggcaaac atacgtttta taagaattta tccactttat acacaggctg tggatatgtt
     2761 ttatccctta tttttccaat cctatccgcg tagttttaca cagtatctgt ggaaaaagaa
     2821 aatcaaatat tttcatctga aaacaatgat ttttcctaaa gtttttcata aaaagttcac
     2881 agtctcaaag tagactaaag tcaacaaata agactatact aacagtagat aaatgaatgt
     2941 aattggagga ggaacacatg gatagaaaaa atgtgacacc aaagatgaaa aaaagtaaaa
     3001 ataatagcct ctggcgtaag ttaggtctgg ggcttgttgg agggatcgtt ggaggactac
     3061 tgacagctgg cattttttat gcagcgatgg gcactggcaa caatagttct actgctacaa
     3121 gcggcaatca gaattctgcc ggtgaaacag ttgtggaaaa tgtcaaagtc aatgtagatt
     3181 ctgatatcac caaagcggta gataaagttc aagacgctgt cgtttctgtt atcaacttac
     3241 aaagtcaaaa ccaatcaagc ggttttggcg gactattcgg gcagcaagaa gaaagtagca
     3301 gctcagatga tagtaatttg gaagcttata gtgaaggaag cggcgtgatc tacaaaaaag
     3361 acgggaatac tgcttatgta gtcacgaata atcacgtagt agatggccag caaggtcttg
     3421 aagtcttgat gaaagacggt acgaaggtaa aagctgaatt agttggaaca gatgcttatt
     3481 cagatttagc ggtgttgaag atcaattcag acaaagtcga aacagttgcc tcttttggtg
     3541 actcaagtgc tctgaaggtt ggtgagcctg cgattgcgat tggttcccca ctaggatctg
     3601 aatatgcaaa ttctgtcact tcaggaatca tttcttcctt gaaccgacaa gtgacaagta
     3661 cgaatgaatc gaacgaaaca gtcaatatca atgcgatcca aacagatgca gcaatcaacc
     3721 ctggaaattc tggtggtcct ttagttaata tcgaaggaca agttatcggt atcaactcaa
     3781 gtaagatcgc aagtacttct gaatctactt ctaatgtaag cgtagaagga atgggatttg
     3841 ctattccaag taacgatgta gtgaatatca tcaatcaact ggaaaaagac ggaaaagtga
     3901 cacgtccagc tctaggtatc acaatggtcg acttatctgc cgtttctact cagcaacaag
     3961 aacaaatctt gaagattcct tcatctgtaa ctaacggtgt catcgtgaca aatgtccaaa
     4021 cagctacacc ggctgaaaaa gctggattga aacaatacga cgtcattact aagatcgatg
     4081 acaaagaagt ttcttctggt gtagaacttc aatctgcttt gtataagaaa aaagtgggag
     4141 attctgtcaa aatcacttac tatgaaggct cgaaagaaaa aacaactact gttgaattga
     4201 cgatcgatca atcggcatta aaacaaaatc aaaatagtga aaattaataa aaaagaggtt
     4261 gtgacaaaga cttgtcacaa cctctttatt ttggaaatgc ttctccgact acggcatcct
     4321 caggcaatac taagattcct ttttttgctg gagcatttgg caaatgcaat tcacgagcgg
     4381 aacagatcat tccatagcta tctacccctc gcaaagttcc tggccagatc atcattccat
     4441 ctggcatcat agctcctggt tttgcaacga caactttttg tccagcttcg atattgggtg
     4501 caccgcaaac gatttgcaat actgtaccat tgtctacttc tgtctcagta atcgataaat
     4561 ggtcagaatc aggatgtggg acgcatgttt ttacgtaccc gacaacgatc ttcggttcat
     4621 gatcagcttc aagctgttct tcgaatcctg cttctttgat caatcgattc aatttcgcta
     4681 attcctcatc tgttaagctt acttggccgt tcccttcgat tgttagatga tcggagatat
     4741 ggaaaaagtt ccaagcaaca acttgacctt gttcatcttt gatccgagca acattccatt
     4801 tgcgttcaac agtgttttct gttcctttat cgtcggctat aataaccatc aaggtgtctc
     4861 cgacgtgtgc tttgttataa gcaaaaatca tgttctttcc tccattatcc tttgctgaga
     4921 tttatcttaa caaaaaattg cattcactgc atgaaatttt tttcatgctt tatttccgaa
     4981 gaagcctctt ctggaacacc atttattcgt aacaagggac tgtgacaaga cttttgtcac
     5041 agtcccttta ctgttttagt taattgatta aaaaggtcgt acaagttcca cttgcttaat
     5101 tacttggctg ttcttcttcg tcagaaacga tcaagacatc acaaggtgaa tgacgtatca
     5161 cgtagctggc tacacttcct gtcataaagc gttcgactgc atttagacct gactgcccaa
     5221 ccatgattag atcgatctca tatttttttg gcaatttgac tgccatggat tcacgaacag
     5281 aaccatatgc aataattcct tctatatttg tgtaatcaac agatttccca tattctttac
     5341 attcttcgat catttccttg gctgtttcag tttgctgatc gactagattt tgatcaatag
     5401 gtgcatagcc cataaagtta taaaattgct gatcgattac gtttgctaca tatactttcc
     5461 cattattacg gcgagccact tctaccgctt ttttgaaagc agcaagtgct tgttcactac
     5521 cgtcgatacc tactaaaata ttcttatatt cttgattcat cttgacccct cctaacagat
     5581 gtcagccttt tgttacactt tctaggaacg attcgatttc ctgttttgtt ttacgatctt
     5641 tattcactag acgacctaat tcttttccgt cttggatcac gacaaaactt gggataccaa
     5701 aaatgttcca ttcagcggct acatctatgt acttgtcacg gtctacttca ataaattgcc
     5761 aatcagaaaa gtcattttca atttcaggca tttgcggttt gataaaacgg cagtctccgc
     5821 accagccggc agtaaagaaa aatacactct ttccagtttc tacgtagcct gctaattctt
     5881 ctaaactatt tgggataatc attctttgtc ctctactttc tgtttatgta tttttcaatt
     5941 aaattataca cctttctttc gaactaacaa acattgatcc tctttttata aaaaaaatct
     6001 ggcaaaaagc aatcttttgc cagattttat agtttcacta tcgcattatt ttccgtatac
     6061 gatcgtattt actgttgatt tatccaaacc tttcagtgtt tgaatcaaca tttctcgtgc
     6121 agcttcaaaa tcgcgaatac tgaacatcgt ttgatgcgta tggatataac gtcccactac
     6181 gccaatcact gtacttggga ttccttcgtt ttgtgtatga gcagcacctg catctgtacc
     6241 gccttttgaa acaaagtatt gataagggat attgtttgtt tcagcgatat ccagcaagta
     6301 ctcgcgcaaa cgaggaagca tgatcaaacc tggatcttgg atacgcatca atgtaccttc
     6361 tcctaaatgt ccgaatgtgc catttttcgt cactgtatca tctgctgcag agcagtctac
     6421 tgcaaagaac agatctggct tgaatttatt gacagaagct ttagaaccac gcaagcctac
     6481 ttcttcctgg acattcgcgc ctgcaatcaa tgtatgacct aatgtttcat tttgcaacgc
     6541 ttctaaagct tctaatacca ttgtacagcc atagcggttg tcccatgatt tactgataat
     6601 attctttccg ttggctgttt tgatcgtttc agcgtaagga acgattgtgt ctcctggaag
     6661 gacaccgtaa ctcattgcct cttctcttga ttcaaaccca gcatcgaaca aaatatcggt
     6721 tacttctact gatttttgtc cgcttgttcc tcgcaataaa tgtgggggaa cggaagaaga
     6781 aatacatgga taattcttac cagtagaggt tttaagtgta aaacgctgag cagaaacaac
     6841 gtaaggattc cagccgccta gaggtacgac tttaaaaagc ccgttttctt ggatttgtgt
     6901 cagcatgaat ccgacttcat ccatatgtgc tgcgaccatt acacggggtg catccgcttc
     6961 ttcatgatgg cgcagaccaa aaatgccgcc caaaccatct aactgtaact catctaccag
     7021 cggttccata tgactttcca tataagcgcg aacatcttgt tcaaaaccgc ttgttccttg
     7081 taattctgtt aactctttga ttcgttgaaa tgttttttct tccatatgct attcgtctaa
     7141 atttcagact aaactcctct cagacttata ttgatctgcg gataaaatat gattttttct
     7201 gcagaccgat cgtatttatt atagcttatt ttttctggta gcggttattt atttcaaaga
     7261 aaatatgaga gtgtggtaaa ataagcacaa ggagtaacaa gtgagaagaa ggaggtatgt
     7321 ccacatgtat gaagaaagcg aactaagcta ttttaaaggc gggttagcag ttggaatcgg
     7381 tctaggatta ttgagcgggg tagcttcaac gatttggtat catcgcaaaa aagtattgtc
     7441 agcagaccaa gtacttgaaa atatcaagtc atcttttcta aaagaaggtg caatcgaagg
     7501 ttcttggatc tcttttgaaa aggaacctat taggaaattc gccgttcggt cgagtgctta
     7561 tcggggaggt ctttctcgaa tagaagatgg cgaaactgtt tattatgaat ttttagcaga
     7621 tgctcataca ggaaccgtat tagaaatttc aagaaaaaaa ggtgcaacag attaatacgt
     7681 caaaaaagtg agagaacgaa tacgcaccca ctattcaaaa gagaggtatc atgtccttct
     7741 tttgtagtgg gtggtttttt agatagagct ttccaaacat atattccagt attttctttt
     7801 taaataaatg atatttagcg ggaaaaacta gcatttcctt tgattatgag gtatttttaa
     7861 tttagctttt gttttctata gttaaggaga gtgaaaaatg gaacaacaag aaaaaattgc
     7921 tgttctgatt ccttgttaca atgaggaagc aacaatttca acagtcattg cagactttaa
     7981 acgagaatta ccagaagcag atatttatgt ctatgataat aattcgaccg atcgaacata
     8041 cgaattggcc gttcaaggtg gagcaatcgt aaaaaaagaa cctcgtcaag gaaaaggaaa
     8101 tgtgatccgc caaatgttct ttgatatcga tgccgactat tatttaatgg tagatggcga
     8161 tgatacttat cctgcagaag ctgtacatgg tttgctcgag aaattacgtt ctggtgaagc
     8221 agatatggtc attggtgatc gcctatcaaa tggaacctac tttgatgaga ataagagacc
     8281 tttccacgat tttggaaata accttgttag aaatacaatt actagaatgt ataaaacaaa
     8341 aattcttgat gtcatgaccg gctatcgagg attcaatcgt atctttgtca aaagttttcc
     8401 tattatgagt tctggttttc aaatcgaaac ggaattgacg atccatgctt tagataaaaa
     8461 attcaagttc gttgaaatcc caatcgatta tcgtgatcgt cctgaaggca gcgaatcaaa
     8521 attgaataca ttctctgacg gattcaaagt gatcatgatg attattaaaa tgtttaaaga
     8581 ttataagcct ttgcttttct ttgggatttg gaccatcttc ttctttttat ttggattggt
     8641 cacggggatt cctgtgattc gcgaatatat gctgacaagc tttattacac gcatcccttc
     8701 tgcgatttta agtactggat tgatgacttt agcacttctt tcattagtaa cagggttgat
     8761 tttggataca gtcgtcacga acgctaagaa agaatatgaa ttgaatctat atcatgttta
     8821 tcatgaatac cacagtagaa agtaaaaaaa ggatcaagac tgcaaaaata atttgcaatt
     8881 cttgatcctc tttttaatta attatttcgc caaacgatag atggcatctg catagattgc
     8941 tgttgctctg aacaagtcat caagactcat aaattcgttt gcttgatgca ttgtatcagt
     9001 atatcctggg aacatcgctc cataagcaac gccacgtttc aacaaacgtc cgtaagttcc
     9061 gccaccgatg atttgttcgt agcctttttc tccagtgtgg tcttcataga cttgcaataa
     9121 tgtagccacc aatggatcat ccattggtac gtaatgtggt tccattacgc gagcacctcg
     9181 agtaactgtt gcgccttctt gccccactgt ttgttcgatg tcactttgca atccgtctac
     9241 tgttacacct tttggaaaac ggaaattcat attgatgaag ttcgcttcct ctgtaccatt
     9301 ttcttcaaaa gcaaatagtc cagcattcat tgtcaatttg cccatttttt catcttcata
     9361 agcgactcct agtttttctc cataaaaatc ttcatggaca taagcagctg ctacatggat
     9421 aaattgttta gcagatccta ggaattcata gttatctaag aacaatgcca agaaagaacc
     9481 tgcattgata ccagattgtg ggcttgcacc atgtgcgcct ttaccaacta attcgatttt
     9541 tacatacgta ttgtccgctt caatcgttcc gcttactgga tttgcttcga tgaattggta
     9601 aaatgcttct tccatcgcga tagctgcatc tgcacttggt acttgaagtg ctgcagttgc
     9661 tgttcctggc accatatttt cgcgaagtcc ggaaacaaaa gatttcaaga cataatctcc
     9721 tgcattgtcg cctttgaagc gtaaagctaa agaaacattt cctttttcac cattgatgat
     9781 tgggaattca gcatctggtg agaaaccaaa atctggttct tcttcatgtt cgaaataata
     9841 agccatatcg ccccagccgc tttcttcatc gctgccgact acgaagcgaa ctttttttga
     9901 tagtttcaag tcaagttctt taatgatctt catcgcatag taagctgcca tacttggacc
     9961 tttgtcatcg cttgatccgc gtgcaaagat ctttccatcc ttgattactg gttcataagg
    10021 atctgtgtcc cagccatctc ctgcaggaac gacatccatg tgtccaaata tccctagcgt
    10081 ttcgtctcct tcgccaagat caatatgccc tgcatagtta tcgacatttt ttactttaaa
    10141 tccatcacgt tcgccataag ctaacatatg tttcaatgcg tcgcgaggtc caggtccaaa
    10201 tggtgcatct ggtgttactt ttgagtcatc gcgctcgctg ttgacacgta aaagattttt
    10261 caagtcttca agcaagtctt ctttacgtgc ttctacttct ttttgccaat caattgtcat
    10321 aaagttaatc ctccacttat ttaagtcttc gtgttcatga taccacaaat tacagaaaaa
    10381 aatagttcaa aaaatctgac aattcctttt cttatctgtt tttctctaaa aaaaatatac
    10441 gctataatga gtaaaaggag gtttttagat gctgataaac gaacaactag tgaaaagagt
    10501 catcacacct cgtccaaaaa tttcacataa aggaacattt ggacgtatcg ttttgattgg
    10561 cggcaatgaa caatatggcg gagcaatcat tatgagtgct ttagctgccg tacacagcgg
    10621 agcgggtctc acgactgtag taactgatga aaaaaatcgg agtgctcttc acagtcatct
    10681 gccagaagca atgattgtag tttggacgga tactgaccag tttctttccg taataaagca
    10741 agcagatgta ctcttgattg gtcccggatt gggtacttct tctaagagtt tggaacgttt
    10801 caaacaagta ctagaaaacc aatcaaaaga acaatggctg gtgattgatg gctctgcttt
    10861 aagtttactt gctgctaatc atcttttgct accctatccc gaacaaactg tacttacacc
    10921 tcatcaaatg gagtggcaac ggttgagttc tctaccgata caagaacaac gggataaaga
    10981 aaacaaaaag aagcaacagg aaatcggtgc tgtgctcgtt ttgaaaagtc atgagacaaa
    11041 aatttatggt caccagcact tctacaccaa tcctttaggt tctgctgcta tggcaacggg
    11101 cggcatgggt gacactttag ctgggatgat cacaggattt ttagcccagt ttcctcaaaa
    11161 tgaccaaacg attgcagcag ctatttatct tcatagtttc attggagacc agctggcaaa
    11221 agaacaatat attgttttgc cgacacaaat cagcgaagca cttccgaaat ggatgaatta
    11281 tttttgtaaa aaatcaatgg aaaacgaggc atgagtcatg gagaaattta caggttatgt
    11341 gcgagaacct ttttattatg aaacagatca aatggggatt attcatcaca gcaattatat
    11401 ccgctggttt gaagaggctc gggtagcctt attggtacat ctagggtttc cctataaaaa
    11461 aatcgaagaa tctgggatca tcatccccgt tctggaagta aattgcagct ataaagaaat
    11521 gatccattac ggagaaaaag tgagcatcca tccaactatc caaaagtata cggggacacg
    11581 gttggatttt agctacgaaa ttgtgggaac tgaagacaaa aaattaaaaa ctactggcag
    11641 tagtaaacac tgttttttac tttctgattc gcaacgatta gtgaagctga gcaaagtcaa
    11701 tccggagtta gatcagttat tccaagcgta cgcttctttg gatgatgtaa attcttaata
    11761 aagaccgtct aagcattttt cttgtattgt gtcgagtagt tcgtataatg aaaagaaaaa
    11821 agcaggtgat ccccatgtat gtcgttaaag taatgcacgg ttacatcgat aaaactggct
    11881 gtcgcactcg ggaaaaaaac ttagacaatc tgctcatttt caaagacaaa aaagaatccg
    11941 aagcttttgc taaaagaatc ggcggtcgtg tcaaaccgat ccaagaggta cgaccagatt
    12001 gattcaatat ttttcttttc gaggctctaa taggcatgcc tatcagggct ttttttgcag
    12061 actgtgtttt tatgcccaca cgatttgtaa gcgatttatg ttataataga ttatatctgc
    12121 atagaaagga aggttaaatg gttatcaata atttgcaaca gcaagcttat gaatctatac
    12181 gtaaacaaat catttattcc gagttagcac ctggaagtaa aatctccgat aaagtgttag
    12241 aagaacaact agctatcggg cgtacaccga ttcaagaagc actgatccaa ctgcgcaatc
    12301 aagagcttgt aaaaaccatt ccacagtccg gcacttatgt ttcaaaaatc gatatccgat
    12361 ctgcttcatt agcccgttat acgcgggaaa agttggaaaa accgatcctg caagaatgca
    12421 gtgcgaaaat gaccaagcaa gatcaagcta aactagaaaa aatactagaa caaaccgatc
    12481 aggcagtaat cgctcaaaat aaaaaagctt tttttgattt ggatactgat tttcaccgaa
    12541 cttgttatga aattgctgga aaacgagaaa tttgggattg gctagaaagt tatagtacgc
    12601 acttgaaccg tttcagatgg ttacgtttga cgatttcaga attagactgg ggtcgagtat
    12661 tagatgaaca tcagacaatg cttcaatcaa tgattgatca taactttgat gaagtcggat
    12721 ttctttgcac gatgcattta catatgatta tcgaggagca agaatacgtg attcataatt
    12781 accccgatta ttttgaagat attcaggaca gacccatcaa ataagaggtt ttggaatcag
    12841 cgttttgacc tgagtattag agaaagaaac ggcaaaatag ttcctcatat tttgccgttt
    12901 tttttgagct aataccgagg gtcagctgat tgcacaccgt ttattgagag gttgtgaaaa
    12961 agctgccctg catcaagtaa taagaacagc caagtaaaaa tagcttctta tgtttttaac
    13021 ttggctggac ttattaccgc agatgcaagc ttttgaacac cgttttccaa gaggtcgtga
    13081 gcctgccgtt catctccgag tcacaaggaa caatttcaaa aaacagcttc tcatgtttgt
    13141 tgaatatttt gacttgtgac cgaggagttg acaggagaac atcgtttatt cggaaaaaaa
    13201 ggactgtgac acgacttttg tcacagtctc tttatttgta atctaaatca tactccgtac
    13261 atcttttttc tatttttttt atttcttcta agatcccttg gctacgaatt tcattatatg
    13321 gccaaagtaa gactgcctct aatacataag gcgaaaaatc gctttgattc atctcttttg
    13381 aaaaactcat cgctgctatc tcgctcaatg cacgaatgtt tgattgatgt gtataaaaag
    13441 gcaaccgcca cagcacaatt ttttcgcttt gcgtataaat tcccttaaca gaagcttcga
    13501 tgtggtgaaa tagttcatgt ccaataatca aagcttgcgc tgtttcttta gagatcgaag
    13561 ctagtcctga acattttaat ttttctaacg gctccgtcat caatcgaatc ccttcatctt
    13621 ctgcataagt tgcaaaagtg attcgttcgt ttatttgctc tgattcacag gaaacgattg
    13681 gaacagccat ttgttcacaa atttctccta catcggcaaa agggtacttt tttttgatgc
    13741 tacatgccgt ttcgatacca caatcgattg attgttgtat aagaattttc tgcttggaca
    13801 aaggaatctt tttccaaaat aaatctcgcc gaaactggta gaatccccaa atttcttcgg
    13861 ggatctgttg taattcgttt agtaactctt ctaacttatg tttgtttgat atttccggca
    13921 acgacgatca cttcttcctc cggttccata gtagatactt ccagatccat caaaagctgt
    13981 agttgttcca gatccgtcgc tgtaaaatca gagactcttg ctcctaagca gagataaaat
    14041 tctggtcggg caatcaattc agtttgataa actgccatat cttcccaaag ttgttcgaca
    14101 gctgtcatat aattggctgc agttgttttc atacatgaag catgtccacg ttggaccttg
    14161 ataaatcctt tttgatcgat gatccgaaga cttccagcat tttttgaacg attctgttcg
    14221 ccgcatacat aaaagaaagg tgtattttcc aataagcaaa cttcattttt atttaatcgc
    14281 atatcctctg cagcaagctc tagtgcttct tctgttgtga tctccttggt tagatcggtt
    14341 gctttcactt ccgtagaacc agtagcgata gccgttactt ttgaagtctg tggatcgatt
    14401 tctacgtgga cttcgatcga ttctggtgta gcgcctgatt caatcgcttt attcatcgct
    14461 tcatttttca acgatcgtat atcttcttta cttggcgaag ggatgatacg ttctacaaca
    14521 tctcggacca ttgcaagtgc tacaccaata gaagaaatca cttcggcgtt ttcaggaatc
    14581 gaatatttca cacccatttt attactaaag taagtaatca atgaggcagc gccgccccct
    14641 acacctacaa gagaaatctg atctttctcc aagtgatact tgtctgccaa ttctaaaatc
    14701 accggttcaa tcttagcata ggatttttcc atgatctgtt ctgcgatgtc ttcaaccgtc
    14761 gtatgacaat aatcagctaa tgcttggatg gcttttcgtg cagatgggac atttccataa
    14821 gaaaaatgtt cttcttgcac taatccaagg acattggctg cacatgtatt tgtaatagtc
    14881 acttcttctc cgtcttccat cactaccttg acgtaatcag cagggtctcc ttctttagga
    14941 gagaaaaatt caaccttagg acctttgatt ttttctgtct ccgtaaatac tgcataatct
    15001 agaccggcaa tatgtgcaga acgtggaccg acatcgatga ttcctgactg attagcccgt
    15061 accatcgaac cgcctgccac acctagaacc cgaacatcta atgaagagat atatgttggg
    15121 tggccaccta caattgaata atcgattgct ggacgtccat ttttaatgac cccgatattg
    15181 gttgttgttc cgcctacttc aaaataaaca ccattagacg ctctcaagta catcaaagag
    15241 cccatgactg atgctgctgg accggaaagc atggttaaaa caggtcgttt tttcatttca
    15301 ttgatttcca ttactccgcc gtctccgcgc ataatcatca aagaaacatt gacccctgct
    15361 tctcgtacgg aatcttctgt agaggttgcc gtatctagca tttttggtaa aatagatgca
    15421 ttgattgctg ctgtccgagt tcttcttgtc aatccatata attttgtgat gtcagaagcc
    15481 atcgttgtag ggatcgattt taccgatgct gcttcataaa caactcgttc tggttcccca
    15541 ttatctactc caaatgccat agaagaaacc aagacctccg ctctttctct ttctaaagaa
    15601 gaaatcgttt ctgaaacacg gtctacattt aagtgtttga caggtaagaa tgcattgaca
    15661 atctctattt ttttcttatt ccctaaatcg atatcgttca atctagtttg tcgttttgct
    15721 aaaaaacctt ctaatccacc ttttgccatc ccgattacgc cgacttttgc tacgtctcct
    15781 tcaatcagtg cattagtcgc ttgtgtggta gaatgtgcaa caaacacaac atcttctgga
    15841 ctgatattat tttccagcaa acaattttga aaagactgaa cgacacctgc ggctactcca
    15901 cggacatcat cgtgggttgt tttcactgaa gattttccga tgatttcgtg cgtcgcatta
    15961 tcgattgcga ctgctttcgt atgtgtaccg ccaacatcaa ttcccattcg cacaaaacgt
    16021 ttcattttct tttcctccat tcatctaatc gtttttatcg acgttttgtt tactaggcgt
    16081 tcccgtacaa taagaacgtg atcaggcata agatcgctcc gatgatccaa ccagttggga
    16141 tggatttctt catatattct cgagaagtta cttttgtata acctaatccc caagcaaccc
    16201 aagattgtgt aatatctaaa tgctgaggag caatcgttgt gattgcaaat aacgggaaca
    16261 aaaaggctgg cgacatcgtt ggattaacag ctaatacgac agctaaaata gctgagcctg
    16321 aaccgactag attcattgga ccgcggaaaa atcctagtgg tgtcaatata gcaaatacga
    16381 ttgccaagac taatggtgat tgcggtatcc aatctccaaa aatagctgaa aaataaggag
    16441 aagcatacgc agctgaatta ttgaacatcg ctaaggtcat caagaaacca accattggcg
    16501 caacatctac tgcgccatct gtaaataatt tcgcaaactg acgacagatt tctttataac
    16561 ctccgcgcaa ttttccagta aacagcaatg cccagatacc tgctagaata aaacctaaga
    16621 tgattggaag atctaaaaga acgaccccta aaactggtaa taatacggct aaccatgaga
    16681 tcattggtgc gtcgccacct tcagctggta cgtttgctgc cattgcatag cgtttctttt
    16741 tgttcattga tatattagcg accgttagta ccacaactaa gcttacaatc atcccaatca
    16801 taccaatttg gaaatagtca ttataagtat agctttcagc cgctggattg aaaccagcat
    16861 aaatcgtttg atattgtttg aaattgacga tattagcaaa gattccagcc ataatcgagc
    16921 ccataaatga gaacagtgca accggtactg aaatacctaa tgacatcaag attggcaaca
    16981 caataactgc aatagaaatc actggtccga ttcctgtcat cgacatgaat ataacagcgg
    17041 ttacaataca tagtaagctc atcgtgattc ttggcttgtc ccctccaagt tcaactacct
    17101 ttcgaatcag ggtagctgcg attccgctat ctactagaac acgcccgaaa aaggcaccaa
    17161 agaagacatt taccaagatc gatttggcgt actcagctgg ccccgtcgta tagatatgcg
    17221 tcaacacatc aattactcct tgcccttcca tcgccgggtt tggtgaaaac gtgtttccga
    17281 ctagcgctaa tcccatccat acagtagcca tgatggcaaa gccgaccatc aagttgtagc
    17341 cttttacaca gtaccagacc atgaaaaaga atgagataac taacaaaata ccaataataa
    17401 tatcactcat gttattcctc cgtccatttt gtttttctga taagcaaact aatatatcag
    17461 tcaaactcat tctaacttat gtagttagcg ctttcattaa agggtgacca taaatgttca
    17521 gttttcaatt aaaaaacctt aattatgcta ttttagactt atacgttatt gtcctgtttc
    17581 atttgatgga ggattgtaaa taaaaaaaga ggaaagaaca taacttttcg ttatcttctt
    17641 tcctctttat aattgtttag cttatttagc caaagcttct tttgcttggt cagccaatgc
    17701 agtaaatgct gctgcatcgt taacagccaa gtcagctaac attttacggt tgatgtcgat
    17761 ttcagcaact ttcaaaccgt gcattaattt tgagtagctc aagccattca tacgagctgc
    17821 tgcgttgata cgagcaatcc acaatttacg gaagtcgcgt ttcttttgac gacgatcgcg
    17881 atatgcgtag ttgtatgaat tcattacttg ttcttttgct gttttaaata aagtgtgttt
    17941 tgaaccgtag taaccttttg ctaattttag cactctttta cgacgtttac gagttactgt
    18001 tccaccttta acacgtgcca tgtataattc ctcctaataa aaataaatct tcagattctg
    18061 ttatttgtac ttcttatttc atttttgcta gttgttggcg aatacgtttg aagtcgccac
    18121 tagaaaccat gcttgcttta cgcaattgac ggcgttgttt ttttgttttg ccgtggaaac
    18181 ggtgacttgt aaacgcgcgg aagcgtttca atccgccttt accagtacgt ttgaaacgtt
    18241 ttgctgatcc gcggtgtgtt ttttgttttg gcatgactat atttcctcct caaaatcttt
    18301 cgttatccga ctatcggctg tccaacagcc agttagtcat tcaaattact tactgtcgtt
    18361 tttcggtgcc agcgtcaaga acatgctgcg tccgtccatt ttcgcttttt gttccactgt
    18421 tgcaatatcc gcagtttctt cagctaagcg attcaagact ttttgaccaa tttctttatg
    18481 ggtaatggca cggcctttga aacggataga agctttcact ttatcgccct tttccaagaa
    18541 cttacgtgca ttacgaagtt ttgtattgaa gtcattcaag tcgatcgtag gacttaagcg
    18601 aacttctttt acattgatca ctttttgttt tttacgcgct tcgcggtctt tcttttgttg
    18661 ttcgaaacgg tatttaccgt agtccatgat tcgcgcaact ggtggtttcg caccaggggc
    18721 aactaacaca agatccaagt ttgcttgttc tgcaatattc aatgcttctg cttttgtctt
    18781 aactcctaat tgctcaccat ctgaaccgat caatcgtaac tctcgtgcac gaatgccgtc
    18841 gttaaccatc atatcctttg ctatggtcat tcacctccaa atattatgag agaaagaaaa
    18901 gcttaccgaa taaacggcaa taaaaaaacg agttctctta cagaacccgc gcgtacttca
    18961 cctaatagaa ccagtgaaaa ctatctagcc cagtgacatt atcaactaag gcgagaagcg
    19021 ggtgcttctg cttttatttc tcaaccatca tagtatagca aaaactaatc tttttgtcaa
    19081 tgtttttcat acagtttgtc ttccttttct gaaaatgatt agattagcag actttcttgc
    19141 agtgattccc gcttctttgc tatactaaag aaaaacgagg tgaacccaca tgaaaaaaat
    19201 cgaacctatt ccaccttctc ttcctcttct tacaaaggga gagttttatt tagaagatga
    19261 ggagatcttc tctggtcttc tgatcaacgg gggtgaccaa agctatctcg atgccaaaaa
    19321 tatggtatta agagagagtc atatccaaaa acttacgatg caaaaaacag ttttacgtca
    19381 ttttgaatgc agtaatgtca tttttgaaaa atgtgatttt tccaatctgg agtggattgg
    19441 tggcagtttc caccaagtcg tgtttcatca atgtaaatta acaggtacga attttgccga
    19501 aagttatctt cgagactgta ctttcactga ttgtattgct tcgatggctt cctttagtag
    19561 tacaaacttg aaagctgtct ccttcgatca ttgtcaatta gaagacagcg aattttatga
    19621 ggtcacttgg aaaaaccttt ttttgtctga caatcagttg accggttcga actggtttcg
    19681 aacttctctg aagggattag actttacgac taatttcttc acagcaatcg ctttatctca
    19741 agattctttg gcgggactaa tcgtcaacca agagcaagca cttgtgatcg cccaagccct
    19801 tggtttgcag atcaaagact agatcaacga atccgccgcc acagtgcaat aagcatccaa
    19861 atagataaga ccaaactgat gatcaaggcg attccccatc cgaaaacgga atgggtaaag
    19921 ggtaactgga gattcattcc gaagaatcct gtcacaatag ttggaacggt caagatcagc
    19981 gaccagaccg tcaaaaattt catcgtgtca ttcaagttgt tattcagcaa attattataa
    20041 gtgccagaga gctggtctaa aatctgcgaa gctaagttcg tcatttcaac tgcttgtcgt
    20101 gcttcgatca gcgcatcgtc caactcttct ttttcctttt ctgacaactt gcgataaata
    20161 gccaatgcct tgatttgttc taaaagtact acattttgtt ttgttcctgt aactaagtaa
    20221 accaaaccga tctctaaatc tgaaagttgc agcagattct tgttcgttgt tttttctctt
    20281 aatttcagat taagccgctg acgttcacta ttcacttcct cgattaaagg aaaaaaggaa
    20341 tcagaaatca aaaacaatgt acgaaacaat aaactgtata cactcatgtc tggcattcgt
    20401 tccaaaagcg atcgaattag tcgaatcacg tactcggtat tcggcgtggt aaatgtaaac
    20461 aaaccgtctt cttttaaaat aaaagcgata ggactagttt cataatgatt atcgactttt
    20521 tgctggtgcg gaacatttga tacaagcagt aacgcttcat caaaagaatc gtactctacg
    20581 cgcgcccgtt catttttatc tagagaataa gtcagcatct cgtcactaat gccatattct
    20641 tcttttaagt ttttaacagc tagcacatcg ttttgctgta cattgatcca aaaattacgg
    20701 tcttgattaa aatagtgttt ttcccgcata ctctatcctc ctgacatttg tttccagtat
    20761 aaagaaaaaa gtcgctcaaa taaaggttta ccctttattt gagcaactgt aaatcaaatg
    20821 gctactttgg tttggcgaat ctctgctaat ttttcttcga tcattttcaa gacttcttct
    20881 tttgcttgag gatcttccac aaaattatag cggtctccat cgatttggat ttttggactt
    20941 tctttgtatt cttcatacca ttgatcgtaa cgtctgttca attctttgta ataatcgtat
    21001 agacttggat caaaggacaa ttgttcatac gaacgaccac gtttttcgat acgctccaac
    21061 atcgtatcaa aagatacacg aatatgaact aataaatccg gatgtttttt atgggcagca
    21121 tagggaagtt cttccatcat gttttccagc agttcatcat acacacgaac ttctgtttct
    21181 gttgctcttc ctagatctgc atttagatgg aaaagcagtg aatcttcata aatcgaacgg
    21241 tccaatacat tattgtcatc tttcattgct tgtttgatgc ttgcaaaacg tttattcaaa
    21301 aaataaattt gcagcaaaaa agcatactgt tcagggtttg cataaaataa gggcaagact
    21361 tcattatcat caatcgattc ataaaatgcc tgactatcaa agtgttcagc catcatttct
    21421 gttaaacttg attttccggc accaattgta cctgctaaaa cgatcacgct catttacctc
    21481 cagctcactt catcactaaa tattgtgttt aactccaacc tttttaaata tacccccaat
    21541 atctagtagt gtcaattgta tttttttcta gtcaaagaag aaaaaaagtc aaaaaattct
    21601 taagaaaaaa gtgttttttc tttagaaaac gtattcttag ttttacttct gagatggttt
    21661 tagtaaaata aaggaggaat gttttttgag acccttagtg gacaaaaaaa ttcgtgaagc
    21721 agcgcaactc gttttgggcg ctttattttt tgcttaaaaa tcacttttac gtgagaagga
    21781 gttagattat ggcaaactat ggccaaggag aatcaagcgg aaagatcata ttgatgggcg
    21841 agcacgcggt tgtttatgga gaaccagcga ttgcctttcc tttctatgca acaaaagtca
    21901 ccgcattcct tgaagagctg gatgcaatgg acgatcaact ggtttcttcc tactattcag
    21961 gaaatttagc cgaagctcct catgcattaa aaaatatcaa aaaattattc attcacttaa
    22021 aaaaacagca tgacatccaa aaaaacttgc aactgaccat tgaaagcacg attcctgctg
    22081 aacgtggaat gggatcaagc gctgcagtcg ccacagcagt cactcgtgct ttttatgatt
    22141 acttagcatt tcctttgtct cgtgaaatac tattagaaaa tgtccagctt tcggaaaaaa
    22201 tcgcccatgg taatcctagt ggaatcgatg cagccgctac tagcagcttg cagccgattt
    22261 attttacaaa agggcatcct ttcgactact tttctttgaa catcgatgct tttttgattg
    22321 tcgctgatac aggaatcaaa ggacaaacaa gagaagccgt caaagatgtc gctcacctct
    22381 ttgaaaccca gcctcatgaa actggacaaa tgatccaaaa attaggatac ttgacgaagc
    22441 aagcaaaaca agcaatcatt gaaaattcac cagaaacgtt ggcacagaca atggatgaat
    22501 cacaatcact tctggaaaag ctgacaatca gcaatgattt tcttaatcta ttgatccaaa
    22561 cagcaaaaga taccggggca ttgggtgcca aattaactgg cggtggacgc ggtggctgta
    22621 tgattgcatt agcacaaaca aaaacaaaag cccaagaaat cagtcaagca cttgaagatg
    22681 cgggtgctgc tgaaacttgg atacaaggat taggagtaca tacctatgtt taaaggcaaa
    22741 gcacgcgcat ataccaacat tgctctaatc aaatattggg gtaagaaaaa tgaagaactc
    22801 atccttccaa tgaacaacag cctttcgtta acattggatg cattttatac agagacagaa
    22861 gtcatctttt ctgatagcta tatggtagat gaattttatc tagatggcac cttgcaagac
    22921 gaaaaagcaa caaaaaaagt cagtcagttt cttgaccttt ttcgtaaaga agccggcctc
    22981 tcactaaaag cttcagtaat cagtcagaat ttcgttccta ccgcagctgg attagcctcc
    23041 tctgccagcg ggctagctgc tttagcaggc gcttgcaata ctgctcttaa gcttggatta
    23101 gatgatctct ctctttcaag atttgctcga cgcgggtctg gttcagcttg ccgaagtatt
    23161 ttcggtggtt tcgtcgaatg ggaaaaaggc catgacgact taagttctta cgctaagcca
    23221 gtcccttccg attctttcga agacgattta gcaatggttt tcgttttgat caacgaccag
    23281 aaaaaagaag tgtccagcag aaatgggatg cgtcggacag tcgaaacatc cagtttttat
    23341 caaggctggt tagattccgt tgaaggggat ctatatcaat tgaaacaagc aatcaaaaca
    23401 aaagatttcc aacttctcgg agaaacgatg gaaagaaacg gactaaaaat gcatggaacg
    23461 actttagctg cccagcctcc attcacttac tggtctcctg acagcttaaa agcgatggat
    23521 gcggtgcgac aattgagaaa gcaaggcatt ccatgctact ttacgatgga tgctggccct
    23581 aatgtcaaag ttttagtgga aaacagccat ttatctgagg tacaagaaac atttaccaaa
    23641 ttatttagca aagaacaagt aattactgca catgctggtc caggaattgc gattattgaa
    23701 taaagggaga tttttatgat tgaagtatct gcaccaggca aactctatat tgccggagaa
    23761 tatgcagttg ttgaaacagg ccatccagca gttatcgctg cagtcgatca attcgtgaca
    23821 gtcactgtag aatctgcacg aaaagtcgga agtatccaat ctgcacaata tagtgggatg
    23881 cctgtacgtt ggacaagacg caacggagaa ttagttttag atattcggga aaatcctttt
    23941 cattatatcc ttgctgctat tcgcttgact gaaaagtatg cacaagaaaa aaacattctt
    24001 ttatcctttt atgatctgaa agtaacaagt gagttagaca gttcaaatgg ccggaaatat
    24061 ggattgggtt ctagcggtgc cgtgaccgtt gcaacggtca aagccttgaa tgttttttac
    24121 gcgttgaatt tatctcagtt ggagattttc aagattgcag cactagccaa tttagcagtt
    24181 caagataatg gttcctgcgg cgacatcgct gctagctgtt atggtggctg gatcgctttc
    24241 tcaaccttcg accatccttg gctccaagaa caagaaactc agcattctat cagtgagtta
    24301 cttgccctgg attggccagg tctatccatt gagccattga ttgctcctga agatttacgt
    24361 ttattgattg gttggacggg tagccctgcc tctacttctg atttggtcga tcaagttcac
    24421 cgttcgagag aagataaaat ggtggcttat cagcttttct taaaaaatag tacagaatgt
    24481 gtcaacgaaa tgatcaaagg gtttaaagaa aataatgtaa cgttgatcca acagatgatt
    24541 cgaaaaaacc gacaattact gcatgattta tctgcaatca ctggggtcgt catcgaaacg
    24601 cctgctttga acaaattgtg taatttagct gaacagtatg aaggagccgc aaaatcttct
    24661 ggtgcaggtg ggggcgattg cggaatcgta attgttgacc agaaatctgg cattcttcct
    24721 ttaatgagtg catgggaaaa agcagaaatc actccactgc cgttacatgt ctatagcgat
    24781 caaagaaagg aaaaccgatg aatcgaaaag acgaacatgt ttcactagct aaagcctttc
    24841 atgataaaca aaaaaacgaa tttgacttcg tacggatcat ccataaccct ttgccgcaaa
    24901 tagcagtttc tgatgtcgat ctaagtacac aggcagttgg gtttacacta agcagcccat
    24961 tttatatcaa tgcgatgact ggcggaagcg aaaaaacaaa aaaaatcaac caagatcttg
    25021 caatcgtcgc aagagaagca gatttgatga ttgccactgg ttcagtcagc gctgccttga
    25081 aagtcccttc tttagccgat acttatacga ttatgcgaca agaatatcct cacgggaaaa
    25141 tcattgccaa catcggtgca gggacttctg tcgaaagagc caaagaagct atccgattgt
    25201 ttcacgcaga tgccttacag attcatttga atgcacctca agagttggtt atgcctgagg
    25261 gagaccgtga ttttaccaat tggaaagtac ttattcaaga aactcaaaca gccattgacg
    25321 tccccctcat cgtaaaagaa gtcggttttg gcatgacaag agaaacgctc aatgatctag
    25381 ctgctttagg agttcataca gttgatatca gcggtcgaag cggaacaagc ttcacccaga
    25441 tcgaaaacgc ccgtcgctcc aagcgggaac tgagttattt agctgactgg gggcaatcga
    25501 cagtctcttc attgcttgaa gcaaatgaag cagacacttc tatggaaatc ctagcctccg
    25561 gaggtatccg caatgcctat gacatattta aagccctttg tctcggcgca aatgccgtgg
    25621 gtacttcagg aaccgtactg acgcatctga tgaatcacgg cgtagaagaa actattatac
    25681 tgatgaagca atggcaagaa gagcttcgtc tgctttatac tatggtcggc gcaacgaata
    25741 ccgcggccct acaccaacaa tctctgatct tttccggacc agtgaaggat tggtgcgaag
    25801 caagagggat cgacttagtg aagtatggaa gacgcacaga aaagagcgtg ggacaaaagt
    25861 aaagaacact tttgtctcac gctctaaaca cgtacaaacg gcggaagtaa aagcaactcc
    25921 ttcggaaata agctaaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa ttccttctta
    25981 tttcttggag ttaaatgctt ctgtcccagc ctcttttttc gaataaatga tgtttaatca
    26041 gctgtccttc ggtattcgtt caatccgctg atttcacaac ctctccacta aacagtgtac
    26101 taaagctctt ttaaggttgt tttccgatat aggcaagtat gcctccgtcc acatacaaga
    26161 catggccgtt aacgaaatca gatgcgctag aagccaagaa gacagatggt ccagccaaat
    26221 cgattggctc gccccagcgt gctgctggcg tacgtccaac gataaattga tcgaatggat
    26281 gacgttcacc gttttcttgt gtttcgcgta atggagcagt ttgcggtgta gcaatataac
    26341 ctggtccaat tccattacat tggatattgt attgaccgta ttcagaagcg atgtttttcg
    26401 tcagcatttt taatccgcct tttgccgcag cataagcact gaccgtttcg cgtcctaatt
    26461 cactcatcat ggagcagata ttgatgattt taccgccgcc tttttcgatc atatccggaa
    26521 taactgcttt tgaaacgatg aatggggcat ttaaatccac gtcgattact tggcggaagt
    26581 ctgccgcatc catttcgatc atcggtgtcc gtttgatgat ccctgcattg ttgactagaa
    26641 tatcaactga acctacatct tctttgatct gttgaaccat cgcttgaacc gcttcttcat
    26701 cggtcacatc gcagacataa ccttttgcat cgatgcctgc ttctttataa tgcgctatac
    26761 cttcatcaac tgattcttga ttcaagttat taaatacgat ttttgctcct gctgtagcta
    26821 aagagcgtgc aatctcaaat ccaataccat agacagcacc ggtgactaat gctacttttc
    26881 catctaaacg aaacatatcc atactaaatt ccatcatttt ctctccctta tgcttatttt
    26941 aattgatcca tttgcaccat atccatgtct gtataggtga tattttctcc gcacattgcc
    27001 cagatgaatg agtaattgct tgtacctaca cctgaatgga tcgaccagct tggcgaaata
    27061 atggcttgtt cattgtctac aactaaatgt tttgtttcat ctggtttgcc catcatgtgg
    27121 aacacacgtg tatcttcatt ggcataatca aagtagacat aagcttccat acgacgttca
    27181 tgtgtatgac aaggcatcgt attccaagcg cttcctggtt ctaaaatcgt ataacccatt
    27241 tgtaattgac agctttcgca tacatttgga tggatgtatt ggtagatttt acgttcattc
    27301 agtgtcaggc cttcaccagt ctccattggt tttacttggt cgatactgat ttttacattt
    27361 ggatatttat gatgagctgg aacagaacta atatagaatt tagctgggtt ttcagcatct
    27421 tgagaagaaa atacaacatg tcgtgtttct tttccgatat agtatccgtc ttgttttttc
    27481 atttcttctt tttgcccatc gatctcgatg aatcctggtc caccgatatt gatcacacct
    27541 agttcacgac gctctaagaa gtaatcaaca cctagttctt tgtctaatgt gatttctaat
    27601 tctttttcag taggtgtcac tccaccaaag atcattcgat cgttgtgcgt ataagtcaaa
    27661 ctgattttcc ctggttcaaa taccttttct actaaaaatt cttttctcaa ttgttccgtt
    27721 gaataatgac ggatatcttc tggactgtgt gtataacgtg tttccatttc ttgcattttc
    27781 ttctactcct tctatcgaat gatttttcca gagccgcatg ctaaaaattg tgcgacttct
    27841 tcttgactaa attggttgca gtccccatgg atcgtatgct tcaaggcaga agctgccgta
    27901 gcaaaatcaa tgatttcttg cggagacata ttggataaaa tcccatggag cacgccacct
    27961 gcaaaagcat ctccgccacc tactcgatcc acgattgggg tgattctatg gacttgggat
    28021 tccacatatt catggttcat ccacaatgtc cccttcaatt cattttcact tgctgaatga
    28081 acggtacgct tggtcgaata aaacacctga atattcggga acttttcctg cattttttga
    28141 taataataaa ttagaggttg ttcttctgct tctggcgctt tctcgatacc taacaaataa
    28201 agagcatcca tttttccagc tgagcagata tctactaaag gtaaaagctg atggattact
    28261 tcgctggctt cttgctgtgt ccataatttt ccacgatagt tgatatcgaa gctgatcagg
    28321 cagccagctt tttttgcagc ttctatcacc tgtttcgtca ttttttgcca ttttttagac
    28381 aaagctgggg tgattcctga aacatgaaat atatctattc cttggaataa ctcctctagc
    28441 gaccattcca aatgatccat ttcagcaaaa cttgatccgg cacgatcata gatcactgag
    28501 gcggcgcgtt cgccactacc agtttccata taataagtac ctagtcttga tccaccagat
    28561 aatacctgtt tcgtatctac tccataacat tgcaagtgct ttttgactgc ttttcccaat
    28621 gcgttatcag gtacttttgt agcaaaaatc gcttcatgtc cgtaattagc taaagaaatt
    28681 gctacgtttg cttcccctcc gccataatgg gcagcgaatc gttcacttga atcgatacgg
    28741 gttccctgct ctgtggacaa tcgcaacatg atttctccga gtgtcactac ttttcccata
    28801 agcagctacc tcttgatttc tttcattttt gctgcatact gttgcgcgac ttccgttact
    28861 ttgtcaaaat ctcctgtagc tgcgggtgct aaaaggttcc cacctactcc gactgttacg
    28921 acaccagcat cgaaccactc tttcatattg tccagactca caccgccagt tggcatgata
    28981 ttgacttgag gcatcggcgc cttgaaggca gaaatgatac ttggtccata agcgcttcca
    29041 ggaaatagtt tgacgatatc taccccgctt ttcagcgctg ttttgatctc ggtaatagtc
    29101 atacatcctg gtaagtaagg aatttgatac agattgcaca tctctgccgt ttcttgatca
    29161 aaggaagggc taacaatata ctctgcgcca gctaagatag ccaagcgagc tgtgacagcg
    29221 tctaatactg tgcctgcgcc aatcactatt tctggttggt ctttgtagaa tgacaaaagt
    29281 tctttgatta cttgatctgc ttgagggact gtaaaagtca actcaatacc agttagacca
    29341 cctttgatga ttgcgtggca agcattcaaa gcttcttctt tgctttttcc gcgtaccacc
    29401 gcaatcactc cggcgttttt caatcgagat aaaatatcaa tttttttcat cttctccacc
    29461 tatttcctat ttagaaattt tattttataa cttggaaatc tatacccata ttatacggca
    29521 gaaaagaaaa attgcaaacg cttttttcat tatttttgaa ttctttgctc gatttcgctt
    29581 tttgtttgct tgagggcact gattatttgt tcttttgtct catctgttaa acgatatttc
    29641 ggcatactta cactaaatgc accaatgatt tgttcgtttt tcacgattga ggctcctata
    29701 caaaaaatat cacgttcgat ttcttcatcg tcaaatgcga tgtgggtttc ttttaccgtt
    29761 ttgatttcct ttttcaaccg taatgcattg gtgatcgtat attctgtgta agggatcaac
    29821 ggatgctttt caagataatc agtgtactgc tcttcactga attctgctaa tacggctttg
    29881 cccatcgcag aattgtataa tggtcgggtg attccgattt ttgaagacat gcggatcgtt
    29941 tgatgttttg gatctagttt gttcacgtac aagatctcat tgttgtccaa tacccctaag
    30001 tggatagtct catcgatctt ttgctgcagc tgttctaaaa acggtaaagt caactctacc
    30061 aaatcgattt gttcaatgtt tttattcgcg tagcgaataa gctttgcacc taaacgatag
    30121 tttttctctg aatttttatt tacgtagccg atcatcaaca aagtatctag tattttcaag
    30181 actgtagaag aggtcattcc cacacccttt gcgatcgtct gcaaagagac atcttggttt
    30241 tccgctaaat agtctagtat tttagaggct ttgatcaata cagttccata aggcttttta
    30301 ttttccatat aatattctcc tatttcttta taagaaaaat tatagcggaa ataccatcaa
    30361 tttaaaagag atatccagat ataaactacc atttctcttt acaggcaatt taatgttgaa
    30421 gatccgcttc gtttttacat caaactttga tataaagcaa tgatttcttc tactgtcgtt
    30481 tctcttggat ttcctggtgt gcatacatct cgcaaagcat cttctgcaat tgctggaata
    30541 tctgcttctt ttacacctaa ttcagaaatg gtttctggga ttccaaccgc tttgctcaac
    30601 cgatcgatct caccacaagc cgcttctctt acatcttcta aagacatttc agctgctcct
    30661 ttaatcccta gaacaagagc gatctcacgg tacttttctc cagtatattc tttgttatat
    30721 ttcatgacag ttggcaaaag tgctgcacac gctacgccat gaggaatgtt gtaccatgca
    30781 cttaacgggt gagccatccc gtgtaccagt cctaaaccga catttgagaa gcccattccc
    30841 gcaatatact gtcctaatgc cattttttct cgcccatttt gatcgccatc tacagaagct
    30901 tccaaagaat ggccgatgat ttcgattgct tttaaatgca gcatatcagt catttcccaa
    30961 gcgccttttg tgataaaccc ttcaatagca tgcgtcatag catccatacc tgttgatgca
    31021 gccaattttt tcggcattcc catcatcata tcgctgtcga tgaacgcgac gattgggata
    31081 tcatgaggat caacacaaac aaattttcgg tgatttactt catctgtgat gacgtagttg
    31141 atcgttactt ctgctgctgt tccagaagtc gttggtatag ctaatatagg taaacatgga
    31201 tttttcgtat cagcaacacc ttctaaacta atgacgtctg aaaactctgg attcgtcaaa
    31261 ataatcccaa tggcttttgc cgtatcgatt ggcgaacctc caccgattgc gatcaaacag
    31321 tctgcctctg cctctttaac aaaagccact ccatctttta cattttggat cgttggatta
    31381 ggtacgatcc cgtcgtataa agcataagcg attgctgctt tatctagaag ttctgttact
    31441 tttgttgcta taccgtgctc gatcaatcct ttgtcagtaa tgacagctgc tttgccgaat
    31501 cctcgctgct gaaattcagg gacgatcgac tcaatcgctc cttttccaaa atacgatgtt
    31561 tcatttaaaa tcattcgatt actcattttc tattcctcct aatttgtatt atttttcaca
    31621 ataaaaacga aagaacccca agcaaccggt tgcccagttg cttgttgcta cagttcaaag
    31681 actggcatca attcccaact gactggagag gcatctggat ttgtctccat gatatcttcc
    31741 atgtacttcc accatttttg attgatagat gtatttgcca ttttttccca taacttttca
    31801 tcttcgatca ctaagtaacc aaataacaaa cttgtactcg gatcaagata gatcgaatag
    31861 tcgatgaccc cgtgttcacg aagtgctgta cgcatttctg gccaaagttc atcatgtctt
    31921 tttttgtatt cttcttgttt ttctgggtaa actttcatgc aaaaagcttt tttcaccatg
    31981 tcagacacct gctttcgtat gaacttcctc caaataacct gcttttggag tgacaccaaa
    32041 cgcatctgct aaatgccaca ggttttcgtc cgtgattgtt tgcaagattg gtccttgcgc
    32101 tttgacgtac gtatagactt ctgctgcctt ttctgctgtc tcgatcaaac caaagacttc
    32161 atccatgtct tttcctgacc cgtagattcc atgttgtggc cataaaacca atcgagtttc
    32221 tttcattttt tcagctgttg cttccccaat ctcatttgtt cccggaacca tccacggaat
    32281 gatggaaatt ccttctggaa aaacaactaa gcattccgta cacatttgcc atagcgtacg
    32341 actaaaggaa cgttcgtcta attcatgcgt aaaactcatc gctaatagat gactagcatg
    32401 gcaatgcata acgatccgat tatctgggtc tacttctaaa cgggcgatat gactcatgaa
    32461 atgacttggc aactcgcttg taggaaggca ttcgttttcc agcccccaca aaagttccag
    32521 tgttttgcca tcttcagcaa ctcgaacgat tccaagattc tcggcaggtt cgctcgctac
    32581 atttttaaaa tatcttcctg aaccagttac aataaaatag catcctgcta aacggctggc
    32641 atcaaaaatc atcgagatgc ggcggatcac tttttcagga tccaagaacg gtgtgatctc
    32701 ttcttctttt agtaaataag aaatattccc gccatttctt tcatcccatc ccaagcgata
    32761 aagattcgac gtcgtttcga tcatttcttt cacataaggc gcttgtaaaa tatctatttt
    32821 tttcaatata gccacactcc tattttctta attccaacac gtctttttga tattcctgga
    32881 cttcttccag ccaatccaaa ccaactggaa cttgattttt gtaacagaaa taattccata
    32941 catcggcata cgggaaatct ttcaattctt ccgtataagc cattcttgaa gtaaagtcaa
    33001 aagccagctc catttctttc aatcgctcaa ctggttccag catagctttc agcagtgctt
    33061 tttgtgtatt tctcgtaccg atgacccaag cagcgatacg gttgatcgtt gcatcaaaga
    33121 aatccagacc gatatttgtt ttttccaaca attcgttacg gactaattct ttagcgattt
    33181 cctgcaattc atcatccatc aaaaccacat ggtctgaatc ccaacgcact ggacgggaaa
    33241 catgcaacat gattcctttg ctgaataaag ctaatgaaga taatttattg gaaatcactt
    33301 ctgttggatg aaaatgtcca gcatctagac aaacaagttt gtcacgagtc aacccgtaac
    33361 ccatataaaa ttcatgggaa ccgactgtat aagcttcagc gcctaaacca aatagtttgc
    33421 tttctactgc atcttgtaca taccgttcat ctaactcttc tgtaaaaatt tcatcgagtg
    33481 attccattaa acgttcccgt ggggtaaaac gatcaatcgg attatctttc atcccatcag
    33541 gtacccaaaa gttattgata cttgtttgcc cagtttcttt cccaaagtac tccgcgattt
    33601 tgcggcttct tttcccgtgt tcgatccagt attcacgaac ttctttatct ggatgagcca
    33661 atgtaaagcc gtctttcatc attggatgcg aaaagaaagt cggattgaag tcaagaccga
    33721 tttcttcttt ttttgcccac tctacccatt tttcaaaatg tttcggctca atttcattta
    33781 gatcgacaga ttcttctgta tccaaataga tagcatgaag gttcaatttg tgtttgcccg
    33841 gtatcaacga ataagccttc tccagatctt gtctaagctc ttcaggtgtt cgtgcggctc
    33901 ctggaaaatt ccctgtcgcc atgattccac cggtcaattc gccatccggt gtcaagaaac
    33961 ctttgacatc atctccttgc cagcaatgca tagaaatttt gatgtcctgt atcttttcta
    34021 gtacagcatc tgtatctaca ccgatagttg catatttttc ttttgcttgt tcgtaatttc
    34081 tttcaatcgt gttcatacga ttactccttt gattttattc acgtaccatt ccatcgtgtt
    34141 ttgcttattt tcatctgggt aaaaagtttc acatggaaac gactgtttga ttacatttct
    34201 tgcttgttct atattcgaaa actgacctgt actgagcatt tgcatggcaa cattcccgat
    34261 tgctgtggct tcactaggtc ctgcttctac aatgattccg gcataatctg ctgtcaattg
    34321 attcagtaga cgattattcg atccgccccc tacgatgtgc agcttttcca atttcgttcc
    34381 agttaagtgt tctactttga tcagctcttg tgcgtaacaa agagcaagat tatcatacac
    34441 acaacgagcg atagctcccg atgagacagg gactggctgc tctgtttcaa aacagtattg
    34501 ctgaatctct tctatcatat ttgggggatt caaaaatcga gggtcattga tttccatcac
    34561 ttgctgcagc ggtttttctt ttgcagcaag ctctgccaat tccgcataac tgtactcgta
    34621 ctcttgcatt cgggcaactt cctggatcaa ccacatcccc atgatgtttt tcaaaaaacg
    34681 caccgtgtta tgggcacccc attcattcgt ataattttca gaaaaagcat tgcgagaaac
    34741 attggcgatc tttgtttcaa tccctaaaag cgaccatgtc ccactgctga tataacccca
    34801 acgttctcct tgacctggcg ttcctaatac agctgacgcc gtatcatgac ttgctactgt
    34861 gataaaggtc acatctggca aatcatattt tgaaaattgt tctttttgca gatctcctag
    34921 tatcgttccc ggctctgtca atggtggaaa taattccttc gagacaccga ttttttcaag
    34981 aagttctatt tcccattctt ttgtccctgc attcagcagc tgcatagtag atgcattagt
    35041 tttctccgtc accatgttac cagtaaagac ataccctaaa taatcaggta tcagtaacaa
    35101 tttatgtgct tttgccaaat tttctttttc ttcagcaaac aattggaaca aggtattgaa
    35161 aggctggatc tgaatccctg tctgctcata caacttatct agagaataga tttcagaaaa
    35221 ctgttctact gctccattgg tacgcttgtc ccgataagca atcggatcat ttatccgatc
    35281 cccttgttca tcaatcagac agtaatcgac tgcccatgta tcgatccccg caaaaaggcg
    35341 atcaatccct gtctgcttta atttttcaaa tccaatcaaa atctctttga taagataatc
    35401 gatcttccag cgatcgtgac cgtctttgtt ccgaaagccg tttttgaacc ggtgtacttc
    35461 ttccaagatc aaagaacctt ttgcatccct cgtacttttc attagtcgtc cgcttgatgc
    35521 accgatatcg atggcaacaa atgttggatt catttctcca cctcttttac tttattgttg
    35581 attagcactt tattaataaa gcgttatcat ttattgacaa tgctatcata atacagaatc
    35641 ttatattttg cttcacacta ttttggtata taattgcatt atcttgacaa gttgggagtg
    35701 tttacatgga ttactgggaa aaagccaacc actcatggtc ggaagactca aaaagagtga
    35761 tcttgacgcc tacacaaaga agcaggaatt tattttttta tatccaagaa atcggttact
    35821 tcaaagcacc aatcccttac tatactgaac gagcacattt accttcttac ttgataaagt
    35881 ttactttgtc tggcaaaggt tcgcttacat ataatgataa tcagcaacag ctaaaagctg
    35941 gagacttatt ttttattgac tgccaagaat atcagcgtta tcaaacgatc agcgaagaac
    36001 catgggaaat ggattggatc catttgaatg gtcagaacgt ccaatcattt tatcaggagt
    36061 tcacaaaaga cggccataac accttccatt caacaggaga tccatcagaa aacaagatcc
    36121 accatttgat cacagaattg atccacatgc aacaaggcac gaatgcccgt accgatttcc
    36181 agtcctctgt agctatccac gagttgctga atgaactgat tttgcaaaag tatcagctag
    36241 atttcgaaga agaggatatt cccgcctacg tttacaaact gaagcaatac ttagacgaac
    36301 atttcaaaga aacgattaca cttgatcaat tagaacaaca attcttattg aataaatatc
    36361 agctgagcaa agatttttcc aaatttatcg gttctccacc aatcgattat ttgatcagca
    36421 aaaaactttc ttacgctaaa gaccttcttc ggtatacaga atttacggtt cgaatgattt
    36481 ccgaagaaat agggattgag aattttgctt actttagccg cctgttcaaa gcaaaaacag
    36541 gactatcgcc gagagacttt cgtaaattca gctgattcat ataatatgta cgtcattttt
    36601 tcaaaatatt ttgaaatata tagataaaaa taaaataacc gcagtttctg cttctatgta
    36661 aacaacagca gctactgcgg ttatttttta gtgatcattc tcattgttca tcttcaaaat
    36721 gttcataatg aaccaactgt ttctgtgtat cacgtttcaa aaattcttta ggcgttatgc
    36781 cgaactctct cttgaatgct ttcagaaaat gcggataaga agaaaaacca cttttactat
    36841 atacagcact agagctgcgg tattctcgca acaagtgctt ggcatagatc agctttttct
    36901 tcattacata ctgatgaaac gtaaatccag tgtacttttt aaattctcgt gtgatatggt
    36961 atttgctgac aaaaaactgc ttttccattt cctctaaaga aagcggatga gataaatttt
    37021 ttgagatata ttgtaacatt tgttccacac gctcattagg aactacagca ttctgttctt
    37081 gcgcactctc ttctttttgt acaacttgat tgagaaaaat caagtattct accagagctt
    37141 gttcatattt tatgtcttca ccgtaaattt ttttatcagg aggatggtcg ataaatgata
    37201 atttttgctt caatacttcc tgttctattt gcaatacacg actcctcttc tctcccattg
    37261 gttgaaaaca agccgataga ttagaccatg gcgtggaacg actttctaga aaagaagggg
    37321 tgacaaagat atagacacgc tcgaatacat ctgaagattg gcggacgata cgatgcaaat
    37381 cattggaatg gatgagcagc acacttcctg ctttgatcgt aaattgtcgt ccgttcaaat
    37441 aaaaagtcgc ttctcccttt aatgtagcat ggatctcata gaaatcatga tgatgataga
    37501 tcgttttatt ttggctgacg acatctttga catgatagat ttcatactga tcagaaacca
    37561 tcgtattgac ttgttcaccg ctgattttct tttggagcat gtgccgcacc tccttttgtt
    37621 cacttatgca caattttatt ataccacgtt ttttgtaatc aaaaagagct gcttttatat
    37681 actcttataa aaaagaagag acgtggcatc ttctagcccc gccccctcca ttctaaatat
    37741 tagttaacca acgtcttctt tacattattc aagaagactt cttccataac gaatcccatc
    37801 atcttttgtt tcttcaagaa ccacatacag gtacggacga ttcttcatga tgatttcttt
    37861 gatttttgtg tactcgatca caccgtctcc caaatttacc ggcacgattt tttcctgttc
    37921 gacacgaaaa tctttcaaat gaaccgcaca tatcttttca ccaaaacgtt caaaagcttc
    37981 ttctaccaat tggacttgtt cttgataatc actacttgtg atcaaattgg tcggatctaa
    38041 aatgattcct agatagtccg aatctactgc ttggatcagc tgctctaccc ttgctaaaga
    38101 atacaccgga tgattcaaac ctggttctat ccccaccatc atttggtatt tttctccagc
    38161 ttttaccaag cgacggataa cggacacagc ttcagcaaat gcttcatctg tgaaattttc
    38221 ttccgtatat tggatttccg gcaaaacaca tccagtttcc gaagcaacca tcgacgcacc
    38281 aaaggaagaa gcatagcgaa gatactgctc gaactttgtc agcaactctt gtcgtatcgt
    38341 taaatctgga tgaatcatat tgatatagca acttaaaatt cctacggtca cttcttgctt
    38401 ttctaaaact cgtttgacat aatttcccat cccagaattg atctcatttg cgccagaagg
    38461 tatttcagga aacgaaagac ctaaggcgag ctgcagtgta tgaatccctt gttcttttgt
    38521 tttttctgca agatcttcta tcgtttggac ttcggaaaga tcatgtcctc taattgtaat
    38581 gttcaacata actattctcc taactcagca tattgggtag ccacatgacg atttgcggga
    38641 agtaagtaat caaaaacaag acgactaaga taaccccata aaaaggaaga agcggtttca
    38701 gcattgcctc tgcttttacg cctcctacac ttgcaccgac atatagtcct gtacctactg
    38761 gcggcgtgat ggagcctaca cacagattca tgattgaaaa caacccgtaa tgtacaggat
    38821 caacccctac acttgtgacg attggtaaaa atattggtgt aaaaatcaag atagcaggcg
    38881 ccacatccat aaacataccc accagtaata acacaagatt gactaataac agaataataa
    38941 atttattatc cgttacgccc ataatcaacg aactaattgc ctgaggaata ccggtaaacg
    39001 ccatggcgaa agacatcatg gaagaagtag ccaataagaa catgatcgtc cctgacattg
    39061 ctactgattg aaacaacata ttaggtatat cttttagttt taccgtttta tagtaaaaca
    39121 tagcaatcaa cagggagtaa atgacagcta tcgctgaagc ttctactgct gtaaatatcc
    39181 ccgtcaagat accaccgata atgatgacaa tcaatagaag gctaggaatc gcctctttta
    39241 ttatttttcc agcatgagcc atctgtcctt tttcgacaac agggtattta ttctttttag
    39301 caacgatgaa tgctactagc atgattccta gcgcccacaa agcgcctacg agataacctc
    39361 ccataaacaa agcagagatc gaagcaccac cagcaacaag tgaatacatg ataaaagcag
    39421 tacttggagg aatgaccata cctgtcggag ctgacgcgat attgaccgct gtcgcaaact
    39481 tccggtcata tccttcttcc acttgctgag ggatcaacac tccgccaatc gcggtagaag
    39541 cggcaatggc cgagctagag atggaaccaa ataaagcatt ccctaacaca ttcgtatgag
    39601 ccaaagcccc cggcacgcgc ccgacgaaca acatcgcaaa atccactagt ttcttagcta
    39661 tccctccatt attcatgata atgcccgaga gaacgaaaaa cggaatagcg atcagcgaaa
    39721 aactattgat acttcccacc attttttgag ctgaactaaa agcagaaaca tcaaacggta
    39781 taactaacaa taaagtcatc atcgatgaga atgcaatact tatagcaatc ggcatcccta
    39841 atacaagcaa cacaacaaat acaaaaaata acactaatcc ggcttctatt gccattttat
    39901 tcctccattt ttatattttt tcttgaattg ctggcttgtt gcttaatgta tcgattagat
    39961 tgatcaggct atagataatg atgagtaccc ccgatacagg tactgacaga taaacatatc
    40021 ccataggaat acttaaagaa ggcgagatct gccccattgt caatgtgaca gcattccatc
    40081 cgccatacac catgatcatc gcagcaaata ccagaaataa actttgaaca acaacttcta
    40141 aaattttact ttttttgcca gttaattttt cacttaaaac cgttacggct aaatgtgctt
    40201 tttgtcccac aacatacgcg gaagacaaca tggcaaacca gacaagaccg aatcgaacaa
    40261 gctcttccgt gaccgtattt ggatttccta aaaccgttct ggaaaatact tgccataaaa
    40321 caacagcgac catcccaaca agcaaaacag ctcccaggat ctctaatacc ttgtttaaaa
    40381 tcattttcat cacagctacg ctccttgctc ttggatcttt tgataaaggt ctttgtaggt
    40441 aggatcgttt ttgaatatat catgaagtgg ggcaactgcc tctcgaaacg gtgtattatc
    40501 tacatcattg aattggactc catgttcttc ttgtgctaaa gctttttctt cttcaattgc
    40561 ttttgcaaaa gcttcaattt caaaaaccgt tgattctttc gctgcttctt caatggcttt
    40621 ttcctgctca tcagttagct gttctttgat ggcatcattc atgatgatga tatctggcac
    40681 acgtgtatgc tcatcatagg aatagtattt agccacacca ccatgtccag ctgtatacaa
    40741 gacaaattca ttgttttctg atccgttgat caaattggat tgaagagacg tgtacacttc
    40801 atcacttccc ataggtacgg gcgaaccgcc taaaagctct accattttga tagcggtttc
    40861 gctttgcatc acacggatct tctttccctt caaatcttcc ggcgtatgga tcggaccgtc
    40921 taccatataa aagctgcgct gaccagaatc ataataggtc aatccggtaa atcctaaatt
    40981 tttggtcgat tggtagattg gctccattat ttcttcatta tccatcacct taaagaaatg
    41041 ttcttcatca ttgaatagat aaggaactga aaaaatcgaa taatctttag agaaactttc
    41101 caacgctgag gcacttactt ttgtgaaatc tactgctcct gactgtgtca attcgatcag
    41161 ttcacgttct ccacccagtt gtccatctgg gaaatactgg atctgtacgg aaccatctgt
    41221 tttttctttt actaaacgcc caaattcatt cattgcatca attactggct ggctgttgct
    41281 tgcataagca aagcgcaagg taactatctc atttacatct gcatctgcga cagttgccac
    41341 actagaatta tttccgcagc cgcttagacc tagcagacct aggctaagcg aacttgctgc
    41401 aaacagcagt gatgtttttt tcatatgggt ttcctttctt ttaacaataa gagacgagca
    41461 attctgtcaa acaaagcacc gttaacgact gtccataagg cattgctgtg attccgatat
    41521 tccgataaaa atctaaactg tcccccattc ctgtcccgat tgaaacgttt tgaacttctc
    41581 ctttttcatc gatttgttca agcaatcctt ggatagcacg ataagcgact tctttatatt
    41641 cttgtggtaa ataacgttta tggaccgctt tcaaaatacc gtaagcaaat cctgcagttg
    41701 cagaagattc tacgtaagaa gaaggatcat ccaacaacgt atgccataaa ccgctttcag
    41761 actgataagt ttttaatgca cgcacttgtg cttcaagtgt ttcgatcaat aatttgcgca
    41821 atgcatcttc tttcgataat tctaagattt caatgatctc tggaatagca atcgtcaacc
    41881 agcagtttcc acgagcccaa aatgcttcgg catagttatg atttccttca aacgtccagc
    41941 cgtgatacca caatcctgtt cgtttatcca tcaagtattt gatatggatc ataaactggt
    42001 aacgagcttc ttctaaatat tctgggcgat tcaacagttt cccgattttt gccaaaggaa
    42061 gcactgtcat catcaatgta tcgtcccaca actgattctt attttccggt ccatatgtgg
    42121 catgctgtaa cccgtcttca tttgtccgag gcatgtcatt catgacccat tctgcccatt
    42181 gttctaaata aggcaaatag cgtgaatctt tcgtgtcttc atacaagtaa gccatagtca
    42241 atagcggtgc cattgtattg acgtttttgg gcggagcgcc ttcttccata cgatcctcaa
    42301 accattcatt gaccacttga taagcgcgag gattttttgt catctggtaa tttttataaa
    42361 taccatatag accgacacct tgtggccaat tccacactgt ccagcttttg tcatctactt
    42421 tcagtccatc aaagtccaac aaaaattcac cattatcgtc tttgatcgtt gtcaggtttt
    42481 caacgagcag gtcaattttt tctttcacta attctttagg tacaaaaatc tcttctttaa
    42541 taatcatctt tacgccacct ttttaaacgc tttcatttta tatccttaat atagcagtaa
    42601 taccgtccac tataaatatc gctttttgaa caaaaactgt ctaaaattga cctaaatgtt
    42661 aaataacaaa agtaactttc agttacttgg aaattaaaaa aagaacttgg agaaacaacg
    42721 ttttccaagt tctttttcat ctattttctt atcttttttc tacgctttct tttgcttatc
    42781 cgttgtaagc atcaatgatg atctgtttca attcagagat caatggttct ttcggattag
    42841 ctgtcgtaca ttggtcttcg taagctaatt cagccatacg gtctacggtt gtatctagtg
    42901 tttcttgtgt gactccttga gcttttagac tcatgttgat gccgacagat tttcctaatt
    42961 cataaacagc cgttgctaaa gcttcgacta attcctctgt tgtttcgcct ttcagaccta
    43021 agaattttgc gatatctgcg taatctgtat ctgcacggaa atagtcatat ttagggaaca
    43081 tcgcatgttt ttgtggatct tttgcattat agcggataat atgcggtaaa agaatcgcat
    43141 ttgtccgtcc atgagggatc ccgtattccc cgccgatttt atgtgcgact gagtgacaaa
    43201 tacctaggaa tgcgttagca aacgccatac cagccattgt agaagcgttg tgcatttttt
    43261 cacgtgattc catatcaggt gtttttactg atttttctaa atagtcaaag actaacttga
    43321 ttgcttgtag gctcaatcca cgtgtataat ctgaagccat gactgaaaca taggattcga
    43381 ttgcatgtgt taacacatcc atcccggtat ctgctgtgac agatgctgga actgacatga
    43441 cgaattgagg atcgacgatt gcgacatctg gcgtcaatgc ataatctgct aacggatatt
    43501 tcacatgtgt ttcactgtca gtgatgaccg caaatggcgt tacttcagaa ccagtccctg
    43561 atgttgtcgg gatacatacg aattgtgttt tttctggttt gtcgatctta tatgtccgtt
    43621 tacggatatc caagaatttt tgttttgcac caaagaaaga tgtgtctggg tgttcataga
    43681 acatccacat tcccttagct gcgtccattg cagaaccgcc tccaagagcg attactgtat
    43741 ctggcttgaa gtctgccatc agtttcgttc ctgcgtatac ggtatttgtc gatgggttcg
    43801 gttcaacatc tgagaagact tcgatcttca catcattttt acgtcgtgcc aatacttcac
    43861 gaactgtatc gcagtaaccg aattggacca taccagggtc acaaacgatc atgacacgtt
    43921 caacgttttc cattttctct aagtacaata gagagttctt ttcaaagaag atttttggcg
    43981 gtaatttgaa ccattgcatg ttatttctcc gtttcgctac agttttgaca ttgattaagt
    44041 ttacagcaga gacgttacga gaaacagagt tcttaccata agaaccgcat cccaaagtca
    44101 atgacggaat catttcgtta tagatatcac cgatgccgcc ttctgcagat ggtgtattca
    44161 ctaaaatacg acaagctttc attcgaagac cgaatttgac atgcaactct tcgttttctg
    44221 aatggataac tgctgtatgt cctaatcctc caagattcaa cataccttca cacaaatcaa
    44281 acgcatggtc cgtgttgtta gctttgatca tggcaagaac tggagaaagt ttttctcttg
    44341 atagcggata atctgggcct acgccttcca attcagcaat cagcattttc gttccttttg
    44401 gtacactgat tcctgccaat tttgcgattt ccatcgcaga atgtccaacg attgatggat
    44461 tgaccgcata ttttccttcg ttcatcactg catcttctaa tttttgcaat tcatctggtt
    44521 ttacgatata aacttggtgt gcttggaatt ctgcttttac tgctgcatag atttctttat
    44581 ccacgatcac agcttgttca gaagcacaaa tcattccatt atcaaatgtt ttcgaaacga
    44641 tcaaatcgtt cactgcacgt ttaatttttg ctgttttttc aatataagct ggtacgttac
    44701 ctggacctac acctaaagct ggttttcctg tagagtaagc agacttgacc attccagctc
    44761 cacctgtcgc taagacgatc gcgatacctg gatggttcat tagcatagaa gtcgcttcga
    44821 tcgaaggatg ttcgatccat tggatacaat tttcaggagc tccggcagcg atagcagcat
    44881 cacgtaccac tcgtgctgct tctgcagaag atttttgggc acttggatgg aaagcaaaaa
    44941 cgattggatt tcttgttttc aacgcgatca ttgatttgaa aatcgttgtg gaagtcggat
    45001 tcgttgttgg agttactccg caaaccacac cgactggttc agcaatttca atcaaccctt
    45061 tttggacatc ttcattgatc acaccaactg ttttatcatg tttgatatta tgccaaatat
    45121 attcggacgc atacatattt ttgattgctt tatcttcgta aacaccgcgg cctgtttcct
    45181 ctacagccat cttcgctaat ggcatatgtt gatccaaagc tgccattgcc atttcatgca
    45241 cgatatggtc tactttttct tgatcaaagc tttccatttc tttcaatgct gcatttgctt
    45301 tttctgccaa atcatcaatc attgcttgta cgtcaattgt cttcgttttt tctttttcta
    45361 ttactttagc catcattatc ctcctaatgg gtcatggtta tttgttaaat aattcacatg
    45421 tatatcatac aatatttatt tttctatgta aacacttttt tgtgattttt ttcacag
//
LOCUS       DAZKNH010000013        44847 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000013 DAZKNH010000000
VERSION     DAZKNH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.013"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG8MG_000666"
     CDS             212..604
                     /locus_tag="ACG8MG_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0374734.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG8MG_000667"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG8MG_000667"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFQ0374735.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG8MG_000668"
     CDS             2698..2943
                     /locus_tag="ACG8MG_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374736.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG8MG_000669"
     CDS             3165..4775
                     /locus_tag="ACG8MG_000669"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFQ0374737.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG8MG_000670"
     CDS             complement(4752..4964)
                     /locus_tag="ACG8MG_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374738.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG8MG_000671"
     CDS             5067..5936
                     /locus_tag="ACG8MG_000671"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFQ0374739.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG8MG_000672"
     CDS             6101..7372
                     /locus_tag="ACG8MG_000672"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0374740.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG8MG_000673"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG8MG_000673"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFQ0374741.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG8MG_000674"
     CDS             8763..9026
                     /locus_tag="ACG8MG_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFQ0374742.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG8MG_000675"
     CDS             9471..9920
                     /locus_tag="ACG8MG_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0374743.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG8MG_000676"
     CDS             9977..11395
                     /locus_tag="ACG8MG_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0374744.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG8MG_000677"
     CDS             11395..12255
                     /locus_tag="ACG8MG_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0374745.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG8MG_000678"
     CDS             12429..13202
                     /locus_tag="ACG8MG_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0374746.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG8MG_000679"
     CDS             13349..13714
                     /locus_tag="ACG8MG_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFQ0374747.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG8MG_000680"
     CDS             complement(13862..15070)
                     /locus_tag="ACG8MG_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0374748.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG8MG_000681"
     CDS             complement(15089..15367)
                     /locus_tag="ACG8MG_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374749.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG8MG_000682"
     CDS             15560..16216
                     /locus_tag="ACG8MG_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0374750.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG8MG_000683"
     CDS             complement(16658..17455)
                     /locus_tag="ACG8MG_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFQ0374751.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG8MG_000684"
     CDS             complement(17452..18636)
                     /locus_tag="ACG8MG_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFQ0374752.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG8MG_000685"
     CDS             complement(18661..19908)
                     /locus_tag="ACG8MG_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0374753.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8MG_000686"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8MG_000686"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFQ0374754.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG8MG_000687"
     CDS             21888..22202
                     /locus_tag="ACG8MG_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFQ0374755.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG8MG_000688"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG8MG_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFQ0374756.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8MG_000689"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8MG_000689"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFQ0374757.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG8MG_000690"
     CDS             23546..23875
                     /locus_tag="ACG8MG_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFQ0374758.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG8MG_000691"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG8MG_000691"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFQ0374759.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG8MG_000692"
     CDS             24863..25687
                     /locus_tag="ACG8MG_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFQ0374760.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG8MG_000693"
     CDS             25680..26027
                     /locus_tag="ACG8MG_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFQ0374761.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8MG_000694"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8MG_000694"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFQ0374762.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8MG_000695"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8MG_000695"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFQ0374763.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8MG_000696"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8MG_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFQ0374764.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8MG_000697"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8MG_000697"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFQ0374765.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACG8MG_000698"
     CDS             31684..32451
                     /locus_tag="ACG8MG_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0374766.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFIVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACG8MG_000699"
     CDS             32441..34483
                     /locus_tag="ACG8MG_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0374767.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="ACG8MG_000700"
     CDS             34764..34913
                     /locus_tag="ACG8MG_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFQ0374768.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8MG_000701"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8MG_000701"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFQ0374769.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8MG_000702"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8MG_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFQ0374770.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8MG_000703"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8MG_000703"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFQ0374771.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8MG_000704"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8MG_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFQ0374772.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="ACG8MG_000705"
     CDS             39330..40412
                     /locus_tag="ACG8MG_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFQ0374773.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="ACG8MG_000706"
     CDS             40412..41215
                     /locus_tag="ACG8MG_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0374774.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="ACG8MG_000707"
     CDS             41285..42025
                     /locus_tag="ACG8MG_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0374775.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="ACG8MG_000708"
     CDS             42036..43040
                     /locus_tag="ACG8MG_000708"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFQ0374776.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..>44847)
                     /locus_tag="ACG8MG_000709"
     CDS             complement(43085..>44847)
                     /locus_tag="ACG8MG_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374777.1"
                     /translation="LFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDSK
                     YTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESN
                     PGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVL
                     TMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPF
                     VHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELP
                     ELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIY
                     QTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHI
                     QEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLIL
                     ARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIY
                     LSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQE
                     KDTFIDRITVQLSRNYPNPV"
BASE COUNT        14414 a         7826 c         9340 g        13267 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc atagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataaca
//
LOCUS       DAZKNH010000014        41658 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000014 DAZKNH010000000
VERSION     DAZKNH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.014"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..858
                     /locus_tag="ACG8MG_000710"
     CDS             34..858
                     /locus_tag="ACG8MG_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0374778.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            868..1116
                     /locus_tag="ACG8MG_000711"
     CDS             868..1116
                     /locus_tag="ACG8MG_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374779.1"
                     /translation="MKIIRILVFNLSFLAATILCCGLIFSQLSIYPRYQRLFIHFLNI
                     QCASMPLEVIKKLLINPIFMVSLVLLTGLVTWVLGKKE"
     gene            1159..3258
                     /locus_tag="ACG8MG_000712"
     CDS             1159..3258
                     /locus_tag="ACG8MG_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFQ0374780.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(3296..5353)
                     /locus_tag="ACG8MG_000713"
     CDS             complement(3296..5353)
                     /locus_tag="ACG8MG_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFQ0374781.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(5467..5679)
                     /locus_tag="ACG8MG_000714"
     CDS             complement(5467..5679)
                     /locus_tag="ACG8MG_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374782.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            5866..6333
                     /locus_tag="ACG8MG_000715"
     CDS             5866..6333
                     /locus_tag="ACG8MG_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374783.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            6330..6572
                     /locus_tag="ACG8MG_000716"
     CDS             6330..6572
                     /locus_tag="ACG8MG_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFQ0374784.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            6590..6892
                     /locus_tag="ACG8MG_000717"
     CDS             6590..6892
                     /locus_tag="ACG8MG_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374785.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            6964..9018
                     /locus_tag="ACG8MG_000718"
     CDS             6964..9018
                     /locus_tag="ACG8MG_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374786.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            9018..11630
                     /locus_tag="ACG8MG_000719"
     CDS             9018..11630
                     /locus_tag="ACG8MG_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFQ0374787.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            11627..14158
                     /locus_tag="ACG8MG_000720"
     CDS             11627..14158
                     /locus_tag="ACG8MG_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFQ0374788.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            14210..14542
                     /locus_tag="ACG8MG_000721"
     CDS             14210..14542
                     /locus_tag="ACG8MG_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374789.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            14543..15136
                     /locus_tag="ACG8MG_000722"
     CDS             14543..15136
                     /locus_tag="ACG8MG_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374790.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            15299..16993
                     /locus_tag="ACG8MG_000723"
     CDS             15299..16993
                     /locus_tag="ACG8MG_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFQ0374791.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            17681..19558
                     /gene="ltrA"
                     /locus_tag="ACG8MG_000724"
     CDS             17681..19558
                     /gene="ltrA"
                     /locus_tag="ACG8MG_000724"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0374792.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            19689..20066
                     /locus_tag="ACG8MG_000725"
                     /pseudo
     CDS             19689..20066
                     /locus_tag="ACG8MG_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            20066..21271
                     /locus_tag="ACG8MG_000726"
     CDS             20066..21271
                     /locus_tag="ACG8MG_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0374793.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            21287..21931
                     /locus_tag="ACG8MG_000727"
     CDS             21287..21931
                     /locus_tag="ACG8MG_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374794.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            21942..22748
                     /locus_tag="ACG8MG_000728"
     CDS             21942..22748
                     /locus_tag="ACG8MG_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374795.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            22770..23000
                     /locus_tag="ACG8MG_000729"
     CDS             22770..23000
                     /locus_tag="ACG8MG_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374796.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            23546..23821
                     /locus_tag="ACG8MG_000730"
     CDS             23546..23821
                     /locus_tag="ACG8MG_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374797.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            23863..24285
                     /locus_tag="ACG8MG_000731"
     CDS             23863..24285
                     /locus_tag="ACG8MG_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374798.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            24304..25896
                     /gene="mobP2"
                     /locus_tag="ACG8MG_000732"
     CDS             24304..25896
                     /gene="mobP2"
                     /locus_tag="ACG8MG_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFQ0374799.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            25908..26243
                     /locus_tag="ACG8MG_000733"
     CDS             25908..26243
                     /locus_tag="ACG8MG_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374800.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            26390..26584
                     /locus_tag="ACG8MG_000734"
     CDS             26390..26584
                     /locus_tag="ACG8MG_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374801.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            26616..28988
                     /locus_tag="ACG8MG_000735"
     CDS             26616..28988
                     /locus_tag="ACG8MG_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFQ0374802.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            29049..30509
                     /locus_tag="ACG8MG_000736"
     CDS             29049..30509
                     /locus_tag="ACG8MG_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFQ0374803.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            30520..32724
                     /gene="topB"
                     /locus_tag="ACG8MG_000737"
     CDS             30520..32724
                     /gene="topB"
                     /locus_tag="ACG8MG_000737"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0374804.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            32740..32889
                     /locus_tag="ACG8MG_000738"
     CDS             32740..32889
                     /locus_tag="ACG8MG_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374805.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            32963..33556
                     /locus_tag="ACG8MG_000739"
     CDS             32963..33556
                     /locus_tag="ACG8MG_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFQ0374806.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            33572..34471
                     /locus_tag="ACG8MG_000740"
     CDS             33572..34471
                     /locus_tag="ACG8MG_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFQ0374807.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            34474..34959
                     /gene="ssb"
                     /locus_tag="ACG8MG_000741"
     CDS             34474..34959
                     /gene="ssb"
                     /locus_tag="ACG8MG_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0374808.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            35325..35585
                     /locus_tag="ACG8MG_000742"
     CDS             35325..35585
                     /locus_tag="ACG8MG_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374809.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            36026..36232
                     /locus_tag="ACG8MG_000743"
     CDS             36026..36232
                     /locus_tag="ACG8MG_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFQ0374810.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            36232..36483
                     /locus_tag="ACG8MG_000744"
     CDS             36232..36483
                     /locus_tag="ACG8MG_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374811.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            36498..36935
                     /locus_tag="ACG8MG_000745"
     CDS             36498..36935
                     /locus_tag="ACG8MG_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374812.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            36928..37635
                     /locus_tag="ACG8MG_000746"
     CDS             36928..37635
                     /locus_tag="ACG8MG_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374813.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            37800..38000
                     /locus_tag="ACG8MG_000747"
     CDS             37800..38000
                     /locus_tag="ACG8MG_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374814.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            38016..38195
                     /locus_tag="ACG8MG_000748"
     CDS             38016..38195
                     /locus_tag="ACG8MG_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374815.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            38536..38799
                     /locus_tag="ACG8MG_000749"
     CDS             38536..38799
                     /locus_tag="ACG8MG_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFQ0374816.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            38793..39143
                     /locus_tag="ACG8MG_000750"
     CDS             38793..39143
                     /locus_tag="ACG8MG_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0374817.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(39167..39781)
                     /locus_tag="ACG8MG_000751"
     CDS             complement(39167..39781)
                     /locus_tag="ACG8MG_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0374818.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            40095..40517
                     /locus_tag="ACG8MG_000752"
     CDS             40095..40517
                     /locus_tag="ACG8MG_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374819.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            40527..40730
                     /locus_tag="ACG8MG_000753"
     CDS             40527..40730
                     /locus_tag="ACG8MG_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374820.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            40941..41525
                     /locus_tag="ACG8MG_000754"
     CDS             40941..41525
                     /locus_tag="ACG8MG_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0374821.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT        15267 a         6605 c         8400 g        11386 t
ORIGIN      
        1 ttttatttat aaaaaaataa ggaggagatc ggaatgaata agagaaaatg gctgtttgtt
       61 tgtgtagcag ctctcttttc tactgtagct tattcgcaac tgtctttcgc tgacagcagt
      121 gatagtagta ccactcaaac aatagataat tcaattactg caccaagcga aacggacagt
      181 tcggagaata ctacagaaga aacggcagat tcaaatataa gctttactga atcaacgaat
      241 ccaacgtcag aaagttcttc agagacttca gaaaagccgc aagacgtaga agatacgcaa
      301 gtgatcaagc aagccattca tattcaaaaa attatttctt cgaatcaaat caacaatcaa
      361 ggcagtcaag tagcccagca agaaggtatc aatggcgcga agtttattgt ctacgatatc
      421 acgaatatct tagaagaaat gtcacaagat gataatgaga aaaatcaatc tgtagaaata
      481 attgagtcaa aattgaaaga tcgagcaaaa aaactttcat acgaccagtt gaaaaaagtg
      541 gctgaaggag aaaccaaaac gattgatgat caggaaggcg tgattgaatt ttccattgaa
      601 gtacctgcaa acaaaaaaca agcatactat atcgttaatg aatcaagccc tgaaaacatt
      661 tcaaacagtg aagatattgt tttattgaca cctgtttcaa atgaaaaagg tgaatttttg
      721 acggatgttt ggttataccc taaatcagag aaatcagagc cgaaagaaga aattaaaaaa
      781 gttgtgtcaa ctggtgtaaa gaagaacttt tttgaaaatt gttgggaatt ttttgctcaa
      841 ttattttttg ataattagga tgaaatgatg aaaataataa gaatattagt ttttaatcta
      901 tcctttttag ccgcaacaat attgtgttgc ggtttaattt tttctcaatt atcaatttat
      961 ccaagatatc aacgattgtt cattcatttt ctaaacattc aatgtgcttc catgccatta
     1021 gaggtgatta aaaagctctt gatcaatcca atttttatgg ttagtttggt tcttcttaca
     1081 ggattggtaa cgtgggtatt aggaaaaaaa gaatgaaaag cttacgatcg tttaaaaaaa
     1141 tattagatgg agggcattat ggaaaataaa attcattgca ctacgggaag agtaattact
     1201 ttagtgttgt ttttgttcct tcttcttttt gcgaggggaa ctgcagtcca ggccttagaa
     1261 aaagttgatc cgagtcaaat caaagaaaat aataatctgg tgctttatca tgatagtgat
     1321 tcattgagaa atatcgaagt aactgggatc aatggattta agatactaga tgagtattca
     1381 actaaaaaac aaaaagtact tgcttggcca aaagtcaaag atttgaaaag tacgtcaaaa
     1441 atcgttgccc attatcaaaa agtaggtact tatctaggaa aagaaattga ttgtgacgtg
     1501 actttttcta attttgtagc gcacgctggg gttaaccaag acggattagc taaaacaatt
     1561 aatggcaaaa agtattataa ttatttgctc tttgataaaa atatttatcg aggatttttc
     1621 atgcagtcaa tcaatgtcat gaatgcaaaa ctagaattta agtatgcagg aacaaaaaca
     1681 aaagtagctt tgagtgaacc acagccagaa attcaatcaa aaatgagatc gatgagagca
     1741 gctggtattg atttaaacga tacatttcta tccatttaca gtttaaatgg acagtatcag
     1801 caacgtgtgt cttggtataa tgacaataat gaatctgtcg gtgaacgtaa tattgatatg
     1861 agagaatttg ttgggtatga aaaaatgggt agcttacctt attttgtcac aacagataca
     1921 gcatgtgcag aatacaaagc tcctgcgtta tcaacaaaca atttaacttc aaaagtagtg
     1981 ggaggacgtg cagaaaaggc ttatagctcg aatgatcatt tcaccgatgt tgtaggagct
     2041 gatacttatc acagaagtgg tgtaacgtat acgcttcaag gcgcttcccc aacattcatg
     2101 attggcgcaa atacgaatag tatgatgttt agctttgata ctgcattgct atggacacca
     2161 caaccatcga agcctacaaa agaagtgttt aacaaagcta atactgaaga ggcagcacac
     2221 aatattgaca aaaaagtgat tccacaagga tcagatgttt actatcatat tcaccaaaag
     2281 tttgatgcat taacagtcaa cacaatgaac aaatacaaat catttaaaat cactgatacc
     2341 tttgacagca aaaattttga tatggtatcg gatgggaaaa actatgatgg cgcattgtgg
     2401 tatgttgaag cgaatggtac ttggaaaaaa gctgatacag gaggaaagtt aactgtatca
     2461 ggtaatacag tcacttatac tgcttctgac agctttttag ctaacgctgc aaattacggt
     2521 agggattata ttttgcagct ccatatgaga gcaaaaaatg atgcaaaagg aattgcacca
     2581 aatgtggcta ctgtaacgtt taacaatgat ccgaacaaag gaacacaaaa aacaaataca
     2641 gtagaaaact atttctctct taaaccaaat aaaaaagtgg aacaagatgg gaaagatgtt
     2701 gatggtcgaa atgatggtaa aaaaggaacg cctacagctc ctttaaatgc aggatctgaa
     2761 gtacaatatc tagtcacaca aaaatggcat acaaaaggtg tagacattgc ctcagatcat
     2821 tacaagcaat ttagtattca agatccagtg gaagatcgtt tgacgtataa agaaggttct
     2881 gcgcgagtga tagatatgag tacaggaaaa gatattactt cagaaggtac actaacctat
     2941 gatagcaatt caagaacgct gaagtgggaa gcttccgctg atttcttaag taaaaatctt
     3001 ttagatggac gagaaattca gctgatattt acagctaaaa ctcctttgaa tgaagaattg
     3061 gatatcgaca accaagcaaa agtttcagta gaaagtattg agaaagcaac taatgtagta
     3121 acgattgggg taaaacctaa cttacctcag atcattgtgc caaaaactgg atcgaatcat
     3181 ttagtgctaa ctgttattgg aagtatatta tttcttactt tagcatcatt gggctatgta
     3241 gttttaaaac tcaaataaat ataaaaacaa ggctgagaat cagccttgtt ttcttttatt
     3301 tgaagattgg attcgcacta ataaatacat tatctacgcc aattctacca gcagagtcaa
     3361 ctacaaaatt atgacctaat ccgagaatac tttggttatt ataaagtaaa tttgcattat
     3421 gggcagggct atttttccat cctaccatgg catcgatagc accatcgtct gataaaaggt
     3481 catcaatata cagcccagtt tgacttatgt tttctccctt agcaccgtag cagcctaagc
     3541 tttccaataa atcttggaaa gtaccaccac cagggcgata gtgactgaag ctgacttgta
     3601 attctttagc tcgaatatct gcagcctgtt gaacaatatc attgaattgc attggggcaa
     3661 caccattttg ttggcgataa tcatttaacc aagcaagttc tttttgacgc ataaagttac
     3721 gtaattcgtc taatgaaatc tttttatatt tgaatgtgat tgttttattt tgatcgacgt
     3781 tattaatgga tcgttggccg tttttatcca cggtaacggt aggatcgtca tttctgcaca
     3841 agaaataatt ttgatcagtc atgtttttcc aaggagtagc aaagctatcc ccttcgtata
     3901 ctgtttttga ttcactagag agaacagcgt tggtatcagc atttacatga ttaacagtca
     3961 cattaaattt acgagtgtag ttgaaaatat aattttgact ttcagtcaca ttgtttaagg
     4021 ttactgaatt tgttggttct acccgatcgt ctaaaacata tccttgaata gaaggagcag
     4081 ttaaagtgac actgtcaccg tgagtgacat tgactatctg ttctttcaaa atattaactc
     4141 gtccttcgtt tttgtagttg ttattgcaca acaaattgat ttgatgcact tgattataat
     4201 tgaatgtgat tgttgtatta cctgtgattg ttttgctttg agtatcatta ccaatcactt
     4261 tgtagctaaa attatcttta gcgccttcgc tatatttaaa tgcttttgcc ttagcagtgt
     4321 aagtagaacc agaagttact tttgcttgat ctgtagcaat cacttgacct gtttgattgt
     4381 caatatggtt aatagtaacc gtatattctt ttggtgtttc aggtttgtgt tgcaaccctt
     4441 taaatgcagc atcaagacta tttttagcat tgtttacttg tgtttgagaa accttagaat
     4501 cgtctagtac ttttttagca ttggataaag cagtctgaaa attattccaa gattcttctg
     4561 taaaatctcc tttagcagtt gattttactc ggttgtataa agcttgtaat tcagctttat
     4621 tcacagtagg aactggtttt ttctttaatc ctttgtaagc agaatctaat ttatttttgg
     4681 cactatccac ttgcttttga gtcgcttttg agtcatttaa cactttctta gcattgttta
     4741 aagcggtttg gaatgcgttc caggtattgt cggtatagtc accttttttc gttcccttaa
     4801 cgctcgtata aagagcttct agagccgatt tattcacttt gggtttagag gtgtcagcgc
     4861 cataccaacc gataccttcg tatttccagc catagtcttt ggttaaccat tgtacttctt
     4921 tccaacttgc agtgtagtga tgtgagccag attttacacc aggaaggaaa aggcggtaga
     4981 tcggtttatt atttttttcg gcagaacgcc aggcaggccc ttcataagtc cagccgtaat
     5041 ttttagtcaa ccatttcact tcatcccaac tagctgtgta atggtgatca cgaagacctt
     5101 tattgtacaa acgatagaca agatggccac cgctagtcgg tgcttcccag gcggggcctt
     5161 cataatttcc ccattttatc tttactagat ggtcacgttc ttttttgcta gaggtgtaaa
     5221 aatgttcatt attatttgga ttgtaaaggc gatacatgac gttagttgaa gcagcgtcaa
     5281 cagatccagc aagactaagt gaggcagcaa atgttccaat agctaaaaca gtttttggat
     5341 taaataattt cattttttca actcctaaat ttaattaaaa atgtttacat gattattata
     5401 tatcggctaa ccgatatagt caataaaaaa taagaaaagc cttatataat tttggctttt
     5461 cttattttat ttgttagttg aagtattaat atcaacgatt tcttcagcca atttatttaa
     5521 tttcatagca tttatgaatc tcatgttgtc tatactagag cgtttgttgg caaggtcact
     5581 tactgtagtt aagggtacgc cagattctat ggatatagaa cgaactgatt taccactatc
     5641 aaacagcaac cattcgattt tctttttatc agcgtacatt tcaatgctcc ttcttgtttt
     5701 ttctttatta tagctctttt ttgtaatgaa tcacaatatc aagagaaaaa ttttctatag
     5761 aaaggtggat ttttatgagt attcgttctc cgtgtatcca aatgaggttt gttgctactt
     5821 tcgttatttg atatactaaa aataaacaac gaaagaagga ataaaatgaa gaaaaagcaa
     5881 ctatggtttg tctctatgtt tgcaatattt tttattattt taagtggatg ttcaagtaat
     5941 aagaaatcgg aagctaaaga agataatagt ttattgcaac caggaactgt ttggaaagaa
     6001 gaagtaggtg gtcttattca taatttaaaa attatagatg atactacctg ggaatattct
     6061 gaaagtgtat ggcatccaga tcctgtacaa ataacagtta agaggcaaaa agactatgaa
     6121 ggtttagaaa gatacaaaat agtagattct gcaggcatta gggaatttat aaacaaacct
     6181 gattcattat ttattgttgt tccctatgag aaggatggcg taaaaaaaat aatttttctt
     6241 ggatctagca aggatgagaa gcgaacgaaa gagaaattaa ttcatgatgg atcagagtta
     6301 aataaataca aattacaaaa gactagtgag tgattgctag tctttttatt tatcctgaag
     6361 gaggaatcac agtggcaaaa gttaaaacac cattcgaaaa agccgcgtct aaaatcttag
     6421 aacgccgtgg tgtttctgaa atacagtgga aaaaagaagt tcttgaaaaa gcacaatttg
     6481 acctattacg aggaaaagat aaggacttag aagaatatat ctatcaacgt gaacgtgaaa
     6541 aacttgtatt cgatgaaatt attaaacatt aaaaggagcg aataatctta tgtttgaatc
     6601 aattacaatg aattttttaa ctcaaccgaa agtagtagct gcagttgata tttttagagc
     6661 attacaaact gccgcaacta gtttgaaaaa taatctgatg atttttgcgg ctgtagctgc
     6721 cgttgcctcc attgctcttg gaggtatttt cttcttcttt gggcgaagag gagctgaaaa
     6781 cgggaagaca cacgtaggtt atgtattgtt gggggtagga gttattttag ctgccgcttc
     6841 tattgtagtt tcaatgttct caatgtttgg acaaacccca caaggactat aaaaaaaaca
     6901 agtaggagga gtaaaaagta cgtggctact ttttactcct ttttttatga aaggagaata
     6961 ttcatgtttt ttacgatttc aggaaatgca aaattggata aagagttaat aagaaaagca
     7021 acgaagaaag tgggaaagcg tacttccttt ttaccgaaaa caattaaata ttctgtcatt
     7081 gaaacggaag aacagctaca tgtgttagga caagtgttag aacaagagaa tcaatcttct
     7141 gttaaattga ctctcacgtt acatgataaa gaaggaaaaa tagaaaaggc tggcacagaa
     7201 tacgtttttg gcccactgga ttatcaaatt tcagaaggtt tttatcctac attgatagat
     7261 ttaatccgcc ttgatgtgtt taaagatacg gatgaaacta tgagtgtgga agagtcttat
     7321 cgaaacgaaa aactgctaca agttttagaa gaagagttag agagcgacca aagaaaagtg
     7381 aatcaagtga gtgaagtcga agtaaatgaa gaagtaccat gggaacgacc aatctttcca
     7441 aagaaggagt caaaagaaaa agagacagtc tatgttgacg aacaagctga tattccgact
     7501 cagaaatcga tatttgagga agagaacgat gtgatgggtc aattggaggt agatcccttt
     7561 gaagaaacaa tggaggatga agagacggac gaagtagaag ttgatccagt tgaagctaat
     7621 gaagagtcaa catggactga aggggatttt caggaatata ttgaaccact tatcagtgaa
     7681 gaagtagaaa aaaacgaaac agagatacaa aaggcaactg tatttgatta tattttggat
     7741 caatatccgc cagagcatga atggatgaaa gaaaaaaaca aattattttt aaatgagctt
     7801 tattcgaaat atcaattacc ccaaacctat caagcatttt tagagagtaa agaagaatta
     7861 atctcacagg gtaaaggcac acttttagaa cagttagagt ctgtccaatc aacaaattgg
     7921 catgagcttg cacaagaaga tttattagcc acctttgaag aacgtcaaca agaaacagat
     7981 caagaaatta atgagtatgc gacggaacag tcgtctaatt gggagaaagt aaaagaggaa
     8041 ctaaataaag aagaacagcg agtgattgaa gaagaagtac aaaaagtcaa agagcggtac
     8101 gaaaagaaac gcgacaatgc ttattccttg tgtgtagaaa agatcaacaa ttttactaag
     8161 acgaatcaag aagcgttatc gaaagaaaaa gaaaacttgt taaaagaacg tattaaagaa
     8221 cgaaaagaag agttctaccg cggtttacgt acagataagc taaagattag caaagaatta
     8281 aatgaacaac tgaataagct atatcaaaca acgactgaag ttcttatgga aaaacacgaa
     8341 cagatccagc aagaattaca agtacaaatt ccaaaatgga aaaaagagta tgaaattcaa
     8401 caacaagaat tgaaagaaca agaagaaaaa caacggcaaa aagaaaaaga gcgtgaacaa
     8461 ttagaaataa gaaaacggga acaggatctt gcggaacaaa aaatggcgat agaaaaacaa
     8521 cggttagagc aagaaaagga aaaagaagca gctgctcgta aggaacgtca agaacaaatg
     8581 gatacattgc gtcaagcaca gttgaattat ctagcacaac ctcaaccagt agctgctgtt
     8641 caaacgccag tgaacaacca agcaaattta actccttcac cccagcgttc ctttaacgga
     8701 tggatggtgg gatgtattgc aagtttaagc ttgcttctag gtggaggaac agtatttgct
     8761 tttaatcata tcaatcaatc ttcacaggct gattctgtag ccacaacggc gtcaatcaag
     8821 gaagaagccc aggaacaagc aagaaaagag tatgaacaac aattagaaga attattgaat
     8881 cgtaccaatc agtcaacaac agagtcaagc atggaaaata gcaaagagag tgatcctagt
     8941 aaggctaaag aaggatcaag cgattcaact gtacactcaa acacttccga aagttcagaa
     9001 tcaaatgagg tgaaataatg cgtattagag aaacaacgag acgaaaagac aaacgattca
     9061 atttagaaaa gaataggtat ctttctcatt tctatcgcta tactccttca ggagaagagc
     9121 taccagaaaa acatgacaaa cgtatttctc aaaagatttt ttatagtggc ttagcttatc
     9181 ttcctttaaa ctatgttgtt acaagaatac cagaagcatt tcatcgattt gagattattc
     9241 caggaagttt acaaggagtg aatatttttc agttacaaga aatgccgcag ttattccatt
     9301 ttgattcaac tggtttttgg aatcctttga ccccaacaac aagtttaggc acacgtttag
     9361 ttgtttctac gatttgtgtt gttccagctg tacttatggg attgaaaatg gacatgtggt
     9421 ttcgtccgct agcagatgga caaaaagggg ataatcgatt aatgacctta aaagaaatcc
     9481 aacagattta tccgcaaata cctgaaacaa aaactacttt tccaggttat ggaggtattc
     9541 caatcacaca ttacaaaaaa tattggtaca ttcaaacaga tactgttaat acgtgtattg
     9601 ttggtacctc tcgatcaggg aaaggtcaga ttgaagtgtt agccaccatt gacaatttat
     9661 ctcgtgcaga aaaacaaagt tcaatggtcg ttaatgatcc aaaaacagaa ttatatgttg
     9721 gtagtaaaga tgaactagaa gctagagggt atgatgtata tgcctttaat atcaccgatc
     9781 cactgcaatc aatgagtgat aatccattgg cactcatcgt gaaatactgg aaacgaggag
     9841 acatagatac tgcaacacag ttaaccaata ctttcacaaa tacgatttat tatgatgcca
     9901 acgcttcaga taacaaatac tttaacgaaa acgcgcagaa agctgtcaat gcgcttattt
     9961 ttgcgttgtt agaacaagct gatcaaacag gagattactc caaattgaca ccgaataatc
    10021 tcgttgagct tctttctgag attggcggtt ttaattatta tccagatcca gacaatgaat
    10081 tcaaacagtt gaatgcctta gatgaattta tgaatcagtt gccacctgga aacattgcaa
    10141 aaaaacaata tggggcaacg aaaattggat ctgataaagc gaagggaaat attttatcaa
    10201 cggcaatcac gggattgaat ccatttactc taacgaaaat tgcaaagatg acttctcaat
    10261 caacgattga ttataaatcg gttgggtttc caaaatactt agatttgaaa ttacatgaaa
    10321 gtcttttaaa tcaacgagtc gtagtagaat tttatcataa gggaaagaaa tttcatgaag
    10381 aacgtgtcaa agtaggctat aaaggattta gtgaaatcaa ttttgattgt catttagttg
    10441 caggtgatca agtttgttta aaatacgatc atcaaggaaa agaatatgtg gcttggtatg
    10501 aattttctca aagagtattg aaagacgaag agggtaatat cgtttatcgg aaaaaacgag
    10561 gagaaaccca tttgccagaa ttagaaaaag aagccgttct aacattagat gaggaaaaaa
    10621 gtaacttatt agtagagtct attcggatgc actataccga taaaccaatg gctattttta
    10681 tgcttacacc tgattatgat ccatctaacc atatcattgt tagtattttt cttagtcagt
    10741 tatataaaga attatctacg caatgtgtta agactaaagg agataaatgt caccgcagga
    10801 ttcattttat tttagatgaa tttgggaata tgccaccaat ggataatatg gaaggaatca
    10861 tgacggtcac agctggccga aacatgttgt gggatctgtt tattcaatct tatcagcaac
    10921 tttatgcgaa gtatggagaa caaaacgcag cgattatcaa agaaaattgt cagacacata
    10981 tttatattat gtcgacagat gataatacca tcgaagaatt ttctaaaaaa gtggggaata
    11041 agacggttga acaagagaac agtaccatca attcattagg tatgaatact catatcaatc
    11101 gcaatgttga ttcagatcgt atcttaactc ctgaaagaat ttctactttt ttagaaggag
    11161 aaacgattgt actcgctcct ttaaaacgac aagatttgaa aggtcataaa atacggccgt
    11221 atcctatttt taatcatggg gaaacgaatc ttccttatgc ttatcaatat attgaagatt
    11281 tcaatccatc gtgggatatc aatgatatag acatacattg tacacatgcg gacttggatc
    11341 ttaaggcttt acaaattgat tatgattatt tcctaccaga tgaatatgct agaaatctct
    11401 atcatggact gcatgatcaa ttagaagaaa aaaacgaaga aaatatgttt tataaaatcg
    11461 ttcaatcttt tgagaatgag acggctaaaa aattgttaat tcaattttac gaagaacaag
    11521 cagcagatcg acttcaagaa tatgttgaaa agttgatcaa aaataaagaa atttctgaaa
    11581 tgattgggaa acggttgtta ttggaatctg aaaaggtggt gaaagaatga tcattggagg
    11641 aattttttct ggcatagccg actggtggtc aggcttattc ggagaagggg atgcaaaccc
    11701 caaaaccgta acgaaagtct tacaagatta tgggaattat ctgcagtatc aagatgtatt
    11761 tagttggatt tggaacactt ttctgtgggg attgattaaa ggattatata atctcaatac
    11821 tttactagaa aaaaccattt atcaaagctt tgatttaaaa gacttattga acgccgctgg
    11881 ggtaaatgca ctatttcaaa gtttgattag taaggtattg gctctttttt gtgtcgtcac
    11941 attaatttat ttaggcttga aattttttat gagtaaacac ccaccaaaag tcaaaaatat
    12001 cttggttaat ttatttatgg ccatgatctt gatttttggt ggtagttctt taatcgatga
    12061 agggttaaat atcagtaaaa gcttttacgg agacgtcact gcagcaaata aagcagataa
    12121 aacgggatca cctgcttttc aaattattca aaataatgtg atcgatgtca gtacgttact
    12181 agcaactgac ccgaataaag tagaatccct accaacagat aaaagaaatg ctttaacagc
    12241 gaaaaatttt aaaacagcga atatcaatgg gattattact cctgaacaag ctaaaggtat
    12301 ggcaggtaat gataaaagtg ggttatcaga tatacaaaag gatcggttaa aggcattaca
    12361 ataccgattg gaactagatg ataatgggaa agaaattcct gttcaaatta gtgacgaagg
    12421 cttagcaaaa tatgtctata cttcagggta tcgtcgatac agtagtaaac caggtattat
    12481 tttagctggt gaatcttctt tggcagtagc ctatttattt atcctattta cgatcgttac
    12541 ttgcattatt gagctggtat ttaaaaaatt ctatttagtg attgccgcgt caacggactt
    12601 tgagacaggt caacggatga aaagtgcgat agaagatatt tctcaatcct tcttgttatt
    12661 ggcgtttacg attttagaat tacgaatcta taccttaatg ctttcaggta tcggtgattt
    12721 gcatgcggca ggaaagatca atggattctt gtatgtcgtt gctttgattg tactaaccat
    12781 tgcgctattc aaaggctctc aatcagttac gaaaattttc ggcgttgata caagcctgaa
    12841 aaacggcagt aattctcttc ttagcatgtt tgccttagga aatattgcga aaaatgtggg
    12901 tagcggagct aaaaaccttg caagtgctgg taagagtggt ttaagccatt taaatgatat
    12961 tcgtaaaaat ggatttggcc atcgaaaaga tgttacccct gataacgggg aagaacaatc
    13021 agacgataaa gctacaactg tacctaatag taagaaaaca gtttccgaaa tgttcgggaa
    13081 agcgaaaaat ggtgtttcta aagccgcaga aggtcttggt tacgtcaatg aacgtgggtt
    13141 aaaaggaaca gctgaagatc ttacagaaaa agccaaagac accttagaag ataaattaga
    13201 cggcacgaga gctaaagaat tggctgaagc tgtgaagaat ccatccgaag cactagaaaa
    13261 acaaaaagaa aaagcttcag aagtcaaaga aggactaaaa gatgcgtatg gtgaaggaca
    13321 agtcaatgca gcagtgaaag ctaatacaac aaccgaaaaa gaaaatcaag aaggtatagt
    13381 gattccttca gtcaaagatc accaagaagc gttgcactct aatgaaaatt cgattgaaga
    13441 gaaaaattcc atagaagaaa gaaattcaga agaaggaaaa aatggacagt taccaaaaac
    13501 tgatccagaa caatatcgct ataaacctaa cagtgacctt gaaaatgaga agcgaatttc
    13561 agatttatca aaagtaaatc aagaagacaa agcagaaaat gagggacagt taccaaaaac
    13621 tgatccagaa caatatcggt atagatctgt taactccgaa aatgaaaaac gcatttcaga
    13681 tttaccaaaa cgaaatcaag aagtacaaac ggatgaggat tccatcgttc tgccaaatga
    13741 taatggaatc gaagttggaa ctgaaaaatc aacgaaaatt ggtttaccta aggcacaaaa
    13801 tttaccaatg aaagatagtc gtttgaatca attcaaggat aatgatcaaa gttttgttca
    13861 tcaatcacct gcaggtactg ttcaaatgaa accaagtcgt ttgcctgaaa atggaaatct
    13921 gacaactgtt tcttacaatt tatctgaaag tggtgagaca gggaatgcgt ccgttcaaaa
    13981 tgttgtgaca agagaagctg caccaaaacc tattcaaaac gttcatacag gttacaagcc
    14041 agcaaatgtg gcatttactg caccaacaaa aataaatgtg acaagagaaa caaaaaacag
    14101 gaacaatgca ttgaaagaac aactattgag ggatatcaaa caggatatca aaccataaaa
    14161 ggcctacaag ttaggccttt tttctatgta tgaaaaggag aaatcattta tggataaata
    14221 tagtaacatg aaaaaccctc caagatttag tggagaatgg aagtttttcg cttttttctt
    14281 tttagatcta ttaacgcttg gtggctgctt tttttgggcg aaaatggtca ataatcttgt
    14341 ccacttacca ctcttttttg cactcttgaa ttatggctta tggattgcga tagcgttagt
    14401 gattgtttgg cgaccctata ataacccagg tcaacgacaa atttttgcga ttatcgagtc
    14461 tatttttttt gcggacaaga accattatca tgcgttagat cctaaccgaa attatcatgg
    14521 tacgtataag gaggatgaat aaatgcgagt caaaggacaa aattcagttg aaaggaaatc
    14581 cattttgcag tttggaaaac cagaagagcg ctctatcttg gacttatttc catataaatc
    14641 ctgtacagaa gagggagtct taattactag agaagatcat tttcaacgtt attatcgtgt
    14701 agtttctaca gacgtggaag gattaaatga acaagagaaa ctagaacgaa tgaatcagtt
    14761 gaccaccgtc atgcgtacgt atgtgccaac aattaagtta gttagtttga ccacagagac
    14821 ggatctaacg gaacaaatcg tgcaaaagcg gcaattatta gataaaaatc gtatggaaca
    14881 gtccttagga agaaatctac aacgattaaa aaaatatgaa aagaaattag tcgaagaaat
    14941 ccaggaattg aaacgagcag aacaagaacg tcctgactta agcttctttt tcgtcattga
    15001 aggaaagaca atcaaggatt tacaaaccag aaaccgtcag ttaatgcgat ctagtggcat
    15061 tttagggtta aaaccactct acaaaaaaga attgatcaag attctgtatc gaatgaacaa
    15121 tatgaatgat gagtaggaat ggagaagaga gaatgaaata tccgttgata tggttagaaa
    15181 aagatgttcc gaaggaagag accactagaa aatttcctca cactttaagc ctgggtaagt
    15241 tgggaaaagg gcaatctttt aaccaagtaa agaatcaaac aagaagaggt gactgtacat
    15301 gttagaagca attaaaacag ctttttcacc acaaaatgat caaaaagtac ttgatccatt
    15361 aaagcaacta ggaaatcggg aaatagaaat tgtaaaatcc aaagggtatg atttagattt
    15421 tttaaaacaa gtgatgccac aaggtgggtt aaattttgat gaagaatatg tgacgtatgg
    15481 taatggttat tcacgatgtg tgacagttta tcactacaca aaaaatccta cgcttttttg
    15541 gttggccaat ctcatgaaca atcctcatac agtggccaca ctcgatgtta gcacagatga
    15601 aaagcaaaaa gtgattgatc aaatcaatcg tgccttagat gaattaaatg atcgatcaga
    15661 taatgaacga aaagcgactg accgaaataa aagcgcggaa gaatacctgg aactagcgga
    15721 atatgctaga aaattgactt ctaatgggga aatttctaag caagtcaaag tgagagtaca
    15781 tgtttacgga aacacgattc aagaagtaga tgaacgagcg caggaactga tcgaaaatct
    15841 caaagggatg gattatcgtg cggtgactta tctgcataaa accaaacgag attggcaatc
    15901 gatgtttacc atgttcgatg aacaagctga atggttcgga aatatcaaag ggcaatcact
    15961 accatcgtta aacattggcg gaggtttccc gttccaccac caaacgctaa aagatccagg
    16021 agggacacaa tatggggaaa ccatgacagg cggcccattt gtctggaatg cgacgtataa
    16081 aaatagtgta cggttgtcta ctaatacctt gcttctagga atgatgggag ctggtaaatc
    16141 gaccgttctt aaaatgatcg cggaagccca tttagctgca ggagacttta tttggggctt
    16201 tgaaaaggga aaagacttta ttcctttctt aaaggaatat aacggtatta tggtgcgttt
    16261 agacggatct gacggaatga tcaatccttt agagatattt gccacacgga cgtatgatga
    16321 agcatccagt ttatatgacg atggatcagt caaagatttg aagatcaatg aagcagcgtc
    16381 ctatcaaaca cacttagaca aagtggtcta tcaggtacag ttagtgagtc ctcagctaaa
    16441 aggaacgatg aaagcagaat ttaagaccta cttaaatcgt ttttatgaag agtatggaac
    16501 agtaccacgc ggctttactt cctcaaattc gcgttcaaat actgatacgc aagtcacagg
    16561 gaaagatcca gaagcttatc cgacgttcaa agaattttta gactttttag gacaactgga
    16621 attaccaggt gcttcccaag agaaaaagaa tcgaaaagaa gaaatggaat ccatcgtaga
    16681 atccttatgt gagacgtatg gcatgatttt tgatggtcat agtacgattc gacaccttga
    16741 ccaacaacag ttggtctttt ttgacattga ctctattaca ggcttagatc gaggggtaca
    16801 acaatgtcaa atgtacatgg ctatgacgtt gatttggaat caggcattga ttcaaggaag
    16861 aagacaacga cgtttaatta aagaaaaaca aatggaagaa caagacttca aacgcttctt
    16921 ggcgatgatt gatgagtgcc ataatggtgc gacctgttct gcagtgaaaa gctgtgggtt
    16981 gcaagtagtc taaaaactat tgtaataact cttataacga gaagtcaaat gagtgggtaa
    17041 cgccacaaag ggcttccact gatactccga catgcaacaa gtagtttatg cagaccaacg
    17101 ttgtgtgaag ctcggtgaag tcggctgaag gataccgtaa ggtaaaagaa atagtgaata
    17161 ccacgaaagt tctctagagg taaagggtgt atccgataag tcgggtgtcc aaaaaaactt
    17221 aatatggtga gtatgtacaa acgtactgac gaactagcga atgtactgtc cgaaaggcat
    17281 agctcctaga aatgggagag acagtgagca aaaaatcact cattgtgcta acttgtggtt
    17341 gagtaaaagt cgttgttatg aaagaccatg ctgcgttaca ggcgtaggag aagttagggg
    17401 gcaaaagcta gcatctaagt taaggcatgt ggatagatat aagggaacag gggaagccat
    17461 caacatggag agtgcatact cgatgaagtg gtagtaagaa atatgtgaat gacaaataac
    17521 ttactttagt gatggtgaaa gtagagtcac gactgatgaa ggttctgtaa tggaactgga
    17581 ggaacaggct cgattcgtct gtaaaagtca tgaaacccgt tcaaccatgg gattctcgta
    17641 tgactaaaaa ggtcacgagc caattggagc gtgataaacc atgacaaaag aaacaagtcc
    17701 aaaagatagg aagttgcggc atgcagaata ttatggcatg acggaaatct tcgataagat
    17761 atactccaaa agtttgaagg gagataattt caagaatcta atgcagatta tcatttctga
    17821 taacaacatt gtcctagcat atcgtaatat caagagaaat cacggtagtg tgacaagagg
    17881 agaagatgga gtaacgatta aagatattga aaatctcaac cgtaatgaat tcgtagaaat
    17941 tgttaggaag cgattcgcac attataaccc tcggaaagta aggcgtgtag aaattcctaa
    18001 atataatggt aaattaagac cgttgggtat tccatcaata tgggatagaa tcgcgcagca
    18061 atgcatctta caagtattag aaccaatatg tgaagcaaaa ttcaataatc acagctatgg
    18121 tttccgccca aatagatcag cagaacacgc aatagcagat tgtatggccc gaatgaatca
    18181 aagtcacatg gaatatgtgg tagatgtaga tatcaaaggt ttcttcgacg aagtcaatca
    18241 tactaaactc atgagacaaa tctggtctat gggtatacga gacaaacagc ttctcttaat
    18301 cattaggaaa atgttaagag cacccgtagt acttccaaat gggaagttga tatacccaac
    18361 aaaaggaaca ccacaaggcg gaatacttag cccattactt gcgaacatca atctcaacga
    18421 atttgactgg tggatagcaa atcagtggga ggaaaaagat gccaaagagc taaaacatca
    18481 ttttaggaaa aatggagctt gggataaagg taatatctat cacaagctca gaaagagtac
    18541 aactctaaaa gaaatatacc tagtgagata cgcagatgat tttaaaattt tctgtagaaa
    18601 ttataatgaa gcgaaaaaag ttttctatgc aactaaaatg tggttacagg agcgtctaaa
    18661 gttaccaata tctgaagaaa agtcaaaaat cacaaatctt tcaaaagaag aaagtgaatt
    18721 tttaggcttt acgatgaaat tagtgaccaa aggtaacaaa aaggttgtta atacgcatat
    18781 aggtacaaaa gcacaaagca tgattaaagt gaaactaaaa aaacaaatca aacgaattca
    18841 aaaaagtcct aatagcaaag atacctttat ctatattgga gtctataaca gtatggttat
    18901 tgggattcat aattacttca aaattgcgac tcattgtagt gtagcaatgc gaaaaattgc
    18961 aagagaactc agaccaagtt ttgaaatacg actacgagaa caaggaatct caaaagaagg
    19021 acaatacgga aagtacgaag gatacaagcc ttacctacgg tcaggacaaa tacgatatat
    19081 aagagggttt ccaatattac cagtagggta tattcaacac agacatccga tgtgtaaaaa
    19141 aagaaatatt aataaataca ccgaagaagg tagggaagcc attcataaga agcaacaaac
    19201 cgtagaagaa tggaagatat cgtggttgtg tagcaatcca gtgcttagta agagagcaac
    19261 gattgaattt gcggacaatc gaatttcaaa attcatcgct caaaagggaa gatgtgcagt
    19321 aacaggtgag gagcttatct tatctgaaat gcattgtcat catattatcc cttatcatga
    19381 aagcaaaagt gatagctatg aaaacctagt gattgttact gaggaggttc atcgggtaat
    19441 acacgctaca caaagtgaaa cgattgagga acttctaaaa tatctaaaat taaatccaaa
    19501 acaaaaagaa aaactcaatg agttgagatt aaaagttggt aacgaagaaa tatcttaata
    19561 aatatttgta ggacgggaaa acagaatgtc ttttatagac gagggaacgc cgtatgcggt
    19621 gaaagtcgca cgtacggtgt ggactggggg aaaagacgga gataatatca aactcttacc
    19681 tatcagtatc gattaatcca gattttattg aaacggtaaa ttatgtcaca aactttcaac
    19741 gagaaatgcg taaagtagaa gcaatgactg ttttagctac acaatcccca caagaaatgg
    19801 tgccagaaaa tatgtcttct gataacttct cgaaactgaa gaaagtcttt gaattttctt
    19861 ctacgaagat ctttatgcgt atggatgaat ctattttacg tcatatagaa ggattagtgg
    19921 gtaaatcaat gacgaactca gaattagaat ccattcctca acaaaatcaa ggagacgctg
    19981 tgatcaattt tggctcaaaa gaaaccattc gtgttcattt tagtccgaac aaacgtcaat
    20041 taaaattgtt tgatggagga aaataatgga aaaccaaaat gaaagtttaa tcaagcagta
    20101 tgttaagcgt cgtgcaaagc ggcgcttatt tttgtggttg tttggtactt ctgcaggttt
    20161 gattactatt ttgatcactg tgttcgtgac cctatttcta atacttgcgg cgggctcgat
    20221 tgataattcg gattcagatt caagcagtgg tggcgaagct tttacaggag aatattcgga
    20281 aggcttaccg atttataaag aaatcaaagg aagaggccct ttttctgatg agattgcgca
    20341 atatgccgta ggtgcagcag tgaaatataa attattacca agtgtgatcc tttcgcaata
    20401 cggttacgaa tcagcgtttg gtacatcggc gtccgcaaga aatgatctca actattttgg
    20461 gattacttgg tttgatggtt gtctgtttcc taaaggaact gcacgaggaa ttggtggcat
    20521 tgaaggtggc tggtacatga agtttcccaa ctcaaaagct gcattttcct attacggctt
    20581 tatggtagcc acacaatcca attttaatgc ttgtgtagga aataaaagcc ctggcgcaag
    20641 cttattgatc ttaggacgag gcggttatgc cgctgcagga attacggaag atagtccgta
    20701 ttatacaggt tgtatgtcaa ttatcacttc taataaatta accgaatatg atgaattcgc
    20761 catcaaacat tggggtgaag gtggcaataa caacggcacg attactggtg aatggacgaa
    20821 tccattccct ggcagcagtt tagataaaag ttccttcagc ggtggtcaat tatttggaac
    20881 gaatccaggc ggtgaattcc gtccgaacgg cttccatgat ggattagatt ttggttcagt
    20941 tgatcatcca ggaagtgaga ttcatgcggt tcatggcgga aaagtcgtgt acgttggcaa
    21001 tcctggtatt tcaggtttgg gtgcgtgtgt gatcgtgatt aattacgatg gcttgaatat
    21061 ggtgtatcaa gaatttgcaa atagtacagg aaattctcga gtgaaagtag gcgatcaagt
    21121 gaaagttggt caagtaattg ggatacgaga tacagcacac ttgcatttag gatttactcg
    21181 aatggattgg cgtcaagcac aaggacatgc attcattgat gatggtacat ggatcgatcc
    21241 gttaccattt ttgaatagca gtaaaaaata gatcaggaga aaaaatatgg aaaataaaac
    21301 gttaaaaatc gtcttaacag ctgcaattag cgttagttta atcggcaata ttgcgttgtt
    21361 ttggcagctg aaagaggaac aagcagcaaa tacctataaa attgaagaag tggctaaaaa
    21421 cgcgtctgag aagctcaaaa aaaattcctc tgaggcacag gcgaagatcg acaagttaaa
    21481 tcaaaccatc aatgagaaaa accaagaaat cgatcagctg aagcaacaag atggcacaaa
    21541 agcaaaagag gaagtaactg aagcacaaag aaagacggca gaagagttcg gtcaattggc
    21601 cattgataaa tcattaagca gaaatgaatt aacggaaaaa ctaaaagatg tcgcaaccca
    21661 agaagtgatc gacaaattaa gtcctacaga cgatgatcac caacatgatg attacggaag
    21721 ttactccttt acattaggtg acgtgcaaac ttacgctcaa acaagcagtg taaaagagga
    21781 acaaaatttt gtcgtatttg ttgattatac aatcggcaat ccgcaattca aagatgtcaa
    21841 accacaaaaa ataaaaggtg gattaactgt cactgaaaaa caagtagatg gtcaatggaa
    21901 agtcatagac tttagttatt tcactcgtta ggaggaagaa aatgagtaag aaaaaaggag
    21961 aaagacaacg tttcaaaaaa cgaaaaagtt atcttagatg gctgggaatc tttatgattc
    22021 tcggcttttt gattcttgct agtccgattt atacgaatca gaaggtagat ataccagatg
    22081 aggtaagcac gaacgccttt ttaaacacga aagagctgac aatggtttca aaagaaaaag
    22141 gagcgaacca gcaattagtt tttgaatttg cgatcgatga tgaagatcaa agcttgttaa
    22201 aagaactggc taatcttaat tatcgcgtag aagtaaagac agctaaaggg gattatcaag
    22261 caatacgagc caaagtgatt aaggtgtctg atgattattt tgcagtgaag ctaacgcatg
    22321 taccagaaga atatatcgcc atgcgtttaa ccattattcc tgaaaaaatt gatccaaaag
    22381 tggatatgca agaaccacaa gatctgattt tttatattca cgaagataag gtcaaagatc
    22441 acgtgaaaga cgaagattat gaacaacatg cgatcaatta caaagtgaaa ggatacaaga
    22501 aagaacaaaa agcagctcaa aaacaaatgg aatctttcca agcaaccatt gatttgaatg
    22561 aagaattagt gaagaagcta aaagatcaat tgccttatca agtcgcagat gatcaggaaa
    22621 atactgaaaa caagattaat agctaccagc aagaaattga aacatcgaaa acgcaactga
    22681 aagatcaaga agagatcatt caaaaacttc aagaaaaaat tgatcgtatg acaaaagaaa
    22741 agatttagaa ggacaggtgg agtgaaaaaa tgaagcgaac aaacttggct gacaaagata
    22801 ctttcgctga gcagaaagta ttgattaaag cctatctcaa agttgtaaaa gctaaagaaa
    22861 tatgtcgaga agtgaattta tctaacacaa caatatctga gttgaagaat ggtcgaaaga
    22921 atttagatac cttaaaattc agatcatttg aagctctata tgtagctgct aaaaaatatt
    22981 ttacagctga taacaaataa aaaacaggag tattttatgt gaaaaaagta tataaaaaat
    23041 gcttatgaat cagctatatc accttaacca tttgttaatg agatgggaga aaacattaac
    23101 cacgattcta acgcaaacat caagcacata aaacatttaa cactcctcct ttctggctga
    23161 agatttcaac caaacaggaa gacagcgtat caattatagc atacactttt tcttaaattc
    23221 atttttcata tgacaaaata tttccttcta atcgacaaag ttagaaggat tttttattgt
    23281 ctaaatacac aaataacctt aatttttaaa gagcataagg aaacgtgata tcactttctc
    23341 acgaaaggtg ttaggtaggc tgtgcctacc acgtttctaa gaaagataaa tgatatcaaa
    23401 attttggatt tgtgtgtaga tttgtgggta ggatgaaagc gaatgtgggc agtaaaataa
    23461 gcgtttgtgt gtagcaaaat caaaaaatgt gtagggatat gtgtagctac acatatccct
    23521 acacataata aatttggagg aaaatatgga cttattaatt agaaatattg aagaaaagta
    23581 tattaataaa attgacaaac gttgtgaaga acttacggtc aagactggca aaaaatggag
    23641 tcgaaatcaa tacctaaaag ttttgatcga aaatgatttt gatcacgctc ttctgaatta
    23701 taaaaaagac caatttgatc ggttgttaga aaaatttgtg gatattcaaa cttataatac
    23761 gaaaacttta gaagaatata ttgcaacaaa taatcaatta attggtttgt taattgaata
    23821 ataaaaatag tattgtgaat ttataaaagg aggaaatcaa ttttggcaaa agcaacaaga
    23881 aaacatgtat atctttacga tatgcatatc gaagtacttg atgaaataat ttctactcat
    23941 gaagatatat ctaattatag tgaagcagtc agacatttgt gtatgatgta tgatccaaaa
    24001 atcgaagaaa aaataaatcc aattcaaatt aaattgaatg caatgggaaa agaaatttct
    24061 attttaaccg aaatggtttc agagtttgcg gatcttcatt tgaaagatac aggaatacta
    24121 attggtacaa attcaactgt ttatcaagaa gcagaagaac gtgtggaagg aaaaattaaa
    24181 tccaatcaaa ccaaaaaata ttcttctaag ttaaagaaac cagtgaatga atctgttcat
    24241 accagagtag atccatttga tccaacgaag attctaaaaa gataaaagta gaaggagaac
    24301 gtgatgaata aatcgcctgc agttattctt acttgtcaat ttaccttgcc caataaaaaa
    24361 tctttttcaa catacattga ttatatgaca agagaaaaag cactggaaga aatggaacga
    24421 cgtacgcccg aagaagaaca ggaactgcaa cgaatcaaga atgctttaga ggatttttat
    24481 attcctgaag gagaaacttt ttcggaaagt aagacggata aagaattcag tgaaacgtcg
    24541 aatgaagctc aatcgttgat ggaacatgga atcaattttg acaagttaga ggaacaagat
    24601 tttaccaaat atctttctta tatgacccgt cactatgcgt tagaacaaaa gaaagaactg
    24661 acgcaaaggg aacaaaaaga ataccaacgg ttgaaccaag caatgaaaaa atatcaaaca
    24721 gagccacata aaaaagcttc aggaaatctt cctggagttt tttctatggc ttcagacgaa
    24781 gtgaaaggaa gtgatctgaa ggagatcaaa caaatcttcc aaagaggaca acaaaaaggc
    24841 tcaatcattt atcaagatgt tatctctcac gacaattcgt atcttgaaaa aatgggcttg
    24901 tacgatccca aaacagatac cttagatgaa gaaggtttga aagcagctgg aagaaaaatg
    24961 atggagacat tattcgagaa agaacaattg aatgaaacag gttactggat ggcaacgatt
    25021 caccgtaaca ctaagcatat ccatattcat tttgcagttg ttgaaaaaga aaatacgcgt
    25081 aaaccgtata ctgagattga aaatggtgtg agttatatcg taccgaaggg gaagagaaga
    25141 caagcaacat tagacgcaat gaaaaccaat tatgtacatg agttagaacg tttctcttat
    25201 gaaaaggatc gaatgatcgg tatggagaaa cgaaatttat tgacacgaaa aagtgatttg
    25261 cgtaacacct taacaaaagt agtaaaagaa ccagcacgtt acgatcgaca cgccatgaac
    25321 ttattaaatg aagtatataa aaccctacct gaaaaacgtt cggaatggaa ttatggagat
    25381 gaaaatcgaa ccaagctttc accatttaca aggggaaaac ttgatgagtt aaccaaatat
    25441 gttttaacaa aagaacctgg atatcaagaa tatatacaga tttcaaaagc attgaaagaa
    25501 gaagccaaaa gcctttatgg ggaaagcaaa agagaaacaa agaatgcaga aaaaaacgcc
    25561 ctctttgatt taaaaaagcg cacaggaaat gcgattttaa cagaactgaa gagacaggat
    25621 agtcaaataa aaccattaat agaaagtgtc aatcagatgc aatctattta caatgaacga
    25681 tcctttcgaa cactcaaaca gttgccaaca aatgaataca agtatagtta tcaagaattc
    25741 gtaaagcgta ggaaacaatt ccaacaaaga gttgtcacgc agcgaagctt gcgtcaatta
    25801 aatcgaacaa ttaatgcaca tgaagaaaaa taccacgcaa gtaaagcata tgaagagcaa
    25861 cagcgaagaa tccaaatgga acaagaaaga caataggagg aagagaaatg aaacaatatt
    25921 tagatcaatg gaaagtcata gaaggatcac taaaagaaga acgaatcgaa caacttcctg
    25981 attgtttaga aaaagagcat ttgtttcaga taagcgaaat gttgagaaac gaacagtttg
    26041 atccaaatca atttttagtt gttgagtacc cagctactgg tgtgtattgt tgtaatcatg
    26101 tgaagggtga aaaatatttt attattcaag aatacgaagg taagttagcg ccttattata
    26161 ctacttggga aataaatgaa gagggaatca ataatttccc atgtgaaagc attgaagagt
    26221 ctatttctct cacggaatgt taaataaata gagacgtaaa agagtcctga acgtgattgt
    26281 ccaggactct ttttcaatga attgttttcc gccaattcac tgagtggtgc gattcaattt
    26341 tattatatca aaagaaagtg agattccaaa gtgaagggag gaagaagaaa tgactcagac
    26401 aacgattggt gttgtggttg aaaaaagcag ggataatctc gtttttgtga cagaaattca
    26461 aacaggtaga gcttttgtag tcacagacaa agctgcaaaa gcgtatcaaa atggcaatat
    26521 tttaacgtta aatctgacta cgaagatgtt cgttgatgca gtagaaaatt atccatttgt
    26581 ttagacaaac gatattttac aaggaggaaa cggacatggc agaagaaaaa gaaccactag
    26641 ataatagtcg gttgggaaag tccaaaagaa aattagtacg gttgcaaaac gaattaaata
    26701 atcaatttca aaagctattt gatcaccaaa aattgacaaa tgggcaacca atgaatgaca
    26761 aacgaaatgg tcgttcatgg tttaaccagc aagaaaaaat agaaaataaa atacaaagtt
    26821 tgagagaaga gatcaagcaa caagaaaaac aggtagaaaa acttgaaaga caagaagagc
    26881 taaaggaaat gggctataac aagtacggcg gactggatat gacgatcgat aatattccaa
    26941 gaatcaaaga agaaatcgaa cgctttgaaa aaggtgaatc gactttttca gccgccacga
    27001 tccgaaaata tcaacggaag ttggaaacat tagaacagct gaaagaaaaa tcagaaaaag
    27061 gaaaagaaaa tatcctccct gaagtacaag cgatcattga cagcggacga gtgacgcaat
    27121 ggaaaaaaaa tccaaccatt tattttttga aaggctatcg aaaagttgct ttagaactga
    27181 atgaaaacgg gggatttgag gaatcaaaaa aataccaagc cacgtcggaa gaggaaaaag
    27241 cagctgttcg tgaattactt gaagaggctg caccaactga ttctctgaag gagaaagaaa
    27301 aagatcattt ctatcgagta gaattcaacg aaaatgatcc agaaaaagga ttgaaaagtt
    27361 atggtgggga gatcgtaaca ttagaattga tcgatcaact taaaacgttg gataaccaat
    27421 acgctgcaga agcaggttat tataaattct attttgaaga agtgaagaac ggagaagtag
    27481 tagatcagat ccgaatggat ctaggggatg gcgtcaacag taatcacccc atctatttag
    27541 agttagcaaa attgtgcgaa caagtacctg agaaggaaca aaaacaatcg gtcaaaaatg
    27601 aacctgtttc gaaagaacaa atgcagtcga aagaaacaaa aacaaaacaa aagggctatc
    27661 aaaaacgaac ggcagaagag atcaaacaag aagttaaatc cctttcgcaa caggcactgg
    27721 atgcggtgaa aaagcatacc cattcttctg aagacgtcaa agaacttttg aactttatgt
    27781 ctcgttttcc agaacgatcc tttagaaatc aattattgat tgaggaacaa tttccaggtg
    27841 caacggcttg tttaggtcgt gccgcgttga aaaaagaagg aatctatatc aaaaaagggg
    27901 aacaagggaa caagatcttt gtcagaaaaa cggtcaaagg attttatgag aaaggacgtg
    27961 gctttgttcg agaaacagaa gctacaccag aagataaaaa acgaatcgaa agtggtcaaa
    28021 tcgaagtgat caaaaaacct tactacacga ttgaaaaagt gtttgatatc acacaaacac
    28081 agttaaaacc tgaagactat ccgaaaatct ttccgaaccg tgtctttgat tttcaattgg
    28141 ataaaaaagg acagtcagaa cttcaagctg ggattcaagc ggtagccaac aatattggaa
    28201 tacaaattcg agatatgaca gaaagtgaag tctatcaaag agaattagga caagcacgtg
    28261 gggcgtatgt tcgtaacgaa tttactggcg aagaagaaat cgtcatgaat actcgaaata
    28321 ctcctacgca gcatttagct acaagtattc atgaattagc ccatgcaaga atgcacaaat
    28381 tttcagaatt agataccgca actaaagaat tgcaggcaga aatgacttct tatattgtgt
    28441 gcaaacattt tggaatggac acttcggaga aagctattcc ttatattgca gcatggacga
    28501 agaatggtca gacgttggat gataaggaag cggcggaacg tgggaaaatc atgaatgatg
    28561 tcagccgtgt cgcaaatgaa ttcattcaaa ccatttctaa tgaaatcaat caatacagag
    28621 aaagagaacc agagatccaa ccagcagaag tgaagcagga gcataaaaat actgtagaaa
    28681 cggcagagaa taagaatatt gtgcccgttg gttcactttg gatcgacaag aaagacggga
    28741 aagtaatgga agaagatacg ggtcgaacgt tacatttgaa ggatgcggcg tttattgaag
    28801 atccgcagca taatcaagtg attttaaaag gaaaaagtta tggaaaagag attcaaacgg
    28861 cgttaccttc agaagaattt gcgctaggaa atccattccg attttcactt gaaacagtgt
    28921 cagaagtggc caaacgaaaa aatgaaatct attatgaaca acaattgtca caaaaagaac
    28981 gcgtataaaa caagtaaaaa agactatctg aaaaacggat agtctttttt cttttgggag
    29041 gtttgaaaat gaccgaaaaa aaaatgagtc ttattgatcg atgtaaacag atcgatatag
    29101 tggattttgc tcgaaacaat ggaatggctg tagtcaataa aggacgagac tatcgcttag
    29161 aagaccatga ttcatttgtt tttgatcgaa gaaaacaacg tttttattgg aacagtcaaa
    29221 atattagtgg cgacattatt gaattagcta aacttttttt catagacaaa gagatccaag
    29281 actctaagca acaatttaaa gcagccactg attttattct aaagaatgaa gacaaaacag
    29341 aacgagtgga aaatcttcat tttgaaacag aaaagtacaa agatcacccc gttgattatc
    29401 aaccactcac agaaaaagga aggaattatc tgaaggaaga acgaaaacta ccagagtggc
    29461 tgattgatta tgcagaaaaa gaagggttga ttgccgaact aaagccaaaa tatgaacggc
    29521 aaaatttctt agttcgagac gatcgtttag atcatgcggt cgccttttta tggaaagatc
    29581 cacagacaaa agagacagta ggggcgagtt atcaaggaac gttcattgat tatgaacgtt
    29641 ttggtgagag aggcacatat aagcatatag ataaaaattc gacagcgaat catggcttta
    29701 acctaaagat cggtgaccct aagcagttaa aattttttga gagtagtatt gatttactta
    29761 gctatgctgc actgaatcgt gatcagttaa acgatacgtg gttagtctct atggaagggt
    29821 taaaacacca tgtgatcagt cattattttg gtgaggcagt atctgaattg agaaagaaac
    29881 aagcctttcc tcaatccatc gagatttgtg tggataatga ccgtgcagga catattttct
    29941 acgaaaaaga acaattaatg ggcgcagtcg atccatttac gaatcagaaa gtacgttgtg
    30001 aacgaggaat cgctaatgat tggcaagtgc caaaagaata caaaatcatt tatgaagaag
    30061 tcgcaaaaga agaaaaggtc acaccagaag ctattatggc catacacaag acagaaaaca
    30121 atctgcaatt aacgaaccaa ctggtttccg ctcacaaagt aaatgcgttt tttggccagc
    30181 agctttctgt taacgattca atagaagcca ttaatttgaa agatatttgt agagaagtcg
    30241 caaaagaatt aaaagtttgt gaacgcgtgg atggtacgta cgattttgat cgtttttatc
    30301 aagaaaaagg ggatattaat gcgcagattc ttttctctta taaagcagaa caatactaca
    30361 aaggctataa aaatcatgaa catgaattta tacctgaagt aaaaaaggac tggaacgatc
    30421 aattgaagca tgagatccag caacaagaaa tcagaaagca aaaacgtgcc atgttgtttc
    30481 aacaaggcag acaacaagaa agggagtgaa atcgtcatga tgaaaaccgt tattttagca
    30541 gaaaagccgt cacaagcgaa agcttatgcc gatagctttt ctaaagctac ccgaaaagat
    30601 gggtattttg agatccaaga tcgattgttt acaggagaaa ctgtgatcac gtatggtttt
    30661 ggtcatttag tcgagttaga ttcgcccgat atgtacgatg aaaaatggaa acaatgggca
    30721 ttagaacatt tgcctatttt ccccaatcaa taccactatc atgttcctaa agataagaaa
    30781 aagcagttta gtgtcgtcaa acgacagctg cagtcagctg atacgattgt gatcgcgact
    30841 gatagcgatc gtgaagggga gctgattgcc tggtcaatca ttcaacaagc aggcgccaat
    30901 caaggaaaaa cctttaaaag actttggatc aactccttag aaaaagaagc catttatcag
    30961 ggattccaac agttacgaga cgcagaagag acctatccaa aatttgaaga agtacaagca
    31021 cgtcagatcg ccgattggtt aattgggatg aacggtagcc cactgtatag tttactcttg
    31081 cagcaaaaag gcataccagg aagtttttct ttaggtagag tccaaacccc aacactctat
    31141 atgatttatc aattgcagga aaagattaga aacttccaaa aagaaccata ttttgaagga
    31201 aaagcccaag taatcgctca aaatggcgca tttgatgcga agcttgatcc aaatgaaacc
    31261 caagcgacac aagaagcatt tgaagcctat ttaaaagaaa aaggtgttca attaggaaaa
    31321 cagccaggta cgatccttca agtcgaaaca gagaaaaaaa gtgcggctag cccacgtctt
    31381 ttttctttat cgagccttca gtctaaaatg aatcaactca tgaaagctag tgccaaagat
    31441 actttggaag ccatgcaagg gttgtatgaa ggaaaatatc taagctatcc gcgaacggat
    31501 accccttata ttacggaagg ggaatatgcg tatctgttgg atcacttaga cgaatacaag
    31561 cattttttaa aagccgaagc gattccgact cctattcata caccgaatag tcggtatgtg
    31621 aataataaga aagtccagga acattacgcg atcattccta caaaaacggt tatgacagca
    31681 gcggcttttg aacaactaag tccactgcag caggctattt atgaacaagt gttaaaaaca
    31741 acggtggcca tgtttgcgga aaagtatacc tatgaagaaa caacgatctt gacgcaagtt
    31801 cagcagcttc agttaaaggc aatcgaaaag gtgccgctag atctaggttg gaagaagtta
    31861 tttggaaaag agagcgacgg aaaagagaag gaagaagagc cgctacttcc taaagtgaca
    31921 aaaggagaaa tggtgacggt ggatctacaa gttcttgaaa aagaaaccaa gccaccacag
    31981 ccttataccg aaggtacgtt gatcacggcg atgaaaacag ctgggaaaac cgttgatagt
    32041 gaagaagcgc aatccatatt gaaagaagtg gaaggaatcg gtacagaagc gacacgggcg
    32101 aatattattg aaaccttgaa acaaaaggaa tatatcaagg tagagaaaaa taagctcgtg
    32161 gtgacgaata aagggatttt gctttgccaa gccgtggaaa aagagccgct gcttacgagt
    32221 gccgaaatga ccgccaaatg ggaaagctac ttgttaaaga tcggtgaacg aaaaggaaca
    32281 caaaccactt ttctagccaa tatccaaaaa tttgtctctc acttattgga agtcgtacca
    32341 gggcaaatcc aatccactga ttttggatcg acgttacaag aagtgaaagc agccagtgaa
    32401 aagcaagaag ccgcgcgtca tttaggaatt tgtccaaaat gccaggaaca agaagtctta
    32461 ctctatcaaa aggtggcagc atgtacgtct gaagcctgtg atttcaaact atggacaacg
    32521 attgccaaaa agaaacttac agcgacacag ctgaaagaga tcattcaaaa tagccgaaca
    32581 agccaaccag tgaaagggtt aaaaggtcag aagggttcgt ttgaagcaac gattgtttta
    32641 aaagaagact tcactacagg ctttgaattt tctgaaaaga aaaaaacgaa cttcaagaaa
    32701 agaacacgac gcactacaaa ataatggagg gttaatctta tgcgtaatac aacacacaaa
    32761 caacaagaaa tcgaaaatca aaaagccaaa attcgttcag aacaggaaaa aatgcgtgcc
    32821 agcaaacgaa gactgcagca attgaagaac cagcagcgga ttcaaaaaga aatcgaatta
    32881 gatcgataaa ttcctttctt gttaatataa aactaacatg ttatagttgt gttagtttta
    32941 tattaacttt aggggaagag taatgaaaga caaaatccaa tgtatttttg ataaacataa
    33001 tggcaaccta tccttgaagg atgctagaag tgaaggatta tcgcctatga cgttagatcg
    33061 attagccgaa aaggataaaa tagaaaaggt agcaccaggt ttctatacct taaaggatgc
    33121 gttcgtggat gaattgtatc tcgctcagag tatctattcc agagggattt tttctcatga
    33181 gactgcgctc gatctttttc aagtaggaac atatatccct aaaaagatca atatgatgtt
    33241 tcctaaagga tacaatgtat caaaggagaa attagatcag tatgccattc aaccgcatta
    33301 cactaataaa gaaaattttg acctgggagt cacagaaaca gaaagctttt acggcaataa
    33361 aattttagtc tatgatttag agcgaacgtt atgtgacatg tggaatccaa gatacaaagc
    33421 aagcgtagag gtaaaacaag aagcattaaa agaatatatg acaagtacca gtagaaatac
    33481 aaataagcta agaagataca tggatatttt aaaatcacca aaagaaatga ctttgtatat
    33541 gttgccactt tattgagagg agtaaagatg aatgacaccg acacaattta aagagaaaac
    33601 aagaaatttc gcagcagaga aacatttaaa cccacaaatt gtccaaagat attatatgat
    33661 ggaaaaatta ttagaatgta ttgttgaatc gccttatcat gatgattttg tattaaaagg
    33721 gggatttctg attggatcaa aatacgggtt agaaaaccgt tcaaccgttg atattgatac
    33781 gacttatcga aattcgaaac taacaaaaga aaagttagaa actgtttttt ctgaattaac
    33841 agctaatcct acaaaagatg gaattcgttt tgaattaaag ggattaacag agacgaggga
    33901 agccgattat tatccaggtt ttcaagcaaa gtttattgct tatcttgaaa atgcacgtgt
    33961 accttttaaa ttagatatta cttcaggtga ttctattatt ccagcagcat tgatgtatga
    34021 acacaaatta atgtttgaag ataaaacgat tcatattcca gcttatccta cggaacaaat
    34081 tctagctgaa aaattacatg ccacgtttag ttttggaaaa gataattcgc gcatgaaaga
    34141 tttttatgac ctgtatatga ttccgaaatt agaacgtatc gatgagaaaa atttgtataa
    34201 gagtgttcaa ggaacattca aggaaagaaa taatcaatcg gtgtttaagc atttgtatga
    34261 tcaggaaatg cctcaattgg tgaaagatga gggcttgaga gaaaagtgga aaagatacca
    34321 acaagaaaac tattttgcca aagacatttc tttcgaatct acactaactt ccgttgattc
    34381 cctgatgcaa tcaattgtag agcaagagaa ggcagaacga aaacaaaatt ttaagagatc
    34441 acaacaaatg aatcaagaaa tggagagata aagatgatca ataatgtaac tttagtaggg
    34501 cgattaacta aagatccaga tttacgttat accgcaagtg gcacagcagt ggcgactttt
    34561 tccctagccg ttaatcgtaa tttcacgaat caaaacggca atcgagaagc tgattttatc
    34621 aattgcgtga tttggcgcaa accagcagaa acgatggcga cattagctaa aaaaggcagc
    34681 ttgatcggcg ttgtcggacg aattcaaaca cggacttatg acaaccagca aggacaacgt
    34741 gtgtatgtga cagaagttgt ggccgataac ttccaactat tagaaagtaa agcagcgaca
    34801 gaaagccgta tgcacgctga tcaatcgtcc agcagtccaa gccctaccac ctttgaacaa
    34861 cgggatacag cgacgcctaa caacaatgga ttgaacgctt cacagaatcc atttggtgga
    34921 caatcaatcg atattagcga tgatgatcta ccgttctaag tagcattttc tattggttgc
    34981 atttgctacg gtttatgtta taatttagac aacaaaaaga attttcaatc tataaccaaa
    35041 aaccactagc tggcgggcta gtggctttct tttacgtttc tattataaga attaacgggt
    35101 aggcttactg tctttcttgg caaatttaca atcaagccat ttgcagatat agtgcgcggc
    35161 tacttctgcc atgattgcaa aaacaaaatc aaaaagaatt ttcaaaccta taacctcctc
    35221 cccttgccag attaggccgg ggtagcagac agcaagacta ttatagcata attgaaatat
    35281 ccaaatgatt ggatttttgc tataatttta gcgaggtgat tgtgatgaac aaatatgttg
    35341 gtttgcttga taaaatccga gtaataaaaa cgcaaccgtt attggttcgc tttactttac
    35401 aaacgattca tgaatcgatt aattgtgtag ttgcagatat tgaaattatc gataaattgc
    35461 taattatgga tgatggaaaa tacaatattg ccgtcacagg tcacttcaat aaacgaaatc
    35521 agctagttat tgctagcatg tatgttagaa atcccgatca ttttacgcga tcgatgggca
    35581 tataaataaa agaagagttg tagtcctaaa tgaggattgc agctcttttt gttttacata
    35641 gaaacaggaa aagacctttt taggaagaca caagtgcctt ctcaattgaa aaacctttct
    35701 ctcactccaa ccgccaccgc cgttcgagaa gaaaggaagt gaacgctgca cgttcactaa
    35761 gatatggtgt cttgcaaagc aagacaaaat agacaaaact tcctctcacc aaaaggttcg
    35821 ttcctgttcg ttatctcttt attgatttgc cagaccggca acaagttgcc ttttatcacc
    35881 aaaaagcgac aaggacaagg gtaaatggca cgctgcgcgc ccttgtcctt gtcgcttttc
    35941 gttgatttat tctggccaac aaatcaataa agaaagaact aacgaaagga agtaaaacct
    36001 ttcctaagag gaggcagaat caaacatgcg aaagaaattt gattattggg gtgttccctt
    36061 cagcgaatta ttcccaaact atcatgcacc acatactgtt gaatgcgatt gtggagaaag
    36121 agcgaaatgt atcaaaagct atcgtttgta tcaatgtcca acatgcggga aaaaatatac
    36181 cttgtcttat ggcgattaca ttttgattga tgagaaagga aagaaacgct gatgaaaaca
    36241 caacacaaat tattgactgt aaaagaagtc gttcaaattt tggtggacaa agaagtcatt
    36301 tctgaaaaag tagcagaagc tttacttatt ccgcggtact ggacgataga aaccccttta
    36361 gtagaggatg caccgactat gctatttgaa gacctagtga agattgcagg atatgaactg
    36421 cattatcaag aaaacatgga aacttatgaa atcgaatatt ggttaaccaa attagagaac
    36481 taaaaaggag aaaaaatatg gctcaaaatt actatgacga atttgtcaaa ttacctttgg
    36541 ataaaatggc acaaaaaatg gaggatatga cgttcctata ccatgaaaca cgtgtaccca
    36601 aaaaacatta caaggaaaaa ttgtcagtcg cagtggaaga aatgattgaa tcaggtgtcg
    36661 aaatgaatct cattgcgacg tactatcgaa cactggaaga actgaaaaaa caaaatggaa
    36721 aatggttttt ccaagcctta ctatgcttgg aagtaggtgt caaaccaagc actatcaaac
    36781 cttccgaata tcaagcctta gaactgactt atgcaaaatt cgcacagatt aagaaagcta
    36841 aagcaatggc tagcgactgg ctagatactt ttgaaagtat cgaaaaaaat ggtgcatttt
    36901 atacgattga atcggaggag aaagataatg aataaggtgg tttatcagct aacaacagaa
    36961 gatgtttcgc aatttttaac aagcaaagaa cacttgaatg aagtaatcag acggattcaa
    37021 aaagacttta gttgtatgga agaaatgaag tactttattc aaagagtgga agaacagttg
    37081 tatatggaaa gcgaaacaag tcaaacgaag gtcacagtca gccataatca tttagacgtg
    37141 tataaacgtc gtcctgaaac ggtaaaaaaa ggaacagagc ttctacgact agaaacagaa
    37201 aaatacatca tgaatgtgta cctagaaagg agtgagctgg tcgaaaactt tgtcagctac
    37261 acgatttgta ggaaacacta ttactcagaa tcagaatatt attcaatttt cgagagcatg
    37321 gaattaatgg aagaagaaat ggaagagcta aacagcaaaa atgcgacggt agacgattat
    37381 taccttgtat ttactaggtt tttgacgtgt gggacggaat ctatcgtgtt aatgggaatg
    37441 gattatgaac tcgctgtacc cgaagcattt ttgagggaat atgtagaaga agtagataac
    37501 ccaggcgacg actggtacac gactttaact gattttttag acacctacac ttacgacgat
    37561 acagaagaaa tccaaaatga acttgaccgt aaacgaatgc catatcaacg atttgaaagg
    37621 aaaaaaggta aataaccatt tataccgaaa agtgaggatg tgtgaaataa gaacaaaaaa
    37681 ccttgaaatt aagggaatgg ttcttatttc gcaccaatcg ctccaaccgc ccccgccgct
    37741 taggtggtgc gaaataagcg ggcaaagccc gataacctaa gagaaagaag gagatccata
    37801 tgaataaaat cattcctcta ttgttcgtac tgtttgttgg cgagatactt atgcgtcatg
    37861 ctgcagctgt acatttaaac ttcgtagtat tcttaggaat actggtattt tgtttgatct
    37921 tttggcgatt tgtattagcc cttgtgagtc tactattcaa gatgattgca gccatatttt
    37981 tcatagggtt atttcgatga aaggagagag tactaatgcc aattgcaata ggaaataaac
    38041 ggttacccgt gacattagat gaaaagagac aaaaagaatt gcagcaacta aagcagaagt
    38101 acggaaaaag tgaatctaag attatgtgta ttgcgttaga tctattgatc gcgcaagaaa
    38161 aagcaggatt tgatatacca gcactaaaaa agtgacgtca cttttcattt gaagaaacaa
    38221 aagtgatagc acttaattgt tcctactatt tagaatattt tctatgtcct ttttattcca
    38281 ttttgtatca ataactatga aatctttaaa gtatccagaa gagattattt ttagatcaat
    38341 ttttttctta cctgattcta aagattttgt tttcttaaac atattatgat ttcctttcga
    38401 ctattttcat ataaatgtta acatagttta tggattcatg gaatgttaat ctaatttaat
    38461 agaaaaggat aattaagtat atacgttttt tcttatgtat gttatactat gtatatacag
    38521 aaagaggtgt ctactatgga aatgttacct ataaaaaaat ggggaaatag taatggcctt
    38581 agattgccaa agtatattat ggaatatctt ggaattcata cagaagacaa agtgaagata
    38641 atacaagaag aagtaaacgg tcagaagcgg ctaattatcg aagcggctaa tatagaaaat
    38701 gaattaacaa ttgaacagtt atttgaaaat tacactgatg aaagaaatca tgtgacgatt
    38761 caaaatttag gtgaagcagt aggtaatgaa aaatggtgag aaaacctaga caaggcgata
    38821 ttctattatt gaatacagca cctcgatctg gacacgaaca gacaggtaaa agaccttata
    38881 ttgttttaag tcatgatatt attgcggatt atagtaacgt tgttacagtt gctccaattt
    38941 ctagtacaac tagaaactat ccgctatatg taaatataaa ctcagaatat gaaatgaaga
    39001 caacaggaaa agtattatta gatcaactaa cgacaattga ttatgaagcc agagagtgta
    39061 tttttttgga aaaagcgcat gatagtttag ttgaggagtt attgatgaaa gttagaacag
    39121 tttttcaaaa agtaagcaaa taaaatagaa acagcagcat gatcaactat tcgtgattga
    39181 ccatgctgtc tttttttatt ctatatacgg tttgtctcgt aacattgtag tcttttgcaa
    39241 tcttagcgat cgcaacacct ttttttagat cctcaacgat gtttttataa acaagacgac
    39301 gttggggatc ttttgcattg gcactgtata gcttaggacg tcctttataa acaccgttcg
    39361 cttttgcaat cttaatgcct tgtgcctgac gtcgtttaga ttcggtacgt tcttgttcag
    39421 cgatcatggc caaaatttga atgatcaaat ctttaatgaa tttatccagg agaggattgc
    39481 caattgcttc ggccatgatc ggtagacttg tgatgatcag ctgcacattt ttcttcttca
    39541 agtaattgac tgaagcgatg atctcgtcgt aatttcttcc aagtcgatca atagcttcca
    39601 caataaaaat atcttgatct cggacgaaat ccagtgcttc ttgaaaaaca agtcgatgag
    39661 taatcgttgc gccagattgt ttctcaatga aaattctctc tgcaccgaat tttttcattt
    39721 cttcaatctg ccgttgttca ttttgatcga tggatgagac acgtatgtat gcaactctca
    39781 aaatttttac ctactttctg caactattta aaaaacaccc taatcataca cgagaaaacc
    39841 agatttttca atgtcgcggt cgtgtctagt tagggtatac cttatataga caaaaactaa
    39901 tgatggagga aatagtatgt gtcacttttc tttttaaatg gcaatatttt ttgtaaataa
    39961 atgtcaatca agaaaattta aacgattatg acattaaaaa tatattttat gaattttcta
    40021 gtatataact tgatttatct gttaagattt attaagaaaa cgttttctta tttttgattt
    40081 cgaggtgata gacaatgaaa aataagttta ttttatcttt aggtatttta actattggaa
    40141 ttagtgtaac tggaactaca attcctacat ttgctgatag tacaaatgaa ccaactactg
    40201 tagtagataa taaccaagaa ttaactcctt tagataggaa cggaatagcc attggtaacc
    40261 caactaacga cttaactgtt actgaaaatt atttaatagt ttctccatta cttgctcgag
    40321 cagcttctaa aacgatacga gtgacaggaa cgatgatttt tgataaagtt ggttggcctt
    40381 ctagacctcc tcaatcacgg aattggaaag aaaaaagaaa tggttattgg tactatggaa
    40441 aattgtattt acaaagttat aaatcagctg gaaacaattg gattgcgact tattcaggaa
    40501 ccttaactag aaagtaggga ttttttatgc cagaatttgt gaacacacca aatttttatc
    40561 aagatagcga tattctaagt aaaattaatt taccgaaaga agaatcagct ttacaagttc
    40621 agcgaacttt acaggagaca caaaaaattc aaaaatcaat ttctaatcta gaagattctg
    40681 aagaaaaaag aaaaaaaaga gaccaaaaaa catatcaaaa agaaaaataa tgattttaaa
    40741 aaaagagtgt ctagtcgaca ttcttttttt ctaattgaaa gtgttcataa aatcacccat
    40801 ttctggacac tttctttttt tgctttattt ttcaaaaaaa caattcattt catgttcata
    40861 aacccataac aatatctatg ttcagttaaa ctatatggat aaagttatgt tacaataaaa
    40921 ttaatgaagg agtgattttt ttgaaaatag gatacgcccg ggtatctact ggtttacaaa
    40981 atttggattt acaagaagac agcctaaata aatttggttg tgaaaaaatt tttactgatc
    41041 atatgagtgg ggcaaaaagt aatcgaccag gattagaaat ggccattgat tttgttcgct
    41101 ctggcgatac acttgtcgtt tggcgcttag atcgtttagg tagaaatatg gaagatttga
    41161 tttcaatcgt caatcgactg aatgagcgtg gtgtgagttt tcatagtatt caagaaaata
    41221 ttacgatgga caaatcaagt tctacaggac agctcatgtt tcatttgttt gcggcatttg
    41281 cagaatttga gcgtaatttg atcctggaac gatccgcagc aggacgagca gctgctcgtg
    41341 caagaggacg gtttggtggt cgtcctgaaa aattgtctga acaagatttg gaattactga
    41401 agactttagt ggatagcggt acacctatta agactattgc ggaacggtgg aacgtctcaa
    41461 gaaccaccat ttatcgttat ttggcaaaaa tggaggagaa aaatggaaag acaaattctc
    41521 attagttttt gtcatttaat gctgaaaatt tttgccattt atctagaaaa gtgacaatag
    41581 tatgagaatt ttttgtaagt gtctactgat aacttattct caaatgttgt aaggatttta
    41641 ttcatttgtt aatattaa
//
LOCUS       DAZKNH010000015        36690 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000015 DAZKNH010000000
VERSION     DAZKNH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.015"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..3347
                     /locus_tag="ACG8MG_000755"
     CDS             69..3347
                     /locus_tag="ACG8MG_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFQ0374822.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3888..5795)
                     /locus_tag="ACG8MG_000756"
     CDS             complement(3888..5795)
                     /locus_tag="ACG8MG_000756"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFQ0374823.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5925..6800
                     /locus_tag="ACG8MG_000757"
     CDS             5925..6800
                     /locus_tag="ACG8MG_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFQ0374824.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6880..7854
                     /locus_tag="ACG8MG_000758"
     CDS             6880..7854
                     /locus_tag="ACG8MG_000758"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0374825.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8034..9179
                     /locus_tag="ACG8MG_000759"
     CDS             8034..9179
                     /locus_tag="ACG8MG_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0374826.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9316..9660
                     /locus_tag="ACG8MG_000760"
     CDS             9316..9660
                     /locus_tag="ACG8MG_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFQ0374827.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9931..11217
                     /gene="efmA"
                     /locus_tag="ACG8MG_000761"
     CDS             9931..11217
                     /gene="efmA"
                     /locus_tag="ACG8MG_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFQ0374828.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11355..11954)
                     /locus_tag="ACG8MG_000762"
     CDS             complement(11355..11954)
                     /locus_tag="ACG8MG_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0374829.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12358..13479
                     /gene="mnmA"
                     /locus_tag="ACG8MG_000763"
     CDS             12358..13479
                     /gene="mnmA"
                     /locus_tag="ACG8MG_000763"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFQ0374830.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13725..15146
                     /gene="arcD"
                     /locus_tag="ACG8MG_000764"
     CDS             13725..15146
                     /gene="arcD"
                     /locus_tag="ACG8MG_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFQ0374831.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15160..16278
                     /locus_tag="ACG8MG_000765"
     CDS             15160..16278
                     /locus_tag="ACG8MG_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFQ0374832.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16338..17630)
                     /locus_tag="ACG8MG_000766"
     CDS             complement(16338..17630)
                     /locus_tag="ACG8MG_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0374833.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17780..18340
                     /locus_tag="ACG8MG_000767"
     CDS             17780..18340
                     /locus_tag="ACG8MG_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0374834.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18469..18846
                     /locus_tag="ACG8MG_000768"
     CDS             18469..18846
                     /locus_tag="ACG8MG_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFQ0374835.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18779..20851
                     /locus_tag="ACG8MG_000769"
     CDS             18779..20851
                     /locus_tag="ACG8MG_000769"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0374836.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21062..22009)
                     /locus_tag="ACG8MG_000770"
     CDS             complement(21062..22009)
                     /locus_tag="ACG8MG_000770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0374837.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22255..22719
                     /locus_tag="ACG8MG_000771"
     CDS             22255..22719
                     /locus_tag="ACG8MG_000771"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFQ0374838.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22781..23824)
                     /locus_tag="ACG8MG_000772"
     CDS             complement(22781..23824)
                     /locus_tag="ACG8MG_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFQ0374839.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24201..25166)
                     /locus_tag="ACG8MG_000773"
     CDS             complement(24201..25166)
                     /locus_tag="ACG8MG_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFQ0374840.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25400..26245
                     /locus_tag="ACG8MG_000774"
     CDS             25400..26245
                     /locus_tag="ACG8MG_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0374841.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26436..27623
                     /locus_tag="ACG8MG_000775"
     CDS             26436..27623
                     /locus_tag="ACG8MG_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0374842.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27629..27985
                     /locus_tag="ACG8MG_000776"
     CDS             27629..27985
                     /locus_tag="ACG8MG_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFQ0374843.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            27987..28325
                     /locus_tag="ACG8MG_000777"
     CDS             27987..28325
                     /locus_tag="ACG8MG_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFQ0374844.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28401..28657
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28720..29754
                     /locus_tag="ACG8MG_000778"
     CDS             28720..29754
                     /locus_tag="ACG8MG_000778"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0374845.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29747..30433
                     /locus_tag="ACG8MG_000779"
     CDS             29747..30433
                     /locus_tag="ACG8MG_000779"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0374846.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30457..31305
                     /locus_tag="ACG8MG_000780"
     CDS             30457..31305
                     /locus_tag="ACG8MG_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0374847.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31502..32275
                     /gene="sufC"
                     /locus_tag="ACG8MG_000781"
     CDS             31502..32275
                     /gene="sufC"
                     /locus_tag="ACG8MG_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFQ0374848.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32288..33574
                     /gene="sufD"
                     /locus_tag="ACG8MG_000782"
     CDS             32288..33574
                     /gene="sufD"
                     /locus_tag="ACG8MG_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFQ0374849.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33571..34806
                     /locus_tag="ACG8MG_000783"
     CDS             33571..34806
                     /locus_tag="ACG8MG_000783"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFQ0374850.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34793..35263
                     /gene="sufU"
                     /locus_tag="ACG8MG_000784"
     CDS             34793..35263
                     /gene="sufU"
                     /locus_tag="ACG8MG_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFQ0374851.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35268..36659
                     /gene="sufB"
                     /locus_tag="ACG8MG_000785"
     CDS             35268..36659
                     /gene="sufB"
                     /locus_tag="ACG8MG_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFQ0374852.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT        11994 a         6781 c         7867 g        10048 t
ORIGIN      
        1 tgacagtaaa aataaattag gtaatacttt aagtaaacgc tttctaattt gttacgggag
       61 gaatacactt gttaaaaaaa cgtataaaat acagtgcgct aatgatgaat ctccttatgc
      121 tcagtacccc ggttttaatt tctgtcgttg aagttgctgc taacgaacaa cagtctgtga
      181 acgaagaaaa tgcacacgtg aacattgaag ggatgtcaac aatggaggaa ataccaagaa
      241 ctagtgaaga tatggatacg gaagaaggaa atgatcctat gatgaaagaa tcagaaaatg
      301 aaaagacaaa aaagcaatct gaagaagcaa atgaagagtg gaaaggggtt gttttcggag
      361 aaagcacaag tgctagtagg tatgccattg aaccattaga agacggcgtg cggctctctt
      421 ccacgaataa cggcggaaaa ttccagtcaa gcggatcaga tggaatgacc tattattatc
      481 aagcaatccc caaagacatc aactttagga tgcgggcgac gatcgaaata gaatcttgga
      541 cctatacgaa tgcccaagaa ggatttgctt taatggtaag agacgcagta ccaaaaaatc
      601 atttgtttgg gcaggcattt tattcaaatt catttgccat actaggcagt cggatcgaat
      661 atgtctggga ctcggataga caagaagtgg tcaacacgag cggcagcaaa tatacgatgc
      721 gacttggatt ggggacacgg gcaatcacag gaatatcatc tgaagatccg caagctgcgc
      781 cagctcctgg gagtgtctca gtggaaacaa cacctttaga gacttcagca gggttcttgg
      841 aaaaagaaac aggttcttat aatatattcg gaaacggtca tgggaatctg tttccggcca
      901 cgaactcgat tactaaactg gacgtagaga tgaaaaaaac gaatactggt ttagaggcat
      961 attattacga tcctgaaaca ggagaagaaa tggcttctga catcatgtat gactgggaaa
     1021 aattatacgc ttcagatgaa tcggttattt atgcaggatt tgctgcagca aggaatatga
     1081 cgatcaaggt agaaggaatc gaagttgctt actcagatcc tgcgacagat cctcctgggc
     1141 aagaaagacc tactcgcttg attgatccga tttacactgt gacctctagt aatcattcag
     1201 gaaaccaaga tcatactatg tcctttagag caaatgctga cggattattg acaattaaaa
     1261 ataaagcaac aggagaggtg cttaagaacg caaaaaactt ggctatcact gcgaatcggg
     1321 aatttgatta tcagacaaaa ctgatggaag gaacgaacga atttactttc agttttacgc
     1381 cagataaaaa ctatccgcca gaagaatacg tagagatgac tagttatgaa acaaaagaga
     1441 ttcttcatac agtggactac cgtaaaccta aatattcgac agtttatgta acaccagaag
     1501 ggtcagatgc aggaaacgga agcagacaaa atccattatc tctgacacaa gcattgagat
     1561 acgcatatgc tgggcagaca atcgtcatgg cgcctggaac ttattccttc gaaagaggat
     1621 tgacgattcc aaaaggcgtt aacgggacaa gagaaaaccc gattcagttg attgcagaga
     1681 aaaatccaga aaacaagcgt cctgtacttg attttaaagg aactggaaat gggatgacac
     1741 tgttcagtca ctactggggt ttacgagggt ttgacattac aaatagtgca gctatgcaaa
     1801 aaggattaca gatttctgga aatcataatt tgatcgaaga gattcatgct tatcggaatg
     1861 gaaatacagg aatccaaatc agcggtagct caaatgatcc tttcgaagca tggcccgcgc
     1921 ataacttagt caagaactgt acatcttttg aaaatgccga tccaggattt gaagatgccg
     1981 atggatttgc ggccaaactg acaataggag aaggaaatgt atttgacggt tgtatcgcgt
     2041 atcataatgc agatgacggt tgggaccttt ttgctaaaaa tgaaacaggt tcgatcggaa
     2101 aagtaatcat caaaaactca gttgcttatc gaaacggctg ggtaccagga attgagggag
     2161 aaggtaacgg taatggattc aaaatgggcg gttcttcttt aacgggccct catgaattga
     2221 tcaatagttt gtctttcgaa aatcttgcga aaggaatcga ttccaatagc ggaacagata
     2281 ttatcgtcaa tagcagtaca agttttaata acggttcata taatgtggct ctttatacct
     2341 cttcagcagg aaacacagac taccatgctt ctggtatctt atctttccga acagagaatc
     2401 ttgagatgag agagcagatc cgcccattga atcaaaacga agatcaaatc taccactcgt
     2461 tgaactacta ttggaatgaa cgagagaaac aatcagaaaa tactcttgga cataccatat
     2521 caaaagattg gatcgagtct ctaaatacat gttcgaaaga aggtcagcag cctgttacgc
     2581 gctatgaaaa tggaagtatc catgttcatg gactgatgca gattaaaccg gggaatcgtc
     2641 aaacagaaaa ggaaagagtg gatggtcttc cattatctgt aggtgctatt tttgacggag
     2701 aaacctcacc gtcttcggaa tatccagagt atgcaatcgt acaagaacca aatcagacaa
     2761 aaccaaatga acaaaataat ccgattatta tacaagaagc aaatggtgtg gcggagaatg
     2821 cagaaggaaa tatcattgaa ctgaagcctt ttacagtttt tgtgtcaact ttcacaccaa
     2881 atcaaggaac gcctgtcttt gaagatatgc gttatttccc agaaaagatc caaatcgttg
     2941 aaacacagtc aagtgctcaa ttttctaatc ataccgctat tttttcagca ccttcaaaag
     3001 gagaatatac gctagaagtc acttatcgtt cggaaatatt tgatggggaa aaatggtctg
     3061 ctcatacaga atctgttacc cagcaaaaag cgatcactgt gcgagaaact gccgatgtgc
     3121 ccccgggaaa tgacaaacca gaaagagaaa atccaggaaa tgaatcacca aatggaaaac
     3181 caaatggaaa accttctcat ccggatagga cagcaaaaga aagtgaaaaa aatggcactc
     3241 aaggactccc aaaaacggga gaatttaata acccgttgct agcattagct ggaggaattt
     3301 tgttaatcgg tgtagttgtt tatgtcatga aacagcgaaa aaaataagtg gaatttctcc
     3361 ttttatgatg taaaacaatt gactgaggaa agtaaaaata gaaggaaatg agaccaatag
     3421 atgtctcact tccttctatc tttttataga tgaggttctc cttgctgctt ttgattggct
     3481 gttcttatta cttgatgcag gacagctttt tcacaatctc tccactaaac ggtgttcaat
     3541 cagctgaccc tcggtattag ctcaacaaac ggcaaaatat gaggaactat ttttccgttt
     3601 ttttctctaa tactcaggtc aggacgctga tttcacaacc tctcgataaa cggtgttcaa
     3661 aagccagttt ctcggtagta agtcaaattt atgcaaaaat gtggaaagcc attttcacaa
     3721 aatttttctt actacttgaa actaagcagc tttttcacaa cctctaatac atggtattcc
     3781 agaagtaact tcttcgaaaa ataagccgaa atgtcacaaa aatttgagaa gcgattttcc
     3841 caaattcctt cttatttctt gaagctgacc acttctggca caacctctta ccagattcgc
     3901 actctttttt ctggcgctag atacatagcg tcttgttccg tgataccaaa tgtttcatag
     3961 aattcgtcaa ggttttttgg ttgcacgttt gctcggagtt tagcaggggc atggacatcg
     4021 atttgcagta aaagttgttg gtattctttt tttgcttttg tacgccaaat cgttgcccaa
     4081 ttgacaaaga attcctcgat agaaagatct gattccgttt ttgctgcttc caatgcacag
     4141 cttagaccgc ctgcatcagc aatattttcc gaaacggtta attttccgtt tacttttccg
     4201 tcagcaaaag gcagaccatc gaattcttcg atcattgctt gtgctttgtt ttgaaattgt
     4261 gtaagatctt cttctgtcca ccagttgttc aagttgccat attcgtcaaa taaagcgccg
     4321 ttattatcaa atgcatggga aatctcatga gcgataacag ctccgattcc accataatta
     4381 gcagagcttg attgttctag actgtaaaat ggtgcttgaa ggatcgctgc cggaaagacg
     4441 atcacattcc gaaatggatg ataataagca ttgaccgtgt cagcactcat ttcccattct
     4501 gaacgatcca ctggtttgtt ccatttgctg aaacgatctt ttaatgtgag tttgctgaaa
     4561 acaagagtat ttgaaagcaa agtaccaccc tgatctgcag gtactgtttt gaactggtcg
     4621 aataatgctg ggatcttatc ggggtagcct acttggatac ctaagttatt taattttatg
     4681 acggcttttt cttttgttgc atccccaagc cagtcatttt cttctagacg ttttttataa
     4741 acggcgatca tttttttcac catttgttct acgtcttttt tagcagcttc gccaaaatat
     4801 ttttttccgt agtaatcgcc aactacttga tcgaattgtc cagaggcaag gtaatacgct
     4861 gcttttttct gaggcattgc ttcgtctgtt ccagacaata cgcgagaata gattccgctg
     4921 acttggcgga attcttctga tagataacca ctaagagaac gtactgtttt aatgatcatc
     4981 cagcttttga ataacggaaa atgagcttct gtcaggatgt cattcaaatg atcgaagtac
     5041 actggatcag ttacgatcac tttatctgga gttgtcccaa tcaaacccgt gacaagtttt
     5101 ttcaagtcca gtttatctgt gtatttaata aatgtatcaa aagattgcgg attatacatt
     5161 ttgctgtaat ctgcgttttc ttctgcactt tttacatgag gagcaatcaa tttatcaaat
     5221 tgcactgctt gttccgtgat tgcttctgcc tgactttttt ctttgccggc aagctctaat
     5281 aactgaacag ccatatcaaa gaaaacgcct attagttgtg caccgttggg atggtctgat
     5341 gcataatacg tcttatctgg aaggaataaa gaaggcggat aagcaaagaa ggcgttcgtt
     5401 tgcgcattct tcatatctgc atctacatcc agattaaatg gcaatggtaa tgtatccaac
     5461 agccattctg gaaattgctt attcaattcg tcgaaagact gcaatgattc aactttttcg
     5521 attataggtt gaagcggttg agcatgcaac tcatttcttc ggtcataatc gtttgccagt
     5581 tgataatatg caaggaaatg tttcattttt tctgaagaca ctttttctgg atgggcaagc
     5641 agatcgtctg tttctcccat caaaagttgg tcgatcccgt caactaaatc ttggaaaccg
     5701 cctgtagctg gtttatctgc agggattttt gctgtttttt gccattcttc attgactgct
     5761 tcatagaaat cttgtttcaa tcgttctttg ttcattcatc gatcgctcct tttttgtttc
     5821 gtgtgcccat tatcttacaa aatgaaggaa ttaacaaata gaacataaaa aattgaaaat
     5881 tcgaacaatc aaactgattt gtttttttca atccttcact tataatgaaa aaaaggaggc
     5941 gatcccaact gaatcaagta gtggataaac ctttatattt caaagtagat aaaaaaatta
     6001 aaaaattagc cagcactcag cagctgcagt caagaaaaag taaattgctt tttttagctc
     6061 tggtttttga agatcaaagt tatgtgatta tcgatcaatc aggacatccg gtagaatatt
     6121 ctccagcaaa atatacttat caagagggtc tttcttgtaa aaaatggaag ctgatcaatg
     6181 aagagccaat cgagttatct agatggatca acagaaaaga agatattccc gttttgatcg
     6241 aagaaaaaag gtcaggaaag gaactcgcaa attgctgggt gggattgcca gaagaacgtt
     6301 ttttgaggta taagaaatgg gctaccccct caggctatct ttgtggtact tacgcggcag
     6361 cagttctcct tgcctattat caagactatc gtaaggggtg gatgcttcca aatgagattc
     6421 gcaagaaaaa tacggctgat tcgttggttt tgacgaaggc attgcgtagc cagatccaac
     6481 cactaggact tcctacgatt ccttttcagg ttagtacagg gatttctagt tttttaaaga
     6541 agaatggcaa tcatgagcgt gcacgagcaa ctcttctagg ttcatggcag cgagctacaa
     6601 aacgtatacg tgaaggcaaa cctgtgatga ttggtatttt gaaagtactt ggcagtacat
     6661 atggcaatca ttgggtgaca gcgtacgctt attttgaaac tgaaacagga gaacgttatt
     6721 acaaagtaca cgacaactgg ggcgattatc ataaagtcat tcctgccagt tggagtaacg
     6781 gaacagtttc acttccttaa gaatttgctg aaattaacag aaaatcttgg aaaaatatgt
     6841 taaactatcg aagaatagag aagttttggg gaggactaca tgagtaataa gtatcaggat
     6901 gacacgttga agatttttag tttgaatggc aaccgtccat tagctgaaaa aatcgcgaaa
     6961 gtgttcggaa cagaattagg taaaagcgtc gtgaagcaat ttagtgatgg cgagatttcc
     7021 atcaatatcg aagaaagtat tcgaggggat catgtgtata tcgttcaatc aacgaatgca
     7081 cctgtcaatg attactatat ggaactactg atcatgatcg atgcaatgaa gcgagcaagt
     7141 gcgaaaacga tcaatgtcgt attgccttac tatgggtatg ctaggcaaga ccgcacggcg
     7201 aaacctcatg aaccgatcac tgcgaaattg atcgctaact tgatcgaaga agcaggggca
     7261 actcgagtac tgactttaga tctgcacacg gttcaagtac aaggattctt tgatattcct
     7321 gtagataatt tatttacgat gccgttgttt gctcattatt accgtcaatt aggtctgacg
     7381 ggagatgaca tcgtagttgt ttcgccaaaa aacagcggtg ttcagcgtgc acgtagtctt
     7441 tcagaatatt tgaacagcac attagccatc gtagatcatg cagatgatgc ttcaactgag
     7501 ggtggttatg tgatcggtga tgttcaagga aagacatgta tcatggtaga tgatatcttg
     7561 aacacaggag caacgcttgc acgagcagca aatgtattaa aagaaaacgg cgcaaaagaa
     7621 gtatatgcct gtgcgtcaca tggattattg tccgatcatg cgaaaagtat tttagacaat
     7681 gcaccaatca aagatatttg tatcactgat tctgttttca cagaagaaaa tcgtcatcca
     7741 gaaaacttaa caatcgttac atgttcagaa ttaatgggtg aagcggtcaa acgcatccat
     7801 gaaaatacac cgatgagtcc gttattccgt ctggaagaaa aagaattcga ataaacaaaa
     7861 aaaagaggga aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt
     7921 tgtcacagct ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct
     7981 ttggtttgtt cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg
     8041 aagtgaaaac tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg
     8101 aagcgatgac ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg
     8161 gtcgccacgc gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag
     8221 caaaacctca cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct
     8281 tagaaacagc gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac
     8341 atcctgctgt tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc
     8401 tgcctgtaga tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag
     8461 atacgatctt ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca
     8521 aagaaatcgg tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag
     8581 cgtacggaaa ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg
     8641 ctcataaaat caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata
     8701 tccctgcatt gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt
     8761 tagcagggat ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag
     8821 cacgtaacaa caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg
     8881 aaatccctta tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc
     8941 gcttaattgg cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta
     9001 tttctactgg ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttagaagcaa
     9061 tgtatggaga agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa
     9121 ataccgaaga cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac
     9181 tagtgaagtc attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac
     9241 ttaactgaaa aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt
     9301 atcgaggtga aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc
     9361 tatcgtttgc atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca
     9421 aacggaggca attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga
     9481 ttcaaattaa aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca
     9541 gcaaatggat tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat
     9601 aaattttatt ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga
     9661 tctgtggata taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg
     9721 taaggacgac gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt
     9781 taatattttg ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt
     9841 tagggaaaaa ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta
     9901 tacaaaatga aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg
     9961 aattggaaga gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt
    10021 atggtcgtcc aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt
    10081 ttgagttttg ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga
    10141 ccgctagtgg atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg
    10201 attttcgcgc ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta
    10261 gtttttgtgt ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag
    10321 tcgattatgc cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga
    10381 atggtccaat cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc
    10441 gttcctgtca attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta
    10501 ttgatttttg tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt
    10561 tttaaagatg caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata
    10621 acgatcaatg gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg
    10681 atgacgctag attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac
    10741 gcagtcggaa tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga
    10801 atgaaaccta ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg
    10861 gttccaaacg atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt
    10921 gcaacgccat atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat
    10981 gttttaggtc gtgtcttagg gaacttcaac tctttgatga acctggcagg tccaattgga
    11041 ttattatttg caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc
    11101 ggaatcggga ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat
    11161 gataaagaat tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat
    11221 ggaatggagt tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg
    11281 gcaataagcc tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat
    11341 atagggcagt tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt
    11401 tgttgctcag ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt
    11461 gcgtctacgc ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca
    11521 tgaagattgc ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat
    11581 tgttgatggt cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg
    11641 tcagtaagcc taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc
    11701 cctttgatca agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt
    11761 gctttttcat attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct
    11821 tttattactg cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag
    11881 agccgcggag caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg
    11941 ataaagtaat tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat
    12001 ctgagcaact cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg
    12061 ataagaagca attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga
    12121 aaggctggcc ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg
    12181 attttttggt tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac
    12241 gacttgataa ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa
    12301 agttgcgacc ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg
    12361 acagacaaca gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta
    12421 acagcacttt tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg
    12481 gatgacacgg acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa
    12541 gtagctgctc aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat
    12601 cgtgtatttg aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg
    12661 atgtgcaaca aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc
    12721 gattatgtag ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac
    12781 atgcttcgtg gagtagacaa taataaagat caaacttact tccttagcca attatcacaa
    12841 gaacagcttt caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca
    12901 attgctgaac gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt
    12961 attggtgaaa agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat
    13021 atggtcactc ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt
    13081 ggtcaacgtc aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt
    13141 gtaggaaaag atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca
    13201 ttgtatgcca caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca
    13261 aaagaattca agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt
    13321 cgcttattgg atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact
    13381 cctggacaag cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac
    13441 cacgcatatc aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa
    13501 cagctatggc taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa
    13561 taaaattaat ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc
    13621 aataatatga ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc
    13681 ttttgcaagt gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga
    13741 cgggtattag caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg
    13801 tgtttgggat cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat
    13861 ggataatcgt tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa
    13921 agcgtccaga tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag
    13981 gtggcttcat cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg
    14041 caacgatgct gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg
    14101 ttccttctat tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg
    14161 gagttgaagg agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat
    14221 tcctattttt aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt
    14281 ggggtaacgt atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc
    14341 agattaaagg ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg
    14401 ttttagccaa ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc
    14461 tttgtttatt gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag
    14521 aagagttagc aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa
    14581 attggggagc agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt
    14641 cttggacgat gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga
    14701 aaggttgggg aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa
    14761 tcttgactaa tctattctta ttgactttct tagttactga ttatgcctat caatttgcct
    14821 attctttatg tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc
    14881 agttttcttt aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt
    14941 ttgaattgat gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct
    15001 atcttcctgg tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg
    15061 gaaaagaaaa agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc
    15121 ttgctgtagg ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt
    15181 gtagaagaat ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg
    15241 atcgaagcat tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt
    15301 ttcgaaaaac gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc
    15361 aaacaagaag tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat
    15421 ggagcgactg gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt
    15481 gtttcgatgc ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct
    15541 gttgactttg ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa
    15601 gccttagtca aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc
    15661 acattgctag atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca
    15721 tatgtattga tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc
    15781 gtagatgtct atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca
    15841 ggtattcgta ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat
    15901 cgagactaca cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg
    15961 aagaataaag aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt
    16021 ttaaaacaat gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct
    16081 acttctttca ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta
    16141 gaagaaacag gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga
    16201 ctaggttact gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc
    16261 ttgaaaaaac aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca
    16321 gtgtttttca tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact
    16381 cctgaagctt tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca
    16441 agcaagaagt tgaccaaaac aagaagagaa gtcgaaagaa agacgccgcg ataaccaaat
    16501 gcactggaaa cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat
    16561 tggttataac tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct
    16621 tggacagcag ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat
    16681 tgccaaacat tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca
    16741 atcgcaagta ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct
    16801 atcaaagtgg caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca
    16861 tgtccatgca gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat
    16921 gcttggatga tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct
    16981 ttgaaaatct gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg
    17041 acgaaaaaca gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta
    17101 gggcgatagc caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct
    17161 gtaacagaac cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca
    17221 gttgcgacaa gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc
    17281 aatgcgacta attgatacac actagtcacg caacccatca aagaaatgac gattgccatt
    17341 cctaatgaag cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac
    17401 caaggagaga taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag
    17461 ttcaattgtg acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg
    17521 actaaactaa atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt
    17581 tcccaagttt ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct
    17641 tctttctgaa aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa
    17701 tgaaagtctc ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt
    17761 tatgagagga aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg
    17821 ctttcgagaa ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa
    17881 acgcttggat atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa
    17941 aacagcgcta acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt
    18001 gggaaaaagt ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt
    18061 ttcaaatcaa gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt
    18121 attagatgga atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt
    18181 aacgaaagaa gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct
    18241 ttccttacgt tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta
    18301 tttttggatg atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa
    18361 caattttaag atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg
    18421 aaaagtgact aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta
    18481 attaccatgt tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt
    18541 gaagaagaag aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg
    18601 aacgaaatcc atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt
    18661 tggaatgacg gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag
    18721 ggacttgcgt tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat
    18781 gaaacaacta attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg
    18841 tgctaatcaa aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct
    18901 tggtcattta ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca
    18961 aatggacacg ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg
    19021 acatcaatta aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat
    19081 tgcgacagat gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca
    19141 tttcaataaa ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac
    19201 tggattcaag caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc
    19261 ccgggcaaaa gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta
    19321 cgcggataat ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca
    19381 agaaaagaaa atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg
    19441 aaaagaacaa gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc
    19501 acaaaaactt gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa
    19561 attaaaaacg gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa
    19621 tcaacgctat ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac
    19681 ccataagatc gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag
    19741 tacgatgaaa gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt
    19801 gaaaaacgga gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca
    19861 ccatgcactt ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg
    19921 aaaaatctat caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt
    19981 cagccaaaca aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg
    20041 tgtcgtccaa gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga
    20101 aaaaggaatg agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa
    20161 accattgtca gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc
    20221 tacgcgtgca gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca
    20281 aggactgcaa gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt
    20341 aacaccagat ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa
    20401 acaagcagga accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat
    20461 caaatcaagc aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg
    20521 tggggccaat ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc
    20581 agattgcaac taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg
    20641 tcaccggaaa atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga
    20701 tggaacgaca gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga
    20761 agaacgacgc ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc
    20821 agcagcacta aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt
    20881 ggtcagcttc aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat
    20941 ttcggcttat ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt
    21001 gacaagactt ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat
    21061 attattggtg aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa
    21121 caggacgctg tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac
    21181 ataacaattg gattccacca atgaacagga agatcgagac catggaaggc catcctgatg
    21241 taggatcacc aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga
    21301 cacaagacaa agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga
    21361 taccatcaat cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac
    21421 gttcgcggtt ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt
    21481 ttgaaaatcg gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca
    21541 agcggaaatc acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt
    21601 agaatttttt cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg
    21661 tgccgacaca atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg
    21721 gcggatcttg cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc
    21781 cagcgtacag agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag
    21841 gataagctga ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa
    21901 taaacaaata ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag
    21961 gaacagtttt ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc
    22021 tttcaatcgt atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc
    22081 tttaaaattt gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt
    22141 ttaccatgtt tcctcggttt atcaaacttt taagaacaat ttttattagt tggcaagtag
    22201 ttttggatag agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa
    22261 atacacacac ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta
    22321 tcttttcagc caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa
    22381 gaacagtttg aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata
    22441 aagagaggta cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga
    22501 gaaatccgaa cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg
    22561 attgggcagg caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc
    22621 gggcaaaatg gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa
    22681 cgccaattgt tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat
    22741 tgttccatgc gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa
    22801 taaatagtaa agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc
    22861 gtgtaccttc tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag
    22921 tgatctcaaa ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa
    22981 acaagctgtt gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt
    23041 ttcgtttttt ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga
    23101 atttttcaaa cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca
    23161 tggcgaattc gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag
    23221 attggagtgt accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta
    23281 atccaagaaa agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga
    23341 tctgtccttt ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag
    23401 aaccacaaac agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt
    23461 ttttaccaat caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa
    23521 ttccttttac accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa
    23581 cagagaattc taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt
    23641 taaaaaaagt atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact
    23701 gtggcagata aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac
    23761 cgggtaagat ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt
    23821 tcatttgatc ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact
    23881 aaaccagtat tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt
    23941 ttcgaataaa cggtgttcca gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa
    24001 tttgagaagc aattttcgta aattccttct tatttcttga agctgaccac ttcttttacg
    24061 acctcttggt aaacggtgtt caaaagcttg catctgcggt aataagccca accagtacaa
    24121 aaacatgaac agctgttttt gtttggccgt tcttattact tgatgcagaa cagctttttc
    24181 acaacctatt gaatcgatta ttaagcgatt tttttagaaa caaattttcc atgtttgcta
    24241 tgttctttaa ttaagaaacg tacgtaatga gcaagaacgt agaagacaac tacacttgca
    24301 aacagacatt ccgcaacttt taagaaatcc aatacgctac tatagatacc taaactgttg
    24361 aagtaatgaa cagtagtaaa tatagctgtt gcagagataa caagcgggaa tgtaaaggct
    24421 gcatagcttg gataaaaacg aagcttgaac aaacgtggaa gcatagacag cacaacgaaa
    24481 tacaatactt gagataaagc gaacaatgct acgacaagat aaaaattagg ttcagcaaag
    24541 tttttaagat aacctgttag gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga
    24601 cgtgtcggat gagccatatt tcttacgaaa aagacacgtt tgatgatgat cggcaataag
    24661 aacgcatagc agatcaatga aatccaaaag aaaatttttc caataagtgg gaagaagttc
    24721 ccggcagtta cagtgatgac cccaaatcca acgtaaatga taaaccagct tggataaatt
    24781 gcttcaattg aaagatcagc tttgataaca tggtgataat tgaaataaaa gacaaggatc
    24841 acttggaaga taacagcgca cagccaaatg aatttgacaa ttggtgcaat actttcatat
    24901 gaaacaaaat aagtacaaat aaccatgagt gacatcgtaa aagttggtga aacggaggcg
    24961 atgattggat catgcaacgt ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa
    25021 atgaatatca taagaatcat agagataccg ccagttagat tgcctaaagc aacatggtga
    25081 tagtctttaa acagatttcc aagggaagct aacccaagga tcaacccgca gatcgggatc
    25141 ggaataattt tcaaaaagtt cttcatatat aatattctcc tttttattta atagatttta
    25201 gtaccaagat gaacgaatac attgtaaaat gaataggaat gtatgattac gagcaagcag
    25261 acagctgatt gtcttcatct aagttactag ttggatcagc aactgttttg ttgtacattt
    25321 ctattataga tggttttttg tatatttaaa tcaaatagtt tttatagtaa ttatcataaa
    25381 aagttatgga agtggtgcca tgtttaagtt attaacgact tttcgagcag tatatgaaac
    25441 aagaaatttt tccagagcag cagaagtact gtttttatct caaccagcag tctcgaatca
    25501 aatcaaacag ttggagcggg aattgaatat cgatcttttt caacgtaacg gaagaaaaga
    25561 gatgcttcca actaagcaag ccgacttatt atatgaacgg acactggttt tgttagaaga
    25621 atgggaagaa actcagcaaa aattgctcaa tgaacaaaat gaagaagaag tatgtcggat
    25681 cgttgcttcc cacacctttg ctgtctatct tttgcctcag ttgatgaaac atctgatcaa
    25741 aaggtttcct aaagtgcatt ttgagatcga aatcacgaat tctcatgaat cattagaaca
    25801 agtagcgaaa catgaatccg attttggttt tatcgaaaag ccattagaga cttccggcgt
    25861 acaaagatac tctttgatgg aagaccagct agtcatcgct ggggacccaa aaagcaaatt
    25921 gtggcttgta agagaagcaa actctggtgt ttttcattac acgcagcgtt attttgaaga
    25981 gatgaatatc caagataaga agatgatcgt gaaaagtaac gatgtaattg ttgcgttgct
    26041 gaaggaagga atcggtcgct cgattttgtc taagcgctct gttccagaaa atgtgccaat
    26101 gactccgttg aatttccatc gatattttta tttcatccaa cgaaccaatc tggtttctga
    26161 tcagctgcta gagattgccg aagagatcct tcgatacaat gaaagcacag aaacttctca
    26221 cagcgaggag aatctcagct ggtagataat tgcatgtatc aaaaaaagtt ctatgataca
    26281 ctaaagataa gcagtaggct tttgtactgc atttgctcaa gcaactgcac aaacggaaag
    26341 ttaatttttc ccaaatgtct atggaaagac tttccattat ctaactggac cgctataata
    26401 atcagcagta tggtttttaa gaaaagggat gaaacatgga aaaaaaatta aaagcttcga
    26461 acgataatcg tattgattat ggcgtgattt taccagtctt tctgttatgt ttgattggtc
    26521 tgctctcatt atatgtagca cttagccatg atccgaacaa tccagcagtc ttgaaaggcg
    26581 tagggatgca agttgtctgg tatttagttg gagcggcagc aatcgttgtc atcatgcatt
    26641 tgaattccaa atggatctgg aaactgactc cttaccttta tggtttgggg ttagtggtaa
    26701 tgggactctt gctcaaattc tatgatcaga atttagctgc ttcaacaggg tctaaaaact
    26761 ggtttcgatt cggtgcgttt actttccagc cagcagagtt gatgaagatt gcgtacatct
    26821 tgatgatggc actgatcgta actaaacata atacgcaagt aaaagaacgg acgatgaaat
    26881 cggacttttg gctgattggg aagctcttga tcgtgacaat accggtttta gcattgatca
    26941 tggcccaaga tgactttgga acgatgcttg tctttcttgc gatctttggc gggattttcc
    27001 tgatgtctgg aatctcttgg cgcatcatcg cacctgtagt aatcttggca gtagtcgttg
    27061 gagcaggaac gatcttcttg gttacgactg aaggcggaag agacttgctt tataaagttg
    27121 gattcaaatc ttatcagttt gcacgtattg attcatggct ggaccctttc catgatacgt
    27181 caggaatgag ttatcagccg gcccaaggac ttttggcaat tggtaccggt ggcttgtttg
    27241 gtaaaggatt caacgtaagc aatatctatg tgcctgttcg tgagtccgat atgattttta
    27301 ccgttatcgg tgaaaacttc ggctttatcg gtggtgcatt cgtcattttc ttgtatttta
    27361 ttttaatcta tcgtatgatt cgggtgtgtt tcgatacgaa taatgaattt tacgcctata
    27421 tcgcaagcgg tttgatcatg atgctgctgt tccatgtctt tgagaatatt ggagcaaata
    27481 tcggattact tcctttaaca gggatcccat tgcctttcat tagtcaggga ggatcctcga
    27541 ttttagggaa catgatcggt atcgggttga tattatcgat gagataccaa aatgaagcac
    27601 ctgcaagatc ttcaaggagg taagaaaatt gtacaccttt tactggtacc cgaaatgttc
    27661 aacatgcaaa aaagccaaag cgtggttaga ccagcatggc gttagttatc aaaccgttga
    27721 tatgatcaaa gaagtaccga aggcctcaac gattgagact tggctagaaa cgagtcaagt
    27781 accaatccgt cgcctgttca acacaagcgg gatgaaatat cgggaacttg gactgaaaga
    27841 tcaagtaggc gagtttacgg cttcaaaagc gagccaagtc ttatcgacag atggtatgct
    27901 gatcaagcga ccactgatct tacgagagga ccagcttgtt gcaattggat tcaatgaaaa
    27961 aatttatgaa ggagaactga tataaaatgg caggcaagca tttgaagaaa aaggacaacc
    28021 tttggatctt atttaatgga aaagaatact gtgttggttt gacaaatgaa gcgcaagaag
    28081 aacttggaga catcacgttt gccaatctgc caaaagcagg acaatcgcta aaaaaagatg
    28141 aacctctgat cgaagttgaa gcggaaaaag cggtcaatga atttgccagc ccgctaacgg
    28201 gtacagtttc ttctgttaat gataaaatcg gtgaagatat caatatcttg aatgattcag
    28261 atgaaatgaa tgcgtggatc ttaagtttca aagatgttga tccaaaagaa tttgacgaat
    28321 tataaaatca gaaaatcact tgaaaaaagt tgacactgat tcttccagtt gctataatca
    28381 taagcaatcc tatatgaaac gagaaagctt tgaaaagaag agtacatgtc acggacgttt
    28441 gaagagagcc ctgtttgctg aaaatgggta acgaaaatga gatggaagat ggtcttagag
    28501 cagagtaagc gagcgtaagc aaacttacta cgggagtgcc cgttacagcg cgacagtata
    28561 cggaaagaag ctaagtccaa gtactggaag aggctgttat tgcgaagtaa cggtaaatca
    28621 aggtggtaac acggaagttc aagctttcgt ccttttgctt aaggcaagag ggcgaaggct
    28681 tttttgtttg aaaaattata agagaaagat gtgaatggca tgtcattaat ccaactaacg
    28741 aatgtcaaaa agacattcaa tgggaaaaac ggaaacatcc aagccgtcaa tgacgtttca
    28801 ctaaacgttg aaaaagggga tatttatgga atcgtcggct attctggcgc aggaaaaagt
    28861 acattagtcc gtctcctgaa tggacttgag cttcccacga gcggcgaagt gatcgtaaac
    28921 gatcaagata ttacaaaatt aaaaaacaaa gaattacgta cattccgaaa gaaaatcggt
    28981 atgatcttcc agcatttcaa cctgttatgg tcaagaactg tacttgaaaa tatccagctg
    29041 ccgctggaat tagctggcgt accaaagagc aaacgtaaag aacgggcaga agaactgttg
    29101 cgtttagtcg ggttagaagg acgcggacaa gcatatcctt cacaactttc cggcggacag
    29161 aaacaacgcg tcggtatcgc acgagcattg gcaaatgatc ctgagatcct gctttgtgat
    29221 gaggctacta gcgcattgga cccgcaaaca acagaagaag tattagactt attgttagcg
    29281 atcaataaga aattgaattt gaccatcgtc ttgatcacac atgaaatgaa tgtgatccgc
    29341 aaaatctgca acaaagtcgc tgtgatggag ttggggaaag tcgtagaaga aggagacgta
    29401 ttgactgtct tcagacaacc gaaaaaagaa gtaaccaaac gctttgtaca acaagacatc
    29461 gaaccagaag ctgattcgac agaactatta gctgaactca tcaaagaaaa tccaagcggc
    29521 cgtttggcaa ctttgacctt tagcgaaaga aatgccaacg agccagtcat ttcccaagcg
    29581 attcgacgct ataacatcga tgtcaacgtt gtgtatggaa agatcaaaca agtgaaagaa
    29641 ggttcctttg gttcattgac tgtcttgatg actggtgaaa ccaaagaatt agatcaagca
    29701 gaagtgttca tcaaagaaca aggagtagga attgaggtga tccatcgtgg ataaaagctt
    29761 ttcagaaaca tatctagatt ttaaacaaat caacccggaa acgatgaatc aggctatcct
    29821 tgatacacta tatatgacat taatctcaat ggtcttagtg acgatcatcg gattagtttt
    29881 gggattgatc ctttactcta ttggacgcaa aaacaaaccg tatgctcgaa tcatttatgg
    29941 aatcgtatcg atcatcagta atattttccg gtcaacgcca tttatgatcc tgatggtttt
    30001 gatcattcca tttactaaag caatcgtagg cactatgcta ggtgcgcaag cagccattcc
    30061 agctcttgtt ttatccgcag caccatttta tgcccgtcta gtagagattg cttttcgtga
    30121 agtcagccct ggtgtactgg aagcagcaga tgcaatggga gcaagttact gggaaatcat
    30181 ctggaaggtc ttgatacctg aaagtattcc cgcattgatt tctggattaa cagttacaac
    30241 gatctcgatg atcggattta ctgcgatggc cggcgcgatc ggcgcaggtg gacttggagg
    30301 tcttgcttgg caggaaggct accagcgtgg taatctaaca gtgacattcg ttgcgacttt
    30361 aattatttta ctaatcgtat ttatcgttca aggaatcggt gatttcttaa ctaagaaaac
    30421 agacaaaaga taataggata tagggggatg taggatatga aaaagaaaat ttttggattt
    30481 gcagcagcgc ttttgctgac tgtaggatta gcagcatgcg gtaacaacag cgacagcaaa
    30541 gattcagcaa ataaagcaga cgacacgaca ttgaaagtcg gcgcttcacc gacaccgcac
    30601 gcagagattt tggaacatgt gaaaccatta ttgaaagatg aaggaatcga tcttgaaatc
    30661 gtcaaattcg atgactatgt attaccgaac caatctttgg aagaaggcga cattgatgcc
    30721 aattacttcc agcatattcc ttatttgaac aaagaaatca aagaaaaagg atatgacttt
    30781 gtcaatgccg gtgctgtcca catcgaacca atgggcttgt actctaaaaa agtcaaagat
    30841 gtttccgaat taaaagatgg tgcaacaatc attacttcaa attctgaatc agactgggga
    30901 cgcatcatca caattctaca agatgctggc ttagtcaaag tcaaagatgg agtagatctg
    30961 gaaacagcaa cattcgatga tattgaagaa aaccctaaga acttgaagtt caaccatacg
    31021 atcgacccat cacttttagc ttctacgtac caaaatgatg aaggtgacct agttgcaatc
    31081 aacgcaaact tcgcttacgg cgcagggttg aatccattag aagatgccgt attgcttgaa
    31141 aaggacaact ctccttacgt gaatattatt gcgactcgta aaggtgatga agacagtgac
    31201 aaaatcaaaa aactgattga agtgcttcac cgcaaagaag tccaagaatg gatcacagac
    31261 aaatggggcg gctctgttaa accagtaaag gctgatgcaa aataaggtat ggaacaaaaa
    31321 agatgagaaa taagtctgta atggcttatt tctcatcttt tttataccta ttctaattta
    31381 gaaatcaaaa ttgtttaata ataaataaaa gatatttgat tttagtaagg aaggtcattg
    31441 cgcttcattc tcaattagtc taaaatgagt gagaacgaaa gagaatttgg gggtataatg
    31501 catgtctgtc ttagaaataa aaaatctaca tgtgtcgatc gaggaaaaag aaatccttaa
    31561 aggcgtaaac ctaacaatga aaaccggaga aatccatgcc atcatgggac ctaacggcac
    31621 aggaaaatct accttatctg ccgctatcat gggcaatcca aactatgaag taacagaagg
    31681 cgaaattttg tttgacggga aaaatgtttt agacttggaa gtagatgaac gtgcgcgact
    31741 tggcttattt ttagctatgc aatatccaag tgagatacca ggtatcacga atgcggaatt
    31801 catgcgtgca gcgatcaatg cgaaacgtga tgaaaacgac aaaatttctg ttatggcatt
    31861 tttgaaaaag ttagatgaaa aaatggcttt actaaatatg ccagaagaaa tggctgaacg
    31921 ttatctgaac gaaggttttt ctggcggaga aaagaaacgg aatgagatcc tgcaattgtt
    31981 gatgttagaa cctacatttg caattttaga cgaaatcgat tcaggtctgg atatcgatgc
    32041 tttgaaagtc gttgctaaag gagtaaatga aatgcgcgga gacaatttcg gtgcattgat
    32101 catcacccac tatcaacgat tattgaacta tatcacacct gatgtcgttc acatcatgat
    32161 ggaaggccga gtagtcatga cagggaatgc tgacctagct aaacgtctgg aagcagaagg
    32221 ctacgctggt atcagtaaag aattaggaat cgactataaa gaagaagaag cgtaaggggg
    32281 agcaagaatg aaagaaaaaa actggctcga ttatcttgac gcggtaaatg atttttcttt
    32341 aagtaaaggc gaacctgatt ggatgagaac cttccgtcaa gacgcactag cgaaagcaga
    32401 tgaactgcca ttgccgcata tcgatcgggt gaaattccat cgctggtcgt tatttgatgt
    32461 gaaagaaaca caaaccatca gtgaaacagg cacgattccg gcatttgatg caatgaaaga
    32521 caatcctgtg cttgtccaac aaggttcatg gacgattttt gaacaactgc ctgtggaatt
    32581 agcagaaaaa ggagtcattt ttaccgactt gttcactgca atgatcgaat atccagaact
    32641 agtccaagaa tattatatga aaaaagcagt aaacatgaac gaagaccaat tgactgcact
    32701 ccatgtagct tttatgaaca gcggtatttt cctgtatgtg cctaaaaatg tcgtgatcga
    32761 tgaaccactc gagtccttat tcattcaaga tggcgcatcc gatgagcact tcttcaagca
    32821 tgtgttgatc gtggcagatg aacatagtga attttcttat ctggaacgtt tccagacaac
    32881 caaagaacaa gtagcaaaat catcaggtaa tatcatcgtg gaagtcatcg ctaaagctgg
    32941 ttcaaaaatc aaatattcag ccgtagatca attaggagaa aatattactt cttatatgaa
    33001 tcgccgtggg cacattttac gagatgcttc tgtcgactgg gcaattggtg tcatgaatga
    33061 cggtcatgtg atcgctgact ttgactcaga tttagcagga gaaggtgcac acgcagaagt
    33121 caaaatcgtg gcaatcagct cgggcagaca aatccaagga atcgatacac gagtaaccaa
    33181 taaagcccct catacgatcg ggcatatttt gcaacacgga gtgatccgag aaaaaggaac
    33241 attgaccttt aatggtatcg gccacatctt gaaaggggca aaaggtgcag atgcccaaca
    33301 agaaagtcgt gtcttgatgc tttctgataa agcacgtggt gatgcgaatc ctattctttt
    33361 gatcgatgaa aacgaagtaa ctgctggaca tgctgccagt gtaggacgag ttgatccaga
    33421 agaaatgtac tatctgatga gtagaggatt gcataaagaa gaagcagagc gcttagtgat
    33481 tcgaggattc ctcggttctg tcttaacagc aatccctgta gaacaagttc ggaaagaatt
    33541 agtcgaagta atcgaaggga agttgaatgg atgatcgatg ctaaaaagct acgcaatgat
    33601 tttcctatct tatttcaaga cgtcaatgat gaacctttag tttatctgga taatgcagcg
    33661 acgacccaaa agccaaaaca agtactccag gcattagcgt actattacga acacgataac
    33721 gccaatgttc atcgcggtgt ccatacgtta gcagaacgcg cgacccatga ttatgaacaa
    33781 gcaagagaaa aagtacgggc gttcatccat gcaaaagaaa cagcagaagt gctgtttact
    33841 cgtggtacga cgacaagctt gaattgggta gccagaacct tcggcgaccg ctatatcaaa
    33901 gcaggagacg agatagtgct ctcttacatg gaacatcatt ctaatgttat cccgtggcag
    33961 cagttggcaa aacgaacagg tgctgtcttg aaatatatcg aactaacaga agaaggcttc
    34021 ctggatatgg aggatgcagc gaaaaagatt acggacaaaa ccaaaatcgt ctccattgct
    34081 catgtatcca atgtcttagg tgtcttcaat cctgttcaag aattagctga acttgtccat
    34141 gaaaacggcg gggtcatcgt agtagatggt gctcaatctg ccccgcacat ggccattgat
    34201 gtccaagcac tagactgtga tttctttgct ttcagcggac acaaaatgtg cggaccaaca
    34261 ggtattggtg ttttatatgg taaacgaaaa tggttagaag aaatggaacc tgttgaattt
    34321 ggtggcgaaa tgatcgattt tgtcgaatta tacgacagta catggaaaga actaccttgg
    34381 aaattcgaag cagggacacc aaatatcgcc ggcgctatcg cattaggcca tgcaatcgat
    34441 tatttgcaaa aactcggtat ggacaatatc catcgatatg aagaagaact agccgcttat
    34501 gtgttgccaa aattacaagc aattgatgga ttgactgttt atggtccaca agacccgaat
    34561 tttcggacag gagtcgttgc attcaatttg gatgggttac atccccatga tgtcgcgaca
    34621 gctttagaca tggaaggggt agccgtcaga gcaggtcatc attgtgcgca accgctattg
    34681 aagtaccttg aagtatcagc gactgctcgc gcgagctttt atttctacaa cacaacgcaa
    34741 gacgccgacc gtttagttga agcaatcatg gcaacaaagg agtttttcca acatgggact
    34801 atctaaatta gacaatttat accgtcaagt gatcttggat cattcttccc atccgcatca
    34861 tcgtggaagc ttgactgact cgacaagcaa gatcgaattg aacaacccca catgtggaga
    34921 tgtgatcgaa ttgcaagtcg atgtaaaaga cggaatcatc aaaaatatcg cctttgacgg
    34981 cagtggctgc tccatcagta cggcaagtgc ctcgatgatg acagacgccg tcatcggaaa
    35041 atcaatagaa gaagcagaac atttaactgt agactttcta cagcttgtcc aaggaaaaga
    35101 agtcaaagat cccgaccaat taggcgatgc tgctatgctt ggtggtgtag ctaaatttcc
    35161 cgctcggatc aaatgtgcga cacttgcctg gaaagcacta ggaaaagcaa tggaaaacga
    35221 tggtaccgca gctttccatc attaccacaa ggaagaggag tgagaacatg ggcgtaccag
    35281 aattagaaga atataaattc gggtttcacg atgatgtcga accggttttc agtacaggag
    35341 aaggcttgac agaagaagtg atccgtgaga tgtcaagaat caaaggcgaa ccagaatgga
    35401 tgcttgaatt ccgtctgaaa tctctagaaa catttaacaa aatgccaatg caaacatggg
    35461 gccccgacct ttcagacatc gatttcgatg ccatcaaata ttaccaaaaa ccaagtgaca
    35521 aacccgctcg tgactgggaa gacgtaccag aaaaaatcaa agatacattt gaaaagattg
    35581 gtataccaga agctgaacga gcttatctag ccggtgcttc cgcacaatat gaatcagaag
    35641 tcgtgtacca taatatgaaa gatgaattcg aaaagttagg aatcgtcttt actgacactg
    35701 actctgcttt gaaagaatac cctgaattat tcaaagagta cttttcaaaa cttgtaccgc
    35761 caaccgacaa caaattagca gcgctgaatt cagccgtatg gtcaggtggg acctttattt
    35821 acgtgccaaa aggcgtcaaa gtcgatgttc cattacaaac gtacttccgg atcaatgcgg
    35881 aaaacacggg gcaattcgaa cgtacgctga tcattgttga cgaaggagca agcatccact
    35941 atgtcgaagg ttgtacggca cctacttatt caagtaacag cctgcatgcc gccatcgtag
    36001 aaatctttac aagaaaagat gcgtattgcc gttacacaac gatccaaaac tggtcagaca
    36061 atgtgtacaa cttagtaacc aaacgagcaa aagcatacga aggcgcaacc gttgaatgga
    36121 tcgacggaaa cctaggtgca aaaacaacga tgaaataccc aagtgtttac ttagatggaa
    36181 aaggtgcacg aggcaccatg ctctccatcg cctttgcagg agcaaaccaa atccaagata
    36241 cgggtgcaaa aatgatccac aatgcaccaa atacatctag ctctatcgtc tctaaatcaa
    36301 tttcaaaaga cggcggagaa gtaaactatc gaggacaagt aacctttgcc aaaaacagtg
    36361 cgggttcgat ctctcatatc gaatgtgaca cgatcatcat ggacgaatgg tcgaaatccg
    36421 atacgatccc attcaatgag atccacaaca gccaagtctc tttagaacac gaagccaaag
    36481 tctcaaaaat ctcagaagaa caactctact atctgatgag ccgaggcctg accgaatctg
    36541 aagcaactga aatgatcgtc atgggctttg ttgaaccatt caccaaagaa cttccaatgg
    36601 aatatgcagt cgaactcaat cgattgatca gttatgagat ggaaggcagt gttgggtaaa
    36661 cctgttatag gagctagaac ccttgatata
//
LOCUS       DAZKNH010000016        36511 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000016 DAZKNH010000000
VERSION     DAZKNH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.016"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            315..635
                     /locus_tag="ACG8MG_000786"
     CDS             315..635
                     /locus_tag="ACG8MG_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFQ0374853.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            789..1694
                     /locus_tag="ACG8MG_000787"
     CDS             789..1694
                     /locus_tag="ACG8MG_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0374854.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1691..2464
                     /locus_tag="ACG8MG_000788"
     CDS             1691..2464
                     /locus_tag="ACG8MG_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0374855.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2645..4069)
                     /locus_tag="ACG8MG_000789"
     CDS             complement(2645..4069)
                     /locus_tag="ACG8MG_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0374856.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4280..4726
                     /gene="nrdI"
                     /locus_tag="ACG8MG_000790"
     CDS             4280..4726
                     /gene="nrdI"
                     /locus_tag="ACG8MG_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0374857.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4791..6773)
                     /locus_tag="ACG8MG_000791"
     CDS             complement(4791..6773)
                     /locus_tag="ACG8MG_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0374858.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6787..7683)
                     /gene="murQ"
                     /locus_tag="ACG8MG_000792"
     CDS             complement(6787..7683)
                     /gene="murQ"
                     /locus_tag="ACG8MG_000792"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0374859.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7686..8747)
                     /locus_tag="ACG8MG_000793"
     CDS             complement(7686..8747)
                     /locus_tag="ACG8MG_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0374860.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8958..9485)
                     /locus_tag="ACG8MG_000794"
     CDS             complement(8958..9485)
                     /locus_tag="ACG8MG_000794"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0374861.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9552..11054)
                     /gene="eat(A)"
                     /locus_tag="ACG8MG_000795"
     CDS             complement(9552..11054)
                     /gene="eat(A)"
                     /locus_tag="ACG8MG_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0374862.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11312..12433)
                     /locus_tag="ACG8MG_000796"
     CDS             complement(11312..12433)
                     /locus_tag="ACG8MG_000796"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0374863.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12559..13500
                     /locus_tag="ACG8MG_000797"
     CDS             12559..13500
                     /locus_tag="ACG8MG_000797"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0374864.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13650..14546)
                     /locus_tag="ACG8MG_000798"
     CDS             complement(13650..14546)
                     /locus_tag="ACG8MG_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0374865.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14815..16155
                     /locus_tag="ACG8MG_000799"
     CDS             14815..16155
                     /locus_tag="ACG8MG_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0374866.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16373..16816
                     /locus_tag="ACG8MG_000800"
     CDS             16373..16816
                     /locus_tag="ACG8MG_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0374867.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17320..18738)
                     /gene="nhaC"
                     /locus_tag="ACG8MG_000801"
     CDS             complement(17320..18738)
                     /gene="nhaC"
                     /locus_tag="ACG8MG_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0374868.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19141..20364)
                     /locus_tag="ACG8MG_000802"
     CDS             complement(19141..20364)
                     /locus_tag="ACG8MG_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0374869.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20395..21234)
                     /locus_tag="ACG8MG_000803"
     CDS             complement(20395..21234)
                     /locus_tag="ACG8MG_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0374870.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21251..21931)
                     /locus_tag="ACG8MG_000804"
     CDS             complement(21251..21931)
                     /locus_tag="ACG8MG_000804"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0374871.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21933..23006)
                     /locus_tag="ACG8MG_000805"
     CDS             complement(21933..23006)
                     /locus_tag="ACG8MG_000805"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0374872.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23062..23300)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23343..23576)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23653..24324)
                     /locus_tag="ACG8MG_000806"
     CDS             complement(23653..24324)
                     /locus_tag="ACG8MG_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0374873.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24411..27059)
                     /gene="mgtA"
                     /locus_tag="ACG8MG_000807"
     CDS             complement(24411..27059)
                     /gene="mgtA"
                     /locus_tag="ACG8MG_000807"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFQ0374874.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27401..27575)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27748..28185
                     /gene="ndk"
                     /locus_tag="ACG8MG_000808"
     CDS             27748..28185
                     /gene="ndk"
                     /locus_tag="ACG8MG_000808"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFQ0374875.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28207..29073)
                     /locus_tag="ACG8MG_000809"
     CDS             complement(28207..29073)
                     /locus_tag="ACG8MG_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0374876.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29233..31326)
                     /locus_tag="ACG8MG_000810"
     CDS             complement(29233..31326)
                     /locus_tag="ACG8MG_000810"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0374877.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32075..32698)
                     /locus_tag="ACG8MG_000811"
     CDS             complement(32075..32698)
                     /locus_tag="ACG8MG_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374878.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32857..34254)
                     /locus_tag="ACG8MG_000812"
     CDS             complement(32857..34254)
                     /locus_tag="ACG8MG_000812"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0374879.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34286..35257)
                     /locus_tag="ACG8MG_000813"
     CDS             complement(34286..35257)
                     /locus_tag="ACG8MG_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0374880.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35631..36056
                     /locus_tag="ACG8MG_000814"
     CDS             35631..36056
                     /locus_tag="ACG8MG_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374881.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT        10568 a         7607 c         6397 g        11939 t
ORIGIN      
        1 aatttattgc ccgataatat atagaaataa ggtctagtta ctgttttttt taaatgatta
       61 ataaataaca acttctttct atgaaattgc aaagcattca tcttcttttc cgctaacgat
      121 atatccgtca gtgcttggta cactttacta aaaagtatga gattgccgat atctggatag
      181 gtgtgctata ctacagaaaa agatatacaa tctgccaccg ggtaggataa agaggatgat
      241 cattccatta ggtctttgaa gggatgattt tcctcttttt gtgtattttt ttatagttaa
      301 ggaggaatgt aagtatggca tggatggaat taatcatagc aggaattttg gaagtattct
      361 ggtcaacgat gatgaaatgg agtgatggtt tttctaaaat caattattct ttttacacag
      421 tgattggcat gattgctagt ttttactttt tgtcaaaggc aatcaaaagt ttgccgatga
      481 gtctctctta tcctatctgg acagggattg gcgcagttgg ttcggtcata attggtgtgg
      541 tcttgtttca tgataagctg aatctttcca cttggttctt tgtggggatg ctactcatca
      601 gtatcattgg catcaagatt actagcggcc actagacact tggaataagt actgatcaga
      661 aaaaataagc ctttttattt aatattcaat ttttttatga aaaaataaga aataagtcca
      721 atgacctagc gacttctcca ctcatcaggt gtatgcttgt actatctata gaaaaggagt
      781 gaatattttt gaaaagtctt tcggtccaag aattatccaa gaactttggt cacaaacaag
      841 ccctcagcga tgtcagtttt agcttatccc ctggcaaaat cgttggatta gtaggtccta
      901 atggcgctgg aaaaacaacg atcatgaaag ctattttagg actgatccat tactcagatg
      961 gaacaatctg tattggtgaa aatgagattt ctccctctag tcataaaggg ttggaacaag
     1021 taggagcatt gatcgaatac ccgggtatct atccattctt aacaggctat gatcatctga
     1081 agttattctc tgaaaccaac gatgtatctg ctatcgacac tatcgtgaat cagctcaaga
     1141 tggaaaagta cattcacaaa aaagcgaaat cctactcgtt gggaatgaaa caaaaattgg
     1201 gaattgctct tgccttactg aaccatccgt ctatcgttat tttagatgag ccgatgaacg
     1261 gtttggatcc acaagcaaca agagatgtgc gaaaaatcat taccgacttg gctactcagg
     1321 gcacctcttt cttgatctcc agtcacttac tcagcgagct tgaaaaaatg gtcgatgagc
     1381 tgatcctgat caatcaagga aagatcatca aacaatgtac aatggctgaa ttgaaccaaa
     1441 cagcgcaaga tttcatcgtt atctctacaa caaatgatct agctgctcga acgatcttga
     1501 cagaagctgg atatactttg gaagatcctg ataaaatcaa gcttttaaaa gaaaacgaag
     1561 aattattagg aaccgtgatc cagctattgt ctgcacaaaa tatccatgtg acagacgtcc
     1621 agcatctgac gaatgatttg gaatcttctc tattggaaat tctggattca gtaaaggggg
     1681 aagaagaaaa atgacattgc ttgtaaaaca agaattattt aaattgatca agaaaaagtc
     1741 aactgctgtt ttatctgttc tattagttgt tttgctcata ggaacagcac ttctagccaa
     1801 aaaatacacg acgatcattg atccagttga aatgactgct cagcttttta gtgccacatc
     1861 ttggattgtc tttatcatga ttgcagctgc aagcacgatc atttcgatgg aagcacaata
     1921 tggtacattg aaaaatctcc tttatcgaaa gtattctcga ggagaaatat tagtaagcaa
     1981 atggatcaca ttagttattt attctgttta tttatacctt ttagctatta ttgtcactgt
     2041 gttgatgaaa ctgattcttt tcccttcgat ttccttcacg gagaaggttt ctactggtca
     2101 aacactgatc caaagcttgt tcacttatac tttgggtagt tatatcggtt tatggctgat
     2161 tttaagttta gtgttgatga ttgcttgctt tatcaatagt tccggcgcat ccatctcagc
     2221 aggaatcgtc ttttattttg cttcgtcaat catatctggt atccttattg cactcatcca
     2281 aaaatgggag tggatcaaat ggaatccgat cagtatgttg aatctgcaaa accaaattgg
     2341 aaacgaagag atcatgaagc aattgactca tctttctact aatcaaatgc tattcggtaa
     2401 tttagcttac atcgttttgt tcttagcatt aggatatctc gtatttaagc gaaaaaatgt
     2461 atgatgactc atcaaaaaag aggctgtgaa agagacgttc agccttattg ccgattggct
     2521 gtctcttaaa caccgtttat tctgaaaaag gagctgtgac aacatttgtg tcacagctcc
     2581 tttcctagta tgtttttgaa taaacgacag gctcacaacc tctctttata cttcttttat
     2641 acttctatac gtgaagcatc ccagtggcaa ataaaaccat tgcaatcact ccgaccatcg
     2701 tcacacctat aagtaaaatc ttatctaagt ggatatgttg tacaggctcg ttttgctcct
     2761 tacgtgcttt gaggtaaaaa taaatgcctg gcaagaaaag aacaagaaca agcattactt
     2821 cattaaaggc tacgactagc atagcccata cttgaaaggc acttgctaaa agccctacta
     2881 aaaattggac ccattctttc cgctgataag aaagtttcat ttggtacaat cctaagaaaa
     2941 gccacgtgaa gaaaatagct gctgtaccaa gtgtgtaagc aaagttataa gcactgtcag
     3001 tgaataataa tgaaagtaaa aagacattcg tacataacgt cgtcaatacg atagcataag
     3061 ttggtgcccc atttttgttg agttttcccc atgctttagg caaaagtccg tcttcagcca
     3121 tgtttcccat tgtttcaatc ggtaagatcg tccatgagag ccaaacacca actaaagaaa
     3181 tgatcaaacc gccgttgatc acgactgctc cccatttacc aaccatcatt tcaaacacat
     3241 agcccatagc aggctgcgcc ttgatcgttt gcagctgatt catcgtcatt gtcccatatg
     3301 gaagcatgga aaccaagacg taaataataa tcaagccgaa tatccctaga atcgttgctt
     3361 tcccaacatc tgattgtttt tttgctcgtg aacctacgac agaggctcct tcaataccaa
     3421 caaataccca aagcatcacc atgatgcaac tcaatacttg actggaaaac gggatcggct
     3481 gtccattcgc attcaatata atgtcgctag aaaagttatc aatgaattgt tggatcgaaa
     3541 acgtgctgat agctactaca ataaataaaa ataatggcac taatttacag aatgtgacga
     3601 tagcattgac gaaactagca ttttcgactc cccgattcac tagccatgca tatccccata
     3661 aaaagactgt actcatcacg atcgatgcca gattttgccc accttcaaat actgggaaga
     3721 aatatcctaa tgcgctcatc aacaatgttg caaaagctac gttgcctaac cagcatgtga
     3781 gccagtaacc ccaaccgctg ataaaaccag cgaaacggcc aaaaccttct tctgcgtaac
     3841 taaagatccc gccttttaaa tctggacgtt ttttattgat attattgatc gacaaggaga
     3901 gcatcaacat tgctgttcct acgagtaccc aactgaaaat agctgggccg ccagccgatc
     3961 cggagatgtc cgtcataata ccaaatacgc cacctccaat cgctgaacca attactaagg
     4021 cagcaagtgg aattaaacca attccttttt tctgttgctc tgtatccatg taagcatcct
     4081 ccaaaataaa accgctttta tcaaaaagta taacaaacct tttttatttt tacttaatta
     4141 ataaaaactt taatagaaat ataattctac cggctttttt ctcattatac aagcaaagcg
     4201 cctagtccct ttccaaatta caccaccaaa aaacttaaaa aagatgctat actaaaagga
     4261 aagaattgga ggatgatcca tgaacatttt atatatatcg atttcaggaa atacgcgtgc
     4321 ctttgccaaa catctagcag aatatgctga aaaaatgcat gcagaagatc ctgtcaatcc
     4381 tgaagttact ttaaaagaaa tccatgaaaa tagtgatttt acgaaagaaa cagaaccatt
     4441 ttttactttc gttcctactt atttagacgg cggaaatggt ttagataacg gtgatacaga
     4501 aatcttgacc gaaacgatgc gtgaatatct ggaacatgaa gacaatcata aactttgtct
     4561 tggtgtagtc ggtagcggaa ataaaaactt caataatcag tattgtctaa cagccaaaca
     4621 atatgcacaa acatttggct ttcctttctt ggcagattat gaattacggg gaacaccatc
     4681 tgatgtgaca cgaatttatc agattttagc agaaaacaat aaataataaa aaagaagaag
     4741 ctgaaaaata gatttcagtt tcttctttcg tttctattgt ttctatcata ttaagctttg
     4801 acatccatca caggtgcttt tgcttttgct ttacctgttt tttccaagac aaagtttttt
     4861 acacgatcca tattggttac caagacgatc atggatgttt ctttgcctgc tgctttgatc
     4921 gcttctaagt cagcttctgc tacaagcgta tcaggtgtca cttgctgccc ttctgttact
     4981 ttcacatcaa atggcgtacc gcctaagtca actgtgttga ttcccatatg aagcaagatt
     5041 tctaaaccat ttggcgttgt gatcccaata gcgtgtttcg ttgggaagac agagctgact
     5101 accccagtaa ctggagaata gatatgtccg ttttctggtt ctacaaagta gccttcaccc
     5161 atcatctttt gtgcaaaaac tggatcactt gctttatcta aagaaacaac tgttccgtca
     5221 gcaactgatg agaggatagt cacactttca gctgaagttt gaggtactac tttttcaggc
     5281 acttcattta ctgtggctgt ttcagtcgct aattcttctt tttctaaacg gtctttagga
     5341 ataccgaaga agaatgtagc aacgaagcca ccagcataag ctgccaataa tccaaggatg
     5401 tagcctaacc atttaccgtc agagatcaat gggatcaaag cggctcctga aggtccaata
     5461 gcgatagccc cgacatttcc gatcattccg atcactgccc cgccgatacc tccaccgata
     5521 caagcagtaa taaatgggcg tcctagaggt aatgttactc cgtaaatcaa tggttcgccg
     5581 attcctagga tcccgactgg taaagctcct ttgatcattt ctgttaattc tttatctttt
     5641 ttgcagcgaa tccataatgc caatgctgca cccacttgtc ctgcaccagc catcgcaaga
     5701 attggaagca atagcgtacg accagtttca tcaatcattt gcacatggat tggagtcaaa
     5761 atttgatgca gtccgaacat aaccatcggc aagaataatg tgcctagaac gaagccggca
     5821 aatgctccac caacgccaag tacccagttg atcccaccga ccatgccatc agaaatgaaa
     5881 cctgctaaag gcatgatgaa gaaaatttca atcagaccga tggcaatcaa tgaaagagtt
     5941 ggtgtaacga tgatatctac cgcatcaggt actactttat gcagtttttt ctcaacgatt
     6001 gacaatagcc aaacagcgaa gatgacacct aggatcccac cttgtccagc tgcaagagct
     6061 tcaccagtaa ataagttttt cagtggtgct tccggattca tccctgtcag catcacaacg
     6121 gccccgataa ctccaccaag agttggtgtt gcaccaaaaa cttgtgcagc attgatacct
     6181 gtatagataa ctaggtagct aaacatcccg tttttcaaga tgttcatcac atcgatatat
     6241 tgttgccatg ttgctgcatc aagtgttccg gctgtgacta agtttgctaa aactgcggca
     6301 atccctgcca cgatcccagt accgacaaat gcaggaatca ttggcacaaa gatattagaa
     6361 atgtctttta ataccgattt gattttagat ggtttttgtt tcgatttttg tgccgctttc
     6421 atttcttgtg cccgttcgtt tacacggtct tttccagatg ctgatgaatt tgctgtagct
     6481 gtacttacag aagaaagttt ttctcctaat ttcacgcctg ccaaatcaac catttgctgt
     6541 gctactttgt tcactttccc aggtccgatg atgacttgaa gctgttcatc ttctactaca
     6601 ccaagcactc ctggaatcgc ttttaattca gagacattta caagatcatc attgatgatc
     6661 ttcatacgca cacgagtcat gcagtggaca atgctttcta cattgcccat gccgcctgcc
     6721 cctttataaa tttgttttgc cagttcttcc aaagtcagtt cgttttttgc catggtcttt
     6781 tccccctcat aaggtccctt ttacaaatcc gtttgcttca gctaattttg cttgagcttc
     6841 ctctttatca ctatttgtca aaatcatgac gatagccagc ttaacatttt gatctgcttc
     6901 ttcaaagtac ttttcagctg tttcgtaatc agcttctgtc gcttgcatga tgattcgttt
     6961 tgagcgttcg attagttttt catttgttgg tttgacatcg accatcaagt tattgaatac
     7021 tttacctata ccaatcatac tgatagtaga aatcatattc aagataagtt tttgtgctgt
     7081 tcctgacttc aatcgagtag agcctgtcaa aaattctgga cctgcatcga cttcgattgg
     7141 gaaatccgcg tgtttactga tttcggcttg tttattgcaa gaaatcgttc ctgtgacagc
     7201 accgatcgat tttgcgtaat ctaatccacc gatcacatat ggtgtacgac cgctagctgc
     7261 gataccaata actatatcat tttttgtcaa attcagcttg attagatctt cttgccctag
     7321 ctcataagaa tcttcggccc cttcgacagc tacagtcatt gcttcttctc caccagcaat
     7381 tagacctatg accatttccg gttctacacc gaatgtcggt acgcattcag cagcatctaa
     7441 cacacctagt cttccacttg ttccggctcc catataaatc agacgtccac cttttttaaa
     7501 ggactcaatc gtgttagcaa tcactggttc gatacttgtc agttcttttt caaccgcagc
     7561 ggccactttc tgatcttctt tgttcattag ttgaacggct tgagaaatac tcatctcatc
     7621 taatccaaaa gtttcttggt ttctgcgttc cgttgttagg ttctctaaat tgattttgct
     7681 catttttatc ttccttcttt tcttataaat tgatagttca taccagcttt gatttggttg
     7741 atcaatggta gctcttcttt gataacttta gctactacat tcactttttc atccgctggc
     7801 aaatcatatt tggttaattg gatctcaccc atatagcgca aatacttttc gttatctaaa
     7861 gtaatagacc ctttcatacg cgtagctgta tttcttgctg ggataggcgg aatctctcga
     7921 aaacgtgcat ctacgcttcg tatgacatct cttgcatcgt cttgccgatt cgtatgcttc
     7981 cctaatatat attcgtaaaa ttcttcatct acaggttccg tatggagcaa gactgtctgt
     8041 tctttttgga aagaagcaaa ttgctcctga acgttttctg acagacctgc atctccgatg
     8101 taaacaatat ccgtgttact tgctaataga tctagagcgc ctgcaagagg atggatccct
     8161 cgatgttctt ccaaagttgg caatccttga tatagtgggc ctctcaactc tgtatctcct
     8221 ggtacaaaac cttggacaag gagaccttga attttgagcc attgattttt tttctgatac
     8281 cattctttgt ccagccctgt ttcgggacgt ggataataat tatgccaagc ttcaatctgt
     8341 gagaaatcag cttctgcttc tttcaattca tcgatatctt taggcgtgat cgtgctagca
     8401 ttcaaactga ttttcaattg atgtgaccag tcggcaatct gctgattgga aatatgataa
     8461 tccattctta aacctgttac accaattgct ttcaaaggcg ttagatcttc taatgaaaaa
     8521 cccgctcgtt ccaatgcctc tcctgaaata tcgaccatca attctagttt attctttttc
     8581 gcttcgcttc ctagttcgat caatcgcttt tgatatgccg aaacatcatc ttctggtata
     8641 tgcatggaag tgaaaatacc gacaaagcca ttggatgcca tttggcggat atactgtttc
     8701 ttctcttctg acaaagaatc gttcataaat attgaaaagc caaacatatt atccctcttt
     8761 ttctgtatcc gtcttcattt ctatgactag agtatatatc taaagaaaca aaatttcaat
     8821 acttttatta aaataattaa atttcatttc taaaggttga gtttatatta tcaaaagaat
     8881 tgtcataacc taaaaaaggc actgctaagc aaaagcagtg ccttgaaata ttttatttaa
     8941 ataacacaaa aacataatta ctcataagaa accaattctg tattatctac catgaatgta
     9001 aagaacccct cattaggcat cggatgaaaa taagcattga tcactcttat caaggcatta
     9061 tggcaaacta acagatagac attttcttcg tcttcgatgc attctttcaa taaaggaacg
     9121 atccgtgcat acacatctaa gactgactcc ccatttggga atcgtacagc gaacgctaaa
     9181 cgtgcttttt gaaagttctc atctttgctt ggcagtccat cataatcccc aaaatccatt
     9241 tctaccaatc gttcatcaac ggtcagcggc aagctgagac gatcagcaac tgcttgtgct
     9301 gtatctcttg ctcgttgaag aggggaatgg atgattttcg tgattggaac aggcaaatct
     9361 actacctttt ctgctagttt ctgtgcctgc atctgtcctt tttcagtcag cggcacatct
     9421 gcccgtccgc aaactaatcc ttgtgcgttc caagttgttt ctccgtgtct cgttacataa
     9481 agcatgtgat tctctccttc gttggcaaag ttccccttaa ccataccttg ttgttcttga
     9541 gattacaagg gttagattct ctcaagagaa ataatctcag tagatacttt atccaggaag
     9601 gtttgatcat gttccactag taacatggca ggtttcacgt tcaagatcag ttgttctaat
     9661 tgttcttgat tgaagacatc caaataattc aatggttcat cccatgtata tagttcagct
     9721 ggctgtgata aagatttagc caattccact tttttccgtt ggcccatact catctgctcg
     9781 atcttgttat gaaaaacatc tctttccatc ccaagctttc ggaggttgtt caaaaatgct
     9841 tggtagtcta cttggttttt ctctgcaaat tccgccaacg ttcctcgatt gtcttcataa
     9901 ttttggcttg cataactaat gctcagatgt tttggctggt atttttctcc tataaccttg
     9961 ccgttgaatg cccccagaag ataatggata atggatgact ttcctgcacc gttaggacct
    10021 gaaatcgcca cccgctgatg aggctcgatt gtaaaatgaa ttggctcgaa taacagattt
    10081 tcatacccta attgaagatc ttctacgctt aaaagtcgct tatggtgaga cgcttggcta
    10141 ttcatcgtca aagaatcgat atattcgata tcttttaata gtttttcttt ctcgctgatc
    10201 tgggtctcca tccgattcac gattgctttg gatcgtttca tcgtccgtgc agcatcagca
    10261 cccactgctc ctttattcac tcgtctagat tcagtatcga tgaatccgac ttgtttcttt
    10321 gttttatctc cttctcggga acgagaccat tcggcttttt cagctgctgt tttctttagc
    10381 ctactgactt cttttttcaa tttttcattt tgagccattt caaattcatc acgaagtttt
    10441 ttctgttctt catagataga gaaattccct tgataaagtt ccagttgact tttttcgatt
    10501 gctaaaacat ggtccactac ttcatcgata aatccccggt catggctgac cacgatgaag
    10561 ccttgttttt tctttttcaa ataagccgct acttgttttc taccagagat atccaaatga
    10621 ttcgttggtt catcgattaa cgggaaatga gtgtcatcca caaataaaag tgccagtagg
    10681 acttttgtct tctctccccc cgatagtgtg ctgaattctc tccataagat ttcaggatct
    10741 gtttgcatca attggagctc tctttcgatt tcccatatct caaaatccgt aatatcattt
    10801 aacacgtaat aggttaaacg ttctttatct tttgtctgtt gcgggaaata ggcaaattct
    10861 tgctgatgga ttacttgccc ttgataaggt agtttgtttt gtagaatatt cagtaaagtt
    10921 gtctttcctc gaccgtttcg tccgataagt cctagtttcc attgtgtgtc aaaatttaga
    10981 tttgcttgtt caaataataa tgtgccttgg ctgtcgtagc cgaatgtcag attttttatt
    11041 tcgattttag acattttcca tcacctcgta aaaaaagacc agtcctattt tcagaactgg
    11101 ctaaagagga tgcaagggtt ggatacacac aaaaagaccc atacatcgaa aaaaccaagc
    11161 caatcccgaa aatctgcaca ctgcttcctt gtctccacaa ggtacagtct gaatgcagct
    11221 ttcatcaatc gacttaattt ttcaatgcac ggatccctca ctttgtccta tatttgaaat
    11281 tacgatagca taaagaaaat gattttgtca actacttcgg gaggatggtg aaaaatctgc
    11341 taagtgtctg tcctaagatt tttgtttttt ctaatgtttg cctgtgttcc attgcttctt
    11401 ctaaacctat tggacccaaa tgaatactta agacaattgg gagaaaatca gaaagtcctt
    11461 ctagctcttg gtctctactt ccgcaaatag cgattattgg ttttcgatat ttttttgcaa
    11521 gcactgctac accgtatgga acttttccgt ggatcgtttg ctgatcgatc cgcccttctc
    11581 ctgtaatgat gacatctgct tgttcgatct ttttttccaa tccagtgatt tggctgacta
    11641 aatcgaaacc agaaaccatc ttcgctccca acaaaaaaag ggctccacca ataccgcctg
    11701 ctgctccact tcctggaata tcatctaaat ccacctccca gaaatctttt atttgctgag
    11761 caatctttcc tgcttgttta tctaagaaag ctagcgtcgt atctattcct cctttttgtc
    11821 ttccaaattg ccaagcagct ccttgttgac ctgtatatgg attggtaaca tctgcagcga
    11881 taatcaattt tactttagaa acaagaacgt ttcttcgatc aaaatgaagt tttttagtag
    11941 ataataaagg atttccttct gtgtaccctt ctaaagaagc acccaaggat tggagtaatc
    12001 ctagaccacc atcgctacac ccgcttcctc ctaaagtgac aataatttgt tctacttctt
    12061 gaagcagtgc atctttaata agttctccta aggcataact ggaagcaagt cggatagttt
    12121 gatccgttgg tttatcaagt agatctaacc ccaatgcttg tgcagattcg atgactgcgg
    12181 tgttttttcc ttgccaagtt gtcaataaat agctgatttt tcgctgacgg aataagagat
    12241 ccatcccatc aatggtacgc catgttcccc tgagcgcgta atagattgct tctgcagtat
    12301 tttctccgcc atctgcaatc ggcacattga tttttcttcc tttacgatct tcccagccat
    12361 ttagaactgc ctgatttagt tcaaatgaag aagcacttcc tttaaatgaa tcaattgccg
    12421 ccaaaatatc catctgtatc cctcactttc ttcttaagta agacactttt tttcattttt
    12481 ttcaaacaaa acaatgctat tttcttccat ttttctatat actaaaaggg aactcattga
    12541 ataaaggaga gaaaaaacat gaaaatcgca atagctgggg ccggagcaat gggcagccga
    12601 atcggattga tgctacatca aagcggaaat gaggtcctct tgatcgatcg ttggcctgct
    12661 cacattgaag ccattcgtac aaatgggttg attgctgact ttaacggtaa agaagtggtc
    12721 gcaaaactgc caatctattc accagaagaa atcatcgaaa gcaacgaaca cgtggacttg
    12781 atcgtagcat taacaaaagc caatcaatta gatgatatgt tttgttcgat ccaatccatt
    12841 attactgaca atacttatgt tttgtgttta ctaaatggat taggtcatga agatgtactg
    12901 gaaaaatatg tgccaaagaa aaatatctta tttgggatca cgatgtggac agctggtcta
    12961 gctggtcccg gaaaagtcac attgttaggt gatggagaaa tcgaactaga gaacttggaa
    13021 ccagaaggcg aagcatttac aaaaaaagtt gtagaagtct ttcaagaagc aaatttaaat
    13081 ccgatctata gccataacgt tcgttattcc atttggcgaa aagcttgtgt aaatggaaca
    13141 ttgaatggtt tatgcacgat tttggattgt aatattgctg aactaggcgc gcaaaaagcg
    13201 gcagagagca tggttcgaac aatcgtatct gaatttgctt ctatcgcagc aaaagaagga
    13261 atcatactag atcaagaaga agtgtaccaa catattgctt ctacttatga tcctgacaac
    13321 atcgggttgc attacccatc tatgtaccaa gacctgatca aaaaccatcg tttgactgaa
    13381 atcgactaca tcaatggtgc catttggcga aaaggacaaa aatatgatat tgctacacca
    13441 tattgtgcct ttttgaccca attagtccat gcaaaagaag gaatccttgg cgcagaataa
    13501 aaggctctct gatgagaatc agctgaatca gcagctggtt ctcttttttt aagcccaata
    13561 aaagatcaaa aagaaaacac aactctcatg atgccataag aattgtgctt gtttgtttca
    13621 gtacataatc tcttcaaaat gtaggttttt tatttgtttt caacagagga gaggtcatat
    13681 tcgtattgct cataaatagt ggaatcgtta gtgataaatc gttttgccca attttctgca
    13741 tcataaaatt ccaatagctc tgataggatt ggcttaaatg gtgtatgcag aaattccggt
    13801 aattcatcta aatcacgttc tcgaagcaaa aaaatatttt caggacgata aaaatgataa
    13861 tgagcaatcg cttgattatc cgtcactagt ttcttttgaa aatacatgga ttcaaatgtc
    13921 cgcaaagtca ctccttgctg accctcccgc aacagttcca gaatcccgcg cgattcccct
    13981 gtttttttca ggtactcttc ataagtaaga tatccaggtt gttcattccc tcgatgtgga
    14041 agaatgaaat agttggtcgt aacatctctt tttataaatt cctgcttata gctttctgct
    14101 cgttcttttc tcccattatt cgttgcacca aaaaatagat ccgtctttgg ttcgctagaa
    14161 gaaaaattca tatgtttcga ataaaatgaa ctattatgtt tcagcccaag ttcttttgct
    14221 tccaaaaaat caaaatgata aatctcatcg attgccggtt catcttgtgc ctgcttcagc
    14281 atcgcctcgt gttctgcttt gaagtgattc caaaaaaata acacaatttt tgcattggta
    14341 aatgggcgca cgcgtcgagc catctccata tcaaatccat aatctaaaaa aacgatcagc
    14401 tgataatttg ccaactgttg tctagctgat ttaatgaatt ccgactgtag taaatctttt
    14461 ccggaccaaa catctaaaga gcggatggaa ggcaaaaaat aaagtgattt attttttgca
    14521 actagcagta cgtcattttt gttcatcttt tccgcctcac tttctctgtc actttttcct
    14581 atgatagcct gtttaaatag taaaaagcca gtaaaacgtt tcctatattt gtatgaaaat
    14641 ttaacatttg tatgaaaata tgatgaaata aatcattctt ctattaaaat tcgcctaaaa
    14701 gttttctgaa tagttgtaat tgtctgaaaa tttgttatca ttagtcattg ttaaatattg
    14761 ctatttattc ttaatataaa ttttgattgt gaatttaaaa gaaagaggtc atttatggaa
    14821 gataaaaaat tgaaaaaaca acttagctcc agacatatca ccatgttagc tttaggtgga
    14881 gcaatcggtg caggtctatt taaaggaagt ggcgaagcga ttgggatcgc cggcccttct
    14941 gtattgattg cttttttgat tggtgggctg attttatata tcgtgatgaa aggtctggga
    15001 aaaatcgttt taagcggtgg cgacacgcat cacggattat ctggtttgat acgcccttat
    15061 ctaggtgctc attctgctga ttttactgat tgggtctact ggtctatgtg gattatcaat
    15121 atcatcgctg aagctgttgc agctgcttcc tttttgcagt tgtggttccc gcacattcca
    15181 gcctgggtat ttgtttttat gttggctgtt ttaactacga tcatcaatct ttattcggta
    15241 cggttattcg cagaaacaga atattggctg gcttttgcta aaatcagtgt cattatctta
    15301 ctgatcattt ttgctactta tcttgtcggt cagcaaatgt tgggcacagg cgttttccct
    15361 acccttcagc aattgactga ccatggtggg tttactcctc atggtatgaa gggaatcatc
    15421 agttctttgc ttgtagttat ctattcttac ggcggttctg aactgattgc tatcacggta
    15481 agcgaagcag atgatcctaa acgtgcgatt cctaaagcaa tcaaaggtgt catgggaaga
    15541 atcatttcct tttacatcat tcctttgttt ttgcttttga ttctttttcc ttggaatact
    15601 ttagcaggca cgaatgtcag tccttttgtg atggtatttg aaaaaatgaa tatcccattt
    15661 gcagcggata tcgtcaattt tgtgattgtc cttgctttgt tctcatctat caattcgggt
    15721 gtgtatgcat cttcaagaat tttgttcttc cgtttgaagg atcgaaaagg ttcctctaga
    15781 aaactagctg ttttaaacaa gcatcaagta ccgcaacgtg ctgttttatt ttgtaccagt
    15841 gttctttatc ttggtgtagc tctttcttat tttgtcggtg acaaattatt tggctatctg
    15901 gcaggttccc tttcgtatac agtattattg atctggatca ttatcagtgc ggcatccttt
    15961 gtactggcac taagaaaagg aacattatgg gataaaggaa tcagcttttt tgcattagct
    16021 gtcttaggat tgattttcct tggtatcctg ttcacgaact ccattggggt aactgtattg
    16081 actggcctgc tttacctgtt catttatttc agttatcaaa aaaagaatga tgcgttcgta
    16141 ttgacgaatg aatgaaatcg atagaaaatt ttattttatt tatgtggaat agatggagct
    16201 attgaagaaa ggagttgtga caaaagtctt gttgcaactc ctttttccga ataaacagtc
    16261 tcatgacctc ttggtaaacg gtgttcaaaa accctctgtg caaaataatt gattgttctc
    16321 catgctcatg acatgataga agaaaggaat gaaaaaaagg agtttgtttt aaatgaaaaa
    16381 gtattggaag tttttgttga tgatcggtgt ttcaacggta gttatgtttg gtatgatgta
    16441 cttgaatgtc tatgcgctcg atcatatctt cttcagtcgt acacgagtat tcatggcgtt
    16501 gatgatgggt gcagtcatgg cagtcatcat gctgttgttc atgtggaaaa tgtatgaaaa
    16561 caaaaagata aatgttatga tcttagccgc tagtcttctt gtttttgccg gttccttgtt
    16621 tatggtacga agccaaacta ctgtcaacga cacagcttgg atgaaggcca tgattcctca
    16681 tcattctatc gctatcctgg caagtaaacg agctgattta aaagatcctg aagtaaaaaa
    16741 attagcggaa gaaatcattc aggcacaaga agaagagatc gacaaaatga aaaaaatgat
    16801 caaagaaatg gaatgaccat tatccgtcca aataaaaaaa gaggatgttc cagaagttat
    16861 cagcttcaag aaataagaag aagctacttc tggaacgccg tgaataaggg gttgtgaaaa
    16921 agctgccctg catcaagtaa taagaacagc caaacaaaaa tagcttctcc tatttttcgt
    16981 ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattaa gaggttgtaa
    17041 aatcagcgtt ctgacctgag tattagagag acaaatggaa aaatagttcc tcatattttg
    17101 ccattttttg agctaatacc gagggtcagc tgattgaaca ccgtttatcg agaggttgtg
    17161 aaaaagtcgc ttagtttcgc gtattaagaa aaattttgtg aaatggcttt ccacattttt
    17221 gtataaattt ggcttaatac cgagaaactg gcttttgaat accgtttatt cgtaaaaggg
    17281 attgtgacaa aagtcttgtc acaatccctt tttatgtttt tattgttcta attgcgctgt
    17341 cttgcttact gcttcttgcg gtttcacaaa tttttttgag attccccaac ctgtgaaggc
    17401 actgaccatc gaaaacacag gagaaagcaa actgaaaaat acgaatggca agtaagtgag
    17461 tgtaggaata ccaaatgtac ttgctacgaa acttccggca attccccaag gcactaagta
    17521 gttgattact gttccaccat cttctagcgt tctacctaat acagttggat caaatcctgc
    17581 tttttcataa gtttctttaa atgcattacc aggcagaata acagatagga attgttcacc
    17641 aataaaaata tttactccga tacttgtcat caatgttgta aaaatcaaat ttggtgtgtt
    17701 cgtcatcttt cttgatactc gttccatcac ggttgagatc aagccaaatt cgatcaacaa
    17761 tccacctaat gacaaagtta gaataatcaa tgacacagta ggcatcatac tttcgattcc
    17821 tccgcggcta agcagtgaat cgatttgttg gtttccagta tttgaaacaa accctgattc
    17881 gatgacccca gccaacttcg ttaaggaagt ttgtgggttt tgaataaaaa tcattcctac
    17941 tgctacagcg atattcaaca aaatcgttaa aattgccggc acctttttcc atgcacacag
    18001 taacattaaa aataaaggaa tcagtgacca gattgaaata gtaaagtttg cttctagaac
    18061 atcagtcact tcacgaatag atgaaagact tgcggcttta cttgtgtgtc ctaaaatcat
    18121 gaataaaaag aaagaaacaa caaatgccgg gacagttgac cacatcatat gcttgatgtg
    18181 tttgaacaaa tcggcatcaa caatagctgc tgctagattt gttgattcgg ataatggaga
    18241 catcttatct ccaaaaacgg cacctgaaac aatagctcca acaaccagcg ctggatttaa
    18301 gcctagtgtt gtcccaatac cgaaaaatgc aatcccaatc gttgacataa cagtaaaggc
    18361 actccctaca gcgctaccta cgattgcaca cacaacaaat acagaaggga caaaccactg
    18421 gacactgatc aatttgaacc caatgaccat tagagttgga atgatccctg cttgtatcca
    18481 tacagcaatc aatgaaccga ctaaaataaa aataaagatt gggatgattc ctggtttgat
    18541 gccattgatg attccttcgt gcagccaatc tgtcgggtat ctttttatca ctgcatacaa
    18601 catgaccatt ccgatcgctg ctaagatcgt gatattaggt gagaccttta atccgacaac
    18661 gccaatcgca atgcctaaaa caataaccat taatacaagt gttgcttcta aaaaacttgg
    18721 tgttcctttt tttcccatga taatctctcc ttttttagtt gttcttatgt tctaaaaaag
    18781 ttaagatatc ggattactcc gccgcaattc atccgcgatt cctcctttat aaaaataaaa
    18841 agacctgttt taaactaagg acgaatgact ccgcggtacc accttaatta aaaacagatc
    18901 tgtttttcac tttcaaactt ttattcaaat gtatggactt tccatgtaat tcgtcaatta
    18961 ggcttgatcg gttcacagcg accaccgact ttctgaacaa aacacctaac cgctactgcg
    19021 tgaaaagttg acttgtacga agtttacctt ccataagatc atttgtcaat gaaaaagaaa
    19081 atattttttc atttttctga aaattatttt tataactttc acacacaaga ttatttttta
    19141 ctagaaagta gcgccctctt gtttttttct tgctactatt tcttgatgtt cttgcttgat
    19201 tctttctaat agctttggct ctttcaacag atcaagagct gtattggcga gtagttctgc
    19261 tgcaatcgca atagaagcaa ggcctttgtc acttttagca gcagctttga attcttctgt
    19321 gtgtccggca atatatgtct cagagattgc taccgtaggc tggattgttg gtatgacttg
    19381 gctgacattt cctacatcag aagaaccgag acttgttctt tgttccgttt ctatttcttc
    19441 atctggaaca cctgcttctt ttgcatgctg gaagaataag tcatcaaaag aaggagtaac
    19501 gatgacatca tcaactgcat tttggaacaa tccaaactca aaagtcgtgc ccgtacttat
    19561 cgcagcacct cgtacgatat tttccacttt ttcatatact ttatctaatg tttgccgatt
    19621 tcctgcacgt aaatagaaac gtccagcagc gtaatctgga atgacgttgg cagctactcc
    19681 tccgtctgta atgatcccat ggatattgac atcttttggc aattgcaagc gtagagcgtt
    19741 gattccatta aacacttgga tcaatgcttc caaagcattt actccttttt caggtgctgc
    19801 tgctgcatgg gaagcgacac cgtaaaattt gatgtcgact ggatcattcg ctaaagattc
    19861 tgtcgttttc ccataacgaa aagctggatg gacgcataac gctacatcga catcctcaaa
    19921 aaaaccttct cgaacaaagc tgcctttagc tgatccgttc tcgccccctt cttcccctgg
    19981 ggtgccatat acacggattt ctccaccagt ttcatcaatg aattgcttca ctatgctggc
    20041 ggctaatacg gagtaagtac caaacaaatt atgcccgcat gcatgaccaa tacctggtaa
    20101 agcatcgaat tctgccaaaa aggcaattgt gggaccgggt ttattggaaa tataacgtgc
    20161 atcaaaggct gttcggtgtc cagctacttc tttttctacc caaaaccttt ctttttctaa
    20221 ttgtttaatc aacgctgctg aggaataaaa ctcatagtta cttacttcag gatgttcgtg
    20281 gatatctaga gcaagtgctt gatattctgg taaacgatat tggattagat ccttcaattg
    20341 gtttgttgag attgttgttg tcataaaaaa ttcctcctat gctagtcatt tttcttattt
    20401 tctcccgaat gtttcccatg caggcacgct tgatcctttt gatgttttct cgattacttt
    20461 gactgtgtct tctgtttgat aagcgtctac tattttttga tacgttttat tgttttcatc
    20521 ttcttttctt gcaacgatga tattcacata aggacgtgca tggtcatcca ctggttctaa
    20581 gaagatggca tcagaagcag gattaaatcc agcatctaca gctacaccgc tattgatcac
    20641 tgaaatgtct acatctgaca aagcacgtgc tgtttgggaa gcatctaatt cagaaatgtc
    20701 tagatttttt ctattctcgg taatatcaga aacagtgggc gcttgtcctt ttgccttatc
    20761 cactttgatc aatccagcag tttgcaataa cagaagagct ctgccgccat ttgtaacatc
    20821 attagggatt gctacttttg ctccgtcttt gatatcagct acatccttca aagtgttaga
    20881 ataaattccc agcggtgcat tgacagtatt tccgatcgat actaaatcag ttccgtgttc
    20941 tttattgtaa ttgtctaaaa agaattgatg ttggaaagaa ttcaaatcaa tcgatccatc
    21001 tgctaaagcc acatttggtt gattgtaatc tgtaaatttc acgtattcca aattaatgtt
    21061 ctcctttttc aaacggtctt tcacgttgtc ccatacatct gtatcttctc caataatccc
    21121 tagttttacg atagtttctt ttttgtcagt tgtatctgct tttccttggc tacaccctgc
    21181 caaccaaata attgctacaa atgtaatgac tacaaaacga ataatttttt tcattttttc
    21241 tcctccactt tcaatgtgta atttttttaa tcagttgatt actgataaat tgacttaaaa
    21301 aaactaagat tagaatcaat aatgtagcca caatcgttac atctgtttgg aatcgattat
    21361 accctctcga tatagcaaga ttccctaatc cgcctgcacc tactgctcca gccatggtag
    21421 tcagtccgat cacgttgatg atagtcaacg ccgagacccg aatgatacca ggcaaacctt
    21481 ctaacaaata cacacgtaaa atgataccga ttggactggt tcccatcgct tctgccgctt
    21541 cgatcacccc aggatcaact tctagcagtg cgttctcgat ttgacgagca aaaaacggaa
    21601 caactccaaa aatcaatggc actaaagctg ctttcgcacc aatcgtcgtt ccaactaaga
    21661 aacgagtagt caatgctaaa agagaaagca agataatgaa agggatggaa cgaatgatat
    21721 tgatcagctt atcaagtatt tcaaatagca gacggttagc tagtaatcca ttagggcgtg
    21781 tcactactaa cgtcacacct aagatgattc caatcatccc gccaatgaat gctgtccaaa
    21841 aagacatata tagtgtttgc ttcgtcgctt cgacaaattc atccggaatc aaataaacat
    21901 tgggcaaata tgttttaaag aaatcagtca ttttatcttc ctccttcgat cactttgata
    21961 cgactatcta aatcgttttt ttgtaaaata tttattttta caccagattt gatcaaataa
    22021 tccaatgctt ttttccgctg tttggtttct ccagctaaag taacgatcaa actccctaac
    22081 ggcgtttctt gtaataactc tacattgcca tataaaatat tcgctgttac ttgatatttg
    22141 ctgtacagat gagcgattag aggttcgttc gtctgatttc caatataact taattccacc
    22201 agccatttat tggcgatctg atcggcaaaa gcagaatggg caatgatcgt ttctaatgct
    22261 tggtcaagat gagtagctgt tcgaataaaa tctttggtca attcttcttc aggattgctg
    22321 aagatctgga tgctgctacc tttttcaacg attcttccat cttccataac cgctaccttg
    22381 ttgcagattt ctttgacaac ttgcatctca tgagtgatca ggacgattgt taaacctaac
    22441 tggcgattca atttttttaa caatgcaagg atctgaaggg tcgttttcgg atcaagtgca
    22501 ctggttgctt catcacataa taatacctta ggttcattcg ccaaagctcg tgcgattgct
    22561 actcgttgtt tttgtccccc cgacagttgt ttaggataag cagatgcttt ttcttccaaa
    22621 ccgacaagtt ccagtagttc attcactttt tgacgacgtt cctgtttcga ttttccagaa
    22681 tactttaaag aaaaatcaac gttgtcaaaa atagttcggc tttccattag attgaaatgc
    22741 tgaaagatca taccgatggc ctttctttct tttcgcaatt ctttgggaga gagctgactc
    22801 agattcgtct gattgacaat gacttctcct gctgttggtt tctgcagcaa attcatcaca
    22861 cgaactaacg tacttttccc tgcacctgag taaccgacga ttccataaac atcgcctttt
    22921 tcaatcgtta aatctacaga atctactgcc tgtatctctt tttctttttg tttgaaacat
    22981 acagaaacat ctcgcaattc aatcattaaa attcctcctt atctaaaaaa agcgtcctca
    23041 agaaaatgaa aatgtcattt tcttgaggac gcttttttgc gtgttaccac ctcatgttca
    23101 agctgtgttc acacacaact cctcttcaag tacgatcata ctctagcaag atatcggtcg
    23161 cagccgtcat cgccttacat ctatgctcgg gatgaattgc tcgatggcca ttttccaata
    23221 gtcgttcctt gcttcttctc agctaccgaa gctctctgtc aaaggttctt ctatcgtact
    23281 taccatttca atgcaaataa tattcaaaaa aaaagtcctt ttactaacat aaactatgtc
    23341 agtaaaagga cgacgtatcg cggtaccacc ttttttcgga agtttccttc cctcaagccc
    23401 aacatctcaa aaagagagat tggcgttcgt tttaacggtc gaacagttcc gaatttgcct
    23461 acttatcgac aaattattct ccaaggccat tttcagcttt tcttacttga ctcttctcac
    23521 cagccagagc ctctctgcaa agtttccaaa cttactttcc ttttcaacga aatcgatatt
    23581 ttgttgttgg gattaatcta catgaggaaa actgaaaagt caagtacgaa attcaaatta
    23641 ttttttctat atttattttt ctgttttttt atttaacatt tcaataacag ttcgaagatc
    23701 ttttccttcc gcaagaagtt tcaatttttc cttttcttcg acggtataat ttcggacaaa
    23761 actggaatca ggtatcaaaa caacgcctaa tcctgctcgt gaagcagctg ttgctcctac
    23821 taaagaatct tccactgcaa aggctgaact agcttctaat cctgttcgtt ccaaagctgt
    23881 cagataagga atcggtgaag gcttgttctc cgacacttcg tctccgaatg taaacgtgtc
    23941 caagtaatca gctaatttaa agtgatccaa gatatctgtt gctcttttcg taacagtggt
    24001 cgtagcaatg cctagtttca tatgacgttc ttttaagtct gccaatattt cttttgcata
    24061 tggttttaag tggatggctc cgcttgtcag ctgttgataa aaatattctt cacgcttcgc
    24121 tcggacttcc tgtacttttt cagggctacc tgctgctttc actaaaatat caaaactttg
    24181 tttccagttt tttccgcccc aagcgtcaac gatttctttt gggatatgga tatcaaattg
    24241 ctgaaatccc caaagccagc catcacgata cgttttttct gtgtcaacta acaaaccatc
    24301 taaatcaaat attgcacctt tcatctatac attaatcctt tccaaaacaa tacacatagt
    24361 ttagcacgac tggtccaaaa agaacagaag caaagaacca gccttctcgt ttataagagt
    24421 tcttggaatc gacggatata gattttcttc acagctgtca caagcgccag ataagcgatg
    24481 actgtagcaa ataaccataa ccagtaattg ccaggaagtg gcatcaagcc taattcagca
    24541 ccaaaacttg taaaaggaag gacagaaccg acaataattc ctaatgtagt gataacaaac
    24601 atggcaaatg ttgcgttgct ttggataaat ggtactttcg gtgtacgcag tgcatggagc
    24661 actaaggttt gtgtccatag tgattcgaca aaccatcctg catggaataa tgcgatgaaa
    24721 atcactcgct gtgaactatc caatgtatgg aaacttccgc ccactacagc aggacaaata
    24781 acaaaataca tcaacaagta agtagtaata tcaaatatcg aacttgttgg accaaagtaa
    24841 accatgaatt ccccaatact agatgcattc catttttttg gctcgtccaa atattcttta
    24901 tccatccgat cccatggcaa ggaaatgcag gaaacatcat agatcagatt caaaaacaaa
    24961 agttgtaaag gcagcattgg taaaaatggc aggaacgtac ttgcaatcag cacggaaaac
    25021 atattgccaa aattagaact tgctgtagcc ttgatatatt tcatgatatt cccaaaagtt
    25081 tctcgtccag aaagaaggcc tctttccaaa atcgtcaaat ctttttctaa aaggatcacg
    25141 tctgcagatt ctttcgcaat atccacagct gtatctacag aaataccgat atcagccact
    25201 ttcattgcag gagcgtcatt gataccatct cctaaaaatc cgacagtatg accattttta
    25261 cgtaaagctg tcgtcaaacg tgctttttgc tgaggattca attttacgaa tacttcatgg
    25321 tcttcagcta cttgtgctaa ttcatgatca ctcatttccg agattttctc accggtaacc
    25381 aattcattga tctctagacc tacttgacgg cacactgaac gtgtaactaa ttcgttgtcc
    25441 ccagtcagaa cttttacagc tacaccatgt tctttcagag ctttcaacgc ttgtttcgtt
    25501 gtttcttttg gtggatcaag gaaagcaaga taaccaatca aaaccatgtc tgactcatct
    25561 ttgactgaaa attctccaac gacgcttgga ttggtctttt gtgcgacacc aatcactcgt
    25621 aagccatctt cattaagtga ctgtactttt gcaagtacgg cttcttttcg ttcttttgtt
    25681 aatggtgtaa cagtaccgcc aagatcaata aatttagaaa tgttcagcat ttcttcaatc
    25741 gcaccttttg tgatcatttg tgttttacca gctggatcag caaccacaac actcatacga
    25801 cgacgttcaa aatcaaaagg aatttcatcc actttttgat aattgatctt atctgtatct
    25861 aaagtttctg ttgcttcatc aatgatagcc acatctaaca aatttttcag ccctgtttga
    25921 tagtagctat ttagatacgc atggcgcaaa acacgatcat cttcatttcc ttcacaatcc
    25981 atatgatatt caagaatgat tttatcttgt gtcaatgtcc ccgttttatc cgtacataac
    26041 acatcgattg caccaaagtt ctggatggca ttcaaatttt tgatcactgt tccttttttg
    26101 gccatcacac ttgctccttt gaccaaattc gtcgtaacga tcattggaag catttcaggt
    26161 gttagaccaa ctgctacaga tagtccaaaa agaaaagctt ctagccaatc cccttttgta
    26221 agcccattga tgacgataac tgctggagcc atcaatgaca taaatttgat cagtagcatg
    26281 gatgttttat ggatgcctaa ttcaaagctt gattgaattg gtttttcaga tagcgtttct
    26341 gctacatgac caaatagtgt ttcatttcca acagaaacaa tgattccttc cgcacttcca
    26401 ctgatgacat tacttcccat aaagacaaga ttttcgtaac ttgtttcgct ttgctgatcg
    26461 tcttcacata ctttcgcatg cttttctact ggatagcttt cccctgtcaa tgctgcttgg
    26521 gaaatgaata aatcctttgc ggacaataaa cgcatatccg ctggtatcat atcccctgct
    26581 gaaagccgaa caagatctcc acagacgatt tcttccatag gaatctctgt gtactttcca
    26641 ttacgtttta cagctgccgt tactttcaca agtgatttca gtttttcagc cgcttgattg
    26701 gaacgtacgg actgaatcaa cgtcattgtc ccgctgacga taaccatagt gaagataatg
    26761 gctacaccga aaagatcttt atctccagga gctgctaata catattccgt gatgaatgaa
    26821 ataattccta atgcgatcaa cactaatgta aatggcgtga tgtatgcttt caatatttct
    26881 acaatgaatg gtgttttttt gccatatgaa atcgcatttt caccgtaatt ttctcttgaa
    26941 cgttcgattt gttcttctgt caatccattt gctgatgcat ggaatagttc aaataattct
    27001 tgtttcgttt ttccagcaaa agctgcatag attttttgtg tatctttctt tttttccatc
    27061 tcaatctctc ctttgtgtcc aaaagacagc tgttctcgct gcttttggtc tttagctatt
    27121 catcggatcg atagagaaaa aactgaaagg ggctgagaac agatagataa tagccctggc
    27181 ctttcctcta gatccttgtt tttcgaatga ctgtcatcca tttgttctca tctcctttac
    27241 taagtaattt taattcatga cttccaatca tttttcgaat agttattttt ccaattagac
    27301 cacctccttt ttatttttca ccgatgggga tttgcggcaa caaaaaaacc tctcgccaaa
    27361 tactcagcga aaggtttgaa aaagcatatt aaaaaaagct tctcagatct catcgttgag
    27421 ttttgacact gtacaacgta aaaagcagat acctgctttt agctacctta tgaaaagcct
    27481 tatctcgaca attcctgttc tacccattgg cgtctctcga cgtttttggg cagtagccta
    27541 tgtttgtata ggagcctcac ctaacaaatc ttattcggtt ttccttcgtt aatataacaa
    27601 ctaaaaaaga ggatgtcaaa tcaattttcc atgtttttca tacttttttt aaaatacaca
    27661 cgttatattc ctgtctcacg cggttttatt tgtctcattt tccacctccc gctataatag
    27721 aaatatagta ggaaacgagg tgctgttatg gaaagaacac ttgttattat caagccagac
    27781 ggcgtgagac gccatttagt cggaagcatc atccaacgtt ttgaagcaaa gggactggcc
    27841 attgctgaaa tgaagtttga aactatgact cctgaattag caaaagaaca ttatcagcac
    27901 ttgactgagc gcagtttttt tgatgaactg atcgattaca tgacttccgg tcctgtcgtt
    27961 tatcttgtgc tggtcggcga ggaagtgatc gatatcgtcc gtaagatggt tggagcaaca
    28021 aaagctgccg atgctgttcc aggtacgatc cgaggagact acgcgctgcc ggggactgaa
    28081 aacatcattc acgcttctga ttcccgtgat gctgcggtaa aagagatcgc ccgctttttc
    28141 ccgccattcg atataaacaa accgtctgaa aaagcggcga agtgatacaa attctaaact
    28201 gctggtctag ttgctgggtt cagcaatggc cagcagtttt ttctctaagg acctccgttg
    28261 attctttcct aacatatatt cgagcactgt gaagaattct tggtagtctt tcgtcttttc
    28321 ttcccctaaa gcagcagaaa tcaatctttg ataataatct gccatcaatg tttcatagat
    28381 cactgtactt ctttctttat gtgctaagct ccgtttcata tcttcattca cactacgtgc
    28441 ctttttcaac cgaccatttt ctatataaaa cgaagtcagg ttcattaaaa aagtaaagag
    28501 atactgctca tgattgttca actgtgctaa tcgtcgcatt ctttttttga ttttcaaata
    28561 attgttctca atgaaccgat cactgaatat ccaaggaaaa tcagctagaa tagatagttc
    28621 aaattgacca tagtctggta tctgcatcaa gtattcttca atgattcggc attcataacg
    28681 taaaaattca aattctgtct cagatacttc tgttttttcg accatgtgaa gattcaaaac
    28741 caaataatac agatcttctg ttttcttaaa atgcctacgg ttattgctga tctctttata
    28801 aggatcaaag ctgtcttctt gtacccgatc tagcattcgt ctttttttct ttaactttat
    28861 tggttcatcc agctggtttc gcatccattc atattctgca agtgtcatgt tcattcgctc
    28921 taaaaatgat ctcaatgtta caaatgacat ttttttcact gaatttgtct caatcccgac
    28981 tagagaagtc ttaaaacaga ttccttccgc taattgggac tgtgtcaact tcttgttgac
    29041 tcgaagtttc ttgatcaatt caccatcata cataatagtt ctccttttta atttaaattt
    29101 ttccacattc aataaataga tacgttaaaa aggaaaaata cacgaaaaaa ataaaaaaat
    29161 gtttttttca taaaaaatac caaccttcaa gcattagaat gttgtctaac tactgaaggt
    29221 tggtttgatt gtttattgat tatttttttg ctgttcaacg tctgttcttg ttactggata
    29281 taagttggtt gttgttttat taccattttt tgaaaagaca cttgtcgaat gttcgccttt
    29341 ttgcttttcg attgcttcat tacgttccgc ttcattttta tagtcatatt ttttcggatc
    29401 aatcgtctca aatccgtttg gcacataaaa ccgcaagaga ttttcctgat tcagcttatc
    29461 tgaaagactt aacgcagtct tgactttttc ctgatctttg gagatttcct catcgattcc
    29521 tgctgctttc gtatcgatga tttctcctgt ctgattattg taagcttttc ctcctaggac
    29581 ggtgtatttg ggtgtcacaa agttcccatt tcgaaaagca actacttgat catgctgaga
    29641 agataataag tctgtaccaa aatgaagata atctttatca tcgattccca atagatgtag
    29701 taatgtcggc aaaacatcga tttctccacc gtattcatga ttgactgtcc ctttagtata
    29761 accaggcata tggatcatca atggcactcg ttgcatctgt gcgttgtcaa attcatccca
    29821 tgtatctgga tctttaccaa gagcgcttgc taaatcttta ttatctgtat tcgagatccc
    29881 aaaatgatcc ccgtaaatca caaagattga attttggtaa ataccagatt ttttcaaata
    29941 cgtaaaaaat tgttccaatg attgatccag ataatgtgcc gttcgaacat aattatctac
    30001 tgtgctattg ccagtattca aagatggaaa gtcgaaattc ttgtcatccg tataataagg
    30061 cgtgtggttg gttacggaaa gaaatttcgt gtaaaacggc tgttgtagat gctcaagata
    30121 ttgggcagat tctctgaata aatctttatc taatatccca taacctaacg tttcgtcgga
    30181 ttcatcaaaa taagatcgat caaagaaatt gtcataacct aagtttttat atacatgatc
    30241 tctattccaa aaactagcta cattcccatg gaaaacagcg cttgtatagc cttgcttttg
    30301 tccaaggatc gctggtgcgg cttgaaagac attgtctgac ccaagttctg tgaataatga
    30361 gccttgaggt aaaccaaatg tgccagtttc caacatattt tcagcatcac ttgtctttcc
    30421 ctgtcctact tcatgaaaga aattgtcaaa gctgatcgtt gcttgattct ggaaaatact
    30481 atttaaaaac ggtgtgactt cttgtccgtc taccttcata ttaatcaaaa actgctggaa
    30541 actttctaaa tggagaacga tcacattttt ccctttagcg atcccaaatg tttcgggatt
    30601 tggctctgca taatgtttct ttgtataatc taaaacttca tcaatcccat tactgcttgc
    30661 atgcgcgcga acactgctag tcattcctgt tttgattccg tcataaatag tatacgcatc
    30721 gattcctaga tatttgacaa tatatgaacg gtcaaatgtt cgctgcagca attgtggacg
    30781 gttggcttcg ctcagcgcta gatttcctga aaaaataagc cctgaaagac aggtaacagc
    30841 gatagccatc ggtttgccga cagggttgct tttgataggt attttcttga ccttcaacca
    30901 aaccagtagc ccaatgaaca cagcgatatc cagccagtag atgacatcat ggggcttcat
    30961 caaagaaaag gagcttcctg acaatccttg cgacactttc gaaaagccta atacagattt
    31021 gactgtaatg aaatctgtga attcccgata aaaaataata ttcaagtaca acaaaagcgt
    31081 atttaaaata tcaatgatca aaagaatagc taacgctggc tttggctttt taatatacaa
    31141 agaaaggcca aaaaacaata aggtagtcgc aattgggttg atccacaaga taaagtactg
    31201 gatcgtacct tccacaccta atgaaaaatc tgcatagtag gcaatgatcg tcttcagcca
    31261 gaaaaagaaa acagctgtca aaacgattgc taaacgattt tggcacaatt ttttaagttg
    31321 tgtcacgtat atcacttctc tctattcaca ttttttctta ttttgctcct gaaaaatgaa
    31381 aatttcctaa aaaagttgtt atcttaagat ttttttattc tttctaaaga ttattccgtc
    31441 cacttttcac agcataaaac cgctgcgatg taagcgatta aactaatgaa gagataaaag
    31501 tcttgtggtg accgcttcac tccacggtat tcaaaagcca gtttctcggt attcgtacaa
    31561 aaaaagaggc tgtgacaaaa ttcttgtcgc agctccttat ttcttgatgc aggacagctt
    31621 tttcacaact tcttccttaa acggtgttct cctgccaact cctcggccac aagtcacaat
    31681 atttataaaa catgagaagc tgttttttga aattgttcct tgtggctcag agttaaacgg
    31741 caggctcaag acctcttgat acacggtgtt caatcagctg accctcggta ttagattatg
    31801 tcaggacgct aattttacaa cctcttggta aacggtgttc aaaagcttgc atctgcggta
    31861 ataagcccaa ccaaacgaaa aatataagga gctatttttt gaaattgctc cttgtggctc
    31921 agagttaaac ggcaggctca agacctcttg atacacggtg ttcaatcagc tgaccttcgg
    31981 tattagctca aaaaacggca aaatatgagg aactattttg ccgtttgttt ctctaatact
    32041 caggtcaaaa cgctgatttc acaacctctg tttttcattg tccataggat tgatattgtc
    32101 cttgtgatga ctcattcgta ataattgagg cttgattttc ttcacttccc gttatcgttt
    32161 gtccactgcc agaatctgaa ggacttgttc ctgtcgactc gtttttttga gaagagacag
    32221 ttgttttctt gacttgttca tttgtctttt tccattccgc taaacgatcg gcgaccactt
    32281 ttttgtctac tttttcagaa taagcggtga tcacttttcc atttttcaaa cgggctactt
    32341 tgtcgccatc atgagtcaaa agcaaaacgg tcaaatgctt gtctttaaat tgatattgtc
    32401 tgagagaaat cgcactaagt acgtcatcga tattaataaa ttgattttct tcttttcctt
    32461 ttggcaaata aatgtccaat tcgccttttt cgtaattggc agttatttta tactccgccc
    32521 accaataagt tgagaattct tttttgaaat tatctactga ttctttgctt gctacgattt
    32581 tttctgtttc tttctgagca agatctgctg tcatttttcc tgaaaatgct gcggataaaa
    32641 taagcagtaa gaaaaaaccg aataaccaaa agagaagaaa cggtcttttt ttcctcatgc
    32701 tttcctccac tcctatttaa ttttgactat tctattatat acaaaaaatg aaatagaaag
    32761 acaaaaaagt tgaaaaagat cgtaaataat tgaacttctt tttcacaaaa tggtaagaat
    32821 ggagaatttc acgcatttgc tctcaactat tctttcttaa gcctcaaatc cgtttttatc
    32881 tgatacgtct ttgaaccaat gacccgattt tttgattgtt ctttttccat cttgtgctaa
    32941 gtctactgaa ataaagccat atcgattctt atatgcattg gtccacgacc agttatccat
    33001 acatgtccac atatgatagc ctaaacaatt ggcaccttct tgaatggctt gatggacata
    33061 tttcagatga gattggacaa attcgatccg ataatcatcg tggatcatgc catcttcttt
    33121 gataaatcgt gcttcgtttt ctacacccat accgttttca gaaataaagc agtcgatatt
    33181 tccataatga tctcgtacat tgatcaaaat atcatagatt ccttgttcat agatctccca
    33241 tccgcgataa ggattcattt tacgaccagg catttcataa ttgtcgaaat aatcctcagg
    33301 cattggaccg tttttagtat cgattggtgt ttcttttgct tttacccggc gtggctggta
    33361 ataattgata cctaaaagat ctactgtatt ttctttgata accgtcaaat cctctgcatg
    33421 gatcacaggc atgagatcaa gttcttttac tatctcaatc aattcacttg gaaactcgcc
    33481 ttttacagct ggatcaagga atgaacgatt gaagaacgca tctgctattt gcgctgcttt
    33541 aacatcttca gggtttgatt cgtctcttgg atagcttggt gtcagattca agatgatacc
    33601 aataacacca ccttgctcca tttcatggta agatttgatg gctaacgctg aagccaacgc
    33661 ttcatgatag ccaacttgga tcgctttttt catatctgac tcttctggat aatgccatcg
    33721 atataaataa cctgcttcga ctggaacgat tggttcatta tgagtaaacc atttttttac
    33781 tttcgaacca aacaattcaa aacaactttt ggcatatttt tcataggctt caaccgtttc
    33841 tcggttcaac catccgcctt tttcttgcag atacatcggc atatcgaaat gatacagatt
    33901 cacaaagggt tcgataccgt tttcaatcaa ctcatcaaaa taacgatgat agaaacgaac
    33961 ggcttcttcg ttgattgttc cagattcatc aggtatcaaa cggctccatt ggatagatgt
    34021 tcgaaatgaa ttatgcccag tctgtttcat caactgtaca tcctcttgat acttcgtata
    34081 aacttgcgag gttttttcag gtcctacttg atcaaagaac ttttgcggtt cttttttgta
    34141 ccaataatca aaaatgttct ctccttttcc atcgcctgaa atccttcctt ctgtttgtgg
    34201 gccacttgct gcagaacccc accaaaaatt tgctggaaat tgatattttt tcatcaaagc
    34261 acctcttttt cttttttagt tcgttttagt tgattccatg tatgatattt tccatcacaa
    34321 tcactttttc attttcttcc tcttcttttt tgattcgttt taacagctgt tctgctgctc
    34381 gttccccgat tttatctgtt ggtggcgtga tcgtgatttt cggaaggata ctcagatcgt
    34441 ttttatctaa ataatcatag tgactgaacg caatatcctt cccgattctg acaccttctt
    34501 gattgaaaca ttcgatggca ccaatcgaca tattgtaatt gctgacaaaa atagcagttg
    34561 cccctttttc taataatgct tttgccccgt aatagccgct ttttctagaa tagtctcctg
    34621 taaagacctc taattggatc gatggatcat tttggaccgc ttctttggct cctgctaaac
    34681 gttctcttgc tacgtagtct gtttgagtgg cagcaataaa tccgattttt ttatgtcctt
    34741 gttcaccatg atgagcgatc acttttgcta cactttctcg atcattcact aatattgtat
    34801 caatacctgc tggtccttct ggacaattca atgcgataac tggaatactc atttttgtga
    34861 gcatttcaaa tccagaccac tcttcttcgg ataagaagac aatcagccca tcgatcacat
    34921 gcttcatcag gtattcaatt ttttgttcaa ttttttctgg ctgatcggca aacccgctta
    34981 ataataagct atacccattt tcttcagcta ctctttcgat ccctgagaca acttccgacc
    35041 caaaacggct ggaaatcccg tttatcaaaa gtccaataga gaagcttcga ttattcttca
    35101 accctttagc aatgatgttt tctttatagt ccaacgctga aatcgcttct gctatttttt
    35161 tcatgttctt ttccttcagt tgctgaccat tcaagtaacg ggaaactgtt cccactgaaa
    35221 caccagccat cttagccaca tcacggatcg atgccataca ttctcactcc tgccagttac
    35281 atttgatttc tctttcttgt tgttcgatta tgatgaccgc attctttgca tagtgttgct
    35341 tgtcatcaat atccatataa ctaaagtctt cagtagagca acttcctctc actattaatt
    35401 atacttaaac aagtatttga accggttcat ttttaatata gcattttttt aaaagattgc
    35461 aacacttttt taaaacgctt tctgaaagtg gcggattttt tgccttttta aaattttatt
    35521 ctaaaattta ctctatttgt gggtgttttg tgcgtttttc tcttccatcc gtttgaagtt
    35581 ttacattcca atggtataat ttctttatcc tatcaaatgg gggaagtatt ttgaagaaat
    35641 taatcgtact atttattttg tctgcgggac ttttgacatt gactggctgt gatttgtttg
    35701 gcggctctga ccaatcctcg gacgaaacaa ccgattcttc tgtcttaaaa gcggaaaaag
    35761 cagataccac caacagtgta tatacgctaa aagtacgtaa agatatggat ttatccgcgg
    35821 gacagttgac atttacagat aaaaaaatgg aatggacacg cacgtatcag acacaaggaa
    35881 ctgattcttc tgaatcatct gaagaaaaaa cagtgctgac agatatcaag atcactacca
    35941 aagatgacga atatattatt tcaggaaaag aaaataaaaa agaaacaaca gtcatattta
    36001 caaaaatcgg taattaccgt ttgaaggatg aggatggcaa catctacagt ctatagtgcc
    36061 gtctgttgga tcttcttttg tttctcccct tcttttctgc tatagtaaag aagcaagatg
    36121 attgtaaagg agacgaaatg gatgatcgat agtcctgatt attgtacatg taataacaac
    36181 aaaaatatcc atacagaatt taatgaccaa ggaagcttga gcatatgcga caactgtcac
    36241 aaactagtca aagattcatt taaaacattt gaatcttatc ctgttcaaaa ttatgtaaaa
    36301 agtagaagaa gataaacaaa tccaagcaaa actcactatg gctgattttt atgaacttca
    36361 aactcagctt cttcctttga tcaagcttca tcagtaaaac aaacagtgga tcatttcagt
    36421 ttacaaatca aagagtaaag atttatttat ttcaaatatt tgaatataaa ataaccaaac
    36481 gatagaaagg cttattctac atcacgtagt a
//
LOCUS       DAZKNH010000017        35959 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000017 DAZKNH010000000
VERSION     DAZKNH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.017"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..328)
                     /locus_tag="ACG8MG_000815"
     CDS             complement(26..328)
                     /locus_tag="ACG8MG_000815"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0374882.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="ACG8MG_000816"
     CDS             complement(353..2446)
                     /locus_tag="ACG8MG_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0374883.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="ACG8MG_000817"
     CDS             complement(2935..3699)
                     /locus_tag="ACG8MG_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFQ0374884.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="ACG8MG_000818"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="ACG8MG_000818"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFQ0374885.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="ACG8MG_000819"
     CDS             complement(4704..5780)
                     /locus_tag="ACG8MG_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0374886.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="ACG8MG_000820"
     CDS             complement(5804..6397)
                     /locus_tag="ACG8MG_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFQ0374887.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="ACG8MG_000821"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="ACG8MG_000821"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFQ0374888.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="ACG8MG_000822"
     CDS             complement(8067..8366)
                     /locus_tag="ACG8MG_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFQ0374889.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="ACG8MG_000823"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="ACG8MG_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFQ0374890.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="ACG8MG_000824"
     CDS             complement(10121..11032)
                     /locus_tag="ACG8MG_000824"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0374891.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="ACG8MG_000825"
     CDS             11152..12009
                     /locus_tag="ACG8MG_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0374892.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="ACG8MG_000826"
     CDS             complement(12196..12558)
                     /locus_tag="ACG8MG_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFQ0374893.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(12747..12929)
                     /locus_tag="ACG8MG_000827"
     CDS             complement(12747..12929)
                     /locus_tag="ACG8MG_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374894.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            12904..14343
                     /locus_tag="ACG8MG_000828"
     CDS             12904..14343
                     /locus_tag="ACG8MG_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0374895.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="ACG8MG_000829"
     CDS             complement(14350..16902)
                     /locus_tag="ACG8MG_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFQ0374896.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="ACG8MG_000830"
     CDS             complement(17016..18347)
                     /locus_tag="ACG8MG_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0374897.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="ACG8MG_000831"
     CDS             complement(18313..19791)
                     /locus_tag="ACG8MG_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0374898.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="ACG8MG_000832"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="ACG8MG_000832"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0374899.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22401..23828
                     /locus_tag="ACG8MG_000833"
     CDS             22401..23828
                     /locus_tag="ACG8MG_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0374900.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23920..24285)
                     /locus_tag="ACG8MG_000834"
     CDS             complement(23920..24285)
                     /locus_tag="ACG8MG_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0374901.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24282..24647)
                     /locus_tag="ACG8MG_000835"
     CDS             complement(24282..24647)
                     /locus_tag="ACG8MG_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0374902.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24839..25087)
                     /locus_tag="ACG8MG_000836"
     CDS             complement(24839..25087)
                     /locus_tag="ACG8MG_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374903.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25086..25214
                     /locus_tag="ACG8MG_000837"
     CDS             25086..25214
                     /locus_tag="ACG8MG_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFQ0374904.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25271..26965
                     /locus_tag="ACG8MG_000838"
     CDS             25271..26965
                     /locus_tag="ACG8MG_000838"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFQ0374905.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            27065..27238
                     /locus_tag="ACG8MG_000839"
     CDS             27065..27238
                     /locus_tag="ACG8MG_000839"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374906.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            27616..28749
                     /locus_tag="ACG8MG_000840"
     CDS             27616..28749
                     /locus_tag="ACG8MG_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFQ0374907.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28780..29100
                     /locus_tag="ACG8MG_000841"
     CDS             28780..29100
                     /locus_tag="ACG8MG_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFQ0374908.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29129..29593)
                     /locus_tag="ACG8MG_000842"
     CDS             complement(29129..29593)
                     /locus_tag="ACG8MG_000842"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0374909.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29725..30978)
                     /locus_tag="ACG8MG_000843"
     CDS             complement(29725..30978)
                     /locus_tag="ACG8MG_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0374910.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(31007..31324)
                     /locus_tag="ACG8MG_000844"
     CDS             complement(31007..31324)
                     /locus_tag="ACG8MG_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0374911.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(31350..31670)
                     /locus_tag="ACG8MG_000845"
     CDS             complement(31350..31670)
                     /locus_tag="ACG8MG_000845"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0374912.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(32134..33549)
                     /locus_tag="ACG8MG_000846"
     CDS             complement(32134..33549)
                     /locus_tag="ACG8MG_000846"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0374913.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(33566..34882)
                     /locus_tag="ACG8MG_000847"
     CDS             complement(33566..34882)
                     /locus_tag="ACG8MG_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0374914.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(35032..35730)
                     /locus_tag="ACG8MG_000848"
     CDS             complement(35032..35730)
                     /locus_tag="ACG8MG_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFQ0374915.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(35773..>35959)
                     /locus_tag="ACG8MG_000849"
     CDS             complement(35773..>35959)
                     /locus_tag="ACG8MG_000849"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374916.1"
                     /translation="SNEDRLLLNIIFFVVQNKNDRRLGVNFILVFFMVNIFFVCMSMN
                     GLINFVYKKSSDDSNVL"
BASE COUNT        10869 a         7511 c         6138 g        11441 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg
    22081 gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa
    22141 aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac
    22201 atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata gaattatctg
    22261 ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa
    22321 attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa
    22381 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
    22441 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
    22501 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
    22561 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
    22621 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
    22681 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
    22741 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
    22801 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
    22861 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
    22921 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
    22981 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
    23041 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
    23101 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
    23161 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
    23221 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
    23281 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
    23341 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
    23401 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
    23461 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
    23521 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
    23581 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
    23641 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
    23701 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
    23761 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
    23821 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
    23881 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
    23941 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
    24001 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
    24061 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
    24121 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
    24181 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
    24241 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
    24301 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
    24361 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
    24421 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
    24481 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
    24541 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
    24601 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
    24661 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
    24721 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
    24781 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
    24841 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
    24901 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
    24961 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
    25021 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
    25081 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
    25141 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
    25201 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
    25261 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
    25321 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
    25381 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
    25441 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
    25501 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
    25561 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
    25621 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
    25681 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
    25741 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
    25801 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
    25861 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
    25921 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
    25981 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
    26041 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
    26101 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
    26161 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
    26221 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
    26281 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
    26341 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
    26401 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt
    26461 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
    26521 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
    26581 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
    26641 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
    26701 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
    26761 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
    26821 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
    26881 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
    26941 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
    27001 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
    27061 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca
    27121 agtaataaga gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt
    27181 accgcagatg caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt
    27241 tcgagtatga agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt
    27301 aataccgaga aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt
    27361 gaaaacaact attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac
    27421 aactatttca atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa
    27481 tacataagac tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa
    27541 gatctccttt aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg
    27601 aaaggagcat ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga
    27661 aacgtagatg atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca
    27721 gccatttgcg gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg
    27781 tatgttgtag gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca
    27841 ttgaaaaaag ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc
    27901 tgcctgaatc acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg
    27961 cttttcggat ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga
    28021 gttccttatg cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa
    28081 gttttatttc tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta
    28141 aaaaaaggcg atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa
    28201 ttcgctgtta tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta
    28261 gataaagcgg ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct
    28321 ggaacagaat tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga
    28381 atggacggat tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc
    28441 acaatctctc cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta
    28501 actggtgttt atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat
    28561 atcgctgtca agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg
    28621 attgcagaag gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa
    28681 gcagcacaag catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg
    28741 aaaccttgaa ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat
    28801 acccatgaga tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc
    28861 ttctatttcc aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt
    28921 ttctatattt ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat
    28981 ttttatttgg ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac
    29041 atttttattt acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga
    29101 gaatttttca aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag
    29161 cggtcgtccc actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa
    29221 acgtggtatg atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc
    29281 tgatttccgc taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca
    29341 gggcacaaag atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg
    29401 ctcctccgac gatggtttct ttttcatcta agaccacata ccattgagga atactttcct
    29461 tattagcgat tccagtacga atactttcct catacacaac agcaggaact ccccattttg
    29521 atgaaaacca attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt
    29581 tatatgtaac caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag
    29641 tctttatgaa aattagcaat gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc
    29701 acttgttctc cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat
    29761 cgtagctacc ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc
    29821 tagtacacca cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac
    29881 ccatggaggc ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac
    29941 actaactaag accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat
    30001 aggtaaccca aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt
    30061 tgttacaacc atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc
    30121 taaagttgca cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat
    30181 aaacggtgcg ctatgtccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt
    30241 gattgtttgc atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa
    30301 tggtgtgata aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg
    30361 ttcttggacc actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat
    30421 tgcagcaact aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc
    30481 ttttgcaact gccggtggca caccatcagg cattttgatg atcaattttt catttccgct
    30541 taaacgtaca aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt
    30601 tgagctcata cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc
    30661 ggaaacaccg tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa
    30721 gacagaaaga atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc
    30781 accaccaaaa attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat
    30841 tactgctaat gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc
    30901 aactaaatgc cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa
    30961 tgcaacaaat ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc
    31021 gacgatacag atcaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat
    31081 gatcttgact gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg
    31141 tcagcatctc tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt
    31201 tttcatctgc taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc
    31261 tttttgcatt tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt
    31321 ccatttattg tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg
    31381 agtacttttt caccattcat catgccatag tcagccatat tgattacatc taaaggaatg
    31441 cctttaggtg ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa
    31501 agtaaaacgt ctacattttt agattctagt tgattatccg catctgaagc agatacagca
    31561 aagatgtctg cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga
    31621 cttgtactca ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg
    31681 ttcctttcta tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt
    31741 aaaaagttga tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa
    31801 agcgcacact aaacacacaa attaattttt attacctttt ttcttttaac gtgcacaaaa
    31861 aaagttgtga cacaaaactt gtcacaactt ttttcaaata aacggtattc aaaagcttgc
    31921 atctgcggta ataagtccaa ccaagttaaa aacataagat gttgttttta ctttgctatt
    31981 cttattactt gatgcaggac agctttttca caacctctca ataaacggtg ttcaatcagc
    32041 tgaccctggg tattagctca aaacgctgga ttcacaacct cttttatttc aaataaccag
    32101 taaatacaat gaacatctac aaaaaacttc tatttattta tagatttcgt caggcaaatc
    32161 caattctctc ttatcaatca aagtctgata ccacgaagca gattttttca atgttctgct
    32221 tcgttcatcg ttcaattcga ttcgaactaa tccatatcga ttcttaaatg cattcattgg
    32281 tgacacacag tcagtgaatg cccataacat atagccttcg caatttgcgc cttcttctgt
    32341 tgctttgatc aagtaatgca aatgctgtcc gataaattcg atacggtaat catcttgcac
    32401 gattcctttc tcatccatat actgttcttc atattctctt cccataccgt tttctgttat
    32461 cagccaaggg atattatgat aattttcttt tagatacatg gctatatcat acatgatttt
    32521 cggatagatt tcccatcccc tggaattatt cattttcttc ccaggtaaat caaagacttc
    32581 ataatatcgt tcaggatgga aaggtgtctc ttcattccat tgataacgag gggcgcgaac
    32641 gcgtttagga taatattgat tgattccaag gaaatctact gtatgatgct gaatcgtttc
    32701 taaatcctct gttgcaggat cgaaataaat cgattgtttt tgacacaact ccaggaactc
    32761 ttctgtgtag tatcctttta ccatcggatc aaaaaatact cgattataaa acaaatcata
    32821 catataagca gctttttggt cttctggaga attggatcga gcatagacca tttcaggatt
    32881 taaaatacag ccaatccttc caccataatt tccgctgcgg taaatctcta ctgctttagc
    32941 cgttgctaaa actttatgat aattccacat catccacttt tttgtgtttt gttcatgcgg
    33001 ccagcgaaca gcatccaaat agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa
    33061 ccagtttttt accctatcac cgtaacgatc aaaggcaatt tgagcatact tagcataaaa
    33121 atcaactact tttcttgaag accagccatc atatttttcc aaaagaaaag ctgggacttc
    33181 ataatgttcc aaacaaatca tcggttctac gtctgcttcg atcaaagagt cgatcatttc
    33241 acttatatgc tgagcatatt cttcatctac tatacagttc tcataatcag taaaaaaacg
    33301 agcccaattg atagaagtcc gataatgtgt cagatggatg gatttcatca gatccacatc
    33361 ctgctggtat tgttccataa aattagttgc tacggctggg ccatacccat tatgccaaac
    33421 aaaatgttca tttttatacc agctatctat aaacgaatcc tgtccttctt tttttccttt
    33481 ccaaccttcc gtttgccaag cagaagatgc agcaccaaga ataaaatttt ttggaatagt
    33541 aattttcatg atttcctcct gttttttaga tcccatcatt ttccacacga tttgctactt
    33601 ttacaaatgg cagatagatc aaaatagaaa caataataca aacaagttgc gtaataactg
    33661 caccgatatt tcctgctgtt gccagataag cattgacgat cggcggcatt ggccaaggga
    33721 ccattacaac tgctttcgcc gcaaatccta aagcggttgc aaaataacca atcgttccag
    33781 tgataagagg cgttaagata aacggaatag ctaaaattgg attcagcatg atcggcatac
    33841 caaaaataac tggttcattg atgttgaaga tcccaggagc aagggataac ttggtgattt
    33901 gtttcatatc ttcccgtttt ccaactaaca atactgcaat cagtaaaccg atcgtgactc
    33961 cagacccgcc catactcata tacatatccc aaaatggcat agtaataatg ttcggtactt
    34021 cttttccagc ttcaaaagct tccgtattta cagcaattgc cgctaaaagc aacggttccc
    34081 gtatcggctt caccatttga tttccatgga taccaatcac ccaaaagact tgcgcaataa
    34141 acatcaatag caaaataccc ggtaaccctt gtacgacacc ttctaatggg cgttgtacga
    34201 cattgtaaat gatatcataa aggtacattc ctgtaagttc tttgatgata aatcccgcag
    34261 ccgcgatacc cgatacagtc agaatcgtcg ggaataaagc agaaaaacta gcagaaacat
    34321 tcgttggcac ggtatcaggc attctcagct gtagtttctt ctgtttcctc aaccagcaat
    34381 aaagttcaat agaaaggatg gagaccaaca ttcccaaaaa taaccctttc gtatctgtat
    34441 attgtttagc caatacgttt tctactataa cagtctgatt ttcattcact aattcataaa
    34501 cagttggatt aacagataaa taagacatca ctgcgatgat accagggaat acaccatcca
    34561 ctttattcag cttggctaat tcgatcccta tcagaaaaac agcatagatg gtcaagaaac
    34621 ttagtgtgac atagctgatg gcagatgcga taggctttaa ttcttctaaa aaacgcaaga
    34681 aacttatctg tgctaagcca ttttccgagt caaagaccat tgctgaaaac aatgtgccaa
    34741 aagcacccgt aataattacc ggaatcaatg cagcaaatgc gttttttatc accatgatat
    34801 accttaaatt attgattctc gttgccacgt tccctaaaac attggctaat ttatccataa
    34861 aactcattcc agatcccctc attcctgctt cttctccttg acgtcatctc tttttgtcta
    34921 ttagtcagcg cttacatttc gatgaacaga aactatttac tcctcttttt taccaaatat
    34981 aaaacaaaaa ttcatctgat gtatttatca taaaatacat cagatgaact gtcaattagt
    35041 ttctttttct cctattattt tctgtaattc tctccgattt attgccatat ggacagtcat
    35101 tgcatcaaac gcagcaagtg cattccgcct ttcgatggcc tgaacgatct ctgtatgaat
    35161 atcgatcgtc tcgattttca gatgctggat atcataattt ttattaaaca aagcaattga
    35221 ttcattaatg attgggataa tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat
    35281 cgtatgaaaa tcgatgtcta attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc
    35341 aatcttttgc gttaattttt tcatttggac gatttgattc ttcgttgcat attccgctgc
    35401 caaacctgcc atcttaggct ccaataaata tctcagttca aataagtctc gtatcatccg
    35461 atcctgatct tcaatcaaag aaaaaccgag tgggtcattg gcaatccctc gcttatcact
    35521 gacaaatgtc cccgctcctt gccgaacttc aaggatattc ctggaagcta gagcccgcac
    35581 agcttctcgc accgtgcttc gccccacctc taaataatcg gacaattcat attcattcgg
    35641 cagtttatct cctacttgca actgcatttc ctttaaatat ctaaggatct tgtcagtagt
    35701 tacttctact agggttttat tttcttccaa accacatacc tccttttcat ctgatgtatt
    35761 ttttcgtaat tattataaca cgtttgaatc atctgatgat tttttgtata caaaattgat
    35821 taatccgttc atagacatac aaacgaagaa gatatttacc ataaaaaaga ctaggataaa
    35881 attcactcct agccttcgat catttttatt ttgaacgaca aaaaatatga tgttcaataa
    35941 cagacgatct tcattcgat
//
LOCUS       DAZKNH010000018        35882 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000018 DAZKNH010000000
VERSION     DAZKNH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.018"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACG8MG_000850"
     CDS             <1..1152
                     /locus_tag="ACG8MG_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFQ0374917.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8MG_000851"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8MG_000851"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFQ0374918.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACG8MG_000852"
     CDS             2728..3003
                     /locus_tag="ACG8MG_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFQ0374919.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACG8MG_000853"
     CDS             3009..3776
                     /locus_tag="ACG8MG_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFQ0374920.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACG8MG_000854"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACG8MG_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFQ0374921.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8MG_000855"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8MG_000855"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFQ0374922.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACG8MG_000856"
     CDS             7920..8222
                     /locus_tag="ACG8MG_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFQ0374923.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACG8MG_000857"
     CDS             8225..9388
                     /locus_tag="ACG8MG_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0374924.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACG8MG_000858"
     CDS             9414..12842
                     /locus_tag="ACG8MG_000858"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFQ0374925.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACG8MG_000859"
     CDS             12863..13987
                     /locus_tag="ACG8MG_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFQ0374926.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACG8MG_000860"
     CDS             13968..14414
                     /locus_tag="ACG8MG_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0374927.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACG8MG_000861"
     CDS             14398..14736
                     /locus_tag="ACG8MG_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFQ0374928.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8MG_000862"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8MG_000862"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFQ0374929.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8MG_000863"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8MG_000863"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFQ0374930.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACG8MG_000864"
     CDS             15893..16936
                     /locus_tag="ACG8MG_000864"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFQ0374931.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="ACG8MG_000865"
     CDS             17095..17823
                     /locus_tag="ACG8MG_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFQ0374932.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="ACG8MG_000866"
     CDS             17939..18184
                     /locus_tag="ACG8MG_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0374933.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="ACG8MG_000867"
     CDS             18181..18399
                     /locus_tag="ACG8MG_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFQ0374934.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="ACG8MG_000868"
     CDS             18535..19074
                     /locus_tag="ACG8MG_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFQ0374935.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="ACG8MG_000869"
     CDS             19067..21340
                     /locus_tag="ACG8MG_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFQ0374936.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="ACG8MG_000870"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="ACG8MG_000870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFQ0374937.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG8MG_000871"
     CDS             complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG8MG_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFQ0374938.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="ACG8MG_000872"
     CDS             complement(23167..23436)
                     /locus_tag="ACG8MG_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374939.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23698..23826)
                     /locus_tag="ACG8MG_000873"
     CDS             complement(23698..23826)
                     /locus_tag="ACG8MG_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374940.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(23906..24361)
                     /locus_tag="ACG8MG_000874"
     CDS             complement(23906..24361)
                     /locus_tag="ACG8MG_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFQ0374941.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="ACG8MG_000875"
     CDS             complement(24499..26427)
                     /locus_tag="ACG8MG_000875"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0374942.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="ACG8MG_000876"
     CDS             complement(26624..27457)
                     /locus_tag="ACG8MG_000876"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFQ0374943.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="ACG8MG_000877"
     CDS             complement(27649..28353)
                     /locus_tag="ACG8MG_000877"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0374944.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="ACG8MG_000878"
     CDS             complement(28376..29002)
                     /locus_tag="ACG8MG_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0374945.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="ACG8MG_000879"
     CDS             29328..31703
                     /locus_tag="ACG8MG_000879"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFQ0374946.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="ACG8MG_000880"
     CDS             complement(31727..32218)
                     /locus_tag="ACG8MG_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFQ0374947.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="ACG8MG_000881"
     CDS             32405..33325
                     /locus_tag="ACG8MG_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0374948.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="ACG8MG_000882"
     CDS             33479..33823
                     /locus_tag="ACG8MG_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFQ0374949.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="ACG8MG_000883"
     CDS             complement(34013..34513)
                     /locus_tag="ACG8MG_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0374950.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="ACG8MG_000884"
     tRNA            35041..35124
                     /locus_tag="ACG8MG_000884"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
     gene            complement(35448..>35882)
                     /locus_tag="ACG8MG_000885"
     CDS             complement(35448..>35882)
                     /locus_tag="ACG8MG_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0374951.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11868 a         6262 c         7404 g        10348 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa ataggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaata atgaatctta gtaccaaccg
    35461 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    35521 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    35581 tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    35641 gcgatccatt cttttgcaga gcttgtactt gctggagtag cagcagtttc tgttacagca
    35701 ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt ccgtttgaac cgtttcttgt
    35761 acaacaggtt gttcagcttg aacaggttca gcttggactg gtgcttcttg aacggctgct
    35821 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
    35881 ta
//
LOCUS       DAZKNH010000019        34962 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000019 DAZKNH010000000
VERSION     DAZKNH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.019"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACG8MG_000886"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACG8MG_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFQ0374952.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACG8MG_000887"
     CDS             complement(2020..2922)
                     /locus_tag="ACG8MG_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0374953.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACG8MG_000888"
     CDS             complement(2919..3611)
                     /locus_tag="ACG8MG_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFQ0374954.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACG8MG_000889"
     CDS             complement(3654..4295)
                     /locus_tag="ACG8MG_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFQ0374955.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="ACG8MG_000890"
     CDS             complement(4333..4647)
                     /locus_tag="ACG8MG_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374956.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACG8MG_000891"
     CDS             complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACG8MG_000891"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFQ0374957.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACG8MG_000892"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACG8MG_000892"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFQ0374958.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACG8MG_000893"
     CDS             complement(5984..6916)
                     /locus_tag="ACG8MG_000893"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFQ0374959.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACG8MG_000894"
     CDS             complement(6913..7689)
                     /locus_tag="ACG8MG_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFQ0374960.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACG8MG_000895"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACG8MG_000895"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFQ0374961.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACG8MG_000896"
     CDS             complement(10933..12009)
                     /locus_tag="ACG8MG_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFQ0374962.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACG8MG_000897"
     CDS             complement(12014..13294)
                     /locus_tag="ACG8MG_000897"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFQ0374963.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACG8MG_000898"
     CDS             complement(13313..14239)
                     /locus_tag="ACG8MG_000898"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFQ0374964.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACG8MG_000899"
     CDS             complement(14252..15535)
                     /locus_tag="ACG8MG_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFQ0374965.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACG8MG_000900"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACG8MG_000900"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFQ0374966.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACG8MG_000901"
     CDS             complement(16323..17234)
                     /locus_tag="ACG8MG_000901"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0374967.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACG8MG_000902"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACG8MG_000902"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFQ0374968.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACG8MG_000903"
     CDS             complement(17700..18398)
                     /locus_tag="ACG8MG_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFQ0374969.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACG8MG_000904"
     CDS             complement(18385..18771)
                     /locus_tag="ACG8MG_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374970.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACG8MG_000905"
     CDS             complement(18918..20588)
                     /locus_tag="ACG8MG_000905"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFQ0374971.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACG8MG_000906"
     CDS             20771..21004
                     /locus_tag="ACG8MG_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0374972.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACG8MG_000907"
     CDS             complement(21103..21534)
                     /locus_tag="ACG8MG_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFQ0374973.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="ACG8MG_000908"
     CDS             21613..22026
                     /locus_tag="ACG8MG_000908"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFQ0374974.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="ACG8MG_000909"
     CDS             complement(22064..23212)
                     /locus_tag="ACG8MG_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0374975.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="ACG8MG_000910"
     CDS             complement(23334..23930)
                     /locus_tag="ACG8MG_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0374976.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACG8MG_000911"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACG8MG_000911"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFQ0374977.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="ACG8MG_000912"
     CDS             27160..27591
                     /locus_tag="ACG8MG_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0374978.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="ACG8MG_000913"
     CDS             complement(27636..28985)
                     /locus_tag="ACG8MG_000913"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0374979.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="ACG8MG_000914"
     CDS             complement(29055..30218)
                     /locus_tag="ACG8MG_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0374980.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="ACG8MG_000915"
     CDS             30347..30670
                     /locus_tag="ACG8MG_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374981.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="ACG8MG_000916"
     CDS             complement(30853..32202)
                     /locus_tag="ACG8MG_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0374982.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="ACG8MG_000917"
     CDS             complement(32271..33224)
                     /locus_tag="ACG8MG_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFQ0374983.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="ACG8MG_000918"
     CDS             complement(33326..34516)
                     /locus_tag="ACG8MG_000918"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFQ0374984.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34716..>34962)
                     /locus_tag="ACG8MG_000919"
     CDS             complement(34716..>34962)
                     /locus_tag="ACG8MG_000919"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0374985.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         9952 a         7642 c         6120 g        11248 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatacaacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttccgaa ta
//
LOCUS       DAZKNH010000020        34772 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000020 DAZKNH010000000
VERSION     DAZKNH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.020"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(228..1115)
                     /locus_tag="ACG8MG_000920"
     CDS             complement(228..1115)
                     /locus_tag="ACG8MG_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0374986.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1112..1981)
                     /locus_tag="ACG8MG_000921"
     CDS             complement(1112..1981)
                     /locus_tag="ACG8MG_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0374987.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2118..3404)
                     /locus_tag="ACG8MG_000922"
     CDS             complement(2118..3404)
                     /locus_tag="ACG8MG_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0374988.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3677..4255
                     /locus_tag="ACG8MG_000923"
     CDS             3677..4255
                     /locus_tag="ACG8MG_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0374989.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4255..5184
                     /locus_tag="ACG8MG_000924"
     CDS             4255..5184
                     /locus_tag="ACG8MG_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0374990.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5181..6917
                     /locus_tag="ACG8MG_000925"
     CDS             5181..6917
                     /locus_tag="ACG8MG_000925"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0374991.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="ACG8MG_000926"
     CDS             complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="ACG8MG_000926"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFQ0374992.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7813..8322)
                     /locus_tag="ACG8MG_000927"
     CDS             complement(7813..8322)
                     /locus_tag="ACG8MG_000927"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFQ0374993.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="ACG8MG_000928"
     CDS             complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="ACG8MG_000928"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0374994.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9623..10714)
                     /locus_tag="ACG8MG_000929"
     CDS             complement(9623..10714)
                     /locus_tag="ACG8MG_000929"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFQ0374995.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="ACG8MG_000930"
     CDS             complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="ACG8MG_000930"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFQ0374996.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="ACG8MG_000931"
     CDS             complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="ACG8MG_000931"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFQ0374997.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="ACG8MG_000932"
     CDS             complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="ACG8MG_000932"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFQ0374998.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14677..15651)
                     /locus_tag="ACG8MG_000933"
     CDS             complement(14677..15651)
                     /locus_tag="ACG8MG_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFQ0374999.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15632..17515)
                     /locus_tag="ACG8MG_000934"
     CDS             complement(15632..17515)
                     /locus_tag="ACG8MG_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFQ0375000.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17533..19758)
                     /locus_tag="ACG8MG_000935"
     CDS             complement(17533..19758)
                     /locus_tag="ACG8MG_000935"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0375001.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19873..20907
                     /locus_tag="ACG8MG_000936"
     CDS             19873..20907
                     /locus_tag="ACG8MG_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0375002.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20974..21987)
                     /locus_tag="ACG8MG_000937"
     CDS             complement(20974..21987)
                     /locus_tag="ACG8MG_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0375003.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22065..22253)
                     /locus_tag="ACG8MG_000938"
     CDS             complement(22065..22253)
                     /locus_tag="ACG8MG_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFQ0375004.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(22693..22821)
                     /locus_tag="ACG8MG_000939"
     CDS             complement(22693..22821)
                     /locus_tag="ACG8MG_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0375005.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(22824..24197)
                     /gene="rlmD"
                     /locus_tag="ACG8MG_000940"
     CDS             complement(22824..24197)
                     /gene="rlmD"
                     /locus_tag="ACG8MG_000940"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0375006.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(24308..25363)
                     /locus_tag="ACG8MG_000941"
     CDS             complement(24308..25363)
                     /locus_tag="ACG8MG_000941"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0375007.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(25373..26803)
                     /gene="gatB"
                     /locus_tag="ACG8MG_000942"
     CDS             complement(25373..26803)
                     /gene="gatB"
                     /locus_tag="ACG8MG_000942"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFQ0375008.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(26800..28272)
                     /gene="gatA"
                     /locus_tag="ACG8MG_000943"
     CDS             complement(26800..28272)
                     /gene="gatA"
                     /locus_tag="ACG8MG_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFQ0375009.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(28272..28577)
                     /gene="gatC"
                     /locus_tag="ACG8MG_000944"
     CDS             complement(28272..28577)
                     /gene="gatC"
                     /locus_tag="ACG8MG_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFQ0375010.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(28683..30719)
                     /gene="ligA"
                     /locus_tag="ACG8MG_000945"
     CDS             complement(28683..30719)
                     /gene="ligA"
                     /locus_tag="ACG8MG_000945"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFQ0375011.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(30736..32970)
                     /gene="pcrA"
                     /locus_tag="ACG8MG_000946"
     CDS             complement(30736..32970)
                     /gene="pcrA"
                     /locus_tag="ACG8MG_000946"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFQ0375012.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            33125..34684
                     /locus_tag="ACG8MG_000947"
     CDS             33125..34684
                     /locus_tag="ACG8MG_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFQ0375013.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT         9517 a         7494 c         6337 g        11424 t
ORIGIN      
        1 tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa ttttttagca
       61 tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt cgagcacaaa
      121 cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc aaaagtatct
      181 ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta ttttacagag
      241 ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc agttggtaaa
      301 gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga agcacccata
      361 gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac taagggccat
      421 aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata ggtattttta
      481 gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc taatcgagcg
      541 gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa gatcaaaaaa
      601 gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa aaccgttccg
      661 ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg aaccatcatc
      721 gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat ctcaaatcca
      781 tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc gatcatcgct
      841 gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg aaaattgatg
      901 atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt attcgtcatt
      961 cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct taatgctgta
     1021 tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt gacgttgggg
     1081 cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct actttcgttt
     1141 ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct gccgcatatc
     1201 caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg gtggtcgttg
     1261 catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc aatgtcccga
     1321 tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc gtcactttga
     1381 taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc tccgaatcaa
     1441 tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc caagtgatcg
     1501 aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc ggtgtgtcaa
     1561 tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat agacttttca
     1621 tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg gttcgaaaca
     1681 accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt gctagaaaca
     1741 acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct tttttaaaag
     1801 ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata ttgacagggg
     1861 gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc atgattgata
     1921 tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct ttctgcttca
     1981 ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa agacaatatc
     2041 tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag cgaagaaatt
     2101 cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg tgtatctgct
     2161 agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc tacgactgct
     2221 tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt ccatgttgaa
     2281 aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata agcttcttgt
     2341 tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt gcccatcagt
     2401 tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc tccagctact
     2461 cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt ttgcagattt
     2521 tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc tgctccttgg
     2581 atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga ttgtatcgca
     2641 gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa aagagtggca
     2701 aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt ctccccatct
     2761 tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc aaggtcagaa
     2821 ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc tacttgaata
     2881 tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc tgccatcaga
     2941 tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta tattttatct
     3001 cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac aatagcagac
     3061 aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata atcttcaatc
     3121 aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt cagtttctga
     3181 acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt ctcataaact
     3241 ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca gacagtgact
     3301 ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa acctgatgct
     3361 gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat ccgctccttt
     3421 tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga aaacggtttc
     3481 tttattattc agatacttca aaaatttctc ctaaaataat aaaaaaatac aaatcaacta
     3541 tgacaaaagg aataatctta gcatctagac caaagatcga aatggtgagg tttctttaaa
     3601 ataaaaatat ccttgcattt tctcctagaa ataagtattt ttaaattaca agactattct
     3661 taaggcgggt aaaaaaatgc caatacgcgt aattcttatc gatgatgaac agacgatttt
     3721 agatggaatg aaacagttga tcaattggtc tgagcacggg tttcaattaa tagggacttt
     3781 ttccagtccg ctgaaagcgc ttgatttttc tttggtacat gcaatcgata tcgtggtcac
     3841 agatgtaatg atgcctgaat taaatggaat cgaattatcg cggctattaa aacagcaaca
     3901 acctgaaaca cagatcctta tcctctctag ctatgatgaa tttgatatgg ttaaagattc
     3961 ttttaaagaa ggcgtcgctg attatctttt aaagcctcga ttgagtcctg atttttttct
     4021 aaattcctta actgccgttt cacaaaaaat caaaaaaaag cccaacaaaa gcccaatcac
     4081 caatcgttat gagcagatct gcgagaatct ttcacagcag attgctggac aaactcgtcc
     4141 attgcttgac cctcttcttt ttgaacacac cttgtttttc tgttatatac cgaaaaacgg
     4201 ccattcagtt caaaacaagg ctctaaacaa aaacagtcga tcacccattc attgatggat
     4261 agcgacgatt cgtttactat ttatcctgtt tctacctttt cggaagaatc cggctatgta
     4321 atcaatactg attccaaaga tacacttagg aagtttgttc aaaagtttcg tcaagaagtt
     4381 tttccaggtg atttttttgt tgtttctgat tcttttgcaa tccaagatat caatgaaata
     4441 ttccaagaga tcagaaattg caccaaagga caacgtttct atcataaggg aaagaacctt
     4501 gcttatcagc atgaacttct tccgttatta accaaccacg agcagcaagc aagtagttat
     4561 ttaacaaaaa tcgttcataa agactacgct ttagctgtca aagaacttga gaatttcttc
     4621 acgacgatat tggaacagcc tattgatcca gcttatttga aacatgaggc aatcaatcgt
     4681 ttctatgcat tactcaacgc tttggctgac gagcatgttg ggaatgaaga actttctttg
     4741 ctcaagatcg cccttccttc tttattagcc gatgctgagt atttcgaaga tttttctatt
     4801 cttgtcaaag aaacacttga gaaactcacc cacttttctg caaatgcaaa aaaagacaat
     4861 ttcgatatct tgactttgat ctacgagttc gtccaggaaa attatgagaa agaaatcaat
     4921 ttacaacttt tatccgaaaa atatcatttt tcatatagct atctctcagc catctttact
     4981 gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag tccgtatcag taaagccaag
     5041 ctctttttga cggaaacacg gcttactttg acggaaatct gtgagaaagt cggctatacc
     5101 gaactgggtt atttctccag agtttttaaa gaagagacag gaatgacacc ttctcaatac
     5161 cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga gcatgaactc tttgccaaac
     5221 ttctgttcat tttgttcatc ggtctttttc tgcaagccat cgtcatttct ttgtttattt
     5281 atcatcgatc aagggatgcc tatatccagc tgttcaatca atcgaatgat gtggtactaa
     5341 aaaagattca atctgaattt gaaagtttga atgatacaat cgagaataca ttagccgctt
     5401 ttgattctaa tccagctgtc aaaagctatt tttccaatga tccagcccaa catatggaac
     5461 agcttcagca actaagaaca atccaaaaaa tgaatgactc tctttcaaaa atccatccaa
     5521 tgatcgatta cgatgtattg atctttggag aaaatggaag aacttttgtc ggaaacgaca
     5581 tgctcactgc cgtatctgcc gattcttttt ttcaatctgc gattgctcag agagtgaatg
     5641 aacgtgctgc tgatacacaa atgctttttg cacaccacgg attgactctt cgcgataaga
     5701 aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac attgaatcac gtttacggat
     5761 atgctgtttt atcgatctca tccaagcaac tagcgaatct ttttcaatct gtagtaaatc
     5821 ctgagatatc gaagatctcc ttcatgaacg aagaacagac gatcatcgca tcaaatgaag
     5881 aaaatacaat tgggagccga tcaatgattt tcaaaaaact aacagtaggt gaaacaacga
     5941 tttcaaatgg acagcagttg acccgtcttc ctctgtaccg ccaggattgt acattactca
     6001 gtcaagtcga tcttcattct ttatctaacc aaatgggcgt gatttttccg attattttgt
     6061 ataatatcgt gacacttgct tttgctggct cattggtatt tatgtatttg gatcgacaca
     6121 caaaatcgat ctaccgcttg attgattcgc taaaacggct agaggaagat ggcttaaaag
     6181 ctactgttcc tgtacaagga agccatgaag tccaattgct agggaatacg atcaatcaat
     6241 tgctaagtaa tgctcagcat cattatgaac agacgatcca agacgaaaag aagaaacgtt
     6301 cgcttgaaat ccaaactatg caagcacaga tacagcccca ttttatttat aatacgctga
     6361 ctagcttgaa atttttgatt tggcagaaaa aaacagatga agcagttcaa ggaatcgaca
     6421 gtttcattac tttactaaga agtacgattg ggaaaaaaga agaagtgatt ccagtcaagg
     6481 aagaaatcaa aagcgtacaa agttacatcg atcttttgag tatgcgatat ggcgaaagaa
     6541 tctcggctaa aataatgatt cctgatgaac tgcagtcatt atctatgccg aatatgatcc
     6601 ttcaaccaat tatcgaaaac gcctatcttc atgcttttca caataaaacg caaggattca
     6661 tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt cgaagtgatt gataacggcg
     6721 atggcttcga tactgaaaat accaaacaaa aaaatgatca tttttcagga atcggcatca
     6781 gccatgtaga tgagcggata cgcctaatgt atggcgatgc atatggctta acgatccaat
     6841 cagtgattgg gacaggaacg actgtaacga tttgtctacc gcttcttcct aaaaatcaat
     6901 cagaatatca aaaataaaga atagccaagc gctgattttt ttattttaag ttaaataaat
     6961 caatcagtat tcttgacttt catctacgcg tctcttctac tgtcagcact ggataacagc
     7021 ccaatatttg actttctcct ccgagcatct tgatttcttg gagcgcattc tctactaatg
     7081 tcattggacg gtccaacaac aaatcgatca cgaaaaaata ttctcccaag ctagttttca
     7141 atggtcggga ttcaatctta cttaagttga tttcccgcca gccaaaagct gccagcattt
     7201 tatgaagcat ccctggccga ttagcaggta gtgtcaagat gacactcatt tttactggag
     7261 ctgactgctg gctggtcatc tttcggtcac cgacgatcca aaaacgtgtc tggttcaact
     7321 cattatcttg gatattctca gctaagatct ccaatccaac atgctgtgct gtttctaatg
     7381 aagcaatcgc tgctgcgtct tctttcggat gttctgctac atacattgct gcagcagttg
     7441 ttgattctgt tgctactaaa ggaacattag gataatgcgt ttctaaaaac tgttgtgatt
     7501 gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt tgttgctgga ttgcctaaaa
     7561 gctgctgctt gataggaaga acgatttcac ttttaacttc gacttctgga tgtttggaaa
     7621 gaagatcgat tgtgtgatga acagctcctt ccaatgaatt ctctacagga acgacagcca
     7681 gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg gatggaagca tacgcagtca
     7741 attgttctgt aggaaatagc tgacaagccg cctgataagt gaatgaactt tctggcccta
     7801 gatagctgac gttcatatgc ttttgatcct ttctataatg gtatcgatga tcttttctgg
     7861 tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct tcataccaag aaagtcgttg
     7921 tgccaataat gcttttattt cttctcttgt ttttccaaga gctaaaggtc gaatagacgt
     7981 ttcatcttgt tggatacgat caactagaca gtccgcttct gcctctaagt aaagaacaat
     8041 cggctgtgtt tttaaaaaac gccgattttc tgcactttgg acaatccctc caccagtcgc
     8101 gataattttg tcttcatttg acagctttct taaaatatct gactccactt ttcgaaagct
     8161 tttttcaccg taatagtgaa aatattcggc aatcgacaat tggatatgtt cctcaatctt
     8221 tgtatctaaa tctacatact ctttttgtaa acattgagct aaccctttac cgattgttgt
     8281 ttttccagcg cccatgaaac caatcagcaa aatgctagcc atgagcttct cctcctgaca
     8341 caagttctgc taaatcgtca aaaaatgccg gataagagat tgaaacagct tcagctcctt
     8401 ctagttcgca aggtccatca gctatcaaag cagctacttg cagcatcata ccgattcgat
     8461 gatcgcctct gctggacact tttgcgccat gcagaggtgt cggcccatgg atgatcagac
     8521 cgtctgctgt cgcttttatc tttgctccca ttttctgtaa ttcttctgcc accgcatcaa
     8581 ttctgttggt ttcttttact tttaattctt ctgcgtcttt gatgatcgtg attccttctg
     8641 cttgagtagc cactaaagct agaatgggca attcatcaat cagacgcgga atgatttctc
     8701 caccaatcgt tgctttcttc aaatgactcg tcttgacact taaatcagcc gattggttct
     8761 gttcattgtg attcgtctgg gtaatcgctg cgcccatctc ctccaacaca tctaagatac
     8821 cagttcttgt tggattgacc ccgacatttt ttaacaacag ttggctttcc ggaacaagaa
     8881 gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc tggaacagtt atctgttgac
     8941 cagttaattt ttgcggtccg gtcactatga tcgttttatc ttccactgtg atccttccgc
     9001 caaattgacg aatcatttct tccgtatggt tacgggaacg ttctttttca acgattacgg
     9061 atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga tttgacttgc gcactggcta
     9121 caggcatatt gtatcggata ggtgataaag agcgggttcc tgtcacagtg attggtagat
     9181 actggtcatt ttcttctcct tgcaagtcag cgcccatctc gcgtaaaggt tccatcactc
     9241 gccccattgg acgtttagac aacgaagcat ccccgaacag agtagtcgta aaaggctgtc
     9301 cagccaaaat acccatcaat aaacgtatgg tcgtaccgga gtttcccatg tcaattgatt
     9361 cggttggttg gacaaatgag tggctaccgc gaccatgaat cttgatcacc tcttcctctt
     9421 cttcgatctc gacacctaat tgttggaagg catgtaaagt actcagacaa tcttctgcgc
     9481 gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc tccaaacatg atgctgcgat
     9541 gggaaattga tttatctgct ggtatcctaa cagtccctct taatccatgt atttgttgta
     9601 ataattgcat ttatccagcc tcctatttga tccagcaatg ataactggta ttagacataa
     9661 tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa actgatttgc agtattccta
     9721 aaatatcttc cctggtctct tggatcttca aattaatcaa agaaactccc gcttttccga
     9781 tcagtccagt tacttctgca ataacgccag gttcatctgg cacatcgata aataaatcaa
     9841 aaaaggcagg aatcgcgcct tgcttatgga caggcaactg gtctcgactt tcttttgccc
     9901 gattgaaaaa agaaaagatt tccgattggt tctcagtcat gatccattct gctatttgtg
     9961 tcatttcttg ttgccaatct gccaatcgtt ccaataatat ctgcttattg ctcaacaaaa
    10021 tatctgtcca catgacagga tctgaggaag cgatcctagt gatatctcga aagcctcctg
    10081 ctgccagttg tttggctcgc ggatactctt gattgaattg ttctgcctga ttgactaagc
    10141 cagctgccag aatatgtgga aaatgactta gcattcctgt tatacggtca tgttctcttg
    10201 ctgtaagagt tacatatttt gctttggttc cgcaaaatag ttcttgcagt ttttcaacat
    10261 cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc attttcaaat agtttagcat
    10321 ctgctgcctg tatacctgat ttatgagaac cagccattgg atgtccaccg acaaaccgaa
    10381 gatttttctg ctcagcggat gtcacgactt gctgcttcgt actgctgaca tccgtaatga
    10441 tgacgtgatc agacaagttg attctttcaa ggacatcaag ataagctaag gttgtacgga
    10501 cgggaacggc taataagata acgtctgctt gttctgcacc tgtttcaaaa tcacttgcaa
    10561 cgtaatcaac gatctgctgt tctactgctt gctcacgggt ttttgactga ctatcccaac
    10621 caactactat cagttctgga ttggtctgac gaatacaaag tgccatagaa gcaccaatca
    10681 gtccaagccc cacgataaaa acatgttgtg ccatcttatt tgcccccttt ttagtaatgt
    10741 ttcaagtagt cacggtactc cttcactgca gctttcaact caacaagcga atcacttggg
    10801 aattttgaca atagctctgt cgctaccaca gtggctacaa cattttccgc aacgacgcta
    10861 gctgcaggaa ctgctgtact atccgaacgt tctacacttg ctttgtaagg ttcttttgta
    10921 tcgatatcaa cactttgaag tggcttgtac aatgtaggaa tgggtttcat gaccccgcga
    10981 atgacaagtg gttcgccatt ggtcattccg ccttcgaagc cgcctagatt attggtacgt
    11041 cggaaatagc ctctttcttc ttcccatagg atctcatcca tcacttctga gcctggtaat
    11101 gaaccagaag tgaatccgac cccaaattct acgcctttaa aggcattgat actaaccaca
    11161 gcttgtgcga ttttggcatc tagcttcgtg tcccattgta cgtagcttcc taatccagct
    11221 ggaacgcctc caacgagcac ttctacaatt cctccgatcg tatctccatt tttctttgtc
    11281 tgatcgatca aatcttttat tctttgctca actgctggat caacaactcg tacttctgac
    11341 gcttcagaaa tttctttgat ttcttgaatc gagatatttc caggtatttc tgcttggatt
    11401 ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa gttcttccag aattttcttg
    11461 gcaattgcgc cgattgctac acgcatggtc gtctctcttg cagaggaccg ttcaagcaca
    11521 ttccgaagat ctcgatgatg gtatttcata ccgcctacca aatccgcgtg tcctggacgc
    11581 ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca cttctgctac agacatgaca
    11641 gttgtccaat ttttccaatc tttattttct accactaaag tgacaggtga accaattgtt
    11701 ttgccgtgac ggatgccaga cgtgatccga acacgatctt tttcgatctt catccttcct
    11761 cctctgccgt aaccagcttg tctgcgagcg agttcgtgat tgatttcttc ttctgacaga
    11821 ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt ctggtccatg ggattctccc
    11881 gcagtcatat aacgcatctt acagtcctcc taagtaatct ttgaatgtct ctgtgggaac
    11941 aggaacgatt tttgcttgcc caatcttttc tagcaagatg atattcaatt ggcttcctct
    12001 tgctttctta tcatgggtta tcgcgtggaa taattcatct ggatgactta gatcgtacgt
    12061 aatcggcaga tggaattttt gaagtatcct ttctaaagca gctgtcgatc cagttggaga
    12121 caagccgatt ttttctgcat gagaagtgat ggctaccatt ccaatagcta ctccttctcc
    12181 atgactgact accccatatc ctgctgtatt ttcaattgca tggccaattg tatgaccaaa
    12241 atttaaaata agtcgatttc cttggtcaaa gacatcttct tccactactt ttcgtttgat
    12301 ttccaacgta gcagtaatga ttggaacagc atgttccaga agatcttttt cgtctttcag
    12361 ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct gctgatttga tgatttcagc
    12421 gattccttct cgtaaacgac gtaccggcaa agtttttaga acctcaggat caatcaatac
    12481 accagctggc tggctaaatg ttccgacaag gtttttcgct ttgtttgtat tgactgctgt
    12541 cttcccgcca atactagaat ctacttgtgc aagtagcgta gtcggaattt gaacgaaatg
    12601 gatcccacgc atgaacgtcg aggccacgaa tgctgctaga tcacccacca ttcctccacc
    12661 aagtgcaagt atcccatcgc tgcgggtaaa atcattatct gctaaaaaat cgtataactc
    12721 agctgcttgt tctaaagatt tactggtctc acccgccggt acgattgaaa ggactggttc
    12781 aaaattagct tgtctcaatt ggaacatgat tttttctgca tatagtggag caacgtttgt
    12841 atcagtgatc acagctactt tttgaggtct ccaaatcgtt tgaagccatg atccagcctg
    12901 ttctagtgcg cctttttcaa tatgaatatc gtatttttgg ttatcaacat gtacagtaag
    12961 cattcgtttc acttctttct tttaaaaagc tggaattaat cgagtgtctg aatcttttcc
    13021 attgcttctt tcatgatcgc aacttctttc atcatgcgtg agtaagtctt ttcattcaac
    13081 gattggggac cgtctgacca agcatttgca ggatcaggat ggatctctac gatcatgcca
    13141 tctgctccgc ttgccacacc cgcacgagcc attggtggta caagttccca aattcctgta
    13201 ccatgacttg gatcaacaat cacagggaaa tgagttaatt ttttcatcaa tgggacagca
    13261 cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc gaatcccacg ttcgataaaa
    13321 atgacaggag atttgtcttc gactgcaatg tattctgcag cattaagcca ttcattgatc
    13381 gtaccggaaa ttccacgttt caatccaaca ggtttacctg ttttaccgac agcagatagc
    13441 agtttaaaat tttgcatatt acgtgcgcca atctgtagga tatcgctgta ctcagccacc
    13501 atatcgatat gcccttcatc catcacttca gtaatcactt tcattccaaa ttcatcagcc
    13561 gcttgacgga tgtatttcaa tccttcttct tctaaacctt ggaaagcata tggagaagta
    13621 cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc cggctttggc cattcgcgca
    13681 cattctctga tttggtctaa tccttcgata gaacaaggac cagccatcgt cacgaaactg
    13741 ccgtcaccaa tttttactcc gtctacatcc acgactgtat tgccaggatg gaattctcga
    13801 gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt ctactccttc atagcgtgta
    13861 aatgctcctt cttgtaaact tcgcgtatca ccttttagtc caatcactac tcgttctgtt
    13921 ccttgatcga ttgagacttt gaatccttct ttttcgatat gatcgacgac tttttttatt
    13981 tgttcttccg ttgcttcttt tttcataatt actatcattg tttttcccgc tttcttttgt
    14041 ttacttatct atcttggctg attttctcta ctttttcaag gtggttttac attaattcct
    14101 actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt ctttcctact tatgacccag
    14161 ataatcaaca aaaaaagatg tccctgtagg aaattgcctc tacagtgaac atcttcaacg
    14221 agccttaaaa agcgctgcaa aaatataaaa tagccactat agatgaattt ctctacgtgg
    14281 ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac aaacctggtt tgtttgtacc
    14341 cacgaagtca tgaatacaaa cctatctaaa atagctaaag taatacgtat tcagttttgt
    14401 aagttttcta gatttcataa aaaatcgctc caaactgatg taagaattgt acctagtata
    14461 gacataaaaa aatcgactgt caatcatttt tatgaaactt tttttaattt tctgatagtt
    14521 aactttgttt ttcagaaaaa taacaattgg ttttattgaa gaaaatagtc atgaacaaaa
    14581 gtatctgaga acttttttac tctcttgttc atgacatata gaataaagca gctttgtctt
    14641 gtaaactccg tttttcaaat aataatctaa agattgctac tgaataataa agccgatttc
    14701 atggtcctct tcgctgctga ttcggtaagg tgactcaaca tccgctcccc agctattgat
    14761 acccccgact cctcttactt ttgcaaaaat cgtaagaacc gttcgtcttt ctggcggtag
    14821 ttcttctata tgtgttgcct gttccagttc ttctgctttg aatggtaaag cggaaaaagc
    14881 aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct tcttgatctt tatccgtatt
    14941 atccaacgtt tgatttcttg ttagttctac ccatttcgtt tccatatgca tgccgcattc
    15001 ttggggaaca aggtatgggg aaacggggag tcctgccact ttataaatac cttcgacacc
    15061 accagccatc cgatccggat atgtttcccc tgataggcct tcatactcaa aaaattttgc
    15121 tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat tccggtaatc ctttttgtcc
    15181 ataataatgc atattgacaa gcattttacc ctctccagcg atttggtagg aaattgtcac
    15241 ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg atttcaaccg tttcagcgta
    15301 ttcattactt gaatattgat tatttactgg agcaatcggc aaatcgatct ttttattgtc
    15361 caccagtact tctatttctt tgacttttgg atacaatccg gctcctaacc acataccgga
    15421 tttaaatcca aatccgctac ctctgtcatt atcggtcgtt gctcgccaaa aaactggaag
    15481 tgtttcccgg tatagccatt cttttccgtt tttattgagc gattccagtc cgcctcttgt
    15541 ataagaaaaa atataatgcg tattccccga tttgacacct aatgtacagt cgccgaagac
    15601 gattgcaact ttttccgtat ccatatgact tttatttact gtatttttca taataatatc
    15661 ttacctcctg catggcgggt ttttcttgtc catctgcaaa aacgatccca ttccctgaga
    15721 attcatagtc acttggttta tcgtcaaagt caccgccata gcgcatcact tctctgcctg
    15781 tgattggatc agtcacttgg atcgcctgat ctttaaaatc ccatatatag cctccttggt
    15841 acatttcata acgatcaagc aacgtcatgt aagaatccat tccacctaac gaattcccca
    15901 tgtcatgcat atattcacat aagatgaacg gtttttccgc tttattttca aggtagtcaa
    15961 tgatctcttc tggtggcgca tacatgcagc tttttacatc tgaaatcgaa tcttcgaatg
    16021 ctggatttgg aaaattccct tcataatgga caagtcgatc ggggtctact tttttgaagt
    16081 aatcgttcat ttgtcgaatg ttttcttccg cataagattc atttccaaga gaccaaaaaa
    16141 gaatacttgg atggtttttt aatgtttcga aattcgtttt tgctcgatca agaacagccg
    16201 cctgccactg aggtactgca cctggtacgt tgtaagaagg ctcaaatgcg cccatttttt
    16261 gccatgagcc atgggattcg aggttcgttt ccgccatcac atggattcct gcttggtcgc
    16321 ataaataata ccaagtgatc tggttggggt aatgagatgt ccgtacgccg ttgatctttg
    16381 cattttttat gatagccaaa tctttttcca tatcttctat agtgatggcg cgtccttttt
    16441 tcggatgcca ctcatgtcga tttaccccgt tgataatcat ccgttttcca ttgaagtaga
    16501 tgattttatc tttgatttcg attttacgaa aaccgaagga atagtcaata tactccgtta
    16561 ctcttttttc ttcgtctatc gtctccaatt caagcttata taaattgggc tgaaaatgcc
    16621 cccacagtgc cacattttca aatccatcga tgcgaatcgt catttgctct tttaacggat
    16681 attgctgttg atagatttcc ttcccatcag gatctagtat cgctacattc agctgactgt
    16741 ttttggctac ttgctcaaaa gacaaatcta cttcaagttt gccattttga catttgtcat
    16801 ctaagcgagg tttgatccaa agatcttcta aatggttttg tcctcgagca actaagaaga
    16861 catctctgaa gatcccaaaa aatcggaaaa agtcctgatc ttccaagaat gcagctgtac
    16921 ttcttttgta aacctccaca gctagaatat tttcttcttc caaataaggt gttaaatcaa
    16981 attcagacgg agtgaacgaa tcttcggcat acccgataaa atgaccattc aaccaaataa
    17041 acattgcttg ttctacacca gcaaaatgaa tcgagatttg ttttcccttt agaccttctt
    17101 ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc tttagcagct gaaaagatcc
    17161 cttccgcctc tttctcaggg cttgtcgtga aagggggacg gcgaaataca tgtccctccc
    17221 acggatacat cgtattgatg taatggatct tgtcataatt tgctaattca atatgactag
    17281 gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt tttataaaat gtttttggtc
    17341 gtaccgaagg atttggcgaa taacaaaaac gccatgatcc atttaaagac tgtttaaggg
    17401 aggatgtggt ttctacttct tcccttgttg cataacaaac atgatcgcta tgtgcatttg
    17461 attggttcac tctaaaaact tgtggatcat ctaaccattt tatatttgct ttcatacttt
    17521 tttccttctc tctcataact ttttgaaatg aagcagttct gtttggaaat caccttgaaa
    17581 atcggggaag taaagtccaa ctgtcatcaa ttcggccccc gaaaatatcc gcttcgaggt
    17641 ctgttcctga taaagtgcct catcatctaa ccccatcaga tatacttcat ggaacgctgg
    17701 ttgagctgaa gctagtatcc tccctaaaaa cacaatagct tcatcttgtt taggagacac
    17761 aaacatccat gcggcaatat tttcttcaaa aggactcgcc aaacgataat aatggccata
    17821 ctggatcacc ggacgaatcg tttgatagga tataatttgg tcttttactt gctttttttc
    17881 ttcaggagac agctttgtca aatccagttc ataaccaaaa acagcgctca tagctgtttc
    17941 tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga tttggtacag ctgatacatg
    18001 agctgtgatg gacgaaatcg gataagcaag gcttgttcca tattggattt tcatacgctc
    18061 cactgcatcc gtgttatcac ttgtccatga ctgcggcata taataaataa acccggcatc
    18121 aaaccgtcca cctccaccag aacagccttc ccataagatt tccggataat cagttgtcaa
    18181 atgttcaagt agttcataaa gcccgagcat ataacgatgc gctacttctc cttgtctctg
    18241 tgggtcaagg gtaatcgaat agacatctga aagcgaacgg ttcatatccc atttgatgta
    18301 gtcaagagga atcgtatcta aaatctttct catttgtttc tcaatcgtct gccgaacatc
    18361 taaccttgta aaatccagta catgttgtga tctggaagca gaaggcattc tacctggctc
    18421 ctgcataaga aaatcaggat gtgttcgata cagttcagaa tcaaccgaga tcatttcagg
    18481 ttcaaaccat aaaccaaatt tcaatccttt gctatgagca taatctgcta tttcacgtaa
    18541 tccatttgtc aacttgcctt catactcgaa ccagtctcct aatgaagaat tgtccgcatc
    18601 tctttttcca aaccagccat catctaatac gaacatttcg atgcctagct cctttgcttc
    18661 atcaatgatc gccttgattt tttcactatt gaagtcaaag taagtggctt cccagttatt
    18721 gaccagaatc ggccgttcag caaattgatg acgacctctt gcgattcttt cacgtagcaa
    18781 ctcatggtgg atctgactca ttttgttcaa tccctgactt gagtaagaaa ggatcatttc
    18841 aggtgtttga aaagactggc ctgcattcaa ttcccacgaa aaacgatggc tgttgatccc
    18901 accgacgact ctcaattgat tgatctgatc tttttcaagt tcgaaagaat ggttgccgga
    18961 ataaacaaat tgaagtccga tcgcttctcc agtattttct gtcgtatgat gatgaactaa
    19021 agcaataaag ttattcatat ggtgactgct cgttcctcga cggctttcaa atacttttgt
    19081 tccagataaa acagattgcc gtgaaatctg tctttcattg acatgtgctc ctggcaaagc
    19141 aatgacttca tcaaatcgcc tagtatgggc aaagtctagc tggaaactag ccgctttttc
    19201 tagtttgatt gtttctccgg tttcatttct tatctttaca gatcgtgtga tcactgctct
    19261 atcagtaaat atggtataga acaaatcgaa ataaatcgct gtttctctat ccttaagtga
    19321 aatcaccaac gtttgtgcct ctgacttgtc catcacataa gtttgaggca agccttcgat
    19381 ctctggcttc ccatctgtaa tataatggct gtcatatctc agatccagta agtctgatcc
    19441 attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc atgctgccat gagaagagta
    19501 ttcttgcggt agtgtatctt tcgacagaga ccggtcagga tttaagggaa catttcctga
    19561 aaatccacga tctcttcggg gataattttt attgtctttg tactgcttga ctggtttgcc
    19621 aaaataaata tgcgccaata cattgctttc ttctatcttt atcagataag aaagaaatgt
    19681 atttcgtaaa tggaagactc ggctcttttc atcaaataag atgaaatttt tatctgtttt
    19741 tctatgcgta atatccatca tttcactccc aacaaaacgg aaaccgttta ctttttttta
    19801 taattcctgt atactgttta ctaaacaatt ttgtcaataa aaatttttta tttttttgag
    19861 gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa aggcaaatgt ctctcctgct
    19921 acagtttccc gtgtgttgaa taacgatcag acgatccatg tggctcagac tactcggcaa
    19981 cggatagtag aggcagctgc tgcgttggat tatgacatca gtactcgtaa atatatcaaa
    20041 aagagaatgc cttcgattgg tgtgatctct acgatcggca aagcgagcga agaacaggat
    20101 atctattaca aacatttgcg agtagggatg gaagaagaag ctcgacgtct gcatttaggg
    20161 atgaatcgaa tctataatct atctgataat cctaaacaat gggaagatct agatcagtta
    20221 ggtgcggtga tcgttgttgg gactgttacg aaagaatcga ttaccgcctt gttaaaacaa
    20281 aacatgcaaa tagtcgtcgt ggataaccct gacatccagc aagaggtcga tatggtgtat
    20341 gcagatcttg aacggatgac taaaaccgta ctggaattgt tcttagaaaa tggtcataaa
    20401 agaatagcct atatcggggg atataaagtc gatatggatg aattcggtgg gaagcagatt
    20461 tctataaatg aaaagaggat gcgtgcctat cagcatttta tgatcgatca tcacttagaa
    20521 aaatatatgt cttatcatct aggtggctgg acagaaaaac aaggtgaggt acttgcgact
    20581 aatctattag aaaatgaatc ccctcttcct actgccattc ttgtcgggag cgatccaatg
    20641 gcaattggtg tctatcgggc attgaaacaa aaaaatatcc aaattggaga agagattgtt
    20701 gtagcaagct ttgataatat cgagacaatc gcaaacctct cccctggctt gaccagtgtt
    20761 caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa tggcattgga gcgaattgac
    20821 ggtatacggg acgaaccaat cattttgact tatcctgcac gattgattgt tagggaaagt
    20881 tttatcccaa gaacaaaaag gtattgaaag agaggttgtg aaaagccgct tcgtttcttc
    20941 atactcaaaa cgaaacggct ttttcacaac ctcttactta tacttattca caaaatccaa
    21001 tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa tcaaattggt attcatgggt
    21061 aatctctttt tttgtatctg tataaaacaa ggattgcacg ggtaccttta attctgtcaa
    21121 ccgattttct aatgcttgac cttgctcctg gaatgagtat gcatttccgt cagttatata
    21181 cgttggcgga aattccttag aaacatggtc agccacacta gcttctttca attgctcact
    21241 cgttttccaa tcttttgttc caatcaacga ccacgcaacg gttttgacga aaaacttcat
    21301 aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa tcaacaggtc cgcaataaga
    21361 aatggttcct ttcagactat cttttggaag tagctgtttg attttcattt gtttagcata
    21421 cgcatcattc gtttgtatag ctgcatattg caatgcgatt tgtgaacctg cactatctcc
    21481 accaaaaaat aattgttcca tgtctagcaa tttgtcgtct ttttcttcag ctaataaatc
    21541 tttgatcagt tcattcactt gaagaacttg attagggtat tctgaatcag gcgctaattc
    21601 ataattcatc gcaaccactg cgatgttggc gtctgaaact aattttgtcg caaattcttt
    21661 gacacccgat ttatctccgc cgacgaatcc tccaccatgt acccaaaata gaacaggaac
    21721 aggccccttg ctgttttttg gatagtagat gtcataagta ttctctttat acttggactc
    21781 atacgtcaaa tctgtttttt tcgccacgtt tttttctgct tgatcgaacg tttttttatc
    21841 agtgatttga acagtatttg aaaacatatg gttgatcaag aatgctccag gtctggggga
    21901 aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga atgattccga agaaccatcc
    21961 gataaattta agaattttct ttttcataag gctccctcag tagttagtat taagcattct
    22021 gattatacca tagtgttatt tcctatcaag ctctcctaga tgatttaatg attttcccca
    22081 aacttcttct gcaatgcttt tgacaaaaga aattttttcc attgttcttc aaacaaaaca
    22141 ttttcttctt ctgttgaagc aaatccacat tgaggactta agcacaaacg atccaaaggc
    22201 agatatcgtg tcgcttcgta gatccggtca atcacttgct ggtgctcttc catttcgcca
    22261 tttttcgtta cagtcaaaat ctaagacaac taatttatct tcagagactt tctctaaaag
    22321 aacaaaactc ccagcacgtt cactatcgta ttctaaataa taggcacgga cattttcttg
    22381 agtaaaataa tgggtctgct acgtcgtcat cccccctgat gccgccaaaa tcgaataata
    22441 atttccgcgt caaacatgta tatttagtgt tgaatcattt gagaatatga aaataaaaat
    22501 ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa atctgagaca gaatgacgtt
    22561 cattctgacc cagatttgat tttgcacaac tcaacagtcc ttattggcaa atcgccaaaa
    22621 taatgtggac taactaaaaa gactaagaaa caataacaaa agttaacact tcttagtctt
    22681 tctttagcac gtctatttat taaagatttt agggcctttt ctcatgtttt tacgatcatt
    22741 cttaggtgtt tccatttgtt tggatcggcc tccaccttgt ttctttttga tcaattcttt
    22801 cattttttct ttttggtcca tttttattct cctttaacga atgaagcgac taattcgata
    22861 tgatgggttt gcgggaaaag atctacaggc tgaactttct tcacggcata acctgcttct
    22921 gcaaataact tagcatctct tgcaaaagtt gccggattac atgagatata gacgattcgt
    22981 tctggtgaca tttcgattgc tgattcaata aaatgatgat ctaatccttt tcgtggaggg
    23041 tcaactacga tcacagatgg tttgatccct ttttcttgcc attttttcat aactttttct
    23101 gcttttccga cttcataatg aacattttcg attttattat ttaaagcatt gaactgtgca
    23161 tcttcaattg cttctggaat aacttccatc ccatacacct tatccactct gtctgctaaa
    23221 gacaagccaa tcgtacctat tcctgaataa gcgtcgatca caatgtcttt tttatctaac
    23281 tcggcaaact cgatggctgt ttggtacaga tactctgctt gttgagtatt tacttgataa
    23341 aatgactttg ctgagattcg ataggttttt cctaatagct gatcttcgat atagtctctg
    23401 ccatataaaa ctttttcttt gtctcctaaa atcacatttg ttttttcttc attgatattt
    23461 tggatgaccg aaaccacttc tggcaattct tcatgaatag cttcagcaat ttccttggat
    23521 ttaaagaatt tttctttacg agtaactaaa acgaccatca tttcatggga ataatgacca
    23581 cgacggataa tgatgtgacg aagctgtcct tcattttttt cttcattata tcctcgtact
    23641 tggaaccgtt gcaagatatc tctgactact ttgattgctt gatcgatgac aggatcttgg
    23701 atataaaaat cttcgatagg gactagatca tggctattct ttttgtaaaa accggtggct
    23761 aactgtccat cgatacggcg aacagggatc tgtgctttat tacggtagcc gaatggttct
    23821 gccattccga ccgttggaag tacaggaact tctggcatct tagcaatctt cgccattaca
    23881 tgctctactt gtttttgttt gaagacaagc tgttgttcat acttcaaatg ggctaaaggt
    23941 gcgataccta tgcgaagtaa tgcattgttt tctacaggct gacgatcagg tgattgtgta
    24001 ataaatgttt cgacttttcc atatccaaac ttattgcctg cttttactac tagtatttct
    24061 acttcttctc cagggagtgc attttcgata aataatggat aaccatcgat ttttgctacc
    24121 cccattcctt cataactcaa gtcgacgatt tcgactgtat atcgttcgtt tttctttact
    24181 atttgctgtt ctgtcatatt ttgctccttt aagcctattt ttcaatccta ctgaactcta
    24241 ctataaatca gctaattaca caactaaaac cggcactgac cttactacgt tcagcaccgg
    24301 cttttgatta attcttttga ttaggtgttt cgtctgctat ttttccgtca ccgtcgatat
    24361 cttcatccgt taaacgttcg acttctttta cgaattcttt gctgacaacc tctaattctt
    24421 cttcatctgt acctgtgatt gcttcatcag gaatctgatc ggtattggca aagaactcaa
    24481 tgtgttgatg aagattttgg aaatgcatcg gtgcttcccc gccgtattca ccatctaagt
    24541 tgatcattac tggtcgattg atatcatctt ctgtttcaac gaacaaactg cttgttttgg
    24601 tatagattat tcttggatca tcgacatgtc gaccgccatt cagcatgagt gctgcaatat
    24661 gcaagatctc aaagatattt gctgtcttca caatgattaa agaaaacttc ccatcatcca
    24721 atttggcatc tggcgcgatc tgttcgaatc ctccaacaga gttcgtcagc cctaggaaaa
    24781 acatcgaagc atttccgata tattcccctt catcatactt caagcgcatt ttgattggct
    24841 ttactcgagg gagcatctct gctcctttcg caagataagc aagataacca aaaatactct
    24901 ttagttctga tggaacttca taagtcaatt ctgtcaaata gccacctgca gcgatattga
    24961 tgaagtaact gttatcagaa aggccgatgt ccatttttac ggtttggtgt ttttttatca
    25021 cttctgctgc tgctttaata ttatctctag gaatttttaa tgctcgagca tagtcgttcg
    25081 ttgtaccagc tgggatgatg gccattttag gacggtgttc aagcggagcg attccattaa
    25141 ccacttcgtt gattgtgcca tcgccaccag ctgccactac tagatcaaaa cctagttctg
    25201 cgactctaca tgcttcattc ttggctgaat ctggttctgg tgtagttgca taggcgctgg
    25261 cttcaaatcc tgcttcttct aatgcctgta ggatatccgc caaatttttt ttcaataatt
    25321 cttttcccga ggtggggtta taaattacgc gtgctttttt cattttcagc ctctatcgtt
    25381 tcgctaattc atctttaagt agcttattca cgacaccagg atttgcttgt cctttagttg
    25441 ctttcatgat ttgaccgact aggaagccaa ccgcacgatc ttttccattt ttgaaatcat
    25501 cgacagattg ttggttgtta tccaaaattt cattaatgat tggcagtagt ttgttcggat
    25561 cagataattg cacccaacca tttttttcaa ctacttcttg ggcttctccg ccatttgtga
    25621 tcaattcacg gaagactttt ttggcgattt ttgagctgat cgttccatcg gcgatcaaac
    25681 gaatcattcc tgctaagttc tcaggtgtca attttgtctc agctaattcc agtttctcac
    25741 tgttcaagta agcagaaact tcacccatca accagttaga tgcttgcttg gcatctgcac
    25801 cattttccaa aacagcttca aaaaaatcag acatttcttt tgtaagtgtc aataccattg
    25861 cgtcatactc tggaagtcca aactcacgga tatagcgttc gcgacgagaa gctggcatct
    25921 caggcaaact agcttttact ttttgaatcc attcgtcatc gatcacgaat ttcgggatat
    25981 caggctctgg gaagtaacga taatcactag acccttcttt gacacgcatc aaaactgttt
    26041 tattcgttgt ttcgtcaaaa cggcgtgttt cttgttggat ttctcctcct gaaagaagaa
    26101 cttttgcttg acgtttttct tcaaaagcta aacctttttt cacgaaagat aaagagttca
    26161 agtttttcaa ttctgctttc gtaccgaact cttcttgtcc ataagggcga agagaaatat
    26221 tggcatcaca gcgcatagag ccttcttcca ttttcacatc gctgacttct gtaaattgga
    26281 taattgatcg caatgcttct agataagcgt atgcttcttc tggtgaacgc atatcagctt
    26341 cagaaacgat ttcgatcaaa ggcgtgcctt gtcggttcaa atcaacgtaa gaataaccgc
    26401 caataccatg catgtttttt ccagcatctt cttctaaatg gacacgttcg atccggattt
    26461 ttttcttttt accttctact tcgatttcga tccatccgtc atgaccgatt ggttgatcaa
    26521 attgggaaat ttgatatgct tttggattat ctgggtaaaa gtaatttttc cggtcaaaat
    26581 gagtatcttt agagatttca cagtttaatg ccaaagctgc tttcatcccg aattccagcg
    26641 cagctttatt cattactggc agcacgcctg gataacccca gtcgatcacg tttgtattgc
    26701 tgtttggttc tgcaccaaaa tgagctggcg ctggagagaa aatttttgag ttggttttta
    26761 actcgacgtg gacttctagt ccaatgactg tttcaaaatt cattcgtctt tccctcctaa
    26821 aatagctggt tgttgtttat ggaattctgt tgcttgttca aacgcatatg ccgcttgata
    26881 catcgttttt tcatcaaaat aattaccgat gatctgcaat ccaactggta atccttctga
    26941 taaacctgcc ggtacagaca tacctggaag tccagccaag ttgacaggga tcgttaagat
    27001 gtcactcatg tacattgtga ttggatcgtt aatgttctca ccgattccga atgcaactgt
    27061 tggtgaagat ggcccgatga tcaagtcata atctgcaaag actttttcaa aatctcgttt
    27121 gatcaatgta cggacttgtc ctgctttttt gaagtaagcg tcataataac ccgcgcttaa
    27181 ggagaatgta cctaacatga tacggcgttt tacttcttca ccgaatcctt ctgaacgtga
    27241 gttgacatat acatcatcta atgatttcac attttcagag cgataaccgt agcggatacc
    27301 atcgaatcgt tgcaaattgg aacttgcttc tgatgaggca atgatataat aaacagccac
    27361 accgtatttt gaatgaggca agctgacttc ttccacaaca gcacctaatg aacggaatgt
    27421 ttctgctgct ttcaatacag cttcttttac gccaggggcc acaccttcgc ctaagtattc
    27481 tttaggtaaa gcaatcttca ttcctttgat gtcgcctgtc agtccctctg caaaatcagg
    27541 aactgaaacg cctgaagaag taccgtcttt ttcgtcataa ccgctgatag ctgtcaatgc
    27601 taatgcatta tctttcaccg tacgggtcat tggtccgatt tggtcaagag atgaagcaaa
    27661 ggcaatcaaa ccaaaacgtg agacacggcc ataagtaggt ttcatcccta cgatgccgtt
    27721 aaaagctgct ggttggcgga tacttccccc agtatcacta cctaaagaaa ctggaatttg
    27781 tccagctgca actgctgctg cagaaccacc agaagagcca ccaggtactt ttgttgggtc
    27841 ccaagcattt tttgttttct tgaaataaga agtttctgta cttccaccca tagcaaattc
    27901 atccatgttt agcttaccaa cagggatcat atctgcttga tagattttat ccatgaccgt
    27961 tgcatcataa attggttcaa aattgtaaag gatcttagat gcagcggttg tcaagatatc
    28021 ttttgttacg atattatcct tgataccaat tggaatacca gcaaggacgt tgctttcatc
    28081 aatccctttt tcatctaaag cttttgcctg ttccaatgct ttttcttcgt tcaatgtaat
    28141 gaatgcctcg acctcaggct ctgtttcttt gattcgttca aatgttgttt ttgtcaaatc
    28201 ttgtacagtg atttctttcg atacaagcat atgatgcagt tcttctagag aacgattgtg
    28261 taattctgtc attatgcgcc agcctcccca ttatcgatga ttgcgggtac tttgataaag
    28321 ccgtcttctg tttccggcac atttttcaat aattcatcgc ggttccatcc agcttgcgcc
    28381 cgatcttgtc gcatcacgtt tactgtttca accacattcg acgtaaatgg aacaccttcc
    28441 gtatctactt cttccagcat ttcaaccata tcgatgattt tacctaattg gtcggtaaaa
    28501 cttgcaagtt cttcatctgc gaacgcaagt tttgctaatt tggcaacgtg tttcacttgt
    28561 tcttcactaa tcgccatact accccttctt tctaaacgag ttaaaaactt tgctctctat
    28621 tttaacacaa gagtgccgtg tctgaaattg tttattgaaa tttgtctgaa aaaactcatt
    28681 tttcattctt ctactgagcg acttttagcc aatgcatctg ccatttgatc ttctgtccaa
    28741 acttcgattc ctaattcttg tgctttggtc agcttgctgc cagcatcttc tcctgctacc
    28801 acgatatccg tttttttcga tacgctacca gtcactttcc cgccaagatt ttcgatcgtc
    28861 tcttttgctt cttcacgagt aaatcgtgtt agtttaccag taaggactac tgttttttct
    28921 ttaaaaggag actcgacttc ttgcaattgg gcatttcgaa gccctttgta ttcgaaattg
    28981 acgcctgctt gttttaattc attcatcaat tcatggactt cttcgttact gaaataagtg
    29041 actacactgt cggcaatgat atcaccgatc gtatcaagcg caataatcga ctcatagtca
    29101 cttttactca atgtttctag atcaccgaaa tgttctgcta aaatttttgc tgctttcgct
    29161 ccgacatgtc ggattcctaa cccgaaaatc aaccgctcga ccgaattatc tttgctgttg
    29221 tcgattgctg tcaaaatatt atttgctgat ttttctttta ttttgtccaa cgtcaataac
    29281 tcttcttctg tcaatttgta aagatcagca acatctgcta ctagtttttt atcatacatc
    29341 tgttctagta cgcgcggccc caacccatcg atattcatcg catttcttga aacaaagtgg
    29401 ttcaaacctt ctttcatttg agcagggcat ttaggattga tgcaccgtaa ggctacttct
    29461 tcatccaaat ggacaagttc acttccacaa actggacaat gtgtaggcaa ttggtattct
    29521 tcgctgtctt taggtcgttt atctaacacg acttgcgaaa cttctgggat gatgtctccc
    29581 gctttataaa tcagaactgt atctttcaag cggatatcct tcaacttaat ataatcaccg
    29641 ttatgcaggc tggcacggct tactgtcgtt cctgctactc ggactggtgt catgatagct
    29701 gttggcgtta ctacccctgt tcgaccgact gtccattcga tgttttcgat tagcgtttca
    29761 acttcttctg gcggaaactt gtaagctgct gcccatctcg gtgctttgat agtaaaacca
    29821 agttgatctt gtagcgagaa ttcattcact ttgatgacga ttccatcgat ttcatagggc
    29881 aaatccacac gtttgtcatg atattcttca atgtatgacc acacttcatc aatcgaatga
    29941 catagacgtt tttcccgatt cgtgtggaat ccaatttttt ctagctcttc tagcgcatca
    30001 tactgggttt ttgcttgcat aggaccgaaa tcagctactg tgtataaaaa agtgtccaga
    30061 tttcttttcg ctgtgatttt agaatctaac tgacgcaaag aaccagctgc ggcgttccga
    30121 ggattcgcaa agatatcttt tccctctgct tctctgtctt ggttcaattg gacaaatgac
    30181 cgttttggca tgaaacattc gcctcttact tcgatgttca ttggttcttt taatttgatt
    30241 gggaccgacc ggacagtttt taggttttca gtgatgtttt ctcctacagt tccatccccg
    30301 cgagtcgcac ctctcacgaa atttccatct tcataacgca aagaaacgga aagtccatct
    30361 atttttagtt cacagcaata gtcaactaca cgtcccactg ctttttgtac acgttgatca
    30421 aaagctatca gctcttcttt gctgaaaacg tcgttcaaac tgtaaagcgg aatatcatga
    30481 gtgacttttt caaaaccttc taatactttt ccgcctactc gttgagtggg agaatcagac
    30541 gtgatcaaat caggatattg ttcctcgatt gctactaatt ctgcgtattc tttatcataa
    30601 acatagtctt ccactgttgg tttatcttct acgtagtatt cgcgagacca ttggttcagt
    30661 cttgtacgaa gttcttctgc tcgtttagca gcttcgtcaa tcgtcatttc atgttccatc
    30721 atttaccctc cactactaag ctttttcaat tggcgcaaag gcagccaaaa gacgttttac
    30781 tccttgactt gggaaagcca catctagttc tagatccttc gacgtcccgc ttactcggac
    30841 gactgttcct agaccccatt ttttgtgttt gactttatct ccaggtttcc agctgttatt
    30901 ttcccctcca gtagcacttt tagagggaac aaccgctgtc tctggctgtt tatagctata
    30961 tgctgttttt ttagcgaata caggcgtttt tttaggcgta ggacgcatgc cttcgatttc
    31021 cagtaactcc tgatcgattt cttctacaaa acgagaaggt cggttatatt gcgtccgtcc
    31081 atataatgtt ctggagaaag catttgttaa atacaacgct tcttctgctc gcgtgatccc
    31141 cacatatgcg agacggcgtt cttcctccag ttcgctttct tccatcaatg ctcgagataa
    31201 aggaaaaacc ccctcttcta gaccaatcag gaatacaact ggaaattcta atcctttggc
    31261 tgcatgcaaa gtcatcaatg tcacttggga agcatcttct tctaaattgt caatgtcgga
    31321 aacaagtgcc agatcattta agaagaccgt tagtttttct tctggtgcat cagcgtcttc
    31381 ttcattttgc tgttcgaatt gtttgtcgaa ttcttgtgtg accgttaaaa attcctccaa
    31441 gttttcgatg cgtgcttgtg cttctaatgt attttggatt tttaagtctt ctagatatcc
    31501 gctacgatcc aaaacctctt tcgtcaattc tgtcactgtc aagtatggga tcatctgagt
    31561 gacttcttgg atcatctctc caaatgcacc taattgctgt cctgcttttc caccgatatt
    31621 tgccagatcc acattttgcg ctgcttcaag cagtggccat tcatgaagag aagcaaaact
    31681 gcgtagtttt tcgatagatc ctggtccgat gcctcgtttg ggcgaattaa cgatacgctc
    31741 aaaactgatt gaatcttgag gatttaccag cacattcaaa tacgctaaaa tatctttgat
    31801 ttctttacga tcgtagaact tgtgccctcc aaccatttta taaggaatat ttgctttcaa
    31861 cagagtttct tccattacac gagattgtgc gttcgtccgg tacaagatgg caaagtcgcc
    31921 gtaattccga tgattgcttc gaatctcttc ttgcatccgg tctacgataa aacgggtttc
    31981 gtctcgttca ttatctgcac ggtagtaagt gatcttgttt ccttctttgt tttctgtcca
    32041 caagtttttt ggttttcgat tgctattgtt ttcaatgact tggtttgctg cgctaaggat
    32101 gtttttcgtg gagcgatagt tttgttctag caaaattact gcagcatctg gataatcttt
    32161 ttcaaaatcc aagatgttct gcatatctgc accgcgccag ccgtaaatac tttgatctgc
    32221 atctcccacg acgcacaggt tcctaaatct acctgccaat aaattgacta gcgtatactg
    32281 tgcgtgattt gtatcttggt actcatctac atgaatataa tggaatttgt tttgataata
    32341 cgtgagtgaa tcaggatgtt cttcaaataa tcgaatcgta ttcatgatca ggtcgtcaaa
    32401 gtccatacat tgattatatt gaagctcttt ttggtaagca gcataacatt ttgccgcaat
    32461 ctcttcgtaa taactgcctt gcatttttgc aaaatcctga ggggtctgca ggctgttttt
    32521 tgcttgagag attgttccta atatacttcg aggatcatat tttttcggat caatgttcag
    32581 ttcttttagt atccgtttca tcaaggtcag ctgttcagaa gaatcgatga ttgtaaagtt
    32641 ccggttatac ccgataaaat caacatctct tcttaaaata cgcacacaca tcgagtgaaa
    32701 agtcgatacc cagacatctt cgcctccaga tgcgagaatc gcattgacac gttctttcat
    32761 ttcttttgca gccttattgg taaaagtaat cgctaggatg ttccaaggat tgacttcttt
    32821 ttcctcaatc aaataggcaa tgcggtgtgt cagcactctc gtctttccac ttcctgcacc
    32881 tgccattagc agtaatggtc cgtcagtgtg aagaacagct tctttctgac gggggttcat
    32941 cccgtttaat aattcagctt tggatgacat taacaacatc ctttctttta tcaatcttgt
    33001 taattatagc attttcttgg ctttcattaa actagctagt tcaaaatcat ttgtttcttt
    33061 ttatggattt ttccttgtca attcgttgat ttcgcgctaa attaaaagaa agcgaggcgt
    33121 ttgaatggaa aaaaaaggaa aaatcaagaa attagataag actcagatat attttattct
    33181 aaaaggaata tttgttggac tgctgacagg aatcgtcgtt agcctgtttc gattatctat
    33241 cgaaaagcta agcgatttag ttcgaagtat ctatgaaatg agtcgggaac agccgattta
    33301 cttaattggc ctagctattt gctgcatcct tgcagcattt ttcgtgggtt atctggtcaa
    33361 aaatgaaccg gatatcaagg gaagcgggat tccacaagta gagggacagc ttcgcgggga
    33421 attatcaatg aactggttct ctgttttatg gaaaaagttc attggcggtg tactttcagt
    33481 tggtgcgggc ctttttctag gtagagaagg accttctata cagcttggcg catccattgg
    33541 acaaggagct ggacaattgt ttcggagtcc atcttcagaa gaaaagattt taatttctag
    33601 cggtgcaagt agcggacttg cagcagcatt caatgctcct atcgcaggat tactctttgt
    33661 tttagaagaa atccatcact ccttttctcc acttgtttgg ctgacatcat ttgcttcagc
    33721 aatcactgct aacttcgtct ctctttactt tttcggcttg agacctgttt tatatctagg
    33781 tgaccttcct tctttgccgt tgaaatatta tggttcctta gtgatcttag gagtacttct
    33841 aggcatctta ggttttattt atcagaatgt cttgctggct ttgccaaaat tttacaaccg
    33901 attgccttta ccagcttatt ttcacgggtt cgttcctttc ttgctgattt tgccaattgg
    33961 ttatttatgg cctagtactc taggcggagg caatcaaatc gttttagctt ttggacatgc
    34021 ttccttgcca atcattatga ttatcggttt gtttgttctt cgattcgttt tttccatgat
    34081 ttcttacggt gggggtcttc caggggggat tttccttccg atattgacat tgggggcttt
    34141 aatcggtagc ttttatggaa acattatggt tgatttcggg atggatccaa tctacatcaa
    34201 ggattttgtc gttattgcca tggctggtta tttcacagcg attggaaaag caccgctgac
    34261 agcaattatt ttagtgacag aaatggttgg ctctcttaat catctgatgc ctttaggatt
    34321 ggctgctctt gttgcttata tcgtcaatga ccttctcggc ggtaatccaa tttacgaatc
    34381 attattggaa cgtctacttg gaaatactca gaccgcctca ctaaaaggtg caaggacaac
    34441 ttttgatttt ccagtaacgg cagagagttc tttagacgga ttaatggtac gagacttcaa
    34501 ttggcctaaa aatatgctgc taatctctat acgcagagga aatcaggaaa tcttgactca
    34561 cggtgatacg atcatgaatg taggagatat tttggttatc ttgactgatg aagcaaactt
    34621 gccaagaatc aagcaggaaa tcgaggctaa atcttctatc acaaatttga agcaatcatt
    34681 ttaacgaaca tggaaaaact attttacaac aactattcat ctacactact tgattctata
    34741 tgattctata aaaaaagaag aggctgggaa aa
//
LOCUS       DAZKNH010000021        34532 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000021 DAZKNH010000000
VERSION     DAZKNH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.021"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(245..2137)
                     /locus_tag="ACG8MG_000948"
     CDS             complement(245..2137)
                     /locus_tag="ACG8MG_000948"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375014.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2134..3861)
                     /locus_tag="ACG8MG_000949"
     CDS             complement(2134..3861)
                     /locus_tag="ACG8MG_000949"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375015.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3877..4527)
                     /locus_tag="ACG8MG_000950"
     CDS             complement(3877..4527)
                     /locus_tag="ACG8MG_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFQ0375016.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4731..5027
                     /locus_tag="ACG8MG_000951"
     CDS             4731..5027
                     /locus_tag="ACG8MG_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0375017.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5199..5819
                     /locus_tag="ACG8MG_000952"
     CDS             5199..5819
                     /locus_tag="ACG8MG_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0375018.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5911..7077)
                     /locus_tag="ACG8MG_000953"
     CDS             complement(5911..7077)
                     /locus_tag="ACG8MG_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0375019.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7089..7442)
                     /locus_tag="ACG8MG_000954"
     CDS             complement(7089..7442)
                     /locus_tag="ACG8MG_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0375020.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7570..8592
                     /locus_tag="ACG8MG_000955"
     CDS             7570..8592
                     /locus_tag="ACG8MG_000955"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFQ0375021.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8611..8772
                     /locus_tag="ACG8MG_000956"
     CDS             8611..8772
                     /locus_tag="ACG8MG_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375022.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9031..9465)
                     /locus_tag="ACG8MG_000957"
     CDS             complement(9031..9465)
                     /locus_tag="ACG8MG_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375023.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9575..11434)
                     /locus_tag="ACG8MG_000958"
     CDS             complement(9575..11434)
                     /locus_tag="ACG8MG_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0375024.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11435..11575
                     /locus_tag="ACG8MG_000959"
     CDS             11435..11575
                     /locus_tag="ACG8MG_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0375025.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11552..12712)
                     /locus_tag="ACG8MG_000960"
     CDS             complement(11552..12712)
                     /locus_tag="ACG8MG_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0375026.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13019..14443)
                     /gene="araA"
                     /locus_tag="ACG8MG_000961"
     CDS             complement(13019..14443)
                     /gene="araA"
                     /locus_tag="ACG8MG_000961"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFQ0375027.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14459..15157)
                     /locus_tag="ACG8MG_000962"
     CDS             complement(14459..15157)
                     /locus_tag="ACG8MG_000962"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0375028.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15114..16775)
                     /locus_tag="ACG8MG_000963"
     CDS             complement(15114..16775)
                     /locus_tag="ACG8MG_000963"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFQ0375029.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16993..18393
                     /locus_tag="ACG8MG_000964"
     CDS             16993..18393
                     /locus_tag="ACG8MG_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFQ0375030.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18645..19724)
                     /locus_tag="ACG8MG_000965"
     CDS             complement(18645..19724)
                     /locus_tag="ACG8MG_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0375031.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19810..20769)
                     /locus_tag="ACG8MG_000966"
     CDS             complement(19810..20769)
                     /locus_tag="ACG8MG_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0375032.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20781..22292)
                     /locus_tag="ACG8MG_000967"
     CDS             complement(20781..22292)
                     /locus_tag="ACG8MG_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0375033.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22314..22877)
                     /locus_tag="ACG8MG_000968"
     CDS             complement(22314..22877)
                     /locus_tag="ACG8MG_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0375034.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22883..23746)
                     /locus_tag="ACG8MG_000969"
     CDS             complement(22883..23746)
                     /locus_tag="ACG8MG_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0375035.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23760..24656)
                     /locus_tag="ACG8MG_000970"
     CDS             complement(23760..24656)
                     /locus_tag="ACG8MG_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0375036.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24831..26120)
                     /locus_tag="ACG8MG_000971"
     CDS             complement(24831..26120)
                     /locus_tag="ACG8MG_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0375037.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26092..27609)
                     /locus_tag="ACG8MG_000972"
     CDS             complement(26092..27609)
                     /locus_tag="ACG8MG_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFQ0375038.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27807..28697
                     /locus_tag="ACG8MG_000973"
     CDS             27807..28697
                     /locus_tag="ACG8MG_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFQ0375039.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28770..29594)
                     /gene="nadE"
                     /locus_tag="ACG8MG_000974"
     CDS             complement(28770..29594)
                     /gene="nadE"
                     /locus_tag="ACG8MG_000974"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFQ0375040.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29606..31078)
                     /locus_tag="ACG8MG_000975"
     CDS             complement(29606..31078)
                     /locus_tag="ACG8MG_000975"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFQ0375041.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31715..32077)
                     /locus_tag="ACG8MG_000976"
     CDS             complement(31715..32077)
                     /locus_tag="ACG8MG_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0375042.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32300..33052
                     /locus_tag="ACG8MG_000977"
     CDS             32300..33052
                     /locus_tag="ACG8MG_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0375043.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33192..34115)
                     /locus_tag="ACG8MG_000978"
     CDS             complement(33192..34115)
                     /locus_tag="ACG8MG_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0375044.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34130..34402)
                     /locus_tag="ACG8MG_000979"
     CDS             complement(34130..34402)
                     /locus_tag="ACG8MG_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375045.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10186 a         7197 c         5950 g        11199 t
ORIGIN      
        1 ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga
       61 ggttgtaaaa tcagcggttt gacctgagta ttagagatac aaaacggaaa aatagcttct
      121 catattttgc cgttttttga gctaataccg aaggacagct gattgaacac cgtttattcg
      181 gaaaaaggga ctgtgacacg acttttgtca cagcccctaa aaaaaataat aaatttgtac
      241 tattctatga agcttcttct tcagaaaact gactgttata aagatctgca tagaatccgt
      301 ctttttccat caatgtatcg tggtttcctg tttctacgat cgaaccatga ttcatcacaa
      361 taatgttgtc tgcatcgcgt attgtagaca agcggtgagc aacaacgaaa cttgtccggt
      421 tttccaatag gcggttcatc gctttttgaa tcagtatttc tgttcgtgta tctacactag
      481 atgttgcttc atcaaggatc aggacatctg gatttgctaa gaatgcccgc gcaattgtga
      541 ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc gtttaataca gtgttatatc
      601 cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc tttagcagcc tcaatgattt
      661 gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc atagatactt cctgtaaaca
      721 gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa ttcatcacgt gaaagatcac
      781 gggtatctac accatcgtaa cgaatactac ctccagaaac atcgtagaac cgttcaagaa
      841 ggttgatcag tgtcgtcttc cctgcaccag tcggacctac aatagcgacc atctcaccag
      901 gtttgacatc caagttaaaa tcagtcatta gcaagttatc tggtgtgtag ccgaattgaa
      961 cattttcaaa acgaactttg tatggtgtat ctttttcttc tggaaggcca gatttttcat
     1021 tggacatctc ttcttcatcc aacacttcaa atacccgttc ggctgaagca actgttgatt
     1081 ggatagtgtt catcaaactt gcaagttgag tgattggttg agagaactgt gttgtatatt
     1141 gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt tgcaactttt actccgccta
     1201 gtacagcgac gaacacatag cctagatttt taacgaaatt catcaacggc atgatgattg
     1261 ccgaaataaa ttgagctttc caaccagcgg catacagatt ctgattttct ttttcaaata
     1321 cttcctggtc tttttcagta tgattgaatg ttttaacaat ggtatgacca ccatatgttt
     1381 cttctacttg gttattcaat aaacctaaac ttttttgctg atctgcgaaa aacacttgtg
     1441 atttcggcgc tacgatcatc acgatgatca agcttaaagg gatcatcaat aacgcgacta
     1501 acgtcaattg ccagctgatt gaaagcatca tcacgatgac cccaatcaat gtgactgcac
     1561 tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc gatgttatcc atatcattga
     1621 ttgctcgaga catgatatcc ccatttgtat gtgtatcata ataggaaatc ggtacacgat
     1681 tcattttggc ttccaaatca tgacgtaatt cataaactgt tcgttgggaa acacgtgtca
     1741 taataaattg ctgtaaaaag ttgaatacag cagaaatcaa atacatcacg attactgtcg
     1801 caatgatctg cgcgatttta tcaaagtcta tcggaaattt tgttacttgt tggccttgtt
     1861 gcatctgttg agcgccagcc attacacctt tgaatatttc tgtggttgct ttccccaaga
     1921 cctttggtgt ttggatttgg aagatcacgg ctgctattgc tagtacaaat acagcgatga
     1981 tcgcaaccat acgtttagac atatagccaa acaatcggcg taccgttccc cagaaatttt
     2041 taggcttttc accttttaca ccgagatttc tgccaggtcc accatgtcct ggaccgcctg
     2101 ctggtccatg agagggacgt gtgtttgttt tactcatgct aagtcctcct ctcttagttg
     2161 tgaatgaacg atttcttgat aagtttcgtt tgttgctaat agttcttcat gtgttccttt
     2221 gccaaccagt tttccttcat ctagcacaag gatcatatct gcatccatta ctgtacttac
     2281 tcgttgagca actaaaacgg tgatactttc tttcatattc gttttcaatg cttggcgaag
     2341 attggcatcc gttttgaaat ccaacgctga gaaggaatca tcaaacacat agatatctgc
     2401 tgttttgact aatgcccgtg caattgccag acgctgtctt tgtccaccag agaagtttcc
     2461 tcctccttgt tccacatggc tatctaagcc atcttccatc tctgaaacaa aatctttagc
     2521 ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt gcatccggct taccatactg
     2581 catattgtct cggatcgttc cagagaacag aactgctttt tgaggaacaa agcccattaa
     2641 ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt ccatttattt ggatcgaacc
     2701 agattcgatg tcatataatc gagggatcaa attgactaag gtcgttttac ctgaaccagt
     2761 cccccctatg attgcgacga tctcgcccga cttggcttgg aaatcaatat cttccaatgc
     2821 aagtttttct gctccatgat atcgataatt gacatgattg aaggccaatg ttgcgttttt
     2881 tccagcaaaa gaaactgttt taggattttc agggtcatgg ataccgattt tttcatccaa
     2941 cacttcatta atacggtcag ctgaagcttg tgcacgaggc accatgataa aaatagcgga
     3001 aagcatcata aagctgatca agatctgcat cgcataagtc ataaatgcaa tcagattacc
     3061 gacttccaaa gtcatatctg ctatgtagtg tccaccaaac caagtgattg ctacgtttgt
     3121 accgctcatg atcaatgtca tcattggcat catcaatgcg acgatcgtat taactttgat
     3181 tgccgtattt gtataatctt tattcgctaa atcaaaacga ttttcttcaa agcgtgtctt
     3241 atcgaatgct cgaatcactc tgacccctgt caatccttct cgaaatacca ggtttaaacg
     3301 gtcagttttc ttttgcatac ttttgaataa tggaacagca aagtacatga ttccgccgat
     3361 caaaataatc attactggga tgacaactaa gaagattttt gtcaactgat gatctttttg
     3421 ataagcaagt atgctcgcgc caatcaacgt gattggtgca ttgatcatca tacgtaaaaa
     3481 catttgtgta accatttgga tttgagtcac atcattggtc gttcttgtga tcaaagaggc
     3541 cgttccatat ttatcaaacg catcttttgt cagactctct gattttttat agacatccgt
     3601 acgcaattgc ttacccagtt tctgtgattc acgagtagca aaaaatgtat ttgcgatagc
     3661 agctaagata ctgatcaaag aaaagccgat catcacaaaa cctgtatgcc aaatataatc
     3721 gacatctcct tgcgctaccc ctttatcaat aatattggag gtaagtgtcg gcaaatataa
     3781 atctgccagt acttggatca ccatgaaaac tgcagctgca attgctgaag tcaaggagat
     3841 ccgttttacg atttttagca tctagttccc ttctttctat ttatatactc cgtttttcag
     3901 ccaagaaagt ttcaaattga attcactgat tgcttgctct actgagtaat cttcagatgt
     3961 tgaaaataag cgatacgaat gcgcaattgt tgtaaataca gtattcatca ctaattggat
     4021 cagtaattcc aattcatgat cattttttac tttcagcttc gtccgatcga tcaattcaag
     4081 caactctttt gcatcttgtt tgtgttcttt atgtggatcg tgaggcgttt tttggcgact
     4141 cgtatgagga gcgacttttc ttgaggcacg gtaatccatg ttcataaaca agttacgata
     4201 aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt ttagaaaaat aggattcgaa
     4261 tcctttgaat agatcaccat cggattcttt cattagttgt aacatatctt tagagctgtc
     4321 acgtctcatt gtctcaaagt aataaaaata aagatcctct ttatcttcaa aatactgata
     4381 gaaactgcct ctagggatat ccgctaattt gacgatatta gcaatcgaag cttcttttaa
     4441 gggtactcta gaaaactcga ttctcgcagc ctcgattagt cttttttgtt tttctttagg
     4501 caaatggaaa aatgtctgtg tcggcatatg gatcctcctt tctttcaaga tgacacattg
     4561 tcatatgacg ctgtgtcatt ataacacctc ctacctgttt tgcaagacat ttgcatagtt
     4621 tttttcatta tcatccgata cattaaaaaa gtgataagag gaatccttcc atttgaatta
     4681 atgagagttg cccattaaaa tagccacata ctaaacaaag gatgagataa atggcccaat
     4741 caattagtgt ccaagacttc aataaattgc ctctcgatgc tgacagcatc gttttagata
     4801 caagaaatac cttagattac acaaaaaacc acttaagcca ctcgctttct atccccgctg
     4861 ctatcttgcc aagacaatta aaaaatctag atccaaagaa actttattat attctaagct
     4921 atacaggcag gcgatcagaa gcattagccg atcaacttac tgctaatggt tttcgtgctg
     4981 tttatatcat tggtgggatg gaagctcttc agtcgagtgt tgcttgaata ttgcttgtta
     5041 gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct cttttatacc ctgtcttttt
     5101 ttagttattt attcttcaac cttatcaata tgacttgata gccttttctt tttctcatag
     5161 ttataacaat catagcaata aaaggagaag gtaagcatat ggcatctgtt ttagtagtca
     5221 aaggtcatcc cctaactgca gaagaatcgc gcacagttaa ggcattgacg tcttttttaa
     5281 caagttataa agaaaatcat ccagatgatg aagtgactgt tttggaactt tatcgcgatg
     5341 atattccgga aatcgatgaa gaattattat caggttggga agcattacgt gctggtgcag
     5401 aattcacttc cttatcagaa agtcaacaac aaaaaattgc tcgtttcaat gaattgactg
     5461 atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc cttatggaat ctaaatatcc
     5521 caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc tggaaaaacg ttccgctata
     5581 cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc actgcacatc caatctaatg
     5641 gcggtgtata caacggacaa gactttgcct ctcaatatgt aaaaggtatt ttaaactttg
     5701 taggcatcga tcaagtagat cagctattca tcgaaggaat cgattatgac cctgaccgtg
     5761 cagatgagtt gatgcaaaat gcattagata aagctgctgc tttaggtaaa tcattttaaa
     5821 aattgagata aatgcataca aaagagacag aggaaaaaat cttcaaagca gcatttgtac
     5881 cttggatatt ttcccatctg tctctttttg ttattctttc tgctttttca acggattttc
     5941 ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca tgagtaatca ctactttagc
     6001 tgctgcataa acaggaatcc ccaagatcac accgatgatt ccgaacaatt tcccagaagt
     6061 cagcagaact aataaaatcg ttactggatg gattgctaac tgattcccta gtactaatgg
     6121 agagatcaag cgtccttcta ttgtctgctc tactgcaaaa acgatcagta ctttgatgat
     6181 catgaatgga cctgctacaa tggctaaaaa gatagccgga atcatcgcta aaaatgatcc
     6241 taagtaaggg atcaggttca aaaaacccgc aataatcccc aacgtgacag catactctaa
     6301 accaatgacc gcaaaaccaa tcataaacat gattgccaca gcaaaagcca cagttagttg
     6361 tcctcttata taagaagaaa cttgactatt catttcactc agcacattca acgtcggttt
     6421 ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct aattgtttac cgtctttcaa
     6481 tagataaaat aagataaacg gcatcgtcag cagagccact aaaatagtcg ctaccgcgcc
     6541 gacaaaactt ccaagactct gaactgtact cttagatata tcttggatca tatcgcctgc
     6601 cgaattcatg aacttatcac ctgctgtttc caactgttcg cgaaattggt tgaacagcgg
     6661 gtcccgcaaa atctcggtta atttattatc aatcgtatct acatattgag ggaagttact
     6721 gatgaagctc actgtttgtt cctgaatctt agggatgatc actacgctcc cccatgcgat
     6781 tagtgcaaca accaaaacaa acagcccaat gatactgtaa agccttggta cttttcgctt
     6841 ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag attcctgcta aaatgatagg
     6901 cagacctacg attgctaaaa attgccagat tggtgcaaac aaatatgaaa cttttgtaaa
     6961 taaaaagacg atcagcaata ttaacaatac aacaagtaag gctgtgacta cttgattatt
     7021 taaaaaccag cgccaaaacc acgataccgg tctgttcttc ttattttttc cttccataat
     7081 tcctcctgtt agttataaaa aatttggctt cccaaaccaa tagcaggcag tctatgcgga
     7141 tgaagataga ttccatttgc aatttgtcct tcttctggac aagcatcaaa aatcaatgat
     7201 acgtgagcta ctgtctctaa attcccattg gccatcggac cgacataatc gatcgtatat
     7261 acctgatcat caaaagcaat tgttccgcct gctttcaaag aaaaaggttc gtcttttgat
     7321 acttcttgaa tcaccgaata ttttcgtaag gcagctgttg ctgttttatc gaacaaaatc
     7381 agcatgggtt cttccttgtt tattgcttct gtcccaattt ctgtaatctt tgcctgaatc
     7441 attgcactct ctcctttaat tgctttcaaa aacagtataa cacattctca ttctttagcc
     7501 gtgtggaata attatgtgat agaataaaaa cgaaaactat ttcattacaa ttaggaggaa
     7561 tcagctcata tgatcgaaaa gataatttta ggaacctata cgcgtcgcga aagtaaagga
     7621 atctatacga tcgaccttga tacagaaaaa gaagaattag ccaacctttc attagctgtt
     7681 gaagaaaata gccctaccta tgtagcaaaa agtaaagctg gcaatcttta cacagttact
     7741 agtgtggatg gattaggtgg tgccggtgcg tacgataagg attttcattt tttgaatgcg
     7801 gtaacagagt ctggcgctcc tttgtgttat gtagcggttg atgaagcacg tcaattacta
     7861 tacggggcga actatcataa aggtgaagta aatgtctacc atatattagg agatggcgaa
     7921 ctgaaagcag cagattcaat attccatcaa gaagcgacag gtcctcataa aaaccaagat
     7981 catgcgcatg tccactatac ggatctgact cctgaccaac gattagtcgt atgtgatctt
     8041 ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg gcaaattgaa tcttgtaact
     8101 accttcactg cagagccagg aacaggccct cgccatcttg tctttcatcc aaatggacaa
     8161 ttcgcttatt tatttggtga attagacagc acagtccgcg tgctttctta taataaagaa
     8221 acaggtgctt ttgaagaatt acagaaaata tcgaccttgc cagaagaatt cgacggtgaa
     8281 aatggagggg cagctatccg catttcaaat gatggcaagt tcctttatgc ttctaaccga
     8341 ggacataact cgattgctgt attcgcagtt tttgaagatg gttcccacat tcaaaacaag
     8401 caaatcatct caacagaagg ggattttccc cgcgatttcg cactagatcc aagcaataat
     8461 tttgtcgtgt gtgccaatca gaacaccgat aatctgacat tatatcgccg tcatcctgaa
     8521 acaggattac tggaattact gaaaaaagat gtctatgtac cagaatgtgt ctgtgtttat
     8581 tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc aagacgtatt agacagacta
     8641 cgggaagaac taaatattcc tttctttgat gggaaaatcg aagataaaga gtatagcgaa
     8701 gaggaatatc agaaactcaa agccgattta cagaactact ttgaacaata tgtacagaat
     8761 gtagaaaact aatcagcttt ccatactatc tgaaaagaac atcaggacaa gacttttccg
     8821 tctatcctga tgttcttttc tacaaataaa aaaccgccga gatggttaga ctcgacggaa
     8881 aaggagtatt tactcgattt tgagtaagta caaggttgtt gttggttata ggtatgtcat
     8941 tatcataaag gattttgatc actctatcat aaaggtaata ttacatttat attagataac
     9001 tctagttttt attgttatca aagatttctt ttattttttc aacaacaaat tagtcatttt
     9061 ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt tttgcttcag atgtattgaa
     9121 tagatcttca tctacccctg attcaagatc gatactttgg atcatttctt cacagcctgc
     9181 tacatatgaa agataagctt tttcaaaatt tttatgaatg cccattgctt ttgctggtgg
     9241 acgcaaagta ctgactttct taagcattgc ccgatatttt tttgttcctt cttcaaataa
     9301 caggatgatc tcttcaagag tcgatttgtc cagttcactt gtttttcctt catcaattgc
     9361 gtttctgatc acttcatatt tcggatgcat tccttctccg attgcttctg tttccttgac
     9421 gatttgatcg atggactgca cataatagcc aatattcggt aacatgttcg ttttcctact
     9481 ttctccattg ttgttattat ttattctact tgtttttatc ctcaaatgac aagtacttga
     9541 aaaaatttaa tggtttctat acagctcgcc attcttattc gtgctcaaag aaaccatctg
     9601 ctaaactata ttgtttccct gcgacaatgt catattggat caattgatat tcgtcgtaaa
     9661 tctcttgttg cgagcgattg aattgcgctt ctttatacca ctgtccgttt tgatagtaca
     9721 gttctcgttc aaacgccgga agttcctgtt ctaatgataa aagcagctga taaaagccat
     9781 ttgttgtctg attcgtctgt tccattagat tcgctgcaaa ataaaacgga ctggtgattg
     9841 catcgtgagt cgtttgcttt tctaattctc ctttactgtc gaacataaga aattgtgttt
     9901 catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc tgaatagatc cctggaagat
     9961 gatcccccca aaaaacaact aaagtccttc tagaaagatt cttcaattct tccgtgaact
    10021 gcttcagtgc cgtgctgcta taggaaatat cttgcagata attctctaag tcaagtgtcc
    10081 cacttccatc tgaagttttt gcaggatagt ctagattatc atactttcca tcgtagggca
    10141 tatgtgtctg catcgtgact aaatgaatga actgaggtgt tttttcttcc ttgagaagtg
    10201 tcattacttc tttgtaagct gactcatcag aaatatatgg attattctct atcgtatccg
    10261 tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc taatgtctga taaacatcct
    10321 ctcttttata catcgaagta ttgtatggat ggatcgctgt cgtctggtat gattgtgcgt
    10381 ttaacgctga aacgatagaa ggaagctgat ccatttttgg tacaagcatc gtgtaagggg
    10441 tggtgagttg cgcattaaat aacgccatag aaaaaccagt caatgcttca aattcgatat
    10501 ttgctgttcc accgccatag ttttgagaaa gcatattgcc actatatgtt tgatcagcta
    10561 cttcataata atctgccaat ggctcaccag atacttctac gccgtttaat cgactaggat
    10621 ctgagaaact ttcactcatc acgaacacga tgttcggcga ctcttcctct acagttttat
    10681 ttttttcatc ggctagcttc tggtattttt cagtgatttc tttgatcttt tctttcgaat
    10741 atccttcagg ttcatccatt ggttctacct tcagattgta caaaaaccca ccgataaaac
    10801 cagtgttata gtagttcatt ttctggctgt aaggtatcca tagtgccgtt ttattatatg
    10861 cttttcgcaa caaattatcg ggattgttga aattactgat ataccctagc agtccaattg
    10921 ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct tccttttttc aggcttcgaa
    10981 acatgtacca gcaaaacaat cctatcacgg ttcctgcggc agctaagatc actgtaaaca
    11041 gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt gatcatcttc aaatcgtctg
    11101 gataaatcgg ttcttgccga taactcattt tcatataatt tgcaaaacct aatacaccga
    11161 tggctacgac atacgtaagg gaacctaaag ggacagatcc tgctagtgca atcagaaaaa
    11221 taagaataat cagcaagacg agacaggcta agaagaattt ttccgtatgc caagaaaaag
    11281 caaatttcaa cgcaaggtct acggatagat cattttgaca ccactgcaaa aataaattac
    11341 taaaaacaac aagaaaaaag atactgatga ttgctgctgt tccttttaaa atctttttct
    11401 gtttggtcga aagtctgctt atatatcttt tcaaatgctt gctccttcat ttctatcaat
    11461 aaaagaatgt tgcttctctt attttagttg tacgaaattc gaaagtaaag aacggaaaga
    11521 agaattaatg aaatccaaat attctctttc tttatggcct ttctggtttc tttaataagc
    11581 ggacctttcg tactccagaa attttttcta gctgttgttt cacttgattg agctgttcaa
    11641 aatcagtact gtctatgtca atcaatatgt agacatgctc attcttatgg ttacgcgata
    11701 tatctgcgat cgctagctcg ttctcattca gcagagaaaa tatctctgct aatatattgg
    11761 ttttcttttg ataaaatata gtgaatcgaa acggcgcttg gaaaaacatt cgtgcggatg
    11821 gataattgac cgcttctcta accgtaccga acaaaagata atcttttaag gcttcaactg
    11881 ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc ccctagatga ggcaacataa
    11941 gtatacggtc attttctaag aactcttctt ctggaaaatc agtcaagtat ttagccagat
    12001 gttgtttttc caatgcttgc ataacagcat atttatcaac aatttcactt cttcctacat
    12061 tgataagtac agcatctttt ttcatttttt ctatattcgc ctgatcgatc aatccttttg
    12121 tatcttctgt caacggcagc atgactacga tgtaatccga ggtagaaaga actgtttcta
    12181 aatctgcttg ccgaacataa tccaactggg catcgcggat agaataaccc aagacatcca
    12241 tccctaagct atagcaactt agcgctactt ttgtgcctat cgctccaaga ccaagaagac
    12301 cgatggtttt gccttgcaat tctcgtccca cataagcact tcttttgttt tctgcctgtt
    12361 ctaaaatatt aggacctgtt agtgtttgta ccatacgact tgcttcgatt atcggacggg
    12421 aagataagag caaacagcac aaaacaagct cttttactgc attagcattt acacctggtg
    12481 tattcatgac gatcgtcccg ttttcagaag ctttttcaac gttgatcgtg ttcaccccaa
    12541 caccagctct tgaaattgct agcaaatctg gtgtaagcca ttcatctttc actttcgtac
    12601 tacgaataac tattccttcg gctggcaaat catctattat tttgaaatat tccccttgta
    12661 acttttctat ctcttgttct ggaatagcat caatcaaatg aacgtgatac ataaaacccc
    12721 ctccttacta acaagtatct cgaaatttct gcaattagac aactgtttac catttgactt
    12781 cctagaagca aaaagagcag ttagagcaga gtaggctgag tgataaaaat aaggctatga
    12841 caagttactg ccataagcct tattttcgag agcaaaatat tgagggtgag atatcttgat
    12901 aaatggtgtt ccgttagctg acattgataa taagactaaa tttcaaaaaa gttgaaaagc
    12961 aaatttcgtc aatctcttct tatttcttaa aactaagtgg ctttttcaca acctcttttt
    13021 atctaataac cactgtttcc aactcaacta attttgccca cgtttcaatt tgatctgttg
    13081 ttagatttag cgagacaacg gtatgatgac cgccacctgc ctgaatccat gaatggacac
    13141 cttcatggaa attcggttta gttttccata acacacgtgc aacaggtaaa ttaggtgctt
    13201 cagtcgttgg ttcaaaggct tctacttcat tgatcagcag tttataatgc gtaccgaaat
    13261 ctgccatcga tacgactacc ccttctcctg ctttcccatc aaatacaaga cgcgccggat
    13321 cttctctatc tcccattgat aaaggagaaa caacgattct tggtttcgtc gcagcaagcg
    13381 tcggatctac ttccatcata tgggattcca agattgcttc ttggccacta gctagctcgt
    13441 aagtataatc ttccatgaat cctgtatctt tccctcgagc catgattttc agcaaccgat
    13501 caatcgctgc agttttccag tcgccttccc cagcaaaacc gtatccttct gccatcaaac
    13561 gttgtacagc tagtccaggt agttgtttca ttccatacag gtcttcaaaa ttagtcgtaa
    13621 aagcgttata gcctccttct tctaagaaac ggcgaatacc gatttcctgc tgagcttgta
    13681 cttttacatg ttcctcccat gtcttttctt cataatcacc atagtcgaag gtatacaatt
    13741 ccttgtactc ttcaaataat tgatcgactt cttcgtctga tacgcgatcg attacttgta
    13801 caagatctcc gataccaaaa taatcaacag tccaaccaaa ttgaatctgt gcttccactt
    13861 tgtctccttc tgtgacaccc acgtttctca tgttgtcacc aaaacgagcc acttttattc
    13921 caaaactttc attataggct actgcaacat ccatccaatc agcaatctct ttttggatct
    13981 caggtcgttt ccagtagcca acgacgattt tattttgttt atttaatcga gcattgatga
    14041 acccatattc gcggtcacca tgagcggatt gatttaaatt catgaaatcc atatcgatcg
    14101 ttttccaagg aatgctttcg ttatattgcg tagctaaatg aagcaatggt ttttgtagaa
    14161 gctttgtccc gcggatccac atttttgctg gtgagaaagt atgcatccaa gtgatcacac
    14221 ctgctacttc ttctcgataa ttgacctctt tcatgatttt tgtgatcgta tcagcagtaa
    14281 ctgctaattc ttgaaggcgg attggataag gcaattggcc attttcattc agttcatcga
    14341 ccatttcttg tgcatgtttc tttacttctc gcagtgcttc ttccccatat aaatgctgtg
    14401 atcctacaac aaaccaaaat tctttttctc caatattcaa cataattagc cactcctttt
    14461 atttgttttg tccgtaatac gcattttttc cgtgttttcg taaataatgt ttatctaaaa
    14521 tccgttgagg cagctcttct gaaaatgaat tgagctgtct ggtaaataaa ttcatcttgc
    14581 atacttcatc tagtacgact gcgttcatta ccgcactttg cgcatctttc ccccaagtaa
    14641 atggaccgtg tccgtgtaaa agtacccctg gaattgctaa gggatcaatt ttgcgttctt
    14701 tgaatgtttc tatgataact tttcctgttt catattcgta cccgctgtcg atttcctgtt
    14761 gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt atctgcatgc gtcgtcccca
    14821 tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc ccatgtcgag tgagtatgga
    14881 cgattcctcc gatttgagga aatgaacgat ataaaacagc atgagtaggc gtatccgaag
    14941 agggattgag tttaccttca atgatatgat tgtccaagtc cacaacgacc atgtcgtctg
    15001 ctgtcatctc ttcataactg actccgctag gtttgataac gaaatatcgt gtttcaggat
    15061 caaaagcgct cacatttccc catgtatatt tgacgagtcc gtgtttaggc aagtcaagat
    15121 ttgcttggta tacttcttct ttcagttgtt ccagcatttt ctatcactcc tttttcttgc
    15181 aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac ggtaacgtct cataaatatc
    15241 tgatagcctg ctatttcttc tgctgaaggt gtgatggtga ttccttggtc ttgtccaaac
    15301 acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc gatccatcat ataggctgct
    15361 aataacgcga ttccccacgc tccaccttct ccggctgtgt ccatcactgt aactggtgcc
    15421 tccatagcag atgctagaat ccgctgtcca acttctggtg ttttaaaaat ccccccatgc
    15481 cccactaaac gatcaatttg gacattctca gatttcaata tatccattcc gattctcatt
    15541 gcaccaaaag cactggctaa atggatccgc atgaaattcg ctaaatcaaa acggctacct
    15601 ggtgtacgta caaataatgg ccttccttca tttactcctg taatgttttc accggaaaaa
    15661 taaccataag aaagtaatcc cccgcaatcc gtatcgcctt ctagcgcttt gttgaataat
    15721 accgtgaata atttatctct gcttatctcc atacccaaag aattggtaaa ttcttcaaaa
    15781 atactgaccc aagcattgat atccgaagag cagttattcg tatgaaccat gcctactagt
    15841 tctcctgaag gtgtagtgac catgtcgatt tccggatgga cattttctag ttctttttct
    15901 aatacgatca tagcgaaggc ggaagtacca gccgaaatgt tccctgtccg tttggctaca
    15961 ctgttcgtag cgaccatacc ggttcctgca tccccttctg gcggacaaac cgggattcct
    16021 ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg attcgcttaa cttgccagct
    16081 tcttctcctg ctactagaac tttaggcaaa atgtgatcca agtcaatcgc tacgtgcgct
    16141 ttttcgatta actgaccaaa aatatttttc atttcttgat ggtaggtttt cgttgaagga
    16201 tcgataggaa acatgcctga tgcatctcct acgcctagta ctttctcacc agtaagctgc
    16261 caatggacat aacctgcaag cgttgtcaaa aaattaattt ctttcacatg ttcttcttta
    16321 ttcaaaatag attgatacaa atgagcgata ctccatctct gcggaatatt gtattgaaat
    16381 ttttccgtta atattcgttc tgcttcttca gtgattgtat ttcgccaagt acgaaatggt
    16441 actagtaact gttccttgct atcaaatgct agataaccgt gcatcattgc actgaaccca
    16501 atcgacccga ttgtcgtaag attctcgcca tagatttcga atacttctgc tgccattttt
    16561 cggtaactga cgcgcagtcc tttccaaata tcttctaatg aataggtcca gataccattt
    16621 tctagtttgt tttcccactc ataactacca gcggcaatag gagtaaaaga tgaatcaatc
    16681 aatacagctt tgatcctagt tgatccaaac tcaatcccta aagatgtacg accatctatg
    16741 acatctaaca tactttgcgc atttacttcc ttcataatga tttaactccc tccttctcgt
    16801 caaacctgat caaaccgtct ttttagtagc gcttacaatg attattgtat acttttgtac
    16861 gtacatgtca agcggttatt tacaagaata caataaaaaa cacttgtttt gtacgtacat
    16921 aagttataaa atgaaattgc ttacaaaatc tgatcaaccg ccttctcgaa attctagaag
    16981 gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat cgtttattta tttttttggc
    17041 gcatttggcg gcattctatt tggatatgat attggtgtta tgaccggcgc cttacctttt
    17101 ttacagcatg attggggttt ggcaggtaaa gcttctttga taggatggat cacgtcttcg
    17161 gtcatgttag gtgcgatcct tggcggctct ttatctggtc ttctttctga taagcttgga
    17221 cgacgaaaaa tgattcttct ttctgcactt atttttatgg ctggttctgt cctttctgca
    17281 tctgcccctc acaatggttc ttattttctg attgcagcaa ggattcttct aggtttagct
    17341 gttggcgctg catctgccct agttcctgct tatatgtctg aaatggctcc tgctagacta
    17401 aggggaagat tgtcagggat caatcaagtg atgattgctt ctggcatgct cctttcctat
    17461 gttgcagatt atttgctaaa agtgttgcct gaaacgatgg cttggcgcgt gatgttaggg
    17521 ctcgctgctg tccccgcgct gattttattt ttcggtgttt tagctctacc agagtcaccg
    17581 cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa gagtattgaa ctatattcgg
    17641 acaccaaaag aagctgaaca ggaattcgaa caaatccagc tgaatgtcaa acaagaaaag
    17701 acaaccggaa cttcttggca tactttgttt ttagaaaaat atcgttcctt agtcattgcc
    17761 ggtattggtg tagcagtatt ccagcaattt caaggagcta atgctatttt ttactatatt
    17821 ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg atgcattgat gtggccgatc
    17881 atccaaggaa tcattttagt tgctggttct ttgctctttt tggttattgc agacaagttc
    17941 aatcggcgaa ctttattgaa aataggcggt agcgtgatgg gactttcatt catcctacca
    18001 gctgttttag gaactgtact ggatgctcat acaaattcgt tgttgattct cttgttttta
    18061 tgtatttatg tcgcatttta ttcttgcaca tgggctccac tgacatgggt aatcataggc
    18121 gaaatattcc cactagctgt ccgcgggcga gcatctggcc tagcctcttc ttttaattgg
    18181 attggttctt tcctagttgg cttgcttttt cctgtgatga ctgcttctat gtctcaagaa
    18241 atcgttttcg gcatttttgg gatcatctgc tttttagggg ttttatttat tcaagagatc
    18301 gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac aaagtgcatc taaaaaaact
    18361 tatcctaaac gtatttctat ccatcattct taaagaggtt gtgacagaag tggtcagctt
    18421 caagaaataa gaaggaaccg cttagtttcc agtcacaagg aacaatttca aaaaacagct
    18481 tctcatgttt gatgaatatt gtgacttgtg accagttggt aggagaacac cgtttagtgg
    18541 agaggtggtg aaagaaacgt tcagccttga gcgatgacaa aagctctcac aaacattgcg
    18601 acgagtaacc gtaggagcaa taagaggcgg taaaaacccg tctcttattt ttcgttttct
    18661 ttcactgttt cccgttcaac tagtacaggt tcatagagaa tatttggctg tgcctgtcct
    18721 ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc ccaacttttc ttttggatgc
    18781 gtcaatgttg tcaatttttt attccctaaa tggcttaaag aagaatcgtc tgttcctacg
    18841 atcgataggt catctggaat atgatagcca ttttctgtca acttatctag cattaaacta
    18901 gctatttgat cgttatagca aatgatgcca tctaaagctt gttcttcttt caaccgttgt
    18961 actgtttttt cagcgattgt ttctcgtgtt tccgtggtga aagtaatgat atcttccgtc
    19021 aaaaaacgca cattatactg ttcacacgcg ctgataaaac ctttcatcct gtattttcct
    19081 tgaagatcgt cgatttttgt gatgaacaac agacgctgat gtcctttttt tatcaaatat
    19141 tctgctgcca taaaaccgac tttcacatca tctaaacaaa tataagaggc atttaactct
    19201 tcataagcag cattgatcat caccatcgga atgccttgtt ctctcaaagc aacataaagt
    19261 gctaagtttg ggttatactg attacttttc gtaggctcaa cgatcaaacc atctacccca
    19321 taagcaatca ttttcttcaa acactctttc tccaattcgt gatcgttatt cgtactggct
    19381 aaaagcaatg aatacccaga ttctcgtaat tcattttcga ttcctcggat aatcgacgga
    19441 aaaatataat cagagagata tgtggtgatg acaccgatcg tttttgttct gttcaatggt
    19501 gttttttctt tcttatagct gtcatctaca tatgtgcccg accctttttc tttacgcaga
    19561 tagccctcat ttaccaacac agcgattgct tgccgaatcg tatgtctgct gacgtcatat
    19621 atttcttgca gcttgaattc tgagggaatg gttgtattta cttcgtattc ctcacgaagg
    19681 atcttttgtt ttatctcttc tgcaatatct tgatattttg ccatttgcct tgcccctttt
    19741 tatttgtacg tacatatatc ataacgagtc tgcaaggttt attcaagcaa aaatcaaggt
    19801 ttcttgtgct cataaggcat acggaacagg ttcaccaaat tctggaaatc cttgctcatt
    19861 ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg tctaacggat cccctttttg
    19921 gttcttctct ggtcgagcat gatagatcaa aatatcaaag cacccgtctt ctgaaaccgt
    19981 aaacgagttg tgaccaggac caaattgctt atttttttca gaagaaacga atactggctc
    20041 cgataattta tgccatgatt cttttaataa aagattcttg tgctcatcag cccacagcag
    20101 gcccatggca tagttttcat ctgttgcact accagaatac gtaatgaata ttttcccatt
    20161 gcgagtaagg actgctggac cttcatttac tgaaaaaccc cgtttttccc attcatactc
    20221 aggtatagaa aggaggatct gcttcccctt taacgtccaa ggattatcca tttcggatag
    20281 ataaagatta gaattcccag taatttcagg atcttgttgg gcccacacat aatatagctt
    20341 tccttgagaa gagaaagttg ttccgtctaa actgaaagtt tcccttttcg tttttatttg
    20401 tcccttttct atccaatctg tattcattgg atctatatgc tgattttcaa gtacgaacat
    20461 gcggtgatga tgagattcat cacgtatagt tttatgattg cttgcagcaa agtaaatata
    20521 ccattttcca ttgatataat ggacttcagg tgcccagatc aattcactca tcggtccttc
    20581 ttcatgagcc tgccatacaa tcaggcttgc agcatgttct aattcattca gtgactttgc
    20641 ccttcttaat tcaatcactt ggtagcctgg aaccgatcct gtaaaataat aataaccatc
    20701 tgtatgttta tatacccaag gatctgcccg ttgccgaacg ataggattat tataaagtgt
    20761 cttcatcata acaaatttcc tcacattttc tttattctaa ttacattcca agagtatttt
    20821 tctatcccta catttaattc gtcatgtttt tctattgaaa aatagtttat ttcaggtttt
    20881 attttttctg gttcttcacg tgtatttttg tccaacagat cgctactggt taatgtgtag
    20941 tgctctcctt gatctagcaa attgaaatca tgttcaatcg ttatcttttg ttgttcattt
    21001 gtgcgattga tcaagaaaat agtcagctga ttgccgttat ctacgatcac actgtccaca
    21061 taaggaacat tttttatctt ttcacaatca taaacagggc aaatgatttg ttgtttcaat
    21121 acttgacctt tagcatattt cgataagtgt tgcatcacat aataagttgg attcctccat
    21181 gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt ttactaacaa tgattgacaa
    21241 ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc ctaatgaacc aaataataat
    21301 gtatcttcca tagtaaagta acaccaatcg atgggactac cgacttcaaa atccctatgt
    21361 cttttttccg gttgggcatg tacattccat tcatcaaacg ctagatacat tgttttctca
    21421 cttctcaaat atccttttac ataatcacaa gtagcaacga tttcttcaat ttgacgatcg
    21481 aatcgataag cttttgcaag gtatgtcgct gtatcatcac gctcattttt aggaggttta
    21541 gtcaagtctt cttcatcata cttatcaata tagttatgca aagctaaata atctacattc
    21601 tcataagctt cttctaatgc gatacgatcc cattcagggt acgatggtaa acgtggcgta
    21661 gagcttccaa ctaagattgt ttcaattgaa tcatccacta acttcatgac ttttgaggct
    21721 tcatttgcta acctaccata ttcataagca ctcttagccc ccacttgcca tggaccgtct
    21781 agctcattcc ctaaacacca taatttaacg ccaaaaggct ttttttgccc attttttatt
    21841 cttaaatcac tccagaaggt tccttttgga aaattacaat attctactaa attttttgct
    21901 tcatcaattc cttttgttcc taaattcacc gtgaatatcg gtttcgcttg tacatgactg
    21961 atccatttaa aaaattcgtg gagtccaaaa gaatttggtt ctatacctcg ccatgctaaa
    22021 tcaatttttt ttggtcgttg ttctttccta cctacagtat ccacccaatc ataaccagat
    22081 gtaaagtttc ctccaggata gcgaatgaca cctaattcta attcctttac ataagagagg
    22141 acatcttgtc taaacccgtc accatcagct gtttcatggc ccggttcata aatccctgta
    22201 tagacgacac ttcccatatg ctcaacaaat gagccaaaca gccgagaact gatctctcct
    22261 accgtaaaat cctctctgat ttttactttc acttcttcgt ccaacagctt tccctacctt
    22321 tcatttccta tagtcaagat aatagataca agtttccata cgccaccagc aaagataaaa
    22381 ataaaaggag ggaaaagaaa tatgagcagt acaaatccta ataacaatac gcaacaaaga
    22441 atatttctca aaaaatcaaa aaatactttc catgcacttt gttcaaaaat ttgtaaaaat
    22501 gtgatatctt tgttttcaga aatcacatat atccaggata aaagaaacat ggaataaata
    22561 agtaaaacta catatccact aattgaaagt acgattgtct gttcttctaa taattgatta
    22621 acaagaaaat aaataatgat cgttaacaga ctaaggaaca agttcttacc aaaatgttgc
    22681 tttattcctt gaaagaaaaa ataaatgata tttcctgatc cctcaccttt ccattgcgcc
    22741 attacataaa aaacagcaca tgtagcaggc acgatagtaa taacaggcaa acacacgatt
    22801 acccagagaa tacctaataa gatgtaatca gaaaccacaa tcaactgtcc ataaaattta
    22861 gtctcaacta ttttcatctt cctcactctt tcattccaga catagctgtt ccttgaacaa
    22921 aatatttctg tgcaaaaata taaaccacta gtaatggcag cattgaaata accgttccag
    22981 ccattacagg tccataaaaa gaaatgttct gccctgtaaa taaagctaac cctgctggta
    23041 aagttctcat ttttgaacta cttgtcaata tcaatggata aagcaagtca ttccaactat
    23101 tcatcagcgt gaaaatccct aatgataaaa gagtaggttt gctcaatggc aacatgattt
    23161 tcaaaaaaat attaaactca cttaatccat caatgcgtgc tgcctcttcc aaagaatctg
    23221 gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa tgcagttgtc atacgaggaa
    23281 tgatcaaacc tgcataagta tcaagcatgc caagcaaatt gacttcaata aatagaggaa
    23341 tcatgaagac ttggaatggg atcatcattg tcagcaaaac gaaatagaac aaaactgatt
    23401 ttcctttgaa acgcatacga gcaaatgcat accccgcaag agaatcaaag aatacagaag
    23461 tgatcactgt ccctccagca aaaatcactg tatttttgat gtaatctaat aaagggatcg
    23521 ttgtccaaac cttaatatag ttatctaggg tatagctatc tgcaaatatt ctaggcggaa
    23581 atgagataat gtctttttcg tgtttaaaag aagaaataat cagccacaaa aaaggaaacg
    23641 caataaccaa caatataact agcatactaa tcgttcgaat gattttttta ccactaactg
    23701 cttttcgact attcacagta actgtaatcg agctttctgg tgccatacag gtttccctcc
    23761 tatttcattt gtttttcttt ccgtgacatg tacacattag tggagatagc tatagttgca
    23821 acaatgataa acaaaaccga agacagtgct gatgcatagc ccaattcata aggtgctgta
    23881 aatcctcgac tgtaaatata ttgaacagct gtttctgtct taaattgtgg gccaccagcg
    23941 gttgcaacat atacttggtc aaacacttgc atcgaaccaa tcagattcgt taaaatacaa
    24001 aaacctaagg agggaacgat ctgaggtagc gtaatgttaa aaaactgctg tcgatttgtt
    24061 gcaccatcca tttgagcaga ttcatacaaa gagggagaga ttccctgaat agcagtcaag
    24121 agaatcgtca tagtaacacc aaaattcttc caaacagtca tgatggctac cgtcggcata
    24181 gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc cgacttgtga aaacaagtaa
    24241 ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa taatcgcaat tgacgtcaat
    24301 gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac gattgaatct ggtattttta
    24361 gataatgcta ctgctgctaa taaaccaacg ataatttgtg ttggcgtttc gagtacggtg
    24421 aaatataaag aatgaatcaa cgaattaact gctcgttcat cactaaatat acgtgtgaaa
    24481 ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat tcaaatctgt aaaactaata
    24541 gcaaatgttc ctaaaagcgg tatgacagta aataataata aaactaaaaa agatggagtt
    24601 aaaaacaaat acgcagcacg atcaggacga ttccaatatt tggtgaatcc gttcatatag
    24661 tccacctcat ctttcatttt gataaaatat acaatcttgg tttaaacgaa tctgaataag
    24721 atatccgaac aatcgctgtg atttttgcaa taacacctat ctgcaaatac taggtcgatc
    24781 attcagatat ctgtatcttg ccaacagtct atcctgtata aagttctgta ctatttttgc
    24841 agtaattgat tgatggcatc atctgcatcg ttcatgagtg tctcaggatc ttctcctgct
    24901 aataattttt caatcagtgg attgatgata tcgttattga tcgcagagat ttgggtaaaa
    24961 cctggtaaat atggctctgc atagtccatt tgtttcgata attcagatac aataggatca
    25021 ttttttactt cttcatcttt tgctactgaa tccaagtagg caggaaaacc attttcgata
    25081 ctccattttt taccaatttc agttgtattc caatatttca aaaactcata gacagcttct
    25141 tttttattat tatctgtact tgccggaatg gcatatccta cgccatctaa aatagctgtt
    25201 tttttgtctg aatcactccc ttgtggtaag gtagtcacat tataatcaat ctcgtttgct
    25261 ttcaatccgt tatttaacca tggcccattg atttcaatgg ctagttgacc agcattcatt
    25321 aaattgtcca tttcggcgcc agaaatcgat tctggaccag tcttgtctac atggatcatt
    25381 ttttggatcg cttttaacgt tttaaggttc tcaggagacg caaattcaga cttagtataa
    25441 tcatcgttca ctaatttacc gccatttgct aacatccagt tatataacgg tgcagttcca
    25501 tccttattga agacaaaacc tgatacattt ttactactat cagttaattt tggtgccatt
    25561 tctgtcaatt cttcccaagt agtaggtggt tgttcagtat ctaatccagc ttgtttaaac
    25621 aaatcattat tccaatacat atacatgcct tgtacctgca tggggacaaa atactgttta
    25681 ccttctactt cacctaattc aatagccgtt tctttaaaat cagatttatc tactccatca
    25741 tattcccaga aatcgtctaa tggttgtacc gctccgtttt ctacatactg tgcaaaatct
    25801 ggataattta atgcaataaa gtcaggtgct tttttagaag aaatcgctgt aggaagtttt
    25861 tcattcaaat tggcccaagt catgatatcc atttcaattt tgatgttttt atcatttgtt
    25921 ttattaaatt cattaacgat ttcttgcaat atttcaccat cagatgcagt aaacccattc
    25981 caaaaagtca aagtcacttc tttatcagaa tcagattctg ctgaacttcc gccaccgcaa
    26041 gctcctaaaa tacctgttgc agctaccaac aaagagccaa ttaaccattt tttacctttc
    26101 attttttgtt cctccttcat ctgataaacg acatctgacc acattccaag atagcggttt
    26161 caataaacaa ttgattttcc catcttcagc ttttacattt gttgaatgac ttggttttac
    26221 ttgttcactt ttaggaagat tagttgcttt tgtatcaaat ccactcattt cagaaaaatc
    26281 aatcactccc tctaatgata attcagacaa gtgaacagtt agattcattt cttcttctcc
    26341 acggttaact gcaaatatga caagttcttt gtcatttatt acggctatac tttctaaata
    26401 cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa cgaatttgtg ggcttaacac
    26461 ctctccttgt ccatacacag cagcttgcat aaatggataa tagatcgtct gtctccaagc
    26521 ttctccgttt gtttctgtcc tgattggtgc aataacgttt accaattgag caagacaagc
    26581 aatctttact ctatctgcat gtttcaataa agtgattaat agacagccta atagtaatgc
    26641 atcttcaaaa ttatagttat cttctagtaa aggaggagca atctgccaag gcgctgcttg
    26701 ctcatcttgt tccgctgaat gataccaaat attccattca tcaaaggaca ggttgatctg
    26761 cttcttacta tgtttcttcg ccttgactgt atcgcacatc gctgtcactt cagaaatgaa
    26821 ccgatccatg tctaaggaac gtgcaagata atgagaaaga tcattttttt ggttgccata
    26881 atattgatgc agagacaaat aatctatttg atcgtacgct tgatcaagaa ctgttaactc
    26941 ccaatcacca aatgtaggca tttgccgatt cgaacttccg cataaaacca cctcaattga
    27001 atcatctacg agtttcatcg ccttagccgt ttcctctgct aaccgtccat attcttccgc
    27061 tgttttatgt ccaatctgcc aaggaccgtc catttcattc cctaaacacc atgtcttaat
    27121 tccaaaaggg ctctcttgac cattcgaacg ccttaaatca ctccaataag ttcctttagg
    27181 aaaattacaa tattcgacta aattacgagc tgcatctact ccacgagtac ccagattgac
    27241 agccatattg atttcggccc ccacatgctg acaccagtta taaaattcat gaatcccaac
    27301 ttcattcgtt tcaattgtcc gccaagctaa atccaacctt ctaggtctat ttgctatggg
    27361 cccaatactg tcttcccaac gaaatcctga cacaaaattt ccaccaggat atctaatcat
    27421 gggaatgttc aattctttta ccaagtgaat gacgtcttgg cgaaatccac ttttattagc
    27481 tgatggatga tctggctgat aaatcccttc atatactgct cgtcccatat gttcaataaa
    27541 tgaactgtac aaccgcggat caactttgcc tacacgcttg acactactaa tcaacattgt
    27601 ggcttccata actgacctcc tttaatttat acatgaataa tacacctcgt tgaaagcgtt
    27661 gtcaataata taaatgttaa acttacttat tttttatcga ttttaattaa gcaaaatatt
    27721 aataatgatt aaaacaaata atttaatata acttttatta acaaatatgt taatatataa
    27781 gaaaaacctt ttaagaggtg atatatatgc aactacaaat aaacgaagaa tccttaccgg
    27841 tatatgaagc attagctagt aaaacaagga tcaagatcat ccagctactt tccaaaaaaa
    27901 agatgaatgt caaagatttg gcaaaagaac ttggagtgag cagtgctatc acgacgatgc
    27961 acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga aaaagtagga caacaaaaaa
    28021 tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc agaaaaaata ttcaatgctt
    28081 ttgatacgaa agaaacatcg atacctattg gtcactatac aaattatgca attgaaccaa
    28141 catgcggttt agctacgatc catgatttta tcgggaaagt ggatgaacca cgctatttta
    28201 tggatcctcg aaggatggat gcccgcattc tctggtttac cagcggattt gtcgagtatc
    28261 aggcacctaa ttttttaaat acagaggata cattggagat gttggaagtt tctgtggaaa
    28321 tttcttctga atttcctttt tcaaatgaca attggccttc agatatcact ttcagcctaa
    28381 atggtgtgga gctaggaaca tggacgagcc ctggagattt tgctgatatc cgcggtaaat
    28441 atacgcctga ttggtatcca gataatctta atcaatacgg cttgctgaag acgatccgaa
    28501 ttaccaaaca cttgacgaat atgaatggag aacccttgtc aaacatcacg atcaacgaca
    28561 tccctaaaga gcaagacact tggcatttgc gaatcgaagt gaaagacgat gccaaacatg
    28621 tggggggctg tactttattt ggtaaaggat tcggtaatta cgatcaggat atcaaattaa
    28681 aagtttacta cagctgatat ttttcttttc tattttataa aacaaaaaca gccaatccct
    28741 atgaagggac ggctgttttt gctttattgt tatttccaga aatcatccaa aacggaaatt
    28801 ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt cgattttttc ttgtgcttga
    28861 gcgggaactt cttttccttc tagataatca tcaatttcat tatatgtgac acctagtgcg
    28921 acttcatctg caatcattgg tttcccatct tcaagatctg cagtcggaat ttttgtgtat
    28981 aatttttccg gtgcattcaa tgcttgaagc aattgtttcc cttgtcgttt gtccaaacgg
    29041 aataatggca aaatgtcggc tccaccatca ccaaatttcg tgaagaatcc agtaatgttt
    29101 tctgcagcat gatctgtccc taatacggct cctgctcttt ctccagctac ggcatattgc
    29161 gtaatcatac gttgacgtgc tttgatattt cctttgttaa aatcgctgat tcccacacct
    29221 gcttcgctta atacttgtac ttgtgcatca acagctggtt tgatatttac tcgcagacta
    29281 acatcgggct ggataaagtc aagagcagct tttgcatctt cttcatctgc ttgttcacca
    29341 taaggaagac gaacagcgat aaattgatag ttttcatctt ttgtttcttc acgcatttct
    29401 tccatcgtta gttgcgccaa acggcctgct aaagtagaat cttggccgcc gctgatacct
    29461 agtacaaaag ttttaagaaa cggatatttt ttcaaatatg ctttcataaa gtcaatactt
    29521 ttacgtattt cttcttttgg atcaattgtt ggtttgacac ctaattcatt gataatttct
    29581 gtctgcaggc tcatttattc tcctcctatg cttctgtatg aagttctgct acgctttttt
    29641 tcattcgttc catgatagcc attttatggt tccagcattc agtagacaga tcgactgggt
    29701 atttttgcgg attcaagtct cgtttatact cttcccatag tgaatctaga ttttcttttg
    29761 tatattcttt gatttcgttt agtgcaggta attcgtaaac tctttttcct tcgataaaga
    29821 tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt tttattgata aacgtatgca
    29881 ctggatggaa catgaagata gcttcttctt ctcgagggtc ttcatcccat aatgtcacgt
    29941 agtctccttc agatttccca tcaaaatttc gagtgatgcg ccacacttgt tttttccctg
    30001 gagtagttac tttttctgca ttgcttgata acttgatcgt atccatcatt tttccttcat
    30061 catcttcgat cgatactagc ttgaatactg caccaagggc tggctgatca taggctgtga
    30121 tcaattttgt cccaacaccc catacatcaa ttttagcttt ttgcatcttt aaattcaaaa
    30181 tcgttgcttc atccaagtca ttcgaagcat agatttttgc ttccgtaaat ccggctgcat
    30241 ctaattgttc ccgaacacgc ttagatatat aggccatatc tccactgtcg atgcgtacgc
    30301 cttggaagtt gattttgtca cctaattctt tcgccacgcg aatcgcactt ggcacacctg
    30361 atttcaacgt atcataagtg tcgactaaga acacgcagtc tttatgggtt ttagcgtagg
    30421 ccataaatgc ttcataatca tttccataag attggactaa cgaatgagca tgggtcccgc
    30481 tagctggtat cccaaatatc ttcccagcac gtacattgct tgttgcatct gctccgccga
    30541 tatatgccgc tctagttccc cagactgctg catccagttc ttgtgcccgt ctagttccaa
    30601 attccaatag tggatcttct ccgatgacag atttgatacg agctgctttt gtcgcgatca
    30661 acgtttggaa attgaccata ttcaacagtg ctgtttctac taattggcag tgcgctaaag
    30721 gcccttccac ttggatcaat ggttcatttg caaaaactag ttctccttca cgagcagaac
    30781 gaactgttgc tttaaattca aaattttgta aatactccaa aaaaccttca ggatagactt
    30841 ctaatccacg caagtaagca atatcggaat cattgaacgt cagattctct aaataattga
    30901 ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt aaaaggcatt tctcgaaagt
    30961 aacattcaaa aacagcatgt aaatcagctc gacctaattc ccagtatgtc tgcatcatat
    31021 tgatttgata tagatccgta tgcagcgtca aactgtcgtc tgggtaagta ttattcatct
    31081 tgtattactc ctcttggcaa gttttcattt cctacccatt ataacaaaaa aaattaatag
    31141 gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt tacggatatt tcttgctaaa
    31201 aatgaactcc atctgctaaa actatccttt cttattggta tttaacctaa acgagaaaga
    31261 atacttattt tattcaatgt atccgagaca cgaagaaatt agcgttttct atttttctta
    31321 cttgataaaa taagtagatt ttcctactac aatcgtagcc gttaagatac cgatgattga
    31381 acaaatataa ccaaatgtct ctacaacata atcaatggca aaagtttttg aagcaataat
    31441 acgtctttcc ttttttgctt cttatcccca aagaaacgga tataacttat tatgtattga
    31501 atttttttgg aaaggcgaat tatgacaaga tataccttgc gttgaaaaaa ctcgcggaaa
    31561 aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc agctgtcaat agaatggaaa
    31621 caaaaatggc gagacatcaa tcagtctcgc cttgtttttt attgtataca ttagatatgg
    31681 agcaggttac cataaagatg attattagtc gttattaaat agaatccttc gtattttttt
    31741 gttctatcac tacaatatct ttatttggac gtttgaattg tattccggca cgttctagct
    31801 cctgtttcac tcctacatgg actttgacat cttctcgtgt catttttaaa acgtgatgaa
    31861 tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt attgatcatc acgacttgct
    31921 ttttatcata tgtgtctacc aatagcagtt gtgttcgatc taacgaaaat aatttgtaat
    31981 aattaatagc cgtattccca aaaaagaatt ccactacttt aggataaaat gtcttaatat
    32041 ttaaattact attcacaggt gtttcgatta gcttcatctc attcctcttt tctatatagt
    32101 cgatttagat ccaatcttaa gttatgttac catctttcct agcattaagg aatcggttac
    32161 aattgaacct ttaaagacaa aaggatttac cttttatcac ttaatagtat aacataattt
    32221 catgagaaaa aaatacaatt ttttcttgac catctcgtta cgtaaaggat tatgcttaca
    32281 tttgtcagga ggaaaaataa tggagtacac aataaagaaa atgtcagaga tatcaggagt
    32341 cagtccgaga accttgcgct tttatgatga gattggactg ctaaaaccag ctaggatcaa
    32401 ttcttcaggt tatcggattt atggcaaaaa agaagtcgat cgtctgcaac acatcttatt
    32461 ttatcgaaca atggcattca agttggatga tatccaagag gtcttagaca atccatcttt
    32521 tgaccaccag aaagctttaa tcaagcatca gcaaatgttg ctggaaaaac gggcacaaat
    32581 cgataccctc ttgacaactg tccaacagac acttgatatg tacgaaggag gaagaaaaat
    32641 gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag ttgaaagaaa atgaagaatc
    32701 ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag gttacagcat ccaatgaaaa
    32761 atggatgaat atggataaag aaacgtatga agaaatgcaa actgttgaaa agcagatgtt
    32821 atttgatctc actctttact taaaagaacc agaagatgaa agcttagcag accgtatctt
    32881 ccaagctcat aagaagtggt tatcttacag ctggcctgaa tatcagccaa aagcgcataa
    32941 aggactagga atgatgtacg aagccgatga acggttcact gcatattatg acgagcgaag
    33001 tggtcatggt gcagcaaaag ctttaaatga aattatccaa aaaaaggcgt aatcttttac
    33061 atgatccaac aaaaaagtac acgtaaaatg acctaataac aaaacagcag acacccaagt
    33121 tagattttta tgctaacttt tggtgtctgc tccattcttt cacaagtttt ttcatcctac
    33181 tttaaatata ttcatcgttg aataaacgta taaggtattt tcactttatc ctttttttct
    33241 gtaagaaaca attgaaatgc catttctccg caagcttcca gatggtggtc gatggttggg
    33301 aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa tcaccactgg tgtcttatct
    33361 tgcagatagc tttgcaagat gcctgctgca accatatccg aatttgtaaa gacaccatca
    33421 actccaagct ctttaaaaaa gaagcctgct tgttttcctt tctctgcatc tatacaatcc
    33481 caaaaaacaa gtgattcatc aaaagatgga aaaagttgat gacatagctg caaagtgatt
    33541 tttgagttat aacttagcct gccacttcgc cccaacgtga tgcccaattg ctttatacct
    33601 tgctgcttca aataagtaag accttctgta attgattgtt ctcgatcaat aaagacacag
    33661 cttgcctgtt ttcccttgat ttcttcacaa aaaactagag gtccatattt ttgatatgcc
    33721 aataaatcat ctagtgtatt ggcacgagac gtaatgatca atccatcgaa agctttagct
    33781 gcaaattctt caagatacct tcgctctttt tctttctgat agcccgtcgg taaaagtgtc
    33841 actttgtaat cataatcaaa cgctgcttca atgatcccac tgatcaactg atcaaaatac
    33901 ggatgattgg tataaggcag aatcactcca atgcttttcg tttttccata acttaactgt
    33961 ctggcatttg cattagggat ataatcgact tctttcatga ccgcaagtat ttctttcctt
    34021 ttttcctcat ctacatatgg atgtccattg attacacgtg aaatagtaga aactgaataa
    34081 cctgtgagct ttgcgatatc tcgaatgttt gccactgctg cttcgccacc tatacaaaaa
    34141 gattttcttg taaatagtct accacagctg gaccgcattc aaaaaactga tacatttttc
    34201 cttcatgcga aaaactgatc cgctgcccgc cccaatatat atcttttgtc aaattttcga
    34261 tttcattcat tggtatttct tttactatat tcctattcaa cccttgaaaa ctgtacaacg
    34321 ttaatacgga tcccttgaac tcgacaaacg cataaaaata attgttcgtt gtgccatctg
    34381 ttatctctac ataagtattc attgcaagcc ctccttttga tactttgagt atagtgtatg
    34441 aaacgcgtct catagcaagg agtttgtttt gccaaagaaa agttttattc ctctttcatt
    34501 attaccaaag tctcttattt taaattgaat ac
//
LOCUS       DAZKNH010000022        34345 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000022 DAZKNH010000000
VERSION     DAZKNH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.022"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..990
                     /locus_tag="ACG8MG_000980"
     CDS             301..990
                     /locus_tag="ACG8MG_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0375046.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            977..2185
                     /locus_tag="ACG8MG_000981"
     CDS             977..2185
                     /locus_tag="ACG8MG_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0375047.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2479..3750
                     /gene="serS"
                     /locus_tag="ACG8MG_000982"
     CDS             2479..3750
                     /gene="serS"
                     /locus_tag="ACG8MG_000982"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFQ0375048.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4299..5783)
                     /gene="guaB"
                     /locus_tag="ACG8MG_000983"
     CDS             complement(4299..5783)
                     /gene="guaB"
                     /locus_tag="ACG8MG_000983"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFQ0375049.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6288..6983)
                     /locus_tag="ACG8MG_000984"
     CDS             complement(6288..6983)
                     /locus_tag="ACG8MG_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFQ0375050.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7073..8173)
                     /gene="ychF"
                     /locus_tag="ACG8MG_000985"
     CDS             complement(7073..8173)
                     /gene="ychF"
                     /locus_tag="ACG8MG_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFQ0375051.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8182..8373)
                     /locus_tag="ACG8MG_000986"
     CDS             complement(8182..8373)
                     /locus_tag="ACG8MG_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFQ0375052.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8394..9284)
                     /locus_tag="ACG8MG_000987"
     CDS             complement(8394..9284)
                     /locus_tag="ACG8MG_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFQ0375053.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9271..10038)
                     /locus_tag="ACG8MG_000988"
     CDS             complement(9271..10038)
                     /locus_tag="ACG8MG_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0375054.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10050..10763)
                     /gene="rsmG"
                     /locus_tag="ACG8MG_000989"
     CDS             complement(10050..10763)
                     /gene="rsmG"
                     /locus_tag="ACG8MG_000989"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFQ0375055.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(10995..12896)
                     /gene="mnmG"
                     /locus_tag="ACG8MG_000990"
     CDS             complement(10995..12896)
                     /gene="mnmG"
                     /locus_tag="ACG8MG_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFQ0375056.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12913..14310)
                     /gene="mnmE"
                     /locus_tag="ACG8MG_000991"
     CDS             complement(12913..14310)
                     /gene="mnmE"
                     /locus_tag="ACG8MG_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFQ0375057.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14609..14992
                     /locus_tag="ACG8MG_000992"
     CDS             14609..14992
                     /locus_tag="ACG8MG_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375058.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15160..15918)
                     /gene="jag"
                     /locus_tag="ACG8MG_000993"
     CDS             complement(15160..15918)
                     /gene="jag"
                     /locus_tag="ACG8MG_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFQ0375059.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15936..16754)
                     /locus_tag="ACG8MG_000994"
     CDS             complement(15936..16754)
                     /locus_tag="ACG8MG_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFQ0375060.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16751..17107)
                     /gene="rnpA"
                     /locus_tag="ACG8MG_000995"
     CDS             complement(16751..17107)
                     /gene="rnpA"
                     /locus_tag="ACG8MG_000995"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0375061.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17225..17359)
                     /gene="rpmH"
                     /locus_tag="ACG8MG_000996"
     CDS             complement(17225..17359)
                     /gene="rpmH"
                     /locus_tag="ACG8MG_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFQ0375062.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17882..19216
                     /gene="dnaA"
                     /locus_tag="ACG8MG_000997"
     CDS             17882..19216
                     /gene="dnaA"
                     /locus_tag="ACG8MG_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFQ0375063.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19414..20544
                     /gene="dnaN"
                     /locus_tag="ACG8MG_000998"
     CDS             19414..20544
                     /gene="dnaN"
                     /locus_tag="ACG8MG_000998"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFQ0375064.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20773..21015
                     /gene="yaaA"
                     /locus_tag="ACG8MG_000999"
     CDS             20773..21015
                     /gene="yaaA"
                     /locus_tag="ACG8MG_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFQ0375065.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21002..22126
                     /gene="recF"
                     /locus_tag="ACG8MG_001000"
     CDS             21002..22126
                     /gene="recF"
                     /locus_tag="ACG8MG_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFQ0375066.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22123..24069
                     /gene="gyrB"
                     /locus_tag="ACG8MG_001001"
     CDS             22123..24069
                     /gene="gyrB"
                     /locus_tag="ACG8MG_001001"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFQ0375067.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24089..26560
                     /gene="gyrA"
                     /locus_tag="ACG8MG_001002"
     CDS             24089..26560
                     /gene="gyrA"
                     /locus_tag="ACG8MG_001002"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFQ0375068.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26796..27095
                     /gene="rpsF"
                     /locus_tag="ACG8MG_001003"
     CDS             26796..27095
                     /gene="rpsF"
                     /locus_tag="ACG8MG_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFQ0375069.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27145..27657
                     /gene="ssb"
                     /locus_tag="ACG8MG_001004"
     CDS             27145..27657
                     /gene="ssb"
                     /locus_tag="ACG8MG_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0375070.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27683..27919
                     /gene="rpsR"
                     /locus_tag="ACG8MG_001005"
     CDS             27683..27919
                     /gene="rpsR"
                     /locus_tag="ACG8MG_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFQ0375071.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28062..30035
                     /locus_tag="ACG8MG_001006"
     CDS             28062..30035
                     /locus_tag="ACG8MG_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFQ0375072.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30041..30493
                     /gene="rplI"
                     /locus_tag="ACG8MG_001007"
     CDS             30041..30493
                     /gene="rplI"
                     /locus_tag="ACG8MG_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFQ0375073.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30767..32134
                     /gene="dnaB"
                     /locus_tag="ACG8MG_001008"
     CDS             30767..32134
                     /gene="dnaB"
                     /locus_tag="ACG8MG_001008"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFQ0375074.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32914..34206
                     /locus_tag="ACG8MG_001009"
     CDS             32914..34206
                     /locus_tag="ACG8MG_001009"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFQ0375075.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10478 a         6684 c         6806 g        10377 t
ORIGIN      
        1 tcttttgaac aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta
       61 tttatcaaca tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct
      121 ttataactcc gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct
      181 tattcacacc aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta
      241 tctatttctg cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt
      301 atgaaaattt tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt
      361 cacaatgagg ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc
      421 agaacaatcc ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg
      481 atgcaagtag tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca
      541 aaaactactg atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc
      601 aagccattta atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa
      661 atgcagattg ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa
      721 gactctcatg aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc
      781 ggtatcctct acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt
      841 gagcgtgttt ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt
      901 catttaagag ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg
      961 ggagtgggct ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat
     1021 caatttgact tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct
     1081 tttgcttcta ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc
     1141 ttctttgacg aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga
     1201 tttgttctgg aacggccgga attttattat tccctttttc tttctattag atattggtgt
     1261 tttgtattgg cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga
     1321 acttcactac atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga
     1381 cttaagccga gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga
     1441 agacgaacgt aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat
     1501 ccgtacccca ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca
     1561 ctcagaagaa gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa
     1621 atctttagtg gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa
     1681 tttttctgcc tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga
     1741 agcttctaaa aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga
     1801 tggcgatacc gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata
     1861 cggaaaagga gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc
     1921 aacagtcaaa aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg
     1981 tttttatcgt gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc
     2041 aatcgctcaa agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca
     2101 atggacttct ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac
     2161 acaggaagtt attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa
     2221 atgaaaaaca cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa
     2281 atcagcaaag gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag
     2341 gtcggttcaa cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac
     2401 cgcgaatttc agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt
     2461 caaaaaggag gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta
     2521 caagaaaaat tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta
     2581 gatgaaaatc gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat
     2641 gtttctgctg agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct
     2701 gaaatgaaag aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat
     2761 gaaaaattga aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt
     2821 ggaacagatg aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct
     2881 tttgaaccaa aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt
     2941 ggcgcaaaag tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa
     3001 cgtgctgtct acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg
     3061 atcactcctt atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc
     3121 aaagaagatg ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta
     3181 ccattaacta actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc
     3241 actgcactaa gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga
     3301 ctgattcgtc ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat
     3361 tcttacgaag aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat
     3421 ttgccttatc gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact
     3481 tatgacttag aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct
     3541 aactgtgaag atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa
     3601 atccactata ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc
     3661 attttggaaa actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct
     3721 tatatgggga atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga
     3781 gtattaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata
     3841 ccgagaaact ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct
     3901 gagtattaga gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat
     3961 accgagggtc agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct
     4021 cctagttaca aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt
     4081 gattcggagg tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta
     4141 actccaagaa ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc
     4201 ttatttccga aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa
     4261 aagtgttctt tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt
     4321 ggtgcttctt ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc
     4381 atttggataa attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc
     4441 attcctgaac gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa
     4501 gcaacccgac cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg
     4561 aagtaacggt cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt
     4621 ttgaatcgac gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac
     4681 atacttccta gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa
     4741 tatttgatcc ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca
     4801 tcataaatag ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc
     4861 gatcctggtc cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc
     4921 gcttcagccg tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt
     4981 tcttgaattt ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca
     5041 tctgcccctg cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg
     5101 gctgctgcta caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact
     5161 ttctcgatgt ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt
     5221 aatttttcga ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact
     5281 ggggcagtca ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt
     5341 acaaaacgca tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca
     5401 acgattggca caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga
     5461 tgctgtggcg ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt
     5521 cttacttcat ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct
     5581 tgtcttgcca tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg
     5641 attggaatat tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt
     5701 aacacgtgac tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg
     5761 aactttgttt cccagttgga catttttagt accactcctc taatttagtt tatctattaa
     5821 aataattgat tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata
     5881 gaataccaat ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc
     5941 ctgcatcaag taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg
     6001 ggtttattac cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc
     6061 tgcttagttt cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa
     6121 attggactta ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg
     6181 acaagacttt tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc
     6241 tttactaccc ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg
     6301 acggataaac ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca
     6361 gttgccccga taatgataac tactactgga tctaatactg gattgataac tggtggcaat
     6421 agtgttgtcg cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat
     6481 gtcttaccca ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg
     6541 tatttgtaca tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa
     6601 aagccataag ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt
     6661 aatagaccaa gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt
     6721 tttacttctg ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat
     6781 tgacgggctg ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg
     6841 gcttttactt tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa
     6901 atgtactgtt gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt
     6961 gctcgtaatt gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac
     7021 ggtcgtcaca aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat
     7081 taaagcggaa taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa
     7141 cacgtccagc ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga
     7201 cagtttctgc acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg
     7261 gtgctttgat tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt
     7321 aagtagctaa gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt
     7381 cgattcctaa agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt
     7441 cttctgcacg agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca
     7501 cttcttgtac ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta
     7561 cataaagaat tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt
     7621 cagtaaattc gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca
     7681 gtacagctag ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag
     7741 tatagcgttt gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa
     7801 tatcttctag tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc
     7861 gaacaacgtg acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac
     7921 cttctccttt acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg
     7981 gaacagtttt tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt
     8041 ctaccatccc tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg
     8101 cttttgtgat cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag
     8161 cagtcaatgc cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa
     8221 tttcttttca aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat
     8281 tttgatatct gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt
     8341 tttcatttca acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct
     8401 tcatcaaatt gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat
     8461 tcaatctcga tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta
     8521 tccatcaaac gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg
     8581 atttttttct tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg
     8641 cggacagtta aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt
     8701 tttaaaccta acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc
     8761 ttgacaagat ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt
     8821 cctaaacgac tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat
     8881 gcttctgctt cttctaacgg attcagatct tctcgctgca agttttccag cactgctact
     8941 tgcatcatcg cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct
     9001 agtttggacg ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa
     9061 cggcgtacga tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc
     9121 aaagatactt cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat
     9181 ggtagctgga caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga
     9241 aacagtgcat cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt
     9301 tgccaatgct tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt
     9361 tccatgacta ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt
     9421 ttcgcggaag tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc
     9481 atacattgtc agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa
     9541 acgaaccgta ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg
     9601 tatcaaaatc gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg
     9661 gcaatcgatc aaaatataat catactgact gcttacttct gctaaagatc cttttaatct
     9721 tgattctctt gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc
     9781 aggcacaata ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc
     9841 attgactaga acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact
     9901 ggtagcattt ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag
     9961 acaagcacct agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac
    10021 agatattatt tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg
    10081 ggctttcttg ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt
    10141 tgatcagaga cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa
    10201 gcaatcgctt gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa
    10261 aaatagcctt cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca
    10321 accgcacgag cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt
    10381 tctgcccggt catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag
    10441 aaggttattc gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt
    10501 tttaaaggta tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc
    10561 gtaaagtcta cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct
    10621 ttttcatccg taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa
    10681 taaatagcga attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat
    10741 tggcgaaatt cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac
    10801 agattctctc cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct
    10861 taaaaatttg aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa
    10921 tatacacaga agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta
    10981 tttattatta cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag
    11041 accattagga tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt
    11101 gtttctggtt ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct
    11161 gcgtagtcga tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt
    11221 tccatggctt ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct
    11281 tttcgtgtca attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca
    11341 ggacgtctta aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt
    11401 gctaaaaagt cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt
    11461 tcttgttcaa ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag
    11521 cctacacggt gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga
    11581 cggtattcag cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa
    11641 tcatcgatca tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc
    11701 tgaactttca aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa
    11761 ccgcttgtac catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat
    11821 gtcggacgga gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc
    11881 atttctactt tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt
    11941 aaagaagtag ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa
    12001 aataattgat gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa
    12061 tagcgagcgc ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct
    12121 cgaataattt ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt
    12181 ttataggcac tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc
    12241 tcttctgtaa cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg
    12301 aaccgttcga tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga
    12361 gagttattcg gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct
    12421 gttccggctg taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact
    12481 ccatgacaaa caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa
    12541 tttggttctt tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt
    12601 gctcgcaatg ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag
    12661 gttttatcga tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg
    12721 cctttagctg gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt
    12781 attgtcagta ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg
    12841 gcgtgtcctg cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt
    12901 ttgttcctcc ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc
    12961 ttgtacactg tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg
    13021 aacgagatcc acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga
    13081 agctttttct aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc
    13141 tgtttctccg ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt
    13201 tttagctacg gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg
    13261 ttccagctgt gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt
    13321 cgcttccaat aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa
    13381 gatcaagtct gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac
    13441 atcttctgtt tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt
    13501 gacatactct tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc
    13561 ttctcttaac aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc
    13621 cgtacttaac ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat
    13681 tctttgttgg atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc
    13741 atcatattct ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg
    13801 tctcaatgag cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat
    13861 tgctttgtct gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac
    13921 acgaccattc aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc
    13981 tcttaacaat agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc
    14041 tactacatct tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc
    14101 gacaagctga ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac
    14161 ttctgataat cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc
    14221 gccacttaaa cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat
    14281 cgtatcaaat tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca
    14341 aaaaaagtgc ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc
    14401 ttgttcttga ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt
    14461 aaaacaatta gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt
    14521 ggatgtctct ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt
    14581 atctttctta aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga
    14641 aaggagaaaa tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg
    14701 gatcaacgat gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat
    14761 cgtttttgtt gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga
    14821 ccttttctta acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc
    14881 agtaatcaac tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga
    14941 actttttgct tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca
    15001 gacaaaaaaa gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta
    15061 gtgactactt catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga
    15121 aaaactttgt caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg
    15181 ttctactact aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc
    15241 tttgcttaat gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac
    15301 aggctgccct gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc
    15361 ttggcgcttt tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa
    15421 acgatggatg aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc
    15481 aatcagtatg ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct
    15541 ttccattttg acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc
    15601 caattccgtt aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag
    15661 aatcgtttcg ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac
    15721 agtctgttct actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc
    15781 attctttcga cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac
    15841 ttggtctttg gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc
    15901 agcttcataa atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc
    15961 attgctttct taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct
    16021 tttcgaattt tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa
    16081 actacccagt ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca
    16141 ggcatcacga aattcatgat cttcattgat gcattcgatt caatttgact catgctagat
    16201 aaatacgtac ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc
    16261 gcgttcccta agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata
    16321 gattgccata acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat
    16381 ggattgacgc cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt
    16441 tccggatctc ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc
    16501 gttttacgca tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc
    16561 agtgtaaata aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca
    16621 gcaaagtaat aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca
    16681 gttccgcaac cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt
    16741 ttgtatttct tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt
    16801 ggactaaatt ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca
    16861 caataaaatc caactccgga tcaatttctt cttttataga ataaactgct gctcggattt
    16921 ttcgtttgac ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa
    16981 ctcgaaaatg aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa
    17041 aagaatctcc cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt
    17101 ttctcattag ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt
    17161 acgattgtcc tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag
    17221 tggcctaagc tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc
    17281 catttttagt actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg
    17341 gttggtatgt tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac
    17401 agacatactt tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc
    17461 gattgttaaa acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt
    17521 tatgcacagg gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt
    17581 aaaaaaagta atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat
    17641 atctgtattt tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac
    17701 ctgtgcatta gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt
    17761 tgtcgattgt gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact
    17821 attgaaaaaa atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta
    17881 tatggtatcc ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc
    17941 gcctgctagc tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca
    18001 gctcgttgtt gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac
    18061 aaaaatcgtt gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac
    18121 tggcgaagaa gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc
    18181 tagtccgcta aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg
    18241 taaaggaaac cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat
    18301 ttataacccg ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc
    18361 aattggtcat caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga
    18421 aacttttgct aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca
    18481 agaatatcga aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga
    18541 agctacgcaa gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat
    18601 cgtactaacc agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc
    18661 tcgctttgca tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc
    18721 gattttacgt aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta
    18781 catcgcagga caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca
    18841 agcttttgct gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa
    18901 aacgttaaaa tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt
    18961 ggcaaagtat tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat
    19021 tgttgttcct agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc
    19081 gaaaatcgga gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa
    19141 aatccagcag ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa
    19201 ctttttacta aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa
    19261 aaaacgttca aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt
    19321 tttttgagtt ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat
    19381 aataaataaa caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc
    19441 tttatgcagg aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt
    19501 ttgacaggtg taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct
    19561 gatatatcaa ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa
    19621 tctactggtt ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct
    19681 gaagaaacat ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa
    19741 gcgaatttta tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa
    19801 agccataacc agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt
    19861 tttgctgtct cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct
    19921 gataattctt tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca
    19981 gtagaacaag cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta
    20041 tctcgctcat taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg
    20101 ttgttcaaaa cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat
    20161 accaatcgtt tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt
    20221 ttagcggcga tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt
    20281 ttatctattc gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta
    20341 gaagaaagct tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg
    20401 gattatatga aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc
    20461 tctgctattc gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg
    20521 attactcctg tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa
    20581 agaaatagaa aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg
    20641 tttatttttg tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg
    20701 ataaatttgt tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat
    20761 gagggatggg cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa
    20821 atgttgaaag aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa
    20881 aacactgtgt tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc
    20941 atgatggttg aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat
    21001 aatgaggctg aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga
    21061 attttccaag aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt
    21121 ggaaagtatc tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt
    21181 gattggctgg actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac
    21241 tcttccatta gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga
    21301 acagaaaaag ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga
    21361 tctttcttta gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg
    21421 ccagatcaat ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag
    21481 aaaccagtac ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat
    21541 tttgtccgaa caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt
    21601 caagcgattg gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca
    21661 gctggaaatc gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat
    21721 ccaagaacaa tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac
    21781 aacgacagtt ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca
    21841 gacttatgga tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat
    21901 cgatttgatt aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag
    21961 cgagctggat gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac
    22021 gttcttaaca acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat
    22081 tttttatgtt caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt
    22141 ttagtagaac gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa
    22201 gccgttcgta aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac
    22261 cttgtttggg aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa
    22321 atccatgtga ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata
    22381 cctgtagata tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta
    22441 catgctggag gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta
    22501 ggatcttctg ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga
    22561 aatgtgtact atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa
    22621 caaacggatc gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag
    22681 acaactgagt ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac
    22741 cgcgggatgc gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac
    22801 cattatgacg gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt
    22861 tttccagaac ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg
    22921 caatatacag acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat
    22981 gaaggtggaa cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat
    23041 gcccgaaaac aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga
    23101 gaaggattga cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca
    23161 aaaacaaaat tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat
    23221 ttcatgaaat tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta
    23281 ctagcttcca gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt
    23341 gctttagaga ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa
    23401 aaatgcgaat tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt
    23461 aatcgagagt ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca
    23521 agcatggaca agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca
    23581 gggtttggag aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact
    23641 gatgccgatg tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac
    23701 atgcgtccta ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta
    23761 aaacaaggga agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa
    23821 caagtcgttg cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt
    23881 ggagaaatgg atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg
    23941 gcccgcgtca gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg
    24001 gatcgagtag aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta
    24061 gatatttaat catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga
    24121 tgtcaatcta accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt
    24181 cgcacgtgca cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata
    24241 cggcatgaac gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt
    24301 cggagatgtc atgggtaaat accatcctca tggagacatt gcgatctatg agtcaatggt
    24361 acggatggca cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg
    24421 ttctgttgac ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat
    24481 cgctacagaa atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga
    24541 tgatacagaa aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg
    24601 aacaacgggg atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt
    24661 agtagcggct atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga
    24721 agtattacct ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg
    24781 aagagcgtat gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga
    24841 gatgccaaat ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc
    24901 aaaattgatc gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga
    24961 tttgcgggac gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc
    25021 cagcgcatcc gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg
    25081 attcaatatg ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct
    25141 tgaaaactat atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga
    25201 aaaagcagaa gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga
    25261 tgaaatcatt gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat
    25321 cgaacggttc gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg
    25381 attgaccggt ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat
    25441 tgaagattta actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga
    25501 attagcagac ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga
    25561 ggtcttaagt ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac
    25621 aaacaatggt tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg
    25681 acgtggtgtc caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg
    25741 ttctacccat gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg
    25801 atacgaaatt ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg
    25861 tatcgattcg agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg
    25921 ccactatctc ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt
    25981 tgctaacatc cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt
    26041 caatgtgcta ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc
    26101 tgtcactttc aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg
    26161 gatacgcctg cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga
    26221 agtattagtg atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt
    26281 gaaaggccgc ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact
    26341 agctggattg accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt
    26401 cattatccgt ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg
    26461 tttgatgaag atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc
    26521 tgcagaacaa acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta
    26581 agctagcaag aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt
    26641 gaatttctct taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt
    26701 gtataattag agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac
    26761 ctaagagccg aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca
    26821 tgtacattat tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg
    26881 atgcaatctt gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc
    26941 gtttcgctta tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt
    27001 ctccatcatc agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata
    27061 tcattcgtca catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga
    27121 aaacgatgag aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg
    27181 aaagatccag atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct
    27241 gtgaatcgta attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg
    27301 atttggcgca aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt
    27361 gttactggac gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg
    27421 acagaagtag tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc
    27481 caacaaagcg gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat
    27541 aactctaatc aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct
    27601 gacccattcg gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg
    27661 aatttgatag gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa
    27721 agtagactac attgctgcta accatattga atatatcgat tataaagata tcgaattact
    27781 aaaaagattt atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc
    27841 taaaaaccaa cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc
    27901 tttcgttagc gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta
    27961 ttcaattcct ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa
    28021 gttatggtaa aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc
    28081 atttatcccc gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct
    28141 taattttacc atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg
    28201 ttcgctattg gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc
    28261 aggcttcgac tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag
    28321 tgattgctta tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa
    28381 tcaaagatca aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag
    28441 aaaaaaagaa aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag
    28501 taaagggtgt tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga
    28561 cgaacagtca gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata
    28621 aattagatga caaagagatt tcttatttga actctttcgt cactacacta gtttctgact
    28681 ggatgaatga ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag
    28741 cacagatgat cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc
    28801 gagcagaagc tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct
    28861 caagaaccct cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc
    28921 gtggaggcga ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg
    28981 gggggaaatc tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg
    29041 ctttgcgtcg gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg
    29101 atatggatgc tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa
    29161 aagcttacat cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag
    29221 aaatccataa gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc
    29281 tgaaaaaaga agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct
    29341 ctcagggcct atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg
    29401 aagagtttcc aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag
    29461 agctagtttc tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg
    29521 aggccacatt attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa
    29581 ctgcacgaac ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag
    29641 tacaatattt attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg
    29701 gaagcgacta tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata
    29761 atagtgttat agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag
    29821 ctttcgtcat tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca
    29881 cgatcaacgt ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag
    29941 ctactcaact ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca
    30001 aagaaaatat gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca
    30061 agatgtaaaa ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca
    30121 aaattatcta atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt
    30181 agcaggacaa aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa
    30241 gttaaaagaa ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga
    30301 agacagccgt ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca
    30361 atacaaaatc aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg
    30421 ctacacaaac gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt
    30481 cgtacaagaa taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa
    30541 aaagacaaac cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa
    30601 tcgatttcag attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg
    30661 agtataataa aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc
    30721 tcattttctt gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg
    30781 gcaggatcga atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt
    30841 tcttgacgca gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg
    30901 tcgtggccat caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat
    30961 tgacgtggtt actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg
    31021 tttaagttat ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta
    31081 tgcgaaaatc gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat
    31141 cgttacgaaa ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg
    31201 cagtatcttg gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt
    31261 actgaactcc acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg
    31321 tctaccaact ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact
    31381 aatcattctt gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca
    31441 aaatgtagga acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga
    31501 atctcttgta aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac
    31561 aggtcagctt tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg
    31621 agcaaatatt tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg
    31681 tcggaaacta gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt
    31741 gattgaaggt aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact
    31801 gaaaaaactg gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg
    31861 agtagaacag cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat
    31921 tgaacaagat gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg
    31981 cggagatgac gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga
    32041 gaagaaccga agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa
    32101 gttttcgtcg ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat
    32161 cccgagagct ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat
    32221 ttaaacagat ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata
    32281 aacggtgttc aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac
    32341 tattttgacg tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc
    32401 cttaaacggt gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag
    32461 aagcagtttt ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct
    32521 ttcttaaacg gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata
    32581 tgagaagcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
    32641 ctctgattca cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa
    32701 atttgagaag caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac
    32761 aacctctttt ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa
    32821 aataaaaata aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt
    32881 tcaggattaa ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg
    32941 caatggggcg atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg
    33001 attgcacggt accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact
    33061 tataaactgc atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga
    33121 aacggcgttg tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa
    33181 ggtgtagaaa caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat
    33241 atcaaattgg accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt
    33301 aaaggaatcg gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac
    33361 ttattagata aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt
    33421 caatttgtaa aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat
    33481 tatgaatacg gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat
    33541 gctttggatg caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc
    33601 gaccagggaa cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc
    33661 ggaagcggtg tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg
    33721 tcacgtgtcg gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc
    33781 cgcgaagtag gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt
    33841 gattctgtag ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac
    33901 tcgatcgatg tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat
    33961 ggcgaactaa tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc
    34021 tatgaagaat tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg
    34081 ccagaaaacg cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca
    34141 acattttctg tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa
    34201 atctaaggat aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt
    34261 tatctatacg ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg
    34321 ataggctgtc tcttgaacac tgttt
//
LOCUS       DAZKNH010000023        32372 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000023 DAZKNH010000000
VERSION     DAZKNH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.023"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1655
                     /locus_tag="ACG8MG_001010"
     CDS             <1..1655
                     /locus_tag="ACG8MG_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0375076.1"
                     /translation="KFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFNLVV
                     PQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTYKIK
                     LREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKSELG
                     IKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNGPFT
                     LEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDVILS
                     GELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVKQVL
                     GDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDSLEF
                     DLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGGWAA
                     DYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIKIIS
                     DDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1725..2072)
                     /locus_tag="ACG8MG_001011"
     CDS             complement(1725..2072)
                     /locus_tag="ACG8MG_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFQ0375077.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2156..3097
                     /gene="opp3b"
                     /locus_tag="ACG8MG_001012"
     CDS             2156..3097
                     /gene="opp3b"
                     /locus_tag="ACG8MG_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0375078.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3098..4141
                     /locus_tag="ACG8MG_001013"
     CDS             3098..4141
                     /locus_tag="ACG8MG_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0375079.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4147..5217
                     /locus_tag="ACG8MG_001014"
     CDS             4147..5217
                     /locus_tag="ACG8MG_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375080.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5207..6160
                     /locus_tag="ACG8MG_001015"
     CDS             5207..6160
                     /locus_tag="ACG8MG_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375081.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6172..7182
                     /locus_tag="ACG8MG_001016"
     CDS             6172..7182
                     /locus_tag="ACG8MG_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0375082.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7467..8288)
                     /locus_tag="ACG8MG_001017"
     CDS             complement(7467..8288)
                     /locus_tag="ACG8MG_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0375083.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8385..8663)
                     /locus_tag="ACG8MG_001018"
     CDS             complement(8385..8663)
                     /locus_tag="ACG8MG_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375084.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8931..10940
                     /gene="metG"
                     /locus_tag="ACG8MG_001019"
     CDS             8931..10940
                     /gene="metG"
                     /locus_tag="ACG8MG_001019"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFQ0375085.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11419..12006)
                     /locus_tag="ACG8MG_001020"
     CDS             complement(11419..12006)
                     /locus_tag="ACG8MG_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375086.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12181..13503
                     /locus_tag="ACG8MG_001021"
     CDS             12181..13503
                     /locus_tag="ACG8MG_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0375087.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13658..14434
                     /locus_tag="ACG8MG_001022"
     CDS             13658..14434
                     /locus_tag="ACG8MG_001022"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0375088.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14427..14996
                     /gene="rnmV"
                     /locus_tag="ACG8MG_001023"
     CDS             14427..14996
                     /gene="rnmV"
                     /locus_tag="ACG8MG_001023"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFQ0375089.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            14989..15873
                     /gene="rsmA"
                     /locus_tag="ACG8MG_001024"
     CDS             14989..15873
                     /gene="rsmA"
                     /locus_tag="ACG8MG_001024"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFQ0375090.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16324..16953
                     /locus_tag="ACG8MG_001025"
     CDS             16324..16953
                     /locus_tag="ACG8MG_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375091.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17077..18186)
                     /locus_tag="ACG8MG_001026"
     CDS             complement(17077..18186)
                     /locus_tag="ACG8MG_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375092.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18395..18817
                     /gene="mgsA"
                     /locus_tag="ACG8MG_001027"
     CDS             18395..18817
                     /gene="mgsA"
                     /locus_tag="ACG8MG_001027"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFQ0375093.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19004..19102
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19140..19676
                     /locus_tag="ACG8MG_001028"
     CDS             19140..19676
                     /locus_tag="ACG8MG_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0375094.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19716..21479)
                     /locus_tag="ACG8MG_001029"
     CDS             complement(19716..21479)
                     /locus_tag="ACG8MG_001029"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375095.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21482..23197)
                     /locus_tag="ACG8MG_001030"
     CDS             complement(21482..23197)
                     /locus_tag="ACG8MG_001030"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375096.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(23976..24200)
                     /locus_tag="ACG8MG_001031"
     CDS             complement(23976..24200)
                     /locus_tag="ACG8MG_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375097.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24391..27168
                     /locus_tag="ACG8MG_001032"
     CDS             24391..27168
                     /locus_tag="ACG8MG_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0375098.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            27249..27668
                     /locus_tag="ACG8MG_001033"
     CDS             27249..27668
                     /locus_tag="ACG8MG_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0375099.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27680..28150
                     /locus_tag="ACG8MG_001034"
     CDS             27680..28150
                     /locus_tag="ACG8MG_001034"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0375100.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28167..28946
                     /locus_tag="ACG8MG_001035"
     CDS             28167..28946
                     /locus_tag="ACG8MG_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0375101.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            28933..29754
                     /locus_tag="ACG8MG_001036"
     CDS             28933..29754
                     /locus_tag="ACG8MG_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0375102.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            29771..30820
                     /locus_tag="ACG8MG_001037"
     CDS             29771..30820
                     /locus_tag="ACG8MG_001037"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0375103.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            30833..31837
                     /locus_tag="ACG8MG_001038"
     CDS             30833..31837
                     /locus_tag="ACG8MG_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0375104.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
BASE COUNT        10529 a         5770 c         6611 g         9462 t
ORIGIN      
        1 agaaatttac atttggtttg gttgcggtat gcggccttgt acttgctgga tgttatggcg
       61 gcagttcagc cacaagcagt agttcagcaa aaagcggttc agcagatgga ggaggcgtat
      121 ttaacttagt cgtgcctcaa gaaatgccta cagctgactt atcagttgca acagatacaa
      181 tcagttttac agcattgaat aacgtttatg aaggaattta ccgtcttgat gaagacagca
      241 aaccacagcc ggcaggagct agcgagttgg cagaagtcag tgaagacgga ttaacttaca
      301 aaatcaaatt acgagaagac gcaaaatggt caaatggtga tcctgttaca gcagcggatt
      361 acgtttatgg ctggcaacga acagtagacc cagctacagc ttctgaatat gcttacctgt
      421 atgcgccagt tgaaaatgca gaagacatta cagctggaaa gaaagataaa tcagaactag
      481 ggatcaaagc agttggtgac tatgaattag aaatcaaatt gacgaaacaa acaccatatt
      541 tccaatatct gcttgctttc ccatcatttt tcccacaaaa acaatccgta gtggaagaaa
      601 atggggatgc ctatgcatct gcaagtgata aagctgttta taatggacca ttcacattag
      661 aagattttga tggaccagga acagatacag aatggactta taagaaaaat gatcattact
      721 gggacaaaga tacagtaaaa ctttctgaaa tcaaagtcag tgtagtaaaa gaatcttcta
      781 ctgcattgaa cttattcaaa gatggccaag cagatgacgt gatcttgtct ggtgagttag
      841 cacagcaaaa cgcaaacgat ccagcttata cttctgttaa agaagcgcgg acaagctata
      901 tcgaattcaa tcaacgtgaa aagaattcac cattcaacaa tgtgaaccta agaaaagcta
      961 tttcttattc tatcgatcgt gaagctttag taaaacaagt actaggtgac ggatcagttg
     1021 tctctacagg attgatccca tcagatatgg taaaaagtcc aacaacgaac gaagactttg
     1081 caaaagaagc tggaaaatta gtttcttatg acaaagataa agcaaaagaa tattgggaaa
     1141 aagcgaaaaa agaattgggc gttgattctt tagaatttga tttgatggct tctgatgatg
     1201 attcatcgaa aaaagtaatc gaatatgttc aaaactcaat ccaagaaaac ttggatggcg
     1261 taacagtcaa accaacacct gttccattct ctgtacgtct tgaccgctca acttcgggtg
     1321 attttgacgc agtactaggc ggatgggctg ctgactatgc agacccaagc agctttactg
     1381 atttgtttgt cacaggtaat tcatacaacc gtggtcgctg gagcaatgca gaatatgacg
     1441 aagcagttaa agcagcttca tcaacagacg ctggcgatga acaagcccgt tgggatgatt
     1501 tccaaaaagc aatcaagatc atctctgatg atatgggtgt cgttccggtg taccaaaaag
     1561 cagaaggtca cctagtgaat gaaaaaatca aaggtatcgt tcaccatgcg gcaggcgctt
     1621 catgggatta caaatggaca tacgttgaag aataatcgtt aataccgtga gcatgagaaa
     1681 aaacgaggct gcggcaaatg tcgcaacctc gttctcatat tcacttatcc taacaaagaa
     1741 aaaagcatga aaatagcaga aagagcaatt agtattccgg caatgatcag ccaaaagctg
     1801 tacataccgt agctggcaga cgagagcgag gaaaattctg gttttttctt tcggttcctt
     1861 gttcgtgcaa gatggaccga tcgctgaaaa tgatcgaaga aacccgaaga aaagacccat
     1921 aaaaaaccgc cgataatcag aaacggcaat gaccataaga atagatcatt agataattga
     1981 atgagtgtaa aggaatggtt gatgagattt tttattaaaa taaccagaat cagtagaaaa
     2041 gctactgcgt aggggataga tttttttttc atttgattgc tcctttattt ttggttcatt
     2101 atctaataat gtagcgaaac atctgtcaga agtcaaaagc tagggggaaa cattagtgaa
     2161 tagttatatc aaatatgtat taaaacgtgt ctttttcatg atcatcacgc tttggttaat
     2221 tgcaacaatc acctttttct tgatgcaatt gctgccgggc acgccatata cgaatcagga
     2281 acgtttgagt cctgaaacaa tcgagatgct aaataaacaa gtcggtttgg ataaaccagt
     2341 tatcgtacaa tatgggattt atctttctaa tttgcttcaa ggagattttg gaatttcctt
     2401 ccaatttaaa aaccagccag tcgcacattt gttagctgga agaatcggac catccttgca
     2461 gttagggtta caggcaatta tctttggaac agtcctaggg acgattttag gaacgatctc
     2521 tgccatgaag caaaatacat gggcagatac atcttctaca ctcgtagcga tattaggtcg
     2581 ttccattcca aactttgtat ttgctgtttt attacaatat atctttgcaa tcaaattaca
     2641 agttttgcca atcgcaaaat gggatggctt tgtgtacaca atcttaccaa caattgcgtt
     2701 ggccatgtcg ccgcttgctg actctgctcg gtttatccgt acggaaatgg tggaagtact
     2761 gcatagtgat tatgtggaat tagcaaaagc aaaaggattg agccgttggg aaatcgcctt
     2821 taaacatggt cttcgaaata gtttgattcc attgatgact ttattaggac cgttagctgt
     2881 agcattgatg accggatcac tggtggtaga aaatatcttt gctattcctg gtatcgggga
     2941 acaattcgtt aagtcaatca caacaaatga ttatcctacg atcatggctg taacgatcct
     3001 ttactcattt atgctgattt ttgttatttt gatcgtggat ctattgtacg gtctagttga
     3061 tccaagaatt cgtgtttcag aagggagccg aggataaatg gaaatgatac caaagcgtta
     3121 tgaaacagtt gcagatatcc ctgcggatga atttcttcca ttacaaaaaa ataccgaaga
     3181 agaacgtgaa cagattactg caccttcttt gaattttatc caagattcat ggcgccgttt
     3241 aaagaaaaac aaagctgccg ttatttcaat ggttttacta ttggtcatca ttttgatctc
     3301 tgtgatcaca atctttgttt ctcctcatga tccaactgcc caaaacgtgg cttatatcaa
     3361 tttgcctcct cgtattccgg gaatcaatat cgatggattg aatggaaaag caatggttgg
     3421 tggtgaactt gttgataaat atgctcaagc aaacgtccct tcaaacgtaa atttcttttt
     3481 aggaacagac ggtttgggac gtgatgtatt aagccgctta tttatgggaa tgagaatctc
     3541 gttgctgatc gcatttattg ctgcattact agatgtaacg attggtgtag cttacgggtt
     3601 ggtttctggt ttgttaggcg gacgtgtcga taacgtgatg caacgttttt tggaaatctt
     3661 atcaggaatt ccaaacctcg ttgtcatgat tttgatgttg gtcgtttttg aaccaggaat
     3721 tttttcaatc gtggctgcta tggccatcac aaactggata ccaatggcac gtatcgttcg
     3781 tgcacaaaca atgaaattaa aagatcaaga atatgtttta gctggattga cattaggtga
     3841 atcaagaatg aaaattgcat tcaaacatat tttgccgaat atctctagtg tcattattat
     3901 tcaaatgatg tttagtattc catctgctat tttcttcgaa gcctttttaa gctttatcgg
     3961 tttaggtctt acgcctccgt cagcttcatt aggaacgatg ttgagcgaag ggtacaagac
     4021 attcctgtat ctgccttatc tattatggat tccggcagta actttatcgg ttatcatgat
     4081 tggctttaac ttacttgctg atggattgcg tgatgccttt gatcctaaaa tgaaagagtg
     4141 aagacgatga cgaaaatttt agaagtgaaa gacttagaaa tctcttttga tacgtatgct
     4201 ggaaaggtac gagcgattcg cggtgtcaat tttcatttga ataaaggaga aacattggcg
     4261 atcgttgggg aatctggaag tggaaaatca gtgacgacca gaagcatcat gcgcttgctg
     4321 tcaagcaacg ccaatataga tgctggtcaa atcctcttta aaggtaacga tatcgttgga
     4381 aaatcagaaa gagagatgca aaaaatccgt ggtaaagaaa tagcgatgat tttccaagat
     4441 ccaatgacct ctttagatcc gacgatgccc attgggaagc aagtcgctga gtcccttaga
     4501 aagcataata aagtttcaaa aaaagaggga caaaaagctg cattagaatt gcttaaatta
     4561 gttggaatac ccgaagctga gaaacgtatc aacagttatc cgcatcagtt ctcaggaggt
     4621 cagcgtcaac ggatcgtgat cgcgattgca ctggtatgtt atccggaaat actgattgct
     4681 gatgaaccta caacagcgct tgatgtgact attcaagcac agattttgga gctgctgaaa
     4741 gaaattcaga caaaaatcga tacatcaatt atctttatca ctcatgacct tggtgtagta
     4801 gcgaatgtcg ctgatcgtgt cgctgtcatg tatggaggaa gaatcgttga agttggaaca
     4861 tcggaagaaa ttttctacaa cccgcaacat ccctatactt ggggactgct tggttcaatg
     4921 ccgacattag atagtgcgaa cgatcgattg tatgctattc ctggttctcc accggattta
     4981 ttgaacccgc caaaaggaga cgcattctat ccaagaaatg aattcgcgat gaaaatcgat
     5041 gcagaacaag aaccaccatt ttttgaacta tcaaaaacac acaaggcagc aacttggcta
     5101 cttgctccgc aggcacccaa agtgacaccg ccagaagaaa tccaacgtcg ctgggctatc
     5161 tttaaagaga aacaaaaaca tacttacggt caaggaggaa ttgctgatgc caaataaact
     5221 acttgaagtt aaaggattga aacagtattt caatgtagga aaaaagaatg aagtacatgc
     5281 tgtaaatgac atcagctttc acatttatga aggagaaaca ttcggtttag ttggagaatc
     5341 tggaagcgga aaatccacta ctggccgtac gatcattcgc ttgaatgaac ctactgatgg
     5401 tgagatatta ttcgacggtc aagatataac gaagatcaaa gataaaaaag gtttgacgaa
     5461 attccggcat gatgttcaga tgatctttca agatccatac gcttcgttga atcctcgtat
     5521 gaaagtcaga gatatcattg ccgaaggaat tgatgttaat ggactagcta aatcatctga
     5581 agagcgtgca aaaaaagtcg atgatctatt gagaactgtt ggattgaatc caagccacgg
     5641 tactcgttat cctcatgaat tttcaggtgg gcaacgccag cgtattggta ttgcacgagc
     5701 attggcagta aaaccgcgtt ttatcatttg tgatgaacca atttctgcac tagatgtgtc
     5761 gatccaagcc caagtggtca atttgttgca agatctccaa aaagaacatc aattgaccta
     5821 tctatttatt gcacatgatt tgtcgatggt taaacatatt agtgaccgaa tcggtgtaat
     5881 gcataacggc ttgttattag agatgggaac aagtgatgaa atctataatc acggtgttca
     5941 tccttataca gaaagtttgt tatctgctat tcctctacca gatccagacc atgagagaaa
     6001 acgcagaaga atcaaatatc aacctgaacc agatgacgga caagtacgtc aacttcgtga
     6061 aatcgcacca gaacattttg tctatgcaac tgagcaggaa gtcccatatt acgcaaaaaa
     6121 actaaaacga caaaaggaat ctcttctggt tgctaactag gagggacata gatgctaaaa
     6181 gcttttaaat tccgtatgta tccgacagaa gaacaaaaac agcaattgat tcgtactttt
     6241 ggatgtgctc gatttaccta taaccatttg ctgaaaaagc ggcagaaaag ttggcaacaa
     6301 acaggagtag ccaatttttc gctcacaccc gctacattaa aaaaagaata cccattctta
     6361 aaagaagtag acagtcttgc tttagcaaat gcacagctta atttggatcg ggcttttcgc
     6421 aattatttca agggacgtgc cagcttccca aagcttaaaa cgaaaaaaag tatgtggcaa
     6481 tcttatacga ccaacaacca aacccgtacg atctatctgg aaaatggcta cttaaagtta
     6541 ccaaaacaaa aagaactaat caagataaat cagcatcggc cagttgaagg atcgattcgc
     6601 tccgctacga tttctgctcg atataatgaa gaattttatg tcgcactact ttgtgatgtt
     6661 tcgccagtaa aaaaagaatc tttagctaaa tggatcggga tcgcctatca tccgaagaca
     6721 ttgatccaaa catctcgacc tctagaagta actttaccaa aatttcacca gacagaagaa
     6781 aagctgcaac atgcacagcg gaaattgaat gtgaaagtcc gctccgctca ccatagaaag
     6841 atcaggttag atcaggcaag caattatcag aaacaaaaga gaaaagtaat ggatctttat
     6901 ctgaaacaaa agaaccagcg agaagattat cttgaacaat tatctgggaa attggtcaaa
     6961 caatatgact acttatttgt agaatctttt ccaaaagaag aggcacatgc tgatttttct
     7021 attcacgact ggcataaatt aataacaaaa ttgcgttata agtcccaatg gtataataaa
     7081 aaatttcttt tgatcaatac cgacggagca gaagaaagca attccgtaag aaagagccaa
     7141 gtagtagaaa aaatgggcag acattcggtt atcaagggat aaaaaatgaa tgaaactgat
     7201 aggagcgtta gggttagcct tgttaataaa aagtcttaat gacttttgtt tctaggaatt
     7261 tttcttttga agaaaagctc actaattatg acaaaccaat aaaacaatag cagaaaaagt
     7321 tcgtacgagc tttttctgct attgttaatt ttttattcag aaagtttacg aatacatcaa
     7381 aattctgaaa caaaaaacgt aactcacaaa gagttgtttt tgcgtttttt cttgaatgat
     7441 taggtctgaa tcttttgctc acaaccttat tcatggtgcc ccggcttgag ctgatagatt
     7501 tttgaaggac ggccgattcc tacaggacgt tcaccgatct catcgaaata ttggagcata
     7561 gctttcttaa aattggaatg atcgatcgtt ttataatcga tccctaagaa tttagcaaat
     7621 acttttcgag cttccgtaat cgtgaaatct ttacctaaaa cttgtagaac ttgcggctcg
     7681 tgttccattt tattgactac acggttgaat gctttcaaga tgatttgact atggtcaaaa
     7741 gccaaagtat ctttgccaga agactctcca gtgactaaat caagcgagat agccacttct
     7801 ccgctagata aatggatctt gttactatga cgttctagtg tgaaccagcg tacttccttg
     7861 gcatcatctc cggcgattag cggttcttct ccaataaagg ctaagtagct gactgtgacg
     7921 acccagcctc gcggatctcg attaggcgta ctgaatgtat gcagttgctc aatattttct
     7981 tttgaaataa ccacaccagt ttcttccttt gtttctctta agacgctttc tccagttgac
     8041 tcatcctttt ggacaaagcc accaggcaat gcccatgaat ttcgataagg atgccctttt
     8101 ctttggatca acaaaacttt cagctgatct tcttctttgt tatagcacat cagtacgata
     8161 tcgacagtca aagagggctt gtcatattct ggacgttcct gtttttgata ccagttaaga
     8221 aattcttctt cacttgcttt atgttcataa tataattttt cttcttcttt agaagcaaaa
     8281 cggttcatct gaccacctcc attgacagta aaagtacttt ttaccttatt gtagcagact
     8341 ctgaaaaaaa accaaggatt tacttgaaaa atctaggtta tcatttaaat atgaaaaata
     8401 aatggaagta tgagattgaa taaaaagaaa agcagataaa acggcaatac tttataccgc
     8461 tcttcacgaa tggcaaagaa gccacaaata acgatatata cgtcaatgat aaaacgcaaa
     8521 atccaagctg ctgtcataaa actgttcaaa tgcccatgga ggttaaaata accatacata
     8581 cgaatataaa aatagctgaa tattaaatgt gctaatgaaa gaaaagcaat actaagtgta
     8641 aaaaaaagat gtttcttctg catgattgtt ccagtccctt actaagatag atagtcttta
     8701 ttataccacg tgtgagggga gaaataaaaa gccgtttttt agtagatatg aaacaaaaca
     8761 aaaacccgca tttattcaac ctattcatcc ttttaaagga tagattgaac gtagatagaa
     8821 gtcgtttatc cagtcttttt cattttgaaa aaaagctaac agcatgcttt ttttatggta
     8881 tactagcaag gaaacgaatt cgcatcatgc gataaaggag gaagagcccc gtggcagaaa
     8941 aggaaacttt ttatatcact acgccaatct attatccaag cggcaaactt catatcggca
     9001 actcgtatac tacgattgca tgtgacgcaa ttgctcgtta taaacggttg atgggctttg
     9061 atgtgtttta tctcactgga gtggatgaac atggtcaaaa aatcgaaaca aaagcagaag
     9121 aactaggtgt aaaaccgcaa gaatatgtag ataaaatggc ggctgatgtc aagaaattat
     9181 ggaaaacgct tgatatcagt tatgataaat tcattcgcac gacagatgat taccataaag
     9241 cagctgttca aaaaatattt gatcgtttat tagaacaggg agatatttac ttaggtgaat
     9301 atgaaggctg gtactctgtt tctgacgaag aattctttac agaaacacaa cttgcagaag
     9361 tgtacaaaga tgacgaaggt aacgtgatcg gtggtaaagc gccaagtgga cacgaagtag
     9421 aattggtaaa agaagaatca tacttcttcc gcatgagcaa atacgcagac cgtctattgg
     9481 aatactatga agaacatccc gaattcatcc agccagaatc tcgtaaaaac gaaatgatca
     9541 acaactttat caaaccagga ttagaggatc ttgcagtttc acgaacaact tttacttggg
     9601 gagtaccagt caagagtgat ccgaaacatg tggtgtatgt atggatcgat gcattatcca
     9661 attatattac tgcactagga tatggctccg aagatgaaag tctattcgaa aaatactggc
     9721 ctgcagatgt tcagatggtc ggtaaagaaa tcgttcgctt tcatacgatc tattggccaa
     9781 tcatgctgat ggcccttgat ttgcctctac ctaaaaaagt atttgggcat ggctggctat
     9841 tgatgaaaga cggaaaaatg tctaaatcaa aaggaaatgt cgtttatcct gaaatgcttg
     9901 tggaacgtta tggtttagat gcactccgtt attatttgct tcgtgctgtt ccgtttgggt
     9961 cagatggtgt ctttacgcca gaagattttg tttcccgtgt caattatgat ctggcaaatg
    10021 atctagggaa cttattgaat cgaacgattg ctatgatcaa caaatattgt gatggactcg
    10081 ttcctgacta tgcctcactt gtcactccat ttgatagtga actgtctacg acggctgcta
    10141 acgttgtcgg acgttaccat gatgcaatgg aaaaaatgga attcaataca gcattggctg
    10201 aaatctgggt gctgatttct cgtgcaaaca aatacattga tgaaacagaa ccatgggtat
    10261 tagcaaaaga cgaagaaaag aaagctgaat tgaatagtgt catgatccat ttagctgaat
    10321 ctttgcgtat cgcagcgata ttacttcagc caatcatgac agaaacgcca gagaagatct
    10381 tcaatcaact aggattggac tctgaaacaa tgaatcttga aggattacac tttggcgaat
    10441 ttccttcagg aacaaaagta gttgcaaaag gtacaccaat cttcccacgt ttagatatgg
    10501 aagaagaagt tgcttttatt caagaaaaaa tgtctgaagg tactcaaaca aacgaagata
    10561 cagtcaaatg ggatccagaa gaaacagaac ttgtttcaac gaaagaaaaa caaatcaaat
    10621 ttgatgtatt tgaaaaagta gaactaaaag tagcagaagt catcaactgt caaaaagtag
    10681 aaggggcaga taagttgctg caattccgac tagatgctgg agacagccaa gaccgtcaaa
    10741 tcttgtcagg aatcgctgaa ttttatcctg atccaagcga attgatcggg aaaaaagtcg
    10801 tcattgtagc aaatctgaaa ccacgaaaga tgcgtggaca aatcagtcaa ggaatgatcc
    10861 tttctgcaga agcaccagat ggctctttac aagtaatcga agcaccaaaa tccatgccaa
    10921 atggctcaga aatcgcatag aaattcaaag ggggtatgac actagttgtg tcataccccc
    10981 tttttccgaa atttgagaag caattttcgt aaattccttc ttattacttg aagccaatca
    11041 tataatacag aaacagagaa ctatccaaaa taaatcctag aaaaagaagt acgagcttcg
    11101 ctgatttttg ttaatttaga ataatactaa taggaatcgt aaaaacagaa aaaaagcata
    11161 atgataaagg gtcgggcaga ttttaattga ttcatctggt actctttttt attgtcctta
    11221 cattcataaa gtttgaatgc aatgacttta ttacatctat atttatatct aacagtagcg
    11281 ttgatgtgtt aattataagt tctatcactt tatgttcaat tagtttgtgc tcttatatgt
    11341 gtgattgaaa atatagataa aaaaacaatc atcggctttg acttcgatga ttgttttctt
    11401 tattttgtaa aaatcctatc acttgatttt atggtaacct tttatttttg tcttcaaacg
    11461 gcttggtaag agagcagtaa gcagtgcaaa tgggatcgaa aagacatacc agaaaatcgt
    11521 acgtccgata ccataatgtt gatagagctt tgtcaacatc acgtgtaaca agatcaaccc
    11581 taaaagcaag gagccagcta taaaccaatg attatcccaa tgcatgactt cactaagtgc
    11641 catcactcca aaaggataga aataaaaata gatcccgact aaggtcatcc gccagttctt
    11701 tgtaaatgtt ccgatattca ataaaaagaa aatcgctgaa agcaaaatac cgataggcgg
    11761 aaaaagccca accaaaaagg aatacaacat cccccacaag atcatcacag gacctttgat
    11821 caatgcaacg attgcgatga acaaagtcag caatattagt ttttgtgata acgttgcaac
    11881 agtcattaat ccaaataaaa ttaatgtgaa ccaatgccaa tgggactgct gttgttcttt
    11941 acgggaaaaa tcgacccggt tggataaata atctgctaat ttcggataag aacgcttaaa
    12001 atccataaac tgttcctcct tcgtggaaaa ggtaggttta gtatacgaaa aataaaaaag
    12061 aaatacatcc gtcttaagag ggagtttttc actgaaagtg gagaaaaaag aatggtaaat
    12121 taaaatattc tcagttatac tgaaaattat accaaatttc aaagaagaaa gaggatggga
    12181 atgaaaggca aactaaaacg agaaatcaat ctgtttggcg cattagctac cgttatggga
    12241 accgtgatcg gtgcaggcgt cttcttcaaa actgctgctg ttactgctag tactcagtct
    12301 acaagtttga cattgctagc ttggcttttg ggtggtttct taacgatctg tgctggattg
    12361 acagtggctg aattagctac agcaatcccg gaaactggtg gagctgtaaa atatattgaa
    12421 gctgcgtatg gaaaattgcc cagcttttta ttaggatggg cgcaaagctt gatttacttt
    12481 cctgctaata ttgctgcatt atcgattatc tttgcgacac aattgacgaa cttattacaa
    12541 ttatctaccg attatctttt gttgattgca gtgatcaccg ctgtttctgt gacagggttg
    12601 aatttgttag ggacaaaagt cggtgcttct gtacaatcag tgacgttgat tgttaaatta
    12661 ataccaattg ctgtgattgt catatgggga ttattgaccc caggacaagg aactgtccag
    12721 ttatttccaa tcgaagctgg aaaagatgtt acttttgtcg aaggattgag cagtgcgttg
    12781 ttagcaacgt tgtttgctta tgacggctgg ttaggtgtcg gtgcaatggc aggggaaatg
    12841 aaacggccgg aaaaagattt gcctaaagca attattcttg gtttgagttt tgtaacagtc
    12901 gtttatctat tgatcaattt tgtttttttg aaaactttgc cgattgatca tcttgccggt
    12961 aatttgaatg cagcttctga agcatcagac gtcatttttg gaggaatcgg tggaaagctg
    13021 gtcactattg ggatcttgat ctcggtatac ggtgcattga acggttatac actaactggg
    13081 atccgcgtgc cttatgcgat ggctttagaa gacgagctac ctttcagtaa acagctgaca
    13141 aagctttcaa agaagtttac ggttccttat gttcctgcgg ttttccagtt agcagtagct
    13201 tgtattatga tgagccttgg ttctttcgac tttttaacag acatgttgat ttttgtgatg
    13261 tggctattca gtttactgat ttttatcggt gtctttattt taagaaaaaa agcgccggaa
    13321 ttgccacgtc catataaagt accactttat ccaatcgttc cgattattgc gattctcggt
    13381 gctattttca ttttaggtat gacgatgatg acgcaaacga aattagcatt gatcggtata
    13441 ggtgttacct tgataggtat cccagtctat tatcaaaaaa agaaaaaacg ctcagaagag
    13501 taaaatattt caacaacaga cagaaaaagt acgaactagg tcctctagat cttcgtgctt
    13561 tttctatttc aataaaacaa gttcttctat tttgattttt gctacttctg caagtagtgc
    13621 ttatgtgcta aagtaagaat gaaaaggaga gataaacatg atatttgatt ctcataccca
    13681 tttaaatgct gaacagttca atgaagatat tccagaaacg attcagcgag cacaagaaat
    13741 gggggtaacg aaaatggcgg tcgtgggctt cgatacgcca acgattgaaa agtctttgca
    13801 attaagccat gactatccaa atatatacag tattatcggc tggcatccga cagaggctgg
    13861 cagttatacg aaagaaatcg aaaataaatt acaagaacaa ttgactttac caaaggttgt
    13921 agcattaggg gaaattggct tggattatca ttggatggaa gacccgaaag aagtccaaga
    13981 taaagtattt cgcagacaga ttgctatcgc aaaagaaatg aacctaccaa tcagtattca
    14041 tactagagaa gcacttgaag acacgtatca aatcctaaaa gaagaagatg ttcgagatat
    14101 cggtggaatc atgcatagtt tcagtggtga ttatgaatgg gcaaaacgat ttcttgactt
    14161 agggatgcat atttcattta gcggtgtcgt cactttcaaa aaagcattag acgtccaaga
    14221 ggctgcagca aatgtaccaa tcgaacgatt acttgtggaa acagatgccc cttatctggc
    14281 accagttcct tttagaggaa agcgaaatga accaggctat actcgttata cagtagaaaa
    14341 gattgctgaa cttcggaatc ttccgacaga agaagtggct gcccaaacat ggacaaatgc
    14401 gcaccgatta tttggtctaa gcgaacatgg ataagatgaa gatccaagag attatagtcg
    14461 ttgaaggaaa agacgataca agaaggctgc gggaagtagt tgatgcagat acgatagaaa
    14521 caatagggtc tgcaatcaat gaagagatcc tgatgcaaat tgaacatgcc caagagactc
    14581 gtggagtgat cgtttttacc gatcctgatt tttcaggaga aaaaataaga aaaatcatta
    14641 tggaagctgt tcctcaggca aaacatgctt ttttgccgcg cacacaagct aaaccaaaga
    14701 aaaagggaag ccttggtgtc gagcatgcaa gtgatgctgc tattttagaa gcattgaaaa
    14761 aggtcgtgac gccagacatt tcccaaacgg ctgtttctga aatcactaga caagaccttt
    14821 tagactatgg tttaatagct ggggcacaag caaaagaaaa acgagaaaaa ctaggtgacg
    14881 agttgcgcat cggttacacg aacggcaaac aattggaaaa acgtttgaag atgtttcgta
    14941 ttacgccgaa agaactcgcc caagcaatga aaatagtgga ggaacaacat gagtgaatat
    15001 cgtgaaattg cgacaccctc tagaacaaaa gagattttat tgaaacatgg tttttcattt
    15061 aaaaaaagtt taggacaaaa ttttctgact gaaccaaata ttttaaggaa aatcgtagaa
    15121 actgcgcaaa tagatgaaca tacgaacgtg atcgaagttg gaccaggaat cggtgcattg
    15181 acggaacaat tagccaagca tgcaaaacaa gtcgtagcat ttgagatcga tgatcgtttg
    15241 atccctgtat tggctgatac gatgcaaccc tatgacaatg tgactattgt acatcaagat
    15301 attttaaaga ccgatttgtc aacagctgtt agagaaacat ttcatgagga gcttccgtta
    15361 aaagtcgtag cgaatttacc ttactatatt acgacaccga tcatgatgca ttttcttgaa
    15421 tcagatttgg ttgtggatga actagtcgtc atgatgcaaa aagaagtagc tgatcgtatc
    15481 tctgctgaac caggaacgaa agcttatggt tcattatcga ttgctgttca atattatatg
    15541 gaagccagtt tagcattcat cgtaccaaaa acagtatttg tccctcagcc aaatgtagat
    15601 tcagcgattt tgaaactgac aagaagagat ataccggcag tcgaagtgac agacgaaaaa
    15661 gcatttttcc gattgacaaa agcggcattt cagcaaagaa gaaaaacgct ttggaataac
    15721 ttgcagcatt cgtatggcaa agatgaccag acaaaagcat ggttagcaaa aagcttggaa
    15781 acagctggaa tcgatccaaa acgtcgtggg gagactttgt cattacaaga attcgccgca
    15841 ttaagcaatg cgatgtcaga aaatatgcag taaaaaaaga ggttgtgaca gaagttgtga
    15901 aaaagctgtc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatgttttt
    15961 cgtttgtttg ggcttattac cgcagatgca agcttttgaa caccgtttaa gaaagaggtt
    16021 gtgggcctgc cgtttaactc taagtcacaa ggaacaattt caaaaaacag cttctcatgt
    16081 ttttgagtta gttggtctta ttacttgatg caagcttttg accactatta tgccgaacaa
    16141 ggagatgtga cacaaatttt gtcacatctc ctatttttgt ttcttgatca aaaaattctt
    16201 atacttgata ctcacactca ttttgtatgg atttagaaaa gaatatgtgt cttgacagtt
    16261 attagtaagt gagttatact aagtcgacat ttatgtgtca agacatatta ggagagagaa
    16321 aaaatgagaa aaaattcagt tcgaaaacta acgatttctg ctttattgat tgggatgggg
    16381 gtcattatcc caatggtcat gcctaaaatc atgattccac cagcttcctt tactttggcg
    16441 tctcatgtgc cattatttat tgcgatgttt tttacaccag gagttgcagt ggctgttgcg
    16501 ctaggtacga catttggttt ctttttaaca acgccggtta tcatcgcatt gcgagcattg
    16561 tcacatcttg tgtttgcgtt gattggggct tggtacttgc aaaagcatcc gagtatcgtt
    16621 ttaaaaaatg gacagtttac ttttgctaat tggcgtttcc aagggttcaa ttttgtgatc
    16681 ggtgtgattc attctttagc agaaatgcta gtagtcagca tcttttactt tattggtaat
    16741 atgcctgaaa catattattc tcaaggatat ttttatacag tattcatctt gatggggatt
    16801 ggtgggctta tccacagttt agttgattat aatattgctt attttatagc aggaacactc
    16861 agcaaatatt ttgatatccc cgtatttacg gcagcaaaaa aagaaaatcg gaaagagtcg
    16921 attcaagggt acgcgaagaa aacgacatat tagacatgat ctctcaaaaa aaaaactctc
    16981 atctagtaat ggataataaa tcctctaaga tgagagtttt ttgtcctaaa aagagctgca
    17041 ccaacatctt ctggcacagc tctaacaggg ggcaatttat ggaagtttga tgacttgttc
    17101 tgtttcttta tcaaagaagt ggcctttgct gatgttgaag gcaaggtcga ctatttcgcc
    17161 tggtttatgg aaatctcttg catctacttt tgagatgaac tcagtagaac cagtttttgt
    17221 gtaaagcatc gtttcagctc caagtaattc ggaaacgaca acttctgatt tcactacggc
    17281 ttcaggtgaa gcttcaaggg ctacttgttc actgtggatg tcttctggac gaataccgaa
    17341 gataacttct tttccttcat aacctttttc aactaatact ttgttttttc cttcaggaat
    17401 acgaagctta agcccatgtc catctgtgat aacaccattt ttcaatgtca cattaaagaa
    17461 gttcatggca ggagatccaa taaagccggc aacgaacata ttgacgggtg tatcataaac
    17521 ttctttcggt gtaccgattt gttggataaa tccgtctttc atgataacga ttcggtcagc
    17581 catagtcatt gcttctgtct gatcgtgagt aacatagatg gtcgttgttt caagacgctg
    17641 gtgaagtttg gcaatctcag cacgcatggc tacacgtaat ttagcatcta aatttgataa
    17701 tggctcatcc attaagaaga cttttgcatc ccgaacgatt gcccgtccca atgccacacg
    17761 ttggcgttgt ccaccagata aagcagctgg ttttcgtttc aagtattctg tcagacctaa
    17821 gatctctgct gcattgtcca cacgtttttt gatgtctgct ttatcatatt tacgtaattt
    17881 caagccaaaa gccatattat caaaaacagt catatgagga tacaatgcgt agttttggaa
    17941 aaccatggca atatcacggt ctttaggtgc cacgtcattc atgacttttc cgccgatatt
    18001 tagttctcct tcagagatat cttccagtcc cgcgatcatt cgcaaagtag ttgactttcc
    18061 acaaccggaa ggaccaacaa aaacgataaa ttcacggtct ttgattgata gattaaaatc
    18121 agttacagaa taattttctg cattgtcata ttttttatat acgtttttca gagccatttc
    18181 taccataata cgttccacac tttctttttt agttttatta actatttaaa gtataaatga
    18241 aagcgttaac taacttcaat gtcatgttga acaagaattt tttctttttt ttgtcaatgt
    18301 gctagtttta agctttaata aatatccata agaaataaaa actgatacac tggagtaaat
    18361 aagtatgtta acaatatcaa aaggagtgtc tcttatgaaa atcgctttga tcgctcatga
    18421 ccgaaaaaaa cctatgatgg tgaaattagc aacagcgtat aaaacaatat tggaaaaaca
    18481 tgaacttttt gctaccggga cgactgggca gaggatcacg gaagcaaccg gcttgccgat
    18541 ccatcggttt aaatccggac cgttaggcgg ggatcagcaa attggagcca tgatttcgga
    18601 ggatcagtta gatttggtga tttttcttcg agacccgctg gctgcacagc cccatgaacc
    18661 agatgtaaca gcacttatcc gtttatctga tgtctatgag attccgttag ctacgaatat
    18721 cggaacagct gaggtgctac taagaggact ggaagccggc ttcctagatt ttcgaagaat
    18781 cgtccatgaa atagacaagc atccactttc attttgatgg ataagcgtat ggaaaaataa
    18841 cgtttaatgt tcgcctatat gtagctataa aagctaaaca aactgataat atgcgatata
    18901 atacgaacgt attggataat ataaacaata atattagttt ttcgtatttg cagtaaatgt
    18961 gaaaatatgc tatagtttct gtgggaaaag atcccaaaaa taatacagag tagaaaataa
    19021 agcgttaagt gctaagagga tgggatgttg cctcttggac gaaggacgta atgttcgcgg
    19081 ttttattttc gcattcgctg catgatacga tgtagcagct cttaaaggag atgctaaaaa
    19141 tgaagaaaca ccggttagac gcacgtatga ttgctattat gggcttattg attgcattga
    19201 tggtcacttt gtcccgactg gtagcaatcg agaccccatt tattaaaatc agtgtcacat
    19261 ttattccaca agtgatcatg ggcattcttt ttggtccttt ttggtcaggg ataggcgcag
    19321 ttttagctga tttagtagga atggccctct tctcgaaatc tgcattcttt attggattta
    19381 cattgaatgc atttattgaa ggagcaatct acggattctt tttctatcga aaagagatca
    19441 cttggaaaaa tgccattctg gctacgctta gtgtcacgct gatcatcaac ttatttttga
    19501 caccattatg gcttgcgttg atgtaccatg tgccattgtt cagctgggtc gtttgggcgc
    19561 cccgcctgtt aaaaacagta atatggttac cgattcaatc aattgccatt tattacgtag
    19621 gacgtagtat tccttacaaa aaaattttaa gaagtttagc gatccacgct aaataaaaaa
    19681 caagcagtct tcccatccca gaagactgct tgtttttatt gtgctgtgaa ttgtgcttga
    19741 tagagccgtc ggtaataacc atctttttga agcagctcat catgggtgcc catttcgaca
    19801 atagctccct gatccatcac taatatctgg tccgcatttt tgattgtgga caggcgatgg
    19861 gcaatcacaa aactggtttt tccttccatc attgctaaga aggcttgttg gatttttttc
    19921 tctgtcaatg tatcgactga gctggtagct tcatctaaga tcaacatagg gggattactg
    19981 atcatcgttc gagcaatcgt caacagttgg cgttgtccat ctgatatctt taacccttga
    20041 ccgccaatct tagtatctaa tttttccggc aaacgttcta caaattcata catataagag
    20101 gcttttaggg catcattgat ttctgcgtca cttgcttccg gattcccaaa acgaagattt
    20161 tctcgcaatg tactgtcaaa tagccaagta tcttgtaaca ccattccaaa acttttacgt
    20221 aatgtatcac gttggataga gcggatgtca tggtcatcga ctgtaattgt tccttttgtt
    20281 gtgtcataaa aacgcatcag caaattaaca agtgtggatt ttccggcccc tgtttttcct
    20341 actatagcaa tggtttgacc tggataagca gtaaaattga aatcttcgat caaacgctgc
    20401 ttggggtcat aagaaaaaga aacatgatca aaagagactt ttcctttgat atttttgggt
    20461 tccaaggcat aggcatctgg caaatcaggc ttttcttctg gctgatcgat caaatcaaat
    20521 gctcgttcta gaccagaaaa agcggtttgc agttgtgtcg tgattccaga aagttcgata
    20581 aatggtttag aaaactgact tgagtagatc gtaaagctgg aaatcacacc aacagtgatt
    20641 gacgaaccac tcttcaatgc caataggccg ccaactaaac caatcgataa ataagcaagg
    20701 tgatctacaa aacgagaaga cgggtttgtc aaggaagaag aaaattgtgc ccgttgtcct
    20761 ttttcatata gatcagcatt tagcagttca aattgctgtt gactagcttc ttcttgctga
    20821 aaagctttca cgattttctg gttaccgatt cgttcggtca caaagcccga gatatcccca
    20881 ataatccttt gctgtctatc gaaatttgtt tgtgaagcac gagcaacgat ccaactcaca
    20941 aggaaaataa ttggtgtaga acaaagaacg accaatgtca gaagtggact tagacgcatc
    21001 ataaaaataa acgaaagaat gatgattgcc actccagaaa ataattgatt gaaggccgca
    21061 gaaattgcaa tggaaatatt gtccatatca tttgtaaatc gactgacgat atttccgtgg
    21121 gagttttgat cataataacg aagtggcaat cgattcagat gagcaaatgc atcttttctt
    21181 aattttgcaa cggataagta agctaaccga ttgcttaaca cttggatgag ccattgactg
    21241 actacgttga ttaaaacgat accaccgaat agaataagta aatggaacag tcgattgaag
    21301 cggacctgtc cttctccaat catttgatcg atactttgac caatttgtaa cgtcatcaca
    21361 actgttgcaa tcccattgat gattcctagt atcacagcta gccaaatgtc ttttgcatag
    21421 gcagtaaggt aaggaatgaa tcggcggagc gtctgcaagg ataatttttt tactttcata
    21481 actattcttc ctcctgtgat gcgacgattt cctgatattc tgctgattga gcaagtaatt
    21541 cttcatgggt accctgagca gcaatcttac cttgatccat cactaagata tgatgtgctt
    21601 cttgaataga gcggacacgt tgggagataa taatcactgt actgccaaga taagtctgta
    21661 gcgccttacg caaatccaag tctgtttgat aatccaacgc acttaaagag tcatctaaaa
    21721 tcagtaatgc tggcttggca atcaaagctc ttgcaatcgt cagtcgctgt ttttgtccgc
    21781 cagagaaatt tttcccattt tccatgactt ttgtatctaa accatttgga agctgacgga
    21841 caaattcttc tgcctgagcg attttcagag cttcccaaca ttctttttct gtggcgtctt
    21901 tcttacccca ttgaaggttt tcacgaatag tcccggtaaa tagtacagaa gtttggggaa
    21961 caagagcaat ttgctgacgg agatgatcca gctgccaaga acgaacatcc attccgtcca
    22021 ttataactgt accggaactg acatcataga aacgcggaat caattgtgtc aaagtcgttt
    22081 tcccgcttcc ggttggacca atgattccaa ggactgtatt tcgcttcagc tgaaatgtaa
    22141 tattttggag agaaagacca gaatcaggag tataacggaa gtcaacatgg tcaaaagcga
    22201 taattgcttc agaagaaaaa tcgggagtag tactgacctc agcttcaaga atcgaatttt
    22261 ctgtgtctag cacctctttc acccgatttc ctgaagcttc ggcacgtgtg aagatcacga
    22321 cgaggtttga aacaacgatc agtgccaaaa gcatttgatt catataattg atcaaagcaa
    22381 gaacttgtcc ttgttgcaag ctaccaatat ttactttgat cccgccaact gtcagcaaac
    22441 agatgatccc gacattcatg atcaaggttg tagcaggtga taatagggca gagatattgg
    22501 aaacacgtaa ataattgttc gctagatcat ttgtgctttt atcgaacttc tctgtttctt
    22561 tttttgtctg cgcaaatgcg cggatgacac gaacaccgct aagattctga ctaacgtgtt
    22621 cattgagctg atctagtttt tcttgcactt tttggtagag aggaacggtt tttttgatga
    22681 taaaaaataa aatgattgag aaaataggca ataatgctaa gaaaaacaga cctacttccc
    22741 agtcgatcac aaaagccatg accactgacc cgatacttaa aaacggtgct ctgatcaata
    22801 aacgaatgac cattgctaaa gctagctgaa gctgattgat gtcgtttgtc attcgggtga
    22861 tcaaggtatc tgttccaaaa tgattcaact cgttgtgaga gaaagtattg atttttttga
    22921 ttagctgatt acgtaactct gtaccaaatc cttgtgaagc tactgaagca tagtattggc
    22981 aaatgatagc acaaatcaga ccaatcacag acatagcgac catccataag cccatttcaa
    23041 tgatttttcc tcggtcattc atttttagtc cattatcgat caaggaagcc atcattaaag
    23101 gcaggacaag ttcgaagaca gcttcaagaa atttgaagaa cgggcccaaa atgatttgct
    23161 tacgatagtc ttttgcataa cgtaataact gaatcatatg attcctccta ttatttattt
    23221 gtccacaatt gaaaaaacaa gtactaccac attgtagctt aggaaattta gaatgaaaag
    23281 agaacaagcc ttttcataaa tgaaagtttt attatgaaga agctttagaa attgaataga
    23341 ctattttttt taaaaaaaag aaaatgagaa aacatgccag tctcttcatt cataggaacg
    23401 tctattttac ttaatttttc tataaatgaa ttctgcataa aaaatatatc aagtaaatgt
    23461 ttatagatga aactctattt acttcttttt taatttgttc ttgcaatctt ttttgctacg
    23521 taatttttgt aaaaaagtgg gttgagagaa atctaattga ttttcaaaga aggagtatga
    23581 aaatgagaaa aatatttatt cgtttattgt gggtactatc ttagaggaat aaataatagt
    23641 ggagaaaact atggtttggc gcagattcta tcaggtttga tggaacgaag acaacctccc
    23701 gacatcaagt caaaagctac ttgagatttt gatctgaaaa taaaaaaaga aaacaaggta
    23761 ggaacatcaa tgtttctacc ttgttttcgt aatcaatggg aaaaaactaa gagtggtgta
    23821 cgtaatcaat aaaaaggatg gtgacaacag tacctcttta ctctctgaaa gcatcataac
    23881 tcgtttttcc tcattatgcc aataaattgt tgttatacca catataagga tttgtaatag
    23941 ctaaaaacag acaggaaagt tgaaatctgt gaatcttatt ttgtatggaa ggatttatag
    24001 aacgcaaaaa tcaagataaa gatgaataaa aatcctgttt cggctatata ttgcgtatct
    24061 acgccaataa aacgagtttg ttctgtcata gccatataaa gcaaaggcaa aaatacaact
    24121 acagcgagat aagatccgat gatttctcga aaagttttcc aggaagtatg tttcctgttt
    24181 ttatatttat ccgatttcat tgaatagtca tagcctcctt attctttcat atgttgaaat
    24241 tccagtataa aaactggaag gaactgtacg ttttttagaa taaaaattat aagggataag
    24301 taggctgctg tctataataa gatatatttt tcaattcttt attaaagatt ttattgacaa
    24361 aatgaatccg ttttctatga tggaggaggg gtgaataagt tgttgaagaa ggaaatttta
    24421 tgttacttag agaaccagac agcctttttc gatttagaac atatgaacga ggtctttaca
    24481 gcacaaaggt tagctgatca ttttggggta aaaagaaaca cagtcagtca ttatttgaac
    24541 cagttaaatg atgagcaact actagtcaaa atcaattctc gaccagtgat ttatttccac
    24601 aaagaagctt ttgaaaaaca attttttaag ttacgcacga atttttattt ttctatcgat
    24661 gatctcaaag aggaacagcc ttttttcgcg caaccaaaag acttattctc ttttatgatc
    24721 ggatacgacg ccagtttgaa agaaagtatc gaacaaatca aaacagctct ttactatcca
    24781 gatggcggat taccactttt gattacaggc gaaagtggca caggtaaaag ttatttagtt
    24841 aatttggtct atcagtattg tctgcttcat gatctgctag aagattctgc accattcatc
    24901 acttttaatt gtgcacaata tgcagataat cctgaacttt tgactagcaa tttgtttgga
    24961 catgttaagg gagcgtacac tggtgcagaa gaaaataaaa aaggggcttt tgaagcagca
    25021 gatggtggga tcttgttttt agacgaggta catcgcttaa caccagaagg tcaggaaaaa
    25081 ctgttcacct atcttgatca aggtgtgatt tatcgtatgg gagaacctaa tttgtcacgc
    25141 cggatcaaaa cacgtctctt ttttgctacg actgaagaga ttactagtca tttcttaaca
    25201 actttcattc gaagaatacc tatccgaatc gaactacctg cacttaatca gcgtagtcgt
    25261 aatgaacggc tagaactgat ttactctttt tttctcgaag aacaaagaaa aatggggagc
    25321 cctttgtcag tcagtggacc agctttgtca cttttaacaa gtcagaaatt atccggaaac
    25381 ataggagagc tgaaaaatat cgtcaaagtt tcagcagcaa aagcatatgc tgaacagcgt
    25441 gaacagcctg aaatccatgt cacgatccat catttgcaaa aagaactact tgcgcagcct
    25501 attactaaaa atacagcaca gcaaggaatc tatattacgc aagaccagac cttagagcaa
    25561 ttaatggaga agcaacagcc cgaacagcag cgaatcgtta aaagttttga gcaaatccta
    25621 attgatttca agaaaaataa ctgtctgcta tattcttgcg aaggaaaatt aaagcaagaa
    25681 gtgattcaat tatttgattt tttgctgttt gagaccagta gacaagaaaa acacgagctg
    25741 ctggtttatt ggacacaatc tatccgagag acctttcgac aaatggaaac tgcctatcaa
    25801 atcaaattcg atggaaacag tgtctatgcc ttgagttact atctctatca gcgaagcact
    25861 atcaaatggt taccggaaga acatgatatt atccaactaa tcaaagaatt agaacaacaa
    25921 gtttctcagt cttatccatc tagttatcac tatgtccaac gaattctgga attaagtaag
    25981 atcaaattag atattgaaga cacaagtatg gatcggattt tactgacgat ctatctaaaa
    26041 aaagcgaaat ggtcaaaaga aaaacgactg ccaaaagcga tgattgctgc tcacggttat
    26101 gcaactgcta gcagtatggc aaatgtagta aatcgtttat tacaagagga tctatttgaa
    26161 tcatttgata tgccactaga tgtcacacca cagcagattg cttcagaaat ccttgattat
    26221 tgcgaaaaca gcgatgtttc aaatggattg atcatattag tagacatggg atctctgaaa
    26281 gaaatccatc agttttttaa aaaacagtta tccgtaccgc ttttgatttt gaataatgtg
    26341 acgacacctt tagcaatcac agtaggggaa tgcctgcaaa aaaatgtatc attagaagag
    26401 atagcagaag agactgttcg acagatccag ccagaatggc gactgctcta tccagaggaa
    26461 aacaaaccca aagcactcat tactacttgc ttcactggaa tcggtacggc ggtccactta
    26521 agtgaactgc tagaaaaaag cttacctacg gcttgtcagt tgaaaatcat cccatatgaa
    26581 tatcagcaat taaaagataa gaaaaacagt gacccattat tttccatata cgaagtcgta
    26641 ggaatgattg gcaccacaga tcctgatatc accaatatcg actatctttc attagaagac
    26701 ctcatctcgg gggaaaagat gtctgtacta gctgaatggt tggacactac tatgaatgct
    26761 tcagagaagg aaatattcaa tcagcgcatc attcgtaatt tttcattgga aaaagtactg
    26821 gattccgtga ctattctaga tacagagaag gtgatgggag aaatcgatca ttttatgaga
    26881 gaattagagg tgcagttgaa tcgaagcatc agcaatccca aaaaattagc gttgtatgtc
    26941 catgtgagct gtctcattga acgattgatc cgacaaatgc caatcgaaac ctatcaagga
    27001 ttagagaaat ggaagcagtg tcaaaaagaa gggttatctg ctattaaatc tgcgtttagt
    27061 gtcattgaag agcattatag tgtcatgatc cccaattctg agattgccta tatctatgat
    27121 attttgagtg aagatacaga gtttctctcg atagaagaag aattttgaac ataagcaagt
    27181 tgttgccact aattttggca tgcaacttgc ttttttagta tgtgtaagtg aaaagaggag
    27241 atgaaaaagt ggatagagaa attatcttag cttcccatgg aaaatttgct tcagggatat
    27301 tagattcatt agagctgatc tatgggaaaa atcattctat tactgtattc gattgctata
    27361 ctgatgagaa ttttgattta gcggaaacag taaggcagct ttttatgagg tacaaagata
    27421 aagaaatcat tgttctgacg gatctttttg gcggaagtgt caacaacgaa ttcttacaat
    27481 atatcaatcg ggatcatgtc tatctcgttg ccggattgaa tttgccacta ttgattgagc
    27541 tagtatctcg gttggacgct gaagtagaga caactgtttt gatccagcag gtgctaactg
    27601 attcaaaaaa aatgatccag ttttgtaacg agcggctcaa tcagaaaata gaagaagacg
    27661 aattttagga ggaagaaaaa tgattgtatt gacaagagtc gaccatcggt tattacacgg
    27721 acaagtagct ttttcatgga cacaaacaat aggcgcagac tgtattttga ttgctaatga
    27781 tgatgtacct acgaatgaga tacgaaagac aacgatcaaa ttagccaagc cacaaggagt
    27841 caaactagtg atcaaatcaa ttgatgactc gattgccgcg ttgaaaagcg gggtgacaga
    27901 taaatataag ttattcatcg tagtggaatc gatcgaggat gcatataagc tggcagaagc
    27961 gtatccagaa atcaaaacca tcaacttagg agggatcaag gcaaaacctg gtactcgcag
    28021 catcggaaaa gcggtaaata ttttaccaga agaggaggaa cgcttgaaac gattaattga
    28081 caaacaaata gaagtagaaa tccgtcaagt accagcagat aaaaaagtgg atgtaagaga
    28141 agttttgtaa gaaaggaaga acctcaatga ttgtacaagc agtcttatta ggaattgttg
    28201 catttgtcgc acaatcagaa tatgcactag ggacatcgtt gctttctcgt ccgattgtga
    28261 cggggctgtt tacaggaatc gtgttaggtg atgtaaaaac tgggatcatt atgggcgcaa
    28321 ctttagagtt agcatttatc ggatcgttct cagtaggtgc ttcgattcca cctgatgttg
    28381 tgaccggtgg gattttaggg acggcttttg ctattacagc tggagccggt acagaaaccg
    28441 cgcttttact aggtttgccg attgctacgt tgacattgat attgaaaaat atttacttag
    28501 ggcttttgat tccaattatg aaccataaag cagacaatta tgcagaagaa ggaaattata
    28561 aaggagtcga gcatatgcac ttgcttgccg gtatcggtct ttccttgatg cttggtgttg
    28621 tggtaatggt ttcttatatg gtgggaagca atacgatcgg aaatctatta aatatgattc
    28681 ctgacttcgt tcaaaagggg ttgtcagtag ctacagggtt gattccagct cttggttttg
    28741 cgatgcttgc tcgtttgctg atcaacaaac aagttgcacc atattttttc ttaggttttg
    28801 ctatagcttc ttacttagag attcctgtga ccggagtagc tatttttggt gcgattttag
    28861 cagttgtggt agtcaatatt atgaatgtcc gtcaactacc acttcaaaca actgcagggg
    28921 aggtagaaga agatgaagaa ttctgagatc aaaaacagca aaataacgaa aaaagagcta
    28981 aatcacgtat tttggcgttc ttttcaaatg gagttttctt ggaattatga acgtcagatg
    29041 aatatggctt atgtttatgc aatgattccg attttgaaaa agttatatca gtcgaaagaa
    29101 gaaatggcag cagctttgaa acggcatcta gagtttttta acacgacccc acatatcgtt
    29161 acactgattt taggaatcac ggctgcaatg gaggaagaaa ataaggatga tcctgaattt
    29221 gatgtgactg cgatcgatag tatcaaaaca tctttgatgg gacctcttgc agggttagga
    29281 gattctttct tttggggaac tttgcgattg attgccacag gtgtgggtac ctctttagcc
    29341 atgcaaggaa atattttagg ccctatcttg ttcattttga ttttcaatat tccgcacatt
    29401 ctgtttcgct atctagcaac aggttgggga tatcgcttag gaacgggttt tttgaaaaag
    29461 atccaggcaa acggtatgat ggaaagtttg acattaggcg cgtctattat tggactgatg
    29521 gtcgttggag caatgactgc cacgatgatc accatcaata ttcctttaaa aatcggttca
    29581 ggagaaaatg ctgtgactgt tcaaagtatt tttgatgata tcgttccaaa tatcttatgt
    29641 cttggagcat ttggcatagt gttttatcta ttgaagaaag aagtgaaacc attaacgatc
    29701 ttgatcggac tagctttctt tggaattttt ggttcgtgga tagggatttt ttaaaaaagg
    29761 agcagagaaa atgtttacga tgcaagatta tatctatgaa gaaaaggaag tactaagtac
    29821 gatcttgaaa aagaatgatt tttcgacaag agaacatatg aaaaaaacag tgaatttact
    29881 gatcttagca acgggttcat cttataacgc ttgtttagca gcgaagccag cattggaatc
    29941 ttatggtgat cttactgtcg atattcaaga acctttttat tttttcaatt atggaaaact
    30001 ttctccttca atcgatactg tgattgctgt gtcacaaagc gggaaaagtg cttctactgt
    30061 tgaagcagta aaaatgatac aaaaacaagg gttacctgtt gttgctatca caaatgacgt
    30121 gcaaagtcca cttgctttag aagctgcaca aattattgat ttaggtgccg gggtagaatc
    30181 agtaggcttc gttacaaaag gctattctgc gactgtttta cagttactac tcttagggat
    30241 aggaattgga ataagtaaaa ataaaatcag caaaaaaata gaacaagact acatgcaaca
    30301 gttgcgcaaa ataatcaacc atttaccagc gattattcaa aaaacggaag aattttttga
    30361 tggatatcaa agtttatttc gtttagcaca gcgtttcgtc accattggat atggtccaaa
    30421 ctggggaaca gctaaagaag cagagacgaa attgactgaa acaatccgcg ttccgtcaca
    30481 aggatttgag ttagaagctt atatgcacgg cccatacctg gaggcagatg cttcgcattt
    30541 gctattcttt attgaagggg aaagtgtaaa taaggaacgt tcgcaaaagt tgcaaagata
    30601 tatgagtcgg tacgttggag agactctcac cattaccaca aaaaaagcaa gaaacgagaa
    30661 aacacttgga ttagcaatag aatgtgatga atatctgtct gtattagcgc ttgtcgttcc
    30721 gtttcaacta ttcgcttata aaaccgcagt agctaaaggt attgacttga acaaaaagat
    30781 ttttgaagac tttgatacag tcttgaaaag taaattataa gaggtggaaa tcatgctgaa
    30841 attcaatgaa gaagaacaaa tcaaagcaat aaaaggcgca ctagctttac gtccgcaagt
    30901 agaagaaatc atcgataaac tttatgcgga aaaatttgac gctgtctatt atctaggaat
    30961 cggtggaacg tatgcttcct cgatgcaagc tgtcacttat atgaatggga aaagcaactt
    31021 gcctgtcttt gtacagcatg ctgcagaata ttacactact ggaaacaaaa gattaacaaa
    31081 agattctatc gttattttgt cctctgttac cggtacaact caagaagtag tgaaagcagt
    31141 agaacaaatc aagaaagtag gagcgacact gattggattt atcgataaag cgaacagtaa
    31201 gttgtctcag ctatgcgatt ttgtggtcac ttatccagct cctggaacag aacaaatcaa
    31261 attttttatg gcagcagatc gattgatgta tttgaatgga gaattcgaag cttattcaga
    31321 atactatgaa cagctcgaac gttatttacc aacagggtta gtggaagcag agaaaaaagc
    31381 agatgcattt ggcttatcat ttgcagaaaa acatcgacgt gatagtatgc attattttat
    31441 tggagcaggg aatcaatggg gagctgttta ttcatatgcc atgtgttact gggaagaaca
    31501 aagctggctt ccatcgaaat ctatccatgc tgccgagttt cttcacggga cgttagagat
    31561 cgtagaagaa acaaccccag tcacattatt tttaggcgaa gatgaacaga gagtgttatc
    31621 tgaacgtgtg gctaaacttt tgccaaaaat ttgcagcaat tatacattga tagacacaaa
    31681 agactatcca gtagaaggta tcagtgaaaa atatcgcgga cgtgttttgt ctttcttatt
    31741 gatgcatgta gtgacacaac gcatcgatgc tcatgtggag cagttgaact gccatccatt
    31801 agagatacgc cgctactatc gtcagtttga ttattaggat agactggata aaaagaatat
    31861 aaaaaataaa gagagcagcg ataactgctc cctttatttt tttaaaatat tggcatcaat
    31921 tacgttataa aacacatttt ttgcattatc tacagttcaa cttataatct gtttaatggg
    31981 acatgtgggt gccaaccatt ttttgttatg caatagtact atttagttgt tgcatggatc
    32041 cgtagtaaaa gtccaagtac aacgagttgc gccagtttac atagcgtttt gtatccagaa
    32101 tgttgactaa aacaaaagtt cctatgtcaa ccgtcaacag agacaatctg tccgttgcag
    32161 gacctgacag atttagaaat gttcaatctg tctcttatgt attttaaatc aatactcact
    32221 aggagaagtt tcctaacctc ctaatagtaa tgctggtagt aacattgtca aaactgcttc
    32281 ctcttaggaa caagcaaagc ataacgagtg taaaaataaa atgtagacta ttgtaacata
    32341 tcattgatta tttcaagtaa aaacgataaa ag
//
LOCUS       DAZKNH010000024        32006 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000024 DAZKNH010000000
VERSION     DAZKNH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.024"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..637)
                     /locus_tag="ACG8MG_001039"
     CDS             complement(<1..637)
                     /locus_tag="ACG8MG_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0375105.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLT"
     gene            complement(757..1404)
                     /locus_tag="ACG8MG_001040"
     CDS             complement(757..1404)
                     /locus_tag="ACG8MG_001040"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFQ0375106.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1488..2915)
                     /gene="uxaC"
                     /locus_tag="ACG8MG_001041"
     CDS             complement(1488..2915)
                     /gene="uxaC"
                     /locus_tag="ACG8MG_001041"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0375107.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(2989..4068)
                     /gene="uxuA"
                     /locus_tag="ACG8MG_001042"
     CDS             complement(2989..4068)
                     /gene="uxuA"
                     /locus_tag="ACG8MG_001042"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0375108.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(4071..5702)
                     /locus_tag="ACG8MG_001043"
     CDS             complement(4071..5702)
                     /locus_tag="ACG8MG_001043"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0375109.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            6006..6677
                     /locus_tag="ACG8MG_001044"
     CDS             6006..6677
                     /locus_tag="ACG8MG_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0375110.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(6837..7028)
                     /locus_tag="ACG8MG_001045"
                     /pseudo
     CDS             complement(6837..7028)
                     /locus_tag="ACG8MG_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(7396..7737)
                     /locus_tag="ACG8MG_001046"
     CDS             complement(7396..7737)
                     /locus_tag="ACG8MG_001046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375111.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
     gene            complement(7834..8286)
                     /locus_tag="ACG8MG_001047"
     CDS             complement(7834..8286)
                     /locus_tag="ACG8MG_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375112.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(8293..8841)
                     /locus_tag="ACG8MG_001048"
     CDS             complement(8293..8841)
                     /locus_tag="ACG8MG_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFQ0375113.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(8886..9218)
                     /locus_tag="ACG8MG_001049"
     CDS             complement(8886..9218)
                     /locus_tag="ACG8MG_001049"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375114.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(9232..9489)
                     /locus_tag="ACG8MG_001050"
     CDS             complement(9232..9489)
                     /locus_tag="ACG8MG_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375115.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(9684..10226)
                     /locus_tag="ACG8MG_001051"
     CDS             complement(9684..10226)
                     /locus_tag="ACG8MG_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFQ0375116.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(10247..11338)
                     /locus_tag="ACG8MG_001052"
     CDS             complement(10247..11338)
                     /locus_tag="ACG8MG_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0375117.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(11346..13556)
                     /locus_tag="ACG8MG_001053"
     CDS             complement(11346..13556)
                     /locus_tag="ACG8MG_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0375118.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(13572..16100)
                     /locus_tag="ACG8MG_001054"
     CDS             complement(13572..16100)
                     /locus_tag="ACG8MG_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0375119.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(16152..16562)
                     /locus_tag="ACG8MG_001055"
     CDS             complement(16152..16562)
                     /locus_tag="ACG8MG_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFQ0375120.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(16575..16793)
                     /locus_tag="ACG8MG_001056"
     CDS             complement(16575..16793)
                     /locus_tag="ACG8MG_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFQ0375121.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(16790..17782)
                     /locus_tag="ACG8MG_001057"
     CDS             complement(16790..17782)
                     /locus_tag="ACG8MG_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0375122.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(17861..18772)
                     /locus_tag="ACG8MG_001058"
     CDS             complement(17861..18772)
                     /locus_tag="ACG8MG_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0375123.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(18791..19135)
                     /locus_tag="ACG8MG_001059"
     CDS             complement(18791..19135)
                     /locus_tag="ACG8MG_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375124.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(19155..19433)
                     /locus_tag="ACG8MG_001060"
     CDS             complement(19155..19433)
                     /locus_tag="ACG8MG_001060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375125.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(19792..20292)
                     /locus_tag="ACG8MG_001061"
     CDS             complement(19792..20292)
                     /locus_tag="ACG8MG_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375126.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(20293..20838)
                     /locus_tag="ACG8MG_001062"
     CDS             complement(20293..20838)
                     /locus_tag="ACG8MG_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375127.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(20835..22091)
                     /locus_tag="ACG8MG_001063"
     CDS             complement(20835..22091)
                     /locus_tag="ACG8MG_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0375128.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(22452..22763)
                     /locus_tag="ACG8MG_001064"
     CDS             complement(22452..22763)
                     /locus_tag="ACG8MG_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375129.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(22786..24261)
                     /locus_tag="ACG8MG_001065"
     CDS             complement(22786..24261)
                     /locus_tag="ACG8MG_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFQ0375130.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(24710..25162)
                     /locus_tag="ACG8MG_001066"
     CDS             complement(24710..25162)
                     /locus_tag="ACG8MG_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375131.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(25164..25529)
                     /locus_tag="ACG8MG_001067"
     CDS             complement(25164..25529)
                     /locus_tag="ACG8MG_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375132.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(25628..29911)
                     /locus_tag="ACG8MG_001068"
     CDS             complement(25628..29911)
                     /locus_tag="ACG8MG_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFQ0375133.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(30058..30297)
                     /locus_tag="ACG8MG_001069"
     CDS             complement(30058..30297)
                     /locus_tag="ACG8MG_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375134.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(30387..30629)
                     /locus_tag="ACG8MG_001070"
                     /pseudo
     CDS             complement(30387..30629)
                     /locus_tag="ACG8MG_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(30742..30993)
                     /locus_tag="ACG8MG_001071"
     CDS             complement(30742..30993)
                     /locus_tag="ACG8MG_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375135.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         9709 a         6743 c         4562 g        10992 t
ORIGIN      
        1 tagttagttt ataacatatt aaataacaaa taagtgcaca tatcgaaaat attcccgcaa
       61 caattgtaaa agttgatcca ccacgaacta tttgagcgcc tgaatcatct cttgtataaa
      121 taaataatgg tgttaataca ccaatgacca aacttgctaa agtagctcca acatttcgga
      181 atgtcgatag cattgttctt tcttctgtaa tagaagtaat agctgatgcc attgatccat
      241 aaggaatatt tatagatgtg taaaaaatac tcccccataa tatatacgta acatacatgt
      301 atactacctt aacgctcata gaagctgaaa tcatagaagt ttgatacatt aaaaaacttg
      361 ctaaagcgac tggaccactc attcttaaaa tccacggttt aaacttaccc tgcttagttg
      421 ctttcgatct atcaacaata cgccccatag ttacatcagt aactgcatcg ataaatcttg
      481 ctactaaaaa taatgttcca acggcagctc cgcttatccc caatactttt gtataaaaga
      541 tcattaaaaa agaactagca aaaatgaaag taaaatcgtt tccaaaatct ccaaacatat
      601 atcctatttt gtctcttata ccaaattttc tttccataca attctctccc ttctgttgtt
      661 aagcgttttc attctgatga aagtacgtac tatactctga agcgtaaaga gtcagtattt
      721 attttttaca tttcagagta ttatttgttt tttctcctaa taattcttta tctcataaaa
      781 cttatctata tatttactag ctactcgact tacttcttca aaatcacctt tttcaacaga
      841 agccaataaa ttcccaccta caccaactgc aacaactcct acttcaaacc aatcttctaa
      901 gttctctata cttactccgc cagtaggcat tatatttacg tgtggtaatg gagcttgata
      961 ggcttttaca atggatggac caaaagcaga acctgggaat agtttaatga tatctacgcc
     1021 agcagtaagt gcctccttta tctcagtaat tgtcatacaa cctggtaaat aaggaatttg
     1081 atataaatta caaatctcag ctgtttgtcg atcaaaagat ggactaacga tatattctgc
     1141 cccagccata attgcgatac gagcagtaaa cgcatctaaa accgtaccgg ctccgattat
     1201 tacatctttt tgatcagcat attttttagc aatttctttg attatttctt cagcattagg
     1261 aactgtaaaa gttagttcaa tccctttaat tccacccgca ataattgaat ttattgcttt
     1321 aatggcctca tccttatttt tcccacgtac aaccgcaatg actccagccg cttctaactt
     1381 acttaaaatt tctacttttt tcatatctat ctccttcctt tatggataca tactttgtct
     1441 agctgtacta acttacaaag aataaattta agcttttatt tcaagtatta ttttctactt
     1501 ccgatttcaa aaaaattaaa ataattatac gcattacgat aacaaatatt ctccactatc
     1561 tgactaagat acatttcatc atctggaatt cggcctgctt taacccactc gcttagtaat
     1621 tgacataata ctcgacgaaa atactcatgg cgaggatagc ttagaaaact tcgagaatca
     1681 gtcaacatac ctgtaaaatt cgacaacaaa ctttgttgtg ccatcgtagt caactgttga
     1741 cgcattcctt cagctgtatc attgaaccac caagcacatc ccaattggat tttttgaatc
     1801 ataccccctt ggaagctttg cattgctgtt gctagtggaa gccaatcagt tggatttaat
     1861 gaatataaaa ttgtttttgg taagcaccca ctcttttcag cggtatcgaa caatgctaca
     1921 acattttgtg tcaaactagc ttgatccccc atcgaatcac caccgacatc tactccaatt
     1981 tgttccttta atatacttga attatttctt aaagcattta aatgcatttg catcgtccaa
     2041 ccatatcttg tatactcaga catcaaatgt aattgaatac ctgtaacaaa ttgtgcaatt
     2101 tctgataaat ggaaatccgt ttttcctgct aaagctttta ttaacagatt gtcgagtatt
     2161 ttttcatctg ctatttcata aaaataagta ttcaaaccgt gatctgctaa tcgacagcca
     2221 atttgatgaa aatgatccac tcgttggctc aatgcttgct tcaacccatt aaagtctttg
     2281 attggtatag ccgctgtttg actaagtttt aaaatatagt tcgaaaagcc tgatgatttg
     2341 atactccaga ccttatctgg acggaaagta ggtaatacct tgaaagaatt atcctgttta
     2401 gaaagctcag tatgatacca caagtcatct gtaggatcat ccgttgtaca gatggctttc
     2461 acatccatca tctccaacaa ttctttcggg cgataggaat ccgtttgaat tttttcgtta
     2521 gcacgtcgcc aaatttctgg tgcattcttc tcagaaattg tcaaatcaat atcaaagtaa
     2581 cgttgtaatt ccaaatgaga ccattcaaaa ataggattac ctggcgcctt ctccactgtt
     2641 tttacaaaag ctaaaaattt ttcataatca tcacctggac cactgatcaa ctcttcttct
     2701 ataccattag ctcgcatcaa tcgccattta taatgatctc cagctccttt atcatttaac
     2761 catacctgag tcaaattctt gtattgctta ttttctagca aatatgctgg gtctaaatga
     2821 caatgatagt cgataattgg ttggtgtttt gcaaatttat gaaaaagtct tttagctatt
     2881 tcattctcta ataaaaagtt cttatctaaa aacatattta aacactcctt tccttccgtt
     2941 agtttttaga agagaaaatt tttcctctaa ataaactccc ttatataatt atttactcgc
     3001 ccccttttca agcgcttccc aaataccatt taaataaact gcacctagag ctcggtcata
     3061 taatccataa ccaggtctac cggtttctcc ccaaatcatc cgaccatgat ctggacgaat
     3121 atagccatta aatccattat cgtacattgt ttttaatatc ttatacatat ctagagaacc
     3181 atattctgat ttatgcggcg cttcgtaaaa atcgagtcca ttgtcactta aatatttgat
     3241 attacgaaca tgtgcaaaag gaatccgatc ccgttgacaa aattcagata gaatttcata
     3301 gacatcatta tctggtcgtt ctgcaatact tccagtacat aatgtaatac cattagaagg
     3361 agaatccact acattacaca accaatctaa atcgtcacga tttttaacaa tacgaggtaa
     3421 tccgaaaatg gaatatggtg gatcatctgg gtgaactgcc attttaatcc caacttcttc
     3481 acatgtcggg ataattgctt tgagaaaata tgcaaagttc tctcgtaatt tctcttcatc
     3541 aacttcttta taggcttcta ataattgctt aatttgtgat aaccgatgag gttcccaacc
     3601 cggcaattca aaattattag tagaattttc tacttctcta ataatttcct ccggatcagt
     3661 agggatattt tttgaaatat aggctaacgt agaagaccca tcatctaaac gataatccaa
     3721 atccgatttt acccaatcaa atactggcat aaaattataa cagataacct caatcccaaa
     3781 ttctgccaaa ttgcgaatcg tctgtttata attctcaatg tattgatctc gtgttggtaa
     3841 accaattttg atatcatcat gaacattaac actctcaata actttcaagg taagtccatt
     3901 ggcttcgact tcatctttta aagatttaat tcgttctctc ggccaaacct ctccggctgg
     3961 tatatccatt aatgttccaa caattccttt cattgctggg atctgacgaa tttcttgtag
     4021 tgagattttt tcttcatttg ccccatacca tctaaaagtc atttccatag ttactttcct
     4081 ccaacttctg ttatatattt attgattgtt ttacgaactg cttgaggacc tactagcatt
     4141 tcgcaaaaga ttttttcagt tttctttcct accccaactt gatataaatc taaaccaaag
     4201 atttctttat tttctaaaat ttcctgtacc gcacaatgaa tctttgaagg atcttgttca
     4261 cctaatttca atactgataa tttatcctgc aactctacca ataacggatc aggacttggc
     4321 acaaaatttt ccccttgatc atcgactgcc agtaaataac gtaaccaagc agcaatagcg
     4381 ataggaataa atgttagatt ttctgcattt ccgtcttttt cgacatactt tttaattgtt
     4441 tcaccatagc gaatcgctac tttttgagaa gtatctgttg caattcgttg tggtgtatct
     4501 ggaatatagg gattaggcaa acgtttatta attacttcat caataaattg ttttggatca
     4561 ataatgcctg gattttcaac tactggtaaa ccttctaaat aacctattcc tttgattaaa
     4621 ccaaccaaat catcatcctg catctctgct gaaattgatt gatatcctag cagacaacct
     4681 gttactgcca aagcagtatg aagtggattc aagcttgtcg taaccttcat gatgtctgct
     4741 ttatcaaccg tctctctatc tgtcataatc acaccagcct tttccaattt aggccggcca
     4801 tttggaaaac tatcttcaat aactaaataa tgaactacct cagtattagt gaaagcagca
     4861 atattggtat gtttttttgt gtgaatgata tctaaatcct cgagccccat catcaataga
     4921 tcttccttta cattctctga aggatttggc gtaattcgat caatcatact ccaaggaaaa
     4981 gtcacttttt ctttatcctt taaataagca ataaattttt cagaaacata tccttctttt
     5041 tgccaagctt ctgcaatcgt aaaaatacta tcttgaaaac gttgaccatt ctgtgagaaa
     5101 ttatcagtag aaaccatcgc aatcggcaac tctccagcat taaatctgct atataacaag
     5161 gatgttacaa tactcatcgt atgttgagga ttgctaggtc cagatttgat atcagctttg
     5221 actaccgaaa agaattgccc tttagaatct tttaaactgt aacctttttc agtaatagta
     5281 aacgttgtaa actgtaattt aggatcttca aaatatttaa ccactctttg atagtccgct
     5341 atattcttcg gattgcaata taaactatca gccgttactg ccaaaagtct ttttttgagt
     5401 tcaccatctc cactcataac aatttccaaa atgtcattgt tatagtcatg ataagctttt
     5461 tccactactt cttcatcaaa tgtttcacac acaactactc ctgtttctaa ttcattttga
     5521 tctgctaagc attgagcaat ttctgcatga aatcctcgat aaagatttcc tccaccaaaa
     5581 tgaagccata ctggatgttg ctggccttct tgcttaactt ctttgacatc aaaacacggc
     5641 agtagaatct gcatttcatt gaattcttcg atagaatttt gccaattttt aatcgaaacc
     5701 ataattaacc tcctcaaact gatatgttag ttttgataca ttttatattt taaaacccat
     5761 tgtaaccgtt gtcaatataa aaagggaata aacttgcttt tttctaccat atcagaatga
     5821 ttgtattttt tattcagaaa aataaaatac tccttctttt tggactgatt ttatcgaaac
     5881 ttattttttc tttttttcat gtattctttt atttttataa ctagtgtatt acttcaaaat
     5941 ttgatattat atggcatgaa cagaattaaa atatcagtta aaataaaagt taaggagcat
     6001 tgtgtatgac aattaaaact agtctccaaa atcaagctta ccaatctatt cgccgacaaa
     6061 ttatctatgc tgacttagaa cctggaaaaa aagtatcaga aaaaaaatta gaagaaacac
     6121 tttctattgg tcgtacacca atacgtgaag cactgattca actccgacaa caaggtcttg
     6181 ttgatacaat tcctcaaagc ggaaattata tctcattaat tgatttagat tcggccaaaa
     6241 atgctcgatt tgtcagagaa catttggaac gacaaattat gttagagtgt tgtgccaaaa
     6301 tgaataacca aaaaaaacgg attttagaaa ctataatttc agaacaagaa aaagctgtta
     6361 gtcaaaaaaa tgctcgtagt ttttttcata atgataattt attccataaa gcttgttttg
     6421 aaattgcaga gcgagaagaa gtatggcaat ggttagaaaa taacaataca cacttagaac
     6481 gatttcgatg gctacgagta aaaacggaag gattaaagtg ggaaaaaatt atggaacaac
     6541 attatcaact atttgaggca ttaacaaaac aagatccaga agaagctaac tttctaacat
     6601 cgttgcatct tcatttaatg cttaatgagc aaaatattgt tttagctact catccaaaat
     6661 atttcaaaaa tatataactt ttaactcttt ttaaattaaa tggttgttct gatcagaaaa
     6721 atatcgcttg ttttcttatt atgtaatata tttcataaac tattctgcat aacaactatt
     6781 gtttgtactt tttacattgg ataactaatt atgactgctg ataacttttg atattaaact
     6841 agtatctcca atacaaataa aaaatagata tcttgttcat caaaattagt tcgtggatac
     6901 ttaccttcat ttacatcatt cagcggtttc ccattttctt ttattctatt atttcatttt
     6961 ctaatagact tggcttcatt tcgcaatact ttttttcaat aggcatcaaa ttggtgttgt
     7021 ttagatattt agttgtagtt ttgtgacatg ataagtatgc tcctttttct cttttccact
     7081 aatacaacga tcaaagggtg cttttttcaa aaaactagaa aaatcaaaat aagattcgta
     7141 ttttgattct atatatcctg tttcaactac atcttatatt tcacaataga ataaatagcc
     7201 ttttgcatag ctgcttaact tataagaaat tgcaatttac ttcttcaaag tgaaaacgaa
     7261 tatgagtaaa ataaaattat tcttcttaca gataattaaa aatagcgcgt aacaaaaatc
     7321 ggcattcaat cacataatta ggattgatgc cgattactta tattctacgt atagttttag
     7381 tattcttatt ttttatcacc agtaaccaat tgagaacagc cctaaagcag aatagtcttt
     7441 tatctcaaaa cgcttaattt caggcttagg aatatgaata atcttttgag tatattcttt
     7501 ctcttttaat cttactgaag aaataccact gcctatcttt tttacaaata tttcatttgg
     7561 tgtacagact aataaaacaa tatttgtaat caatccttgc actaaagaat taaaaggaag
     7621 actatttaaa acagggtgtg cataactata acatgtgaag tagttatttt gaactttaac
     7681 ttttaaaaac tttaaaaata atcttatttg ttcttctaaa caatcatcta tactcatata
     7741 atcactccac taattaatct ccccaaaaag acacagcatt aagagatata ccaacacaac
     7801 agtgcaaata actctacatc ttccttcgca accttatttc aataaaccat aaaaattatt
     7861 ttttaataga aactgaaaat taaaactaga aaaagtagtt ttaccgaatg taagagcatc
     7921 atcaccatca atatagaaat acattctttt ccagttacat ttaattttta aacaaaattc
     7981 tccccaaaaa ggaacttttt ctagtgaaaa cttggtaatc tctttgtaat taattcttgt
     8041 taaagaataa ttaaacactt catttttact tgttattttt tcattgacta taactagttc
     8101 atctggtaaa aaaattaacc aacaaacatc gacaaacagt aaatgatata atccatttcc
     8161 acctccaagc atattcaata ctggtgaaca tgccttagaa acacgaatat agttatggtt
     8221 taaatagata ttttctttaa acattgcttg tttaaaagcc atatgctctt gttctaaact
     8281 tttcataaac ttttactcta atgcacagct agcagcagct gctgcaattc ctgctacaat
     8341 atttacctta gctaactctt tagctgcagc ttttcctaat tgtttttctg caatttttaa
     8401 taataaatcc gcagcttttt caaaattaaa agcttttaat gcttcaataa aatctccctg
     8461 cgtacctata gcccaagcca ctcctcctcc aggaagagaa ttaatggcta cacatccagc
     8521 atatcgtttc caatcaactt tctccattct aggaacaata atatgttcat aaaagactct
     8581 tgctgctctt ccttgttcgt tatcttgact gattcgttca cgaatagcac caaaatcctt
     8641 tatatggtat acaccatgtt cgtctaaaaa accaactgta tcaaaataaa aagttaattc
     8701 atctactaat ttattcttta tttctaaatc aactgtattc actttttctg tggcatacac
     8761 acttgttcca gtactaacaa aagtacttaa acctaataag aaaacaccta aaacaccaaa
     8821 tattttaatt attcttttca taatttctcc tccataaaaa ataagttact ttttaaatat
     8881 cttgtttatg aattcattcc ccaaaggcct tttaagattg atacagcagt ccgttcactt
     8941 cttttagact tgtcatacaa gtcttttaaa tgatcccata tcagactagt ttgtctatct
     9001 agcttcaatc cattaaacgc taatgaacgg ttcatttttt ctattacttt agataaagct
     9061 acagaaattt ttataccttc acaacgcgca tcttggattt caataatagt atcatcaatt
     9121 agtttttgaa tttcttgcct atcaacatat tcaatcttag aaagagaatc ttttaaagct
     9181 actaatgatt caacgatttc ttttttctct tcagacattt tcttcctcct tctagtgacc
     9241 cgtaaagcca tgtagagcag agtccattga accagtaatt acactatcta caacgccatt
     9301 ccaatttact ctacattttc catttttcag atttgtcggt ccatacggat agcctttttg
     9361 acattcaatg ttcggattat accagatagg attataccat tcagccgcag aagcagattc
     9421 agtcgataca aacaatccca caccaacaaa tccaatacac atcacactat agccaattag
     9481 ttttttcatc tcgtatccat ctcctaaaaa atagaatagt tcttatagtt attcataaga
     9541 catatttttt ccgtttttgt tttatccttt ctgtcctata acaacttttt tcaagtaaag
     9601 aatgacatag tttttcttaa aaaaacagtc tgtttttttc tatttcttcg gtgtataata
     9661 aatatgagtg ttcatttttt attttaatac gcactccgta cataataaat cttgatcgca
     9721 tcaatctttt gagcttcttg atctactttc aattgaagta atacaaattg agtaacctta
     9781 ttcattgttt cttctccaaa taacaaatat tgatccggat tctctatatc ttgtgtcacc
     9841 tggtaaagtt tcccagttgt tttcatttgt acatgcgggt ctacatcaat actattgtct
     9901 ttaatacaac gatctgtcat atagtccctc acagcttgat tgcgtttata aacatctgtg
     9961 aaattactcc attttctagc aaaatctttt aagaaatttt ttgtcatttc atctatttgc
    10021 atttgttgcg ctgtctcgtc ttctaatgaa ccaatttgac tgttactttc agtactttga
    10081 agagttccac ttgtttcttt tactgcttcg attgtttctt ctgtcgttct caattgtgtt
    10141 ttttgttttt cagaagaaaa ccaatgacaa ccagtaagac ctatcaaaca taaccaacta
    10201 aatactaaaa ttatcccttt tttcacgttt ctagtcactg cctttcttat ttcaccttac
    10261 gaaccagact acgaattttt gtttgattgt acgtgcgttg ataccgagcc ataatccgtc
    10321 cttgctcggc attctgttcc acactagaga aatgttgtcc gccatatgaa acatctgtta
    10381 taattccggt gtgaccatag attccaggag acaatgcacc acctgcttgc caattcacca
    10441 catctcctgc ttgtaaatca cttggttttg ggttcatgat tacttgccaa ccataggttg
    10501 tccagttata atcttcccct attaacatcg caaacatgtg tccagaaccc attaactgtg
    10561 ggccacctaa ttgagataca taccatgccg taccaccata acattcccca ttatttacgg
    10621 atcgtccaat gatccgatcc aaaaccttcg cattgccttt actttttcct gcaataagcg
    10681 aagttttttt tgttggtagg ctattccaat gcttgatcgc aaaagtatca tattccgtta
    10741 agttatagga cgttatgata ctcattgctc ctcgataata cggactccct tcggtaatac
    10801 ctgctgccgc atagcctcca cgacctagaa tcaataaaca acttccagga tctttattgc
    10861 ccacacattg attaaagttg ctttggctcg ccaccataaa gccataatag tcataacaag
    10921 cttttaagtt cggaaacttc atatagaaac caccttcaga accaccaatt ccacgtgcag
    10981 aaccttgtgg gtagggacaa ccaggaaacc aggtaatccc aaaaaagttc gtatcgtttc
    11041 ttccagattg tgactgtcca taagctgatt catagcctaa ttgagaaaga atcacactcg
    11101 gtaataactg atactttaca gctgcaccaa ctgctgcttg cgcatgttca tctgtgattg
    11161 gaccacgtcc tttaatctct gaaaaaatcg gtaatccttc ggtatattcc ccattaaaag
    11221 cgacagctag acctgtagct tctcctccat gctcagaaag ttctcctgaa aaaaagaatg
    11281 gcaatagaaa aaagggaaga ataaagaaaa gaagacaacc tagacaccct ttcttcattt
    11341 cttcctcatc ctttcttcga taatggtttt tggactgttc gtccttttgg taaagtaaaa
    11401 tcagttcctg gttttgcgtc caaaatcggc agtgttttag gatccttttc tattttttcc
    11461 gattgctctg ttggcttatc taaagaagaa aatgcaggtt gtaacgattt tggtgcggaa
    11521 atcgacggtt tactttcaga cgaacgttgc cccggttgtt gcacagaacg atttcctgat
    11581 agagtggcta ccgacgaatt aggaacagag tattggttca ataactgctg cattgctgga
    11641 taactcccat ttttcgcctg atttttcact tggcgaatct gttgtgaaag agaaccagca
    11701 gctgttaatt gtggcacggg tacttttcca gaaggcaacg gaatcgattg accaacaaaa
    11761 gcgatacctt tatttaacaa tggttttctt tgattattcc cttgttgctt actcaattgt
    11821 gccaatgatt gtacctgttg ttttggtctt actgaattca tggacgaatt tggaatcaaa
    11881 ccatttgaaa gaccagaatt ccactctgtt ttcgatttgg tagaagacat ttcaggctct
    11941 accccatgat aacgaacatg acgaccacta taaggcaagg cttgatttga acgcccacct
    12001 agttcaaagg gattaaccac ttgcctccct tttaccgatt cttgtggtgt acgtttttgt
    12061 tgaaactttt gaaatccatg tatcgctgca gaagcgagtg gattcgttgc tcgatacaca
    12121 ttccctgcta atcctgcacc tttgattgcc aaattttgtc cagcagttga tagttgacca
    12181 ggcagttgtt tcatttgaga caaagaatga cttgctatgt ttttcgtgtt gtttatagtt
    12241 tgatctgcag ctcctaaccc tagtttcttt aataaatctt tccgttttac aatgagtaaa
    12301 attgatagga gtgctttgac aaacatattt cctaaaaaac caaaaacagt attcattgga
    12361 attaaatcgt ctactatgtt atagatcaaa aatgccacca aaataatcac actgtaaaaa
    12421 gatttttgaa acatcaagcc caaaaaggac tttaaggtat tcatgagcat ataatcaaat
    12481 actggaacaa aagaagcgag taatgaaaaa ggaatcacaa gtaataaaca caacaccaac
    12541 atttgaaaga taaaacgaat catgccaatc actacaagtg gtaatccaat acttagatta
    12601 agcaatggca ccatagaacc aacgactgct ttataaacag ctttagttgg ttgaatatag
    12661 acacgataag ccttcttttt ccctctatct tctcctgcag cagtggtcac ttttgtctca
    12721 atatcagata attcttcact tgacgcttcc ttggctagaa attcttgtgg ttcaatctct
    12781 gcttgataca attcttctaa atttgttgtc ccataattta acaatatata cggatcaaca
    12841 acagccgctt tataataaag ttggcgcact ttttgaatgg cttccttttg tgtcgttggc
    12901 actaaaacac cattgacatc ttttgtcgcc aattcatcta gactatttgt agcagaaaaa
    12961 ataaagcctt ctacctccac ggacaaatca tttaaccctt tggtaaaagc ttctccttta
    13021 ctaggtacgt tactaccata gccaaaccaa accattgaaa aaatgacaac gaagagaaat
    13081 cgaataaaca ccatttttgc gtaatgtttc cctctaaata tacgaagagt aaatagatat
    13141 aacacataca agacgacaat ccccgcacca ataggatcgt atatttttga ataaatagta
    13201 tccgaaaaag tgaaaaactg gtgaattaac tcgttcattg cagctccttc atagagcttc
    13261 tcaatgacaa aatcaaggcc ttgccaaatc aatttactta aactaaagaa catattcgtc
    13321 atcattttta gaaaatctgt gccatttgcc ccaaataaca ttttgcctaa tgaaaattca
    13381 ctatcagata aataagaaga aaaggtatca atatgaaatt tcccaatctc atagatcgat
    13441 tgatccacct gcttttctgc cgcatacacc aatccaccaa caaaaagcag tccgaatcca
    13501 atcgttaaaa aaaacaaaac gcaccatttc ttttccttat gctttatata agccattgtt
    13561 tcacctcatt tctattgcat gattaacgta tcctgttctt ctacttgaga gtgacttgtt
    13621 ttttccgttg tttgtaatgc agcggtcatt tcttcaaaca aactgtgtac attgagtttt
    13681 cctacacgtc catagggatc taacatccaa cactgtcctt taatcatgtg gctcatttca
    13741 gctaagttcg cttcatttac ttccaaacgt aaatgattca aaatcttttc tcgctcactg
    13801 tcttcatcaa aagcaaagac gacaccaaaa ttcccttttt ctttatctga acctaagtca
    13861 ccaataaatt gtgtatctaa cactaattga ttcatctggg aacgccccac acgtgccatt
    13921 gctgcaataa tactacgtcc tgcgttcgaa ttattaaata cccatgcttc tgtaaaataa
    13981 atcgctgttt tcttactact atctcgtttt ccaaacattt cacaaaaacg acctaaacta
    14041 atcatcaaac acaaagactt tcgatccgca tctgtatata gttctggtcg gacattgtca
    14101 tttggtaatt tcaaatcttg gatctctaaa atcgttgttt tggcattaaa gtctaatccc
    14161 gggttttctc cataagaaaa tcctaaacgt aaaatcgaat cttctaccgt caagcgtaaa
    14221 aactcaccat attctcgaac ctctttttgt tccatttgtt ctaaatagtc gaccacatgc
    14281 atcaagccaa cttgttgtcc ggcttctttt tgaactaaaa cttcttggat tccctttaat
    14341 accgcccctt ttaaaaggtg attggtacct aacggactta actcatcaat catatcctgg
    14401 gctacttgtt tcgcatctac tcgggataaa caaacaattg gatctaatac accataatta
    14461 cggggatttg tagcatctaa cgtgacataa ctcatttttt gcaaatgttc tacaaacaaa
    14521 gggtaatgtt tctgataata cgggtcttga atgagtttcg taaaccattt gcgcttttct
    14581 cctttgggat ccacaaataa actttgtaca ttcataaacg aactatacaa aaagagcaag
    14641 ccaactaaaa aggtcttccc tttaccggtt tttccagtta ctgctacatg aggcgcatca
    14701 aacaacgcac ctttgattcc ttccgcaatg gctagaagat tcaaaaagac aaaacgattt
    14761 gacgcataaa ttaattcttc taatggtgtt ttttctcctc gagaaccacc atcccctaat
    14821 gtatcttgcc gaccaatata ccagccggat tccataccta attctgatac tgctgaaaac
    14881 aaccattcgc caactgcttc tacagttgtt cggtgcttcc aatgtaactg ccgtcccaat
    14941 ggttgcccta ataaacattt atagaacaat tctcgttgat cattttttgc acggtggata
    15001 tggataatcg tatgaagttg ttgaataacc gttgcactat tttgacgaac ttcttcaacc
    15061 gtttgaccgt atacaataaa acatgcgatc caatcgacga caggttgccc attatctact
    15121 cgttgcttta aatgttttac taatcctcga acagttaata agcgttcact aggttcttca
    15181 tcattttgaa ccaattcctt atgctctgtt ttgaaccgtt ccgatagcca attcagtttt
    15241 aaaccaatac ctccctgtcc tttcaagcgt ggatagtgaa ttttcattcg caattctgct
    15301 gggaaagaaa gatcctgagc aaattgaaat agatcacaat ttctcatatt ttcaggtgtt
    15361 tccccaatag gcaacaatgc taaataacta ctccctaact catcttctaa ttgaagaagg
    15421 cctttatgtt ccttcggtat acgtacatac gttccttgat tgtctttcag tacttcccaa
    15481 ttattttgtt cttctacttt gtgagccatt cctcgaatat attgcagacg attgagatac
    15541 aacagctctt tttttactaa gggacgaata gaaaagctat aaagtaattg tgtcacttct
    15601 tttacataag gatcactttc agaaaacaaa gtttccaaat catctacttc taatccaaat
    15661 aagcgtgcta aacttcctgc taaatctaac cagattagat ttattacatc atgaaaactc
    15721 tccaccgttg ctttttcttt gactttgatt cctaaaataa atgaaggacg atagacttga
    15781 ccttctcgtt ccaacaatgt cactgtttct tttgcataat acgcaccaat tgcttgacta
    15841 tcgggagaaa atccctttcc taatgtgtca aacttcgtcc gtaaattcat tgttcgtgga
    15901 tacatcacca actccacatc ttcataatct gctaaaagca acagtaaacg agccatcatt
    15961 tcttttattt cctgtgtctt ttttggtgtg ttttttgaaa ttgttcgtgg aaaaacatga
    16021 tagtaggccc agatacaatc ctctccattt aacaaacaat ttttcttgaa tgcttttact
    16081 ggttctttta caagcaccaa tgaatttcac ctcccaataa aaaaggaaca accagttgat
    16141 tgtccctttt tctaatcctc taaataaaca tacttattca caaaaagtaa ttcttccaat
    16201 gcattgtttg ataccttgtc ccagttataa tacacacaat caccattcat catacgaatc
    16261 atataacaaa accaatccca taaataaaaa tgcacttttt ttccatcagg ttttagtttt
    16321 gccagtgcta acacacataa ataggggagt cctatataaa aaacaactcc tgctggtgga
    16381 aaaaaagaaa tgacccaacc aataaatgaa cgtaaaaata aatattcaaa gattacgacc
    16441 aacaaaaaag ttgctgcatt gactaatgca actggaaatg gaagacgcca attgcttgta
    16501 atctgttgta cgcgataagg ttccgcataa acaatcgaat aatcataagg ttcccaatcc
    16561 acttcactat cctcctaatc cataaataat ttttcaatga ttttaccaaa cgcatcaaat
    16621 acacgtgttg gatcatttaa gaagaaataa actgcggcac ctgctgcgat agatattaat
    16681 gcatttccta cttttgattt cataaaattt cgcaatccca gataaacaac caccattatt
    16741 aaaaaatagc caccttgcgt taatataaaa tctacaattc ccttcagtgt catttccgtt
    16801 cttctccttt cctatccaac caattatgtg ttaatttttc aacataatac tgatttgttt
    16861 ttttcgtaat gacaatcgtc atttgttctt tttgtaaaag tgacgtatca ccatctcgaa
    16921 agacgaccgt tgcttttaca ataaaaggtt ttttcgtttc tttataacgc aatactttca
    16981 tctctacttg atcaacttta gctgtttgat tgagtccttc aggttctttc atcatgtatg
    17041 acatatcttt tgcattgttt ttcgcatact tatctaagaa atcttccaca aatccttcaa
    17101 catctttcgc ttccgacgca tcaaccgttt cgtataatgt gtcatcataa gtaatcattc
    17161 gttgtttttc tgtattcttt ggtagtgccg taaaataagg ataagcaata atacgacagc
    17221 ccttgtttga aatagaaaag ggaagattga gtacttgtgt gattgtcaca cttgtttctt
    17281 tcttactctc tggattcatc gagcgaatat cataagttac tttataacta gcagttggaa
    17341 tggtttcaat ttcttttaat ccaataaacg aagcagcagt taattttcga acacctgaaa
    17401 cacttcctgg ttcttgaata aagttttgtg cataaaactc tttttttaat gtttcttgac
    17461 gttgttgttc ttgttcatga ttatcgaatg tattcatata caaagcaaca aaacgattca
    17521 taaaggattc aatttcaggt gtgtacgtta attctgaccg actttgtact tctttctttt
    17581 gtaattgttg gatttcatct ttcaaaaacg acacttgaac cttcgttttc atggattgaa
    17641 aaaatgccaa gccccctgaa aaacaaagaa ataaaaccaa acaccaaatc catttcactg
    17701 ctttttttga atggattttc ttttgcttcg gtactttctc tgtataaact ttttctttcc
    17761 gccgtagttg tcgttttttc ataagctact ccttctagta tgtttttact agtacgtacg
    17821 attgacaacg ataagcttgt ttttctgaaa aatagtccat tcaattaccc tcattttcta
    17881 ttccttttct ctttctttta tatccaacaa atccagcaat acttgtcaac actcctccta
    17941 gaacagaaag tgcaacatgt atttcttcac cagtctttgg taagactttt gtttttccat
    18001 ctgctgtttt gatttcaata ttgccacttg gaagtttctt tacaggaatc gacaaatcct
    18061 ttaccaaatt cccttcacta tcttggatta atggaatacc atcttttaca ccaacaattg
    18121 tttgatcacc tgctactttt aatggggtgg ttggtgttgg aatatgggtc aattcctcta
    18181 cattactagt gacaatcggt acactcattc cttgagaagt attcgtcgta atttcaccgc
    18241 ttggggttac cgatactgtg acttcttttg ctggaacaat aggtttattt tcttttggaa
    18301 ttgcttctga tttttcaaca ggttgtttcg gttcttcttt ttgttcttca ctggtagacg
    18361 acatcgttgg ctgttccgta cttgacgaat cagtattctt ttcagaagaa ctggttgttc
    18421 ctgattgttc cggagttgtg actatacttg aatccgttgt tgttgaacta tgcacacctt
    18481 cttcttcgat cgaatcagtc ggtaaagttg attcaggaaa ctctggttct ggtgtcactg
    18541 gctctttttc aggtttctct gactgcaatt gttcaggttc tgctggtttt tccgttgttc
    18601 ctccatttag ctctgtctca acatctggta atccttcccc tggcaatgtt tctacaattt
    18661 ctgacttagg tgtttctaca gtttcatctg cataaaccaa tgaactccct aattgaaacg
    18721 aaaataatga aattcctagt gttgttacga ctgctaatgt tgtttttttc atgattattc
    18781 ctccaatttt ttatgcaatt cgtgttttgt tcatagacgc cgcactaata ttatctgaac
    18841 gataaaagaa ctcccgtccg ttttcttcta acccaacata tggattattt ttgaataatt
    18901 gaatcaatag ttcactggtg ttgtattttg gatagtttgc aaaaaaataa tactgtgctt
    18961 gtcgcccatc cgaaaattca acaagtaatt cataacgata ccgcttcaat ccttttcgtt
    19021 gttttaaagg atctctatac caacttgtcc atcgttccat tcgtggatag agatagacac
    19081 gatacacata gaacaatgcc ataccacata ccaccaaaaa taataatcgc cacataaaaa
    19141 ttcccccttt tcttctacct ttcttgaatc atcgaacatt cataccctct atagagattt
    19201 gaaaaatcca tcatcaattg ttcatataaa cgttgttgca aatagcgatt gtttcgaata
    19261 cataattctt catagccatc aatcacttga tcttcttttt catagcatct tgtagctcta
    19321 tgacgctcag cttgcatgac ataaccaaac tctgcaccat atcgttctac tgttaataac
    19381 tgttgactac gcaataatgc aatataacaa tcacaaaaat gttcaaccga catttgattt
    19441 ttctcacgtt tgtgaaatag gtattcttta gaaagcacat tattcgttaa acaatctgat
    19501 aaatagcgat aatctggcgg tcggttattt tcatctgtga atttttccca taataaactg
    19561 atttttattc gttcagcggc tagatagtct tgttcgcgtg gataaaattc atcaatcgca
    19621 caaagttttt tatcacctga ataaacaaat ccatataatg cgaaacgaac tcttcctttt
    19681 caattggctc tcctaaaaat tccagtgaca taccatcttc tccttcctaa aaaaaataaa
    19741 aaagcagaaa ccgatttggc ttctactttt ctttcctttt atagctcttt tttacggaag
    19801 aatccgcaaa tgacattttt tcaaaaaatc aatcggcaca agttcacgaa aacttggagc
    19861 aattttggat agaatcactt ccccatcttg taaaatacaa tcataaaaat catcagttac
    19921 ttgatactta cgcttcttac aataattgat aaaatagtag cgatcatttt ctatttccat
    19981 tcgttgttct actaagcgca tacgaacatc atcatagaaa taagccacaa atcgcccttc
    20041 aagttcatgt ttttcaatgg gatactcttc tggtaacaat ctattttttc gtcgtttata
    20101 cattgctgga taccttagaa gaagttcatc taaacgtctg tcagtatatg gtaactgacg
    20161 aatcaacgat tctaatcgtt ctttatacag atagaataat tggcgcaagc aatagtgtac
    20221 cgcctctgga ttttctaata ctgttggatc acgtgaccaa tacgcaccaa cataaatcat
    20281 aatttttttc atttagacaa tcacaccagc aatactaatg accgatccac aataggtaga
    20341 acaagtggca tagcgaccaa tttcctctaa ttctatctca atctcttcta aagaaagata
    20401 tgtctgttct tctgttggct ggcgactcat ttcccaagtg acagtcacca atgaaatttc
    20461 ttccataaga ggtttttcgc tgctaagtgc tgccacacag ttttcatata tcttcatttt
    20521 atcgcagcct tttttaaccg ctaaaatcgc aggtattggc aatgaaaatc gataaaagcg
    20581 aatgaattct agccttcctt tccttcggct aaattccaac tcttcgagtt cttgttcgta
    20641 tcccttacga acttctaata agcgtgcaat ttcttgccag ttctcttttt cctgataacg
    20701 tccagctttt accgcttgtt caatataagg aactcgaaaa cgaaaataag acaaatcaac
    20761 catcttttca tttaatgctt cgattgcagc ctttaaatcc aacaggcgca gccattctgt
    20821 tgtcgtatat gcattcatgc aattttccct cctgacgttc ccatccattc tagatagttt
    20881 tgttgctcgt catttagttc ggcattatca atcccttctt gtacaaagtc tcttcctaaa
    20941 atacgatctg cttcttgtaa caactttaat gtcggagcta cttgtttcaa aaaccagcgt
    21001 tctgaacgat ctaatgagaa tttttctggc tttactgtaa aatccaattc ttccatcgaa
    21061 cgaaataatt ttgcccatcg tttatcaaca atccgtgcat cactttccag tctatcccaa
    21121 acaataagtt ttgcattaat taaaccaacg ccaatctctc ctaaacgttt cggtgaatca
    21181 cctaaaataa atgcttctac tgcttgtgtt gctttctcat tggctaaccg taactcatat
    21241 cgattataaa taccatattt cgttaatgct tctaattctg aaattttttc tcggttcgca
    21301 atttcaaaac gtttctcata aaaattaaaa aagagaggac tctttcgcga tccaaaatac
    21361 aaagataatc cttcacttcg aaaatagcca tcatctaaaa aacaaccgcc aatattctgg
    21421 aatctcttaa agtccatttc taataaacct gctttttgtt tttctagtaa ttcaaagaga
    21481 tcgaaatgtt cttccgtttc caatccccaa agttcatcta acgcaatatc gaatcggcta
    21541 cattgcacac gtgtataacc agcttccaac tttccttgat ttttccaaat ggaatatagc
    21601 catgttcgcc aatctgtttg ttgttcacgc atgacttcct catattcccg gcaaccttgt
    21661 cctgaaaatt gcacaagtac attttctgtt acagttcgtt ttgaatgcca gaacacccag
    21721 atattcccac gaacagaaag acagtcaaaa agatacaagc ccgttggacg tgtttcaaat
    21781 tctgtcagat ccattcgtaa tacacgttga atcactgttt tgtagtctgt cccaaaaaag
    21841 gaaagacgca aataatcaat cattgctcga agatctattc cgcttttcat atacaatcga
    21901 aagcgttcct tgattcgttg ttcaaaatcc tctgggattt ttcttttccc ttgttccatc
    21961 tttctaatgt aatcttttga gcagcccatc aattgcgcaa atcttgcttg tgatagcggc
    22021 tttttatctc cacttatatt atgaattgca cttaacttct ttctgtagct ttttagctca
    22081 atcggtgtca ttttctcgct ccttttataa aataacaaaa tcgatttttt ttaacccttt
    22141 agaaacaccg atatatcaac tagtccccct cgttttttgg gaaaagtagt acaactgctg
    22201 accttagata atcacttgat acaaaagggt ttataaaact tttttctatg tgttatgtta
    22261 tttctgcccc ccatattata tatcgggggg ctttacacaa agaggccgtg cgcaagcgca
    22321 cgacctcttt gtgagctgca cacgctgcta gcgagtgcgc attataggcc catgcttcgc
    22381 agccttaatg cgcaccgcca ggcagagtgc ggctaccaga ctacgtctag agccctcccg
    22441 atagataata actacaaaat taaatgatct aattttgtta tttcactata tttttcagga
    22501 gaaaaaacgc gataaattga caaagttaaa tcctcttcta aatctactcg ataatgatac
    22561 aatcgagtca atcgttctat atcttttttc gttagcaata cttgattaat aacttttcct
    22621 gtaaaaacac gttctttgcg caaaaagcgg taatcagtta cttgttcaat acgattcaac
    22681 caatcataaa acgttctgga agaaagagaa ggattaatca ttcgtattgc tgtagctact
    22741 ctataataac aactctctgc catttaactt cacctcctta gttgattaca aaatatgcgc
    22801 atcagaaaaa cttgttcctt ttggatcttt tgctaaatac atttggcgct ctagttgttc
    22861 ttgtaacaac tgattatcat ctcccatcgt ttgatcttga ctatcctctt ttactgtaaa
    22921 atcatagaca atcatttcag taaatttctc aaattctttg ataaagtgat aatctttgta
    22981 aacaaatggc gcaaaaaact ctacaggtac atcaaatcct ggaaaagcat atccacgacc
    23041 aggttgatct ttgttgtaaa atggcttatt tttaaaatct tctccaaaag tcattaaata
    23101 accaatggga gacaatcgac caacagaaat acggaatgtt aaattatccc gaacaccact
    23161 tggaaaatat tctgcatctg gtcgttgcat tgctaaaata atgtacatcc ctgcttgacg
    23221 accctttaat actaatggct gaatcaattt atcgactcta tttctcgctt ccatatccaa
    23281 cgaaccataa aaagctgccc attcatcaaa aaagataaaa tgcggtggta acccataata
    23341 agcataattt ttccctggtt catatttagg atctttcatg gttttcatat aggcataccg
    23401 ttgattcatt tcttttaatg catttcgtaa acaaataatc atttcttcat ttattttgta
    23461 aaacacatgt cctttgaata catcgacgtc acctaatgct gctaaatctg ccgcttttcc
    23521 atcgcaaata tctactgtcc caacacttaa acaagcttga attaaagaat acaataaata
    23581 ggttttccca ccaccgattg caccagcaat caacatattt ggtgattgat catataccca
    23641 atccaaagtt ttcattaaat gaatacgtcc attttctgct atacattgat caataggaat
    23701 ccgaccagaa tctgaaagga atacataatt gattcgggct ttttctcgtt cttcacgaat
    23761 taaatcacgc aaaaatactt cagataacaa atgacccatt tttaaaaatc gttcgtgaaa
    23821 acgcccacca tctaaaggaa aagaaaccac taattcttgt ttcttttgat aaaaataaac
    23881 ttttggtaat ctcatttttt cccttgtttg tttctcacta acttgtttct ttgtataaac
    23941 aaaccgattg tcaattaaca ttcgaacaag taactgacgc caataaacag aggcaaaata
    24001 tccttctttt agtcgcgacc aatgcatcaa ccctaaaatt aacccactaa ccagcaacaa
    24061 atacaacaca atacttattc caggaataag atacatttgc caattcgaaa acgtacttaa
    24121 acgtggataa aatactttcc aactaaacca acctaacaga atcaaaaatg gaaacaaaaa
    24181 gaaaccataa tagcaaaacc ataaataacg attgaaatag cgaattcgcc gaccacgata
    24241 cttgaaaaaa tgagctttca attgtcacat tcctcctttc tgtttttact tttcctgtgc
    24301 taaaaacttt tgacaaaaag caactaatac aggaatatca catttagtaa aaaaacataa
    24361 tcgaaaaaga tttaatcgat ttttataagg gtgtgcacac atattacata ttcgtactat
    24421 acgaatccaa atcacttcct gatgatcttg tttccactgg tacggtaatg aaaaacgaac
    24481 aagatcacgt aaaaacatac actgcccaat aaccgcacaa atatatgcat ttgtttgtac
    24541 aaaaatcgtt ggattgtctc caaaatactc aatcaattgt tggatatttt gataacaagc
    24601 acaatctctc aaagtatttg attggtttta tctgaattca aaatctcccc ttccttctat
    24661 cttagataaa aagaatagga agactccttc ctattctttc aaaaaaacct tattttttct
    24721 ctttttcctt agaatcaaca cttggttgtt tttgatctaa acctttttct tgtgattgtt
    24781 tcatattagg attatttgtt ggagcttcta ctttctctaa tcctttcgca taaatcacta
    24841 aatctgaata ttcacgattg ccatcttgtg ctggaatata taccacatga tattctggat
    24901 caattaattt cacaggatca cgtcgtttaa aaccagtcaa atctacatct gctggaaatt
    24961 taactcgtcg tgcaatcttt ggttcatctg gatttccaaa aactaattgt gctcgctgtg
    25021 atcgaatttc tcccgtataa tcgcctgttt cattatcaaa tacacgatca gactcttcta
    25081 atgcgccaaa gcgtacggct cctaaacaag tttcatctaa tgcttctgta atgtcataat
    25141 tatttggaaa ttttaatccc atcttacaaa acctccccaa tttttacacc agttaaaaag
    25201 acaccaacat cttgatagcc agttgttcga cgctctgttt gtcccatatc atttgtttca
    25261 cgactattca ctcgttcttt acgaaaaaca aaataagctt cttcaaatga aatacgcacc
    25321 tctttttctg gtaatcgtaa tcttttatcc tcttcagctg tgagataaac aaaaaaagca
    25381 ggctgttctt ctcgcgaaca ctctgcttgt aattgaacat atcgcccaga cacaccatta
    25441 atttcctttg gtggattagg ttcccgtgaa cccaataaaa atacttgacc taaaaactct
    25501 ttcttcatac gcaatgcttt ttctgccata aagtttttcc tcctcttaaa aaccaaaata
    25561 aattctaaat atttctacat tacaatcaaa caagcggtta aaaatcctaa ccgcttgttc
    25621 ctcatgacta atctttggct ttacgtactt tataaagtgt tatacctaaa gcaatgacta
    25681 atactactaa tccaagaacc ataagtatac tacttgtttg ttcacctgtt tttggcaaca
    25741 ttggtacaac agtagctggt ttaacccgtt gttctggttt cactggttgc ggtggttttg
    25801 gcgcttccac aatttgaacc gtttgatctt tgtttgttag atctacattg tgtttcgcca
    25861 cttcttcaaa cgtttctttc tcatcttccg ttttcgcttg gagccattcc gcaaaactca
    25921 agccttttcc tgcaagattg cgaccgtcca ctaagaattc aaataagaat tccatcattt
    25981 ctttatcggc cgcaaagact ttttccgcat gagtgccttc aaatggtgtg ccgtctggtg
    26041 ttaagtagcc ttgaacaatt aagcgataga tttgaccaat cgcaagaccg tctactttga
    26101 cccattcaag cacagtttga tttggcaagg ccaagatctc ttttccgcca tttttattcg
    26161 tcgctttcgt cgtaatggtt ggattactga ttttgatggt ttggtcattg tcggtaatat
    26221 ctgcgtgaga agccaataat acgccttctc taaacagatc ttcaaagacc acgacttctt
    26281 tgcctcgcaa cgcacgtgcg tctagaataa agtctagatt gactgtacca tccgttgttt
    26341 ctgccacaaa cgttaaggta cttgtgactt cttttccatc tactagcact ggtttttccg
    26401 ttactttgtc cattagaaca ccgttgactg tgtatgtttt tccaacgacc aaatcgtggt
    26461 atttcacagc gtctgtaatg gtgactttcg ttaatggatt aacctctttc aagccagtgg
    26521 gtttatgtgt agccgttgta cgtacacttg ggttagttac tcgtaccgtt tgccctttgt
    26581 cgttgatgtc tttatggttt gctagttctt catcatttct aaaaagactt tcaaacacga
    26641 cgatttcacg accatttata cctgtcaaat caaaggtaaa tggtacgatc accgtttcat
    26701 tttctgcttt ggcaataaag ccaacactcg tttctaccgt atttccatgc acaagcaagg
    26761 gttcaccggt tgctttatcc attaagatcc ctttgaccaa gtatgctttt cctggaagaa
    26821 tctcggtaat ttcaacggta tcgtttaagg taaacaatcc tgttgggtct gcgactgtca
    26881 atccgtcttc aaagacagcc gtggtatgaa cggttggatt attcactttc acactttggg
    26941 cttcatcgtt gatatccgca tgtgctgcaa cttcaatgcc atcacggaaa agtttttcaa
    27001 aaacaacgat cgtttgacct ttcagaacac tagcattaac cacaaattca aggtcttttg
    27061 tgccattgac tttgtctgca ataaaggcca aggtacttgt gatttctttg ccatcttgta
    27121 aaattggcgc attcgttgcc ttgttcatca atactcctga aatggtgtat tcttttccga
    27181 caaacaagtt ggtataagaa acactgtctt taatgacgat cgtttctaat ggatcaacca
    27241 ctttttcacc atcggcttta tttgacgctt gtgtttctag ttttggtgga atcaagtaat
    27301 tacgtacaga aaccactaac gtttccccat catctggcgc attttgtttg aatgcaaccg
    27361 tgaatgcttg tttatctgta tttggttgga aactttctaa tggggcattt tccaagaagt
    27421 aatagtcatc ggcaactaga cctttcacta aaatttcccc gttttgatca gtggtatatt
    27481 caccaattgc ttgattttta ccgttataca aggtaaagtt cacacctgcg agcggcgttt
    27541 tcttcttgtt ccattcttca tacttgaata attttacact tttccgtgct tgacggttcg
    27601 caaaatggat cactttgatc ggttctgtat cattggtaat cgagaacact aatgggtttt
    27661 gattcaaaac gtaatttacg ggggctttga tttcttccaa acgatagcga tcttttccat
    27721 aagtaagtgt accgttagtt aggaaaaagc ctttttcatt ggttttaaag atatccgttg
    27781 tttctgtatc atttggaatc tccattgtga cataacgatt ggctaaagta tcaaaaatct
    27841 tgaattctgt attagcaagt ggaatcactt ttcctgtttc ttgatccact ttcaccactt
    27901 taagacgttg ttcaattact ttatcttcta aaacataatg gaaagtttgc ccattttcag
    27961 aaattgtgac ttcaagtggt tcaaatagtt tatacccttc ttttcctttg gtttcagaaa
    28021 gaatgtacgt atcaaagaca acggtatttt taccaaaagt agcataacca tcattgtctg
    28081 tcacaacggt tttcaccact ttgccagttg tttttgacgt caaagtgatt tcaatgccag
    28141 ctaattttgg tttgttatct ggattcgttg aatcgactaa atctttatta gcaactttca
    28201 ctaaatcaat ggttcctttg atcacttgat cttttgcggt cgcattgaca gtttttccag
    28261 gttcaacaac aatttcaatt gcttcatcag aaagcacata accttttggc gctttgattt
    28321 cttttaaaac atatttgcca ggatcaagat ctttaatttc tgcctgtact ttgccgttaa
    28381 tttttttcaa ggtaatttct ttaatctttt tcccatctgc ttgatacaaa ccatagaccg
    28441 cattttctaa agaagctgca ccttgtgctt tcacaccagt ttccgcatct tctttaaaca
    28501 cacgaatgtt tccttgtttt agaatttgta aattaataac agacgcatta ggttctgttg
    28561 gatgtaacac accaattgtt tgagctcctg gacgacgata ggctaatggc gcaccttgaa
    28621 ctttaatttt attcaattga atttttgctt tatccggtgc ctcttttgtg actgtaatcc
    28681 atacttcatt gccgtgaata gatacattga caccttttgg tttttgaatg tctttcacat
    28741 atttcaaaaa atcattttta ttctttactt tcaacgtttc gccaattttt accgtatgtg
    28801 tttgattatt aaaagttgct ttttcaccaa agttattaat caaacgttga atttctgctt
    28861 ttcgacgatc ataatcaaca cggatatttt tgattgggat ttttgtgccc aaagcttccc
    28921 aaatcataaa ttgtgccgca atgtattgtt catcggattt gtctttattt gtgacatatc
    28981 ctaaataaga gatcaagctt gtttttttcc gtacatttga atttttcaaa tagtttttca
    29041 aagcagtagc tgtataaact tgaccttctg cggctgagac accaggctca atacaaaacg
    29101 ccacttgtcc attaatctca aaacgaacca tataatttaa agtgaagttt agattgttcg
    29161 gactagaagt aacaccaata ttttcgcccc aatcaatttt ccgaccacct aaaacagctg
    29221 cattggttgc acggcgaaca cgaaccgaac gattggttgg tactgttact aacagtgtag
    29281 gtgtacctgt ttttggaatg ttctgctctt ttttcttttg attttcaata ctagccgtag
    29341 attctttgtt tggtttcgtt ggttgatttt gactagctgc ggttgaagag attgtttttt
    29401 caacgttttc ctcatttttt ccttctttgt cagcagtagc cgctggttgt tccactgttt
    29461 cttctattcg tttcttttct tccgcttgtt tctcagaaga ctctggctgc acagaaggcg
    29521 ttgcttcggc agctttttct ccttctagag tggacgtttc tgaagcaggg gttgtaattg
    29581 aggcagtttc ttcaactggt tgtgaagtac caggactatt ccctgggtgt tgtgtttccc
    29641 caatcgtctg atcgagagtc gttttggaat tgaccgttgg cgcttctgtc acaggggcct
    29701 gttcaacagt ggcttcacca gctgggaccc cggcttctgc tggttggact tctgtcactt
    29761 cagaagcgac ttgggcaatg gcttccatcg taggttgtat agccgtcgtt tcttctgcat
    29821 gtgcttgagc agttcccacc aagaaacaga attgagaaaa agccaccaat gcgagtgctt
    29881 tcaaccaatt cattttcgtt tttgcataca caagaattcc tccttttcct gagaatttta
    29941 tataataaaa caaaacgccg ttaccaaaag tgataacgac gtgaactgtc tcttctattt
    30001 ttatctggcg ctcttatctg aacacatttc atcaatgatt ttaccctccc actataatta
    30061 cagaacaaaa aaagaacctg cttcgtggaa atcaactgtc catttcccaa ttggaatacc
    30121 agcttttttg gcaacttcac catcaccaaa tctcatgcca taccaattac taccttcatc
    30181 cttaatttct ttccaaccat atgacatagc ttcatctaat gaatcaaaaa tcatgacact
    30241 attgccaatt gttacaggat ctggcttaag atcttctgga agagaaatat caatcatacc
    30301 ataatgatcc gattcctcag tattattgtc attactagct actggtttgg ttgttctact
    30361 ggcttatttt gatacaacaa gtactcttat tcttctttta cttctccgtt ttcaatttcc
    30421 cattcaaacc actccgcaaa gtcttgctta aaaccatcta ctcgatacct attttgtaaa
    30481 taggcatctt ctgacgtcgt aggatctaaa taggtaggtt tattctctgg acgagcatag
    30541 acacctaccc atgtttcgta atctccttca aaataactat ctaccaaata gccttttttt
    30601 ctaagaatct cccgaacttg atttttaatt attgatactt gttcaaaacc atcactcatt
    30661 ctatcacctc aaataactct agattctcat aaatattccc gacaatttca atcctatcaa
    30721 tgatctctaa caaatcgcta cttatgcttc cccattcgta taagaatttc ccttccgcaa
    30781 aataaatttt ccataaactt cttcatgatc gtcccaacca atatcactct cgaaaaattc
    30841 cacgttgttc ttatctttta gctcagtaaa tgccataagt tcaacatgtt ttccaacctc
    30901 gaaacaatca caccacccat caaccgttac acgtgtgcct tcaataccta agaaactttg
    30961 tcctcgtgta ttaaacacta aaactttccg cattttttgc tcattaatta atcacgcgcg
    31021 aaatctagga atcacaactt attcgcttct tctttataaa taaaaaaact ccaataatcg
    31081 gaaattacta tcttttcctc attccttttt taaatcttca tacttcttct tatctttgtc
    31141 ctaacctaat aacacaaatt ttgaagaaaa actcagtaaa acctgattct ttttttatct
    31201 tcacaacaac caactttttc ttcagatcct tttactagaa cttgtataca aaaattgttg
    31261 aatgctgata taatttgcgg ctacttgaaa aaacgataga tgtaaaaaga taaatagaat
    31321 cattttttat tctatatcag tctatgattc attgcttatc aatcacccaa cccattcgat
    31381 gccgtttttt ttagagaaat ttaaaaaaga ccgtacgaat ctcgcattat ccgattactt
    31441 ttccagaaaa gatccccaat tctgtataaa taaccctatc ttcttgttgg tttcattttc
    31501 tcatataaga attcagtcaa tctacccact atgaccatta ctacgtccaa cacaactgac
    31561 atattaacca cgtaagtatt ccctcacaga tctccgaatc tccatggtat ggattcccat
    31621 cctgacgctg cagaggacaa catatttcat gtggctctgt acccttttga tttggaaaca
    31681 ctacaaatca atactcatct ataacttatc aagcgaactt tcgctaggct tctagtgatt
    31741 actaaattgt tggataacat acatagccca ttttatctag ctgttattca taacagttag
    31801 ttcttgtatt acttcttccg cttgtgttgc cattgagctt gtcaaaaagt tacttagaaa
    31861 aaagctgcca gtaaagcaac tttcaacaaa ccctttttgt ttcttatcat atcaagaaac
    31921 ctccttttat aaattttttc tataataaaa aacgtcgata acttaataag tatcgacgta
    31981 aactattttt atatcccgta aatcaa
//
LOCUS       DAZKNH010000025        30459 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000025 DAZKNH010000000
VERSION     DAZKNH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.025"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACG8MG_001072"
     CDS             complement(451..1761)
                     /locus_tag="ACG8MG_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFQ0375136.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3131)
                     /locus_tag="ACG8MG_001073"
     CDS             complement(1773..3131)
                     /locus_tag="ACG8MG_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0375137.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
     gene            complement(3333..4901)
                     /locus_tag="ACG8MG_001074"
     CDS             complement(3333..4901)
                     /locus_tag="ACG8MG_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFQ0375138.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4905..5315)
                     /locus_tag="ACG8MG_001075"
     CDS             complement(4905..5315)
                     /locus_tag="ACG8MG_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0375139.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5312..6235)
                     /locus_tag="ACG8MG_001076"
     CDS             complement(5312..6235)
                     /locus_tag="ACG8MG_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFQ0375140.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6232..7110)
                     /locus_tag="ACG8MG_001077"
     CDS             complement(6232..7110)
                     /locus_tag="ACG8MG_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFQ0375141.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7097..8434)
                     /locus_tag="ACG8MG_001078"
     CDS             complement(7097..8434)
                     /locus_tag="ACG8MG_001078"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFQ0375142.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8437..9540)
                     /locus_tag="ACG8MG_001079"
     CDS             complement(8437..9540)
                     /locus_tag="ACG8MG_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0375143.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9614..11122)
                     /locus_tag="ACG8MG_001080"
     CDS             complement(9614..11122)
                     /locus_tag="ACG8MG_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0375144.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11088..11780)
                     /locus_tag="ACG8MG_001081"
     CDS             complement(11088..11780)
                     /locus_tag="ACG8MG_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0375145.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11802..12776)
                     /locus_tag="ACG8MG_001082"
     CDS             complement(11802..12776)
                     /locus_tag="ACG8MG_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0375146.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13076..13948
                     /locus_tag="ACG8MG_001083"
     CDS             13076..13948
                     /locus_tag="ACG8MG_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0375147.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14406..16604)
                     /locus_tag="ACG8MG_001084"
     CDS             complement(14406..16604)
                     /locus_tag="ACG8MG_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0375148.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16836..17828
                     /locus_tag="ACG8MG_001085"
     CDS             16836..17828
                     /locus_tag="ACG8MG_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0375149.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17888..19309
                     /locus_tag="ACG8MG_001086"
     CDS             17888..19309
                     /locus_tag="ACG8MG_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFQ0375150.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19302..20801
                     /locus_tag="ACG8MG_001087"
     CDS             19302..20801
                     /locus_tag="ACG8MG_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFQ0375151.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20840..22234
                     /gene="uxaC"
                     /locus_tag="ACG8MG_001088"
     CDS             20840..22234
                     /gene="uxaC"
                     /locus_tag="ACG8MG_001088"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0375152.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22393..23076
                     /locus_tag="ACG8MG_001089"
     CDS             22393..23076
                     /locus_tag="ACG8MG_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFQ0375153.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23078..23914
                     /locus_tag="ACG8MG_001090"
     CDS             23078..23914
                     /locus_tag="ACG8MG_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0375154.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23925..25679
                     /locus_tag="ACG8MG_001091"
     CDS             23925..25679
                     /locus_tag="ACG8MG_001091"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375155.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25955..26296)
                     /locus_tag="ACG8MG_001092"
     CDS             complement(25955..26296)
                     /locus_tag="ACG8MG_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0375156.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26364..28535)
                     /locus_tag="ACG8MG_001093"
     CDS             complement(26364..28535)
                     /locus_tag="ACG8MG_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0375157.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28746..29603
                     /locus_tag="ACG8MG_001094"
     CDS             28746..29603
                     /locus_tag="ACG8MG_001094"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0375158.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29674..>30459)
                     /locus_tag="ACG8MG_001095"
     CDS             complement(29674..>30459)
                     /locus_tag="ACG8MG_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0375159.1"
                     /translation="KLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDN
                     YGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLT
                     VGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIE
                     ARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQ
                     QFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         9099 a         6274 c         5391 g         9695 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct tgtttttata ttcacaatgt tggcttttta
     3181 ttgcattatc ttgacagata aaaaacaaaa aataatctaa aaacattgat gcataagatt
     3241 gataactttt tttacataac aatatataca aacaaaaaaa ttcactaact agtgaaaata
     3301 agttagtgaa cctcatttat taaagaatta aattacagat gatcttttcc atcatacttg
     3361 aaaaactcaa aatcagcata acttcctctt tttttattta gatcatgcac tgctatcccg
     3421 ataaaatttc cagtaaaccc tcctgagagt atacacatgt ttttttgcgg acctactttc
     3481 ttccattctt cagttgatac gtctttcaaa tgaaactgtg ctgtctcttt ctttacagtg
     3541 actttcaaaa aagtcttctt ttctgtgagt ggaatcgtcc aatcttctat ttgtgctact
     3601 ccgttttctg attctatcat acgtaggcat tttcccttgt cctcattcca agtaacataa
     3661 caataaatat aattggattc atttaagtac agtaccagtc cagccatctg attaaaatta
     3721 ttaggcatgt attcaaatgc tgtctccgca tcaaaagtaa aatccgtttg ccgaatagct
     3781 aacaaatgat gttcgaaggt agattgaaga gattcaccgg aaatcagacg taaaaaacct
     3841 ggtctacatt ttgtatcaca ccaatcagca tttggcaaaa tgcgtctggc attccagttc
     3901 ttctttagct ctccttcaaa tgtatccata aactgatgat ctttttcttg ttgtacctct
     3961 tcatttgtct caatcattat ttcttccatc ggagttgtct ggctatcttt aagtcttaac
     4021 caatcgtcgg acgtccaata cacttcttga atagcggttt ctcttcccaa aatagccgca
     4081 ccgtttaatg gtctggtaca aagatatgtc atgtaccatc ttcctgacgg ggtttgtact
     4141 aagctcccat gtcctgtaca ttgcaatgta gaagcaggtt tgtcactagc tgtcagcata
     4201 ggaaaaccag gatcaagctc gtaaggacca gtaatattct ttgctcggca aactgtaact
     4261 gaatgtcctc ttcctgtacc gccttctgcc gtcatcaaat aataataccc gttacgaaaa
     4321 tacaaatgtg gtgcctccgt tttcgctaat tctgtacctg caaatatttt atagatggga
     4381 ccatcaagct tcttcttctc tgcatcatat tcttgtaata cgataccgct ggatttattc
     4441 ggtgtgtcca gacgataatc ccatagttca ttgacaagcc atttttttcc cgtttcaaga
     4501 tcatggaaca aagaaggatc aaagccgtta ctatttaaat aaattggttc ggaccaagga
     4561 cctgtgatct cagatgcagt gatcaaataa ttgtggacat ctttaaacgg acggcctgca
     4621 cttttgacat ctgtgtaaac aagataaaac aatccatcag cataactgat ctgtggcgcc
     4681 catatacttc catttgtagg atttccttgc aaagatactt gagtggtcaa aatatccgtt
     4741 gtatgtgacc agttgactaa atttttcgac tcatatactc ggattcctgg aagccattca
     4801 aaagaggaga caataatgta ataccgctct ttcactctta atatattagg atctggattg
     4861 aatcctctta aaataggatt tttcactttg atagtctgca tgattcaggc tccttttctc
     4921 attcttactc gatataaact gaatgcccca gctgcgaaat aaaaaaatgc aaaaggaaac
     4981 gcaataaact gagtgccaat caacaatatg aatcctgtta taaaatacaa accagtggca
     5041 gttgttaagt attttccttt agcagtcaaa aacaatgcta ctgctaaaaa agcaagtgaa
     5101 ataaacaaca caacggcaaa cagaccagtc atgtcctgta ataatccaaa tgtattttct
     5161 aaagacaaag ttgaatgatc tacagcaagc gactgataac tttttttaaa ctctgacgca
     5221 gacatattac ttaaagtaac tgctaacccg ccaaagaaaa tcaaagatag actgccaccg
     5281 accattccta gcaatagttc ccattttctt ttcatttctg aaccccctgc ttttctaaat
     5341 gtatccattc aggtcttttg atcgatgttt cataattgtt attacgttca gagaatttga
     5401 cagtctgttc atttttcttg tcatcccaat tatcccaacc agttgggtga acatgcccac
     5461 ctatcttaca cgcttcaaat aagacatttg catacggacg ccacggtctt cctaaataga
     5521 cgtttctagt atcaggttct gcagtcaagg tacagctttc aaaacgatac cctgtttttt
     5581 gatttttcgg cgtagatgct gctgtgatgt atccaggatt tttactggct aagctatgga
     5641 tatgacactg atgaaaaacg gcatctgccc caccaaatat gaaatccacc gttccttcaa
     5701 tccagcaata ttcataggtc tgacgacaat actcaggatt ggcagaagcc ggtgtacaaa
     5761 aaggagtacc atctttttgc aaatcaggca acggacccgt gcataaggta tcctgataac
     5821 cagatagtcg acagtttttt aaagatacgc gatctcctga attgaataaa gcaacagcct
     5881 gtcctacctt ttctcctgga cctgctgtat tttcgatgtg aagattttct aatacgacat
     5941 tcgatccttc taagaaaaaa gtagcacttc ggaaagtccc taactggcta ccgtcaagat
     6001 actcctgttt tgcaaaacga ttaccggaga taactactgt tcctgctccc acaagtttaa
     6061 aattggataa tctgctttct atatattcat catatcttcc ttcttttact atcaagacct
     6121 ttggatggct atccggcagg cctgccaaat aatcgattcc ttcttggata gtttcaaaat
     6181 ccgtcggggt atctatagca ttctttccaa ttatcagttt gtctttcatc ctcataaatg
     6241 ctcccttatg aattgtttcc aaatatttct catttcttgt gtttccatat gaccgcctgt
     6301 taaagaaaaa gaagaaaagt tttctggact acccattgat tgatactttt tgtccagccc
     6361 ttttctcagc ttcagtacgc cttcgtccaa gcacatcgta tcgttttttc caactaagct
     6421 aagacgaggg cgcggagcga ttttttcttg gattgctaac gttgtataag ctgtcaatag
     6481 atttggcaca tagtaataaa acccatgatg atctaggcgg cgatgtagca aaagtatttc
     6541 gatgtccacc tgtgccgcta gatcaataca aactttgaca cgagaatcca atgctgtcac
     6601 ccaccagctt aataagcctc ccatagaaag accaatcgtt gcgagccgtt gagtatctgt
     6661 gtcttccctt gtctccaaat aatcgatcaa gctgatcaca tcataaatac gcattcccca
     6721 taacgttttt cctgttaata aaaattgttt gaacaactcg ctttcagaaa tacctccgcg
     6781 ttcttcaaat ccccaagcat caatcgacca aacagcatat cccattcctg tcagtacttc
     6841 tgcaaaagat ggagaagcta agtaaggagc accatggatc aattcacttt tcccgactga
     6901 aaaatctccg ccgtgagaat gcaggtaaag gaccggcgga aacggtcctt ctttttctgg
     6961 ataagcatag agggctggta ctgtttccaa ttcatttaag tacaattgat agtattcaag
     7021 cataaaaccg ttttgttttt ctcggtataa tcgttttcca ctaggggtat gtctgctaga
     7081 tggagcacct aaaaaatcat tcaactgcat aagcaaagtt tttacgaaca accagttcat
     7141 ccatattttc tgattgattt gattggaatt tctcaatact gatttcttta gtattttcta
     7201 atagaaatgc tggtccctcg tgattttcaa tcgacatctg gttaaaggaa accttacccg
     7261 caaagccaat ataaaatcct tgtctgctca tttcctctat tcctgtcatc atagcaggaa
     7321 caccgggaac tgcatcttgg gccatcgaga catcgatatt gtcaaatgtg atttcttgta
     7381 tgaattgttc cgctaatcca taaataaaac ctgcagaagc atggacattc cttgccgtga
     7441 tattcgaaaa atggatcctt ctgaatgctg gtgtccgttc atctatcgga taagcctttt
     7501 tttcccatac atacggttct ttccctcgcg gaccacagaa ataataaaga ttcagtataa
     7561 atggacacat cacattatcc atgactatat tggatactcg aatatcttca acgatcccgc
     7621 ctcgacctcg tctagatttc aatcgtattc ctcgatcagt ttcttggaag atacaatttg
     7681 aaatggtaat attacgaata ctaccactca tttcacttcc taatacgaca ccgccatgtc
     7741 catggaccat cgtgcaattt gtgatcgtga tattttcgca agcgatcctt tcgtacgtat
     7801 cttcagtccc tgccttgatt gcaatacagt catctcccac gtcaatatgg caattactaa
     7861 tacgcacatt tttgcatgat tctggatcaa ttccgtctgt atttggtgaa tccgctgggt
     7921 tcaagatcgt caagttatca aacgttgcat tactgcaaag aatcgggttg atcgtccaac
     7981 ttggtgactg gatcaattta atatctttca cagtaattcg atgacagtta tggaaactca
     8041 tcaatttagg acgaggataa gcgagattat ctggttcatt tctaaaagta tgccaccatt
     8101 tttttccatt tccatctaat gtaccgaaac cagtgacaga gatgttctct acattttgag
     8161 catagataca ggaagcataa acttttcgat ggactccttc ccaacgtgaa tgaacaacag
     8221 gataatcttt tggatcgtca gagaatttca gaaccgctcc tgcgcttaaa tgaagttcga
     8281 cattgctttt taaaaataaa gctcctgtta gaaattcacc tgctggaaca actacggtcc
     8341 cgcctccgtc acttgctgca gcatcaatgg cttgttgtat tgcttcagta ttcaattcat
     8401 caattgatgc gccaaatgtc aaaatatcat acatatttat aatgcctcca cttcaatcaa
     8461 tgcttgcaag aatgcaccaa cgcctttttg atcattacaa ataattggtt cactgatgta
     8521 gtaagcatat gaaccatctc gctgatctgc tccgcctaac cctgctactt gacagttttt
     8581 attcaagttc agccagcctt cttttgtttc cagaacaaat tcatcaagta atccttgatg
     8641 tgatttcttc acttgtggaa gccattcttc tttgttcagc agtcccaaac gtattccttt
     8701 agccattgcg taaacaaaca tactactgcc ggaagcttcc agataatttc ctttccgttt
     8761 tgtttctgtt gttacttgat accatgtccc gctgttttct tcttgatatg gaagtaatgc
     8821 agccagtgtc ttttctagaa tttctttcag caaagaacaa tctgtttctt catgaagtaa
     8881 ttcataggta tctgctgcag ccatcaaata ccagccaatc gatcggctcc aaaaatgatg
     8941 ggatagacct gtttttggat cagcccatgg ttggaccttc ttttcatccc atgcatgaat
     9001 cagcaaattt gtttttggat cgaatagatg tttataactg atttcaaact gtctcaaaac
     9061 atcttggtaa tctttttctt cactaaaatt ctggataaat tcagcataaa aaggagaacc
     9121 catatataag ccatccaacc aaatttgata aggataaatt tctttatgcc aaaaagctcc
     9181 ttcggatgtg cgtggatggt ttgccaactg ctgggaaagg agttctgctg cttttttata
     9241 tttttcttcg cctgtttctt tgtacaataa aaagaaaagc tttcctgtat ttacatggtc
     9301 gatattgtat tcttcaacgc tgtagccacg gatcgttcca tcttcttgca cgaaatagtc
     9361 gatattgtct tttatgaaat caaaataaat tttttcttgg gttcgttccc aaagtaattg
     9421 gaacccttta agtacgacgc catagtcata agaccatttt cctttatacc acttttcttc
     9481 atataagcga ggtgttcttt ccatgatact ttttgctaat ttctttgacc agttttccat
     9541 tatttgctct cctttaaaaa ttaaaatagg atttgggaca aataccaaga agtatttgtc
     9601 ccaatcgtct atattactgt gccgctgctt tgtatgcttc ctcgtactct tgtgtgattc
     9661 gatcgcctcc ttgtgctttc cagtcttcta cagctttttt gaatccatct tcgtcaagct
     9721 gtcccatgat gtattgcacc gtagcatcaa tgacgatttt ttcaatttct gatcctactt
     9781 cattgttcgt cggtgaatcc aatggcacag aaggatccat tacagcaaat tcttcatttt
     9841 ctttgatcaa ttgattagag aattcttttt cagggtctgt atctttgaaa gagaacgtca
     9901 cttcactagg acgagagctt gataacggtt gcacctcttg ttgccaaaga tccatatcga
     9961 taaattcgat agaaccatca tcattcaatt cataatgcgt tccttcgata ccgccagtca
    10021 tcaatttata cacatcttct tctaaaagat catcaacaaa ttgaagtaat ttcttcaatt
    10081 gtgcttcgtc ttttacttga gatttgggaa atgctagcaa gccgccaata ccatttcctt
    10141 ctgaccaaac atgataaccg ccgccttcac catctgagat cttgtttaca ggcactaatt
    10201 ccatatcatc ttgaatacct tgacccatcg tcttcagatt cttgatatcc accattccag
    10261 tgtagattcc agttcttcct tgtgcaaatt gttcttgctg atcagtcttt tgggtaactg
    10321 caaagtcttg tgccaaatag ccattctcaa atagatctct tgaaaaattc aaagctttca
    10381 tatactcttc tgtatcaaat tctggagtga atttgccatt atcgtctaca gcccagccat
    10441 ttggtgctcc aaaataagaa gtgaaaagtt taaagctggt ataacgaata tcactaggcg
    10501 cacgatcgcc aaaaccaact gtgtcgtcca caccatttcc atcaggatcg ttttctgtaa
    10561 attgtttagc tacttcataa agctcatcca tggtagttgg aacatctagt cctagattat
    10621 ccaaccagtc ttttcgaatg actaagccag cacgagcgta gtctttttgt atcggtacgc
    10681 cgtacaaggc accgtcgata ctcgcagctt cgattctatc ttcagaaatt tgacttaaat
    10741 ttttatagtc ttttaaatat tctgatacat cccagaatac gcctgatttt aacgcattgc
    10801 ggacagaaga gtttttcaac atcgcaattt gaagtgtaac gatatctcct agttcatcag
    10861 aagccaatgc agttgttaat ctttcttctt ttgaagcatc aggaatccag ttgaatgtca
    10921 gttccgtatc ggttttttct tcgatcaagt tcacaatcgt gtctgtaggc ggtgaagcat
    10981 gatgcaaaat atttaaccaa gtgattttag cttttccatc ttctgtctct gaagaactgt
    11041 tacttgaact gctgccgcat gcggcaagtg ctagaattcc tgcaaaacta agacctaatt
    11101 ttacgagcgt tgcttttttc ataaattttc tccttcactg ttttaataat atttttgttt
    11161 tcttctgttt tttctgtata tgagttcttc cattccaaat ggcaaaatat agaatttgct
    11221 gtccacgtga ctgcggaaaa gaagagactg ccaccgataa aggttaaaac acctggcatt
    11281 gtttggatga tcatcagata aatccctacc atacttaata tcaacactaa tgagtaatga
    11341 agatgtgaga atccgaataa aaatgccatt ttcattttga aaaaatatcc tttccaatca
    11401 taatgagcca tgattgggaa aatatagagt gctgagagca catatatgaa cgcagcaata
    11461 tacaaagcga tcctaagata ccaattgtgt atgaaagaaa gatccacaag taaaacaatc
    11521 ccgatggcgc catatatcca gctcatcagc aaggtttcac gatagttttc tttaaaatat
    11581 gtgaagtacg ttttcgtcaa aggaaacgtt tcttttgagc gcagccattt tctaatcact
    11641 gcaaccatcg catacgatgc aggaccgaaa gtaaaaactc ccaaacctgc gatgactaaa
    11701 ccgccccaca gaaaattcaa ataaaccaac tgtaaaatac gatttagcct tccgtttatt
    11761 ttatctaatg actgcagcat atctttccct ctctttctat cttagtaaac gccgtcttct
    11821 cctagctttt tggcaatctt attagctaaa attaccataa acaatcctac gaccccttta
    11881 aacaatccca ttgctgtact gaaacttaac tgtccattct tcaatccagc tgtataaata
    11941 taggtgtcga aaatttctgc aacgcttcga ttcaatgaat tcaataacaa gtacatatgc
    12001 tcgaacccta agtctaatgt ctgaccgatt tttagaatca gcaaggtaat gattactgga
    12061 cgaattgccg gcaatgtaac atgccatgtt ttgcgtaagc gtcctgctcc atccatttct
    12121 gcagcttcat acaactgtgt atcgactgca gttatcgctg aaaggtagat gatcgtagac
    12181 cagcccaact ctttccagat cacttgtccg atatatactg tgcgtgtcca ttcaggggat
    12241 gttagaaaac tgattttttc aaatcccatt gttgcaagga attcattcaa tacccctccg
    12301 tcaacattca aaaatacata agtaatcgaa acaatgatta cccaagacat gaagtgaggg
    12361 atgtaaatga tcgtttgaat cccgtttttc aatcgtttgt tcgttacttc atttaatagt
    12421 aaagacaaaa tgattggcat cggaaaaaac acgaagatat ttaatccgaa caagatcaac
    12481 gtatttctta ataatgtaaa gaatgtcggt tctgtaaaca aacgaatgaa atgtttgaaa
    12541 ccgacccaag gacttcccat tattcctaaa aatggttgat aatcttgaaa agcaataacg
    12601 agtcccccca taggaagata cctaaaaatc aagaaataaa ggactcccgg cagaatcatc
    12661 acatataaaa atttattcgt tttgattcct gcaattattt tttttcgtct tctgagcttt
    12721 tcttcttttt tcgatagcgt cagttgaaat ggttcttgtt tctttacact ttccataagc
    12781 ccctccttag ttttgtgaat gtttgctgct tatgacttca tgatagataa ctatctgaat
    12841 tacgtatata atcattttta ccgtaagtaa aaacgcttac atagcaatga tttttgtgct
    12901 atacaaaacg ataaagtgta ttttttcaca aataaactac gcatagcaaa ccttctattt
    12961 ttttctatct gacacttagc ataggaaatg attatatacg taatctttca ccgatgataa
    13021 attgaacata tagaaacaga aacatttaat ttattttgag gaggagccta tctatatgaa
    13081 aaagaaagtc tctataggag aaagaagttt tacacttttc aacaatacgt ttctaatttt
    13141 gctagctttg atctgtatcg ttcctttttt aaatatcatc gctacatctt ttgcttctac
    13201 acaagaagtc gtcgcgaaaa agtttatcct ttttcctact accttttctt tagacgcgta
    13261 tcgctacatt ctttctacgc cgaccatttt tagagcactt gctgtttcga ttggtgttac
    13321 aggtgtcgga acgatcgtca gtatgtgtgc aacttcactg atggcctacg gtctttccag
    13381 aaagtatttg tttggtcgag gtttcgtcaa ctttcttgtt gtattttcta tgttgttcag
    13441 cggggggatg attccaacgt tcttagttgt tcgttctcta gggctggtca attcttactg
    13501 ggccatgatc ctgcctgtgg ctgttaatgc aatgaatatg atcattatga gaaacttttt
    13561 ccaagccttg ccggatagtt tggaagaatc agcaaaaatg gatggttgca cagattttgg
    13621 tgtatttttc aaaattatgc tgccattagc gttaccgtct attgctacta tctcgctttt
    13681 ttatgctgtt acttactgga acacttatat gactgctatt ttgtatatca acgattcatc
    13741 taaatggccg atacagattc ttttacgcca aatcgtcatt gtatcaagtg ggatgcaagc
    13801 agaaagtagt gccgttgatg tgattccacc agcacaaaca gttaaaatgg ctgttatcgt
    13861 tattgcaaca gtgcctatgc tgattgttta tccttttgtt cagaaatatt ttgtcaaagg
    13921 agcactcgta ggttctgtca aagggtgatt atgtactgaa cgcaaaatgg tagttgtgtc
    13981 agaagtggtc agcttcaaga aataagaagg catttacgaa aattgcttct taaatttttg
    14041 tgaaatttcg gcttattttt cgaagaatct actcctggaa caccgtgtat cagaggttgt
    14101 gaaagagacg ttcagccttg agcgatgata aaaactctca caaacattgc gacgagtaac
    14161 ccacaggagc aataagaagc gattttgaaa aatagcttcc catatttcac aaaattatgc
    14221 cttattgtcg atagcctgtc tcttgaacgc catttattcg taaaaaagaa ctgtgacaag
    14281 acttttgtca cagttctttt tgcgtgctta gccttgaaat ttaactatcc ataaatacaa
    14341 atactagggt tattctaact tcttttattg cttgaatcag tccacaagaa aaacagcctc
    14401 ttgattcaca aatatttttt tcgatattct cccggcgtaa tcgattcctt ctttttaaag
    14461 aaacgaataa aattttgcgg atttcgatat tgcaatcttt ctgcaatctc ttttacggag
    14521 aggttagtct ctttcaacca tttttttgct acctctaaac gatgattttg aacaaaatca
    14581 ccaaattttt ctcctgtttc ctttttaaat acattactta aatagttagg gttataatgc
    14641 aacctatcag cgatcacatc taaagacaga tcacgatcat attctgtgta gacgatatgc
    14701 ataatattct ccgacaaatt tttaaattca tgatcagtga ttgcttgaga gcattctgtg
    14761 attggcagaa ctaattgttc taaaacaagt ttttctagtt ctttgggatg ataattgttc
    14821 agtgctttta cataaatttg tttcatgtta tcaaagtttt ttgaatctac tcccaataac
    14881 tgtcctaatt gaacgagttc attgatcaag cggaccgaaa ctacttcccg actcaaagga
    14941 tttttatttt gtgcaaacaa tgcatcaatc aattcatgta ctaaattctt cacttcctga
    15001 tttccagaac gaatggcttc aaacaatcga ttttgttctt ctatcggata tctaataagt
    15061 gttttttcat gagcagcagg aacgatttcc tgataaaaaa taattgaact tggaccagta
    15121 tttacacgat aatacagggc ttcttttgcc cgatcaaccg attgcttgct ttcattcagc
    15181 gtttcgaacc ggttactgat gcctatactg atagttaaat tcaagtattc ttttacacgt
    15241 ttttgaatga gtcggcaata ctccatgact tttttgcttg cttctgcatc atctttatcc
    15301 attcgataaa tagtggcttg catttcttca ttcaaaacga tcggcagcat tcgattttcc
    15361 tttggcacaa tttccgtgat catgttgttg atcgctaaaa gtagcaaatc acgctcccca
    15421 ccgtgactat cttctaacag atcgatttga accaaagctg tataatagca gtcattttga
    15481 gaactataac caaattgctg caaccgctga tctagttctc gcctttctac tcgatttcta
    15541 aataaactaa gtacaaatag cgtttccagc tgtggctttt gggtaactaa gtgtgcactt
    15601 aacgcttctt tttctcctat gatccgactg actgattgag cgaccaaatc tagttctttc
    15661 ccactgaatc cttttgattt aagattcaga cgttcctgta tttgagaaat cggacgggca
    15721 aatctgtccg aaaataagta ggaaagaaga ccaatgagtc ctaccagtgt taaagacact
    15781 gcaactagcc cgattctcag attgcggatc attgcgccaa ttgcactaga gtcaacttct
    15841 aatctgtaaa tccaattatt ataatctgat ttcagtacta tatacgtttt atctttttct
    15901 cttatcactt tcagctgatt gtaatctaga tccacgtccg ttaatacagt tggttgctct
    15961 ttatttttta ctgtctgagt agtaaaaagg ttgccaactt cgttttcaat agaaagtatg
    16021 tcttctccac tttcaccaac aacttgttct attgtatgtt tctctatcgt agcaattccc
    16081 aatgcatatt tttctttttc aaacattggc aaagaaatca ccatttcgat tcctttgttt
    16141 ttcttttgcc aaaataaatt atttttgtta tttatgtact tctgttgata cttttcaatt
    16201 tcctcttcgg tcagttgact caacgatcca ttgattactc cccatttacc agccaaactg
    16261 actaaatcat aattattatc atccataaca atcgtttcta taaaatttag ttctttcctg
    16321 atttcagaat aagtggagta atctgtatat gtcagcggat gcttgaatat gtccgagaaa
    16381 tttttggtcg aaccatacgt agaaaacgaa tattcaatcg ttctaatttt ccattcaacg
    16441 ttagttttaa cttgcctgag caaattcttt ttgtctgtta aataactcga ttcgatcgat
    16501 tcataggtat tctggtaaat gaataaacta aatcctatga caagtacggc tcctaataaa
    16561 aacatcggta aaaatatttt ataaaatact gtacttttct tcattttctt cctctcctcc
    16621 ttttaaaata aaaggctttc atttttctgt aaaattgata ctcatctaat taaatgagca
    16681 ttccccgctt tcaagaatag cataattaaa aataaaaatc caagtaattt ttaacgtgtg
    16741 caatcttttc atatcaaaga tctattgact tatcgtagtt atcgtaatat gatgaatgcg
    16801 ttaacaaaaa gtgaaaaagt aaggaaaatg atggaatggt aacgatcaaa gatgtcgcta
    16861 aagcagctgg cgtttctcat acgacagttt ctcgtgcttt gaataacagc agtttaataa
    16921 aaaatgaaac aaaacaaaaa atacgacaac tagctaagga aatgaattat tcgccaaact
    16981 atagtgcaaa aggtttagtg aatcaaaaaa aatatctaat cggactattt ttctcaagtt
    17041 tgcataaagg aacttcatca aatttcctag cagattccat tgcaggcatc cattcagtat
    17101 tagacacaaa ttacagttta tctgttgaaa gtatcgatgt tgttgattta acagaaatca
    17161 acctgcaacg ctacgatgga ctgatcgtta tgagtcaaag tgatgatgat cagcctttca
    17221 tcgactatct caaagaaaaa gaatttcctt tcgttgttgt caatcgtcac atcaatgatc
    17281 cagcggttat caacgtcact gccgatgatg cggccggtgt tatgcaggca gtagattacg
    17341 gtattggatg cggacatacg aagatcgctt atattggcgg aaagaaaaat tttcgttcga
    17401 caaaagaacg ggaaaaaggg gtattgaaaa gtctaaaaaa agcgagcatc cctgtcaatc
    17461 cagcctattt tttaacagga gattacagct tagaaagcgg cctccaaaac atggaatacc
    17521 tattatcact ttctgacctg cctactctgg ttttttgcgg caacgacgac atagcgattg
    17581 gtgcattgag agctgtacat ttagctggcc tccgtgttcc ccagaacatt tctctgatcg
    17641 ggttcgatga ttctccagtc gtctcctatc tcaatccgcc gcttaccacc gtacgtaagc
    17701 ctttgaaaga aatgtgtcaa gaaggaacaa agcttttact agatttgatc gaaggcagga
    17761 cgatcgaaca atctaaaatt gagctctcca ccttgttgat ccttcgtgag tcgatcaatt
    17821 acttgtaaac tacaagtaat tttatataaa aatgttaacg tgtgcataac acagaaagga
    17881 agtttatatg aaaaaactga caaaagaatt agtgaaaaaa aacgaagcac ctgtaaaagt
    17941 tcttcagttt ggtgaaggca attttatgcg tggatttatt gattggcaga ttcagcagct
    18001 gaataaccaa caattgttta aaggtaatgt tgcaatcatt caacctttag aacaaggtct
    18061 tggaaatatg atgaaccagc aagataattt atatacggtc attttacaag gactcagcaa
    18121 tggcgaaatc gttgatactt ccgagattat caccgtagta gaacaaatga tcaatcctta
    18181 tgatgaatgg gatgcttatc tatctctagc agaaaatgac gacttggcct atatcgtctc
    18241 caatacaaca gaggcaggaa tccaattcgt ggagactgat aatttaacag atactccccc
    18301 acagagcctc ccgggcaaac tgacagcctt tttataccga cgtttcaaat taaacaaagc
    18361 tggctttacc atcattcctt gcgaactgat tgacagaaac ggcgaacgtc taaaagaaat
    18421 cattttacgc tacgcagaaa aatgggattt agaacctgct tttgttgagt ggttagaaaa
    18481 agaaaatgtt ttctgttgta gtttagttga tcgcatcgtc ccaggttatc ctcgcgaaga
    18541 tgcaggtgtt ttcaatcaac ggcataacta tgaagatcaa ttgatggtca aagcagaacc
    18601 attcatgctt tgggtgatcg aagggcctaa agaattggag aatcaactac cattcaaaca
    18661 ggctggcttg aatgtcatca tcacagatga tatgactcct tatcgtgaac gtaaggtgca
    18721 tttgcttaat ggaccgcata cagcgatggt ccctcttgct ttgttggcgg aattggaaac
    18781 tgtagaagaa gtaatgaaag atcctttatt ctccgcatat gttgatcagt tattcaatct
    18841 agaattgatt cctatgcttt ctttacctaa agatgaatta gccatttatg ctgaccaaat
    18901 caaagaacgt ttcttgaatc cttttgccca tcacaaatta gaagcgattt cattaaatag
    18961 tgtttcaaag ttcagcaccc gtctgcttcc agtgttcaag aagtatatcg aagaacaaaa
    19021 tcaggtacca ccgttaatca ctgtttcact agctgcgcta cttcttatgt atcgaggcga
    19081 tcaagtaaag ccacatgatg atgaaaagac gataagcgaa tttactgatg cttggtctga
    19141 taatggaaca gctattcctc gattattgca aaacgctgcg ctttggggag aagatttgtc
    19201 tcaaataccg aatgtgacag atacagttca agaaatagct gcaaagatcg atcaaaaagg
    19261 tgtccggtca gtattacaag aaatgaaagg aacgatggtc catgcttgaa accctgcaac
    19321 caacaatgaa attaaatcga cgagacagtg tggtcgtcgc attaacccct attccaaaac
    19381 aaacacaact gacgattgat cagcaaacga tcaccactct tgaagatatc ccgcaaggtc
    19441 ataagattgc attgggtgat ttgaaagctg gagacaacgt gatcaaatat ggctatccaa
    19501 tcggtcatgt gacgacagac gtaactgccg gtcaatggct gcacactcat aatgtaaaaa
    19561 ccaatctttc tggtgaattg gattatcgat atgaaaaaga tgtccatcca agtcattacc
    19621 tttttgagaa ccgaacattt caaggttact tgcgaaaaaa tggaaaagta gggattcgta
    19681 atgatttgtt tttagtacct accgttgggt gtgtgaatgg cattgctgaa ctgatcgtca
    19741 aacagttcaa ggaaaaacat ccagatctgg gacagtttga ccatattacg attttaaagc
    19801 atccttatgg ctgttcgcag cttggaaaag atcatcaaaa tactcgagaa attttagcgg
    19861 acgcagtaaa tcatcccaat gccggaggag ttttagtatt tggattaggt tgtgagaaca
    19921 atacagttcc tgaattcaaa aaaattttag gtgaatatga tgaagaacga gtgaaatttc
    19981 tagtggctca agatgtttac gatgagatcg aacaaggtgt ggctctatta gaagagcttt
    20041 taacagcggc ggaaaatgac cagcgcatat cagtcccgct ctctaagttg aatgtcggtt
    20101 tgaaatgcgg cggttctgat ggtctatcgg gaataactgc taatccttta cttggtgcat
    20161 tttctgacta cttgattgct caaggaggca gcacgatctt aacagaagtg ccagaaatgt
    20221 ttggcgcaga acaagtattg atggcacgag cggaaaatga agaagttttt gactcaatcg
    20281 ttgacttgat caatgacttc aaacagtatt tcttgtctta cggggaacca gtttacgaaa
    20341 acccatctcc tggtaataaa gcaggtggga tcactacatt agaagataag tctttaggct
    20401 gcacgcaaaa atcaggaagc tctcctgtcg ttgatgtgct acaatatggc gaaaaaatca
    20461 gaaaaccagg gcttagttta ttgcaagctc ccgggaatga tcttgtagct gcctctgctc
    20521 ttgcttcatc cgattgccag ttggtcttgt ttaccacagg aagagggact ccgtttggct
    20581 catatgtccc aacggtcaag gtatctacga atacaactct ttttgaacgc aaaggtcatt
    20641 ggatggattt taatgcaggc gtcttattag aacaaccaat ggaagtaatc ttagaatcgt
    20701 ttgttcaaaa aatcatcgct gtagctagtg gagaagaaac gaaaaatgaa caaaatgacg
    20761 ttcgagaaat tgccattttt aaaaacggag taacactttg attttcataa aacagtataa
    20821 aaagaaagaa ggaagattta tgtttttatc tgatgatttt ttattaaaag acgattgggc
    20881 gaaaaagctt taccattcct acgcaaaaaa catgcccatt attgattatc actgtcactt
    20941 agatccaaaa gaaatttacg aaaacaacaa tttcagcaat ctgacagaag cttggttagg
    21001 aggcgatcat tacaaatggc gcttgatgcg agcttgcggc gtgcctgaag aaaaaatcac
    21061 aggaaatgct tctgattttg aaaaattttt agcttggtgt caaaccgtac caaaaattat
    21121 cggaaatccg ctttacagtt ggtcacatct tgaattgaag cgtttcttcg gtattgactt
    21181 gttgataaat gaagaaaatg cagagaaaat ttggacacaa gcaaatgaaa ttttggcagc
    21241 tcctgatttt cgccgtcggg aaatcgcaaa aaaatcgaat gtagaagtga tttgtacaac
    21301 agatgatccc gcagacgact tgcactatca tcagttgctt gctcatgaag aaccagattt
    21361 gaaggttcta ccttcattcc gtccagataa agcactcaat attgaaaaag aaggatttaa
    21421 tgcatgggtc acactactgg aagaagcagc tgaccaaacg atcaccacct atcaagaact
    21481 gatagaagtc ttagggcagc ggatagaata cttccatcaa catggatgtc gtgtttccga
    21541 ccacgcttta gacatcttac gctataaaga agcggatgaa acagtattag aagagatttt
    21601 tcaaaaagca cgagctaaca aactattatc ttcagatgaa atcgatgctt atcgtacaga
    21661 aacactgata catttgaccc acttctatca ttctcataat tggacgatgc agctgcacat
    21721 ccatgcttat cgtaactgta atacacaaat gttggaaaaa ttagggcccg ataccggcta
    21781 tgatggaatg aatgatcttt ctttgactat tcctctgcaa aaattattga accgtgcaga
    21841 agaaacggat cagttgccaa agacgatcct ttattcattg aatccgaatg actatcctgc
    21901 gattttagct ttgatgggct gtttccaaaa agaacagaat ggaaaactgc agttaggttc
    21961 tggatggtgg tacaacgata ctcgagctgg tatgcgcgat caattgacac aatttgctga
    22021 tggtagtgct ttggggaatt ttgtcggcat gctgacagat tcaagaagtt tcctgtccta
    22081 cactagacat gaatatttcc gtcgtgttct ttgtgaattt ttaggcgaaa tggtcatgcg
    22141 aggagaagca cctgaagatg aacaactttt aggaagtcta gttcaagcga ttagttatac
    22201 aaatgctaaa aattactttg gcttttttgc ctaattgatt aatagtaaca gtaacgaatc
    22261 aaaaacagga tagtgaaata acggaatccg tttatccacg ttttagagaa gaagacggac
    22321 caaatttgct ccgtcttctt ctcttgctgt tctgcttcat tgcctataaa cctttatgaa
    22381 agaagagata acatgcctac tatccatatt gccggtgatt ctactgcggc agaaaaagat
    22441 gaaaacaagc gtcccgagac aggttgggga gaaaaaatct ctgcttattt ttcacccgag
    22501 atccagatca ataaccaagc aaagaatgga cgcagcacca aatcatttat ccaggaagga
    22561 aggttagccc gtctagcttc acagtttcag ccgaatgatt tcttatttgt acaatttgga
    22621 cataacgacc agaaaatcca agaagaaaga ggtacgcttc ccttcggtag ctatttaaaa
    22681 aatctgcaga tattttttga atgcgcaaaa aaagcgaacg tccaaatggt cttactgaca
    22741 tctgtgacgc gacgagatta cttagaaaac ggaacattga atccagatat acttggagac
    22801 tatccaaaag cgatgcgtgc atttgctgag aaacaccata ttcctctttt agatgtgttc
    22861 tcaagatcac aagaattgtt ccagaccttc tcaaaagaag agacaaaaaa attctacctg
    22921 catcttatac ctgatacttg taaaaattac ccagaaggac taaaagacaa tacccacttt
    22981 agtccagaag gcgctgataa agttgcccga ataattgttg aattgataaa agaatcccgt
    23041 ctgcctcttg ctaattattt acaaaaagga gtttgagatg tttccattaa atttaggcat
    23101 ccgtgcccac gatttagatg ttcgaaatcg tgaagaaatc gttcaaaaag tatcacagct
    23161 tcacttgtcc catatccaat ttgcaccaca aaaagcattt cctgaacaac ttaaattaac
    23221 tgacatgaca cttgggagtg ctgcctattt tggtgaatac ttccaaaaaa acaatatcga
    23281 actttccatt ttaggatgct atatcaacct atccagcaaa aatcaagaag ttcgtgcaca
    23341 agcattggca actttcaaac agcaaattgc tctgtgctct gcctatcaag ccaagatgat
    23401 tgctacggaa accggtagtg tctctgtcgg ttacacaaaa gaaaatttta ctgaagaagc
    23461 ttatcagatc gccagaaatt ctatcattga attagtcgct catgcagaaa attttggcgt
    23521 aactgttgct gttgaagcag ggatcaatca tccaatctat aactatcagt tagctaaacg
    23581 tttaatcgat gaagtagctt caccaaattt aaaaatcatt ttagattgtg cgaatttgat
    23641 gcataaagaa aatcatgctg atcaggaaaa agtgatccga ggtgccttgg acaatttaca
    23701 tggttatatt acagcgcttc atctcaaaga ctatatcatg gaaaatggaa agatccgcat
    23761 ggtacctgtt ggcaagggct gtttagattt ccgcccggta ctgcattata tcaaatatga
    23821 gcgtcctcta atgtacgcca ctcttgaagc tactcctgag atttatgttc ctgaagcaat
    23881 agaacacttg cagtttattt accaagattg ttaaggagaa gattatggag gaacgaatac
    23941 agaaaagtta tcaaaaatat catgcaaatg cttttcagac attgctgcat ctttatcagg
    24001 gaaattattg gcgttttgcc atgtcaggac tcttttttat tatcaaacat cttccatctt
    24061 ggttaatgcc gatcgctatt gccaatgtaa tcaatgcagc ttcatcgaaa gattcttctc
    24121 agttgcaggc catttatttc aacgcttttt tgatggcttg tctgattggt caaaatatat
    24181 tgacgaatta ttttcatgta aaataccata gcacttcgat ccgtcaagta gaagctggac
    24241 taagagcgac ccttatcaag aaaatccaag aactatctat tccttatcaa aaagaattgc
    24301 aatctggacg aatccagtca aagatcatcc gtgatgtaga agctatccaa acgttgtcct
    24361 ctcaagtatt tgtcagcgga ttaaatatcg ctgtcaattt attcgtagct ttaggtatca
    24421 cgctttataa aagtcctgtt gttttcatgt ttttcttatt gactgttcct ctcgccgcat
    24481 tcgttactct ctttttccgt aaaaggatca gacagtccaa ctcagacttc aggcaagaaa
    24541 tggaagagac ttctgcaaaa gtgatcgaag tgatcgaatt agcaccgatt gctcgagctc
    24601 atgggttaga aaaacacgaa acaactcgct taaataagca ttttactcaa atctatgaaa
    24661 aaggattcca cttagatatc ctgcaatctg ttttcggatc agtcagctgg gctagttttc
    24721 agatatttca gctgatttgt ttgattttca ctgggatatt agctttacaa gggcgcatcc
    24781 aacccggcga tgtcgtgatg tatcagacgt atttcacgac gatcgtcaat tccgtttctg
    24841 gaatgatcac gctgattcct acgatttcaa aaggcatgga atctatcact tctattggcg
    24901 aactgttgat tgctggcgat gtagaaactt atcacggtaa gaaaaaaatc caacatttgg
    24961 atggaaattt tcgttttgag gatgtttctt ttctttatcc ggactcactc aaaccgatta
    25021 tcgatcattt ttctctagag gtgaaagctg gagaaacaat tgcttttgtt gggccatctg
    25081 gctctggaaa atctacactc cttaacttat tgattggatt tatccagccg acttcaggaa
    25141 aaatttttct agatgatcag cctttggatg aactggatat gcgaagtgtg cgaaattatt
    25201 tagccgttgt tcctcaaacc actttgttgt tctccgcttc gatcaaagag aacatcactt
    25261 atggcttgaa aaacgtatca aaagaacgtt tagatgaagt catcgaagct gcgcaacttt
    25321 cttcactgat tgaccagctt cctgatggtc tggatacctt agtaggcgaa catggcaaca
    25381 agctgtctgg tggccaaaag caacgtatct ccattgcacg agcgttgata cgtcagccaa
    25441 aaatcatttt gctagatgaa gcaacctcag ctttagataa ccagtcagag aaaaaaatcc
    25501 agcaagccct gaattacctg acagaaacac ccactacttt tattgtggct catcggcttt
    25561 caacgatcaa agaggcagat aaaatcgttg tgatcgatga aggaaaaatc gttgaaatag
    25621 gtacttacga aaaactgtta gctcaaaaag gctatttcta ccgcttgaaa aatgaatgac
    25681 aaaaaatagg agctatgaca agacttttgt cgtagctcct atttctgaat aaacggtgtt
    25741 ctcctgccaa ctcctcggtc acaagtcaca atagtcatca aacatgagaa gctgtttttt
    25801 gaaattgttc cttgtaactc agagttaaac ggtaggctca cgacctcttg gtaaatggtg
    25861 ttcaagagac agcctatcgg caataagaag caattttgtc atcgctcaag gccgaacgtc
    25921 tctttcacac cctctgttgt ttcataaaat agttttattc gctgtaaagg tcgcgaactg
    25981 gtgtcatgat gatacggttc aagtcattga tgattgtact gaagccttgc tctttttgca
    26041 tcaagtcatt gattacttct tctgattgga ttttcacagc catttcttgt gctttcattg
    26101 catcttcgtc gctgaactct tgtccactca tttgtttttc ttgtaattcg atttgcatcg
    26161 cttggaagtc tttgaacagt ttgtgtgctt cttcgtttgc ttgtactttc tcgtacgctt
    26221 ctttcaacgc tttgaattct ggtaattcac ggatctcacg ttcgatttga ttcgcgctat
    26281 cgtagatgtt tgacatgtta tgccctcttt tcttttagta gttactctct attgtaacat
    26341 ggggttattc gttttcaaag gggctactct ccttgatatt tccgccagag gtctttagcc
    26401 gcttcaccaa ttccctgaac gccttctttc accatatcgc cgaagttttt cagcccttct
    26461 tttgcttgtt caccatattg attcaagtta tcttgccaat ttccagactc tccattagaa
    26521 tttcctgtgt ttccaacttg atctgcagct accacttgtc ctcctgtggc ataggcatct
    26581 gctacactaa atcctgtttt tgaagtatac ggaaggatgc cttctgccgc tgatttgaag
    26641 actttcccga caacatttcc acttgtccct tccaaatagt gcagcttgct ggattcttcg
    26701 taacccatcc atgtcgaaat cactacgtct ggcgtgtaac cgatcatcca ttggtcattt
    26761 gcttttgtgg catcgaaatt agtttctgtg gttcctgttt tccctgctat cgtataacct
    26821 gcaggattcg cagcagccgc tgttccattt gaaaatgtac caagaagcat gctggtcatt
    26881 tcatcggctg tttcctttga tatgacctgt tttggttttt tgtttgtgtt atcgacgatc
    26941 accgctccag tcgaatcaac gattttcgtg ataagatgcg gcgtatacat ctgaccatca
    27001 ttggcaaatg cactgtaagc acccgccatg atcaatggag atactccttt ttccagtcca
    27061 ccaagggcta agccataata cttatcagat tttgctaaag gcaggccgaa ttcttctgct
    27121 ttatcatatc ctttttgtaa accgatctca tgcaaaagcc atacagtagg caaattcaaa
    27181 ctttgtgcaa cagcttgata catcggtact tctccttggt atgtcccgtc aaaattatgc
    27241 gcatcgtaat aactttgcgg ttcgtctttc aagatactgg aaggcttgta tccagcttcc
    27301 agtgctggtg tatatacgct taatggtttg atcgttgatc ctggtgaacg acgcatctgt
    27361 gtagcaaagc tgaatcctct aaagacatga tctcctcttc gtcctactag tgcttgtaca
    27421 ccacctgtat tcggatctaa agctactgaa gcagattgga ccatggcacc atctgaagca
    27481 tttgctggga ataatgcact gttttgataa gtagcttcta attgcttttg ataatcctga
    27541 tctaaagctg tatagatttt atagccttta ttcatgacat cttcttcatc aagaccatac
    27601 tcgttaactg cttcatcgat cactgcatca aaataataag gataacgata accagaatct
    27661 gaattatcgt atgtgtcatt aagatacgcg ctgatatctg tctggctttc tgcttgttct
    27721 tgttcttttg tgatttttcc gttttcttcc attacactta gtactgtgtt tttccgatca
    27781 ttcgcatttt ttgggtgatc gatcggatta taaataccag gacctttcag cattcctgcc
    27841 aaagtagcag cctcagacag cgtaacattc gttgcatcta ctccaaaata ctttctggaa
    27901 gcgtcttgga caccccatac cccgttgcca aaataggcat tgttaagata catggtcaag
    27961 atttcttctt tgctgtactt cttttcgatc tcgattgcca aaaataattc cttcgctttt
    28021 cgatcaaacg tttggtctaa tgtcagatac gcatttttgg caagctgctg agtgatcgta
    28081 cttcctccgc cccctgatgt tccgccagac aagaccattc ggactgctgc acgggcaatc
    28141 cctttgatat caaacccgtg atgctcgtaa aaattcctat cttctgtcga gattactgca
    28201 tcttggatat agggagaaat cgcgtttaat tctacatatg ttcctttttg ggcgtacagt
    28261 gtgccagctt cttcgttttt cttatcataa atgactgtcg attggcttaa tcctgatttt
    28321 aacgtttcga catttgcttg ttttgctaaa ataaagaggt agatacttgt cactaagaca
    28381 cagatcaaac caatcagcaa aatcaatttg ttcacatgat attttttcca gatgcgcttt
    28441 cgccatcgat ggaattgtcc gagatatggt ctgatccaac gccaaaaact gatcaaagca
    28501 gcttttatct tttctaaaat gctttgaaaa tccatttgct tcttctcctg tctatttgat
    28561 tcaatcgtct tattttacca tagtgaaaga gtgttttctt cctgcttaag ttgtagttca
    28621 atatgttttt ttacgttagt gcaagtagag taacataaga atgaacagaa aaatgtaagt
    28681 tttttcttaa atatgtttta aaaatggtac aattagtctg cgattgcact gaaaggagta
    28741 cacttgtgaa aatcacgatc actttaccta tgaaccatcc tgaaacaaca attcgcgact
    28801 tgcttgaaaa ggaatggtta gttcctagaa aggttcgaca ctttttgcgt atacgtaaaa
    28861 acgtttggat caatcaacaa cctgctttgt tccatcatga agtaaagggc aatgaccaga
    28921 tcacattgtt attcgaagaa acagattata attatcaaga agttcaatta ggagatgcga
    28981 agcacatccg tgttttatat gaagatgagc atttgattat agtggataag cctgcaggaa
    29041 tgaagactca tccaaatgaa ccgacagaaa acagtacctt actcaatcat ttagctgctt
    29101 atctcgacac taaacagcaa cgaccttacg ttgtccaccg cttggacaaa gaaacaagcg
    29161 gtgccgtttt atttgcaaaa aatccatttg tcttgccgat acttggaaga atgttggaac
    29221 aaaaaatgat ctatcggaga tatcaagcca tcgtatgggg agaaattgcc aaagatatga
    29281 ccatcaccaa taaaattgga cgagatcgcc atgatcggag aaaaagggtc gtagatgata
    29341 aaaatggtca gagcgccttg acacatgtca ctgtcaacaa agtactttct gaacaaacac
    29401 aagtctattg tgttttggaa accggacgta cacaccagat cagagttcat ttagctcata
    29461 agggacatcc gatcgttgga gaccctctct accaaaatag aacagctgaa cgactgatgc
    29521 tccatgcatt agaactgcac atgatccatc catttactca agaaaaaata gtcggtaaag
    29581 ctttacctgg attatgggaa tagaaataaa aaagaacaga gtcttatcag cagataatga
    29641 taagactctg ttctttttgt gtaatttgtc tgcttacttt tgatgttcgt gttctgctaa
    29701 cagcaaacta ccgataatcc cactttcatt cggcagtccc caacgaacga tatattcact
    29761 tacttgaggt gtttccatat aaccacccat caattcaaca aattgctgac ggattttttg
    29821 aaggagatga tcctgattca ttaccccacc gcctaaaata atcttttcag gtgctaacgt
    29881 caacgtatag ttgatcagtg cttgagcaat ataataagcc tgaatagccc aaacaggatg
    29941 atcttgcggc agatcttgcc ctttgatccc tgtgcgtgcc tcaatagacg gtcctgctgc
    30001 taatccttct aaacaatctt tatgataagg acaagtacct tcataatggt catctggatg
    30061 gcgtttcacc caaatatgtc ccatttcagg atgtgcgatc cctgaaaaaa tttcttcatt
    30121 gatcacgaca cctgcaccga ttcctgttcc cacggtaagg taaatacaac tattttttcc
    30181 ttgtgctgct ccttgcttca attccccata tgcagcagca ttgacatccg ttgtccaagc
    30241 agcaggaata ttgtagcgtt ccttgattgt gccaagaaaa tcgaattgtg cccacccttt
    30301 tttgggagtt gccaaaacgt atccgtaatt atcttttgtt ttgtcgatac caatcgggcc
    30361 gaatgagcca atcccgattc ccttcaattc ataacgatca aaaaattcaa atacctgatc
    30421 caatgtttct tctggtgttg tggtagggat acttaattt
//
LOCUS       DAZKNH010000026        29138 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000026 DAZKNH010000000
VERSION     DAZKNH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.026"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..867
                     /locus_tag="ACG8MG_001096"
     CDS             <1..867
                     /locus_tag="ACG8MG_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0375160.1"
                     /translation="LAIDGLAPGAYQLVETQAPTGYELDATPIEFEIERSQTAVVELT
                     KANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPG
                     VYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVL
                     QGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFK
                     IEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLV
                     FISIKFRKKRNI"
     gene            complement(1220..2068)
                     /locus_tag="ACG8MG_001097"
                     /pseudo
     CDS             complement(1220..2068)
                     /locus_tag="ACG8MG_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2221..3333
                     /locus_tag="ACG8MG_001098"
     CDS             2221..3333
                     /locus_tag="ACG8MG_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0375161.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3411..3704)
                     /locus_tag="ACG8MG_001099"
     CDS             complement(3411..3704)
                     /locus_tag="ACG8MG_001099"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFQ0375162.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3872..4186
                     /locus_tag="ACG8MG_001100"
     CDS             3872..4186
                     /locus_tag="ACG8MG_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFQ0375163.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4207..6186
                     /locus_tag="ACG8MG_001101"
     CDS             4207..6186
                     /locus_tag="ACG8MG_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0375164.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6199..6525
                     /locus_tag="ACG8MG_001102"
     CDS             6199..6525
                     /locus_tag="ACG8MG_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFQ0375165.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6541..7059
                     /locus_tag="ACG8MG_001103"
     CDS             6541..7059
                     /locus_tag="ACG8MG_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375166.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7059..8411
                     /gene="celB"
                     /locus_tag="ACG8MG_001104"
     CDS             7059..8411
                     /gene="celB"
                     /locus_tag="ACG8MG_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0375167.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8495..10000)
                     /locus_tag="ACG8MG_001105"
     CDS             complement(8495..10000)
                     /locus_tag="ACG8MG_001105"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFQ0375168.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10092..10682)
                     /locus_tag="ACG8MG_001106"
     CDS             complement(10092..10682)
                     /locus_tag="ACG8MG_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0375169.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10861..12507
                     /locus_tag="ACG8MG_001107"
     CDS             10861..12507
                     /locus_tag="ACG8MG_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0375170.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12556..13275
                     /locus_tag="ACG8MG_001108"
     CDS             12556..13275
                     /locus_tag="ACG8MG_001108"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0375171.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13754..14701
                     /locus_tag="ACG8MG_001109"
     CDS             13754..14701
                     /locus_tag="ACG8MG_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0375172.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14713..15633
                     /locus_tag="ACG8MG_001110"
     CDS             14713..15633
                     /locus_tag="ACG8MG_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0375173.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15674..17131
                     /locus_tag="ACG8MG_001111"
     CDS             15674..17131
                     /locus_tag="ACG8MG_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0375174.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17476..18090
                     /locus_tag="ACG8MG_001112"
     CDS             17476..18090
                     /locus_tag="ACG8MG_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0375175.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18083..19807
                     /locus_tag="ACG8MG_001113"
     CDS             18083..19807
                     /locus_tag="ACG8MG_001113"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0375176.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19804..21255
                     /locus_tag="ACG8MG_001114"
     CDS             19804..21255
                     /locus_tag="ACG8MG_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0375177.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21728..23881)
                     /locus_tag="ACG8MG_001115"
     CDS             complement(21728..23881)
                     /locus_tag="ACG8MG_001115"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFQ0375178.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23883..24929)
                     /locus_tag="ACG8MG_001116"
     CDS             complement(23883..24929)
                     /locus_tag="ACG8MG_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0375179.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25079..26368
                     /locus_tag="ACG8MG_001117"
     CDS             25079..26368
                     /locus_tag="ACG8MG_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFQ0375180.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26381..29083
                     /locus_tag="ACG8MG_001118"
     CDS             26381..29083
                     /locus_tag="ACG8MG_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0375181.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
BASE COUNT         9472 a         5040 c         5913 g         8713 t
ORIGIN      
        1 ctggcaattg acggtttagc accaggagca tatcagctgg tagaaactca agcgccaacg
       61 gggtatgaat tagatgcgac accgatcgag tttgagatcg aaagaagtca gacagcggtg
      121 gtagaattga cgaaagcaaa ccgacttaca ccaggcggtg tcgtgttgac aaagatcgat
      181 gaccaaagcg gggaagtcct gcaaggagct gtttttgagt tacaagatgc caatggaacc
      241 gttctgcagt caggtctaac cacaggagca gatggaaaac tggcgattga cggcttagca
      301 ccaggagtgt atcagctggt agaaacccaa gcgccaacgg ggtatgaatt ggatgcgaca
      361 ccaatcgagt ttgagatcga aagaagtcag acagcggtgg tagaattgac gaaagaaaat
      421 cgacttacac caggcggtgt cgtgttgaca aagatcgatg accaaagcgg agaagtcctg
      481 caaggagctg tttttgagtt acaagatgcc aatggaaccg ttctgcagtc aggtctaacc
      541 acaggagcag atggaaaact ggcgatagat ggcttagcac caggagcata tcagctggta
      601 gaaactcaag cgccaacggg gtatgaattg gatgcgacac cagtaacttt caagatcgaa
      661 aaagaacaag aagtggcgat ctttttaaca aaagagaata gaaaagttgc tgatagctca
      721 gatataagag gaaacaatcc aacaagcagt ggaaataagc atcttccaaa aactggagaa
      781 accatcatta gccagtttat attgtcaata ttgggtgtgc tacttgtttt catcagtata
      841 aaatttagaa agaaacgaaa tatatgagct aaaataaaaa attgaataag catagttttt
      901 aaaatcaact agtacccatc aattgaatgg aaactaccga ttttagaagg ggtatacaaa
      961 caaatattag tatcggagta gagaaggttg aacccaacca gtaaataagt aaagacaatt
     1021 ttgtgctgcg ttgagaagtg aaaaataaac ctttgtagta atactagtta ataaaaaata
     1081 tacaaaaaca ctgcgaaaat cattcttgag tttcgcagtg ttttttgcct aatgactcaa
     1141 gtttattggg taatactttg attttctttc tacaaataat cagcgatcag ttgtaatttt
     1201 ttctctagtt ttaatggctc tttgtcaata aggagaaaaa caagtcgggt cttttgtctc
     1261 ggaaagcttc aaccaactcg tgaataaagg ttacgcctcc ttgaaaggcg tggaaaaacc
     1321 aagtaatcga tcaatcacca atttcgcatt tggtagaaca tgtttggtga gctgaaagta
     1381 ggtggcgttc atgtctgtaa ctaagaattc tatttcttta ggattagtgc agctaaggaa
     1441 atagtttgtt aatcgaggaa ttttttgtgt aggtaaattt tctcgattaa tttttctgtc
     1501 tcgccatccg cggaaataaa actcagttta tcctcaacgg aaacgtgaga acgaaattca
     1561 caaccctcaa cactcaagac agaattcttt tggaagactt tggtaaatag cttctcaact
     1621 ctttcaatgt gcgaataacg gttgtcaaag atacctaaca acttttcgaa ataaaaaata
     1681 acgacacttt ttcagtcagt aaagaagtga ttttatgtct gacatgattt gaaatcgagt
     1741 gccttggacg gataaaataa ctttgagcgt tccaatggct tcgacagttt ttacaggtat
     1801 agcgctgctt ttttaggcgt ataaccattg gcatatgatt atattattca aagtggacaa
     1861 tggtttcctt cttcttgttt ttcaccacca ttaatttcct atttccatct accacagcag
     1921 aaccacaatt tctacagaca catggagcag ggaagagaac tgcagcaacg atcaatgttt
     1981 ttttcttctg taaggtttcg taagagacct ctgtaatcat caaatttttg tctattgatc
     2041 tcagaatttt tttgatagaa ccattcataa agcatatcgt tctctcagtt gtttattttg
     2101 tggtgattta atcatacaag aaaacgatat gtttttttat aaaaacatga ataggaaaaa
     2161 tagttgacaa gaaaatgaca acgccatatg atgttccata gtatttacaa cctgtcatag
     2221 atgactgtaa aaaataaaag taaaaagaaa ggacgacaac aggtggattt ttatcagtct
     2281 ctacaactag atccgtttat tttaaaacaa aaaatacgag aagcagcttc taaaaaagaa
     2341 aagtgcatgt atatttgttc attgttttta cgatcattat tcatcgtatt gtttgccatt
     2401 tgttttatca ttataattac aacattattt gaatcgaagc ataaaccata cgctgtggtt
     2461 ttattctgta tgttgatgag tattcgtttt gttgactttg gttataagat ttcccattcg
     2521 atcataggtt tagcaatcgt catgttctca ctattagtag caccgtatgt ccagctgatc
     2581 aaatggtcag ttatgggcat gttgattcat tttattttac taagcagcat attaatggca
     2641 accgctagtg atcccaaaat ggggaatgct tctttgtacg gcttttcata tctctttatt
     2701 gtttattcgt tacctaaaga ttcgttgaac aagaatttct ttacacaaac aagtagctta
     2761 ttatttttgt ttttttgttg gttttcagtt ctcctttaca gaaaacatcg agaaaagaac
     2821 aaagacaaaa gcttgtttaa agagattttt ttgaaaggca tgtattcgca ggaaaaaatt
     2881 tggatgctca tctatgcatt tggcattagt cttttgatcg ttgcaggaga gtatgtccct
     2941 tttcagcgat tgatgtgggc agggtttgca ttttcttcta ttgtatctag ctatggattg
     3001 atgtcgattg gtttcaaaga acgagcagta gaccgaatca ttggctcatt gattggatgt
     3061 gttttattta ttggtatcag ccaatttatt ccttttaact gggtcggtat attaggtggt
     3121 ctagctttag gtgtttgttc tacctataga tataaaacag tttttaattc ttttggtgct
     3181 ttggcaatca ctgccagttt attcggggtt ccaggagcag taacgatccg catttttgag
     3241 aatatccttg gggtatgttt aggaatcatg tatattgggg tgactgaaat acttatccga
     3301 aaaataagag aaaaacacgg tttgaatcat tagatgagat cgaataaaaa ataaggaaaa
     3361 agtgaagata cacatggatg ataaattgct atcattccat gtgtatcttt ttacggtaaa
     3421 tcagtgaaac gttcgacttt catttttaat ttatactttt tacggagttc gtttatttga
     3481 gccatcattt cggatttgtg atgcttttcg attgcttctt catctttcca acggtcgatc
     3541 agtaaaagag attctggatc ttctagtgga aagtaatagt catatttctc gtttccttct
     3601 tctgcacgaa tcgctttgac aatgtttctt tcgaccattt cttctgcaaa ttttctggct
     3661 tgtccatttg tacttgtata caaaatattc actgtgatac tcactaaatc ccctcctgaa
     3721 aaaattctat cactagtata tcacggtgcc agaaattatt attcaagata tgatacacat
     3781 tttttccgca atgctacgga aatatatatt ttgaatagaa aacgcttaca agatatgctt
     3841 ggtttataaa aataaaagga gtgattgatt tatgaaaaaa gcattgatta tttgtgcggc
     3901 aggaatgtca tcctcgatga tggcctcaaa gacaacggat ttctttaagc aaaaaagaga
     3961 agaggtactc gtagatgcag tttcagctac agaaggcgat aatatgatta agaatagtga
     4021 ttttgattta tttctgatca gcccgcaaac gacgatgttt ttggataaat tcaaaaaatt
     4081 aggagaatct gttggaaagc ctgttgttag tatccctttc caagcgtatg tcccaatccc
     4141 tacaggcatc cagaagttag cagaggtgat cgaagagaac atatgatgga ataggagtgg
     4201 atgataatga aacagaagga aaaattgctg ttgagatatt tgattgatca caagaaggag
     4261 tatgtcacca gtcaacgttt agcttctgaa ttgttattat ctgatcggac aatccgtaat
     4321 tacttgcagc ggatcaaaga gttagtagaa aaaaacggag gtaagatcat tgctaaacca
     4381 ggttatggct accaactgca tattttacaa agactgacat ttgacttgtt tttgagtcgg
     4441 caagaaatag tacctggata tgctagagaa ccgcaaggat tttatgagtc cgaagatcga
     4501 caaaagtata tattgaacca attgcttttg gaagatgcca ttctttttat ggatgatttg
     4561 gcagaagagc tttatatcag tcgttccagt ttgacaaagg acatgcaaga aatcaaggag
     4621 cgattgattc catattcact aaaaatcgtt tctaagcatg gtcaagggac atggatcgaa
     4681 ggggaagaaa gaaatcgacg gcattttatc atggatactt tttttggaaa ccattatgga
     4741 aattcattga aagaatattt agggcaaagc cgttttttcc cagacatcag ttttgaagag
     4801 ctagtcatta tcattctcga tgaaacaaga gaagctaagt taaaagtttc ggattttatc
     4861 atacagaatc tgactttaca cttggctttg gggatcaaac ggctccgaga agggtttgaa
     4921 atcaaggaat taggcatcga acaagaagtt tctaagcgag tagagtatca agtggcgcag
     4981 cggatcgttc aacgtattga ggtaatcgca ggtgtatcgt ttcccgaaga agaaattgct
     5041 tatttgacat tacatctcat ggctaagtcg aatcacaaag aaaatcaaaa tgatcaggaa
     5101 ctatcaaagg aattagcgaa agttctgaaa agattgtctc aggaatttca tttttcatta
     5161 gtagaggatc tccagctttt caatggacta ctcgatcatt tgtcaccgat gatgattcga
     5221 ttgagccgag gtattcgcat tgagaaccca ttgactgagg agataaagaa agaaaatccc
     5281 aaagtctttg acgcagtaaa aaggcatttc agcaacatgc cggctttgaa aaactatacc
     5341 atcaatgaag atgaatgggc ctatttggct ttgcatctca tggcggcatt ggaaaaagaa
     5401 agagctgcgc ataaactaca tgcattgatt atctgtgcaa cgggttacgg cagtgcccaa
     5461 ctattaaaga atcgcgtagt cagtgaattc ggtaaaaata ttacagtggt aagtgtaaaa
     5521 ggctattatg agatcaatga aacaacttta aaaggaatcg acctgattat ttcttctatt
     5581 gatctttcga caatgttttt caaaattcca gttttacatg tcagtatttt tttgaatgaa
     5641 gatgatgttc gtaaaatacg aaaggtagtt ggagaatcta taccctatca ttcctcagat
     5701 agaccgatac cagcattttc tctacagcaa aaaaagcaga tttacaccga acagttgtct
     5761 gaaaaatttt tcaagactta tacgtacgaa ccgaaaaagg aagaaattct tcatgatttg
     5821 ctgcaaaagc tgagcataaa tgaagaagaa ggatatataa gagaaatgcg aaagcagatc
     5881 cagcaacgtg aaaatatggg acaaatcata ttcagtgata cagtggcagt cccacatccc
     5941 gctgttccag ttggaatcga aacaaaggta gccgttgcat tgattccttc tgggatgcag
     6001 tgggatcaat atcaagaaat tcattttgtt tttttagttt cgccttctta tgtcgaaaat
     6061 gaaggaatta cggtcgtgac taaagcaatc gttaggttag ttgaccagct aaaagtacag
     6121 caggaaatct tagctgaacc tatttttgag aatttcagca gtaaatttat caaactgatc
     6181 gaataaggag gaaaacagat ggctgaaaaa ataaacagtg aaacactaca agtgacttct
     6241 tttgatatca ttttccatag cggaaatgca cggacactga tacatgaagc atttcgctcg
     6301 atgcgtaaag agatgtttga agaagtagaa gagaagttgc gacaagcaaa tgaagagatt
     6361 cttcatgcgc atcagtcaca aactcaactt ctgaaagaat atgccagcgg gacaaaaata
     6421 gaaatggaaa ttatcatggt ccatgcacaa gatcatctga tgacgacgat gacattattg
     6481 gaagttgcac aggaaatgaa ttatttgtat caaaaaaata aatagaaagg aaggatcatt
     6541 gtgcaggata cagtaaaaag gaaaatcgaa ttagaaaaaa agcaacttag tttgaaaaac
     6601 atgtatttca atcgttatct aatcgtacgt tacctgactg cctttttctt tttcatgaac
     6661 atgcaatgga tgattctgct tctatcagct aaaagtttag gaagtagttt accgatgatt
     6721 ctgattttag ctattttgcc tgcagtagga gaacaagtaa agctttatcg aaaacaccaa
     6781 acgaacgttc catggacaaa gtggtatttt ttgttccaga gtgtctttaa tttattagtg
     6841 attccagtgt tgtttacctc aggatttaca ttcttgtatc cttttatggc aaataacaat
     6901 ggaggacagt tatttgtttt cattcttgtt gtaagcggta tttttgccag tgcatttata
     6961 cagtatcgtt taaagaaaat ttcactcaac caagaccaac agtatatccg tattaaacaa
     7021 tatgaaaaag cgttatattt aggaaaggag aataactaat gtttgatttt ttgcagaaat
     7081 acctgatggg acctatgggg aaaattgcgc aacttaaaat cgttcgggct gtcatggcag
     7141 cggggatggc ttctattcct ttcacgattg taggctcgat gtttttggtt ttaaatattt
     7201 tgccattacc gttcccattt ttagaaggat tttttaatgc aacttttttc aaagttagtg
     7261 acttgtacat gatcgtcaat acaatgacga tggggatttt atctatttac tttgccattg
     7321 tgtttgctta tgaattaacg tcaattgaac gagatgaaca aaaattaaat gttaatccgc
     7381 tcacgggggc attactatct gtttttgcat tctttatgtg tatccctgaa ttgatcatga
     7441 ctgatggtaa aatcagcttg attgcttcta tcaatgagac agaaactgta gtaaatggta
     7501 ttaggatggg ctcttttgtc gaacgtttag gaacttcagg aatctttaca gctattatta
     7561 tgtcgattat tgcggtcgaa ttgtattgta tgtgtgtcaa gagaaattgg gtcataaaaa
     7621 tgccagatgt tgttccgcca ggagtttctc gttctttcac tgctctgatc ccaacatttg
     7681 tcattgcgtt tgtggtgatg atcatcaatg gattattagt tgcactagga acggatatct
     7741 tcaaaatgat tgctatccca tttgggtttg ttacgcagtt gacgaataca tggcttggtg
     7801 tcatggtgat ttatttcttg atccatgcat tgtggatcgt agggatccat ggagcgaata
     7861 tcatcacttc tttcttgacc ccgatcgtat tagctaatat ggcagctaac gcacaaggcg
     7921 caaattatcc gttggcagga gaattcaaca attcttatgt aaccgttggt ggatcaggtg
     7981 cgactttagg attgatcatc tttattgcat tcctcgcgaa atctgatcag ctgaaagtgt
     8041 taggaaaagc ctcacttgta ccaggattat tcaatatcaa tgaaccaatc attttcggga
     8101 tgccaatcgt ctacaatccc tatttagcgg ttccgttttt cttagctccg atggcttctg
     8161 ctacactcgc ttattttgcc attcggctgg aaatcgtcaa accgatgctt gcacagatgc
     8221 cgtggccttc accagtcggg atcggagcat ttgttggtag tggtggtgat tggaaagctg
     8281 ctgtgttagc agtcatttgt gcaatcatcg cattcattat ctggttccca tttatcaaat
     8341 tctatgataa taaattatta aatgaagaaa gagaaaaggc agcagaatta gctagtgaaa
     8401 agttaaaatg atttctaaga aaaaaggaac tgtgacaaaa aatcacagtc cctttttttc
     8461 ttagaaatgg gaaaccacta gtatataaat accattattc ttcaatcaac cgcttcacga
     8521 ttgcataatc cccttcataa ggttcatcta caccattgat tttttgatag ggatccacgc
     8581 cttttcccgc aataacgact gaatcttgcg gagcgctttc tgctaaagcg cgtcgaatcg
     8641 cttcaggacg atccatttca taatgaacga tcaaatctgg attagtaatt gcttcattga
     8701 tttcttccgc gatttttttc ggatcttcaa atgctgggtc atctgccgtc aaaaaggcga
     8761 tgtcagctgt ttctgatagc acttttccaa aatcatgtcg acgggagatt gctttgtttc
     8821 ctgggctgcc taatacaaca attacacggc catctggatg ttcatttttg gcaaatttca
     8881 gcaatgtttt caagcttaga taattatgtg catagtccac atagacatgc gctccattgg
     8941 attggatcaa ttgatccatt cggcctggta cacgagcttc ttttaatccg tcttgtgcat
     9001 cttgactgga ggcacctgtt agtgctgcgg caatgatcgc actggctgca ttttcttggt
     9061 tgaatcctcc agccagcaaa atatcataag cagtatcgca gatagctaag cgatcttcat
     9121 gagaagataa agtaaaaccg tgagtacctt tatctgaacg gatcacttga taattgcttt
     9181 cttccgaacg accataagtg atcgttggaa tctggaatag atcggctgtt tccctaagta
     9241 aatcaaaata gtcactttca tgatttaaaa caactgtttt tgaattcaag atcaattgtc
     9301 gcttgcaata gaaatagtca tcaaatgtcg gatgttcgat cggactgata tggtcaggcg
     9361 agatattcaa gaagattccg acatctaaag tcaagccgta tacgcgttgt gttttgtaag
     9421 cttgtgaaga aacttccatc actagatgag tcataccatt ttcaactgct tctgacatca
     9481 tacgatataa atctaatgac tcaggcgttg tcaaatggga tttgaaatat gttttgccat
     9541 ctaacgttgt attcatagta gaaagcaatg ctgttttttt atttgttgtg tgatccaaga
     9601 ttgcttttgt aaagtacgca gcagttgttt taccttttgt tccagtgaat ccgatcactt
     9661 tcaatttatt ttgcggatag tcgtaaaaag ccatagcaag aagcgccatt gctttacgga
     9721 tatccgttac gatgattcct actgctgaat tttcgtatgg ttgttctgat acataatatt
     9781 gaataccctg ttcgattgct gaatccaaat attcttcttt gaaattcatt cctttacaga
     9841 aaaacaaagt agaagcatca gcttttcttg aatcataact taactgaaga aattcagctt
     9901 tttctggtac atcaagatgc cagcctcttg ttgaaacaaa ttcttttaat agattttctt
     9961 ttaatagtaa atgacggatg tcttctaaag agaagctcat tgattgatcc ctcgctttat
    10021 tcaatttctt ggtttattat agcacactct ttttaatata tcgtttgttt ccgctatgac
    10081 aaaatccagc tttattcaaa ttttcttgac acaggggaca agcggatgag tattttcata
    10141 aacaatgaac tgactgtaat agataggaga acgaatagtg tctccaacaa tccaaaaaca
    10201 taataattca aaaagacgat aagactattg gtaagtaaaa gtccttgcgg cattggaagc
    10261 cgaaaaaatt tgtggaaaat aatcggcggt atcgttatac cgccatttga ggccccgact
    10321 gtgtacaatg tgtaaacccc aagactgaac aaaagacttc ctgaaatcag tgaaaagaaa
    10381 tgtgaggaga tcatttccac ttttggaaca atctctaaaa caacaggaaa aagcagactt
    10441 cccgctacag tcttaaaaaa tactgatttt cctaatagga cagcggctag aagcagcatc
    10501 ataagattga tgctccaaac agtaactgag cgttggatag aaaaggccgt ttcaactaaa
    10561 tgtccgatgg cgcccactcc ggcggaagct atatcgtgag ggagcaagaa ccaattcaat
    10621 ccaactgtga taaagaaaag acctaaaaat ataacgggta gtttttttat ttcttgtgtc
    10681 attgatatct cctataaatg ttagccataa aaggctgtat atttctttac tttaccaaaa
    10741 aaacgaaacc gaattcaaaa aaacaattgt tcttataaaa gttatataaa atcgattgcc
    10801 agtttctgtt ttaccaatta aaatgatagt aatgttttgt agaaagttgg aatctgaccg
    10861 atggataatc aaagaaaacc agcattagat caattaacac cgcaagaaaa aatggcacga
    10921 ggctctgctt ggatgaccgc cagtaatatc atttcaagat tgctaggcgc tgtctatatc
    10981 attccctggt atgcttggat gggcgaaaat gctaaggctg ccaatggatt attcaatatg
    11041 ggatacaata tatatgcatt gtttctactg atctctacag caggaatacc tgctgcgatt
    11101 gcaaaacaaa ctgcacgcta taattcattg aatgaatatg gcacttcacg acgattattt
    11161 atccgtgcat tacagatgat gggcgggtta ggcctgttgt ttgctttgtt catgtacatc
    11221 gcctctcctt ggctagctca tgcttctggt ggcggagaag aactgattcc aacgatgcgt
    11281 tctttgagta tcgctgtatt ggtttttcct tgtatgagtg tgatacgagg gtatttgcaa
    11341 ggcaatcaag aaatgatgcc ttatgcgctg tcgcagattg tagaacaggt ggcacgtatt
    11401 ttttatatgc tattggctac attcatcatc atgaaagtca tggatggtga ctatgtgacg
    11461 gcggtcacgc aatcaacttt cgcggcattt atcggtatgc tagcaagtat tcttgtcctt
    11521 ttgtattttt tgaagaaaca tcaagcgtat acatcggcgt ttattcacta cagcgaaaac
    11581 aaaataaata ttgcaacgaa ggaattgttg ttagataccg ttaaagaagc gattccgttt
    11641 attattgtgg gttcaggggt aacgatcttc aaattagtcg atcagttcac ttttatgaag
    11701 atcatgtctg catccaccga gtattccaat gcacagttac tggatctttt ttcgattttt
    11761 agtgccaatc cagataagtt gacgatggta gttattgcat tggcaacatc gatcgcgtct
    11821 actgggttac cgttgattac agaagctgtt acgttgaaag atcgagtagg tctggcaaaa
    11881 ttgacaagcg gaaatttaca gttgttttca ttttttatgt ttccggcgac atttggtgtt
    11941 atgttacttg cctatccgct aaatacatta ttttatacac cggattctct aggaagtaat
    12001 gtactgatcc aagcaagttt tgtaggactc ttcctaggac tatatatgct agtttccaac
    12061 atgctgcaag gaatgtttga aaacaaggcg gcaattaatt atttggttat tgggtttcta
    12121 gtgaaactcc ttcttcaata cccagccatt cgtttatttg aagtatacgg ccctttattg
    12181 gcgacaatga tcggatttgc tgtgtcttgt accttgatct taaagagaat ccatgaagta
    12241 gcccgcttta aaccaagttt tgtctggcgc aggacattgc tgatctttat tcttaccttg
    12301 attatgcttt tggcttcgtg gatcacaaag atgatttttg gtgctttttt aaacgaagat
    12361 agcaaattcc agtctctatt gttgattctt gtagtagcag gtataggggc tttagtgtat
    12421 ggttatttag ctttgaaaat ccgcttggca gaaagactac taggaccagg aatagcgcgt
    12481 ttacgacata aattaaaaat aaaataagtg aatcagaaga gctagattga tgctcttttg
    12541 aaatggggga aacgcatgcg tttagataag tttttagcag atatgggact tggcagccgc
    12601 aaagaagtca aaggattaat caaaaaagga acgatcgaag tcaatggaca agtggtgaaa
    12661 agcgataaag cgcaagtaaa tgaattcgaa gatgcggtta cttatttagg agaaatcatt
    12721 acgtatcaaa aagattttta ctacattttg cataaacctg caggagtcat ttctgctact
    12781 caggataatc atgatcaaac cgtaatcgat ttattggaag accaggatta tcgggaagat
    12841 ctttttccag tcggccgatt agataaagac acagagggcc tcttgatttt aacgaatgac
    12901 ggggtgtttg cccatcagtt actatcacct aaaaaacatg tggagaaaga atatctagct
    12961 gaggttagcg gtataatgac agaagaagat atccgtttat ttgcagaagg actgattatt
    13021 gatggcgatg agcagacatt gccggcaaaa ctccaaattg atcaagtaga tcaagagaca
    13081 gaaacttcaa ttatccgtct gattttgcat gaaggaaagt ttcaccaagt taagcgaatg
    13141 gtcaaagcag taggaaaaga agtcttatat ttaaaaagaa tccgtatggg aaaattttgg
    13201 ctgcctaaag atttgaataa agggcaatat cgacccatga ccaaagaaga gctagaacaa
    13261 gtgagagaaa aataattgaa tggatagcta aaagaataag cagagtagtt gtagcgccat
    13321 ctgaaaagca gacttgtccg tctgactttc ggatggcttt tttgtgaaat ttcggcttat
    13381 ttccgtagaa gctacttctg gaacaccgtt tgttcggaat aagggactgt gacaagactt
    13441 ttgttacagt ccccattgtt ccttgtgact cagagttaga tagtaaggac acgatctccc
    13501 taatactcag gccaaaacgc tgaattcaca acctctgata aacggtgttc aatcagctga
    13561 ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttcccg tttttttctc
    13621 taatactcag gccaaaacgc tgatttcaca acctctccta tactttgttt cattttccct
    13681 atactttgtt tatttattct cattattgaa agcgctataa ttacgttgcg aatgaatcga
    13741 aaggggtttt tagatgaaaa aacgaaaggg gttcaatcat ttttttatga atctatggcg
    13801 ttataaagca cttgtcctga tggctgtacc gggtatgctt tggatgattt ttttctttta
    13861 catacctgta atggcgaatg tcgtagcatt caaaaatttt catatttcag cagatgggtt
    13921 cttggctagc ttgagggaaa gtccttgggt gggttgggaa aatttcaaat ttttattttc
    13981 ttcggatcaa gcatttctca tcactaaaaa tacaatttta tataatgtag catttatcct
    14041 tttgaatctg ctgatctcag tcgtatttgc aatcattatg agcgagttga gaaataaacg
    14101 actcgttaaa gtttatcaaa cgatgtcctt actaccttat tttctatctt gggtcattat
    14161 cagttacttt gtttacgctt ttttaagtcc tgacaaagga atattcaatc agtggatcac
    14221 tggacatgga ggagaagctg tcaattggta taatgaaccg aaatattggc cgtttatctt
    14281 ggtgttcatt ggtacatgga aagggattgg ttataacagc attatttact ttgcatctgt
    14341 tatgggaatc gatccgactt attatgaagc tgctatggtt gatggagcta gcaaatggca
    14401 gcagatcaaa catgtgacga taccacaact tattccacta atgacgattt taacgatttt
    14461 agccgtagga aacattttcc gagctgattt tggcctgttt tataacattc ctaggaattc
    14521 cggtgcatta tatgatgtga ctcaagtttt ggatacgtat atctacaatg gcctgacatc
    14581 cacaggagat tttggtatga cggctgctgc aggactttac caatcagttg tcggctttgt
    14641 cttgttgatg atcacaaata cgatcgcccg acgttttgat agcgattctg cattattcta
    14701 aggaggaaaa aaatgaaaaa aaagaaagtg gagagagtcc aagtccgctc tttcaataag
    14761 acggctaatt ttttctctaa tataatgatt gcactctttg ctatttcctg tattttgcca
    14821 tttatatttg ttatcgccat ctcttttacc gatgagtcca tattgtcgac tcacgggtac
    14881 agtttttggc cagaaaagtt cagtatattt ggttatacgt acttgttcga ccagatgcag
    14941 gataaaattt tccaagcgct tttcgtcact gtacttgtta ctgttttagg tacactgatc
    15001 aatagcacgg ctacttcttt atatgcttat gcgatttcac ggtcaaactt tccattccgt
    15061 cgttttttta ccgttttttg tttgattacg atgttgttct caccagggat ggtggccaat
    15121 tatcttgtta tgacaaatct tcttcagtta aaagatacga tttgggcatt gatcttgcca
    15181 atggctgtca gtcccttcaa tattattgtg atgcgcacat tctttaaacg ttcggtctct
    15241 gattcgatta ttgaatcggc acggatcgat ggagctagcg aactgcgaat atttgttcag
    15301 atcgttttac cgttagctgt tccaggtatt gcgacaatca gtttatttgc cgcattgggt
    15361 tactggaatg actggttcaa tgctttacta tacatccaag atgataagct agttccacta
    15421 cagtatcttt tgatgaaaat ccaaagtaat attcaatatc tgacacaaaa ttcaggagca
    15481 ggaagccaat tgacaggcgg attagcctca gttccaggag aatctgccag aatggcgatc
    15541 gtagtcatct cgacgttgcc tattgcgatc agctatccat ttttccagaa gcattttgtc
    15601 aaaggtctaa caatcggcgg agtaaaagaa taaagaaaca aaaatataaa tcataaaatt
    15661 aggaggaaga aaaatgaagt tatggaagaa actagccttt acaggtgttt cagcgttgtt
    15721 gttgggaaca ttagcagcat gcggaggttc tggttctaaa gaccaggcag aagctagcaa
    15781 ttcggacact ttgcagatgt atcaaatcgg agataaacca gataatttcg atcaattgat
    15841 ggaagtagca aacaaacgaa tcgaaaaaga gatcggtgta aaagtcaata tcaattacat
    15901 tggctggggc gattacgaaa agaaaatgaa cgtcatcatt tcttccgggg aaaattatga
    15961 tatcgccttt gcgaacaact atgtttccaa tgcccaaaaa ggagcctttg ctgatttgac
    16021 tgaattagcg cctaagtacg ctgaaaaagc ttataatgat ttagatgaag catatattaa
    16081 aggaaatctg gtcaatggaa aattatatgc tttcccagtc aatggaaatg tctttgctca
    16141 acaagtactt acttttaaca aagctttact agataaatac gatctttcaa ttgatggaat
    16201 ccaatcttac gctgatgcag aaaaagtctt gaaggaattc catgaaaaag aaccaaacac
    16261 ggcagctttt gccattggac aaggcttcaa agtccaaggg aactttgatt atcctttagg
    16321 gaatacgtta ccatttgcta ttgatctgga tggagatgat tcgaaaatca tcaaccaata
    16381 cgataatgaa acgtatatcg gactgttacg tacaatgcat agctggtaca aacaaggact
    16441 cattccaagt gacgctgcaa cgagcaataa ggattatcca ctagaaggaa atacttggct
    16501 aatgcgtgga gaaacacaag gaccgtatga ttatggcgat acaattttga caaatgcagc
    16561 aaaacaagaa cttgtctcaa aagcaattac tgttccacta aaatctacag ctcaagcaca
    16621 aatggcaaat tttgtcgtat caaatacttc aaaaaataaa gaaaaatcag tagaacttct
    16681 agggctgctt aacagtgatc cagaattatt gaatgggctt gtttggggga tcgagggaga
    16741 agcatgggaa aaagtcgatg gttcagatga taaaatcaaa ttgctagatg gttatcagcc
    16801 aaatacacat atgtctgctt ggaatacagg aaacaacaag attctatata cacaagagtc
    16861 tattacggat gacatgattg ccaaacgtga tcaatcgatt gctgatgcag aaacttcacc
    16921 gattttagga ttcagcttca acactgacag tgtcaaaaca gaattaagta atatttctaa
    16981 tgtcatgaat caatatttag atggattgaa tacagggact gttgatccag atgaaacatt
    17041 accgaaattg aaagacgctt tggacaaggc tggctatgat aaggtattga aagaaatgca
    17101 aaaacaatat gatgagttcc gacaagaata ataatgaata aagacaaagg ggttgtgaca
    17161 aaagacgtgt cacaacctct tgttccgaat aaacggtgtt caaaagccag tttctcggta
    17221 ttaagctaaa tatatgtaaa aatgtggaaa gccatattca caaaattttt cttactactc
    17281 gaaactaagc ggctatttca caaactcttg gtaaacggtg ttcaatcagc taaccctcgg
    17341 tattcgctca caaaacgaca aaatatgagg aactattttt ccgtttgttt ctctaataat
    17401 caggtcaagg tgctgattgc ccaacctttt tttgtataat gagaaagtat aaacttaatg
    17461 tttggaggag aagatatgtt agggaaggtt ttggaagagt tatttatccg gatctgggtc
    17521 attataaaac taaccttgta tttctggata tatacatttg ccggcgggat catctttgga
    17581 ttaggtgcgg catggaaaac agtcaatgaa ctattttatt tgtacggatt cgagtataaa
    17641 gaaatcacaa taaaaagagg ctggaatata tataaaagaa attttttaag aggcaatctg
    17701 ctatttagtt tatttttatc tggtactgct ttattgagtt acaatttata tttatctgtt
    17761 caaatcaaag ggctcatttt tttagctatt gattttattt tactttttgc cttgctctgc
    17821 ctatttgcta cttatcaata cagtatgatt ttagatagtg aatatacgat ctctgttcct
    17881 aatctgctaa agctaagttt tatttcggtt ttttccagct tttcgtcttt tctaaaaata
    17941 attattggta gcggtataat tttaggcgtg acttggcaat tcaaaggatt gatcctgttt
    18001 ggtgtcattg gtcttttgac ggtttggaat ggtacgatga caacacattg gcgtgaagaa
    18061 ttagataaac agttggaaag ttatgaataa gtttcggatt ctattttcaa aaggaacctt
    18121 actaaatcgt ctgttgcgga aatacgcggt tatcatgatc ggtgtaacga tgactgctac
    18181 cgttattttt agtatccata cttgggaaca aaatcaaaaa caggcagaaa acatgacaag
    18241 tgatgccgtt caaagcacaa gcagaatgct gaatgataaa acaaccttga gtaggatcat
    18301 caaaaaccag cttgtaggga attccgaaaa gatcgaaaat gtgacgacct acttaacaaa
    18361 acccatagat cagtatctga tgtatgtata cgaacagcaa aacagtaccg atgaattagt
    18421 ttctttccca aatcaaatca aagatctcta cgcaaattat gaagaactaa gtgcgatata
    18481 tatcgtgttg aatcaactac cggagtatta tgaatcttct agagaaaaca aaggtgggga
    18541 aataaaaaag gggacaccaa aattactaga cgccttttat atcaagttgc ctatcacaca
    18601 aggaggagct gagtcaggaa gtatttttat tggttttcaa aagaaagaac tagatatgat
    18661 tttagaaaat ctaacgtcat tcaatggact ctctttatac atgatttctg gaacgacaaa
    18721 tcgactatac acattccatg ataataaaat agctaaagaa acgtttgaaa gacaagaaat
    18781 gatcatcaca gagagtctga agaaaaattc acaattgccg attgacgatc tggaaaaaaa
    18841 caattttgtt caacaccaag aactgtctga tgattatcgt atattagctg tactagataa
    18901 aaaaagtgta aaaatcgaaa cgggaaaaaa tcttgccccg ctgatcattg gtatcattat
    18961 tttagacaat atactgctta tctttcttta taaaacattc aaacgatatt cacagcaagt
    19021 atctatcgtc atgcaggcca tggatcaaac agcagcaggg aatttagaga cgcggatcga
    19081 cataacaaac acggaatatg aactgaagga actttcaatc ggtatcaacg aaatgttgga
    19141 tagcatcaat cagtttgtag aagacatcta caaattagaa atcaaacagc aagacgcaca
    19201 tatgcgggcg cttcaagcac agatcaatcc acacttttta tataatacat tagaatatat
    19261 ccgaatgtat gcaatcagcg aaggaagtga agaattagca gatgttgtgt atgcgttttc
    19321 cgctttgctt cgaaacaata cgaatcaaga gaaaacgatc actttaaaag aagaactgga
    19381 cttttgcgaa aaatatgtct atttgtatca aatgcgttat cctaatcgag tagcctatca
    19441 ttttatgatc gatcctgatt tggaaaagat cgaagtacct aaatttgtga tccagccatt
    19501 agttgaaaat tattttaaac acggtattga ttttactcga ttcgataatg cgctaagtgt
    19561 caaagtcttg caggaaggca aacgggtaag gatcatcatc aaagataatg gaaaagggat
    19621 gactgaaaaa cggctaaaac agattgaaga aaaactgtct catcctaaag tggaacttca
    19681 cggttctatc ggtctgcaaa acgtgaatga acgtttacgt gccagcttcg gcagcagtta
    19741 ttatatgtcg cttgagaata atgagacagg tggactgacc gtttctatta cattcaagga
    19801 gggatgagcg tgtatcaagt gttattggta gatgatgaat acatgatttt gaatgggtta
    19861 aaaaagataa ttgattggca aagtttaggc tttcaaatag tagcaacagc agagaatgcc
    19921 aaagaaggac tagctgtact tgaacagcga cagattgatt tagtagtaac ggatgtgaca
    19981 atgccagaaa taaatggact ggaatttatt gaagcggctc aaaaagaacg tcataatttt
    20041 gaatttatga ttttatcagg ataccaagaa ttcgattatt tgaaaggagg tatgcaatta
    20101 ggcgccgtga attatttgat gaaacccgtg aataagttcg aattggttga aagcctcaag
    20161 aaaatcaaaa cacggttaga tcagcaaaat gaacaaaaaa atcaacaaga aatctatcaa
    20221 gaactgttat tcagtcaatg gttgaatgaa gaattggatg aaataagtga agaagaaata
    20281 ataggacaaa taagtgagaa gcagcatagg atagtattga ttcaactggc aagaactcat
    20341 gatactctta tcaatagatg gttgcaagaa cggcaacaaa tcttttacta tcaacgaaat
    20401 tacggagacc gtgtattgat cacgttgttg ttggaggcag atgaagttga tggcttctgt
    20461 tgttttgttc aagaaaactt tcctgatcag gaatggctga tcagtatcgg ggaagagaca
    20521 gattctttgg acaaaatccc tgaaacattc caacaagcga aagacaatct acagttgcat
    20581 caattttatg gagataaaga cgagcatgtt ttttacgcag tacaagcaga aacaaaagat
    20641 caaaccatcg atttttcaag tttcagacgc gtgctgcaaa ataaacaatt agaagtcgct
    20701 caaaaaatga tcactgattt ttttgaacag tttcagttag cagccatgat gccggaagat
    20761 atacgatatt ccagtttttt actgtttatg gagattcaac gggaattgat tgatctggaa
    20821 gatgaggaat atttacaagg aatcgaaaaa atccatcagg caaaaacggt gcaagaactt
    20881 cagcagctac ttctgtcatt catcaaaaag catcagagac agaaaaagta cagtgacaat
    20941 gtggaaaaag taatcgtaat cctgcaccag cattatcaag aaccacttac actcaaagaa
    21001 gtgtcagaaa acctgcattt aaatgtcatg tatctaggac agctgttcaa aaaagaaacg
    21061 aaaaaaagtt tttcagctta tttgaaccat ttacgtatgg aaaaagcgaa acaactgctt
    21121 ttgcacagta atcaaaacat caatgagata gcaagtgaga ttggctacaa caacaccacg
    21181 tatttctcta aattattcaa gaaaatcgtc ggcagatccc ctaaagagta ccgagaaaac
    21241 agtggagaat cataaaagaa aaagccaatg taatgaaata acttataaaa aattgtctag
    21301 aaatctaatt ttgggctgtt cttattactt gaggcaaggc agctttttca tgacctctcg
    21361 ataaacggag ttcaaaagcc agtttctcgg tattaagcca aatttatgca aaaatgtgga
    21421 aagccatttt cacaaaattt ttcttaatac tcgaaactaa acagcttttt cacgacctct
    21481 ccactaaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaatg gcaaaatatg
    21541 aggaactatt ttgccatttg tctctctaat actcaggtca gaacgctgat ttcacaacct
    21601 ctcaataaac ggtgttcaaa agcttgcatc tgcggtaata agaccaacca aacgaaaaat
    21661 atgaggatct atttttgctt ggctgttctt attacttgat gcaggacagc tttttcacaa
    21721 cctcttgtta attatagttt gataaggaaa aaggcaaatc ttttgcttga tatttcttag
    21781 ggtgagggat gatccctaat tggtaagaga tcgtgccgcc tttcagcaaa tcttgatggg
    21841 taaaatataa tttcgtatat tctttgtcca tccgtttgat tttatggata aattgttgct
    21901 gcggccgatt aggcgttgct tcaatcgtca attcctttcc atttgacaaa agcaaaacag
    21961 ccttgtcaat taatggcata ccgataacat attcaccggt tccaggcgtg acaggataaa
    22021 atcccagact attgaagata taccaagcgg ccatacttcc attgtcctca tctccagggt
    22081 aacccattgg agaatcgtcg aaggtctgag tcatcaactg tttaagcagt ggctgtgcca
    22141 tttcaggttg tcctatataa ctgaacaagt aaggatagtg aaaacttggc tggtttgaaa
    22201 tagctaattg tccaaattct atcgcggcca tctcactcat ttcgtggatc tcaaaaccat
    22261 atcctcctgg gtcaaacgtt gggggctgat tgcacagctc aatgagtttt tctttatatc
    22321 tctcactgcc accatatttt ccgatcagtc ctgaaaaatc gtgataaaca gcaaagctgc
    22381 tttgccaagc acttccttct gcataatctt gtccccaacg attagctgaa aatggttctc
    22441 tgaacttgcc atctttatct tttgaacgca taaatcctga ctcatagtca aagatatttt
    22501 gatagttcaa tgattgctgc tgatatttct tcgtcatttc ctcttcttct agtaccttgg
    22561 caacttggct aatacaaaaa tcgctataag catagtctaa tgtgtgattg actgattcgt
    22621 ggtagtgatt aggaacatag ccatatgtca agtaatcttc tgttccttgg cgtccataat
    22681 ttggatgttt gctttgaacc gtagcacctt tttccattgc cgttagaaat tcaggcatca
    22741 agtctcgtcg gatatctttt acagcagcat ctgcaatgac cgcatcgatc aatgtcccag
    22801 gcataagtcc acgttcatca ggagacagcc attttggcaa atagccactt tctctatagc
    22861 tgttcaaaaa accttccagc atttcttcgt atttttctac agcaatcaaa gaataaagag
    22921 gataaaccgt tttatacgta tcccaaaacc cattatttgt atagagaaca ccaggtttta
    22981 tagatcgact agtcgtatca tagtggattt tttgcccact aggatcaatt tcataaaatg
    23041 tttgtggaaa taaaaacacc cgatataaat tatgataaaa cgtccgcaat tgttgttgat
    23101 catggtgtgt gagctgtata cgatcaaaat aggactgcca ttgttcttga ctgtgagcaa
    23161 ggtaagtctc tttgctttgg ttttgttcta gttccaggtt ataagctgct tgttccgagc
    23221 tgataaaaga tgtcccaaat cggatagttt gtttttttgt ctgttcaaaa tacaacggga
    23281 tcgagtcgct atttttgttg gaaacagcta actcatcaat tgttaaagga tattcaaatc
    23341 tcaatataaa ataaaaagaa aaatcaggat cttctgaacc agcaaaatgt gtgatttttc
    23401 cttccactgt gtaaggatct ataacaaaga gctgatggcg tcccggaaag gataaaagta
    23461 aaccgctgtc atttttgctg taatcaatag ataagattcc gccatacatg ctaggtatca
    23521 atgtagaaca gatgccataa cgtaattgac agatcgacag atgagtcggg caaaatatac
    23581 tttcttctgg gcggtaagaa ctttgagcat ggaaaagggt gttctcctgt agttttccat
    23641 tgatcggcgt aaacagcata tgactgaaat cacccatcca aggacttggt tgatgggtaa
    23701 gacggtagcc ttgaaaaacg cggtcatcag gatgaaacca ccagctgccg ttttgatccg
    23761 tagtttgcgg tgcaaaataa ttcatcccaa aaggaacgcc agtataaggc tggcagttcc
    23821 cattagagaa atttggctga ttcgctgttg catgtctagt atctatttga ccgatattca
    23881 ttttatttct cctttgttga ttgtcttttg atgaatgaaa ctggaaattt actatcggat
    23941 ggtcgatctt ttgtctctaa ccaagtgacc aaagcatctc ccgcaagctt acctaactgt
    24001 tcaaaatctt gtgcgatggt tgtcagtggc gggtcaacaa gagctgctgc ttggatatca
    24061 tcaaatccga taattgaaaa atcttcaggg atatgataac catgctgtct caagagattc
    24121 atggtctgga tagcgacaac atcgttttca caaacaaaag cagttacttc atacttagtg
    24181 atataagaaa gcaattcatc taatgtcgcg cttttgtcat ccagtgaagt gtggaaagtc
    24241 aaaccagctt tgtccaatgc ttcaaggtaa ccaaggtaac gctgtcgaac ggattgtgga
    24301 tgagtctgcg taccaaacaa ataagcgatt tttgaatgtc cctgttcgat caaataagag
    24361 gcagcaagcg caccgccttg gaaattatcg gaaagaacgg taggaaaagg taactcatag
    24421 atttttttat ctaatatgac gacagggaat tctttcaatg aaagctcaaa aagtaagtca
    24481 agatgttgtt ctgtatcaag agcataataa atcaatccgt caaattcatt gattagctct
    24541 tttgctgttt tattttgtag aaagtcaagc gtcgtcatca tgacatcaat ctgtttatct
    24601 tgcatcacag ggttcaatcc cttgctaaaa tcaccaacag acaaatcagc gacatatgga
    24661 agaagaaaca gaatccgtcg tgtttttgta ggagaagcaa gaatctgttc cggagaagcg
    24721 gatttaacga aactgccttt cccgcgaacg cgataaataa gtcctaattg ctctaattca
    24781 gtcaatgcac gtttggaagt gattctgctt actttatagg tacgtgaaag ttctttttca
    24841 gttggtagtt ggctgtctac aggtaattca ccagttaaaa tagccgtttg caaatcatta
    24901 aatattgtct ggtacaatgg ttgattcata taaacacctc ttaatgatat atcatattca
    24961 aataatacca taatggatga aaaagtaaat aaaaagatat tttttgaaag cgtagacata
    25021 attggctgta tgaagtataa tctttaaaga tatatcaaat agaaagaagg ttttattagt
    25081 ggcttataaa gatataccaa aatcggtgca gaaatttatg gaagatatca cacaaaaatg
    25141 tggggatatc catgcggact gggcaaagaa ttttacagca gcatttgcca atactctgtt
    25201 gacaacagtc aaaaagcaaa atgacggaac caccttttta ttgacgggag acattccagc
    25261 gatgtggtta agagactcta ctgcacaagt tcgtccttat ttagtgattg caaaagaaga
    25321 tgaggaatta gctgacatga tcagcgggtt agtcaaaaag caattttttt atatcaatat
    25381 cgatccatat gcgaatgcct tcaacgaaga agctaacggt gctggccacc aaacagatca
    25441 tacaaaaatg aacgattgga tctgggaaag aaaatatgaa attgattctt tatgctaccc
    25501 aatacagttg gcctatcttc tttacaaaaa tacaggaaaa actgatcaat tcaatcaatc
    25561 ttttattgaa ggtatcaaaa aaatcttgga cgtgttcaaa acagagcaag atcatgaaca
    25621 ctctccttat acatttgtac gagacacgac aagaaaagaa gatacgctga tcaatgacgg
    25681 gcgaggtgca aaaacagctt ttacaggaat gacatggtct ggtttccgtc ctagcgatga
    25741 tacttgccgt tatggatatc tggtaccatc caatatgttt gcggtggtcg tgttaggtta
    25801 tgtggaagaa atttttcaaa cgatactcga tgataaagaa attgtacagc aaacacagaa
    25861 attgaagaaa gagatccaag aggggattca gaatcacgga cgaacggtta atcaaaaagg
    25921 gcaaacgatt tatgcatatg aagtagatgg aatgggaaac gccacagtca tggatgacag
    25981 taatgtgccc aatctgatcg ccgcacctta cttgggatat tgttctgaac aagaggaaga
    26041 ttatctgcaa acaagaagaa cattgttgag taaagaaaat ccatatttct atgaaggaaa
    26101 gtacgcaaaa ggaattggat cctcccatac gccagaaaaa tacgtttggc cgattgcatt
    26161 agctatggaa ggaatgacaa cgtcagacaa atctgaaaaa gagcggatat tgaatttact
    26221 tgtagcaact gacgcaggaa cacaccttat gcacgaagga ttcgatgtga acaatcctga
    26281 aaattatacg agagaatggt tttcttgggc aaatatgatg ttttgcgaat tagtcatgga
    26341 ttactttgat attagagtag aaaagtagga ggtatcgtaa atgaaaaaga aagtatatat
    26401 catttctcat agccactggg atcgtgaatg gtacatggct tacgaacagc atcacatgcg
    26461 actgattgaa ttgatagacg atctactaga gctatttgaa gttgatccgt ccttcaacag
    26521 ttttcatttg gatgggcaaa cgattatttt agatgactat cttcaagtaa ggccagaaaa
    26581 aagacaggca atccaacaag caatcaatga aggaaaattg agaattggac cattttatat
    26641 cttgcaagat gattttctta tcagctcgga atcgaacgtg agaaatatgc tgattggcat
    26701 ggaagaaagc cgtaaatggg gaacccctgt catgttaggc tattttcccg atacatttgg
    26761 aaatatggga cagacgcctc aattaatgaa acaagctggt atttcagcag cagcgtttgg
    26821 ccgtggtgta aaaccaattg gctttgataa tcaagttttg gaagcagaaa actattcttc
    26881 ccaatactca gagatgtggt ggaaaggacc tgatcaaaca gcaatctttg gtctgctatt
    26941 tgccaattgg tatagcaacg ggaatgagat tccagtagaa aaagaagcag ctcttgcctt
    27001 ttggaagcaa aaattagcgg atgcagaaca atatgcctct acgaaccact tattgatgat
    27061 gaatggtgtt gatcatcaac cggtacaaaa agatatatca aaagcaatcc atctcgcaaa
    27121 tgaactgttt ccagattacg agtttattca ttctaatttt actgactatc tggaagcagt
    27181 tcaaaaagat gtgccagaag acttaggctc agtagaagga gaattgacaa gtcaagaaac
    27241 ggatggctgg tatactttag ccaatacagc atctgctcgg gtttacttaa aacaatggaa
    27301 taccaaggtc cagcgacagc tagaaaatat tacagaacca cttgcgacga tggcttatga
    27361 agttagtgga aactatcccc atgatcaatt agattatgct tggaagacat taatgcaaaa
    27421 tcatcctcac gacagtattt gcggttgttc agtcgattct gtccatcggg aaatgatacc
    27481 tcgttttgaa aaagcagatg aagttggaaa gtatcttgca caagattcct tagaacagct
    27541 gaccgcagca atcgatacga ccggattccc aaaagacagc tttccatttg tcattgtaaa
    27601 tactgcagga atggataaaa ctggtgaagc agaaataacc atcgaattag aaagaaaaag
    27661 attcgctgaa ggaatacctg aacaactata tcaagagtta gaaaatttgc ctaaacgtaa
    27721 gtatcatgta gaaacaaaaa gtggggccac tataccagct attttatctg aagaaactgt
    27781 ccaatttggt tatgatttgc cgaaagaccg tttccgtgtg ccatatatgg caagaatgat
    27841 caaagtgact ttaccattag aaaacatgcc agcattttct tgggaaacat tcgcgcttgt
    27901 agaaggggaa gcgaaagcag aagaaaagga aacaatgatt catcagagtg gacgaatcat
    27961 cgaaaacggc cctctacatt tgacgattga aaaaaatggt accattacaa tggaagatcg
    28021 caaaaacaaa cgaaagttga ataatctgca tatttttgaa gatatcggcg atataggaaa
    28081 tgagtatatc tttaaacaac ctttctgtga tcagccaatc ctatccagta acaaagaaaa
    28141 ctctgaagta aaagtattag tagatacacc ggaaatagca aaaataagta ttctacagga
    28201 aatggaaatc cctgtttctg cagacgagcg tttagaaaaa gaacagcaga tggttgtgga
    28261 atttcgttat cgaaaagcag aacgatccaa agaaaaacga atccttcaaa taaaaacaat
    28321 catgacaatc agaaaagata gtaaaaaaat cgactttgaa acgactctcg acaatcagat
    28381 gaaagatcat cgcttacgtg tcttgtttcc tacaaagtta catgtggaac atcacgaagc
    28441 agatagtatt tttgaagtgg tgaagcgccc aaaccatgtg tctaaaagtt gggaaaaccc
    28501 cacgaatccg caacatcagc aagcatttgt caatattcat gatgaagagt acggtgtaac
    28561 agtcggaaac tttggtttga atgaatatga agtaacagaa gacggacaaa tcgcagtgac
    28621 gctacttcgc agtgtgggag aactaggcga ttggggatat tttccaacac cagaagcaca
    28681 gtgtctaggt gagcatcggt tcaattacag cattgaacta catggaccag aagaaaaatt
    28741 ctctacttat ttgcatgcat acgcagcaca gattcctttt tccactcaac aaatcaaaca
    28801 tcatgaagga acattgattt caaaacaaca atatttaacg ataaaaagtg aaacgtttgc
    28861 aattacagct ttgaaacgca gcaagtttag tgataaagta gtagtaagag gtttcaatat
    28921 gtctagtcat ttagaaaaat tggaaataac taaagacaat ggaaaaactg tcatattgaa
    28981 tctgcttgaa gaaccaacga agcaagcggt tgtcccaata atccaacctt atgagattcg
    29041 gacaatcgga ttcgaggagg aaaacgaatg tatggcggaa tagaagcagg cggaacaaaa
    29101 tttgtgtgtg cggtttcaga tggtggagaa attttaga
//
LOCUS       DAZKNH010000027        28637 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000027 DAZKNH010000000
VERSION     DAZKNH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.027"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACG8MG_001119"
     CDS             85..624
                     /locus_tag="ACG8MG_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375182.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="ACG8MG_001120"
     CDS             1644..2813
                     /locus_tag="ACG8MG_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0375183.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..3840
                     /locus_tag="ACG8MG_001121"
     CDS             3286..3840
                     /locus_tag="ACG8MG_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0375184.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4013..4450)
                     /locus_tag="ACG8MG_001122"
     CDS             complement(4013..4450)
                     /locus_tag="ACG8MG_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0375185.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            4498..5445
                     /locus_tag="ACG8MG_001123"
                     /pseudo
     CDS             4498..5445
                     /locus_tag="ACG8MG_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            5716..6151
                     /locus_tag="ACG8MG_001124"
                     /pseudo
     CDS             5716..6151
                     /locus_tag="ACG8MG_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            6167..7000
                     /locus_tag="ACG8MG_001125"
     CDS             6167..7000
                     /locus_tag="ACG8MG_001125"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0375186.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            7245..8270
                     /locus_tag="ACG8MG_001126"
     CDS             7245..8270
                     /locus_tag="ACG8MG_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0375187.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            8408..8533
                     /locus_tag="ACG8MG_001127"
     CDS             8408..8533
                     /locus_tag="ACG8MG_001127"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375188.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            8558..9673
                     /locus_tag="ACG8MG_001128"
                     /pseudo
     CDS             8558..9673
                     /locus_tag="ACG8MG_001128"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            9756..10652
                     /locus_tag="ACG8MG_001129"
                     /pseudo
     CDS             9756..10652
                     /locus_tag="ACG8MG_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            10668..11492
                     /locus_tag="ACG8MG_001130"
     CDS             10668..11492
                     /locus_tag="ACG8MG_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0375189.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            11524..12834
                     /locus_tag="ACG8MG_001131"
     CDS             11524..12834
                     /locus_tag="ACG8MG_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFQ0375190.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            12982..14241
                     /locus_tag="ACG8MG_001132"
     CDS             12982..14241
                     /locus_tag="ACG8MG_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0375191.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            14251..15123
                     /locus_tag="ACG8MG_001133"
     CDS             14251..15123
                     /locus_tag="ACG8MG_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0375192.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            15135..15965
                     /locus_tag="ACG8MG_001134"
     CDS             15135..15965
                     /locus_tag="ACG8MG_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0375193.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            16005..18188
                     /locus_tag="ACG8MG_001135"
     CDS             16005..18188
                     /locus_tag="ACG8MG_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0375194.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            18249..19469
                     /locus_tag="ACG8MG_001136"
     CDS             18249..19469
                     /locus_tag="ACG8MG_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0375195.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            19466..20926
                     /gene="gtfA"
                     /locus_tag="ACG8MG_001137"
     CDS             19466..20926
                     /gene="gtfA"
                     /locus_tag="ACG8MG_001137"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFQ0375196.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            21003..22109
                     /locus_tag="ACG8MG_001138"
                     /pseudo
     CDS             21003..22109
                     /locus_tag="ACG8MG_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            22431..22640
                     /locus_tag="ACG8MG_001139"
     CDS             22431..22640
                     /locus_tag="ACG8MG_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0375197.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            22633..22782
                     /locus_tag="ACG8MG_001140"
     CDS             22633..22782
                     /locus_tag="ACG8MG_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFQ0375198.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22936..23601
                     /locus_tag="ACG8MG_001141"
     CDS             22936..23601
                     /locus_tag="ACG8MG_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0375199.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="ACG8MG_001142"
     CDS             complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="ACG8MG_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFQ0375200.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            25304..25537
                     /locus_tag="ACG8MG_001143"
     CDS             25304..25537
                     /locus_tag="ACG8MG_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFQ0375201.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            25534..25941
                     /locus_tag="ACG8MG_001144"
     CDS             25534..25941
                     /locus_tag="ACG8MG_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0375202.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(27078..27596)
                     /locus_tag="ACG8MG_001145"
     CDS             complement(27078..27596)
                     /locus_tag="ACG8MG_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFQ0375203.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            27963..28211
                     /locus_tag="ACG8MG_001146"
     CDS             27963..28211
                     /locus_tag="ACG8MG_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375204.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         9843 a         4314 c         5245 g         9235 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc
     3181 ccttgatttt attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat
     3241 atacataatc attaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccggaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa
     3481 cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta
     3541 tatactgaag gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat
     3601 ataacaaggg atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat
     3661 gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga
     3721 gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac
     3781 attggtataa ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa
     3841 tttaaaaatt tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat
     3901 tttatttcat aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt
     3961 gttatataaa ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa
     4021 agagaatcaa tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact
     4081 caacgtttag cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt
     4141 tgaccttctg caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa
     4201 ttgccgtttc ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt
     4261 taatatctgt atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga
     4321 gcagactaca atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt
     4381 taatgatgat tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg
     4441 atgaaaacaa aagttgttca ccatctttta taggtttgat tctcattttt agatattatg
     4501 cccgcacgta ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt
     4561 tatcataata tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc
     4621 atgtatcacc tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt
     4681 gaagtacaag aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta
     4741 aaagttcctt ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt
     4801 tgccattatg gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat
     4861 gcaacacgat tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg
     4921 tatgtcacag accagatccc tacagagcat gccattttag gaattgatgc cattcaacta
     4981 cgcgtacaat attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa
     5041 gaatacgtca gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact
     5101 cagttctatc aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg
     5161 attggtagcg tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc
     5221 gacaaacaat tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt
     5281 gtatcacttt attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga
     5341 aacctagatg caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt
     5401 ttaccgcagt acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc
     5461 catgacgaag agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc
     5521 cattctaaag aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta
     5581 ataaaactac ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt
     5641 aaactaaaca tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat
     5701 aaaggggagt accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa
     5761 aaatgtcagt ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc
     5821 aggaaaatca tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga
     5881 cacctatgat agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga
     5941 gattgataat ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg
     6001 ctcaacgtta gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt
     6061 tgcaacacct agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg
     6121 ttctgtctgt cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc
     6181 ggctattgat aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg
     6241 aattgaggaa caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc
     6301 agaagatgtt aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt
     6361 acgtgtaaaa gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga
     6421 tacgcatctt tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt
     6481 aaatacaaat gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca
     6541 tgagaaaata gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga
     6601 acgacatcaa gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg
     6661 gatatataac aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat
     6721 tgttgttact ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa
     6781 acgagtgatg gaacaattat cgattattgg atttgatgat attacttttg cagaattaat
     6841 ggatcctgcg ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca
     6901 ccaactgatt ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg
     6961 agaattgatt tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt
     7021 atagttaaca ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc
     7081 gtcgcaaatt cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt
     7141 ttttaattat gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga
     7201 ttatatgtta gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg
     7261 atgttgcaaa acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc
     7321 cgacactttc agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc
     7381 attatgtcaa aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag
     7441 tttctgaagt agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag
     7501 tcgaagcaga acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt
     7561 tggagcaaga cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt
     7621 ctgtgattga agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta
     7681 ttgaagctga agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag
     7741 atcgattgta taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt
     7801 tagatagcca aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga
     7861 cgtggatgaa acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca
     7921 cacttgacgg aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc
     7981 cgactgctat catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa
     8041 aaaatggtct acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg
     8101 ctgaattttt aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac
     8161 tagcagttcg catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga
     8221 ttgtttctaa taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac
     8281 atccaaaaaa gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa
     8341 aacattaagt aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg
     8401 aattaagatg aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac
     8461 attactcgca gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag
     8521 ctatgcgagt tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga
     8581 aaaacaaaat cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt
     8641 taaattagaa gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag
     8701 cgaatcctat aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga
     8761 aaatgatatt gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga
     8821 cgaactatac gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt
     8881 atttgataat gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca
     8941 agctgcaatt gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg
     9001 aacagagact ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga
     9061 caatacaaaa tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga
     9121 tattgcactt gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt
     9181 aagtcgtttt caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg
     9241 atttttgaaa aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa
     9301 agttaatgca acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc
     9361 agaagaagtg aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc
     9421 ggaaacgggg gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc
     9481 agatacaatt gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata
     9541 caataaagat ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc
     9601 tggtgaagta actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt
     9661 gagcggaaat taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg
     9721 tatattaatg gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat
     9781 atcgtaaaga tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa
     9841 ttttattaaa tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag
     9901 ggttcggtga tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg
     9961 aagtttttca gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg
    10021 ttgtattatc cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt
    10081 atcggacaat ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc
    10141 gctggatgtt caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg
    10201 atggaccagc atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag
    10261 tttggagtgc tattggttat aacatggtgt tattactagc cggcttataa gaaattccta
    10321 aagattatta tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca
    10381 ttacgttacc attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg
    10441 ctttccaagt atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta
    10501 aatcacaatc attagcctat ttattctata aacattcatt tattttaaat gacaagggat
    10561 atggttcggc aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa
    10621 tgaagttgca gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat
    10681 ttttttcaaa tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac
    10741 cttttatttg gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac
    10801 ctacaatttt ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac
    10861 cattcatggc tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg
    10921 ttttattttc agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata
    10981 tccttttcag cttagtactt attcaaatga tgattcccgg ttccattttt attactccac
    11041 agtatttatt agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag
    11101 gggtagttag tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg
    11161 aattggagga agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta
    11221 tgttgccgct tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat
    11281 ttaaagattt aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc
    11341 cgggattagc aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt
    11401 cgttattagc aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg
    11461 aaggaattgc tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag
    11521 tacatgacaa aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta
    11581 ggcgatgcga tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat
    11641 gaaaaacgac taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa
    11701 aatcgtgctg atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt
    11761 gttatcaatg ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt
    11821 cctaaaaaat atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt
    11881 agaaccctac ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt
    11941 ccaaatgctt ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta
    12001 aaagcgacga gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa
    12061 ttgtttgagc atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt
    12121 gcaggcatta atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat
    12181 ccaacaattc gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt
    12241 gaattgttga aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac
    12301 catccatact ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg
    12361 gatgaatact tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac
    12421 attgtaaata atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg
    12481 gattctatta cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta
    12541 atcacaaact taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc
    12601 gtgcaaccaa cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat
    12661 atcaatgttc aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat
    12721 caagcagcgt atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat
    12781 ttagtggatg atttaatcga agcacatggg gactatttac ctgaatataa ttaaaaagaa
    12841 gctgaaagag taaggaattt tcaattcctt actcttttct tagttaaatt tagtaaaaat
    12901 attgacaaaa cacttagtaa acagttacat tagtgttaac aaaaagaaaa cgcattcaaa
    12961 ttgtataagg aggagcataa aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca
    13021 ctaggagcaa cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag
    13081 gtagaaatag agttttttaa tcaaaaaaaa gaaatgactc aaacgattca ggagattgct
    13141 aaagattttg aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt accgaatgct
    13201 ggtgaagtaa ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat taatctttat
    13261 cctcaaagta tagaattgaa agaatgggcg aaagctggtt atttagagga cttaaccgaa
    13321 gaaccgtatt tagagaatat caaaaatgga tatgcacaac gttttgctat tgaggatcgt
    13381 gtatatagca taccattaac cgctaatgtc tatggttttt actataacaa aactgctttt
    13441 gaggaaatgg ggattcaagc accagaaact tgggcagaat ttgaaaaaat agtagctgag
    13501 attaaagatc aaaacaaagt accgtttgcg attgcaggag cagaaggctg gactttaaat
    13561 ggttatcacc aactagctat agcgactgct gctggtggtg aagaggaagc gaacaatgtt
    13621 tggcgtttct cagaagttaa tggaattaat gctgaaagca aagaaatgca aaaagatttt
    13681 aatcgattag atttgttaag agaaccaaaa gctttgcaaa acaactggca aggtgccggt
    13741 tataacgata cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca
    13801 tgggcaatgc cgatgattca ttcacaaaat ccagattttg aagtaggaac ttttcctttt
    13861 ccagcagatg aagcaggtca aagtctaacg attggcgcag gagacctagc cttatcagtt
    13921 tctgcaactt ctcaacataa agaagaagcc aaacgttttg tagaatatat gacaacacct
    13981 gaagcgatgc agaaatatta tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc
    14041 gagaatactc aggattcacc attaagtggt ttaactgaac ttgcttttac tgatcgtcat
    14101 ttagtttggt tagccaaaga ttggaatagt gaaaatgatt tttatacctt aacaacaaat
    14161 tatttacaca atggcaatca aaaagccatg attaacgcat taaatgcttt ctttaatcca
    14221 atgaaagctg acggaaggta ggaaaataaa atgaaaataa aagaaatttt aaatcgttac
    14281 tgggcaatca tttttgtagc aattccaatt attttgcaga tgattttttt ctactttcca
    14341 atggttcaag gtgcttttta cagtttaact aattggactg gtctgactta taattttgat
    14401 tttgtgggtt tgaacaatta taagattctt ttttctgatc cgaagtttat caaatcgatt
    14461 ggcttcactt taattgtgac gctttgcatg attgctggac aaattggttt tggtattttg
    14521 attgcgcggg cgttgaacgc aaatattaaa gggaaaacat tctttagagc atggttcttt
    14581 tttccagcgg tcttatcggg cgtggttgtt tctttagtat ttaaacaaat ttttaactat
    14641 ggtttaccaa aaattggtag tatgttaggc attgaatttt tacaaaccag tcttttagga
    14701 acaacatggg gagcgatttt tgcagtaatt tttgttttac tttggcaagg aattgccatg
    14761 ccagtcatca ttttcttagc gggttttcaa agtattccac aagaaattat tgaagctgct
    14821 agaatggatg gggcgaatga taagcaaatt ttttggaata ttgaaatcac ctacttatta
    14881 ccaagtattt caatggtatt tattatggca ttaaaaggtg gcttaacagc atttgatcaa
    14941 atttttgctt taactggtgg tggaccaagc gatgccacaa catctttggg gttacttgtt
    15001 tataattatg cttttaaaaa caatcagttt ggttatgcga atgctattgc tttagtctta
    15061 tttatcttaa ttgggattgt ttcactggca caattaaaat tatcgaaaaa atttgaaatc
    15121 taggaggttt cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac ttgcttttat
    15181 tattaggggg cttcatgatt ttagttccct tagtttcaac gctattcggt tcatttaaaa
    15241 caacacccga tattatgaat aatttctttc gttttcctaa tccagcgact ttggaaaatt
    15301 tccgaagatt gttagcggat ggaattggtc attacttttg gaattcggca atcatcacaa
    15361 tcattgcagt agttgctgtg acagcaatta ttcctatggc tgcttattca ctagcaagaa
    15421 aaatgactcg tgggaaagca tacggattga tgtatttatt tttaatttta ggaatttttg
    15481 ttcctttcca agtaattatg attccgatta ccgttatgat gagtaaaatt ggtttagcaa
    15541 atatgtgggg attaatctta ctctatctca cttatgccgt accacagaca ctatttttat
    15601 atgttggttt tattaaacta aatgtgccta tcagtctgga tgaggctgct gaaattgatg
    15661 gggctagtca ctttacaact tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg
    15721 cgacgactat tattcttaat gctctatggt tttggaatga tttcatgcta ccattattaa
    15781 ttttaaatcg tgattctaat atgtggacac taccattgtt tcaatataat tatacgggac
    15841 aatattttaa cgactatggt ccaagttttg cttcctatac agtaggaatt gtcacgatta
    15901 cactagttta tcttgctttc caaagaagta ttatagctgg aatgagcaat ggagcagtga
    15961 aataaccaat aaattattct cattataaag gagttcataa acttatgatt atatttgaca
    16021 atcacacaca aacatttcat ttacaaaata aaaaaattag ctatctatta ggaatcgaag
    16081 aaaataatta tttaacgcat ctttattttg gaaaaaagat tacgcactat tctggtgggt
    16141 atcgctaccc ccgaacagat cgttccttct cacccaacaa tgcaaatgcg caagatcgtt
    16201 tattatcttt agacacatta ccattagaat ttccaggata tggacatggt gattttagag
    16261 aaccggcgca taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata
    16321 gctacgaaat tcaaaaagga aagcccagct tagcgggatt gccagcctta tatacggaag
    16381 atgatgatga agctgaaacg ctgattattc aattgaaaga cgcgattagt ggattgactt
    16441 tacagttgtt ctacacaatt tatcaaaatc acagtgtaat tattcgttct acgcaattag
    16501 taaataatag tgatgaaaca gtggagatta accgcttagc tagtcaatca attgattttc
    16561 caaatcgtcc actcgagtta attcatttaa atggtacctg ggcaagagaa cgtcaaatga
    16621 ctagagaaaa aattcataca gggataaaag tattagatag taaacgcgga tcaagcagcc
    16681 atcaacaaaa tccgtttgtt gctttagtag atgccaaaac agatgaattt caaggagagg
    16741 catatggatt taatttagtt tatagcggta atcacgaaac ggtgattcaa aaagatccat
    16801 ttgaccaaac acgggtcatt atgggaatta attcatttaa ttttggttgg caactaaaaa
    16861 ctggcgactc atttcaaacg ccagaagtcg tgatggttta tagtaatgaa ggtctgaata
    16921 cgttatcaca gacgtatcat gaattgtata atcaccgttt aattcgtgga aactatcgtt
    16981 taaaagaacg tcccacgtta atcaacaact gggaagcaac gtattttgat tttaacgaag
    17041 aaaaaatcat gaatattgtc gatgatgcga agaacttggg cattgaaatg tttgttttag
    17101 atgatggttg gtttggcggt cgtgaaaatg acttcacttc attaggtgat tgggttgaaa
    17161 cagaaggcaa acttgcttgt ggtttggaaa atttagccaa gcaagtgcat gataacggga
    17221 tgaaatttgg gatttggttt gaaccggaaa tgatttctga agatagtgaa ctatttcgag
    17281 cacatccaga ttgggcattg ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat
    17341 atgttcttga tttttcaaga caagatgttc gtgataatat ctatcaacaa atgacggcga
    17401 tttttgatcg tgtaccgatt gattatgtga agtgggatat gaatcgaaat atgacagaag
    17461 tctattctgt cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag
    17521 ggttatatga atttttagaa aagttaacaa cgaattatcc aactatttta tttgaaagtt
    17581 gttcgggtgg aggcggtcgt tttgatgcag gtttcttgta ttacatgcca caaacttgga
    17641 cgagcgacaa taccgatgcc gtggctcgtc tgaaaattca atatggaacc agcttagtgt
    17701 atccaatttc aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa acaggaagag
    17761 aaacgagttt aactattcgt ggcaatgctg cgatggctgg tgtttttggt tatgagctag
    17821 atctaacgca attaacttct gaagaaaaag cagaaattaa agaacaagtt gccttttata
    17881 aaaagcatcg ccaacttttg caatatggaa ctttttatcg cttggaaagt ccgtttgaag
    17941 gcaatgatac tgcatggatg tttgtctcaa aagaccaaac agaagccatg gtcttttatt
    18001 tccgagtatt agcagaagct gcgcatccat tagtcacatt gaaattagca ggattagcag
    18061 aagaagcaac ttatcaaatg gaagatgtaa catttgctgg cgatgaattg atgaatctcg
    18121 gtttttatat tgactcagaa ctacatggtg attatgcgac acaacgcttt tatttgaaac
    18181 agatatagta gcttgaattt acaatataag tgcaacacca aaagagactc attccaaacg
    18241 aagaaaatat gttttattta cacgcattta ctaaaaagca gcctgatttg aattgggaaa
    18301 atgaagaagt acgtgaagaa atctatgcaa tgattaacta ttggttagat aaaggactag
    18361 gcggatttag aatcgatgcg atattaaaca tcaaaaaaaa actagagtat ggcatctttg
    18421 aatctgatgg agaagatggg ttagccttca ttgggccctg gattcttaat cagtcgggca
    18481 ttgaagtgtg gttaaaagaa atgagagagc ggacatttaa accacataat agtatgacgg
    18541 tagcagaagc cgatgttcct aatgaacgtt tagatgaata tattggtgaa gatggctatt
    18601 attcgatggt ttttgatttt agttatacgg atattgatgt acctgaaact ggtgaatggt
    18661 ttaaagatag ccaatggaca tggacggata tgcgtaccaa tatttttacg aatcaattag
    18721 ttacgcaaga taaaggttgg ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa
    18781 ttaataaata tattccagaa gaatggatca atgattatag taaaaaaatg ttggcgacgc
    18841 tttttatgtt actaagaggt acccctttta tttatcaagg acaagaacta ggaatgacca
    18901 acatagaaat ggcatcatta gaagattttg atgatgtagc tacgcattct cagtataaac
    18961 gggcattaga atatggattg gcaccagagc aagcattgaa agctgtcaat aaacgaagtc
    19021 gggataattc tcgcacacct atgcaatgga atacgcaaaa aaatgccaga ttctcaactt
    19081 ctgataatgt atggttaaaa gtaaatccta attatccaga actcaatgca gaagctcagt
    19141 taaccaatca acagtcggta ttcaattatt atcatcaatt aattaaatta agaaaaaata
    19201 gtgtatacaa agacgtgcta atctacggac aatttctacc agtgaaagat aaaaatgaac
    19261 atattttgct ttatgaacgc caattagaag ataaaatcgt attagttatc ttaaacgtta
    19321 cagcaacaga acaagaatat tatgtagatg aacagtacca acaagtatta atcaataatt
    19381 atgaagatgt tttattagat caaagtcgaa aattgcgttt acgaccgttt gaaagtattg
    19441 tattagctaa ttatggagga caattatgaa aattaaaaac gaagcaatgc tcatcaccta
    19501 tgcagatagt ttaggaaaaa acatgggcga attaaaacaa gtcttagaca aacatttaca
    19561 aggtgttatt ggtggtgtac atctgttgcc tttttttcca tcgacaggtg atcgaggctt
    19621 tgcgccgagt gattatacga cagttgatcc agctcttgga acatgggaag atgtggaagc
    19681 attaggtgaa gattattatt tgatgtttga ttttatgatt aatcacattt ctcgtgaatc
    19741 aaaatttttc caagatttca aaaaaaacca tgagcagtca ccatacaaag aaatgtttat
    19801 ccgtattcat gaatttttcc cagaaaatcg accaacacaa gaagatattg atttaatcta
    19861 caagcgtaaa gacaaagcgc cgtttcaaac ggtagaattt gcggatggta cgacagaaga
    19921 ggtttggaat acctttgggg aagaacaaat tgatttagat gtgaccaaag aagttgtgaa
    19981 agaatttatt cgagaaacca tcaaagatat ggcgaaccat ggctgttctt taattcgatt
    20041 agatgcgttt gcttatgcga ttaaaaaatt agataccaat gatttcttcg tggaaccaga
    20101 tatttgggaa cttttagacg aagtgcgtca agaagcagcg aaatatgacg tcgaattatt
    20161 accagaaatc catgagcatt attccattca aatgaaaatt gccaatcatg attattatat
    20221 ctatgacttc gccttgccaa tggtcactct gtattcactt tatagtggaa aatcagaacg
    20281 tttagccaat tggttgaaaa tgagtccgat gaagcagttt acaacattgg atacgcatga
    20341 tggaattggt gttgtggacg ctcgtgattt attaacagat gaagaattgg attacacctc
    20401 tgaggaattg tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa
    20461 taatttagat atttatcaaa ttaacagcac ctattatagt gcattaggcg ataatgaccg
    20521 tagctatctg ttagcacgtg cgattcaagt atttgcgcca gggattccac aaatttatta
    20581 cgttgggtta ttagcaggaa aaaatgatat tgaactgttg gaagcaacga aagaagggcg
    20641 taatattaat cgtcattact atgatttagc cgaaattgaa gaagaagtgc aacggccagt
    20701 gattcaacaa ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga
    20761 tggaacgatt gaagtagcaa cgccatcaga atcagaaata gtgattacac gtaaaaataa
    20821 aaacggtagt gatagtgcaa cattaacggc taacttggat acgaaagaat ttatcattga
    20881 gtctaaagat ataaaaattt tcactagtgt tgcattaaaa ttttaaccta ttccatagaa
    20941 caaaaaatcc tttatactag cacttggatg acttatccaa gaccaataaa aaggactcat
    21001 acatggataa gtataaaaca aatacagcat ttaataaatg gttttctgcc ataaaattag
    21061 gaaaactttc tgtcaactcc caaaaatgta tttttgatta caattcgtat cgtaaaaaac
    21121 tgtcttttga agctgtgctc aagctctttc tctacgcagt taatgaagaa aaagaaagtt
    21181 tgcgtgattt gagtacagct cttatcgacc gatcgctcca actagaaacc gatgtgtcat
    21241 cgattagtca tacccaattg tctcgagcat ttaatgcact tgaacctaaa gtgttagaag
    21301 aaatttttca acaactgtta gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc
    21361 atctgtatct gattgattcc agtacgtttt cgcttagttt aaaacgtcat aagtgggcga
    21421 ctttccgtca aacaaaatca ggtgtcaagt tacacttaaa ctattgttat atggacgata
    21481 ctacgatgta tccaactgat tttaccttaa caaatgccca tgaacatgat ctcaatcaac
    21541 taccagtatt ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg tattaggact
    21601 ttgaaaagat ggatcagatg cattgggatg gctatttctt cgtcactcgg attaaaaaaa
    21661 acaccaaagt tcacgtgata gatactctgg aaacatcgcc agaaacagaa attctacgag
    21721 acgagctggt tcgtttagga tctaaaacct atctaaccgc caatttccga ctagtcactg
    21781 ttcaagataa gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa
    21841 aagaaatctc tgatatgtat cacgctcgtt ggcaaatcga attgtttttc aagcacatca
    21901 aacaacacat gaccattaaa accttctttt ctcaaagtga aaaaggcgtg cagaatcaat
    21961 tgattttaac gatgatttcc gctctcctca cgtttctcat caaattggag acgaaaacgg
    22021 aaaaatcagt gttccaaatc aaacgattct ttcgttattt actgtttcag ccctttgaat
    22081 gttgtctaga aaaattaatt cccacatgag aaaaaagaaa tttggctcgt tacttttggt
    22141 tttcagttga ataacagata aaaaattttc tggaaaaagt catcctctgc gtcagcacag
    22201 ttttttttga cttattttca gaggaaatag ttttcagaat attctaaaat gttttatgca
    22261 acactagtga tattttttag aattagattt ctaatgtgca ttttttgagt attaaggagc
    22321 aagtatacaa agatttataa agctgattaa atttgaataa aaatatgcag gaacgctact
    22381 atttttagta gtgttctttt ttatatttga ataaaaaatg tggtggttaa atggtggtta
    22441 taacaaaaaa tgaattaatg aaattaggtt atgggaagta tcagtctgag agaatcatac
    22501 gacaggcaaa agcaattatg gtttcaaaaa gctataccta ttacgaaaat agaagattag
    22561 gactagtacc tgttgaatct gtagaagaag ttttaggtat gaagatagag gtagaggagg
    22621 attttcagaa tcatggttaa ttttaaaaaa gataaaaatg ggaaatatca ttttactgct
    22681 agtttgggat tcgacaaacg aacagggaaa agagtacaaa aaaacgtaga ggggttaaaa
    22741 cagttaaaga agaaaaaaag cgtataccga acttgtttct aattatggaa aagaagcaga
    22801 aacttctcaa agtactatgt tgtttgagga gtttttttat aaattattcc taccttatta
    22861 tcaagggaaa gtagaagaaa gaacatataa aaatcggtta tctacaatca aaaaaacttt
    22921 tcttatttct ttaaaatgga gttaagagaa atacgtccaa ttgacattca aaagtggcaa
    22981 aataatttat tgagcaaata taaaaattct tatgtaagaa acatgtatgg gatttttcag
    23041 atgtcattag atcgagcagt tgttttggga ctacttgaaa aaaatcctgc aaaagttatt
    23101 ggaaatgtga agaaaaggaa aactacagtt gatttttgga caaaagaaga atttacaaaa
    23161 gttatttcaa ctttttatat tgatgatttg taccaacatt ttggttttat ttgtatgtgg
    23221 ttacttttta tgaccgttat gcgtttggga gaagccactg cattaacttg gaaagatatt
    23281 gattttgaaa ataaaaactt gcgtgtgaat aaaacactct attataagag agccaaaacc
    23341 tatgagtttg tagaacccga aacggcttct agtattcgta caatcgaact agacgacaat
    23401 acaattacct ttttaaaagc ttggaaggag cgtcaagttt caataggagg catgtcgttt
    23461 gtactttctt ataatggaac acctactcag aaatatacaa tccgtcatat tatgagaaga
    23521 catgcaacga tcgttggtgt tcatataatc cgcatacacg gattaaggca tagtcatgct
    23581 tctttgctta tatcacaata aaaagatgca cttcgaatta aagaaaggtt aaaacatgct
    23641 gatgtacaaa cgactttagg aacttatggt catttgtatc taaattcatt gagagagata
    23701 acaaatgatc ccacagatta tatccaaact aatttttcta aaatcaaacc gacacctatt
    23761 tacgataata aaaaataaat gataaacaaa agtaggatca caccaaaaga aacacactag
    23821 aaaaactata atacaggctt ttctagcgtg ttgtttctta tttccgccta tttatacatt
    23881 atactcataa agcatttaat gcatcgatcg tagccgcatt gtctatatgg gtatataaag
    23941 ttgttgtatt ggtacttgaa tgtcccaatt gttgggcagt taataccaat gaatttgtct
    24001 gttggtagag tctagtagct aatgtatgac gcagtttgtg agggctgact tgaattctaa
    24061 agccttttga atattttgct accatccgtt cgattgcaat accggatata cgcttggctt
    24121 ctccttggta aattgctaga aataaagctt tttcgttttc tgttacttta tatcgttctt
    24181 tccgaatagc tagataatta gctaaatatt ccattgcgaa tgctgctata ttgacagcat
    24241 ctcttttatt tccttttcta gttactacca cattattctt atctaaagat aggtcatcta
    24301 aattaatatt tataacctcg gagagtctca atccagatcc taaaatcagt gcattgatcg
    24361 ccaggtctct ttctacatct cgttgataat agatcaaagc tcgtttactc aaagtatttt
    24421 tatattcatt ggcaatataa tctagataat tttgtgtatc cttatctaga aataattttt
    24481 tctcaatggc attggcacgt gcagctaacg tagttttatc tgtgatggtt gctacttgat
    24541 ccataggatt attgtctaag tatggttttc ctagaggatt attggaactt ttcgaaagat
    24601 atttaaatag aacctttaat gccgtgatat ttctattgat agtagaagta gatagaccta
    24661 agttattatt atctttgagc ttagtttttg ttgtctcttt ttttacattt tcttttgtgc
    24721 gacgtttcaa atgagatttg tacaagttta ggtcataggc agttaactgt cttatcgagg
    24781 tgatagaagt atcaggaacg atagactcac tcagaagcca ttcataaaag agcttataat
    24841 tttttagata ttctaaagtg gttcgaggtg attgatcttt atcctcacga tcttgaacaa
    24901 aatcttttat ataatcgggc atatgaagta tgattgtatc tattttttga tatatttctg
    24961 tatttttcat gtcattcatc ctttcaataa gtaggggaga agagtatctc tataattgga
    25021 ctataccaat tatagaatgt tttgtcaatt ggtatgctct aaaatttatt aatttgtttc
    25081 aaaaaaagca tataaacagg tatacgttta ataatttaaa ataaatggta tacataaata
    25141 gggaaggaaa tagaagggag caatctaaat tttttgaatt ttcttaatta attatataga
    25201 aagaacaatc tttctataga gttagacatg agagggacta tgcttgattt aaaatcaaca
    25261 agcgtatata ctgtatatac aaagaaaaag gagggagaag actatggcaa ttaaagaaag
    25321 aaaatttagg gaagtgggga actcagtagt attaactatt tcaaaggagt ttcttaaaga
    25381 aaatgggtta aatcccggag atacggtttt tttagatgaa gaaaaacttc gagcggcaat
    25441 tacaaaaaaa caagccttat ctgatgaaat tgatttagca atatctcaag cattaactga
    25501 atatgatact acttttagag agcttgtcga taaatgagta ttcattacct agaagccaaa
    25561 gatattataa aaatgaatgt aatccaaata aaaaaatact ctcctaatga accccttggt
    25621 gtaaaggatg cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc agcttttggt
    25681 gaagaggttt atccttcgat ttatgaaaaa gcagctatcc tacttataca attgataaag
    25741 aaacatcctt ttcacaatgc aaataaacgt actgctttct tagctacttt tgtctttttg
    25801 aaaatcaatg gacagctttt agctattgat caaaaagatg ctgtaaattt agcggtatat
    25861 attgctacgt atgataagga tttcgatcaa ctaaaatatg aagtgatcaa cactataaag
    25921 acacatacgc agcccattta aggagttact gtatgtgtct ttatagggtt attagctgct
    25981 atagcgaaat aattgaagta aattacaaat agctataatt caaatccaac tatatagaaa
    26041 acgtaaattt tctatatagt tggattaaga taacgactat ttattttatc atatttttac
    26101 tataccctat acttttactt ggtatattta tgaataatcg caaatttacc cctatactac
    26161 tagtttgtaa gttagattaa taaaaatact aaagttattt gttcttttat taaatacacc
    26221 tacaacttat aaattgatgg tatttagata acacgaaaga ataaaaataa cagctgatag
    26281 taaagtttgt tgacgattgc tactcaatcg aaagtgagtt agtgcgtatg aacttgcttc
    26341 tgaaaactta tgaaatgaaa gaccttttac atcttgcaga aatcgttact gggattaaac
    26401 taagcaataa aaactaatgt tcccctcatt ctttgaactt ctactatttg atttatttat
    26461 tctgataaat gacgatttca ccttcaggat cttcaaatcc cgtatagaat ttatttaaga
    26521 tctcttcagt atcagtaata ggataggcga taggccacat accctttgat ggttagaata
    26581 aactgctcaa aatctgtatc gtatgttggc tttagcatat taacaaaggc tatctccaaa
    26641 ttataaacca taagcgttga atcgttctta acaatttcaa ttcttcctaa cccaaagtca
    26701 gaagatataa ttaccggact tgccttaccc ttcatgtggg ttgttgagat tccatattta
    26761 aagggaattg tcggtactat cggcggtctg aaaaacatcc caaaaataaa aaaatctttc
    26821 ccctgctagt ctcctaagtc cctttgtagc ggatatggca ccataatctc attttatcga
    26881 agtgagtcat ggtttatctg ttggccaacc aaaattgata caatctaacg atgcaccctc
    26941 tacggcattc taggggagtg cacttgcatt cacccaataa aaaccggcag ttagcaaaga
    27001 acttatgtag tcgtatgttc ttactgttaa catacaagag gtctcctttc cacaattgct
    27061 tagcagtaaa cgggaattta caaagctaat ctcttccatt ttcttatctt tgttctaaaa
    27121 gtcccaaatg gagcaactgt atttacatga ataaatttat atacttccca tacagccgtt
    27181 ttggttgcct cgtctgccca ttttcttttg tgtgcagtga atagatcttc atcactcatt
    27241 gaatcaatca tcatgttaat atcatcaata ttacgattga gaatttcctc taattctttc
    27301 aatggtagat gtgcataggt atttgtaaac cacttatata actctcctaa ctgattccat
    27361 ttaaattgat cagaaggcgt tttaacagat aatccatttc tttcatcatt ttcccatttt
    27421 agaattaaat tcgtccaacc aacttgatat gctaaatttt cagcaggtgt tctatccact
    27481 tcctcaactc ttttatcctt caattcttca ggaatagttt caaattctaa aatatattta
    27541 gtataagtcc tatttatttc tttttttaat tcttctttat ttttatatgt tctcattata
    27601 ttacctccat aaattcctaa ttgtctcctt taattcatct ataatttcat ccacgatatt
    27661 ttaatccgtt ctacaatgca tatctgatat gcattgactg tctaactcat atgtgaatga
    27721 tttataaaat atcccaacgt ctcatctaaa gaaatgttga tcgtcagcaa ggagctaaaa
    27781 aatacgaata tatttctttt gtagtaaaat cggactggtt tgcatatcat tttcttcgta
    27841 aatatcaaaa tcatgttaac tactatcaat tactaataaa aactaccact ttaagcgtta
    27901 agaactgtta agtttagtta agtgaggata gttcacaaaa tccattgtta aacttcacga
    27961 aagtggtaaa actcaaaatg ctttatatag gattacagca tcgctctttc ttcgattgcg
    28021 cgttgggtaa aaaatattat gaagtaaaaa ttgacgatga cacgttcctt acggctcatc
    28081 agatgaaaca gctacaaaaa cgaaatgatc aactcgaaga aaatttaatt ctaaaaaagc
    28141 gattgccata ttcacgcctc gctcgacgaa ctattaaagg tcaagattta cctgaagatc
    28201 atccttttta ggaaattgaa gctttttatg ttgcacaatg catttataat actacaatga
    28261 tgttttcacc agatattctt ctagggtgcg gtgtaatgaa acagatacac ttgcaaaaga
    28321 aaattcaccg cgaatttact aaaattatga ataattatgt taatcttccg aaattttttt
    28381 ccaaaattaa gggatcaagc tggtgttata gattgtttaa ggttggcaaa aaaataactt
    28441 ctttttaaac aattatagaa aaccgatact ctgttaagtt ggttgacacg gtgtcgattt
    28501 ttatgacttt cttattctga aacttcacta acggacagta cctaaaaagt ctatagtatg
    28561 gacgtttacg agtactattt tatcaaaaat gctgctcatt tcaacttata tgttttagtg
    28621 aactaccccc acttagc
//
LOCUS       DAZKNH010000028        28599 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000028 DAZKNH010000000
VERSION     DAZKNH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.028"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            235..1875
                     /locus_tag="ACG8MG_001147"
     CDS             235..1875
                     /locus_tag="ACG8MG_001147"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFQ0375205.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2029..3351
                     /locus_tag="ACG8MG_001148"
     CDS             2029..3351
                     /locus_tag="ACG8MG_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFQ0375206.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3413..3844)
                     /locus_tag="ACG8MG_001149"
     CDS             complement(3413..3844)
                     /locus_tag="ACG8MG_001149"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0375207.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3908..4744)
                     /locus_tag="ACG8MG_001150"
     CDS             complement(3908..4744)
                     /locus_tag="ACG8MG_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFQ0375208.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4934..5236)
                     /locus_tag="ACG8MG_001151"
     CDS             complement(4934..5236)
                     /locus_tag="ACG8MG_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFQ0375209.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5262..5693)
                     /gene="ruvX"
                     /locus_tag="ACG8MG_001152"
     CDS             complement(5262..5693)
                     /gene="ruvX"
                     /locus_tag="ACG8MG_001152"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFQ0375210.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5690..5956)
                     /locus_tag="ACG8MG_001153"
     CDS             complement(5690..5956)
                     /locus_tag="ACG8MG_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFQ0375211.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6210..6935)
                     /locus_tag="ACG8MG_001154"
     CDS             complement(6210..6935)
                     /locus_tag="ACG8MG_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0375212.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7301..7371)
                     /locus_tag="ACG8MG_001155"
     tRNA            complement(7301..7371)
                     /locus_tag="ACG8MG_001155"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7337..7339),aa:Gly,seq:ccc)
     gene            7488..8240
                     /locus_tag="ACG8MG_001156"
     CDS             7488..8240
                     /locus_tag="ACG8MG_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0375213.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8256..8735
                     /locus_tag="ACG8MG_001157"
     CDS             8256..8735
                     /locus_tag="ACG8MG_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0375214.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8986..10566)
                     /locus_tag="ACG8MG_001158"
     CDS             complement(8986..10566)
                     /locus_tag="ACG8MG_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFQ0375215.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10559..11302)
                     /locus_tag="ACG8MG_001159"
     CDS             complement(10559..11302)
                     /locus_tag="ACG8MG_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375216.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11478..12254)
                     /locus_tag="ACG8MG_001160"
     CDS             complement(11478..12254)
                     /locus_tag="ACG8MG_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0375217.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12502..13338)
                     /locus_tag="ACG8MG_001161"
     CDS             complement(12502..13338)
                     /locus_tag="ACG8MG_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFQ0375218.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13349..14278)
                     /locus_tag="ACG8MG_001162"
     CDS             complement(13349..14278)
                     /locus_tag="ACG8MG_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFQ0375219.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14470..14823
                     /locus_tag="ACG8MG_001163"
     CDS             14470..14823
                     /locus_tag="ACG8MG_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0375220.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14897..15553)
                     /locus_tag="ACG8MG_001164"
     CDS             complement(14897..15553)
                     /locus_tag="ACG8MG_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0375221.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15779..17713
                     /locus_tag="ACG8MG_001165"
     CDS             15779..17713
                     /locus_tag="ACG8MG_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0375222.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17799..19022)
                     /locus_tag="ACG8MG_001166"
     CDS             complement(17799..19022)
                     /locus_tag="ACG8MG_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0375223.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19016..20887)
                     /locus_tag="ACG8MG_001167"
     CDS             complement(19016..20887)
                     /locus_tag="ACG8MG_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFQ0375224.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(20908..22026)
                     /locus_tag="ACG8MG_001168"
     CDS             complement(20908..22026)
                     /locus_tag="ACG8MG_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0375225.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22109..22942)
                     /locus_tag="ACG8MG_001169"
     CDS             complement(22109..22942)
                     /locus_tag="ACG8MG_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0375226.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22935..23852)
                     /locus_tag="ACG8MG_001170"
     CDS             complement(22935..23852)
                     /locus_tag="ACG8MG_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0375227.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24079..25203)
                     /locus_tag="ACG8MG_001171"
     CDS             complement(24079..25203)
                     /locus_tag="ACG8MG_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0375228.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25386..26294
                     /locus_tag="ACG8MG_001172"
     CDS             25386..26294
                     /locus_tag="ACG8MG_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0375229.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26435..28048
                     /locus_tag="ACG8MG_001173"
     CDS             26435..28048
                     /locus_tag="ACG8MG_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0375230.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8891 a         5621 c         4868 g         9219 t
ORIGIN      
        1 ctatttatct atctaacata tcctttattc ttctactttt ctttctttag catataagag
       61 atacgcatag taatcaagca aggattgttg atataaaaag aacaaatacc gctattttga
      121 tttcttttga ctctattact catataaata aaatttatta tacctgctct ttttgactat
      181 atcctcatct gactcctata cttcgtttgt attaaaactt aggaggaatc attcatgctt
      241 acagggctaa acttattaaa taatcctttt ctaaataaag gaacagcttt tacaaaagaa
      301 gaacgggcaa aatacggaat cactggtatg cttccaagta ctgtccaaac attggaacaa
      361 caatctgtcc aagcttacgg tcaatactta agcaaacaga ccgatttgga aaaacgtatc
      421 ttcttaatga atctattcaa tacgaaccgg acattgtttt ataaattgat gggcgaacat
      481 ttagttgaat tcatgcctgt cgtttatgat cctgtcgttg cggattcaat cgaacaatac
      541 aacgaaattt tcttgaaacc acaagatgct gtatttcttt caatcgacga accagaaaat
      601 atcaaagcaa gtttgaaaaa cgcagcagat ggacgcgaca ttcgtttgat cgttgtgaca
      661 gatgcagaag gaatcttagg tatgggtgac tggggtgtga acggtgttga catcgctatc
      721 ggtaaattga tggtctacac tgccgcggct ggtatcaatc ctgcacaagt cttgcctgta
      781 tcaatcgacg ctggtactaa taacgaagaa ttattgaata atccattata cttagggaat
      841 cgtcatgctc gtgtagaagg cgaaacttac tacgaattca ttgatcaatt cgttcaatca
      901 gctactgaat tattcccaga attgttatta cattgggaag attttggacg aggaaatgct
      961 gcaacgatct tagaaaaata cgaagataaa atcactactt tcaatgatga tatccaaggc
     1021 acaggaatcg ttgtactagc tggtgtatta ggtggattga atatatcagg tgagcaattg
     1081 aaagatcaaa cgatcttgac atttggtgct ggtactgctg gtgtaggtat cgcaaacatc
     1141 cttttagatg aaatgatccg tcaaggtgta cctgaagaag aagcacgcca acatttctat
     1201 caagtagata aacagggttt attgtttgaa gatacaaaag gattgactcc tggtcaaatc
     1261 ccattcgctc gtaaacgctc agaattcact aatgctgatg aattgacaaa cttagaagca
     1321 gttgtaaaag caatccatcc aacgatcatg atcggtactt ctacccaacc tggagcattc
     1381 actgaagaaa tcgtgaaaga aatggcggct cataccccac gtccaatcat catgcctttg
     1441 tctaatccaa caaaattggc agaagcaaaa gcaaaagatt tgattgaatg gacggacgga
     1501 aaagctttag ttggaacagg tatccctgct gctgatgtgg aatataacgg tgtaacttac
     1561 caaatcggtc aagcaaataa tgcgttgatg tacccaggtt tagggcttgg tttgattgct
     1621 tcaacagcta cacgagtaaa tgctgaaatc atttctcaag ctagtcgagc attaggcgga
     1681 atcgttgatg taacaaaacc aggagcagct atcttgcctc cagtagcaaa aatcactgaa
     1741 ttctctcaaa cgattgctga aactgtcgca aaatcagttg ttgcacaaaa attgaatcgt
     1801 gaagaaatca cggatatcaa agaagctgtt gaatctgcta aatgggttcc tgaatataaa
     1861 tctttagaag attaagattg ttatagtaaa agaacataca gaaactttgt atgacgtcta
     1921 aaaagaaaaa agcgactgcg gcaaaacagc agccagccaa ctactgctct tttgccgctt
     1981 ctctttggct tttttcatac gatacagaat aagaaaggat ctgtaaaaat ggcacaaaaa
     2041 acaacaaccg caactgcagt tgaggaaaag aaaaaagaag ggttttggtc aacaaaaatt
     2101 accggcgtaa gtttgcccct ttatttgatc atggtccttg tcctgatcat tgcaatggct
     2161 ttaggaaaat taccgacgaa tatgattggc ccaatcgctg ttttggtcat tttcggtaat
     2221 ttatttcatt ttatcggaac aaaaatccct atagtcaaaa gttatttagg tggtggctcc
     2281 gtttttgcga tctttgcctc tgccgcgatt gctacatttg ggatcttacc tgagtatgta
     2341 gtaaaatcca cagaaaactt tgtcagcaat atgggctttt tagactttta tattgctgcc
     2401 ttgattactg gtagcatcct tggtatgaac cgtaatctat tgatgaaagc ttctgttcgt
     2461 tttattcccg ttgcacttat ttcaatggtc gtctcctttt ttgccgtagg actcgtaggt
     2521 atgcttatcg gaaatggttt tgcaaattcc gttttatata tctcattgcc cgcaatggct
     2581 ggtggtgttg gagccggagc tgtaccttta tcaacgattt atgcaaatgt tttaggtacg
     2641 gacgcttctt ctatcatttc ccgcctgatt cctgcgacag caatgacaaa tgtcctagct
     2701 atcattggtg cagcgttagt tgctcgtgcg ggacaatcaa tgccaaaatt caacggtaat
     2761 ggtaaattaa tggaaaaagg cgaccttggc gatggaaaac ctagaaatgt aaaaccttcc
     2821 attccacaat taggtgtcgg tttgatggta tcattgactt tcttcatctt aggacaaatc
     2881 tgtaactatt ttgttcctaa agtccatgct tacgcattca tgatcattat cgttgttatc
     2941 tgtaaaatcg caaacctatt accagaatat tacgaagatg cagctatcat gttcaacaac
     3001 ttgattgtca aaaacttgac agcagctgtt cttgccggta tcggtattgc gttattgaat
     3061 ctaaatgtat tggcttctgc tttgacatgg caatttgttg tcttatgttt gacaagtgtt
     3121 atcgcgattt cagtagtttc aggatttgtc ggtcgtttgt ttggtttata tccaatcgaa
     3181 tcaactatta ctgctggctt atgcaacaac tcgatgggtg gtacaggaaa tgtagctgtc
     3241 ttatctgcag ctaatcggat ggaacttatt gcttttgctc aaatgggcaa tcggcttggt
     3301 ggtgccatcg ttttgatcat ctcaggtttc ttgatgcaat tactttctta gtcaaaaaaa
     3361 atacctgatc atcaagtatc ggcttgatga ccaggtattt tttgataaaa atttacagga
     3421 ttttcaaaaa tcttttttgc attttatcgg aatgataacc gatgcgttca taaaatcggt
     3481 gtgcttctac tctgtattct gcagaattaa gtcttattgc tgatattcct cgtcttttcg
     3541 cttcttctac gccagtcatc aaggcctttc cgattcctct tttttcagca tgtttagaca
     3601 cagcaagtgc cataatattg aacatcggat cgctgaaaat cgattcatag gactccgctt
     3661 gtacatagcc agctgctttt ttagaagtag gatcttcata gaccaagaaa aaattttttt
     3721 ctggctcctt caacagtttc ttcatttgtc tcaccgtaac ggttaatggt acatcatatc
     3781 ctaattgttc cgcattgatt tctcgtagtg cttctgcatc tcctaaacgc atttcccgca
     3841 acatccaaat caccagccaa tttttatttt tttaaatgaa tttttttatc acagtatact
     3901 ccttttttca cttaaaaaaa gacttttcag tgattttgac ttgcttgaat ccagatcctt
     3961 ctaatgtttg caagatgacg aaagcattgt aaagattgat caaaagaacc acgatgacaa
     4021 tcgcttgaaa aataaaggtc actttttttg gagacaaaac cctgctaaat cttgctcccc
     4081 acaaacctcc aataacagca gcggggataa cataaaacaa catagataaa tcaaagcgca
     4141 taaagctact tgttaaagca atcgttacca attttgccag ttgagaaaag aaaatcgtag
     4201 atagagaata tagtgttgct tcttttattg gcaatgcaaa catcagcatc aacaaagaaa
     4261 cattgactgg tccaccacca atacctaaaa aactagctaa aaagcccaga acccccccac
     4321 atataaggta ccaagtccat gacgtcaaat gaaattttgg ctgatgatgc ttggtgtaga
     4381 agaacgcaaa aaccaaggtc aataccgtca aaaaaatttg gatcagctgc acatgcttct
     4441 cgttttcaaa taactgtaaa aaaacttcga atacaatgtt gccgactact cctccaacaa
     4501 cagctccact agatacccaa ccgacaatct gccaattcag tgatttcccg cttgatactt
     4561 gtcgcacagt cgaaacaatg gacatcgtga ataccgcaac tgttgagtaa aaagaaatcc
     4621 ctgcaacaga atgggcgccg ataagatcaa gaatcggttt gatcaacacg cctccaccca
     4681 tcccggaaat agcgccaact gtgttcgcca gaacgatcac cacaaaataa atcagaccaa
     4741 tcatcttgta tttcctttct cttgtttata tcttttagcg attgttactc ttttctttta
     4801 ttatacagta aaagaaacaa acgaggcttc gaaagctttt ctacttttta aggaaattta
     4861 gtgctaaacg aaaaaaccgg gctctacttt tcagagagtc cagcttttct tacaccttat
     4921 cgattctttt gttctattct tcttccgcca tgaatgtatt gaatacttct tcgatcatat
     4981 cccattctgc gtctgtttcg atttgttcta actcgccttc tgttccatct tcgttttcga
     5041 tgtatgcata agcttgtaat tctacatctt catcttctga aacaccagct ggatataaaa
     5101 gaacatagtt acgtccaaat tcttcttgtc cgtcaactgt tagcaagatt tcgtataatg
     5161 tttcatttcc ttggtcatcc accaatgtga tgtgttcgtg tccttcgtgt tcgtgggaat
     5221 ggttatgttc tgtcataaga ttacctcttt tctttttatt attaaaataa ttgcttacct
     5281 gagccgtcta aataattttg taaaatcatc acagctgcta atttatcgat gacttttttt
     5341 cgtttcgctc ttgaagtatt tgcctgctct actagcatac gttctgcctg aacagtggtt
     5401 agtcgttcat cttgataatc aaccggcaag gagaatagtt ctttgatctt ctctccatat
     5461 gacatggaag cttctgccct tggtccgatg gaattgttca tattcttagg taatccaact
     5521 acaaaacggt ctacttcgta ctgcgccact aattccttca accgatcaaa accaaactct
     5581 tcttctgcct cattgattcg aatgatttct actccttgag ctgtccagcc aaagggatcg
     5641 ctcacagcaa cgcctactgt cttagaaccg acatccaagc ccattactct cataaattca
     5701 ctccatgatt tgagaggtag tctttgacaa ttacttccat gatttcatcg cgttcgtgac
     5761 ggcgaatcaa atttcgtgca tcacgataac gaggaatgta ggccgggtct ccagaaagca
     5821 agtagccgac aatctgattg attggattgt agcctttttc ttccaaagcc cgataaacaa
     5881 tcgctaatgt ttcgctcact tcttttttgc gactatcgtc aaaaccaaaa cgtacggttt
     5941 catctgtaaa acccatgatt ctacacctct ttcccgcaag gtaattccag cttaccttct
     6001 acacccttat tttactcgaa gaaagggaaa actacaaaca aatattccaa acttctgaat
     6061 aaattaagaa acacgatttg tctgattttt ccttttttct gtggcttttt caaaagccct
     6121 ctttccataa agtatacgct atatttcttt gaaagtatgt ttttacacaa ttttttcata
     6181 aagttcataa aaaattcgaa ttgctgctac taatttttcg ttttcgttgc ttgccctgtc
     6241 tcataattga tggtgtagcc atcttgaaca ttgtagataa agacattgaa ttcaattttt
     6301 ttgtcttcaa tgctttctgc ttccaactgc acacctcttg ctaccagttc atttccctcg
     6361 aaatgaggag tgacacgata gcgtacatga tgatttgttt ccttgatata tgccgcaata
     6421 tcattttcgt gggcaagcat gtacggagca ttcaacgaac gtgtccctgt cattaagttt
     6481 ttaatgttat tattttgacc agtcaactgg aaaccaatca aatggctgcg gttgtataac
     6541 cattgtccgt cacttaattt cttttgtttc caacctgtcg gatcaatata caaaggttct
     6601 ctcttttcag taggaaacaa gtcttttccc aacatcgcat tggcttgtcc tactctgttc
     6661 aatttgtcta tatttgaata gctttcccat gtccctttgg aaagactaag atcctctttt
     6721 gtaaactccg gctgattccc attgatttct atttcttgat gttttccatc atattctggc
     6781 acatccgcta cttgagaagc ttgcggtgtg gaatcaaaaa tggttgtaat ttgttggaag
     6841 atatctgaat ctttttgtgt ggtggaaata ccgagtgccc ctgcaatcac agcagcgacg
     6901 atccccacga tcgccttggt tgtttttttg ctcatttctt tactccttta ctaacagttc
     6961 cctaaatctt aacatatgtt cccctataaa taaatactgg aaagaaatag aaacagtaaa
     7021 aaaataaaac cagtttttag acggcttaag aatacaaaaa aagagacgga ttcatcgtct
     7081 ctactcaatc acaaaacact atttacaagg tatttattat cattcaaaaa atttttctta
     7141 atactcaaaa ctaaacggct tctgtacgac ctcttggtaa acagtgttca aaagcttgca
     7201 tctgcggtaa taagtccaac caagtcaaaa acataagaag cttttttgct tgactattct
     7261 tattacttga tgcagggcag ctttttcaca gcctctttta agcgggtgac gggaatcgaa
     7321 cccgcgacat cagcttggga agctgggatt ttaccactaa actacacccg cgatacaaaa
     7381 acagtatacg cctctttcac tccttcctca agttttttta ttctttacgc ttccgatatg
     7441 ttttgtagta aaataaaact aatcaatcaa ttattggaga agatgctatg acgggaaaag
     7501 aattagatct gttgctggac acttgtttac tcgctgggaa aatcatgatg gaaagcaatg
     7561 cggagatgta tcgggtagaa gatacgatga gtcgaattgc tttagcttct ggaaatttcc
     7621 gtttggtcag ctatgtcact caaaccggtc tctttattgg tcttgatcgt acctcgacca
     7681 tccgaatgga acaaattacc aaccgttcga ttaatttaga aaaagtagta aaagtaaatg
     7741 atctttcacg taaatatgtt gcttcagagc taacattaga agaactttac tgcgaactgc
     7801 aagcaatcga ccgagaccgt tctttctttc ctgtctggtt gcaaatcgtt agcgcggcag
     7861 tgatcagtgg aacaattatg gtgttatttg gcggaaagat gaccgattta cctgtgactt
     7921 tagtcattgg aggattggga tacgcagctt atctatatag tttgaaattt tttagaatca
     7981 aatttttatc ggaattttta tcttctttat tgattggcat agccgctatc tacagcgtac
     8041 gttttggctt aggaagcaat caggatctaa tcattatcgg atgtgtgatg cctttagttc
     8101 ctggagttca aattacgaac gctatccgtg atttgctagc tggacattat gtctcaggag
     8161 tttctcgcgg aacagaagcg atgatgactg ctacgatgat cggttttgcg attgctttta
     8221 ttttccagct tttttattag gaaaggaaca caagtatggg gacgatattt attcagtttt
     8281 cttttagttt tttagcgact gcggcatttg ccgttattac aaatgtacct aggcgttcat
     8341 tgatttgttg tggtctttct ggtgcttttg gctggatgat ctactggatc tgtgttcaaa
     8401 taggcggatc tgctgctttt ggttcattgc ttggttcttt aggagtagca ttcatcagcg
     8461 atcttttctc taaacgtttg aaaatgcccg taacgatttt caatattccc gggatggttc
     8521 ctctagtccc tggtggttta gcttatcaag cggttcgaaa tctcgttact gaatcctatc
     8581 gagaagcggc tcattatgcg gtccaagcaa ttatgattgc tggagcaatc gctttaggtc
     8641 ttgtattatc agaagtcttt aaccataaca tccgtaattt caaagtaaag cgtgacgcaa
     8701 tcatgatgaa acgaaaaaaa cggcttttta aataaacgaa gtgtaacaaa gaaaaggttg
     8761 tgaaagagac gttcagcctt gagcgatgac aaaatatctc acaaacattg cgacaagtaa
     8821 ccgcaggagc aataagaagt aattatgagt attaaaaaaa gttttgtaaa aatagcttct
     8881 cacattttta cataaaattg acttaatact gagaaactga cttttgaaca ccctctatct
     8941 gtaaaaaagg ggctgcgaca tgatttttgt cacggcccct cttctttatt caaatttttt
     9001 ccaaaacgtt cgttgataat aaacgatcca tagagcggaa agtattagaa ctaaaccgat
     9061 cacaggaata ttcactaacc aaaatggata aaacaaagcc aacattccgt ataagacgat
     9121 aagaataata ctgatgatca atgaacccat catagttgcg actaaaccca aacttcctga
     9181 acctctgtta aataattggt taatattggt ccagttcagc agcagcaacc gataatctct
     9241 agcgaaataa cgtagtgaca ataagtagca gccgaataaa gaaccaaata gcaagctagg
     9301 gataaaaaac aaaggcagct gaaaagacag tccgcttaat aatgcgatcc caccagttaa
     9361 aaaactctgc aatatccatc cgaatctgaa cttttctttt aaatattggt tcatagagat
     9421 tggcaaagaa cggataaaca gaaaattttc ttgatccaaa gaaataagat tgctgataaa
     9481 agaagtctga ttaactgtta agaaagccaa tgcgatccct accaggaaaa caacaccgat
     9541 taacttatta tctaaatcag ttaattttat ttcgcctcct agggcaaaag caagaataaa
     9601 aatgattgga gttaacaagg aagaagaaag aacttgcata atcaaattag gttcttttac
     9661 taattgagca ttgtaactaa ataaaagctg atgcacattt tggttctttt tatgggttct
     9721 ttttattttg aactcactgt ttgaatcagt cagttgctct gtaagctttg gtactatcag
     9781 ctgttttaca gcaaggaaaa atcccagagc cgtgatagct agtattcccc aacccaataa
     9841 accttgttga gaaagtggct gactggcagc atcatagaat ggcaataata tcgaaatcgg
     9901 atgccgatct gttaactcat tttcaatagt ccccgtttga tgattcatcc acatgattcc
     9961 agcaaatgcg atcagcatcg agctgccaag caagatacta gaaactgttt ttctatgttt
    10021 tttaaacact gctgttcgag ccaaaccaaa aacaatcaaa ctagccagac aaaaaagaat
    10081 aatcaaaaat agagaaaaca aaaggattga gataatgata gtaagcaaaa tccctagatc
    10141 agctcgccat ccggttaaaa taaacatgac tagaagcgga aaaacaaaag gaatgatggt
    10201 caaagcaact accaaaatct ttgctgaaaa aacctgttct tgtccaaaag gcagaggcaa
    10261 aaatacttgc aggtcatttc cttcaaaaaa gatattataa atgactgata tactttgagt
    10321 taaacctaac ataccaaaaa gtgccacgta ataagtaaag aaacctggca atttaacaaa
    10381 gttcatagca aacatagcaa atccgtaaat cagtaaaaaa atcagccctg aaaaaagatt
    10441 ttgagacaaa agatagatag ttaatcgctt tccctttttt cctttctctc ttgctttatt
    10501 cgtgagctgt ggattaacat ataaaagatt aactcgaatt aattctaata attgtcgctt
    10561 atacatacgg tccatctcct gtcgcttgcc ttccagccat tttcaaataa atcttttcta
    10621 gcgtttcatc aggtgagcca ttcaataatt cactaactgc cccctgataa atcaattcac
    10681 ctttcttcaa aatagccagt ttatcgcata actgttgtgc ggtatccaat acatgtgtag
    10741 aaaaaatgac tgtttttcct tttgcggcat gagccttcat catttctttc aaatcaaatg
    10801 ctgcttgagg atcaagtcct tgcaaaggtt catccaatac ccaaatatct ggatctggca
    10861 gcaacgtgcc gatcaaaatc gttttttgac gcattccatg tgaaaaactt gcgattgttt
    10921 cattttgatg actatacata tcaaaaagcg ctgtcaattc tgctaaacgc ttttcttttt
    10981 tttgtgtgtc taattcatag gcagctgaaa tcaagtccca atattctcca gccgtcaatt
    11041 gtaaaaaaat atctggggaa tcaggcacat aaccgatttt ttgtttgata ctcaatcgat
    11101 tttccgtcaa tttcattcca tctactaaaa tcgttcccga tgatggttca ataatactaa
    11161 ctaaactttt aatcgtagtt gattttccgg caccattatg tcctaaaaat ccaaaaattt
    11221 ccccttgtct tatcgagaga gacaagtctt ttaatgcttg ttttttcccg taacttttac
    11281 ttacattgat gaattcaatc atttctgtac tccttttgtt tgttattact ttttattgta
    11341 cccttcgtta ttggtggagt aaatgtcaaa aagagtgcat aacaaaaaag gatagaaacg
    11401 actaggctgc tacttgggtt gttctcttct acaggaaaac actcttctaa caggtaagat
    11461 atgagtcttt gttttcttta aaatgattca ttttctatct tttcttgcat aaaattcttt
    11521 agtttatcaa tacttctctt tcccatctct agttgttctc catttgcaaa tgttactatt
    11581 tcgtttactc tgctaataga tgtaatgttt tcagtattaa tgatataagc ttttaaatct
    11641 ttacataaat actcatattc gagtgatttt ttatattttt taattgtgcc gtttacgaga
    11701 atctcttcat ctttcaatac taggcctatt ttatttttat aacctggcat gctcattaga
    11761 taaaggattt catcgtaatg gatatagtga tcgctattac cataacggac agttgtccac
    11821 ctttttttcg tagcatgttg aacaggatag ttttcttttt ctttcaataa gtaactttgg
    11881 atgctttttt tatttttttc ataagtgaat tcttttatca aataaccact aggcgtaatc
    11941 tttcgattaa taacagttat tgctttattc ggatgagacg ttagataaac aattttacac
    12001 ttttcattct tttctctaag catttcgcct agttcgatac cgctataata ggcgttcaaa
    12061 tcgatgtcta ggaaaaaaat atctgaggaa tagatttcat tatttttaat gaattcaaca
    12121 aattgtataa cgttcaatcc ctgaacgatc tcaaatttcc aaggacttaa taaaattctg
    12181 gattcttcca aaatttctac tagcattttt tcaaaaacca aactatcatc caaaataaat
    12241 atacgcgtta ccataaaaag tacctccact cgatatttta ctatcaaaat catttcatac
    12301 aacttttcaa catacatgtt aggaataaat tgtaattctc ttcacaatct tttccaacac
    12361 ctttgaaacc gatatcaaat actttcagga tgctgcttct aaaataaata tcaaacaaat
    12421 gaatacatca tgcaaaaaaa gccagacaac cgacgtcctt gcgtgtcagc tgtctgagct
    12481 atgtttattt attttgttct tttaaaaaga acgatacaca tactgatttt tcggagcaga
    12541 gactttctca gcgtttgtaa caactacaga tgggatttca cgcttgaatg gttgataata
    12601 gtctgattgg atggttcctt ctattttata ccattcatca tttttaaccg ttactccttc
    12661 cggcatatgc gtcaacaaac caaagactcc tgaatctgcc acacaatgga taatgccgaa
    12721 tcggaacaag aagatatctg tattgctatc gttcggtgtt tggtaaacaa atccttcata
    12781 gctgattttt ttgccaatga attcgctagg gtaattataa attagctcca taacttctaa
    12841 atagttttca tctgtgatct tgatagtttg cttatcccca tacttttctt tcaactcatt
    12901 catttggtct aagtagtctg atttgttgaa ataaatactg gtatccggct ttaagtattg
    12961 tgtctgcatc tccggatccc ctactgattc tttacttact gggaaattaa atcctttggc
    13021 ttctacgatc gtagtgtcga gactaactgt tggaaacaac gtccccacga ttactggcaa
    13081 agctagcagt acataagcga ttccacgttg ataaggtttt gacaacccat ggtcatgatc
    13141 gtgctcgtgt gccttttctt ctgcttttcc gtttttgacc cacaaataca gctgaactaa
    13201 agccaaaaga gcagataaaa tcattgaaag aatagctaag tagcgataat ggacattgat
    13261 atattgatcg agtttgccag aaacttgcaa gtacatcatc aattcgaaat acccagataa
    13321 aatcaaaaac cgcagcatat tttccctcct aaataaataa tgtgaatcca ataacgctaa
    13381 gggcaatcag tccgatcagc gataaaataa agcgtcctgt aaaatatcgt ttcatcatca
    13441 gtagattttt gatatcgacc attgggccaa agaccaaaaa ggcagctaca ggtgctgtgc
    13501 cgaacagact taacaaagaa gatcctataa acgcatctgc ttctgagcaa agagacatcg
    13561 taaaggccaa taacagcatg atgataatgg ctaaaatctt tgttgagccg agcgttagca
    13621 taattctagt tggtaaataa gtctgcatcg cagctgcgat taatccgcca atgatcaaat
    13681 aacgacctgt atcaaaaaat tcgtcgattg aatgtgtcaa cacatgccca gtttgatgga
    13741 tcagctgatt ttttggttca ttcttctcac tttgttcttc ttcatggaca tgatgatggt
    13801 gattttctgc tactttagac gtgatttttt ctttcaatac agcgtctttt tgaaaatagg
    13861 caagccagac accaatcaca agggcaacaa aaaagctgcc tatcacccgc cagcttgcca
    13921 tctgccaaga attgccaaat gcgataaaag tggaaaaaat cacgattgga ttgatgatcg
    13981 gagcagtcag cataaaagca aacgctgtgt gaactggtac gcctttttta acaaattgat
    14041 gaacgatcgg tacgatgccg cattcacatg aaggaaagaa aaatcctaag atcgaaccag
    14101 taaaaataga gaagatacga ttttcaggaa gccatctctt cacacgttct ggggtcaaaa
    14161 atacttgtaa ggcgcctgag ataagacagc ctaataatac gaaaggtaat gcttcaataa
    14221 cgatcgataa gaaaatcgta ctcatctgca aaaccgaatt tggaataaaa gaaaacatga
    14281 gtttcctcct ttttatttct agttcattcg ctttgcaaat atacgctttt attcaaaaaa
    14341 aacaagttgc tctgtaaatt tttacacaac tcttattttt taccaaagca cttcaattga
    14401 ctttttctgt tattcccatt aaattaaggt ctcatacata tcacttctat cataaaaggg
    14461 agaataaaaa tgaaacaaac cactaacttg actgaagttc aagacatact gcaacagtca
    14521 tccgttgccg tcatttatct ttcgatgcct aattgcagtg tttgtcacgc tgttcgtcca
    14581 cgactagagg aacttctcac acattatgat attccggcac ttcatttgga tgcatttgaa
    14641 acaccagaag tagctagtaa atttgaagtg ctgactgcgc cagttgtcct gatttttcat
    14701 caaggaaaag aagtctttcg gcaagcacgg ttcatcgact ttaaaaaaat ccgttatcta
    14761 ctagatgaac tgacagcaga agatgattcc ctttcttatg aagaaatctt ccgaacaaaa
    14821 taatcgttgt ccaaacagaa aaaagcgatt gaacaagtga aagttttgcc ttcatttgtt
    14881 caatcgcttt ttatttttat tctgctgtat tttcggaatg cgcatttgcg tcagaatgca
    14941 agaaatagat caaggtttgc aattcattcg tcaaatcaac attttggatc aatacatctg
    15001 acggagcatt caatcgagca gcggtaaagt tcaagattcc tttgattcct gcttccgtta
    15061 actgattgac tacttcttgt gctctttcag cgggtatcgt taaaattgct acttcgattt
    15121 gttggatctt gatttgttct gacatatctt ccattggata aacggggata ccgtctacga
    15181 tgcgcccaac gatttcctca ttcacatcaa aagcacaact gatacgtatg ctattacttt
    15241 gatggaattt gtattttagt aaagcacttc ccaagttccc tacaccgatc agcgcaacat
    15301 ttgtcaattg atcgtcgttt aatgtctttg caaaaaagtg cataagatct tctacatcat
    15361 aaccataccc acgttttcct aattcgccaa aataagaaaa atctcgacgg atcgttgcac
    15421 tatctacttg aacggcttca cttaattcag ttgaagacac ttttttcttt ccagcattat
    15481 ttaaaatcct taaatagcga taatacaaag gcagtctttt agctgtcgcc tttggaataa
    15541 tatgatcttt cacgaaatga cctccacgta ttgtctaatt acatctttga tttctccatc
    15601 atagcagtat ttataggaaa aaatcaattc cattgctcag atattgtgaa attttattct
    15661 ttattcattt atttatcatt tctgcaaatc tcgcagtata atggaattta tggtagacta
    15721 ctaattgaaa aatagttttt tatttgagga gaattcacaa agtagaaaag aggattccat
    15781 gatattatta caagccaatc agattgctcg ccattttggt gcagaagtct tatttgaaaa
    15841 catacattta gaaatcagta caggcgctcg tgtcgcttta gttggcagaa atggcgctgg
    15901 aaaatcgact ttattaaaga tcattgctgg aattgaagca cctgatgaag gaaagatcgc
    15961 taaaaataaa acagcgactc tcggatacct cgctcaggac acaggacttg attccaatga
    16021 aacagtttgg aacgagatgc tgaaagcttt tgaacaagtt agacaaatgg aagagcggat
    16081 gcgcgagcta gagattttca ttagcgaagg tacgccagac acacctgctt atagttctta
    16141 tttaaaagaa tacgaccggc tgcaacacga tttttcagat cgaaacggct atggttatga
    16201 aaatgaaatt cgttccgtat tgcatgggtt taaattcgat tcttcatttt atgatcagaa
    16261 aatcgatact ttatcaggcg gacagcgcac acgtttagct ttagctcgca tgcttttgca
    16321 acaccctgac attttgattc tagacgaacc aacgaaccat ttagatatcg agacactttc
    16381 ctggcttgag aattatctgc aaagctatac tggtgcatta ttgattgttt cccatgaccg
    16441 ttatttttta gataaagtag tcaatgaagt ctatgaatta agcagaagaa aaatgcgcca
    16501 ttacaaaggt aactattcga aatatctaga tttgaaagca gaacagttag caagtgaatg
    16561 gaaggcttat gaaaagcagc aaacagaaat ccataaatta gaagacttcg ttgctcggaa
    16621 ccttgttcgc gcctctacca caaaacgagc gcaaagccgc agaaagacat tagaaaaaat
    16681 ggaccggctg gatcgtcctc aaggggatga aaaacaagca aactttctat tcagtataga
    16741 aaagccttct ggaaatgtag tcttgcaaat cgaagatgcc gctatcggat atgataatgt
    16801 caccttatct gaaccaatca atctagatat ccgtcgtcaa gaagcgattg ctctagtagg
    16861 tccgaatggt atcgggaaat cgacgttgct gaaatcaatc attcaccaga tcccttttat
    16921 ccgtggaaaa gaaacgctag ggacaaatgt caccatcggc tattacgatc aaggtcaagc
    16981 tgatctgcat acaaacaaga cgatcctagc agaattgtgg gatgagcatc ctaccactcc
    17041 agaaaaagag atccgaagtg ctttaggtgc tttcttattt tctggcgaag atgtagaaaa
    17101 accaatccct ctgctaagtg gcggtgaaaa agcacgggtt gctttagcca agttagcgat
    17161 gaacaaagaa aattttttga ttttggatga accaacaaac catttagata ttgacaacaa
    17221 agaagtatta gaaaatgcat tgatcgatta tgacggcaca ctgctttttg tttcccatga
    17281 ccgatatttc atcaatcgga tcgcgacaaa agtcgtcgaa ctctctgaaa atggcagcaa
    17341 agtctatcta ggagactatg attattattt agagaaaaag caggaagaag aagaaatcgc
    17401 cgaattgttg gcacaagatg agccagaatc tgctcctgct cccacgaaca aatctgatta
    17461 ttaccaaagc aaagaacagc aaaagcttat ccgtagcctt caacgaaaaa ttactcaggt
    17521 tgaagaagaa atggcgcgga tcgatgaatg gatcgatcag ttggaacaag aaatggtcgc
    17581 gccagaaaac ctggaagatc acgtaaaact caatgagctg aaccaagatt tagaagctgc
    17641 tagacaagat caagaaacaa aattgacgga atgggaagaa ctaagcttgg aattagaaga
    17701 atatgaaaat taacgaaaaa accaaaacag aaaccattca tgctgttttg gtttttcttt
    17761 ttttatttta tcaaacaaaa attccatatt tacctttttc accaataaga agaccaatta
    17821 ctagtcattc tcactaaagc ttctaaataa tagtaatcac cccaaataca aggtgtattt
    17881 accccattat ttttgggctt attataaaca gactgtgtta aaaagccatc ttcttcttcc
    17941 gttgtagcat atctttcttt caattgtcgc ataattttcc aaacagcaga ctggtataag
    18001 ttcttttctg ttttgtctaa tagacctgat tctattaatt ctaataatcc acaaaccatg
    18061 atcaccatac tagatgtatc tttttcttct ctgctagaat catcaaagat caaatcccaa
    18121 taacaaatgg tatcttctgg caaccgattc ataaaataat tggctaatat tttgcttaaa
    18181 tcaaaatatt tttgttcttt caaataacga taacttaatg caaaaccgta aatcccccat
    18241 gcttggcctc tagcccaaca ggaattatca ctataacctt gatgcgtact tccatatctt
    18301 ggttttccat ctttgacatc catataaaat gtatgataag ttgaaccatc ttctcttact
    18361 aaataatctg ctgcattttg aatatgagcc tgagcagctt ttttatattt ttgatttcca
    18421 gtttcttcac tcgcccaata caataatgga agattcatgc aacaatccat gatcattcta
    18481 ccttgttgtg aaggatcact aggatctccc caagcttgta taactcctgc cttctcccaa
    18541 aaacgctgaa gtaatgtttc tgcagcatca attgaagctt ccttcatttt tcgatttccc
    18601 gttattttat aatctgctac ggttgataaa gaatacaaaa aaccaagatc gtgatgatta
    18661 gtgataattt gacagtcaat cctgtggata aaatctatat ctgctttttc agctgtttgt
    18721 tgaaataaag gatcacccgt cttctcaaat aacaaccaat taattcctgt ccaaaatcct
    18781 tctgtccaac caatattgtc tcttaatgga taacgtcctt ttataggaca ctcgcttggg
    18841 aattgatact gactgttact gatacgtttg attaacctct ccgtgtgtct gcgacaacgc
    18901 tcgatctcat tattccattc tttttccatt aattctttat aacattcatt tatccatttt
    18961 tctggatgta ttatctcttc aaacatcact ttatcaagca tttccccacc agcctttagc
    19021 ataattttag tcttttttta ttgtttttat tttggatagt tagcccggtt gtcccatcaa
    19081 aatcaatttt gggacgattg ttccatactt ctaaactata atcccgatct tgatgagcaa
    19141 ccactcctgt tccgagccaa tgggttcctt ttccaaatgt accctctaat ccaggaataa
    19201 aagtgttttc ccatgtcatc aagtttgtat tgggcaaacc cataatagct gtggattttc
    19261 gtttaatagt atcgtcactt aaacagataa tacctgaaaa gccagcttca ctcaccctat
    19321 agttttcctt gtcttcttct atgattattc cttctttctc aggagaacat tcaatagcaa
    19381 accctgtctc atatagaaca tattctgtat ctaattctac tttgtgtacg cgaatctgcc
    19441 agccttcaaa aggaataagc caagttctga tttttgcctg taaagtaggt tcccatgtag
    19501 aggcaagtac ctctttctta catacaatgt ctcgtacatt atttcgaacg atgaactgtc
    19561 cataggtttt gatgctctca ttcttaaaac tggtcgtgat atatgtgtcc gctcgctgaa
    19621 tccctagcat tgaatccatt gccatttctg ctttcaattg attcgctcgc ggtatcgaaa
    19681 aaccaaattg actagaataa gcaaatttta aatatttttc attggtcaat gtatggaaat
    19741 ttggccctaa ttgtcccgca ttcaaaaaaa atgtatgtcc attatcatgt tgtatgagca
    19801 aattggcttc tgaaagaagt ttagttcctt cacgaatagg cataggcatt tctttagccg
    19861 tccaatactc atggtcttca ggtagcgcca acaataaaaa aatcttattc agccataatg
    19921 gagataacgt actattatag ggttcagtca atgtcaattg agggtaggta taaccaatat
    19981 tcaggatacc tttttcatca aatatttctt tttgcatcca ccatcttata ttccgattga
    20041 ctaacccctt gatttcacct aatttaaaag gcaaaagatc ggcccacaac atagctgacc
    20101 aaaagcttga aactgcaaaa cgataaatca tgcttcgacc aaaaggcaca tttgcaccat
    20161 cttccgcaaa aaaatgaata tagtctctag aaaaatcaat agctcggttc ttatatatct
    20221 ggctaatggg cttttctggt tctagtacgc tataaatcaa accataataa tgaaatgcaa
    20281 aaggattata ataatcttgg cgacctcttg aactgtctcg ataccagcca ttttgaagat
    20341 aacaattttc tatattatca aatgcttctt tcagtgctga ttctttaact ggataaccaa
    20401 atttttggat aattgttccg acaattatct taaaaaattg ccaattacca tctgcacatt
    20461 catattcaaa aatttgcgag aaccaatgaa taatctggct tttttctcct gtatctaatt
    20521 ttttccataa taattgttca tgatgatata acgctaataa cattgcgggc atttctacca
    20581 tacgctggtc tctttcatgt atttctcccc aataatcggg atgggaagga tctgttccag
    20641 cttttattcc tgatagaatt aatttcaacc atcgttcatc taattggtca attgcgggcc
    20701 ccgctcccca taaaacccga gagaatcctt ccattcctgc aattttattt ccatatccta
    20761 ccccttgatg tatataatca atttgtgcgc cacttgctcg ataaaacggc tggagtggtt
    20821 gatttatctt tttaaataag tcaaagtaat cctgtctggt ccttaatagt ggtactggat
    20881 gtttcattgt attttctcct ctttttacta ccaaatagct aattccgact gttctaatcg
    20941 aagtaagcct tcaataaaaa aataatcaga atagatcagt ggcacattat aagtcagttc
    21001 tctatggtat gcttctgctc cgcctccaat gatcccatct tcatccatat tccaatttgc
    21061 atagtgcttt tctgtctttt ggagtatatt gatagcagct tcatgataca tttttgatcg
    21121 tacagaggaa acatatcctg ctaattccag aagaccgcat gcagcgcata ctccagcact
    21181 ggtatcaata ttcgaatctt ttacagcaaa atcaatgaga ggaatataac cagttttttc
    21241 gattttctga ataaaattta ctgctatctt ttctgctata gctagatagt acggattttt
    21301 tgtgtaacga taacttaacg cgaatccata taaagcccat gcatttcctc tgctccaaca
    21361 agaattctca ttgtatcctt gcccgccaag taaccgaata tatttccctg attctgcatc
    21421 aaattctgcg atatgcccgg ttgatccatc tttcctcact agatacttag ctgttgtatc
    21481 tgcatgctgc cgagcaattt tagagaacct cggatcacca gtttccctcg aagcccaata
    21541 cagtaatggt aaattcatca tactatcaat aatcatccaa ccaggtttat ggtcattcca
    21601 tgctactaaa tagttccctg aaatattata gcgtccagca agtaccgtag ctgcatgtaa
    21661 ccctagttgt cgactatgtt tgttttgaga ctgttgataa tgtgcaatag ccgatggtaa
    21721 gaataaaaaa cctacatcat gatgaacgtc tgaaaaatgg tacagggcag attctaataa
    21781 ttgttcttgt tgtattgctg cctctttgaa cttttctcgt tttgtcttgt gaaagagctg
    21841 ccataaaata ccactccaaa aaccattggt ccaccagtcg attcctctgt cctttattac
    21901 gtctgtatat tttccgttgt aagaaatata tggaatttta ttccccattc tgtctaattc
    21961 cgcatctatc ttttcagaag ctacattcaa aatttttctt gaccattcct tcgtctgttc
    22021 attcatggat atcccgccat tcttttaatt tttatgaatg tggagttgct cctagaacga
    22081 actagatggt taacattcgt tatttttttt acttatttgc agctccaatg tcattctcct
    22141 taaatttaga ctgcgcccac acataaatca agaccattgg gatcactgaa gttgctaaaa
    22201 ttagcataat tgcgccataa ttaattggct cacctgtact agaaaccgtc atatttttga
    22261 taattaaggg aaccgtatat ttatctttat ctgaaagcat gactaatgga aatagatatt
    22321 gattccaact tcccataaag ataagtaaac cactagtaat aatcgtaggt gataaaatcg
    22381 gtacagcgat tttcgtaaaa atcacccact cattggcacc atcgattctg gcagcctcca
    22441 tcagtgctgt aggaaattga ctgctatatt gtcgcatcat aaacactgaa gttgtactgg
    22501 caatcgaagg gatgatcaca cctgcataag tattcgataa tcctaaacga ctcataatgt
    22561 aaaataaagg aatcaatgtg gtaaaaaaag gcaccatcct agatagcata actgtattga
    22621 acaacagttt attccccata aaacgatact ttgctaatgc ataacccgcc attgtagaac
    22681 tggctgtagc tgaaactgta ctgattacag caataacaat cgaattccaa ataatcaagg
    22741 gataattata tctcgccatg atggcttgcc agttttttaa tagcatatcc ccaaacttga
    22801 aattgattcc gccacttaaa atttcttgat tactttgcgt tgaagctaca atcatatata
    22861 aatatggcat caaaaatatt aaggcaccaa acacaataat gacgtacgta aaaattctgc
    22921 tttgtatctt ttgtttagtc atttttatca cctccgaatt taaattgaat agccgataat
    22981 ataacagata caaacaccat cgtccaggca atagcagagg cttttccaaa atcaaccatt
    23041 tcaaaacttg ttttatataa attgactcct acgaccaaag ctgaattttc tggtccattg
    23101 gaagtcccaa agaatatatt tgggatattg aaattaccca agccattgat agtggctaaa
    23161 actgtggcca ttaaaatgat tggctttagc atggggatcg ttaataaaaa gaattgccga
    23221 aatttcgatg ctccatctat ttcagcactt tcgtatatct ctttagaaat cccttgaaga
    23281 ccagctaaaa ataaaatagt gttatatcct acactaagcc aaatcatcgt gatgatcatt
    23341 gatattcgta tccaaaaagg atttgttaac cactgtacac cgttaattcc tagcgcattc
    23401 aaaaatccat tgaataaccc agtcggattg aaaaaagtca tgaatacgga agctacagcc
    23461 accgttgatg taatggttgg aatataatag acagttctaa aaaatccttt tccttttaac
    23521 tgtgctgaat tcaataaaac agcaaagtaa atagctaata gcatcgttat tggaattgat
    23581 cctgccatga taatcaaaac attcgtgtaa gatttaataa acaatggatc tcggaagata
    23641 gctagataat tgtcaaatcc aacaaattca aatgagtagc caattttcat aaaactaaaa
    23701 gcaaacgaga taatgatggg aatcaaaatc gtcacaataa agaaaataac aaaaggtgtg
    23761 accaatccat aagcactttt tttatgagac gtatctgttg cttttttctt gctgttcttt
    23821 attgtcttgc ttggtaacga agattgcatc atcgttaagt cctcctaata ttttttaatc
    23881 accagttaat tttacgccgt acttatgagc aaaattttct gcttctttta gatatgaatt
    23941 ttcaaaatca cctgtttgcc aatagttata tgcagcggta gtcaaataag acgtagcatc
    24001 tgaataagtg gctggtaaaa cagtatttga tgtattttca ttgattttat tcaatagttc
    24061 atagtatttt tgtcctcctt agtagtcaaa ctttttctca gctgcttctg tttgataggc
    24121 caatgagtta gatgtagcca cgcccagctc cagtgcgttg gctaagcatt cttcatctgt
    24181 cagcataaat gtaagaaatt gttgtgccac ttgtggattt ttggcatctt tagggacata
    24241 aaaacttgat cctcctgata ctgcaccttt tcctggattt tcttttgaaa aaggaatcag
    24301 tggagccatg cgccatttgt cagatgcatc aggaaaattc aaagacatat tcgtagcata
    24361 ccagccgcca gcaacaaaca ttgcagattc ttgaaatgct ttctcagcat cttgtgcacc
    24421 gtagaaatgt acaatatctg agtcgatcat ttctttgatt aactttgcag catgtttcgc
    24481 ttctttagta ttcagacgga tatttccttc ttcatcaagc aaagattgat tctgctgggc
    24541 tagcatgttg ataaacaatt cttggtcccc agattctggc aaggcaattc caaaactacc
    24601 agttttttct tttaatgctt ttgccacctg gatatattct tcccaagaac tgatttcttc
    24661 aaaatctaca cctatttcag caaatttttt tggttgataa taacaaagtg ctggggtaaa
    24721 atcactagcc caaggaataa tcgtttcatt actcgtttgt tgacgcaaaa tattcaattt
    24781 ttgatcataa aactgatcgc catatttgtc ataaaatcca tattcatcag cactataaaa
    24841 agcatcttga aatttttcca gcacacctgc tgctgaagtg tctgccatgg agataatatc
    24901 cggcatatct tcgtgtgtta ccaattttgg ggtgatcatt tgtactgttg ctgttcccga
    24961 agctacattc acaattttta agtcaatatt tgctccagtt ttgcggttat agatttttgc
    25021 agcattttca acatagtttg tcagtttgcc gtttgcgaca tatacttcta atgtagtggc
    25081 tttttttatc attggatctg cgatgtttgc tgttacttct tcgttagttt ttccacatgc
    25141 acttaagctg aataataggg taatgtttag caaagctatc accaatttgt atcttttttt
    25201 catgtctatc ccttctttct tttcaagttc ttatataaaa cgctttcact tatgtatatt
    25261 aacggaatta gtaacacaaa tatttatata aaaaaatatt ttacttgtcg ttttggcttt
    25321 tcatttttgt tataatagtc attaattata aatagaaagc tttttatata aaaagagaaa
    25381 ggaaagtggt tcttttgtta acaaacaacc aatcctccaa tcttgtcaaa aaaacactaa
    25441 cctattccat agatacccat cttcatttat ctgctgctcc ctattttcga ctactaataa
    25501 atgaaagcgc taatttatat gctgaatttg cacataaatc tatagaacta gataatcctt
    25561 cccttttatt cattgataaa cagcaagaag tccaaatctt ttctactcaa aaggaaacac
    25621 aaacttttat cattatccag ttttattttg atgagcaatt attagcacat attacgggac
    25681 aatctttttc tatagatcaa gaaataacat ctatcattac ttttctgaaa aatgaacagt
    25741 tcaaaattga ttatctaaaa catagtaagc cagctatttc ctatgatcag gaaatcaatt
    25801 cttttttcat actatgccaa cagatgagca ttcatttgct ttccttatta gttacttatt
    25861 gtctgcaaaa aattcaattg tcttccttac caaggcatta tcaacaggaa cttgctttat
    25921 tgacaatacc ttcaaatgag aaagtcattc ctaaaaaaaa gcatattctt tattcagatg
    25981 aattagttga tccaatcata cgatatttag aagaacatct ttcagaaaat tttttgcttg
    26041 aacaattagc ccaaattttt tttatcagtc cagccagctt aaaaaaacat tttagaaaag
    26101 tgaatggaaa aagtgttatt agctatttta aagaactaaa aatggaaaaa gcaaaacgtc
    26161 tcgtgctaga aaatgaatta acctatacag aaattgcaga tatattagga tttcattcga
    26221 tccatcattt ttcttcagct tttaaaaaat atacagggtt atccccttca aagtattatc
    26281 tatccttaaa atagaaaaaa atttttaggt tttatccaca aattatttct ctatattttc
    26341 tgtctattca aaataaacaa taattttccg gcacaattat ttgctgtttt ttaccataac
    26401 atagtacgat caacgtagaa aaggagatgt ttttatgaaa gttgttgtca ttggcggtgt
    26461 agctggcggt ccttcatttg ccactcgttt ccgtcgattg aatgaagcgc acgaaatcat
    26521 tatctatgaa cgcggagaaa atatttctta tgcaagttgt gctttgcctt attatttagg
    26581 aggtgtgatc actgaccgtg actcgctgat cgaacgtaca ccagaaatat tgaaaacaaa
    26641 aaacaacatc gatgtcttta ctaaacacga agtaacagca atcgatcctt ctactaagcg
    26701 attaacagtt aaagacctat ccacaaatga agaaataaaa acagattacg ataagttgat
    26761 catctcttct ggtgctagac cagattatcc ggatattccc ggagtttttg aagcagaaaa
    26821 cggctttgtc ctccgtagtg tgacggatgc ggatcgaatc aaatcgttcc ttgaagaaaa
    26881 aaatccacaa catgtcgtca ttcttggtgc aggtgttatg ggtctggaat tagctgagaa
    26941 tttcaagcat cgcggcttag acgtgacttt aatcgatcaa ttgccacaag tcgctttccc
    27001 ttatgatcca gaaattgcta atttagttta tgacaaattg ctgaaagaag gattagccgt
    27061 tcatttagaa acaagagtta ctgagatccg tgataaaggt cgagaaatag tattatcaga
    27121 tggctccgtc ctttctgctg atatgctaat ttttgctgtt ggtgtttctc cgaagaatga
    27181 agtggtgaaa gcaaccggca tacaattatc agatacaggt cagatcattg ttgatgacca
    27241 gttacaaacc aatctgccgg acatctatgc gattggcgat attatcgaaa cgactagtgt
    27301 agtgactggt cagccgatcc aaagtatgct ttccagtgcg gccaatcgtc aaggacatat
    27361 gctggcagat attttaaatg gtacgcctat gcgctatcgc ggatatattg gtgcaggtgt
    27421 cgcaaaaatc tttgatcata cagcaagtta tgcgggaatg acagaacatg aattaaaagc
    27481 atcaggcata acaaattata aaactgtttt tatcactccc tttgaccatg cctatttcta
    27541 tccagaagct acaagattaa atctaaagct gatttttgat gcagatagcg gtcgtatttt
    27601 aggtggacaa gcatttggag aaaaaggtgt cgataaacgg atgggagaac tttctgtagc
    27661 aatcaccgga aacttgacag tctttgattt gcctgatttg gagttgcctt actctccacc
    27721 gtattctact acccgtgacc cgttgaatat agctggttat gtcgcaatca atcaaatgac
    27781 gaatatcgta gaaacgatca aagcaagtga tatacccgaa aacgatttga aagaagcgtt
    27841 ctttttagat atacgtgaac ctaataaagc accttctggc agtatctcgg caacaaaaaa
    27901 tattccaatg aatgagttac gagatcgaat caatgaaatc ccaaaagata aaaaagttta
    27961 cattactttc agaagaggat tgaatactta tacttctgcc cgaatcttgg caggtttggg
    28021 tatcaaagcg attttgattg aagaataaaa aaacgaccgc ggtgggtagc cgcggtctag
    28081 gagttatttt tacttgagga gtaaaaatga aaaagtatat agggttgttg gttggtatgt
    28141 atatagaata gaagataaat gtgaagaaat tgtgcattat ttcttcacat ttattaggtt
    28201 atagtttaag aaagtttaaa cattgcgcaa tttaattatt ttagctgttt cttttcataa
    28261 aaaaacatgt tgtgactctg agacaaatca tagtccatag tgatgtctct agagtaacta
    28321 cgcctaatat tacgaggcat catattaatc tatcagcact agtacctaaa ctagcatccc
    28381 tttcgaatat tttttcttat ataataaagt ttgctttcac tcctctaatc tcaccaaatc
    28441 gactggcttg cgttgtgtga tactaggaac tgattggttt agaacgactt ttctattgta
    28501 atcatatttt tcgccatcct cgttgtcttc taatccttca atgaatgatc cctaatgtgt
    28561 gtattaaata atttaatttt tttgttccta tttatttaa
//
LOCUS       DAZKNH010000029        26980 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000029 DAZKNH010000000
VERSION     DAZKNH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.029"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(148..2301)
                     /locus_tag="ACG8MG_001174"
     CDS             complement(148..2301)
                     /locus_tag="ACG8MG_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFQ0375231.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2595..3443)
                     /locus_tag="ACG8MG_001175"
     CDS             complement(2595..3443)
                     /locus_tag="ACG8MG_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0375232.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="ACG8MG_001176"
     CDS             complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="ACG8MG_001176"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFQ0375233.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5944..6123)
                     /locus_tag="ACG8MG_001177"
     CDS             complement(5944..6123)
                     /locus_tag="ACG8MG_001177"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFQ0375234.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6170..7993)
                     /locus_tag="ACG8MG_001178"
     CDS             complement(6170..7993)
                     /locus_tag="ACG8MG_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0375235.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8271..10550
                     /gene="helD"
                     /locus_tag="ACG8MG_001179"
     CDS             8271..10550
                     /gene="helD"
                     /locus_tag="ACG8MG_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFQ0375236.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10643..11290)
                     /locus_tag="ACG8MG_001180"
     CDS             complement(10643..11290)
                     /locus_tag="ACG8MG_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375237.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11469..12188)
                     /locus_tag="ACG8MG_001181"
     CDS             complement(11469..12188)
                     /locus_tag="ACG8MG_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0375238.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12319..13470)
                     /locus_tag="ACG8MG_001182"
     CDS             complement(12319..13470)
                     /locus_tag="ACG8MG_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFQ0375239.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13687..14427)
                     /locus_tag="ACG8MG_001183"
     CDS             complement(13687..14427)
                     /locus_tag="ACG8MG_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0375240.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="ACG8MG_001184"
     CDS             complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="ACG8MG_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFQ0375241.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14811..15404)
                     /gene="yqeK"
                     /locus_tag="ACG8MG_001185"
     CDS             complement(14811..15404)
                     /gene="yqeK"
                     /locus_tag="ACG8MG_001185"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFQ0375242.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15388..16038)
                     /locus_tag="ACG8MG_001186"
     CDS             complement(15388..16038)
                     /locus_tag="ACG8MG_001186"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFQ0375243.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="ACG8MG_001187"
     CDS             complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="ACG8MG_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFQ0375244.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="ACG8MG_001188"
     CDS             complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="ACG8MG_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFQ0375245.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17481..18008)
                     /locus_tag="ACG8MG_001189"
     CDS             complement(17481..18008)
                     /locus_tag="ACG8MG_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFQ0375246.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18188..18973)
                     /locus_tag="ACG8MG_001190"
     CDS             complement(18188..18973)
                     /locus_tag="ACG8MG_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFQ0375247.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18966..19835)
                     /gene="accD"
                     /locus_tag="ACG8MG_001191"
     CDS             complement(18966..19835)
                     /gene="accD"
                     /locus_tag="ACG8MG_001191"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFQ0375248.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19836..21215)
                     /locus_tag="ACG8MG_001192"
     CDS             complement(19836..21215)
                     /locus_tag="ACG8MG_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFQ0375249.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="ACG8MG_001193"
     CDS             complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="ACG8MG_001193"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0375250.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21667..22143)
                     /gene="accB"
                     /locus_tag="ACG8MG_001194"
     CDS             complement(21667..22143)
                     /gene="accB"
                     /locus_tag="ACG8MG_001194"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFQ0375251.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="ACG8MG_001195"
     CDS             complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="ACG8MG_001195"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFQ0375252.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="ACG8MG_001196"
     CDS             complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="ACG8MG_001196"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFQ0375253.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="ACG8MG_001197"
     CDS             complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="ACG8MG_001197"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0375254.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25173..25397)
                     /locus_tag="ACG8MG_001198"
     CDS             complement(25173..25397)
                     /locus_tag="ACG8MG_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0375255.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25450..26412)
                     /locus_tag="ACG8MG_001199"
     CDS             complement(25450..26412)
                     /locus_tag="ACG8MG_001199"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFQ0375256.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26422..26862)
                     /locus_tag="ACG8MG_001200"
     CDS             complement(26422..26862)
                     /locus_tag="ACG8MG_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0375257.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         7363 a         5988 c         4690 g         8939 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaac caagaagtat ttaatatttt
     2281 tttcataaaa tctttcatca tttcctatcc ttccgcctat ttacattcat gcttcattgt
     2341 acccaaaaac ttcttaataa aaaagaaaaa atgggcggag caagaaattc tttacctaat
     2401 ttcaagataa aatcaacttt ttgtttcaca gcaatcactt aaagttacat atccaactat
     2461 ttttttcatt aaaaaataaa cttatttttg aaaattatgt tttcttaagt cgtgaaacac
     2521 tttgatggat catttatttg tggaaaacta cgttttgtta ttctaaaaaa cttatataaa
     2581 aaatagtaag gtttttattt cgaatccata aaatattctt ggattgcttc tcgtgttttt
     2641 ttcacttttt cgctaagctt ttcgttttta gccatataga aataaaaaat gatatcgatc
     2701 gtagcaaatt gggccaaaag tgaattagtt gccgcacttc gattcgtgat ttcttttggt
     2761 ctagatgttt gtacgagtac atcggctatc ttgctaagcg ggttatttcc aaattgcgtc
     2821 aacgcaatga tttcgatatt catgcttttc gccagctcgg ctaatgcgac tacatctgca
     2881 cttctacctg agtttgaaac aagccaaagc actttctttt tctcattgct gacaagttgc
     2941 gggagcaaga cgtggatatc tttttcaaaa atgattggct taccgacacg agaccatttt
     3001 tgcagaatat cttctactac taaagaagag gcgccaaccc catataaaaa aacagtctct
     3061 gccgattcca aacactctac gactttttgg atcgtttctt cttctagaat atctactgtt
     3121 tcttttatcg ctacttgtgc attgttacca agtttgtttt tgatagagta aaaggactca
     3181 tttgcttcta tatcactaaa accttcatcg attccagtct gtagttctgt tgaaatcgtg
     3241 attttgaagt ccgtcagact ttgaaaaccg atttttttgg aaaaacgtac aactgtcggc
     3301 gcactcaacc cggaagcttc tgctaattca ttagctgtca ttgtagggac cttctcaata
     3361 ttaccgataa tgtagttggc caactttttt tctgctgaag aataatcttc catcatactt
     3421 tcaatacgac taattacgct cataagttcc tccacccttt taatgatagt ctatgtaagt
     3481 ataccatatc agacccttat tcacggtcgt atactggata aaaaacaaaa aaacaatccg
     3541 cccaacagaa tatcttatcc catcaatccc acagattcta ttgagcatct gtgtctttga
     3601 ggaatacctg ttatctattg ttcggattgc tttctatcta ctactttttt acaggaaatt
     3661 tacctattag ttttctctgc tgtaattttt gacttctgct tccatagcat caataaagat
     3721 attcaaatct tctacagctg tttctttact tccataacga cgtacgttca ctgttgcatc
     3781 ttccaattcc ttgtctccaa caactaattg gtaagggatc ttttgtgttt gagaggcacg
     3841 tatcttatag cccatttttt cattacgatc atcaacttct acacgcaacc ccttcatttg
     3901 caaacgttct ttgatttcat aagcataatc gccatgtgca tcaacagaaa cagggatgat
     3961 tgttgcttgt ataggtgcca accatgttgg aaaagcgcct ttgtatactt ctgttaaata
     4021 agcgacaaaa cgttccattg tagaaacaat tccgcggtgg ataactactg gacgatgcgt
     4081 attttccccg tcttctccaa catatgtcaa atcaaagcgc tctggcaata agaagtctaa
     4141 ttgtatagtt gataatgttt cttctgtacc aagagctgtt ttgacttgaa catctagttt
     4201 tggtccgtaa aaggctgcct cgccttctgc ttcaaagtaa tctagttcta attcatccat
     4261 agcggctttc agcattgctt gggctttttc ccacattgcg tcatcatcaa aatatttatc
     4321 tgtattgttt ggatcgcgat agctcaagcg gaaacgataa tctgtaatat tgaagtcagc
     4381 atatactgcc accatcaatt caagcgtacg tttaaattca tctttgattt gatcaggacg
     4441 aacaaatgta tgaccatcat tcaacgtcat ttctcgtaca cgttgtaaac ctgacaaagc
     4501 acctgatttt tcatagcgat gcatcattcc taattcagcg atacgaattg gtaattcacg
     4561 ataagaatgg atcgtgtttt tgtagaccat catgtgatga gggcagttca ttgggcgaag
     4621 aacaagcatt tcgccatctc ccatatccat tggcgggaac atgtcttctt gataatgatc
     4681 ccagtgtcca gatgttttgt acaactcaac atctcccatt attggtgtat atacatgttg
     4741 gtaacctaga ctaacttctt tgtctacgat ataacgttcg atcgtgcgtc ggattgttgc
     4801 cccttttgga agccagaatg gcaaaccaga acctacttct ggggaaacca tgaacaagtc
     4861 aagttctttt cctagtttac gatggtctct ttcttttgct tcttcacgga gacggatata
     4921 ttctttcaaa gctttcttgt caaaaaaggc tgtcccgtat acacgttgca tcatttgatt
     4981 gtttgagttt ccacgccaat aagcacctgc cactgataat aatttaaata cttgtatacg
     5041 gccagtggat ggaacatgtg gtccacgaca caaatcgatg aattcgcctt gttgataagc
     5101 agtaatcact tcttcttctg gcaattcact aatcagttct actttgtacg gatcacttgc
     5161 aaaaatttct aatgcttcct gttttgaaag aacacggcgt tcgatcggca gattttcttt
     5221 gacgattttc atcatttctg cttcgattgc cggcaaatct tctgcagtta cttggttttg
     5281 tccattatct gtatcgtagt aaaaccctga atcgatagct ggtccaacgc caaagtggat
     5341 atttggatag aggcgacgca tggcttgggc catcaaatga gctgctgaat gacggactaa
     5401 gccaagtgca tcttcatggt ctggtgtgat gatctcaata cttgcatctt cctcgattgg
     5461 acgagtcaaa tcaacgagtt ttccgttcac tttaccagct aaagcttttt ttgctaagct
     5521 ttttgaaatg ctttcagcaa ttgccaatgt tgttgttcca gactcaaatt ctttgactgc
     5581 accatctgga aaagtaattt ttatcataat agtttcctcc ttgaaatgtt ttgattgatc
     5641 agatcatcaa gctgaaaaac aaaaaaatcc tagcatctct tgaaaagaga tactaggacg
     5701 aaaagttcgt ggttccacct aatgttcaaa cgcaaatgcg ttcctcaagc gttgtaacgg
     5761 tccgaccgcc cttgcttcaa caagggtttc gaaagtggtc atcctttggt tttctcctgg
     5821 aaaagtttca gcctagcttt tcctctctta tcgaagactc tcaaattcag ttggctttct
     5881 catcaatcaa ttatgtgttt atttaaccac taataaatag aaaaatcaag tgggaaacta
     5941 aacttatttt ttccattccc catttacacc gtatgttcct tttttcatct cattgatgat
     6001 gacatggatg tgttcttttg gtgcctgcgt gtttttatga acagcttccg tgatatcttt
     6061 catcatattt tctaattgtt ccggactgcg accttctact aattcgacat gtacgaatgg
     6121 cataatgctc tctcctcttt ctgatcgttc ttttttcaac tcgattctat catactatgt
     6181 ttgtcttggg tactgaatca ggaaaaatgg ctggatcttt ttttgctctc aaggcactca
     6241 aaatagagac cgtatccacg acttcttgca aaacagctcc gaataatgcg gggattagtc
     6301 ccgtacttgc aattaacatc aaaaaaatac aaataaagat gccaatcagt acagactgtt
     6361 tcgctattct catcgtatct cttgaaatca ctactgcttt tgctacacgc aagagatcat
     6421 ctttcaagat gaccgcatct gcactttcac tagcagctgt agaaccatga gcccccattg
     6481 caataccgat atctgcagca gcaagtgctg gtgcgtcatt cacgccgtct cctaccatcg
     6541 caacaggtct ttgatcttta gggatacttt ctaaaatggt cagtttatct tgaggcaagc
     6601 tacgggcatg tacagaatct aattggactt tttctgcaat cgtttgagca atggcagatc
     6661 catccccggt gatcatcatc aaatttttca catcaagttt tcttagctcc ttgattgtct
     6721 ttgctgcttc tgggcgaatc tgatccgaaa ataggatcgt tcctgcaaaa gatcgatcaa
     6781 ttgttacata gattgctgta ttggacgttt ctatttcttc agctgaaaca aaagaggcac
     6841 gtccaatctt tacttcatga ttattgacta ctgcttggat tccttgaccg gtcacttctt
     6901 tcagttgact aacaggatac agttggctgt ttttcacgtt gttcactaac gaacgagcta
     6961 agatatggct agattcctgt tctgcactag ctgcatacag aagtagctct tcttttttta
     7021 ttccattgac tggctgaatc gcctctactt ccaatatccc tttggtaagc gttcctgttt
     7081 tatcaaaagc aatcgttttt gtacgtgcga gcttttcaat cgttgttcca gtttttacaa
     7141 cgatcccgtt tttacttgaa cggctcattc ctgcaactaa agcaatgggc gcagctagaa
     7201 tcagtggaca aggtgatgcc actaccagta cctcagcaaa tcgtaaaggg tcttttgaga
     7261 accaccaaga aataccaccg atcaaataag caatcaacgt gaaagggact gcataacgat
     7321 cagctaaacg gacaaatcgg gcaggctttt cttttgattc ttcgactagt ttaataatca
     7381 tttgatactg gctgtctgcg gcacgttttt cagcgatcat ttggatcgat ccgtctccat
     7441 ttaaagaccc tgacataatc tgatctccag gtttcttggt ttccgggcgg gactcacctg
     7501 tcaaagaaga ttcatcgacc gaagattctc ctttggtcac tcgaccatct gcgggtacaa
     7561 gttcgccagg cttgactagt aactggtcat caatatttat ttcatcgatt actatatcct
     7621 gcaattttcc ttctttgaat cgatgggcaa ttcttggggt gttatctaac aaagactgca
     7681 gttctttacc cgctcgttta ttcgcgtaat cctctagact atctccgccc gtaagcatga
     7741 tcaatatcat caaactagcc cagtattctt tgacggctaa tgttgcaagg attgcggtaa
     7801 tcgctaatac atctacgcca tatcttcctg aacgtaacgt cttgatcatt tccaaaaaca
     7861 tcaagaaggc gagaatccct ccagtaaatg cgacaatcgc aaatgcaagg gccggctggt
     7921 gaaaaacaaa ttcacttaat aaagctatcc caccaataag caaagtaagt aaaaattttt
     7981 tgaaatgtgt cattgttttt tctcctcttt tatgtgactt agagaggtta gtaataaatt
     8041 tccagctgcc tctttcatct taagcatagt ccttgactag cactttttaa aacaataggc
     8101 cttaattgaa aacaactatc actatcagcg tttccaaaat tcatcagttc tacaaacaaa
     8161 aatattcagc aaggattact ttttcgattc atcagttaaa aagtaccttt tttcgaaaaa
     8221 ctatgctata cttttaattc gttatttacg gcggaaggag caaacatgcg atgaatgaga
     8281 aacaactaga acaaacgcac gtggatgaaa cgatcgaact aatccagatc gaacaaaata
     8341 tgctggatac aaaacaacaa gaattgactc aggaaatgaa tcattcgaca aaagacacag
     8401 ctaatcacaa gattcgagct ggttccaatg aagcttttta tgaatctgct gttgaatatc
     8461 gccaacatga gcaggaattg cttttgaaat accatactct tgaagcacag caaaaacgcc
     8521 tgcaaacgtt gtctgtcatg aaagatagtc cttattttgc ccgtatcgac tttaaagaag
     8581 accgtgaaac agaaacatta tatctaggta tcgcttcttt aagagatctg gcagaagaaa
     8641 caatcgttat tgactggcgc gcaccaattg ccaatcttta ttatgaaggt gagattggtc
     8701 ccgcatttta tgaaactgaa acagatagaa tagatgtcga tctgcagttg aaacgacaat
     8761 ttaaaatcca agaaggacgt attttatcta tggtagatac gtcggaagta atcaatgatg
     8821 actttttgct agagatattg gatgaatcat caagcaacca tatgaaaaac atcgtctcta
     8881 cgatacaaaa agcacaaaat gcaatcattc gggacacaac aagtaaagta atgctgattg
     8941 aaggaatcgc cggaagtgga aagacatccg ctttgcttca acgtattgct tttctgttgt
     9001 atcataatcg caagtggctc gaggctgaaa acgttcttct attttcaccc aaccatttgt
     9061 tttctgatta catctcgact gtattgcctt cattaggtga aagcggcgtg cctacacaga
     9121 cattcaaaaa ttatttggtg cagctgctgc cagagttttc tttactagat gaacagcaac
     9181 aagaaagtgg ttttttgcta ggggaaaaag atccgataca agtgatgaaa agcggattga
     9241 ccttagtcga tcagattgat cgctatatcc aaggcatcac aaactacggt cccttgttcc
     9301 gtgatatgaa aataaatggc cgtacgatcc tatcaaagga atcgattcgc caatggtata
     9361 aagaaacaaa tgaacaatta cctttacatc atagactttc tctattacaa acgaagctcc
     9421 tgaaaaaatt aggcggtctg caaaaagacg aaacacgtca gcaatgggtc aaagatctgg
     9481 cggaagaaca gctccaagaa ctatacgcaa cagatccaaa tcttgaatat acagaacaaa
     9541 aggagcgttc attacgtaaa aaactggcca accaaatcgt acgtaaaaga ttccgtcgaa
     9601 tccatcgagg gatcatgaat tatcaatttg tcaatataac gaaacaatat ctccactttc
     9661 tacaaactgt gccactttct cttctaacac gacataaaat cagcgagacc aggtggcagg
     9721 aacatttgac aactgttcgt acacacttgc gtcaaagaga attgcgtcaa gaggatgctg
     9781 tactattctt tttactgatg cgccgaacat atccagtgac ggttacacac aaagctcgct
     9841 atatctttat tgatgaaatg caggatttcg caccatcaca agtggcattg ctgcaagcct
     9901 tgttcccaaa agcaaatttg actttctgtg gagatctaaa tcaaaatgtc tttggaaatg
     9961 aaacgatcgt tggatcattg gaagaccttt tcgtggaccg tgaagtcact cgtttccaat
    10021 taacgacaag ctatcgctcc acaaaagaaa tcactgattt tgctaatcat tttattgtga
    10081 aagaacatca agtagaaaca acagcaagaa aaggagacct tcctttgctc atccaaggaa
    10141 caacagatga tatcaaattg aattggttag cagataccat tcgaacgact cagggaaaag
    10201 cccggtattg gcgtacagca atcatagcta aaacacaaga agaatgcgaa gctctttatg
    10261 ctcaactgcc agacgagttg aaacaacaag tccagctgat tttaaatgag agtgacttta
    10321 tgaagcgttc cattatttta cttccagcct ttttagcaaa aggtctggag tttgaccgag
    10381 ttttcttgtg ggatgtagat gcagaacatt tttcttctca gcaagacaaa ttgatccttt
    10441 acaccatgtg tacacgtgcg atgcatgaat tgaccctttt tacctccaat aatgaaagtc
    10501 ctttgttaaa caatatggat atttcatctt atgagaaaaa agtactgtaa gtaaaaagac
    10561 aactgctatc attaatgtta gcagttgtct ttttatttac aataataatt gcagaaaaag
    10621 taattcaatc atttaaggtc atttattcta tatataaatc caaatctgtt tcaggatatt
    10681 ttaatttgac aagcgaaata atcattttca gttcttccat gttttctgga tattctgggt
    10741 caaatacctt gctatgacgg ccaatcccgc cattgtcgat caatcggtaa attcgattcc
    10801 attcaatgaa ttggtcaatt gattggaaat tgctgattgg atagccgcca caatccaaaa
    10861 tctctgatac acgaaatgct aggtatgcac tatcgatcac tttatttttt ttcgtgtcta
    10921 tcttctcatt tctttcgata agaatttttt caggacgatg tttcaagctc cactctaata
    10981 gtagaaagaa agcctccatc cgatactctt cttcaaataa ccgaaaggct gaatcagaac
    11041 gacgaaaaag cagcggaatc agataatttt gtctataaat cagcattggt gcgcggtcca
    11101 ccatacactc taaataacgg ttcagatacc ctgtaccgac atagtttcct aatcgatagc
    11161 cttcaacgcc gaatcttgcg tatttttgcg ctaattttga acaatcagtt acgttcacag
    11221 ctaactcttc tactttgcgg taaccgctta agatacgttc aaaaggttct aaagtgattt
    11281 ttgcttccat attcttcccc gtccatcttc cacatacttc atatcatttc acatgatact
    11341 tatgctctca tcataccaca ggttgaatta gaaaagaaaa taaatcacac aaaaaagcaa
    11401 gacaatctgt tagtgagatt gtcttgcttt tcgaaaaaga ctgaatactt ttcgttaaaa
    11461 ctggttgctt agtcttctaa tgccacatta tggaacacat gctgtacatc ttctagatct
    11521 tccagtgcat caatcatttt ttcaaatgtt tcttgatctt ctggagatag tgttacctcg
    11581 ttttgaggga tcatttggat ctcagcgatt gaaaaatcag tgattccttt ttcttttaat
    11641 gcttcttgta tactatggaa atccgctact tcaccgtaaa cgatgatttg tccttcttct
    11701 tccattacat cgcgaacatc gatgtctttt tccattaggt attcaaggat ctcgtcagcg
    11761 tcttctcctg caaaagcaaa gattgctgta ttgtcaaaca tgtaagaaac cgctccggaa
    11821 acacccatgt tcccgccatt tttcccaaat gctgcccgta cgtctgctgc tgttcgattc
    11881 acattgttcg tcaatgtatc aacaatcacc attgagccgc ttggtccaaa tccttcatag
    11941 cgcaattcag agtatgtttc atctcctgca ccttttgctt tttcgattgc tcggtcaata
    12001 atatgttttg gtacattata agttttagcg cgatcaatga cgaaacgtag tttttggttt
    12061 gcatgcggat caggatcacc tgattttgct gccgcgtaga tttcaatccc aaattttgca
    12121 taaatccggc tattattagc atctttagca gttttctttg ctacgatatt tgcccattta
    12181 cgtcccatat tttcacttcg ctttctgatt tgatagtttc ataacgcaaa aacgtcctgt
    12241 tccattatac ttgtgaatca cgattctgtg aagctttttt tctttcttcc ttcattcttc
    12301 tcatttttag tcaattatct atactcggat tgggatttgc ccgaaattct gttccggaat
    12361 attgcctccc aattgataga tttgatcact tctaactaac agttccgctt cttgtgcctc
    12421 tttttttccg attcgggaat aaatagaaat ctcttttttt tcttttaaga atcttcttcc
    12481 agcatcggta aatccaagta gtcgaagata atttttattt tgttggacag gaatctcttg
    12541 ctttttgata ttcagcaaga cataacaaga taatcgttgg atccgtgtcc atgtatatct
    12601 ttttgttttt acctttgaca caaaatcctc aaaactatca gcagttgcag ctgctgtttt
    12661 catgcgcgat tctattcctt cggtcatctg atagatatct gtcagttcca gcaaagtaga
    12721 agataacaac cgataacgca aatacggcca ataagattcc caagtctgca gcgaacaagt
    12781 tgaaagatct tcttctgtct gaggcggcac aagtccagct attctttttc cttcttctaa
    12841 cgcatggcga atggctgttg cactggctat cctctgatct gtgatgattt tttcatgata
    12901 gcctgcactt ttacgtcgga tcggatgtaa ttccataggg tggacatact tggcattttc
    12961 ctttgcataa cttaatccca agatatgatt tggcgaagaa aaatcaagcg taatatcggg
    13021 atacagactg cgaaaaacct cagtcatttt ttgcggataa ctatacgttt gatcagacag
    13081 ttcttgaaat gcctcttcta tcctatcttg attctcttgg acaaagcgtc cgaaactgct
    13141 gtaatcaaac gaagtctctt cttctgttcc aaaacacaaa ctgtcacagt ccagtgtttg
    13201 caagattttt actgcacctt tagcaaaata gtcagctgac tgaaccgccc attcgacggg
    13261 aagctccact actaaatcca caccattttg taaagcttca ttcgcgcgat gccatttatc
    13321 gatgatagcc ggttcgcctc tttgcaaaaa attcccgctc attacagcaa tgacgacctc
    13381 tgctcctgtt atttttcgtg ccatttccac atgatagcga tgtccattgt gaaaaggatt
    13441 gtactctacg ataatcccgc agcttttcat gcgcatcctc cttcatagtt atcgataaaa
    13501 aagaaataga gacaagtaaa aaaaggcaaa aatcggaaaa aatacccgaa ctatataaac
    13561 tattctcgca aacacaatac ttctgcgacg aaaagctgca ctgcaagagc aatgcctata
    13621 tcgttcggat atataattta tgttgaagat ttattctttt cttgttaact cgagcaggca
    13681 aaaccgctag tctttgtatg ctacaaagaa ccagcgcttt gatttctctg atgctgcttc
    13741 gtctgtaaaa tcagaaaatg cttcgacttt cgaaaagccg ctgttttcca gcattcttaa
    13801 ataactttcc aatggatagg tacgctcttg atgtagttca tcttcacgaa taaatcgacc
    13861 agacccttct tgttcttcaa cgaaaaaggt aagaaaatgt tcgatgctgt gtttctcgct
    13921 tcctggataa ctatcccaca aaaaagcaaa attatcccct tgataatgat agctatattc
    13981 tggaaaaacc tcatctactt gataggtgga gtgtacgtca aataaaaatc ggccattttc
    14041 ttctaacgct tgatagactt catcaaacac ttgctgtacc tcttgtctat ttttcatata
    14101 gcacagcgaa tcagaaaaac aggtaattgc ctgatattgt ccgatttctg ataaatcgag
    14161 catattgcct tccacaaatt ggatcgatgc gtcttcttca tatgctcgct ggctggcaat
    14221 catcaacatc tcttctgaaa ggtctaaccc tgtcacttta tatccatctt ttgcaaaggc
    14281 aaccgctaat gctccagtac cgcatgccag ttccaaaata ttttttgtgt cttcttgtaa
    14341 atgacgtttc gaaaaagcta accattgctg ataaagtgtt tcgtccatca cttcatcgta
    14401 aatgaaagca aatgtttcat aagccatatg ttaatccaac cattcactaa gatcgactag
    14461 cggagcatct gaccatagtt tttccagatt atagaaattt cgttctggcg catggaaaac
    14521 atgtacgatc acatctccaa gatcgatcaa tacccatttt ccgccttctt ttccttcaat
    14581 acgttttact tcatatttgt tttcttcttc tttatcaatg atttcttctg taattgcgtt
    14641 gatttgacgt tcgctatttg ctgaacagat catgaaataa tctgccaaca aggaaacatt
    14701 tcgaacgtct aaagcgataa tgtcttctgc ccgttttgaa tctgctgctt caatcgcaat
    14761 ttttaattgt tctttactat ctatgatgat ttcctcctaa tatgagcagc ttattttttt
    14821 gctacccagt aattatatgt ttcgattgtt tttggataaa tctgttgttc ttgcgcaagc
    14881 aaatgggcca atgtatgttt tgtttcaaat gctacagctt cgtctagatc agcccatgca
    14941 atggctcgtg catcttgtac cccaggaaaa tctcgacctg gttcaatata gtctgctaca
    15001 taaatgattt tgtcaaggag actcatctta gcagccccag tcgtatgcac ccggattgca
    15061 tgaagaatct ctgcatcggt gattcctaat tcccgctcta caaacgaagc gccaacaaga
    15121 ccatgccaga ttgcattatt ataattcaat aactccggat caaaaccgtc tcttacgatt
    15181 accattttga actcctcatc tggtcgttct tttgcataat catgtgtcaa tgcagcaata
    15241 ctagcttttt caggagaagc accgtatttt ttggcaagag ccacggctgt ttcttctact
    15301 ccaagcacat gcttgaagcg ttgttcgctc attctcatct gtattttttg catcaggttt
    15361 tctctagaat aagcagtata ttcattacta tagtccatat tcatacaacc cttcattttg
    15421 aatataatca attactttat ctggaacaag gtagcggata gaacagcctt ctttgatctt
    15481 ttgacgtatt ttcgtggaac tgatgtcgat ctcaggtaca tctacccaaa ttacaggata
    15541 tggagtatcc gttgtgtatc ctggtcgacg tatacccaca aagttgacca tagaagtcag
    15601 ctcatcgatt ttataccatt ttggcaaata ttctaccata tccccaccga taataaagta
    15661 ataatctgta tccggattgt tttgcgtcaa ttccttcata gtatcatagg tgtaactttt
    15721 cccaccacga gctaattcaa tcgtttcaat ctgcaaaaaa ggattgtctt ccaccgctaa
    15781 ttccaacatg ttgagtcgat gttttgcatc aatcgtctgt ttctcatcaa catgaggtgg
    15841 ctgataagaa ggcatcaaaa atacgcggtc aagacctaaa ttccggccag cttgttcagc
    15901 catcactaaa tgtgcaaggt gaacgggatt gaatgttccg cccaagatcc ctacttgctt
    15961 tttcttctct aagaattgag gcatttcttc aggaaataat tgtgaacgaa caaatgtctt
    16021 agcttgcgta ttcatcatgg tcactcctta tattgctttt acttcttttg atagcctttg
    16081 atacttttct tttgtggaag gtttgtataa tacaataaca cgtccaatga tttggacgat
    16141 ttcgcaatct atttcttttt ctaatacttg agctgcttct tctgcaatct catctgtatt
    16201 ttgaagtaac gatactttga tcagttctct tttttctaaa gcttcttcaa tttgaacgat
    16261 cattgctgaa ttgatcccac cttttccgat ttgaaaaatc ggctgaagat gatgcgcttt
    16321 gctacgtaaa aaacgctttt gttttcctct taaactcata attttccttc ttccttaaat
    16381 caatgctttg cgtttcaata catctacacc ctttggcgcc cagcccgcaa ttactactgg
    16441 cgctgttaca gtgatccagc ctagaccggc aaaaacgatg tctgtttttt ctttcacaga
    16501 aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca tcaggacgag gaggctgtaa
    16561 taatccacct acatgttttt gataaaattc gtctgctttt tccagtttcg ttcgatgaat
    16621 atttacatca tttgacacat aagcaataaa tgaagcacga ttgccgctga taaaatcaaa
    16681 tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc agctgataaa ctttaggttt
    16741 gatttctttt aacggtgcaa tgatccgcaa atctttttta cctaagtagt gcgccatttg
    16801 atggcgatgg ataattcccg gtgtatcaat caaaaaatgc ccgtcatcta aaggaatctc
    16861 gattttatcc aaggtcgttc ctggaaactg cgaagttgtg attacatctt gtactcccgc
    16921 tgtatttttg atgatttgat tgataagcgt cgattttccg acatttgtta ctccgacgat
    16981 atagacatcc cgtccatcgc ggtattcttc gattttttct aatagttccg tcatctcatg
    17041 agatttctta gcactagtta ataatacatc tactggacga attcctgctt cgtaagcacg
    17101 ttccttcatc cattgtgtta atttagtttt tttcaatgat ttaggtaata tatccacttt
    17161 gttcccaact aaaagtactg gattgtcacc gacaaaacgg tgtagtcctg ggattagtga
    17221 accattgaag tcaaaaatat ccaccacatt gacgatcaat gcatcttttt gccctaattc
    17281 attcagtaag cgcaaaaagt cgtcatccgt caatgagaca tcttgggtct cattataatg
    17341 gcgtaatctg aaacaacgct gacaatagac ttccccggaa gctaaacctt tttccaaagc
    17401 cgtttgcggc gtgtatccta attcaccggg tttgtttgtt tgtataacgg caccacagcc
    17461 gatacattgt aattcttctg tcattcgatt ccacctcgcc agcccatatc agggtgtttt
    17521 gctaatagat aggccataat tcttttttcc caaaaacgat taaattgtgt cttccaacta
    17581 tctgttgcga taatgggttt tacaaggatg ctacgtatcc ctgcacgatt ggctccacgt
    17641 atatcagtca taatctgatc gcctaccatt actatctcat catctgctaa atccaattgt
    17701 ttctttgcta tgttgatccc tcttgctaaa ggtttcattg ctcgtgcaac atacaaaaga
    17761 tcaaattttt caatcgctcg cgcgacacgt tttgaattat tgtttgaaac aacaacaaca
    17821 gggattcctg catttttcat ttctaaaatc caagtaagca actcttctgt tccatctgga
    17881 ttattccatg caatcaacgt attgtctaaa tcggtcaata ctgctttgat gcctagattc
    17941 tttaattggg caggtgtaat ctgatagatg gcttctacca tccatgttgg tttgtaattt
    18001 gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc acataaagta ctcctcaacc
    18061 ccgtattata ctgcatatca gcaaaaaaaa ctacctctat actcgttcgt ctttcagaaa
    18121 aagcatttct ttacaaaatg accaaaaata aagattgatc acagaaaaat ttagaaacaa
    18181 aaaacaacta atattggcgg aacctcttgt atcgtttttc cagtaattta tgctgatcta
    18241 atcgaacaag ttccgtcaat ctttcaatca acgctttttt cataatgtgt gcgactttat
    18301 atcgtggcaa ctgctcgccg ttgaacgttt caggaataac tcgatcaacg attcctaact
    18361 cgcgtagttc tggcgcagta attttcatca actcagctgc ttctgcggcg cgactaccgt
    18421 ctttccacaa aatcgacgca aatccttctg gagaaagcac agcataaatc gtttcttcca
    18481 gcatccagac ttcatccgct aaacctagag caagggcacc gccacttcca ccttcaccaa
    18541 tgatgatggc gatgatcggt acctttaaat cagacatttc gagcagattt ctggcaatag
    18601 cctctccttc gccgcgctct tctgctccta caccgcaata tgcacctgct gtattgatca
    18661 aggtaatcac cggtctattg aacttttccg cttgtttcat cagtcgtaaa gcttttcgat
    18721 agccttcagg atggggagaa ccaaaatttc tgcggatatt ttcctgcaga tcccgtcctt
    18781 tttgaatacc gatcactgtt accggtttgc catctaatcg agcgattcca ccaacaatcg
    18841 cttcgtcatc tgcaaataaa cgatcaccat ggcattcctc gaaagaatcg aagatctctt
    18901 caaaaaaatc caagcttgtc catcgttctt tagctcttgc taactgaacg acatcatgtg
    18961 ctgtcttatc cacgatctat ccatcctttc tgtgtatgca attcaagaag cgtatggatt
    19021 ttctgtttca attccatgcg gggaacaatc tgatcgacaa aaccatgaga taataagaat
    19081 tcagcctttt ggaaatcttc tggaagttcc tgcttgatcg tctgttcgat gacgcgtctt
    19141 ccagcaaaac cgatcaagct ttggggttct gctagaataa tatcgccttc cattgcaaaa
    19201 ctggcagtta ctcctccggt cgtcgggtcc gttaaaacag ttagataaaa aagaccttca
    19261 ttgctatgcc gtttgacagc agcagagatt tttgccatct gcattagaga aaagattcct
    19321 tcttgcatcc gtgcgccacc tgaagctgtg aacaagacga ctggaagact ttcctttgtt
    19381 gctcgttcga ataatctggt gatcttttct ccgacaatcg ttcccatact tcccatgata
    19441 aaattaggat ccatcactcc aataatgaag ggaatcccct gaatggttgc tttaccagtg
    19501 aggactgctt catgaagtcc tgttttatct tgcatttttt taattttttc cgtatattcg
    19561 ggaaatgcta gcggatcttt ggttacaaga tccgtatccc attcttcaaa acttttttca
    19621 tcaataatca aagctagacg ctgccatgca ccgattcgaa aagcgtatcc gcaatgaggg
    19681 catacttttt cttcaccgat gtctttcgta taaaggatat gtttgcaatt tggacacttt
    19741 gcccacatgt tatcaggaac agatggcgag ttagcaggtt tgctctctct attaggattg
    19801 atccgaatat atttcttttt cttaaacaga gccatctatg cgcctcctat ctccgtttct
    19861 ggtgtccaat tcggtaaaaa ttcttcttgt aaaaatgctg tgctgtaatc acctgctatg
    19921 actgcaggat gactaatcaa atccatctga aattctgcgt tcgtgatgat tccttctgtg
    19981 ataaattcac ttaaggctcg ctgcatcttc attaatgctt caaaacgcgt atttccatgg
    20041 acgattattt ttgcaatcat tgaatcataa tatggcggga tcgtatatcc cgggtaaact
    20101 gcactgtcta ctcgcagtcc catcccacct gctggaagca gtaagttttg gatcttccct
    20161 ggagaaggag caaaatggaa agcaggattt tctgcattaa tccggcattc gattgcgtgt
    20221 ccttggaata ctacttcttc ttgacacaca ttcaaaggct caccggctgc tatctccacc
    20281 tgttttttta ctaaatcgat tcctgtgacc atttctgtta ctggatgttc cacttggatt
    20341 cgagtattca tttccatgaa ataaaaatcg ccttcttcat ccatcagaaa ctcgatcgtt
    20401 ccggcatttt cataattgac tgctttagct gctcgaacag cagcgtctcc tagtgcttct
    20461 ctttttgttt gtgagatcac tacagaagga gattcttcca gtactttttg attgttgcgt
    20521 tgtaaagaac aatctcgctc gcctaaatga atgacatgac caaatcgatc acctaagatc
    20581 tgaacttcta tatggcgagc aggataaatg attttttcca aatacatatc atcatttcca
    20641 aatgccgctg ctgcttcttg ctgagctgaa gaaaaatgct gtgggagctc ttcttttgat
    20701 tgaactttcc gaatgccttt cccgccacca cctgcagctg ctttcagcat cactggataa
    20761 ccaattctgt ctgctatttc caatgcttct tctactgtag ataatacacc agtacttcct
    20821 ggaatgactg ggacatttgc ttcttgcatc aattgacgcg cattgatctt atttcccatt
    20881 gcatcgatcg ttgcactttt aggaccaata aatgtgatat tgcactcttc acacatggag
    20941 gcaaatcggc tattttcaga taaaaaacca aatcctggat gaattgcttc ggcttttgtc
    21001 acgattgcag cgctcagtac ttcctgaaca ttcagatatg aatcaacggc ttttgccggg
    21061 ccaatgcaga ttgcttcatc tgctaactgt gtatgaagtg cttcggcatc tgcttctgaa
    21121 tatatcgcta cggtttggat ccctagttcg cggcatgcac gaatgatacg tacagcgatt
    21181 tcaccgcggt tagctatcaa tactttagaa aacatatttc acctatccaa tcataaaggt
    21241 taattccgct tcggctactt ttttaccgtc tactttagcg attcctttgc ctattccggc
    21301 tgaagctttc actttcaata tctcaacttc aaggtataag gtatctcccg gtgtaacttt
    21361 cttacggaat tttgctttgt ccaatccgcc gaaataagcc gtctttccct tgaattgttc
    21421 aagagaaagt aatgcaactg ctcctgcttg agccattgct tcaacaatca atacccctgg
    21481 cataacaggt tcttctggaa aatgtccttg aaaaaagggc tcattgatcg ttacattttt
    21541 cttagcaacg atccgttctc cttcaaccat ttcttccact cgatcaatca aaagcattgg
    21601 ataacgatgt ggaatgattt cttttatttc ttgtatgttc attgttttcc ctctttcctg
    21661 ctttgcttat ttttgctgaa tcctaaagag tggctgtcca tattcgacaa ctgactcgtt
    21721 ttctacaagg atttctgtga tcactccgtc ggcctcacta gttatctcat tcatcagttt
    21781 catcgcttca ataatgcaga taacttctcc tttggcaact tgatcaccta cttttttgaa
    21841 agctggtttg tcgggcgctg gttgcagata aacgattccc acgattggcg aaacaacctc
    21901 gattccatca aatactgccg attctttttc tttcattatt tctggagcag ttgttgcttg
    21961 ttcaggtatg acattgactg gctccgaaac tggcaacgtt tcgtttgttt gaaccatatt
    22021 catgcctcga cttgttttat ttttattcat atacaattca aattgacctt cacgcaaatc
    22081 gaattccgtt agcgaggatt gatcaaattg actgactaat tctctgattt cattgatatc
    22141 caaaattatt cctcccagcg tttcatgcac agaaccgcat tatgtccgcc aaaccctaat
    22201 gaattactca ttgcataagt aaaacttgca ggtttgcttt cgtttttgac aactgtcaag
    22261 tcaatctcag gatcttgttt ttctacattg atcgtaggtg ggataaattg atgttctaat
    22321 gccagcaaag ttgccaaggc ttcgatcccg ccggctgctc ccaataaatg tccagtcatc
    22381 gatttcgtgc tgctgacgta agtatctctg aaattttcac ctaatgcgat ttggattgct
    22441 tttgcttctg cctggtcatt ggctggtgta cttgttccgt gagcattgat atatccgact
    22501 tgttccggct gtacaccagc ttcttccatc gcaagcttca ttgctttaga tgcaccagaa
    22561 ccatctggag taggagctgt catatggtaa gcgtcacagt ttgcaccgta tccaacgatt
    22621 tctccataga ttttggcacc gcgagcttgt gcatgttcta aagattcaag aactagaacg
    22681 cctgctcctt cacccatcac aaaaccattc cgattttcgt caaacggaat cgaaccacga
    22741 ttaggatcag ttgattctga gagtgctgtt aaagctgcaa atccagcaat ccccatctca
    22801 caaatcgttg cttctgttcc tccagcaaga atgacatcag aatagccatg tttgatgtta
    22861 cggaatgctt ccccaattga attcgttccg ctagcacaag ctgttacgat agctgtacaa
    22921 ataccttttg cacctacacg caaagcaata tttccagctg ccatgttggc aatcgccatt
    22981 ggtacgaaca ttggagaaac tcgttttgca cctttttcat gtagacgaat aacttggttt
    23041 tcgattgttg gaagaccacc gatcccagat ccgatgatga cgccaaaccg gtcttgattc
    23101 attttttctt tatctaaacc gctcatctcc attgcttcta aagcagcgta gattccatag
    23161 atcgaaaaca tatccattcg tttgctatct tttttaataa aatatttatc gtaagggaaa
    23221 tctttgactt cacccgctaa ggtaatccct gtttctgaag catcaaattt agtgatcggt
    23281 gcgatgccat tcgtcccctc ttttaagcta ttcaaaaagc tgtctgcgtc atttccaata
    23341 ggtgaagtga ctccatagcc agtaattacc actcgattca tttgtttgct ctccttttct
    23401 tatccgtgca tgaccaatcc gccatcgaca ttgatgactt gtccggtgat atatggattt
    23461 tgtgctaaga aaactgctgt ttgtgcaata tcttctactt gtccgaaacg ctgcatcgga
    23521 atttgctttt ccgcttgttc cttgactttg tctgctaaaa cttccgtcat atcagttgtg
    23581 atgaatcctg gcgcaatggc attacatgta atgcctctag ttgcagcttc tcgtgccaca
    23641 gattttgtta atcctactac gccagcttta ctagctgcat aatttgcttg accaatattt
    23701 ccaatcaacc cagatacact agagagatta atgatggccc cttctcgctg cttcatcatt
    23761 tttttcaata cgtgctgcgt catattgaag gttccaatca aattgatatc cagacatttt
    23821 ttgaaatctt ctgcatccat tcgcatcaca agtttgtcat ttgtgattcc ggcattgttt
    23881 actaacacat ggatcgatcc aagcttttct tctgcttctt tgatcatttg tcccgctttc
    23941 tcataatcag agatgtcacc ggaaactcct acgcatttta caccgaatgc ttcgatttct
    24001 tctatctttt cttttggaat ctctccgcga ccattcaaaa tgatatttgc tccagctttt
    24061 gcaaaagcta aggccatcgc tttgccgatt cctcttgtac tacctgtgat aaatacattt
    24121 tttcctttta catccataac gttcctcctt atagtattgc tattgtttca tcaaatgtct
    24181 gcttattctc aacacgcaga actggtattg ttttatcgat ttttttcata aatccgctta
    24241 atacttttcc ggggccgact tcgatgactt gttcgatgcc gattgttttt aacttatgga
    24301 tactctcata gaaacgaaca ggttgcatga cttgctgttc aagtagtccc gcgattgttt
    24361 ctttcttcat tatttccgtt gttgtgttgc ttatgatagg aaaagaaggt tcagagaatt
    24421 gaatctgact taggtccttc gccagttttt ttgctgccgg ttctaaaata gctgtatgaa
    24481 aaggcccgct gacatttaga gggatcattc ttttgaaccc tttttctttt aatagtaaaa
    24541 cagcttcgtc tactgctttt tcttcgccac caatcacgat ctgctgaggt gtattataat
    24601 ttgcaggaga tacgatgccg tacttgctag cttcatgaca gctttcctcg atcgtttcaa
    24661 tcggcgcatt catcactgca accattttcc cgcttccagc tggggcagct tctgtcatat
    24721 aagcgcctct ttttgctact aacgcaactg cttcagtgaa ggatagcgca ttactagcta
    24781 ctaatgccga atattctcct aaactcagtc ctaaagctgc atctgggatg attccatgct
    24841 ccttcaataa gcgataatac gcgatgctga ctgttagaat agctggctgt gtatattgcg
    24901 tctgatccaa acgttcattt tctgtaaagc aaagttcagc catatcgtag ccgagaatct
    24961 cacttgcttc atcaaatgtc tgctttacaa cggcttcttc atataaatct tttcccatac
    25021 cttgatattg tgcaccttgg ccactaaata agaatgctgt tttcatgttt ttctccaata
    25081 tatccagaat atatgtttga ttcttcctat tcaaaaaggc cgagacattc ctatttcagg
    25141 aaatgtccca gcccccaacc taattttaat gattatgcag tttgtttttc tacgtaagtc
    25201 actaaatctt gaaccgtttt gattccgtct tctgtttcga ttttcacgtc aaattcatct
    25261 tcaatttcat tgatgatttg gaataaatcc aagctgtcag cttctaagtc ttcttggatg
    25321 tcagtagtca atttgatttc ttcagtttct tttcctaatt cttctgcgat gatttcttga
    25381 attttttcga ataccataat aatatattcc tccgatattg tttgcttttt atttttataa
    25441 tttaacagtc tacaactcca tgagaatact tccccatgtt aaacctccgc catagcctgt
    25501 aaatattact ttctgctgtt ttcctaaggt gatctttcca ctagaaactg cttcatccaa
    25561 taagataggg atgcttgcag cagatgtatt cccatattta tccatattgg tcaggaactt
    25621 ttcttgcggc atctttactt ttcgggcaat tttctcaata attctgaggt ttgcctgatg
    25681 caaaagcaga taatcaactg gtttgtcttt catgacttct tggatattgt ttgcaacgtc
    25741 tcgtacggca aaatcaaaaa tatctctgcc agtcatttgc aaataatacg aaccttctga
    25801 atcttctgaa tagaacggat ttttattttg ctggtagcca gcggtcaatg agctacttct
    25861 ggtaccgtca gctgctaatt tttctttcag caattttttc tgtggattcg cttctaagag
    25921 aatcccacct gctccatcgc caaacaacac agcagtcgta cgatcagacc aatcgattag
    25981 cttagatagt gtttcaccac caatcaccag tcccttagtt gagccgcagc ggatcatttt
    26041 ttcagccatg cttaacgcat aaacaaatcc tgagcacgca gcactgatat caaaagcgat
    26101 agcatgagtt gctcccaatt tcccttgtac ttgtgctgct acagatggtg tcgtgtaatc
    26161 gggcgacatt gttgctacta aaataaaatc caaatcttta gggtttagat ttttctgttt
    26221 tatcaatttt tccgccacta aaatgcataa atcagaagtc tcctgatttg ttacacatct
    26281 tcgttcacga atacccgtcc ttgacgaaat ccattcatca gaggtatcca tcatcttgga
    26341 aagatcatta ttagtaataa tcttttcagg aaggacgctg gctgttgcag taatcattcc
    26401 aaattgttcc attaaatatc ctcacttata gtcttgcaag aagtcgtgca agtttttcag
    26461 tgcatccagc aaggcatgct gttcctccgt agccattccg tctaagatgt tcttgaccat
    26521 ttcacgatga aaatgctgat ggacccgaaa tagtaacttt ccttttttcg ttagtcccaa
    26581 ctttacaacg cggcggtcat cttcactgcg gatacgttcg acatagcctt tttttactaa
    26641 cttgttaata gcagtggtca aggtcccaac agtaattgat agttctttgg ctacttctga
    26701 agttgttttt ttcttgtaca tcccgattgc ctcgatcgtg tgcatctctg tgattgacag
    26761 atctttgaat tgagattttt tcagctcaga ctcttcgatt gtcaaaatat cattgaagac
    26821 actaacaaga taatcattta ctgtttttaa atctggttcc atgttccacc gtccactaat
    26881 tagtttgatt atcaaatctt ttgatgttca aactatatct taactttttt caaaatgcaa
    26941 ccttaaaaga tcattttttt actatatgag ctttttttaa
//
LOCUS       DAZKNH010000030        26370 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000030 DAZKNH010000000
VERSION     DAZKNH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.030"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..611)
                     /locus_tag="ACG8MG_001201"
     CDS             complement(243..611)
                     /locus_tag="ACG8MG_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFQ0375258.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            761..1243
                     /locus_tag="ACG8MG_001202"
     CDS             761..1243
                     /locus_tag="ACG8MG_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFQ0375259.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1280..2290
                     /locus_tag="ACG8MG_001203"
     CDS             1280..2290
                     /locus_tag="ACG8MG_001203"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFQ0375260.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2405..3118)
                     /locus_tag="ACG8MG_001204"
     CDS             complement(2405..3118)
                     /locus_tag="ACG8MG_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375261.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3291..3898)
                     /locus_tag="ACG8MG_001205"
                     /pseudo
     CDS             complement(3291..3898)
                     /locus_tag="ACG8MG_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            4382..4609
                     /locus_tag="ACG8MG_001206"
     CDS             4382..4609
                     /locus_tag="ACG8MG_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFQ0375262.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4625..5791
                     /gene="hemW"
                     /locus_tag="ACG8MG_001207"
     CDS             4625..5791
                     /gene="hemW"
                     /locus_tag="ACG8MG_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFQ0375263.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6025..7068
                     /gene="hrcA"
                     /locus_tag="ACG8MG_001208"
     CDS             6025..7068
                     /gene="hrcA"
                     /locus_tag="ACG8MG_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFQ0375264.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7162..7725
                     /gene="grpE"
                     /locus_tag="ACG8MG_001209"
     CDS             7162..7725
                     /gene="grpE"
                     /locus_tag="ACG8MG_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFQ0375265.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7776..9605
                     /gene="dnaK"
                     /locus_tag="ACG8MG_001210"
     CDS             7776..9605
                     /gene="dnaK"
                     /locus_tag="ACG8MG_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFQ0375266.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9756..10922
                     /gene="dnaJ"
                     /locus_tag="ACG8MG_001211"
     CDS             9756..10922
                     /gene="dnaJ"
                     /locus_tag="ACG8MG_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFQ0375267.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(11004..11633)
                     /locus_tag="ACG8MG_001212"
     CDS             complement(11004..11633)
                     /locus_tag="ACG8MG_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0375268.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            11645..11818
                     /locus_tag="ACG8MG_001213"
     CDS             11645..11818
                     /locus_tag="ACG8MG_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375269.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11863..12600)
                     /locus_tag="ACG8MG_001214"
     CDS             complement(11863..12600)
                     /locus_tag="ACG8MG_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375270.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12602..14104)
                     /locus_tag="ACG8MG_001215"
     CDS             complement(12602..14104)
                     /locus_tag="ACG8MG_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0375271.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14363..16357
                     /gene="uvrB"
                     /locus_tag="ACG8MG_001216"
     CDS             14363..16357
                     /gene="uvrB"
                     /locus_tag="ACG8MG_001216"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0375272.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            16367..19183
                     /gene="uvrA"
                     /locus_tag="ACG8MG_001217"
     CDS             16367..19183
                     /gene="uvrA"
                     /locus_tag="ACG8MG_001217"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0375273.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19446..20711
                     /locus_tag="ACG8MG_001218"
     CDS             19446..20711
                     /locus_tag="ACG8MG_001218"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFQ0375274.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20715..21446
                     /locus_tag="ACG8MG_001219"
     CDS             20715..21446
                     /locus_tag="ACG8MG_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFQ0375275.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            21581..22465
                     /gene="rapZ"
                     /locus_tag="ACG8MG_001220"
     CDS             21581..22465
                     /gene="rapZ"
                     /locus_tag="ACG8MG_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFQ0375276.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22462..23460
                     /gene="yvcK"
                     /locus_tag="ACG8MG_001221"
     CDS             22462..23460
                     /gene="yvcK"
                     /locus_tag="ACG8MG_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFQ0375277.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23494..24429
                     /gene="whiA"
                     /locus_tag="ACG8MG_001222"
     CDS             23494..24429
                     /gene="whiA"
                     /locus_tag="ACG8MG_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFQ0375278.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24972..25622
                     /locus_tag="ACG8MG_001223"
     CDS             24972..25622
                     /locus_tag="ACG8MG_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375279.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            25809..>26370
                     /locus_tag="ACG8MG_001224"
     CDS             25809..>26370
                     /locus_tag="ACG8MG_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFQ0375280.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         8708 a         4450 c         5602 g         7610 t
ORIGIN      
        1 attttttcct tattcaaaag ttttgcagga aaattgctga aaaaataatt ttactaagta
       61 gaaaattcaa agccattcaa tcagtgattt ttttcagcac aagaaagact ccttataaaa
      121 atagcagata ctttgtcaat taagtgttta accagggatg ataaaaagga taggattgaa
      181 caaaacctgt ataaggtgat gaatagtttc ctcgccagta aaaggtttat tcgtataaag
      241 ctttatcgcc aaaatttatc atgacctttt ttatcttcaa tattaaatga aatcaattgt
      301 tcaatcaatt cccagttgat tacacttttc caaggaactt tgatgacgtt atccgtatat
      361 acgtagcctg cttcttggat atttttttca agggcacgaa tcgctgcttt ttccggtgcg
      421 atagaaaaat gatttttagc aaaactaaat gcaataataa aggtaccatg gtcagtaaac
      481 ataggttggt tccattttac agttgtgtct agctggggat acgtttctgt tatcttagca
      541 aaaaggtctt tgatcttttg ttgatgttct ttttctttga tttgattgat gatcggctga
      601 aatttttcca ttctaaaacg cctccattaa gtggtttgat ttattttacc agaaaaacaa
      661 tagttatgta taaaaaacga acaaaacaca agtaatgttg taaccgctat atttatatga
      721 taagatgtct caaataataa aatagaaaag agacataaaa atgattgact tggcaaagaa
      781 attcttacac gaaaatatat attttgagtt gtatcatcat cgagccattt atacaaatga
      841 agatgctttg gtaataaaga aagaacaagg atttacaggt acagagacca aaagcttatt
      901 cttaaaagat aaaaaagata ataactacgt atatctgact tttactacga aacaaacgga
      961 tttcaaagca ttagggaaga tcgtcggtaa aaggctttct attgtttcat cagaacgcat
     1021 ggaagaacaa acgggacaaa agcagggcgc tgtttctccg tttggttatg aaaaacagat
     1081 tccgattatt attgatgaag agctcttatc aaatgaaaaa cttgtttttg caccaggtag
     1141 accagatcaa acgatggtca tccaaacggt tgacttaaac aaaatcatcc agctacttga
     1201 gatgccgata tttttgatgc cggcaaacga aaaaaacaat taaccaagta aaatagtgca
     1261 tacttggagg tgaagcaaca tgataagtta tccattatta gaaagaaatg caacgattgg
     1321 ggttactgca ccatcctcag gcgtatcaga ggaacaccat tcgttactcg aatcagcttt
     1381 aaagagaatg gaagaaaaat ctttccaagt agttactgga aagacgcttt ggacgcaaga
     1441 taaggaaaaa tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa tgatgcaaga
     1501 tgaaaatatc caaatgatct ttccgacttg gggaggagaa ttattgattg agattctcga
     1561 ttttgtcgat tttgatgctc tacaaccaaa atggatagta ggttattcag atattagtac
     1621 gttgctactt gctgtcacat taaaaacagg gatagccact gcacatggaa ctaaccttat
     1681 tgatttaaga ggagagtact tggatgaaac gacagcaatg tgggaaagtg tactgaaaac
     1741 aagagaagga gaatcgattc tacagcaatc ttctgaaaag tatcaaaaag tgtggcccca
     1801 tgagcattca tcatcttctg ttttccatct cactgaacca acaaattggc aatcagtcag
     1861 cggagatgca gtaaggatga agggacgatt attaggtggt tgtattgata cgatcaggca
     1921 tttaatcggt actccttatg gagatgtcca atcttttaaa agaaagtata atgatggaaa
     1981 gccgatcttg tggtttctag aaaactgtga gctatcgtca gttgatttaa gaagatcact
     2041 tgttcagatg aaactggcag gctggtttga tggcagctcg gggatcatgt tcggtagaag
     2101 tgcggcagag gatttgttag attataaaat gaaggatgta tacgaagatc tgtcaaagga
     2161 attaaaacta ccgatcttgt atgatattga ttgtggtcat gtaccgccac aactgacatt
     2221 gattaatggt gcctatgcag aagtagcggt agaagataga aaagcgactg ttttgcaaac
     2281 gtttatttaa aaaatttttt cctgccttgg agtctatcaa agaccaataa atctaaatag
     2341 aaaagaaagt gaaagaagct gacaaaatgc tgtcagcttc tttttgtata acatattaga
     2401 aagtctaatt atctataact gtcacatcga ttaactgttt cacgtcattg ggatttcctg
     2461 taggaatagc tactacagat gcattatcaa tgatttcttg agttgtttct ctaatgaacg
     2521 ctggataaga tgtttgatct gcaggcattt gccaatgtga aggaactgga taactagtga
     2581 ttgtcccgtc atggttactt gaatacacta aaactccctg gtaactgtat ttcccagtga
     2641 gcataaccac atctttcgga tagactgcac tccctgcatc ataaggggcg ataggtgttc
     2701 ctgcaggata atgaataaca tgtaattcga aatcacccga tgtatctcta aaagtattac
     2761 ctctaatagc tagccaaaca cgtgcgtact caatttgtag atcagaatat cctgctaacg
     2821 gatctattga ctgagaagac tgtgaggagg ttgtgtctat tgttgaagaa gaaatatttg
     2881 tctcaactgt actactagat gtagaagaag tattgggctc tgagacttta ctgcttgttg
     2941 aagatgtatt cgtttcagta gaagctgatt tctgagtact tgatttttca gctgaactgg
     3001 tttgtctgat tgaacttttg tctgtggtcg cagatggatc actattttct ttactaggag
     3061 acgtacatcc tacaaggaat aaagagacca acaataaaat aaaacctaac tttttcatat
     3121 aaaatacctc attatttttt taataccagt atagcataat cctgaatttt tgaatccgta
     3181 aaaccttgtc tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt
     3241 gacaaataat catcagtaaa attacttgtc acactattga taaggagtat ttaattgaag
     3301 agaatgatta ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt
     3361 atagacgaat cgttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca
     3421 agtctgtgaa ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag
     3481 cttgttgttc tataaaatct tctttattct gataattttc cgattctaac caaggaaatt
     3541 gcgcatttcc ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg
     3601 ccccacaaat agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata
     3661 aatgatgatt ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt
     3721 caggtatttg ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct
     3781 ctgattggat agaagcagtt ttgttttgcc atttctcatt ttggtttagt gtgctagtaa
     3841 tataagcacc ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt
     3901 tcaagctctc ctttactatc tataacaagg acaacagttt taacctacag acattcatta
     3961 aaattgggta gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagatag
     4021 tcaacttaat taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca
     4081 ttataacatt ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat
     4141 attaaaagaa actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc
     4201 actcagacat gaaaacaacc ctagagatat ataactaggt aaccagttta atgaaagaaa
     4261 aaatcatcca agaagtcgat tcttggattt tttagtggat ttgttcgcca aatacgttta
     4321 gtatgcgtat atgagaggtt gtaagcaaag cgttgtggtc tgagcactta aggaggaatc
     4381 tgtgaaaata gcttttcata tttttgcaga ttttgactta atgccgaagg tcagctttgc
     4441 gattaccgtt tatcgagagg ctgtaaaagc accgtgtagc tccgatgaca ataagcaagc
     4501 atttttagag ctaggtttcc ttgattctaa atatgcggct tatttcaatg agttggtgct
     4561 tgaacgcgat tttaagaagg tcgaaaacag gcacgacttt tcgacgtaag gagaaagaat
     4621 ttcaatgata gaaacaataa atcccgcaga aaacaaggtt tctgcgtaca ttcatatccc
     4681 attttgcgag catatctgct attactgtga ttttaataaa gtttttttag aagggcagcc
     4741 agtagatgag tatgtggaaa tgctgctgaa tgaaatgaaa ataatgctag aaagtcatcc
     4801 gacagcgaat ttagaaacac tttacgtagg gggcgggaca ccaacttctt tatctgctaa
     4861 acaacttgat cgactgttat caggtgctag agagatcctg ccttttcaaa atggtaatga
     4921 gtttactgta gaagcgaatc caggtgattt gacccgtgaa aaacttcaag taatgaaaaa
     4981 ctatggcgtc aatcgtttat ccatgggagt gcagacattt gataatcgct tattgaaaaa
     5041 aattggacgt aaacacaccg cagaagatgt gtatcaaact atgcaatttt tagaagcaga
     5101 aaattttacc aatgtaagca tcgatttgat ttatgcatta ccgggacaaa cactagaagg
     5161 atatctggat actttggaaa aagctttggc tttggatctg cctcactatt ctttgtactc
     5221 attgatcctc gaaaacaaaa caatgttcat gaactgggta cgacaaggaa gactcgaact
     5281 gcctgatcag gaaacggaaa ccagaatgtt tgaagagacg attgaagcaa tggaaaagca
     5341 tggacgtcac caatatgaaa taagtaactt tgggctctcc ggacatgaat ctcaacataa
     5401 cctcatgtac tggaacaatg cccactattt tgggtttgga gctggtgcta gcggttatct
     5461 tggagatatt cgttaccgca acaaaggacc aatccaacac tatttacgcc cattacgagc
     5521 aaatgaacgc ccggtattgg aagaagaaaa attgacacga aaaaatcaaa tagaagaaga
     5581 aatgttctta ggactaagaa aaaaaacagg gattactaaa aaacatttct atgatcgcta
     5641 tcaaaaaaca attgaatcgg tgtacggtga tgtgctaagg gaattagagc aagaagggtt
     5701 acttgttaat gaagaagacc gtgtttattt gacaccaaga gggacctttt tggggaacaa
     5761 tgtgtttgag cgctttttgt ttgaaaaata gtttaaagaa tatttgtttt ttatctgaat
     5821 ttaaataaaa aaagttcaaa aatacaataa aggactattg aaacgttgat ataactggat
     5881 ttacatcaat tatttagaat tagcactctt gtgtttagag tgctaaaaaa tggagtcttt
     5941 ctcttgacag gagaagggct tcttgctata tttataagtg gattagcact tagaatatat
     6001 gagtgctaat gagaggtgag gaagatgtta acaaagcgac aaaacgacat tttacgtctg
     6061 atcatccaac actatacgag tagtggcgtg cccgttggtt ccaaaacatt gatgtctgag
     6121 ggagtcaagg ccagctcagc aacgattcgc aatgatatga aagcattaga agatgaagga
     6181 ttgctgttga agactcactc ttcttctggg cggattccat ctgctttagg ctatcgttat
     6241 tacgtggatc atttgttaaa acctgctcgc gtagcagaag atgatttgct gctgattcgt
     6301 cagtctctag gcagagaatt tcatgaaatc aatgagatta ttaagcaatc tgctgagata
     6361 ttatctgagc tgacaagcta tacgactttt tcattgggac ctgaaataaa agaccgccga
     6421 ctgactggct ttcgtatcgt acccttgaat aatcgacaga ttattgctat cgtcgtaaca
     6481 gatatgggga atgtagaaag ccaagtgttc actcttcccg aatatatggg aagtcaggaa
     6541 ctagaaaaaa tggtacagat tgtaaacgac cgtctagtcg gagatcctct gatgacggtg
     6601 taccataaac ttcgtacgga gattccgatg attcttcata aatatttcca aacgacagaa
     6661 gggatgtctc agttatttga caccgttctt ggtcatgttt ttgaagataa ggtttatgtc
     6721 agtggacaaa tgaatctctt ggattatgag ccccgtcaag atcttgatca gttcaaatcg
     6781 atgttttctt ttatgaaaga ctcagacgga cttacacaaa tgatcactcc gatggatagc
     6841 aatattcata tcaagattgg tgctgaatta ggcaatgatt tattgcagaa tatgagtatg
     6901 attcaagcaa gttacgagat agatggtcat ggaagaggta caatagcctt gttagggcct
     6961 actagtatgc cttattctcg gatgttcggt ctagtcgatg tttatcgaag agaactagca
     7021 gctaaacttg ctgattacta ccgttcgctt gacttgtcaa actcgtaatg tgaataacga
     7081 agctatttgc agctgatcgt tctctctttc ttagtaagag caatcaaaca gctgactgaa
     7141 taatgaaagg aagactgtat cgtgaaagaa aatcaagaag aactagataa agaaatgaca
     7201 gatgctcagc cagaaccgga aatcgatgtc gaagctgcag aagattcggg tatttcagaa
     7261 gctgaagcgg aagaattcga aacagcgaaa ttaaaagctg agttagaaga aatggaagat
     7321 aaatacttgc gggctcgtgc tgaaatcgca aatatggcaa atcgaggaaa aaatgagcgt
     7381 gagcaattac aaaaataccg ttcacaagat ttagccaaaa aattacttcc atcaattgac
     7441 aacctggaac gtgcattagc cacagaagtg tcagatgatc aaggggcagg tttgaaaaaa
     7501 ggcgtggaaa tggtcttgga aagtttgcgt aacgcactgg aagaagaagg aattgaaaaa
     7561 atccctgcta aaggagaagc ctttgatcct aatcttcatc aagcagtaca aactgttcca
     7621 gcgactgaag atacaccagc cgatacgatt gtagaagtac tacaagaagg ctacaaatta
     7681 catgatcgcg tgttaagacc aaccatggtc attgttgcac aataattgtg cggatataaa
     7741 acttacactt tcaattaaag aaggagacgt taattatgag taaaattatc ggtattgact
     7801 taggtacgac taactctgca gtagcagtat tggaaggaaa cgaagcaaaa atcatcgcta
     7861 acccagaagg aaaccgtaca acaccttctg ttgtatcatt caaaaatgga gaaatccaag
     7921 taggggaagt tgcaaaacgt caagctgtga caaacccaaa tacgatttca tcaatcaaac
     7981 gccatatcgg tgaagcaggt tacaaagtag atgtagaagg taaatcatat acaccacaag
     8041 aaatctcagc aatgatcctt caatatatca aaggattcgc agaagactat ttaggtgaaa
     8101 aagtcgaaaa agcagttatc actgtgccag cttactttaa tgatgcacaa cgtcaagcaa
     8161 caaaagatgc tggtaagatc gctggtctag aagtagaacg tatcgtcaat gaaccaactg
     8221 cagctgctct tgcctatggt ttagataaaa cagatcgcga cgaaaaaatc ttagtattcg
     8281 accttggtgg cggtactttt gacgtatcta tccttgaact aggagacggt gtctttgatg
     8341 tattgtcaac agctggcgac aaccatctag gtggggacga ctttgataac aaaatcatcg
     8401 actacatggt agcagaattc aaaaaagaaa acagcattga cttgtctcaa gacaaaatgg
     8461 ctttacaacg cttgaaagac gctgctgaaa aagcgaaaaa agatttatct ggcgtatcaa
     8521 gcacacaaat cagcttgcca tttatcacag caggagaatc tggaccattg catttggaaa
     8581 tgacattgac acgtgcaaaa tttgatgaat taacagctga cttagtagaa cgtacaaaag
     8641 taccagtacg tcaagcgcta aaagatgcag gtttatcaca atcagaaatc gacgaagtta
     8701 ttttagtcgg tggatctact cgtatccctg cagttgtaga agcagtacgt aaagaaacag
     8761 gaaaagaacc aaacaaatca gttaaccctg atgaagtagt tgctatgggt gctgctatcc
     8821 aaggtggcgt gatcactggt gatgtgaaag atgttgtttt acttgacgtt acaccattgt
     8881 cattaggtat cgaaacaatg ggtggagtgt tcactaaatt gattgaccgc aatacaacga
     8941 ttccaacaag taaatcacaa gtattctcaa ctgcggcaga taatcaacca gcagtagaca
     9001 tccatgtatt acaaggtgaa cgcccaatgg ctgctgacaa caaaactcta ggaagattcc
     9061 aattgacaga tattccagca gctcctcgtg gtattccaca aatcgaagta acattcgata
     9121 ttgataaaaa cgggattgta aatgtatctg cgaaagatct aggtacacaa aaagaacaaa
     9181 aaatcacgat caaatcttct tcaggtctaa cagacgaaga aatcgaacgt atggtaaaag
     9241 atgcagaagc aaatgcagaa gcagataaag ctcgtaaaga ggaagttgac ctacgtaatg
     9301 atgtagatgc attgctattc tctgtggaca aaacattgaa agaattagaa ggcaaagtgg
     9361 acgaagaaga agtgaaaaaa gcagaaacag ctcgcgatga attgaaagcc gctgttgaag
     9421 caaacaacat cgaagaaatg aaaactaaac gcgatgcatt gaacgaaatc gttcaaaact
     9481 tgactgttaa actatacgaa caagcggcac aacaacaagc tcaagaaaac ccagaagcag
     9541 ctcaaggcgg agcagatgat gtcgttgatg ctgattttga agaagtcgat ggcgacgata
     9601 aataatcagt tcactgaatt tcgagagaag gccaaagttg ctactttggc tttttctttt
     9661 gaaaaatagt ttgaagtatc ggtttttgtt tcaaacaaaa tatgatatga taacaagcga
     9721 cgtgaaaatg atgagaacca ataggaggaa agcctatggc aacaaaacgt gattattatg
     9781 aagtcttagg gctctcaaaa ggagcatcag aagatgaaat aaagaaagct tatcgtaagc
     9841 tttcgaaaaa ataccatcct gacatcaata aagaacctga tgcggaagaa aaattcaaag
     9901 aagtatcaga agcatatgag attttaagtg acccgcaaaa acgcgctgca tatgaccaat
     9961 acgggcatgc aggcacagat ccaaattacg gtgctggtgg agctggtggc ggatttggtg
    10021 gattcggcgg cttctctggt ggcggcttcg gtggttttga agatatcttc gactctttct
    10081 ttggaggagg cggtcggtca gtcgatccaa atgcgcctcg tcaaggtgct gatttgcaat
    10141 atacggtcga cttgtctttt gaagaagcga tttttggtgt agaaaaagaa atcaagtata
    10201 atcgggaaga aatctgtcat acttgtggtg ggaacggtgc aaaaccaggc acacaaccaa
    10261 ctacttgtca taaatgtcat ggtgcaggta cgattaatgt agaacgccaa acaccacttg
    10321 gacgagtgat gagccgtcaa acttgtgatg tttgtcatgg gacagggaaa gaaatcaaag
    10381 aaccttgtcc aacctgtcat ggctcaggac atgagaagaa agcacattca gttaaagtga
    10441 atgtaccagc tggtgtagaa gaaggacaac aaatgcgttt agctggtcaa ggagaagctg
    10501 gagaaaacgg cggaccattt ggtgatttgt atgtggtctt ccgtgtagaa gaaagtgata
    10561 tctttgatcg tgaaggttct gaaatttact atgaattgcc tttaagtttt gtacaagcag
    10621 ctttggggga tgaagtccaa gtaccaactg tccatggaga tgtgaaattg aagatccctg
    10681 ctggaacaca gacaggtacg aacttccgtt tacgtggaaa aggcgctccg aagttacgtg
    10741 gtggtgcaac aggcgaccaa catgtcaaag taaaactgat cacaccgaaa aatctaaatg
    10801 aacaacaaag agaagcatta cgtgcattcg ctgaagctgg cggcatcaaa gttgaagaac
    10861 agcaagaaga cggtttcttc gataagttta aagatgcttt tggcgggaaa aagcggaagt
    10921 agtttttcta gtaaaaacgc taagtcatta ataattaaaa accagacgaa caatcatatg
    10981 ttcgcctggt ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag ctttagaata
    11041 tgataatcgt catagtttct aggggtataa ccagtgagag aatggaactt gttcaatcga
    11101 tattgcaagg tgtttttatg aataaataat tcttctgagc aatttttgat gcttccgtta
    11161 tttttactga agcatgccaa agtcttcttg aacaaatgta cttcttcttc tgtcaggctt
    11221 ttcaaaatac gttttacata taatgttcgt tgatctgacg ggattgctgg taaaacgatc
    11281 ccgaggtcta aatcttcaaa aaagcggatc tcgttcacag attgatggaa agctatccat
    11341 tcgcaaactt gtttggcatg aaggaaagat tgcggtgtct cttgctctgt ataacaaagc
    11401 gacccaattc caaagtttag ctgtaactga tacattttat taaactttgt ctggatttta
    11461 tcaagcaatg atagcaatga ggctctggaa atccgattga ctaggataat gatttgttgt
    11521 ctatgtacag tgagcaaagc ctgtccgtaa taagagaaga ccctttcgag caactccaat
    11581 aaaggattga tccgttcttg acacaattgc tgattgtcag aaagttgtcc cacaactaca
    11641 taatatggag aagaaaaatc aagctcaatt aagtgagggt ggtatcgact atgtacttga
    11701 tatcatcaat gaaccgatgg tactttctta tgcgatggaa caggtagagc gattgcttgt
    11761 ttgtgcagga aaacaagcga tgcggctgat acgattaggt gagaaaataa gaagataaaa
    11821 atacacggtc ccttcatggc gaccgtgcat ttttggtttt tattaaatat ttaatacttt
    11881 attcaagaaa tcttttgtcc gttcattttg cggattttca aagatttgtt gaggcgttcc
    11941 atcttctaaa aagtttccgt cgtcaatgaa catgacgcga ttggccactt ctttagcgaa
    12001 ccccatttcg tgcgtaacga taaccatcgt cataccttgt tctgctaact ttttcatgac
    12061 gttcaagaca tcaccgacca tttcaggatc gagtgcagaa gtaggctcat caaaaagcat
    12121 gatgtcaggg ttcatcgcta gagcacgagc aatagccaca cgctgttttt gtccaccaga
    12181 aagcatttca ggatacatat cttttttatc ttctagacca actgtctgca gtaatttgat
    12241 tgcttttttc tctgcttctt ctctagattc tttcttcagg tccatcggtg ctaaaatgat
    12301 gttttccaaa atagaaagat gagggaatag gttaaagtgt tggaatacca ttccgatatg
    12361 ttgtctcacc aagttgatat ctgtgctttt atccatcaag tgggcgccgt caataataat
    12421 ttcaccgtca gaaggttctt ctaaacgatt caagcaacgc aagaatgtac tttttcctga
    12481 acctgaaggg ccaatgatac agacgacatc tccttcgttg atggatacat tgatgtcgtt
    12541 taatactgtg ttatcgccgt attttttgac taaatgctca actaagattt tttctgccat
    12601 cttacttcac cttcttttct aaaacttttg ctaattttgt caatgctgtg atcaaaatca
    12661 agtacatgat cgcaatgatc aagtaaacat acgtactttg tgttgtacgt gcaacaatga
    12721 tcttaccagt ttgcagcaat tcgactacac cgattgcgga aatgatcgtt gtgtctttta
    12781 atgagataac gaactgattg acgaatgatg ggatcatgat gcgtatcgct tgcggcaaaa
    12841 tgattttttg cattgtccga ttataagaaa gacctaaact tctggaagct tccatttgtc
    12901 cgacaggaac ggcgttgatc ccgcctcgaa caatctctgc gatataagca cttgcgttaa
    12961 gagtcaaggt gataacacca gcaaggaagt caggaatcgt aaatccaacg ataccaggca
    13021 acccaaagaa aataaagaac gcaagaacca tcattggaat tcctcggatg atatcgacat
    13081 aaatactagc aattgtgcga agagcgcgta ttggtgctac actgaataat ccaaaaatca
    13141 caccgattac tatagccaaa gcaaaggaga tcaaagttag gagaattgtt ctccaaagtc
    13201 ctttcaacaa tgcttgatag ttgttttgga taagtccaag aaatgttgat tcatcagcag
    13261 tggaagactc actggaagtt tctgaaccat ccgctacata ttctttaata atcttatcgt
    13321 attcgcctgt tctttttaat tctttcaatc cttcattgaa catattaaga agctcaggat
    13381 tttgtccttt tttaacggca aatgcatatt ttccgccaga ttcttttttg atcggtgttt
    13441 ccaattcttg tccttgtttg atcgcataac cgattaccgg ataatcatcc atcattgcat
    13501 cgattgcgcc aacttttaac ccgtcgtaca attgatcagc agcatcaaaa tatttgatag
    13561 aatagccata ttcagattca tggtcgctta aaaattcagc actttcggta ccaacttttg
    13621 ctccgactgt ttttcctttc aaatcttcat aagacttgat cgaagtatct ccttttttga
    13681 cagctaattg gatcccgcta tcaaagtaag aatcagaaaa atcatagctt ttctttcgtt
    13741 catctgttac agtcattccg gcaatcattc cgtctgcttg tcctgattcc aaagcttgca
    13801 aagcagcaga gaaaccacgg tagtccattc ggatgttaaa tccttgcagt ttggcgatgt
    13861 tttccatcaa ttttacatcg atgccttcgt actcgccctt tgcgttttga aattcaaatg
    13921 gagcaaaagc agagtcactg gcaattacgt atacgtcttt tttgggctct attttttgca
    13981 ttgtctcgct tgtgcttgac tctgccgttg tagtgttgtc agcttgtgca acatgaccaa
    14041 acgcaagtgt cacaagtcct gtcattaaga aaacaaatga gtgaaataaa tgctttcttt
    14101 tcataatcaa cctcctaaat ttccaatatt ttattattat aggcgaaaag tgctgaatca
    14161 gacaagactg taagctcgaa aaaatgaaaa aaatgcgagt ttctaacatt ttatgttata
    14221 aaacataaca tgtataataa gataaaaaag tggcgaaagt atgttcggtg tggtataatt
    14281 ttttagtaag gaatgccttt gctttccttt tctttttatt ggtcgtaaaa tgaaaaggaa
    14341 tgaatgaaaa ggaggacgaa gaatgattga gagagatacc tcccgacact ttgagcttgt
    14401 ctcgaaatac caaccggctg gtgatcagcc tgaagcgatt aaccaattag tggatggcgt
    14461 agtcggcggg aaaaaagcac aaatcttatt aggtgcgact ggaacaggga aaacatatac
    14521 gatttccaat cttattgaga aagtgaataa accaacactg attattgccc ataacaaaac
    14581 attggctggt cagctgtacg gggaattcaa ggaatttttc cctaacaatg ctgttgaata
    14641 ttttgtttct tattatgatt attatcagcc tgaagcttat gtgccttcaa gcgatacgta
    14701 tatcgaaaaa gattcaagtg taaatgatga gatcgacaaa ttacgacatt cggctactag
    14761 ttcattatta gaaagaaatg atgtgatcgt cattgcatcc gtttcatgta tttttggatt
    14821 aggctcgcct tttgagtacc aaaaacaagt agtttcgatt cgacagggag cagaactaga
    14881 tcgcaatcag ttgattcgtg acttagtcag tatccaattc gaaagaaatg atatagattt
    14941 tcagcgcggt cgttttcgag tgcgcggtga tgtcgtggag atttttccgg cttcaagaga
    15001 tgaacgtgcc ttacgtgtgg agttttttgg agatgaggtc gaacgtatcc gagaagtcaa
    15061 tgcactgaca ggtgaagttt taggagaaac ggaacacgta gcaatcttcc cggcgactca
    15121 ctttgtgacg aatgatgaac atatggagca tgccgtagcg aatatcaaag cggaattaga
    15181 acagcgttta actgtcttgc gaaatgaaaa caaacttctg gaagctcaac gtttagaaca
    15241 gcgaaccaat tacgatattg aaatgatgtt agaaatgggc tacacgtcgg gaattgaaaa
    15301 ttattctcgg catatggacg gacgtaaaga aggtgaaccg ccttatacgc tattggattt
    15361 ctttcctgaa gattttttga tcgtggcgga cgaatcgcac gtaaccatgc ctcaaatcag
    15421 aggaatgtat aatggtgacc gagcgagaaa acagatgcta gtagattacg gtttccgttt
    15481 gccatctgct ttagacaatc ggccacttcg gttagaagag tttgaaaaac atgtcaatca
    15541 gatcatttat gtctctgcga cgccaggacc atatgaacat gaacaaacag ataccgttat
    15601 ccaacaaatc atccgtccga cagggctgct tgacccagtg atcgaagttc gtccaatcat
    15661 gggacaaatc gatgatctag taggcgaaat aaatgaacga gtcgaaaaag accagcgggt
    15721 gtttgtaacg acattgacga aaaaaatggc agaagacctc acagattatt tcaaagaatt
    15781 agggatcaaa gtcaaatatt tgcatagtga tatcaaaacg ctggaacgaa cagaaatcat
    15841 tcgagatctt cgattaggtg aatttgatgt gttagttgga atcaatttgc ttcgtgaagg
    15901 attagacgta cctgaagttt cccttgttgc tatcttggat gcggataaag aaggcttttt
    15961 acgtagtgaa cgttccttag tccaaacgat tggacgagct gctcggaatg aagaaggtaa
    16021 agtaatcatg tacgcagaca aagtaacaga ttctatgcgt ctagcgatgg atgaaacgtc
    16081 aagacgtcgt acgattcagc agaagtacaa tgaagaacat gggatcgttc caaaaacgat
    16141 cattaaagaa atccgagatt tgatttctat tacgaaagaa tcggaagatg atacaaaaga
    16201 agcggtacaa gtctcgtacg aagaaatgac gaaggaagaa aaagacacgt tattgatgaa
    16261 attagaaaaa gaaatgaaag atgcggcaaa agctttggat tttgaaacag ccgcgaatgt
    16321 gcgagatatg attttagaat taaaagcttc aaagtaggag gacaacatgg ctaacgataa
    16381 aattacgatt catggtgcac gtgcccataa cttgaaaaat atagatgtaa cgattccacg
    16441 agataaattc gtggtcgtta caggtttgtc cggttctgga aaaagttccc tagcatttga
    16501 tacgctgtac gctgaagggc agcggagata cgtagagagc ctttctgctt atgctcgtca
    16561 gtttttagga caaatggata agccggacgt agatagcatc gacggattga gtccggcgat
    16621 ttctattgat cagaaaacaa caagtaaaaa tcctcggtcg acagtaggta ctgtgacgga
    16681 aatcaatgac tatttacgct tgttgtttgc acgtgtagga catccgattt gtcccaacga
    16741 tcatatcgag attaccagtc aatcagtaga tcaaatggca gatcaagtaa tggctttgcc
    16801 tgagcgcaca aagatccaag tgttggcacc gatcgtggta aagaaaaaag gacaacataa
    16861 aaaaatattc gaacgaatcc aaaaagaggg atacgtccgc gttcgagtcg atggtgagat
    16921 ctatgattta agtgaagtgc ctgagctgga gaaaaacaaa aaacatgata tcgcgatcgt
    16981 catcgaccgg atcattgtga aagaagggat tcgttctcgc ctatttgatt ctttagaggc
    17041 ggccctgcga ttagcagacg gctatgctct ggttgacgtg atcggtgaag aagaaatgtt
    17101 gtttagcgaa cactatgcct gtccttattg cggatttact gttggcgaac tagaaccaag
    17161 actgttctca ttcaatgcgc cttttggagc ttgtccagac tgtgatggtt taggtgtcaa
    17221 attagaagtc gatcaagatt tagtgattcc agatagaacg aagacactta gtcaaggtgc
    17281 tatcgttcca tggaatccaa tcagctctca gtattatcct caaatgttag cgcaggcttg
    17341 tgaaagtttc ggtatcgatt tagatacacc attcgaagaa ttaccggaag agcatcagga
    17401 gatcatcttg aatgggacag atgatctttt ccatttccat tatgaaaatg attttggtgg
    17461 cgttcgcgat gtagaaacga ctttcgaagg ggtattgaag aatatcgagc gtcgttatca
    17521 tgatacgaac agtgatttca cgcgtgagca aatgcgtctt tatatgacag aattaacttg
    17581 tcagacatgt aaaggttatc gattgaatcc ccaagctcta tctgttcaaa tcaatggaac
    17641 gaatatcggg caaacaaatg agttgtctat tcaaaaagca gtagatttct tctcaaactt
    17701 aacgttatct gaacaagaga aagtaattgc caaaccgatt ttaaaagaag taaatgacag
    17761 actttcattc ttggaaaatg tgggactaga ctatctaact ttgagtcgcg cttccggaac
    17821 attatctggg ggagaagcgc agcgaatcag attggctaca caaattggat cgaatctttc
    17881 tggagttttg tatattttag atgaaccatc gatcggattg catcaacgcg acaacgatcg
    17941 actgattggc tcattaaaga aaatgcgtga tcttggcaac accctcgttg tagtcgaaca
    18001 tgatgaagat acgatgtggg cagcagatta tttgattgac gtcggacctg gggccggaga
    18061 acaaggcgga caaatcgtgg ctgcaggcac tcctgaagaa gtagctaatg atgaaaattc
    18121 tttgacaggg aaatatcttt cagggaaaaa atcaatccct gtaccaaaag aacgccgtaa
    18181 aggtaatggg aaagcaatca agatcactgg tgcttcagaa aacaatctga aaaatattag
    18241 tgttgaattt ccattaggag aatttgtagc agtcacaggt gtgtctggtt ctggaaagag
    18301 tacattggtc aatagtatct taaagaaatc gttagcgcaa aaattaaata agaattctgc
    18361 taagccaggt aaattcaaga caatttccgg ctacgaaagt atcgaaaaga tcatcgatat
    18421 cgatcaaagc ccaatcggcc ggacgccgag aagtaatcca gcgacttata caagtgtatt
    18481 tgatgatatc cgtgggttat ttgctcaaac gaacgaggca aaaatgcggg gttataagaa
    18541 agggcgtttt agtttcaacg taaaaggcgg tcgttgtgaa gcttgtcgcg gggatggaat
    18601 tattaagatc gaaatgcact ttttgcctga tgtctatgtt ccttgtgaag tatgtcatgg
    18661 taaacgatat aattctgaaa cattagaagt gcattacaaa ggaaaaagca ttgctgatat
    18721 tttggaaatg acagtagagg atgctgtaga attcttcaag cacattccaa agattcatcg
    18781 caaactgcaa acgattgttg atgttggctt aggttatgtg acgatggggc aaccagcaac
    18841 gacattgtcc ggtggtgagg cacaacggat gaaacttgcc agtgaattgc acaaaatctc
    18901 taatggaaag aatttctata tactagatga accaacgaca ggacttcata gcgatgacat
    18961 cgcccgcttg ttgcatgtat tacaaagatt agtagatgct ggtaacacag ttttagtgat
    19021 tgaacacaat ctagatgtaa tcaaaacagc agattatatc attgatttag gaccagaagg
    19081 tggagaaggt ggaggaacga tccttgcgac tggaacacca gaagaaatca ttaacgtaaa
    19141 agaaagttat acaggtcact atttgaaaaa aataatggta taaagaggat agatagctga
    19201 gatagttaaa aaaacataaa aaaatgaatt tttgcttgtt ttattaatat cagtgttcta
    19261 gaatcattac atggtagttc tttctacact gattggtata gataaaaact tcctataagt
    19321 tcaatccgtt caaaaaagaa ttgagcaatc ggaggacttt cctttgattg cttttttctt
    19381 tatggtatga tattccttgt gtagttggaa aagaaaaaca agagattatg agtaagtgag
    19441 agataatgat gaacagtatt gaaaatgaag aatttatccc tatattatta ggaagcgaca
    19501 tgaacgttta tggtatggca cgttctttta atgaagcata cggcaaaatc tgccaagcat
    19561 atgcttcgga tcagttagcc cctacccgct acagtaaaat tgtcaatgta gaagttattc
    19621 caggatttga taaagaccct gtatttatcg agacgatgct tcgtttggca aaagaaagat
    19681 attctgataa gagtaaaaaa tatcttttga ttgcttgtgg tgatggatat gcagaattga
    19741 tctcccaaca taaacaagag ctgtcagaat attttatctg tccatatatt gattattctt
    19801 tatttgaaag gctgatcaat aaagtaagct tttatgaagt ttgtgaagaa tatgatcttc
    19861 cgtatcctaa aacattgatc gtcagagaag aaatgttagt aaatggacat ttggaacagg
    19921 aattgccttt tgaattccca gttgctttga aacctgcaaa cagtgtggag tatctttctg
    19981 tccaatttga aggaagaaaa aaagctttta ttttagagac tagagaagag ttcgatttaa
    20041 ttttaggccg gatatacgaa gccggttata aaagtgaaat gattgtacaa gattttatcc
    20101 cgggtgatga tagtaatatg cgtgtgctaa atgcgtatgt agatgaagat catcaagttc
    20161 ggatgatgtg ccttggtcat ccactattag aagatcctac accagcatcg attggtaatt
    20221 acgttgtgat catgccagat tataacgaaa aaatctatca aaccattaag gcatttttgg
    20281 aaaaaatcga atatacaggc tttgccaatt tcgatatgaa gtatgatcct agagatggag
    20341 aatataaatt atttgaaatc aatttgagac aaggtcgtag cagtttcttt gtgacattga
    20401 acggcttgaa tctggctcgt tttgtcacag aagatcgagt gttcaacaaa ccctttgttg
    20461 aaacgaccta tggaacgaat caatcagata aagcaagatt atggatgggt gtgcccaaaa
    20521 aaatcttttt agaatatgct cgggaaaatg aagacaaaaa actagctgag caaatgatca
    20581 aagaaaatcg ttacggtact actgtatttt atgaaaaaga ccgatcaatc aagcgctggt
    20641 tgttgatgaa atatatgttc cataactata ttccaagatt caaaaagtat tttcatgtga
    20701 aagaaggcta gttcatggag aattttttca gtattttagg cggaatgggc acgatggcga
    20761 cagaaagttt tgttcgcttg atcaaccacc gaacgaaagc tacaaaagat caagaatatt
    20821 tgaattatgt cttattcaat catgcaacag ttcctgatcg tacagcttat attttagatc
    20881 gatcagaaga aaatccaatg ccgtttctgc tggatgatat tgagaaacaa aatttattgc
    20941 gaccaaactt cattgtgttg acttgtaaca ccgcacacta cttttttgaa gagttacaag
    21001 cggcaacgga tattcctatc ttgcatatgc caagagaagc agcaaatgaa cttgtccgtc
    21061 agcatacgac agggagagta gcgattctag gaacagaagg aagtatgaaa gctggaatct
    21121 atgaacgcga agtcaaaaat cttggtttcg aaacagtgat tcctgatact gcgcttcaag
    21181 aaaaaatcaa ttacctgatc tatcatgaga tcaaggaatc ggattatttg aatcaagagc
    21241 tctattacga aatcttagag gaagcggtag agcgcctaaa ctgtgaaaaa gtcatcctag
    21301 gttgcacaga attatctttg atgcatgaat ttgctgaaga taatcattac ccagtgatcg
    21361 atgctcaatc tattttagcg gatcgaacga tcgaacgtgc attagctgaa agaagcgagg
    21421 cattggatac agcatcggaa aagtaaaaaa attggatttg gggcaaatat cctaaaagat
    21481 atttggtcca aatctttttg taaaagaaaa agaaaactgg taatgatacg cccgggatga
    21541 tataatttta ttatgcacaa tgtgagaggg gttcgtaaat atgacagata atttgcaatt
    21601 agtcatcata acaggtatga gtggtgcagg gaaaacagta gctatccaaa gttttgaaga
    21661 tatgggctat ttttgtatcg ataatatgcc gcctcgattg attccaaaat tctgggaatt
    21721 gatcaaagaa tcaggtaaag tcacaaaaat ggctttagga gtggatttgc gttctcgttc
    21781 cttctttgaa gaaatccaaa acatgctgat cgaaatcgaa aatacgaact tcatcgacac
    21841 ttctatctta tttttagatg cttcagacga agaactagtg gctcgctaca aagaaacaag
    21901 acgagcacat ccaatggcga tggacggttt gatcactgaa gggatacgta aagaacgggc
    21961 gatactagac gacttgaaag cccaagcttc cgtgatcatt gatacgacaa cattgacacc
    22021 acgacaatta cgtgaaaaaa tcaatgagtc attcaaatca aagggagacc aaggatttcg
    22081 gatcgaattt gtatcatttg gttttaaata cgggttgccg atcgattcgg atattgtgat
    22141 ggatgtgcgt tttttgccca accctcatta tatcccggaa ctaagaccat tgaccggaaa
    22201 agatcagcca gtctatgatt atgtgatgtc ctttgatgaa acgcaatcat tctaccataa
    22261 atttttggat cttctggaaa ctgtattgcc tggatacatt aaagaaggaa aaagcagctt
    22321 gacagttgca atcggctgta caggcggtca acaccgttct gtggcattga ctgaacgttt
    22381 ggcaaatgct ttagggaaaa actataaagt caatgtgaca catcgggaca aggataaaag
    22441 gaaagagacg gtgaaccggt cgtgaaaatg aaaacctacc ggataagaaa accaaagatc
    22501 gtagtggtcg gcggtggcac tggtctgcca gttatcctga aaagtttacg aaatcaagga
    22561 gcagatatca cagcagtagt gactgttgca gatgatggtg gaagcagcgg tgcaatcaga
    22621 gaatcgatcg ctatggctcc tcctggtgac ttgagaaatg tcttagtggc gttatcggat
    22681 atgccacaat tctatgaaga tattttccaa taccgtttca aaaaggaaga tcagttttta
    22741 gccaatcata cgatcggtaa tctgatcatt gccgcagttt ccgagatgcg gggaagcact
    22801 tatgaggcga ttcaattatt agcaaaaatg atgcatgtag atggacatgt ttatccttct
    22861 tcagaaactc ctttgacctt gcatgctgtt tttaaagacg gaacgactgc agtaggagaa
    22921 tctaaaatcg cgatcaaccg taaaaccatt gaccgtgtgt atgtctgcaa cacaaatgat
    22981 aaaagtgaag ccaaagcggc tcgaaaagtc gtttcagcaa ttatggacgc cgacatggtc
    23041 gttttgggac ccggaagttt attcaccagt atccttccaa atttagtgat accagaaatc
    23101 ggcgaagcaa tgctcaagac aactgctgaa accgtctata tctgtaatat catgacccaa
    23161 aaaggggaaa cggaacattt cacagatgcc gatcatatac gtgttttaca caagcatttg
    23221 aatcagcctt ttatcgatac agtattggtc aatacggaaa aagtgcctga agattatatg
    23281 gacccagaga tctatgatga gtatctagta caagtcgcac acgacttcaa cggattgaga
    23341 aatgaaggat gtcgagtgat ttccaccgac tttttagaat tgaaagatgg tggtgtcttc
    23401 cacgatggag acaaagtagt agaagaattg ttccgtttag ttttcggttc aaaatattaa
    23461 gcaatttatt gttggagaaa ggaggagaat aggatgtcat ttgctgcaga tgtgaagaaa
    23521 gaactaactg gtttggaagt tcatcgagaa catgcgaaag cggaattagc tgctttgctg
    23581 cgaatgaacg gctctttagg tattgtgaac cagcagttta ttttgaacgt ccaaacagaa
    23641 aatgctgcaa ttgccagacg tatctattct cttttgaaag atcattatca ggtacgaagt
    23701 gagctactcg ttcgtaaaaa aatgaaatta aaaaagaaca atgtctatat cgtccggttg
    23761 aagcaaggca caaaagatgt gttgatggat cttgatatca tggatggtat gatgttcaat
    23821 ggacacgttt cggacgagat tatgggaaat tcgcaaaaaa tgcgttctta cttacgtggt
    23881 gcttttatgg cctcaggttc agtcaataat ccggaaacga gtcgttatca tttggaaata
    23941 ttttctattt atgaacagca taaccaagac atttgcgata tgttaaatta ttacggtcta
    24001 catgctcgaa cgcttgaacg gcgcaatggc tatatttcgt atttaaaagg agcagaaaaa
    24061 attgctgatt tcttaacatt gattggtgca accaactcaa tgctccgatt tgaagatgtt
    24121 cgaatcgtac gggatatgag aaattcggtc aatcgtctag tcaattgtga gacagcaaat
    24181 ctgaacaaga cgattgatgc ggcttctaaa caaatcgaaa acatcgaatt gatcgaagca
    24241 aaggtcggtc tacatgccct tcctgaaaaa cttcaggaaa tcgcagaact tcggcttcaa
    24301 catccagagg tcagcttgaa agaattaggg gaaatgattc cctcaggtgc aatatcaaaa
    24361 tcgggaatta atcaccgaat tcgcaaaata aatgaatttg cggaaaaact gcgtgaagaa
    24421 gcagtttaat ccaaagaaaa aactgataca tgaagcagga gatgcgaatg tatcagtttt
    24481 ttttaaaagc tatacttgct tttcttccgt caaactataa ttccaacttt actggagtaa
    24541 cataatgttg aaaggcttct attgtttttt aatacaatcg tataaaaagc ggggagacaa
    24601 ctgaatgaag attttagttt tatacataaa cttttgctta atatagaagt cctgatatag
    24661 ataggatcaa gtatttatct ataactggag attaattaaa aactggaagg aactgctcga
    24721 tcaaccttga gaactgtttt tcttacttct ggtcaagatt ctcgtggttg gtggataagg
    24781 atggttgata agttcattgg attagctagt cgaaaacaac caggtgtaat taatgtagaa
    24841 gttttaacaa ggggatggag tgatttattc agtgataatc ataagttcat aaatagtaat
    24901 tgggatgtat ggttggtata cgttatacct gaacttgaac tatcaagatt tggtttagaa
    24961 tttgagaatc gatgtttata aagggagggc aaaaaatgtg cttttttaca acttttacta
    25021 tcattatgat tgcagtactt tttatttttc tagatatagc aaagcgaaat acagcatttt
    25081 tattatatcg ggttttactt cgagcaggtc tcataacatt tatatcgatc gttggatttt
    25141 ttctattcac agttattgta tttatttgga gaactacagc accaccattg ccagagatta
    25201 catatggaga atttccgttt agtcttgaat atgaaataga tgaggaaaga attttgattg
    25261 aagatacggt gattgcagaa tttataggaa gttttagagg gaatgtgaca actcaaccta
    25321 gacgaagttg gagtataaaa cttttaaata atagtggaga gttgaaagca tttcattatg
    25381 aattcttaat aatagaaagt gaagatgtaa caatcacttt aagtagtggc tctgctggat
    25441 attttatgaa tgatggtgga agcaagccac agctcacagt ccgtgatcgt attgcaggtt
    25501 ttatgcattt ttctcactat gacttagaag aagctcgtaa aatacttgca gaatatggga
    25561 tcacattaat caattgggaa tatgcgccac cgataagaaa ttttttcaat gattattatt
    25621 agtcttataa aaaacagaat gtaaaattta tgtgcgcgag tatttagttt tccatttttt
    25681 gtttgataga tatatagcat tgttgaagca tgggatagtg gtgccaattt tatcaatgag
    25741 gttggtcagg cagttaacat aattggtgat gctataaatc catttaattg gaattggagg
    25801 aattaggtgt ggaaacagtg agattatgga tattcatgaa aaattttcgt tcggctttga
    25861 gatatgttat tctggaatca aaatcaggtt attatctgat tgacacagat cactatactt
    25921 tttggggagg ctaccttttt ccgattttta attggataat cccccataag gttatgaaaa
    25981 ttaacttgcc tgcagaagaa ataaatagat taatactcaa caaagattta caaaggaaaa
    26041 gagagaaaga acattctaat gttgcttgga taggtccagc atttgcagta gtgacttctt
    26101 caattgtcgg tcctatgcta gagagtattt atagtgattt ttctattgga atacgttatt
    26161 tatttgttac tttaagtata gctgttcttt tgtgcataaa gggttatgtt agcaagaaga
    26221 ataaagatat tttaacacta atcggtgtaa acaatttatc tagtactaaa attatactgt
    26281 ttcctgaatc tataaaagaa ggatcaaggt tgttgataat ttttataatg cttatactgt
    26341 tttctgtttt atttggggtg atgtttatag
//
LOCUS       DAZKNH010000031        25993 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000031 DAZKNH010000000
VERSION     DAZKNH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.031"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..448
                     /locus_tag="ACG8MG_001225"
     CDS             131..448
                     /locus_tag="ACG8MG_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0375281.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(652..1017)
                     /locus_tag="ACG8MG_001226"
     CDS             complement(652..1017)
                     /locus_tag="ACG8MG_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375282.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1225..1707
                     /locus_tag="ACG8MG_001227"
     CDS             1225..1707
                     /locus_tag="ACG8MG_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375283.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1904..2794
                     /locus_tag="ACG8MG_001228"
     CDS             1904..2794
                     /locus_tag="ACG8MG_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFQ0375284.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3119..5590
                     /locus_tag="ACG8MG_001229"
     CDS             3119..5590
                     /locus_tag="ACG8MG_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0375285.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5693..7429)
                     /locus_tag="ACG8MG_001230"
     CDS             complement(5693..7429)
                     /locus_tag="ACG8MG_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0375286.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7426..8130)
                     /locus_tag="ACG8MG_001231"
     CDS             complement(7426..8130)
                     /locus_tag="ACG8MG_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0375287.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8261..8764
                     /locus_tag="ACG8MG_001232"
     CDS             8261..8764
                     /locus_tag="ACG8MG_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFQ0375288.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8724..9062
                     /locus_tag="ACG8MG_001233"
     CDS             8724..9062
                     /locus_tag="ACG8MG_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFQ0375289.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9083..10501
                     /gene="ffh"
                     /locus_tag="ACG8MG_001234"
     CDS             9083..10501
                     /gene="ffh"
                     /locus_tag="ACG8MG_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFQ0375290.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10613..11191
                     /locus_tag="ACG8MG_001235"
     CDS             10613..11191
                     /locus_tag="ACG8MG_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFQ0375291.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11195..11716
                     /locus_tag="ACG8MG_001236"
     CDS             11195..11716
                     /locus_tag="ACG8MG_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFQ0375292.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11795..12349)
                     /locus_tag="ACG8MG_001237"
     CDS             complement(11795..12349)
                     /locus_tag="ACG8MG_001237"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0375293.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12602..12877
                     /gene="rpsP"
                     /locus_tag="ACG8MG_001238"
     CDS             12602..12877
                     /gene="rpsP"
                     /locus_tag="ACG8MG_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFQ0375294.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12889..13140
                     /locus_tag="ACG8MG_001239"
     CDS             12889..13140
                     /locus_tag="ACG8MG_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFQ0375295.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13265..14056
                     /locus_tag="ACG8MG_001240"
     CDS             13265..14056
                     /locus_tag="ACG8MG_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375296.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14226..14747
                     /gene="rimM"
                     /locus_tag="ACG8MG_001241"
     CDS             14226..14747
                     /gene="rimM"
                     /locus_tag="ACG8MG_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFQ0375297.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14737..15498
                     /gene="trmD"
                     /locus_tag="ACG8MG_001242"
     CDS             14737..15498
                     /gene="trmD"
                     /locus_tag="ACG8MG_001242"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFQ0375298.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15634..15981
                     /gene="rplS"
                     /locus_tag="ACG8MG_001243"
     CDS             15634..15981
                     /gene="rplS"
                     /locus_tag="ACG8MG_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFQ0375299.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16354..17016
                     /locus_tag="ACG8MG_001244"
     CDS             16354..17016
                     /locus_tag="ACG8MG_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375300.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            17095..20322
                     /gene="fss3"
                     /locus_tag="ACG8MG_001245"
     CDS             17095..20322
                     /gene="fss3"
                     /locus_tag="ACG8MG_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0375301.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            20436..20750
                     /locus_tag="ACG8MG_001246"
     CDS             20436..20750
                     /locus_tag="ACG8MG_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0375302.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            20763..21137
                     /locus_tag="ACG8MG_001247"
     CDS             20763..21137
                     /locus_tag="ACG8MG_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0375303.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            21147..21491
                     /locus_tag="ACG8MG_001248"
     CDS             21147..21491
                     /locus_tag="ACG8MG_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0375304.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            21562..22914
                     /locus_tag="ACG8MG_001249"
     CDS             21562..22914
                     /locus_tag="ACG8MG_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0375305.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            22974..24116
                     /locus_tag="ACG8MG_001250"
     CDS             22974..24116
                     /locus_tag="ACG8MG_001250"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFQ0375306.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            24141..24395
                     /locus_tag="ACG8MG_001251"
     CDS             24141..24395
                     /locus_tag="ACG8MG_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375307.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            24651..25835
                     /gene="mobT"
                     /locus_tag="ACG8MG_001252"
     CDS             24651..25835
                     /gene="mobT"
                     /locus_tag="ACG8MG_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0375308.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            25832..25969
                     /locus_tag="ACG8MG_001253"
     CDS             25832..25969
                     /locus_tag="ACG8MG_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0375309.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         8695 a         4546 c         5525 g         7227 t
ORIGIN      
        1 taactatgca ctgtaacgat aatgctgcta aaatatcttt ttacgaaaaa catcacaagg
       61 aaaaacttaa ctatggtatt atctaaacat aattttttcg gtatttaata tttaattagg
      121 agggaatatt ttgctttttt atagtaattt tatcttaata gtagcgatat tattacttct
      181 gaatatatgg atttttgata ggagtagaaa tgcaagtata ggttttagaa ctaaacgaag
      241 tctgtcttct aaaaaaaatt gggtatactc acaaactatt ttttatggag gaattgtttt
      301 aattagttta ctttcttcaa cactttattc tttgaatatt atagatgttt ccacctcaaa
      361 ttcaatatct attattggaa tcattattgc tgcaattatt acacaactgt ttttagtgtt
      421 tggagagaag aaaagaagta aaaaatagaa aaactagtga tagttttggt gatagtgata
      481 ttaaaaatca gaaaagatag aaaaagcagg agatagataa aatgctgata tttcagcatt
      541 ttttaagagt ataaaagaga aaaaaagtaa taaatgtata catgatataa gaaaaacata
      601 tatgtataaa aagaagaaag tagcttctct ttgtacatat atggtttgtt tttatggatg
      661 ccgagctttt ttataagata aggctaccgc ccataacgac ccaaggattt gtggaactaa
      721 gtaaatacct gcaagaagca agatcagctg ggtcggtttt aaaggatgat agaagtaacg
      781 ccattcagta aaattggcgc aaaataaata ggaagtgaca gtagatataa tattggcaat
      841 gaataaagat ataatacgcc tgttcctttg aaaataatat ccagcaaaaa gagataaaaa
      901 tatcaatgtg atgaacccgc ttacggaatg ataacgaaaa tcaagcagta aggcgaaatg
      961 cacataagga acaaaagata aaagcaacgg aaggtttttc cagcaagttt ttatcatctt
     1021 tcatcaccac ctaacaagtg ttattatttt gttagtttta ttgtaaattg aatcctgcaa
     1081 taatcaatta ctttttcatt atttctagat taaatgaata aactctcatg ccattatctc
     1141 taaaaaaaaa gtttttcccc ctaatttttt tagatagaaa attgtatagt atgatgagag
     1201 aaaaagtagt ttgggggaac gttaatgaga agaaaagcag taatcgatcg gctttattta
     1261 tttttttggt tttttcctat cgttttgcta ccttttacat gggtaaatgc tgttgtacaa
     1321 ggtgcggaga tattaggcct gatccaagga agcggatttg tggtttctag gaattttcca
     1381 gctttgactg ttttctattt agtgataagt gtaggtctct tattaccaaa atctctgatc
     1441 aatcactaca attggcagct gctttacttt tcttgcttat tagtatttac atctatgttt
     1501 tctttgctgc ctcgcttgat tatcggccct aatggtttga gcgagagtgg attgaaggat
     1561 tcagcttatt cctccatgta tttcttgacg atcaaaccat ggttctattt agtaattgcc
     1621 tcatttattc ttgcaacatt tttttacttc tggacagaaa tcaggataaa aagtgaacga
     1681 tctgtatcta taaaaaataa ctattgatgg tggatcatca gtaaaaaagc atatgagctg
     1741 cgtaaaatgc atgtttctca tatgcttttt ttattattat gtaaagaatg cgaaaaacgt
     1801 ttacatgatt tttacataac ttaactatat ttttacagca accatacgag ggatttacag
     1861 tcgactgata aactttagtt gtaaataaga aaggagcaat aaaatgaaaa aagtattgtt
     1921 tactctaggt atgatgggaa ttcttttagc cggatgcggc tcaggatcag gaaatacaga
     1981 tggctctgcc acacaaaata attcaagtga ctcaaatgaa caagtaaaaa tcgttgcggt
     2041 aggttcgaca gcattacagc cgttagtcga tgcagcacaa gaaagctttg tacaagaaaa
     2101 tccaaattat caaatctctg tacaaggagg aggaagcgga actggtttga gccaagtcga
     2161 agctggagca gttactatcg gaaactctga tgtatttgca gaagaaagag acggagttga
     2221 tgcttcaaaa ttagtggatc ataaagtagc agtggtcggg atggcgccaa tcgtcaataa
     2281 agatacagat gtcaaagata tcaccaaaca ggaattgatt gatatcttta caggaaaaat
     2341 cactaactgg aaggaagttg gcgggaaaga tcaaaaaatc aacgtcgtga accgtgcaaa
     2401 cggcagcggt acccgcgcga cattcgaaaa atggggactg gatggtgcta cccctgtgca
     2461 gtcccaagaa caagattcat caggaacagt tcggcagctg gtaagtcaaa caccaggagc
     2521 aatcagctat ttagctttct catatctcga tgattccaca caagcactaa gtatcgacgg
     2581 tgtagaacca aaagaagaga atgtagcaga taacagctgg ggaatctggt catatgaaca
     2641 tatgtacacg aacggaaaac cttcccctga agtccaaaaa ttcttagact acatgatgac
     2701 agaagaaatc caagaaggac ctgtcaaaga attgggctat ttaccaatca caatgatgga
     2761 agtagaacgt gatcacgaag gaaatatcaa ataactagca gataaacatg ccacggtagt
     2821 cgttactatt aaggaaaaat caactatgtt tcccacatat acatttttcc tttaattgtg
     2881 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattaaa
     2941 caccgtttat tcgaaaaaaa gagctacgac atagaactgt cgtagctctt tttttgctat
     3001 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
     3061 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
     3121 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
     3181 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
     3241 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
     3301 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     3361 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     3421 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     3481 tacagcaaaa gtcaatgtca tacgggacgg gatagaacaa acaatagata tagaagacct
     3541 tgtaatagat gacattgtta ttttagagac tggacatcaa attccagcag atgcagaggt
     3601 cgtggaagga aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     3661 caaagaaata ggcgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     3721 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     3781 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     3841 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     3901 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     3961 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     4021 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     4081 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaag tgatcgtctc
     4141 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     4201 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     4261 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     4321 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     4381 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     4441 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     4501 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     4561 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     4621 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     4681 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     4741 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     4801 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     4861 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     4921 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     4981 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     5041 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     5101 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     5161 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     5221 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     5281 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     5341 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     5401 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     5461 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     5521 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     5581 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     5641 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     5701 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     5761 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     5821 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     5881 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     5941 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     6001 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     6061 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     6121 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     6181 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     6241 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     6301 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     6361 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     6421 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     6481 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     6541 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     6601 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     6661 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     6721 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     6781 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     6841 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     6901 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     6961 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     7021 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     7081 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     7141 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     7201 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     7261 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     7321 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     7381 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     7441 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     7501 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     7561 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     7621 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     7681 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     7741 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     7801 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     7861 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     7921 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     7981 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     8041 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     8101 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     8161 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     8221 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     8281 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     8341 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     8401 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     8461 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     8521 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     8581 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     8641 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     8701 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     8761 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     8821 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     8881 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     8941 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     9001 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     9061 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     9121 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     9181 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     9241 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     9301 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     9361 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     9421 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     9481 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     9541 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     9601 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     9661 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     9721 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     9781 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     9841 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     9901 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     9961 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
    10021 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
    10081 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
    10141 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
    10201 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
    10261 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
    10321 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
    10381 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
    10441 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
    10501 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
    10561 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
    10621 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
    10681 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
    10741 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
    10801 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
    10861 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
    10921 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
    10981 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
    11041 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
    11101 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
    11161 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
    11221 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
    11281 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
    11341 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
    11401 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
    11461 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
    11521 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
    11581 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
    11641 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
    11701 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
    11761 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
    11821 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
    11881 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
    11941 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
    12001 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
    12061 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
    12121 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
    12181 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
    12241 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
    12301 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    12361 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    12421 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    12481 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    12541 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    12601 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    12661 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    12721 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    12781 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    12841 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    12901 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    12961 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    13021 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    13081 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    13141 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    13201 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    13261 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    13321 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    13381 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    13441 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    13501 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    13561 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    13621 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    13681 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    13741 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    13801 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    13861 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    13921 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    13981 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    14041 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    14101 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    14161 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    14221 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    14281 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    14341 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    14401 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    14461 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    14521 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    14581 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    14641 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    14701 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    14761 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    14821 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    14881 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    14941 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    15001 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    15061 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    15121 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    15181 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    15241 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    15301 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    15361 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    15421 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    15481 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    15541 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    15601 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    15661 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    15721 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    15781 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    15841 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    15901 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    15961 atcaaagaaa tcagaagata aacacggaaa acttccttgg aaatctgaaa tttgtgaaaa
    16021 gtccttgtat atcaagggct ttttcttttt tctctatcac agttttaagc catttttggc
    16081 tgttttggac catttttcaa tcatattccc ttaaaaattg tcctagattg tccgacattg
    16141 actatcagtg tcgtcaaaat agggcaaaat ttagtagtca gcttgacgac aaatataata
    16201 cgaaaaatac caaaacattt accgaattta gcaacttttg aacccaagtt tctcaaaggg
    16261 tcagctatta gcaaaaaaga caaaacatgt acttttctac taaaaagttg atgtgatagg
    16321 cttctatcta aataactatt aggagttttt atcgtgaagc aaaatgatgg acgtatcatt
    16381 tggaaaaatc aaagtgagtt aaaactgatt ttaacaatta atgaattcat cgaaaaacac
    16441 ggaattacat ctagccgtca ataccagaaa aagctatcag aaaatccaaa ttctgcacca
    16501 agtatgtggt tcattaacaa aaaatatgga tcatgggaga atttattgat tagtatagga
    16561 cgagaaaata ctgggtatgg aaaatgggct agaatgtcag aacaagaatt attggaaata
    16621 gtagaagcct ttattaagtg cgagaagata acctctcaac gaatgtacga acaaaaatca
    16681 gtagggaaga atattccttc acttagtacc attaaaaaga tgttaggaga tatacgtcca
    16741 ctttttaaag aaaaaaatga tggatctcga tttactgatt tcgaactgct gttagaacta
    16801 aaaaatgaaa taatccgatt aaaactacag gatgatttat caatgacaaa gtttcgaaaa
    16861 ctagttcaat ccccaaaact accatcagtt gatacaatta tgaaaagaac gaacaaaaat
    16921 tgggaggaat tgatggcaga gattggattt gattatcgaa gaatcaaaat ttataagcag
    16981 agaaacaacc tatccaagac aaagaaaact aaatagtatc actctaaatc agacgttcaa
    17041 aattaaaatt tggacgtctt ttttattacc caaaaacgaa aggagattta ttttatgaaa
    17101 aagccaagat ttaagaattg gcgtttgctc gcaacactga gtctattatg ccaaacaatt
    17161 ggtggatcgc ttggcccaac gattgccttt gctgatgaaa ttactcatcc acaaacggta
    17221 actgtggaat tggatttggc tcaccagtat gctgtcgaag gtacatttag cgatggtcgt
    17281 cccatgtcag aagttaccgt tccgcactat gcagtttaca atggtgtgaa gcaagatatt
    17341 ttctgtattg agccaggggt gccgatttac aatgagttca ctcctggtta tgagaaaaat
    17401 ccattgcctg atatgtcgga gaaagcgaaa ttagtttccg ttttatggaa ggaagctggt
    17461 acggatgtgg atacacatat tgtggcccag aagatgattt ggcaagaagt taatggctac
    17521 acgcttcact caattaaacg ttctgatggt agtgcagtaa atatcgcagc cattgagtca
    17581 aaaatcaatc aagccattgc ggattatcaa aagaaaccaa gcttccataa ctccacagtg
    17641 aaaaccgtgt taggtcaatc gactactgtg acagatacga acggcttgaa tttatcggag
    17701 tttgatgaag ttgtcgagaa tatcgcaaat attgattatc gtgtgaacgg caatcaacta
    17761 gtgattactc caaatgccaa ttcaaaagag agtggtgaat taaccctaaa gaagtcagct
    17821 aatacaggaa caccggtagc ttataaaatg gctggtcaac aaactttgat ggcgggggca
    17881 attgataagc ccaataccta cacaatcaaa attgatgtgg aaactgaggg ttctttgaag
    17941 attaaaaaag tcgataaaga aacaggaaat gttgtaccgg gaaccgtttt ccatttagac
    18001 tttggaaaaa cattacctgc aaaagacgtg accaccgata aagaaggcat tgcgacctta
    18061 gatgagattc ctcatggcgc gaaagtaacc attactgaaa aatcagtgcc agctccttat
    18121 acgattgaca ctacaccaat caccgcaacc attaaatctg gtgagactat ttctgtgact
    18181 tcaaaaaata ctcgcgaaaa aggtcaaatc attttgaata aaatgggggt ggaaacagga
    18241 actgatcttt ggaacgacaa atactcatta gccggaaata cgtttgccat tcgcaaagac
    18301 agtccaaccg gtgaaattgt ccaagaaatg accacggatg aaaaaggtca tgcggaaaca
    18361 ccaaaagaga ttgccaatgc gttggaattg ggaacctatt atgtgaccga aaccaaagct
    18421 agtaatggct ttgtgaatac ctttaaacca gtaaaagtcg aattgaacta tgtcaatcaa
    18481 accgtggctc ttgttaccag taacgtaaaa gggcaaaacc aagaaatcac tggtgaaacc
    18541 actttgacaa aagaagacaa agataccggt aatgaaactc aaggtaaagc tgagtttaaa
    18601 ggagctgaat atactctctt tactgcaaaa gatggtcaag cggtcaaatg gagtgaggct
    18661 tttaaaccag agttagtgaa gggaacgaaa gcttctgatg aaacggtgac tttgacttta
    18721 gatgaaaaga atcaagttgc tgttaaacac ttagcgatta atgagtatta ctggcaagaa
    18781 accaaagcac ctgaaggata taccttggat gaaacaaagt accctgtttc catcaaaaag
    18841 gtggatgata acgaaaaaaa tgccgtgatt acccgagatg ttacggcaaa agaacaagtc
    18901 attcgctttg gctttgattt ctttaaattt gctggatcag ctgctgggac tgccgaaact
    18961 ggcttcaatg atctgacctt taaagtatcg ccattggaag gaaccagtga gattacaggt
    19021 gccgaagatg aagcaattac agcttataac gagcaactag gttttgatgg ctatggcaag
    19081 tttgaaaatc ttccttatgg ggattattta cttgaagaag tagaggctcc agaaggattt
    19141 caaaaaatta cgccattaga aatccgttct acttttaaag aaaataaagc ggattacact
    19201 aagagtgaat acatttttac gattaccgaa aaaggacaaa aacaaccaat taaaacggtg
    19261 accgttcctt acgagaaact gacgaataaa gcattttctg ttagcttgaa tcgtttgatg
    19321 ctctatgatt tacctgaaga agaagatagc ttaacttctc ttgggacttg gaaagacgga
    19381 ggcaaagaat tgacagacct tgattctacc gagctagttg ataaattgag ctataacttg
    19441 catgaaatca aagaagactg gtatgtggta gcgcaagcca ttgatgtgga agcgacaaaa
    19501 gctgcccaag aaaaagatga aaaagccaaa ccagtggtaa ttgccgaaac aaccgcaacg
    19561 ttggcgaata aagagaaaac aggaacttgg aaaattttgc ataaattaac cgcagaacaa
    19621 gttttggata aaaccatcgt cttattcaac tatgtgtatg aaaacaagaa accctatgaa
    19681 gcaggtgatg atccagtagc gaaagatgct agcttgaaca atcaagccca aactgtcaat
    19741 tgtacggtgg aacgccatgt ttccatccaa acaaaagccc acctagaaga tggttcgcaa
    19801 acctttactc atggggatgt ggtggatatg ttcgatgatg tgtcgattac ccatgatgta
    19861 ctggatggct caaaagaagc ttttgaaaca attctgtatg cattacttcc ggataatacg
    19921 accaaagaaa tttggaaatc tggcaaaatt gattatgaag tgaatgacaa agaattcact
    19981 aaaaccgtac ttgcggaaaa agtggacaca agtaaatatc cagaaggtac aagttttacc
    20041 ttcaaagaaa ttaactatgg caaagacgga aacatcaatg ggaaacacaa tgaggatcta
    20101 aaagaaaaaa ctcaaacttt aacacctaaa aaagttccga caacaccaag tgcaccagaa
    20161 caaccggaaa taccaactgt tccaagtgac tctcaagaat ctagtcctac agtaaagaca
    20221 ttcccacaaa caggggagaa aaattctaat gttctgctgt ttattgggtt taccttgatc
    20281 tttgcgactg ctgggtatta tttctggaat cgccgaaact aaggtgatgt gatgaaacaa
    20341 ctaaatagga aaaaccaacg tgcgcgaccg cctcctaaaa aaagtggtcg cgctattttt
    20401 gtatccaaaa ctaaaaatga aaagaggaaa agaagatgga attgaaattt gttgtgccag
    20461 atatggccga aacctttgga aagataaggt atgccggcga aggcgaagtt ttaactgaag
    20521 gatatgggcg gaatactaca gtaattggtc gtagttatca tttgtattcc agtaagcaac
    20581 gagccgatga tattgaagtg gtggtagctg cggaagccgg cgaaaaggat tttgatcaag
    20641 atcaaccact aaaagccgtg aatccccatt tggttgccaa aggctatgag attgagaatc
    20701 gtgggtttac cgactacgtg ttgtatgtcg atgatttagt gaaagcatag gaggaaaaga
    20761 agatgagatt aacagaaggt attgttgtag attcaggact aacgtttggg aaattacgat
    20821 tttccgcatt acgccgagaa gtacggaagc aaaatgagga tggaacggtt agtaacgagg
    20881 taaaagaacg gacttataat ttgaaatcct ctgcgcaagg tcgaatgatc caagttagta
    20941 tcccagcaaa tgtaccctta cgcgagtttg cctatgacgc agaggtagag ttggtcaatc
    21001 caatcatgga tacggtcgcc aattatgttt tccgagaagg aactactgtc aattggttca
    21061 ttaaggccga tgatttagtc ttgaaacgac aaccagatca gggaaattct acaaaccaaa
    21121 atgaaggaaa gaaataggtg aagcgtatgg aaattaatgt tgaacatatt ttagattgtc
    21181 tcgatcaata tggaaaaggt gagctaacgg aggaacaact cactaaagca ttaacctatg
    21241 atgaaaaaat gtttctgatt atgcatcaag ggttactgga agtaggtaac gatgtcgaag
    21301 aagattttga tgtactgaat tggttagggg aagaagagtc cttctttatg gttatcgaag
    21361 taaatgaaag tctatgtcga gaagccgaaa ccgtattgga agaaattggt gtggaaatgc
    21421 cggatgccat tgaagttttt ttaaagcaac tagtggaaac gaaacaactc cctatagcag
    21481 taaatgacta gcataactgc ttgaaattgg atcaatgaaa tcctatgaag cttttttcga
    21541 ttggaggtgg aaaccataaa aatgtacaaa gggcatcgta tacgggcagg agatcagcac
    21601 ttggtttatc actttgttct tggctggcta ctcgctctgt tcatcggttg gatgagcgtc
    21661 ttttattttc aagagcttag acaatttgat atttctaagc tatcactttc tactatagaa
    21721 atcgttcgtt ctataaaaga tttaatctat ttgctaggaa gtctcgttct ttcaggaagc
    21781 acgatgttgc tttatataca cttttttcag gatcattggc gcagtttgtg gcatcgacaa
    21841 aagctagccc ggatgattct agaaaatcat tggtacgaag taaaacaaac tcagagtgaa
    21901 ggatttttca aagacctcaa tagtagtcga accaaagaga caatcagtta cttccccaaa
    21961 atctattacc gtatgaagga tggtttactt tctattcgcg ttcaaatttc actgggaaaa
    22021 tatcaagatc agcttttaaa gctggaaaag aagttagaaa gtggtctata ttgtgaacta
    22081 gtagaaaagg aactcaaaga ctcctatgtg gagtacactt tactgtatga tataattgcc
    22141 aatcgaattg ggatagacga agtagtggca gaaagtgggg ctttgcgatt gatgaaaaat
    22201 caagtatggg cttatgattc tttacctcat atgttaatcg ctggcggcac aggtggtggg
    22261 aagacttact ttttactcac tatcatcgag gctttattaa aatctgatgc cgagcttttt
    22321 gttcttgatc ccaaaaatgc ggacttagcc gatttaggta cggtaatgcc tcatgtctat
    22381 tcccaaaagg aggaaatttc tgcttgtgtg gaagatttct atgagcgcat gatgactcgt
    22441 agcaaggcaa tgaaagaaat gcccaactac aaaacaggag agaattatgc gtttcttgga
    22501 cttccaccaa actttttaat ctttgatgaa tacgtggttt atatggaaat gttaacgacg
    22561 aaggaaagtg cggtgatttt gaataagctc aaacaaatcg tgatgttagg tcgtcaatct
    22621 ggtttctttc tgattctagc ttgtcaaaga ccggatgcga aatatttagg agacgggatt
    22681 cgcgatcaat ttaatttccg ggtggctttg ggtcgtatga gtgaactggg atattccatg
    22741 atgtttggcg aggttgataa aaaattcttt atgaagcgta tcaaaggtcg aggctatgta
    22801 gatacgggag ggagtgtgat tagtgagttt tatacgccac ttgtaccaaa aggatatgat
    22861 ttcttaaggg aaattagcga cattggtgga ttaaacgtta atacggaagg ataaaataat
    22921 agtatgtagc ctaggagtgt caagaatcct gggctttttt tacgaggtga tttatgacat
    22981 ttttagattt attcgctggg attggtggct tttgcttagg tatggaacaa gcgggtcacc
    23041 agtgtatcgg cttttgtgaa attgatgatt ttgctcgcca gagttataag gcaatccatg
    23101 atacaagtaa ggaggtggaa atgcatgaca tcacaagtgt atcagacgag tttattcaat
    23161 ctctcggacc agtggatatc ctttgcggtg gatttccgtg ccaagctttt tcaattgcgg
    23221 gaaagcggca aggattttcc gatactcgag gtactttatt ctttgaaatc gctcggttcg
    23281 ccgctattct ccaacctaag tttttattcc ttgagaacgt ccggggatta ctcaatcacg
    23341 aaggaggggc tacgttcgag acgatcctcc gaacgctgga tggattgggg tatgatgtgg
    23401 aatggcaagt gcttaactca aaggcctacg ttccccaaaa ccgtgagcgg atcttcctta
    23461 tcggacattc tcgagacgca tgtaccgaac aagtatttcc tatcattgga tcgtctccaa
    23521 catctgatca aaacatcaga aacttattaa atatcaaccc ttccaatcga ggaatggggg
    23581 gacaagtcta tgggtcagat gacgtagcgc ctaccttgac cggtgatgaa ggaatcaaaa
    23641 tcgcattgcc ggtagatgat ggaatttctg tagcgggtat gttactggga aattttgaac
    23701 aaggcaatcg ggtttatgag ataactggaa cagcgcctac tttgtcaacg aaacaaggcg
    23761 gaacgaagat catgcttcgg aaaaacgaaa cagaatatca agaggtgaag ccagggaaca
    23821 gtgtgaacct ggcttttcct aattccacaa gtcgtcgagg ccgtctggga aaacaaagtg
    23881 tgcatacctt attaacagga gaccaacaag cagtcgtcac ggatcagtat caaattcgaa
    23941 agttaacacc tagggagtgc tggcggcttc agggctttcc ggactgggct tttgatcgag
    24001 cttctcaagt caattcagat agtcagttgt ataagcaggc tggaaattct gtaacagttc
    24061 cggtcatttt tgatattgct agaagattaa aggaggtaaa aacagatgat ttatgatgag
    24121 ttgattgggg aatctactgg gtgattggca agattcagtc agatccggaa ttggaagaag
    24181 aactgtaccg gctcaatttt gatattcgca aaaacggggt gaaagtccct gatgatccgt
    24241 atgtaattga tgaggaaacg gatgctcgaa tggagctgaa tcaagtcatc agtgaattgg
    24301 aacgaattgc ggactttgct aaagaacctg atatccgcca atacttgttt gagataaaag
    24361 cagagttgga aattgagggt attaccgcgg agtaacggcc aaataaacca ctgataggca
    24421 ctgaccgcgg gggatttgcg aagcagaaat cctccgtggt gctgtgcctg tttatggacg
    24481 gcgttagccg gccatgatgt ctcacccccc gtatctaaca ggggggtaca aaaacacaaa
    24541 aaataagcaa gcaacgataa ggaatggctg tcctatcaag gataataagc aatttgtgta
    24601 gatgttaact gatttgaaaa agttaacacc ttaaatttgg aggtgagaac ttggcacaaa
    24661 ggaatttaga ttatcggcta ttaaaagacc ggcgtaatga atatggcgtt tcacaaaata
    24721 aattagctac gacttgtgga cttagtcgcc cgtacttaaa tcagatagaa aatggtggcg
    24781 taaccgcatc gacaaaaacc atgaggaaaa tttttgacca actggaaagt ttcaatcccg
    24841 agttaccttt aacgttactg tttgactatg taaggattcg ctttcccaca acggatgcga
    24901 ggaaaatcat tcaagagatt ctccatttga aatttgatta tatgctccac gaagattacg
    24961 ccttttactc ctatcaagaa caatatgtta tgggagatat tgtggtgatg ttgtcacatg
    25021 aagaagataa aggcgtcctt ttagaattaa agggtcgtgg ttgtcggcag tttgaaactt
    25081 ttttactcgc ccaaaagcgt agctggtatg actttttcga agattgtcta aaagccggtg
    25141 gcgtgatgaa acgcttagac ctggcaatca atgatcgagt tggcctgtta gatattcctg
    25201 atttaacgaa aaaatgtcag aaggaagaat gtatctcctt gtttcgtacc ttcaaaagct
    25261 atcgttccgg ggaactgttg aaacctgatg ataaagatgg tatgggcaat actttgtata
    25321 ttggcagtct taagagtgag gtctatttct gtttgtacga aaaagactat gagcaatata
    25381 tcaagttagg gattccttta gaccagaccg aaacgaaaaa tcgttttgaa atccgactga
    25441 aaaatgatcg agcttatcat gcaattcaag atttgttaaa aggtcgcagt attgaaagta
    25501 ccacattttc cattattaac cggtacttgc gatttgcgga taaggtggaa ggcaaaaggc
    25561 gaactaactg gcccttgaat gaacaatggg ggcgcttcat tggacggaat cagaaggaaa
    25621 ttcaattaac ttctgaaccg aagccttata ccattgaacg aacgttgaac tggcttggac
    25681 gacaagtcgc ccctacgtgg aagatggcca aggagttgga tcggttgaat cagacaacgt
    25741 acattcagga tatggtaaat aatactcgac tatcagaccg gcacaaaaag attttggagc
    25801 aacaaagtat ggcaattgaa aatttgatta tatgaaaaga ggaatcgtga tggaaattgt
    25861 gaaagatatt ttattggtgt ttggtggcag tatgcttggt gttactgtga tgtgcttgat
    25921 gcatgcaagt gcagcggcgg accgagcaat ggaaaagaaa ggaaagtgat gaaatgaatt
    25981 tcggtcaaaa ttt
//
LOCUS       DAZKNH010000032        25698 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000032 DAZKNH010000000
VERSION     DAZKNH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.032"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            130..3657
                     /locus_tag="ACG8MG_001254"
     CDS             130..3657
                     /locus_tag="ACG8MG_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFQ0375310.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3654..7376
                     /gene="addA"
                     /locus_tag="ACG8MG_001255"
     CDS             3654..7376
                     /gene="addA"
                     /locus_tag="ACG8MG_001255"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFQ0375311.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7445..7786
                     /locus_tag="ACG8MG_001256"
     CDS             7445..7786
                     /locus_tag="ACG8MG_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFQ0375312.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8095..9138
                     /gene="pheS"
                     /locus_tag="ACG8MG_001257"
     CDS             8095..9138
                     /gene="pheS"
                     /locus_tag="ACG8MG_001257"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0375313.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9143..11563
                     /gene="pheT"
                     /locus_tag="ACG8MG_001258"
     CDS             9143..11563
                     /gene="pheT"
                     /locus_tag="ACG8MG_001258"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFQ0375314.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11733..12743)
                     /gene="trpS"
                     /locus_tag="ACG8MG_001259"
     CDS             complement(11733..12743)
                     /gene="trpS"
                     /locus_tag="ACG8MG_001259"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFQ0375315.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12997..13734
                     /locus_tag="ACG8MG_001260"
     CDS             12997..13734
                     /locus_tag="ACG8MG_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375316.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13748..14563
                     /locus_tag="ACG8MG_001261"
     CDS             13748..14563
                     /locus_tag="ACG8MG_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0375317.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14576..15223
                     /locus_tag="ACG8MG_001262"
     CDS             14576..15223
                     /locus_tag="ACG8MG_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0375318.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15239..15895
                     /locus_tag="ACG8MG_001263"
     CDS             15239..15895
                     /locus_tag="ACG8MG_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0375319.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16057..16878
                     /gene="racE"
                     /locus_tag="ACG8MG_001264"
     CDS             16057..16878
                     /gene="racE"
                     /locus_tag="ACG8MG_001264"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFQ0375320.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16881..18236
                     /gene="rph"
                     /locus_tag="ACG8MG_001265"
     CDS             16881..18236
                     /gene="rph"
                     /locus_tag="ACG8MG_001265"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFQ0375321.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18237..18755
                     /locus_tag="ACG8MG_001266"
     CDS             18237..18755
                     /locus_tag="ACG8MG_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0375322.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18862..19362
                     /gene="cbpB"
                     /locus_tag="ACG8MG_001267"
     CDS             18862..19362
                     /gene="cbpB"
                     /locus_tag="ACG8MG_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFQ0375323.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19642..20562)
                     /locus_tag="ACG8MG_001268"
     CDS             complement(19642..20562)
                     /locus_tag="ACG8MG_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFQ0375324.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20688..21740
                     /locus_tag="ACG8MG_001269"
     CDS             20688..21740
                     /locus_tag="ACG8MG_001269"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFQ0375325.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21850..22605)
                     /gene="fabI"
                     /locus_tag="ACG8MG_001270"
     CDS             complement(21850..22605)
                     /gene="fabI"
                     /locus_tag="ACG8MG_001270"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFQ0375326.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22825..23265
                     /gene="fabZ"
                     /locus_tag="ACG8MG_001271"
     CDS             22825..23265
                     /gene="fabZ"
                     /locus_tag="ACG8MG_001271"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0375327.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23380..24141)
                     /locus_tag="ACG8MG_001272"
     CDS             complement(23380..24141)
                     /locus_tag="ACG8MG_001272"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0375328.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24330..25424
                     /locus_tag="ACG8MG_001273"
     CDS             24330..25424
                     /locus_tag="ACG8MG_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFQ0375329.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25510..>25698)
                     /locus_tag="ACG8MG_001274"
     CDS             complement(25510..>25698)
                     /locus_tag="ACG8MG_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0375330.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
BASE COUNT         8509 a         4455 c         5417 g         7317 t
ORIGIN      
        1 ttgcgtatac ttaaaaaagt agaaatatca gaaccttaaa aggatataat ttgaagaata
       61 tagaacaaat gttcagtatt ttttgatata ataaaggagc accgtatcat gtgttgaagg
      121 aggaaaagca tgtctttaca atttattctt ggaaacggga aaaaagatca ccgtcaagtc
      181 ttgatcgacg aagcatccgc ttggctcgac caggatgcaa gtaaccaagt atttttcctt
      241 gttcccaact acagcaaatt cgaacaggaa caagaaattt tagcggagat gcgaagaaga
      301 agcggaaaaa aaactttcag cacaacaaac gttcaagtat ttagttttta tcgtctggct
      361 tggtatttgc tgcaacagac aactcttctt acaggaaatg aattatcaga atcaggatct
      421 gcaatgatct tgcgacagat tttagaaaaa aatgaggaag aattaacaat ctttcgcgga
      481 gaaatcaata aaagagggtt tattcgtcaa ttacaagaac tctacagtga attgcgtaca
      541 ggaaatattc aaccagaaga cctgatgctt ttatttggtg attcacctaa tgcagaagac
      601 cagcaactta aaatgaaaga cttgaagctg attttttcag cttatgacag cgaattgact
      661 caacgtgcac taacaaatga agatgcacta tctatactag caaattatat gcagacacaa
      721 gatttcaaca acgtcaaatt tattgtcagt ggtttttctc gaatcaatgg tcaagagtat
      781 caattactgc aggcaatgat ggaaaagggg caattggttg tcgatttatt gctggatcgt
      841 ccttatacag cggatcttcc agaagtactt acgctctttc atgagactgg aaagctttat
      901 ttccgtcttt ttcaaagtgc aagagaacgg aaagtcccta tattagtgga ccgatttgca
      961 ccagagcaga ctcgtccgac agagcttcaa ctgctgcaaa caatgtggga agagacatct
     1021 agccaaaaga aagcggcagt acacaaaata gggggacaag agcatctcca tttgtgggaa
     1081 gctgaaaatc caactgaaga agtccgacaa ttagctgttg aaatcagacg actagtcagt
     1141 gaagaaaact accgttatcg tgatatccaa gtgctgacaa aaaatatgtc tttatatgga
     1201 aatatccttc ggccgatctt tcaagaaatg gcaattcctt tttatatcga tgaagaacaa
     1261 atgatggaaa atcatccgct gatcgaatgg atcaacagtc tgttcgctct tgaccgctat
     1321 tcttatcggc taagtgatat tatgcgtttt ttcaaaactg aattatttct gtcgacggaa
     1381 gcagaaaccg aacttgatga atggaaacaa gagcgagatg aattcaggaa aaaactggac
     1441 atcactgaaa atgtagcact tgcttacaat tatcaaggaa cttattggac tcgtgaaaaa
     1501 gactggcagt ttgtggcgta tgattttgaa gcagaacaga ttgaagacgt acagtatttg
     1561 gaagaacagt caaatacgat tagacgatta tttcagaaag ctgtaccagt atttttgaaa
     1621 aaaataaaga aaagcaaaac aggacaagag gccgcaaatg ttttttatca atttttgatc
     1681 caaagtggcg tacagcaaca attattgttt tggcgtaatc aggaagtaga cagaggaaac
     1741 ttagaagcgg caagaaacca tgagcagaca tggggagcgc tgattgatct gttggatgaa
     1801 tatgtagtaa tttatggtga atcggctttt gactggtcac tgttccaaga aatcattaca
     1861 agtggtctag aaaatctaag ttatggtaaa atccctaccg caatcgacca agtgcgaatc
     1921 aatcgtttgg aattagttcg accaaatcaa gctgccgtca cttttgcatt agggttgaat
     1981 gatcaagtgt ttccagaccg aaaagagaca aaaggattgt tgtccagtga agaacgagaa
     2041 gtattaaatc aggccttgcc agatggacag tttttattcg acccatcaaa ggaaagtatc
     2101 tcttttgagc ctttccaagc ctatcttgtt tttctttccg gaacagatcg attatatctt
     2161 agttatgcac aaagctatga cacagatggt tcgctgaaga tgtctcctta tttgcgtaga
     2221 atcacagaat atttatctgt tcccgtcgag agaaaagagc atttgacgat cgaaagcgac
     2281 cctaattgtt atgtagggac ttacagaagc ggaatcaata cagtcaatag gatttatcga
     2341 ttagctttag atgaaaaaaa acctgtacca aagcactggc aaaagctcaa agaattagta
     2401 ctgaattcgt catggcgaaa ttttgcttta cagatttttg aaagtcagga acatttaaat
     2461 gttcctgttt cgatgccaga aaaaatggcg gaagagcttt atggaaaaga gatttatact
     2521 tcaatttcac gtatggagaa tttttataat tgtgaataca aatacttcat gcaatttggt
     2581 ttgaaattga aagaacgaac tgtttatggt ctaactccag cagcaactgg tgatttttat
     2641 catgaatcat tggatcgttt cttcaagcta ctgttttcaa accaattatc cttagtgaat
     2701 atgtccgatg cgcagcgtag agactttact gaaaaagtgc tgcaggaagt attcggagaa
     2761 cttcggtttg agatattgga tagttctgca agaatgaact atatccgcta ccagttagga
     2821 cagacgattc aaaaagtgac atgggctttg caaaaacaga gcaaaaatac aggcatgcat
     2881 cctcttcaga cagagattct atttggtcag attgctggag caaaaggtat cacaggtctg
     2941 gaattaccgt tgagtaatgg tggaaaactc catgttcgag ggaaaatcga tcgaatcgat
     3001 gtagcttctg aacaagaaga tacttggcta agcgtcgttg attacaaatc tagcggacgt
     3061 tcattcgatg tcacagaagc ttattatggc atggctatgc aattattgac gtatctggac
     3121 gtagcattga tggatgcggt acgtttgact ggaaaggcgg ctgttaaacc agcaggatca
     3181 tattatttac atgttcataa tcctgtattg acggagacag atgatgtaga gaagaataca
     3241 ttgaaaaaat ttagttatga tggcgttttt gtcgatgatc ccgctctttt agaagtacta
     3301 gatcattcac tggaagcaaa agaacaatca ctgatctatc cagtgaaaaa aaatgcgaaa
     3361 gatgaatacc aaaaaggtag tttcagtaaa gacaaatttt tcacggaaga agaactgcaa
     3421 ctattcatgg cgcataatcg ggaaaatatg cggttagctg gagatgcgat tacttccgga
     3481 gaaatcaaac taaatccagc ttataaagat aaacagcgga ttgcctgcga attttgtcca
     3541 ttcagaagca tctgtacgtt tgatgtgatg ctgaaagaaa ataattacca ccgtctggag
     3601 aaaatagata aggaagaagc actgcgtaga atattgaaaa ggagtgaaga agagtgaatc
     3661 aaattccttt aaaaccgcaa aatgaacgat tcacagatga tcagtggcaa gcgatttttg
     3721 atcaaggaga taatctatta gtctctgcgt ctgccggatc aggtaaaact acagtgcttg
     3781 ttcgaagagt gattgaaaaa ctaaaaatgg gttttgatat cgatgaatta ttgattgtga
     3841 catttactga agctgccgca agagaaatga aagaaagaat tcaagaggca ttacaagaat
     3901 ccgtcaatag cgaaagtgac cctgtacgta gacagcattt tactaagcag ttagttttat
     3961 tgcccacagc gaatatctca accttacatg ctttttgcct gacagttatt cgacgttatt
     4021 attacttaat tgatattgat ccagtctttc gcatgttaac agatgaaaca gaaacgatct
     4081 tgatgaagga ggatgtgtgg gacgagctga gagaagcact ttatgcagaa aatgatgaac
     4141 gatttttcca attaacaatg aatttttcta atgatcgttc agatgacggc ttgacgaatc
     4201 tggtgttttc tctttatgag tttgcccgtg ccaaccctga cccccaaaaa tggctagaac
     4261 agttaagcga taattatcgt ttgcctgagg ggctagcgaa atctcgatta tatcaaaaac
     4321 aaatccgtcc tttagttttg gcagatatat accaatgtgt acagttatat gaacagatga
     4381 ctcagttagc acaaggtgaa ggattagaaa aaatgcatga gcaagtagct ggagaacaac
     4441 agcagatcaa gaatatctat gaagcgttct cgcaagatcg gttagaagaa gcttatgctg
     4501 gtttggaaca actaactttt tcaactttta agagcagtcg gaaagcagaa ttgaaagaaa
     4561 tttccaatga agtcaagggc atgagggata aagcaaaaaa actgattcag cagataagta
     4621 aaagttattt tcctgtttcc ccaagccaaa tggaagaatt aacggacaaa gcactaccgt
     4681 tagtcgaaga aatgacaaaa gtcacgcaaa gcttcatgga cggtttcagt atgcgtaaac
     4741 gggaaaaagg tgtgctggat ttcaatgatc tggaacattt agcgttgcag attctcacag
     4801 aaaaaacgaa agatgtatgg ctgccaagtg aagcttcaaa acattatcga aaaaaattca
     4861 aagaagtaat ggtagatgaa tatcaagacg tgaaccaatt acaagaagcc attttatact
     4921 ggctaagaga acccgatgat acaaaaggaa atatgttcat ggttggggat gtcaaacaat
     4981 ccatctattc tttccgttta gcagatccta gtttatttat cgggaaatac gagaatttct
     5041 caaagaaaga aggcggacgt cgaatcgttt tggctgagaa cttccgctct agaaaagaag
     5101 tcctttcttt tacgaatctt atttttgaac aactaatgga tccagcagtg ggtcaaatca
     5161 attatgacga agcagccaaa ctgatccaag gattttcaga ctttccagag aatgaacagt
     5221 ttgagccgga aatcatgatc tatgaaaaag aacaggaaga atcagagatt gagattccga
     5281 cggatgatat actggaagac aaaacagaag gcgaactttt tatgacagga ctaaaaattc
     5341 gtcaattaat tgattcctcc tttatgattt atgataaaaa atcaaaaaaa agtcgtccga
     5401 tagagtacaa agacattgtt ctcttgacac ctacaaagaa gaacaactta acgattttag
     5461 agattttcaa aacattggat attccattag agatgaatga tgctcaaaac tattttcaag
     5521 cgacagaaat tcgcacgatg atctcgctgc tacaattaat cgataatccc tatcaagata
     5581 ttccgcttgc ggctgtattg cgctcaccga tcgtgggtct gattgaacca gaattagcca
     5641 gcattcgatt agctaaccgt gcacatactt actatgatgc tgtgcttgct tatcaagcat
     5701 caaacgaaga tgaattagca gctaagctgg aacattttgg caagcaacta gagcattggc
     5761 gagaacttgc tcgccgttct tcgatcactg acttgttatg ggatatctat tatgagacgg
     5821 gatatctaga gtatgtggtc ggtctgccag caggggcaca gagacaagcc aatctttatg
     5881 cattggttga tcgtgccaaa gcttacgagc aaagttcttt cagaggatta taccagttcg
     5941 tgcgtttcat tgaaaaaatg caagaaaaag ataaagactt agcagaacca gtcatttcaa
     6001 tagaagataa tgctgttcgc gttatgacga tccatgcaag taaaggcttg gaattcccag
     6061 ttgtattttt gttagatatg acaaaggagt tcaacttaca agacttgcgc aatcggtatg
     6121 cttttgaaga aaaacttgga gcaggaattc gctatatgga tcctgaaaca cgagtgttat
     6181 atgatacgtt accgtttcaa gccatcaaat tggcaaagca aaacaaattg ttgtcggaag
     6241 aaatgcgtaa attgtatgta ggattgactc gggcagaaca aaagcttttc attgtcggtt
     6301 cctataaaaa taaagagcaa atgatccaaa cgtggtctga agcagctgat catgaggagt
     6361 tagtttttga tcctgcgcta cgtttgaagg gcagaagcag cttgatgaat tggattggtt
     6421 acggattgat ccgtcatcct gaaatgcaga aatacttaga ggaagaaatt tcgacttctc
     6481 ttcttcaaca ttcaaatgca cagttttcta tttcatggat gaatcagcaa tcgatcattg
     6541 aacaaagaca gttgcttgct gaaaaagaac tggttaactt agatcagcag atgaaagaag
     6601 atgaaacttt attagcagat tcgttacaaa aacgtctggc atatgaatat ccttatcaag
     6661 cttcttcaca aaccacaagt tatcaatctg tatctgaaat caaacgatta ttcgaagatc
     6721 cagatgatac acaagaatca cgtttgactc tggaaagcag tcagaacaaa gcagcatccc
     6781 gtcaatttcg ttatacgcaa gaacaactag cagaaccaaa atttttgcaa aaagatcggc
     6841 aagtgtcagc agctgctgtt ggaactgcta cgcatgcttt gttacaatta ctgccattag
     6901 agatgccaac tacagagagt atccatcaaa aattacagga attggtaagg aaacggttag
     6961 tagacgaaaa agtcgcgaaa aaagtagatg tttcttctat tatatggttc tttcaaacag
     7021 aacttggtca acagctgatt gccaacaaag aaaacgtaaa acgagaacaa cctttttcca
     7081 tgctgcttcc tgctgacgaa gtatttcaag actatccaaa tcaagaagat gaactattaa
     7141 tccacggaat cgtcgatgga tacctagaag aaaaagatca tctcaatatc tatgatttta
     7201 aaacagattt tattttacat ccggatgatc cagcagaaat agatgcaatc gtccaaaaat
     7261 atcaaggaca gctaagactt taccaacaag caatgtcaga agctttgaat aagccagtag
     7321 aaaatgtttt cctaattttg cttcgagtga aacaaattat aaacattaac aaataattat
     7381 aattgagcaa acttgactta tgttttgtaa ggattatgct atactctggg aagaggtgag
     7441 tatgatggag ctaacacaga cagaattcgt tttatgccct aaatttgaaa aaacattctc
     7501 tattttagga aaaaaatgga acggattgat tatcgatgta ctactcgaaa acggtccgca
     7561 acgctttgtt gagcttgcgg caaaaatccc gatggtcagt gatcgagtat tagtcgaacg
     7621 cttgaaggaa ttagaaaaag aaggaatagt cactcgaaac tttgcctgta gcgaaaagag
     7681 ccgttcagaa tatctcttga ctgaaaaagg agcagacttg caaaaagcga tgcgtcaaat
     7741 acaagtttgg agcgaaaaat ggatcacaga ggaagaatgc ggttgatttt gtaaaaaaaa
     7801 tcttgtaaac tatagtccat acgaataagt aaagaacgat gacgaaaaag agtagtttgg
     7861 ctaaaccagt tcagggagat ctgtcgcaga ctgaaagcag atcctgtgtt tgaaaaatga
     7921 agttcacttt ccgagttcgt ctgataaaat cagaccggca attgtcgtta acgaagtaag
     7981 agtgcggtgt aagtcattta tccgcaaatt aggatggtaa cgcgaagacc tcgttcctta
     8041 acaggaatga ggtctttttg ttttgtattt ggaatttaaa ggaggaacaa gcacatgtct
     8101 ctacaagcac aattagagag cttgaaagaa gcaacgctcg tcaaaatcac agcggcacaa
     8161 gatttaaatg aattaaatca aatacgtgtt gaaacgttag gaaaaaaagg accgatcaca
     8221 gaagtattac gtgggatgaa agatctaagt ccagaagagc gaccaaaagt cggaagtttc
     8281 gcaaatgaga tccgagatct tttgacagaa gagatcgagc gaaaaaaaca agtattagaa
     8341 gaagaagcgt taaatgcagc tttggcaaaa gaaacaatag acgtgacact acctggtaga
     8401 aaagtaaaac atggtacacg tcatatcgtg acacaaatca tggaagaaat tgaagatatt
     8461 tttgtgggta tgggctatca gatcgtagaa ggatatgaag tagaatctga tcattacaat
     8521 ttcgaacgga tgaatctgcc aaaagatcat ccagcaaggg acatgcaaga tactttctat
     8581 atttcagacg agatattgat tcggacacat acttcaccag tccaagcacg gacaatggaa
     8641 aaacatgatt tctctaaggg tgctctacga atgatctcgc cagggaaagt cttccgcaga
     8701 gatacagatg atgcgaccca cagtcatcag ttccatcaaa ttgaagggct agttgttgat
     8761 aaaaacatca caatgggcga ccttaaagga acattagaag tcgtaatgaa aaaaatgttt
     8821 ggggaagatc gtgaaattcg tttgcgtcca agttatttcc catttacgga accatcagta
     8881 gaagtagatg tcagctgttt caaatgtggt ggtgccggtt gtaacgtatg taaatacact
     8941 ggatggatcg aaattttagg agctggcatg gtacacccga atgtgttgaa gatgtcagga
     9001 atcgatccag aagaatattc aggctttgct ttcggccttg gaccagaccg cgtagctatg
     9061 ttgcggtatg gagtgaatga tatccgtaat ttctatcaaa atgacttacg tttcttaagt
     9121 cagttcaagg gggagtaaga aaatgttagt ttcatataaa tggctcaatc gttatgtaga
     9181 tttatcgaat gtaacaccaa aagaattagc ggataagatg tctgtgacag gaatcgaagt
     9241 agagggagta acggttccag aagaaggctt gaaaaaaatc gttgtcggtg aagtcaagga
     9301 atgcgtacca catccagatt ctgatcacct ttctatttgt caagtcgata ttggcgaaga
     9361 cgaactgtct caaatcgttt gtggagcgcc taatgtgaag gttggaatca aagtgattgt
     9421 tgcacttcct aactcacgta tcgctggtaa tgtaaaaatc aaaaaaggaa aaatgcgtgg
     9481 ccaagtatca aatggcatga tttgctcatt acaagaaatt ggctattcag atagtgttat
     9541 accgaaagaa tacgctgaag gtatctatta tatgccacaa gaagctgtaa atggcgatcc
     9601 agtttttcct tatttggata tggatgattc gattattgaa ttatctatca caccgaatcg
     9661 tgcggatgca ttgtcaatgc gtggcgtagc ttatgaagtt ggggcaattt atcgtcagac
     9721 tcctacattc gatgacgaaa agctggtaga atcctcacgt cctgctagcg ataaaattca
     9781 agtggaagtt gaagataaag aagcagttcc tgcttatcaa atcagaatca ttgaaggagt
     9841 aagaatccag ccaagtcctc aatggctgca aaatcttttg atgaacgaag gcattcgtcc
     9901 aatcaacaat gtagtagatg tgacgaacta tattttgctt ttattcggac aaccactaca
     9961 tgcatttgac tacgacaaat tgaaatcaaa agagatcttt gtccgccgtg caaaagaaaa
    10021 tgaaacatta gtaacactag atggagaatc tcgggaattg acaactgaca atctcgtcat
    10081 tactaacggc aaagaaccag ttgctttagc tggtgtcatg ggagggttag actcagagat
    10141 cacgaatgaa acaacaacag ttgcactaga atctgcttta tttgatccaa tttcgattcg
    10201 tcgtacagca aaacaattta atttgcgtag tgaatcctct gcacgttttg aaaaaggaat
    10261 caaccatgcg actgttggag aagccagtga ggttgctgca gcaatgatcg tttctcttgc
    10321 gggtggggaa gttctacaag gcgcggtcaa aggctcagag atcaaagcag aagacgtatc
    10381 agtgaagatt tcacttgaac gcatcaatca atatttagga acatctcttg tcgttaaaga
    10441 agtaaatgag atttttgaag cacttggatt tagctatgaa gaaaaggagt atgtttattc
    10501 cgtagtagta ccaccaagac gctgggatat ccaaatcgaa gcagatatta tcgaagaagt
    10561 tgctcgtatt tatggatacg accagttgcc atctacactt ccaagtggag aaacggttgc
    10621 tggaagcttg tcaaaagaac aagaaactac tcgtaaaatt cgtacaatct ttgaaggaag
    10681 cggattgagc gaagcaatca gttatgcatt gacaacagaa gaaaaatcaa gacagtttgt
    10741 tataaatgaa agtaatatta cacgtttgga ctggccaatg agcgaagaac gttctgtatt
    10801 acgaatgaat ttgatcagcg gactattaga tgatatcagt tataacactg cccgaaaaaa
    10861 tacagagatt gctttatatg aaattggtcg ggtgttcttc caagaaaatg atccacgtac
    10921 acatctgccg caagaagtaa aacatgcagc aatcgctatt tctggtattt gggaagaaaa
    10981 agattggcag acgaaagctc aaaatgtaga ctttttcacc atcaagggtc ttttagaaaa
    11041 cttatttgaa caactaggca tcactgaaga tgtccattat caaacagtga cagaaatgaa
    11101 ggaaatgcat cctggaagaa ctgctgcgat ttaccttggc gaaaaattta ttggttttgt
    11161 cggtcaagtg catccaacga ttacaaaaag ttatgatatt ccagaaacat atgttgctga
    11221 gtttgattta tctgctatag tggaagctgc tcaaaaaggg atcgttttcg aagcagtgac
    11281 aaaattccca gcagtcagcc gggacatcgc tttgcttgta aaagaaactg tgacaaatca
    11341 ggaattaaca gaagttatcc gttctgctgc cggaagattt ttaacagata tccaattatt
    11401 tgatgtctat caaggagaaa acattgaaaa aggacataaa tccatggcgt atagcctgac
    11461 attcgtcaac ccagaagcaa cattgacaga tgaagaaatc aataaagcaa tggaaaaagt
    11521 cgaaaaagcg ttggtagaaa acctagaggc aagtattcgc taagaaggaa aatatagtaa
    11581 gtaacgatca agtagctttt gaagaccata catttgaaaa aaagatgtga cacaacttcg
    11641 tcacatctct ttttttgatt ttaaaaaagg aatagcacag agacgagata aaaaaacagg
    11701 cctttacagc ctgctttttt agagtaaatg aattaatgga agtaattgat tcccattgct
    11761 gattttactt cagaaagtgt ttgtgcagct actttagcag cttcttcact acctttacgt
    11821 aagatttcca taacagctga aggatctttt tccaattctt ctcggcgcgc acggatcggt
    11881 gcaaattcag cttccaatac atcaatgagg tatcgtttga tctttacatc accaagtccg
    11941 ccgcggcggt aatgagcttt catttcttca attgcttctt tatctgttcc gaatacatcc
    12001 aagtaggtga agaccatatt cccttctact tgtccaggat cttgtacatg gatgtggttt
    12061 ggatcagtat acatactcat gacttttttc tgtaagacat cggcaggatc ggagatataa
    12121 ataccgtttc ctaacgattt actcattttg ccatttccat cgattccagg taatctaccc
    12181 attccttttg gtgggaatac gccttttggt tccaccaaga cttcaccata tgtatgattg
    12241 aagctttgta cgatttcctg tgtctgctct agcattggtt tttgatcctc accaacaggt
    12301 acaagattcg ctttgaatgc agtgatatct gctgcttgag aaacaggata gataaagaat
    12361 cctgtaggca cgctttcacc gaattttttt tgttcgattt ctgttttgac agtcggattg
    12421 cgccgaacgc gtcctacgct gactaagttc aaataataca tggttaattc agctaattcc
    12481 ggaatttgtg attggataaa taaagtagat tttgcaggat ctaatcccac ggctaaatag
    12541 tctaaagcaa cttgcaagac atttgatgaa accttctcag gatttttagc attatctgtc
    12601 agtgcttgca tgtcagcaat catgacaaac agttggttgt cttcatctgc ctgcatagcc
    12661 actcgggttt ttagagaacc gacgtaatgc cctaaatgca gcttcccagt tggtcgatct
    12721 cccgttaaaa taatattttt caaaaaagac acctctttac gatttgtaag tctgagcact
    12781 taatatctta ccgtttttcc tgaaaagaaa caacagttcc taaggaaaaa acaaggaaag
    12841 aggataaaat aactaataga ttagaaatgg aagaaatatt tatgggagaa taaacgttct
    12901 aagaaacttt acaaattact aaactatcag atataatgta ttttattata catctttaaa
    12961 agaaatctta ctaaataata atgggagtgt aattctatgg caatgattaa atttgatcac
    13021 gtggataaat attatggcaa gttccacgca ttaaaaaata tcaatctcga attcgagaaa
    13081 ggagaagttg tcgttgtcat tggtccttct ggttcaggaa agagcacgat gcttcgctgt
    13141 atcaatggat tagagacgat ctcttcaggt aagctgctga ttaatgatac tgacttgcat
    13201 gataaaaaga caaaactgac agaagtccga aaaaacatcg gcatggtttt ccaacatttc
    13261 aatttgtacc caaataaaac tgttctggaa aacatcaaat tagcaccaat caaagtgttg
    13321 aagcaagacc aggcaactgc agtcaaaaac gcggagaaat ttttgaaaac tgtcaatatg
    13381 ttagataaga aagattctta tccttccatg ttatccgggg gacaacagca acgggtggca
    13441 atcgccagag gtttagcgat gaatcctgag atgctgctgt ttgatgaacc tacttctgct
    13501 ttggatccgg aaatgattgg tgatgtatta gacgttatga aaaaattggc tcatgacggt
    13561 atgtcaatga ttgtcgtgac acatgaaatg ggctttgcca aagaagtggc ggatcgtgtt
    13621 atcttcatgg ctgatggaga agtattggaa gacagcagaa atgtgagaga ctttttcgaa
    13681 aatccaaatg aagtccgagc acagcaattt atcagtaaag ttatcaatca ttgataggag
    13741 gaaaaagatg cgcaggttaa aaaaaatacg cctgattttc cttttaggca ttgcttttct
    13801 tgcactagcc ggatgtaaag gaaatgggtt ggcaaatgaa gatattttag agcgaagcaa
    13861 atctaccaat gaaatcatct ggggtgtcaa gtatgatact cgattatttg gaatgatgga
    13921 tattgagagc agaactgtac aaggatttga tgtagatatt gcaaaagcaa tcactaaaaa
    13981 gatccttggt gacaatggaa aaacagaatt tgttgaagtc acttctaaaa ctcggattcc
    14041 attattgaaa aacggaaata tcgatgcaat tattgcaacg atgacgatca cagatgaacg
    14101 aaaaaaacag gttgactttt cagatgtcta tttcgatgct ggccaagctt tgttggtaaa
    14161 gaaaggaagc caaatcaaaa gtgtagatga tttgaatgct tctacaacgg ttctagcagt
    14221 caaaggttct acatcggctg cgaatatccg acagcatgca ccagatgcaa aaattttaga
    14281 attagaaaac tatgcagaag catttactgc tttacaatct ggtcagggag acgcgatgac
    14341 tacggataat gcgattttgt tagggattgc agatgaaaat ccagagtatg agttagttgg
    14401 tggaacgttt actaatgagc cttacgggat tgcgatcaac aaagggcaag aaaacttctt
    14461 gaaagctgtt aaccaagcgc tagaggaaat gcatgcagat ggcacatacg ataaaatcta
    14521 tcaaaaatgg tttccaaatg aaactgaagg aaaagtagag taagggggag aggcaatgag
    14581 cgaattgata caaacttatg gaccaatctt tgtcgacggg ttcaagatca cattgttttc
    14641 gagtgtctta gcacttgtgt ttagtttgat tatcggaacg ttaatggcaa tcttccaatt
    14701 atcaaagaat aaagtagtga gcgccttagc aaaggcatat gtagaatttt ttagaaacat
    14761 tccattatta atcatcgtga tgttttttta tgttgtcgta ccgatttatt ggtttaatct
    14821 agatggattc caagcaggta cgattggatt aacgatctat acatctgctt ttattgctga
    14881 aacagtacgt tcagggattc aaaccgtacc aaaaggtcaa acagaagcag gactatcttc
    14941 tggttttacc tattcagaaa tcatgcgtta tattgtttta ccgcaagcat tcaaaatcgt
    15001 tatcccgcca ttaggcaacc aatttatcaa cctcgtaaaa aactcatcta ttttagcaat
    15061 cgtagctggt ttagatttga tgtatcaagg agacttgatc gctagtgaaa cattcaatac
    15121 cttcgatacg tatatcatcg ttgggttatt ttacttagtt atcacgctac cgttatctta
    15181 cttaatggtt tatcttgaga aaaaatgggc ggttcgttca tagaaggagg ggaaatacat
    15241 ggattttagc ggcgcttttt catggatgaa tatccgtttt ttattggaag gattaaaggt
    15301 tactgttgaa gtttcattgt tttcgatcat ttttagtttc ttgatcggtg gtctgacagg
    15361 tgtgattcgt tttgcgaata tccctttctt ttccaaaatc cttggtgtag tgattgatat
    15421 tatacggaat cttccattat tattgatcat tttcttcact tactttgcct tacctcaaat
    15481 cggcattcag atgaacatct tctggtccgc tgtcgcagca ttaacgattt ttgagtcagc
    15541 tatgttgtcg gaaattttcc gagctggact caatgctgtt ccaaaaggac agatggaagc
    15601 aggattatca acgggactga cttatgtaga atcgatgcga acgatcgtgt taccgcaagc
    15661 atttaaatca atgatcccgg caattgtgag ccaattgatt tcactgatca aagatacttc
    15721 attagcagta atcatttctt tacctgaact ttctcatcag gcacgtatta tttatggaca
    15781 gaacaccaat tatgtgcttc cgatgttcat catcatgaca cttatgtact tcgtggtatg
    15841 ttacgcgctt tctctgcttt ccggttactt ggaaaagaga cggtacagtt attaatagat
    15901 gattctttta gcggttgtgg caaactaaag tcacaaccgc ttttcctctt tccattcgaa
    15961 aaaaagagat ttcgattgtt ttaagcaagt aagagcactt gaaaattcac gctaaaaagt
    16021 acacaatagg gaggatagaa tttattgaaa aaaaggatga tacgattgac agataatcgc
    16081 cctatcggat ttattgattc aggtgtcggc ggcttgactg tagtaaaaga agccctgaaa
    16141 caattaccga atgaaaatat tttatttgta ggagacacag cacgctgccc atatggccct
    16201 agacccgcgg aacaggtaat ccagtatact tgggaaatga cggattatct ggtggagcaa
    16261 ggaatcaaga tgctggtgat cgcctgcaat accgcaactg cggtggcttt agaagaaatc
    16321 aaagctgctc tttctattcc agtcatcggt gtgatccttc ccggtactag agcggcagta
    16381 aaaaaaacac aaaataaaca agttggcatt atcggtacga ttggtacggt aaaaagtcaa
    16441 gcttatgaaa aagcactgaa agagaaagta ccagaattga ctgtgacaag tcttgcttgt
    16501 cccaaatttg tttcagttgt cgaaagtaat gaataccatt catcggtggc gaaaaaaatt
    16561 gtggcagaaa cattagctcc tttaaccact aaaaaaatcg atacattgat tttgggatgc
    16621 acccattatc cattattacg ccccatcatt caaaatgtaa tgggagaaaa tgttcaactg
    16681 atcgattctg gagcagaaac agtaggtgaa gtatctatgc tgttagatta tttcaatttg
    16741 agcaattcac cgcaaaatgg tcggacatta tgccagtttt atacaactgg ctctgccaaa
    16801 cttttcgagg aaatagctga agactggctt ggaatcggac acttaaatgt agaacatatc
    16861 gaattgggag gaaaataagg atgcgtcatg acgggagaaa agcccaagag cttcgcaaac
    16921 tggagcttca aacaaatgtt ttcaaatatc cagaaggatc ggtagtgatc cgttttggtg
    16981 atacaacagt tatttgttct gcaacaatag aaaattctgt tccccctttt ttgagagaaa
    17041 caggaacagg atgggtgaca gcagaatata gtatgctgcc tcgagcaacg agtacgagaa
    17101 atcgtagaga aagcagcaaa gggaaacttt ctggtagaac aatggaaatc cagcgtctta
    17161 ttggtcgttc cttgcgtgca gtagtagact tggaaaaatt aggagaacgt agtatcatcg
    17221 tagactgcga tgtgatccaa gcggatggcg gtactagaac agcaagtatc actggggcat
    17281 ttgtggcatt gaaattagcc attgaaaaac ttttaagaga aaaagaattg tcagaagatc
    17341 caataaaaga acatcttgct gcagttagcg taggaatctt accagatggg acttgcgtca
    17401 cagatttgga ttaccaagaa gattccgcag cacttgtaga catgaatctt gtaatgacag
    17461 aatctggaaa attcgtggag attcaaggaa ctggagaaga agcaacgttt gatggtgaac
    17521 agttgaatga aatgctcttt tttggaaaaa acgcgattga agatttgatc aaagagcaaa
    17581 aacatgcttt gctaacagag tttgctcaaa atgatgagcg gatagaagaa acaaaaacaa
    17641 tcatcattgc gacaagaaat ccaggaaaag cagaagagtt tcgaaatatg ttcaaagaag
    17701 cgggatatca tgtaaaaaca ttacttgact atcctgaatt gccagatgtg gaagaaactg
    17761 gcagcacatt tgaagaaaat gcccgattaa aagcagaaac gattgcacaa ttgcttgatc
    17821 agccagtctt agcagatgat tcaggtttga aagtggatgc tttaggcggt atgccaggaa
    17881 tctattctgc acgttttgca ggggaacaaa aaagtgatgc ggggaataac gccaaattat
    17941 tatatgaatt gacagatgtg ccggatgaaa aaagaacagc acaattccat tgtacgttag
    18001 tattcgcggc acccaaaaaa gacagccttg tagtagaagc ggaatggccc ggtcgagtag
    18061 cccgtatacc atctggcgaa aatggctttg ggtatgatcc tttatttatc ccagaaggta
    18121 agaaacaaac agcagcagaa ttatcgagcg aagaaaaaaa caagatcagt catcgggctc
    18181 aagcaatgaa aaaattgagt gccgagtgga aacaatggct ggaaggagag agatagatgc
    18241 ggtatttagt agtaagtgat aatcatggag acagagatat cctcgttgac ttagtagatc
    18301 gttatcgttc tgaagtggat atctttttcc attgtggtga ttcggaatta gaagcttctg
    18361 acccattatg ggatacattc caagtggttc aaggtaattg tgattatgga ccaggattcg
    18421 aacagaaaaa agtgatccaa actggtcagg atacagtatt catgactcac ggtcatttat
    18481 cgaatgtccg atttggtttg acacagttag caatcgaagc tcgagctgca actgccgata
    18541 tggtgttttt tggtcatacc catcaaatag gctgtgagat ggatcagaaa atcctgtttt
    18601 tgaatcctgg tagcatttct cagcctagag gcccgatcca aatcccatca tatgctctta
    18661 tcgaaagttt agatgaccaa atttctgttc aatactataa ccgtgcccat caggcagttg
    18721 aagagttggc cttttctttt tcgagagagc gctgaataaa aaaactctaa tttttaataa
    18781 aaataagctt tattttatga attttgcatc cggtttcggt aaaatagtag tgttgaatga
    18841 aatagaaatg gaggccatca gatgattgga cctatagtaa gagaaatgct gctggaaaat
    18901 caggaaacgt ttttgattcc tgcagaaaat gtggcaaatg ttatgtatca tcatccattg
    18961 tcccatggtt tacttgtgtt atcgaaggtc ggttattcca aaattcctgt attaggaaaa
    19021 gatgatcact ttgtaggact agtaagttta tcagatgtgg tcaataaaat gattgacttg
    19081 caaacaatca gcatggaccc gttagaagga ctaacggttg ctgatgtgac agaaacagat
    19141 gttccgacaa ttgatgaaaa ttgggaacta gaagaagtac tccatttgtt agtcgattcg
    19201 ccttttttac cggttgtcac agaagatcgg attttcaaag gaatcattac aagaaaagag
    19261 ctgcttaaag ccgtaaactt catggtgcat gaattagaac gtcgtaatat attgtctccg
    19321 aaaatcgaag aattaaaaga acaaatcgat atcgtgagct aaagaacgag aaactaaaag
    19381 ataatcaaaa aataagtaga aaaaacagcg taccagtttt gactttatct tgaaaaagag
    19441 gctgggacaa aagcgtttaa ctccaagaaa taagaaggaa ttcacgaaaa ttgcttttca
    19501 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc cgtttatacg
    19561 tgtttagagc gtgggacaaa agtgttcttt acttttgtcc cacgctcttt ttagtgagat
    19621 tcgttgcatc gtttgtccaa attattttcc gacagcaatt ggactgttcg gaatcgtgaa
    19681 accagaagct gtcaattctt tgacatagga gctcaagaac tcttctttta aggcatattg
    19741 ttttccgttc aatgcgtagc agaccgtacg aatcgcaaaa ttgctgtttc caagatccac
    19801 taatccaaag atagaaggtt cagtttggag ctcttcttgg tatttttcag ctaatgtacg
    19861 atttactcga tcaatgatct catagatttt gtcatatcct tcttcaggaa caatccgaat
    19921 atctaaaagt acttgcatat ttgcacgaga gagattgctg atcgtggtga tgtttcgatt
    19981 cgggatgaaa tggacagtac catccgttgc tttcagctga agggtacgga taccaacaga
    20041 tacgacggtt ccttcaatcg ttaagtttgc tagcttgatg tagtccccaa catccatctg
    20101 ttgttccatg ataataaaaa atccggtaat aacatcactc atgaaccctt gggcaccaag
    20161 accgattgcc acacctgcaa ttccggcacc agccaaaagt gatccgatcg gcacaccgat
    20221 agcagataaa attgcataga taaaaaagaa accaagggta taatgaaaaa tagtatttaa
    20281 aagactatgt aaagtcttta agcggctctc gtttagaatg gcttttttag aatactgatt
    20341 aaaagactta tcaatcagat attttcccac acggtgaaga atccaaaaaa ggacgatcaa
    20401 aaaaatcaga tataaacttt tttggataaa ggcagccaaa atggcgtccc aatcaatccc
    20461 gtcccaaaac cgctgaaacg catttacttt gctgacagct tgatctgtca atgtctcgcc
    20521 agaagagtct gtcgtaccgg cagttgctaa taatgaaaac ataaaatcct cttttctaaa
    20581 atttgattct ttctgtattg taccaagaag ttttcaaaaa aggaaatagc aaaaataact
    20641 ataatgaagg aaaaaagctg aaaataaaat gacaaataat atggataatg ataaaatgtg
    20701 gagaaaataa cgaaggtggt aagtatatgc aaatcgattg gaacaatcta ggtttttctt
    20761 atatcaaaac tccttggcgt tttattgcca aatggaaaga cggacaatgg gaagaaggag
    20821 aattgacaga agataattac ctgacgattc atgaaggctc tccagcgctt cattatggtc
    20881 agcaatgttt tgaaggtcta aaagcttatc gtcgaaaaga cggcaaggtc aatctctttc
    20941 gacctgaaca aaatagtcga cggatgaatc aaagtgctag acgattgctg atgccaatgg
    21001 tccctgaaaa gatgttcgtt gatgctgtca agcaagtggt aaaagcaaat gaagattttg
    21061 tgccgcccta tggaacaggc gctacattat acttgcgccc tttactgatt ggggtcggtg
    21121 aaaacattgg tgtccatcca gctccagaat atttatttgt cgtcttctgt acgccagtag
    21181 gagcgtattt caaaggtgga ctaaaaccaa cgaattttat tgtatctgat tacgatcgtg
    21241 ctgctcctaa ggggactggt gcagcaaaag tcggcggaaa ttacgcagcg agtttacttc
    21301 caggaacaga agcaaaagaa ctggattact cagattgtgt ttatcttgat ccagccacac
    21361 atacgaaaat cgaagaagta ggtgcagcaa atttctttgg aattacaaaa gacggaaaat
    21421 tcatcactcc gcaatcgccc tctatcttac caagtatcac gaaatattcc ttgttgcagt
    21481 tagctgaaga acgactagga ctgcaagcaa ttgaaggaga tattttcatt gatgatctag
    21541 atcaatttgc agaagctggt gcatgtggta cagcggctgt tatttctcca atcggtggga
    21601 tttatcacaa taacaatctt catgtatttt atagtgaaac ggaagtagga ccagtaacaa
    21661 aacggttgta tgatgaattg acaggtattc aatttggaga catagaagca ccagaaggct
    21721 ggattgaagt cgtggaataa tttcaaagta atgagttgaa aaaaacgtaa aaagaaaaaa
    21781 tcaggaagag actttaaaaa gttttttcct gatttttata tgttttactt taaagaatgc
    21841 aacaaaagtt tatgaaagat ggacaccttt gtcaacgtag ataatatcac cgatgattcc
    21901 gctggaaagt gggctgatta agaaagcaca agcattaccg acttcttcga tcgtcacacc
    21961 ttgctgatca ggcgtacgag attcagataa ctggatcagt ttttgatagt ctttaacacc
    22021 tgtgacagct aatgttttga ttgccccagc tgagattcca ttgacccgaa ttcctttagg
    22081 cgagaactca gctgccaaat aacggatagc agcttcaaga gaagctttcg caatacccat
    22141 catgttataa ttcgggattg cgcgttctga acctagataa gtcatgctaa cgatcccgct
    22201 gtttggtgca agaaacggtt gtgcgtattt tgatacagca attaaagagt agctgctgat
    22261 atcttgagct aattgatagc cgtctctgga aatatcagac acatttccgg acaattcttc
    22321 tttgttggcg taagcaactg catggacaag gccgtggatc tccccagcat attcaccgat
    22381 ggcattcatt gctttttcga tgctttcgtc gctagaaaca tcacattcta cagtgaatgc
    22441 ttcttcgccg accaatttga ctaatgattt tttcattcgt tcattttggt aagtatagat
    22501 aatttctgcc ccttgtttca acattgcttc agcacaaccc caagcaatgc tttttttgtt
    22561 agcgacgccc ataatgacga ttttttttcc ttctagaaat gacataatga ctctcctttg
    22621 tttctgataa aaatttgata gtcgtaaaaa aatagagaat aatagctaaa ataacttctt
    22681 gatttcttaa ttattagtca aaatgttttg attgtcaaac tattttttct taaattaaaa
    22741 aaagattgct aaatatttag gaatatgata ctttgatagt caaagtaatg aaaatggcaa
    22801 ttgcctgaaa gaaggagttt aattatgtct tacgtattat ctgctactga agtaatggaa
    22861 ttgattccta atcgttatcc tatttgttat attgattatg tagatacttt agaaccagga
    22921 aaaaagatcg ttgcgacaaa aaatgtaaca atcaatgaag attttttcca aggtcacttt
    22981 cctgacaatc cagtgatgcc gggtgcgttg attctagaaa cactggcaca agcaggttcg
    23041 atacttatcc taaaatcaga agagtttcta ggaaaaagag cttatatcgg tgggatcaat
    23101 aaggccaaat tccgtcaaaa agttgttcca ggagatgtgc tgaaatgtca ttttgaaatc
    23161 accaaaatga agggatcagt cggtacagcg ttatctgcag catatgtaga tgataaaaaa
    23221 gtctgtgagt gcgagttcac gttcatcgtc aaccaaaatg cttgactata aaacctgata
    23281 tagcaaaaca aaaggaagct tacttgcttt ttatgtaaaa agaagacgcc tgaatcctag
    23341 catacgttgt catctatgca ggattcagac gttatttttt tatttttgtt ttgcttcttg
    23401 cagtatattg attttatcga tttttacttc ttcttttggt ttgtctgaac tatctgtttc
    23461 gatatttgca atagcatcca cgacatccat tccttcaatc acttggccga agacagtata
    23521 atggccatct agtgaaggat aaccgccgtt tttataagcg tcgatgattg cttgaggata
    23581 gtcgtctttt aaaagaccat catgcatatc atctgtattt tgaacgataa agaactggct
    23641 gccgttactg ttaggatctt gtgagcgagc catagataac gcacctctta tattataaag
    23701 gtggttgttg atctctgttt caaatccttc tccccaaatg ctttcccctc cagtgccatc
    23761 gcctttagga tcgccacctt ggatcatgaa atctttgatg acgcgatgga aagtcagtcc
    23821 gttgtagtaa ccgtcttttg catgtgtcat aaagttttca accgcttttg gtgcgtgttc
    23881 gggaaataat ttgattttga ttgtgccttt attcgtcacc atttcgacca gatcttcatt
    23941 ttcggcgaca tctttcgtca attgtggtaa ttccaatgcg ttcaaatcga cgctactggc
    24001 agtcgtgctt tccgcagtag atgtactttt tgaactttct gtagacgaac tagaagaagt
    24061 cgtgttgttt gcagaacaac cagcagcaaa cagcaatgct gctgctaaac taatacttaa
    24121 aataacttta tttttcttca taactgttcc tttccaattt actattttgt tatctttaat
    24181 gataacaaaa tagagggaaa atacctactt aaattattgg aatttatcat ttattttaaa
    24241 agaaaaagaa aaggtgtaga ccatttactt tgtgtttgct ataatgtaag tggatacaaa
    24301 aacgaaaaga tgcaggagga aatggtaata tgggaaaatt aggaatctcg atttatccag
    24361 aacggtcaac atttgaaaag gacaaagcgt acttggattt agctcataag tatgggtata
    24421 aacgggtttt taccagtttg ctgcagatca atgatgataa agaaaaagtt ctttcagaat
    24481 ttaaagaagt agttgattac gcgaatagcc taggcatgga agtaatggtt gatatcaatc
    24541 cagcgttatt tgaacagttg gagatttctt acgatgactt atcttttttc cataagatgg
    24601 gtgcctacgg ggtccgctta gatatcggat tcacaggagc agaagaagca aaaatgacgc
    24661 gcaatccttt tggtatcaaa atcgagatca atatgagttc gggcacgaat tacgttgaca
    24721 acatcatgag ttattcgcca aacacggata atttattagg ttctcataat ttttatcctc
    24781 atcgatactc tggtttagga tacgaacact ttgttttttg ttcagaaaaa ttcagaaaat
    24841 ataatttgaa tacgatggca ttcgtcaatt cgcaatctgc tgagtttggc ccttggccta
    24901 cgcaagatgg cttgtgcacg ctggaagacc accgagatct tgaaattgcg acacaagtga
    24961 agcatttaat ccttacagga ttgattgatg atatttctat tggaaatgcg tacgcaagtg
    25021 aagaagaatt gaaagaaatg gcagaagcat ttaacgcgga ttatccaaca ttgaaagtag
    25081 atacggaaga aggtattaca gaaaacgaac gaatctgctt gtttgataac cttcatagtt
    25141 atcgagggga tcgctcagaa tatatccttc gttcaacgat gactcgtgta tactacaaag
    25201 ataaagattt cccgcctcat aatacgcgag atatgcatca tggggatgta ctgattgaca
    25261 atgaagggta cggtcaatac aaaggagaaa cacaaatcgc cttgaaggat atgaaaaacg
    25321 acggacgtgt caatgttgta ggaagaatct ccgatgacga attattcctc cttgactttt
    25381 tgaaaccatg gtcaagcttt aaattaattg aaaataaaaa atagataatt agttattgca
    25441 gaaataatcc gctagaaata gccagttatt tctgcttttt tattttgttt ttttataggt
    25501 tatgatttgt caaattaagc tagaataaaa tgcttcttgt acatagctca aaaatatttc
    25561 gattggtgtt ctgtaattca atgattttct tggaatatga ttacgttgat tgctgacact
    25621 ggaaataaat gtctgattca cttttctaaa atccattgat ttcggcagtc cattacgacg
    25681 cagaatcccg ttagaatt
//
LOCUS       DAZKNH010000033        24384 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000033 DAZKNH010000000
VERSION     DAZKNH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.033"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..616
                     /locus_tag="ACG8MG_001275"
     CDS             <1..616
                     /locus_tag="ACG8MG_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0375331.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            613..1386
                     /locus_tag="ACG8MG_001276"
     CDS             613..1386
                     /locus_tag="ACG8MG_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0375332.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1383..1781
                     /locus_tag="ACG8MG_001277"
     CDS             1383..1781
                     /locus_tag="ACG8MG_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFQ0375333.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1966..2637
                     /locus_tag="ACG8MG_001278"
     CDS             1966..2637
                     /locus_tag="ACG8MG_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375334.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2696..3136
                     /locus_tag="ACG8MG_001279"
     CDS             2696..3136
                     /locus_tag="ACG8MG_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375335.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3148..3816
                     /locus_tag="ACG8MG_001280"
     CDS             3148..3816
                     /locus_tag="ACG8MG_001280"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0375336.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3880..4503
                     /locus_tag="ACG8MG_001281"
     CDS             3880..4503
                     /locus_tag="ACG8MG_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFQ0375337.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4559..4945
                     /locus_tag="ACG8MG_001282"
     CDS             4559..4945
                     /locus_tag="ACG8MG_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFQ0375338.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5158..6549
                     /locus_tag="ACG8MG_001283"
     CDS             5158..6549
                     /locus_tag="ACG8MG_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFQ0375339.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            7160..7528
                     /locus_tag="ACG8MG_001284"
     CDS             7160..7528
                     /locus_tag="ACG8MG_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFQ0375340.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            7708..8649
                     /locus_tag="ACG8MG_001285"
     CDS             7708..8649
                     /locus_tag="ACG8MG_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFQ0375341.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            8718..9449
                     /locus_tag="ACG8MG_001286"
     CDS             8718..9449
                     /locus_tag="ACG8MG_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0375342.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            9593..11773
                     /locus_tag="ACG8MG_001287"
     CDS             9593..11773
                     /locus_tag="ACG8MG_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFQ0375343.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            11793..12275
                     /locus_tag="ACG8MG_001288"
     CDS             11793..12275
                     /locus_tag="ACG8MG_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFQ0375344.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            12291..13052
                     /locus_tag="ACG8MG_001289"
     CDS             12291..13052
                     /locus_tag="ACG8MG_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0375345.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            13183..14517
                     /gene="murC"
                     /locus_tag="ACG8MG_001290"
     CDS             13183..14517
                     /gene="murC"
                     /locus_tag="ACG8MG_001290"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFQ0375346.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            14824..15309
                     /locus_tag="ACG8MG_001291"
     CDS             14824..15309
                     /locus_tag="ACG8MG_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFQ0375347.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            15302..15985
                     /locus_tag="ACG8MG_001292"
     CDS             15302..15985
                     /locus_tag="ACG8MG_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFQ0375348.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            16111..18756
                     /gene="polA"
                     /locus_tag="ACG8MG_001293"
     CDS             16111..18756
                     /gene="polA"
                     /locus_tag="ACG8MG_001293"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFQ0375349.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHANTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            18801..19637
                     /gene="mutM"
                     /locus_tag="ACG8MG_001294"
     CDS             18801..19637
                     /gene="mutM"
                     /locus_tag="ACG8MG_001294"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFQ0375350.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            19601..20230
                     /gene="coaE"
                     /locus_tag="ACG8MG_001295"
     CDS             19601..20230
                     /gene="coaE"
                     /locus_tag="ACG8MG_001295"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFQ0375351.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            20282..20614
                     /locus_tag="ACG8MG_001296"
     CDS             20282..20614
                     /locus_tag="ACG8MG_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0375352.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            20926..21417
                     /gene="nrdR"
                     /locus_tag="ACG8MG_001297"
     CDS             20926..21417
                     /gene="nrdR"
                     /locus_tag="ACG8MG_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFQ0375353.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            21440..22813
                     /locus_tag="ACG8MG_001298"
     CDS             21440..22813
                     /locus_tag="ACG8MG_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0375354.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            22813..23739
                     /gene="dnaI"
                     /locus_tag="ACG8MG_001299"
     CDS             22813..23739
                     /gene="dnaI"
                     /locus_tag="ACG8MG_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFQ0375355.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            23868..24308
                     /locus_tag="ACG8MG_001300"
     CDS             23868..24308
                     /locus_tag="ACG8MG_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFQ0375356.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         8160 a         4172 c         5373 g         6679 t
ORIGIN      
        1 gtttgttttt gcaccgttag aaggagaaga cctcgcttca tttctggttg gtgcggcaat
       61 catgatgatc ggaatgacgc tctttttgtt tggagcagat tattctatga tggaagtcgg
      121 agatctagta gggaaatata tgatcaaaaa gaaaagtttg accgttttgg tcagtttggg
      181 atttgctatc gggatcgtga tcacgattgc tgaaccttcc gtgcaagttt tgggacaaca
      241 agtatatgat atatctgacg gagagattgg cagagtgttg ctgattggga tcgtcagcgt
      301 cggcactgga gtatttctag cctttgcgct gcttcgggtc gtattcaagc tatcctatta
      361 tcaattgatg gctataggct acataggcgt tttgatcgcc tcatttttca ccagttccga
      421 atttatgccg attgcttttg attcgggggg agtgactact ggtccagtaa ctgttccatt
      481 tatcttggcc cttgccggag ggatgactag tatgatcaaa caaaagaaaa atgaaaatga
      541 cagttttgga atggtcggta ttgcttcgtt agggccgatt cttgccgtga tgattttggg
      601 ggtgatcttc caatgagttt ctggcagcaa gtgatagatg gaatcgaaat cgttaccatg
      661 gaagtcattt tggcagtggc acctatcgta atcctatttg ttttaatgaa tatcattagt
      721 tttcgtatgc gcaaaaagcg tttctttgac attatgaaag gtttcttgat aacgactatt
      781 gggttgatcc ttttcctgca cggagtcaat atggcttatg taccggtagg acagcattta
      841 ggcagtgcta ttgcgggatt gccttacaat tatatactgg ttccccttgg ctttttaatg
      901 gggttcttag tagggtttgc tgagccggcg attcatgtaa tgatcaaaca ggttgaagaa
      961 ttgagcgagg ggagaatccg atcgaaaatc atgctgatgg taatttcaat tgggatcggc
     1021 gcagcagtag gattgtctat gtggcgtttg ctcgtcggat tctctcttta ttatttcttg
     1081 atcccaggat acgggctagc atttatttta ggacgaaaag tggacaagat ctttttagca
     1141 atggcttttg ataacggtgg agtagcaacg ggcccgatgt gttcgacatt tattttatca
     1201 atgagcgtgg caatcgctgc acaaattgaa ggaagagatc cgatcattga cgggtttggt
     1261 gtggttgcaa tgattgcatt gacaccaata ttatccactt tagtactagg atatatttat
     1321 aaacgaaaag atgaaaaaga cagaatcgaa aaagaaagat tgcaaaaatt gggggaaagc
     1381 aaatgaaaga tcttgtagca ttgaatcttc acatgattgt gacgatcgtt gacggaggaa
     1441 tgggcgaaga tgttatcgac ttttcaaaag aagcaggtgc agcaggcgga acgatcttac
     1501 atggaagagg ttctggtgta catgattcag ggaaattttt aggtttggac atttcgccag
     1561 aaaaagatat tgtgttgaca ttagtacccg atagtttgac gaataaagta atggatgcga
     1621 ttggtcaagg catcaacatc gatattccag gtaatggtat ttgttttagt attgatgtag
     1681 acaaagtcat tggtatcact aagattcatc aatatcgaga aattatcgga atgcaagaac
     1741 ttttagatga aaataacgat agagaaaagt ctgaatcata gacaagcgtt tgaagaggaa
     1801 ataaacacaa acaagaaggc ggatgagcgt tatttccgct tttttgtttg tatttttctc
     1861 gtgaatactt atgaaatagt aaatttttat gaaattctac tctaagcatc agactttctt
     1921 ttcaaaatat gctattttaa aaatgatgaa aaaaggggga gaaaaatgaa ttttttagtt
     1981 gttttaaaag atactctgat tgagcgattt acatttctat atgttcaatt gcaggaaaat
     2041 aaaacattgc tgcatttttc accagagttc catctagagg ggctaacatt aggagaaatc
     2101 tatcaagcag aagggctgtc ggctgttttg cactttttta aggtagaatt ggaattacca
     2161 gtaaaagatt atatcgagct ttctcttgaa tcttgggatc gagcagtggt tgaactgttt
     2221 ccagaaggtt taatgatcga tacaaacgat gggaagatcc atctgactgc agcagaactg
     2281 caaaaacaaa tgcgttatca aatagatgac gaaaattctt tttctatttt tagacgtcag
     2341 caaaagattc taaaaagctt tttaaaagtg atcagtaaaa aacggaatct gatcaaaacg
     2401 acacagctgt tgaaaaaata tccaggtctg cttcacacgt ctattcaatt tcctcaattg
     2461 attacgatga tccatcgttt tatacgtatg caacagctac catctaagaa gctgtttctt
     2521 ccattgacgg acacatttcg agtagtcaac agagaagata aaaagaaagt gattgtgatt
     2581 gactttacca gaaatcggaa tgtccttcac caagttgcaa tggaaaaggc caattaaggc
     2641 tttttctttt tggatttttt tgtcataata aatattagat gatggaggag ataatatgat
     2701 gcaaacatgg ttagaaaggc taatacgatt tcagcagaac tatcacaaac ttatgcaggg
     2761 aagatatgcg aaatttgatc agttgaatcg cggtctattg atcgctgcgc ttgtctttag
     2821 tttgctaagt cgttggttgc cctatagaat cggacaattg ttgttcatcg tctttttcgg
     2881 atggatgata tttcgtttcc tatcaaaaaa aatctacccc agactaaatg agaatcaacg
     2941 ttatttgaaa tatcttgata gagtcaaaga aaaatatcat cagataaaaa acaaaaagca
     3001 agtcaaaatg gatcgaaaaa gttatacttt ttttgagtgc ccgaattgtc atcaaaagca
     3061 gcgtgcacct aaaggaaaag gacgaatacg tgtgacatgc aaaacatgtg gtataaaatt
     3121 cgaaacgaat gtttaaggga gaaaagaatg aaattagtat tattacgtca tggcgaaagc
     3181 gaagcaaatt ttgaaaatta ctggacagga tggctagatg tcgccttaac agaaaaagga
     3241 aaagaacaag cagcagttgc cggtcaaaaa atgcttgaag ccggacttca attcgatgcc
     3301 gtttatacct ctgttttgaa acgtgcgatt cttaccggac aagtcgcact agaagaaatg
     3361 ggtcagttat ggcttcctat aataaaaaca tggcgtctaa atgaacggca ttatggggat
     3421 cttgtgggta aaaacaaaga taagatgaaa gaaatatacg gaaaagatca agtaaaaaca
     3481 tggcgcagag gatatacgga agttccgccg ttaaccgcag aaaatagatt tgaccgcaga
     3541 tatgatcact tggatccgca tttgatccca tacggagaaa gtctggaaat gacagtgaat
     3601 cggatcattc ccttatggca agatcatcta gcaccaaaac taaaagatcg gcaagattta
     3661 ctagttgtgg gtcatggtaa tagcatccga gcactgacaa aatatttaga agatgttcct
     3721 gaagatcaaa tggatacgat cgatatccca aatgcacaac caatccagta tacattagca
     3781 gatgatttga caattctgga caaaaaaata ctttaaagaa cacctttatc taaaagtgag
     3841 agatagaaaa aagaagcaaa ggaatggaac aaaaggaaca tgaaaaatta tatatgggac
     3901 tttgatggta cattgtttga tacgtatccc gcaatggtag atggtgcatg gcaagcacta
     3961 aaagattttg gtatcagtat ggacaaaaaa gaaatctact tcaaaatgaa aaagtattct
     4021 actagttatt tgatcaatga atcaaacttg aatgccagag agtttaatga attatttcac
     4081 cgatatgaaa aagaaagtac ggaagtttcc cgtccatttc cagaaacgaa acaagtactg
     4141 gaaatgctaa aagacaacgg cggtcgtcat ttcatcctga cccaccgttt gacagaatca
     4201 acatggggcc tgttaaaaga acatcgattg gcacatctga ttgaggaagt agtagggatc
     4261 gatcaggact ttcctcgtaa acctgatcca gcatctttaa attatttgat cgatactttt
     4321 catttagaaa gaacagacac gatgatgatc ggcgatcgtc gtttagatat cgaagcaggg
     4381 aaaaatgcag gagtcgctac ttgtctttac gacatcgatc attttctggg tgagatcccg
     4441 gcagattatg tggtaggaaa tctgaatgaa atccttacct tgcagagcat cattaaaaag
     4501 tagtatcccc tttcaaagga gatatgttat aattttcata aaggagtgat aaaattatat
     4561 ggaaatcaga agacaaaaag aagttgtaga agcatttcat tatgatatga gattgccgaa
     4621 ccaagaagta gaaacagatt tgaaagtagg cttctcgcct ttaaaagaaa cgatcgaaaa
     4681 ctatccgaaa gaacatagca tcgtaggagc acgcttagag ttccgtttag tgtttgatac
     4741 atatgtttta tctggaagtg tgagtcagat caatcatatc ctcaatcgga aaattgaagg
     4801 tcaaaatgat gtcacacaag aagaagtgga cgagctagta gcaccgttat tcgatatcgt
     4861 acgccgaatg tcttatgagg tgacagagat cgccttagat cagcctgggg taaaattgaa
     4921 ttttcaatca gaaaaacaag aatagacagt aaaaacatac ctgcgataga ttaaagctat
     4981 cacaggtatg tttttttatt actaattgta agcaaaaaga atgtcatttc gataaaaatc
     5041 gtctaaaata taaaagtcac tgaaaaaaat tttttcagga ggatggttga agaatcattc
     5101 tcgggaacct ttacttcctt cgatggactt gtcattaata atatgaggag gagaaaaatg
     5161 acaacactca tcacagtttt agtggttcta gtttttgcgg cattattata tgtgttctat
     5221 cgtatgcaaa agcgccatac aaaattttca acacgcgtct ttacagcatt aggagttggt
     5281 atcgtctttg gcggtctgct tcaattgatc tttggtgtag acgacaaagt cgttactcaa
     5341 tcaatggaat ggatcgggat tgtaggtcaa ggatatgtat cattcttgca aatgctagtt
     5401 atgcctttag tgtttatttc gatcattggt gcattcacaa aaatgaaagt ttctgaaaag
     5461 ctagggaaaa ttagtgcgac tgtattgact tctttacttg gaacaacagc tattgcagca
     5521 ttgatcggaa ttggttcaac aatgctgttc ggactccaag gagctaaatt cacacaagga
     5581 gctgcagaaa cagcacgaat ctctgaactg gcaacacgtc aggaaacagt tgaaaactta
     5641 tcgattccac agcagatttt atcctttatc cctaaaaacg tttttgctga tttagcaggt
     5701 agtcgtgcaa caagtacgat tggagtcgtt attttcgcgg catttgtcgg aatcgcttat
     5761 ctaggcgtga aaagaaaagc gccaaaagaa gcagcattct tttcagattt agtcgacagt
     5821 ctgtataaaa tcattatgcg gatcgtgacg cttgttttgc gtttgacacc ttatggagta
     5881 tttgctttga tgacaaacgt tttggcaaca agtgattttg cagctatcgt caatttaggt
     5941 aaatttgtcg ttgcttctta cgtagcattg cttgctgttt tgattgtcca ctcactgatt
     6001 ttgattggtg caaaagtcaa tccaatcaac tatttcaaaa aagcctttcc ggtattgagc
     6061 tttgcgttta cttcacgttc aagtgctgga gctttgccat tgaatatcga gactcagaca
     6121 aaagctttag gagtagatga tgcaacagct aatttctcag caagttttgg tttgtctatt
     6181 ggacaaaacg gctgtgctgg cgtttatcca gctatgttgg caacgatcgt cgcaccagca
     6241 gctggagtga atgtctttag tttggaattt attttgatgc ttgtggcaat tgtaacaatc
     6301 agttcttttg gtgtagctgg agtcggtggg ggcgcaacat ttgcttcact gatcgtttta
     6361 ggggcaatga acttgccagt agcaatcgtt gggttagtta tctctgttga accattaatc
     6421 gatatggcac gtacagcagt aaacgtcagc ggtagcatgg tagctggtat cgtgacaagt
     6481 gcacgcatca aagatttaga tcgcgatgtc atcaatgatg attctaaagt gatcgatgtg
     6541 aatgcatagt caaaaagagg ttgtgataga agtggtcagc tttaagaaat aagaagaagt
     6601 ttcctaaaaa tagtttctca aatttttgtg aaatttcggc ttatttccga gaagctactt
     6661 ctgaaacacc gtgaataaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
     6721 ccaaacaaaa atagttcctc atattttacg tttggttgag cttattaccg cagatgcaag
     6781 cttttgaaca ccgtttaaga aagaggtcgt gagcctgccg tttcactctg agtcacaaag
     6841 agcaatttca aaaaatagct tctcatgttt gttgaatatt gtgtcttgtg accgaggagt
     6901 tgtcaggaga acaccgttta ccagaggttg tgacttgttt ttagtcacga cctctttttt
     6961 tatttattcc atataaatac acattattta ttccgaaaag tgaaagcgca aataacaaaa
     7021 acaaccaact tatttattat taaagaaaag cgtatttaag taggacgatt ctgttgagag
     7081 ctttcgtaga ttttcccatt ttcctatggt aaactgacaa agttgagaat gttagaacag
     7141 atcgtttagg aggaaacaga tgtttacgaa tgaaataaaa atcatgcttt atgtggacga
     7201 tgtagaaaaa aatgcaacgt tttggagagc agttggcttt gcggaaaaag atcgtcaaga
     7261 aatggatggg acgcttataa ttgaagtagg agttgaggac agtcaaacct catttctgtt
     7321 atatgatcga gcatttgttg agtcccattc acctggcaca gctactagtg ccccatcttt
     7381 gatgttttac agtaaagata tctttggact atataaaaaa atgcagacgc aatcagtcac
     7441 attaggtgat atggtacagc ttggagaaga atatgtcttt aactttgctg atcccgatgg
     7501 caactacttt gcggttacag gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa
     7561 caggaaagcg aagtcttttt aattcaaata ataatattat gtaaacaaaa aactttccat
     7621 gcgtaattat acacaagcaa ataaatcgtc aaatgcgaac gccttcttta tactgtttag
     7681 gaacgaaaag aaggaaggaa gaaatacatg gattatcaag tactgcttta ttacaattat
     7741 acaaaaatcg aagaaccaga ggaatttgcc aaaaatcatt tagccttttg caaatctttg
     7801 gatcttaaag gtcgtattct agtagctgaa gaaggaataa atggtactgt gtctggaacc
     7861 gttgaaaaca cgactgccta tatgaaagca atgaaagctg atcctcgatt tgctgatacg
     7921 atgttcaaga tcgatccaag cgaaggacat gcctttaaga agatgtttgt aagataccgt
     7981 ccagagcttg taagtttaaa gctagaagag gacattgatc caaaccaagt gacaggtgcc
     8041 tatctttcac cgcaagaatt taaagaagct attttagatg aacagacagt ggtgattgat
     8101 gcacggaatg attatgaata tgacttggga cattttcgag gggctgtacg cccagatata
     8161 cgcagttttc gagagcttcc agactggatc cgcgagcaca aagaagaatt tatggaaaaa
     8221 cgggtagtga cctactgtac tggtggcatt cgttgcgaga agttttctgg ttggcttgtc
     8281 cgtgaaggat tcaaagatgt cggacagctg catggcggta ttgctactta tggtaaggat
     8341 cctgaagtcc aaggagaatt atgggacggc aagatgtatg tgtttgatga acggatcgct
     8401 gtcgatatca accaagtaga taaacaaatc gtcggtcgcg actggtttga cggaacacca
     8461 tgtgaacgat atatcaattg tagcaatcct gagtgcaatc gtcagattct tgcttccatt
     8521 gaaaatgaag aaaaacattt aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat
     8581 cgctatgttc aagaccatgg aatctctcca gaagaagcag aactgcggtt tacagcttta
     8641 attaaataga aaacaatagt tttgattgtc gaccatttcc aatatgcgct atactagcgt
     8701 tgaggatggt gatccacatg aaatcattga tttatgctat tggggatgtc catggcaaac
     8761 atgagctttt tgaaaaactt attcactatt atgatccaaa gatccatcag cttgttttat
     8821 taggggatct caatgatcga ggtcctcgta caaaagaatg ttttttatta ggaaagcaat
     8881 tagtcgaaga aacaggagcc atttacctcc ggggaaacca tgaagaatat tttttgcaat
     8941 ttctgcaatc accggaggac tggtatgcgc cttatatgag aaatggtgga aaagaaacgt
     9001 ttgaaagcct gattcaccca ggggcatcgg aggaatactc cccaactgaa atctcgatga
     9061 tgatccaatc gagatattca gaactgatta aatttttagt ccagcgcccc ttgtattatg
     9121 aatgggaaaa ttatcttttt gtccatgccg gtgtggattt gacaaagaaa gactggcgta
     9181 agacaagtct aagagacttt atttggataa gggaaccatt tcatgaaggg aaaaacaata
     9241 caggtaagac aattgtattt ggacatacga ttacaccaat gctccatgga gatatgcaga
     9301 caacagacct ctggattcag gatcataaaa tcggaatgga cggtggggct gtattcggag
     9361 ggtcattgca tggggttgtt tttgacaagg aaggtattct tcaagatatt gaatatcaga
     9421 acaacgaagg accttggcat ggaatctgac taatggaaag aatgaaggag ctgtgacttt
     9481 gttgcggctc tttttatttt tatagagaat attgtttctt tcagtagttt aacccaggat
     9541 aaaaagtatg atagaataaa gaagcaattt ttagcagaaa agaggcattt taatgacgga
     9601 aaatttgaac cagacgatca ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa
     9661 gcagttgact actgtactta atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg
     9721 ttaccgaaaa gaaatgactg gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg
     9781 gtatgcctat cttgaaaact tagaaaaacg taaaactgaa gtgattcggc taatcgatga
     9841 acaagggaaa ctgactcccg agttagaagc agaaatcacc caagccgtga aaatgcaaca
     9901 agtggaagat ttgtatcgtc catacaaaca aaaacgacgg acaaaagcaa cgattgctaa
     9961 agaaaaagga cttgaaccac tcgcgaagtg gctgatgcag cttacagatg gagaggtgca
    10021 atctgaagca gaaaaatata ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt
    10081 gcacggtgct catgagatta ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg
    10141 gattcggtct tatacgtata acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc
    10201 agatgaaaaa ggcgtatatg aaatgtatta tgactttgca gaacccgtcc ataaaatggt
    10261 ttctcatcga atattagcta ctaatcgtgg agaaaaagaa gatattctga aagtattctt
    10321 tcaagtagat gaagcagcaa ttttagctta cttggatcgc caaatagtca aaaatcctgc
    10381 ctctccttct tcttcattcg tcagagaagc ctatcaggat agttataaac gctttattca
    10441 gccagcaatc gagcgagaac tgcgcaatga attgactgaa aaggcagatg agcaagcaat
    10501 cgctattttc ggcgaaaacc tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt
    10561 tgtcctggga tttgatccgg cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac
    10621 agggaaagtt ttagcaatcg aagttattta tcctcataaa cctgcagcgc aagcaaaaag
    10681 agaagctgct ggaccagctt ttatccaatt aattaataaa tatcaagtag atatggtagc
    10741 tatcggaaat ggtacagcca gtcgggaatc tgaacttttt gtggctgagc agttgaaatc
    10801 cgccgaccat aaaacctatt acgctatcgt caacgaagca ggcgcttctg tttactccgc
    10861 tagcgaaatt gcacgaaaag aatttccgca tctacaagtt gaagaaagaa gtgctgtttc
    10921 tattgcccgt cgcctgcaag atcctttagc tgaattagtc aaaatcgatc ctaaagctgt
    10981 cggagtaggc cagtaccaac atgacgtttc tcaaaaacgt ttggctgaac aattggattt
    11041 tgttgtggaa acagcggtta accaagtagg tgtggatgtg aatacagcaa gcccgcagct
    11101 cttgcagcat atatcaggat tgaataaaac aactgcccaa aacattgtca tttatcgtga
    11161 agaaaacgga gaattcaccg cacgaactca attgaaaaaa gtgccacgct taggaccaaa
    11221 agcatatgaa caagcaatcg gattcttgcg agtccctggt gggaaaaaca ttttggacaa
    11281 cacaggtatc catcccgaaa gttattctat tgcaaaagaa atattaactt cagtccaact
    11341 ttcagaaaaa gagcttggta cagaagaagc agtagaaaaa ttgacacgat tatccgtaga
    11401 gaaactggct gaaagcctat cagtcggaga agaaacgtta gccgatattt tagctgggct
    11461 gacacaacct ggacgagata tgcgtgatga gatgccagcg cccttattac gtacagatgt
    11521 attgagtatg gaagacttga aaccagggat ggaattgact ggaacggtcc gtaatgtcat
    11581 tgacttcggc gcatttgttg atatcggtgt gaagcaagac ggacttgtcc atatatcgaa
    11641 actcagcaaa aaattcgtca agcatcctac agatgtcgtg tcagtaggag atatcgtaac
    11701 agtttggata gaacaagtcg atacgaaaaa aggcagaatc agtttgacga tgctttcccc
    11761 ttatgaagag taacaatgag gagtaacaaa aattgacaca agaagaactg cagcagttag
    11821 tagaagagat atcattaaaa agcttccagc ttcctttcaa gcaccaagca atgttcaatc
    11881 cacgcttacg tacaactggc gggcgctatc atttaaacga tcatcattta gattttaatg
    11941 cgaagctatt tgaagagtca gacgaagaga cgattgcagg aatcatcaaa catgaacttt
    12001 gccattatca tcttcatcta gctggaagag ggtatcagca taaagatatg gatttcaagt
    12061 atcttttgaa aaaaacagga gggttgcgct atgcaccagc cttgacgaag aaacagataa
    12121 agatggagta ttatcgatgc caagattgtt ctgccacgat ctgccgcaag agaagaatcg
    12181 atgtacgcaa atatcgttgt ggaagatgtc atggagaaat caaatggatt gagacaaaaa
    12241 gagaagactt taatgaagca caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa
    12301 gtgagcagag gaatctaaaa gactggaatc agctattaaa ttttttagcc gcacctgaag
    12361 agggaaattt tgaggaactg acggcaccta cacttatttt agctggaaac tgtcttccta
    12421 tcttagctga taaagcagca caaatgtatt taaatggcca agtagatcaa ttgtttttgg
    12481 tcggaggggt ggggcatgcg actagaatcc tttatgagaa ttttgaaaag caaggatttc
    12541 attttgaaga tggcatgagt gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg
    12601 atttgcccga taaggctttt ctgatagaat cgaagtctac aaattctgga gagaacgcca
    12661 ctttttcact ggagattctt cattccttag gagctgtacc agaaaaaatc ttattgatga
    12721 atgatccgac actgcaacgc aggactcgag caacttttga gaaagtatgg caaaatgaac
    12781 aaactatttt cgcgaatgct gtgccatttg tgccggaaat ccttcacttt tctgaagaga
    12841 tcatctttac agcaaaagaa ctgaatcatc aatggcctaa ggaatatttc catgcgctag
    12901 tattaggaga aatggaacgt ctccatgacg atgagaacgg ctatggtccg aaaggaaaag
    12961 attttattcc tcacattgat atatcagagg aagtttggtc ttcttacacc agaattaaag
    13021 agtcaataaa aacggatttt tctcgtactt gaccatttta gcaagcgaat cctcctttcc
    13081 aaataaattt ggttgaaatt ttcaatcgtt tataagataa tgaggagcgt gttgaatgta
    13141 atagctaaaa gaatggacgc tagataagag aagaggattc aaatggaaaa tcaaaataaa
    13201 ttgtatcatt ttgttggtat caaaggttca ggaatgagct ccttggctct tgttttacat
    13261 gaacaaggtc taaatgtcca aggatcagac attgaaaaat atttctttac acaaagagat
    13321 ttagaaaaag caaatatcac gattctgcca tttaacgcag ataatgtaaa accagggatg
    13381 acgatcattg caggaaatgc atttcctgat tcacatgaag aaatccagcg agcaaaagaa
    13441 ttaggtttgg aagtgattcg ctatcacgac tttattggtc attttatcca aaattatacg
    13501 agtatcgctg taacaggctc tcacggaaaa acaagtacga ctggactgct ctctcatgta
    13561 ttgagtggtg tccgtccaac aagttatcta attggagatg gaacaggaca tggtgatccg
    13621 caagcggaat tcttttcatt tgaggcctgt gaatatcgcc gtcatttcct tgcctattca
    13681 ccagactatg caatcatgac caatatcgat tttgatcatc cggattatta tacaagtatc
    13741 gacgatgtat ttacagcttt ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg
    13801 tatggtgacg atgcttattt acgcaaatta aaagcaaatg tgccgattta ttattatggt
    13861 gtcacagaaa acgatgatat ccaagcacga aacatcgaac gcacaacttc tggttctgct
    13921 tttgatgtct atcatgggga tgaatttgtt gggcacttca ctgtaccagc attcggtaaa
    13981 cacaatatct taaatgcttt aggtgttatt gcagtagctt atttcgaaaa actggatctg
    14041 aaagaagttg cagaagaaat gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag
    14101 atcgtagcag acatgaccgt agtcgatgat tatgcgcatc accctgcaga gatcaaagca
    14161 acgattgatg gcgcacgcca aaaatatcca gataaagaaa taattgcagt tttccaaccc
    14221 catacgttta cgcgtacgat tgcattgatg gatgaatttg cagaagcact tgatttagct
    14281 gataaagttt acttatgcga tatttttggt tcggctcggg aagaacaagg aaacgttaag
    14341 attgaagacc ttggagcgaa aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta
    14401 tctccattgt tagattatca tgatgcagta gtaatcttca tgggtgctgg agatgttcaa
    14461 aaatttgaac aagcgtatga aaaactttta agcagcacga cgaaaaatgt actgtaatac
    14521 aattactgca gtgtccttat aaaaatagaa agagggggag tggcaaaaat cttgccaagc
    14581 cccctttttt gcaacttatg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc
    14641 caatcaaatc aaaaacatga gaagctgttt tttcttggct gttcttatta tttgatgcag
    14701 gacagctttt tcacgacctc tgtaaactat aggaatctgt taaatcaagg gagcagaact
    14761 tgccttttac gctacttttg gtacaataat gtatgattaa aaataaactg gagggtttct
    14821 atcttgaaca taggtaagcc gagaaaacta ggaaaaacaa taaatgaaat cgaggaaggc
    14881 gactcgttat ccttaacaga atctattgaa gataaccagc ttttgctgta tttgggattg
    14941 acgaatgatg caaatccttt gtatatccaa catgactttg cacaaaaaac agaatatcaa
    15001 cagccaatcg ttccatcagt gatgctgatg gggatcatca caagtgcaat ttcaaagcat
    15061 cttcctggac caggatcaca tgttgtcaat ttttctgtca attttattga accggttttc
    15121 cattatgaaa cactgacttt tcagtttgag gtcataaaag tagataagat gaaggatgtg
    15181 gtgacgattt ctgtagaagc ggagaatacg aacgaagatc gtgtgttgga tgctgtcgtt
    15241 atggtccaac cgccacaagt tcctgaattg gaaatagcaa tcgaaagtga aggtgaattg
    15301 aatgaataat ccaagtacat caatgtcagg tatcaatcgt ttttatgcaa aagtttatgg
    15361 atttttaggt atgggacttg cccttagtgc agtcacttct tacttggctc tttgggtatt
    15421 tcctttgcaa ttagcaactt ttatcaataa ttttccacta ggctttgtgg gtctgtggat
    15481 cgttgaactg attttagtaa tggttctagg cgtaaaagca caaaagaatc cgagcttagc
    15541 aatcggtgga tttattgcct actctctttt gaatggcata acattagcgg ttactcttgc
    15601 atactacgat attggaacag taacgcaagc tttcttgaca gctactttga tgttcatggg
    15661 gatgtctgtt gtcggagtaa caacaaaaag agatcttagc ggggttggaa gagcaggaca
    15721 ggcagcttta atcggagtga tcattgcact agtcttgaac atcttcattt tgagaagtag
    15781 cgtagttgag ctgttcatct ccctactgtt agtcgtcatc tttgcaggga ttacagctta
    15841 tgataaccaa agcatccgta atatctatgt ccagacaaac ggaacagcac atagtggaat
    15901 tgctgtattc atggcattga agctgtattt ggactttatc aacttgttct tggcgtttct
    15961 gagaatcttt ggacggaaca actaaaaaag aaaaaaattc atcagttggg gctataccga
    16021 attatttggt atggtcccgc tttttctttt cgcattccgc aggattcttt gttagaatga
    16081 ataagaactt tacagtaagg agttaagaag atgacaaaaa ataaattatt actcgttgat
    16141 gggaacagtg tggcttttcg cgcattcttt gcgcttcaca attctcttga acgtttcaaa
    16201 aatagaaacg gtcttcatac aaatgcaatc tatgcattca atacgatgtt tgaaaatgtt
    16261 atgcaaaaag agcaaccgac tcatgttctg gttgcgttcg atgcaggaaa aacgactttt
    16321 cggacagaaa tgtatgcgga gtacaaaggt ggaagatcca aaacacctgg cgaattcaaa
    16381 gaacaaatgc catatatacg tgatttgttg acaggactag gggtacagta ttacgaattg
    16441 cccaattatg aagcagatga tattatcggt actttggcag aaaaagtaga caaagatcaa
    16501 tttgatgtcg tcgtgctttc aggtgatcgg gatttgactc aattagctag taaagaagta
    16561 aaagtggata tcactgtaaa aggtgtcagc gatatcgaaa gctatacacc agaacatgta
    16621 gcggaaaaat atgatgggtt gacgccaaaa cagattattg acatgaaggg actagccgga
    16681 gatgcctctg acaatatccc cggtgtgact aaaatcggtg aaaaaacagc tatcaagcta
    16741 ttgaaacaat atggctcagt cgaaggaatt tatgaacata tcgatgaaat gaagcaaagc
    16801 aaaatgaaag aaaatctgat aaatgacaaa gagcaggctt tcttatcgaa aaaattggcg
    16861 accatcaata cttcagcgcc tgtggatgtc tctatcgact cattgaagta tgaagggaaa
    16921 aatctcgata aactcgttcc gttttataaa gaaatggatt tcaatcagtt cctttctaaa
    16981 ttgaatatcg tagaagaaga agtaaaaatg gatgatattt tgtttgaagt cgttcatgaa
    17041 ttcaaagagg aaatgtttac tacggatatg gcgttgtacg tggaaatgat gggcgacaat
    17101 tatcatacag aggaaatcgt cggagtagcg tgggggacag aaaagaaaat ttatgtcacg
    17161 aacgatctat ctgtattcga aaaccgagcg ttccattctt ggatcactga tccgactcgg
    17221 ctgaaaaaag tatatgatgc gaaacgaact tatgttgctt taaaccgcta tactggaaaa
    17281 acgaaaggaa tcgattttga tgtcttgcta ggtgcatacc ttctcgatac gaatgaaaaa
    17341 agcacagata tcgaaggaat agctgctcat tacggataca acgatatcca atcagatgaa
    17401 gctgtatacg gcaaaggggc gaaaaaaggt ttaccagaag aagaggaact cttttttgct
    17461 catttagcgc gaaaagtagc tgcaatcaat gcgttgacac caaaattgag ccaagaactg
    17521 gcagaaaaaa accaagaaga tcttttccgg aaaatggaac tgccattgtc tatcattttg
    17581 gcagagatgg aaataagcgg gatcaaagta gatgcgactc gcttacaaga aatgaagggc
    17641 gagttctctg ctcgcttaag agagatcgaa caaaaaatct acgaagaagc cggcgaagaa
    17701 ttcaatttga attcaccaaa gcaattagga gtgatcttat ttgaaaaaat gggattaccc
    17761 gttatcaaaa aaacaaaaac tggatattcc acagccgtag atgtactgga acagcttcgt
    17821 gaacaagcac caatcgtaga ggatatcctc acctatcgtc aaattgcaaa aattcaatct
    17881 acctatgtcg agggactgct gaaagtgatc caatcagatg gaaaagtgca tacccgttat
    17941 gtccagacat tgacccaaac aggacgatta agctctgttg atcctaattt gcagaacatc
    18001 ccgattcgat tagatgaagg gagaaagata cgtcaggcat ttgttcctag agaaaaagac
    18061 tggctgattt attcttctga ctattcacaa atcgagttac gcgtacttgc gcatatctca
    18121 aatgatgaac atttgaaagc tgccttccta gaaggtcaag atatccatgc caacacagcg
    18181 atgcgtgtat ttggaatcga aaaggcagag gatgtcacac caaatatgcg taggcaggca
    18241 aaagcagtga attttggtat cgtgtatggc atctctgatt acggactttc acaaaatcta
    18301 ggaatcagcc gaaaagcagc acagcaatac atcgatacgt actttgaaaa atacccaggt
    18361 gtcaaagaat atatggaaag aatcgtacgc gaagcaaaag accaaggcta cgtcgaaacg
    18421 ctgtatcacc gccgtcgata tctgccagac atcaattcaa gaaattacaa tatccgttct
    18481 tttgcggaac gaacagctat caatacacca atccaaggaa gtgctgctga tattttgaaa
    18541 atcgcgatga tcgaattgga taaacgatta aaagaaacag gtcttcaagc tacgatgctg
    18601 ttacaagttc atgatgaact tgtcttcgag gtaccggaaa aagaacttga atcattagat
    18661 aaattggtga aggaagttat ggaacaagct gtttctcttc atgtaccact gatcactgac
    18721 agcagttggg gaaaaacgtg gtacgaagca aaataaacat tgtttaagac agagcaatat
    18781 aatgaaggtg aaaagaaaaa atgccagaat taccagaagt agaaaccgtt cgtaaaggat
    18841 tggaacggtt agtagtagga aaaacgattc aaaaagttca ggtcttgtgg cccaagatca
    18901 tcgagcaacc agagacaccc atctttgagg cttctcttgt tggcgaaacc atccaatcca
    18961 ttggacgtag agggaagttt ttaatttttc atctcgatca ttgtgaactg atctcacact
    19021 tgcgaatgga aggaaaatat caatttacga aagaaaatac accaatcgat aaacatacgc
    19081 atgtactttt cttttttgag gatggaagcc aacttcgcta caacgatgta cgaaaatttg
    19141 gtcgtatgac aatcgtcgaa aaaggagcaa gtgcaactta ccgaggaatc atgaaattgg
    19201 gacctgagcc tttgccagat agcttcttat tagcagattt cgcaaatgga ctgaaaaagt
    19261 cccacaaagc aatcaagcca ttgctgctag accaacgtct agtgactggt ctaggtaata
    19321 tttatgtaga tgaagcatta tgggaagcga agatccatcc agaacaaccg gcgaacacac
    19381 tgcgccccaa agaggtagaa caattacgtc tatcgatcat tgatgttttg gatcgtgcaa
    19441 tagaagccgg aggtaccacg attcgcagtt atttgaatgc attaggagaa tcaggcggat
    19501 ttcaagtagc actgcatgtc tatcagcaaa ctggcaaacc atgtatacgt tgtggaacgc
    19561 cgatcgtgaa aacaaaagtg gcacaaaggg ggacgcacta ttgtccgaat tgtcaaaggc
    19621 taaaaatcgc aaagtaggat ttgttttagg tttgacgggt ggaatagcta ccggtaaaag
    19681 tacggcagcc aaagtctttc agtctcatgg atttcctttg atcgatggcg atgtgattgc
    19741 cagagaaact gttgagcctg gaacgcttgc tctgaggaaa attgtatcag tgtttggcca
    19801 agaggtattg cagcctgatg ggcaactaga tcgagggaaa cttgggatga ttgtttttcc
    19861 atcaaaggaa aaacggcaaa aacttgatca gctattagat ccgtttatcc gaaaagcgat
    19921 aaaagatcaa atcgcctctc taagttccaa tcatccttta gtaattgtag atatcccatt
    19981 gctctacgaa ggtcattatg accactatat ggatgcagta gctgtcgttt atactacgcc
    20041 agaaactcag cttcagcgtt tgatgaaacg taatcaactg acgctagaac aagcgcagca
    20101 gagaattagt agtcaacttc caatcgaaga aaaaaagcag cgtgcggata ttttgttcga
    20161 caacaatgga acaaaagaaa aacttgtggg acaaatcgaa aattggcttt ttgctaatcg
    20221 ctttctttga gtatctgtgt caattttgct atcctgagta tatagaaagt gagggatagc
    20281 gatgggatca ttaaaaaatc aaaaggataa agtcgtaggt aacgcaaaag aaaaaatcgg
    20341 cgagatgctc aataaagaaa atctaatcga tgaaggtcgt acgcaatcag aaacagcaga
    20401 agaaaaagaa aagcagtacc aagcaagaat cgatgaaata gaagaccagc aggcagaaga
    20461 agaagcgaag cggcaagatt tgactcaaga acgaaaaaat ccgacacctg atcgtagtaa
    20521 aacaccgctc ccagaaagtg accacattcc cgatgagcaa aaaatcgagg aacagcgaat
    20581 gaaacactat acaggaatcg aagagaagtt ataaaatagt aaaaaatcga gatgtgactt
    20641 gaagcagatg agaaggtgtt catctgcttc aagttttttg tgctagatga ctaaatcagc
    20701 ttttgtgtaa gttatttctc agtaatcaag gtgatcagta ttggaaaatt gaaatttagt
    20761 aaggacaggg gacacaatta cgcatgaaag tagaaaaaat tgttcttata tccgtgtttt
    20821 ttgggtagga cagtcgcgtt tgtgatttgt ttcgtgttat aatacaacta gttgtaaaga
    20881 aagacacatc ctttatataa aatgcgatgc aagaggtgag acaaaatgag atgtcccaga
    20941 tgtcaacata ataattcacg agtaatcgac agccgtcaag cagatgatgg acgtgcgatc
    21001 cgtcgccgtc gcgaatgtga gaattgttcg ttccgattca ctacatttga acgaattgaa
    21061 gctgctccgt tattagtaat caagaaaaac ggggaacgcg aagagttcaa tcgcgataaa
    21121 attttaagag gactcatccg ttcagcagaa aaaagacctg tagctatgga acaaatggaa
    21181 caaatcgtca accatgtcga aaaccgcgta cgcagtctcg gagaaaacga agtatcgacg
    21241 aatctaattg gtgaatatgt gatggaagat ttagttgatt tagacgaaat cgcttatatt
    21301 cgttttgcaa gtgtttatcg ccagttcaaa gatatgtctg ttttcttaaa agagttgcaa
    21361 gatatcgtgg ataaagcaaa agaaaataat aaagagaata ataaagaaaa caattaagaa
    21421 acaaaaagga ggcatttcct tggaaaatgc ttggaaagag cttcaaccaa aaaatattta
    21481 ccaaggaaga aaagcggctc cgctgagaga agacagcaag aacgccttgc tgtatcttta
    21541 tcagccacta atcggcggag aagccttgag tatctatttc accctgttat cagaaatctc
    21601 tcaagaatct ggcaaaggtc ctgaagggat gcatgcagat ctgcttagta gtctaggctg
    21661 cggtatcccg caattttatg atgcaagaaa aaagctagaa ggaatcggct tactggatgt
    21721 ttattttaaa gaagatagtg atctgggacc tcgttttatt tatgagctgt tagaaccact
    21781 cattcctaaa gcatttttca aagactctgt tctggctttt cttcttctgg aaaaagttgg
    21841 tgaacgacga ttccagaagc ttacccaatt ctttgaacct aaaaggcata cagcagaggg
    21901 atatcaaaaa atcacaaaaa aatttttaga tgtttatcgc ttcaatgaag cagtgtacgc
    21961 tgctaatcaa gaaaacatcg aatcgctgaa taaatctttt gaagaatctg aatcagtagc
    22021 attacttgaa gcagattcga cattcgattg gcagtttttg aatgattggc tggcaaaaaa
    22081 acatattgca agtcttgatg atccaatcat caaacaagtc gacatgtatc atcagcttta
    22141 cggtttcgat gaattgacac ttggcgaact gatcagtcaa tcttatgact ttagtgaaca
    22201 aaaagtatct gaaaaagaac tgcaaaaagc cattttggca atgcagagac gtccggtgaa
    22261 gcaaacaaaa gaaacagttg cggtttctga aaacagtaat gaaccctcgt caaacgaaca
    22321 tttgacaaac gcagcgcttc agctgataaa agaagcacag agcattccac cgatgaaata
    22381 tctggaagca atcaagtcag aaaaaggcgg atatgtttct aaacaagaaa actggttact
    22441 gcaagatctt gtttctcaat caggtctttc cagtagcgtg atcaatgttt tgctgaatta
    22501 tgtactagtg atcaagaata atgctagctt aaatgccagt tatgtaaata cagtggcaaa
    22561 tgaatgggca caaaagaaaa tcgccacagc agaagaagcg atccagcaca ttcggcaaat
    22621 aagtaatcaa gcaaaacaat caaaacaaaa gaagcaaaca aattatcaaa ctggcaagcg
    22681 aaatgttcga cgggaaaagc ttcctgattg ggtcaaccag ccaaaagatg aaaaacagat
    22741 cagcagcgag aaaaaagcag agattgaccg ccgttttaaa gaatacctgg caaaaaagga
    22801 gggtgatatc tagtggaaga cgtaggcaaa gaattgaaga ggatcatcag caaacgcaat
    22861 tatcaagaaa agtatgctga aatgatcgct gaagtcttaa atgatgcgga tgttcaagct
    22921 tttctaagcg cacaccaaga agaattgaca caagcggata ttgaaaaaag ttacgctaaa
    22981 ttgtatgaat tcgtgcaaga aaagcgtaaa tatcaaaaaa acgatccaac gatgatcgct
    23041 cccggctatg agccgaagct gacgttgaat tttcattatg tggatgtcac ttatgtacca
    23101 acggaagcat tgttagcacg gcaaagacaa gaagaaatca gaagccgagt aaaagcaatg
    23161 gatatgccaa aagatattca atcggcttct tttgaaaact ttgaacgtac agatggacga
    23221 ggatatgcga gcttagaagc cctagatttt gtagagaaat atactgctga tccaaaaggg
    23281 tatcataaag gactgtatct agtaggaagt tttggtattg gaaaaacata tttattagga
    23341 gcaacagcga gagaattagc gattgctgga ttcaccacga cgctagtcca ttttccgaca
    23401 tttgccgttg agatgaaaca ggcaatcgga aaagatcaag tggcagaaaa gttagatgca
    23461 gtgaaacgtt cacctatttt gatgatcgat gacattggtg cagatgcgat gagtagttgg
    23521 atacgagacg aagtattcgg tgttatcctt caatatcgga tgcaggaaca gttaccaacc
    23581 ttcttctcat cgaattttac aatggatgaa ttggaacagc atttgtccgt cactcaacgt
    23641 ggagatgaag agccgctaaa agcgaaacgg ataatggaac gcatacgtta tttgacaaaa
    23701 gaaatcgaaa tgactggtcg caaccgacgc aatggttgaa cagttgacaa gtccaactga
    23761 catttgacct agtagtaaaa gcaggtcagc gaatcattat atggacagaa atgaccggag
    23821 acaatcaccg caacaagtaa tgcagaaact taaacggagg attttttatg agggctattt
    23881 tttctagtat atctgtagtt ggtctatttt ttcttttagc aggctgcggt agcgcagata
    23941 gcaacacagg ttctgttgat tcgattgatg cgggtcaaac agtaaaaaca agcagtacga
    24001 aaacggagag acaaagcata tttgtcggaa cgattgaaag tatcactcaa gtagaagcag
    24061 aagaagaaac gcttcagttg aaactcgtcg atgtcatggc caaagaagat ccagcgaata
    24121 tgggaacgtc attcagcaat gacggagtga tcttaaatgc tactgctgaa aaaataagcg
    24181 tggacataaa tgaattgaaa atcggtgaca aagtccaatt tacattagtt gagatgccga
    24241 tcatgacgat gtctatccca ccgcaagttc ctggagattc gattattgat gtttcattga
    24301 acaaataacc aataacattt tttcaaaaat aagaaaatgt tgttttatag aacgaaaagg
    24361 tatatttttt aaggagatat ggga
//
LOCUS       DAZKNH010000034        24338 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000034 DAZKNH010000000
VERSION     DAZKNH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.034"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..2478)
                     /locus_tag="ACG8MG_001301"
     CDS             complement(133..2478)
                     /locus_tag="ACG8MG_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFQ0375357.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2757..3659
                     /locus_tag="ACG8MG_001302"
     CDS             2757..3659
                     /locus_tag="ACG8MG_001302"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFQ0375358.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3870..5645
                     /locus_tag="ACG8MG_001303"
     CDS             3870..5645
                     /locus_tag="ACG8MG_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFQ0375359.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5711..7297)
                     /locus_tag="ACG8MG_001304"
     CDS             complement(5711..7297)
                     /locus_tag="ACG8MG_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFQ0375360.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7548..7772
                     /locus_tag="ACG8MG_001305"
     CDS             7548..7772
                     /locus_tag="ACG8MG_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFQ0375361.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7914..9665
                     /locus_tag="ACG8MG_001306"
     CDS             7914..9665
                     /locus_tag="ACG8MG_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFQ0375362.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9665..11449
                     /locus_tag="ACG8MG_001307"
     CDS             9665..11449
                     /locus_tag="ACG8MG_001307"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375363.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11784..12636
                     /gene="licT"
                     /locus_tag="ACG8MG_001308"
                     /pseudo
     CDS             11784..12636
                     /gene="licT"
                     /locus_tag="ACG8MG_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            12733..14619
                     /locus_tag="ACG8MG_001309"
     CDS             12733..14619
                     /locus_tag="ACG8MG_001309"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0375364.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14660..15562)
                     /locus_tag="ACG8MG_001310"
     CDS             complement(14660..15562)
                     /locus_tag="ACG8MG_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0375365.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15792..17195
                     /locus_tag="ACG8MG_001311"
     CDS             15792..17195
                     /locus_tag="ACG8MG_001311"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0375366.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17195..18628
                     /locus_tag="ACG8MG_001312"
     CDS             17195..18628
                     /locus_tag="ACG8MG_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0375367.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18773..19699
                     /gene="trxB"
                     /locus_tag="ACG8MG_001313"
     CDS             18773..19699
                     /gene="trxB"
                     /locus_tag="ACG8MG_001313"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFQ0375368.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19912..21639
                     /locus_tag="ACG8MG_001314"
     CDS             19912..21639
                     /locus_tag="ACG8MG_001314"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFQ0375369.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21655..22005
                     /locus_tag="ACG8MG_001315"
     CDS             21655..22005
                     /locus_tag="ACG8MG_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0375370.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22099..22959)
                     /locus_tag="ACG8MG_001316"
     CDS             complement(22099..22959)
                     /locus_tag="ACG8MG_001316"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFQ0375371.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23051..23479
                     /locus_tag="ACG8MG_001317"
     CDS             23051..23479
                     /locus_tag="ACG8MG_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0375372.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23641..23817
                     /gene="rpsU"
                     /locus_tag="ACG8MG_001318"
     CDS             23641..23817
                     /gene="rpsU"
                     /locus_tag="ACG8MG_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFQ0375373.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23844..24290
                     /locus_tag="ACG8MG_001319"
     CDS             23844..24290
                     /locus_tag="ACG8MG_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFQ0375374.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7708 a         4353 c         5187 g         7090 t
ORIGIN      
        1 ggtttttcac atttttacat aaatttgtct taatatcgta gatgtaagct tttgaacacc
       61 atttattcgg aaaaagggag ctgtgacaag atttttgtcg cagctccctt taattctgat
      121 taatctatta gatcaatcat ctttatcgtc tgattctttg gtctctggat tcgctctagc
      181 ataagctgaa gcggtgttcc agtaatctgt atagttttca tcagtaccgc ctacgccgaa
      241 tttaaaggcg cttttcttcg gaccattttt tgcccaaagt gactccactg tttttcctgg
      301 cgtttggatg gattttttat tgacagtaac ttttccagct gtttgtcctg tgaacccaga
      361 tacttttttc ttcatcacat cggatgataa gtcaaatttc tcatcagtgc cgaaaatctc
      421 agggtttgct tggtaagctt gattgattag attggcaaga taattagccg aacgtattct
      481 tgcttggctg tccattccag tattgtcatc gtgtcctgcc caactactga tagtgataga
      541 tggagttgag acgatcagcc atgaatctcg gtattcattg gtagaaccag tttttccgac
      601 ccaatctgca tttcctagat ttccgtttaa gcttgagatg acatctttga atggtgtagt
      661 gattttttca ttgatcacac tacgcatcat gtcattcatg atcgatgccg ttgcttcgga
      721 ataaatacgg acagaattac tttcatgttt ataaatcaca ttacctgcat tatctgtgat
      781 ggaatcaatg atatagcctt cttcgtagac tccaccattt gctaaggcct ggaatccatt
      841 tgtctgttga agcgtcgtta catctactgt ccctaaagga gccgattcaa cgccccaatt
      901 atcatttgct gggtagttca tcttcgccaa gtagtgctcg taagcgtatt gtttcgatcc
      961 accggaattc aacacatctt gatataagtg ataagctggg atattgttgg accattccag
     1021 tgactcacgg acggtttgga aggtgttcga cccttcattg gtcgcattga cgattttctc
     1081 accagcattt tttccttctt gccaagtcgt tgcataatct gatacacggg attctgatcc
     1141 gacaagtccc atatcgattg caggaccgta aacaagaacc ggtttgatgg atgaaccagc
     1201 ttgacgttct gtatcaaagg cgtggttatt ttgattctgg ctgtaattcc gaccacccac
     1261 gaagccatag atacgtccgg ttctgttatc catcaataca tttccagtct ccacttggct
     1321 accagcacca tcatctaaca tgtagccata attttctacg ccggtttgta atgcagcata
     1381 gatatcttta tcgatcgtcg aatggatcgt atatcctttg ttttggatcg tttgctgtgc
     1441 tttttgataa taagcttcac tgaccgatga atctgacata tcagcattat ctttttctgc
     1501 caattgttgt gcaataatcc ttgttgcttc gttcatcacg gtataataca agaattctct
     1561 gtcagtctgt tccgcgattt gttgtgggag aaagtctttt gtaagatcat aggctttcgc
     1621 ttcttcgtat tgttctttcg taatttgtcc ttcacggtac atactgaata aaacaaaatc
     1681 ttttctggca agaccggcag aaagatcttc ttttagcgcg cctgtattcg tgtatggact
     1741 atacgtgata gggctttgag gcaaaccagc gatataagca gcttgtggca gagtcacatc
     1801 ctttgcatct acaccaaaga ttcctctagc agcttcctgg actccagcaa tattttgccc
     1861 cttattattc cgaccaaatg gtgagacatt caaatacgta gtaatgatct cattttttga
     1921 aaagtgtttt tctacttgtg cagaaagaac gatctcgttt gctttacgtt tgaatgttgt
     1981 ttcatcggtt aggatctgtt gtttgactaa ttgctgtgtc aaagtggatc ctccagacga
     2041 gccgatgcca gtcgcttctg aaactagtgc tcgtaaaaca gctttaggga cataaccttg
     2101 atgtttatcg aagtattcat cttctgtact gataatcgcg gttttcaata aaggcgaaat
     2161 cttatcagat gaaatagtcg tccgcatcag atctgtctgg atttttgata tctctgtatt
     2221 atctgcataa acaatctttg acgtttccgt aatgtttcct agttctgtct gcagttcttt
     2281 tttagtcggc aattgtgtgt cttcgacaag ataggcaaag tagccagcac ctattccgat
     2341 acctagagct gcgaacaaca aaagaagaac agtaataaaa acagtcaatg attgaaacac
     2401 tctaaaaata atcttaggaa caaaccattt ttcttttttt cttctattct gtttttttcg
     2461 agtatttttg cgtggcaaaa gggtcacctc aactaatggt tttatttcgc taattatagc
     2521 aaataatagg acaaaaaaac agacaaggct gtttttgaca ggaatttctt tttcgaatga
     2581 gataaaacgt gaggaaatga tcttttttat gaaagagttc ttaagctttt ctttagggaa
     2641 agtttcagtt actatggaga acagggcaaa gaaaaacaaa ccattttctt tgtacatcat
     2701 cttatcaata tacaggaatt gttcgaaaca attgcctttc aaggaggaat ccatccatgt
     2761 tattaggctc acatgtcagc atgagcggaa agaaaatgct gttaggcgcg gcagaagaag
     2821 cagccagtta cggatcttcg acctttatga tctatacagg cgctcctcaa aatactagaa
     2881 gaaaagctat cgaagagatg aatattgatg ccggaaaagc atttatggca gaaaacggac
     2941 taacaaatat cgtcgttcat gcgccttata tcattaattt gggaaatacg atcaaaccag
     3001 aaatgtttcc atttgctgta caatttctga gagaagagat tgtccgtgcg gaagctttag
     3061 gtgctactca aatcaccctt cacccaggcg ctcatgtagg agcaggggca gaagctggga
     3121 ttgctagaat tattgagggg ttgaatgaag ttttgacaaa agaacagacg gcacagatcg
     3181 ctttggagac gatggcaggg aaaggtacgg aaatcggtcg aacatttgag gaattagcgc
     3241 aaatcatcga tggagtaaaa ttgaatgaaa aattatccgt tacttttgat acatgtcata
     3301 cgaatgatgc cggatataat ataaaagaag atttttcagg tgtaatggaa gagtttgata
     3361 aggtcatcgg attagatcgg cttaaagtac tgcatatcaa tgattctaaa aatccacaag
     3421 gttctcataa agatcggcat gccaatatcg gttttggaac aattggattt gacacattga
     3481 acaaaatcgt ccatgatcca atgtttgaca acatttcgaa aatattagaa accccatatg
     3541 tcggtaaaga taaaaagaaa gcttacgcac catatggaaa agaaatttcc atgttcaaag
     3601 cgcaagcatt cgatcccgaa gtgttccctg agttattagc agaagaagtc acactttaag
     3661 ttttctacct gacagcaaca aaaaaggatt gggcagtctg ccttgtcctt ttttgtttaa
     3721 gaaacaaagg agtgcttttt gcattcgttt tcgccatgct ccttttgccc cctcttttta
     3781 aattcagaca aacagggtat actgatagaa aagtaaaaaa cgaaaagttt cacaaacaag
     3841 tagcaaaacg tgattgaaag gacgaaaaga tgctgaacat tttaaacccc ttgatcgacc
     3901 aactaaaaaa gaaacataca tactttattc ttggcatagc cgtattattt acaattattc
     3961 ggttgtattt atatagtaaa gcaatctatg gaacagatat gtcggcgacc tatgatgatc
     4021 agctgctgat tcactatgct caaaatattt tggatggtaa atggctaggg gagtatacga
     4081 ctacaacact ttccaaagga atctcctaca gcttattttt agtacttgca cataaactgc
     4141 atctttctta tgcagttttg ttcggcatgt tcagtatttt tgccagtata ttggttgcac
     4201 tcgcgtttaa accgattgtg aagaacaagt atcttctttt aagtatctat ttttattttt
     4261 tgttttctcc gattagtttt acacatgaat attccactcg aacatatcgt aatgccctcg
     4321 tgattccggc tgttgagatc attatcgctt gtttattagc tatttattac cgcaaatacg
     4381 gttctagcag aaaattgctg ccatggtttt tagggctttc tgtcgctttt cctttctttt
     4441 ggttcattcg cgaagattct atctggcttc ttccttttac tgtagtagct ttgggtatca
     4501 gtttcgtcca gatcttgcta gggcaagact tttctttcaa agaagtaaag aagttaaaaa
     4561 aacgactctt tcttctctcg aaacgggaat ggctgaaact ttcattgttt ttacttccta
     4621 tagttggctt ttccatcgtc tatcagtgga tcgaatataa aaatgagaca acatacggta
     4681 ttgcaaccat caatgaccgt tccagtggag aatttggacg tttgatgaaa caactgatcc
     4741 gaatagatga tgaaacgaac ttgaataaag aaaacagtga gatatgggtc tcacatgctg
     4801 cactaaataa agcattagcc gcatctccta cattccatac tgtttctacc cgtattgacg
     4861 aattatatac gactcacgcg tggactcaag gtggaaaagt aaaagaatta cccggagata
     4921 tcatcttttg ggcattgcga gatgtggtaa gcgaatctgg ttattaccaa aacaatgcaa
     4981 aagaaaccaa tgatttttgg aaaaaagtca accaagaatt agaagaagcc tatcacaagg
     5041 gaaccatcaa aaagaaaaaa gagttctatc tgatgggatc aggagatgga aagacgatga
     5101 gtgatctgcc aatcgtattc gatttccttc aatcaggata cgcttatgca tggcgatata
     5161 aagatttcca tcaaggagga aattatagtt tcggaacgca agaacaagtc aaacaggctc
     5221 aagaattatt aaaccttccg ctaatggaga attggatcga tagtaatgaa ccgagaacac
     5281 ctaatttcca attaacaaaa tcagcaaaag tcgcgaatgt catcataaaa atttaccaag
     5341 ttctggctat tcccgtgctg attgcttcaa tagcagggat attacttatg atactaggaa
     5401 ccgtatttgc aaaagctgat cgtgcctatt atagttcctc tttgattgtg gtcaccggat
     5461 taatactgac acaattggtt tttcttttcg gtgtctcatg gttttgttct tatgcgccag
     5521 aaaaaaagga ctatttttta agtgtctaca ctggtgcagg cgtgccatta acgcaattca
     5581 tcgtagtaac aagcatgcta gttttcagta aattaaatct gaaaaaagct ttatcttcta
     5641 aataaaaaga cccttggtag ctagattttt aacctaacta tcaagggtct ttgtgtggaa
     5701 aaatcagttt ttattcgatt tcttctaccg tttcctgcaa aagctgttca tattttttgt
     5761 ttcctttata taaatcacca atcgtccatg ccaacaggag aaggacaaga acaatgatta
     5821 cataagcgat gtgatcagcc aatgctagtt gagcggaagt ggcttgctcg ccgaagaaac
     5881 tttcgatctg tcctggtaat cctcgtaagt tcaagtaagt tagactaatc acagagaacc
     5941 agccaagaag ttttacgatg aatgaatttt taaatcggct acccatctcc gcttggctgt
     6001 tggtcatcat cagtaaagga atcatcgaaa atggcaacgc aaaagccaag aacacctggg
     6061 attcattcat taagttattc aaagcggcat gttcttcgat cgttcctttt gtacttgtaa
     6121 acaaaacaca aatcaataca ggaatcacag aaagcaatcg tgtgaccagt cttcttagcc
     6181 aaatcgggat acgcatatgg atgaatcctt ccatgatgac ttgaccggtc aacgtaccag
     6241 taatagtaga gttctggcca gaagcaagaa gtgctacagc aaataaagtg gacaagatac
     6301 cagattttgc tacagcaatc aaaataccat tgctcatagc tgatggatca gataaagctt
     6361 catacagacc aaaaaaggaa ggatcttgga cagaaccggt tttgaagaca gcgacaccca
     6421 tgatcaatag cagtgcattg actagaaaag ccattgacaa ctggatatta gaatcccaag
     6481 tagaaaaacg tacagcttgt gctacatctt cttcgttttg gtgatcgatc ttccgagttt
     6541 gtgaaactgc tgaatgaaga tataagttat gcggcatgac tgttgcacca ataatcccta
     6601 aagctccagt cagcggagtt tcgccgccga tgaccggtga gtctgagaat gtatgactgt
     6661 tagggataaa gccagcaaat aaactgtgcc aatctgggtt agacaagcca acttgataga
     6721 caaaaacgaa taaaatgacg aaaattaagc agacaaccag tgcttcgatc ttacgaaacc
     6781 cgacttttgt cagtagtaag agaacgaaaa catctaatac agtaataaag acggcaatca
     6841 ccaatggaat atcaaaaagt aagtagaggg cgattgctgc accaatgact tctgcgatgt
     6901 ctgttgccat aatggctaat tcagtcaaga tccataaaat gacacccaat gtcttacttg
     6961 tccgtgctcg gattgcttgg gccaaatcca tttgggtcac gattcctaat tttgctgcca
     7021 tatactgtaa aagcatagca attagactag acatcaaaat aactgacatc aataaatatt
     7081 ggaaattttg tccaccagtg atcgaagtgg accagttacc tggatccata taaccaactg
     7141 ctactaatgc acctggtcca gagtaagcaa gaagtgtttt aaaaaaactt tttccctttg
     7201 gtacatccac tgttccattt acttcagcta aagataaacc atttgtatgt tcaataagtc
     7261 gatgcttatg aaattgtttg ttggattgat tattcaagaa aaaaccacct ttcctattta
     7321 tctacgatta cattctacac ctcagaaaga aaaaattaaa gaaaaagatt cggtatgcct
     7381 aatttttttt cgtggtgtgt cttgctggtt aatgaaagaa attttgttct tctctaaaaa
     7441 aaccactatt ttttgcatga aactaaagaa tacgcctttc ttttttcaat aaactttagt
     7501 aaaataaatc attgagagca attcggaaat agaaaggaga gatttatatg gtatcaacag
     7561 gtattgtcgt acttattgct attattgctt tgctaatcgg cgcagtaggt ggctttttcc
     7621 tagcgcgtaa atatatgaaa gactatttag aaaaaaatcc tcccgttaat gaggacatgt
     7681 tgcgatcaat gatgatgtca atggggcaaa aaccatctga gaagaaaatt cgccagatga
     7741 tgcagcaaat gaagaaccaa gggaaaaaat aggttctgac ccaaagactt tttgcgggct
     7801 gattttttca gtccgttttt tatttcgtaa tttgttagac tattcagact attcagcaaa
     7861 aaagacaagc acagacaatc ctatgctctt gataaagaca aaggaggagt aaaatgtcga
     7921 tttttagaaa attaggttgg ttttttcgtc aggaaaagaa acattattta attgggattt
     7981 tctccctatt cgcagtggcc ttggtacagt tagttcctcc aaaagtgatt gggatcatca
     8041 ttgatgagat cgccgataag aatttatcaa taaagatcat ttcctggtgg atcttattat
     8101 tgatccttgc agctttctta caatatcttt ttcgttatat ctggcggacg aatatttggg
     8161 gaagtgcagc tcggttggag agagaacttc ggcagcagct attcaaacat tttacagaaa
     8221 tggacaatct cttttaccaa aaatatcgga ctggagattt gatggctcat gcaacgaatg
     8281 atttgaatgc aatccagaat gtagcaggag ctggaatctt gacctttgca gattcagtga
     8341 ttacgggcgg aacaacgatc attgcgatga ttttgtttat tgattggcgc ttaacactga
     8401 ttgcgctgat tcctctgcct ttgctagccg ttacttcacg agtgctgggg tcaaaactcc
     8461 acgatgcttt tcgtgattcg caagaagctt tttctagtat caatgataag acacaagaaa
     8521 gtatcactgg gatcaaagtc attaaaacat ttggacaaga aaaagaagat ctcgcggatt
     8581 ttgaagaaaa gattgaagat gcaattatta aaaacaaacg agtcaatttt ttagactcct
     8641 tatttgatcc attcattacc ttgattatcg gtctgtctta tgttgcaaca attattttag
     8701 gtggaaagta tgtcttggaa ggcacgatct caatcgggca gatggtctca tttattagct
     8761 atatcggtat gcttgtttgg ccgatgtttg cgattggtcg attgttcaac gtattggaaa
     8821 gaggaaatgc ttcttatgat cgcgtcaatg aacttttgca tgaaaagacg catatcgtgg
     8881 aacaactggg tgcagtgcag accccggcaa aaggcgagct gaatgtagct atttcctctt
     8941 ttaactatcc gaaagaagaa tcaccagcat tagaacaaat caaatttgcg atccaagaag
     9001 gagaaacatt aggtattgtt gggaaaactg gtgcgggaaa aacgacaatc atcaaattac
     9061 tgatgcgtga atacgatgat tatcaaggga aaatccaatt tggcggacgt tcgatcaaag
     9121 aatattcatt gaatgcttta ttaggatcaa tcggttatgt gcctcaggat cacttcctgt
     9181 tttcgatgac cattcgagat aatatccgtt tttctaatcc aaaactttca gaagaagaag
     9241 tagagcatgc ggcttcttta gcatttatca acaatgaaat caaggaattg ccaaaaggtt
     9301 atgatacctt agttggagaa cgtggagtct ctttatctgg cggacagaaa cagaggattt
     9361 cgattgctcg agcattgatc aaaaatcctg aattgctgat cttagatgat gcactttctg
     9421 cagtagatgc caaaacagaa gaagctatct tgcataattt aaaagaaata agaaaagaga
     9481 aaacaaccat cattgcagct caccgtttaa gcagcgtgat gcatgcaaaa gaaatcattg
     9541 ttgtcgatca agggaaaatc attgaacgag gaacccacca cgagctgctt gcattaggtg
     9601 gctggtatga acgaatgtgg gaaaaacagc aattagaagc gaagatagaa ggaggcgagg
     9661 cctgatggat gaaaattatc aatcggaatg gacaaaatcg attccactta aagaacagaa
     9721 gaagatcatc aaacgactga tgaagttcgc caaacctttt agaagaacgt tcatcgttgc
     9781 tatcttattt gcgtttgccc tgtctgtgat caatgtccta ttgccaagga tcatccaaac
     9841 ttttatggat gatcatctgg ctaaacagtc agctacgaca caagttattt tgttttttgc
     9901 tggtgtgtac ttgtttggag tcatagtcaa aagtatcatt tggtttttcc aatggtattt
     9961 gtattccatg gcttcattga agacctatca gtatgttcgg gtaagattat ttgaaaagct
    10021 gcatacacta ggtatgcgtt attttgacca gacaccggca ggatcgaccg tttctcgagt
    10081 gacgaatgac acagaaacgc tatttgaatt ttggtatgtc tttttgatgg tattgactgg
    10141 gatctttgca gtcgtttctt ctttcgttgc catgtttcaa atcaatggag aaattgcctt
    10201 gtggactttg gtgttcttgc cgattttagg aatcgtcatc tggtattacc agaaattcag
    10261 ttctcgtatt taccgaaata tgcgggaaaa attaagtcag ctgaacacga aattgaatga
    10321 atcgatttcc ggcatgcgta ttatccagca gtttcgccaa gaagctcgtt tagctaaaga
    10381 atttgaacaa gtgaacaacg agtatttaga cacgcgctat gcgatgatca aaacgaattc
    10441 gattctgtta agtccaatca tcaattttct ctatgcattg gcgattgcgt tggcgttgac
    10501 tttgtttggg atcgatgcgt tgcattctcc cgtagaagtt ggactgatct atgcatttac
    10561 gacctatgtc caggaatttt ttaatccaat gtctcaaatg atggactttt tgagcacctt
    10621 tacagatggg atggttgccg gtagtcgtat tttgaaaatc atggatacag aagaatatgc
    10681 accgcaacaa aatcctggag cagatcaagc gattacttca ggtaagatcg agtttcgcaa
    10741 tgtaagcttt tcttatgatg gagagcacaa tgtattgaac aatatctctt ttgtcgctaa
    10801 tccgggtgag accgttgcat tagtggggca caccggaagc ggaaaaagct cgatcatcaa
    10861 cgtattgatg cggttttacg aattttatga aggccaaatc ttgattgatg atcatgacat
    10921 tcgagaatat ccaatggaag agttacgtaa gaaaatggga ttggtcttgc aagatgcctt
    10981 cttgttttat ggagatattg caggaaatat ccggatgatg aatccagcta tcacagacga
    11041 acagattcgc catgcagctg agtttgttca agcaaacaag ttcatcgatg aattaccaaa
    11101 aggatatcac tcaaaagtga tcgaacacgg tgccagctat tctagtggac aacgacagct
    11161 gatcactttt gcgcgaacaa tcgtgacaga tcctaaaata ctagtattgg atgaagcaac
    11221 agcaaatatc gatactgaaa ccgaaggatt gatccaagaa ggattggcaa aaatgcgaca
    11281 aggacggaca acgatagcga ttgcccatcg gctgtccacg attcgggatg cagaattgat
    11341 tttagtttta gacaaaggtc atatagtaga gcgtgggaat catgaaacac ttctagcgca
    11401 aaatggtttg tatgcagata tgtacaaact gcaaagcagt ggtgtgtaaa aagaggttgt
    11461 gacagtagca tagtagcatt ttgccctaag cgctagagtg aaaataaaac agagcttcgc
    11521 agattcttca tctgtgagcg ttagtggcgt agtcacccga tttaaaaaag acgacgacag
    11581 ctagtcaaaa gcggctaata accactaaga aagcgttgac aaaagcgttt gctttcgata
    11641 atataaagaa aacgatacaa cttggatagt gattgcctga tgcaagctaa acctgatgag
    11701 tctcccatgg ttccatggac ggctttttgg gtttttcttt ttgttttgga tacagcccta
    11761 tatatcatgg aaaggagcaa aacatgctga ttgagaaagt actgaataac aacgtcgtaa
    11821 taaccaggga tgaaagaaat caggaaatga tcgtgatggg aaaaggtttg gcattcaaaa
    11881 agaaagtggg tgacccgatt tttgactcat gcatcgacaa aaaatttcag ttgacaaatg
    11941 acgggctttc acaacagttc caagaattgg cacaagcgat tcctcttgag tacttagaaa
    12001 tttcttgtga aatcattgaa tatacaaaag aggctctaca agtccagttg aatgattctg
    12061 tgtatattgc attgacggat catttgtata cggctatcga acgtcacaaa atgggaatat
    12121 ttgtaccaaa tgtattgctg tatgatatca aacaactatt tcctaaagag tatgccatcg
    12181 ggaaaaaaaa cagtgaagaa aatctcggat aaataccaca tcaagttatc agaagatgaa
    12241 gccggattta tcgcattgca tttagtcaat gcacaaggca ttcaaagcca acaagacaat
    12301 acctacctgt tcactataac aatacaggat attctaaata tcgtccgcta ttactataag
    12361 attacatttg atgaagattc agtccatttt tttcgattcg tcacacactt acgttttttt
    12421 ctgacacgcg tcctagatgg agaaaaatca gaagtgggag aagtttctga ggaattgttt
    12481 caggttattc aaaaaaaata taaggatacg tttggttgcg ttgagaaaat agcagacttt
    12541 ttagccgaga cgttcaacta ccagatgtcc acggacgaaa aaatgtattt aacgatccat
    12601 actgcgcgtt tagtgcatga gaatcgttgt aaataaataa aaaaaggagg ataaattaac
    12661 aagaacgtat gaaataaggg tagtgacatt cagttggcta aaccttccac taataaattg
    12721 gagggaatag aaatgggaaa ataccaagaa ttagcgagaa aaatcgtaga aaatgtcggc
    12781 gggaaggaaa atatcaatgg tttgacacat tgtatcacaa ggcttcgttt caaattaaaa
    12841 aacgaagaaa aagcaaatga tgagatcttg aaaaatatgg acggtattgt aacggtaatg
    12901 cgagcgggag ggcagtatca agttgtcatc gggaatcatg tgccagtcgt gtttgaagag
    12961 gttataaaag caggaaacct tacttttgat gaggctgttt caacagaaaa aatgagaccg
    13021 tttgatatgc ttattgatgg aatatcgggc tgtttccaac cattccttgc tatcctagct
    13081 gctgacggga tgattcgtgg actgacagca ttccttgtct tcttaggcgc gtttgaccgc
    13141 ggttctggca catttgttat gtttgataat atcggtgatt ctgtattcca atttatgcct
    13201 gttattatcg gtttaacagc tgcccgtaaa tttaaagtaa atgaattcgt agggatgttg
    13261 cttggcgcag cattaatgaa tcctagcttg tcgctggaag ctttgagtgg tgcagcagaa
    13321 gcaccactaa cgaccatatt ttctggcact atttttgaag cacctattta tcaaacagta
    13381 ttcggcattc cctggattgc gcgtaactac gcttcttctg tcattcccat tatctttatt
    13441 gtgttgttag ctagccaaat tcagaaacca attaaaaagt tagtacctga gatgatcgcg
    13501 aatttcttcg ttccattctt caccgttctt attacaatgc ctttaggttt tttactcgtt
    13561 ggaccagtat tcacattcgc aactgatatt ttaatggctg gatttgaaac acttttagca
    13621 ctttcgccag ttatttatgg agcaatcgta ggcttcttct ggcaaattct ggtgatgttt
    13681 ggtctccatt gggcaatcgt accaatggga ttgatgcaat tttctgtgaa tggttggcaa
    13741 aatattatga cacctgttgc agtagtgtct tttggacaaa ctgcagcact taccgcactc
    13801 tactttaagc ttagaaatcc aaaagacaaa gcaattgcaa taccagccat tgtttctggt
    13861 attgtgggga ttacagagcc agctatttat gggtttacat tgccacgtaa aaaaatattt
    13921 atttttacct gtgtcggtgg tgcgttaggg ggcgcgtata gtgggttaat gaatttaact
    13981 tcttggaatc aaggtggact aggcatcttt acaattccca actatattag accagatggt
    14041 gatttaacag atgtgattaa tgtattgatt gggatcgcca ttgcgatggt ggtatccttt
    14101 gcgctgactt ttttcttttg gaaggaggaa gcagatgaaa cagatgatag acagaaaaga
    14161 tcaggtaaag aaattgtaaa aacaccgata caaggacaaa tcgctccact gaatgcagca
    14221 aaggatgcag cttttgcaca aggaacacta ggtcgaggaa tcttgatcta ccctgaaaaa
    14281 ggagaagtac gtgccccttt tgacggaaca gtcatgtccc tgtttccgac gaaacatgca
    14341 atcgggatgg tttctgaagc aggtctggaa ttgctgattc atgtcggatt ggatactgtt
    14401 cagttggaag ggaaatattt tgaaagcttg gtgcagcaag gagaaagagt gaaacaaggc
    14461 gatctgttgc ttcgcttcga caaaaagaaa atcgaagagg aaggatacag tttagaaaca
    14521 cctgtgattg tcatcaatta catggattat ttggatattt tggaaaatca aggggaaacg
    14581 gtcgggccag acgatgcact tatcaccgca ctgacctgaa cggttaggtg aaagaatgga
    14641 ctaggcttaa ctattctttt tagtccactt ctttattttc aaaattgaaa ggctctttca
    14701 tgatggctga gctgataggg cgtttatcaa atttttgtcc tacagaaatt agatggacta
    14761 aattctgttg ataattttct gcaaaaacac aactatataa cacgtctaga agataacaaa
    14821 tcgatgtatt gatcgtaaaa ttaccaattt tcgaatacag tcgttcgcgt gtcgtaagtc
    14881 gcaaagcggc tgtactcatt gaagatagcg ttgaatcacc gatactggtc aaagctaaaa
    14941 ttggcacatg attttgtttc aatatttctg cggtacgaag gatcacattg ttttcacccg
    15001 tataagaaat cagaatccca cacatatcac ttgtgcaatt gtacgcttca tagaacgatt
    15061 caccaaaagt ggtgacaatc gtgacatcca ttttgatgcg cttcattttc agcatgaaat
    15121 cttgagaaat caaagtattc gagttactgg cgaaaatcac gatttttttt gcttgaagca
    15181 ttagttgttt ggctttttgc agttggtcat gatgaatgag agaaagtgta tcttgaatcg
    15241 tcatctgttc taaagaagcc attttatttg cgatcgtcat aataccgtct gtggtggaaa
    15301 aaggaagatt cgcatctacg tcttgaaaat tcccttcaag ataggctgtt tcagctaaaa
    15361 aagcattacg aaaactgacc cagccatcaa aacccatttt ttttgctaca cggatgagtg
    15421 tggacgggtg gacataacat cgctcagcaa tctcttggat ggtcatttcg ccaagtgctg
    15481 gagaatcgct taagagaaac tgaatcactt cattttctga aggagaaaag gagatttctt
    15541 tgattttttc acgtaataac atgtagatac cttcttcttg tttttttacg taaatcataa
    15601 cttttttgaa aatgatttgt aaacggattt atgttatata gcttaataaa gacatttttt
    15661 gacaagatat ttacaaaaat ccagaagaaa acactaactt ttgtaaatgt tactggaaag
    15721 aaacgaattt ctattgtttg ttttgtttac aaaaaagata ataaaagtgt aaaaagtaaa
    15781 ggagtgagaa catggcaaaa ggaatcaaaa tcgctacgat tggtggaggc agcagttaca
    15841 cccccgagtt gatggaagga tttatcaaaa gatatgagga attaccgata cgagaaatct
    15901 ggctcgtaga tgtagaagaa ggaaaagaaa agcttgagat cgtcagtcag atgtctcagc
    15961 gtatgtggga tgcttctccg tatgaagtca aaatctatgc aactctggat cgggaagaag
    16021 cattgaaaga tgcggatttt gttacgactc agtttcgtgt cggtcaatta gatgcacgaa
    16081 tcaaagacga gcggatccct agttattatg ggatgattgg gcaagaaaca aatggtgctg
    16141 gcgggatatt caaagctttt cgtaccgttc cagtggtttt ggcaatcgtt gaagacatga
    16201 agcgtttatg tccaaatgct tggctgatta atttcgcgaa cccaagcggg atggtgacag
    16261 aagcagttat cagatatggc aaatgggata aagtgatcgg attatgcaat gtgccagtaa
    16321 tggcgatgat gacagaacca acaatgcttg gcaagaaaaa agaagagctg atctacaagt
    16381 ttgccggtct aaatcacttc cattggcaca aagtagcaga taataaagga aatgaccgca
    16441 cggcagaatt gattgatcgt ttatacgatg aagacaatgg attgccgaaa aatatctttg
    16501 acatcccgtt tttcaaagaa caattaaaac aaatgaacat gatcccttgt ggctaccacc
    16561 gctattacta tcgtgaagaa gaaatgctgg aacatgcttt agaagaatat caaacgattg
    16621 gtacaagagc gcagcaagtg aaagaaacag aagcagagct gtttgaatta tacaaagatc
    16681 cagaattgga tcataaacct gaacaattgc aaaaacgagg tggtgcttat tattcagatg
    16741 ctgcctgtga aacgattgct tcgatctatg gaaataaaga aacacagatc gtggtatcta
    16801 caaagaatga tggagctgtt cctgatctgc cggcagattg tgtagtagaa gtcactgcct
    16861 atgttggcgc acaaggcgca cgaaatgtag cgtttggttc ccttcctgct gctgaaagag
    16921 gctggttgca agtaatgaag aatatggaat tgctgacgat cgaagcagcc gtgactggtg
    16981 attatggaac agctttgcaa gcgtttacga tcaatccgct tatacgatca ggaaagacag
    17041 caaaacggat catggatgag ttgtttattg cacatcagca gtatttaccg caattcaagg
    17101 aaacaatcag ccggttaaaa gcagaaggaa tcgaagtcca agatgaactg gcaagagaat
    17161 tatcaagcaa tacacatgta atggaggaag tatgatggga ttcagaaaag attttttatg
    17221 gggcggagct acagcagcca atcaatgcga gggcggttat gatgaaggcg gacgtgggtt
    17281 agcgaacgta gatgttgtgc caatcggaga agaccgtttt cctgtcatta ctggaaaaaa
    17341 gaaaatgttt gaatttgatg aaaatcattt ttatccagca aaaattgcga tcgatatgta
    17401 tcatcattat aaggaagata ttgctttatt tgcagaaatg gggttcaaga cttatcggat
    17461 gtccattgct tggagtcgta tttttccaat gggcgatgaa aaagagccaa atgaatctgg
    17521 gttgaaattt tatgaggatg tctttaaaga atgtcaaaag tacgggatcg agccgcttgt
    17581 gacgattaca cattttgatt gtccgatgca tttgatcaaa gaatatggtg cgtggcgttc
    17641 cagagaactc gttggttttt atgaaaatct gtgccgagtc atcttcaatc gctataaagg
    17701 acttgtcaaa tattggttga cattcaatga gatcaatatg atccttcatg caccattcat
    17761 gggcgcaggg ctgtattttg aagaaggaga aaatgaagaa caaatcaaat atcaagcggc
    17821 acatcatgag ttggttgcaa gtgccatcgc aacgaagatc gctcatgaag tagatccgga
    17881 gaacaaagta ggctgtatgc tggcagcagg agcatattat ccatacactt gcaatcctga
    17941 agatgtttgg gcttctagaa agtcagatcg agaaaattat ttcttcattg atgtccagtc
    18001 acgaggagaa tatcccgctt atgcattgaa agaaatggaa cgaaatggca ttcagatcaa
    18061 aatggaacct ggggatgccg cattgctgaa agaacatact gttgatttta tctccttttc
    18121 ttattattct tctcgagttt cgactactga caacgaactc ttggaacaga ctgcaggaaa
    18181 tatctttgct tctgtcaaaa atccatatct ggaagcaagt gaatggggtt ggcaaattga
    18241 tccattaggc ttgagaataa cgatgaatga tatttatgat cgttatcaaa aaccactatt
    18301 tattgtagaa aacggcttag gtgcagtcga tacgcccgat gagaatggtt atgtggaaga
    18361 tgattatcga atcgactatc tggcagcaca tatccaggca atgaaagatg cagtagaatt
    18421 agatggagtc gatctattag gctatacgac ttggggatgt attgatttag tatcagctgg
    18481 tacaggtgag atgaagaaaa ggtacggatt catttatgtc gatcgtgaca acgaaggaaa
    18541 tggcacatta gcccgttcga agaagaaatc attcaactgg tataaaaaag taattgattc
    18601 aaatggagaa aatctttcga acgattgatc aatgatttat aaaagtctat tgtttttcag
    18661 cggatttttc gagtataata aagctattcg agaaacgcaa gcagaacaat agactttttt
    18721 tcgcattggc tgtaaagtat gcttacttga ttaagtaaag gagaatctac atatgtatga
    18781 tgtaatcgtt attggcgcag ggcctgctgg gatgactgct gctttatatg cttcacgttc
    18841 aaacttatcc gtattgatga tcgaacaagg cgctcctggc ggccaaatga acaatacggc
    18901 agaaatcgaa aactatcctg gtttcgattc aatcatgggt cctgatctag cttataagat
    18961 gtatgaaggt gtttcaagat ttgggacaga aaatgcttat ggaatcgtgc aagatatcaa
    19021 ggatcacggc agccacaaag aagttatttg tgaagataaa acttacgaag gaaaaacagt
    19081 cattatcgca acaggctgta tccaccgtaa attaggcgta cctggcgagg aagaatttgc
    19141 tggacgtggc gtttcttact gtgcagtttg tgatggtgca tttttccgta acaaacgtct
    19201 agtagtgatc ggaggaggag actcagcagt agaagaagcc atctatttga cacaatttgc
    19261 ttctgaagta gtcatcgtcc accgtagaga tgaattacgt gcacaaaaga tcatccaaga
    19321 ccgtgcattt gcgaatgaaa aaatttcctt tatgtgggat acagtcgttg acgaaatcgt
    19381 tggaaatgaa atggtagtga caggtgtgaa agcacgtaat gtcaaaactg gcaatgagtt
    19441 tgatttggca gcagacggag tattcatcta tgtaggattg gatccattaa ctgcaccatt
    19501 tagagaagct gggttgacga acgaagccgg ctggattcca acagacaacg aaatgaagac
    19561 gaatatacca ggcgtttttg ccatcggaga tgttcgtgaa aaagatttgc gacaaatcac
    19621 tactgctgtt ggtgatggtg ggattgctgg acaacaagtg tataaatact tggaatcttt
    19681 agaagaaaca actgcataag tttaaaaaga gtgaaagaac ggtttcaatt gttcttttac
    19741 tctttttttt gcgtgaaatc tactgaatcc gatttatgcg aatacttgta accgtgcttg
    19801 ataaaagaat cttttcttga tttaagcgtt ttttggtcct catactttca tatctaataa
    19861 gtgaatgcta tacttattaa gaaccatatt tcagaaagag gtgttatttt catgtcttgg
    19921 gaacaagtgt atgaacaatg ggtacatgaa gaaaatttag aagaaaactt aaaaaaacaa
    19981 ttaaatgatc tgggtgaaga tcctgaaaaa cttgaagatg ctttttatgc accgttggaa
    20041 ttcggtactg caggcatgcg gggaatctta ggacctggga tcaatcgaat gaacatctat
    20101 acggtaagac aagcaacaga aggacttgca cgtttcatgg atacacaagg tgaagaaaca
    20161 aaacgccgag gtgtcgctat cgcttatgac tcacgccatc aatcaccaga atttgccatg
    20221 gaagcagcca aaacattagc acatcacaat atcccttctt atgtattcga aagtttacgt
    20281 ccaacaccag agctttcttt tgcagtccgt catttgaaga cgtttgcggg aatcatggta
    20341 actgcatccc acaatccagc agcttacaac ggatataaag tctacggaga agatggggga
    20401 caaatgcctc ctgcagacgc agatgcattg acgaaatatg tccgtgaagt cagcaatccg
    20461 ttaaaagttg aagtattatc tgacgacgaa gctgaacata gcggattgat cacgatcatc
    20521 ggagaagaag tggatgccgc ttatctagaa gaaatcaaag cagtaacgat tgatcatgaa
    20581 ttggtggaaa caatgggtaa agatttgaag cttgtgtaca caccacttca cggaacagga
    20641 aaaatgttgg gcgaacgtgc gttgaaacaa gctggattcg aacaattcgt actagttcca
    20701 gaacaagctg tcgcagaccc ggatttcact acagtcaaat caccgaatcc agaagaacac
    20761 tctgctttcg agtatgcgat gcgacttggc gagaaagaag gcgctgactt actgattgca
    20821 acggatcctg atgcagaccg tttaggcgca gccgttcgtt tgccaaatgg ctcctatgaa
    20881 gtattgacag gaaaccaaat cggagccctg tttgctcaat atattttgga agcacaccaa
    20941 tcgcgaggca ctttacctga aaatgcagcg gtattgaaat caatcgtttc tagcgaattg
    21001 cctacagcaa ttgcggaaca ctatggggct accatgttca atgtattgac aggattcaaa
    21061 ttcattgctg aaaaaattca acaatttgaa gaagatcatt cccatacttt tatgttcgga
    21121 ttcgaagaaa gttacggtta tttagttaaa cctttcgttc gcgacaaaga cgcgattcaa
    21181 gcattgctct tattagcaga agtagcagct tactataaaa aacaaggcaa aacattgtat
    21241 gacggcttgc aaacgatttt tgaaacatat ggctactacg cagaaaaaac gatttctgtg
    21301 acaatgagcg gacaagaagg ctctgcaaaa atctctgctt tgatggaaaa attccgtgaa
    21361 gaagcaccga cagaatttgc cggagcaaaa gtcatccaaa cagaagactt caaagagttg
    21421 acgagaacag accgtgaagg aaacgtagaa aaactaacga caccgccatc tgacgtattg
    21481 aagtatacgc tggaagaaaa tggctggatc gctatccgtc cttcaggaac tgagccgaaa
    21541 atcaaatttt atatcggtgt aaaaggaaac tcttcagaag acgcagctga aaaagtagcc
    21601 gcttatgaag cgattatcaa aaaaatgaca gaagaataaa aaaacttgag actgatggaa
    21661 aaagtggtaa actttagaag tcagtctttt ttcaagttgg aggtgaatgg attgccagac
    21721 acgcaaaaaa caaaagtaga gaaagtcagc caactattca aaatgttgag tgaccccact
    21781 cgtctcaaaa ttctgctgta tctgaaagac ggggaacaaa atgtgacagc aatcacccaa
    21841 gcagtagaaa tggaacaatc tgctgtatcg catcaactgc gtttattaag agaaaaccat
    21901 gtcgtcaagt cccatcgtga gggaaaagcg attttgtata gtcttgatga ttctcacgta
    21961 ctggatattt tgaaccagac gctcaaacat gtggaacatc aataacgata ggtgcaaata
    22021 gagacaaact ggactgtatc aacgaatctt cttcgctgac acagtccagt ttttttaaga
    22081 aaaagacaag aaaaactgtt agtctgtttc ttcggtggtt tcagttccat aataactatg
    22141 atcttctagg tctaaagcat tcaaaataag tgaagctgtt tcttcgatag ataaagaagc
    22201 gacattgatt actacgcagc ccaatttttc ataaagatca ttcgcaaaat cgagttcagc
    22261 tcgtattttt tcgatatcgg aataagcagt atcaggattt aatccgtaag agcgcatccg
    22321 ttctttacgg atattgttta aaatatctgg gttattcgtc agtcccacga tttttttagg
    22381 atcgatctcc caaagctgtt tgggaatatg tgcttgtggg atcaaaggta gattggcaac
    22441 ctttaaattt ttgttagcta aaaacaagct caaaggcgtt ttggaagttc tggatacgcc
    22501 tagcaaaacg acatctgctt ctagaaaccc acgaggatct ttcccgtcat cgtattttac
    22561 agcaaattcc attgctttga tccgtttgaa atagttctca ttcaataaat gaagggcgcc
    22621 aggttgccga atgggcttga taccagttaa gcgctcgatt tctgatatcg gcggagtcat
    22681 cacatcaaaa ctaaataatt gatgttcttc acaaaaagaa cgcgtcatct cgatcaattc
    22741 ttgattgatc aatgtgtgta ggacaagggc tttttctttt agtgcatctt gcagtgcctg
    22801 catcaatgtt ttctcatctg tcacaaatgt tctgcggaat aaattgaact caacggtagg
    22861 atattgggcc atagtggctt gcgctaattt tgtcgcagtt tctccagcag agtcagagat
    22921 gacaaaaaaa gtaatgattt ctgtagaatc ggaagacatg gataaaacct cacttgcatt
    22981 ttttttatgt ttattatacc atactctaga atgtgtactt cgtaatcatt ttgatttgga
    23041 ggaataagtc atgaaaaaac agacattgat tgaacaatca ctagaaacat tgaaaaataa
    23101 cggttttaaa tatactaaaa aaagagaagc attgttaact tatttagtga agcgcaatcg
    23161 ctacgtatcc gctaaagaag tttttgacta tatgaatcag gaattcaaag gagtaagtta
    23221 cgatacgatt tatcgaaatc tccatgattt tgcagaaatc gatttactgg aagaaaccga
    23281 tttaaatgga gagatgaagt ttcgttttca ttgctgtcaa ggggaaatcg gccatcacca
    23341 tcactttatc tgtacggtgt gcggaacaac gaaagaaatc catatgtgtc caatgaatta
    23401 tttcgaagaa cagctcggtg gatgtacgat cgaagggcat agattcgaga ttttcggaag
    23461 atgtgaaaat tgtcaataag gatttaaata aaataacatt tgataatttt aaaaaaaagc
    23521 aagatttcct tgacggtcaa aacagcatta gatataatat ttacaggaca gtcttgtttt
    23581 ttgagatata tttcaataac aattacggtt actttttaag ctcggaggga gggaattaac
    23641 atgtcaaaaa cagtcgttcg caaaaacgaa tcacttgacg atgctcttcg tcgcttcaaa
    23701 cgttccgtgt caaaagcggg tactttacaa gaatctcgta aacgtgaatt ctacgaaaaa
    23761 ccaagtgtaa gacgcaagaa aaaatctgaa gcagctagaa aacgcaaaaa attctagttc
    23821 tgtgatgaat tggagttggt agtgtgtcac tactaagtag attaaacgat gacatgaaaa
    23881 cagcgatgaa agcgaaagat aaggaatctt tacaagtcat ccgaatgatc aagtcatcta
    23941 tacaaaacga gcagattaaa gaaggtcacg acttgacaga agaagaagaa ttaacggtgt
    24001 tgtctcgcga aatgaaacag cgacgtgatt ctttgcatga gtttgaagaa gcaggacgtg
    24061 acgatttagc tgaaaaagta aaaagtgaaa ttgtgatagt cgaaaaatac atgccggaac
    24121 aactttccga cgaagaaatt cgacaacttg ttcaagaagc aatcacacaa actggtgctt
    24181 catcaatgaa agaatttggc aaagtcatgg gtgcgatcat gcctaaagtc aaaggaaaag
    24241 cagatggaaa ccaagtaaat gccattgtga aagaactatt acaatcttaa ttttatcatt
    24301 taaaggctct tcgtcaatga gtgttggtga agaaattc
//
LOCUS       DAZKNH010000035        23645 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000035 DAZKNH010000000
VERSION     DAZKNH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.035"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACG8MG_001320"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACG8MG_001320"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFQ0375375.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="ACG8MG_001321"
     CDS             complement(1174..1869)
                     /locus_tag="ACG8MG_001321"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFQ0375376.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="ACG8MG_001322"
     CDS             complement(1882..2598)
                     /locus_tag="ACG8MG_001322"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFQ0375377.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="ACG8MG_001323"
     CDS             2928..3536
                     /locus_tag="ACG8MG_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFQ0375378.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="ACG8MG_001324"
     CDS             3861..4184
                     /locus_tag="ACG8MG_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFQ0375379.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="ACG8MG_001325"
     CDS             4366..5736
                     /locus_tag="ACG8MG_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFQ0375380.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="ACG8MG_001326"
     CDS             complement(5866..7167)
                     /locus_tag="ACG8MG_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0375381.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9512)
                     /locus_tag="ACG8MG_001327"
     CDS             complement(7521..9512)
                     /locus_tag="ACG8MG_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFQ0375382.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="ACG8MG_001328"
     CDS             complement(9656..9922)
                     /locus_tag="ACG8MG_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0375383.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACG8MG_001329"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACG8MG_001329"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFQ0375384.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACG8MG_001330"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACG8MG_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFQ0375385.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /gene="def"
                     /locus_tag="ACG8MG_001331"
     CDS             13266..13829
                     /gene="def"
                     /locus_tag="ACG8MG_001331"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0375386.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14082..14603)
                     /locus_tag="ACG8MG_001332"
     CDS             complement(14082..14603)
                     /locus_tag="ACG8MG_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFQ0375387.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14606..15448)
                     /locus_tag="ACG8MG_001333"
     CDS             complement(14606..15448)
                     /locus_tag="ACG8MG_001333"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFQ0375388.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15441..16166)
                     /locus_tag="ACG8MG_001334"
     CDS             complement(15441..16166)
                     /locus_tag="ACG8MG_001334"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFQ0375389.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16342..16638
                     /locus_tag="ACG8MG_001335"
     CDS             16342..16638
                     /locus_tag="ACG8MG_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375390.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16894..17068
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17206..18798
                     /locus_tag="ACG8MG_001336"
     CDS             17206..18798
                     /locus_tag="ACG8MG_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0375391.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18811..19551
                     /locus_tag="ACG8MG_001337"
     CDS             18811..19551
                     /locus_tag="ACG8MG_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375392.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19719..21068
                     /locus_tag="ACG8MG_001338"
     CDS             19719..21068
                     /locus_tag="ACG8MG_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0375393.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21196..21786)
                     /locus_tag="ACG8MG_001339"
     CDS             complement(21196..21786)
                     /locus_tag="ACG8MG_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0375394.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21933..23285
                     /gene="brnQ"
                     /locus_tag="ACG8MG_001340"
     CDS             21933..23285
                     /gene="brnQ"
                     /locus_tag="ACG8MG_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0375395.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6771 a         4761 c         4503 g         7610 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagtcatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaaata aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaaa
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc
    13921 tccactaatc gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat
    13981 tggatacgca tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga
    14041 tgatagaata gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt
    14101 cctgcttcag ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc
    14161 gtatgcagat ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa
    14221 cgtctcataa aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca
    14281 ccgtaaaggg gatgttccac aattacatca atgatttcac caccattttc aacgattagt
    14341 ctcagttctt cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca
    14401 gtattggttt cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct
    14461 gcagctcgta gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc
    14521 gcaaacttgc tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt
    14581 cgccgtgtct ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg
    14641 agtgtttcat caaatcaaca tacgtatctg ttattgtttc cttatatccc ttatttttca
    14701 gcagactaac aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt
    14761 tttcttcttt tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag
    14821 caatcaactc tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg
    14881 ttagttcttc ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt
    14941 cgggaaaaga tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga
    15001 cacccggcgt gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt
    15061 ttgcgtactc cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa
    15121 gctggacatt cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa
    15181 atgtacaatt gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa
    15241 aattgataac tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca
    15301 attgggatcg cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa
    15361 acatacggtc ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt
    15421 tttctttttc ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa
    15481 acaaccgctc cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga
    15541 ttgattacta agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga
    15601 acttgaaaac tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc
    15661 tgctccatga ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt
    15721 cctccgcatg cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga
    15781 cattttcgac aatgacattg atacaaactt ccatgaaaat tgacgagttg cgggctttta
    15841 gcctttgcgt gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct
    15901 agatgtgcca tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg
    15961 acaaattgat agaatttatc cggttcattc accattgctt gatgactcag caagtattcc
    16021 ggctgctcca tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt
    16081 gtggataccc ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct
    16141 gcttcgtcta ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac
    16201 tcgaacaaat ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt
    16261 tgaacaattt atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt
    16321 ataaaagtga ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct
    16381 tgtatcgcct ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa
    16441 aatggaaatc tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt
    16501 acattagccg tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat
    16561 ctaatattag ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca
    16621 aagaaagcgg cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag
    16681 ttcttttttt attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga
    16741 tagatgattc acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt
    16801 attttttcaa aaaacagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt
    16861 ttgtataaat aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat
    16921 taaccctgtg gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg
    16981 cacttttttg cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc
    17041 agtgatgcta agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg
    17101 cctttttata tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt
    17161 catctagacc gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg
    17221 gtttcagttg ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca
    17281 aatgcagatg aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca
    17341 cccttcaact ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat
    17401 agttacgccg gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa
    17461 aaattagtca tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag
    17521 atcgacgcga tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgatttt
    17581 accgatcctt attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca
    17641 aaagcaaaaa gtttagcaga tctttctgga gcaaagatta ctgctcagtt aaatacattc
    17701 cactatactg tgatcgatca gataccagat gtgcagaaac aacaagcaat ggataatttc
    17761 tcagcaatga gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg
    17821 gaaggtgtaa cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca
    17881 gatggcttcc agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac
    17941 ccggatatcc aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa
    18001 attatggacc aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc
    18061 atcatttctg atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct
    18121 ggacttacct tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg
    18181 attggtgtct tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa
    18241 atcggaaatg cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta
    18301 caagcaatgg tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg
    18361 attgccgctt tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt
    18421 ggggggattt ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc
    18481 catggccaaa cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca
    18541 acaggaaatg agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta
    18601 gctgatttgt tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca
    18661 tttacgatcg tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg
    18721 gcggtagaaa agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat
    18781 tcaaatttgc aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat
    18841 ctaagaaaaa gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa
    18901 ggtgaagttg tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt
    18961 atcaatttac tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta
    19021 gctcctaaat ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc
    19081 aacctgttca acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg
    19141 aaacgagata aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg
    19201 gatcgttata tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca
    19261 attgctcggg cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg
    19321 ttagaccctg aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc
    19381 ttgacaatgc ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc
    19441 atttttatgg ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat
    19501 ccgaaagaag aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag
    19561 ctgattggga caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa
    19621 aggtaaattt ctgaaaattt tggaatctta gttgtgtttt tcagaaaact cttttaatat
    19681 ggaagtaatt aaactttttt agttaggatg atttaaaaat gaatgaagag caagaactgc
    19741 agcgtggact gaaaaataga catgtccaac tgatttctat tggcggagcg attggaacgg
    19801 ggttgttttt aggttccgga aagtctatcc agctagctgg cccttcgata ttgctcgcct
    19861 atcttattac tggatgcatc tgttttttca ttatgcgtgc gttaggcgaa cttctgttat
    19921 caaacaccaa taaccattct tttctggatt ttgttgcaga atatcttggc aaaaaagcgg
    19981 ctttcatcac tggctggacg tattggttct gctgggtatc gattgcaatg gccgatttaa
    20041 cagccatcgg aatgtatgta cgctactggg cgcctggtgt tcctcagtgg cttcctgaac
    20101 tgattgcatt aggtcttctt ctttgcttga atatggtggc tgtctcattg tttggtgagt
    20161 tagagttttg gtttgcattg atcaaagttg tagcgatcat tgcctttatc ctcgtaggag
    20221 cttatatgat cctcacccac tatacgacag atattggcac agccagtatc acgaatctat
    20281 ggtctcatgg cggattcttt ccaactggtg caaaaggatt tatcttagct ttccaaatgg
    20341 taacctttgc ttttgctggg atagaattag ttggattagt cgctggagaa acagaaaatc
    20401 ctgaaaaagt gttgccggaa gctatcaata atattcctat caggatcatc ttattttact
    20461 taggatctct ttttgtcatt atggcaatct atccatggaa cagcttgaat ccagataaca
    20521 gtccatttgt agaagtattt tcggaaattg ggatcacgat tgctgcttcc ttgatcaatt
    20581 tagtcgtatt aagtgcagca gcttctgcct gcaacagtgc aatatatagt acaggacgta
    20641 tgcttcgttc tttagcgcaa gaaggcagtg cccctaaaaa attcaaaaag ttaacaacac
    20701 atcatgtgcc agggaatgct ttgacatttt ctactatcgt cattttcatt tctgttatct
    20761 tgaattacgt gatgcctagt gaagtattca cactagtaag cagtattgcc actacttgtt
    20821 tcttgtttat ctggagtatg cttgtctaca cgcacatgaa atatcgtaaa agtatcctcg
    20881 gcaaaaaagc tcattcattt aaaatgccgt tgtacccatt tagtaattat ctggtatttc
    20941 tttacatggc ctttgtgtgt gtcgtcttgt tcctaggaaa agatacgaga atcgctttat
    21001 tactaacacc tgtgtggttc ttgcttctat tactaatcta tcatatgaaa tatgaacgta
    21061 agaagtaaga aagtagttgt gacagaagtg gtcagcttcg agaaataaga aggaatttac
    21121 gaaaattgct tcttaaattt ttgtgaaatt tcggcttatt tcttggagtt aaaagcttct
    21181 gtcccagcct cttttctatt tccccggaaa caaccgctga taataggcat ctgctttttt
    21241 ataggaaaaa atccaaatac cagatacaaa aaaacgtccg aataacccga tcaacggtac
    21301 aaaaaagaca ccggcaatca agcataagtt aatcatcatg gagataagac ttcgacggtc
    21361 ctttgacaaa actctcacga catgggaatc tggtttattt gctttcatga tttgcctcat
    21421 caagatacca aaagaaaaac tccacatcgt gtatactaag acataagcca tctcaggcca
    21481 aaaagcttca gggttacgcc cgacccaact agtaacagaa ggacataaag tcagccaaaa
    21541 gagcaaaaag atattcgacc agaccactcg gtaattaatt cgttctgcca tctgaaatat
    21601 acgatgatgg ttcacccaga tcaccgcaac aaatataaag ctgatggtat aagctaataa
    21661 tgtatttcgg atgacccata aagaatgaaa gctagatgta ttagggatct tgatctctaa
    21721 tacaagaact ctcaaaacaa tggctaccac tccatcagta aatgcttcta atctcgactt
    21781 tgtcacgatg attcctcctc agatgattca gcgcttttat tttatcactt acgttattaa
    21841 ataaccgtcg ttttatatag gaaattttct gaaaattgtt gcatattttt cgaaattttg
    21901 ttacttttaa aaaaaccgta aaggagttat gtatgacaaa aaaattatca tttaaagact
    21961 atctttttat aggctccatg cttttcggtc tattttttgg tgctggaaat cttatctttc
    22021 ccgttcattt gggtcaagaa gcaggcgctg ctaccttttg ggcgaatcta ggtttcttgg
    22081 ttaccggtat cggtcttccc ttcttagggg tgattgccat tggtgtatcg aaaagctcag
    22141 gtgtatatga attagcaaaa cggataaatc aaacatatgc gatggttttt accatcttgc
    22201 tttacttagt tatcggaccg ttttttgctt tacctcgcct tgcaaccaca tctttcgaga
    22261 ttggtttagc accatttatc gatcctagca gacagacgct atatctagca attttcagta
    22321 ttgtattttt tgtgctggct tggtggtttt caagaaaacc gacaaagata ttagattatg
    22381 ttggaaagtt cctgaatcct gcatttttgg ttttattagg tgctttgctt ctccttgctt
    22441 ttttgaatcc actaggttct gtaaatcaag cgcctattca gccaaattat caagagcatg
    22501 cttttttcac agggtttact caaggatata acacattaga tgcgcttgct gctttagcat
    22561 ttgggatcat cattgtcaca acaatccaaa acatgggtgt aacaaaacct gctgaaatcg
    22621 ctaaagacac gatcaaatct ggtgcgatca gcattatatt gatgggaatc atttatactt
    22681 tgctcgctta tttaggtgct atgagcttag gtagctttgc tctaagtgaa aatggcggga
    22741 tcacgctggc tcagattgct gatcattatt taggtactta tggcagtatc ttattagcat
    22801 ttatcgttat attagcttgc ttgaagaccg gtattggttt gattacagct tttgcagaaa
    22861 cattcacaga tttgtttccg caaagaaatt acgcattctt tgttgcactt gctagtatcc
    22921 ttccttgttt agcggcaaac gtaggcttga cgaatatcat tcaattttcg ttgcctgttt
    22981 tgatgttcat ctatccatta gcaatgacac tgattttact agtgttagtt ggtcctttgt
    23041 ttaagcagcg tacggctgtt tatcgaatga ctacttattt tacattgatt gcttcgattt
    23101 ttgatgggtt gaacgcttgc ccagaaacta tcaaacaaac acctattgtt caaaatcttt
    23161 tatatgttgc ggaaagctac ttgccgtttt tcaaattggg gatggggtgg atcgtaccag
    23221 cagttatcgg ttttgttatc ggactgattt ggagtttcgc taagaaagaa gaagtagcag
    23281 attgataaaa gaggttgtga aagagacgtt cagccttatt gccgataggc tgtctcttga
    23341 acactgttta ttgagaggtc gtgaaaaagc cgctcagttt cgagtattaa gaaatatttt
    23401 ttgaaaatgg cttctcacat ttttgcataa atttgactta ataccgagaa actggctttt
    23461 gaacaccgtt tattgagcgt ctataaactg acctgagtat tagagaaaca aaacggcaaa
    23521 gtagctccat atactttgcc gttttgtttt gaactaatac cgaagagaag ctgatcaaac
    23581 aacatttctt ttataatata gattttagac atatatatga tataaaaata gttttcgcaa
    23641 aaaaa
//
LOCUS       DAZKNH010000036        22881 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000036 DAZKNH010000000
VERSION     DAZKNH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.036"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..108
                     /locus_tag="ACG8MG_001341"
     CDS             <1..108
                     /locus_tag="ACG8MG_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0375396.1"
                     /translation="FTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQF"
     gene            212..358
                     /locus_tag="ACG8MG_001342"
     CDS             212..358
                     /locus_tag="ACG8MG_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFQ0375397.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            434..1681
                     /locus_tag="ACG8MG_001343"
     CDS             434..1681
                     /locus_tag="ACG8MG_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0375398.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            1696..2448
                     /locus_tag="ACG8MG_001344"
     CDS             1696..2448
                     /locus_tag="ACG8MG_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0375399.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(3151..3366)
                     /locus_tag="ACG8MG_001345"
     CDS             complement(3151..3366)
                     /locus_tag="ACG8MG_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFQ0375400.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(3385..3621)
                     /locus_tag="ACG8MG_001346"
     CDS             complement(3385..3621)
                     /locus_tag="ACG8MG_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFQ0375401.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            3879..6032
                     /locus_tag="ACG8MG_001347"
     CDS             3879..6032
                     /locus_tag="ACG8MG_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFQ0375402.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            6001..7416
                     /locus_tag="ACG8MG_001348"
     CDS             6001..7416
                     /locus_tag="ACG8MG_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFQ0375403.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            7449..8483
                     /gene="sppA"
                     /locus_tag="ACG8MG_001349"
     CDS             7449..8483
                     /gene="sppA"
                     /locus_tag="ACG8MG_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0375404.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            8485..9006
                     /locus_tag="ACG8MG_001350"
     CDS             8485..9006
                     /locus_tag="ACG8MG_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0375405.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            9003..9389
                     /locus_tag="ACG8MG_001351"
     CDS             9003..9389
                     /locus_tag="ACG8MG_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0375406.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(9885..9957)
                     /locus_tag="ACG8MG_001352"
     tRNA            complement(9885..9957)
                     /locus_tag="ACG8MG_001352"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9922..9924),aa:Lys,seq:ctt)
     gene            10338..10907
                     /locus_tag="ACG8MG_001353"
     CDS             10338..10907
                     /locus_tag="ACG8MG_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFQ0375407.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            10894..11754
                     /locus_tag="ACG8MG_001354"
     CDS             10894..11754
                     /locus_tag="ACG8MG_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0375408.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            11944..12648
                     /gene="yycF"
                     /locus_tag="ACG8MG_001355"
     CDS             11944..12648
                     /gene="yycF"
                     /locus_tag="ACG8MG_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFQ0375409.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            12653..14488
                     /gene="walK"
                     /locus_tag="ACG8MG_001356"
     CDS             12653..14488
                     /gene="walK"
                     /locus_tag="ACG8MG_001356"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFQ0375410.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            14485..15801
                     /gene="yycH"
                     /locus_tag="ACG8MG_001357"
     CDS             14485..15801
                     /gene="yycH"
                     /locus_tag="ACG8MG_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFQ0375411.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            15802..16671
                     /locus_tag="ACG8MG_001358"
     CDS             15802..16671
                     /locus_tag="ACG8MG_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFQ0375412.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            16726..17535
                     /locus_tag="ACG8MG_001359"
     CDS             16726..17535
                     /locus_tag="ACG8MG_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0375413.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(17638..18267)
                     /locus_tag="ACG8MG_001360"
     CDS             complement(17638..18267)
                     /locus_tag="ACG8MG_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0375414.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            18437..19729
                     /locus_tag="ACG8MG_001361"
     CDS             18437..19729
                     /locus_tag="ACG8MG_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0375415.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(19805..20467)
                     /locus_tag="ACG8MG_001362"
     CDS             complement(19805..20467)
                     /locus_tag="ACG8MG_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0375416.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            20692..20976
                     /gene="groES"
                     /locus_tag="ACG8MG_001363"
     CDS             20692..20976
                     /gene="groES"
                     /locus_tag="ACG8MG_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFQ0375417.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            21027..22652
                     /gene="groL"
                     /locus_tag="ACG8MG_001364"
     CDS             21027..22652
                     /gene="groL"
                     /locus_tag="ACG8MG_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFQ0375418.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         7841 a         3622 c         4549 g         6869 t
ORIGIN      
        1 ttcactgaat atcaagagga attaataaga aatttgagtg aaattggaag aactgctgga
       61 ataaatggtt tatatcgagc agattatgga gataaatctc aattttaatg atacttataa
      121 tattaaatat gtctcaaaat tttttgatta gaaaaatagg tatattgaat cagtgacata
      181 aaagtattta tagtttgtag ggggaatcgt aatggaagaa aaaaatagac taaatgctaa
      241 acaatgttca gatcaagagc ttaaaaaaat taaaggaggt gctggaacaa aacctcaagg
      301 aaaaccggca tcaaatttag tggaatgtgt tttttcatta tttaaaaagt gtaactaaaa
      361 ttttttttat gatatcctaa aatcaggagg ggatctcttg attactatcg atacaaaagc
      421 ttatctatta agaatgttcg tgggaaactt tttctttttt ctcttatttt atatgactaa
      481 tactgatctt aacaaaagaa aatatctttt tgtatgttta ttgataccac tcactttttt
      541 tgttgaatta tttactgatg tttccgcagc tatacctata atcggaagtt attttatatt
      601 aaaggaaaag aagaaaacta atatagtgct tttaaataaa ttactattat gtatgcttat
      661 tgatgatttc agatctatta ttacttccat ggttatgacg tatatgtttt catttgaagg
      721 gataaaaggg tatggttatg tagtattcca gataatgctt gattctttgt ttttgctgat
      781 gtttatttgg ttatatagaa aattagctat aaataggatt gttgaacagt ccagttctaa
      841 acttactgct atattcatga tttatttact tacaattggt ttatttgttt cttatgttgc
      901 tcatcaatat caagtatttg atcattttgt gtttggtgtc ttaatatttt taatcattca
      961 aatgtttgtt gttctatttt tatttattag aataactatt aggcaacgag aacaatatga
     1021 acagcaattg aaaagaaaag aattggttta tctaaaaaaa tatactgatt cactggaaaa
     1081 agatcaagaa aaatttgcca gatttagaca tgactataaa aatttattgc tgagtttaaa
     1141 agaaatcgct aatcaaaacc atgattcgat attggaaaaa gaaataaaag aattagagat
     1201 ctattctaat tcttatctaa ccaataagtt tgaatatcgt tatttaaaaa atataaaaaa
     1261 tgaatatttg aaaagtttgt tgatagcgaa gctataccaa gcaaataaca ataagatttg
     1321 ttgtaaattc gaatgtccaa gtactattat tgaagttcct gttccaatat ttgactgtat
     1381 tagggtgctt ggaattattc tcgataatgc aattgaagca tctatagaga gtcaagatag
     1441 agaactgtct ctagctattt accgagatga taaacagata gaaatttcga tatcgaatag
     1501 ttgtagagag atactactct ctatagatac tctaatgaaa aaaggaataa caacaaaaaa
     1561 gggacatcaa gggttaggtt tatctagtat agaggaaatc aataaaaaga atcccaatat
     1621 gtttgtgaac tatcaaaacg atccgtatca atttactact caagttattt tgttgttttg
     1681 agggaaggag gatttatgag ttatccaata atattatgtg aagatcaaat tatacaatta
     1741 aatcagttag aacgaattat tgataatttc attctttttc acgataaagt atttaagata
     1801 gtattgaaaa cacaaagtcc gttagaagtt aaaaaatacc tcaaacaatt ccgaccaaag
     1861 caaggaatat atttcttaga tattgattta aatcatgaag ttaacggtat agaattagca
     1921 gaagtaatca gaaaatatga tgttcaagca aaaatcattt ttacaactac tcatgatgag
     1981 atgttacccg taacaataaa aagaagagtt gaaacgttag gatttgtaac aaaagatcaa
     2041 acactagatg agtatcgaaa cgagattgtt gagttattgt tattagcgca agaaaggata
     2101 gatgcaacaa aacaggttac gaatcaagca tttgtttttt ccataggttc tcaaacattt
     2161 acttttgata taaatgacat ttattttgta gaagcttcaa aattaccaca tagattatca
     2221 ttgtgtacta aagatgggca gtacgaattt tatggaagaa ttagtgagct tgaaaagaaa
     2281 tatcctatgt taacaagaat tagtcgagcg tgtttagtaa atatctttaa cgttaaagaa
     2341 atagatttta aaaaacgttc actatatttt gattcagagt tggccagaaa ctttacttta
     2401 gggaaagcac aaaaaattaa ggagaagttg aaagaaagca ctgtttagtg gttttttctt
     2461 tagggagatt gcatttttct aataaaatag attccttcac tagtgcaaat ttttctgaat
     2521 cctattaaag ttgtaccaat ttttatagct gttattagtt acgtgcataa cagcaatatt
     2581 agttactgga ggaatatttc tatggtctgg aaccaaaaaa gggccaaatg aatagttctt
     2641 ttcttgtttt tattaagtac cataaagggg aattaaaatt aaatgtatgg tggaagatgc
     2701 gaaagatttg gcaaattcta ctaattttta gaaagtgttt ttcatttttt agttaaaaaa
     2761 tttgtcctgt caagtgcaat cctatgattg tacttgatag gatttgagat aattactttc
     2821 ctgtttttat aaatttaact tgcaggaaca ggctttaatg cacatccgac aagtacatta
     2881 ccaatacctc ctgtaacggc cgaaataggt cctcctgcag caccaactac agcgctgcaa
     2941 gtccttttgt aaaaatatct ctaccattac atttccaaag aatttttatc tgactttaat
     3001 aaaagataaa aatttaatat aattataaaa actagcacaa gaagctttag taaaaagttc
     3061 tgtccattcc aaacttttga aaagataaag aacataccta tgttcgtaat cgacacaaac
     3121 caatcttgag gtttgatatt ttttaattgt ttacgcaccg gtccacatgc tgaatccagc
     3181 acctaataaa gctccaccta caccacaagc agctattgca taaggtcctc cggctgaaca
     3241 aagttttact cctgctgttg ccccagaaaa agtagtgcct aaaactccca cggcactccc
     3301 accttgaata tttttcaact cttctgaaga taatataaat gttgtttttg tgtttttaat
     3361 atgcatacta cttattctcc ttttttattt ttttcgagca tcccagccag ctgcagcacc
     3421 tccagcgaca gctccatata accctccaac aacaccgcaa gctggtccgc aaattgctgt
     3481 tcctaaacct gcaccaccta ctgcccctgt ggtaagtcca cctacaactt gccccaattt
     3541 tccccaagca ttattgcctc caccaacagt ttgtgcattt ccacaatact tagttccttt
     3601 acattttgca tgtttaaaca cgcttcttta aataatattt tttacggtgc tactgtaaca
     3661 tacgtatttg attaacagta aaaatcttcc tgaaacttat caagtttatt cttgaatagt
     3721 aaaatttcca aaataaattt gattcaggaa tgaaaaagtt agtgttcaag aactgttttc
     3781 aaagtttttg tggaattatg agacaatatt ttcactgtat taaagctata ttgagaattt
     3841 ttctataaaa attttttaga aggagttgtg ttagataagt gataaaatca tttccttttg
     3901 tgcagcaaca agatgaaaaa gattgtggtg ttgcttgcct ttcgatgatt ttaaaatatt
     3961 ttaaaactga tatcccgatt cataaattac gggacctatc tgggactgat ttagagggta
     4021 cttcagcatt tggattgaaa aagacatttg aaaaattgtc ttttgattgt atggctattc
     4081 aagcagatag tgagatctgg aaagaaagag atcttatctt accattaatt gcacatatat
     4141 tggttgataa cagctatatg cactacgttg taatttataa aacgaaggga aacgtactat
     4201 ggattgcaga tcccgctaat ggaaaaaata gaaaaacgat tgaagcgttt caaaaagaat
     4261 ggacaggtat tcttttatta cctatgccta gagaaactta tattcctatt aaagaaaaag
     4321 tagctggatt aagttctttt ttgcctataa tttggaaaca aaaaggatta gtattccaca
     4381 tcgttttagc atcgattttc attacgtttt ttggcatagg aagctcatat tattttcaag
     4441 gtattcttga ttttttcatc cctaatcaag cacgttcaac tttaaatata atttccattg
     4501 gcttgatcgc tgtctattta tttcgggtat tgtttgaata tagtcgtagt tatcttctta
     4561 ttattttagg acagcgaatg agtatggcaa ttatgcttca gtatttcaaa catgtgttaa
     4621 ctttaccaat gaattttttt gctacgagaa aatcagggga aattatttct cgttttcttg
     4681 atgccagtaa gattattgat gctttagcaa gtgctacgtt atcaatattt ttagatattg
     4741 gtatggtgtt gcttgttggg gttacgttag ctattcagaa tagtacgttg tttttaatca
     4801 ctctatgctc tttgcctttt tatgtagttg caatattcgc tttcgtgaaa agctatgaaa
     4861 aatcgaacag ggaagaaatg gcagccggtg cagctgtaaa ttctagtatc atagaaagtc
     4921 ttaaaggaat agaaacaatt aaagcatata gcggtgaaga gaaagtatat aaccgtatag
     4981 accatgaatt tgttaaatta atgaaaaaat catttcgtac ggttacgctt gataatattc
     5041 aacaaggaat taaacatttg attcaattaa ttagcagtgc attaatttta tggctaggtt
     5101 cttattttgt tatggataat agaattagtc taggtcaact tatcacatac aatgctttac
     5161 tagtattttt gacagatcct ttacaaaaca ttattaattt acaagtgaaa atgcaaacgg
     5221 ctcaagttgc aaataatcgt ttaaatgaaa ttttcgcgat tgattcagaa aaatcgaacc
     5281 agtcttttga atatatgctt tctgaacaat tatttcaaca agatatcact gtaaaaaacg
     5341 tttcattctc gtatagcatg aaggccccaa cgttgaacga aatttcatgt acaattcctt
     5401 cgcacagtaa aacggctata gttggagtta gtggttctgg aaaatcaact cttgcaaagt
     5461 tatttgttcg tttttatgaa gcatcagaag gatcgatcca ttatggaaac attaatcact
     5521 tagacattcc ttttcagata ttacgcgatc gagtgacata tttgcctcaa gagtcatttt
     5581 tctttagtgg aacaatcatt gagaatctta tattcggttt agatagccca ccaagtttcg
     5641 aacaaataat agaagtttgt gagatagtcc aactaaatga atttattaat caacagccat
     5701 tacgttacga gacattgttg gaagaaggtg gaaataatct ttcaggaggg caacgacaac
     5761 gattagcctt agcacgggct ttattaaaaa atccggacat attaatttta gatgaagcaa
     5821 ctagtggatt agataccttg ttagaacatg caattatgaa ttatctcctc aatttaaaag
     5881 aaaaaacgat tatttttatt gcgcatcatt taccaatagc aaaagcatgt gatcaaattt
     5941 tagttctcca tgaagggcat ttggtagaaa aaggaacgca cgatgaactt cgttttgccg
     6001 atggtatcta tcaaaaatta tgggaaatct aggagaggga aacaatttat gcagaataat
     6061 aattggttcg aacattctag tatctatact aaacaacatc atagcttcta ccgttgggtg
     6121 ttataccctg taattctttt tttatgtatt attggattgt ttttaacctt tgctaaaaaa
     6181 gaggttgtta ttcggacaac ggctcaattg aaccccgaaa aaatagagaa gttacaagtg
     6241 ccattagaag ctaaaataat ggaaaacttc ctaaaagaaa atcaatttgt tcataaggac
     6301 gatgtgttgg ttagattgga ttgttcttta atagagaatg aaaaggctca gatagaacaa
     6361 gaaaaccaac gtattactca acaaataaag atggctcagc tatttattga aagtataagt
     6421 aaaggaaaaa atttgttttc aacggatgac agttttggct acagtaatca attaaagagc
     6481 atgttgtcag aaaaagaatc actccgctac gctttgaagc aaagtgaatt aaatgatcaa
     6541 aagcaattag aagtatacga aaagacaaaa agacaactag aaaaacaaat tgagagttca
     6601 gatagtaaat tacaagaatg gcaacaagta caggtagctt ggagtaataa tcaatcatta
     6661 aaagattttt caaaagaaat gatggcaaac tatgagaatt ggcaagaaca actaaataat
     6721 gtttctgatg atcaaaaaaa tcaagtgaaa ctgacaattt cagcaagcat aaatgaacaa
     6781 attgagcaac taaaaaaaga agtagaacag tatcagtcag aaaaagctaa attagttaaa
     6841 ccaactactt ctgagaatga cagaattagt caaacggaaa aaggaaagca agagctagaa
     6901 caagcaataa caactgtaaa acaaacagtt acagagctaa atgaaaaaca agagaagaac
     6961 cagtcagtta tcaaaagttt ggacgatcaa ctttcaaaag gaatactaaa agctcctgta
     7021 tctggaacgg ttcatttaaa tgaagaaaca aagggacaag cagaactagc caaaggaaca
     7081 gtgcttgcag aaatttatcc gatccacgaa aaatcaaaaa tgaaatttac agcactttta
     7141 cctgcgaatg aaagtaccta tattaaagaa gggatgaagg ttcattttaa attggatcaa
     7201 aaaggtgggg ctccgattac gatcgacgga actttagatg aaatttcaga aaattctact
     7261 gccactgaga aaggtgtttt ttatgcagtg aaaggaacgt taaaaacaac tcaatcgttt
     7321 ccgtttcgtt atggattaac tggagagttg tctttaattg taggagaaaa aacatattgg
     7381 caacaaattg tcgatgtgtt tctaaataaa aaataattat ttttactaag aatataggag
     7441 gagtttatat gaataaaaga agatggattg ctgtgggagt agcgtgcgga ttattgttac
     7501 tatccattat cgtcgcgcta attccaggaa aagataaaga ggaagcatca aatacaaccc
     7561 taacaggaat aaataagatt ttttacggat ctaacgaaat cacagaagaa acgttagaag
     7621 aaggcgcatc aaataaaaag attgtcaaac tatccgtcaa tggagttatt gcagatacag
     7681 gtgaaagcaa tctttttagt agggaacaat ataaccatca gaatttttta acacagctaa
     7741 agaaaattca agaagacaaa gcggttaagg gagtgttatt agaagtaaat tcacctgggg
     7801 gaggaatata tgaaagtgct gagattgcta aagaaatggc aaaaattaaa aagctagata
     7861 ttcctatcta tactgcattt aaaaatacag cggcaagtgg agggtactac atttctgctg
     7921 gttcagataa aatatttgct acggaagaaa caacaactgg atcaattggt gtcattattt
     7981 ctggtttaaa ttattctggc ttattagaaa aacttggcgt aactgatgcg acgtataaaa
     8041 gtggtgcatt aaaagatatg atgtcacctc aacacaaacc ttcagaggaa gagaataaag
     8101 ttatacagga atttgtgatg agtgcttatg atcgttttgt caatgttgta gcaaaaggaa
     8161 gaaatatgga tactaatgca gtgaaagaac ttgcggatgg aagaatttat gatggtaacc
     8221 aagcagtaga aaatgggttg gtggatcaaa ttggttactc tgaagatgca ttagattccc
     8281 taaaaaaaga gaagaaatta actgatgcga cggttataga atataaaaat gacacaactg
     8341 gctttgcttc ttcttggtta ggtaataaga ttgctgaatg gcaagggcta aaggcaacta
     8401 catcagatcg tgtaatttca gtttttgaaa aacttggatt ttctgatact ccaaaaccaa
     8461 tgtattacta tggagggcta taaaatgaat aagaagagtg gcattgaaac tttgcctaat
     8521 tatgtatttg cgggattttg gttacgattt tttgcttttc ttattgattt aatttgtatt
     8581 agcatcatca cccatgctac tataggtctc ttttacaaaa tcatgggact ggaatctgac
     8641 aaatccatta ttacttttta tggtatagcg tcattagcta tttatttaac ctattttgta
     8701 cttttgacta agctgaattc aggacaaaca attgggaaaa tgatttttgg gataagagta
     8761 gtgagctttg aggaaaaaga gctaagttgg caaactatct ttattcgtga gtgtatatgt
     8821 aggtttatat taaaaaattt tgtatttcaa attgggtatt tggtggctgc tttcacgccc
     8881 aagaaacaac atattgggga ttatttttct aacacgtcag tggttgttat tcgttcatta
     8941 gaagctatta actatggtca aaaaatttct aattctaaaa cacttaatct ggagagaatt
     9001 aaatgaataa gagaaaaaca gagtttattt tgattttaat tagtggaata acagggattt
     9061 tatgtagtct tttagcattg atgggtggta tgtttctatc ttctattgat tcaactactt
     9121 taaatactac agaactaacc agtaaggata tagatatgtt agtcaatcta agcgggaatg
     9181 ttgttttatt tggaggaatt accctagttt tttcgataat gattttcata ataggttttt
     9241 tagtaagaaa aaatcaaaaa acagtggtct atggtattag tttactcatt ttatctatta
     9301 ttccattttt tcttttaaca ttattgtggg taattcctgg agttttaggt attattgcgg
     9361 gattgatgct tctaataaga aaatcttaat taggcaatgt gtagacaacg tgaaacgatt
     9421 aatcaattaa aagctaaata cgttttgaga aatgataact tgtagtcgat ggaagacatg
     9481 tagataaaaa aactacgtgt cttttattta ttctaatttt ttcgaaaaaa agacactctc
     9541 tgattgttgt attaatggaa aaggggaaag cagcaagtaa atttagtttt tctattaaat
     9601 attcttcact ttttaacctt ttatagtact tagatttaat cctatatgtt tagagagttc
     9661 attggcagca tgattttgca aagcttctcg aattattgtt atattagaaa ctttactgtt
     9721 cataattatg cttctttttt aactcaaagt ccaaagggct acagtggact ttgagttaaa
     9781 aataaaaagc cctagttatt ttgaaaaata aaaaaagaac agcgtaaatg ttttcgaaat
     9841 gctttcgcac caacatttac ctgttccgtg ggtttggcac ccaatgggcc gtgaggggct
     9901 cgaacccgcg acccgctgat taagagtcag ctgctctacc aactgagcta acggcccgta
     9961 caaaaagtac ttaataatag tagcactaag aattttaaaa tgcaagacta catttaaaga
    10021 aaatcagaaa aatctttact tccttatagg aaatcattca tagattattc acatttgctc
    10081 gctattatat aagcatacca acaaacaaag ttcttgcaag agaacaaacc ctaacacttt
    10141 tttcattttt tactcctttc ccgccgtctc cccggcgggt ctcctgttct taacgataaa
    10201 agacaagaag gcttagcctt cttgtttttt gttttcatgg aatcccgcta atcaaatcac
    10261 ctagttaaag caactagatt gaatgtttat cataagtctg atatcatgtt tatgaaatga
    10321 aaaaaggagc ggttatgatg gaaaaaatgc gtcagcaatt aatggagtgg gggaaggaac
    10381 tagaaacatt ccgtctgcct cactgggaag aactccctga cttagaactt tatatggacc
    10441 aagtcagaac gttagttgat cgttatcttt cacctgtgat acaaggagaa aagcatccat
    10501 tgcttacctc ttctatggta aacaattacg ttaaactagg tttgatacca gcaccagtta
    10561 aaaaaagata taacaaagaa cacgtagcat ttttacttgc aatcactacg ttgaaacaag
    10621 tattgactat tcctgaaatc aaagaaggga tattgttcca aggaaaaaca gtaggaattc
    10681 gtgaagccta taatctattt tgcgatgaac aagaagcagc tgtgtggatg gtcagccaat
    10741 tagcacaagg taaatctcat ccgcaaaaat tcgaccaggc aacccctgta gaatatattg
    10801 ctgtaagagc ggcaacttta tcgtttgcaa tgaaacttct tgcggaaaaa acgattgttc
    10861 tagaaaccga atatttaaaa gaggagaaaa caaatgaaaa acaataaaat cgcaatttta
    10921 gtagattcag gtacggatgt tccagaagaa ttgataaaaa aatacgagat gtatatgatc
    10981 ccattgaaaa ttatttataa agatcaggta tatacagata aaattgacat tacccctgaa
    11041 gagatctatc aacgtttgcc agaggaaatc cctggtacat ctttgccgga tggtgagtcg
    11101 atcacagaaa tctttgagca aatcaaaaaa gacggttatg aaaaggtact tgcggtaaca
    11161 atttccagtg gattgagcgg gacatataat gtggttcgtt taattgcaca acagcaagaa
    11221 gaattggaag catatgtact agatacgaaa aatattggta ttggtgcagg tttttctgcg
    11281 atccaagcag caaaatggct ggaagaagga gtagaatggg atcaactgat cagtaatctg
    11341 aatgagttag tcgaaagaac aaaagttttc tttaatgttg cgacattgga atatcttcaa
    11401 aaaggtggac ggatcggttt agttgcttct attgtaggaa ccgctttaaa gcttaaccca
    11461 atcatctcat gtaatgaaga agggatttat tacacagtag gaaaagctag gggacgtaaa
    11521 aaaagtttgg ataaaacggt tgcttacgtc aaagaaaaaa tcggtaatgc aaaggtcttc
    11581 aacttagctg ttgcccatgg ggatgcgaaa gaagaagcaa ttgaaatgga agcgagactt
    11641 aaaagagagt tcccacaagc aaaccaaatc tactttggcc agatttctcc ggcattagta
    11701 gtccacactg gtccgggatt attgggtgtc ggtgtacaag ttttagaagg ataaatcaaa
    11761 aaaaggaaac actgaggtga aaatcagtgt tttccttttt tattgaagtt agaacaaaaa
    11821 ataataaagt attttgcact ttcattttaa ttttctcttt tgttttatag gcaaagatga
    11881 aacaaaagca gaaatacggt ataatgagaa ggtatgacaa taatatataa gaggtggctc
    11941 tttatgaaaa aagttttagt tgtcgatgat gagaagccga tttcggatat cgtcaaattc
    12001 aatttagcca aagaaggcta tgacgtttat actgcatatg atggggaaga agccttggaa
    12061 aaagttgctg aggtagaacc tgatttgatt ttacttgatt tgatgctacc taaaatggac
    12121 ggcttagaag ttgctcgtga ggtacgcaaa acttatgata tgccgatcat tatggtaaca
    12181 gccaaagatt ctgaaattga taaagtccta ggcttagagt taggcgccga tgattacgtg
    12241 accaagccat tttccaaccg agaattagtt gcacgtgtca aagcaaatct tcgtcgcggt
    12301 gccacggctg cgaaggaacc agaagaggca gcacctgcgg aattgacgat tggtgatttg
    12361 acgatccatc cagaagctta catggtgacg aaacgaggag aaacaatcga gttaacacat
    12421 cgtgaattcg aactactctt ttacttggcg aagcaccttg gacaagtgat gacaagagaa
    12481 catttattac aaacggtttg gggttatgat tattttggag atgtacgaac agtggatgta
    12541 actgtacgtc gtttgcgtga aaagatcgaa gataatccaa gccatccaaa ttatttagtg
    12601 acacgtcgtg gtgtcggtta ctatttgcgt aacccagaac aggagtaatt ggatgaaaag
    12661 aaaagtccgc ttttttcgat cagtcaattt taagatcgct attaccttta ttctgatttt
    12721 attgatctcg atcgaaatta ttggtgccta ctttatacgt ggtttagaac gttccacgat
    12781 caatacattt attaaagata tgaatcaaac ggtggaatca ttagcaacaa caatcagccc
    12841 ggaattgaat cgaaaagata atgcagatga tgaagaggta aatgcgaata tcaaaagatt
    12901 tattgaaaac agtgcgacta gcgatatcat cgaaatacgg gtcgttgacg aaaaagggat
    12961 catccgagga acaaccgatg tcaatgaaca aagcgctgtg ggaaagaaaa atgattatgt
    13021 tgatatcaat gatttcacga ctaaacgtta tgttgcttta gataacgacg ataaacgggt
    13081 gaatatcaat gttcaaccga tcctgtcccc gaccggcgat gccgttatcg gggctcttta
    13141 cgtcaaaagt aatatcgaac aaaaatattc ggaaatcaat aacacagcag tcattttctt
    13201 tactgcctca ctaattgcgg catttatttc aatgatcgta tctgttttag ttgctcgttc
    13261 gattacacag ccgatcggtg aaatgagaga acaagccatt cgaatcgcta gaggagacta
    13321 cagtcggaaa gtcaaagtgc atggacaaga tgaacttgga cagctggctg ataccttcaa
    13381 tcagttagct gaacgtattg aagaagctca agatacaatg gaagctgaac gcaatcgttt
    13441 ggatagcgtg ctatcccata tgacagacgg cgtcattgcg acagatcgca ggggtaaagt
    13501 cattacgatc aatgatatgg ctgtttcctt gctagatatc aaaaacgagg aagcaatcgg
    13561 acaatcgatt ttgacacttt tagatatcga agaagaatat acgttgcgga aattattaga
    13621 agatccaaac gaaatggtac ttgaccgttc aaccagtaca ttggaaagtg atcagatgat
    13681 cttgcgagta gacttttcta tgatccgtcg cgaatcaggt tttatcagcg gattagtagc
    13741 tgtcttgcat gatgtgacag aacaagaaaa aacagaacgg gaacgtcgtg aattcgtctc
    13801 taatgtttcc catgaacttc gtacaccgtt gacaagtatg cgtagttata tcgaagcatt
    13861 gagcgaaggt gcgtggaaag acgaggaagt agcaccaaat tttttgaagg taacgctaga
    13921 tgaaacagac cgaatgattc gaatgatcaa tgacctctta aatctatctc gcatggatac
    13981 aggtaatacc cagcttcaat tagaatatgt caatttcaac gagttagtga attttgttct
    14041 tgaccggttt gatatgatgg tgggaaatca ggaaaaaaat tataagatcc gtcgtgaatt
    14101 tacgcaaaga gatctttggg tcgaagtaga tacagataaa atcatccaag tagtcgataa
    14161 tatcatgaat aacgcgatca agtattcacc agatggggga acgattactt gtcgcttatt
    14221 agaaacgcat aataacgtga ttttaagtat caccgatcaa ggattgggga ttcctaaaaa
    14281 agatctgaac cgagtgttcg agcggttcta tcgagtagat aaagcacgtg caagagcaca
    14341 aggtgggaca ggacttggat tagctatttc tagagaagtg attaaggcgc atcgaggcgc
    14401 tatttgggca gagagcaagg aaggaaaagg gtcgactttt tatatctcgc tgccatatga
    14461 accttatgag gaggaatggt gggaatgaga atctctgaaa aagtcgttcg tataggactc
    14521 attttgatgg ttgcgttaag catatacttc tcttatgcaa tctggctgag cccagcagga
    14581 aaaagttcaa tcaacgtaga tgagacaaac actcaaatga tcgagtctca aagcaatcga
    14641 aaaacttccg aagtctttct tccgcttcat gttacatggc accattcggg aatgatccaa
    14701 gaaaccaaca gtgaaaatct cgtgtaccga ttgcaattga tcatcgataa agcaagattt
    14761 ggaaagcttt ctgaagtagt ttcaggagac aaatcacagt ttgaacaggt gaaaaatctg
    14821 aactctggtt ttgagttggc ctataatgcg ccttttttgc taagagaata caaggaagca
    14881 tttgatttag atttagatgt gtcgaacttg gcaaaagaaa gcgaaaatat gtattttact
    14941 agaatacaat tcgatcaagg aaataaaaaa atccgatttt tgaattattc gaaccatctc
    15001 gtatatgaag caaatatttc gttgaattgg acagatatca ataaagaatt aacagcaaaa
    15061 gacacaaaat ggacagaaat gacaacagaa ccatcgacga tcgacacgca atatgctacg
    15121 aaacattcga tcaaactgaa gaagtatagt tatatcctgt cacagcaacc ctatacactt
    15181 tttagaaatg cttttttcca gaaaccagat agcgtgaaaa ataatgatga aagcaatgaa
    15241 ttgatttttt acggtgggaa tgaaacaatg tctatccatt cggaaacgca agtagttgat
    15301 tttcgtggag agctgccaga gaaaaaagaa aatgctagtg tattttctga aagtttcccc
    15361 tatattagca aattaggcag ttctttaggg aacctcagat attttgaccg cagtgacaat
    15421 acgatcgatt atcggatatt cgtggaaggc ttcccggttt ttgggacaga cagcagagga
    15481 cagattggtg tggaagttgg cggtgaaacg aatggttctg tgcaagtgaa tatccaaacc
    15541 agtttgaata cgattcaagt gccgatacct tcagacgaag aagtagaact tgcaagtacg
    15601 aatgaaatca tccagcagtt aaaagctaag ggagcagata gcaacaagat tggttcagtg
    15661 atcgttggct atacatggca aaatgtcaaa gaaacgaatc gtgtcgttga tctcttacct
    15721 gaatggtatg tgaaatataa agataactgg tattcagcta acgaccttct ggaaaaatta
    15781 cctggattgg aggcgaatta attggatttc aaaaaaatcg aatggatatt tttcgtcgct
    15841 tttttagggt tgaatgtgtt tttattcagc agttatcatg acgcggtata ccaagaaaac
    15901 aatgtcattc gctctaatca gactgaatca atccaacaac gtctggctag cgacgatatc
    15961 acttacacgg ggaagatttc gtctgagaac aagcaaggct attatcttag cgcggagcag
    16021 accaatctta gcacttcatt agcgaattat cgcaaggctg gtaaaagcgg cttgctggca
    16081 agtggtgtgt ttttagacgg tgatgttttg acgaaatctt tatccagttc agatgctgaa
    16141 aagaacgtga tcgatccaga taaaattagt agttccttgg aagatttttt atctaacaaa
    16201 gacaatattt tattcggtaa tgattatgtg tacacgaaaa acttttctca tttagaagaa
    16261 gaaccactag aaatccaagc aagccaagag tatgaaggca tcccattcaa tgatgataca
    16321 gcaaaaatcg ttctttcttt agaaaaaaac gataatgatt atagcatcac gaaatacatg
    16381 cagacgcatt tatctgatat cgagcagctg cgcgaaaaga cggaactgta tacggaagag
    16441 gaagcaatca atacattgta catcaataac aaaataccaa gaggatcaaa aattttatgg
    16501 cgtcagctag cttattcctg tattctgaaa gtccgggaaa aaaatgtgta tgttcctgtt
    16561 tggcatgtag cgatcgaaac aagcgataaa gtggtccaaa ttgagagcgt caacgctttc
    16621 agcaatacga tcgtgacaaa caatactgta ccaaaagtgg aagatcaata aaaaagcgag
    16681 tataatgaaa aaagtttaca aagtaagaag ggaagtagga gtcaaatggc tgaagaaaaa
    16741 ccagctttta aaatcagtat cctagcaagt ggaagttcgg gtaattcact ttatatcgaa
    16801 agtgaaaaaa aacggctttt ggtcgatgca ggacttagcg gtaaaaaaat cacttccttg
    16861 atggctcaaa ttgatcggac accggaccaa ttagatggaa tacttgtgac gcatgaacat
    16921 cgagatcata tacatggcgt gggtgtcttg gctcgtaagt atcatttgga tgtttttgcc
    16981 aatcagcaga cttgggaagc gatggaacca ctgattggaa atgtaccagt ggaacaaaaa
    17041 catctatttg agatggggaa agtcagaaca ttcggcgata tcgatattga gagtttcggc
    17101 gtctcccatg acgcagcggc tccgcaattt tatcgtttcc ataaagaaaa tcggtcattc
    17161 gtcatgttga cggacaccgg atattgcagt gattatatgc gaggtgtcat tgaaaatgcc
    17221 gatggttatt tgatggaaag taatcatgat cttgagatgt tacgcatggg accttatccg
    17281 tggaatttaa aacagcgtat tttaggagat agaggacatc tttccaacga agacggtgca
    17341 cttgtcatga ctgatgtgat cggtaatcgg accaaacgaa tctatcttgg ccatcttagc
    17401 aaggaaaaca atatgaaaga acttgctcat ttaacaatgg aaaatgtttt gaaagaaaaa
    17461 gattttggcg ttggacatga ttttgaaatc tatgatacag atccagatga agcaactgag
    17521 ctattttcga tttaaacagt tcaatcaata aaaatataaa aagcagaaga taaaatcaat
    17581 cagggttggt tttatcttct atttttattt ttttagcata ttaaaaaaat aaccgtatta
    17641 tcgactgact acactgatgt gctgcttttc atgtttctca tccagtatct catctactaa
    17701 tgcaatcgcg taatctgcca tgctgatcgt actgtcgcta gcagcgtttc tttgtaaata
    17761 atcatcactt aacaaataag tacctgtacg tttgccatgt ggatcaaata aagaagctgg
    17821 actgatataa gtccaatgta gatttttaga ttgtttcaat tttaacaatc cttcgtacat
    17881 attgaaagct gttggataga agggtgcatt tttaggtact tggtcaatca tacgagtcgt
    17941 cttttctgta tctaagaata acgaagaggc acccccaacg acaattaagc gtgtatcggt
    18001 tccttcaagc atctcaatca aatgggacaa agaagtttga tgcatttctt cttttccttt
    18061 gggagcgttg aaagcatcaa cgacaacatc aaaagatgca agatcagtag ttgtcaactc
    18121 aaataaatcc ttttgtataa acggaacatc tgatggcaat ttttctggat gacgcacgat
    18181 tgccgtcacg tctaaatctc tggacaatgc ttcgtctaaa ataaatgaac ctgcatggcc
    18241 agtagctcca atgagtgcaa ttttcataaa aaacctcctt ttttattcct gtccattgta
    18301 agcagttgct gaatattcgt caaagaatcc gatttttgaa aagctcagtg cgcattcctt
    18361 tcatggttcg tgatcattgg gtatagttaa attaagaaaa ctgattgata aaaatagaag
    18421 ttgaaggaga taagaaatga aagagtggtt agatcgtgcc tttttaccaa tgataacatc
    18481 tgttgtgaat cttagaccct taactgcttt gcgtaatgga atgacttaca cgatcccaat
    18541 aattattgtt ggctcgctgt ttttcttatt tgctcatttt cctgtggatt acatcgctca
    18601 gtggatagac aaaacaggat tttctatgta ttttacacaa gtataccagg ctacatttga
    18661 tgtgatagcc ttttggatcg tttttgccat tgcatacgct tatgcaaaag aagaagacat
    18721 cgatcgctta ccagtcggca taacagcctt gtctgcttat ctactgctta ttcagccact
    18781 aaatactcag atagatactg tctggacagg tgctgaaggg atactaggag cagttacaac
    18841 tgggctcatc atcggatggg gctatgcacg tttagtaaga cttgcgaaag ggtggaagca
    18901 aagagagaag atacctgaaa atgtttggca gtcttttact tcattgattc caatcattgt
    18961 tatagtcact tttacgctta tcggttcagc ggtttttcaa catttgttca gaatgactgt
    19021 tgttcaagga gtatggacat atgtccagct tcccattcaa tcgttcatag acacgttgtt
    19081 tggtgtgctt ttattaggtt tttttgttcc gtttttatgg ttatttggtc ttcatggatc
    19141 tgcgatggtc aatgggttgg ttagtcccgt cttgcaagcg aattcattag caaatgcaga
    19201 aatcttagcc tcaggacaag agttgacggt ggcaaatggc ggacatatcg ttactcagca
    19261 atttctcgat cagttcatga cggttacggg tgctggattg acgttagggg ctgtattttt
    19321 tatgacggtt tttgcgaaat cgaagaaata cagagaatta ggaaatcttg ctcttttacc
    19381 agcctttttc aacatcaatg aatcaattat tttttccaca ccgatcgtga tgaacccgat
    19441 gatggctgtt ccttttattt tcgcaccggt cgtttcagga ttgattacct attccgcttt
    19501 atattttgga ttcgttcctt tatttacagc agtccaagtc ccttggacaa caccgccgat
    19561 cctctcgggt ttcttagtag gcggagtgcc tgcagcgatt ttacaaggta tcgtgttgtc
    19621 aattagtttt ttcatttatt tcccattttt gaaaaaaata gattctgcaa actgcaaaaa
    19681 ggaaagacag acaaaaaatg atggagcagc agaaaagatg aacgactaaa aaacaaaaat
    19741 actcctgtcg ctttagattg ggaaaatcac caatctagga tgacaggagt atttatagtc
    19801 atgtttatac tagccctttt tggacagcta attgaacaag aacaactaat gtgttcattc
    19861 ctacgtgtgt gatcatcgat gtccagatcc gaccagttgc tttgtactct aggctgaaga
    19921 agaaacctag gaaaaagtag attagcagat gaccatcatt atgagcaaac gcaaacagca
    19981 aagagctgat cactgctccg acccaaaaat ttgaatatcg actgatgatc cccagtatac
    20041 tgcggcggaa tacgaactct tccatgatgg gtccagcaac agttgtcgcc aaaataaaag
    20101 cgggtgcttc catgatcatc tggatgatat tttgtgtatt ctcggaaggg gttgctttgc
    20161 caaacaatac agattctaac atgacagcga tgctttgcag aatcaatgcg acaaagattc
    20221 caaccaatcc ataaataaca atctttggag cagaagcttt ttttaccgct gattcgatcg
    20281 ctaagggttc acgttgctta aaataaaaaa tagctaggac aacggcgcct agtatataca
    20341 tgactgcagt tgctgtgatc aattcagacg tagattttac aagtcctatc gaagctagga
    20401 tcaagggtga gataaaaatt agaccatagc aaaaaatgct gaggaagcta tattttttta
    20461 aagacatgta attcctcctt ttaagggata acaagcatgc aaggcaagct tatatcgatt
    20521 gatttcaatt atatcataat ttcaataagc agctgtcaga ataaaatttc ttttctttga
    20581 tttaaaagct tgcaaaaaag agggagaatg agtattatta aaaatgtagg ttagcactcg
    20641 aaggttaaga gtgctaacag attaaaaaaa gagttatcgg agggatttat cgtgttaaaa
    20701 ccattaggtg atcgcgtcat tatcgaagtc gcaaaagaag aagaaaaaac tgtcggagga
    20761 atcgttttag cttctgctgc taaagagaaa ccccaaactg ggacagttgt tgcagtcggt
    20821 gaaggccgtt tgttagaaaa cggtgaaaaa gttcctgccg ctgtaaaagc aggcgaccaa
    20881 gttatgttcg aaaaatacgc cggtacagaa gtaaaatacg aaggaaaaga atatctgatc
    20941 gtcgcaggaa aagatatcat ggcgattgtt gagtaaattt ggaagataaa aaaataatca
    21001 aaaataatca atgaggtgaa tcaatcatgg caaaagaatt gaaatttgca gaagacgcac
    21061 gcgcagctat gttacgcggg gtagataaat tagcagatac agtgaaagta acattaggac
    21121 ctaaaggccg aaatgtcgta ctagaaaaat cttacggctc tccattgatc acaaatgatg
    21181 gtgtgacgat cgcaaaagaa atcgaattag aagatcattt tgaaaacatg ggtgcaaaac
    21241 ttgtatcaga agtagcatca aaaacaaatg atattgcagg agacggtaca acgacagcaa
    21301 ctgttctgac acaagcaatc gttcgcgaag gtttaaaaaa cgtaactgca ggtgctaatc
    21361 ctctaggcat tcgtcgcggg atcgaattgg caacaaaagc agcagtcgaa gaattgcata
    21421 atatctcaac tgttgttgat tcaaaagaag ccattgcaca agtagcagct gtttcttcgg
    21481 gttctgataa agttggacat ttgattgctg atgcaatgga aaaagttgga aacgacggtg
    21541 tcatcacaat cgaagaatca aaagggatcg aaacagaact agatgttgta gaaggtatgc
    21601 agtttgatcg tggttactta tcacaatata tggtaacaga taacgacaaa atggaagctg
    21661 ttctagaaaa cccatatatc ttgatcacag acaaaaaaat ctctaacatc caagatattc
    21721 tgccattgtt agaacaaatc ttgcaacaat cacgtccatt gttgattatt gctgacgatg
    21781 tagatggtga agcattgcca actcttgtat tgaacaaaat ccgtggaaca ttcaacgtag
    21841 tagctgtaaa agctcctggt ttcggcgatc gtcgtaaagc aatgcttgaa gatatcgcta
    21901 ttttgacagg tggtacagta attactgacg atctaggttt agaattgaaa gatgcaacaa
    21961 tcgaaaactt aggaaatgct tctaaagttg ttgtagataa agacaataca acgatcgtag
    22021 aaggttctgg agaaaaagaa gcaatcgaag ctcgtgtgca attgatcaaa aaccaaattg
    22081 ctgaaacaac ttctgatttt gaccgcgaaa aattacaaga acgcttagct aaattagcag
    22141 gtggtgtagc tgttgttaaa gttggtgcag ctactgaaac agaattgaaa gaattaaaat
    22201 tacgcattga agatgcattg aatgctacac gtgccgcagt agaagaagga atggtctcag
    22261 gtggtggtac agctctagtg aacgttatca gcaaagtttc tgctgtagaa gcagaaggag
    22321 acgtggcaac aggtatcaag atcgttgttc gtgctttaga agaaccaatt cgtcaaatcg
    22381 ctgaaaacgc tggttatgaa ggatcagtga ttgttgataa attgaagaac gtggaactag
    22441 gcactggttt caatgctgct actggagaat gggtaaacat ggttgaagca ggtatcgtag
    22501 acccaactaa agtaacacgt tctgccttgc aaaatgccgc ttccgtttct gccttgttat
    22561 taacgacaga agcagtcgtt gcagataaac cagaaccagc agcgccagca gctcctgcaa
    22621 tggatccatc aatgatgggc ggtatgatgt aagaaaaagc aacttgctta tctacaactg
    22681 atcattagtg aaagacagat aaaaagaggc tggaacagaa gcgtttaact ccaagaaata
    22741 agaagaaatt cacggaaatt gcttttcaaa tttttgtgaa tttcagctta tttccgaagg
    22801 agttgcttct gcttccgctg tttatacgtg tttagagcgt gagacaaaag tgttctttac
    22861 ttttgtctca cgctcttttt t
//
LOCUS       DAZKNH010000037        22738 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000037 DAZKNH010000000
VERSION     DAZKNH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.037"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..911
                     /locus_tag="ACG8MG_001365"
     CDS             99..911
                     /locus_tag="ACG8MG_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFQ0375419.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            908..1708
                     /locus_tag="ACG8MG_001366"
     CDS             908..1708
                     /locus_tag="ACG8MG_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFQ0375420.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1869..3137
                     /gene="rseP"
                     /locus_tag="ACG8MG_001367"
     CDS             1869..3137
                     /gene="rseP"
                     /locus_tag="ACG8MG_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFQ0375421.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3205..4914
                     /locus_tag="ACG8MG_001368"
     CDS             3205..4914
                     /locus_tag="ACG8MG_001368"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFQ0375422.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5126..9484
                     /locus_tag="ACG8MG_001369"
     CDS             5126..9484
                     /locus_tag="ACG8MG_001369"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFQ0375423.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9629..10102
                     /gene="rimP"
                     /locus_tag="ACG8MG_001370"
     CDS             9629..10102
                     /gene="rimP"
                     /locus_tag="ACG8MG_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFQ0375424.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10125..11300
                     /gene="nusA"
                     /locus_tag="ACG8MG_001371"
     CDS             10125..11300
                     /gene="nusA"
                     /locus_tag="ACG8MG_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFQ0375425.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11322..11615
                     /locus_tag="ACG8MG_001372"
     CDS             11322..11615
                     /locus_tag="ACG8MG_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFQ0375426.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11612..11923
                     /locus_tag="ACG8MG_001373"
     CDS             11612..11923
                     /locus_tag="ACG8MG_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFQ0375427.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11936..14242
                     /gene="infB"
                     /locus_tag="ACG8MG_001374"
     CDS             11936..14242
                     /gene="infB"
                     /locus_tag="ACG8MG_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFQ0375428.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14269..14616
                     /gene="rbfA"
                     /locus_tag="ACG8MG_001375"
     CDS             14269..14616
                     /gene="rbfA"
                     /locus_tag="ACG8MG_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFQ0375429.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14773..15229)
                     /locus_tag="ACG8MG_001376"
                     /pseudo
     CDS             complement(14773..15229)
                     /locus_tag="ACG8MG_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            15394..17756
                     /locus_tag="ACG8MG_001377"
                     /pseudo
     CDS             15394..17756
                     /locus_tag="ACG8MG_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            18124..19047
                     /gene="truB"
                     /locus_tag="ACG8MG_001378"
     CDS             18124..19047
                     /gene="truB"
                     /locus_tag="ACG8MG_001378"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFQ0375430.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            19051..19992
                     /gene="ribF"
                     /locus_tag="ACG8MG_001379"
     CDS             19051..19992
                     /gene="ribF"
                     /locus_tag="ACG8MG_001379"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFQ0375431.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20344..21129
                     /locus_tag="ACG8MG_001380"
     CDS             20344..21129
                     /locus_tag="ACG8MG_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0375432.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21142..21993
                     /locus_tag="ACG8MG_001381"
     CDS             21142..21993
                     /locus_tag="ACG8MG_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0375433.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22117..22569)
                     /locus_tag="ACG8MG_001382"
     CDS             complement(22117..22569)
                     /locus_tag="ACG8MG_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0375434.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         7723 a         3964 c         4964 g         6087 t
ORIGIN      
        1 tttttaaata aaaaaacaat gatttcatag gaaaaacagt tagatattgc tataatactt
       61 aagatagtgg gaatctttta agtataggag gaaaaagaat gttacgtttt tttccgcaaa
      121 aaaacaaata tattcaagaa aaaagcgaat ttcaatttaa taccgatgga ccaattcctc
      181 agcacattgc catcatcatg gacggcaatg gtcgttgggc gcaaaaccgt cgtttgccac
      241 gagttgctgg ccataaagaa ggcatggaaa cagtaaagaa agtcacaaaa aaagcatcaa
      301 ggcttggagt gaaagtgttg acgctttatg ccttttcgac tgaaaactgg aagcgaccaa
      361 aagatgaagt aagttttctc atgcaactac ctgtcgactt ctttgatacg ttcgtaccag
      421 aattgattaa agaaaatgtc aaagttcatg tcatgggtta tgaaaacgta ttgccagaac
      481 atacgcaaga tgctgtaaga cgtgcaattg agcaaacaaa aaacaatact ggaatggtat
      541 tgaattttgc tttgaattat ggaagcagag cagagatcgt gactgctgtc caagaaattg
      601 ccgaagaagt agcaaaagga gaaatacacg cagaagaaat cgatgatgaa ttgattgcta
      661 agcatttaat gacaggtttc ttgcctaaag agttacaaga tcctgagctg atgatccgta
      721 caagtggaga agagcgaatt agtaattttc tgttatggca gatcgcatat agtgagctgt
      781 attttacaaa agcgctttgg cctgattttg atggtgcgca tcttgaagaa gcaatcgcct
      841 cttatcaaaa tcgagaccgt cgattcggcg gtgtaaaaaa aacagaagat gagaaagggg
      901 atcagtcatg aaacaacgtg tggtcacagc agccgtggcg ctagtcttgt ttattccgat
      961 tatatggatc ggaggcatag cggttgagct tacagcagca atcttagcag taataggagt
     1021 ttacgagctg tttcggatga aaggattgac actcttaagt tttgaaggag ttttatccgc
     1081 cgttggtgca gtgttacttg ttttgccaaa agaaagatgg tttttctttc ttccagagaa
     1141 tgtaagtaca tttatgttat tttatcttat agttatgatt cttttaggaa catcagttat
     1201 ttctaaaaat acgtatacga ttgatgaagc aggttttcct gtgcttgtca gcttgtatgt
     1261 gggaataggt ttccagaatt ttgtcaatgc acgaacagaa agcttggctg ttttgatatt
     1321 tggtctattt attgtttggg caactgatat cggagcatat atggttggtc gacgttatgg
     1381 tcagcataag ttaagtcctg atatttcgcc caacaaaacg attgaaggtg cgctcggtgg
     1441 tattggtagt gctgttgtag tcgcatttct ttatttattg ctatacccag caaaagaact
     1501 atttaatcat ggtatgtttg tcatgcttct gatgacgatt attttttcag cagtcggaca
     1561 atttggtgat ttggtcgaat ctgcaatcaa acgtcattat gatgtgaaag actcgggaaa
     1621 tatcttacca ggccatggtg gaatcttaga ccgtttcgac agtttgctgt ttgtatttcc
     1681 tatcatgcat ttatttggca ttatttagta agtatacctg cgtttctttg tgaatttcat
     1741 aaaagacgca ggtttttatt tttcattata ttgatactat tggtgtcttt ggtatactat
     1801 ggtaaaatta tagtgtttta tcgttgtttt ataaaaaaca caaaaaacaa gtaaaggaga
     1861 aaaaagctat gaaaacgatt ctgacattta tcatcgtttt tggtatatta gtgattgttc
     1921 atgagtttgg tcatttcttc tttgcgaaac gatcaggaat cctcgttcgc gaatttgcta
     1981 tcggtatggg tccgaaaatt tatgggcatc aagcaaaaga cgggacaaca tatacgcttc
     2041 gcctattacc tattggcggt tatgttcgaa tggcaggcaa tggggatgat gaaacagaaa
     2101 tggcaccagg aatgcctctt tctcttttac taaattctga cggcatcgta gagaagataa
     2161 atttaagtaa gaaaatccaa ttgacaaatg ctatcccgat ggaattgagc cgttatgatt
     2221 tagaagatga gctgactatc actggttatg tgaatggaga cgaaacagaa gtagtaacct
     2281 accctgttga tcacgatgcg acgattattg aaaacgacgg aacagaaatc agaattgcgc
     2341 caaaagacgt acagttccaa tcggctaaat tgtggcagcg tatgctgacc aactttgctg
     2401 gacccatgaa taactttatt ttggcaatcc tgttattcat cattctggca tttatgcaag
     2461 gtggtgttca agtgacgaac actaatcgcg taggagagat catgcctaat ggagctgcgg
     2521 cagaagcagg cttaaaagaa aatgatgagg tagtcagtgt ggacggaaaa gaaatccatt
     2581 catggaatga tctgacaaca gtcattacga aaaatccgga taaaacatta gactttaaga
     2641 tcgaacgaga aggtcaggtg caatctgtcg acgtgacacc gaaatctgta gagtcaaatg
     2701 gcgaaaaagt tggccaactt ggtataaaag ccccaatgaa tacaggtttt atggataaga
     2761 tcattggagg gacgcgtcaa gctttcagcg gttcattgga aattttcaaa gccttaggat
     2821 cactatttac aggtttcagt ttagataaat taggcggacc tgtcatgatg taccaattgt
     2881 cttcagaagc agcaaatcaa ggtgtaacaa ctgttattag tttgatggcg cttctttcaa
     2941 tgaatctcgg aatcgtcaat ctgcttccga ttcctgcctt agatggcggg aaattagtct
     3001 tgaatatttt tgaagggatt cgcgggaaac cattaagtca agaaaaagaa ggaatattga
     3061 cattagctgg ctttggattt ttaatgttgt tgatggtgct agttacatgg aacgatattc
     3121 aacgattctt tttctagaaa aagttgacaa atagggtata ataacaaacg ataactattt
     3181 tgaggaaata aaggagtata tagaatgaga caatcaaaaa tgttgattcc aacattacgc
     3241 gaagttccaa atgatgcaga agtattaagc catcaaatct tgctaagagc tggatatatc
     3301 cgccaagtat cagcgggtat ttattcatat ctgccattag caaatcgtgt attggaaaaa
     3361 ctgaaacgca ttatgcggga agaatttgaa aaaatcggag cggtagaaat gctgatgcct
     3421 gccatcttgc cagcagaact ttgggaagaa tccgggcgtt acgaaacata tggaccaaac
     3481 ttgtatcgtt tcaaagaccg caatgatcga aaaatgatcc ttggaccaac tcacgaagaa
     3541 acttttactg atttgatgcg taatgaaatc aattcatata agaaattacc tttaaatctt
     3601 tatcaaatcc aacctaaata tcgtgacgaa aaacgacctc gcttcggttt gcttcgcgga
     3661 agagagttta tcatgaaaga tgcgtattct ttccatgcaa cggaagaaag tttggacgag
     3721 acatataaag attacgaaaa agcctacacc gaagtattca agcgttgtgg cttagaattt
     3781 cgctcgatta tcggagacgg tggcgcaatg ggcgggaaag actcaaaaga attcatggcg
     3841 atctcagaaa tcggggagga tacgatctgt tattcgactg aaagtgatta tgctgcaaac
     3901 ttggagatgg ctacaagcta ctatgtatca aaaaaatcgc atgaaacaca gcttgaattg
     3961 gagaaagtag ctacacctaa cgtaaaatca attgacgaag tagcagattt cttttctgtt
     4021 gaacctcaaa aaatcatcaa gtctgtattg ttcattgcgg atgaacaacc tgtccttgtt
     4081 ttggtacgcg gagaccatga agtcaatgac gtaaaactga aaaacttttt atctgctgac
     4141 tttttagaag aagcaacaga agaagaagca caaaaatatc taggtgctga gtttggttct
     4201 gtcggaccag tcggtgtttc agatgacgta aaagtatatg cagaccgtta tgtccaagat
     4261 ttagcgaatg ccattactgg agcgaacgaa acaggattcc actataaaaa tgtgaatcca
     4321 gaacgcgatt tcactgtact ttcttatgaa gatcttcgtt ttgtacaaga aggagatcct
     4381 tcaccagaca acaatggtgt tcttgccttt actcgaggaa tcgaaatcgg acatatcttc
     4441 aaaatcggca ctcgttacag tgaatctatg ggagcaactg tactagacga aaatggacga
     4501 gaaaaatttg tgattatggg ctgttatggt atcggtgtaa gtcgtttatt atcagcaatc
     4561 gtagaacaac attcagatga acaaggaatc aactggccaa aaggaattgc gccatttgat
     4621 ttgcatatcg tccaaatgaa tctaaaagac gaataccaaa caaacctgac gaatgaacta
     4681 gaagaagcga tgacaaaagc tggctatgaa gtactggttg atgacagaaa tgaacgcgca
     4741 ggagtgaaat tcgcggattc tgatttgatt ggctgtccaa tcagaatcac tgtcggtaaa
     4801 aaagcagtgg atggaatcgt agaagtgaaa atcaagaaaa ctggcgaaat ggttgaagta
     4861 cgtaaagaag aattggtgaa tactttacca atcctcttga accaaggcga ataataagcg
     4921 ttaaaacaca gaaaataaaa atagctgaat cagctgtttt tgacaatctg atgattcgct
     4981 tttaaaattg gaactgcgat ttgaatcgca gttccaattt tttaatgtaa aactcagaaa
     5041 aaacgagtat actaaaaggt actgaaaaag aagaggataa gatgcgatca attgcattgt
     5101 ttctcatcag aaagggcgta tatctttgtc agaaaaacga gatttatttg aaaaactgtt
     5161 ggaacaaatc caactagagg aagcagaaaa aaatcatcca ctgcttactg ctggtgaaat
     5221 ggatagtgta gtggtccatc gtaaatcgcg tttatgggaa tttacgctgc atttttccaa
     5281 aattttgcct attatgctat atcggtcatt gactcagcat ctgactatcg cttttaaaga
     5341 tattgcacaa gtcaaactga atatcttagc agatgaccag acattcgatg aacagctctt
     5401 gcaagattac tgggcccaag ctttggaaaa tcagcaatgc gatacaccac tagcacagca
     5461 agtattgaaa acgcaggtac ctgtgataaa agatcgtaaa gtcatcttgc caatcgacag
     5521 tacaggagct atttcttatt tgaagcaaca atacttgcca ttagtagaag agttgttcgt
     5581 cagttacgga ttcccaaaat ttcgtatcga accagaagtg gatgagcagc aagcggaacg
     5641 agtattgaaa ctttttgaag aacgcaaaca agaacaggca gaagccttta tgaagcaagc
     5701 agctgaaagt ctagttgtcc acgaacaaaa gaaaaaggaa agaaaagagc aatcgccagc
     5761 tcttgaagga cccatccaca tccagctagg acgaaatatt ccagccgatg aaccaacgac
     5821 accaatgatc agtatcgttg aagaagaacg aagagtcaca cttgaggggt acgtattcga
     5881 caaagaagtc agagaactac gttcaaaacg caagatcttg actttgaaaa tcacagatta
     5941 tacttcatca tttatcgtca aaaaattctc gaacggcgaa aaagatgaac aagtatttga
     6001 tgcgatcagt gtcggtagct ggttaaaggt acgaggaagt attcaagaag atacgtttgt
     6061 ccgtgatttg gtcatgaatg cgcaagatat catagaagtc aaacatacac ctcgcaaaga
     6121 ttatgctcct gaaggggaaa aacgagtaga gcttcatgtt catagcaata tgagtacgat
     6181 ggatgcaaca aacagcatct ctgatcttgt ggcacaagca gggaaatggg gacaccgagc
     6241 tatcgccatt acagaccacg gtggtgcgca agcctttccg gaagcacata gtgctggaaa
     6301 aaaagcaggt gtgaaaatct tatacggcgt ggaagcgaat gtcgtagatg atggtgtccc
     6361 aattgcttat aatgatgcac atgaagcatt gagtgaagcc acatatgtcg tatttgacgt
     6421 ggaaacgaca ggattgtctg cagtatatga cacgatcatc gagctagccg cagtcaaaat
     6481 gtataaagga aatgtgatcg aaagctttga tgaatttatc gatccaggac atcctttgtc
     6541 acgaacaacg gtagatctga ctggtatcac ggatggaatg gttcgaggat caaagtcgga
     6601 agaagaagta ttgcggatgt tccttgaatt ttcaaaagat acaattttgg tagcccacaa
     6661 cgcggctttt gatatggggt tcttgaatac tagctacgca aggtatggta ttcctgaagc
     6721 ggccaatcca gtcattgata cgttggaatt agctagatat ctgtatcctc aattcaaacg
     6781 tttcggatta ggtgtgttat ctaaaaaatt tggtgtcagc ttggaacagc atcaccgagc
     6841 aatctatgat gctgaagcaa ctggtcactt agcatggatc tttgtgaaag aggcaatgga
     6901 caatcataac atgctttatc atgatcagct aaatgagcat atcggagaag gagattctta
     6961 caaaagggct cgacctttcc atgtaacgat tttagcaaaa aatcaggcag gactgaaaga
     7021 tttgttcaaa ctgatttcaa tgtcgaatgt cgaatacttc gaacgcgtcc cacgaatacc
     7081 acgctcccaa ttgaaaaaga tgcgtgaaaa tctattgatc ggctcagctt gtgataaggg
     7141 agaaatattc gaagccatga tgcaaaaagg ggtagaagag gcaagaaacc gagcaaaatt
     7201 ctacgattat attgaagtta tgccaaaagc ggtctatgcg cctttgatcg agcaagaatt
     7261 ggtaaaaaat gaacatgatt tggaagaaat tatccaaaat ttggtagaaa tcggaaaaag
     7321 tttggataaa atcgttgttg caacaggtaa tgtacattat ctgaacgaag aagatgccat
     7381 ttaccgaaaa attttgatca attctatggg cggagcgaat ccgttgaatc gtcatagttt
     7441 gccggatgtc catttccgaa caaccgatga gatgctaact gcatttcact ttttagggga
     7501 agaaacagcg aaagagatcg tagtagaaaa tacgaataaa atcgcagata tctgcgaaga
     7561 agtgattcca gtcaaagatg aactttatac accgaagatt ccaggttcag aagatgaaat
     7621 atcagaattg agttacacaa aagcaaaaca aatgtacgga gatccgttac ctgagatcat
     7681 tcaaaaacga ttaaaaaaag agttgaattc tattaatggt aatggcttct cggttattta
     7741 tctgatcgcc caaaaactag tgcataaaag taatgaagat ggctatctag taggttcacg
     7801 tggctctgtt ggctctagct ttgttgcgac gatgacagga atcactgaag ttaatccgtt
     7861 agcccctcat tattattgtc cagagtgcca atattccgag ttttttgaag atggtacgta
     7921 tggttctgga tttgacatgc cagaaaaaca atgtccaaaa tgtggtgcac gcttaaacaa
     7981 agacggtcat gatattccat ttgagacatt tttaggattc catggagaca aagtgcccga
     8041 tattgatttg aacttctcag gtgattatca agcagaagcc cataactata cgaaagtatt
     8101 gtttggcgaa gactatgttt accgtgccgg aacgattggt acagtcgccg acaaaacagc
     8161 atatggctat gtaaaaggat acgaaaggga caataatctg cagtttcgaa gtgcagaagt
     8221 cgacagatta gcaaaaggcg ctactggagt caaacggaca accggccagc atccaggggg
     8281 gatcatcgtt attcccgatt atatggatgt ctatgatttt acgccgatcc aatatccagc
     8341 agacgatcaa aattcggaat ggaaaacgac tcattttgat tttcactcga ttcacgataa
     8401 tgtattgaaa ttagatattc tagggcatga tgacccaact gttattcgga tgcttcaaga
     8461 tttatcaggg atcgatccac aaacgatccc tacggatgac ccggaagtga tgcggatatt
     8521 tgccggtcct gaagtacttg gagtaagtca ggaacaaatt tattccaaaa cagggacatt
     8581 aggtattcca gaattcggca ctcgttttgt tcgagggatg ctagaagaaa cgcatcctac
     8641 aacttttgca gaactactgc aaatttccgg actatctcat ggtacagacg tatggttagg
     8701 aaatgcggaa gaattgattc gacgaggaga tgcgacactg gcggaagtaa tcggctgtcg
     8761 tgatgatatt atggtttatt tgatccatgc tggattagat agcgggatgg catttaaaat
     8821 catggaaact gttcgtaaag gacaatggaa caagatccct gatgaattgc gagaaaccta
     8881 tctatcggcg atgaaagaaa ataatgtgcc cgactggtat atcgattctt gttcaaaaat
     8941 caaatatatg tttccgaaag cccatgctgc agcttacgta ttgatggctc ttcgtgtcgc
     9001 ttatttcaag gtttatttcc ctattttgta ttactgtgca tacttttctg tacgtgcaga
     9061 cgactttgat ctagtctcca tgtgcaaagg aaaagatgct gtgaaacaag caatgaaaga
     9121 aatcacggac aaaggattag atgcttccgt aaaagaaaag aatcaactga cagtgctgga
     9181 gttagccaat gaaatgctgg aacgaggatt caaatttgga atgattgatt tgtataaatc
     9241 ggatgccgtt aactttgtta ttgaaggtga tacgttgatc gcaccattta gagctgtccc
     9301 aagtttggga accaacgtgg caaaacaaat cgttgaagca agaaaagacg gtccattttt
     9361 atccaaagaa gatcttgcca cccgcggtaa agtttcaaaa acattgatcg aatatatgaa
     9421 cgacaatggc gtattgaaag atctaccaga tgaaaatcaa ttatcgttat tcgatatgct
     9481 ataagcatct gttttcctgt ctgcttgtaa ttttaagcag agtatggtat agttcttata
     9541 ggtaatgata ctaactgtga gtgagcggat ttttcgctca ctctttttaa tggaattacg
     9601 cacttaattt tttaaggagg cgaaactttt gagtagcgtc gttgaaacag tcacagaaat
     9661 ggtgacaccg attttagatg aacaaaattt tgaacttgtc gaagtcgaat ttgtaaaaga
     9721 aggaaaaagc tggttcttac gagtatttat tgacaaagaa ggcggtatcg atatcgaaga
     9781 atgcgccttt gtcagtgaga aactgagtga aaaacttgat gcagcagatc cagatccgat
     9841 tcctcaagct tatttcctgg aggtatcctc cccgggagca gagcggccat tgaaaaaaga
     9901 aagcgactat gaaaaggcac ttggtgatta tgtccatgtc tccttatacc aaccagtaga
     9961 tggcgaaaaa caatatgaag gatttcttca atcatttgat gctgaacagc ttacattgaa
    10021 aatacgcata aaaacacgag aaaaagaatt gacttttgac cgaaaaaata ttgcaaaagc
    10081 tcgcttagct atccaatttt aatcacggag aggaatcaga aaaaatgagt aaagaaatgt
    10141 taaatgcgtt agacgcttta gaagctgaaa aagggatctc aaaagaaatc gtgatcgatg
    10201 ctttagaagc agcattggtt tctgcataca aacgtcacta tggccaagca caaaacgtcg
    10261 aagtggaatt tgatcagaaa aaaggaaaga tccatgtcta tgcggtaaaa gaagtaacgg
    10321 aagaagtgat ggattcacag ttggaagtct cattgaaaga tgcactttta atcaatccag
    10381 catatgagat cggtgatacg atccgttttg aagtaacacc aaaagatttt gggcggatcg
    10441 ctgcgcaaac ggctaaacaa gttattttac agcgtgtacg tgaagcagaa agaacgatta
    10501 tttataacga attcagtgct tatgaaaaag acatcatgca aggaatcgtc gaacgccagg
    10561 ataaacgtta tatttatgtc aatcttggta aaattgaggc agttctttct aaacaagatc
    10621 aaatgccaaa tgaattttat caaccacatg atcgaatcaa agtttatgtt tcccgagtag
    10681 aaaatacgtc taaaggtccg caagtatttg ttagccgcag ccatccagac ctattgcgcc
    10741 gcttatttga gcaagaagta ccagaagtct atgatggaat cgttgaaatt gtcagcatcg
    10801 cccgtgaagc tggcgatcgt tcaaaagtgg cagttcgctc aaatgatcca aatatcgatg
    10861 cagtcggtac atgtgtcggg ccaaagggac aacgagttca agcaatcgtc aatgaattaa
    10921 aaggcgaaaa catggatatc gtcgaatgga atgaagatcc agctgtattc attgcaaatg
    10981 cattgaatcc tgcacaagtc atggatgtga tcttcgatga gacaaatcca aaggcctgca
    11041 cagttgtcgt tccagactac caattatcgc tagcaatcgg taaacgcgga caaaacgcac
    11101 gtttagctgc taaattgact aaccataaaa tcgatatcaa atcagaatct gacatgacgg
    11161 aattttatga aaatcaagaa caacaaaaag aaacagaaga actgcatgat gaagcaatcg
    11221 ttcagtcaga tttaacagac gatgaatatg aaacaattgc attcaacaat gaaacggtcg
    11281 aagaaaaacc agaagcttaa ctttagtaag gaggcaaaga gatgaaacaa aggaaaattc
    11341 ctttacgcaa atccgtggtt tcaggtgaaa tgaaacctaa aaaagagatg gtccgaatca
    11401 cccgttcaaa agaaggggaa gtatcgatcg atccaactgg aaaaatgcct ggacgaggag
    11461 cgtatgtctc acttgagccg gaagaagtac aacaagcttg ggataaaaaa attctcgatc
    11521 gagtactcga aacaacatta acagatgagt tttatcaaga acttcttgac tatgtcactc
    11581 accaaaaagc acgtaaagag ttatttggaa aatgaataca gttaatcggc aaaaagccat
    11641 gaatctcatc ggtctagcga tgcgcgcggg aaaactaatc acgggtgaag aactgaccat
    11701 tgctgaaatc cgcaaacaaa aagcaaaatt ggtgtttgtg gcaaccgatg caagcgagaa
    11761 tacaagaaaa aagataaaag ataagagttc atattatgaa gttccttgct ttgagctatt
    11821 ttctgaagca gaaatcacgc agatgatcgg aaaacctcgc aaggtattcg gaataatgga
    11881 tgccggattt gcgaagaaga ccaaggagct aattgaaggt taggaaggtg attgcatggg
    11941 caacaaaaga atctatgaat tagcaaaaga actcaaaaaa tctagtaaag acgtcgtgga
    12001 aaaagcacaa caacttggca tcgatgtgaa aaaccatatg ggtgcgatta ctagcaatga
    12061 tgagaagaaa ttgcaacaag cgttcaaaaa cccaagcacg aaacaacaag ctgcacaaca
    12121 agcaagcaga aaaccagcca atcaacagcc gaacgatcaa cgtaaaaaag aaacaacgaa
    12181 caatcaacaa aaaaataaaa ataaccgcag ttatcaagat cgcggtcaag ggagcggtca
    12241 agtgaatcaa ggaaaaaaac caacaacaaa ccaaaataat acgggaaata ccagcagcca
    12301 aaatcgtcaa ggaacgagcc aaggcggcaa tcaaaaccgt actaacaata acaacaaacc
    12361 aaacaataac aatcgcggca aatttaataa taataaccgt aatcgttata ataaaaaagg
    12421 taaaaaaggc aaacagcaac aatcaactaa accagctgta ccaccacgca aattccgtga
    12481 attgccagat gtattagagt atactgaagg tatgaacgta gcagatattg cgaaaaaaat
    12541 tcatcgtgag ccagcagaaa tcattaaaaa attatttatg cttggtgtca tggtcaatca
    12601 aaaccaagca ctggataaag atacgattga actgttagcg acggactatg gcatggaacc
    12661 acaagaaaaa gtccaagttg acattgctga tatcgataaa ttcttcgagg cagaagaagt
    12721 cgatccagat aaattagtct cacgtccgcc agtcgtaacg attatgggac acgtagacca
    12781 tgggaaaaca actttattgg atacattacg ccattcacgt gtaacaagcg gcgaagccgg
    12841 cggaatcaca cagcatatcg gtgcctacca gatcgatatt gatggtaaac caatcacttt
    12901 cttggataca ccaggacatg cggcctttac aagtatgcgt gcacgtggag caagtatcac
    12961 agatatcacg atcttagttg ttgctgcaga tgatggcgtc atgccacaaa cagttgaagc
    13021 aatcaaccat gccaaagctg caggcgttcc aatcatcgtc gctgtaaata aaattgataa
    13081 accaggtgca aaccctcaac atgttatgca agaattaagt gagtatgagt taattcctga
    13141 agcatggggt ggagaaacga tctttgttga gatctcagcg aaattcggcc aaaacatcga
    13201 agaattgctg gaaatgatcc tgttagtcgc agaagtagaa gatttaaaag cagatccaac
    13261 acaacgcgca atcggaacag tgatcgaagc acgtcttgat aaaggtaaag gtcctgtgtc
    13321 aaccttactt gtccaagaag gaacacttca tgttggtgac ccaatcgttg tcggtaatac
    13381 ttacggacgt gtccgtgtaa tgacaaacga tcttggccgt cgcgataaag aagcaggacc
    13441 tgcaacgcca gtagaaatca ctggcttgaa tgatgtacct caagcaggtg accgtttcgt
    13501 agtcttcgaa gacgaaaaga cagcacgtgc agcgggtgaa gaacgcgcga aacgtgcatt
    13561 attagaaaac cgtgctgcca caagccgtgt gactttggat aacttatttg aaagcctgaa
    13621 agaaggcgaa ttgaaagaag ttaacgtaat catcaaagca gatgtacaag gttcagcaga
    13681 agcactggct gcatcattga agaagattga tgtggaaggt gtccgtgtaa aaatcgtcca
    13741 ttcagctgtc ggagcaatca atgaaagtga tgtaacctta gcagcagcca gcaacgctat
    13801 tatcatcggt ttcaacgttc gtcctacacc gcaggcaaaa gtgcaagctg atactgaaga
    13861 agtcgatatt cgtcttcacc gtatcatcta taaagccatc gaagaaatcg aaactgcgat
    13921 gaaagggatg ctagaccctg aatttgaaga aaaaatcacc ggtcaaatga tcgttcgtga
    13981 aaccttcaaa gtttctaaag ttggtacgat tgcaggtgcc tatgtaacag aaggatatat
    14041 tcgtagagat agcggtgtcc gtgtaatccg tgacggaatc gtaatctatg aaggccaact
    14101 tgcaagtctg aaacgattca aagatgacgt gaaagaagtc aaaatgggtt atgaatgtgg
    14161 tgcaatgatt gaaaaattca atgacatcaa agtagatgac gtaattgaag gatttatcat
    14221 ggaagaagtc aaaaatgact aaaaactgat aaaaggaggc caatcattat ggctaactac
    14281 cgtgaccgcc gagtcggtca agaaatctta aaagaagtca atgatatttt gcgcaaaaag
    14341 gtccgtgacc ctcgtgtgga gaacgtaacg atcacagatg tgaaagttac gggggactta
    14401 cagcaagcaa cgatctatta cagcatttta tctgacttag catcagataa gaaaacagca
    14461 caagatggat tgaataaagc atcaggattg attcgtcgag agttagggca caatctaagt
    14521 atttataaga cacctgaatt gttctttgaa cttgatgaat cagttgtcta tggcaaccat
    14581 atcgatgaat tgcttcgcga cttgaacaaa gactaatcaa aaactcccgc aaatcctgtg
    14641 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
    14701 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
    14761 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
    14821 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
    14881 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
    14941 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
    15001 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
    15061 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
    15121 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
    15181 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
    15241 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
    15301 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
    15361 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
    15421 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
    15481 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
    15541 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
    15601 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
    15661 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
    15721 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
    15781 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
    15841 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
    15901 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
    15961 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
    16021 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
    16081 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
    16141 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
    16201 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
    16261 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
    16321 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
    16381 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
    16441 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
    16501 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
    16561 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
    16621 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
    16681 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
    16741 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
    16801 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
    16861 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
    16921 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
    16981 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
    17041 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
    17101 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
    17161 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
    17221 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
    17281 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
    17341 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
    17401 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
    17461 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
    17521 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
    17581 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
    17641 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
    17701 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
    17761 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
    17821 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
    17881 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
    17941 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
    18001 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
    18061 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
    18121 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
    18181 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
    18241 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
    18301 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
    18361 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
    18421 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
    18481 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
    18541 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
    18601 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
    18661 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
    18721 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
    18781 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
    18841 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
    18901 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
    18961 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
    19021 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
    19081 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
    19141 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
    19201 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
    19261 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
    19321 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
    19381 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
    19441 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
    19501 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
    19561 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
    19621 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
    19681 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
    19741 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
    19801 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
    19861 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
    19921 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
    19981 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
    20041 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
    20101 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
    20161 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
    20221 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
    20281 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
    20341 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
    20401 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
    20461 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
    20521 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
    20581 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
    20641 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
    20701 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
    20761 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
    20821 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
    20881 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
    20941 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
    21001 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
    21061 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
    21121 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
    21181 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
    21241 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
    21301 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
    21361 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
    21421 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
    21481 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
    21541 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
    21601 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
    21661 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
    21721 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
    21781 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
    21841 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
    21901 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
    21961 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
    22021 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
    22081 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
    22141 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
    22201 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
    22261 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
    22321 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
    22381 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
    22441 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
    22501 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
    22561 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
    22621 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
    22681 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaa
//
LOCUS       DAZKNH010000038        22733 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000038 DAZKNH010000000
VERSION     DAZKNH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.038"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..1956)
                     /locus_tag="ACG8MG_001383"
     CDS             complement(25..1956)
                     /locus_tag="ACG8MG_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFQ0375435.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1974..2888)
                     /gene="pfkB"
                     /locus_tag="ACG8MG_001384"
     CDS             complement(1974..2888)
                     /gene="pfkB"
                     /locus_tag="ACG8MG_001384"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0375436.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2885..3637)
                     /locus_tag="ACG8MG_001385"
     CDS             complement(2885..3637)
                     /locus_tag="ACG8MG_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0375437.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3797..4006)
                     /locus_tag="ACG8MG_001386"
     CDS             complement(3797..4006)
                     /locus_tag="ACG8MG_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375438.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4017..5344)
                     /locus_tag="ACG8MG_001387"
                     /pseudo
     CDS             complement(4017..5344)
                     /locus_tag="ACG8MG_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5480..5635
                     /locus_tag="ACG8MG_001388"
     CDS             5480..5635
                     /locus_tag="ACG8MG_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFQ0375439.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5709..6296)
                     /gene="yihA"
                     /locus_tag="ACG8MG_001389"
     CDS             complement(5709..6296)
                     /gene="yihA"
                     /locus_tag="ACG8MG_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFQ0375440.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6379..7629)
                     /gene="clpX"
                     /locus_tag="ACG8MG_001390"
     CDS             complement(6379..7629)
                     /gene="clpX"
                     /locus_tag="ACG8MG_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFQ0375441.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7876..9165)
                     /gene="tig"
                     /locus_tag="ACG8MG_001391"
     CDS             complement(7876..9165)
                     /gene="tig"
                     /locus_tag="ACG8MG_001391"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFQ0375442.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9319..10251)
                     /locus_tag="ACG8MG_001392"
     CDS             complement(9319..10251)
                     /locus_tag="ACG8MG_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375443.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10408..11067)
                     /locus_tag="ACG8MG_001393"
     CDS             complement(10408..11067)
                     /locus_tag="ACG8MG_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0375444.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11070..12095)
                     /gene="sppA"
                     /locus_tag="ACG8MG_001394"
     CDS             complement(11070..12095)
                     /gene="sppA"
                     /locus_tag="ACG8MG_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0375445.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12108..12398)
                     /locus_tag="ACG8MG_001395"
     CDS             complement(12108..12398)
                     /locus_tag="ACG8MG_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375446.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12807..14612
                     /gene="glmS"
                     /locus_tag="ACG8MG_001396"
     CDS             12807..14612
                     /gene="glmS"
                     /locus_tag="ACG8MG_001396"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFQ0375447.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14684..16039)
                     /gene="glmM"
                     /locus_tag="ACG8MG_001397"
     CDS             complement(14684..16039)
                     /gene="glmM"
                     /locus_tag="ACG8MG_001397"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFQ0375448.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16063..17232)
                     /locus_tag="ACG8MG_001398"
     CDS             complement(16063..17232)
                     /locus_tag="ACG8MG_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFQ0375449.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17229..18116)
                     /gene="cdaA"
                     /locus_tag="ACG8MG_001399"
     CDS             complement(17229..18116)
                     /gene="cdaA"
                     /locus_tag="ACG8MG_001399"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFQ0375450.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18305..21997)
                     /gene="nifJ"
                     /locus_tag="ACG8MG_001400"
     CDS             complement(18305..21997)
                     /gene="nifJ"
                     /locus_tag="ACG8MG_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFQ0375451.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22196..22717
                     /locus_tag="ACG8MG_001401"
     CDS             22196..22717
                     /locus_tag="ACG8MG_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFQ0375452.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRCLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         6328 a         4939 c         4045 g         7421 t
ORIGIN      
        1 aacccagcct cttctttttt atttctatgc ttctgctact gcaggagctt ttctcaataa
       61 accataaatc acaccagata cgactgctcc gatagcaata aacaccaaat acatgaccgg
      121 gtgactcagt aagaatacaa cgaaaatccc gccgtgagga gccagtaatt tgattccgaa
      181 agctccgact aatccgccag ttaaagcaga accaataata aaacttggaa tggcacgaat
      241 cgggtcagct gctgcaaatg gaatcgatcc ttccgtgaca aacgatagtc ccatcacgat
      301 gttcgtcaaa ccagcatctt gatctgtttt gctgaattta ttcttaaaca aacgtgtagc
      361 aacgaaaatt gccaatggag gtaccattcc tccagccata acagaagcca tcacgacact
      421 tccgccagtt gcaacagtcg tagctaaagt tgctgtacca aacacataag cggctttgtt
      481 gattggaccg cctaaatctg ccgccatcat tccagctaat aatgcaccaa gtaaagcagc
      541 attcgaacca ctcatacttg tcaagaaacc gttcaatgca tcattgattg ctttcatagg
      601 gacattgacc agcaacatca agaaaccagt aatcagtaag ccaaacactg gataaaagag
      661 gatcgtttta attccatcca atgattttgg taagttcttg aataatttac gtaaaaatac
      721 gatgacataa ccagctaaga atccaccaat cagtgcacct aagaatcctg caccgcctgt
      781 attagctaat gcaccagcag caaatccaac gatcaaacca gggcggtcac cgatacttga
      841 agcaataaat cctgctaata ccggcagcat gaaaccaaat gcagcttgtc cgatttggtt
      901 gaaccagcta gcagcttgat tgtaattccc tagatttgat aattgatcat gtggcacgcc
      961 aataaattgg tcgatcatga aagaaagcgc aatagcgatt cctccaccaa taacgaaagg
     1021 taacatgtgt gatacgccgt tcatcaaatc tttgtaaatt ctttgtccga tagtaccatc
     1081 cgcatttcct tcttctgaac tgctttcttt tccatcacca tggaatactg gtgcagtacc
     1141 actaagggct agattgatca actcttctgg tttacggata ccatcactga caggacgatt
     1201 gatcagttct tttccgtcaa agcgattcat ctctactttt ttgtctgctg caacgattac
     1261 accttctgca cgggcgatgt cttcagcagt caatcggttt ttgattcctt ctgaaccgtt
     1321 cgtttcgact ttgatctcca cacccatttc tttcgctttt ttcttcaaag catcttctgc
     1381 catgtaagtg tgagcgatcc ctgttggaca agctgtaacg gcaacaacga attttctttc
     1441 tggatgagca gaagcttctg cagcttgtgt ttcttgttct gctttttctt ctgcttcttt
     1501 ttgctcttca gctgtttgga acaagtcttg gacttcttct ggtgtatttg cttcttttaa
     1561 ttttcctacg aaatctgcat caatcagcaa tcgagatagt gctgctaatg cttgcaaatg
     1621 tgtgtcgttt gcgccttctg gagctgcgat catgaaaaac agataagttg gctgtccgtc
     1681 taaggcttca taatctactc ctttttggct ttttgcgaat aaaactgttg cttctttaac
     1741 tgcactgttt ttcgcgtgag gcatggcgat tccatcacca agacctgtag aagtttgtgc
     1801 ttcgcgggct agaattcctt ctttatatgt ttcgatatcg gaaatccggc cggcatcaaa
     1861 cattttttgg accatttcat cgattgcgcc ttttttatca gtcgcttgca aatccatgat
     1921 catcgcttct ttgaccatta aatccttgat attcatgatt atcctccttg aaactaatca
     1981 atttttgtga tttcaacttc tggcagcaac tcatcaatca attcgcgagc tgccaagtca
     2041 tcagaaaaag ctgttgcgct tccgcaagca actccccatt taaaggcttc gattggatct
     2101 ttcgtacggc tgtaagcccc aataaagcca gcaatcattg aatcccctgc acctactgaa
     2161 tttttcaatg ggcgaactaa tacattcgtt ttataagttc cttgttccgt gaagagcaaa
     2221 gcaccgtctc cagccattga gatcagcaca ttctgtgccc cttcagctag taattttttc
     2281 ccatacggaa tgatttcttc tacagattgg aaggttgtgt tgtaaagctc agctaattcg
     2341 tgattgtttg gtttgatcaa caacggtttc ttaacaagtg cattttttag gtcatcacct
     2401 gttgtatcaa tcacgaaatc tgcacctttt tctcgaatga tatcaatcag ttcttgataa
     2461 aatccttttc gcaggctagc tggcgcgctt cctgacaata cgacgatatc tccttgtcct
     2521 actgttccta atgcttgctt caactcatcg atttcttcat ctgaaataga tgggcctaag
     2581 ccattgatct cagtttcagt gtctgatttt agtttgatat tgattcttgt atcttctttt
     2641 acggttgtga acaaagactg gatttgttct ttttcaagcc acttcgaaat aaattcgcca
     2701 gtgaaccctc ctaaaaaccc tagtgctgta gattgagaat cgatccgttt caaaatacgg
     2761 gaaacattga tcccttttcc tccaggtaac ttgaagtctt ccttcattcg gttcaaatcg
     2821 cctagtttta acccatctac gcgtacgata taatcaattg atggattaag tgttactgtg
     2881 taaatcattg tattgcctcc ttaattgttg tcttttcacg gaattcatta acgaattcta
     2941 atggacaacg ctcggttatg atcgtggctg cttccaaagg tgcaacagag gtgaaggaca
     3001 actggttgaa tttggtatga tcaatcaata caaatgtctc ttcggaattt ttcatggcta
     3061 aacgtttaac agctgcctct tctggatccg gagtcgtgaa tccttgttca agatgggcac
     3121 cgttcattcc cataaatgct ttattaaaat gaaaattttt tagttgttct atcgcattag
     3181 atcctaaaac agcattagtt gccagcttta tcgttccacc taaaataatg gtagcaagct
     3241 ccaaatcaac taaatttgcc gcatgttgaa cggaatttgt cactactgtg atctgttttc
     3301 cttgcagaaa aggaatcatt tctaacgtag tagagccagc gtctaaataa atgacatctt
     3361 cgtcattaat ggtttcagct gccgatttgg cgatttgctg tttttcgtga acgtttttga
     3421 gtgatttttc actcatattc tgctcaaaat tcaataattg ctctttctta gctccgccat
     3481 gaactctttc tagaaaccca ctattctcca gctcttgtaa atcacgtctt atcgttgatt
     3541 cagaagcatt caaaaggttt acaagatctt gtgatttcac gattttctgc tgttcaagca
     3601 agtctaggat tttttgtctg cgttcctctg taagcatttt cacgatcacc tcaatacaaa
     3661 taatagcata ttttttttct actagcaata taaatcagtc aaaaacgttc ataaaaatta
     3721 taaatcagtc aaaaaaaaaa taaaaaacca gtcagaaaga ctggtttttt atttttttgt
     3781 ctatcatcaa gccgttttat cgttttttat tttcgcttct tgtttttttc atataaacag
     3841 aagcaggttt tctttcctta tgaagaagat aataataaag tcccatcccg ccaaacatga
     3901 ccaaaaaacc gatgactgat cgaataggaa aattaggaac aaaaaagaga cagacggata
     3961 ataatgatgc cggaaccatt attcgataat agagttgttt atccattttc ttcacctcat
     4021 attttttgta aagtaatcaa ttgttctaat tctcggtaat ctaaaaattg cacacgcaga
     4081 tgctgctcac ctttcttgac atcgaattgg atacttcgcc gctttttctt tattcgtttg
     4141 aaggtaacat cagagagtct atgtttgatt tcctttaacg gtaattctga cagggattct
     4201 gaaggaacat agattcttaa ggataaaaga tactcttgat atttttcatc tgttacctct
     4261 acttctagat attccctaat ataataacaa taagcttcga ttccattatc caaagcaaca
     4321 gtcggatcga taactttata gcacatcatg cgtgatcgat tctcgtcaac ccaaggaaaa
     4381 tacgcctgag catttagtgt tccgcgaaca cgcctttctt tataatctcc ttgttggata
     4441 tcataacttt ttgttacaaa aaatgaagca ttctttttag gaacaggttg catttgtgca
     4501 ttctgatact ggacagattg gatcgtatgg tcagtgtaaa tgtattcaac gaatggcggt
     4561 ttcaataaac cgacaccatt gtcccaagca agatgatcaa acccaagctg gtccctaaaa
     4621 taggattgaa acatttgatt gtttggcttt tgccagttaa aggggtctcg ataaagctgg
     4681 atacatatga cttcgttttc taattccttc actttttcta cttgttggat gtcgagaaaa
     4741 atcggtttag ggatgatttg atgataatag cgactataat acatttccca acacgaagtg
     4801 aaacataaac cttggtaaaa aagatcgtat cctggaaatt ggttgcaatc gactactact
     4861 tccccgtttt gttgtttcat tttaggtaaa tgttcgattt catccttttt ctcaaacaat
     4921 agccgttgag agatctgttt tgtattgtga tagataaact cgctataagc tcgcatatag
     4981 gcgaacaccc catgagcaat catttttttc tgaatgaagt ctcgaatcaa aaattgcttt
     5041 tctttgaaaa cagagataga caatacattt tttatcaata attgattatc ttccaagcga
     5101 aaactacatt cgttctcgtc atttcctaca actaaccaac tttcgtctct taccgatgct
     5161 agcattattt tttcttgaag ctttcgaaga tcatacggat ccgcttgttg tgtgaaagag
     5221 cggataaatg ttggctggat caacagacgt gtcaaaatag cccattcgct tatttcctgt
     5281 ttttttcttc ttcatttaag tctgtgagct ggatagtcat caataattta tcttcgccaa
     5341 tcattttctc acctctttca aatcttttta taatttaatt ataaccaatg ataaagaaaa
     5401 actaaaactt tatgtcttag aaaccaattt ggacgattac atgatagact aagtaaaaag
     5461 aaacaaagag gtgtttacaa tggggttgaa atttgaacgt acagataatt tagataaact
     5521 tttcgaatca tttgctgtcg atccggaaaa aacagttgca ccaaaagaag agatcgatcg
     5581 atttctaaaa ccacagccac aaaatgaaaa gaaaaaagac aagaaacaaa agtaagttaa
     5641 gctcaaaaaa agacttacca gccaaacttt tatgaaagag tttgactggt aagtcttttt
     5701 tattcttttt atgattcaat tgcttgttcg attgctgccc acgcttcttc ttttccctgt
     5761 tttgtaacgg aagaaaagag aataaacgtg tcattgcgat caaaatccaa cgcttttttg
     5821 atttcgctct catgcttgtt ccatttacca cgagggatct tatctgcctt agtggcaaca
     5881 acaattacag ggatatcata atacttcaaa aattgataca tctggatatc ttcttgagaa
     5941 ggcttatgac gcaaatctac taaagaaacg accgcacgta gctggtctct agtcgttaaa
     6001 tatgtctcga tcatctttcc ccattttgct cgctcagttt tggatacttt tgcataccca
     6061 tatcccggaa catcgacaaa atgtaatgcg ttctcgatca aataaaaatt caatgtctgg
     6121 gtctttcctg gcttaccgga agtacgggct aaattctttc gatcaatcaa tgtgttgata
     6181 aaagaagatt tccctacatt tgatcgtcct gccagagcta tttctggcaa ttgtgtatct
     6241 ggatattgtt ttggtgccac tgcacttatg ataatttcag cttgatggac tttcatggtc
     6301 tctctcctta acaaggtgat gaataaagca agcggctgtc gcaaaagcca aaatgacttt
     6361 tacggcagcc tcttttcatc aacccgcttt tttctctgaa tctttataga ttactgttgg
     6421 ctcaccagac aattcagctg cttcttttgt gataatcact ttttcgatgt tttcatttga
     6481 agggatatca aacatcacat ccatcatgat ttcttcaatg atggaacgaa gtcctcgagc
     6541 accggtattg cgttcgattg cttttttcgc aatggctctc aacgcttcag gttcgaattc
     6601 aagttcggta ttatctagag aaagaagttt ttgatattgt ttgactagcg catttttcgg
     6661 ctcagtcaag atccgtacca gatcatctgt tgtcaatttc tctaaagctg ccataacagg
     6721 taatcgtccg ataaactcag ggatcaatcc gaattttagt aaatcttcag gaatgatatg
     6781 ctgcatgaca ctttcgtctt cagaaagttt ttggttttgt gtaccaaatc caatcgtctt
     6841 ttcgcccata cgattcttca caatcgtttc gatgccatca aatgcaccac ctacgatgaa
     6901 caatatgttc gtcgtatcaa tctgaataaa ttcttgatgt ggatgtttac gtccgccctg
     6961 aggtggcaca cttgcaacag ttccttctag aattttcagc aaggcttgtt ggacaccttc
     7021 gccggaaacg tcacgagtaa tagatacatt ttcgctttta cgcgcaattt tatcgatctc
     7081 atcaatatag atgatccctt tttctgcaag ttccacattg taatcagcag attgaagcaa
     7141 tttaagcaaa atgttttcta catcttcccc cacataacca gcttctgtca aacttgtcgc
     7201 atctgcgatt gcaaaaggta cattcaatgt ttttgctaat gtttgtgcta aaaatgtttt
     7261 accagaccct gtaggtccga tcaagcagat attgcttttt tgcagttcaa catcatctgt
     7321 gttttcttgt gcttgctgat tgacacgttt atagtgattg tatacggcaa cagctaacga
     7381 gcgttttgcg cgatcttgtc caatcacgta gttattcaat acttcaagaa tttctagtgg
     7441 tttagggact tcagtgaatt cgcgtacagc ttcttcataa aattcttcat caatgatttc
     7501 tttacatagg tcgatacatt cattacagat gtagacccct ggaccagcta cgatcttttt
     7561 cacttcttct tgggttttcc cgcaaaaaga acagcgtacg gtctcattgc tacttggatt
     7621 gtcatacatg gtcttcaccc cttaacttgc aaaaagtatg caagaaacta tcaaagactt
     7681 tacagtcttt tactatcata acatagttta gtaaaaagaa aaagtgttta acaaaggatc
     7741 aactgaccta tacctttgca ttttttactc ttaacaaaaa ataaggaact gtgacaaaat
     7801 cgtgtcacag tcccttattt cgaataaaca attttctaga agtagcttct tcagaaatga
     7861 tttattactt gatttctatt ctttcgcttc tgtcgctgta cctgtgatca attcgattgc
     7921 ttttttcatt gtcacatcat gttccagcat atcttctgtc aacacgcgtt tgatttgttc
     7981 gattggcatg ttgtatgttt ctgataattc ttggatttct gcattgattt cttcttctgt
     8041 tgcttgaaga ttttcagcag ctgcaattgc ttcgatcaca agatttgttc ttgtgcgcat
     8101 ttctgcttca ccttcgaatt gtttatgcaa gtcttcttct gtagaaccag ttaattgata
     8161 gtacatttca ggagagatac cttgacgttg catgttgttc aagaattcgt ccattgaacg
     8221 atgtacttca tcatgaacca tcacgtgtgg caattcaacg atttctgcgt tttcaacagc
     8281 catacggatc gctgcttcat cttttgcatc gtccgctgct ttttcttttg tttctactaa
     8341 ttctttgcga tatttttctt tcaattcgtc taatgttgat acttcgtcat caacatcttt
     8401 tgcgaattcg tcatctaatt caggcacttc ttttgctttt acttcatgga ttgtcacttt
     8461 gaagactgct tcttttccag caagatcttc tgcttgataa tcttctggga aagttacatt
     8521 tacatctaga ctttcacctg atttatgacc aactaattga tcttcaaaac ctggaatgaa
     8581 tgagcctgaa cctaattcaa gtgagtaatt ttcgcctttt ccgccatcaa acgcttcgcc
     8641 atctacaaag ccttcgaagt cgattactac tgtatcgcca ttttcagctg cagcatcttc
     8701 tttgataact aattcagctt gtgcttcttg ttcacgtttg atacgtgcat ccacgtcagc
     8761 atctgttact tcacggtctt gtttttcaac aactagatct ttatattgtc ctaattttac
     8821 ttctggtttc actgttactt cagcagtgat tacccagtct tgaccttttt ccatgctttc
     8881 aacatcgatt tttggttgag aaacaggatc gatgccagct tctttgacag cagcttcata
     8941 agcttctggt aatacagcat tcaatgcatc ttcatataaa gcttcttcac cgtacatacg
     9001 gttgaatact tgacgagaaa ctttcccttt acggaatcca ggtacgttca agcttttttt
     9061 cactttatta aacgcagttg ttaatccttt ttggatttcg tcttgcgcaa tcgtaaattt
     9121 taaaacacca tcattcgtgc cttttttttc aaatgttgca gtcatttaat tccctccgag
     9181 tttagaaatt cattttatac atcaaaaacg tttcatgcat acctttaaat agtacactaa
     9241 ccctttcaaa ttgtaaacat attgaatgga tagaatcctt gtttttcata acatttttat
     9301 ctttttcact gataaaatct aatggaagaa gatcatttgc tgcaaaattt tttctctttt
     9361 ttttaaaaaa tcttgttttt catcttctgt accttcatac atcgtttttc ctgtacacgc
     9421 ctctacagtc agttgaaacc agcgcttcgg gtcttcgatg tatgtatcat agacaggata
     9481 aacaattgca gattgcaacg tgaattcttc ttttaacacg gaactaagca gcgcatcttc
     9541 attcccgtaa cgatctgaaa atacacataa ttgctggtaa cttgcctgct gctgcggctt
     9601 tggcagatct tttggtatga ctttgactaa cttttcttca atcgtcaaat agaaaacagg
     9661 atggcattcg ttcaattgcc taagtgtctc cagtaatttt gcccttgcta aaatatggac
     9721 ctctggcgat acaataagtt tactcgctgt ttggataaac tcatgataag gaagctgttc
     9781 gatctcatca atagacgcaa gctgacgtgc aggttccaac gaaggaagtt cctccgattt
     9841 attgtggagt gccttgatct tctgcctctc catttgtccg gataattctt ctgcaaattg
     9901 gattttttca atgacccgct gctcgaaagc ttcataaaaa tctgtacttg ctatcaattt
     9961 tctagcatat aaaaaatctt gtgtactgat taacaagtca aaataaaatt cagcaaactc
    10021 ctcattttga agatactgtt tttcatgcaa gagtgcttgc ttcaatgctt cttttttctc
    10081 gtccaactcg taaagacaaa aaacaagcag gctattagcc tcaaagcttt ctttcaactc
    10141 gtaagctcgt tgaaataatt cttttgcctg ctgatattgc tggtttgcta gtgcttgttg
    10201 tgccactcgc attaaattct catatttttc tggaaaatca atcggcttca cgcagtgtcc
    10261 tcctcttgct catattcagc tcatgttcaa tagtagcaaa acaaaaaaag catgacaatt
    10321 aaaaataact gaagcaccct ctaaaagcta gattttagat ctgactttta gaaggtgctt
    10381 tcaactgtta ctgcttttta ttgctcatca gctgttgact tcctggttga acgctttgat
    10441 cagattgatc gtaacaacac ttgtattagt gaaaaaatcg cccagatgtt gctttttctg
    10501 attaaagact gctggaagat accctaacat cagcaaagga aattttagaa tgaaccggca
    10561 agcaccttct ctaactaaaa cggttgacca gctaagttct tcttcatcaa agctgatgac
    10621 tcgaatacca aagatcattt tcccaatcgt ttggccatga ttgaatttcg tcaataaaat
    10681 aaaatacgct agataaataa gtaaggctgt gaatccgtag agactcagat aagaatctga
    10741 aaaattaagc caacctagat ttttggcaat accaagtgtt aaacgcgtaa tagctccgat
    10801 acacaaaaga tcgaccaaga aggcaaacat tcggataaaa aaacctgcat agaaatactt
    10861 aggaaaatca tttgcttttg gcggttcatt tcttgtgtcc gtatactttt tccatttacg
    10921 ttgtttcttt ttgatttctt cgggcgtcaa aggctctctt tcaaaagagc tcagtacctt
    10981 ttgggaaaag ttggagtcag gtcctacagc aggctgctgt tgcttatctt tttcttgttc
    11041 cttattttca tttgaaagct ctgacatgct tactcacctc cgtaataata cattgctttt
    11101 ggtgattccg ctgtccctag attctcaaga acggataaga tctgactagt ctcagaaggc
    11161 ttcaatccct ggaattctgc tagtttactt cctaaccatg tactagcaaa cccagatggc
    11221 gtggaagaat attctactag ttccgcatct tcaagctgtt gttcctttcg tatagctttt
    11281 aaagcatctt caggaaatcc caattcatcg actaaaccta cttcttttgc ttggacacca
    11341 tcataaatcc ttccatcagc aatttttttg acttcttctt cagatttatt ccgtccctcg
    11401 ctgacgattg ctacaaaacg actatatgcg ctgtcgatat atgcttgaag caccgcttct
    11461 tcttcttttg tctctggtcg cgttcctgaa cccatatctt tcaacgctcc gcttttaact
    11521 gttgtatctt ccacacccag tttttctaga agtcctgaat aattcaatcc tgacatgatc
    11581 acaccaattg agccggtcac tgtttcttct gttgcaaaga ttttatctgc ttgagcagaa
    11641 atatagtagc caccgctagc tgccatgttt ttcatgctga catacattgg gatatcatgt
    11701 tctttacgaa tcgtatccaa tttcttagcg atttccgcac tttcgtagac accgccacca
    11761 ggagaattta cttctaaaaa gatacctttg actgttggat cttcttcaat cgccttcagt
    11821 tgttccataa aatttttatg attgtaacct tcagaagcaa ataaacctga agaccctccc
    11881 gcagcaatcg ttccatctac tgataatttt acgatttttt gcgtactgtc tccctcttcc
    11941 aacacatttg gagctagttc ttcatcccca tataaccaac tgttcaatcc ctcaatttga
    12001 tcgttttcct gaggcttagt caaactagct gaaactaacg aaaccacaaa taatcctgca
    12061 gcaatcagaa ctgctgccca acgtctttta ttcattcttt ctcctttcta atacaagttt
    12121 tttatgacga tcacagtttc tgactgctca gaagtgtcga accgctctgc tttttcttcc
    12181 cgcgcatacc cggaaaacga ctcttttgtt aattcatctt ctaccgtaaa ctcacgagct
    12241 tgagcaactt ccaaggcatc aggtgcgatt tcttggatgc tatcccagcc agtatctaaa
    12301 aatattttcg tatcatctga tacaccagct gatgcttcga atttggacaa ttgttttttt
    12361 aacattccca gtgtcatgat ctcaattttt gaatccattt ttactcctcc tctatccgat
    12421 tactttctat tgtaccgaac tcctgaaaaa gatgatagat aaaaacccat tattttttga
    12481 cataaaaagg acatttgaca aagaatttga tatataccaa tttatttttt attctttttt
    12541 tgttgacaag aacattcgca aaaggttatg attttaaatg tttccagata aaaaacaaaa
    12601 aggaggatag ttataccttt taggatagcg ccaggtctgt gtaaacagat gacgaggaga
    12661 gggtttatcg aaagattcgg cggatggccc tagggactgc actctacaga aaatccacaa
    12721 aaaatgtctg caaagataaa acaaaaggat tttcctgcag ctggaagcat tcatacgaat
    12781 tcattataga aagtaggatg ttttttatgt gcggaattgt tggaatgatt ggattaaaaa
    12841 atgtaacacc aggattgatt gacggtctag aaaaattaga ataccgtggt tatgattcag
    12901 ctggtatctt tgtctcagat ggcacaactg attatctggt caaagcacaa ggcagaattc
    12961 aaaatttgaa aaacaaaata aatattgata ctacaggaaa tataggtatt ggtcatacac
    13021 gatgggcaac tcacgggcaa ccttctgaag aaaacgccca tccacatact tcccaaagtg
    13081 gacgcttcgt gctcgttcac aacggcgtaa tcgaaaactt tgaagaactt aaaattgctt
    13141 atttagcaaa tgaccacttt attggtgaga ccgatactga gattatcgct catctaattg
    13201 aaactttcgc gaaggatact acaacgcaag aggcgttttt aaaagctttg cgggtcatca
    13261 aaggttctta cgctttcgct ttgattgatc ggacagcacc tgatgttatt tacgtcgcta
    13321 aaaataaaag tccactatta atcggactcg gcgatggatt caatgtgatt gccagcgatg
    13381 caatggctat gcttgctcat acgaaggaat tcgttgagat tgaagatgaa gagatggtta
    13441 ccgtcacatc agaaaaagtt attattcaaa attttgcagg tgacatcgta gaacgttctt
    13501 cattcgaagc acaagtagat gcttcagata tcgagaaagg tacctatcct ttttatatgc
    13561 tgaaagaaat cgacgaacag cctattatca tgcgtcgtat cgctcaaaaa tacgtaacag
    13621 aagacaatcg tgtctctctt gatcaaaagc tggtcgatac attgtctgat agcgatcgaa
    13681 tctatatcgt tgcttgcgga actagttacc atgcggggtt agcaggtaaa cagactttgg
    13741 aaaaattgac acagattcct gttgaggttc atttagccag tgaatttggc tataacacac
    13801 ctcttctttc aaaaaaacca ttctttattt tcttgagtca aagtggcgaa acagcagaca
    13861 gccgccaagt gctggtaaaa atcaaccgtc tcggctaccc ttcacttacg attacaaatg
    13921 ttgctggttc tactctttcg cgggaagcat cctttacact gctattgcat gctggacccg
    13981 aaatcgcagt agcatcaacg aaagcttata ctgcacaaat agctgtacta actttattag
    14041 ccaaagctat cggtgataaa aaagaatttg aaacttctct tgtattcgat atcactcatg
    14101 aattgagttt agttgctaat gcgatggaat cggtcatcgc ccaaaaagat tatttagaag
    14161 agttggcaga agtatatctc agcgatacaa gaaatgcctt ttacattgga cgtggtgctg
    14221 actacaacgt ttctcttgaa gctgctttga aattgaaaga aatcagttat atccaggcag
    14281 aaggttttgc cgctggcgaa ttgaaacatg gaacgattgc tttgatcgaa gaaggaacac
    14341 cagtgatcgg aatcatcagt gaagaagtga ctggtgcgca tactcgagga aacctgaaag
    14401 aagtcgaaag ccgtggcgct aaaacactag ttattgtctc agagggatta gaaaaggaaa
    14461 gtgaccagct ggtgctacca actgtccacc cttatctaac tactctaacc acagtcgtac
    14521 cgactcaatt acttgcatat tacgcaacgc tattacgggg attcgacgtt gacaaaccac
    14581 gtaatttagc gaaatcagtg acagtagaat aataaaagaa caaggaccag tgtcagacat
    14641 ctgacactgg tccttgttct tttatttttt atgaaaatat ctcttaatcc agtccgattt
    14701 cttctttgac tactgccgcg attttatcga catagtaatt aactttctca tcagtaggcg
    14761 cttctgccat cactcgaagt aatggttctg taccggaagg acgaactagg attcttcctt
    14821 ctgatcccat ttcagcttct gcctcttcaa tcactttttt gattgccgga acatccatcg
    14881 caccgttttt atcacttacg cggatattca ctaatttttg cggatagatt gttacctctg
    14941 ctgcaagctc tgataatttc ttacctgtct gtttcatgat attcagtaac tgaattcctg
    15001 aaagcatccc atctccagtt gtgttaaaat ctaagaaaat catgtgccca gactgctcgc
    15061 cgccaaagtt ataatcgttt ttgcgcattt cttctacaac gtaacgatca ccaacttgtg
    15121 taacgacatc tttcaaaccg attccttcca ccgctttgtg gaatcctaag ttactcatga
    15181 cagtcgtaac gatcgtatct tttttcaatc gatttttttc tgccaagtat ttagcacaga
    15241 taaacatgat tttatcaccg tcgacaatgt ttccaagttc atccaccgca ataatacgat
    15301 cgccatcgcc atcgaaagct agtccagcat ctgctccttt ttcaacgacc atctcagcta
    15361 aacgttctgg atgggtagag cctacgccgt cattgatatt taacccgtct ggatttgtgc
    15421 ccattgtata aaaatctgtt tcaagatcgg caaacaaacg attaacactt gttgaagttg
    15481 ctccgtttgc tgcatcgaca caaatggtca aaccggaaag gtcaccgttg atcgtttgta
    15541 caaggaattg agaatatttc aataagcctt ctggaaattc ttctacagtt cccaatcctt
    15601 cagctgatgg acgaggcaat tcatccactt cagcatctaa taatgcttca atttctagct
    15661 cttggtcatc tactagttta aaaccatctg caccgaaaaa tttgattcca ttatcttgtg
    15721 ctggattatg agaagcagaa atcatgaccc ctgcgcttgc tttttgcaaa cgtgtcagat
    15781 aagcaacacc tggtgtagag atcacaccta gctggaacac ttcgataccc accgataaaa
    15841 gtcctgaaat cagcgcttct tctaacattt gacctgagat tcttgtatcc cgccctacta
    15901 acacgcgtgg gcgttttcct tcttcttcat gctggcttag gacataacct ccgcaccgtc
    15961 ccagcttaaa tgcaagttct ggcgtaagtt ccttgttggc ttcaccgcgt acgccgtctg
    16021 ttccaaaata tttacccata gttgtaaaat tactccttta acttaatttt ctgaggaatt
    16081 tgttctgctc atttgtgttg attcgcttgt ttctgatgtt tcttctgtac ttgtggtcga
    16141 gctgcttgtc ccagattccg aactggaaga aatggttgtt gtcgatggtt ccgttcctga
    16201 atcagcagat gaaatacttc tactcgtact tgtattttct tgtgtccgac tgctcgtcga
    16261 agcagagcta ctgcttgacg tttgcaacac gggtgtaatc tccacactaa ctgattcggg
    16321 cttgatactt aacccctctt ctactggtat ttcaatttta cgagttgttc gatgattgat
    16381 acctgagaca ttgatcggta cagcaatact gtcaccgatc gatgcaagca aatcgctaga
    16441 gccgcttact tctgcttcca gttttgaaaa actgaaatca tagctttcta ccccggcagc
    16501 tggtgttcct tgctgtatcc cataaagtcg taccgacttg cttggcttga tcgaatcagc
    16561 tctcactgtt acttgcactg gatcagagac gatacttaat acttcccctg ctgcattcag
    16621 cgcctgtatt gggacctcac tattaatccc atcatctgtg accatcgaag cgtccacatc
    16681 agctactact cgtttgattt ctgccaatgt tttgtcacct gtagtgatag agactttttt
    16741 aggactaacg gaagtatcat caagttcata tccagatggt gtgaacgttg aaggaatcaa
    16801 cgtttccaca tcgaaatctt ttattacctt tttctcaacc gttactgtaa tagtcgatgg
    16861 ttcgatcttt gctgtgacag cactgctcaa attttgaatt tttagcggaa cttcatgtgt
    16921 tccttcgccc agcttagtga tatctgctgt gacgcggaac atccgtgtat cttcgtttgc
    16981 ctctgcattc aactgaactc ggtttgcact ggaaagtttg acagtcactg cattttcata
    17041 tccatggata tagtagcgct ttgaatcata agaaatcttg atcggcaccc ccgtaactgt
    17101 ttgattatac gcttcatttc cagaaagcgt atttggaacg gactggccat ttgcattgaa
    17161 gtacagcgca atactgaaca gcaatgcaat aaaactgtaa agaatgcttg tccgtttctc
    17221 ttttgagatc atttgcgatc ccctccctta gtcataccgt ctaggaaaga ttgaatcacg
    17281 tttttcttgt tttttgtttt ctcttcagca attagttctc tacgcaagat tgccaaatat
    17341 tcttgctggg ataagccagc catcagttca ttatctaacg tgatgctgac gtccccggtt
    17401 tcttcagaga caacgatggt aatcgcgtca ctgacttcgc ttataccgac agctgcccgg
    17461 tgtcgagtcc cgaattcttt agggatcaac atactttctg atagaggaag ataggcgctt
    17521 gctactgcga tctttccttc ttttacaata actgcaccgt catgcagcgg tgtattagga
    17581 ataaagatat tgatcagcag ctccccagtg atatctgcat ctaaagcaat ccctgtctca
    17641 atatactcat ctaatccagt atgacgttcg atagtgatca acgcaccgat ttttcgcttg
    17701 gacatatatt ggatggcttt gtcaaaagac aagatcatct tctcatcttc ctgctgttcg
    17761 ctttttgatt gtttgaaaag cgaactgcgt ccaagatgtt ccagtcctct ccggatttcc
    17821 ggctgaaaaa tcacgatagc agctatcacc ccgtacgtaa tgacttggtt catcagccaa
    17881 gacaaggtgt gcagaccaat gatttccgct acgattcgga taacaataaa aatagctaca
    17941 cctttcagaa gctgaatcgc ttttgtgcct cgcagcagtt ggatcagttt gtatacaaga
    18001 taccagacaa ccagaatatc aagtatgttc accaaaaaat tcagagagaa caagtcaccg
    18061 gaaaacattt gctgccaata gtccaaatta aaaagttccc cgatttgaaa tgacataaaa
    18121 agcctctctt ctcttttgag atttcctctc tagtatatca tatgttttaa ttttagaggc
    18181 tgatatgctc gtatttttct tgagaaaaac gcataatttt caagaaaatt gcaagtatgc
    18241 gttttatttt tctctgcaat aaatattttt aaacttcaga gaattattca ttatattttt
    18301 tctgttattt ttcctttttt tcttttgtag cttgttccat ttttttacgt cgttctgctc
    18361 tggcagctct tctagcagct cttctttctt ctgcttcaag gtccgtcgtt ttttcttttc
    18421 tagtcttaac gggtacatcg atagattcct ctggttcaag tcgtttcttg attttttcta
    18481 attctcccgt cagtgcagca tagttgtaaa aacgtgtttt agcgtcgtgg acagtctttt
    18541 caaataaagc ttttgcaaga tttggatttg cgttatctag cgaggaaaag cgaacttgtt
    18601 tcctcatgaa atcaggcata gcagagaaat caggtttttt gtagtccaaa gtcattgggt
    18661 tcttgttttt ttcgaccatt gtagggttat atcgatataa tgaccaataa ccagaagaaa
    18721 cagcttcttt tgcttcttta agcgattctt tcattccgcc aagcaagcca tgtgtgatac
    18781 aaggcgtata ggcaataatc agagaaggac caggatactt ttctgcttct tcaaatgcct
    18841 tgatcgtttg catttgattg gcacctgaag caatttgcgc cacataaaca tttccatatg
    18901 tcatcgccat cattcctaga tctttctttg aagcatactt tccactagcc gaaaatttag
    18961 cgattgccga agctggtgtt gcttttgagg tttgaccgcc agtattggaa tagacttcgt
    19021 tatccaaaac aaggatattc acgtctgctc cgctggcaag tacatgatcg atcccgccaa
    19081 aaccaatgtc gtaagcccat ccgtctccac caatcatcca ttggctgttt tttacgaata
    19141 gatcctttct cttgtatata tcctccagtt cagggaaaat tgatcgttcg ctttcgatcg
    19201 cagcttgcag ttttgctgct ctttgctgcg ttccttcact ctcaaacatg tgtactaacc
    19261 agtcttccat caattctcgt agttcttctg atacttttgg caatacttct ttgattgaac
    19321 ttgccaaagc ctcgcgacga gtctgtgccg ctagaagcat accataacca aattcagcat
    19381 tatcttccaa taaagaattt gaccatgcag gcccttgacc acattcattg gtcgtataag
    19441 gagaagcagg agaagcaccg ccccaaatag aagaacagcc tgtcgcattc gcgatcatca
    19501 ttcgatcacc gaacatttgt gtcaataatt ttacataagg tgtttcgcca cagcctgagc
    19561 aagcgcccga gaattcaaga agcggttgat tgaattgtgt agacaagaca gtattctttc
    19621 ctttgactgg atttgctttt tgtcttaatg tcattgcaaa agcccaattc acagcttgtt
    19681 ctttctgttc ttcataaggc ttcatcacta aagctttttc ttttgcggga caagcctcta
    19741 cacacagacc acaacctgta cagtcttcca ctgaaacttg gatacggtat tttacgccgt
    19801 ctgcgcctcg catttctctc acgataaatc cttctggcgc ttcttccatt tcctcttcat
    19861 ctgctaaaaa tggacggatg gcagcatgag gacaaacaaa ggcacattca ttacacatcg
    19921 tacagcgatc actaatccac tcaggtactt ctaaagcgac gccccgcttt tccatagaag
    19981 ccgttcctaa aggcatttcg ccagttgtca ttccatttgc tacaagtgta ccaacagaga
    20041 gttgatttcc ttcctgtgca ttcactggtt caagtatttc ttgtatataa ccagtgttcc
    20101 ttttattgat tttttcaggc atctccaacg ttctccaatg gtctgggatc gcgacttgat
    20161 gaagcagttc ggtcgtccga tcgattgctt gtatgttttt ttctacgata gccattgatt
    20221 tatgtccata gcttttcagg gcttcttctt ttagcagcgg aagcacctca tcaaaaggaa
    20281 tgatgtcagc taatttaaaa aaagccgttt ccattgccgt attgatccgg cgtcccaaac
    20341 cgacttcttg ggccaatctc atggcattga tcgtatagaa tctaatgtta ttttcagcca
    20401 agtaacgttt taattttaag ggcagatgcg tttctaattg ttcgtcgctc caaagagtat
    20461 tcaataaaaa aatgccacct ggcttcaatc ctttgaccaa gtcatagcta tgcaggtaag
    20521 ctggtgtgtg acaggcaaca aggtccgcat gctcgacaag atacgctgaa cgtatcggcg
    20581 tatctccaaa acgaagatga gagacagtca gaccgccaga ttttttcgaa tcataataaa
    20641 aatagccttg cgcatattta tcggtgtgat ctccaataat cttgatagct gatttatttg
    20701 ctccaactgt tccgtcagat ccaaatcccc aaaacttggc ctggaatgtt tgcggttctg
    20761 tcaaatccag actttccttt ggttccaaag attgataagt cacatcatcc acgatcccta
    20821 tggtaaaccg ttttttgcgt atgcttgggt cttttttcaa ttcatcaaaa actgctgaga
    20881 tttggtcggg agtcacatct ttagacccca atccgtagcg accaccaata acagccgggc
    20941 gaagttcaga atcatataac gcactttgaa cgtctaataa gagaggttct ccacctgccc
    21001 ctggttcttt acttcgatca agaacagcaa ttgtttttac tgtatgaggc aacttttgca
    21061 cgaatgtttc cactggaaaa ggacgataaa gatggatatt caaaaaacct gttttccgtc
    21121 cgtttgcatt caagtaatca atcgtttgtt caatcgtctg tgcaactgat cccatagaaa
    21181 caatgacttc ttccgcatcg tctgctccgt aataagtcac caagtcataa gaagttcctc
    21241 gtaatcggtt gatctctttc atatattttt gaacgatttc aggtaattta tcataatagg
    21301 ggttgatcgt ttcccgttgt tgaaaatgga tgtctggatt ctggttcgta ccactaacag
    21361 tcggatgatt gggattcatc cctcgtttac gaaatgaagc taatttctcc tgattgatca
    21421 gaggagctaa ttcttcatat tccaaaactt cgattttttg aatctcgtga ctcgtacgga
    21481 atccatcaaa gaaattcaaa aaagggactt ctccttcaat agaagcaaga tgggcaacag
    21541 gtgctaaatc catcacttct tgtacactgc tttcagctaa catagcaaat cctgtttgtc
    21601 ttgcagccat cacgtctccg tgatctccga aaatattcaa cgcatttgtc gtcactgccc
    21661 gactagcaac gtggaaaaca cttggcagta gttccccagc aatcttgtac atattcggta
    21721 tcatcaaaag cagcccttgt gaagcggtat atgtagttgt cagtgcacca gttttcaatg
    21781 atccatggac cacaccagct gctcctgctt cggattgcat ttctactact tttactggtt
    21841 gaccgaatac gttttctttt cctgctgctg accattgatc tactaattca gccatggtcg
    21901 aactgggtgt tattggatat attgcagcaa tctcggtaaa tgcataagaa atatatgcgg
    21961 ctgccgtatt gccatccatt gttttcattt ttcgcatgat tcatcatcct tgttcatatt
    22021 ctctcttatt atctgttcca ttataacgta aaacatgcca tttttttctt ttatttataa
    22081 aaatatttta catgtatcta tttgaattgg tcatctaatt tttagacaaa cagtctaaat
    22141 cgttgccttt ttttgtcaga catgtataaa ctataagcga aagaggtgtt ttaacatgga
    22201 tatatcagtg attgatgcaa cgaaaacaaa tacacaaaaa ggaatccttt atggttcatc
    22261 gaatgctcct aaaaaaatgg tcgaattcat caacttagct tgtccgtatt gccgacaatg
    22321 gtttgaagaa tcttatgagc tattagaaga agcagtccaa agcgggcaat tgcagcgagt
    22381 gatcaaattg ttcgataaag aaaaagaaag cttacagcga gggaacgtga tgcaccggtg
    22441 tctcacaatc agcgatggcc aaaaagcgat caaagagatc aaacaaatat tcgatactca
    22501 agacgagtgg aaacatcttt ctctccaaga agtagcggat tttgcagtag ataaactaaa
    22561 gctgacagaa caaaaagacg aacagctgag ccaagctgtc atcaacgaag ctgagcaagc
    22621 acatattcga tttgttccaa ctgtcatctt gggtaaagaa atctttgatg aatctatttc
    22681 aatagaggaa ttaaaagaat tgatccaaac aaaataaaaa aataaggagc gtg
//
LOCUS       DAZKNH010000039        22716 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000039 DAZKNH010000000
VERSION     DAZKNH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.039"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..229)
                     /locus_tag="ACG8MG_001402"
     CDS             complement(<1..229)
                     /locus_tag="ACG8MG_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFQ0375453.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEK"
     gene            complement(320..772)
                     /locus_tag="ACG8MG_001403"
     CDS             complement(320..772)
                     /locus_tag="ACG8MG_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0375454.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(785..1195)
                     /locus_tag="ACG8MG_001404"
     CDS             complement(785..1195)
                     /locus_tag="ACG8MG_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0375455.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1470..1682
                     /locus_tag="ACG8MG_001405"
     CDS             1470..1682
                     /locus_tag="ACG8MG_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFQ0375456.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1685..1897
                     /locus_tag="ACG8MG_001406"
     CDS             1685..1897
                     /locus_tag="ACG8MG_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375457.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            2037..2192
                     /locus_tag="ACG8MG_001407"
     CDS             2037..2192
                     /locus_tag="ACG8MG_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375458.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2189..2413)
                     /locus_tag="ACG8MG_001408"
     CDS             complement(2189..2413)
                     /locus_tag="ACG8MG_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0375459.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2491..2679
                     /locus_tag="ACG8MG_001409"
     CDS             2491..2679
                     /locus_tag="ACG8MG_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375460.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2818..2946
                     /locus_tag="ACG8MG_001410"
     CDS             2818..2946
                     /locus_tag="ACG8MG_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375461.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            3005..3340
                     /locus_tag="ACG8MG_001411"
     CDS             3005..3340
                     /locus_tag="ACG8MG_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375462.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3573..4514
                     /locus_tag="ACG8MG_001412"
     CDS             3573..4514
                     /locus_tag="ACG8MG_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFQ0375463.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4516..5406
                     /locus_tag="ACG8MG_001413"
     CDS             4516..5406
                     /locus_tag="ACG8MG_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFQ0375464.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5449..6249
                     /locus_tag="ACG8MG_001414"
     CDS             5449..6249
                     /locus_tag="ACG8MG_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFQ0375465.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6264..7115
                     /locus_tag="ACG8MG_001415"
     CDS             6264..7115
                     /locus_tag="ACG8MG_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0375466.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7316..7777
                     /locus_tag="ACG8MG_001416"
     CDS             7316..7777
                     /locus_tag="ACG8MG_001416"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0375467.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7803..8255
                     /locus_tag="ACG8MG_001417"
     CDS             7803..8255
                     /locus_tag="ACG8MG_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375468.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8361..8555
                     /locus_tag="ACG8MG_001418"
     CDS             8361..8555
                     /locus_tag="ACG8MG_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375469.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8586..8879
                     /locus_tag="ACG8MG_001419"
     CDS             8586..8879
                     /locus_tag="ACG8MG_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375470.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8810..9349
                     /locus_tag="ACG8MG_001420"
     CDS             8810..9349
                     /locus_tag="ACG8MG_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0375471.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            9424..9891
                     /locus_tag="ACG8MG_001421"
     CDS             9424..9891
                     /locus_tag="ACG8MG_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFQ0375472.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10638..10868
                     /locus_tag="ACG8MG_001422"
     CDS             10638..10868
                     /locus_tag="ACG8MG_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375473.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            11027..11860
                     /gene="terS"
                     /locus_tag="ACG8MG_001423"
     CDS             11027..11860
                     /gene="terS"
                     /locus_tag="ACG8MG_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFQ0375474.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11853..13268
                     /locus_tag="ACG8MG_001424"
     CDS             11853..13268
                     /locus_tag="ACG8MG_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0375475.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13280..14809
                     /locus_tag="ACG8MG_001425"
     CDS             13280..14809
                     /locus_tag="ACG8MG_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0375476.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14727..15779
                     /locus_tag="ACG8MG_001426"
     CDS             14727..15779
                     /locus_tag="ACG8MG_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFQ0375477.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15781..16098
                     /locus_tag="ACG8MG_001427"
     CDS             15781..16098
                     /locus_tag="ACG8MG_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375478.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16225..16848
                     /locus_tag="ACG8MG_001428"
     CDS             16225..16848
                     /locus_tag="ACG8MG_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFQ0375479.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16863..17753
                     /locus_tag="ACG8MG_001429"
     CDS             16863..17753
                     /locus_tag="ACG8MG_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFQ0375480.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17776..17991
                     /locus_tag="ACG8MG_001430"
     CDS             17776..17991
                     /locus_tag="ACG8MG_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375481.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18003..18344
                     /locus_tag="ACG8MG_001431"
     CDS             18003..18344
                     /locus_tag="ACG8MG_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375482.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18344..18715
                     /locus_tag="ACG8MG_001432"
     CDS             18344..18715
                     /locus_tag="ACG8MG_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375483.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18708..19103
                     /locus_tag="ACG8MG_001433"
     CDS             18708..19103
                     /locus_tag="ACG8MG_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFQ0375484.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19105..19482
                     /locus_tag="ACG8MG_001434"
     CDS             19105..19482
                     /locus_tag="ACG8MG_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFQ0375485.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19483..20091
                     /locus_tag="ACG8MG_001435"
     CDS             19483..20091
                     /locus_tag="ACG8MG_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0375486.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20091..20432
                     /locus_tag="ACG8MG_001436"
     CDS             20091..20432
                     /locus_tag="ACG8MG_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375487.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20674..>22716
                     /locus_tag="ACG8MG_001437"
     CDS             20674..>22716
                     /locus_tag="ACG8MG_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0375488.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWI"
BASE COUNT         8142 a         3326 c         4639 g         6609 t
ORIGIN      
        1 ctttctcttt ttttaataaa ataatatcat tttttaacat agtaatttgt ttatttaaat
       61 ctgatagtgt ttcttcctta cttaatataa atctttctct atcatgtaat ttatttagtt
      121 tttcatcatt tttttgttct aattcataag cttctttttt tattgtgtta gctttcgaca
      181 gtttagtgat tcctataatt aataaaataa gtccaatcaa taataacatt caaaactcct
      241 catttctttg atataattat tgtagagttt actcagaaat gaggaaagag tccatgtgaa
      301 agcatgggct tttttatttt taatgttgta atactaaatg ttcaagatta tgaggaagcc
      361 ctaaatattc aataatttgg aactgactca tatcagataa atcttgttca tctattgttc
      421 tagttaacat ttcaattgca aacatattag cttcagcttc tataccataa atcatacaac
      481 taaggttgtt tgctctaaaa gctgctgtgc aaataccttt atggagtcta acgtgggcta
      541 tttcatgcca taatacaaat cttttcatag aaggtgatag gttttgatta attaatatca
      601 cataacatct tttattgtgt atcgtttgtc ctaatgtttt attccctaaa tcagtttcaa
      661 ttaaatctat atcagaatat ttgattattt tatatggatt aatcgcatca gatccaaact
      721 ctacaaataa tttttctatt tcttctaata tccagtattt gtttaaatgc ataccaaagc
      781 ccacctattg acgatatttt ttaggagtga attttttctt tgctttaact ttggttaagg
      841 caagtgcaag ttgaatggca tcgtataatt tttccttatc ttcatcgtct aaaggttcac
      901 cataataatt tgtttcagca tctgaattca agccttcaag taatttttca gcttgtaaag
      961 caatatcttg tttatctttt tctgttaaat cataataatg gcgtttatca gtacgtccta
     1021 aaagataatc agttgataca ttaaagtaat ctgcaacttt ttgtatttta tctccagatg
     1081 gagtactttt attccatcta gacatgcttc catttccgaa atttaacttt ctttcaagtt
     1141 ctgccaacgt cattgaacgt tcttttaata agatttttat acggtctaat agcataggat
     1201 atgcattcct ttcaaaatac cgacaaaaaa agtatttttc tgcaaattat ctatttacaa
     1261 atagagaatt tgcagttata ctagttttgt aagcaagata tttacggcat acaaagacta
     1321 aaagcaataa actaatagtc tccccagaca tcagtaagct cgctttttgt gcgctgtatt
     1381 ttgtatgctt acataatagc atatttgcag tttttgtcaa acttattaga caaaaatatt
     1441 agaaaatatt cgataagggg gtatgaagca tgactttaaa acagcaagta ttaattgctt
     1501 tagtaaagaa aggttggagt caaagagagt tagctcgacg aatgggtata tcaattactt
     1561 atttacgaga tatcttcatc gaaaaaagaa aacctaagga acgtttaaaa caaatcgaag
     1621 aacttttgga tatcaagtta gaagtagatt ctaatgatca gccatcagaa caggaggcgt
     1681 aaaaatggta caacgtttga gcaaagcgga gtttaaagag aaatataagt attctgaaag
     1741 tacttatcaa agaagaatga aggaatttaa aaaatcacgt tttagtgagg ggtataacgc
     1801 agttacttcc aaagaaataa caattgatgt cgatttatat gaacagttta gaacatggcg
     1861 ttctaaaaat cgatttagag ttagagctgt ttgttaatag ctaaaaaatc taaattcacc
     1921 acttctgaaa agaggagttg ctgctctcct ttcaagtttg ccaaagtcca cgttttttca
     1981 ttttggcaac tcctcttctt ggaggtggtg acggcgagag acgaaggagg gatgacatga
     2041 aggaagaatc aaaaataggg attttgaatg agaacaatta cacaagatta attattgaaa
     2101 cagaagaagg gaaaaaagta gcagaagtta cattgaccga tgcaactcct gctgacggtt
     2161 atcgtattcg attaactcct aaatatgatt aaccttttgg tgggtgtgga tcgtttccgt
     2221 gactatcacg gctttgaatt tttccatctc tgccatgaat aaatagctca gatccttgat
     2281 ttttagaaat ctctctagcg atttttactg cttctgattt tgtattagta tgtgcagtcg
     2341 ctttagaatt tccagcacct ttgacattcc aaccaccatt ggaattaggt acaacatgtt
     2401 gattttttcc catagttttc acctccattt ctatagaaat attttatcaa atagaaatgg
     2461 agaaattcaa taaacgaaag gagaagtgat atggcatata ccttgcaaca agaacatcaa
     2521 attctcggtt tgattaaaca acgcaggaaa caattacaag atgaccgtgc agcgcttaga
     2581 aaagccgatg agctatcaga taggcaagct gaactaattg cttctgaact tgaggatttg
     2641 agaatgctag aaataaaaaa tagggagatt agattatgaa gaagacagac acacttttta
     2701 taggattcat tttggggtta ttagtgattg tagcgcacca aagtattatc gggggaagct
     2761 tgttcgcagc attgatggtt ttaatcaatc tgcttgattc aaaagaaagg agcaactatg
     2821 gcacgagaag aagcgctaaa aatcggtaaa gtgattgctg ataatcggtg gggaaatagc
     2881 cgccctatta ttttaagcaa gcaacatatc gaaaagcaaa aagcatggca acaaataaaa
     2941 aagtgactct gccagccagc aaagagtcac atacaaattt ataaaactag gagaattata
     3001 tcacatgaat aagaaaattg aaaatcttat cgaagaattg aaacgtgaat gccaaaagca
     3061 aggtgtttct attatttgta cagcccaaaa agaaggggaa cttaaaagtc tcgtttatgg
     3121 tgagacaaca gaaattttac tttgtcttgc tatgcaagaa gaacatttag atgaaaatct
     3181 cccattgtca gcacatatta tgcgtagaat tgctgtggat gcttacgaac aagcgaaaaa
     3241 tgaagaagaa aatcaacctt caaatcatac ttttgttatt aataacaagg aagatttagc
     3301 tgatgtgatg actcgtattc tcaaggggga atttcaatga tgaatgaaaa aacaattgat
     3361 ctactcatta ttttattttt cacgttagtg attgtagcga aaagttatgt aatctttggc
     3421 ttgttattcg caattaccat gcttgtgctt tgcgtgttag caagcagaaa agaggttaaa
     3481 catgaacgag ttagcaaatc ttgacaacta tttaactgat cctgattatg ctaaaccacc
     3541 atatgaagca ccaattgatg aggaggatga agatgagtaa atctacctta gaaatgagcc
     3601 atcaagaatg gcttgaagac cgtaagaaag gcatcggagg ttcggatgtt ggaacaattt
     3661 taggattgaa taaatggaaa tctccttatc aactatggct agaaaaaaca ggacaagtag
     3721 tacttgaaga atcaggaagt gagcccgctt attggggtaa tgttttagaa gaagtggttg
     3781 ctaaagagtt tcaggaacgt acaggcaaaa aagtacgcag aagaaaccaa gtatttgaac
     3841 atccattaca tccattttta agagcaaata ttgatcggga agtagtggga gaaaatgcca
     3901 ttctggaatg caaaacagcc aatcaatttc tcggtaaaga atgggaaggc gaagaggtac
     3961 cgcttagcta tctctgtcaa gttcaacatt acatgaacgt tctaaacaaa gactattgtt
     4021 acatcgctgt cttaatcggt ggtcaaaaat ttatctggaa gcggattgaa cgagatcaag
     4081 agctgatcga tacaatcact gaacaattag tagagttttg ggaaacaaac gttcttggag
     4141 gtatcgagcc aattattgat ggtagtcaag cgacagctga tttcttgaaa gagaagtatg
     4201 cagatgttga agacgttcaa acgacgttac caattcattt cgatgaacta gttgaacaga
     4261 aaaacgaact caaacggact aagaaagaaa ttgaatcagc tatccgtcaa gtagacaacg
     4321 agattatcag cgagctaggt aaacgtgaag ccagtatcgg tatcactcaa aaaaacatca
     4381 tcagttggaa atttgtccgt acgagacgta tgaactcgaa gaaactagca gagaaatatc
     4441 cagatgtcgc aaatgatgaa gagatttata acgttactga atcaagaagg ctaaccgaaa
     4501 gggagatcaa ataatatggc aacaaatgaa tcgttaaaaa atcaattggc agcaaagcca
     4561 cagaaacaag ttgcaccagg tcaattagga cttaaagctt tgatgaatac accaacaatg
     4621 agaaagaagt tcgaagaggt gcttcatgac aatgctaatg cttttatgtc gaatgttatg
     4681 actcttgtat ctaatgacag ttatcttgca gatagtgaac cgatgtctat catgagtggt
     4741 gcgttaactg ctgcaacatt aaatcttggg ctagataaga atttaggtta tgcatattta
     4801 gttccattca atagtaaaaa caagcaaaca ggaaaatggg aaaagaaagc tcaattcatg
     4861 cttggctata aaggatatat ccaattagcc caacgatcag gtaaatacaa agcattaaat
     4921 gtgatcgaag tttacgaagg agaactaaaa agctggaacc gactgacaga agagtttgag
     4981 tttgatccaa atggtagaac gtctgatgaa gtcattggat atgttggcta tttcgagtta
     5041 ctgaatggat tcaaaaaaac tgtctattgg accaaacaag aaatcgaagc tcatcggatt
     5101 gctaacaata aagatcgcga taagacaaag ttaagtggtg tgtgggcatc tgattacaat
     5161 gcaatggcac gaaaaactgt tttgagaaat cttctttcta aatggggaat cttgtccatc
     5221 gaaatgcaag aagccaccac atcggatgag agagtccaaa gggttcaaga agacggcagc
     5281 attattgctg aaacagaagt tgaagaagat attcctgaaa gaaaagaagc agaggttata
     5341 tctgaagaaa atgaagatgt acaaactgga ttatttgatg catctaatcc gccgttaaac
     5401 aaataatgag ggagctttct cccttgcttt tctagaggag aaatacgaat gaacacagga
     5461 tatgtcaaat tacatcgaaa agtgatgaat tcattcgttt ggaccaatcc ttatatgtat
     5521 aaattatgga acttgtgttt gatgaaagcg agtcatgaga atcgcaagat actttttaat
     5581 ggaaaagaga tacaagtgaa cagcggagaa ttcgtcacag ggcgcgatgc tatcacatct
     5641 gagatgaacg aaggcgtgaa acgtgaacat caagtgaaca gcggttctgt atggagatgg
     5701 ctaaaacaat ttgaaaaaaa cggaatgttg aacatcaaat caactacgaa atacagcgtt
     5761 atatcaataa aaaactggtc tttgtaccaa agcagtgaac aacaaatgaa catcaaacgc
     5821 acaacaagtg aacaacaagt gcacacaaac aagaatgaaa agaattataa gaatgaaaag
     5881 aataataaaa catcatcgtc gcaaccacgc aaaaagcgtg tttacgacac cgactcagtt
     5941 tactacattc tcgcggagga gttattcaaa cagatttgtc agaatcagga aatcaaaaag
     6001 ccgaatctgc aaagttgggc agatgacatt cggaaaatga tcgagatcga caagcggacc
     6061 gaaaaccagg tacggggaat gattgaatgg agtcagcaca atgtgttttg ggcatcgaat
     6121 attttatctg ctaaaaaatt gcgagaaaaa tacgacacaa tggcagcgca agcaaatcgt
     6181 gattataaaa caaaacaaac taaaacgctt gaatacgaga aatttagcac agatgagttg
     6241 cctatttgag aggaggcgta ggcatggaaa ctgttgggga aatcatggaa aagctgatac
     6301 agaaagtgct tgtccaacgt ggcgaatgtc ctgaatgcgg acagcctttg tatggatggc
     6361 gtacgaagaa ccctgatggc tcagaacgtt gtaagccaac ctgtatgcaa tgtgggtata
     6421 aagcattgcg agttcaggaa gacttacaaa cagaacgaat ttacaacgag agcctgaaag
     6481 caagagcaat caattttttc aaaggtggtt ctgttgtgcc taatcaagcg ttgtttgatt
     6541 gcacattgca gaattatcaa attgtcgatc aagaaacaag acaagcggtt gaagtaacca
     6601 aacgctttgt taattcagtc ttgttaggaa atccaagtca ccttgtttta actggaaaac
     6661 aaggaacagg taaaagtcac ctagcgatgg cagcggcttg ggaagtcttg aagcgatcaa
     6721 actacgataa aaaaatctta tttatcgggt tacaggaaat gctggatcaa atcaaatttt
     6781 cgtacaacaa tcctgaactc agaaaaacga ttgagggatc gttgattgca gatatcaaaa
     6841 cagcggattt agtcatcatt gatgatatcg gttcagaact aggaaaagat gcatcagata
     6901 gtcgagcgtt tggcataaac acgctaaatt cgttcttgga tgcacgacag aacctagcaa
     6961 cgattatcac aacgaacttg cttggtgaag aactgaaaaa agcttacggt acgagaacaa
     7021 tatcaagaat gtttgtcaac tctgatggat ttacgatggt attttctcaa acagcagaca
     7081 agcgcataaa accagtcaaa ggagcgatcg catgaatttg actaaacaag aaaaaagaat
     7141 ctgtcgtcag ataatgttgc aaattatagg tgatcgtgac atggacgaat tatctcaaca
     7201 agagagtcga atggttcgga agataagtaa gctaattatt tatcgggata cagaaagatt
     7261 tctcactgtt ttaggtaacg tctacgagaa tccagagtta ttggaggtag agtaattgcc
     7321 aaaaatatta gatgcatgtt gcggtagtag attgttctgg tttgataaga acaattctga
     7381 cgtaacattt atggattgtc gtcagcagta tgaagaatta tcaacaggtc atgtgatcaa
     7441 tgttgatcca gatgtggtcg cagactttcg cgatatgcct tttgaagatg atgtgttcga
     7501 tatggttgta tttgacccgc cacacttaag acatgtcggc gaaaactcat ggcttgccaa
     7561 aaaatatgga aaattagacg aactttggcc agaagacatc aggcaaggtt ttgcagagtg
     7621 tatgcgagtt ttacgacctt caggaacatt aatttttaag tggaacgagg aacagatacc
     7681 actttctgat gtgttagagg ctattggaga acagccattg tttggtaata aacgaagcaa
     7741 aacacattgg cttgtgttta tgaaaaaatt cagctaacgg aggaaacaaa ggaggaaaag
     7801 taatgaagat tgtattaaat aaatgttttg gtggctttgg gttgagccat gaagcaaaaa
     7861 tggaaatttt taaaagaaaa aatattgaag tattcccata catcaataac tttagctatg
     7921 actcagatga tgaatacacg agatacacag gccaaaagct aggttcgatg gattttatct
     7981 actatttcaa aaaagacccg aaaatcgaca aagttacagg aatttatagc gaaattgacc
     8041 gactgtacgg aattgctgat gattccagtt tttcaagtga ttcaaacaga ggtgataagg
     8101 acctcgttgc tgttgtcgaa aaactaggaa gtgaggccag tggaccatat gcaagtctta
     8161 aagtcgttga tatacccgat ggggctgagt gggaaataag tgattatgac ggcgttgaaa
     8221 ctgcacatta tggttttcaa acaggtagta tttagtccac taatgacaac taaagaggag
     8281 gcagaaggat gagtgaagat gaatactttg aacagtagtc tattaacgac agagattttt
     8341 aactggaaag gggtgtagtc atggacgcat cggaagtagt aaataaaatt aatagtctca
     8401 aagaggtttt cggagacgtc gaagtagtga taaacgcaca aggctatgac aacgaatttt
     8461 ttaagcagat taccgacatt agcattcagc aaggaatgga agacgaagac ggaaacttta
     8521 tcgatgaagt agccatttta gtgacttgcg agtaagtaat tgacagctaa agaggaggaa
     8581 gcggaatgaa actaaaagac ggattttact ccagcggcca cggcatcggc ggtttaatgc
     8641 tagatatgcc gacaaagaat cctaaaacac gtaagaaacc aaaattcaaa gtcggtgaca
     8701 tggttcgctg cgaagcagaa ggattcatct atccatttcg tggatatgta gaacacgtct
     8761 ataatcactc agcaatcatt cgcattgaaa acacgatgga atgtgataag tggttagcga
     8821 aaagcaaaga gaatttagca gtggtgagat tggtggacat ggaagttata aaatcttaat
     8881 tttgcttttt tacaactatc ctacaacaat gagcgttatt ccacaactgg aaggagaaga
     8941 ggcaattgtg aacattttag agaatataga tattaagcaa acaaggaaaa atgctagacg
     9001 attactaaaa agatacagaa atttagaacg tttggtcgga ccagtgaaaa tagacttttc
     9061 cgtgatgact gttaccaaaa atttgaaatt cacaattgac agtcaaaacg aagaaatcat
     9121 tgaagcgata agtactcgag attcggttat cgaagcatta acacggctaa gtagaatcca
     9181 tttccaggta ctttattata gttattgttt tcctaataag atgtcaatgt atcaaatagg
     9241 agaaaaatta ggatattccg atagaactat cgagagaatg aaggcagtag ctttggttga
     9301 atttgctgag gcgtataaat caggagaact catttcacgt acaaaataaa aaaagtcgga
     9361 ttcctccgac tatgaataat atttccgaca taagtattat atcataattg ggggaatcag
     9421 aggatggtac ttttcgatgt aaagaaatat gagacaccaa gcgcgaaaga tgttgatatg
     9481 gaacgcacaa aacataatgt cgctgtgttt ctttcagcat atctatcagc tagatgtaga
     9541 gtagggcaac atcgagaacc aaaagtgaca gcatcttatt ccttggttcc accttctaca
     9601 gctgatcata tatttgaagc agaaagaatg ctgatcgata aagaagaagc acaggaagaa
     9661 tttgagtatc tgcataaatt gtttgttcga ggctattcag cgatacagca tccgcataag
     9721 cctgatgtga ctgaaaggcg caagaagata ttctatgatc gttatatcaa tggtctgccc
     9781 atttatgtaa ctgctcaaag gaataatact agcgaagaat cggttaaagt agaatcaaac
     9841 agaattatca tccaatttgc ttcatcgtta gaactggttg ctttcaagta gccagttttt
     9901 acacttttta taccctttta ttaccaattt ggtttccatt ttataccttt tttataccaa
     9961 tcacttacct attcaacgtt gtattatgat agtgtcgaaa aattaggaaa caggatcgac
    10021 aaaataaaat gtaagggagg aaatctccct catcgtttaa ttaagcttcg ataggcagca
    10081 acggaaatat taagaataag gatgtgaatt tcaactcctt ctaaattgtt cttattatct
    10141 atcatccgtt gctgtctatt gtttttttaa ttattcacac gataactaaa ggtaagtgaa
    10201 gagaaatgat tccattaata atttcaattt ttgcgctttg tcttaatgta tatatgattg
    10261 gatttaaaaa tgggcaaaat aaaaaatagt agcagccaag aataatttta tagtgtcact
    10321 gtggcggaaa gggtagacgc taagcatgtg tgctaggtca atgcttcggc aaccatgcaa
    10381 ggttcgattc cttgccagtg actttggcaa ccgaggcatc ggcggtttaa aaatataggg
    10441 gtgcgcaatt tcgtacgcgt tttgtgcatc gtgtaggttg ctattacata actggttagg
    10501 ttagattgag atttgggatt cggtacaaat gaatcgtcaa atgtctcaag cacaggatcg
    10561 gaaacgtccc tgcctgtgca ttacatatta gatcactctt tgagtggtct ttttctttac
    10621 ataaaggagg ctacataatg agaaactact ggtatgtatc gctaactaat gaatatcctc
    10681 gaaccattga tgattgttca gtgcgtgttg tgcgttctgt acaaatcaaa gggaagtact
    10741 ctattgtcga aatggggaga gaagcagaac catgtgagat tgatgcgtgc aagctcgttt
    10801 attgcggtca tggtttctat gatgaaccaa acattcaaaa taatattaac aagaatttga
    10861 gggattagaa tgcaagaaat ggcttaaaaa tctccaatca ggacaaatca agttagacaa
    10921 agtttccgat ttaaagatat tgattgaagc tgatttaatg ctaaaagata ttgaaaatta
    10981 gaaaacaaaa ctcaacctaa acagaatgtg aggtggtgtg tattgaatgg caagacaacg
    11041 tgatccaaga cgtgatgaag ccaaaagaat ttggttagaa tccaacggag aaaagcagtt
    11101 aaaggaaatt gcatctgaat taaatgtttc agattctcag gttagaaaat ggaaatcgca
    11161 agacaaatgg agcgctgaat tgattagtaa cgttaccaat ggcaaaagta acgttactaa
    11221 tcaaggtggc gctcctattg gtaatcaaaa tgctaaaggt aacaaaggaa atagccgagc
    11281 ttctccgcca gtgggtaata aaaacgcttt gaaaacaggt gagtatgaaa caatattttt
    11341 tgacacgtta accgataatg agaaggacat ctattctagt ttgaatgatg atccttcttt
    11401 tgctttgtcc gaagaaatac gacttcttaa aataagacaa cttcgaatga tgaaaagaat
    11461 ccaacaagct gaagctggac taaatgatga agaagtcgaa cgattgcagc agctaagaaa
    11521 gattaaaact ccgattgaaa aaaatggtaa aaagctagaa atcaagcgtg aggttatgca
    11581 agatgtgcag attagcagaa aaaaacatcg caaaattgat gatattcttt caattgaaga
    11641 ttcattgact cggattagca accagttagc taaagccatc aagcaaatga atgaacttta
    11701 tatgaatgaa tacagaactg atttaattaa agctcagact gataagatcc aagctgagac
    11761 aaatgaaatt ggcggaaata attcaggtga agaaatagaa gaatggaaac aggcagtttt
    11821 aaatgccgca aacaaacggg cggtgaaaga aaatgaataa tgaatttatt ccttttgctg
    11881 atattggttc tgccattgat tattactatg ataaaccagt agctttttgc caagatattt
    11941 tgcatttgaa tcctgatgaa tggcaagaaa atgttttaaa tgatttagct gaattttcaa
    12001 aggtttctgt tcgttctggt caaggagttg gaaaaacagc attagaagca ggagcaatac
    12061 tttggttctt aacgtgtcga ccctacgcta aagtaatagc aacagctccg acaatgaagc
    12121 aactttacga tgtactttgg gcagaggtag ctaaatggtt aaatgatagc ttgatcaaaa
    12181 acttactgaa gtggacaaag accaaaattt atatggttgg tgattcagag cgttggtttg
    12241 ctacggctag aacagcgact aaaccagaaa gtatgcaagg ttttcacgag gaccatatgt
    12301 tgattgtggt agatgaagct tctggtgtgt ctgatccaat tatggaagct attcttggta
    12361 cgctatcagg ttttgataat aagctgttga tgtgtggaaa ccccaataat attgaaggtg
    12421 tcttttatga ttcccataat tcagaccgtg ataaatacag agttcataaa gtatcaagct
    12481 atgatagtaa acgtacaaac aaagacaata tagaaatgat tcttaaaaaa tatggaaaag
    12541 aaagtgatgt tgctcgtgtc cgtatttttg gagaatttcc gaaaggtgcg ttggattcat
    12601 ttatcagtct tgaaacagtt gaattggcta cagaaaaaca aattagtgat tctttagtca
    12661 ataaaacaaa ggttgctcat attggtgtcg acgtagctcg atatggtgat gattctacga
    12721 ttctctttcc tagaattgct accagggcat tggagtatga gaagtattca aaacgtagca
    12781 ccatggaaac aacaggatat gtcatcaaca tggccaagaa tctaatgagt caatatccga
    12841 gtattgataa agtgatgatt aaagtcgatg acactggtgt cggaggtggt gtaaccgacc
    12901 gcctagaaga acttatagaa gacaaacatt atccttttga ggtgtttgga gtgaataacg
    12961 gttcaacatc agaagacgat ttttacgata atttaggtac tcaactatgg ggaaacatca
    13021 aggaaatgtt agaagaaaat atgacagcaa atcttaacgg agaacagcct gttattgaat
    13081 tgccttctga tagttcgtta atcaaagaat taagtactcg caaattcaaa atgacaagta
    13141 gaagtcgtat acgtttagaa agtaaagatg atatgaaaaa gcgaaatatt ggtagtcccg
    13201 atattgctga cgcactggct ttagcgtttt atgagccacc aagtcactat caatttattc
    13261 aattttagga ggtgagcttt tgattagtac agtattatct ttagagagat ataagaagct
    13321 acgtgttaaa tatgcaacac aaattgaaga tggaatgttt gatccgaatg gttttataga
    13381 agatatgaaa ccattttttg ctgatcgcga aagaaaatat ctagcttata ctagcgaaaa
    13441 gaatgaaata gataatagac caaagcctaa cactgatatt gtaaaagtta ataataaact
    13501 tcatgctggt atgtattcaa ttgtcgtaga ccaagcagtc aatcatttta ctggtatacc
    13561 tatcaaatgg gattacgatg tatcggaaca aaaaagaaca ctcattcaaa gattaaaaga
    13621 taaattctta aaaaatgata tagaacttcc gacagttcca gaagctttta acaaattaac
    13681 aagcaatctt gattccatga gattcgcaat gcttgattct gaaactgcaa cttttcaagg
    13741 agcttgtgga gtagctttcc gattgctaga gcctgtggaa gaagatgatg gttggaagtt
    13801 aagagcaagt aatattgaac cttggagagc agaaagatat ggaaatgccg gaatctatat
    13861 caaagaaaag tatgactctt accagaaaaa attttttcaa gaaatgaaag ttatgacaag
    13921 aaataaaatt ctgacatatg cttgctatgg tgatttgaat tttgttacaa gcggaacgtt
    13981 caaaaaaatt gatgagaccg ataacccttt ggggacgatt accttggcag aatttaaaaa
    14041 taatacgaat cgttattgtg attttgaagt agctgaggaa attggagatg caattgatcg
    14101 tgctttgtct gatcaacaaa acgaaatcga acaatttaag cttgcgtaca tgcttgttac
    14161 tggcacaaca atgagcaaag gcactgctaa agaaatgatg aatcagctag ggattatcaa
    14221 cttaaaagcc cctactgcaa aagctgaata tgtcacaaaa aatttagcaa aagatttcaa
    14281 tgaatatcat atggatctat tgaaaaaaca gttttacacc atttgtaaag caatcgattt
    14341 taacgatgag gtgtttaaat caaacagttc tggagaagct cgcaagtggc aaatcattag
    14401 cctagaagcg aaaacaaata ctaaagagca atattttaga gaaggattga aagaatgcgc
    14461 agaaacaatt gctgcttttc ttaagtttca tgacaaagta gaaattgagc ctgaaaaaat
    14521 tatttttact ttctctagat ctttgccaac tgatttaagt tatttagctg aagctttacc
    14581 taaacttgca ccatacgtat ccaaacgaac tattcagagc caaattccat ttgtaacaga
    14641 tgttgattat gaaaatgaaa tgatggaatt ggaaagtgga agtgcttatc cagatagtga
    14701 atacaatttt ggcggaggtg gcaatagtga cgatagaaac gaagtattgg accaaacgtc
    14761 gagaactgga ggatcaagca aggctcaaac aagaaaatca gacacttaaa aaattaacta
    14821 gtgtatttcc tgaagcactg aaagagatac aagcaaaact attatcacaa gctgacttac
    14881 acaatatcac ttatccagaa atgatggagt tttacagtac aagtaatcag aaaaaatatc
    14941 gtgaatatgt ggaaaaaaat tataagtcat taaaaatgta tgatgcaaaa tacaaagagt
    15001 ttatcgatga attttttcca ccatttgact atgcaaaagt caatcgctta ttacaaatac
    15061 gatcagatgt atttaaaatt cttgcagaat atgcgatgga tgcagatgtg aatcaatatt
    15121 tttctgatcg cttagaggaa attcttcaaa gaacatattc ttctaatgct aatgtttttg
    15181 ttcaactttt aaacgttgat ataccaaatt atttaccaga aaatgaaatt aaacattatt
    15241 tgaactatcc atggtcagga aagacatttt caagaagact ttggggaaat atttcatcgc
    15301 ttgaacagaa actctctaat gctattgtaa aaagtgttgc tagtggagaa ggcgttatac
    15361 acgcattaaa caccatgaga ttagactcag aaatttgtga catgtttaag ttagaagaat
    15421 caaagtataa caaagcgata gaaaatctcg ttcgaacgga gtatgcaaaa tttgcacaag
    15481 atggtattga aaaatcatat ttagaaacag gtattgagga atacaacgta ttgactgcaa
    15541 aagatgagag agtttgccgg atttgtggag gaaaggcaag taagaatccc tataaactga
    15601 aagatgctgt cataggtgaa aatcgagcac ctttccatag tcgttgtaga tgtacggatg
    15661 ttcctaattt accaaaatta ggaaaggata ttgatgaaga atatgaccgt ttatttggcg
    15721 atttattaga tgagtttgca catgattctt ttggaattaa tttgaaacgg aggaagtaga
    15781 atgaaagatt tttttgaagc agtactaaca attaatgtaa atgctgatat tgcagaagcc
    15841 tacaaaacag ctattgagtc tgagaaccat cctaatggct tgagagacca ttggaatggc
    15901 aattatgcct acgtggttat tggcgatcaa actgttaatt atcaagataa tactccagtt
    15961 gataagaata ccgttaattt aacgattcaa ttattatctc attcattacc aaatttaaaa
    16021 gaaacagttg attggtatga aaagatggga tgtattgttg ttagaactga ctacaaagaa
    16081 ggaaagtcta gtaattaggc ttttttattt tgtccgaaat gacgttaaac tagcgcaata
    16141 ctgggcttgg ttgaatggtg gggcgcaact attaaaactc aaagcaatgc ggggcgtgaa
    16201 aacgaatcgt ggggcgaaag gagaatgagc atgaaacaca aatcattaat gccaatgaat
    16261 ttgcaatact ttgccgaagg tgacgatcaa aagttttctt ttgatgactt caaatctttt
    16321 gtggaatcca atgaagaagc tcaaaaattt attcagtcac aatctcaaag tgttgccgac
    16381 aaacaattgg aagcttggaa acaaaataac ttagataaaa ttaaacagga taccatcaag
    16441 gaatatgagg aatctaagaa aaacaagtca cctgagcaaa ttcaactgga aaaactacaa
    16501 gctgaatttg aagcagaaaa agcgttgcgt gtgacaagtg ataataaagc atttgttgca
    16561 gaacaaattg ctggattaga actagatgga gagttaaaag agtctatttc tcaatttatg
    16621 ctaaataatc ttgttagttc ggatacagat ttcactaaga atgctgttga aggttttaca
    16681 agtgttttga atgcaattaa tgagaaacat gcagatgcat taaaagaact acaaatgaag
    16741 tctgcatttg gtggaactca acaatcgaat aaccaggttc agcagaacaa tgaaacatta
    16801 acaaatccag aagaacaatt aggacaaatt cttcaacaat ttaactagga gagtgaaaaa
    16861 ttatgaaaaa aacatcttta aataatttag agtatttgga tatttcacca gcgattaatg
    16921 ctatgcaagt accaaataca ccttttttaa gctatttact tggtgctgga aaaacagagc
    16981 cagcaaactc gacagaaatt aaatggcgtg aatatgatat caacaacgat gattcttctg
    17041 aaaaacttga gggcggagaa tatccagatg ctgaatcagg tcgaacttgg tttaacaact
    17101 atactgaaat ttttagaaaa tcaacctctg tatctggtac attagatgct attaatgtga
    17161 atggagtcgg aaatgaatta actaatcaag tagcactacg tggtatggaa atgaaaattg
    17221 atttgaaccg aaaattgatt actggtgtaa aagctgatga aaatggttct aaaggtcgtc
    17281 gaatgaatgg gattttgaac ttgatcaatt cagcaaataa ggcagaaaca gccactgcgg
    17341 gtgcagtaac aagaaaagat atcgatactt tatttaaatt gatgtatgaa aaaggttata
    17401 tgggagaaaa actatgcttg atttctccag atatgcagga gttaatgact gatgagttag
    17461 atggaaaatc aacaaaaatt gttcagttcg gggaaagagt aacttttgga ttgcaaattg
    17521 gaaatatcgt gtctaattac ggtacaggta ttgctctact agaaccatca ttgccaaaag
    17581 gaacaattgc cgcaatcgat actaattatg tgaaactacg tccattacgt gaatggagag
    17641 cagaagaact tgcaaaaaca acggattcta gacgtatcgg tcttgtaggt gaatactctc
    17701 ttgaatacaa tgcttcaaac tctggggcaa ttttaaattt aaagtctgaa taaaagggag
    17761 ttagtactcc cttttttgat aggaggaatt acagtggtaa aaaaagatga aactaaaaaa
    17821 gatgaagtcg tgaaatatag agtaggtaaa actaaaaatt ttgttgggtt tgttcatcct
    17881 aaaactcgta gatttatcac agcagattca aataacgaat ttatcatttc tatagatgat
    17941 aaagaagcaa ttgcaatttt ggaagatgca attgatgtta atgaaattta ggaagtgatc
    18001 tgatggatga atcgctaaaa acggaaatca ttgagtctac aaaagaagat tttccagatt
    18061 tgagtgaaga acgcataact aatttattag aaataatttt gctagaaatt gaatcataca
    18121 acacttgtaa aaatgatatt tcatgggaaa agttaaaaag cgtgattagt gaagtgttgt
    18181 ataaaataat aaaaaatgaa tcagaaaaaa cagtatcttc agttagacgt ggcgatacaa
    18241 cgattagtta tgcttcaaca acaaatgatg ttagtgagtt acttctaggc tatggtgatt
    18301 tgatacgaag agttattggt tgtggaggat tggagttttt ttaatgaatg aagcagatat
    18361 tttagaaact acttatgaag atagttgtat tattgaaaga ctgacggaca ttgaagattc
    18421 taatacaaat attactattc aagattacaa aaaagtatat gataatccta tttcttgcgc
    18481 tctttcacaa ggtcaaatcg atggactagc agtcatagaa gatggagaga tggtaaatgt
    18541 ttcaactgac acatataaat tatttgttca tcctaagatt aaactaaaaa aaggagatcg
    18601 aataacaata actcaaaagg ccagtggctt aattttttct ctatttgcta ctaagccttt
    18661 ttactatcct agtcattgtg aagtgaattt gataggaagt gagaaaaatg ggtaatctca
    18721 aatatgaatc aaatgcagaa aagatcattg agaattttaa aaatatgact gtaattgctc
    18781 aaaaagaagg aatatccttc gttaacgatt caatgaataa agtcgttagt ttagctaaac
    18841 cattaactcc tgtaaaatca ggtaatttaa ggcgaggata tcgtgtagta aaagctagaa
    18901 aactatcaag cggtcgaatt gttggagaag ttataaacaa tgaacattat tttaaatatg
    18961 tagaagaagg tcataggact aaaaatggtg gctttgtcaa agggaaattc atgttaactc
    19021 gtgcaacgaa tcttgcaaat atgtcttata ttcctcgaag atttaaacaa atggcaataa
    19081 aaatcgttaa gaaaggaaag taacatgaaa gatgaaatca ttgctgcaat cagtagcaaa
    19141 ttaaaagaaa tctatcctga tgggacaata tatcttgatt cagttatgca gtcaactaaa
    19201 gatttttatt tcgttttatc agtaatggaa tctggaactg aaaatgtagg aattgatatt
    19261 caaaatgttt ctttcttaat tgatattgca ttgattgata ataaccctaa tagaaattta
    19321 attaatgaat tagtctcacg ttgtgggact ttttttaata cgattacaat cgatgagcaa
    19381 atattatttc caaaagccta tttacctgat gaagcagatg gtgttcaaca tattcgtttt
    19441 acattagaat ttccacaata tattgaatgg agtgaaagat aaatgggaga aaaaagaagt
    19501 aaaaccggaa ttatttctgt cgaaaaacct acttggtttc ctttggaaga tgagacaggg
    19561 aattttccgg tatatggtac tgcgttgcca atgggaacag cagtaagtat caaaccaaca
    19621 gctaattatg aaacaacaca agattatggg gattcagtgg ttcaagatca atttacagct
    19681 tttggtggtg cagaagttga actggaagca aatggatata ctcataaagt tttatctaca
    19741 attactggag ggaaaattgt taaaggtggt gccttgcgtt ctggagaaga tattgcgcaa
    19801 gatggtgcgt ttgcatatag acgtaaaaaa tcgaatggga aataccgtta tacggtcttt
    19861 tataaaggac agtttgctct agattcagat gaaacatcaa cgattgaagg aagtaaagta
    19921 agttttactc atccaacttg gaagggttca tttgtagatg taccaggact tggatatatg
    19981 tattcagttg atgaagatga tgaaggcgtg gataaagcaa tgatcggaaa ttggtttaca
    20041 aaagtagcaa ttccaattga agaagcagaa ctttcaggag gtacagaata atgtcaaaat
    20101 atcaaacaac gattaaatta acgaaaaaag acgaagaagg taaatatgag caagtacaat
    20161 ttaaatctgc tgaattttta ccaggaacag ttgtagaaga tgctgcagga gttatggaag
    20221 aaatgcaaac agcgactgat aagcaatcag ttaaaaaagc tttaagtcgt gcttattcat
    20281 ttattgcaga tactttattt gagggacaat ttactggtga agattattgc aaaggaattg
    20341 atgctcgtga gattgcctca ttgacaggaa aattattgaa gtctgttact gcaggttttg
    20401 atgaaactta tacagagacg aaaaaaaagt aagtgaggcg ctcaagtcac cttcatttaa
    20461 gtattcgatt acttaccgag aattagatat aaagacgcaa ttactcgaag caggttggac
    20521 gttaccagaa attgaacgta ctgacttgga tgagttaatg cgtctttttg cttttagaga
    20581 tgcggtaaaa gagcatgaag atgttgagta ctatgataat ttcactcaat tttaggaggt
    20641 gatactttga ataacgatga cctaattctg aagatgatac tagatgaatc tggttttact
    20701 gccggtatga ataatgctgt caaaaagttg aactcttttg atgaaacaat cgaaagaaaa
    20761 agtagaaata gtggcagttc attaggtagt atttggaaaa tatttgctgg aagttttcta
    20821 gctagcggag taactagaat cgtaggagct ggctttgatc taatcaaggg ttccataagt
    20881 ggagcggttg atcgagtaga tacgatgaat aacgctctac gaaatttcca aaatatggga
    20941 tttagtaatt cagaaattat gaagaatatc ggaaagaatg ggcttttatc tcaaggtatt
    21001 caaggacttc ctactgcttt gaatgatgcg ataagtcatg ttcaacttct tgcttcttct
    21061 acaggtgatt taactcgttc aactcaaata tttaaagctt taaatgatgg aattcttggt
    21121 ttcggtggtt caactgatca agttaatgaa gctgttattc aattatctca aagtttctca
    21181 aacggaaaag tagacgcaca aacttggaac tcaatgatca acgctcaact tggacctact
    21241 ctttctgcta ttgctaaaaa gatgggaatt acaatgggag atctgaaaga aggtttatct
    21301 caaggtaaga tttctgttga agaattccaa aatcaattaa tagaaatgga taccaaaggt
    21361 ggcggaggac ttaaatcatt aagtcaaatc gctaaagatt ctactaaggg aattaaaacc
    21421 tctatacaaa atgcaaaaac agctgttaca cgtggtgttg gtgaggtaat agaaggatta
    21481 aataaagcat tagtagattc agatttaggt ggatttaagg gaattattga taaagtgggc
    21541 agttcaatgg aatcgttctt aaaagtaatt gctgcaaata ttcctacagc agtatcattt
    21601 ttaagtaatt tatttaacga aattcaaaaa tttggttctg ctttgaaatt catgatgccc
    21661 tttcttgttc ctgcagctac cgcttttgga gcgttcatgt ttcaacttaa aggtatacca
    21721 gcaataataa aaagcttcaa taattttaag aatgccataa ttggcgttgg aaattcatta
    21781 aagattatgg gggcaatagc tgctgctaat ccatttgttt taattattgg tgcagtcgtt
    21841 ggtgcaatag ctgttttcgg ttactttatg gcaaccaatg aagagcttag aaacaaggtt
    21901 atatctattt ggaatgatgt gaaagattcc gtaattggtg tattaaaaaa tataaaggac
    21961 tggggaattg atacttggaa ttctgctaaa gaaatggcat caaatgcagt tgagggtgtc
    22021 aaagatgctt ggtcaggaac aaaggaatgg ttttctaaca cctggaaagg tattaaggat
    22081 ggggccacag gtttatttga taaaacagta gagacttcaa gaaatgcagt cgatagtgta
    22141 aaaaatgcat ggtctaatat gaagaaatgg ttttctgata cttggcaaag tataaaagat
    22201 tcagccagag aaaaatggaa tggaatcaaa ggttctatta tgtcagttgc tggtccattg
    22261 attacaggta taaagaatgc atttttacat gttacttttt atctagagac cttatggaat
    22321 aatttagttg agattggtaa aaacgtcttt gagattttaa aaaatgttat acttgcgccg
    22381 gttttgttta ttacctccat gatttctggt ggatgggaag aaacaaaaaa taatatgatt
    22441 gctgtttgga ataatattaa agaaagtgcc ataaatattt gggaatctat aaagaatata
    22501 tttgtaagtt attttacaaa tatttatttt gctgcactca atatttggac agggttcaag
    22561 cttacattga taaatatttg gaatgaagtg gtaaatcaag ctaaatcgat ttggttaaac
    22621 ataaagtatt tttttattaa tctttggatt gatataaaat attttgcaat tcagaaatgg
    22681 attgaattga agttttctat tattcaaact tggatc
//
LOCUS       DAZKNH010000040        22653 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000040 DAZKNH010000000
VERSION     DAZKNH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.040"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..350
                     /locus_tag="ACG8MG_001438"
     CDS             <1..350
                     /locus_tag="ACG8MG_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFQ0375489.1"
                     /translation="IFAHLPIPGSILGILFLFLAFEMHLVDPEKIGATGDFLLNNLAI
                     LFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVAAGKTAEGVEALLGYVRKKKAE
                     RVSVNEMEENQID"
     gene            343..1044
                     /locus_tag="ACG8MG_001439"
     CDS             343..1044
                     /locus_tag="ACG8MG_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFQ0375490.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            1044..2630
                     /locus_tag="ACG8MG_001440"
     CDS             1044..2630
                     /locus_tag="ACG8MG_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0375491.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2649..2870
                     /locus_tag="ACG8MG_001441"
     CDS             2649..2870
                     /locus_tag="ACG8MG_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFQ0375492.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2867..3628
                     /locus_tag="ACG8MG_001442"
     CDS             2867..3628
                     /locus_tag="ACG8MG_001442"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFQ0375493.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            4008..4517
                     /locus_tag="ACG8MG_001443"
     CDS             4008..4517
                     /locus_tag="ACG8MG_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0375494.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4592..5131)
                     /locus_tag="ACG8MG_001444"
     CDS             complement(4592..5131)
                     /locus_tag="ACG8MG_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFQ0375495.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(5271..5882)
                     /gene="rpsD"
                     /locus_tag="ACG8MG_001445"
     CDS             complement(5271..5882)
                     /gene="rpsD"
                     /locus_tag="ACG8MG_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFQ0375496.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            6144..6995
                     /locus_tag="ACG8MG_001446"
     CDS             6144..6995
                     /locus_tag="ACG8MG_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFQ0375497.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            7023..7658
                     /locus_tag="ACG8MG_001447"
     CDS             7023..7658
                     /locus_tag="ACG8MG_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFQ0375498.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7678..8451
                     /locus_tag="ACG8MG_001448"
     CDS             7678..8451
                     /locus_tag="ACG8MG_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0375499.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8859..9656
                     /locus_tag="ACG8MG_001449"
     CDS             8859..9656
                     /locus_tag="ACG8MG_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFQ0375500.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9634..10047
                     /locus_tag="ACG8MG_001450"
     CDS             9634..10047
                     /locus_tag="ACG8MG_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0375501.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            10031..12676
                     /gene="mutS"
                     /locus_tag="ACG8MG_001451"
     CDS             10031..12676
                     /gene="mutS"
                     /locus_tag="ACG8MG_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0375502.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12694..14802
                     /gene="mutL"
                     /locus_tag="ACG8MG_001452"
     CDS             12694..14802
                     /gene="mutL"
                     /locus_tag="ACG8MG_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFQ0375503.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14824..15384
                     /locus_tag="ACG8MG_001453"
     CDS             14824..15384
                     /locus_tag="ACG8MG_001453"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFQ0375504.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(15469..17457)
                     /gene="treP"
                     /locus_tag="ACG8MG_001454"
     CDS             complement(15469..17457)
                     /gene="treP"
                     /locus_tag="ACG8MG_001454"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFQ0375505.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17680..18393
                     /gene="treR"
                     /locus_tag="ACG8MG_001455"
     CDS             17680..18393
                     /gene="treR"
                     /locus_tag="ACG8MG_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFQ0375506.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            18470..18916
                     /gene="msrB"
                     /locus_tag="ACG8MG_001456"
     CDS             18470..18916
                     /gene="msrB"
                     /locus_tag="ACG8MG_001456"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFQ0375507.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            19086..19688
                     /gene="ruvA"
                     /locus_tag="ACG8MG_001457"
     CDS             19086..19688
                     /gene="ruvA"
                     /locus_tag="ACG8MG_001457"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFQ0375508.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19701..20702
                     /gene="ruvB"
                     /locus_tag="ACG8MG_001458"
     CDS             19701..20702
                     /gene="ruvB"
                     /locus_tag="ACG8MG_001458"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFQ0375509.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20731..21309
                     /locus_tag="ACG8MG_001459"
     CDS             20731..21309
                     /locus_tag="ACG8MG_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0375510.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            21361..21843
                     /locus_tag="ACG8MG_001460"
     CDS             21361..21843
                     /locus_tag="ACG8MG_001460"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFQ0375511.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            21960..22334
                     /locus_tag="ACG8MG_001461"
     CDS             21960..22334
                     /locus_tag="ACG8MG_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375512.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7373 a         4062 c         4905 g         6313 t
ORIGIN      
        1 ggatttttgc tcatttacct attcctggaa gtatcttagg tattcttttt ttatttttag
       61 catttgaaat gcatcttgtt gatcctgaaa aaatcggagc aactggtgat tttttattga
      121 ataatctagc cattttattt gttcctgctg gtgtaggttt attggaatat tttgatgata
      181 ttgcttcgat atggcctgtt ttactgggag cagtcgtcgt gtgttccatc gtcacgatgg
      241 ttgcagcggg aaaaacggca gaaggtgtag aagctttgct gggctatgtc cgtaagaaaa
      301 aagcagaaag ggtatctgta aacgaaatgg aggagaatca aattgactga gttttttgtg
      361 aatccattat ttggcttaag tttgacttta ggagtgtacg tattatctga caaatttttg
      421 cggaaatata aactgcctgt tttgaatcct ctcgtattat cgattgttgt tattatctta
      481 tttttgcatt tttctgggat cagctataaa gactataata tcgggggcaa ttttttgact
      541 atgctgatta ctcctgcgac agtggcactt gccatccctc tttataaaaa cttccgttta
      601 ctgaaagaga atttttatcc tgtaatggct gcgattttag caggaatagt cgcaaattgc
      661 ttggttagtg tagggttcgg ttacttcttc tctcttcata aagagatggt gatctcatta
      721 ctacctaaat ctgtaacgac ggctatttct gtcgatttat ctcatacgat gggcggggtg
      781 aatgccgtga cacttgctat tgttgtctct acaggtattt ttggttcgct gatcgccact
      841 catattttcc gcctattcaa gatcgaaagt ccagttgctc gcggtgtggc gttaggaagt
      901 acttctcatg ctattggtac agcaaaagca atcgagattg gtgaaataga aggaatcatt
      961 tccggacttg cgatatgcgt caacggaata ttgaccgttt tattgctacc gttattcttt
     1021 cagccttttg ctggcttgtt ttgatgcgaa aaccactatt ttatgctaca ataaccaaaa
     1081 cgttcgagca taaaaaagga gggaaagacg tgtatttgtc ttccgagtat cttaaacaat
     1141 ttgataaaga tctatattat aagattctcc tcctagaaag ttttgaggat caagcttggc
     1201 ataccgctgc acaattagcg caagttgttc agctggatgc ccgcagcgtg tcgaaatacc
     1261 tgaacgagct gtctaaaaac tatcagcagt ttagcggtaa gacacaccct ttgtttacaa
     1321 aaaatcaccg ttctggttat aacttttatg atacgctcga ttcaattgaa catgagcgtt
     1381 ttttgatcta tctcgtgcaa agtacgctga aattccagtt gctgcacgac attttttttg
     1441 aagaattcca tacaatgtac caatttgcgc aaaagcatta cattagtgaa tctactgctc
     1501 atcgaaaaat caatgaatgg aaacagcaac tgcaaactta cggaattcgg ctgcaacgag
     1561 gtacatatat cgctcaaggc gaagaagaaa tcattcgact ttacttgcat atgacattct
     1621 ggcagttgtt tcgagggaaa atctggccat ttgaaacgat ttctcaaatg gacgtaaaga
     1681 atatggcaga acatatcatg gcttttttta atgttcgtct gaacgagatc aaaaaaagac
     1741 gattggaata tatgctaggt gccttttttt taagaaaatc acagaagcat tatgttgtat
     1801 tgaatgagaa aaaaagacgg ctgatttctg acaatctgtt gttccagcga ttttgccagg
     1861 tcatggagcc ggtatttcct aattattttc aagtagaaga cgagttaggc gcattatttc
     1921 ttgtactgat gactcgtgaa gaatattata gtgatcctaa gatacggaag aaaatttttg
     1981 attttcatca acaagcaaaa acaccgccct ttacagccct ttcagaagca aaagccgctc
     2041 tttctcttta tcaggaagag caggggctgc cagcagaaaa tctaaccttc gaggcagaaa
     2101 actatttatt cagcagtcat tttttcgcct atctttttcc taatgcgaaa gaaacgattg
     2161 atggcaatag tagtgatttt attaatcatt tagttattga aaacaaggaa ttgaaacaat
     2221 ggctcgtaca tttttttgaa agtagacaca aacatcccaa tcatctggca tttaaaaatc
     2281 atgcgttttt gatggggcgg tatctgactg tgtttaaaac attaggagcc tttactccac
     2341 agcttccaaa gatcacgatc cttttaatga cagatttccc attatttgaa gagcaactgt
     2401 tagaggaggg attacggaca tttttccgta atgagtatca gctgattttt ctgccaacag
     2461 attatcgggg gagagaagtt gatcttctga tttcgacaag taaagtccac cgcaaaccat
     2521 gggcggatct tgactatttt attgtgactg aagaactaaa attaattgat tacatccaac
     2581 tatctcaaaa atttgaaatg attcaaaaac aaaaactatt aaaacaataa caaagaagga
     2641 gaaaaaagat gacatttgaa gaaattttac cagaattgaa aaaaggtgca aagattattc
     2701 gtaaaggatg gagtggattt gaactttatg ttgttctcgt atccgatgat gaatttgatg
     2761 gctgtccggt gacgccgtac ttattgatca aaacaagtga cgaaggtttt tccagcttcg
     2821 ctcctacagt ttgtgatatt ttagcagatg attgggcgat cgtagaatga aaaaccagta
     2881 tccagatcta gctaaacagg ttgtatttat cacaggagct gcttctggaa ttggtgctgc
     2941 gcaagcccga gcgtttcttg aacaagatgc ttttgtattt ggattagatc aaaagttcgg
     3001 taaaatggaa gaaatttctt tgcgatttcc tgaaactttt gcctacgcac taggtgatgt
     3061 tcgacaaatg aaagaactcc aacaagcggt atcgaaatgc caaaatcatt ttggtgaagt
     3121 tacgatttta ctgaatacag cggggatatt ggatgcctac aaacccttga tggaaactga
     3181 cgaatcgctg tgggatctga tttatgagac gaatgtgaaa agcatgtatc agctgacaaa
     3241 aatcatcctt cctgatatga tccgccaaaa aagcggaacg atcatcaata tggcttcgat
     3301 tgctggcttg attgctggtg gaggtgggat cgcttataca agcgcgaaac atgcgattgt
     3361 cggatttacc aaacaactcg ctctggatta cgcaggacag ggtatccgag taaaaggaat
     3421 tgctccagga gccatccaaa caccaatgaa tgcggctgat tttgaaggga aaggtgagat
     3481 ggcagagtgg gtggcaaatg aaacaccagt caagcgatgg gcacagccgg aagaagtagc
     3541 tgagctgacg ttatttcttg cctctcctca agcaagctac atacaaggaa cgatcatccc
     3601 aatcgacggc ggatggttgt taaaataacc ttgtgttttt ttcaagcttc tgtatgataa
     3661 aactaaagag agaaacaacg gttgatgata aaaaaagtcg atgactgttc ccgacattcg
     3721 atgccgaaca gccagttcaa aaaattcttt ttaataatcg ttaatattcg aaactaagcg
     3781 gcttttcacg acctcttgat ataaacgata ttcaaaagct tgcaactgcg gtaacaagtc
     3841 cagccaaatg aaaaatataa ggagctattt ttatttgacc attcttatta cttgttgcag
     3901 ggcagctttt tcactacctt ctgcgacact tcagttggaa ttttcttccc atcctttggt
     3961 cgcggtcatt accaagataa aattatcttg caaaggagaa atagaacatg gaaaaaaata
     4021 atacccgttc agccaaacgc tggacaacga cagatgtgac tcggatggcg ctagtcactg
     4081 gtttatatgt ggcagtgacg ctggttttgt ctgtctttag ctttggagtg attcagattc
     4141 gtctctcgga gatgttcaac tatctgtcct tgtataacaa aagatacatc gtcgcggtaa
     4201 cagcgggagt agctttggct aatattgctt ctcctcttgg actgatagat gttgcagtag
     4261 gaagcgtcag cacgctgctt gtattgctta tcaactataa aatcactagt cgcatcaaaa
     4321 acatgaaaat caaaatggcc gtgacagcat gcgtatttgc tttttccatg tttacagtag
     4381 ctggtcagct aacgatttta tatcaactac cttttttctt gaattggtgg gtgattgctt
     4441 taggagaatt ggcatcaatg attgtcggtg ggataattat ttattggatc ggtaaaaaag
     4501 tggatttgtc aaaataaaga tctgtttaat ttaaaatagg gcagcatgga agagactgaa
     4561 tgttgagtct tcttccatgc tgcttttttg attaatttgt atgaagaaat cgatttagcc
     4621 gttttttcat caagataccg atataagctg ctccagttgc cttgattgct tccggcagta
     4681 aaaacggcag tagtccactg gcaaatgcag ttgtaaaagt aagatcacca cttattttca
     4741 gccagatcgt tccgcagatc aatgcagcaa gagagcccat tagattggca aagattgcat
     4801 ataagtaatg agcatccgtt ctttcgatca tccaaccagt tatccacgtt tgtaagagaa
     4861 atccaatcaa atatccgcca gttggcccaa ataaaacagc tatacctcca gtcatccctg
     4921 aaaatacagg aagaccaatc aagccaagta aaagataaac tccaacagat atcaatccta
     4981 gttttcttcc gagaaaagtt gctgttaatc caatagcaaa tgtttgccct gtcaaaggaa
     5041 ccaaaccaaa aggaatggtc atctgagaaa ggacagaaat aatcgcggca aattccgcag
     5101 caagtatcaa ttctttcaat gttcggttca taacgccctc ttttcgttaa cttaatattt
     5161 ttatttggtt aacgaaattg taaaacaaag tgattacttt gtaaatagaa aatataaaaa
     5221 aagaccagct atccaataga tagcggtctt tttcaagaaa gcttttgaaa ttataagctt
     5281 tggttgtagt attcaacgac aagagcttcg tcgatttctg gatatagttc gtcacgttct
     5341 ggtaaacgag tcaatgaacc ttccaatttt tcagcgtcaa agcttacgaa tgctgggcgt
     5401 ccaacagttg cttctacagc ttcttttact gttacgatgt tttgtgattt ttcacgaact
     5461 gagatcactt gacccacttc tacgtggtat gaagggatat caacgcgttt gccatcaact
     5521 aaaatgtgac catggttaac taattgacgt gcttgacgac gagtcgttgc aagtcctaaa
     5581 cggtaaacaa cattgtctaa acgttgttct agtaagatca tgaagttaac accatgttta
     5641 ccttctttga ttttgctagc ttttacgaac aagttacgga attgacgttc gttcattccg
     5701 tacatgtgac gaagtttttg tttttcagtt aattgcatgc cgtattctga ttgtttgcca
     5761 cggctattag gtccatgttg acctggtgcg taagggcgac gtgctaattc ttttcctgtg
     5821 cctgacaatg agatgcctaa gcgacgagaa attttccatg atggtcctgt ataacgagac
     5881 attaaaattt cctccaatga atataagttt ttggagtaaa ataatccgtt gaaaaattca
     5941 taatcatata gtttgttctt caatcttcac ctttgcagcc gcggttacac aatcgaaccc
     6001 gagacggggc aacaaactag ggatgagtta ttatttttct gctgcattat tttacacaaa
     6061 ttacagtata tgatagatca tccacgaatg tcaagataat tagtacataa aaacgcttta
     6121 caaacctagg ctgtctttat aaaatgagag ggtatgtgca aatggaaaaa agcgaaggaa
     6181 gtttaaggag aaaatcaatg aatccggtat caccattatt tgagaaaatc gatgcttcaa
     6241 ttgaaaagaa ggcgaatctt tttgaaaaca gcttttcacg ttacgctgtt cgagcggttc
     6301 tcgcctgttt gtttttgact ttaggaacgg ctgtggcatt tgcgatcgcg atgaaggggg
     6361 aagatatcgc gcatggtcta ggaaagattt tatacgcatt tatgttcagc tggggtctgg
     6421 tcatgattct atacatgaac gccgaattag gaacatcgaa tatgctttat atgaccgtgg
     6481 gtgtttttcg gcagagactt gctttaagta aggcagccaa gattctattt gcctgtattt
     6541 ttttcaatat gattggcggt atattatgtg gatttctaat ttctatgact acgccatttc
     6601 aagatttaac gttggacaac tttatgttag aatcaatagc agtaaaacta gcaaaacctt
     6661 cagcaacgat tttggtggaa gggatgtttg cgaatatcgt tgtcaatacc gctgtactga
     6721 tcagcatgcg gatgaaagag gatgtcggaa aagtgatcac agtcgtattc atcattttca
     6781 tttttgcttt tttaggatat gaacacgtga tagcgaattt tcctgccttt acgcttactt
     6841 actttgcttc tcacggacag atggatggaa tgacagtcag caatgtgtta cataatttgt
     6901 tttttgcttt ggcaggaaat tatattggtg gcggtttagt aatgggactg ggatatgctt
     6961 ggctgaatca gtcaaaatca tcctatgtgg actaaaaagt cgttcattaa aggggaagtg
     7021 ccatggaagt tttagttttt ccaaaaaatg aggaaaaagc gttacaaatg gaaaagtaca
     7081 tgaaaaatct tttttccttt gctggagtac ctaagccgga acgcgctctt ttacaacaac
     7141 cgttactgga tgagagcaaa aatttatcta tttctgaagt tttagctttt gttcattatt
     7201 attatgaaca aatcgaaaga gagtatcagt atgtagcgat cgatttagtc cagaaaaaca
     7261 tcaaacggct tacatttgaa gacttagtcg tattgttgcc actagttcag aaaaaagaat
     7321 ggtgggacag catcgactct tggagaaaag tatacagcac atggtgtaaa gcctacccag
     7381 agcagcgtga agaagtcttt caattgtttg aaaaaaagga agatttttgg ctacgtcgaa
     7441 tagcaatcac gttgcaatta ggttataaac agcaaacaga cacaggacta ttgatccgag
     7501 caatagacga agatcgtcaa acaaaggaat tctttatcca aaaagcaatt ggttgggcat
     7561 tacgcgatta tagtaagacc gatgccgatt gggtaaaaag acagctgaca acagatttga
     7621 gtaagttagc tgttcgagag gcaagtaagt atttataaga aagcgagacg acaagagatg
     7681 agacgcaaat tatttgcttt tgatattgat ggaacgttgt tagattcaaa caaacaagct
     7741 ttagatagca caagagaagc attggaaaaa ttacgcaaag ccggtcatct ggtcacgatt
     7801 gctacagggc ggagccgatt tcatgcgcaa gagatcattc gcgatcttga ttttacgaac
     7861 tacattttat gtaatggagc ggccggtttt ttagaccatg aacaagtata taagaattta
     7921 ctagatcgcg accagttgga tgcgttcgtt caagaagcgc atcatgagca gatcgataca
     7981 gcttttgtca gcttagacaa tatcaagcgt tcttcttcca atgatgtaac ggtgatggaa
     8041 gaagcgatgc attctttcgg tgctgtcttg cctgaattag atcagttttt tgcagaggaa
     8101 caagaaatat accaagcgtt agcatttttc gatcaaacat atgatgggaa gttctcttca
     8161 tatgataaaa ttcgatttgt ccgctggcat gagaacagtg tagatgtcgt acctaaaaat
     8221 ggttctaaag cagcgacaat tctgcactta gcagaacaag taggcatccc tcatgaagac
     8281 atcatcagct ttggcgatgg acaaaacgac cgtgaaatgt tgcgcatgtc aggaattggg
     8341 atagctatgg ggaatgctgt gccagaagta caagcagaag ctaagatggt cactgataca
     8401 aacgatcagg atggaatttg gaaggcattg aaagagttaa gcgtgattta aaagaggttg
     8461 tgaaaaagct gttctgcatc aagtaataag cccagccaaa caaaaatagc tcctcctatt
     8521 tttcgttttt ctgggcttat taccgcagat gcaagctttc gaacaccgtt taaggaagag
     8581 gtcgtgacag aagtggtcag tttcaagaaa taagaaggga tttacgaaaa ttgcttctca
     8641 aatttttgta aaattacggc ttatttctga agaagtagat gaaacaacgt ttattcggga
     8701 aatgggactg tgacaagatg tttgtcatag tcccattttt ttattttatt tttcaaaact
     8761 aagaaatacc aggaaagagt tccttttgtt tgaccttttc tttcttattg aaaacaacta
     8821 taatatagag tgatgttaaa agagaagagg aatttagtgt gcgggtattg tttattggtg
     8881 atgtagtagg ttcaatggga cgagagatga ttactgaata tcttcctaga ttgaagaaga
     8941 agtaccgtcc gcaagtaacg attgtaaatg gtgagaatgc cgcttctgga cgtggaatca
     9001 cagaaaaaat ctataaaaaa tttttgcaag acggcgtaga tgtcgtgacg atggggaatc
     9061 atacgtggga caaccgcgga atctttgagt ttgtaaatga agctaaaaag atggttcgtc
     9121 cggcaaattt tccggaaggg acgcctggac aagggatggt ctttgtcaaa gtgaaccaag
     9181 tcgaactggc agtgatcaac atgcaggctc gttcttttat ggtggacttg gatgatcctt
     9241 ttcgtaaaat gaatgagctg gtagaagaag ccagaaaacg tacgccgatc atttttgtag
     9301 attttcatgg agaaacaaca agtgaaaaac aagcaatggg ctggttttta gatgggaaag
     9361 tttcagcagt ggtcggtaca catacacacg ttcagacaaa tgatgcgcga attttgccaa
     9421 gaggaacagc gtatctgaca gacgttggga tgacaggtcc ctatgatggt attttgggta
     9481 tgcgcagaga acctgtaatc gaaaagtttc tgactgcatt accaaaacgt tttgaagttg
     9541 tggaacaagg ccgatcgatt ttaagcggct gtatcttgga attggatgat acgacaggtc
     9601 atgcaaaaga aatccagctg attcagatca acgatgatcg accgttcatg gagtagaaag
     9661 agaggcagtt ttttgcagca ggagccggaa attcaaaaag aagtaaggaa actgacaaaa
     9721 cttttaagag agaatgaaac gatcattcgc tacaaagaac ttgaagaaaa gatccagcaa
     9781 aaccagtatt tagctgagtt gagagaaaaa atcaaacaag cgcaaaaaga tgctgttcat
     9841 tttgcccact atgacaaacc agcagcagaa aaagaagcaa tcaaacaagc ggatcaattc
     9901 atgcaagaat ttgatcagca tccgcttgtt gtagcttata gaaaacagct gttggaagca
     9961 gatgatctgc ttcatcattt aacaactatg atacaagaag aaatcaatgg acaaatagag
    10021 gaggaaaaac atgcctcaaa aaactaaaaa tactcctatg atggaacaat atctaagcat
    10081 caaggcccaa taccaagatg cttttttgtt ctatcgttta ggagactttt atgaactatt
    10141 ttacgaagat gcgatcaaag cgtcacagct tttggaatta acgttgacga gcagaaatcg
    10201 aaatgcagaa gaacctattc ctatgtgcgg tgtacctcac catgcagcac aaggatatat
    10261 cgatgccttg attgaaaaag gctataaagt agcgatttgc gagcaggtag aagatccaaa
    10321 gacgactaaa gggatggtca agcgcgaagt gatccagcta gtcactcctg gtacagtcat
    10381 ggacagcaaa ggattgtcgg caaaagacaa caatttttta acagctgtag ttagtcaagg
    10441 aaatgaattt ggctttgctt atgccgattt aagtacggga gaattgaaga cggctcggtt
    10501 gcatgatgaa gaagctgttt taaatgaagc ctctgcattg cagacaaaag aactcgtatt
    10561 aggcagtgag atcactgata cccttcgtga acagctaagc gggcgtttag ggctagtctt
    10621 ttctcaacaa aatgagatgg aagaaaacgc ggaatttagc tttttgacaa gcgaactagt
    10681 cgatccgctg gaaaaagaag caaccggcaa actattgact tatttatcgg tgacacaaaa
    10741 gcggagttta gctcatatcc aaaaagcagt agaataccaa ccagatcatt ttttgaaaat
    10801 ggattattat tcaaaattca acctagaatt aagccaatct attcggactg ggaaaaagca
    10861 cggcactctt ctatggttgt tagatgaaac aaaaacagca atgggtggcc gtttactaaa
    10921 acaatggcta gaccgtccgc ttatccaggc aaaacaaatt aaagcaagac aagaaatggt
    10981 cgcttctttg ctagacagct attttgaacg tatcgatttg cagtcagcat taacgaaagt
    11041 ctatgatctg gaacgcctag caggtcgagt agctttcggt aatgtcaatg ggcgagactt
    11101 gatccagctg aaaacttctt tagaacaagt gccaatgatc agagggttga ttgaaggaat
    11161 cgatcaaggt caatggcaaa gtctgctttc agaaatgcag ccgatggacc atctcgtcca
    11221 attgatcgac caagctatcc aagatgatcc gccgttacaa ataacggaag gaaacatcat
    11281 caaagatgga ttcaatgagc agctcgatat gtaccgttct gctatgagaa acggaaagaa
    11341 atggttggca gaacttgaag caaaagagcg tcaagaaaca ggaatcaaga acttaaaaat
    11401 cggatttaac cgcgttttcg gttattatat agaaattaca aaagcaaatc ttggaaatgc
    11461 agaactggag aagtatgaac gaaaacaaac tttagcaaat gcagagcgtt tcatcacacc
    11521 agaactaaaa gaattggaga cacaaatcct cgaagcagaa gaaaaatctg ttgatttaga
    11581 atatcagttg tttttagctg tgagagaaga agtcaaaaag gctatccagc cgctacaagt
    11641 acttgcaaaa gcaatcagtg cagtagatgt gctacaaagt tttgcaacga tcagcgaacg
    11701 ctatcagtat gttcggccag aacttgtcag tgacaagcat cagcttctga tcaaagacgg
    11761 acgacatcct gtagtagaaa aagtattagg acatcaggaa tatatcccta acagcgtaga
    11821 aatggcagaa gacgaaatga tcttgctgat tacaggaccg aatatgtctg gtaaaagtac
    11881 gtatatgcga cagttggcat taactgttct gatggcgcaa atgggctgtt tcgttccggc
    11941 aaaacaagca gttttaccta tttttgatcg gatttttacc cgaattggtg caagtgacga
    12001 cttaattgct ggacaaagta cttttatggt agagatgatg gaagccaatc aagccctgcg
    12061 ttatgctacg ccaaacagtt tgattttgtt tgacgaacta gggcgaggaa cagcaaccta
    12121 tgacggaatg gcgttggccc aagcaatcat cgaatatatc catcgtcatg tacaagcaaa
    12181 aacactcttt tctactcatt atcatgaact gacggtactt gagaaagaac ttccgcagct
    12241 taaaaatgtc catgttggag ccgttgaaaa agacggagaa gttgtcttct tgcataaaat
    12301 gatggatgga cctgcggata aaagctacgg catacatgtg gcaaagatcg caggattgcc
    12361 ttccaatcta ctagaacgag cagcagatat cctgcatacg ctagaaaatg gcgataacgg
    12421 acaccatact gttccagctg aagtagaaga gaaaactaaa acagccaaac aggagcaaat
    12481 aaaaccacct gcagaaaaag taaatcaaga ccatatacaa gaccgtgtaa aagaagaaaa
    12541 ccaacagctt tctttattca gtgaattatc cgaaaatgaa acagaagtca ttgaatcact
    12601 gaagcaaata aatttactag aaatgacgcc aatggatgca ctaaacacat tatatgaatt
    12661 acaaaaacaa ctgtaggaag aaggcggata tcgatggcca aaatccaaga attatctgaa
    12721 cgtttagcta atcagatcgc tgcgggagaa gtagtggagc gaccagcttc cgttgtgaaa
    12781 gaattagtgg aaaatgcaat tgacgccggc agtactcaga ttgatatctt agttgaagaa
    12841 gcgggactgc ggacgatcca agtgattgat aacggagaag gcattttggc agacgacgta
    12901 gagaatgcct tcaagcggca tgcgacaagt aagatccata atcgcgacga tctttttcgc
    12961 atacgaacgc tgggctttcg aggggaagct ttgccaagta ttgcctctgt ttctgaaatg
    13021 actctggaaa cggcgaatca ggaagaaaaa caaggcacct atctgtcgct gaaaggcgga
    13081 gaagttattg agcatcgacc agcaccactg cgtcaaggaa cgaagattac cgtcagcaat
    13141 ctgtttttca atacgccagc ccgcttgaaa tacgtgaaaa cactgcacac agaattagcg
    13201 aacatcgggg acatcgtcaa tcgactggca ttgagtcatc catcgattgc ttttcgttta
    13261 gtacatgatg gaaacaaaat gctggcaact gctggaaatg gcgatctgaa acaaacaatt
    13321 gcgggaatct acggattatc taccgcaaaa aaaatgctga aaatcgaagg aaaagatcta
    13381 gattttgagt tatttggcta tgtttcattg cctgaggtga ctcgtgccag taggaattac
    13441 ttatcgacga tcatcaatgg acgttttatc aagaactttt ccttgaataa agcaatcgtt
    13501 gcaggttatg ggtcaaaatt aatggtcggt cgtttcccta ttgctgtatt ggaaatcaaa
    13561 atggacccgc ttttagtaga tgtaaatgtt catccgacga agcaggaagt ccggctgtcg
    13621 aaggaaaaag aactaacaga gctaattagt aacgccattc aaaaagcatt gtctcaagaa
    13681 aatctgattc caagtgcagc ggataatcta cggtttaaga aaaaagtacc tgatcagcct
    13741 aaaacagaac aaatcgaaat cgatttgtcc tacgcttttg aagaagaaaa acctaaaaag
    13801 ccaagtagtt tgaattttga tcgagagaca ggtacctttt tcgtagaaga actaaagaac
    13861 acatttgttg acagaattgt tgataagtct gtggaaaatg ttgaaaacta ttctggtttt
    13921 tctgaagaaa acaccactta tccagaaaaa acagcgaaaa cggtggataa gtttggtgaa
    13981 aatgttgata actttgtaga aaaaacagtg gaaaaatctg aaaagaaaga atttcccatt
    14041 gtggataaca tggagaaaac aaatgaagaa caagcagata aatccgtaga agaggatgaa
    14101 aagatttacg aggaagaatt gacactccat cctgaatttg atttcactca aggaaacgac
    14161 aaaacattag aacaagccat caagaaatta gaaacggaaa aaccagtaca acgttttcct
    14221 cagttggaat attttggaca gatgcacgga acgtatcttt ttgcccaaag tagcgatggg
    14281 ttgtacatca ttgatcagca tgctgcccaa gagcgcatta agtatgaata tttccgggaa
    14341 aaaattgggg aagtatctga tgatctgcaa gaattgcttg ttccatttgt gttagactat
    14401 ccaaacagtg atgctttgaa attaaaagag caaaaacact tgctagaaga tgcagggatt
    14461 tatttagaag aatttggtca aaacagcttt attgtacggg cccatcctac ttggtatccc
    14521 aatggagaag aagaatcgat tattcgggag atgatcgata tgctgctgac aactggaagt
    14581 gtgagtgtaa aaaaattccg tgaagcaaca gcaatcatga tgagttgcaa acgatcgatt
    14641 aaagccaacc attatttgaa tgaagcacaa gcacgtgtgc tgctgaagga tctggcacga
    14701 tgcgaaaatc ctttcaattg tccccatggc cgtccagtat tgattcactt taccaattcg
    14761 gatatggaaa ggatgttcaa acgaatccaa gacccgcatt aagtcaaaga gaagagggaa
    14821 aacatgaaga ttgtattagc atcccaatca ccaagaagaa aagagttatt ggctcatatc
    14881 gtttcagaat tcgagattga acctgctgat atcgatgaaa cgccatttcc agacgaacag
    14941 cccgtcgatt atgttcgtag aatggctgaa gaaaaagcac gaactgtctg ggaacaagat
    15001 gaaaaagaag accatttagt gattgccagt gacacgactg ttgttctcaa tcaagagatc
    15061 atgggtaaac cagaagatct ggctgaggct gaattcatgc tgaaaaaact aagcggggaa
    15121 acacatactg tatatacagc tgttgtttta aaaacaaagg aacgagaaga acggatacta
    15181 gcagaagcac atgtcacttt ttatcctttg acagacgaag agatcaaacg ctacttggag
    15241 acgggggatt atgcggataa ggcaggggca tacggcatcc aaaatgctgc gagtgtattc
    15301 gtcaaagaaa tttctggaga ttattacagt atcgtaggat ttccgattgg tgctgtcaat
    15361 caggcactga aaacattcat ttaaacaaaa aagcggacta tctgattttc gattgaaaat
    15421 ccatgatagc ccgttttttt tattgaaaag attacttatc tactctgact aagattttac
    15481 ttcagcttta ccgactgctt cgcccgggcg aacaagtcct gattggtcaa gagaaagtcc
    15541 tgcaactttt tcattattgg tcaaagcaac aataatgtca gttttcttgc cagcttgttc
    15601 tatttctggc aaatccattt cagcaatcag ggtctctggt gtgacaaggt atccttcttt
    15661 cacgaataca ttgaaaggaa caccctttag ttcaacagta tctaatccca tgtgaacaag
    15721 tacttccagc ccctcattag aaagaatacc aatggcatgt tttgtttcaa agacactcgt
    15781 cactttaccg tttactggtg catagacttt gccgtttgac ggttcgacag cataaccgtc
    15841 acccatcatt ttttgtgaga aaactgggtc atttacttca gttatttcct tgatggtccc
    15901 tgcagcgact gcgaataatg tttcttttgt tgcaactgcc gtaccagttg ctgaaacagg
    15961 ttcgaaagac tgtggcgttg ctgttgcgcc atttgctgtt tgaagttgta catcagcagc
    16021 attttcagga acagcaggat caatcttatt caaaattccc tgacgtctaa agatgatcgt
    16081 caagccaaat ggaatgatga cagcaatgat cattgcgatg atgaaaggta cccatgtttc
    16141 tgcttggata gctagtattc ctggcaaacc gcctactccg atcgcgttag ctcgaacacc
    16201 catcaagtta gcaaacatgc cagctaatcc agagccgacc attgcagcga cgaaaggata
    16261 gacgtatttc aaattgattc cgaacatagc aggttcagtt acgcctaagt aacaagaaat
    16321 cattgctgga atcgagattt gttcttcttt tttattccca cgatgtaaga agatgatggc
    16381 taagactgca gagccttggg cgatatttga taaagcaatc atcggccata aattcgttga
    16441 tccgaaatct gcaatcagct gcatatcgat ggcattggtc atatggtgga gaccagtaat
    16501 cactaaagga gcatacagga atccgaatac ggcactaaac aaccagctga tagaagacgt
    16561 caatccagta tttacgatat ttgagatcca gcttccgact gtccagccga taggacctaa
    16621 aataacgtgt gcagctaaaa cagttggtaa caatgaaaac aatggaacaa aaatcatcga
    16681 gatcgactga gggatatatt tgcggaagaa gatttctaga tacgctaaca taaaccctgc
    16741 cagcatggct ggaataactt gcgcttgata accaatcatc tggacttgag caaaaccaaa
    16801 atcccaaaat ggaatatctg ctgcggctgt ggaagctaca ctataagcat ttaataattg
    16861 cggagaaact aacgtaattc ctaaaacgat tcctaatatt tgtgtagtac ccatcttttt
    16921 tgcaatactc caagtgatac ctactggaag aaaatggaaa attgcttcac cgggtaacca
    16981 caaaaaggca ttgacaccat tccaaaactt cgagtgttca acgattgttc caccaagact
    17041 ttcaaactgg ataccttcaa gtacatttct gaatccaagg atcaatccac cgacaatgat
    17101 tgcaggaatc agtggtgtaa agatttctgc taaaacggca atagcacgtt gaacgggatg
    17161 aaggttttgt tttgcagctg cttttccttg ttctttggaa accccttcaa ctccagatac
    17221 tgcaacgaag tcgttataaa aagtagaaac atcgtttcct atgatgactt ggaactgtcc
    17281 tgcattcgtg aacatccctt ttacgctagg tatatcttcg atcgcttttt cgtccgcttt
    17341 tcctggatca ttcaggacaa aacgcatacg agtcgcacaa tgactaactg cagcaatatt
    17401 ttcttttccg ccgatatcat gtaacagctt ctcggcatct gcttgatatt ttgccatatc
    17461 aataaacccc tttctcttaa cttgtctatg caagttattt cttcatttac tatacttttt
    17521 ttcggagaga attgcaagcg ttttattaaa aagtacgaga gaaaatgata tcgttttaaa
    17581 aagcttaaaa taagcttttc ttaaaaatag gaacattgaa atacaaaaag agctgtagta
    17641 tagtgaagaa atgatggaag aacgagtgag ggaaaaaaca tgaataagtt caatgaaatt
    17701 tttttagatt tggaacagaa aattttagcg aaagaatatc ctccgcattc gcttttaccc
    17761 agtgagaacc aattgatcaa aatttatggt gtttcacgag aaacaattag aaaagcgctg
    17821 aatcttttga ctacggaagg ttatattcaa aaaaaacaag gaaaaggttc gatcgtactg
    17881 gatcggaatc gcttcgactt tcctatttct ggattgacta gctataaaga gctacaggag
    17941 acccaacgta ttccaagtga aacgatcatc catacgttag aagaaacaga agtgacaaaa
    18001 gcattgaacg agatcactgg ctgggagatc ggtgcgcctg tttggcattt gatacgcgaa
    18061 agaaaaatca atggcgaagt agttatatta gacaccgatt atctattaaa ggaaatcgtt
    18121 cctattttaa ctccagcaca agcacaagga tcgatctatg aatattttga aaatgagctt
    18181 tctttgacga ttgcttacgc gcaaaaggag atcacagtag aagaagtaac ggaaatcatg
    18241 aaaacagcga tggatcttca cgatgacact tatgcagtag tagtcagaag cctcgttcat
    18301 ctggaagata cccgctgttt cgagtattct gagtcggttc accgattaga taaattccgt
    18361 ttcgttgagt ttgcccgacg ccgaaaagtg taaatagttc aaaagaaagc aaattgttgg
    18421 catctgtcca ctttggcgct atgataggat cacaaagggg gcatttttaa tggataaacc
    18481 aataagacca gaaaaagaag aactgaaaga aaagttatca cccatggctt atgcagtcac
    18541 acaagaaaat gcaacagaac gaccatttag cgggaaatat gatgattttt acgaaaaggg
    18601 gatctatgta gatatcgtca gcggtgaacc gttattcagc tctgcagaaa aatatgatgc
    18661 aggttgtggc tggccatcat tcagcaaacc aataacgaaa agacaagtgc gcgaaaaagc
    18721 cgatttttct catggcatgc atcgaataga agttcgaagc aaagaagcag attctcatct
    18781 cggacatgtg tttaccgatg gaccagtaga tcaaggtggt ctacgttact gcatcaattc
    18841 tgcctcactg aaattcattc catatgaaaa gatggatgaa ttaggttatg gggaattcaa
    18901 atcacttgta gaataatgga aatacacaaa gagcggtaca atacggggat ttactcgatc
    18961 gtccgctctt tttttattca agcgattgct atttgaaaga aacgctccct agactccttt
    19021 tttcacaagg tgaaatgtgc tacaataaag ggaacgtatg tgtgcaaaag aaagaggact
    19081 agaatatgta tgaatatctg acaggtgtgg ttacctttat caacccctat tacatagtag
    19141 tagaaaccaa tggtatcggc taccagatcg cactaggaaa tccttaccgc tacagtagca
    19201 aactaaatca aaaaataaaa atttatgtcc atcaggtaat acgtgaagat gcgcatcttc
    19261 tgtatggatt tgattcgcta gaagaaaagc aactgttttt gcgattagta agtgtttcag
    19321 gaatcggtcc gaaaagtgcc cttgcaatca tggcaagcga agaccactcc ggtttgattc
    19381 aagcagtaga atccagtgat gtcacttatt tgacgaaatt tccaggagta gggaaaaaga
    19441 cagcacaaca aatgatcttg gatctaaaag gaaaattcgg tgagctgagt atcgatacac
    19501 cttttagtct atttgatgaa gcaaacacaa aagacgctac agctctatct gaagcaatgg
    19561 aagctctctc ggctcttggt tacagtgatc gagaaatcaa acgtgtagaa aaacaattaa
    19621 aagaagtaga taatcagaca acggatgagt atctacgcca agcactcaag ctaatgatga
    19681 aaaaataagg aggagaccaa atgacagatg aatacttgtt atcaccagaa tctggcgaaa
    19741 atgaactgag tttggaaaaa tctcttcgac cacagctgct ttctcaatat atcggacaga
    19801 gaaaagtcaa acaagaactt cagatttaca tcgaagctgc cagaaatcgg gaagaacctt
    19861 tagatcatgt tctgttgtac gggcctcctg gattaggaaa aacgacgatg gccatggtca
    19921 ttgctaatga gatgaccgta aatattcgta cgacgagtgg ccctgctatc gaacgtccgg
    19981 gtgacttagt agcaatcttg aatgaactgg aagcagggga tgtcctgttt atcgatgaga
    20041 tccatcgatt acctagagtg gtagaagaga tgctttattc agctatggaa gatttctatg
    20101 tagatatcat ggtaggacaa gggccaaccg cacatccggt tcatttccct cttcctccct
    20161 ttactttgat tggcgcgacg acacgggcag gtatgctttc tgcaccttta cgagatcgat
    20221 ttggcatcat ttctcatatg gaatattatg acgaacagga tctgcaggag atcgttttac
    20281 gttcttcgga tatttttcaa acagagatca ttgaagaagg ggcattggaa attgctcgcc
    20341 gatcacgagg aacgcctaga atcgctaatc gcttgctcaa acgggttcgt gattttgcgc
    20401 aagtccaagg agacgggaaa atcgatcgtc ctatagcgga tcaggcactt actttgctac
    20461 aggtcgatca ggctggattg gattatgttg accagaaatt gttgcgaaca atgatcgagt
    20521 tatatggcgg cggaccagta gggttaagta cgctttcagt aaacatagga gaagagcgtg
    20581 agacggtaga agatatgtat gaaccgtatc tgatccaaaa aggattttta aagcgtacac
    20641 ctagagggcg aattgctact gcttatgcct atgaacattt tggttacgac tatcatatat
    20701 gatttttgct tttagattgg aggaaaatga atgagtgata caacacgtac gaaacaattg
    20761 atcaaaaacg tattgattga tttgtgccaa acgcaatctt ttcgaaaaat cagcgtccaa
    20821 aaaatcgcaa acgaagcaaa aatcaatcga caaacatttt attatcattt tacagataaa
    20881 tacgatttgc ttcgttgggt ctattgtgag gatgcattgc attatttaga tccacaatgt
    20941 ctttcacttg ataattggga agagcaagca ttgaaaatgc tgaaagcaat tgctgagaag
    21001 aaagaatttt atgcaaatac tgtgctttct gacccagaaa ttttacaaaa agagttttct
    21061 caactgatcc aaaaatcatt tatcaagatt tttgaacaaa tggatgaaga aagacaactt
    21121 tctgcaagag acaaactatt ttatgctcgt tttttttctt acggttgcag tggtgtatta
    21181 gtcaattgga tcacgaatga atatgaagaa acaccgatgg aaatcgctat gcagcttttc
    21241 cgtttagcca aggatacgga attatattct tatcgtcttt atgcaaaaga agagttggaa
    21301 gaacagtaat agaaaaattt tttgttggaa aagcaataat cgctaaaagg agatggggaa
    21361 atgacaaaaa ttttatttgt ttgtttggga aatatttgcc gttcaccgat ggcagaaggc
    21421 ttgctgagaa aacgaatagc attagaagga agggaagcag aattttttgt ggattctgct
    21481 gctacaagca cgtatgaagt tggaaagaca ccgcaccctg gaacgaaaaa aatattaaac
    21541 caagaaaaag tagatatgac gaatatggtt gctcggcaaa tcacacccca cgattttgaa
    21601 acttttgatt ggatcattgg catggatcaa gaaaatgtag aagaactgaa acgaagagca
    21661 ccacgctcag cgcaggggaa aatccatttg tttttaagta gcattcctgg aaaagaaaaa
    21721 gaaaatgttc ccgacccata ctataccaat aactttgatg agacatatca cttgatcaca
    21781 gaaggcctga atcattggtg gaatatttgg attgatgaaa ataaaataac aaacaatttg
    21841 taaagttaac gctttctttt taaggcgatg ttagagcatt tccttgtttt tttgtaaaat
    21901 gttaggtaaa ataaaattaa ttaagtaaca ctttcttagc taaaggagga gtgcattcga
    21961 tgaacgaaga gacagtatcg ccaatgaaga agcttacgaa gttagttact catacttcgt
    22021 atcaactttt tgattctttt gtaaatgaca ctgaagaaca tgctaagaat gtacgattgc
    22081 aaggaaagtt tattcttgag ctgcaaaaag ctgctactga acatagtcta gttatcttac
    22141 aagtaagagc aaatcgtttg agtagcaagt atgaaacgat ctcaggctgg gtggcgacaa
    22201 aaacggtcga aaaagaccga gtgatgatcc gcctacaaaa cgaagagcag cagatccgga
    22261 tggttcctgt agaacaaatt gaaaaaataa caatcctttc accagcaggg gaacaggaac
    22321 ggttatcaaa ataatttata ccagatagaa aagcacgtat gagcttgtac catcaatcat
    22381 tactaatcaa aaataaccga atgctttaag ctgatatcag taaaacggaa cgttttagga
    22441 tgagtatcac agcatgacag cattgggttg attagttgaa tcgattggat ggtacaagtt
    22501 tttttgataa aaaaaggtcc tgctagccgg ggagctagca ggataaggag ttaaaaatga
    22561 aaaagtgttg ttagggttgt ttgttggtat gcttatataa tagtcactag ttgtgaatat
    22621 tttgtgacga aagttttcga aagttgttaa gtt
//
LOCUS       DAZKNH010000041        22559 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000041 DAZKNH010000000
VERSION     DAZKNH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.041"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1139
                     /locus_tag="ACG8MG_001462"
     CDS             <1..1139
                     /locus_tag="ACG8MG_001462"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFQ0375513.1"
                     /translation="LVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRINK
                     VPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPKQ
                     KSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDGL
                     DGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLGD
                     TGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTAD
                     KKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAILL
                     FELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1132..1932
                     /locus_tag="ACG8MG_001463"
     CDS             1132..1932
                     /locus_tag="ACG8MG_001463"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0375514.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1932..2759
                     /locus_tag="ACG8MG_001464"
     CDS             1932..2759
                     /locus_tag="ACG8MG_001464"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0375515.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2764..3471
                     /locus_tag="ACG8MG_001465"
     CDS             2764..3471
                     /locus_tag="ACG8MG_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0375516.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            3571..4437
                     /gene="rfbA"
                     /locus_tag="ACG8MG_001466"
     CDS             3571..4437
                     /gene="rfbA"
                     /locus_tag="ACG8MG_001466"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFQ0375517.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4451..5023
                     /gene="rfbC"
                     /locus_tag="ACG8MG_001467"
     CDS             4451..5023
                     /gene="rfbC"
                     /locus_tag="ACG8MG_001467"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFQ0375518.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5045..6073
                     /gene="rfbB"
                     /locus_tag="ACG8MG_001468"
     CDS             5045..6073
                     /gene="rfbB"
                     /locus_tag="ACG8MG_001468"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFQ0375519.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6171..7022
                     /gene="rfbD"
                     /locus_tag="ACG8MG_001469"
     CDS             6171..7022
                     /gene="rfbD"
                     /locus_tag="ACG8MG_001469"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFQ0375520.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7056..9089
                     /locus_tag="ACG8MG_001470"
     CDS             7056..9089
                     /locus_tag="ACG8MG_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375521.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9133..10413
                     /locus_tag="ACG8MG_001471"
     CDS             9133..10413
                     /locus_tag="ACG8MG_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFQ0375522.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            10623..11429
                     /locus_tag="ACG8MG_001472"
     CDS             10623..11429
                     /locus_tag="ACG8MG_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0375523.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11441..12661
                     /locus_tag="ACG8MG_001473"
     CDS             11441..12661
                     /locus_tag="ACG8MG_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375524.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12651..14237
                     /locus_tag="ACG8MG_001474"
     CDS             12651..14237
                     /locus_tag="ACG8MG_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0375525.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14276..16414
                     /locus_tag="ACG8MG_001475"
     CDS             14276..16414
                     /locus_tag="ACG8MG_001475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0375526.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(16782..17765)
                     /locus_tag="ACG8MG_001476"
     CDS             complement(16782..17765)
                     /locus_tag="ACG8MG_001476"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0375527.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            18143..19534
                     /locus_tag="ACG8MG_001477"
     CDS             18143..19534
                     /locus_tag="ACG8MG_001477"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0375528.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            19550..20590
                     /locus_tag="ACG8MG_001478"
     CDS             19550..20590
                     /locus_tag="ACG8MG_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0375529.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            20609..21694
                     /locus_tag="ACG8MG_001479"
     CDS             20609..21694
                     /locus_tag="ACG8MG_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFQ0375530.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            21727..>22559
                     /locus_tag="ACG8MG_001480"
     CDS             21727..>22559
                     /locus_tag="ACG8MG_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0375531.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     F"
BASE COUNT         7353 a         3647 c         4576 g         6983 t
ORIGIN      
        1 tgcttgtttc ttttagtatg gttataagat tttttctgac aattttatta tcactgatat
       61 taacacctgt attcaaaatc atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa
      121 gaagaataaa taaagttcca atgccttcag cgggcggact gcctatcttt atttcatttg
      181 ttatatcgac tttattctta ttccgtaaaa ttatccctca atcgtatatt gtaccgatct
      241 tgattgcttc tttagtaatc atagtcacag gagtgctcga tgataaatat gagctgacac
      301 ctaaacaaaa aagtataggg atcttgattg cttctcttat tatttatttt gtagcagata
      361 tacgtattga ttccttcacg cttcctttta ttggatatat ccaactaggc tggcttagtt
      421 ttcccgtaac gattttttgg atcttcggta tcacgaatgc ggtcaatctg attgacggtt
      481 tggatgggct ggcggcaggg atttcattga tcggtttaat tacgatagga atcattggat
      541 attttttcct ccatgcttct acagtgtata ttccagttgt tattttctgt cttgtagcaa
      601 gtattatcgg tttttttcct tacaattttt atccggcaaa gatttattta ggcgatacag
      661 gagctctatt cctagggttt atgatggccg tcctttcact acaagggctg aagaacgtga
      721 cttttgtttc atcgatttct ttgctgatcg tcatgggagt gccggtaaca gacacgtttt
      781 ttgctatcat taggagaaaa gcaaaccgtg tttctttttc tacagcagat aaaaaacatc
      841 ttcatcaccg actaatcagt ttaggcttta ctcataaagg tgctgtcctg acgatttatg
      901 cgatggcttt gatgttttct ttcacagcta tggtcatgaa ttatactgga agaatcggaa
      961 cgatcatatt gattattgcc atgttgtttg ctgcaatttt attatttgaa ttgattggtc
     1021 tgatcaatga gaaacatcag tttattttaa aaacattgcg atttttaggg aataaagagt
     1081 accgtacgca agtaaaacag aaatatttga agaaatggaa ccgtcatata gatgaataac
     1141 aaattaaacc agccgatcct tattagtatt gtgacggcaa acagtgataa aatttttcgg
     1201 acactagata attttatttc aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt
     1261 gacaacaatt cacttcccga gtacaaaaaa agactaacag agtataggaa atatccctac
     1321 atcgatatct attttcatga tgaaaattcc ggatttggtt atggacataa tcacaatctg
     1381 atagctagcg attctcgcta tggcattatt tgtaacccag atattttagt ggatgaagag
     1441 acagttacag cattggtctt gttattgaaa gaacatccgg aatgtgcgat gttagcacct
     1501 aagattctta atgaagatgg aacaactcag cacctcatcc gtgagcagct ggctgttttt
     1561 gattacgttt tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg
     1621 gctgtgtttg aatgccgagg attgcctact gatgctaata gttatgtacg gatgatctca
     1681 ggtagtttta tggtagctga tattgataaa tttaaagaga taaaggggtt tgatgagcgt
     1741 ttcttcatgt actttgaaga caacgacttg tgtttgagtt ttgaaaaagc aggttacaag
     1801 ttattgtaca caccctttta tgcagttatc catttttacg ggaagggtgc gcatcgaaat
     1861 gcaaaactgt ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa gtgggggtgg
     1921 cgttttttct gatgaaacca gaaatattag tagtcgttgt gctttatcag caactattca
     1981 gtcaatctcc agcttatgga ccattaagca aagcacttag cgaaaagcaa attcagctta
     2041 ttatttacga taatagtcct caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg
     2101 tttatcatca tgatccgcaa aatcctggtt tagcggtggc ttataatttt gcgttgtctc
     2161 aagcagatga aggaacacgt tgtctcgtca ctctggatca ggatacgagg ctagtcgacg
     2221 attattttga actattgcgt aaagtaacct ttacagatga atgtgttgct gcagttccga
     2281 tgattttttc tgggagccga caagtttctc ccctatattc agatcgttat atcaatcgta
     2341 cagctcaagc agtagaagtt gaaggcccaa cagctgaacg aatcatggca atcaattcag
     2401 gtgttgcctg gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct gctttttctt
     2461 tagatttttt ggatcattgg ctgttttgga aagtggatca gttggaaaaa actgtggaga
     2521 tccttcctgt acgaatggaa catgatcttt cggttttaga ctatgaaaaa gtttcaagta
     2581 atcgataccg ttctatttta caagcagaaa atcgatttta tcatgaatat gatcaaaggc
     2641 atttatccag tcaccgacga caattattgt taagaacagc taaacagttc ttgaccgtca
     2701 aaaaccggca tatctggcgg atgactttgc gctcatatgt taagaattgg aaggtgtgaa
     2761 ttcgtgcgtt cggtttgtat agctaccttt aatggaggca gatatttaag agagcagtta
     2821 gatagtattt tatctcagct ttcagcagaa gatgaagtag tcatttctga tgatgggtca
     2881 gtagatgata catgggatat attaactgaa tacgcagaaa aagataagcg aatccagctt
     2941 ttttcggggc ctcgtcaagg attgatcgct aattttggtt atgcgatcca gcagacgaaa
     3001 ggagatctaa tctttttggc agaccaagat gatgtttggt tggagacaaa agtgaaaaag
     3061 atcactgact attttgagca gaatccggat cagctagtag tagtttctga tttaatcatt
     3121 gtggacagcg agctgcaaga gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa
     3181 ggatggctta ataatgtgat aagaagtcac tatattggtg ctggtatggc tttccggtct
     3241 tctttaaaac ccgatatttt gccgatcccg tcagaagtgc cgatgcatga tatgtggatt
     3301 ggtttgctgg gaggaaagaa gactgggttc ctaagagaac cgttaactct gtaccgaaga
     3361 catgatttaa acgcgagtga gattgaaaca cattctaaat tacggcaaaa aatttcttgg
     3421 cgtgttcatc tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg
     3481 tttaaatcct taatatttcc atgacaaata taacaattga tgagataata acaaagtgaa
     3541 atttgaaatc aaaaaggaga gcagatatcc atgaagggaa tcattcttgc tgggggcagc
     3601 ggaacacgtc tgtacccttt aacaaaggca acatcgaaac aattgatgcc gatttatgac
     3661 aaaccaatga tttattatcc aatgtctact ctgatgttgg cgggaatcaa agaaattctg
     3721 attatttcaa cgccacaaga tacaccacgt ttcaaggaat tatttggaaa cggaaatgat
     3781 ttaggactgc agatcgaata tgcagtacaa gaaagtccag atggattggc acaagcgttt
     3841 atcattggag aagaatttat tggagatgat agtgtctgct tggtattagg ggacaatatt
     3901 tactatggtg gcggtctgtc taaaatgttg caacgagcag cttcaaaaga tacaggcgcg
     3961 acagtatttg gctatcatgt caatgatcca gaacgctttg gtgtagtaga atttgatgag
     4021 aatatgcaag ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta
     4081 actggccttt atttttatga caatgaggta atttctatcg ctaaaggtat caaaccttca
     4141 gaacgtggag aactagagat tacggatgtc aataaagcat atttggataa aggaaaacta
     4201 tccgtagaac ttatgggacg cggatttgct tggcttgata caggaacaca tgaatcattg
     4261 cttgaagcat ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt
     4321 ttggaagaaa ttgcttaccg tatgggctac atcgataagg aacaattgct ggcccttgca
     4381 cagccattga aaaaaaatca atacggtcaa tatttattga atttggccgc tgaataagga
     4441 gtccttacag atgaaagtaa cagatacaaa attaacagat gtcaaaatcg tagaaatgga
     4501 cgtctttggc gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa aatttgcaga
     4561 acatggattg aaatatgact ttgttcaaga caaccattca ctatcagctg aagctggtgt
     4621 ccttcggggg cttcatttcc aaaaaggaga agctgcacag acaaaattga ttcgggtagt
     4681 cactggagca gttttagatg tagtagtgga tatccgcaaa ggtagcccga cttatggtca
     4741 atgggacgga tttatcttat ctgaacataa tcatcgacag ttgcttgtac ctaaaggata
     4801 tgcccatgga ttcgttactt tgacaccaaa tgtgaacttc ctttataagt gtgataatta
     4861 ctataatgca gaagcagatg ggggcatcgc atttgatgat ccagatttag cgattgattg
     4921 gccaattgat ccatcaagag cgattatgtc ggaaaaagat caccgtcatc cgacattaaa
     4981 agaatttgaa gcagaaaatc catttgttta cggagaaatc taagaattaa ggagaaattt
     5041 tcttatgaaa aacatcattg ttaccggagg tgccggattc atcggctcta atttcgttca
     5101 ttacgtggta aacaatcatc cagaagtgca tgtcactgtt ttggataaac tgacttatgc
     5161 aggaaacaaa gagaacttgg ctggacttcc tagcgatcgc gtagaattgg tcgtaggaga
     5221 tgttgcagat gcagaattag tggaccgtct agtaaaagaa gcggacgcag ttgttcatta
     5281 cgcggcagaa tcccataacg ataattcatt aaaagatcct ttcccatttg tacaaacgaa
     5341 tatcattggt acgtatacac ttttagaagc ttgccgcaag tatgatgtac gctatcatca
     5401 cgtatctact gacgaggttt acggcgatct tccattacgt gaagacctgc caggacatgg
     5461 ggaaggcgaa ggagaaaaat tcactgcgga aacaccatat aatccttcaa gtccatattc
     5521 ttctacaaaa gctggttcag atctgttagt aaaagcatgg gtgcgttctt ttggcttacg
     5581 tgcaaccatt tcaaactgtt caaacaacta tggtccttat cagcatattg aaaaatttat
     5641 tcctcgccaa gtaacgaata tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa
     5701 gaatgttcgt gactggatcc atacgaatga tcattcttca gcagtgtggc tgattttgac
     5761 gaaaggcaga attggcgaaa cctatctgat cggtgcagac ggtgaagaag ataataagac
     5821 cgtgatggaa ttgatcttag aaatgatggg tcaaccaaaa gatgcgtacg accatgtaaa
     5881 cgaccgtgca ggacacgact tgcgttatgc aatcgattcg acaaaattac gggaagagtt
     5941 aggatggaag cctgagttta cgaacttccg tgatgggttg gctgacacga tcaaatggta
     6001 cgaagaacat gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa
     6061 aaatggacaa taatacagag aaaatgtcac tgtattaatg gaaaaacaca tgtgaggcag
     6121 gcgctcgcct gtctcttttt taaaataaaa atcaaggagc ggataatcat atgtttttat
     6181 taactggagg aaatgggcaa ctaggtacag aattgcgcca tttattagat gaaaaaaatc
     6241 tgcaatatgt ttcaacagat gcaaaagact tggatatcac agatgccgag aaaacaatgg
     6301 cttacatcac agaattaaaa cctgatgtta ttttccattg tgcagcttac acagcagtag
     6361 ataaagcaga agaagaagca aaagaattag atgagaagat caatgttgat ggtacaagaa
     6421 atgtagcact tgcagcaaaa gctgtgaatg ctcaattcgt ctatatcagt acagactatg
     6481 ttttcgatgg aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg aatccactaa
     6541 atgaatatgg tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt ttagaagact
     6601 attatatcat acgaacttct tgggttttcg ggatctatgg acacaatttc gtcttcacga
     6661 tgcaacgcct agcggaaacc cgcgacaaac tgactgtagt aaatgaccag tttgggcgac
     6721 caacttggac acgtactttg gcagaattta tggtatatgt catcgaagaa aaagcaccat
     6781 ttggtattta tcatttgtcg aacgaaaata gctgttcttg gtatgaattc gcaaaagaaa
     6841 tattgaaaga tacagatgta gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag
     6901 caacacgtcc gcagtattca gtcatgagcc tgaaaaagac agaagagtta ggatttgtca
     6961 taccaacttg gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt
     7021 aatttccttt tatcaaatag aatttggagc atgatatgga aaaacaaaca agaagtgcac
     7081 gacgaaaaga aaagccaaaa gcgaaccagg cagattcgaa tttggtgctt gacctgtttc
     7141 taaaactccg ctatcttatt ggcgttttag tcatcgtggt catcgttact tttaatttaa
     7201 acggaagttc acttggtgtt tgggataaat atgtgtcgca aagagacgat gggaaaaaaa
     7261 ctgatgtgat ttttggacag aatcgggaag tgcggtcaga cgaatggctg gtgcagacac
     7321 ctttttacct ctcacaagct gaagaggact ttccgttggt gaacgaggat tattcattaa
     7381 acggacaaaa catgatcatt gcgtataatt ctcctgtaaa ggatattacg gttatcggaa
     7441 aaccattcaa ctggggattc tttttcctcg gaagagatcg aggactttcc ttttattggg
     7501 cgatgaaatt agtcgtcatg gtcttgcttg gattcgaagt actgatgata ttaacgaaga
     7561 gaaaaaaagc tctatcgcta atcggtgcat ttgcgctgac attttctcca gcagtccagt
     7621 ggtggttcat gcaacatgtg ggcgatttga ttttctttac tttgggactg atgactgctt
     7681 tttatcatta tttctacaat catgataaaa aatggctacg aacgttgatg atgcttttgg
     7741 tggttatttt cggtattggg tttattttag tgatttaccc agcccatcaa gtgatgctgg
     7801 cgtacttgct gattttttat tttgttggcc tgcttattta ctatagcaag aaagtttcat
     7861 gggatctgtt ggatgtagtg ttgattggag gggcagtttt atttattggc ggagtcatgg
     7921 ttcatttctg gctaacgtca aaagacgcat tgttagcttc tttgaatacc ttatatccag
     7981 gaaaacgggt aagtactgga ggaaagtgga cgattggcga atttttttct ttccttacaa
     8041 attggaaaat tccttttgaa gatatcaact attcgaataa ctctgaagtg gccctgttct
     8101 atcacttttt tccaactgta ttcctagcaa gtccatttgt gttatttggc aaaaaagata
     8161 gcgaacaaaa gttactggga cgtattttga tgattttttg tctgttttct atcttttgga
     8221 ttaccgttgg gttgccaaaa tggctagcag aaatcactct gctgtcttat gttcctccat
     8281 taagagccat ccttactttt agttttgcgg cttgtttatt atcgatctgg tttatcgctt
     8341 atatttggag agaagatgtt attcctattt ggtataaatt cattttgttg gcagtaaatg
     8401 catggtctta tttctatgcg attacgcatt cgaaaatgga taattatttt tcggattttg
     8461 aaaccatcgc agttgctgtg ttgggaacag tgattttagc ttttgcatta tttggctgga
     8521 gacgcttgtt ttacctcgct tttgccgcct tagttatcgt atctggcttt ttcgtcaatc
     8581 cagtcgtaga aggtacagga gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa
     8641 tcgatcaaca agatcctggt aaagtttggt tgtctgaaga tgaattatat aacttcaccc
     8701 caacattggg agtgaaatcc ttcaatacag tacgctttta tccagatata gctgcatgga
     8761 agaaaatcga tccgaaagaa gaatacgaga agtattataa tcggtatgct catacaagag
     8821 cttttatcgc taaggaagat acaaagttca ttttagatcg tccagatatg ttttcggtca
     8881 cgttgaattt tgaagacgca gaaaaattag ggatcgacta tgtgatcagt aaacgaccat
     8941 taactgatta caaccaatta tatgaagcgc agttcacgca gctatatgga ccggataaag
     9001 acggttatat gatttttaaa gtagaatatc cggattatcc agatgtacca caaacacaaa
     9061 ctcaggaaaa tcagctagtc gtacagtaat aggctagctg agaataatcc tgaaatgtta
     9121 gtagaaagga gagtggagat aattcaagca atcgtacaaa aatttgtcaa caaaatgagt
     9181 gtcggtttct tgcttttgtt actgacgcta gtcttttttg tttggacagc agggttaggt
     9241 tttccagcaa ctttgtttat atataacaat agttcattac ttatcgcttt tagtgtggct
     9301 attttgctca ttttaaatgt ttataaagca aaaggcgtcg attttcttta tctaggtgtt
     9361 gcgcttgccc ttttcatttt cttttcttac tttcgggaat taagaaatga gtctttattt
     9421 ggagacttac tgtttccttt gattatttta ttttgtttag taattaaatg gatcgagtgg
     9481 acaaaatggg atcgcggtat ttatctgatt gtcttttcgg tcttcctcgg aatcacgtta
     9541 tatcgtgtat ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat
     9601 tcgctttctg atatttggat caatacgaat ataatcggaa gttctttgat gactcttgct
     9661 ttattgatca gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca
     9721 gtagcagttg ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc
     9781 gcgttgattg tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt
     9841 gcgccattta ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg
     9901 gctgtctctg aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat
     9961 cagacgctag gagaaaaatc tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa
    10021 cgaggaaacc aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga
    10081 ttgataggat ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg
    10141 aaagcagatt tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg
    10201 cagtcattta tggaggatac attgacttca tacccatggg ttccgattat ttatatcctg
    10261 ttggctatgg ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg
    10321 gaagaggcat atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc
    10381 gctcattctt ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg
    10441 acaaccgtcg cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa
    10501 acgttagaga aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta
    10561 ccaaaaatat ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac
    10621 aaatgtttaa agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc
    10681 tacagttttc gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat
    10741 gggcgtttat caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac
    10801 tgaaagcaca aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga
    10861 ttccctggtt cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata
    10921 cttatttagt aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat
    10981 ccaatctgta cacccactta ttcttcattc tgattggcat agctgtcgcg cttataaatg
    11041 gtatatatcc ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt
    11101 tcttgacagg attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc
    11161 aattaattac gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta
    11221 atcagtttga ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc
    11281 aaggttatcg tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt
    11341 tgtatttctg ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat
    11401 tgaaaccaca tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag
    11461 actaaaagat atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga
    11521 agccctcaat ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa
    11581 tgtagaaata aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc
    11641 tacaatgttg aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga
    11701 aggaaaaatc tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta
    11761 tgaaaatatc tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat
    11821 ggtcgatgat atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac
    11881 ttattcaagc gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga
    11941 tattttgatt gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat
    12001 ggaaaaattt acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat
    12061 cagttcgatt cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga
    12121 atatggcgat acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt
    12181 taaaacagtg gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga
    12241 cgtgacgaaa gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg
    12301 agaaaaacta tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt
    12361 tttaggaatt gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact
    12421 agatggtatc aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa
    12481 gttaggtttg ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac
    12541 ggttcctttg gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg
    12601 agaaggcatc gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg
    12661 attttgaaat ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac
    12721 caggaagtaa agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg
    12781 gagcaaaaca atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca
    12841 gcgaatttgc tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt
    12901 atgctggaca gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc
    12961 ctggaaaaac attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta
    13021 cattccctaa cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt
    13081 gggcttctta tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc
    13141 aaaaagtttt tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg
    13201 taggtgatac agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt
    13261 tcaaaatgcg accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag
    13321 tagcacaaag ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa
    13381 cccagcaaac gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg
    13441 aaaatgagac actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg
    13501 cgcaacaacc tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta
    13561 tcgattcgaa tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat
    13621 taccagagtt cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc
    13681 gctttatcgg ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg
    13741 ctgaagtcac aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc
    13801 ttcttgagga aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca
    13861 cattgttgga aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa
    13921 cttcgaaaaa aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg
    13981 cagaaacaaa aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct
    14041 tgtcctataa gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag
    14101 aagaagtcaa tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa
    14161 tcctttgcga gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat
    14221 ggatcattga aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca
    14281 aaaaaaagaa attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt
    14341 gcttattgtg ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga
    14401 aaaagaaaat gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata
    14461 taatcttgat gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg
    14521 aaaagccatc ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa
    14581 cggtaaatac ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact
    14641 gaaaaaaggg ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa
    14701 attggaattg aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc
    14761 agatttgaaa gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat
    14821 acttatgcct gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact
    14881 ggatcaaacg tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc
    14941 tatccgaaag tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg
    15001 aaaagaaaat ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga
    15061 atttatagca ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc
    15121 aaaagtgcta aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga
    15181 tgcagatggc aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat
    15241 gggaaataat tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg
    15301 cggtttccgt aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga
    15361 acaaatccct gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat
    15421 cccaggatca actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa
    15481 agcattgaca gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat
    15541 ttctggattt tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat
    15601 tccaacgaag aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac
    15661 gtcttattca aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca
    15721 agaattattt gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga
    15781 cattccattc aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata
    15841 ctttttattc ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt
    15901 ttcttatgca cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga
    15961 tacaacgcag catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa
    16021 taactatgat cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt
    16081 agcagtcact gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt
    16141 tgatgaaaca ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact
    16201 gggtcgttac aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag
    16261 aggttatgaa gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca
    16321 agataaatgg ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga
    16381 aggaatcggc gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt
    16441 gattcgctcg cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg
    16501 actcaaaaaa ataagtcgag gttgggacag aagtgtttaa ctccgagaaa taagaagaaa
    16561 ttcacgaaaa ttgcttttca aatttttgtg aatttcggct tatttaagaa gaagttgctt
    16621 ctgctcccac cgtttattcg tttttaaaga atgagacaaa agtgtttctt acttttgtcc
    16681 cacgctctta ttcatgctca gagcgccaat agatacaaaa tcatagccaa agtttcacga
    16741 accctagctg tcaaagaatc gatattttat tttaatatag gctattttaa aataaatggt
    16801 ttaaggtatt cctctacaaa agccactcta tcaattatca tgggtttaaa ataagaagtg
    16861 atggagataa ccagattgtt cttagcgtca atataaagaa catttccact attaccaatt
    16921 gctgaatata tttcgttctc agaatctaat acccaccata aatagccata tctcatattt
    16981 tgtaagtctt ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt
    17041 acgatttgtt ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga
    17101 tcacttgccg ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac
    17161 cagacattgg ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca
    17221 gcataataat tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa
    17281 tcaacagttg ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca
    17341 gtttgatagt tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt
    17401 tttgtccaat cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt
    17461 gctttcattg ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc
    17521 actttatact ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc
    17581 tctaaagcaa tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa
    17641 gtatcgtctt ttttataacc attccattca tctgtataaa cttctacccc atccttatat
    17701 gcgacaattt gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta
    17761 ttcataagat attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa
    17821 ttttttcgat tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga
    17881 tggattctct gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac
    17941 ttcgacttct ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt
    18001 agagtccttg tcttgattag aggtgtataa agctttttat tagacatttt taaattccat
    18061 tacaatttgc ttataaacta ggtttttaaa ggtgactatg gtaagataat agagatatta
    18121 ttttgtgatt aggagtgtat atatgaataa aaatggagaa tggagtgctg cacgacggat
    18181 attcattata ttaatggatg ttattgtata caatgcagca atatatagta gtttcttact
    18241 gaagtttcgt ggagagatcc cattacgaaa tttagaaact ttccaacact cagcaattat
    18301 tatttcagtg atttttattg cgttgaacat tttgttgggt gcatatgttt tttataatcg
    18361 aatgatcagt gacatcgtgt ttgtcacagt catcggtcag gtattgatga cactagggat
    18421 catggtagtg acttttgcgg gccgctggtt tgcttttcca cgtcttgtta tcttactttc
    18481 atttgttctc ggtacgcttt tcttaagtat ctatcgtatt ctcgtatata aactttattt
    18541 acgcgtaagt cgagataaaa aggtcgtcat tattgggtta gaaaaggatg tcgcaccagc
    18601 aatccaaaat tttggttcaa agaaaaacaa caaacataaa gtcgaagcag tcattcttga
    18661 taactattat gcaaacatca aaaatatgat taaagacatc gatattgtct atcttgcttc
    18721 tcatatcgaa gaaactgaaa aaatcaaaat ctatcaatta gttactaaaa atgaaaaaaa
    18781 attgtttttg aatacaacat ttgaaaactt gacattgatc aatccaaata ttatgaattt
    18841 cgaagatgaa agtattattg aagcatctgg atttagaatc caacctgaat atgaactatt
    18901 caaacgtttg tttgatatca tcgtttcact tgttttatta gttattgctt caccatttat
    18961 gttattcaca gcaattttag tcaaagtgac atcaccggga ccaatcatct ataaacaagt
    19021 gcggatcaca aaaaatcaaa aggaattttc aatctataaa ttccgctcga tgaccgccac
    19081 agccgaagca aaatctggac cagttttagc taagagcaat gatgcacgag taacgcctgt
    19141 aggtaaattt attcgagctg tccgctttga tgaattacca caaatcttca atgtgctgaa
    19201 aggcgatatg tcgatcgtag gaccacgtcc tgaacgtcct ttcttcgtcg atcagttcaa
    19261 tgcagaaaac ccttattact acttacggca taatgttcgt gctggtatca ctgggtatgc
    19321 acaagtctat ggaaaatatg tctctgatta caacagtaaa ttacgttttg atttacttta
    19381 tatcaaaaaa tattcattga tgcttgattt aaagatcttg ctgcaaacca tcaagatcct
    19441 atttgataaa atgtcctctc aaggacttga agaagacgaa aatgtgatct cattggcaga
    19501 aattgctttt ccgaatgaaa aaatctttcg ctagttggga ggcgtaaaaa tgaacaatcc
    19561 gaaattcagc ataatcgtac cagtatacaa tgcagaagca actattggtc gaacgattga
    19621 tattctaaaa gaattggatt atgaagagta cgaagtcgtt ttagtaaatg atggatcaac
    19681 agatcaaaca caggaaatca ttcaagaact gattgaaaat gatgaacgtt ttgaattgat
    19741 tactacgacc aatcaagggc ccggattagc aagaaacgaa ggaataagac agtcaaaagg
    19801 agaatatctc ttattcttcg atgcagatga cacgccagta aaaacgatct tgtcagatta
    19861 tgatcatttg ctagaaaaga gtccaaaagc ggatcttgtt gttagttcgt ttgtttttcg
    19921 tacaatggac gagggaagaa tcgtttccga aaaagaaaac ttagttacag aatgtcacta
    19981 tctaactcac gaagagttta taaaagacat gtatcagtta atgaatgaac agttgatgta
    20041 tgttgtatgg aacaaatgct atcgtagcga tattctgaaa aataaccaaa ttctatttaa
    20101 aacttataat agttgtgagg atcgaatatt taacttacat tattatgaat actgtcatca
    20161 agtgatcatg aacccaaaag tagaatatat ttatgaattt gaaggtggga aggggatcac
    20221 taatcaatac cgacctaaca agttcgaaac atttaaagaa ttctattcat tagcaaatac
    20281 tgtaacagac gatattaata aagacggtat ggcggcgctt cttttaaaag ggacaacttc
    20341 agttattttt tctatttata gtacctctgc aagaacttca aaagagaaga aagacgaagc
    20401 aaagcagata ttgagtgatc cagtgatccg agaagccaag caaattgcta agacggattc
    20461 ttcagcaaaa aaaataacaa agttgcttta caatatgcca gctccactat ttttatctgc
    20521 tgtaaaagtt ggaagcttcg ttgaagtgaa aatgccaggg ttaatggctg ctttgaagcg
    20581 agtatattag ttaatggagg aaaatataat gaaaagaatt ttgcattttc agggacgaat
    20641 gggtttaggt ggagcagagt ctttcatgat gaatttatat cgtaaaattg atcgtacaaa
    20701 ctatcaattt gattttttga tttatgaaga ttatgcagat gttcaagact atcattcaga
    20761 gattgaaaga ttaggaggcc gtatttttgt tgtacctaat cctaagaaaa atatttttaa
    20821 atatttaata gaagtgaata agttactaaa aaaagaatct ttttctatag ttcacaatca
    20881 ggtttatttt ggcgggggaa ttaatttatg gttagccaaa aagaatggaa tcagacagag
    20941 aattgctcat agccatgcaa ctgaagatgg aaaatctcaa aatattgtaa tgaatgtatt
    21001 gcgtaaattt ttaaccaaat tgttgttaca aaatgcaaca gactaccttg ccgttagtca
    21061 agaagctggt gaaagtctgt ttcaaaacca cccctttgag atagttcata atggtattga
    21121 tttagagctt tatagtaaaa attcagaagc taaggttaat aagagaaaag aattggatat
    21181 ctcaatgagt acttttgttg tgggaaacat tggacgatta gagaaacaaa aaaatcaatc
    21241 ttatttgtta gagatttttt tagaaatact aaaaatgaat tctgattctt acttgatttt
    21301 agtaggtgaa gggtcactta gaaataaact gaaagccaaa gcagtagaat tggatataga
    21361 gaaaagagta aaatttttag gagaaagaaa cgatattccc gagttattat ctgtgatgga
    21421 tgtttttgta atgccttctc tttatgaagg tctgccaatg gttggattag aagcacaagc
    21481 agcaaaatta aaattagtct tagcagatac tatttccaaa gatacaaagt taacaaataa
    21541 tgtggatttt gtatcattag atgaaacacc acaagaatgg gcaaaaaaag taataaaaga
    21601 gccgtatgct aataaaataa cacaagaact aaagagttat gatgtttcgt acactactga
    21661 acaaatggag agtatatata gtagagatca ttaaaaatgt tcaagatatt ttaggagtga
    21721 ttatttttga aaatatgtta tttgatattg gcatacaatg atattggcaa cttaaagaga
    21781 ttggtcaatc gattgaacga aaatgcagtt tttattattc atatagataa aaaaactgat
    21841 atagaaccat ttattgaagc atttaaatca tatgataatg ttgaattttt atgtaataaa
    21901 aaccgtgtta aaatttattg gggtggtttt tccattgtac aagcagaaat caatttagta
    21961 aagagagcat tacaaaatga gaaatattta aaatatgtat tattatctgg tgctgattat
    22021 ccaatcaaag ataatgagta tatttataat tattttaaaa agaatagttc agtggagttt
    22081 attcgtggaa tagatctaga tcaaattaaa cataaagaat tttattataa acatatagat
    22141 gtatatcaaa aacatgatta tcctagaatt aataggaata acacaactgc atttaaaatt
    22201 tttagagcta taattaatcg ttgtctgagg atgattaaac ttcccccaaa aattagacac
    22261 cataaatttg atttgtatca tggttctcaa tggtgggctt tgtcgaaaga atgcctaaca
    22321 gaattaatac aaatgtatga acaaaatcaa caagactacc tgaattttaa aataggtatg
    22381 tttgccccag atgaaaaatt ttttcataca ctttttttta attcctcgtt caaaaataaa
    22441 aatgttattg gaggaccaga tttaccactt gaattgaaaa atattgaaga aactacttta
    22501 caaacttcga aattagcaaa tattcatatt attgatcctt ccatgaacaa atggtttga
//
LOCUS       DAZKNH010000042        22282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000042 DAZKNH010000000
VERSION     DAZKNH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.042"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(169..549)
                     /gene="yajC"
                     /locus_tag="ACG8MG_001481"
     CDS             complement(169..549)
                     /gene="yajC"
                     /locus_tag="ACG8MG_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFQ0375532.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(646..1791)
                     /gene="tgt"
                     /locus_tag="ACG8MG_001482"
     CDS             complement(646..1791)
                     /gene="tgt"
                     /locus_tag="ACG8MG_001482"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFQ0375533.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            2009..2707
                     /locus_tag="ACG8MG_001483"
     CDS             2009..2707
                     /locus_tag="ACG8MG_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0375534.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2875..5514)
                     /locus_tag="ACG8MG_001484"
     CDS             complement(2875..5514)
                     /locus_tag="ACG8MG_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0375535.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6133..7164)
                     /gene="queA"
                     /locus_tag="ACG8MG_001485"
     CDS             complement(6133..7164)
                     /gene="queA"
                     /locus_tag="ACG8MG_001485"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFQ0375536.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7357..8531
                     /locus_tag="ACG8MG_001486"
                     /pseudo
     CDS             7357..8531
                     /locus_tag="ACG8MG_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            8535..9176
                     /locus_tag="ACG8MG_001487"
     CDS             8535..9176
                     /locus_tag="ACG8MG_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0375537.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9173..10090
                     /locus_tag="ACG8MG_001488"
                     /pseudo
     CDS             9173..10090
                     /locus_tag="ACG8MG_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
     gene            10087..10758
                     /locus_tag="ACG8MG_001489"
     CDS             10087..10758
                     /locus_tag="ACG8MG_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0375538.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11299..11787)
                     /locus_tag="ACG8MG_001490"
     CDS             complement(11299..11787)
                     /locus_tag="ACG8MG_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFQ0375539.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11988..12254)
                     /locus_tag="ACG8MG_001491"
     CDS             complement(11988..12254)
                     /locus_tag="ACG8MG_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0375540.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12254..12517)
                     /locus_tag="ACG8MG_001492"
     CDS             complement(12254..12517)
                     /locus_tag="ACG8MG_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0375541.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            13038..13178
                     /locus_tag="ACG8MG_001493"
                     /pseudo
     CDS             13038..13178
                     /locus_tag="ACG8MG_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13320..15143)
                     /locus_tag="ACG8MG_001494"
     CDS             complement(13320..15143)
                     /locus_tag="ACG8MG_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0375542.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15741..18446)
                     /locus_tag="ACG8MG_001495"
     CDS             complement(15741..18446)
                     /locus_tag="ACG8MG_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFQ0375543.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18752..19699)
                     /locus_tag="ACG8MG_001496"
     CDS             complement(18752..19699)
                     /locus_tag="ACG8MG_001496"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFQ0375544.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19889..20158
                     /gene="rpsN"
                     /locus_tag="ACG8MG_001497"
     CDS             19889..20158
                     /gene="rpsN"
                     /locus_tag="ACG8MG_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0375545.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20334..20696)
                     /locus_tag="ACG8MG_001498"
     CDS             complement(20334..20696)
                     /locus_tag="ACG8MG_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0375546.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20707..21828)
                     /gene="alr"
                     /locus_tag="ACG8MG_001499"
     CDS             complement(20707..21828)
                     /gene="alr"
                     /locus_tag="ACG8MG_001499"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFQ0375547.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21844..22194)
                     /gene="acpS"
                     /locus_tag="ACG8MG_001500"
     CDS             complement(21844..22194)
                     /gene="acpS"
                     /locus_tag="ACG8MG_001500"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFQ0375548.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
BASE COUNT         6309 a         4763 c         4149 g         7061 t
ORIGIN      
        1 ggtgtttgta tagaaaaata tataaagtta tttattatta tttatatgac acacccgatt
       61 attcaatatt gagcaatata tcccatgctc ttatctcttt cttttttgaa taaaaaatga
      121 ggtaggacat ctttctgccc tacctcgtct ttaaataagc tttgatactt atttttcttc
      181 cttctcttct gtctcttcca gcgtttctgt tgtttcagtt tttacttcaa ctgtctcagc
      241 tggttcttct gttgtttcaa cagtagtcgt tgcatcattt gttacgacag tccctggttt
      301 gaccgttttg attgctgcac gatcgtactc aaggaagatc ccttcgcagt ctaaaacaac
      361 tgtccgtttg tcattgtcga tttctgaaac aactccatgt aatccgccga tcgttacgac
      421 tgaatcacct ggtttcatgc tatctaacaa gttttgacgt tcttgctgtt gtttcttttg
      481 cgaacgtgac ataaaaaacc acattgctaa tagcgcgata aacatcacaa gcattggtaa
      541 tgttcccatt tttttcacct cactaattct gtatctacaa aaaacacttt agcagaaaaa
      601 tgctgataac actagttttt tacttaattt ttaaaagatt ttcctttaga agctttttgc
      661 gttttcttta ttgaacccgt attcttcaaa aaaggcttct ctaaattcaa gcaaattatc
      721 atccatgatt gcttgacgta cttgtttcat tagattcaac aagaagtata aattgtggta
      781 agaagtcaaa cgaatcccaa atgtttcatc acatttgatc aaatggcgga tataggcacg
      841 tgtatagttt ttacatgtat aacaatcaca tttctcatca attggacgga aatcatgggc
      901 atatttcgca tttttcacta ctagacggcc ttgagaagtc atacatgtgc catttcgagc
      961 gattcgagta ggcaagacac agtcaaacat gtctactcca cggataacac catcgatcaa
     1021 tgaatcagca gcaccgacac ccatcaagta acgtggtttg ttttcgggga tcaacggtgt
     1081 agtgaattct aatacacgat tcatttctga tttaggttcc ccgacagata acccgccgat
     1141 tgagtaacca gggaaatcca tcccgactag atctttcgcg ctttgacgac gaagatcttc
     1201 aaaccctgct ccttggatga ttccaaataa tccttgacgg tctggattag catgagcttt
     1261 caatcctcgt tctgcccatc gagacgtacg ttctactgat tttttcacgt agtcgtaact
     1321 ttcatcgaat ggtggacatt cgtcaaaact catcatgata tcagagccca atttattttg
     1381 gatgttgatt gctttttctg gtgataaaaa catacgcgaa ccgttcaagt gatttttgaa
     1441 atgcacgcct tcttcttcga tattccgcat atcacttaat gaaaaaactt gaaagccgcc
     1501 tgaatctgtt aaaatcggtt gatcccagtt cataaattta tgcagtccgc cagcttcttc
     1561 tactaagtct tcaccagggc gtagccataa atgatatgta ttacttaaaa taacgccagc
     1621 acccatttct ttcaactctt ctggggacat tgtttttact gtagctaatg ttcctactgg
     1681 catgaacatt ggtgtgggga aagttccgtg tggcgtgatc aattccccta aacgcgcgcc
     1741 agtatgtttt tctttcttga ttaaacgata gcgaatggca ggttgcgtca ttcatttccc
     1801 tactttctat ataaaatatc ttgacaactc ccaccatcat aatcgtttca agtgcttttt
     1861 gcaagttccc tcggcttcct tctactttat ttaatattct tttttttatc ctttttttat
     1921 gaataacaaa aaaaagaaaa acattatgac agaaatattt tttgatacat ggtatattgg
     1981 tgttattaaa gaaaaggagg gaaaaatcat gcaatcttct gcagaattaa aacgccaagc
     2041 aaaagagagt ctaaaaggac gttggggaca agctgtttta ctaaatctaa tccctacatt
     2101 gattggtatt gctactattc ttcttattat gattcctatt atcttgtttg ccaccaccat
     2161 atatgatgga tcagcgagcg tctcagaagc gacaacttca ggaggcggta gttcaggagg
     2221 cggtggcgga attatttcca gcatcattag cgctttgttc atgagcggta tttcttggac
     2281 ttatcttgat attttacgtg gacaaaaaga tcagatcgaa ccattcaaag atgcatttcg
     2341 tggattcaaa ggtttgttta tcggcggagt gatcagctta gctgtgctga tctcgatctt
     2401 tacaacatta tggggtttgc tattagttat tcctggaatc attaaaagct attcttattc
     2461 tcaaagctat tttatttact acgatatcgt ccaagaaaca ggacaaacac ctaaagtact
     2521 ggatacgatc actgcaagtc gaaaattgat ggatggttat aaaggacaac ttttctggtt
     2581 agatctttca tttatcggct ggcatatctt agcattagca actgctggta ttggttatct
     2641 atggctcagc ccatacattt ctgcaacaaa agcagcattt tatgaacaat tgccaaaaga
     2701 tatttaaaag agagtgtgac ttgtgaccga gggggtggca ggagaacacc gtttatcgag
     2761 aggcagtaaa agaaacattc agcccattag caaaaaggag gtgtgacaac attcatgtca
     2821 cacctccttt ttgtatagtt ccagtaaaaa gaaaatcaac ttagaaacag ttaatcattc
     2881 ctcttcttct gcttccggta atcgatggaa cttgatcagt tcattcaccg cgattggtac
     2941 gatagataaa atccctacca agaaccagtg attcaaactg atgtgtacta ggccgaatag
     3001 ttcttgcgta aatggcagca aagcaacagc cgttgtaata gctaatgcca gtaacgccat
     3061 ttctaccaaa gctttattgg tcgctaaatt gactttgaag atcgattggc tgctgcgtac
     3121 attgaacaca tgcagaatcg atgaataagc taagatcaag aatgcaacag tttgaccgac
     3181 atgctgactt gcgtctaccc atgggctgat agtatcgaca taactaccta aataataacc
     3241 aaacaaggtt acgatggtga agacccctgc atttatggca attttttgca ataaacctcg
     3301 agcaaagatc ccttcatttt tcggaatggg tggctgatcc atgatgccgt gttccgctgg
     3361 ctctcttcct aaagcaaatc ctggaagtcc atcagcaact acattgacaa acagtagttg
     3421 cacagctgta aatggtgctc cccatcctaa cagcatcgca atcaaaacaa taaagatctc
     3481 cgaaatatta cagcttagca aaaagttgat agctttacga atattctgat aaaccgaacg
     3541 tccttgactg acagcgtcta cgatcgtcgc aaagttgtca tccgtcaaaa tcatatctgc
     3601 ggcactttgc gccacatctg ttcctgtgat ccccatggca cacccaacgt tacttgcttt
     3661 caacgctggt gcatcattca caccatcccc agtcatcgct acgactgcac ccgtacgttg
     3721 ccaagatttg acgatccgga tcttgtcttc tggcgttacc cgagcataga cagagagagc
     3781 tttgacacgc gcatctaatt cttcgtctga taattgatgc agttctgatc ctgaaagtgc
     3841 ttctgacgga tcattcaaga tacctaattc tttcgctatg gcactggcag tcaccacatg
     3901 atctccagtg atcatgactg ttttgatgcc agcctttttc gctcgcttga ttgctccttt
     3961 actttctgga cgtggtggat cgatcattcc gatcagacca atcaaacgta agtctttttc
     4021 cagtgcttca gaagaaatat cttgcggttc ttcgtcataa gtagcataac caacagcaat
     4081 cactctcagt gcccgtttac caaagcgatc attaacgatc gccgcttgtt ctacatcgcc
     4141 tgaactaaag cgcggagcaa gaacatcgaa tgctcctttg gtgattgaga tgtatttttc
     4201 gcccatttgg tgaactgttg tcatcatttt tcgttcagaa tcaaaaggaa gctcgtttac
     4261 tcgtggatat ttttcttcta attctgcctt tgtatgataa ttctcttcaa cagcccttac
     4321 gatggctact tctgttggat tacctttgat aacaaggtcg tcatcttctt gctcgaccgt
     4381 cacatctgta catagtgcag ccattttcaa tatttccatc gcgctgttcg tcattgaatc
     4441 ttcgatattc gttacttcat cttctttgct ccatacccgg cggacgcgca tttcattttg
     4501 tgtcagcgtt cctgttttgt ctgagcagat aacattagcg gttcctagcg tttcaactgc
     4561 tggcaactgt cggatgatcg catgtttcct cgccatcttc tgcacaccgt atgcaagtgt
     4621 caacgtaaca ataacagtca aggtttccgg tacagctgca acagccaagg aaatcgaagt
     4681 catgaacatc tcaaggagtg gttctccttg cagttcacca atcacaaata ctaaagccgc
     4741 agccgctaaa gcgatcatac tgattcgttt gcctagctga ttcaatcgga cctgtaaagg
     4801 tgttttttgc atagtatttt cgttcaaaag atccgcgatc ttacccattt ctgtagccat
     4861 gccaatggca gtgacgatcg ctttcccacg acctgcagca accgtacatc ctttaaatac
     4921 catattaagt tggtcaccta atgactcatc ttcttcagca ataaatgctg catctttttc
     4981 aactgcttca ctttctccag tcaacgccga ttcttctaca cgtaattgtg atgcttcgat
     5041 caaacgggca tcggcagaaa tagcatcacc ggactctaaa acaagaatat cacccactac
     5101 cagttgctct gactcaacag tggtcacttt gccgtctcgt atgacagttg ccgtttgttt
     5161 attcatatct tgcaaggatt ccagtgcttt ttctgcgttt ccttcttgga caatcgccaa
     5221 taccgagttg atcacaacga tagcgatgat cagcagtcct tcaaataaat ccccatgttc
     5281 tgtagcaaat gcggtataaa aggaaatcac cgctgctaca agaaggatga tcgttgtaaa
     5341 atcggacaag cttcgaaaga attttttgat catcgattct ttcggcgcct catcgaattt
     5401 attcaatccc tgcttttttc gccgttcatc cacttcttgt gaagacaacc cagtatctgg
     5461 atctgcttct gttaccttct ttacttcttc tattgtctgc ttgtattctt ccatcctatt
     5521 cctcctttaa aatcttcgta caaaaaaagg tacttatttt actataacat ccttttttta
     5581 tttacttcaa ataaagggct tatcgattat ttgttaagaa agcgtaaaat ttaaagtgct
     5641 tatatttgca aacgctacca aaggatgcta ttatatagat gtaaggaagg aggataccat
     5701 caaatagaca gaagaatttc tcgatagaac agtgtaagca aacaaagttg ttagaagtac
     5761 aggtttcacc cagacatgcg tgtagagtac aaacctataa atagtgaatg cgtatggatg
     5821 tttggatcac gaagtatccg aaacaagaag taatgcgata cgtaacaatt gaataacgta
     5881 aacgtctgaa aattgaatag gaacaagaga tgtttcaaat gttgaagatg taacgaaaac
     5941 aaaatttttg tttaacatga agaaattcaa caccgtgctt tgcatgtatg atggaaagtt
     6001 acaatcattt ctgtttattt gaatccttcc gaggtacaag aaagccgagc ttatcgagag
     6061 ctcggctttt tctttgtcat aaaatctttg taaagctttt ttgttattca aaactagcgg
     6121 cttttcataa ccttatttca cgaacatcgc atctccaaaa ctaaagaaac gatagcgctc
     6181 ttcaatcgca tgctggtaag cagataaagt caattctctt cctgcaaagg cactaactaa
     6241 catgactaaa gtcgattttg gcaaatggaa attagttgag aaagcttcta ccactttgaa
     6301 ttcatagcca ggcgtaatga aaatatcagt ccatccgctg tctgctttga tttctccatt
     6361 aaatttcgtt ccaatcgttt ctaatgttcg aattgaagtt gttccgacag ccacgatttt
     6421 cccgccattt ttacgtactt ggttcaatcg ctcagccgct tcttccgtca aacggtagaa
     6481 ttcactatgc atatggtgtt cttcaatttt atctacgctg acaggtcgga atgtacctaa
     6541 ccctacatgt agtgtcagat agactaattc aacacccttt gcttcgatct catcaagtaa
     6601 ttctttcgta aaatgcaatc cagctgttgg cgctgcagca gaaccatttt ctttcgcata
     6661 gacagtttga tagcgatctg ggtcttccag acgttctttg atatatggag gcaatggcat
     6721 ttctcccaat gattcaagaa tctctagaaa aattccttca tactcaaagt caatgatccg
     6781 tccgccgtgt tccaattctt ctttcactac tgcttgcaat ctgccatcac caaaagagat
     6841 tcttgtccca actttggcac gtttcgcagg tttgatcaat gtctcccacg tatctccttg
     6901 tgtattcgtt agaagaagga cttctaaatg cgcaccagta tctggttttt ctccatataa
     6961 acgagcaggc agaactcgtg tattattcat aactagcgca tcaccaggct gtaactcttc
     7021 aatgatttca tggaaatgtt tatcttccat ctctccagtt ttccgatcca aaacaagtaa
     7081 acgagaatga tcgcggtctt ttaatggtgt ttgtgcgatt aattcttccg gcaagtcaaa
     7141 atcaaaatct tctgttgtta acatatcgtc actcactttc tatcatgtgc ttcatcattc
     7201 tatcatgttt ttcgttctgt tggcaaaatc agcacttttt cacttctatt ttttgttttc
     7261 ctaaataaaa aaactaccct atttgtattt tttcattgtt ttaggcatac tagtaataga
     7321 tagttcttca ttgaaaatag aaaaagaggt ggcaccatga ttgaatttca aaatgtgtca
     7381 aaaatttata aaggtggaaa agtcgctgta gaggacgtca atctttcatt tgaaaagggt
     7441 gagttcattt gttttatcgg tacgagcgga agcggaaaaa cgacgacgat gcggatgatc
     7501 aaccgaatga ctgatccaac acaagggaaa attttgattg atggaaaatc catccaagaa
     7561 attgatccag tggaattgcg acgtcagatc ggctatgtta tccaaagtat tggattgatg
     7621 cctcacatga cgatccgtga aaatatcact cttgtccaaa aattgttgaa agtcagtcaa
     7681 gaagaacgga ataaaacagc tgaaaaaatg attgatcttg tggaattgcc ccgtgagatg
     7741 ttagatcgct acccacatga attatctggt ggtcagcagc agcgtattgg agtcgttcgt
     7801 gcccttgcag cgaatcaaga tatcatcttg atggacgagc cattcggtgc gcttgatcca
     7861 atcactcgtg attccttgca agaccttgtc aaagatttac aagagcgttt gggaaaaacg
     7921 atcgtatttg taacccatga catggatgaa gcgattaaac tagcaagccg tatcgccatc
     7981 atgagtgaag gccgtgtgat caatttgata caccagaaaa tattttacgt catcctgcaa
     8041 acgatttcgt agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga
     8101 caagagtcgg cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac
     8161 aagaagcaat caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt
     8221 cgaatgtatt aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat
     8281 ccagtgtcgg cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc
     8341 gtgatacctt gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac
     8401 agaacaaagt cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga
     8461 tttggggaga agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta
     8521 aggaggtctg acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag
     8581 gttttgaaca tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac
     8641 cgcttggtgt cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca
     8701 gtgccttgca gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg
     8761 taggtaaatt acctgcgatt atcgcactct ttatttattc attattgccg attttacgta
     8821 atacgtacat tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg
     8881 ggatgactga gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca
     8941 tggctggtat tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata
     9001 tcggcgctgg tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc
     9061 tcattttggc aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag
     9121 gtctcttgga acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg
     9181 tatactaaaa atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc
     9241 tggtcttgcc agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga
     9301 agcacagatc ttagcaagtc tagttgccgg aatggtttaa cattatacag ataaaaatac
     9361 cgcaatcatc aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga
     9421 tgcttcaatt tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc
     9481 tcctgaaaaa gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata
     9541 cggtcaaacg tggttcccgt cctatagttt tgaaaatacg tatgtctttc ttgttcgaaa
     9601 agacaccgca caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga
     9661 attagtagct ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt
     9721 ccaagaaact tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta
     9781 tgacgcagtc gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat
     9841 cgccagttat gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc
     9901 tgctccagtt gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa
     9961 taagttaggt cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa
    10021 taatctagtg gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga
    10081 agggaagtga agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag
    10141 gaaaatggct cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt
    10201 gtcctctttg cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa
    10261 ttagcaaact gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg
    10321 atctccattt taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt
    10381 ttgtattccc tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa
    10441 aacatccttg atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta
    10501 gagttaccac tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc
    10561 atcgggataa ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc
    10621 cgaggaacca atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca
    10681 ttaatggcga tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc
    10741 agccgtacat cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt
    10801 cgcaaaggct tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc
    10861 cttatgggca aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat
    10921 actgccaacg ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca
    10981 ttttgccaat ccattttccc atcactcgtc atggaattca taccacatga tctctaaata
    11041 ctcgtctgct tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat
    11101 ttaagccttt tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg
    11161 tgactgccgt atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat
    11221 ccttggctaa aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct
    11281 aaaaccggta cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg
    11341 atccttcacc agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa
    11401 gacattggcg taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat
    11461 ggccgaaagc gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt
    11521 cgctttggat acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc
    11581 ttttccaaaa gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt
    11641 gtccattgct tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt
    11701 cttcgcctca ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc
    11761 tccaatttta ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca
    11821 acattccatt cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta
    11881 aagaaatcat gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt
    11941 tggggagact ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt
    12001 gtccaggcag aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga
    12061 tgctgtctat tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa
    12121 gactgcgtgg gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc
    12181 tttaaattcg tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact
    12241 gtatagttac tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt
    12301 cgtctttttc gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt
    12361 cttgaatcgc tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac
    12421 taataatctc gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt
    12481 tacgagcagt gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata
    12541 taattatagc gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc
    12601 aaacttttcc tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca
    12661 cgctcacttc ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac
    12721 gtatcatctt tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac
    12781 acgaaggttc ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca
    12841 cgaaagattt gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca
    12901 aaaatgattg cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt
    12961 agaccgttgg gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat
    13021 ggtcacttga cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg
    13081 taaaggaaga agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt
    13141 atgatcaaga catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc
    13201 atacgctgtt cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa
    13261 acagaccctc tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt
    13321 tatttcttca aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc
    13381 aatcttaacc gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca
    13441 aattgaggaa tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata
    13501 tctacataac ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa
    13561 cggacatctg ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct
    13621 tcgtctagcg aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac
    13681 gcaccgatat ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa
    13741 tcatgtagtt ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca
    13801 tggatcttat aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc
    13861 cctaaccgat agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata
    13921 ttggcaatcg atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct
    13981 gtttgagata aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct
    14041 gttgagccat ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca
    14101 ttggaatagc ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt
    14161 gccagattgt atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa
    14221 atcagcgctg tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta
    14281 ccaatttgag acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata
    14341 aatgtgatcc ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg
    14401 gcattttttg ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca
    14461 gttaaagagg aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc
    14521 acagccgctc ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat
    14581 aaaccaacaa cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg
    14641 aacgatgcag attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc
    14701 acagaaatag cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca
    14761 cctgctgaga cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa
    14821 atcaagccag cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga
    14881 ggataagcat ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca
    14941 aaagcggcaa tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca
    15001 acgatttgtt ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa
    15061 gcagcaaatc gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact
    15121 aacaaccttt ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt
    15181 ttatagtcga aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc
    15241 aataaacttt aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg
    15301 ctttaataca gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa
    15361 aaggatagaa cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga
    15421 agagagatgt aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg
    15481 tcacaacctc ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa
    15541 attaaaaaca taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct
    15601 tttttacaac ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa
    15661 aacggcaaaa tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac
    15721 tgatttcaca acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat
    15781 ttcgtcattt tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca
    15841 aacaaaccga tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca
    15901 gattcccgta acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag
    15961 aatttccctg agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag
    16021 atgataatag cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc
    16081 atcatgaacg ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca
    16141 tctactccta gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt
    16201 gtcaaaaatg tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct
    16261 ttctttccta aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac
    16321 agacatagtg cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc
    16381 gcattggttt gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt
    16441 ttcaataatt tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct
    16501 gctgcttttt ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca
    16561 gcgttcacag ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg
    16621 atttcatttt ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt
    16681 tgctgaatga tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg
    16741 atggtatcca tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca
    16801 ggcatttctt tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt
    16861 gagctgagaa gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc
    16921 ccaatcgtat cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta
    16981 ttgataccag caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat
    17041 tcatcaatag ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt
    17101 tgcagattgc ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc
    17161 gtatttccag cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt
    17221 tgttgttgag atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca
    17281 tcatcagtca gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc
    17341 gttgttgcca cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt
    17401 atcgcatctt tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga
    17461 ttttctaaat ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct
    17521 tgttttgctt catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg
    17581 atctctgcta gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg
    17641 atgatttttg cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca
    17701 cccatctcat ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc
    17761 ttgatttcag gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg
    17821 tccaaatttt catctgttga taaaatcata tctttaactt tattgatact tactaggttc
    17881 gtatcgatgt cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg
    17941 ataaattcat ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt
    18001 gcaatcgcat tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca
    18061 ctagtaaaac tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac
    18121 tttccggaac ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat
    18181 tgtttatttt tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga
    18241 aactctgctg gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat
    18301 ggatcccata atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg
    18361 atgatcagaa gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat
    18421 cgaagcgtat ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta
    18481 ctttaccatg tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg
    18541 acatatcacg gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta
    18601 cagcctttac tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct
    18661 attatacgta tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac aaaaatgttt
    18721 cagctttttt cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc
    18781 cagcaggagg atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag
    18841 acagattcat tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag
    18901 caaaggcttc tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct
    18961 ttttttgccg caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt
    19021 tcatgatctg gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt
    19081 ctgcctgatc agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag
    19141 ctcctaattt ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc
    19201 ccttttcatg gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca
    19261 ttacagtatc tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc
    19321 ctggcgtgac cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt
    19381 ttgctgatgg caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc
    19441 gatcacctac atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat
    19501 cattacctgg tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca
    19561 aggcaacatc ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac
    19621 cgacttctct gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt
    19681 taattgcaaa tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct
    19741 ttattttgac aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt
    19801 cttttttcgc gtaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct
    19861 aataaaaaat aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa
    19921 aaaacagcaa gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa
    19981 agattacgaa ggcttgcgta aacttccttt agatgctcgt ccagaacgat tgaaaaatcg
    20041 tgatttaatc gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa
    20101 attccgtgaa ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc
    20161 caagtctggc tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa
    20221 agatacgttc gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta
    20281 cgcacagctg gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta
    20341 gtaacaggag tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc
    20401 ataatgggtc gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg
    20461 atctgctcca acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca
    20521 atgtgagtcg gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc
    20581 gggctaaaat ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc
    20641 tccgatccca cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga
    20701 tcctccttat tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata
    20761 gtggatcgta cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt
    20821 gccgataagc gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg
    20881 atccatacat acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc
    20941 ctgcatcttt cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc
    21001 ttcaggagtg atatatgttt ctccatagcc gattccttca cctgctggga gttttttgac
    21061 ttgaatcaat tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc
    21121 gtgcccagag ggattaagtc cgtacattgc tactccataa cgaatcatat tgccgattgt
    21181 ttcatcatgc cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg
    21241 aagttttttc agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc
    21301 ctgatctgct gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt
    21361 gatgaatgca gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc
    21421 agtgtctgct tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc
    21481 agctgctgct tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc
    21541 tacagtcagt gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc
    21601 ttctctcaac tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc
    21661 aactgctgcc tcagctgttt caatagctcc atgaccgtat ccgttcgctt ttactactgc
    21721 aaacaattct tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc
    21781 ttttttatgt atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc
    21841 tttctagtct tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac
    21901 ccatacattg ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt cattttttaa
    21961 aatctcgaca tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc
    22021 ctctttgcaa gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg
    22081 gaatagtttc atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat
    22141 cagccgagtg atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt
    22201 ttctcttttc aataaattta gaacactctc attctaccaa attataacca ttttgccaca
    22261 aataagtcca caaataacaa aa
//
LOCUS       DAZKNH010000043        22074 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000043 DAZKNH010000000
VERSION     DAZKNH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.043"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(268..1764)
                     /gene="lysS"
                     /locus_tag="ACG8MG_001501"
     CDS             complement(268..1764)
                     /gene="lysS"
                     /locus_tag="ACG8MG_001501"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFQ0375549.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1875..2879)
                     /gene="dusB"
                     /locus_tag="ACG8MG_001502"
     CDS             complement(1875..2879)
                     /gene="dusB"
                     /locus_tag="ACG8MG_001502"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFQ0375550.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2880..3767)
                     /gene="hslO"
                     /locus_tag="ACG8MG_001503"
     CDS             complement(2880..3767)
                     /gene="hslO"
                     /locus_tag="ACG8MG_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFQ0375551.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3984..6095)
                     /gene="ftsH"
                     /locus_tag="ACG8MG_001504"
     CDS             complement(3984..6095)
                     /gene="ftsH"
                     /locus_tag="ACG8MG_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFQ0375552.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6185..6730)
                     /gene="hpt"
                     /locus_tag="ACG8MG_001505"
     CDS             complement(6185..6730)
                     /gene="hpt"
                     /locus_tag="ACG8MG_001505"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFQ0375553.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6730..8175)
                     /gene="tilS"
                     /locus_tag="ACG8MG_001506"
     CDS             complement(6730..8175)
                     /gene="tilS"
                     /locus_tag="ACG8MG_001506"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFQ0375554.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8295..8765)
                     /locus_tag="ACG8MG_001507"
     CDS             complement(8295..8765)
                     /locus_tag="ACG8MG_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFQ0375555.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8811..9236)
                     /locus_tag="ACG8MG_001508"
     CDS             complement(8811..9236)
                     /locus_tag="ACG8MG_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFQ0375556.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9303..9572)
                     /locus_tag="ACG8MG_001509"
     CDS             complement(9303..9572)
                     /locus_tag="ACG8MG_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFQ0375557.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9583..11178)
                     /locus_tag="ACG8MG_001510"
     CDS             complement(9583..11178)
                     /locus_tag="ACG8MG_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0375558.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11193..14714)
                     /gene="mfd"
                     /locus_tag="ACG8MG_001511"
     CDS             complement(11193..14714)
                     /gene="mfd"
                     /locus_tag="ACG8MG_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFQ0375559.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14731..15291)
                     /gene="pth"
                     /locus_tag="ACG8MG_001512"
     CDS             complement(14731..15291)
                     /gene="pth"
                     /locus_tag="ACG8MG_001512"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFQ0375560.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15644..16618
                     /locus_tag="ACG8MG_001513"
     CDS             15644..16618
                     /locus_tag="ACG8MG_001513"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0375561.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16788..18188
                     /locus_tag="ACG8MG_001514"
     CDS             16788..18188
                     /locus_tag="ACG8MG_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFQ0375562.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18266..18988)
                     /locus_tag="ACG8MG_001515"
     CDS             complement(18266..18988)
                     /locus_tag="ACG8MG_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0375563.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19067..19573)
                     /locus_tag="ACG8MG_001516"
     CDS             complement(19067..19573)
                     /locus_tag="ACG8MG_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0375564.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19696..21495
                     /locus_tag="ACG8MG_001517"
     CDS             19696..21495
                     /locus_tag="ACG8MG_001517"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFQ0375565.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21563..>22074)
                     /locus_tag="ACG8MG_001518"
     CDS             complement(21563..>22074)
                     /locus_tag="ACG8MG_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFQ0375566.1"
                     /translation="TEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVT
                     PAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWT
                     VPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQW
                     FAPDFAVSM"
BASE COUNT         6111 a         4828 c         3993 g         7142 t
ORIGIN      
        1 ttgttatgtt tttgaataaa tattttcttc tgtcttttgg acaagtaata atataccaac
       61 taaatttagc ttggtcaacg aattttttgt attttttcaa gtttttttca ataaacgatt
      121 attcataact tttctttctt tatcgttcgt tttcgtcttc tttaatgtct agaatagtcg
      181 gcattagctt ttgaactttc gttattattt attcctttct acacagcaaa agctgcctaa
      241 ggaattcctt agacagcttt tattcgatta tctcattgtt gggaatagca aaacatcccg
      301 aattgattgt gcatcagtta gcaacattac taatctgtcg atcccgatgc ctagaccacc
      361 agttggcggc ataccgtatt ctaaggcttc caagaagtct tcgtctacac cgtgagcttc
      421 gtcatttcct agttcacgtt ctttttcttg cgcttcgaat ctttctcttt gatcaattgg
      481 atcgttcagt tctgtaaagg cattagcaaa ttcgcgcccc actatgaata gttcgaatcg
      541 atctgtgaat cgaggatctt ctggattttt cttcgctaaa ggcgaaactt cgactggatg
      601 tccgtataca aacgtcggtt gtgtcaatgt ttcttcaaca aatgtttcaa agaattcatt
      661 gataacatgt ccgacaccca tagcttcggt tacttctaca ttgtgttttt cggctaaagc
      721 taatgcttct tccaatgtca tttcttgcca gaagtctaca ccagtttgtt ctttgattgc
      781 atctaccata tggatgcgtt taaagtcact ttctaagtcg acagcttgtc catcatatgt
      841 gatttttgct gttcctagta ctttttcagc tgcgttacgg ataatacctt ctgtcagatt
      901 cattacatca ttgaaatcag tatatgctgt gtatgcttct aacatcgtaa attctggatt
      961 atgtgtagta tcaattcctt cattacggaa aacgcggccg atttcataaa ctttttccat
     1021 tccgccaaca attagtcgtt ttaaatgaag ttctagcgca atacgcaaat ataaatcaat
     1081 gtccagtgca ttatgatgcg tgataaatgg gcgtgcggca gctccacctg cctcattgtg
     1141 caatactggt gtttctactt caatatagcc attgccatct aaatagcgac ggatttcgct
     1201 gataatttgg cttcgtttca taaatcggtc aaagctttcg cgattactga ttaaatccaa
     1261 gtagcgttgt ctgtagcgtt gttcaatatt cgttaagcca tggtatttat ctggcagtgg
     1321 acgaagtgct tttgacaaaa tcgtgatttc tgaagctttg atagaaactt cgcctgtgtc
     1381 tgttttcatg atttgtccag ttacaccaaa gaaatctcca aggtctgcat gtttgaatac
     1441 ttcataggcc tcatcaccta cttggtcttt tcttacataa atttgaattt ggccttcacg
     1501 atcttgtaga tgcgcaaatc cagctttccc ttttcctcgg cgagtcatca tacgaccagc
     1561 tacactagcg gtcaatccca tttctgctag ttcttctttt gttcttggat caaatagttc
     1621 atgcaattct tctgagttgt gcgtgcgttc aaatcgtttt ccaaatggat cgattccatc
     1681 ttcacgcaat tgttccattt tttgacgacg tacaagcatt tgatcattta aatcttctac
     1741 ttgatgttgt tgttcctgtg tcacaaaagt tcctccattc tcatgttaat ctctttccta
     1801 taatgccaat gaaacacaaa aaaagcaagc cgaacttgga atttcggctt gaaaattcac
     1861 gaaaaactta gaaatcacaa cgtttgtttc aatagtactt tttccgcttt ttctacaaat
     1921 tgatccagca ggtcattgat ttcttgctgt ttttctgtct gattgattgc tactttcact
     1981 ttcgctgctc gaggaatccc ttttaaataa taagccgcat gtgttctgaa ttctctacaa
     2041 gctacttttt ctcctttgag atctgctaag cgttgtaaat gaagtttagc tgtagcaatt
     2101 ttttcggctg gtgttggttc aggaatcagt tcccctgttt cgagataatg ctgtgtgcgg
     2161 tgaatcatcc atggattgcc cagcgctgca cgaccaatca tcacaccgtc tgcaccgaca
     2221 tagtcaagca tccattttgc atcttctggc gttttgacat ccccgttccc cataaatgga
     2281 atcgtcaaat gttttttcac ttctttcaat acttcccagt ccgcttttcc ttcatacatc
     2341 tgaacccgtg tacgtccatg catagcgatg gcacttgctc cagctgattg tgctgctaat
     2401 gcattttcta cagcaaagac gtgctcttca tcccaaccaa tacgcatctt gattgttact
     2461 gggatattta ctgctgaaga aacagcttgg accatttcgt atactttctc aggatctaaa
     2521 agccattttg ctcctgcttc tgctttgatt actttattta ctggacagcc catattgata
     2581 tctattatat cggcagctgt gttttcttcg acaaattgag cagcttcaac tagactatct
     2641 ttatttccgc caaatatctg taaacttaat ggatgttctg tttcttcaat gtggagcatc
     2701 tctaaagttt ttttgtttct gaaatgaata ccttggtcac tgatcatttc acatacgact
     2761 aagcccgcac caaattcttt gacagtgact cgaaatgctg aatttgttat tccagccatc
     2821 ggtgcaacaa cgacacgatt agggatttcc acattgccaa ttttccatgt gtgttccact
     2881 taaatccctc ctaaggcttc tttcttcaat ccatctaatt cttcttcgct atacttgtat
     2941 ttgttattac agaaagaaca aaccgcttct gcaccgtgat cttcgtctat cattgcttgg
     3001 atttcttctg cacctaacgc gataattgct tctgcaaatt tttcttttga acagtcacat
     3061 ttgaattgaa caggcatttt ttccaaaatg ttcaattctt ctgtaccaag caatcctttc
     3121 aatatttctt ctggtgtctg tccttcatct aagagggtag agattcttgg tgtcgttttt
     3181 aattgttctt cgatcttgct gattgtttct tcatcggcac ctggcatgat ttggatcatg
     3241 aatcctcccg ccgtcttgat gctgtcgtca gtattgacca atacactcag tccaacagct
     3301 gaagggattt gttctgagat agcaagatag taagtaaaat cttcagcgat ttctccagaa
     3361 acaatcggcg tttgcccaga aaaaggttct tttaatccta agtcttttgt gacagtaaac
     3421 attccttgtg ttccaactgc tccccgcaca tcgattttac cttcactatt tagaggaaga
     3481 ctgagatgcg gatttttgat gtaacctttc acatctccct ttccatttgc atctactaca
     3541 attcctgttg ctggaccatc gccttggatt cgaacagtta gtttgtcttc tcctttgagc
     3601 gtcgcaccaa gtagcagact accgaccaat gtccgtccca aagcagctga ggaggtgtgc
     3661 caagtatcat gacgtctttg tgcctccgct acggtatttg ttgcttgaac ggcgtatgct
     3721 cggacgaatc cgtcgtgagc aagtgcctta accaaataat ctttcatttg ttatgtactc
     3781 ctttttccgt ttttaaactc atagtgaatc atactagcaa ctactgcttt tttcaacttt
     3841 ggaatgcttc ttttaaaaat aattcagaat catctagaaa acaggacatc accctattta
     3901 caacttaaat aaagaaatct cccgaaaacc ggtttttgtt ccggttttcg ggagtcatat
     3961 cagatcaatt gacctatatt tttttatttg tattgatcgt ctgaatgatt atcttctgat
     4021 tgttgagaat ctgaatcatg ttctgcttta ttttcctcat tcagttcatg ttttgcttct
     4081 tcaaaatcac gtttttcttc cgcttgtttt tcagcgtctt tttcttccaa agcacgtttt
     4141 gcttcttcaa aagttttcgc ttctttttcg cttggataat catttgactc agtaccttca
     4201 ggcatcacac cgtgttcaaa caatgacttg attgctttgg catccaatgt ttcgaattcc
     4261 aaaagttttt cagcaatcag tttatgctgt gcacgatgag cttcgatgat ttctcgagct
     4321 ttatcatgag cttccatcaa gatttttcgt acttcttgat caatctcgaa agcgacttgt
     4381 tccgagtaag cttttgtttg accatagtcg cggccaacaa acacttgatg gtttccttca
     4441 tattgaacag gacctaaacg atcactcatt ccatattctg ttaccatgct tcgtgctaga
     4501 gctgtcgctt gctcaaagtc attggaagca ccagttgatt ggacattgaa gatgatttct
     4561 tcagctgtac gtccaccaag taagcctacg atttgttcaa acatatcttc tttcgtcatc
     4621 aagaactgat cttcttttgg taaagcgatc atatatccgc cggcgcgtcc gcgagggatg
     4681 atcgttactt tgtggacgac ccgagcgcga ctcaatacta atccgacaat cgtatgacca
     4741 gcttcgtgat aagcaaccat ttcgcgttct tttttgctga ttacgcgatc ttttttagct
     4801 ggacctgcaa tcacgcgatc ttccgcttca tcgatatcag aagcatcgat tttcttttta
     4861 tttcttctag cagctactaa cgctgcttca ttcaagacat tttctaaatc tgctccggca
     4921 aaacctggtg tctgttgagc cacaactttc aagtcaacgt catctgccat cggtttattc
     4981 cgtgcatgga ctcgcaagat tgcttcacga ccttttacat ctggacgtcc aactaaaatc
     5041 tggcggtcaa aacggcctgg acgtagtaac gctgggtcta gtacatcaga acggttggtt
     5101 gcagcaatga cgattactcc ttcatttccg tcgaagccat ccatttctac aagcaattgg
     5161 ttcaatgttt gttcgcgttc atcatgtccg ccgcccatac cggcaccacg ttgacgacct
     5221 accgcatcaa tttcatcaat gaaaatgata gctggtgcgt ttttcttcgc tgtttcaaac
     5281 aaatcacgaa cacgacttgc tccaacaccg acaaacattt ctacgaaatc agaacccgag
     5341 atcgagtaaa acggtactcc agcttctccg gcaacagctt ttgctaataa ggttttacct
     5401 gttcctggag gtccttctaa aagtacacct gccgggatac gggcacctaa ttcaacaaat
     5461 cggcgaggat ctttcagaaa ttcaacaact tcaactagtt cttgtttttc ttcttccgca
     5521 cccgctacat cagaaaaacg tacgcgatta gcttttttgt ctgcttcttt ggcttttgat
     5581 ttaccaaagt tcatgacacg gccgccaccg ccaccgccgc cgccttgttg tcccatcatc
     5641 atgtaaaaga aaaagatgat gattacgatt ggcaagaagc taagcaatag ggaaacccat
     5701 ataccgctgc ttgattcttc ttttactgtt gtagcaactt tgttttcttt tgccagttct
     5761 gttacttggc tcaatgttgt atcatttggc aaaatgattg ttgtgaaatg ggtagtagtc
     5821 gtatctgttg aacctaagat agctaaccca ccgctgttag cgacggtctg ctgttctttg
     5881 tattcaccag tgatcttata tacaccattt gcaggctgca cttcaaattc tttgatcttg
     5941 cctgcttgta gttgttctgt gaattttgag tactcgatat ctgacgatcg ctggccgtta
     6001 ttaccaaaaa tgaaataaac gaccattacc atcgctaaga ttacgaggac ataatacaag
     6061 gcatttttca tcatgccgtt gttctttttg ttcatagttg ccctccctaa gcgtcaattt
     6121 aatttaacag ttttttatca atcctgacca ttctattgta ccataattta attgagatac
     6181 agcattaatt tgattgataa acactaggtt tcaatacacc gatgtacgga aggttacgat
     6241 atgcttctgc ataatccaag ccataaccga ccacaaattc atttggtacg ttgaagccaa
     6301 cataatctgc ttcgatattt actactcttc cttcaggttt gtcaagtagt gtaacaattt
     6361 tgactgattt tgctttgcgg tatttgaaaa gatccactaa ataagcaagt gttcttccgc
     6421 tgtcaatgat atcttcaacg atcaacagat cgcgaccttc tacattcgta tctaagtctt
     6481 tgacgatttt tacttcccct gacgagactg ttgcattccc gtagcttgaa acatccatga
     6541 aatccaattc caaatacgta tcgatagaac gaacgatatc tgccatgaat ggtaccgctc
     6601 cttttaagac acctactact aggggattgg tgttttggta aatttctgtt aactctttgc
     6661 ctagttcttt gcaacgaact tgtatctctt cttgtgagat caaaattctc tcaatatcct
     6721 tttccaacat caggttctcc ttccaattgc ttctttttga tatttgtaaa gaagtatgta
     6781 gtgtatttta tcagtttctt cacgaatact caaatatgaa aaggcaaaag gtatcaagct
     6841 atatatattt cttttggcat ctgtaatgat ccaagcatgc tgcctttgtg aattaggtac
     6901 tttctgatcg ataaaatatc tagatacttt ttttgtgaga ttctctgtta atttaatgcg
     6961 gtctcctgct tttctttttt cgacatacag ttgcacatca gcaggaatcc aaatgtcatg
     7021 agaaaataga gaccatgaat ctgttgcttt gatgtcccct gcattttcag gtttgaccag
     7081 accaatccat tctgtttcat tcaaatatat tccttgttct ggaatcagca acaagcgctg
     7141 gtcttctgtc tggctagttg cttgtttttc ttttttagag agtacaacaa aatgatactc
     7201 tcttttcagt atccaattat tgccaatact gagttgccat tggctttttt tctgttgcca
     7261 ctggtctaaa atcgactcca tttgttgttc tttcaaaata attcctgttt ttgagaatgc
     7321 tttgtcgaaa aaggagtaaa aaaaatagta tcgttcagca tctgtcattt cttcaatcat
     7381 ttctgcagaa aatcgaaatt gttcctccca ctgttcgact tccttttcga tcaactgtcc
     7441 catatatttt tgaatgactt ggtctgccca ttggatctgt tgcgaaaatt gctggaaatg
     7501 tctcaacgcc tgtgtatttt cttgtttcaa ttgaggaact accaaatgac gaagccgatt
     7561 tctctggata tctaacaatt ggtttgtctg atcctcaaag taaacaagct gcgattccgc
     7621 tgcataagta tataggtctt ctttgctaaa actcagcaac ggacgaatca gtctgcccgt
     7681 agcaaatggt tgtacttctt tgattccaga aaaagtattt aactgtcccc cacgaacgat
     7741 tttcattaac accgtctcga tttgatcatc tccgtgatga gcagtcatca acgtgtcata
     7801 ttgtccttct tccatcacgc gagtgaattc ttgataacga aagttacgag cttctgtttc
     7861 tatggcggat ttggctggat cttcccaaat acttacatat aaagggagat tcttctcttc
     7921 acaatatgag cgaagatagg cagcttcttc tgctgattct tctcttaatt ggtgattgat
     7981 gtggacgata cctaaagaaa aaccgatttg gcgttgcact tgtaaaagta aatggagcaa
     8041 aaccatcgaa tcaacaccac ctgataccgc tgctaaaatt ctcgatcctt gttgccagaa
     8101 tccttgttca ttcccgtgcc gaataaatga ctgctgtaat agcggtgctt ttttcccgcc
     8161 tttttcaacg ttcatccttt ccacaccctt tcttcctaaa aaaaagagag cgggaaaacg
     8221 tctaaaacga caattaacct gccctctgtt gatactatga cccaaatagt cgcactatca
     8281 ttatttgcgg ctttttagct gcgacgtccg ccacggccac cgcgctttcc ttctgtgttt
     8341 cgcttcaatg aagataagcg gtcatcgcta tctttcaaaa atgaactcat caatgaatcg
     8401 aagtcttctt tttgagctga aggttgactg cgagaaaaag attttttctg tgctggacga
     8461 tttcggttgt cacggggatc tttttgttga tattttttag gacggctttc ttctccagat
     8521 tcagcaggtt gttcctgcgc tttccggata gatagtccga tttttccatc atcatttact
     8581 gaaataactt taactgttac ttcatctcct acacttaaaa catcatggat gtcttttaca
     8641 tagccatttg acacttcgct aatatgaact agacccgttt ttccagcacc tagatcaata
     8701 aacgcaccaa aatttgtgat tcctgatact tttccttgca gttttgctcc tacctcgatt
     8761 gacataaaaa aaatgttcct cctaattatt tccctttttt atattcctgc ttattttgca
     8821 gttgtagaac tagttgattg atctgtttca tttgtcgagc tgtctgtatt tgattctaat
     8881 ataggataaa ctttttcacc gtcttttgag taaaagaatt tgcttctggc aagtttagct
     8941 acatactcat catctttcag caatgaaaca tcttggtgca gctgatcaac gtctgcgttg
     9001 acttctttca tttcagtatt tgcttcgact ctcaattcgt tcaattgatg catcctttgc
     9061 atgtcatgga aaatctgcac acctattatc gcgaaaatga ccaaagcacc aacaaaaatt
     9121 gcagccagtc tgcgtcttcg aaaaatcagt tgacgttgtt gttgctgaaa ttcagcatac
     9181 tttttctttg catattccgt atctagcgca gcgattttat tgcttttttt cgtcacttgg
     9241 attcgctcca atctttttga ttgtcttttt cattatacca atatcgtgaa agcttgtcta
     9301 atctatttct ctagtgtttc tgtgcgattt tcagaaataa tttcgtacat tttttgtgca
     9361 tcttcttttt tggttgattc atgtaattcc gtaattttca cttccaacgt tttgttgcca
     9421 aactggactt tcaaaatatc gcctactttg acatcactgg aagatttcgc caatttaccg
     9481 ttgatttgaa ttcttccttt atcagctact tcttttgcta ctgatcgtct tttgatgatt
     9541 cgggaaattt ttaaaaattt atctaatcgc atatcttatt cctcactttt taattttttt
     9601 atttaatcgc aggattttct taccaaaagg caacattagc cattctcgta ctgtaaacag
     9661 acgaagacga atcactgcat aaatgaacac agaagccccg atcccaactc ctatcacgct
     9721 gacaaaaaaa gcagtggaac gatgagttac aggaccaaat actatattca atacaccata
     9781 atacagcagc agtactactg tcatacaaag aaggctgaac atcaacttcc gtccaaaatg
     9841 ccgttctttc caaaaagcat ttagctcttt tgtttcaatt cgaacaaaat accaaagcgt
     9901 cacacctaat cctaaaaggg tagacaggct tgcacctgct gttcctaata aaccggtcag
     9961 aaaagaagta gtcaatcctt ttaccaatag tccccatcct gcgcctttta aagaagggcg
    10021 aaacgagttc ttgctttgag caatactttg ataagcttgg atgactgcag tgaaagcaat
    10081 ggaaaaaaca aataatacaa gcgctgcatt tcccgcataa tctttaaaca acgcataatt
    10141 gatataaggg agcagcaagg ctaatcccaa agacgcagct aatgctaaag cggtcgtcaa
    10201 tctaagatag attttggctg tctgtaaaaa ctgacgatag atccggttgg tcaaatgacg
    10261 agtcaacgca ggcaggaatg tcgcacttaa tgcagtggca atcactaaac ctaattgtac
    10321 taaaggctga cctcgatcat agaccccttt tgctatcttc gcaccgtatt ctgacagtcc
    10381 gtaaacttcc aaagcatttt ttacaaagaa agagtcgatc agctggaaaa agataagcaa
    10441 tccactataa atcgaaacca gtccgccttc taccagaaaa cgccggatca agggctttga
    10501 cggctgtttg aacaatggaa accgtcggaa gcttaacgct cctccatgaa tcttttgttc
    10561 atagtagtga aggatcccat atgcgcataa accgccaaaa acagctccac tcattgctgc
    10621 cgtccctgtt tggtacaccg tccatccaag ctttctaaaa gcaaacgcac tacagacaat
    10681 cacaaaaaca cgaacgaact gctcaactac ttgagaaaca gcagttggtt ccatcaagaa
    10741 tcttccttgg aaattccctc gataaaaaga tagaccaggc atcagcaaaa aggtaaagga
    10801 aactacttgg atcagcggtt tcaattgtat gtctcccatc atccaagcaa tccagctgct
    10861 aaatgcaaag acaaacaacc acaaaaagac acctgtccaa aatactaagg gataaagatt
    10921 ttgcaaagct tgctttcgct cgaaaggatt ctttctttct gcaacgtact ttgatatgaa
    10981 ctgcggaaga ccggataaag ctaaagtcat tgctatccca taaattggat aaacttgctg
    11041 ataaacataa aaaccttcgt cgccgactaa gttttggagg ggaattcggt aaagggcact
    11101 taatactttt gctatgaaag aagcaatcgt caatacaaat gctccttgca tgacgcgccg
    11161 catttcttta tgatccaaat cattgctcct tcctacgctg acagatattt ttgctcacgc
    11221 aacgctttga caaacttttg gatttcctga agccatactg cttcttccat ttggttgggg
    11281 atcgtcaagc gtatcaccat ctgttctttt tcaacgccta gactggcttt tagttttgtc
    11341 gctgtcagtg cttcgaataa ctgttccaca ctatagcgtt ttgttccaac tttacttaga
    11401 gtaaagacta tatgcgcctc ctgtttcctt atcgtttcta ggagcgctcg atcgccatcc
    11461 attttgatct gcccaattgt caagagatgt gctacttctt ctggatattc tccaaaacgg
    11521 tcgagcagat catcttctag ttcatctagc atttcttgcg aatcaagttc acgaattcgt
    11581 ttatagatct cgattttctg ccgttgatct gccacgtatg tttcaggtag atacgcatcg
    11641 acaccgaggt cgatctcaac agtggtcttc tccacttgac tattctttcc ttgtttacga
    11701 gaaacagctt cgcttagcat ttgagaatac atatcgaaac cgactgcatc aataaatcca
    11761 tgctgctgag cacctaagag attacctgca ccgcgaatgg aaagatcccg catcgcaatc
    11821 ttgaatccag atcccagttc tgtaaaatct ttgattgcct gaagacgttt ttcactgact
    11881 tcgttaagga tcttctgctg ctcatacata aagtaagcat atgcgacacg gctgcttctt
    11941 cctacccgcc ctcgcaattg gtataacgtg gataatccca tataatcagc gttctcaacg
    12001 aacaacgtat ttgcgttcgg gatatctact cctgtttcaa tgatggttgt agtgacaaga
    12061 acatcatatt gaccttcaat aaattcaaaa agcgtatttt ctaattgcgc ctctgtcatt
    12121 tgcccatgag cataaccgat ccgtgcttcc ggaacgagtt cttggatttc ttctactttt
    12181 tgttcgatcg tttctacccg attatataaa tagaatactt gtccacctcg tcccatttct
    12241 cgatggattg cttcgcgaat agccccagga tttttttcca taacatacgt ttggatcgga
    12301 taacgattcg caggcggtgt ttcgattact gaaagatccc gcacgcctaa catcgacata
    12361 tgcaaagttc tcggaatcgg tgttgcagtc agagtcaaaa catccacttg tgcacgtaac
    12421 tgtttcaatc gttccttatg ttttacacca aaacgttgtt cttcgtcaat gatcagtaga
    12481 cccaaatcgc taaattcaat atcttttgac aaaattcggt gggttcctac aacaatatca
    12541 acttgacctg tacgcagttg ttcgatggtt tctttttgtt gctttcttgt tctgaaacga
    12601 cttaatagcc cgatatttac ggggaaacct tcaaatcttt ccagcatcgt ttcatagtgc
    12661 tgctgtgcca agatcgttgt tggaactaaa aaggctactt gtttgctttc ctttatcgct
    12721 ttaaaggccg ctcgaagcgc tacttctgtt ttcccataac caacatcacc tacgagtagt
    12781 cgatccattg gtttttcttt ttccatatcc cgtttgatct ccgctgcact tctcaactgg
    12841 tcatcagttt cagagtaggg gaaggcattt tcgaattctt tttggtaggc gtcatcaggt
    12901 ccaaaagcat atcctttttc tgactctctt gcggcatata atttgattag atcgtcagcg
    12961 atatcctcga tttttgaaga gacttttcgc ttggttttcg tccattcact tccgcctagc
    13021 ttattgatgc gaggcgcttt ggcttcagag gcgacatatt tttggatcag attcagttga
    13081 gtaacaggaa taaacagttt gtcgtcgttt tgatacaaaa tagtcatata gtcttggtga
    13141 acgccatcta cttctagcgt ttccatccca atgtatttcc cgataccatg attggcatgg
    13201 acaacgtaat ctcctgcttt taactcatta tagcttttca atcgctctgc gtttgttact
    13261 gtttgacgtc tcgtttgttt tttcgtcgtt ttttggaaaa tttctttttc tgtgatagct
    13321 actatttttt cttgaggaag ttcaaaacca gcctgcaaag ctccttcaac taattgtacc
    13381 tgtcctctga tcaatgcatt tttttctgtt tctactacta atatgtcttc atctcgaagc
    13441 atttgttctg ctttgcgtat tctgtccttt gtcggaataa agatcacaac agtctgtttt
    13501 tgcttgcgcc aacggtccat ttctgttttc agcaatggca tttgtccgaa gaactgttgc
    13561 atcgtccgat attgaaaatt atggattgct tggaaacgaa gatttcccat ccctttttgg
    13621 aagagtgaga aaaaggttgt ggtgaacttt tccttctgca cgattttttg tatatctgct
    13681 gaaaatgtct gctcagaaaa cacccgcaat tcgctgattt tttgagtctg ccattctgcc
    13741 gcttcccttt cgatttcccg atttgtttcc atgattcgct gataatcatc aacgactagt
    13801 aaactatttg aagggaaata atccaagatc gtcacagatt cttggtaaag aagatctgag
    13861 tagaattttg cttcttctgt cgggacacct gcttgccagc ttgaagtcag ctgaccgaag
    13921 taatcttgca aaaactcgcg gtctgcttct tctgtggtcg cagcaagccg tttttctagc
    13981 attttttgca tttgctctat tccatgttct agatcctctg gtccgtagac ttgttcactc
    14041 gttggggaaa cccagatttc ttccgttttt tcgattgagc gttgtgtttc tgcttcaaaa
    14101 taccgcatag aatctatctc tacatcaaat agttctacac gaacaggata ggttgtcgtt
    14161 aacgggtaaa tatcgataat gcttcctcga atactgaatt ctcccggttt tgcgaccatc
    14221 tcttctcgta catagcccat caaagtcaat tggcgaggaa gattttccac gtctatctca
    14281 tcaccaatat gccaattcaa ttgcgcctgt agccaagttt tcttattcgg cagtcgtttg
    14341 tgcaaagatg cgactggcaa caaatagata cctgcttttt tttcttgaag tgcgttaagc
    14401 gttgcaactc tctcgcctcg cgcttctgga gaagaaaagg ccatttcagc agaaagcact
    14461 tcatccactg gaaataaatg gatctgatct tgcggaatca catgttgcag atcttctgct
    14521 aactgattcg cataatacaa atttggcatc agaataatca tgtttttatt tgttttctta
    14581 aacgctcgag caaatagaag tgttttcgct gagcccgcta atcctgtgat tagttgtcta
    14641 ggcatttttt cttctaaatt gctgacccat tcttttactt gcggatcttt tccgactaat
    14701 tgaataatat ccattccttt tcctccttcc tcaattgaat cgattcatcg catccacgaa
    14761 cgaatgccct tctatgagat attcgactgc tttaacagat tgacacatgc tttcttctat
    14821 caaaggctga ttttctttgc tgaaaggact aagcacatgc tggactactg tttttttccc
    14881 ttctggcctg ccgattccca ccttgattcg atcaaagaca ttcgtattca aatgggaaat
    14941 aatacttttg ataccgttat gcccgccggc acttcccttt tgacggagcc ggatttttcc
    15001 cactgccaga tccaaatcat cgtagattac gactaattcc tctggataaa tcccaaaata
    15061 agtcattagt gggccgactg cacgaccaga ttcattcatg aatgtttgtg gtttgactaa
    15121 aaggatcttt tctccattat gaaaaaattc ggcaacttct gcttcgaacg gatttttttt
    15181 gaaagtagct tgatgctcgc gtgcaatttt atccacgacc ataaagccga tattgtgttt
    15241 tgtatattgg tatttggtac ctgggtttcc taacccgacg atcattttca tttttgtttt
    15301 ccacctaatt ctaaaaagca gcacaaaagg ctggactgct aattagcggt ccagtccatc
    15361 gagctttttt cttttttaca cttgtcaatg catagtataa cacaaaacaa ggcgtttcgg
    15421 ctaaaagaga atcgttaata aatgataaag aaattaaaaa tttcttcaaa gtttaatgaa
    15481 ataattgata tataaaaata gtgaaataac gcacatgtaa gcttttacat ataatacaac
    15541 cttgttatct gttatatttt atagccaaat agcgtgacat ggggcttgag aaatgttagc
    15601 ataagcatgg gaaaaaacaa atcaatggaa ggattgatac taaatgactg cagctgcaga
    15661 aaaaaaagat catcaaaaag ttattcttgt tggagacggc gccgtaggtt ctagctatgc
    15721 ctttgctcta gttactcaaa atatcgctca agaagtaggt attgtggata tcaatactgc
    15781 taaaactgaa ggagacgcaa ttgacttgtc tcacgctttg gcatttactt cacctaaaaa
    15841 aatctattct gctacttacg cagacgcaca tgatgctgac ttagttgtta ttacagctgg
    15901 cgcaccacaa aaaccaggtg aaactcgttt agacttagta aacaaaaacc taaaaatcaa
    15961 ccgcgatgtt gtcacacaaa tcgttgaatc aggatttaac ggtatctttt tagttgccgc
    16021 taacccagta gatattttga cttactcaac ttggaaattc tcaggtttcc ctaaagaacg
    16081 cgtaatcggt tcaggtacct ctcttgactc agctcgtttc cgtcaagcac ttgcggaatt
    16141 agttgacgtt gatgcacgta acgtccatgc ttacatctta ggtgaacacg gagattcaga
    16201 attcccagtt tggtcacatg caaatgttgc cggcttgcaa atctatgaat gggtaaaaaa
    16261 caacccagaa atcgatgaag aagctatggt taacctattc tttggcgtac gtgacgctgc
    16321 ttacacaatt atcgagaaaa aaggcgctac tttctatggt atcgcagtag cattagcacg
    16381 tatcactcgt gccatcttag atgatgaaaa tgctgttctt cctttatcag tattcatgaa
    16441 tggcgaatat ggattgaacg acatctacat tggtgcacct gctgttatca accgtcaagg
    16501 tatccaaaaa gtaatcgaaa ttcctttaag tgacagcgag caagaccgta tggctgcttc
    16561 tgctaagcaa ttaaaagaaa ttctagatga agcttttgct aaactagacg ctgaataatc
    16621 tttgaacatg acggaaaaca tctattcggt gttttccgtt tttttatttt atcaccctta
    16681 tggtttctaa attttttcat aaagtagaga taaccttcgt cttttcctct aaattatgct
    16741 atcatgagac cgttccaccc taaactttta cttaaggagt tgtcgatatg acaagatctt
    16801 cgcatcgaaa aaaatcgaaa catgttgctt tgctttcagt tcttggtgtt ctcttagtgc
    16861 tgatcagctt ttatacctat cgcagtacat actacacaaa gcattttttg ccgaatacag
    16921 aaataaatgg aataaatgtc agcaatctaa cagtcgaaaa agcaaatgaa aaattaaaag
    16981 aagcttattc tgataaaaaa ctggctatta aagaagatgg aaaagtctgg caggaagtac
    17041 caaaatccga gctcggctac aaagatgatt tcacttcgga gctttctccg atcctaaatg
    17101 aacaaaacgg atggacttgg ggaatgactt atgtctcagc ggcagaaaaa caagaaatag
    17161 atcctgtcag tcaaaaccac caaaaactag acactaccgt tcagacattg acaactaagc
    17221 tgactgattt gaacaaagac cggacaccta ctactgatgc aacgatcgaa aaagcaggtg
    17281 actctttcgt tatcaagcct gaagtcaatg gaaatacgat cgacgtcgat gctgcggtca
    17341 aacaattaaa aagtgcggta aactctggta aagatacaat tgaattgact gaattcaaag
    17401 aaaaaccaaa agtaacatct gaagacagct cactgaaaga acaactcgca tccatgaatg
    17461 ctatcgcaaa tgtaaaagct acttacagta taaacggtga gactttccag atcccatcaa
    17521 gtgatattat gagttggctg acctacaatg atggaaaagt cgatttagat acagaacaag
    17581 tacgtcaata tgtaacagat ttaggcacaa agtataacac aagtacgaat gacacaaaat
    17641 ttaaaagtac aaaaagagga gaagttacgg tcccagttgg cacttacagt tggacgatcc
    17701 aaacagacag tgagacagaa gcattgaaaa aagcgattct ggcaggccaa gattttactc
    17761 gttcacccat tgtgcaaggt ggcacaaccg ctgatcatcc attgatcgaa gatacgtata
    17821 tcgaagttga tctggagaac cagcatatgt ggtattacaa agacggaaaa gtggcccttg
    17881 aaacagatat cgtttccgga aagccaacta cacctacacc tgctggagtc ttctatgttt
    17941 ggaataaaga agaagatgca actttaaaag gaacaaatga cgatggaaca ccttacgaga
    18001 gtccggtaaa ctactggatg ccgattgact ggacaggtgt tggtatccac gattcagact
    18061 ggcaacctga atacggtgga gacttatgga aaacccgcgg ttcacacggc tgcatcaaca
    18121 ccccaccaag tgtaatgaag gaattattcg ggatggtcga aaaaggaacg cctgtattgg
    18181 tcttctaaca aaaataatat ataaaaagaa agcacccgta cgattggtac aggtgctttc
    18241 tttttattat ctaacaatgt gctacttaca tttcagcagc agtaaagact tctgaaacat
    18301 catcatcgtc ttctagctta tctactagac gttccaattg ctctttttgt tcctcattta
    18361 aatcaatcgt cgtttgtgga atcatcgtca attctgcttg cgccaaagtg aactcttttt
    18421 ccaactcatc tcgtacagca gcaaaatctt ctggtgcagt ataaatctcg aatacttcag
    18481 gagaagtttg cagatcttct gcccccgcct ctaatacatc ctcaaacatc gtctcttcat
    18541 caacagtcaa cccatcgcgt tcaatagcga tatatccttt gcggtcaaac aagtaactga
    18601 ctgaacccgt ttctcccagt gatccaccgt ttcgagtaaa agctactcgg acatttgtcg
    18661 ctgtccgatt acggttatct gtcaatgcat ggactaagat tcctactcca cccggaccat
    18721 atccttcata cgtgatttca tcgtaattcg tttcatctac ggcactcgta gcctttttaa
    18781 tcgcccgttc cacattgtcg ttaggcatat ttgcagcttt tgctttgtcc acaacaagcc
    18841 gcaaagctgg attcatagat ggatcaggac cgcctgcttt agctgccata taaatttcac
    18901 gagaaagttt ctgaaagact ttccctcgtt ttgcatcttg ggcatttttt cttccttgga
    18961 tattatgcca tttactatgt cctgacatga tttttcctcc cttgttcttt tgagaataca
    19021 cttctattct agtactattt cacaaaaaaa caaagccaca gccaatctat aaacgcttct
    19081 tgaatgccgc aaaaagcaca cataatatca cagcagtcag agcaccgatc gaatccaaca
    19141 tgacatcttg gaacataggt gttcgtccac cggtcatcat ctgatggaat tcgtccaaac
    19201 ctgcgtaccc tgttgctgaa agccaagcaa ggatcgctgt cagccaccaa atttttagct
    19261 tggggaaaac acctaaaaac aagctgcctc ccaaaataaa ataggtaccg aaatgtgcgc
    19321 ccttccttaa aaagaactct aaaaatttgg cgtagcccag atggttgata ctcaccatac
    19381 ttccaccata attaaaacgt atacccgcta atgtgtcttt aaatggctga ttaggcagcc
    19441 agctttctaa tcgcgaaact tgtgattgct gtccgtaagt ttgagaagaa ctgtaaaata
    19501 agattataac gactatcagt gcgattacta aataaatatt tccgtttttc atttgttttt
    19561 ttgaagaatt cataagtcac ctctgtattt tatcttagac tttttttgaa gaaacaacaa
    19621 tcattttcat agagacttct tccataaaaa agtgctatac ttaattttgt attacaatat
    19681 gaagggagtt ttttcatgag ttattcatta aattggaatc tagattctat tttttccgga
    19741 ggcagtcatt ctgatgcttt gaaccaacgg atgaagcaat tggaagacca aatgaacgag
    19801 tattatcacc gcgtaacaaa atggtcacct agttctgaca atgcagagca gttaaacgct
    19861 attcttcaat tgcaagaaac gattacgaat ggttttaccc aatgcagcag ctatattact
    19921 gcattgcttt ctgccaatgt caatgattca gatgcaaagg tcttatcagg caagctttac
    19981 gcaatgctgc ctcgtctgca atcagccgaa actgtattgt caaagaaatt tgcagaaata
    20041 tctgacaatg attggaatca gatgctttct caatcttcgt ttgaaacaat tgcttttcgt
    20101 ttaaacgaga ttcgtcgtga tggctcacaa ttattatcag aagctgaaga aaacattata
    20161 aatacgcttt ctttagatgg tctaaatgct tggagcagtc attatgatac gattgtagcc
    20221 agcatttcga ttcccttcga acaagatgga caagtagtcg aattatctgc tggacaagcc
    20281 ttcaataaga tgatgggcga tccagatcca aaagtcagag aaacactttt cgcagcatgg
    20341 gaagaagcct ggaaagaaaa ggcacccctc ttcacggata ctttgaatca tttagatggt
    20401 tttagacttt ctgactatga acttcatggt gtcacagatt ttctacaaaa accattagag
    20461 tacaatcggt tgaagaaaga aacattgtct gtcatgtggg atacaatcca gaaaaacaaa
    20521 caaccccttg ttaattactt gactagaaaa gctaatcttt tcggtaaaga aaaaatggaa
    20581 tggcaagatc aagatgcacc tattatttta ggcgatttga aagaaaaaac ctttagtttt
    20641 gacgaagcag cagcatttat tatcgaaaac tttaacaaat tcagtccaaa aatggctaaa
    20701 tttgcccaat ctgcttttga aaagagctgg atcgaagcag aagatcgtcc tgggaaacgt
    20761 cctggtggtt attgtactga gttaccagaa acaaaagaat caaggatctt tatgacctac
    20821 agtaattctg ttaatgaggt tgcgacactt gctcatgaac taggacatgc tttccatagc
    20881 agcacgatgt gggatttacc ttctttaaat cgcgaatatg ccatgaatgt cgcagaaaca
    20941 gcaagtacat tcgcagaact gatcgtagcc gatgctacat taaaagtcgc aaaaacaaaa
    21001 gaagaaaaaa tcaatttgct agatacaaaa ctgcaaaatg ctttagctat gttcatgaat
    21061 atccattccc gattcatttt tgaaaatcga ttctatgaag cacggcaaga aggattagtc
    21121 tcagaagata agatcacaga gatgatggtt gaggcgcaaa aagaaggcta tcaaggagct
    21181 ttagctactt accatccata tttctgggct gcaaaattgc atttctttat tgatgacgta
    21241 ccgttttata acttcccata cacatttggc tacctcttta gtttgggcat ctatgcttac
    21301 gccaaccaaa agggagcaaa ttttgaagaa gaatatatcc atttactacg cgatactgcc
    21361 tcaatgacga cggaagatct tgcgaagaaa catttgaatg tcgatcttac ccaaccagat
    21421 ttttggcaag caggtatcga catggtgact gaagatatcc aaacgttcat ggaattgaca
    21481 gaagaattta tctgatttct attttatcac aataaaaaaa gagttagtcc agaatgggct
    21541 aactcttttt gattaatcat tcttacatgc tgacagcaaa gtcaggggca aaccattgaa
    21601 ctgaaccaac ttttacgctt tcgcctggtt gaggagcatg gatatattgt cctccgccta
    21661 aagcaatcgc tacgtggtaa gttccgcctt gtgaccccca gaataacaag tctccagctt
    21721 ttgcttgaga aactgaaatt tttgttccag ctgattcttg aggaactgtc caaccaccga
    21781 tgtcacgacc agttacttgc atgtaaacat agcgtgtgaa ccctgagcag tcaaagccac
    21841 ttggatcttt accgccccaa acatatggag taccaatata tttgtaagct tctgctacga
    21901 ttgctgcacc attcacactt ccagaaggag ctggagcagg tgttacaggt tttggttgtt
    21961 caggtgttgg tgttggcgtt ggagccggag tactacttcc tgtgccgttt cctgtatcaa
    22021 cactttgatc tgttgatggt gttgtaggcg caggtgttgt tgattcttct gttg
//
LOCUS       DAZKNH010000044        22006 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000044 DAZKNH010000000
VERSION     DAZKNH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.044"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..1432)
                     /locus_tag="ACG8MG_001519"
     CDS             complement(41..1432)
                     /locus_tag="ACG8MG_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0375567.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            1689..5078
                     /locus_tag="ACG8MG_001520"
     CDS             1689..5078
                     /locus_tag="ACG8MG_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0375568.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            5081..6502
                     /locus_tag="ACG8MG_001521"
     CDS             5081..6502
                     /locus_tag="ACG8MG_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0375569.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            6499..8376
                     /locus_tag="ACG8MG_001522"
     CDS             6499..8376
                     /locus_tag="ACG8MG_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0375570.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            8468..9310
                     /locus_tag="ACG8MG_001523"
     CDS             8468..9310
                     /locus_tag="ACG8MG_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0375571.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(9462..10337)
                     /locus_tag="ACG8MG_001524"
     CDS             complement(9462..10337)
                     /locus_tag="ACG8MG_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0375572.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            10544..11574
                     /locus_tag="ACG8MG_001525"
                     /pseudo
     CDS             10544..11574
                     /locus_tag="ACG8MG_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            11663..11968
                     /locus_tag="ACG8MG_001526"
     CDS             11663..11968
                     /locus_tag="ACG8MG_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFQ0375573.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            12147..14162
                     /locus_tag="ACG8MG_001527"
     CDS             12147..14162
                     /locus_tag="ACG8MG_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0375574.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            14164..14448
                     /locus_tag="ACG8MG_001528"
     CDS             14164..14448
                     /locus_tag="ACG8MG_001528"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0375575.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            14474..15847
                     /locus_tag="ACG8MG_001529"
     CDS             14474..15847
                     /locus_tag="ACG8MG_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0375576.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(15930..17945)
                     /locus_tag="ACG8MG_001530"
     CDS             complement(15930..17945)
                     /locus_tag="ACG8MG_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFQ0375577.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(17967..19181)
                     /locus_tag="ACG8MG_001531"
     CDS             complement(17967..19181)
                     /locus_tag="ACG8MG_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0375578.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            19401..20345
                     /locus_tag="ACG8MG_001532"
     CDS             19401..20345
                     /locus_tag="ACG8MG_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0375579.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            20641..21585
                     /locus_tag="ACG8MG_001533"
     CDS             20641..21585
                     /locus_tag="ACG8MG_001533"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0375580.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         7489 a         4011 c         4145 g         6361 t
ORIGIN      
        1 ttttatcagg gtggtttata catagctgtt ttttgattat tcataactcg aataagcgga
       61 tgagttcttc ttcatttgcg aatgacacac tatcatcaat caatatatga ggaacatggg
      121 cattcaaaat gggttgattc gtaacgatca gatcatactc gagaagattg tatttcgaca
      181 gctgatcttc agtaatagca gttgtctcag agtgaatttt atgcacagca agaatatcac
      241 tgatatattc ttcaatatat tgactatgtc gaagacttaa gtcgctgata atcagtattt
      301 ttttatcaga agtataatgg tgaatttcag gattaccgat aagcatccaa aagatatagc
      361 tagataaata ataaatcaga ttctgattca gtagagtgga ataaaattga agttctgcac
      421 gaaatccagc atatagacgt atctggcggt gttgcatctg ctgtgcaaag taagttgcac
      481 ggatcgtcaa atgagtcgta tcgtatggga aaatacggga gaaaaaataa atttgcgtta
      541 ataattgaac aacatattct tcgttttttt ctttgcaatc agcggatgaa aacgaacagt
      601 aggacatatt ttttcgtatc tgctcactga tttcattttt ttctttctcc gaaaattctg
      661 aaaaaatcgt attgacaaaa tagttcacta cttttatata gctgctgtac ttcaattttc
      721 ctaagagatt tttcaagatt aagaagtctt ttttatcgat atttatcaac tgtacataag
      781 agtctccagc tttccattca aatggagttc cttgggattc tctcatgaga tgcacaacaa
      841 aatacataat aaagaaagat tgctcatatg ggctttcttc ctgaaacaaa ttcatctgac
      901 aattattctt gaccataatt tctttgatct tctttttttc gattggaaat ggcaacgtgc
      961 gtcttccgta gtaacttaaa aagaaaaagg taacaaagat acgcaactgg agctcacttt
     1021 taaggctagt taactgaaaa ccttggtcaa cgatcacttt taataaatag ctttctaaaa
     1081 tttgattgca ttgtttcaac ttacgtgtaa aagaagaccg gctgatggaa attttttcac
     1141 aataatactc aatcgtttta cccggattga caataatctc atttaataaa cgactgtttg
     1201 tagaactttg cgacatttcc atcagaacat ttaaataagc tggtacattc aaatgttcaa
     1261 gacgataccc caatttttgt gaagattgaa tcgttacatc cgttccccag cggattttga
     1321 tctcttcgat ataccttaca aatgtagatt ctgaactacc aattcgtttg gcaagttctt
     1381 ttttagtcat ccacttgtca gaaatggaca gaattcgaat gattttttcc atctttctat
     1441 agtttaattc caacaacctg atcatatgtt tttctccctc aacccttttc tttcaatgaa
     1501 acgctcatta atcgaaggaa tcaacttcat aaatgaaacc aattggtatg aaaattggtg
     1561 tgattccctt tagcgccctt cgtataataa ttatcatatt aaaataaata aaattatgta
     1621 acgcctgttt attttaataa ttttcactat tcacgtcaaa acagaaaaaa agaaaaagga
     1681 ggggaaaaat gacgaccaca gggaagaaac tgaaagttat tttcatgctg ataatattga
     1741 gtttatcaaa ctttgtgcca ttatctgcaa tagcagacac tacagatgat ccaacagttt
     1801 tagaaacaat ttcagctgaa gtcatttcgg atcagtctgg aaaaaaagca ctgaacatca
     1861 agctaaatgc gaataacacc agtgctgaaa agatagaaaa agaaattggt ctagtcgaaa
     1921 attacttaag tgatgtggaa agaaaagaag gagatggcta tgcttatcag gtaaatagcg
     1981 ggaaaattac gttggaaatc tcatcaaaca ctaaacaaac tatcgatctg agttttccaa
     2041 tcgatccagc actttaccac agccaggcaa acaagctgat cgtcgataat aaagaatatg
     2101 acattattga tgagacagaa aataagaaag atacagatgt gtcagtacca aagccagacg
     2161 aaatagaaga agaatcatca aaagaaaacg aaaattctgt cagcccattt acattgccta
     2221 cattatcctt gccagctgtg agtgtgccat ctaatcaaac gattcctaca gaatatacaa
     2281 cagatgatca gggcacttat cctaaagcca gttggcaacc tacaggaaat acaaatgttc
     2341 ttgatcatca aggcaataaa aacggaacaa atcaatggga tggtataaat tcttggaatg
     2401 gagatcctaa tgatcggacc cattcgtata tcgaatatgg aggaaccggt aatcaagcag
     2461 actatgcgat acgaaagtat gcaaaggaaa caagtacacc cggattgttt gatgtttatt
     2521 tgaatgctcg tggaaatgta caaaaagata tcacgcctct tgatctcgta ttggtcgtag
     2581 actggtcagg aagtatgaac gacaataatc ggatcggtga agtaaagatt ggtgtcgatc
     2641 gttttgtcga tactttagca gatagcggta tcacagacaa aatcaatatg ggatatgtcg
     2701 gctactcaag cgaaggatat agctacagta acggtgcagt acagatgggt tcatttgatt
     2761 cagtgaaaaa tcaagtaaaa tccattacac cttcacggac aaatggtggt acttttacac
     2821 aaaaagcact aagagatgca ggaagcatgc tatccgttcc aaatggacat aaaaaagtga
     2881 tcgttttgct gacggatggt gtaccaacat tttcctataa agtacagcgg gtacacgcac
     2941 aatcaagcag caattattac ggaactcagt tttctaatac gcaagatcgg ccgggaaata
     3001 cttctctaat ctcaagaatc tatgatgcac ctgaccaaaa caatctatcc agaagaatcg
     3061 acagtacgtt tatcgcaacc atcggagaag cgatggcact caaagaacga ggaatcgaaa
     3121 tacatggtct tggcatccaa cttcaaagcg atccggcagc tggtctctca aaagcagaag
     3181 tagagtctcg tatgcgacaa atggtttcat cagatgaaaa aggcgatctt tactatgaat
     3241 cagctgatca tgcaacagat atctctgaat acctagccaa aaaagctgta cagatctcag
     3301 caactgtaag caatggacaa ataaatgatc caatcgcaga accattcatt tatcagcctg
     3361 gtacactttc agtcaagagt gtggggacaa gtcctacaac ggtcactcca tctatttcca
     3421 tagaaggaaa taccatcaag agcaatcaga tctatttagg aaaagaccaa gaaatccaaa
     3481 tccattacca agtgagaatc caaacagaaa atgaggactt ccatccaaat ttctggtatc
     3541 aaatgaacgg caggacaact ttccagccaa acattgatac caatgaatta gctgaattcg
     3601 gtataccatc tgctaaagct cccggagtca gtcttcacat caaaaagtta tgggaagaat
     3661 ttgacaacaa tctagctgat cgtccagatc aagttacttt tgagattcaa cgggaacata
     3721 cgacaaatgc tgcagcttgg aaaaacggat atattcgaat cattaaacca gctaaagata
     3781 caacaaatac gtgggaacgt gcagacattg acaaattatc tgcaaatagc ggagaaagtt
     3841 atcaagagat attatcacta cctcaataca ataatcaagg tcaagcattc agttaccaaa
     3901 caatcaaaga attacctgta ccaggatacg attctcaaca aatagatgca atgacatgga
     3961 aaaatactaa acaattcaca ccgttaaact tgaaaataac gaaaaattcc tctacaggtg
     4021 aaaaggatct tattggcgct gttttcaaat taacaggaga ttctattgat actttactaa
     4081 cagatcatgg cgacggaacc tattctcttc cagaaaatgt caaattgcaa aaagaaatga
     4141 cctatacgct gacagaaaca aaagctccag aagggcatgg attaagcaaa aagactactt
     4201 gggaaatcaa gatcgcttct gatggtacgg taaccattga tggaaaaaca gtcactactt
     4261 ccgatgatac gatccagttg actattgaaa atccttttgt tgaagttcct gtagcagtac
     4321 gtaagtatgc gatgcaaggg acggacaaag agataaatct taaaggagca gcattttccc
     4381 tacagaaaaa agaagcaaat ggtacttatc agccaattga cagccaaaca acgaatgaaa
     4441 aaggtcttgc cagttttgat tcactcacac ctggtaaata tcgagtcgtt gaaacagctg
     4501 gtcctgccgg atatgatact tcgccgggaa attatgaatt ccaaatcgat aaatatggaa
     4561 aaatcattta cacgggaaaa aataccgaga tgacaaataa tgtatggacg ctcactcatc
     4621 aaaatcgact aaaagcgttt gatctaacgg tacacaaaaa agaagacaac ggacagacat
     4681 taaaaggagc aaaattcaga ctgcagggac cagaaatgga cttagaatcg ccaaaagatg
     4741 gacaagaaac agataccttt ctattcgaaa atttaaaacc tggaacttat acgctgaccg
     4801 aaacttttac accagaagga taccaaggtc taaaagagcc agttactata gttatacacg
     4861 aagatgggtc aattcaagtg gatggacaag atcatgaatc tgttctgtca ccaggagcca
     4921 aaaacaacca gatttcttta gacatcacga atcaggcaaa agtaccatta cctgaaacgg
     4981 gaggaattgg ccgtttagga atctatctag tagggatgat tggttgtgcg ttttctattt
     5041 ggtatctttt tttgaaaaaa gaaagagggg gcagctaaat atgaaaaaac ttggttggct
     5101 tagtatgtgt ctcttcttgt tactatttaa accagctttt actcaggtag caacagaaac
     5161 agaaacagaa atggttcaga ttactttaca caaattgctt ttcccaaacg ggcaactgcc
     5221 gaaaaatcat ccaaatgacg gacaagaaaa agctttatta caaacgtatc gaggattaaa
     5281 tggtgtcaca ttccaagttt atgatgtcac agattctttt taccatctac gggaaaaggg
     5341 caaaacggta gaagaagcac aagcagagat cgcaaaaaac ggtgcgtctt ccggtatgtt
     5401 taccgcagaa gcaacaacta caactcttaa caacgaagat ggtatcgctt ctttttctct
     5461 ggccgctaaa gatcaagaaa aaagagataa agcgtatctt ttcattgaat ccaaagtacc
     5521 agaagtcgtc aaagaaaagg cagagaatat ggtagttgtt cttcctgtac atggacaaaa
     5581 caatcaaaaa ctttcaacta tccatttgta tcctaaaaat gaagaaaacg actaccctga
     5641 tccacctttt gagaaggtat tagaagagcc tagaaatgat tttacgattg gtgaaaaaat
     5701 cacttattcc ttgcatacga caattcctgt aaatatcctt gactatcaaa agttcgaatt
     5761 gtcagatagt gcggatgaag cattaacgtt tttacctaat agtttaacga tttcatcgaa
     5821 tggagaaaag ctgacagaag gctttgtcat acacaagaaa cctcacggat ttgatgtttt
     5881 attttcgatc ccttcgttgg aaaaatatgc tggaaaaaaa ctgaccattt cttatcagat
     5941 gcagctaagc agtacagcac aggcgaacaa ggaaatcaac aacaacggaa cactggattt
     6001 tggttttggt gtcagtacaa agaaagtctc tgtatataca gggagtaagc aatttgtcaa
     6061 aatcgagaca aataaaccag ataaacgatt agctggcgca gtattcctta ttaaaaacaa
     6121 agcaggaaat tacctccagc aaacagccaa cggatacaag tggacaaaga acgaatcaga
     6181 tgcgcttcac ctgatttccg ataaaaatgg cgctttttca atttccgggt tgaaaacagg
     6241 aagttatcga ttaaaagaga tcgaagcacc ttctggttat attttaagtg aaacagaaat
     6301 tccgtttacc atttcaactt ttctttctga ggataaagag gcggacagta tattgaaagt
     6361 agtcaataaa aaagaaaata gccgtccatt tcttccaaaa acaaacgaaa cgaaaaatac
     6421 acttttaggc gttgttggta tggtattcgc aagctttgca atctggttgt ttatcaaaaa
     6481 aagaacagga gtgaaaaaat gaaaaatcat aaaaaaataa acgttatgtt aggagtcctt
     6541 ttccttattt taccattact cacaaacagc ttcggcgcaa aaaaagtgtt tgcagaggag
     6601 acagcagctc aagtcatcct tcataaaaag aaaatgactg atttacccga tcctttaatc
     6661 caaaacagcg ggaaagaaat gagcgaattc gatcaatacc aaggattagc cgatatttca
     6721 ttttcagttt ataacgtcac tcaagaattt tatgcgcaac gagataaagg agcgtccgtg
     6781 gatgcagcaa aacaagcagt ccagtctttg actcctggta caccagttgc ttcaggaacg
     6841 acagatgctg atggaaatgt cactttatct ttacctaaaa aacaaaatgg gaaagatgca
     6901 gtctacacga tcaaagaaga accaaaagac ggagtgtcag ctgccgcaaa catggtttta
     6961 gctttccctg tatatgagat gatcaaacaa gcagatggct cttataaata cgggacagaa
     7021 gaactagata ctatccatct ctaccctaaa aatacagtcg gtaatgatgg aacgttgaaa
     7081 gttacaaaaa tcggtactgc cgaaaacgaa gcactaaatg gagcagaatt tattatttct
     7141 aaagaagaag gaacaccaag cgtcaaaaaa tacatccaaa gtgtcacaga tggattgtac
     7201 acttggacaa ctgatcaaac caaagccaaa catttcatta ctggtcattc ttatgacatc
     7261 ggcaacaatg actttgccga ggcatctatt gaaaaaggcc agttgatcgt taatcattta
     7321 gaagttggaa aatataattt agaagaagta aaagctcctg ataatgcgga aatgattgaa
     7381 aagcaaacaa tcacgccttt tgagatcctg gcaaatagcc aaacaccagt agaaaagacc
     7441 atcaaaaatg atacgtctaa agttgataaa acaacacctc aattgaatgg aaaagatgtc
     7501 gcaatcggtg aaaaaattca atatgagatt tctgtcaata tcccattagg tatcgctgat
     7561 aaagaaggaa cgcaaaacaa gtacacaaca ttcaaactta tcgatactca tgacgctgct
     7621 ttaacatttg ataatgattc ttcaggaacg tatgcttatg ccttatatga tggaaataaa
     7681 gaaatcgacc cagtaaatta ttctgtcact gagcaaacag acggattcac ggtttcagtt
     7741 gatccgaatt atattccttc attaactcct ggcggtacat tgaaattcgt ttactatatg
     7801 catttgaacg aaaaagcaga tccaaccaaa ggattttcta accaagcaaa tgtcgataac
     7861 gggcatacaa atgatcaaac accaccgtca gtcgatgtcg ttactggggg caaacgattt
     7921 gttaaagtag atggtgacgt tacatcagac caaacacttg ctggagcaga attcgtcgtt
     7981 cgtgatcaag atagtgacac agcgaaatat ttatcgatcg acccatccac aaaagccgtc
     8041 agctgggtat cggcgaaaga atcagcaacg gtttttacaa ccacaagtaa cggtttaatc
     8101 gatgtgacag gtctaaaata tggcacgtac tatctggaag aaacgaaagc gccagaaaaa
     8161 tatgttccat taacaaaccg tgtagcattt actatcgatg aacaatctta tgtaacagca
     8221 ggacagttga tttctcctga aaaaatacca aataaacaca aaggtacact tccttcaaca
     8281 ggcggtaagg gaatctatgt gtatatcggt gcaggagtag tccttctact gattgctgga
     8341 ctgtactttg ctagacgcaa gcacagtcag atttagtctt tttgcaagaa ttggaaaaag
     8401 gagcagcgga ttacgcaaat gctccttttt cttttattat caaacagtca tcacaaggag
     8461 aacacaaatg aaaccaaaga aaaggaaact tctagatctg ctgatgcttt tcttgcttct
     8521 ctcaggaata gggatactag cttatccatt tgttagcgac gcgctgaata attatctgga
     8581 tcagcagatc atctctcatt atcagcaaca agcggtcaaa gaaaatgaag aagtcatggc
     8641 taaaatccaa gaaaacatga caaagaaaaa ccagcaatta gcaaaagaag gtggaaatcc
     8701 aggtgcagat ccctttacta agaaaaaaga acctgcgaaa aaggatagaa cctattttga
     8761 gaaacatacg atcggtattt tgacgatccc tgagatcaat gtccatttgc caatttttga
     8821 tgaaacgaat actgtgctgc tagaaaaagg tgcctcatta ttggaaggaa cgtcttatcc
     8881 tacgggggga gaaaatacac atgccgttat ttccagccat cgaggactgc ctcaagcaaa
     8941 gttgttcaca gacttaccga aactgacttt aggtgattct ttctatatcg aaatcaatgg
     9001 acggacactc gcttatcaag tcgatcagat caagacggta gaaccgactg atacaaaaga
     9061 tctgagaatc gaaaaagggc aggatttcgt gacactccta acttgtacac cttatatggt
     9121 caacagtcat cgcttacttg tcagagggca ccgggttcct tatcatgcaa aagaagttca
     9181 aaaagaagta cagaaagtat ccgagaacaa acgatttttc ctgtatggat tgttagctgc
     9241 atgtatactg attttcctcc ttcttatata tctttttcgt caatcgttca aaataagaaa
     9301 cagaagttag ctattcctca tttctataat atatctattc gaatcgttcc gtaaacaaac
     9361 aggctgaggt ttgaaaaaga aaacttcagc ctgtttttat gatgatatca aagagagcct
     9421 atcaaacact atctttctta ttactaaatg aggatctttt cttaagtttt ccgatattct
     9481 ttaagaaggc ctttttgcat caattcttgt ttgtttttca agttgcggat attctcttca
     9541 aaattttgag aatatatcac tgagaataac gtatttaata cgtaatcgaa ggcaatttga
     9601 gaggagaaag tcgctacttt agcaaaatca tattcctcct gcgatataac aagacacata
     9661 tcagcctgtt tggccatttc tgaatgctga tttccggtaa tcagcaaggt aggggcctct
     9721 acatgcttca aataggtcat tattcgttca taatgatgga tgcgaccgct gtagctaata
     9781 aaaatggcgc aatcctcgct agtcaaattg gctgcattcc aagaagaatc actgtattct
     9841 tcagccatga tcaaaaactt attcaacttg accattttat tctggaattt ccttgccgtg
     9901 atttgagaat caccttttgc aaaaagaaag attcgctgtg ccttgttcaa tactttaccg
     9961 atttgatcta aatcttcatg ttcaatctgc aattgtgctt tttttattga ctggatcgat
    10021 aaatccgcca ttttctttgc aatttgctga gtgctatctt cacttgaaaa aggaaaatta
    10081 ggatcaatcg tttgattgaa ctgagcaatc aattgatgaa cttcccgccc cagctcgaaa
    10141 cggaattctt taaatccgct gaatcccatt tttttgcata aacgaatgat agctgaatgg
    10201 gaagtatacg tagcttttgc taattcttca atcgtcatgt agactgcagc ttcgatattc
    10261 ctacggatgt aatcgacaat ccgtttctct gtcgtggtaa acaactcttg ttttgttagt
    10321 ttttcttcaa taagcatgat ctgacctcca aattctatct tactgataaa cacttggttc
    10381 cgctactatc tttttgattt ttatccttac tttgaacatt aatttcacca agccgaatga
    10441 taacaagtgt ttttctgaac ttttttacca gaatagctat tataaatact ttctgcgctt
    10501 ttctaggata tactgaaaac atcaataaaa ggaggcacca ctcatgctta caatcgctta
    10561 tcttggcaat ggaaagagta caaatcgcta tcatttgcct ttttcaagaa aacttacgaa
    10621 caaaatcaat atcaaaaaaa ttttttcccc atctgaacca gtgtggaaaa cattcgaaga
    10681 catcgaatat actgatcagc tgtctgatct ttgggatgat cctgagatcc aattagttgt
    10741 catcactaca cctagtcaat ttcattatga atatggaaaa atggcactcg aacatggtaa
    10801 gaatgtacta gtagagaaac cttttgcaga aacgtcaaag gaagcagaag agttatttgc
    10861 attagcaaaa gaaaaaggtc tatttatcca atgttaccaa aacagacggt tcgattctga
    10921 cttcttgaca gtccaaaaag taatcgaaag cggaaaatta ggggatattt tagaagtaga
    10981 aatgcattat gattatttca gaccggaagt accagaaaat acgcatgaat tttctactgc
    11041 gaacagctat ttatatggac atgcttgtca tacagtagat caagttcttt cttatttcgg
    11101 cgaaccagat gatatcagct atgatgtacg tcaactttta gggaaaggac gaatgaatga
    11161 ttacttcgat ctggattttt tctatggaaa tatgaaaatt tcgataaaat ccagttattt
    11221 ccgcatcaaa gaacgtccaa gtttcgtcgt ttacggaaaa aagggatgtt cgtgaaacaa
    11281 acaaaagatc gacaagaaga acatctgaaa atgttttaca tgcctgatca tgaagacttt
    11341 ggtatcgatt tgcccgaaca ttatggtgta ttgacctata tagatgaaca agggagttat
    11401 catgaagaaa aagtaatttc cgaggttggt gattacagcc gtgtctacga agggatttac
    11461 gagacattag ttaatggcgc agaaaaagtc atcaaagatg aagaaacaat cttacaaatg
    11521 aaaattttgg aagaaggaat caaaagcatt caagaatcag aaggaaaaat atgacatatt
    11581 tgtaagaatc gggtttgttt tttgacgcat aaactgtctt attgtacgat gaaaaagtat
    11641 agaactacta ggaggaacca acatgacaaa ttatgacggt gaaaaacaca aacgattaag
    11701 aggttcaatg gcccctaaaa cagaagaaga agtggcttta gcaggaacta attctggtga
    11761 aacagaagac aaagaagaat cattagctgg cacaaactct ggtgaaacag aaaataaaga
    11821 aacagcttta tctggttcaa attctgtaga agaggaaaca aagcctaatg agcccaatca
    11881 ctttgaaaaa gatatcgaaa aagcggttga agatgtcaaa gaaaaatttg aagaactgac
    11941 ctcaaaaaat aaacatgatc atcattgatc ggacgaaatc aattcataaa agcaggagac
    12001 atttggtcac ctgctttttt tctgtatcca ttcgatagaa atcgttcggt acagtcgtaa
    12061 aaaaaatcca actgtaagtt gtaccaaaca cccctcgatt taaaagcgct accatactag
    12121 aatataagta ggaggtgaac agcgctgtga actacaaaat tagttatctc ctgaaaacac
    12181 caagagaaat cagtctagaa aaagcagcag agatcatcga agtaaataca agcgaggcga
    12241 tcaaacaatt aactcctttt gccgaatatg tggatgatac ccgtatccgt ataaagaaat
    12301 tccgagcaat cgactgggca gaaaaatttt tggatattac tcgatttgaa gctgtgtata
    12361 ccgaacaaga gcgaagagat cttctttatt tgatggttta ttcgactgca acgtctgttt
    12421 ctgtttttca ttttcaagat tttcttcagg tttctaaagg gacagtcctg tcagatatca
    12481 aaaagataag aaatgaatta gaagtggaag attgtcagct gtcgtatact aggaaaactg
    12541 gttttaggct gctagggtca gaagcaatat taagaagaga agcaaaaaat cgcactgctc
    12601 gtttgatgca gtcacttgct ggaagatttg ggctcacgta tttaaccgca accctttcca
    12661 ttgattatta cgccaaagtt agagatttgc tagataaaac gatttcacta tcagatcttc
    12721 gattcgcacc aaatcgaatg gatgaattag cttattattt gagtttgaca accattcgaa
    12781 ttggaaaaac atccatggaa ctggtaaaag atgctgaact gttggcttct cttgcagtca
    12841 ctgacattag tcgacaagta ttgactggcc ttggctattc cgaacatcaa aattcggaga
    12901 ttatattttt ctctctttgc ttagcatcga tcgttgaagg agatgtccag gagcctgcac
    12961 ttgatttttt gctggaatgt tcttcagaag tcatccatcg catggaattc ctgatggccg
    13021 tagaatttgc ttcgtttcgt gaattattga tgaatgtctt ttatcacttg gtccctgcct
    13081 attttcgtat ttcctattct ttttatttac ctaatgtgat gatcgatcaa attaagcatc
    13141 aatatgcttc catctacgaa atgacaagaa aagcattact tccgttagaa agacgaatag
    13201 ggaagagcat ccctgaagaa gagattggtt ttttcactat actatttggc ggagagatca
    13261 gaaaagcgga cgcagaagaa agaaatagaa aaataagagc cgtgatcgtc tgtccaagtg
    13321 gcattagctc ttcattgatc ttaaaaagtg aacttcagca attattcccg atgatctttt
    13381 ttacagagac taattcagct gaccgcttac acgaggttga cgaaacttct tacgatatta
    13441 ttttttctac tgtacctctc acacttactg ataagaaaaa gtctctctat gttctgcatc
    13501 caatcatgac tgacttggaa aaaaatcagc tgatcaatca agtacaaaga gattggctga
    13561 taccaggatt ttcaatgcct tctgcccaag aattgttgtc ggcgctactg ccttatatcg
    13621 aattaaaaga aggcgtagac gaagaaaagc tctatcaagt attaaatcgt aaaatgaaca
    13681 aacttttaga aaagaaggag gatcaacgtc ctatgctatc tgaattatta acagaagaaa
    13741 tgatccaatt atcggatcaa ccaaaaaact ggcaagaagc aatcacattt gccgcacagc
    13801 cgctattatc agaaaataaa atagaagaaa gttatatcaa agctatgatc gagcgcgtgg
    13861 agcagtatgg tgcgtttatc catattggcg atcacatcgc attacctcat gctcgtccag
    13921 aagatggcgt aaacgaagta gggatgtctt tgctgaaact tcaacaacct gtggatctgc
    13981 ttgataatcc agcccatccc attagtttgt tcatctgttt agctgctatc gataatgagg
    14041 cacatcttcg ggctttggca aatttaacaa agctactttc taataaacaa aatcttcaag
    14101 atttattaca cgcatcaaca aaagaagaaa tattagcgat cataagaaaa ggagacgaat
    14161 gatatgaaaa tttttgcagt atgtcaaagt ggattaggaa caagttttat ggtagagatg
    14221 aacatcaaac aagcacttgc agatcttgga gcagatgtgg acgagtttga agtaagtcat
    14281 acggatgttg gaagtgtatc tagcgatatg gctgattatt tcttcattga gcattctttg
    14341 aaagatacgc taacttctat acctgatgaa aagctcgtac ctttgcaatc gatcattgac
    14401 agcgaagaag taaaagaaaa agtaacgaag atcttgtcaa aagaataaaa atgattcaag
    14461 ggaggaacta tcaatggata aattgttgaa tttgtttgtg tcaatcgtca gtcagccctc
    14521 catcctcgtt tctttgattg cactgcttgg tctcgttctt cagaagaaga aattttcaga
    14581 agtgatccaa ggaactatca aaacatttgt cggattccta gtactgatcg gcggtgctgg
    14641 tttaatcagt aattcactaa ccccttttgc ttcgatgttc caaacagctc tgcatactca
    14701 aggagtagtt cctagcaatg aagcagtagt ggcgattgct ttgcaacaat acggtacacc
    14761 tactgctttg atcatgctag tagggatgat cgtgaatatt ttacttgcta gattcacaag
    14821 atataaattc atttttctta caggtcaagc aatgctttat gtttcttgtc taacagcagt
    14881 cattttagta agcgcggggt ttactgcaaa tttgtggatg atcttattag gcggggtctt
    14941 cgaaggaaca ctattaacta ttacgcctgc tctttgtcaa ccgtttatga agaacatcac
    15001 tggaaacaat caagttgcgt tagggcatac cggaaatatc ggttatgctt ctagtggatg
    15061 gatcggaaag ttttttggaa acaaagaccg ttctacagag gacataaaga ttcctaaaag
    15121 tttcagcttt ttgcgagatt ctactgtatc gatcatgctc ttgatgtcga tcgtatatgt
    15181 cgcacttgct ttgttagctg gtcctggatt tgttgaaaga gaactaagca atggagacaa
    15241 cgctgtaatc tttgcactga tccaagctgg aacattcaca gctgggtttg ttatcgtcct
    15301 tcaaggggtt cgaatggtgt tggcagaaat cgttccagca ttccaaggga ttgctaaaaa
    15361 actcgtaccc gattctaaac cagcattaga tgttcctatc gtttttccat atgcacctaa
    15421 tgctgtatta attggctttt ttgttagctt ctttgtagga acactatcca tgtttgctat
    15481 gataggcttg caaactacag ttatcattcc tggggtagta ggacatttct tctgcggagc
    15541 tgcttctggt gtcttcggca actcgactgg cggtagaaga ggggcggtat taggttctgc
    15601 tttcaatagc ttattgatta gttggctgcc attatttatc cttccagtat taggagatct
    15661 taaaatcgct tcttctacat ttgccgatac tgattatcta gtaccaggac ttgtacttgg
    15721 gaaactagga gaatacggtc aaatagcctt gacagttgga atcatcgctt tctttttact
    15781 tgtcgtatta tctagctttc tttttgccgg aaaaagaaca aacattgttt ctaaagaaca
    15841 gaattaagca aacttcaaaa taaaaattgc ttggacaata tcagaagaag atttaaagtc
    15901 ttctgatatg tcgcaagcag ttttttgttt tatgcattta tttttgaata gcttgttaag
    15961 acaagtggct gttttgctag aagaggataa cttttaccat tcattttcac tgttccgcct
    16021 gttctatcag aagtaatcac gatcttatct tttgtttttt caagcgaaat ctttccatct
    16081 ttgataggta gcgtacaatt cagtgtttca aatgaatcta gattaggttg tatcataaat
    16141 tcttcataac ctggctttgt tggatacaaa ccgataaaat agcgtcctaa aagatagatt
    16201 gggcttgctc cccatgcatg gcataagctt tttccataag gatctccata catactgtac
    16261 ttttcatttc ctgtttgagt agggtcgtat tcttcccaga aagttaccgc atcatgagat
    16321 aacatccctc cccaataatc aagaatcgtt tggtagacga tattcgtctg tccgagtttg
    16381 cataaagcat cttgttcata gaatttgaaa tatggagtag taatctgtgt gatagtgtca
    16441 ttcaataaga cattctttaa gatcgattgc tgttggtctt tgttagcaaa atcgaataaa
    16501 acagcgaaaa tattcgcatg acgcgtcaca tttcttctcc cagattcata gcaatcgata
    16561 aaagcgccaa gttcttcatc ccaaaagtag tcaaataggt tttgtttcaa tagttgatat
    16621 ttttcttgat agagatgagt atgataattt tcgttctctc catatgtttc agctaagata
    16681 tctccgcaag tgatcatgga atgataggct ttcagtaata agacttgttc tgcagcaagc
    16741 gttccttctt tatccatttc agaccaatca ataaatatcc aatcttttgc tctcccataa
    16801 ataaacccta attcattcgt ttgctccatc aagtatctca ttaatgattg catcttaggg
    16861 tagacgatgc gtaaaaattc atagtctttc gtcatcatat agtggttttc gataccaatc
    16921 aaccacaaga tcgaatagtc aacgatagta ttcatatgct gcttgatttc attttgtcct
    16981 cttaatgcta atatcgttcg tttattgatt tcctcgtcaa aaaaaagata ttgattgatc
    17041 atgaatgatt gatacgcatc tccagaccaa atccagcgat ctcgtttgat accgtcgata
    17101 aaaaacaaat cactacataa cttgaaggct tcttctgata ctgaccagat ctgattcagt
    17161 aaagaagaat cgcttgaaaa agcagcttgt ttcgaaagag ataaaaattc atgaaaaact
    17221 tgaagttgaa ccattccagt agaaacctct ggtataaaca aatagcgaaa agcgcgttta
    17281 cgagctttca tttttcttgt cattcctatt tgcttatagt agcaataatc gatatccata
    17341 gcttctacat cggactctcc ataacataga gtgatagatt cgatatggtc atccagagat
    17401 attagttcta cacagccatt cactgctcgc ccaaaatcga ataagattcc tccgccaatc
    17461 tccgtttcag tgactggatg gacgcattct ttttgataat agatttgatt tggatctttt
    17521 gcgatatcga cataaagcgg actccagcca gctggatatt cttcaataaa attacttgct
    17581 gtccagccaa tatctgattt tacttcgtct ccctcgataa acatcgctgg tagccctgat
    17641 gtatgtccta caaagatacg aatttttgct ttaccagctg gacaagtcag ccattttcca
    17701 catggatgtt tttgtccatt aatttctaca tacccaactc cttggatcgt cactttaaac
    17761 atggaaggtt gatcaagaaa gtaatatctc ttaaacttga cgttccgatg gcaatcatcc
    17821 atgtaccagt acgctggcca gtcaaaacct cgttcttccc gttggaaatt ttgtagcagt
    17881 ccatggcgga tctcaaagtc tccaggatac caaagccatc tatctttatg tttttcattt
    17941 gtcattttac acgacctcca tcaaatttat tttgttgtca attgttcttt tatttcttca
    18001 tctactctta tcactgtctt tctattcgtc ctgtgtacta ctagaaggct aataagcgcg
    18061 ctaatcaaca gcaagatact gccattgacg aaaacagcaa gtgttttact cccaagagaa
    18121 gcaaaagaga gaaagagcaa accatatggt gcaatgaaag tacctaaatt agcgccaacc
    18181 aataagattg aagtattgaa actctccgcg tacttgggcg cttggatact tacttggttg
    18241 aataagtaag gaatgaataa cgaaaaagaa attcctgtta ggatcgcacc aaacccaatc
    18301 acatacaggt ttatcacagt ggcaatcaat acaaagccga ttcccatcag tagtaaagaa
    18361 agtgtaagca tatagcgatg tgcttttgtc tgaagccagc caaaacaaac tccggcagcc
    18421 atacttgcca attgcatgat tgttaagata ttcgctgcat tagtttcgtt tcctagagaa
    18481 taagtcgtca ggactgtggc taatttaatc gatacagcat tatataccac gatgaccagc
    18541 aagagaaaaa taccataact aatcatttta ggattcgttt tgattacttg tcttgttggg
    18601 gctgtgccat gcgattgaag ttcagttgtt tcaggtgtag gaacaaacaa aacaaacaag
    18661 acaagaatag gtaagctgat ggcgtagact aaaaaggtat attgccaacc gaactgtact
    18721 agctgtcctg caacaaatgt cattaaagct gcaccaaatc cttgaaatgc actttgaaaa
    18781 ccaatcatag aggctcgttc atctccgtga taaaaggcag caatcatgct gactgccaaa
    18841 gaattgatca atccaaagcc aatacctagt ccagctcttg aagctaagac aatactgtaa
    18901 ctttgtgaga agagggggag gataccacaa atcgatgcaa tcactaatcc gatgatcaca
    18961 gtttgttttt gtccgatttt cctagcaata aaactactaa tcaaaacaaa aatagtgacc
    19021 ataaaagagg gagcagtcgt taataattca acagcagata aagaacgcgt ggcataagta
    19081 gctgacatac tgggaattgc agacgtgact gcaccagcag aagtcaaaat aagagagact
    19141 gacagcagag aaaatttcat catcttttta ttcataagca tgttcctttc atttttgtaa
    19201 gcgcttatca aaaatatagt atgaagtggt tattctctca atagcggaat cacaggaagt
    19261 tcatgtccca accatagaaa gtctttatga aaccaaaaat agctttgcat ataatagcag
    19321 tacagcgtga actaataagc gaaagaaacc gcttacaaaa aggagggagt caattggaac
    19381 tagctgaatt agacattttt ttgcgtaacg aagaatcaat cgaaactctg cagcgtgaac
    19441 aaggaatgaa tatcaatgat ttaaacttag cttaccatct tcagacaccg ccaaaaatcc
    19501 accgagggga tctatttcaa cagggaaata ttcatatcag taaacaccgt cgatatgcgg
    19561 aagttcccga gcatacacat gattttgttg aaatcaatta tatgtatagc ggtacatgtt
    19621 tacaaattat taatggagaa aggatagaga tggaagaaag tgacgtggtg atcattgata
    19681 aagaagctac tcaaagaatc gaatacacag ggcaagagga tattttaatc aacatcttat
    19741 taaaagaaga aaccttatct acagagatat tgaatcattt ggcaagcaca aataatctac
    19801 tttcttcttt tttgataaat gcatcaaaag aaaaagggga acatacacaa ttcattcatt
    19861 ttcgcggcgg aaaaacaagt cgtttgacgc atttgattga aaatatgctg ttgatttatt
    19921 ttgatacata cagtcataag atccgttcgt taaatctcta tacatcacta ttactgatcg
    19981 aattgactca tttattagaa caacaaagct tttcatcagc agatggatgc gcagaaaatg
    20041 agatgcttag agtattagat tatatcgata agcattttac ttcttgtacg ttaaaagaaa
    20101 cagcagctca ttttggctac aatccggtct atcttagtaa taaattgaaa atgtattttg
    20161 atgcttcctt tcaagaactg gttctaaaga aaaaattcga ctttgcgttg gaactgattc
    20221 aagaaacgag tttttcgttg gaagaaatcg ctaatcgtat cggatataaa caaacgactt
    20281 ctttattcaa attattcaag aagtattcaa catgtactcc caatgaatgg agaaataaac
    20341 attaagaaga aaatctttca ctaaagcata tgaaggaaaa ttgttttagg tgcgattgct
    20401 gtcttattat atcgttgcag cgaatttttt tggatttatt ttcagttcta aacttttggt
    20461 tgtattatca ggggatatag gtgtaaacat ttgtcattta tctgttatgc gtattcagga
    20521 attcgtttcc atcaaaaatt ataataacaa ctattataat taacatgttg ataaaatgaa
    20581 taaacaagac tatacttttc atgaaagtaa caaatagtta tgaaaaggag ctgtttattc
    20641 atgaaaaaaa caagtcgtaa agtagtaatc gtcggtacag gttttgttgg aacaagtatc
    20701 gcttatgcaa tgatcaacca aggaatctct aatgaattag tattgatcga tgtgaatcaa
    20761 gaaaaagcag aaggggaagc attggacctt ttagatggta tggcttgggg cgatgaaaat
    20821 gtggctgtat ggtcaggtgg ctatgaagaa tgtaaagatg ctgatatcgt tgttatcaca
    20881 gctggtatca accaaaaacc tggtcaatct cgtttagatt tagttaaaac aaatgcgtca
    20941 atcatgcgcc aaatcgtaaa agaaatcatg ggatcaggat tcgacggtat tatcgttgtt
    21001 gcttcaaacc cagtagatat tttgacatat atcgcttgga acgaatctgg tctgccaaca
    21061 tcacgtgtta tcggtactgg aacaacattg gatacaactc gtttccgtaa agaaatcgca
    21121 ttgaaattaa aagttgaccc acgcagcgtc cacggctata tcttaggtga acatggtgat
    21181 tcagaagttg ctgcatggtc acatacaact gtcggtggta aaccagtgtt tgaaattgta
    21241 gaaaaagacc atcgtattgc ccaagatgaa ttagatgtga ttgctgataa agtccgtaat
    21301 gctgcttatg agatcattga tcgtaaaaaa gcaacttact atggtattgg tatgagtact
    21361 gcacgtatcg taaaagctat cttgaataac gaacaagctg tattgcctgt atctgcttat
    21421 ttaactggtg aatacgacga aaaagatatc tttacaggtg tgccatcaat cgttgacgaa
    21481 aatggtgttc gtgaagttgt tgaattatca atcaacgaag aagaaaaagc aatgttcagc
    21541 aaatcaacaa gtgctttaag agaagtattg aacactgtat tataagaaac aatgaatatg
    21601 aactaaaaat cctaaaactg cttaacagct gaaatagctg ttaagcagtt ttttattgat
    21661 atctaatagg taagcaaata aatagaatgg ttaaaattaa tcggtccgaa aatgtatccg
    21721 tacatatgaa catatgaaat ttaatctgtt gattacaata agcgaatcgt tatcaatgtt
    21781 tatctgtggt taattaaaaa atccttccgc aacggcaaat acaatgattc atatctaatt
    21841 cgttcatatt tgattaactg aataaagaaa ctggttttta tcattcaatt aaacgacaaa
    21901 aaaagtaaga aaaactgcaa aattttttta taattttgca taagcagatt ttgatggaaa
    21961 aatgacgatt tttgataaaa aaccgttcga gagaggttta tcattt
//
LOCUS       DAZKNH010000045        21711 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000045 DAZKNH010000000
VERSION     DAZKNH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.045"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(180..1079)
                     /locus_tag="ACG8MG_001534"
     CDS             complement(180..1079)
                     /locus_tag="ACG8MG_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0375581.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1089..1445)
                     /locus_tag="ACG8MG_001535"
     CDS             complement(1089..1445)
                     /locus_tag="ACG8MG_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0375582.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1800..2096
                     /locus_tag="ACG8MG_001536"
     CDS             1800..2096
                     /locus_tag="ACG8MG_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFQ0375583.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2740..3693)
                     /locus_tag="ACG8MG_001537"
     CDS             complement(2740..3693)
                     /locus_tag="ACG8MG_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0375584.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3716..4471)
                     /locus_tag="ACG8MG_001538"
     CDS             complement(3716..4471)
                     /locus_tag="ACG8MG_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375585.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4468..5427)
                     /locus_tag="ACG8MG_001539"
     CDS             complement(4468..5427)
                     /locus_tag="ACG8MG_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0375586.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5420..6370)
                     /locus_tag="ACG8MG_001540"
     CDS             complement(5420..6370)
                     /locus_tag="ACG8MG_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0375587.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6520..6972
                     /locus_tag="ACG8MG_001541"
     CDS             6520..6972
                     /locus_tag="ACG8MG_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFQ0375588.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7061..9328)
                     /gene="gshAB"
                     /locus_tag="ACG8MG_001542"
     CDS             complement(7061..9328)
                     /gene="gshAB"
                     /locus_tag="ACG8MG_001542"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFQ0375589.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9515..10513)
                     /gene="ftsY"
                     /locus_tag="ACG8MG_001543"
     CDS             complement(9515..10513)
                     /gene="ftsY"
                     /locus_tag="ACG8MG_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFQ0375590.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10524..11333)
                     /locus_tag="ACG8MG_001544"
     CDS             complement(10524..11333)
                     /locus_tag="ACG8MG_001544"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0375591.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11342..14923)
                     /gene="smc"
                     /locus_tag="ACG8MG_001545"
     CDS             complement(11342..14923)
                     /gene="smc"
                     /locus_tag="ACG8MG_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFQ0375592.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14943..15629)
                     /gene="rnc"
                     /locus_tag="ACG8MG_001546"
     CDS             complement(14943..15629)
                     /gene="rnc"
                     /locus_tag="ACG8MG_001546"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFQ0375593.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(15977..17758)
                     /locus_tag="ACG8MG_001547"
     CDS             complement(15977..17758)
                     /locus_tag="ACG8MG_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0375594.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17784..18698)
                     /locus_tag="ACG8MG_001548"
     CDS             complement(17784..18698)
                     /locus_tag="ACG8MG_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0375595.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18725..19687)
                     /gene="opp4B"
                     /locus_tag="ACG8MG_001549"
     CDS             complement(18725..19687)
                     /gene="opp4B"
                     /locus_tag="ACG8MG_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0375596.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19687..20631)
                     /locus_tag="ACG8MG_001550"
     CDS             complement(19687..20631)
                     /locus_tag="ACG8MG_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375597.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20632..21636)
                     /locus_tag="ACG8MG_001551"
     CDS             complement(20632..21636)
                     /locus_tag="ACG8MG_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375598.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         6067 a         4640 c         3844 g         7160 t
ORIGIN      
        1 caaaaaaaga atacacgctt gcatctagta ctctgcgagt tacaaatcat ccgatcaaat
       61 aaacattcgc tgattatttt ccgcaaaaaa agcagcatat ccacgataaa tggatctact
      121 gctcttactt atccttatgt tttacaaggt attttctttt acacattact aatgctgtct
      181 caatacggaa cgattgtcat cgcttgttca aattcctctt gcaaatgttg tgtttgatag
      241 gcctgaaaag ctgggtgatg ggcacgcaag aggacacgta atttcatagg atcatagctg
      301 tctagcaact gttctttcaa gacaaaatct cgggtcagct gccattcata ataataacca
      361 aaaggtctgc agctgtaaat cgtgtgaccg atggatttat cttcaaagcg acgatgcgta
      421 tggccaaaaa ctacttgttt gatatttgaa taacggtcaa gtaattctcc gaatgattct
      481 gatcctaaaa atgcatttaa tttgttccaa cgctcataag gagcagattg atacatgata
      541 aatgcccgtt taggaacgaa atgagtggat aaaatcacct caagtttttt tgcttccagc
      601 tggtctagca aattttttgc agctgcaagc atttctgcat tgacctcgag atctgttttc
      661 ccacgctgga tgaaccgatc ataccaatac aaacgcttca ttcgtacgat tgcctcgtaa
      721 tctttcaatt cggaaaactg atagtcgtac cagccattca tccctaaaag aacggtctgc
      781 tcatttaaag caatataccg ttcatttaaa aaatgtgggt ctggaaaatg gtccatcatc
      841 tgttcattgg gcacatctgc caactcgtga ttgccgaaat gaaatgtcgt agataatcct
      901 ttattgacga aaaattcgac aacagctagg caacggtcta ctttattagc cgtatctccg
      961 gctagatgaa gccgagtgat cttgtgcttt tgcaaaactt gccaaagctg catcagctct
     1021 gattcgccga atttattgat gtccacatgt aggtcgctga tgatcgccaa ttttcccatt
     1081 ttatccctct agtcttttcg aacaacttgc aagttgcctt ttctcaagcc gcttggtcct
     1141 tttaattgta aactattata agttggttct aaagcaagtg tggatggatc atccatgtaa
     1201 agtattgcac tgtctttgat tttgattggt ccaactggtg tatcagccac tacagaataa
     1261 agacttagat ctgtttgtgt atcttttttg acaatgatca atcgaaatgc cgtatccaat
     1321 tggcaactga cttggctttc tgcaaatgga ccgtcaccgt tttcgatatc taataaaagt
     1381 tgatcgtctg cttctaattt attcttcaat acttcttgtg cttcttttgt cacttctaat
     1441 ttcatctgta tcacctcatg tcatagaata atcactttat tcatcagatg caactaattt
     1501 gtctggttct gactgttctc aatttattgg aaagtaagtc attcgtttat tatgatttct
     1561 cttatcacaa aagagacaac tttccctttt cttcgttcag tttcttgaac ggaaaaagac
     1621 ccaaaaagca acctgttatt tgtttaacct tattcctttc ttaaataaca aaaaatacta
     1681 taaaaaataa aaaaccttta acaactattg gaaaaagaat tactttttgt tcacggatta
     1741 ttaacaactc ttcggtagag taaacttatc aaataaaaga cttctttgga ggaatcacta
     1801 tgaaggaata tataaaaaac atctatttta tcgaagaaac acaaaatatc gagggttctt
     1861 atattgaagt aaaaacactc tttgtcaatg aagataaaac caaagcattg gatatttata
     1921 aaaaattagc ttcaaaaaaa acgaatagtt ttggattgat attaagcgag tataaaatca
     1981 aagcagaaga gagttatttc tatcaattac taaaacggtg gagtaagctt cccgccgatt
     2041 tttatcgaaa aatgcagatc atcaactatc aaccgcttgc tgagacacat gcctaaatag
     2101 aggttgtgaa atcagtgctt tgagctaata ccgaaggtca gctgattgaa cattgtttag
     2161 aagagattgt gacaactgcg atttgctttg agtaataaga acgctggaca gaaaatagct
     2221 tctcatattt tctagtctag tgggcttatt gccgagaagc agcagatgga acatcgttta
     2281 gaagagattg tgacaactgc gatttgtttt gagtaataag aacgctggac agaaaatagc
     2341 ttctcatatt ttctagtcta gtggacttat tgccgagaag cagcagatgg aacatcgttt
     2401 aaagaagaga tcggtacaaa ataaaaatat ctaaatttaa gttttatcta agtttcgctt
     2461 tgtacctttg attcaaatca aaggaggcga tcttgatgct gaaaaaatgg atcacactat
     2521 tttttgccac tccccattct agaatcaacc cgtatgatgt agataatgaa ttcacgttga
     2581 aaaaagattt ctcaaataaa taaaaaccat ccgtaaaatc atcaatacga ttttacggat
     2641 ggtttttgat aacttctttg aggataggct cttgctattt aagaatgtaa gaaacattat
     2701 tgttacatag tatgagagtt taatcctctg ttatttatgc tatttaaatg cttgattcac
     2761 atcttcgatc atcagtttca ttgattctaa accgcccccg cttaaatacc agacatctgg
     2821 ttcaagggag atgatctgct gatttttacc agcattcgtt tgtgctacca gttcattggc
     2881 agaaatatca tctttgctgt cgtctccacc aatagctttt gttctgtcta ctacaaataa
     2941 gatatccgga tttttttcca gcacatattc ataagaaaca ctttgaccgt gagtggatgc
     3001 ttcgatttga tcatctgctt gttcaaagcc aaataaatca tggatgaaac cgaatctaga
     3061 accagatccg taggctgata attgcccttc attcacaagt gtgactagtg cttttttgtc
     3121 agtagcttct gctttctttt tcgtctgatc gattgcacta gataattcct ctaattgagt
     3181 tttagcttct tcttgcttac caaagatctc ccctaaagca gtgacatttt gctggaaaga
     3241 ttcccatgga tttttcgcat ctaaagaaag gaaaatggtc ggagcaattt gacttagatc
     3301 tttttgaaaa tctctttgtc ggccagaaat gatgattaga tctggctgaa gctgattaat
     3361 tttttctaag tctggttctt ttattccacc tgctgattct acttttttat aggatgaaag
     3421 atactcagga aggctgcttg tagctgctcc aactacctta tctccaacac caagagcatc
     3481 catcgtatct aaagaaccat tgtcgaatac gacgactttt tctggattat ttggcacctc
     3541 gatttggtct ccattactat ctgttatcgt caatgtagtt tcagtttgtg tgctgctttt
     3601 ttttgtgtct gcctgactgg tgttgttgct atttccacat gctgctaaac caaatgatgc
     3661 tagaatcaag atacctacta ctaatttttt catctcttct ctcctaatgt tagttttaga
     3721 aataaagaca gaatctgcgg ccttcaatct cacaaatccg tatgtccatt tcgtataatt
     3781 catccaatac atcttttttt atcatttctt gggtcgttcc ttgcgtaaat aactgtccat
     3841 ctttcattgc aacaatttcg tctgcataac ttgcagcaaa gttgatatca tgaagaacaa
     3901 taacaattgt tttcccgatt tcttgtacta aattttttaa aatctgcatg atttgaaccg
     3961 caaaattcat atctagattg ttcaaaggtt catccagtaa aatataatcc gtgtcttgcg
     4021 ctagcaccat tgcaatatac gcacgttgga gttgaccgcc tgaaagagtg tcaatcgatt
     4081 gatccgctat ttcttctaac ccaatgtgtt ccagtgattc ctgaacgatt tcctcgtctt
     4141 ttgtcgtcaa ttttcctttc gtataaggaa atcgtccaaa ttctactaat tctttcacgg
     4201 tgatttggac atttatttca ttcgattgtc gtaggatcga taattttttg gctaattcat
     4261 tggatttcca agcctttact tcgttatggt ctaaatagat ttcacctgta tcttttggta
     4321 tcaaacgact gatcatcgac aacaccgttg actttccagc accatttgga ccgataaaag
     4381 cagtcaactt cttttcagga atcgttaact gtacatcttt aaccactgat tttccatggt
     4441 acagtttatt gaccttttgt aaatcaatca taggatactc ttcctttcgc gaatcaattt
     4501 tgtgacaaaa taaattcctc cgacaaattc aacgacgaca ctcactgttg tattccagcc
     4561 gaaaatctgc tctactgcga gctgtcctcc tgtcagaaac agcatagcta ccagaaagct
     4621 tccgataaat agctgaccat gacgataagt agaaaagaca cgataactga tatttgcgac
     4681 aataaaccct aaaaaagtaa ctggacccac aagagccgtt gatgtccctg tcaaaagact
     4741 gacacaacat aaaatcaaaa attcaaaacg atctgtgtct atcccgaggt ttactgcttg
     4801 ttcatttccg agatggaaaa cgtctagcac atgtcgatat cgaaataaca ggtaccagca
     4861 gccgataatg atcaaagaag caggcaaaag atactgactc tggatattgc caaagcttgc
     4921 aaataatctg ccttgaagca aatcatattc attcggatcc atgatgactt gtaaaaaggt
     4981 actgacacta ccaaaaagcg tacctaaaat catccctgac atcagtaata aaaaaagatt
     5041 cgctctcatt tttttcaaaa gaaagaaaaa taacaataga ctcagcccga ccatcagccc
     5101 aacgttcaat aagaaaaaaa ctgtacctgt ctgctgaagc atcgtcactc ctccgataaa
     5161 gaagaaaagg accgtttgga caaggacata taacgactca agtcccaata ctgaaggagt
     5221 caaaaactgg ttatgcgtaa ttgtctgaaa gctgattgtg gcactgctga cagctagcga
     5281 gacaaggata aatgcaaaaa ttttcttact cctcaatgca catgcaaaat gccagtttcc
     5341 atatgtatgg atcgtcaagt acattcctgt aacaaataaa acaagaacaa gcataccaat
     5401 aaaaatagat ggcttttttt taagcatatc gtcgtcccct tatcagaaga taaaggaaga
     5461 tagccccacc gatgattcca acgatcacac tgactggtat ttcataagga aaaatcaaca
     5521 cgcgagcgac aagatcgcaa aatactaaga acaaactacc tgctaaagca gttacgccaa
     5581 ttgttttgtg cacttgatct ccatatattc tagagacaag attcggaatg atcactccga
     5641 taaaaggaat gcttcctacc atgatcaaca tgactgcact agccaatgat actaagccaa
     5701 tgcctaaaaa ctgcatcttc tggtaatcta ttcccagatt tactgcaaac gattcgccca
     5761 gaccaatgat cgtaaatcgg taagcaaata gccaaaccaa gagcaacaat ggaacaacag
     5821 catacaataa ctcataattt cctcgtatca cagtcgcaaa attcccttga agccatgaag
     5881 tcatattttg taccaactgg aattgataag caaaaaaagt agcaactgaa ccaatgatat
     5941 tccctaacat gactccaatc aacggaagca tcaattgatt tttctggagg aacaatcgcg
     6001 taagggataa aaaaagcaat gttcctgcaa aagcaaagca aaatgctgtc aaggatcgga
     6061 caatcaagga tgcaccaggt aggaaaatca tagccactaa aatacctaaa cgcgcactat
     6121 ccatcgtgcc cgcagtcgtt ggtgaaacaa atttattttg ggtcaaatgt tgcatgatca
     6181 acccacaaat actactggtc gctcccacaa ggatcaaaca aatcgttcgt ggcaaacgag
     6241 tcactagcaa gatttgtaag ctttgcccat ctaactggag cagttctgac aattgtacat
     6301 tcgatactcc gatgaacaat gaaatactac ttaaaataac taataatgct actagtcgtc
     6361 cgtagcgcat ccaatccacc tttctaattg agaatgattt tcattcttat tttaagtgaa
     6421 tcgttgaaca aaagcaaccg ctttttcata tgttttaggt attattggga atatgctata
     6481 atcgtaagaa agacaaagag aagaaggagg gtatttttta tggcacaaga tgatgtaaaa
     6541 agccatttgg ataagggact ttatggtaca cctttagtga accctgaaga acagcataaa
     6601 tatttaggga cattcaggga acgctgctat cttagtatga ctgttgctga aatgatccaa
     6661 ccaaaaaaca aagaaaactt tttaaaagaa ctcaaaaaac atccagatgc ttccatactc
     6721 ttgaatgggg ctctacccct ttctgtccaa aaccaatata tccagctagc aacgaaaaac
     6781 aatagccgtt tcactgtagt caacgattac gtcactgcaa atcctgagga attcggattg
     6841 ctcttaacag caaaagaagc agtaaatgaa cctgtgattg atatcgaaaa aaaataccca
     6901 gaagaaaact cttcgccagc tgaaaaagaa aaaccaaaaa agaaaaaaag cttctgggat
     6961 cgtttatttt aaaacgaaaa aacacataaa tttttacatg caaaaaaagc ttttgatcag
     7021 attgacatca aataggacaa tctgtcaaaa gctttttctt ttaaaatact tcaggatata
     7081 acaatttcaa tacattcatc gtcaatcttc tgccttttcc tgcaaaagga taaacatgca
     7141 tatgcatggc tggattgaaa tttgcttcga tgatcccata tgctttttta tcagttgtag
     7201 gatcgatttc tttatctggt ataatcaaat cgataccact aatcttagct cctagtgctt
     7261 ctaccgcaga aacggcgatt tttttgtaag cttcagaaaa ttcatcagtc atatcgatcg
     7321 aatcaccacc tgtactgata tttgagttct cccttagata aacaatttga ttggcttggg
     7381 gaactgattc gatcgtcaac ccttgttctt tcaacatcaa ttgttctaat tcacctaatt
     7441 ggatcaattc taaaggtgca cgatgattcg ttccacgcaa agggtcacta tttttttctt
     7501 caacaagttc tttcactgtt cggatactgt caccaataac attagcaggg actcgcaaca
     7561 tgatggcttg gacttctccg tcgatcacaa agaaacgata ttccgttccc ggcataaatt
     7621 cttctactaa aaccgaactg tcttcacgaa aagcaatggc taaacctgct tgataatctt
     7681 ccagactagc tccttccttg aaaatcgtga tacctaaccc gtaattggtt gatttaggtt
     7741 tgatcacaaa tgcctgctcg gcaaatctcg gataagcttt taccgcttct tccatggatg
     7801 aaaattctgc gcctcctggt acccggaacc cagcttcttt taagactttt ttcgttactg
     7861 ttttgttttc catgataagt gggacgatat agctgtcctt acttgtcatg tttgcatttt
     7921 tcacatattc tacatggtct tggaattgca gacgcaaaaa ttgatccgac tcatctaata
     7981 ctttgacttg gacacctttt tggatggcat cgaacataaa tatctgagta gaaagttcca
     8041 tttcgcgaaa accggccaac tgatagggac gttcatggct ttctttataa tattgatgtc
     8101 ccaatgccac agcaacttct tgctgactcg atagctgtgc ttcttgatag agtttcgctg
     8161 ctaacgtttg ttcaggatga tccatttgag tcaatgcttc ctctactaaa taaagagaat
     8221 ccaaccaatc caattcaacc agcatttgct tcattccttc aagaatttct ctaccttctt
     8281 gatgaaattc tgtttgatct gatggttgtt ctagtgctac tttgttattt cgagtcgtac
     8341 cttctgctac ccattggtca cagtcttctt tttcttctgt ccataacatg tacatcagga
     8401 ataattgtaa gaaccgaact tgctctggat tgatacccaa tcttgcataa ggattcaaat
     8461 cgatattccg caattcaata taacgaacac ctgttgttgc taaatctgcg actttctttc
     8521 cgcctcggaa acgaagcggt gtataaaatt ctttctcctc cgaaagtttc ccttcttcga
     8581 tcattcgctc gatatctgct aaatactgtt tcatagaagc ataggaaact ttcacatttg
     8641 gatgattcgt atagcccagc gcactgttgc gaatactgcg aacaggttct tgcggatgtg
     8701 attcatctaa aaagtagttc ttctcactca ttggtgaagc accgaataaa taagtgatca
     8761 tccagcgata gcgcataaag tttctcgctg ttttcaaata aagttctgtt ttgaattcgg
     8821 aaaaatcttg aaattcttcc tgatggctga acaatgttct tagcaactca tcgccaaatt
     8881 caaaattgaa atgaataccg cttaccatct gttttcgctt cccgtattct tttgctaaat
     8941 aacgtcgata caagacatct tcgaaatttt ttaattttgc aataatgatc tcttcgtctt
     9001 tttccggtaa ggcaggtggc atacttaatg gccagatcat ctcttctttc ggtatagaac
     9061 gagcagtcac atcataaaca gcagcgagat actgaaataa ttcgggaata gaatcggtaa
     9121 caggggtgat catctctgtt tgtgtttcgc taaaatctgt ttggatatag gggtgatagg
     9181 atcgatcgcc aaagattgct ggatgatcgg tttttgctag atttcctgca agatcaaccc
     9241 gctgtccttc ccgttctata ccaaatcgag cttggtcgat aaaagggcgc gcattgacat
     9301 gcaataataa ttgcttaaaa ttcatcatta agtgacctcc aattcatatg aatctaatta
     9361 taatcaaaaa gtctaagaaa aagaacgaaa ttgtttgaga accgcttaca aataagtata
     9421 aaaatgaagg catcaaaaac acagctatgt taaaaacaat cattcaaaca aggagctgca
     9481 acttttgtca cagctcctgc tttttagaca tgggttattc ttcttttagt aatcctttga
     9541 acaatccaac gacaaaatca tttggatcaa acggctccaa gtcatcgatt ccttcaccaa
     9601 gaccgaccaa tttcactggt aaatgcaatt cattacggat agcaagtacg ataccgccct
     9661 ttgctgtacc atctaatttt gtcaaaacaa gaccggtcac atctgttgtt tctttgaatt
     9721 gttttgcttg aaccatcgcg ttttgaccag tggttgcatc taatacaaga agtgtttctt
     9781 ggggtgcttc tggtaattcc cgctggatta ctcgttttat tttttctaat tcgttcatca
     9841 agttcacttt attttgcagt cggcctgcag tatcaactag taaaacatct gcctgctctg
     9901 tctttgctct ttccaaagca tcgaatacga cagctgctgg atcgcctcct gcattgccac
     9961 gaacaacttc aacaccagcg cgttcgcccc agacaactaa ctggtcgatt gctccagcgc
    10021 ggaaggtatc tgctgctgcc atcaatactt ttttaccttc gtttttgaac tgatgggcaa
    10081 gtttaccaat acttgttgtt ttccctacac cattaacacc gacaaacaaa ataactgtta
    10141 gtccgtcttc ttggatattt agttcattta cttcattgat tccttccgct tcatagagat
    10201 ccacaagttt ctcgatgatc gcattttgga cttctgccga ttttttcgca tttctgagtt
    10261 tcacttcttg acgtaatgct tcggtgattc gtatcgctgt ttcaaaacct acatctgcac
    10321 cgataagtgt ttcttctaat tcttcaaaaa agtcttcatc gactgttcga aaattggcaa
    10381 acaactcatt catccgttca ccaaatgttt tacgcgtctt ttctaaaccc ttgtcgtact
    10441 tctcctgtac ttcaacttct tctgttttct cttcgccagt aaaagcacgc ttgattttat
    10501 caaaaaatcc catttgactc cccctactct aatacaaatt tttggatcac tttggctacg
    10561 ccattttctt cattgctggc tgtcacgaaa tctgctgctt ctttgacgaa aggcacagca
    10621 ttctccatcg ctacgcctag tccagcatat tggatcatcg gtaaatcatt ttcctcatca
    10681 ccgatcgcca tgatttcttc ttgtttcaaa ccaagatctt ttgccaataa agaaatacca
    10741 taagcttttg taacaccttt tggcataaat tccaaaagca tacttcttgt tttgatgatc
    10801 tcatacattt catggaattc tgctggtatt tctttgattt taggatctaa ttcttcttgg
    10861 tcataagcga ttactgcctt attaagcaca aattcatctg taatatcgct tatatccgct
    10921 ggttggaatt gcagcagctt attcaacaca ttgtacaagg attgcttcct tggggcacta
    10981 ggcagctgta aaaccacatt gtcagacaaa acatctagtg gcagatccaa ttgctgagcc
    11041 agtttgtata agcgttggat atctgatact tccatcactt tcttttccat gatttcacca
    11101 gtatcatttt tttgtaccaa accgccgttg aaagtgatgc tgtagtcacc ttcatctacc
    11161 agtcctaatt cttgcagata atgcgccatc gcagctaagg ggcgtcctgt acatagaacg
    11221 atcttgaccc cttgctgttt tgcttgtgct aatgcttgtt tattctcatc tgaaatcagc
    11281 ttttgttcat ttaataatgt accgtccaaa tcgattgcta ctaatttgat cattttcgtt
    11341 cctacacttt cgtttcgttg attttcgcct cttcattgat atcttccatc cgtacagaaa
    11401 cgatttttga tacaccggat tcctgcatcg tcactccgta cagtacatct gctgcgacca
    11461 tcgttccttt gcggtgtgtg actacgataa attgtgtgtc atcttggaaa tcgctcaagt
    11521 aacgaccaaa acgtttcacg ttggcttcat ctaacgctgc ttctacctca tctaagatac
    11581 aaaacggaac aggacagaca cggataatgg aaaagagcaa cgcgatggct gtaagtgctc
    11641 gttctcctcc agaaagcaaa cttagacttt gcagtttttt gccaggaggt tgtacttcaa
    11701 tctcaatacc tgttttcagc aaatcagttg gttccgtcag tactaattca gctttaccgc
    11761 cgccaaacat atttggaaag acaattttga attcttgacg gatagcttca aatacttctt
    11821 tgaatcttgt ccgcacttca tcatccatct catccatcgt ttcaaacaat tggtttttcg
    11881 ctgctaacag atcatctcgc tgtgttgcta agaatgtatg tctttcactg acttgttcgt
    11941 attgttcgat tgaatttaag tttactggtc ccagtttttc aatctgttct tttaaggatg
    12001 aaacctttgt tctactgtct tcgatatcgg ttgtttcttg atagtcttgg gaagctttct
    12061 caaatgtcag ctgatattct gactgcagat aggtcaaatg attatctaaa atcatttcag
    12121 ctcggtcttt ttggacttct aatttggatt gttccgacag tcgtgctttt tgctcacgat
    12181 tacgctctgc caagactgct tcaagcttat cgatctcctt ttgccttcta tctcgctgct
    12241 ctttggcttt tgccaactcg gcttttaatg tttctcgctg agcagataat tcgttaattt
    12301 gtttttccaa actttcttct gtgacttcat gatcagaaaa gtctgctgtc aatgtcgcca
    12361 actgcttttc tatcgcttct tgacgttcgg ttgcctctgc tttttggact cgggcaccgc
    12421 ggagctggat ctgcagatga ttaaactgct ctttcaagac agcaagatct gcttgttctt
    12481 gtgctttttg actttgtact tgtgctcttc tggcttccat ctggtcgctt tcttgactta
    12541 acgatttgat ttcttcgtcg attttttgtc gttgcgattc aagatctgtt tgtttgtctt
    12601 tcaattcgtt cgtttgcttt tcgtaatctt cgataaactg ctgcagttca cgtgtctcaa
    12661 aagttgagat ttgcttttct ttttcaaaac gttcaagttc atttatgatg ttttgtagct
    12721 gatttgtcgc ttcttgttct tcaaaacgta attgttcgcc tcgtgtacgc aaaacttctt
    12781 gttcttcggc taaacgagct gtttcttgtt gcagctcctg aacttttttt tcttgtgctt
    12841 ggagttgttt atctgcttct tcaaattcac tagtcaactg tttcaattct tgattttgta
    12901 caaacaaact gccttggttt cctcgtttat tcgctccacc tgtcatggat cctccggcat
    12961 tcatcacatc accttctaag gaaaccactc gatattgata gcggatcgtt tgcgcgattg
    13021 cattggcact agttaagtct ttggctaaaa ggatcgttcc tagcaggttt tgaaccacag
    13081 tttggatttg atcgggataa ctgacttgtt cactggcaat ccctataaac ccttctacag
    13141 ctgctgcttg tgtcaatata tgagccggta attgtcttgg tttgattgtc gtcagtggaa
    13201 ggaatgttgc tcttcctcct ctttgctgtt tcaaatacgt gatggcttgc cgagcatctt
    13261 tttcattttc aacgatgaca tgctgggctg ccccacctaa agctgcttcg atagctaacg
    13321 tgaaatctgc aggaacatcg atcagttcag ccactgctcc gacaataccc gaaagctgct
    13381 gtttatgctg caagaccaat cgaacccctt ggtaaaaacc aaaatagttt tcttggattt
    13441 cttgaaggct tttcttacga gcacgtagct gctggacttg attcattaat tgatacattt
    13501 taggctgttc ttttgctaat gcttcttgga actttttccc gttttgttgg atcatttcta
    13561 acttctcttt tgtttcagtt aaagatgatt ttagctgcgc ctgcttttgt gtcaattctt
    13621 ctttttgtaa gatcaatcgg tctactgatg cttctacttc cgattgcttg gctaacgtct
    13681 gcttgctttt tgctgtttct tgtatatatt gacgttctag atacttcagt tcgtttccta
    13741 cagctgcttg ttcttgcatc aaatcaacat attgatctct taattctgcc aataattctt
    13801 tcgttgattt ttggtatttt tctaattctt gctgtgtttt tatgagattt gcttcagctt
    13861 tttggatctc tgtttctttt tctgctgctg ctttcatcaa gctttcttga agcttttcaa
    13921 aatgtttaac ctttttttgt gcttctgcta atgaagtttg atattcttgc gagctttttt
    13981 gtgtatgctt tgtccgctcc tgcaaaacat ctttttgtcc ttctgtctgc ttcagttttt
    14041 ccgaaagatc taataatact tgctgatttt tttcgatcag acgatcggct tgcgcatttt
    14101 ctttacgctg cttcgccaaa atggattctt gttcttggat cgactcagat agtttgccta
    14161 attctagatt gaatttcgca agctgtgctt gtttgttgtc ccaatctttt tttgctgtct
    14221 tgatttcagc aaccatcaaa gagacatcgg tttgagtcag tgtttctttt agccgtaaaa
    14281 actcctttgc tgcctcactc tgggcggcca aaggagttaa ctgttcttcc aattcgtgga
    14341 tgatgtcttg gacgcggctg aggttgtctt ctgtttcaaa aagcttctgt tctgctttct
    14401 ttttccgctg cttgtatttc aagacaccag ctgcttcttc aaagatccca cgacggtctt
    14461 ccggtttact actgaaaatc gcttctactt ttccttgaga aatgattgag aacgattctt
    14521 ttcctaatcc agaatctaag aacaattctt ggatatcttt taatctgcag ctttgtttat
    14581 tgatgaaaaa ctcgctttcc ccagtccgtc gataacggcg ggttacactg atctcattgt
    14641 actccaatgg cagataatga tcactattgt ctaaaatcac tgtgacttct gcaatattca
    14701 gctgcttacg ggtatcggaa ccggcaaaga tgatatccgg catttttccg cctcgcagac
    14761 ttttagcaga ttgttctcct aatacccagc ggatagcctc tgtaatattg ctttttccgc
    14821 tgccatttgg tccaaccact gctgtgacgc tattttcaaa atcaatgact gtcttgtctg
    14881 caaatgattt aaaaccggct atttcaattc gtttcaaata caaagctttc cctcacttac
    14941 ttttacagtg ctgccaatgc attttccgcc gcagcttgtt ctgctaattt tttcgatttc
    15001 ccttgtccag tgccaatcaa ttgatcatct acataaactt cgatccaaaa tactcgttca
    15061 tgagctggac cttcttcatt gatcaatcgg tattcgattg atacatcacc tgatttttgc
    15121 aagacttctt gtagttttgt tttatgatcc atctcatgtg aaaaagcacc tgctttgact
    15181 ttaggaaaaa tcactttttc gatgaatgca tgggtcgtgt cgaatccctg gtcaagatat
    15241 aacgcgccta aaaaagcttc gaataggtcg caaagcaatg ctggtcgcgt acgtcctcct
    15301 gaattctctt ctcccttgcc caacaaaaca tattgatcaa agtgacattc tttggcaaat
    15361 ttcgacaagc tgtcttcgcg aacgatcgca gctcgtgttt ttgtcaattt tccttctggc
    15421 aaatgaggat ataaacggaa taaatactga gaaaccatca gttctaaaac ggcatctcct
    15481 aaaaattcaa gtcgctcatt atccgataat tgcagattgc gatgctcatt cacataggat
    15541 gaatgggtaa aagcttgttc aagcaaattt agatcgtgaa aaacgatgtt atagttttct
    15601 tttagttctt ttgtcagctg gttgtccatt ttccacattc cttttatgga tattttttcg
    15661 atacttcctt attatactgg ttttttgtca aatttgaaat ggttcggtca aaaggtaagt
    15721 aaagctttaa taatcctagt gattgacgag aagttgtgaa aaattcgttt agtttcgagt
    15781 atgaagaaaa aattttgtga aaatggcttt ccatattttt gcataaattt gacttaatac
    15841 cgagaaactg gactttgaac accgtttatt ggagaagtca tgaaatcagc gccttgacct
    15901 gaatattaga gaaataaaca gaaaaatatc aaaaaaccga aaaaggacat cgcccttttt
    15961 cggtcatttt tagtgattat gaacgtgaat ctgctgtaac agaaacattt gcccatgggt
    16021 ttggtacatc ataagaccaa tcccagtctt tgacacggtc gttgactggc aatacttcat
    16081 tacggaacaa cgttggaatc gcaaatgctt cttcaaatgc atattgttgc catttttgat
    16141 atgcttcttt ttggtactct tcatcaaatg ctttgcttga actgatatcg ttgatcaatt
    16201 ttgtattttc ttctgattca taacgagtgt agttgaacgc agcatttggt ccccatagtc
    16261 cacttggatt tggatcagtt cctgtattcc aagcagcttg gtaaacatca atttcaggat
    16321 catcgttttc taatttatca tagaaagaat tgaattcgat cagacgacct gttgtgtatt
    16381 ggacatccaa accaatttct ttccattgtt ggatatagta atctgccaat ggttgcgctg
    16441 tttccccacc ggacatcgaa gcaaagttga ttgttagttt atttccatct ttgtcttctc
    16501 ttaaaccatc gtcatccaca tctttgtagc cagcatcatc taatagtttt ttcgctttgt
    16561 ctagatcttg tgtgaatcct ttcatctcat catcatggta tgactggaat actggaggaa
    16621 tcaatgaagt cgcattgctt ctcaaacctg catagaaacg ttcacctact gcattattat
    16681 ctaatgcata gcccattgct tgacgtaatg atttatcagc catttttgaa tctggattgt
    16741 attccacact ctttgtatct gcattccatt tacctaactt gaatcctaga taagtataag
    16801 aagtttgttc acggcctaag tttgtatagc cagcaatttc tttataggta tcgtaagtat
    16861 ctgttggcat ttggaaaatc atatcatata atttagagtt caatgcttca gttgcacttg
    16921 ccatcggtac agatttcatc gtgatctttt tcaatttagg cgtaccattc cagtagtgct
    16981 cgtttggcaa gtattctact gattctcctg caacgatatt actcatgtaa taagggccaa
    17041 aagtcactgg atttttacgt acttgatcgc ttgattccat atctttgatt gctacacctt
    17101 ctaacgtatg ttttggtgct gcatatgacc agataccacc acccgattga agcatgcttg
    17161 ggtttacttc tttatattcg atttcaactg ttttgtcgtc tatttttttg attccggaga
    17221 ttgtgtcggc acttccatca tgatattctt ccatacctac gatgttacgg aaagtgtcgt
    17281 cataacggat accagtgtaa tctttattcc caatgatttc gtaagggaaa atcacatcgt
    17341 ccgccgttac atcttcacca tctgaccatt tgacgttgtc ttttactttg attgtcgctt
    17401 ttttcgtatc ttcatctaga tcaagcgttg cagctccgtc gtctgtgatt ctgaaatcgt
    17461 catcgtttgc aaatagcggt tcatgagaag gagccataaa agctgcatca tatgaatctt
    17521 ggtaaaactc ccactggaac aatcctttaa actgactgtc cgttgcgacc gcagcttcta
    17581 attcgccgtc ttcgattgca tcatcattat tttttgttgc aattggaaaa gttaattcct
    17641 ctttgttatt gccgttgcct ccagagttgt ttccagaggt atctgtacct ccgcccccgc
    17701 atgctgcaag aacgacagca gacatcagcg ctgcaaatcc aaaaatcgtt ttcttcattc
    17761 ctatttcccc cttcgcttct atactagcct aaccgttggc gcgcatctgc agaacgcttg
    17821 aatgcttgtc caacataatt gatactcaac atcaaaacta aaataaggat tgatgcgggt
    17881 aaccagatcc attgccgatt cactaagact tctccgttac ttgcataacc gatcaatgta
    17941 cctaaactag gtacatttgc cggcaagccg aaacctaaga aagtcaaagt tgtctcgata
    18001 ccgatgtttg ccgcaaagtt catcgtcaaa ttagtaatga ttaatgaact tagattcggc
    18061 atgatttcgc ggaacatgat tttaaaatca ttggttccca ttgtttttga ggcactgaca
    18121 taatctcttc ttgcttcaga taatgtcttg cttcggaata atcgggcttt agctacccaa
    18181 taaaatgcac tcatgatgaa tataaaggac cagacattgt atctggaaat aatcgatacg
    18241 aagacgataa tgatcagcat gatcggtaga atcatgataa aatcaacgat ccgcatcaag
    18301 atgttgtcaa tgatccctcc gtagtagcca gagatgatcc ctaatccaac cccgatgatc
    18361 gaagtaagga tcgtgatcgc aaatccgatc gtaacagagt ttctagcacc tatgatcaat
    18421 tgtcctaata catcacgtcc gccttcatct gctcccagaa tgaacttttg tccttgtgtc
    18481 gaaactgttc ctggtgcagc gtatttgtcg aagatactaa catacatcac tttgtcttga
    18541 tcggtcagca tcgcaccgat aaagatcact aataataaaa ccactagcaa acctaatgaa
    18601 aaaatagcaa ttttatcttt tttaaattct cgagcaatca ttcgtatccc cataggagga
    18661 atactttctt gaactgctgt attgtttttt tcactcatcg atccattctc ctttctgatt
    18721 ggttttattg aatccgaata cgcgggtcaa cgatactcat gatgatatct gaaagtaatg
    18781 tccccactag cgtcgcgatt cctaaaatca atactaagga tgtgatcacg gtatagtccc
    18841 gttggccgat ggaatcaata aatagttttc cgactcccgg ataaccaaaa atcttttcga
    18901 taactacaga accagcaatc aacgaagtga tttcataccc catttgagaa gccatcggca
    18961 acgctgcatt tcggaaaata tgtcgactga atactttatt cgtcggtacc cctttggaac
    19021 gggctgttct gacaaaatcg agcgatttcg aatcgattac ttcacttcgc agatattgga
    19081 ttgtgacggc agtcgccaaa aatgcttggg taattgatgg taacagaata tatttcaaac
    19141 ggtctatcca ttgagtgata aatccacctt gcaaacctag tgtttgtgat ccacttgttg
    19201 ggaaccagcc taaacgataa ccaaaaacaa atagcatgat caagccgaag ataaataaag
    19261 gaacagcaaa actaaagaag ttgtagatta cgacaatctt atccaagatt gaattttgat
    19321 accggccagc aagcatcccc agaggcacag caatcaaata agtaaggaca actgtcaaga
    19381 tcgatagata taaagtgttg atgatccgtt cgccgatcaa gtcagcaaca gagcgtttgt
    19441 ataagaaact ttgaccaaaa tcgccttgca aagcatttcc gatccaacgg aagtactgga
    19501 cataccatgg gtcattcaag ccagctgctt gtcgcatctg ttcgatcact gccggatcct
    19561 gatttggatt gatcaaaccg gtaaatggat cccccggcat cgcttgggct aataagaaga
    19621 tcagaacact taaaataata acttgcggga tcatcaataa aacacgtcgc agaatcgttt
    19681 tccacattag cttgcacctc catctttcaa cgccactttg tgtgtagttg tgatcgtacg
    19741 caaatcgtag actcgaccag tcgcatcata gtattctttt tggttttgga tatactcttg
    19801 ttctactctt cgtcgatttt ctttgtggct ttccctattt tctacatcga tttgcggaat
    19861 agctgaaagc agacgtttcg tatagatatg tcgcgggtca ttataaatgt cttctcttgt
    19921 accgatttct acaaatcgac ctttgtacat aattgcgata ttgtcgcaca tgtgttttac
    19981 tacacctaaa tcatgtgaaa tgaataaata gctgaggccg tattcttgct ggattcgttt
    20041 catgaaattc aatacttgcg cttgaacaga tagatccaga gcagatacag gttcatcggc
    20101 tacgatcagt tttggattgg tagcaacagc acgggctact cctagacgtt gacgttgtcc
    20161 gcctgaaaat tcatgaggat atttatatag cgcatcttcc ggcatcccaa cgatatctag
    20221 cagtcctttt acctttttct tttcttcttg atcgcttaac cgttcaaaat ttcggatggg
    20281 ttcagcaatg atatccaaca ctcgcttttt aggattcaaa ctggacattg aatcttggaa
    20341 gatcatctgc acatccttgt tataacccat ttttttgcgg ttgcttcgct tcgttacatc
    20401 ttttccttca tacatgattt gtccggctgt taccttttct aatcccacaa cggcttttcc
    20461 cgtagtagat tttccagagc cagactctcc cactaaacca tatgtctttc ctttttcgat
    20521 aataaaatcc acaccatcta cagcatatac atgatccgtt actcgattga agaatccact
    20581 gcgaataggg taatgaactt tcaggtcttt gattgtgatt aattctgtca tctacacctc
    20641 tcctttttcg tctctaaagt ggaaatgctt atagcaggag caacgtacca agtggttcgg
    20701 tgcgacttca tgaaggatag gctcttcttc atgatcgctt gctggtatcc atggaatacg
    20761 tggtgcaaac cgacaacctt tacgaggcat gtttttcaga gaaggaacga caccttcgat
    20821 cacatggagt tctgcatcat gatttccttc ttgtggtata gagttcagta aagagcgtgt
    20881 ataaggatgc ttaggatgat caaacagttc ttttacacct gctacttcaa cgaactgtcc
    20941 gccatacatc actgccactc gatcagccat ttctgctacg actcctaagt catgagtgat
    21001 caagatgatc cctgtttttg tttcttcctg aaggtctttc agtaaatcaa gaatctgtgc
    21061 ttggatggtt acatctaggg ctgtcgtcgg ttcatctgca atgacgattg gaggtttgca
    21121 ggcgattgcg attgcgatga tgacacgttg tctcataccg cctgatagtt catggggata
    21181 ttggcgtcca actctcgcag gattaggaat ccctacttgt tctagcaatt ctagtgcacg
    21241 agcttgacgc tgttctgcat tcatctttgt gtgataggaa agtccttctt tgatctggtc
    21301 ttcgattctc atgagaggat ttaatgcaga caacggatct tggaaaatca ttccgatatc
    21361 atttccgcgg attttattat agagcgtttc gttcaggtcg attaaattca gatcgttata
    21421 taaaatctca cctgtaattt ttgtgttatt aggatcaagt aatcccataa tcgtagtagc
    21481 caaggtactt ttcccgcacc cagattcccc aacgattgct aaaatctcgt ttttgtctaa
    21541 agtaaatgaa acgtcatcta ctgcatcgta atagtcatct ttgagtcgga atcctgtatg
    21601 taggttctga acttctaata attggttatc ggacaatcga atacccctct ctgcttccta
    21661 aatggaagtt tttcttaata aaaaaggaca ttacacagaa taaatcgttt t
//
LOCUS       DAZKNH010000046        20805 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000046 DAZKNH010000000
VERSION     DAZKNH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.046"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(565..1590)
                     /locus_tag="ACG8MG_001552"
     CDS             complement(565..1590)
                     /locus_tag="ACG8MG_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0375599.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1865..3550
                     /locus_tag="ACG8MG_001553"
     CDS             1865..3550
                     /locus_tag="ACG8MG_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0375600.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3564..4847
                     /locus_tag="ACG8MG_001554"
     CDS             3564..4847
                     /locus_tag="ACG8MG_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0375601.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4917..5786
                     /locus_tag="ACG8MG_001555"
     CDS             4917..5786
                     /locus_tag="ACG8MG_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0375602.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5783..6619
                     /locus_tag="ACG8MG_001556"
     CDS             5783..6619
                     /locus_tag="ACG8MG_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0375603.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6645..7712
                     /locus_tag="ACG8MG_001557"
     CDS             6645..7712
                     /locus_tag="ACG8MG_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFQ0375604.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7709..8536
                     /locus_tag="ACG8MG_001558"
     CDS             7709..8536
                     /locus_tag="ACG8MG_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0375605.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8581..9606
                     /locus_tag="ACG8MG_001559"
     CDS             8581..9606
                     /locus_tag="ACG8MG_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0375606.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9603..11786
                     /locus_tag="ACG8MG_001560"
     CDS             9603..11786
                     /locus_tag="ACG8MG_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFQ0375607.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11786..12916
                     /locus_tag="ACG8MG_001561"
     CDS             11786..12916
                     /locus_tag="ACG8MG_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0375608.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12913..13590
                     /gene="pgmB"
                     /locus_tag="ACG8MG_001562"
     CDS             12913..13590
                     /gene="pgmB"
                     /locus_tag="ACG8MG_001562"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0375609.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13674..14201
                     /gene="msrA"
                     /locus_tag="ACG8MG_001563"
     CDS             13674..14201
                     /gene="msrA"
                     /locus_tag="ACG8MG_001563"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0375610.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14402..15088)
                     /locus_tag="ACG8MG_001564"
     CDS             complement(14402..15088)
                     /locus_tag="ACG8MG_001564"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFQ0375611.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15168..16706
                     /locus_tag="ACG8MG_001565"
     CDS             15168..16706
                     /locus_tag="ACG8MG_001565"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFQ0375612.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16826..17293
                     /locus_tag="ACG8MG_001566"
     CDS             16826..17293
                     /locus_tag="ACG8MG_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFQ0375613.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17827..18255
                     /gene="rplM"
                     /locus_tag="ACG8MG_001567"
     CDS             17827..18255
                     /gene="rplM"
                     /locus_tag="ACG8MG_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFQ0375614.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18271..18663
                     /gene="rpsI"
                     /locus_tag="ACG8MG_001568"
     CDS             18271..18663
                     /gene="rpsI"
                     /locus_tag="ACG8MG_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0375615.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18751..19977)
                     /locus_tag="ACG8MG_001569"
     CDS             complement(18751..19977)
                     /locus_tag="ACG8MG_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0375616.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(20049..20339)
                     /locus_tag="ACG8MG_001570"
     CDS             complement(20049..20339)
                     /locus_tag="ACG8MG_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0375617.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNT"
BASE COUNT         6717 a         3741 c         4322 g         6025 t
ORIGIN      
        1 ttgtcacaac ctcttttttg gtttgtctaa aataatttgt ctaactagaa gaaaagtggg
       61 aaaggatctt aacaacatcg gttcgtatcc aattaaggta tcggaacgat catcttccaa
      121 gagaagtcaa tcaacaaaag taaagataga tcattttttg aatgcaaaat aaattgatgg
      181 gaagatagag cggttatttt taatattttg ctagctatta ttgaataatt cctcgaataa
      241 aacaaaacca gtagacatgt ctgttattct atttatgttt tagagataag tatccccaaa
      301 taacatggat tgaaataagg gtaaatgtgt ttaaaaaaac gaactataaa aacttataca
      361 caacgtggtt aaaaatagtt ttttcttccc tgtggaaaaa atagtttaac acaaacatta
      421 tagttatcca cagctatcta aaacagatgg aaaataagaa aaattcgcta aaataccggg
      481 agggggtaac gagggatgag tgattgaaaa aagaacagag taactgactt atcctcagtg
      541 tttctgttct tttataattc tgttctacat ttcttgttgt tggatcgaat ctgtgagagt
      601 aatctcggtt gaaagaatga ttcttttttc ttcagggtat tggtggtgga ttagttggaa
      661 taaaagttct gtggctttga ttcctaactg atttgtcgga atgtctatcc gagtcaactg
      721 tggcgaaaat agggaggaca ataagggatc actgaatcct gcgatcgaaa gatcatcagg
      781 tatctgcttt ccagattgtt ctgccacttt gtaaatactc aatgatttta atgcatcggt
      841 agaaaaaatc gcagttggtc tttctcgtag cttcattaaa tcattcattg ctgccatgct
      901 atcttctaag cgaaagccgc cgtctataat ccattcatta cggatatcta ggtggtgatc
      961 aaataagcat tgacggtaac cattgagacg atcattggat acataaactt ctgtcggagc
     1021 atggagacag ccgatatgac ggtgtccatt cttgatcagt gtttccacca aatcgtaact
     1081 atcctggaaa ttatccgtgt ccactgagta gatgttcgtg tagtcgtttc cgacttttcc
     1141 gattaccaca ataggaatat tgtattgatc caattcaaaa agaagtgttt catccatcgt
     1201 agagcttaaa ataataattc ctcggatcat tttttcttgg atctttttcg cgcatttttc
     1261 tagctcttct tctggtgttt ttgccgtttg tagaatcaag tcgaagttat tttgttcaga
     1321 aacggcagaa atggattgaa taacatctga aaaaaaagga ttgccatagg tcgtatgaga
     1381 agtacgatga gataatgcta gaatcgcgtc aaaaccattt cctgacagtc ctcttgcaat
     1441 tttgctcggt tcataattca attctttgat tgcttgaagt acacttgctt ttgctttatc
     1501 agagatattt ggctggttat ttaatactct ggagacagtt gattttgaga cattagcttt
     1561 tttcgcaatg tcataaatag taggtttcaa ttttatatgc acctcctttt tgggagcgtt
     1621 ttagaataat tccatagtac aatagacatt cttttttgtc tatgattttg aaaatttagg
     1681 aatagcttgc atgatcatga gagaccatat ttttttaaga agctattaat tattgataca
     1741 taagctttct tatttgtatt tttgtgtata tcaaaattat ttaaaaaaat gctattgaca
     1801 gtaagcgttt tccataatat gatcaaatcg ttgggaacgc tcccaaaaaa gaaaggggaa
     1861 tccgatgtat acaaaacttt tacaagcgat ttatcgcgac tcagatttgt ctgagaaaac
     1921 cgcctctatg attgaacaaa aagtcagtgt ttatcagcag aagaaaggcc aggcaggcaa
     1981 actgcattgg gacgaagaag atattttctt gattacttat ggggatcaat tttttgaaga
     2041 gcagcaacca actttgcgta catttaagaa attttatcaa aagtatttgg cagatacatt
     2101 tagagttgta catttcttgc catttttccc atatacatct gatgatggtt tctccgttgt
     2161 aaattatcag gcaatcaacg accgtttggg cacatgggaa gatatagaag aaatgagaag
     2221 cgaaacacgg ttaatgttcg actttgtctg caatcatatg tctgccgaaa gtacttggtt
     2281 tcaggaatat ttaaacttga atgatacgta caaagatttt tttattgatt tgccactgga
     2341 tacagataca agcagcgtga ctagaccaag gaccagcccg ttacttacaa cgttcattga
     2401 caaaaacggg aaagaaaaac acgtatggac gacttttagc gaagatcaag tagacttgaa
     2461 ttttaaaaat ccagaagttt tattgaaaat gatcgatatt cttttgtttt acttagaaaa
     2521 aggcgcttca tttattcgct tggacgctgt gggattttta tggaaagaac taggaacttc
     2581 ttgtatacac ctgccagaaa cgcataaaat catccaatta ttccgtgcaa tcgctgatga
     2641 agcagctcca ggaacggtat tgatcactga gacaaatgtg ccacacaaag ataatatcag
     2701 ttattttggg aatggcgaga atgaagcaca gatggtctat cagttttcgc taccaccttt
     2761 gacccttcaa gcgattcgtt cgggtaatac aagtaagttg aaagaatggt taaaaaccat
     2821 cgatttcgca tcgaaaaaaa caactttttt taatttttta gcctcccatg acggtatcgg
     2881 attgaatccg gtacgaggta ttctgccgga aaaaacgatc cttgaattag tggaacagct
     2941 gaagaaagag ggtacccgag taaactacaa gaaaaaccca gatggaacga ccagtccata
     3001 tgagataaac gctacgtatc tagatgcgtt aagttctcca acagatacga cggaatggag
     3061 aagcaaacgc tttttagctg ctcattcatt tttggtaacg ttaccaggcg tgccggcaat
     3121 ttatattcaa agtgtgctgg gaagtcgaaa tgattacaaa ggcgtagaaa aaacaggaga
     3181 gaatcgaaaa atcaatcggc aaaaattttt gttagcagat attgagaaag agttgcagac
     3241 agatccatta agaacagcag tatatggtga attgacccag atgctatcta ttcgaaaaaa
     3301 agaaaaagcc ttccatccga atagtttgat ttacttactg gactcagcag attcttttat
     3361 cagttttatt cgcgaagcga aggaagagaa agtcttagtt atccataatt tttccaatca
     3421 atcagcaggt tatcttttac caaaaggaga ttacctagac ctgttttctg gacaaattat
     3481 ccattgcagt caagaacgaa agttgatatt ccagccgtat gaatatatgt ggctgaaaat
     3541 aataaaataa gaggtggaaa aaaatgaaaa aaattattgt tactacctgt ctgctagcta
     3601 ctggatttct tcttactgct tgtggtgaaa gtcaaagctc taacgaatca caacagacaa
     3661 cgattcaatt catgcactct tctgtcgaac aggaacgatt aagtgtgatc aatgaactgg
     3721 ttgctgattt tgaaaaagaa aatccagaca tcaaaatcaa gcaagtgccg gtagaagaat
     3781 cagcgttcaa tacaaaaatc gttacgttag ctcgttccgg taaattacca gaagtaatgg
     3841 aagtcagtca ggattttgct aaagtgatgg ataaagatga actgattgat cagacggcag
     3901 tccaaaacgt tatcgaggaa gctggagaag ataattatta tgacggagcc aaaaaccttg
     3961 ttcgttcaga agatggaaaa agctatattg ctgcaccaat tagcggctgg gttcaaggta
     4021 tttggtatga caaagaaaaa ttatcagaag ctggtttttc agaaccagaa aattgggatg
     4081 atattttaaa aattgctcaa catttcacag ataaagagaa taaacaatat ggaattgcta
     4141 tgcctactgc tgaaggaaca atgagtgagc aagcgttctc tcaatttgct ttgtcaaatg
     4201 gagcaaatgt tttggatgat gaaggcgagg taacgatcga tacggaaaag atgagagaag
     4261 cattgtcgtt ttatcaaaac ttatcccaat acacgatgcc aggctctaat gatgtgacag
     4321 aaatcaaaga tgctttcatg aatggaacag caccaatggc aatctattca acttacattt
     4381 tgccttctgt ttatgaagcg gggaactcag aaaatatcgg ttttgctatc ccaacacaaa
     4441 aagatcaagc tgtctatggc acagtatctg gattgacgat ttcttctggt ctagacgaga
     4501 aacaaaaaga ggctgctgaa gcattcacag ctttcttatc tcaaccgaaa aacatggaaa
     4561 agtgggtctt gatgtctcct ggtggtgctc aaccagtgaa taaacaagta gtcgaactgg
     4621 atggttacaa agaaaatgaa gtcatcaagt cttttggcga gctgccatct gagattgcta
     4681 gtgcttttga tgaagtacaa gtctttggac tagtcgggga aaagaatttc acgaaaatgg
     4741 gagatatcac aagctcaggt gtgatcggaa aagcagtgaa ccaagtaact gtcggaaacg
     4801 aagatgtcga tcaagcttta gctgacgcac aaaaaaatac gaaataacaa gaagttctgc
     4861 caatggtgtt cgaaaaaagt agatagctat tggcagatta tcagggggag gtttcaatga
     4921 atcgtccaag gaaacattct gacttaaaat tagccatggg acttttagca ccaagcatgc
     4981 ttttattagc tgttttagtg ctgattccaa tgctttccaa tattcagatc agttttttcg
     5041 atatgccgct gaatcctaac tcagacgccg tttttatcgg attaagcaat tatcttgaaa
     5101 ttttaagtga tagtgaattt tatcgttctt taggtttaac agttgcttac acattcttag
     5161 ttgtagcagg cagtacagct ttaggattag ccgtagctat tttgcttaac cgcaagttca
     5221 gatttaggaa aacggtgcgt tcactgattt tattaagtta tgttacgcca tctatttctt
     5281 tgatttttgc ttggaaatat atgttcaata atagttatgg aatcgtcaat gatctgttag
     5341 tagataggtt gcagcttttc aacgaagcac cgctgtggtt cgaccagcca gttgctagct
     5401 tctttatggt cgttttgttt gctatctgga gatactatcc gtatgcgttt atttcatttt
     5461 tggctatttt gcaaacagtc gacaaaacgc tttatgaagc agctgaaatg gacggtgcaa
     5521 atgcttggca gaagttcaag atcgtgacac tgccggcaat caaaccagta ctagcaactg
     5581 tgattgtttt acgatctatc tggatgttct atatgtttac agatgtctat ctactgacga
     5641 ataaagtcaa tatcttaggc gtttatttgt atcagacagc ctttgcgttc aatgatcttg
     5701 gaaaagcagc agctatatcg attctattgt tcatcattat tgcagcggtc gtcttgctct
     5761 cacgaaagaa ggtggacttg aaatgaaaaa taaaaagagc aggaaaatct tattttacgc
     5821 gacgattttg ttgctattag ctgtcaattt gtttccattt ttgatcatgc tgatgacatc
     5881 attcaaggat tcacaagaag caattgcaac gacacaaact atttttccga aagaatggac
     5941 ttttgaacat tatcaggata ttttcaatgc tgagattttt ccattcatca tgtatttcaa
     6001 aaatagttta gtgatctcct tgatagcctc cgtaactgca gtagtgattg ggatcagcgg
     6061 ggcatacgct ttgtcaaaac tacgcttcaa agctcgctcg aaaatcaatg ctagctttta
     6121 caccgtttat atgttttccg gtattttgat cgttgttcca ttatttaaaa tcatttccgg
     6181 tctaggtttg tatgatacca atacagcact gattatcacg atggtcgttc aaacacttcc
     6241 tactgcgata ttcatgctaa aaagttattt tgacacgatt ccgactgata ttgaagaagc
     6301 agcaatgatc gatggtctta gccggatcca gatcattatt cggatcatta taccattggc
     6361 tatttctgga atcatttctg tcttcgttta ttgctttatg gtttcgtgga atgattattt
     6421 atttgcttcg attttcctgt cgacctccga aaaatttact ttaccaatcg gtttgaacac
     6481 attattcagt acaccagact acatttgggg acgaatgatg gccgcttcat tggtgaccgc
     6541 tttaccagtc atcattatgt atgcgttatc tgaacgattt attaaaggtg atttagtcga
     6601 aggcggcgta aaaggataaa aagaaaagaa agaggaaaga aaagatgaaa aaattagttg
     6661 cattagaacc aagaatagcc ggattcaaag attacgaaga ccgtgagatc gaaaagaacg
     6721 aagtacgtat ccgagtaatt ttcgctactc caaaaccagg aacagaaatt gtcgacttta
     6781 gaggattaag tccatttatc gatgaagaat tttcagaaga atggcgccta ttcttgccaa
     6841 gagaagaagg aagccaacgc ggtgtggtat ttggcgaatt tcaattagga aatatgattg
     6901 tcggtacgat cgaagaaacg ggcagtttgg tgacagagta tcaaaaagga gagttagtct
     6961 gcacttatgg acccattcgt gaaacgctga ttacaaacgg tgttgataat tataaactac
     7021 gtaaacttga tcggttggat caatggaaaa atgcagtatg ttacgatcct gctcaattcg
     7081 ctttaggtgg tatccgggaa gcacatgttc gagcaggtga tgctgtagca atcttcggtt
     7141 taggcgctat cgggcaaatt gcgattcaat tagctaaaaa agcaggtgct actaccatcg
     7201 ttgggatcga tcccatagcg cagcgtagag aattagcaga aaaatacggt gccgattaca
     7261 cgctagatcc tatgaaagca gatgtcggtt ttcgtctaaa ggaattgaca gataaacgtg
     7321 gagtagattc agttattgaa acgagtggca gtgctgccgc attacaagct tctttaagag
     7381 ggatcggcta tggcgggatc atttcttatg ttgcctttgc taaacctttt ccagctgggt
     7441 ttaatcttgg tcgcgaagca catttcaata atgcaaaaat cgtctttgct cgagcatcta
     7501 gtgaaccaaa cccagattac ccaagatgga accgtaaacg aatcgaggac acatgttggg
     7561 aattattgat gaacggttac ttggattgtg aagaaatcat tgatccagtt gtgccatttg
     7621 cagaaagcgc agaagcttac atgcgttatg tagaccagca cccagaagaa agtatcaaaa
     7681 tgggcgttca agtccgagga ggagaacaat gaaattcggt acgcaagatc aagcattttt
     7741 cccaagagac attttagaaa aatttagatt tatcaaagac tgtggatttg aagaatacga
     7801 aatcgacggt aagttgttaa tagaaaatat cgacacagtc aaacaggcaa tcaaagaaac
     7861 agggatccca gtagtgacag cttgtaacgg ctacgatggg tggatcggtg actttatcga
     7921 agaacggcgt cttaatggtg tacgtcaaat tgctgagatt ttgcgtgcac taagcgaggt
     7981 tggaggcaaa ggaatcgtta tcccagcagc ttggggaatg ttcacttatc gattaccgcc
     8041 gatggtttca ccccgaagca aagaaggaga ctataaagca gtcagcgaat cacttcgtta
     8101 tttagataaa gtagccgaag aaacaggtac ggttatttat ttggagcctt tgaaccgata
     8161 ccaagaccat atgatcaatt tgttagcgga tgcgcgtcat tacattgact ctaatcaatt
     8221 gaaacatgtg cagatcatcg ctgattttta tcatatgaat attgaagaag atgatatttc
     8281 aaaagcattg actacgcaaa aagagtatgt gggtcatatc catttagcag ataatcatcg
     8341 ctaccaacca ggatctggcg caattgattt tactcgccat tttcaaacgc tacttcaaaa
     8401 tgagtatgaa ggttatatgg tatatgaatg tcgtttgcga ggagaaaatg aagtgaaaat
     8461 ttatcaggaa tcattgcaat acttgaaaaa ctgtctggaa acagcgcgtc atttaaatga
     8521 tttagagaaa gaatagagaa agaataacaa taagtaaata agcaaaggag ggagaaataa
     8581 atgaagaaag tagcaatcat aggcgctgga caagtagcag aaaaagtaca tgctgcttat
     8641 tatcaaaaaa gatcagatgt cttagaattg gccgctgtgg ttgatccagt cagccagcga
     8701 gcagaagact tttgccgacg tacaggattt tctcatgcgt atacatcagt tacacaaatg
     8761 ctgaatgaga tttctcccga tatcgtgagt gtctgtacac ccaatcgctt tcacttcgaa
     8821 catgtattgg ctgccctcga agctaatgct tcagtgatgt gcgagaaacc accagctatg
     8881 acagctgatc aagcaagaaa gatggcagag acagccgata aaaacgggca gattttggct
     8941 tatggattcc agcatcgttt ttctgacgaa gcaacaatga tcaagaatca tcttgatgtt
     9001 ctcggggata tttattacgt agaagcgaat gcgcttcgtc ggtctggtgt tccaggctgg
     9061 ggaacgttta tcgacaaaga acttcaagga ggcgggcctt tgatcgatta tggcattcac
     9121 atgcttgata cagcgatgta tattactgga tttcctaaag taaaaagcgt tcgggcgtac
     9181 agcttccaaa aaattggtac gaaaaaaaat tcagggaaat tcgggaaatg ggatccaagc
     9241 aaattcacag tagaagatgc aatttttggc acactggaat tagaaaacgg caagatcatc
     9301 caactgaata cttcgtttgc tttgaatatc agagaagaca aactgaatgt ggctttttgt
     9361 ggcgatcagg caggtgctaa tctgtatcca ggcgaactat tcgaagatgt agatggaaag
     9421 ctggtttcct tacaaaagac cacgccagtt aatgacccac atgaactaag tatggctcat
     9481 tttgtgaatc gggcgctcgg tgaccagact cagatgattg cagatggctg gcaaggatat
     9541 gtcatccaga atctggtgga aaatttatat cgttcggcag aaacaggaga agttattacc
     9601 ttatgaaagt agaagaaaaa cagtttgatt tagagtatat caataagtat gcgagtcaga
     9661 tgaccattgg aaatggattt tttggtatca gaggaagcca ggaggaagac tatcggaatc
     9721 aagtaagggg aatgtttgca gctggtgtat ataaccgacc tttaggagca gaatcagccg
     9781 agttagtgaa tctcccagat gtcatcagat gggaaataaa aatcgacggt gaacattttt
     9841 ctcttgagac gatgaaagtt cttgcatacg agcgggcact agatttagaa atgggagagc
     9901 ttaaacgcta tgcgctcatt gaaactgctc aaggaaaaaa agtggaagtc attacttgtc
     9961 gtgttgctga tcaaaataat ctccatcagg cagccctgaa agtggtcatc aagccgttga
    10021 ccgaagactg ccaagtagat gtgaaaacgg gtatcgatgg acagcaaacc aattttggta
    10081 cacagcagtt ggttgaaaaa gaactgcgag tatttggtga cgatttgatc agtgcatcct
    10141 accagacaac agaaagtaaa attgatattg ggattgcagc taaattcaat aaagcaggtg
    10201 tattccaagc aaaaaatcgc cggatcaccg cttccttttc agaaaagatc agaaaaaata
    10261 cgtcctttac gctggagaag atcatcgtgc tctctacctc tcttcagcaa acagacccgt
    10321 ttaagaaaag tattgcccaa gcccagaaaa gcgttagtta tacagaaatt ttagagcagt
    10381 cgatgtctta ttggacagat ttttggcatc agcatcggat aacgatccaa ggcgattctt
    10441 ctttcgatca gttagcgatg gacttcgctg cttaccattt acagattatg actccccgca
    10501 atgatccaag tgttagtgta ggggcgaagg gactaactgg cgaaggttat aaagggcatg
    10561 ttttctggga tacggaaatt tttatcctgc ctttcttttt atatcagttt cctgaagtag
    10621 caaagaattt gctgctgtac cgttatgatc gattagaagg cgcacggcaa aaagcaagag
    10681 attatcacta tcaaggtgca cttttcccat gggaaagcgc acacactggt caggaagaga
    10741 cacccaaatt tgctgcattg aatatcaaaa ccggaacaag acaagaagtt gcttcagctg
    10801 ccgcagaaca tcatatcagt gcagaccttg cttatgccgt tcaagagtat gtccaagcaa
    10861 cagaggatca atcgttttta gaaacaaagg gaaaaacgct cattcatgaa acagcagctt
    10921 tttggctgag cagagctgta aaagtcgatg atcatttaga gatacacaga gtcattggtc
    10981 cagatgaata cacagaatat atcgacaata atgcatatac gaactatctg gcgcattata
    11041 atgtggcctt agctgttaaa tatcaagctg gcgatccgct ttttttgaaa gaatgtcaaa
    11101 attttttaga taagttatat ttgccagtgc ctaacaaaga acagctgatt ccgcaagatg
    11161 acacgtatct cagtaagcca gttattgatt tgaccactta caaagaacgt cctggcagcc
    11221 agtctatttt acttgattat gcccgtagcg aagtaaatga actccaagtc agcaagcaag
    11281 cagatcttgt catgctgtta tacttattac ctgagtattt ttctcaacca atcgtttcta
    11341 agaatttgca ttactatgaa aacaaaacaa tccacgactc atcgttgagt aaagcgattc
    11401 acgccataga ggcttgccgt atccaagaaa tggaatgggc gtatcattta ttccaagaag
    11461 cgtgcctgat tgatttagga ccagatccgc attcatcaga tgacggaatt catgctgctt
    11521 cattaggagc actatggcta gcagttatat ttggtttttc aggtattaca gtaacgaatg
    11581 gaactctttc tatttctcca agacttcctt cagcatggga aaaaatttct tttcctttta
    11641 tttggaaaaa tcacaggttg ctatttgttt tgacaaaaaa acaaatagta ataaaaaaag
    11701 agacgaaaga aacattacct atttttgtca atggagacca ataccagctg gaaaaagaac
    11761 tgatcttgga acggagggga aaataatgcg tattctgatt gcgccagatt ctttcaaaga
    11821 aagtctgaca gccttagagg cagcacaagc cattcaatca ggttttcaag aagtttttgt
    11881 tcaggctgaa tatgatctgc tacctatagg cgatggagga gaaggaacgg tttcatcatt
    11941 agcagacagt ttaaggttga agcggcaaac agcagtcgtt actaatgcat ttggcaaaaa
    12001 caaagaaatc cagtacgctt cagacggtca aactgcgatt tttgaaatgg cagaaatcgt
    12061 cggattgaaa gatgtcccac taaaccaaca acagccactt aggttatcca cgcgaggagt
    12121 aggagaactg atacgacaat tgttgcaaca aggtgttcga cacatcatca tcggcgttgg
    12181 cggaagtagt accaacgatg gcggcatcgg catggcagaa ggtctaggtt atgcttttta
    12241 cgatcaagat aatcaaaaag tagaagcact gggagaaaat ctaggtaaaa ttgctacagt
    12301 tgataaaacg aacgtgccta ttgagctgat taaagccaaa atcactgtca ttactgatgt
    12361 tactaatctt ttatgcggaa aagaaggcgc ttcttttgtt ttcggaccac aaaaaggctt
    12421 gaaagaaaat gaattagcag tggctgacca agccatgcat gatttttata tgagatttgc
    12481 ccctcaagta atcgaggcat cgggcagtag tgctggtggt ggaatgggtg caggactgtt
    12541 agcatttgct tcagggaaaa tagtctctgg catcgactac gtattggatt tactggagtt
    12601 cgataaacgg gtacagcaag cagacctcgt aatcgtaggc gaaggacgtc tagatggaca
    12661 aagtctaaaa ggaaaagcgc cagttgggat tgctagaaga gtaccagagg gcgttcctgt
    12721 gatagctatt tgcggaagtg ttggtaaagg aagcgaaaaa gcagcagatg ttggcatttc
    12781 ggccgttttt ccgatccttc ctgcagctgt gcctttacaa gaagctttac agcaagccac
    12841 tgataatctt catcgcacag ctaaaaacat cgcatcttta cttcaagtcg ttcattttca
    12901 aggaggtaat cgatgaaaat caaagcagca ctattcgatt tagatggcgt tcttgtagac
    12961 acagctcgtt atcattacga agcgtggctg gttttagcta accagctgtc cattccattc
    13021 actgagaaag aaaacgagca actgaaagga atatccagaa ccgagtcact agagcgattg
    13081 ctgtcatttg gaaaaatgga acagaaattc tcagaaaaag agaaatcagc ctttgctgag
    13141 caaaaaaaca atctatatct tcaagcgatt caaaaaatgg atgaaacatc cgttttgcct
    13201 ggtgcaatag ctgttcttga gtacttgaaa aaaacaaaca tcaagatcgg attgggatca
    13261 gcaagcaaaa atgcacgcct cattttagaa aaaacgaatt tgacaagtta ttttgatgtc
    13321 cttattgacg gcacgcaagt atcaaaagca aaacctgatc cagaagtttt tctcaaagga
    13381 gcacagcaat tgaatgtacc gccaaatgca tgcttggtca ttgaagattc agaagctggt
    13441 tgtcaagcag ctttggctgg taacatgcat gtattgggaa taggtgaaaa cataaatctt
    13501 ccttctgctg aatatgtgat tcctgattta accgtttttg atcaagtacg ttcgttttgg
    13561 catttatcag aagccgtatc acgtatataa tagtttccgt tttttctgtc acaagcaaaa
    13621 cgttatcctc cttttggaaa tcaaggtatg atgagaaaaa agggggattt tatatgacaa
    13681 aaaaagcagt tttcgcaggc ggatgctttt ggtgtatggt caaaccattt gaagagcagc
    13741 cagggatcat ttctgtgaca tccggttata ctggcgggca tgtaccaaat ccaacttatg
    13801 aacaagtaac aacaggcaca acagggcata cagaagcagt agagatcgaa tatgatccag
    13861 aagttatttc ttatgatcag ttggtggaaa tctattggca gcaaaccgat ccgactgacg
    13921 cattaggaca gttcgctgat cgcggtgatt cttatcgtcc agtgatctac tataatgatg
    13981 aagaagaacg aaatattgca gagcgttcaa aacaagcatt gcaagacagc gggcgattcg
    14041 ataagccaat cgtgacaaaa atcgaaccaa gaagcgaatt ttatcctgca gaagattacc
    14101 atcaggatta ttacaagaaa aacaagctcc attacaatct ttatcgagaa ggttctggac
    14161 gtgcaggttt ccttcgtcga cattggggcg tcaagaaata agaagccgaa aaagctactt
    14221 atggaacacc gtatatcaga ggttgtaaaa ccagcatttt gagctaatac tgagggtcag
    14281 ctgattgaac accatttagt ggagaggttg tgaacctgtc gcctagctcc aagtcacaag
    14341 gatttggtaa gagaacacta tatatcagag gttgtgacaa gtcgtttgtc acaacctttt
    14401 ttcaatacct aagatgaagg acaatcagca acgcactgct caaaaaagga atgaaaggca
    14461 gcttttccaa ctttttttta tgacagataa agtaaacaga aaaaacggat attccaaaca
    14521 gactagcaag aaacaagatc tgaagcaact cttctagaga caagccacca ctccaagaaa
    14581 gcatcaacag agtatcgcca gctccgagat aggattgacc atagcgaagg cacacaccgc
    14641 ctacgataaa cgttaacagc acactttgcc attggaagac gccggcctgc aaccaaaaaa
    14701 tccataaaat tgcccaagaa cagaatagcg tgtgagaatg gaccatgaag taaaataaat
    14761 ccatcaacga aaatagcaaa gccgaaagca tccatatgaa cgtcgaaaaa tttcttgcgg
    14821 aagaaaaagt aaagaaccag gcaaaaagac caccacataa ggcttctgcc aacaaatagt
    14881 agaagggcaa ccgacacctg caatagcggc agcgaaatcg ttggacgatg atcgagagaa
    14941 tcggaatcaa ttcataccaa gaaagcgaac gacagcattt gacacaatga gatctaggat
    15001 aaataataga gtgacctaac ggaatccgct gggcggttaa acataaaaat gatccgatac
    15061 agcaaccaat gataaaataa agtaacacgc catctccccc ttttcaaaac caagatacgc
    15121 aagcttaaac taaatttttg tacatacata aaatttgagg tgaaaaaatg aacgtttcaa
    15181 tcggattaga tatcggaacc acacaaacca aagcagttgc tttcacagat gaagcccaag
    15241 aagcagcatc cgcttatttt cgctaccctt tgatccaaga aacacaaggg atggcagaac
    15301 aagatcagga attgatcttt caaggtgtct gcaccgttat aaaagaagta attaaacagt
    15361 tgccttcaga cacaatcatt caatttgtct ccttctcctc tgcgatgcat ggcctgatat
    15421 tgttggatga agagaaaaca ccgttatctc gaatgatcac ttgggcagat aaccgagcag
    15481 cggacgaagc agaaaaactg aaatataccg tgcttggcaa agaattttat cggcagactg
    15541 ggacgccgat tcatccaatg acaccattga acaaaatcaa gtggctgcaa aacagccaac
    15601 ctgaattatt caaaaaaact gcttattttg tcggtatcaa agactatatc ttttatagat
    15661 tgtttggcga actgatcagc gactacggaa cagcttcagg aactggatat tttgatatcc
    15721 atgaattcca atgggcagaa gaagtgcttg actacttgaa cattcgaata gagcaattgc
    15781 cgcaagtctt tccttccacc tatcaagtaa caggtctacc atcaaaaaca gcagcagaac
    15841 taggtttgcc aaatgatata tcatttgttt tgggaggggc agacgggccg ctttctaatc
    15901 tagggttagg tgcttttggt gatacgatcg ccgctttgac cgtaggaacg agcggagcac
    15961 ttcgttttat tactactcag cctcagttcc atccagaaat ggagacgttt tgttatgttc
    16021 tggatcagaa acattgggta gtgggaggag cgactagcaa tggtgctggc atctttgatt
    16081 gggcagggca tacattttta caggaagtcg ttcaatctgc taaagaaaaa ggagaaaatc
    16141 catatgatgc actgctttcg gctatcgctt ttgtgccagc gggtgccaat ggtctgattt
    16201 ttcatcccta tttacttggt gagcgagcac cgctttggga cgcagatgcg tttggcagtt
    16261 ttatcggttt acagcggcaa catacagaaa aagaaatgat gaaggcgatt ctagaaggga
    16321 tctgtctcaa cctttatcgg attttaaaag gagtttgcca ggaaaatcag ctcacagaga
    16381 tccgtgctac aggcgggttt gctcaatctc cgatttttcg tcagatgatg gctgatgtac
    16441 tgggttaccc gcttgttttc cctactgtca cagaagcatc cgcattagga gctgtgattt
    16501 taggctggag aagcatcgga aagatccaaa gtatcagtga agcacaggaa ttgatagaat
    16561 tgaaagagca agttggagta caagaaactg atcataaaaa atatcagaaa atctatccat
    16621 tatttgtttc gacacaaaag catttagcac agacttatca gcaatttgct caactgcgag
    16681 aagaattagc tgattctgaa aaatgattgg ctttacggat aaaaaagcga actgaaaatc
    16741 gttctcaggt aaatgatttt gttgtacttt tcattgaaag tctactatta ttttaagtgt
    16801 aagaaaatgt taggaggcaa gaaagatgaa attccaacaa accaaagaag tattaaatca
    16861 attagttgca gatctaagcc aaatgtctgt tgttgttcac caaacacatt ggtacatgag
    16921 aggaccaggt ttccttacat tgcacccaat gatggatgag ttcatggatg atttgaacgc
    16981 tcagttagat gaagtatccg aacgcttgat cacattagac ggttcacctt attctacatt
    17041 gcgtgaattt gcagataaca caaaaatccc tgatgaaatt ggccgctggg atcgtactat
    17101 tccagaacgt ttggaaacac ttgttgcagg ctatcgttat ctagcagatc tttaccaaaa
    17161 aggaatcgaa gtttctggag aagaaggcga cgatccaaca caagatatct ttatcgaatt
    17221 caaaacagcc acagaaaaac gtatctggat gatccaagct catttgggca aagcgccaga
    17281 gatcgacgcg taaaaactgt tgttgaactt gaccgaaaaa gaggttgtga aatcagcgtt
    17341 ttgacctgag tattagagaa acaaacgtca aaatagttcc tcatattttg ccgttttttg
    17401 agctaatacc gagggtcagc tgattgaaca ccgtttacca agaggttgta agcctgccgt
    17461 tcagctccga gtcacaagaa acaattttaa aaaacagctt cttatgtttt atggatattg
    17521 tgacttgtga ccgaggagtt ggcaggagaa caccgtttat tcaaaataag gggttgtgac
    17581 aagacttttg tcacagcctc tttttgtttt tcattttgac aggaaacgac ccttttatat
    17641 aaaagaaaga aaagaagtaa agtttcatat aaatttttca atcaagcaga aaaacgttga
    17701 cattgactag attagcgggt aatatgttaa atggtattgt ttgccccaca aagagccccg
    17761 gaaactcgaa gtgtatgtca aacatcagaa cttaaattgg aggaaaataa cgtgcgtaca
    17821 acatatatgg ccaaaccagg cgaagtagaa cgtaaatggt atgtagtaga cgcaactgat
    17881 gttccattgg gacgtctttc aacagttgtt gcatctgtac tacgtggtaa aaataaacca
    17941 actttcacac ctcatttgga tactggcgat ttcgtcatcg tcatcaatgc agacaaagtg
    18001 aaattaacag gtaaaaaagc gactgacaaa atttattacc gtcacagcaa ctacccagga
    18061 ggattgaaat caatcactgc tggcgaattg cgtgctaaaa attctcgtcg tttgatcgaa
    18121 acttctgtaa aaggtatgct tcctaaaaac actttaggcc gcaaacaatt tacaaaattg
    18181 aacgtatacg gtggagcaga acatccacat gcagcacaac aaccagaagt tttagacatc
    18241 acaaacttaa tctaatagga gggaatttca ttggcacaag ttcaatatat cggcacaggc
    18301 cgtcgtaaaa acgcagttgc tcgcgtacgt ctagtaccag gaactggcaa aattactgta
    18361 aataaaaaag atgttgaaga atacattcca catgctgact tgcgtgaagt aatcaatcaa
    18421 cctttcgctg ttactgaaac aaaaggcgca tatgacgtat ttgttaacgt aaacggcgga
    18481 ggatacgctg gacaatcagg agctatccgt catggtatcg ctcgtgcatt gctagaagtt
    18541 gatcctgact tccgtttagc tcttaaacgt gctggcttac ttactcgtga cgcacgtatg
    18601 gtcgaacgta aaaaaccagg tcttaagaaa gcacgtaaag cttcacaatt ctcaaaacgt
    18661 taatatcctt tatatatcaa cgtttctggc acttttcaat tatttggaaa gtgcttcttt
    18721 tgtttttaag aacacattta agaacacata tcaatcacac ttattcaatc ctctctgaaa
    18781 agcttcaaaa acagccttat caactatatc tggtgtgttc acatatatct ttgttgtaga
    18841 aatatctgag tgtgttaaag catttgaaat ttgattcata tctgctccac cttgtctagc
    18901 tatagttgca taagtatgac ggagtttgtg tggagataaa tgaactaagt caggatgcct
    18961 tcttttaatt gaattgattc gatagtttag gtaatcggca tgaacaggac aatttacttc
    19021 tccagatggt ttagtataag taaagagata ctgatctaac gtttgcttaa tttttaattt
    19081 caacaattga tcagcttgta ccgatttcca ttcacttagc aatgtcatta caacttctgg
    19141 tacttgaatg acggtatctt ttcgaccttt tgtaaacttt tttctttgat atttatctaa
    19201 agtatgttta agacggaccg tttgtttttc aaaatcaata tatttccatt gcaaagcgta
    19261 agtctcactt tttctatcac caagatacag ggtaatcata aaaagtgtgt aatcatgaaa
    19321 agtaagtaaa tgattattaa aatcatcatt tactgcttct atccaattag taagttcttt
    19381 tgatgatagt gcttgcttac cttcttgtat tcttttttct tctaaagcat tttttttagg
    19441 tgcagtgata ctttgaataa ttttagtagt tcgattaaat tcgatataat ttagtatttc
    19501 tgcaatatcg aacatggaac gaacatatcc ttttatgatt tttatatttg catattcttt
    19561 cgattttttg gttaattcat ctgatattat ctcagtattg atatttagat aattcattgc
    19621 gtattcacca aacataggta aaatatgcaa ccgaaatata tcttttgtat tactaatggt
    19681 tatatcacta ggaggcctgt ttgatctaat tgtttgacct aattcatatc taggaagcca
    19741 tttagattcg taaaattttt taaacgttat ttttcctttt aattctaatg agtttgcatt
    19801 ctcctctctc aaaacttttt ctattttctt cttcatatca ttttcggctt taatcgccaa
    19861 ttgcctattt ggaaatgttt tttgatactt ttctgatgca attcctgtta tttctttaaa
    19921 cctttttgga tatctcagat tagttttata tttcccattt gaaagctttg taggcaaaat
    19981 cctcactcct taaaaaaagt aaggcagtat atttcactaa ttttagtata tgcggcgaga
    20041 agaaaatttc aagtattcat aaagtcttta atatcttcta cgtcaaagcg aactacacca
    20101 ccaactctta ttactgggaa attatatata cgaatccact tatctagagt attattatcg
    20161 attcctagaa atgcacaggt ttgtttttta ttcaggtaga ctctggattc tataaaatca
    20221 tctcctaaaa tagtagttat gaaatttttg aattgctttg ggtaagcaca taggaatttc
    20281 acctctcccc agtttacggc gagccttacg gaagtatcat tatttgctgc tattgtcatg
    20341 gcattttgtt ttcccagaac aaaaagaaat ctctaagtat ttgtcgctaa tcaggtcatg
    20401 gcgtacttga gtttctagct cgaatagcag ggatcgttat tcaaaaattg tatttaattg
    20461 tcaatgatca aaaatagttt ttttcctatt cactattaat aacgataaac atgtataaaa
    20521 agacacacta cgaaaaaagt taaatggagt ttactgattt tcatatttgt atatggaaca
    20581 gacatagtct tgaataattt ttataaatgt tttgatgctt atcaacttat tcaacccgct
    20641 atccacttca agtataattt ttccatacgc caataaagta gttttaggag catgttctca
    20701 tttgagaacg ctcctttttt atatattttg aaagctactt actatacgcg tagtttcaaa
    20761 tagttttgtc attctaatct tcagcaacag aaaaaaatct aaact
//
LOCUS       DAZKNH010000047        20360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000047 DAZKNH010000000
VERSION     DAZKNH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.047"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            291..1430
                     /locus_tag="ACG8MG_001571"
     CDS             291..1430
                     /locus_tag="ACG8MG_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFQ0375618.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="ACG8MG_001572"
     CDS             complement(1497..2072)
                     /locus_tag="ACG8MG_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0375619.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="ACG8MG_001573"
     CDS             complement(2077..3051)
                     /locus_tag="ACG8MG_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFQ0375620.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="ACG8MG_001574"
     CDS             3334..4302
                     /locus_tag="ACG8MG_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0375621.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="ACG8MG_001575"
     CDS             4415..4693
                     /locus_tag="ACG8MG_001575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0375622.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="ACG8MG_001576"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="ACG8MG_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFQ0375623.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="ACG8MG_001577"
     CDS             5150..5905
                     /locus_tag="ACG8MG_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0375624.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="ACG8MG_001578"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="ACG8MG_001578"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFQ0375625.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="ACG8MG_001579"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="ACG8MG_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFQ0375626.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="ACG8MG_001580"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="ACG8MG_001580"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFQ0375627.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="ACG8MG_001581"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="ACG8MG_001581"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFQ0375628.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="ACG8MG_001582"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="ACG8MG_001582"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFQ0375629.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="ACG8MG_001583"
     CDS             10642..11184
                     /locus_tag="ACG8MG_001583"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFQ0375630.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /gene="atpA"
                     /locus_tag="ACG8MG_001584"
     CDS             11207..12763
                     /gene="atpA"
                     /locus_tag="ACG8MG_001584"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFQ0375631.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="ACG8MG_001585"
     CDS             12777..13679
                     /locus_tag="ACG8MG_001585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFQ0375632.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="ACG8MG_001586"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="ACG8MG_001586"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFQ0375633.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="ACG8MG_001587"
     CDS             15122..15544
                     /locus_tag="ACG8MG_001587"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFQ0375634.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="ACG8MG_001588"
     CDS             16013..16243
                     /locus_tag="ACG8MG_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFQ0375635.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="ACG8MG_001589"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="ACG8MG_001589"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0375636.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="ACG8MG_001590"
     CDS             17654..17830
                     /locus_tag="ACG8MG_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0375637.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="ACG8MG_001591"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="ACG8MG_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFQ0375638.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="ACG8MG_001592"
     CDS             18325..18708
                     /locus_tag="ACG8MG_001592"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0375639.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="ACG8MG_001593"
     CDS             18877..19761
                     /locus_tag="ACG8MG_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0375640.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19875..20111
                     /locus_tag="ACG8MG_001594"
     CDS             19875..20111
                     /locus_tag="ACG8MG_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFQ0375641.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="ACG8MG_001595"
     tRNA            20199..20279
                     /locus_tag="ACG8MG_001595"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="ACG8MG_001596"
     tRNA            20286..20357
                     /locus_tag="ACG8MG_001596"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3566 c         4425 g         5610 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat
//
LOCUS       DAZKNH010000048        19982 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000048 DAZKNH010000000
VERSION     DAZKNH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.048"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(226..1209)
                     /locus_tag="ACG8MG_001597"
     CDS             complement(226..1209)
                     /locus_tag="ACG8MG_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFQ0375642.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1214..1861)
                     /locus_tag="ACG8MG_001598"
     CDS             complement(1214..1861)
                     /locus_tag="ACG8MG_001598"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0375643.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1946..2578)
                     /locus_tag="ACG8MG_001599"
     CDS             complement(1946..2578)
                     /locus_tag="ACG8MG_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0375644.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2594..3145)
                     /gene="coaC"
                     /locus_tag="ACG8MG_001600"
     CDS             complement(2594..3145)
                     /gene="coaC"
                     /locus_tag="ACG8MG_001600"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFQ0375645.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(3142..3879)
                     /locus_tag="ACG8MG_001601"
     CDS             complement(3142..3879)
                     /locus_tag="ACG8MG_001601"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFQ0375646.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3876..4865)
                     /locus_tag="ACG8MG_001602"
     CDS             complement(3876..4865)
                     /locus_tag="ACG8MG_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0375647.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            5123..5665
                     /locus_tag="ACG8MG_001603"
     CDS             5123..5665
                     /locus_tag="ACG8MG_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFQ0375648.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5682..6767
                     /locus_tag="ACG8MG_001604"
     CDS             5682..6767
                     /locus_tag="ACG8MG_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375649.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6779..7585
                     /locus_tag="ACG8MG_001605"
     CDS             6779..7585
                     /locus_tag="ACG8MG_001605"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0375650.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7586..8413
                     /locus_tag="ACG8MG_001606"
     CDS             7586..8413
                     /locus_tag="ACG8MG_001606"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0375651.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8410..9483
                     /locus_tag="ACG8MG_001607"
     CDS             8410..9483
                     /locus_tag="ACG8MG_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0375652.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            9911..12607
                     /locus_tag="ACG8MG_001608"
     CDS             9911..12607
                     /locus_tag="ACG8MG_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0375653.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            12672..13172
                     /locus_tag="ACG8MG_001609"
     CDS             12672..13172
                     /locus_tag="ACG8MG_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0375654.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            13184..13678
                     /locus_tag="ACG8MG_001610"
     CDS             13184..13678
                     /locus_tag="ACG8MG_001610"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0375655.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            13699..14508
                     /locus_tag="ACG8MG_001611"
     CDS             13699..14508
                     /locus_tag="ACG8MG_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0375656.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            14505..15326
                     /locus_tag="ACG8MG_001612"
     CDS             14505..15326
                     /locus_tag="ACG8MG_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0375657.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            15377..17476
                     /locus_tag="ACG8MG_001613"
     CDS             15377..17476
                     /locus_tag="ACG8MG_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFQ0375658.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            17473..18591
                     /locus_tag="ACG8MG_001614"
     CDS             17473..18591
                     /locus_tag="ACG8MG_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFQ0375659.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            18630..19508
                     /locus_tag="ACG8MG_001615"
     CDS             18630..19508
                     /locus_tag="ACG8MG_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0375660.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            19654..19851
                     /locus_tag="ACG8MG_001616"
     CDS             19654..19851
                     /locus_tag="ACG8MG_001616"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0375661.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         6100 a         3475 c         4279 g         6128 t
ORIGIN      
        1 agatgcaagc ttttgaacac cgtttattcg gaaaaagagc gtgggacaaa agtaaagaac
       61 acttttgtct cacgctctaa acacgtataa acggcggaag cagaagaaat tccttcggaa
      121 ataagctgaa attcacaaaa atttgaaaag caattttcgt gaatttcttc ttatttcttg
      181 gagttaaacg cttctgtccc agcctctttt ttgatcagca tgattttatt cagttatctt
      241 caaaatgatt tttccgatgt ttttgtttgc ttccatatat tcatgcgctt gtttgactgc
      301 gctgaattgg aatgtacggt caatgattgg ccgaatcttg ccattttcta gaaaaggccc
      361 agctttttgc ataaaagttt gtgcgagctc tgctttatac tgatcacttc taggtgtcaa
      421 taatgtacca agcagttgga cacgtttccc aatcaataca ccaaggtcta tctcaggcac
      481 gatggtccca cctagcatac caatcaagac ccaacgtcca tcaacggcta tagaagctaa
      541 attcttttcc cagtaagaag cgccgatgaa atccaaaata acatcgactc cccgaccttc
      601 agtgattttt cgtacctctt cttgaaagtc ttgtttttta tagttgatca attcatctgc
      661 accaagttct ttacaaaagg cgagtttttc ttctgatcct gcagtaacga tgattttcgc
      721 gtgagaaagc tgtctagcta attggatcgc tgctgttccc acaccacttg ctcctgcatg
      781 gatcaagaca gtttcgcctt cttgtaattt tccaagccag taaagtgttt gataagcagt
      841 caaaaaaact tctgagattc cagcagcaga aacatagtct agcgtatcag ggagaaggat
      901 tgcacgatcg gcaggcatca cagcatattc ggcataacca cctagattga ctaatccgta
      961 tacttttgtc ccaggagtaa aagtggagat atcactttta ttctcaacta cctctccagc
     1021 tacttcgact cccaaaatag ggtagggagg tttcaaagag actgattctc ttttcataat
     1081 atctgttcga ttgacagctg ctgtatggac tttgatcagt aattcgcctt gttttggttt
     1141 aggaacaggc acttcaattt gttctaatac atctacagaa ccaggatgct tgattgaaat
     1201 ggcacgcatg gatctattcc tcctttgccc attcttggaa tgctgtattc aactctttgc
     1261 taccttcaga aagtactaga gtcgtacgag aaatatccgt ttcttccact agagcggcaa
     1321 gtacagcagc aactgtagag cgagtgatcg tttttgatgc cttctcatta ggcttagtta
     1381 attgaatgga agtaacttct tcatcgttag tcaatgtgac tggttgaaga atgacaaaat
     1441 ctaagttagt tcgtttcagc cattcatctg cataatgttt agtaatataa taatctgtca
     1501 tggactcagg ccattttgct ggttcatctg cataaacagc actgaccatc agatagcgag
     1561 agacatgcgc attttcagct gcgatcactg ttttgattgc gccatccaag tctacttgca
     1621 gaagattctt tccttgagag ccggcagcaa aaatcaatac atcgatgcct ttgaatgctt
     1681 cggtcatttt ttccacggac caagttaaat caaaagagac atacgaaaca ttctcttttt
     1741 ttactaatgt ttgggaagca acatctctca cccctgcaaa aatttcatgg gaacttggag
     1801 ctaaatcttt tatcaggtgt cggccaattt gtccgtttgc acctacaaca aaaattttca
     1861 tttggatcat cctttcgacg tcaaatcttt ccattcaata gtaacaccaa aagagaaaaa
     1921 aatcagagat cggctacgat gactatcagt tagtaagaga agggtgcttg aagtatctta
     1981 acaagacatt gcatactgct gcagtcgcaa gacctgcgac gattccttca ggtataccac
     2041 ttgtaaaaat aaccgctaga atagctgaat aaacagcatt taaatttgtt ccgatgactt
     2101 tggcataatc ttgctggaat aaaaagtaga tcatattcat cactaaaatc gtattggtca
     2161 atgaaccgga aagtccagct aagaataaag aaacggaatc tgctttttgt ttcattagtt
     2221 tgcgcagacc tttataaacg aaataaggaa ccaccccaat caagatgcga ggaataaacg
     2281 cgatcagcaa ggctttccag cttccatgtt ctgttccgat gactgggata aacggagaaa
     2341 agacaaaaga caaaggtgtt tggataactg tgctacggat catgctgatc agtccaaatg
     2401 taccgccaag aaaagcacct agtcttggtc ctaaaacgat cgaagcaatg attacaggaa
     2461 tatgcatggt tgttgcattg attggaccga tagggataaa acctaaaggc gtaacagcta
     2521 ataaaatcaa aatagctaaa aacatcgcag ttaatgtaaa gttttttgta tttttcataa
     2581 gtactccttt tttctaagca tttctttcat taagagtcag catgacctgt tcgatgatcg
     2641 tctgaatatc agctaaggca ccttttccaa aatctccaca agctaaaaga gattctcttg
     2701 gttcaatttc ctgatagccg acagatctca atgtttccag attacgctga gtgatcgggt
     2761 tttgatacat attcgtgttc atggcagggg caatcaattt tggtgttccc actggaagcg
     2821 ccattgcgac tgttgaaaga agatcgtctg caatgccgtt tgctagcttg ccgataatgt
     2881 ttgctgtagc aggcgcgatg agaaacaagt cagcttgttt tgctagttcg atatgattga
     2941 ttacctgcgg atttgattct tgcatgacgt ctgtatggac tgcgcgcttc gacagagatt
     3001 gcaaggttaa tggcgtgata aaatgtgtgc tgcttttaga cataatcaca tctacctggg
     3061 cacctaattt gacgaattga cttgtaagat cagcgctttt gtaagcagaa atacttccag
     3121 taacgcctag taagatcttt ttcattttga ttcctccagt ctaattttct cagtaatcaa
     3181 tgcagcaatc tcagatttac tttgagcttc ttccactgtt ccatctttag ataagagata
     3241 gccgtggtgt gtctcgccat ggatttccgt caaatcattt gcgaaaataa aatcagtttt
     3301 gttatttatc agactatttt ttgcgactgc cagaagagat tcctttggta catctactaa
     3361 tagtttgaaa ccaatgattt ttgcttttgg ctgccattca cgtagataag aaatgatttt
     3421 tggtgttttc ttcaaaacga gcacaagatg ctcggtgtta gaagagatct ttgaaacagt
     3481 ggtatctgtc tgttcatttt ccgcaaacca attgtccagt tcttcttgcg ttgaagaagt
     3541 gggtaggttt tttgccagct gttcttctga aaaactaaaa gcaggagtaa aatcactgac
     3601 tgccatactg tggataatcg catcgtagtg ttcttttttc gttagttctt ctaattgtaa
     3661 aaataagtcc agtgcagatt cgatcgtata aatcgttata ttggagtgtc ttttcggttt
     3721 cagtgcttgt ttcgtagtca cgtaatcgat tgtggtatcc ggattggcag ctaaataatc
     3781 agctaatgct tgccctaatc gtcctgtcga atgatttgta atagcacgta cttgatcgat
     3841 tttttcagtc gttcctccag ctgtgattag aatcttcatt tatggtcacc tacatttact
     3901 agtgttcttc cttggtgtgt tccagcaagt agtttagatg ctgtttcagg caattgatcc
     3961 agagtaatct cttggacgac cagttcatct gcaatctgca aatccgccat ctgttgccat
     4021 aaagaaagac gttgatctat tggtacattg acagaatcaa ttccaatcaa ctggatgttt
     4081 cgcagaataa aaggcaagac agtcgcgttt agtttgattc ccccagcatt gccacataaa
     4141 gctactgcac cgttataaga gatcaatgga agaagactgc ttagctgctc gccacctact
     4201 gtgtcgatga catagtcgat ttgctgtttt cctaaagctt ttgttgtctc tggcagaaat
     4261 tcatcaggtc gtacgacttg caaagcacct agacttttta gccatgtgac agactcttct
     4321 tttctggaaa aggtaatgat tgactggaat cctagtttat gtaacagggc tatcgctgta
     4381 ctgcctacac cgccagaagc accagtaacg acaacttttt tatcggaggt cataccttga
     4441 cggattaaag cgttcacaca caacatagca gtaaaccctg ctgtaccaag gatcatcgct
     4501 tgacgcagcg tcatattttt tggtagagga actaaccagt ctcctggtac tttttgatat
     4561 tggctataac cgcctggatg ggtgacgcca agcccaaaac cagtagcaag tacaagatct
     4621 tcttttgaaa aacgtggatt gttactttgg acgacagttc cggataaatc aatccctgga
     4681 gtcatcggat atgttcgtat cacctgacca gactcgctca ttgccaatgc atctttataa
     4741 ttgacatcag aataggcaat ttttaccaag acttcatttt cagccaactc agtaaatggc
     4801 agatcactga ttgctgtatg gaaaacaggt tcctttatta cctgaaaaga tcgaaaagcg
     4861 ttcattcgtg agacactcct tttaaaattt tcttttatca taccataaag cctactcttt
     4921 tattcttgtt cttccgtaaa tttagacaaa aattaggtga aaaataaaga atcaaacaac
     4981 tagaatttat tccaaaagat aaagaaatat tcagcaaaaa aaattttaca aagggttgcc
     5041 tttttcaata aatttcgtat actaatacga gtggtagtaa acaggaagtt tactattaga
     5101 aaacttcgat tcggaggaaa ttatggaaat tggtgagaag ttaagaaatt tgcgcatcca
     5161 aaagaatttg acacaagaag aactaggcga acgcacagat ttgtcaaaag ggtatatttc
     5221 ccagttggaa cgagatttaa gttcaccatc aatggaaaca ttcttttcta ttcttgaagt
     5281 cttaggtgtg actccagaag aattttttca tcaagaagca gcgaatctgc aagtggttta
     5341 ttcaaaagaa gatcatacgg tttatttcga tgaagaaaac gggtatgaat tggaatggct
     5401 cgtggctgat tcgaatgaaa aagaaatgga gcctgtcttg ctgacttttg aaggtgcagg
     5461 agaatataag acgtttgaac cttcattatc tgaaacgttt atttttgtat tagaagggga
     5521 attagagtta agcttaggag agacggtcta tgtggcaaaa gcaggacagt cgatctatta
     5581 tcaagcgaca aaacaacatc aattaaggaa tcatctcaaa aaacgaacaa aagtattgat
     5641 tgttgcaacg caatcatatt tgtaatatag ggggtaaaaa cgtggggaaa ccaatcattt
     5701 catttgatca agtagtgaaa cgctatgatg atgaaacagt attaaaaaaa gtaagttttg
     5761 agatcgaaca aggaaaattt tatacactgc ttggtccttc cggctgtggt aaaacaacga
     5821 tcctacgtat tatagctgga ttcaccgaag ctacagaagg ggacatctat tttgaaggga
     5881 aacggttgaa tgatttgcca gctaataagc gacaagtaaa tacagtcttc caagattatg
     5941 cactttttcc tcatatgaat gtttttgata acgttgcttt tggattgaag atcaagaaaa
     6001 tgtccaaatc agatatcgaa aagaaggtaa aagatgcttt gcgtatggtc cagcttcctg
     6061 gatacgaaaa acgggagatt agtgaaatgt ctggtggtca aagacagcgt gtagcgattg
     6121 cacgagcaat tgtaaatgaa ccgaaagtat tgctattaga tgaaccgtta tcggcgcttg
     6181 atttgaaact acgtacagat atgcagtatg aactgcggga tctgcaacaa cgattaggaa
     6241 tcacgtttat ttttgttact catgaccaag aagaagcgtt ggcaatgagt gatgagattt
     6301 ttgtcatgaa taaagggaaa atcgtccaaa gtggtacacc cgtagatatt tatgatgagc
     6361 caatcaatca tttcgttgct gattttgttg gggagagcaa tatcgtggac ggtgtaatga
     6421 tcgaagataa tctagttgag tttgttggta agcaatttga atgtgtcgat ggcgggatgc
     6481 gtctaaatga accagttgaa gttgttttac gcccagagga tttgacgatc acgacaccag
     6541 ataaaggaaa actagtcgta acggtcgata ctcagctgtt tcgtggtgtc cattatgaaa
     6601 tcatctgtta tgatgaacag caaaatgaat ggatggtcca ttcaacgaaa aaagctaaag
     6661 aaggaagcaa agtaggattg gcctttgagc cagaagatat ccatgtcatg cgtttcaacg
     6721 agtctgaaga agaattcgat gcacgtcttg acagctacga agaatagggg ggacaagaat
     6781 gaaaacaatg cgcaggatct attccgtccc ttatattttg tggctcggtt tattcgtgat
     6841 cgcgcctgta ttaatgatca tttatcaatc tttttttgat atgaatgggc aattcacgct
     6901 gaataattat ctatcttatt ttacgtcggg gacatcgctt tctatgacga tcaattctgt
     6961 atggtatgca tttttgatca cgtttttcac gctgatcatc agttacccga ctgcttattt
     7021 tttaacaaaa ctgaagcaca agcaattatg gctgatgctg attattttgc ctacatgggt
     7081 gaatctgctg cttaaagctt acgcatttat tgggattttc agtgtgcacg gcagcgtgaa
     7141 tcatttcttt gattttatcg ggatcgggcc gaaacagata ttattcacag acttcagttt
     7201 tctgttcgtg gcaacgtata tcgagattcc atttatgatt atgccaattt tcaatgcatt
     7261 ggaagagatg aatccttctt tgatcagtgc tagtagagat ttaggcgcga atagctttga
     7321 gaccttccga cgagtgattt ttccgttatc attaaatggt gtgaaaagtg gtgtccaagc
     7381 agtatttatt ccttctcttt ctttattcat gctgactcgt ctgattggcg gaaatcgggt
     7441 gatcacttta ggaacggcta tcgaacagca tttcttggtt acacaaaact gggggatggg
     7501 ttctacaatc ggtgtgattt taatcattgc tatgttcatc gtgatgctcc tgacaggcga
     7561 gaagaaaaaa ggaggtcgct cataaatgaa aaaatttaaa tggtcgtatc tttatttagg
     7621 gatcgtcttt ttattcttat atgcaccgat cttctatctg attttttatt ccttcaatga
     7681 aggaggaacg atgaacagct ttacaggatt tacttgggaa aattatgaag ctgtttttga
     7741 agatacacgt ttgatcatta tcgtattgaa tacgttcatg ctggctttct tatctgcatt
     7801 gatcgccacg atcatcggga catttggggc tatgggaatc tattacacga aaaaacgacg
     7861 tgcacggaca gcgttgttga gcatgaataa catcttatta gtctctcctg atgttattat
     7921 cggtgctagt ttcttgattt tctttacact actaggattc ggattaggtt ttcagagtgt
     7981 attgctttcc catattgctt tcagcattcc aatcgtcgtc ttgatggtct tgccaaaatt
     8041 gcaggaaatg aacgattcaa tggtcgatgc ggctagagac cttggtgcta ataatttcca
     8101 agtagtcaaa aatatcattt tgccttatct ttctccagga atcatagctg gatattttat
     8161 ggcatttacc tattctcttg atgattttgc ggttacattt ttcgtcacag gaaatggctt
     8221 tacgacgctc tcagtagaga tctattctcg tgcaagacaa ggaatcagtt tagaaatcaa
     8281 tgcgctaagt gcgttagttt tcttgttctc catgatattg gtcattggat actactttat
     8341 tagtaaagaa aaccgtccaa aacggctgaa gaaaaacaga agagtacgcg agggggtggc
     8401 acggctgcga tgaaaaaatt acaatctctg tttttaggta ttctcatcat tattgcggtg
     8461 ctgctgcttg gtgttcgtca acttgaaaaa acaagtggca tgagtggagc aaaggtactt
     8521 accatctata actggggaga ttatatcgat cctagtttga tcacgaaatt tgaaaaagaa
     8581 tatggataca aagtgaatta tgaaacattt gattctaatg aagcgatgtt cacaaaaatc
     8641 cagcaaggtg ggacaaacta cgacatcgct ataccaagtg aatatatgat ccaaaaaatg
     8701 atcaaagaaa aactagtact tccattagat cattcgaaga tcaaaggatt aaataatatt
     8761 gatccacgat ttttagactt ggattttgat ccagataatc agtattcgat tccatatttt
     8821 tggggaactt tagggatcgt atataacgat aaattctttg atgcaaatca aattaagcat
     8881 tgggatgatt tgtggaagcc ggaattaaaa gacagtttga tgctgattga cggtgcacga
     8941 gaagttatgg ggctttcttt gaatagttta ggctattctt tgaatagtaa gaatatggca
     9001 cagctgacag aagcagcaaa taaattgaat catttaacac caaatgtcaa agcaatcgtg
     9061 gcagatgaga tcaaaatgta tatgatcaat gaagaagcct ccgtggcagt gacattttct
     9121 ggcgaagctg ccgatatgat gtgggaaaat gaacatttac attatgtgat cccaccagaa
     9181 ggatccaatc tttggtttga taatatcgtt atgccaaaaa ccgtgaaaaa taaagaaggt
     9241 gcctatgatt tcatcaactt tatgctagaa ccggaaaatg cggcacaaaa tgcagagtat
     9301 attggttatt ctacaccaaa tgaagaagca aaagcgttat tgccaaaaga catttcgtcg
     9361 gacgaacaat tctatccatc tgatgacacg atctcccatc ttgaagtcta caaagatcta
     9421 ggatcgaaat acctaggcat ctataatgac ctattcttag aattcaagat gtataggaga
     9481 taatggcaaa actcagtaag aatggggact tatcaacgtt ctaaagagct aaaaatcgtc
     9541 ctttttctat ctgagttttc caatcttcta ccaaaagcgg atgaaacgcc gctttttttg
     9601 tgttatttta ttttttctac cacgtttttc taccagagaa aagtggacaa aaccattgta
     9661 aaatagggcc cgagacgggc tagtaaatct aagattgaaa aagttacaga aggataaagt
     9721 aattggaaac tatgcaaata tacttaaccc ttctgtaatc tattaataaa actaatatat
     9781 gaatccagtt caatatttgt agctatgaaa aaacataaaa atcatattag ataaaaacta
     9841 aattggtaga taaattattt tgtcagatat ctgaggcaaa tattcgacta tttgtgtttc
     9901 aaagtaaaaa ttggaataca aatatagaat agacatattg cagccgatag tttattatgc
     9961 ttttagacac attaaactgg aggaaaaaat gaaaaagagc gctaaagatc gagtttttga
    10021 tgagctgata cgactaacag ggaatatgtc ttctgtcact gccgagaaaa tttcgaagaa
    10081 tctaggtatc tctcgtcaga atgccagcca ttatttgacg agattagtgg aagataaaaa
    10141 ggtcgagaaa ttaccgggaa agcctgtttt gtggaagcct ttagatgaat atgcggttgt
    10201 agataatacg gagcaaataa acgaagcctt tcattctgta gttggtcatg acggaagtct
    10261 gagagaggtc attcaaaaat gtatagccgc tgtcaaatat ccgcctaacg gactcagtgt
    10321 attaataaat ggagccacgg gtgttggaaa aagtttttta gccacaaaaa tttttgaata
    10381 cgctatccat gagcaaatta ttgaaaaaga tgcaccattt gctatcttaa actgtgccga
    10441 ttatgcggac aatcccgaac tgctttcggc aacactgttt ggatacaaga agggggcctt
    10501 tacgggggct gaaaaggata ccgatggttt attggcaacg gccaacaacg gctatctttt
    10561 tctcgatgag gtccatcgcc tatcgaaaga aaatcaagaa aagctctttc tatttattga
    10621 tactggaaac tatcgtccag tcggtgaaaa tgtaaattgg cattctgcta aggttcgttt
    10681 tattttcgcc acgacggaaa aaggtgaaaa ttatttatta gacacctttg atcggcgtat
    10741 tcaaattagt gttatgttgc caacttttga agatcgcccc attcgagaac gattagagct
    10801 gattcaatta ttttttcaaa atgaagcgga tgttttacaa aaagatatta ttgtttcaag
    10861 agaagccttg tctttaatgt tatctcatcc ctttagtggg aatattggta agctgaagaa
    10921 tatcattaaa atcagttgtg cggatgtgta tagtcgaaca caagaagaac cattaacgat
    10981 tgggaaaaat gagatcttga ttcagttaaa tatgctggaa tccgaagtag aaagcttgcc
    11041 gattgctagt ttattgataa agaaaaatca aaagataaaa acggatcgaa tcaaaccgtt
    11101 tgattatgat tcgattttgg aaaaaattac tcagctgata aatgaaggta atttcaatga
    11161 aaatttttct tcgataaaga atgaagtaca gcatttggtg catcacattg ataaggattc
    11221 ttttttatca atgagtagaa aattgatgat tcagatgttt agaaaggtct gggtatccgt
    11281 tttaggaaaa acgtacggat tgacgacgag tggacctgtt gcagaaatta ttagcagatc
    11341 ctatgtcatt tccccaagct ataagacaga cttaaccggg ataaatcgta cattagctgc
    11401 aaatctacct cggacgactt acttatgtaa tcagttcttg gagcatttac caccgctctc
    11461 agaaaaggac gagaactttc taagactgct tattgtcgtg actttatctg attatgtaga
    11521 tgaatccatc gagctgaagg gcctgttgct tgctcacgga gatagtacgg ctagcagcat
    11581 tcaagcagta gtgaatcaat tatgcggcaa ttatgttttt gaagcgttgg atatgccagt
    11641 agagagtaat gtgtcagaga ttgtcgaatc gacaaggaat ttcgtgaaac ggcaagtgaa
    11701 tacagatagt ctagtgctga tcgtagatat gggctcttta aatcaaattt acacacagat
    11761 caagaacgat ttaaaagggg agttacttct tctgaataat ttgacgactt ccattgcact
    11821 tgatattggc ttgaagatga cgatgaatgc gccatttaaa cagattgcgg aggatgcagc
    11881 agacaagtat tcaatcaatg ttcaatactt tgaaggcttt tctcaaagta gcaacattat
    11941 catttcctgt atgtcgggtt tggggatttc ggacaaattg aaagatgttt ttcgtcatca
    12001 tttatcggat gaatccatcg aagttttcac gaaggactac agagaactac gtgaattaat
    12061 cgaccaaaat gatgaaactt attttgacaa gacaaaattg gtcatcacga cttcggatct
    12121 tccacgttcc ttttcaattc ctaatattaa tgtttatgac atcttagacg gtgatggagc
    12181 agccaatttg gagcatattt tgagtcctga aatgaagccg gcagaattta accgcttgat
    12241 tcaggagcta gtaaaattct tttcgttgga aggaattgcg gaccgtttga actttctgaa
    12301 cccaacgatc gtgatgagtg aagtggaaac agtcatttct aaatatgaaa attactatca
    12361 tttcacttta aatggccgtg taaaactaaa tctctatatg catacggcat tgatgatgga
    12421 acgtttattc ttggctcgga cagaaaaatc tgaagaaccg aattatgatt tgaaaccaga
    12481 tgaagatgaa ttttattatg tttctagaag tatttttcaa tcaatggaga tgaaatataa
    12541 ttttaaagtc aatgcttatg agctttcgct gctctatgaa ttattaggac cgtatattca
    12601 aaaataaagt gtctaaaaaa gtgtctaatc tgtttttttt aggttagaca ctttttttaa
    12661 tgtgaattaa cgtgattaaa ccaatgtttt taaaagttgg cacaggaatt gcttatataa
    12721 aagtgaacag aaagaaagga attaaccata tgaagaaaat tctaatagcg actcacggtc
    12781 acttagcttc aggctttctt agcagtattc aattactgac ggggaaggtt caagagatta
    12841 cggtgattaa tgcttatgtg gatgagtgtg attttgaaca ggagttgaat gtatttgtaa
    12901 accaggtgaa cgagcaggat cagatttttg tttttaccga tttatttggc ggaagtgtca
    12961 atcagaagat taccaaaacc tttttggaga aagagattgc tgtaactatt ttgtctggct
    13021 ttaatctgcc aattctgctc gaaatagtat tggcaaccaa ggaattaaca aaggaggata
    13081 ttcaggaact ggtaaataaa tgccaacagg aattaaagat gaccattctt gatgaggtac
    13141 atgtagtaaa tgaagaggaa gatttttttt aggaggaaga aaaatgatta aagcagtgcg
    13201 aattgatgaa cgattaattc atggacaagt agcgatgact tggacaaaga gtctaaattt
    13261 aaccgggtta gtagtagcaa gcgatgaggc ggctaccaac gatattcaaa agatgacatt
    13321 gaagatggct gcaccatcga atgtcaaagt cattattaaa acagttgatg acgcgattca
    13381 tttgttaaaa gatccgcggg cgaaggacat gcgcttgatg gttttagtcc ctaatgtcaa
    13441 agatgcggta aaagttgcga aagctttccc aacggaaatc gaattaatga acgtggggaa
    13501 tgctgggaaa atgacaccgc catcggaaga taagaaaacc ttatcaaagg aagtgatgtt
    13561 aacgacgtca gaaattcaag acttaaaaga attagttgaa ctttatccag ataccgtttt
    13621 tcagcctaca ccggcgatgg aaaaacgttc agctatctct attttgagtg atttttagaa
    13681 aaaggaagga gaaaaattat gtatgatgca tttgtagtcg ctttggccgt ttttattgga
    13741 gttgctggac atgaaatgtt cgggatgtcc atgctgagtc gtccgattgt tgttgctccg
    13801 ttagcaggag cgttgatggg ggatttgcag acggggttga ttgtcggggc gtcactagaa
    13861 gccatcttta tgggtgtcgt caatatcgga atctctagta cggcggagcc ttctttggct
    13921 gctgggctgg ctacagcgtt cgcaatcaaa atgggaggaa acgtggatgc gattattccc
    13981 attgtctttc cactagcagt attaggatta cagttaatga atatgatttt ttcttttgta
    14041 tgtggaccga tggctgctaa atttttcaaa tttgcaaaat ctggtgatca aaaaggaatc
    14101 atcaatctgc acttctttat gtggtttgtc cactatgcgc tttatgctct tattccattc
    14161 tttgccgttt tatttggagc agatgttgtc aaaagcgtct tggataatat tccagaggtc
    14221 atcatgaacg gattaacagt cgctggaaat ctgctgccgg cagttgggat ggcgatgctg
    14281 ttacggatgc tttggagtaa aaatattgct gtgtggtttt tctttggggc cgttgttatg
    14341 gcgtatctcg agttaccatt gattgcaatt gccgcagttg gaacgattat tgcaattgtt
    14401 atagcccaaa gagatctaca actaaaaaat atatctgttc atgcagttca aagcgatgct
    14461 gctgtaaatg caacgcttga ccaagaagag gaggatttct ttcaatgaaa ttagctgatg
    14521 gaatttctaa agatgagaaa aaattggttc ggaagatgtt ttggcgctcg atgactatgt
    14581 atgcttcggt gaatccggtg acgatgggcg gcggtggttt cgcctattct atgatgccct
    14641 tcattaatca tttctacaaa agtgaagaag atcgagctgc ggcgctggaa cgtcatacac
    14701 agtatttttc gacgaccatt ccttgcgcgt cctttgtcat ggggattgcg gcatcgatgg
    14761 agaaggaaaa ttctgaaaaa gctggttttg acgtggaaag tatcaacgcg atcaaaacga
    14821 gtttgatggg accattggct ggaattggcg attcattgtt ctggggagtt tggcgtgtcg
    14881 ttaccgcggg actagcgatc aacttagcca atgccggaaa tattttggca ccaattatct
    14941 ttttagttat gttcaatata cctaattact tgatgcgata ctatggcggc gttttaggct
    15001 attcattagg ctctaaatat attgaaaagc tgtatgcgaa cgggttaatt gaaattctga
    15061 caaaagctgc tagtatcttg ggtctgatca tggtaggtgc aatgacgatc agtatggtca
    15121 aattcaatac cgttttgagc ttttcaatga agggcgaaga actaatgaat gtccaagaaa
    15181 tcctcgatag tatttttaaa ggaattgtac cgttattggt aacattgagc tgtttcaaat
    15241 tactagataa aaaagtcggc gtcatcaaaa ttatttttgg aatggtcgtc ttgggaattt
    15301 tattgtcatt acttggaatc gcatagtagg aagctgcagc tactaggctg cagttttttt
    15361 agcaaggagg gaaaagatga ctattaatga agtaatttat tcacccaaac acccattttt
    15421 taatgctgag ttaaacgacg gaaatgtgac tacttcaaac gaatacagct actatcggaa
    15481 tattcctgat gtaaagggat tgaccttcga tgtaagtaat caaaagacat tagcaaacct
    15541 tgatgtttgc gggacgattc aaacgttgac cttttatcga gaaaatttac taactgagga
    15601 aaaaccaggt gtttgggtca ataaacagct ctctcaaacc aattcgttat ccttaaaggt
    15661 tgaagtaaac gggattgtct accatttgtc agaatcggat catcgtattg aagtcgatgt
    15721 gattcaagac tgtttgccta gatatattca tcattattcg acgtttaagg tcattgtagt
    15781 atcctttgct cccatactgg ctaataagcg tttaagtatg cttgttcaac aggtttacat
    15841 tgtgaatgaa acacacgaaa gccttcaagt ttcagtgaaa gatgtgcctt cgtatcaaga
    15901 gaagtattcg gatcagcaaa atgtgttgat ttctcaaaaa ggaaataagg acacgatcgc
    15961 acaacaggaa gtggcaagct tcgccattgc ttttgtggac ccaaatgctt ttgaagaagt
    16021 aatgactttt caggaatctg atatcgaaaa atggctgaag gaaacattga actattttgc
    16081 tcagctattt gggcagctgt caatgccaaa cgatagagtt gtccatctct atcgtcgtgc
    16141 actttatcaa agcttttcta gtttcggtat gaatcgacag ggacaggtcg tcggcagcaa
    16201 ttggggcagc tatcctgcta ctaatcgaat ttggaataag gatatgtatt acgcatcatt
    16261 gccattcacc atgtttgaac cggagttatg tcaaaaaacg gttctatggt ttgatcaata
    16321 tggcgttaag tttcctggtt caaaatttac aggaggtatc aatcattctc tgagtaattc
    16381 gttatccagt gcgttactgg ctagtcttta ttttgaacat actggggata caaaattctt
    16441 tgagcggtgt ccgcaggttc tactgaatgc ttcaagaatt attgaggaca tattagctca
    16501 acgtcggtca ggggaaccaa tgctttttcc atctgtttgg ttatcagacg cctttgcact
    16561 tggaaagtat cataccggca gcaatctttg tctttggaag gcctgtattg gacttgcaga
    16621 aatttttgaa gccctagaac aactgagtct tagcaagcga tacaaaaaca ttgcctgtaa
    16681 actaaaggaa tcaatttgta aacaaatgac tatcgatggt ccttttggtg agcaattttt
    16741 ggagggaatc ggtgatgagg agaaaacaac ctattcagtg aagcactatc aaaaaccgat
    16801 tcttgagcaa gggttaatct ttttaagtga tgtgattgtg gatggaaaaa ttaatttatt
    16861 gatgcatgat ggagaagaat cagacactac gttgatgcct ttttatcaat ttttagataa
    16921 taaagatcat ttatatcaga acaccatggc cttttctgct agttctttca atccaaccta
    16981 tagcgaggaa atcaaaggca tcacttgggg attagaatcc ggcgccactt ttcctggctt
    17041 tatcactgtt ttaatgagcg agctgcacaa ccctcaagct ttcgtaaccc gattagaaga
    17101 actaatttgt ttggcagatt tagacggttc gtggtggtgg tggccgtatc gcttagaggc
    17161 tcaacaagga gaagtcgtgc gagactttgg ctgcggcaaa tgcggctggg catcgggtct
    17221 ttttgtcagc ctaatgatca gtcaatattt cggattgaaa ttcaaaaaag gagtattgca
    17281 aattgatcct gttgataatt taacgttttc ttggaagaat ttgaagttcg gtcaagcctt
    17341 catttcgatt gaatgtacgc aatccgagtt gagtatttca aatttgtcag aaaagccatt
    17401 gaaaataagt gatacgaaaa aaattcttca cgtagaaaag ggaaaaacga ttcacattca
    17461 aaggagaaaa agatgagtat actaaaaaat attacccaat ttaaaaaaat ccagagtcga
    17521 gcaatcactc ccgaaaatcc aacaggcgag aaaggtaaag ctgcgatgtt gccaagcgag
    17581 ctaggaccaa gccgcaaagg tcgtggcgca attccgctga cttctggtga agaaactgtg
    17641 attgcggata ttactggaac gggaataatt cgtcatatgt ggatgacgat tcgtgatcga
    17701 acagaatttg gcagttttgt tttacgtgat ttaatcttaa gaatttattg ggatgggtcg
    17761 gatagccctg ctgttgaaag tcctttaggg gatttctttt gtaatgggtt tggagagcgt
    17821 tgcgaggtga actcgttgcc gatagtggtt aatcctacag ggggctttaa ttcctatttc
    17881 gaaatgcctt tccaaacatc cgctaaaatt acgatcgaga atttacatcc gaaggatatg
    17941 tcctcatttt tttatacgat aaattacgct ttggtggacg aattggaaga aaatagccta
    18001 tattttcacg caaattggca tcgggaacgc attaccaaat tacaggagga ttataagtta
    18061 attgatcgat taaagggtca aggctattac gtgggtactt accttgcgct tacctgccta
    18121 gaacgttatt ggtggggcga aggcgagttt aaattctaca ttgatgggga tagtgagtat
    18181 ccgaccgtaa cctcaacagg ttcagaagat tattttggcg gggcgtgggc ctttcatgag
    18241 tacgatgagt tcggacgtcc acatgcgaaa aactattcaa cagccttttt agggtatcct
    18301 taccaatcaa atagagatgc cacaagagat cgttttgaaa cgggaaaatt gaatgccgtt
    18361 catgcctttg gtgacgacgc aatgccacga catggtcttt atcgttggca tcttcaagac
    18421 cccatctgtt ttaccgaaga tttatcggtc actttacaac aaatcggcaa cgatgatgtt
    18481 cgcttatttg agcggcaaga cgatgttgct agtgtcgctt actggtattc aacagaagct
    18541 tcaggaaata ctaaacgtca tttcactgat cgagaacgct tgccgcgtta atgaagtgtt
    18601 tttcacatat tcgaagggag gaacctatga tgatatatgg ggcaattgaa gctggaggga
    18661 ctaaatttgt ctgtgcagtc ggtactgagg aattaacgat tttggaaaga gtcagttttc
    18721 ccacaacaac tccagttgaa acaatgaagc aggtcattga tttttttaaa ccttttaagg
    18781 aacaattggc aggcatcggg gtcggctgct ttggtccaat tgatattcaa tgcaaatcaa
    18841 cgagtttcgg tcatatcaca tcaacaccaa aacttgcttg gcaaaatttc aattttgtag
    18901 gaacgctaaa gcaatatttc aatattccga ttgaatggac aacagatgtc aatgcggctt
    18961 gctatggtga atatgttgct ggttctggca aagggttagc aggtgttgca tacttcactg
    19021 tgggaacagg tatcggaggt ggtgccttgt tcaatgggac tttcgtggaa gggttcagtc
    19081 atccggaaat ggggcatatg ttgatcaagc gacatccaaa ggacaatttt tcaggaaact
    19141 gtcccttcca ccaagattgt ttagagggga tggcagcagg accagcgatt gaaaaacggt
    19201 tgggtgtaaa aggccagaat ttattggctg acgattcgtt ttggcagatt gaagcctttt
    19261 accttgccca atgtgcatac aatacaacac tgatgttttc gccagataga attatttttg
    19321 gcggtggtgt aatgaaacaa gaacacatga agaaaaaagt tcaggataaa tttgttgagc
    19381 ttataaatgg atatgttgaa ataccaccaa ttgatagcta cattattacg cccgaattag
    19441 gtgataatgc cggaataatc ggcggattag cattggccag aaaagcggta agaaataagc
    19501 aaccataatt aatgaatgtt tcattgatac tcatgtcaaa agtatttaac cgagtaatca
    19561 aatgatcaaa tgaagttgga taaagcgaaa atgaattcaa ataaaaaggc tctaagtcaa
    19621 atcgtgttgt tttcattaaa tcactgaaag gaaatgcttc tgcgtttcct tttttctttt
    19681 tgttgtgcgc tgtatacctt gaggatattc actggtggcg tacacttaaa tagtaatata
    19741 ttgtgttact ttatgtctgt ggtaatagtt atagttgctt accaatgtat gttcattttt
    19801 cgaaatgttg acttcataat ccttggctta atgtttgaat ttttttggtg atagattttt
    19861 tcagccgtat aaaataaatc gtaattctat tatagagagt tgtgattata aaaagatagc
    19921 aagaaaaaaa aagaaagttt ctatacattt taaatatttt attcctattt ctattgctaa
    19981 aa
//
LOCUS       DAZKNH010000049        19394 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000049 DAZKNH010000000
VERSION     DAZKNH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.049"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..1380)
                     /locus_tag="ACG8MG_001617"
     CDS             complement(16..1380)
                     /locus_tag="ACG8MG_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0375662.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1377..2936)
                     /locus_tag="ACG8MG_001618"
     CDS             complement(1377..2936)
                     /locus_tag="ACG8MG_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0375663.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(2992..5472)
                     /locus_tag="ACG8MG_001619"
     CDS             complement(2992..5472)
                     /locus_tag="ACG8MG_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375664.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5456..5791)
                     /locus_tag="ACG8MG_001620"
     CDS             complement(5456..5791)
                     /locus_tag="ACG8MG_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375665.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5805..6839)
                     /locus_tag="ACG8MG_001621"
     CDS             complement(5805..6839)
                     /locus_tag="ACG8MG_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0375666.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6929..7609)
                     /locus_tag="ACG8MG_001622"
     CDS             complement(6929..7609)
                     /locus_tag="ACG8MG_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0375667.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(7779..8738)
                     /locus_tag="ACG8MG_001623"
     CDS             complement(7779..8738)
                     /locus_tag="ACG8MG_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0375668.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            complement(8959..10062)
                     /locus_tag="ACG8MG_001624"
     CDS             complement(8959..10062)
                     /locus_tag="ACG8MG_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFQ0375669.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(10284..10754)
                     /locus_tag="ACG8MG_001625"
     CDS             complement(10284..10754)
                     /locus_tag="ACG8MG_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFQ0375670.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            11011..12330
                     /locus_tag="ACG8MG_001626"
     CDS             11011..12330
                     /locus_tag="ACG8MG_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFQ0375671.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(12539..13477)
                     /locus_tag="ACG8MG_001627"
                     /pseudo
     CDS             complement(12539..13477)
                     /locus_tag="ACG8MG_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(13477..14094)
                     /locus_tag="ACG8MG_001628"
     CDS             complement(13477..14094)
                     /locus_tag="ACG8MG_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0375672.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(14108..14278)
                     /locus_tag="ACG8MG_001629"
     CDS             complement(14108..14278)
                     /locus_tag="ACG8MG_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375673.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(14626..16116)
                     /locus_tag="ACG8MG_001630"
     CDS             complement(14626..16116)
                     /locus_tag="ACG8MG_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0375674.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(16159..16458)
                     /locus_tag="ACG8MG_001631"
     CDS             complement(16159..16458)
                     /locus_tag="ACG8MG_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375675.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(16824..17108)
                     /locus_tag="ACG8MG_001632"
     CDS             complement(16824..17108)
                     /locus_tag="ACG8MG_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0375676.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(17627..18112)
                     /gene="vanZ-A"
                     /locus_tag="ACG8MG_001633"
     CDS             complement(17627..18112)
                     /gene="vanZ-A"
                     /locus_tag="ACG8MG_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0375677.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(18265..19176)
                     /locus_tag="ACG8MG_001634"
     CDS             complement(18265..19176)
                     /locus_tag="ACG8MG_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0375678.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         5610 a         3860 c         3255 g         6669 t
ORIGIN      
        1 agaccgtgat atttctcaca gtcttttatc tactctattg ctatttgctc tcttagctag
       61 tactttaaaa gctaactcat tgaggtagtg aaaacgagag gtgtattcag acagaaatcc
      121 aaaatcctca actgctagct gtggaatcgt agttttgtgt ttcacgatat ccgcaagcag
      181 gttcaaatct gataataaga attcttggct tatcagctgt ccgccaatta tttctaatgt
      241 atccggatgg tagatcagct ttgcatttac tgttttgttt tcttcgtcgt attgtggaaa
      301 tagatcaaat tctcctgaac acgaaccggt attttgttcc aagaatggcg cttcatcttc
      361 tgtcagccct acagttccta aaaagtagtc tataatgact gctgctgtta tcttttgtct
      421 gaggtcgtaa gcggttggct gaccactcaa agttagcgct actgctcttg ccatatgtac
      481 agctcgagta actaaaggta aaaagagatg ccgtttattt ttgtcaaaac tgactgggac
      541 gatatctcca atcgcataga tgtccggatc acttgtctgc agatattcat tgactatcac
      601 agtctcatcc tcatacaaag ccactttttc ttttaacaat gtcgtattag gacgagtttg
      661 ggcagaaaaa atgaccatat ctgcctttat tgtttgagac agaagttgta cggatactac
      721 tttctcttct gattttttcc aatcgatcaa gaattcattt ggataaaaat ttatttgctt
      781 agtttgcagc tgattttgaa aactgttgat catatcctta tcgaaatatc ttgataaaac
      841 attcggcata taatcaacta gatgaataat gtatccttta tttctcaaag catctgctaa
      901 ttccaatcca atgtaaccag caccgaagat cactacttct tttgcttctt ttaaagcggc
      961 aagtacttgc ttagattgca ctagattttt ataggtgtag acattttcag cttcaatttg
     1021 tttatacaag gtgacaggag aggaacctgt cgctacgatt aatttatcat aggttatggt
     1081 ctcgtttttt ccttctttgg atgcgaggat gagctgatgt ttgtctggat caagttcagt
     1141 tactctccat tcggtgatca gttttgcccc actttcttga agagtccgca tgttatgggc
     1201 aacttcagct aaactacgta tctttccttt taaataaagg ttgattccat ttgaaacata
     1261 gcctagtcca tctttatgat cttcatcgat cagtaaaact tctgctttcg gttgaagttt
     1321 tcttagattt agggcagctg tgatgccttc atgggaagca ccaatgatca cgattttcat
     1381 gttttattca tccttaaatt caatttttct aaataacatt cttttaaaaa ttggttcaaa
     1441 taggggaaat cataattata ttcgttgcta gtgaatacaa aaactttagc ggacttctgt
     1501 gacgggtact ctttcaattg gctagtcaaa atgatatcat actgcttttc tttctcgtat
     1561 tcgactaaat catatttgat cgtattcagc aattgttcct ggatgatttc tttcacgata
     1621 tccgccatta gcctttcata aggaagatca taagcaattt gcagacgtaa ttctaactgc
     1681 ttgatcagcg tccgtaaaat agatgcatag cgagcaaata attcaaagcg ggcattttcc
     1741 tcgtctactt cagtaaaatc aacaatagca aatgtttgtc tgaccaatct ttcacataaa
     1801 accaggaatt gtttatcttc tacttcagtt aaccgctgtt ttaatccttc ttggccaaaa
     1861 atattcatcc atcctctaaa ataggcaaaa tgagcgtgat tttgcatcac tgtcaacgca
     1921 atcttttgcg aaatctcttc tggaaaaaca taaaaagaga aatattcttc tatcgattgt
     1981 ttaaacgttc gattcaattg agctactacg tcgtctttgt atttttcgct tatcaaaaac
     2041 tcctggacat gcgagttatg tgtatctaat gtaaagttac tgatcatgaa aagataaaaa
     2101 accaacgttt cattttgatt ccaaggaaag gcatattgaa agaagtaacg ggtcaaaaat
     2161 gtatggaggt ccaataaaaa ttgtgtcgtt tgaaacaatc caacaggaag attgctataa
     2221 tctggtttgg attttttcgt acagcgcatt cgcttcttca taatggaaag ccatatttca
     2281 aatttcaatc gtccttcttc tgtaatagca aaactaaact ctttttgcag cagatctaaa
     2341 aaagtgtttc ctgataaatc actgtattct tttttgtttt caagataggg acgtccaaac
     2401 caaaataatt ggaaataaaa ataactaatt tgactttctg gaccggtcag ccgcccgctt
     2461 tttattttca gatgaaactc atttaaagaa tcatttaatt ccttcactct gcgagacaaa
     2521 gtagccgaac tgatattaag ttccatgaca agtttctgcc gatcaaatga gccattttcc
     2581 aacagataaa gaagtatctg atattttgta gaatagcgca agtaacaatg ataataaaca
     2641 tcgactgctg ctatatttcc atgtctgatt aataaaatat tcccttcttg gataacaatt
     2701 tccaattgtt tgatattttc caagttagct tgtaaataaa acagatcttc tttcaaggta
     2761 gcccgatcaa ctgataagcg ggtaagtacg ttattaacgg aaacctctcc tttttcctct
     2821 aataaaagtt ggaaaagttt gatttgccgt tgttcatttt tttcaagcaa actgaaaagt
     2881 tccaagtcca ctccttcaag catttctgat tttaacagca gaagtttcct tctcatcctt
     2941 gaaaaagagg atctgtgaca gttatcacag atccatacaa attctaatca cttagctggt
     3001 tggcccagct gtcagttccc atgtgaccat gccttgatac gttgcgccag gttgagcatt
     3061 cgtactgcta tttacctcta agagtaaacc atcggtatcg ggaaagtcaa cgagaacctc
     3121 tcctttagct gttccatcac tctcataaat caacgtgttt tgaccttgct gaatcaattg
     3181 ctttgatcca gaatgattga agtataattt tgttccgctt aattcttgac cttgatcatt
     3241 cttaaataaa gaagtcaatg tacctgtcag ttgccaagga gacgtgcttg gaagccgagt
     3301 atcccgcaca tcaaaagaaa aatcgccttt tgattcataa taacttgtct tcaacggttt
     3361 ggggagatcg ttaccaaatg taaaatcatc tggtacatta actaatgtta attcttgttt
     3421 ggggacaaga attgttttag cagatgtttg gacaactggg ttaggaagct tagcaccatc
     3481 tgctaatgta atattgctgg aaagtacagc tgcctttgtc agtatgctga cggattgtaa
     3541 agaagtatct acagtattta attgatagtt gtaagtaaga tgaatggtat ctcctatttt
     3601 gctcaataga tctgctggga cagaaactcc ttgttgaatg ccggtatctg cgatagttgt
     3661 accatttaat tttaaggaac cattgatata agtagtattt tcaggaagtg agatcgtgat
     3721 ttttttgtcg gttatcgcat ccccagattt tgttaacgtg aatgtcacat tttcttgaat
     3781 cgtatctcct gatagagcag acaggggatc tcctgtctca ggttgtctgc taaggttttt
     3841 tagctgacta tctgcactca cagatggaaa attagccact gttatttgag gaagagtggt
     3901 gattccagct tgttcagact gccatttgta cgcaaaagac ggatacatgt tagtaagtac
     3961 atcgtccacc aagcgataat caaaataata ttgccctgaa gccaatgcag gtagtgtaga
     4021 agtagctgta tttacacccc cgttatttga ttggatcgtt tggaaaagtt gatctgtcca
     4081 tcctgtcgct aatttttggg ttcctttcga ataaagcctg taatggatcg aacccttttt
     4141 tagttcagtt gctgaccaat tggccgtgac ttggtacgat gcagaagaaa ggagctttcc
     4201 ccactgacta tcggctgtta agtttgttgg taaaaccgtt ccgtctagct tgtactgctc
     4261 caataccttt aaagcaacag gagaaagcaa tccgccttgc gcatctttcc cactgtacac
     4321 gatattggct gtctgattca atccacttgt gcttgctgga gtatgagtca gtattgctcc
     4381 ttgtgtaacg ttttttcctg taaaatagtt gactacgcga tcaaagcgac taaatggcaa
     4441 ctgatcaaaa cgtccttcag tcagccccat cgtcaatgtc cgactattgg tagagcctgg
     4501 aaggttctgg ttatttccat ccacgtattc tgtggtatca gcaaatgtat tatattttcc
     4561 tgttgaatca cttggtgaga tgtaactatt ccacgtattt atctctgcac ttgcagaaaa
     4621 tgcatatgct cctggattca agtttccgcc agttaattta ggataaaatc tacctgcagc
     4681 tggagctaaa atcgaattga acatacgatc ttggataata ttattcgaat cataaaaatc
     4741 aacagaaaaa acggcctttt tagacataga aggttgtaat ttacccttgg tcaaaatttt
     4801 gtttggactc gtatcatatt tatctccgtt ttctgtcatc ttcgcgacat tagctgtaac
     4861 atcaaattga agcgggatat cgtccacgtt gaattcgcct cgcgtaataa tagcaataat
     4921 atctgtcaat agagtatttg tatcccacga ccccatccca aaacgcaagg tatggcttcc
     4981 tgctgataat aaacttccgc tagtaacctt gatgggggca ccggcaatat acaagtaggc
     5041 gttgttccag tcaattgctt ttaatacatc actgcttgat acatgttccg gcaaattgat
     5101 gatcgccaca aatgcaaatc cttgtgcagt gatattcacc aaatcaagta catttgttag
     5161 tttcacatgg ctttcccaat gaaaacggac cgtgccacct ttccaaggat catttgaacc
     5221 atcgttcaaa taagtagcag tcaatttagg caattggtta gccccagtta gttggacaag
     5281 cttcccgcca ttgactgcac tacgtccacc atttggaggt acgatatttt catacgtgta
     5341 tggatcataa gaaatggcag gaggcgttgc tccaacagct tctgccgaag gtgaaaagat
     5401 aaatatactt aaaacaaaaa gcgaagtaaa aaagaaggag cgaaaaaatg aaaatttatt
     5461 ttgtagcaac atgttcatcc tccttttcct ttcttctcca taacaacaac cacgcaagaa
     5521 gccataattc tataccaatg atcaaataaa gaaaacggat cttttgttca ttcgtctccg
     5581 ggaatacgcc atgttgatta gattctttat aaatcggtag ctgtgtctgc tgttttgatg
     5641 cgccattgac tgaaccactt ggatgcggaa ttggacttag atcataaaat gtaatcgtac
     5701 ctgaactttt ggtatctgct tggactggct cttgccagct gaagaacagc aaacttgcaa
     5761 taatccctcc caataagatc gatgttttca acgaaatcac ctcatcatgt tgtttctcct
     5821 tttttctttt tcttgtggac ccacaaaaac agcaaaatca accataacac tagaactgca
     5881 gcaaccaatc ctgcataaag ccatgggaaa gattgttctt gtactgcaaa aacggaattt
     5941 tcatttactt tttgtgcttt ttcttttttg atcgtaaagt tttgtgtaaa tcgccattca
     6001 ttactgtcgc ctgattttgc tatgatttct gcgcggtaat cgccggcttt tgctttatca
     6061 ccagctaaat caagattcaa gtgaaaattc gaatttggtg caactaaaaa tgcattagac
     6121 gtattttcaa tataaagctg attttcataa aagatcttgc tcgtaaatat caaattgctg
     6181 ataatcctag gctgaggatt ttggaaacga agctgaacag ttggctttgc gttgacctcg
     6241 tccaaatctc ctgataaaag tttcagatcc ggttctatcg tctcttgact ttcttgtaaa
     6301 acgactgcga gattataagc aatacgattt ttgatttgtg ctccttctgt agcattttct
     6361 gtttcagctt cttctttagg tgaaacatga atacccgcta atacacgtcc cttaaagctt
     6421 tttgctggga ttttcacttc tataggtaca gttgtttctg catgtgctga tacattgatt
     6481 tcctttttcg caatgagcaa aacgtcacgg acatcaaacg gcaatgtctc atctaacgtt
     6541 gggttttttt gactataatc gatcgttcca ccatcactcg ttaccgccgg attgacagag
     6601 acctcaaatg ttcgttcttc atctgaagaa ttgctgataa ttacttctag tgtctgattt
     6661 tgttccggtg ccactaataa atcaaaataa ccgattgctt tatctatttg gttctctgga
     6721 aataccggtt tgattccaaa gtctcctgct gaatcctttg cttggctgat tgacggaaaa
     6781 atgaaaaata gacttaaaat agaaaaaaac gaaacaaaca gtcgtttact ttgattcatt
     6841 gtccaccaca tcctttcaac aacaactgcg gtaaaaaatc cttcacctat agtattttca
     6901 ccgcagtttg ttgaatttat ttcatactct atgctactgg gccagccacg aggttccaag
     6961 tcaaatcagc cgtgaatgtt ccagctgctg ctttcggcac tttcagtgtg atatttgctg
     7021 gatcataaac agaaagccat gttcccagac cttgatcttt tgctgctgta aatattggtg
     7081 tcgctgcact tgctttactc aacccagtta cagtttgact agttggtcca gtggattcgt
     7141 tatctgacgt acttttaaca gtggatgttc caaaatctaa ttcagcgcct tgcaaaactt
     7201 gtgaaccgtt tacaaaatct gaaaccgaaa ctgtcacgtt ccacccttgt gcttggccat
     7261 ctgcacctac acctcgacga tctgatactt ggagataagg tgtatcgttt tttgaagcgt
     7321 ctacttgata cgtttttgtt ccgctttcaa tctcgtgtgt accaaatggc aattcgggaa
     7381 ctacatccag tgttaaagga cctgtctggc cagttgctgg attttccgga tctgctggct
     7441 ctactggttt tgaaggatca gtagggttaa gaggctccac ggtatttgtt ggttcagtaa
     7501 aggtaatatc tccttttgaa gtcgcttgac cctcagcaaa agctggtgtt gccaagccgc
     7561 ctagaagaag tgttgaaagt aataatgtcc caatcatgtt ctttttcata ataaatcact
     7621 cctttttctt agtgacctaa gtatagtaga gacagtatgc aaaaaatcct catgaattca
     7681 tccgcttact tgaaattatg accctttttg tataaaagag aaaaaaacaa taaaatcgtt
     7741 tacatgaata ttcttccttt tttattaagt ttcgtttgtc aaattaagtt agacaaatca
     7801 tcttttgtga cgtaactcaa aaatacttcc attggtgtcc gatagtgtaa tgattttcta
     7861 ggaatatgat ttcgtttcga tgccaccgat tggataaagg tttcagatac accattaaaa
     7921 tccatagctt tatacaaacc atctttacgt aataagccat ttgaatgttc attcaatcct
     7981 cgttgagaag gtgttcctgg atccgcaaaa taaattgaaa tatcgttggt attactaatc
     8041 tttttccaat ttgagaattc ttttccacaa tcaaatgtga tggatttaaa taagtttttt
     8101 gggacacaac gaaaccattg attcagccta ttttcaatgt ctttagcctg tctgccttcg
     8161 ggtttcaagg taatgatgac tttagatagc cgttcaacta atgtgatgac cgcgctttta
     8221 tgttttccac ccacgatagt gtcaccttcc aggtgaccaa attcatgatc aaactgggta
     8281 tagtctgtct ttctgtctcg gattgaccgg cgaaaccctt gttttcctct tttttcttta
     8341 tgaccgtttg gttttctttt tcccttcatt ggtaaatcgt gagtgtcaaa ggttttatct
     8401 ttaaacattc gatacagtgt gcgtaccgaa caagcaatag gaaactctgc tcgaccaata
     8461 aggacatcag gagcccaccc ttgaacaacc tttttttgaa tgtattcctg ttgctcatca
     8521 ggtaaaacaa tcggatgtct tccgcagtta cgtttgtttt tcttatattg tttatagtag
     8581 ctcattgcgc ttccaccaga tttaagaaaa cgatagacct tataaatagt ttgtcgagaa
     8641 cgttgtaaca gattggctac ttttgaaaca ggttgtgatt gattgaaata agcttctatc
     8701 attacgagtt cagttggtgt aagatggtta taggtcattt gtactcactc cttataattc
     8761 tttcgtcgga actattttga gtgtagcaca aatgactttt tttagttgtc tagcttaatt
     8821 ttacaatcgg cgaagtatga aaaaatattt caattttatt ttttcgtttc aaattattct
     8881 attagtgaca tccttgaact ataaaaatag gagtacggca tcatctgccg cactcctatt
     8941 tttttcgtca tttgtttctt aaataaaacg attctcacgc aattttattt ttttgatatc
     9001 ttctactgtt tgtgttccag ctaattgcat gaccatttcc aattctgttt taaagaaatc
     9061 aaacacttga tggactcctg tacttcctcc taaagacaat ccataaataa cgggtctgcc
     9121 aatagcaaca agatctgcac ctgatgcgat tgctttgaaa acatgctgtc cgcgtcgtac
     9181 accgctgtca aacacgattg gtacacgttt atctactgct tctgccacat attgcaacga
     9241 atcaaacgct gctggaccac catctagttg acggccacca tgattagata cccagattcc
     9301 gccagcacca gattctaatg aacgatagac atcttcttct gactggacac ctttcacata
     9361 gactggcaaa tcagaatgag cagcaataaa ttctacatct tttggactaa gcttctgttt
     9421 ggaagatttg tacacagcat ccatcgtttg gcctactcct gattgataag cctgaacgat
     9481 cggcattggc agtgggaagg taaacccatt tctgcggtct gtttcacggt ttccgccaac
     9541 tgttgcatcc gctgtcaata cgattgcttt tgctccatta cgcttggcca cttcaagaat
     9601 atccaaattg atcccatcat ctttgctcat ataaaattga aaccattgcg gtgctttctc
     9661 cccgcctgct tctcttatct cttctaatgt acaagaagcg taagaacttg ctgtatagat
     9721 cgtcccaaaa tctgctacac cttttgcaga agctttctcc gccttcacat gagctaagcc
     9781 atgagcagca actggtgcca tgatgattgg tgcagtcagc gtttctttgt caaaatgtgt
     9841 agttgtatcg ggtaattcta catctcttaa tacatgagga ataatcagct gatgattgaa
     9901 tgcccgttca ttttcttgat aagtaaataa gtcgccagca ccactgctga tgtaaccata
     9961 accaccagct ggaatcactt gtgaagcggc tatttctaaa tcttccatat tgataaaatc
    10021 cactattcct tcatttgtac ctgcttgata cgttttttcc atttcaatgc cttccctcta
    10081 taaaaaattg aatgaataat atgatagcgt ttacacactt tatattactc ttccgtattg
    10141 atacaggcaa ataatctgtt tacatttcag tcattccagt tgttactact ctgaaagaaa
    10201 tgtttagaac acaaatagat aaaaaacgat gggattttga caaaagtcgt gtcaaagtcc
    10261 cttttacgaa atactgtgga tgcctattga aataagtgcg ggttgtttct ttgtatttca
    10321 atcgtttctt gccattcttc gtttaatttt ggattaccgt tttcttcctt gaacgcataa
    10381 ggtcccaatc caaaaagctc agttagtatt tgattcacga ttccatatag ctcggtgtga
    10441 tcggacacat gattattgaa gatcatctca ttatcaccac gtttgaatct cacattcggg
    10501 ctctcatgat gaacctctat gcagttttcc ccttttttta actgacggat ttctgaaaac
    10561 atctcataaa tatcttctcc aataaaacct tcagcaagcg ccagactgtc cacaagaccg
    10621 cctactgtca actcagagat tatcgctttg ttggcaccaa gctttaatgt atagcttgtt
    10681 gcttctacta cgccgcatgc attgcaacca ccttctgata gttcgctttt tctgatcaat
    10741 tgaacttctt tcattttctg ttcctcctct tagttttcta cttatgtatt ttttatcacc
    10801 tgacgtaagt gtattttatc gtcacgatca cgtgatttat tcttcagaga ttctacctga
    10861 ataattcata cttttaatta aaaccatgtg aaactgcctc acggcagctt acaattactt
    10921 tttcatcttc acccgttaag tgatgaaaaa agaacccctt tctgctatgg ttaaagtgac
    10981 taaaccaacc aaaagaaagg agttcttttc atgactagct tacacaaaaa ccaagtaaaa
    11041 ttcaattcaa atatcactat ttctcataca ggtggtcaat tatcgagtga ttcgggtctc
    11101 gtcctagtga aagagctgat gaacatcttt ggtttttctg aactggctaa gcaacacatt
    11161 cacattgaag acgaacgagc gtattttact catgacaact tatccatact tgagcagttc
    11221 attatgcaat taattgctgg ttactcagct gattctgcag ctaatctcct gagacaagat
    11281 cctgtgttta aagctgtttt agataggaaa gaactcgctt ctcaatcttc actttcccga
    11341 tttttagatc gactatctga ggagaatatc catgaacttc aagctttgaa ccaagaactt
    11401 atcgataaag cacgcctcat ccgtaatgat acggaattaa tcattgattt agattcgacc
    11461 cattcagata catttggtca ccaagaacaa acgaattata atacacacta ccaaacctat
    11521 ggttaccatc cattggtagc ttttgacgga ttgactggtg acttcttaaa agctgaacta
    11581 cgttcaggaa atcaatatac atcaaaaggc gtaaaggagt ttctcacacc tttattagag
    11641 cactataatc actctctacc aaacactgac atcttggttc gtggagacag cgggttcgct
    11701 acacctggtg tgtatgattc gtgtgaatca aaaaagagtc attatgttat tcgactgaag
    11761 aataatcgta gactaggtca aatagctgag aaatcagtac tttatggcga taatcaaaag
    11821 tgggaagaac gagaagttca gtacttctcg accacttatc aagcacaatc ctggtcacaa
    11881 agtcgtcgcg tgtgtatacg ctcaacacgt gaagcgggcg aactactctt tcgacatgaa
    11941 tttatcgtga cgaatctatc agaaaatgtt tctcctgata ccatcttttc tatctatgcc
    12001 aaacgtggca caatggagaa cttcattaaa gaagcgaaag ctggctttta ctttgacaaa
    12061 acagacagtc ctcgcttttt ggagaatcat gtccgaatga tgctcagctt gatagcttac
    12121 aacttagtca acttcttaag aacgattggc tttgaagaag tccaaaaggg aatgaccatt
    12181 cattctattc gattgaagtt tctgaaagtt gctgggaaat tagtccaaac gggtagacga
    12241 gtctatctca aattatctag ttatcatgtg tatcagaatg aattctacag ggtctttgct
    12301 cgcctgaggc gagccagtca atggatctaa tccaacaatc taacgatttt tttactggga
    12361 agttatccag ggagaagtat gctcaaaatg ccttacaccc aaaatttatt cctcaatatt
    12421 tttgaacagt gaatgtttga agtaactttt tagtcaaaat cagttgatag ggatattagt
    12481 gatacatatt tcaataaaat gtgccaaaga attaaagcta tgaattattc aggaaagaaa
    12541 tagcttttaa agtttggctc gctgtcgctt tataaagctg attgactttt gattgcaaac
    12601 tacttaaaga aaacaaactc ggactattcg ttttcttctc tttggtttga acatcagcaa
    12661 ttatcccctc ttgattgcct attttagctt gtttagaaga aacaaaagct aaaagctcct
    12721 cttgggtttt aaaacgctgt gtggggctta gaacgccctt aaacgaccct tggtttactt
    12781 ttatactagc ttccacctcg aaaaaaggtt cttttttaaa attctctatg gcttcctggc
    12841 gctgaaaaat aaggtataag gtgggcgttt gaacacgtcc tagtgaaaat gtaccttgta
    12901 cgcccttctg ttgtaaattt aacgtataca aagggcttgc gttcatgccg atcaaccaat
    12961 cggcaatttg gcgtgtttgc gcttcttgat aaaagggata gtaattcatt ccaggttgca
    13021 aattttgaaa accgcttcgg attacatctt tttctaagct attgatccat agtcttttaa
    13081 atgttttatc ttttgaaaag gcatttgctt tatggataat cgaccaggcg atattttcac
    13141 cttctctgtc gctatctgtt gcaacaataa ttgtatttgc ctttttgaga agttctgcaa
    13201 caattttaaa ctgctttccc ttatcttttg caacttcaaa atcgtatcga tcaggaaaaa
    13261 tcggcaaaga ttcaagtttc caattttgcc acttttcgtc ataatgacct ggttctgcta
    13321 attccactaa atgcccaaaa ccaaaggtga taaacgtttc atctgtaaat agtgggtctt
    13381 tgatctcaaa ataaccgtct tttttggtgc tttgttttaa agcacttgcg taggctaatg
    13441 cctggcttgg tttttcagct aaaataaccg tactcattaa ctatccctct tttcattgtt
    13501 ttttctttga tcgactgtca cgttatatct tgctcgatac cttctaaacg ttcggcgatt
    13561 gattccagtt tgttcttcaa cttctttatc ggataaacca ttcaaaaaca aatcgaaagc
    13621 atgctttaaa cgtggatcat tttctttaaa tttatgctga cgacctttga atttgccttt
    13681 tttcttagca atttcgattc cttgtgcctg acgttcctta attttttttc gttctgattc
    13741 tgcttgatac ttgtacaatt caatgacaag gctattaatc aaacgcctta aattttcatc
    13801 ttcaatacca ttcattgagg gtaaatttaa gacttccagg gttgccccct taatttgaat
    13861 ttgattcatc aattctgtta attctttatt atttcgtcct aatcgatcta attcagtaac
    13921 aataacaata tccccttcac gaatatagtt aagcatagct tgtaattgtg ggcgttcgac
    13981 cgattgaccg cttaatttgt ctgaaaagac cttagaaacg ccctgtaacg cttgtaattg
    14041 ccgatctaag ttctgttctt tgctactgac acgtgcataa ccaattttag ccattttcaa
    14101 ccaacctcta aaattctctc ggttgcaata accaatcagc aatatctact ttttcaattt
    14161 caaattgctt atcagaaatt gtcttttcgt aagcgataaa atcttgcgca tattgttgct
    14221 cattaaaaat agccaccact tcgtcatttt ctaaaactcg ataaataaat tttttcattt
    14281 tactcctcct attatgccca acttaaatga cctattcacc aagtcaatta tactgctaaa
    14341 atcatattag gacaaatagg tatactctaa tgacctataa aagatagaaa attaaaaaat
    14401 caagtgttcg cttcgctctc actgcccctc gacgttttag tagcctttcc ctcacttcgt
    14461 tcagtccaag ccaactaaaa gttttcgggc tactctctcc ttctccccct aataattaat
    14521 taaaatctta ctctgtatat ttctgctaat cattcgctaa acagcaaaga aaaaacaaac
    14581 acgtatcata gatataaatg taatggcata gtgcgggttt tattttcagc ctgtatcata
    14641 gctaaacaaa tcgagttgtg tgtccgtttt agggcgttct gctagcttgt ttaaagtctc
    14701 ttgaatgaat gtatgctcta agtcaaaaga atttgtcagc gcctttatat agctttcttt
    14761 ttcttctttt tttactttaa tgatcgatag caacaatgat ttaacactag caagttgaat
    14821 gccaccattt cttcctggtt taatcttaaa gaaaatttcc tgattcgcct tcagtacctt
    14881 cagcaattta tctaatgtcc gttcaggaat gcctagcact tctctaatct cttttttggt
    14941 cgtcactaaa taaggcttgt atacatcgct tttttcgcta atataagcca ttaaatcttc
    15001 tttccattct gacaaatgaa cacgttgacg ttcgcttctt tttttcttga atttaaacca
    15061 cccttgacgg acaaataaat ctttactggt taaatcactt gatacccaag ctttgcaaag
    15121 aatggtaatg tattccctat tagccccttg atagttttct gaataggcac ttctaacaat
    15181 tttgattact tctttttctt ctaagggttg atctaatcga ttattaaact caaacatatt
    15241 atattcgcac gtttcgattg aatagcctga actaaagtag gctaaagaga gggtaaacat
    15301 gacgttatta cgccctatta aacccttttc tcctgaaaat ttcgtttcgt gcaataagag
    15361 attaaaccag ggttcatcta cttgtttttt gccttctgta ccgcttaaaa ccgttagact
    15421 tgaacgagta aagcccttat tatctgtttg tttgaaagac caatcttgcc attctttgaa
    15481 agaataacgg taattaggat caaaaaattc tacattgtcc gttcttggta tgcgagcaat
    15541 accaaaatga ttacacgtta gatcaactgg caaagacttt ccaaaatatt ctcggatatt
    15601 ttgcgaaatt attttggctg ctttgacaga tttaaattct gattttgaag tcacatagac
    15661 tggcgtttct aaaacaaaat atgcttgata acctttatca gatttgataa tcatagtagg
    15721 cataaaacct aaatcaatag cggttgttaa aatatcgctt gctgaaatag tttcttttgc
    15781 cgtgtgaata tcaaaatcaa taaagaaggt attgatttgt cttaaattgt tttcagaatg
    15841 tcctttcgtg tatgaacggt tttcgtctgc atacgttcca taacgataaa cgttgggtgt
    15901 ccaatgtgta aatgtatctt gattttcttg aatcgcttcc tcggaagtca gaacaacacc
    15961 acgaccgcca atcatgcttg attttgagcg atacgcaaaa atagcccctt tgcttttacc
    16021 tggcttggta gtgattgagc gaattttact atttttaaat ttgtacttta acaagccgtc
    16081 atgaagcaca gtttctacaa caaaagggat attcattcag ctgttctcct ttcctataaa
    16141 tcctataaaa taggttgttt aattaacttg gtttgctttt tcattcaact gtttcaatat
    16201 tgcatgtttt gaaaaagatt tttttccttt ataagtcaat ttttttccac taatcgaata
    16261 aattattttg ttattttcta ttaacttata tatataatct tccccctccg aagaaaaata
    16321 cttatctgat tttgtttcta agtagatatt tctcttttct aactctttct taaacgtttc
    16381 tagtgtatag atatttgcta attttcttat ctccaataaa ctatttttta tataagtttt
    16441 acattcatca tgattcatac aaactccacc ttctataaat gaatacaaaa aaagcaatca
    16501 aacgatttcc gattgattgc ttaacaattc ttaaattcag tagcttagat acttgaaaac
    16561 tctctgattt ccctatataa tgatagtacg gttatatacc gtcttcaaac aaagttaatt
    16621 aaataacttc ttacgaggga agagttcatc tgactaactg ataagcgttg gtttggcaat
    16681 cttatcgggc tatgcattta taaaatgtcg tcaaacattt tataaatgtg tcatggctct
    16741 tttttcgttt ctattcagtt cgttgtttcg ttatatctag tataccgctt ttaaaaaaaa
    16801 taagcaacga tttcgtgcat tattcacacg aagtcattgc ttttttcttc ttccatttct
    16861 aaatccaatg ttacttgttc tgattctgtt tctggttctg gttctgttgg ctcatttggg
    16921 attaaatcca ctactagcgt tgagttagtt aactttgcaa tttgttctag tgtttttatg
    16981 gttggatctg attttcctga ttctattcgt gaataatttg atctactcat ttctaattct
    17041 tgggagaatt tttcttttga tttggtacct ctcatctttt ttaagctttc tctaaatgct
    17101 agttccaagt ttctcccccc tctttttgtg taataaaatt cacgctttat ttaaattata
    17161 gcttttaata tcttaataaa caagtgatta atcacttgtg atttgtgatt attccaaaag
    17221 taggtaattt caccaacctt tttcaaaagt ttttatcgac gattattgga ctggatcgtg
    17281 tttcctaaag cgattctaag cgcatgttta attttaaagt gggtaattct ctttaaaacg
    17341 tcctacaaag attcaaaaaa agcatggctg ctagtggggg taccgtcagg aaaatgcgga
    17401 tttacaacgt taaggaaatt tattaaagag accaattcct cagcactaaa attgataatt
    17461 gggctttctt tgttactata gtgttaggta agctttttac tttatcaccg gttttcccct
    17521 cacttcacac ctacgggcga gtttccccac atcccgtttt ccatctaatc caattcattc
    17581 aatatcttta tgtgtatgaa agatcagatt gcttgattta gacatcttac acgtaattta
    17641 ttcttaaatg ggtacggtaa acgagcaata atacgagcaa aagtatacct acaaaaataa
    17701 taactctgtc taattttttt tgattcatat gcttattgct taaaccatat aatttcagtc
    17761 caagaaagcc tccaacagta tttgtaatta catctgttat gtctgtcgct ccaatagcga
    17821 agataaattg aattatttca aaagtaagac ttaaaaccag tacaaaagca aacttaggta
    17881 aaaatccgat ttctttaaaa ttgacattca aaagcaagcc aaatggaata aagattataa
    17941 cattatctat catctctctg aaattcccag tagcagtaaa tggagtcaag ttaagacttc
    18001 tttgatgata attaaatact gataaaatat tgtattgtaa tttgaataac actaaccaga
    18061 ttagtgtcac taaatataaa gctagcaatc ctctagataa tatttttccc aatataattt
    18121 cctctaattc ttttgtttaa gttgtatcat tagtatagca atgtcctagt taacgtgggt
    18181 tttaaattaa aaagctaacg ttaccccaaa agtgctagta caataatgct gttcattaat
    18241 ttcgttggtt tcattccgcc atccttacct ccttgaatta gtatgtgttg atccgggaaa
    18301 cactgtcaca attacaccgt ctatattgtt tcctgatatc tcataacgaa gattagtcgg
    18361 cacatgaatg gtggtatttt tagtaacagg ataataaaag atctcatatt tttccccatt
    18421 tacactaaca gaaatggttt tttcttcttt taggtaatcc atatattcct cgagaacgaa
    18481 attcttttct ttcataatcg cactatgtgg taaaccaaca tagcgaatat gccatggttc
    18541 atattgaatt cctgttaact ctgttttgtc ctctggataa cgtaaaatga acccgtattt
    18601 ccaagcattt tcttctatcc actttccttc aggggctcgt tccattttcg tcaagcttga
    18661 tcctacatct agtgataaac ctgaattatg ctcactataa cctgctggta aggcatactc
    18721 agcccccatt tcttggtaaa gcacactttg ctcatcaaag tctcgatagc cactattaat
    18781 aataaaatga ctaacgccac cctttacagc atcattgacc atctctgaaa atttttgtgc
    18841 tatttctttt gacatataaa tattactatc aagcaacccg tatccattta ttaattcgtc
    18901 atgtttagat aaattcacga tatctgactt cacactttct tggcgaacag gatatttact
    18961 attgattaat agcagatttc cttgataaac ctgttcttct gtaattgttt tctcttgggt
    19021 attttcagaa gtcccactat tttctaaatg ttctttggga ttttgatcat aattttgaaa
    19081 ttcgactttt tcctgagaaa acagtgcttc attaacgtag tcataaccta agtatattaa
    19141 gaataacaat aacaataaaa aaaacaactt cttcattttc agtctcctcc ttaactatat
    19201 agttggaagt atgaaatatt tctagaatct tccccttaaa cggcacaatc tcgtttgaga
    19261 ttgtgccgtt ttgccccgta aattacagtc aattatttcg ccctactgga tttactatta
    19321 tcaccaatgt agatgaattt tcataagtga tacccatgct gaatttaacg aggctgaact
    19381 gcctgttgac aatg
//
LOCUS       DAZKNH010000050        18703 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000050 DAZKNH010000000
VERSION     DAZKNH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.050"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..1000)
                     /locus_tag="ACG8MG_001635"
     CDS             complement(89..1000)
                     /locus_tag="ACG8MG_001635"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0375679.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1377..2015)
                     /locus_tag="ACG8MG_001636"
     CDS             complement(1377..2015)
                     /locus_tag="ACG8MG_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375680.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2019..4001)
                     /locus_tag="ACG8MG_001637"
                     /pseudo
     CDS             complement(2019..4001)
                     /locus_tag="ACG8MG_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4181..4846
                     /locus_tag="ACG8MG_001638"
     CDS             4181..4846
                     /locus_tag="ACG8MG_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0375681.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5243..7276)
                     /gene="brxL"
                     /locus_tag="ACG8MG_001639"
     CDS             complement(5243..7276)
                     /gene="brxL"
                     /locus_tag="ACG8MG_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFQ0375682.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7394..9916)
                     /gene="pglZ"
                     /locus_tag="ACG8MG_001640"
     CDS             complement(7394..9916)
                     /gene="pglZ"
                     /locus_tag="ACG8MG_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFQ0375683.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(10032..13664)
                     /gene="pglX"
                     /locus_tag="ACG8MG_001641"
     CDS             complement(10032..13664)
                     /gene="pglX"
                     /locus_tag="ACG8MG_001641"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFQ0375684.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(13728..17399)
                     /gene="brxC"
                     /locus_tag="ACG8MG_001642"
     CDS             complement(13728..17399)
                     /gene="brxC"
                     /locus_tag="ACG8MG_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFQ0375685.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(17424..17999)
                     /locus_tag="ACG8MG_001643"
     CDS             complement(17424..17999)
                     /locus_tag="ACG8MG_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFQ0375686.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(17999..18598)
                     /locus_tag="ACG8MG_001644"
     CDS             complement(17999..18598)
                     /locus_tag="ACG8MG_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFQ0375687.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         5766 a         3566 c         2618 g         6753 t
ORIGIN      
        1 ttaaactact gttgatttag ttctaataaa aagatttaga tgaacagttt tattgattta
       61 aatagttcat attttttatt tataccgctt atttactact tgctgtacta gaatctgaag
      121 tgaatgtact caaaacattt tcaaagtctt tgtctttgat ttttacatta gcgtctttca
      181 atacttcgcc aattacttta ttctggaagg tactgtcact taattttgtg tctgttgcaa
      241 tttctttcaa ttcttttttg tatttatcca tgtcgttgcc tttgttttgg tttttcacca
      301 ttttaaccac ataatattcc gtagtataag ttgaagcatt ggtagaagtg atcacatcag
      361 aaacttgacc atctttcaac ttaaaggctg cttctttgac ttctgccgga accgttgtag
      421 tagtggaatc aaatttcact tttccgccat cttcttttgt cgtagtatca gttgacttgt
      481 ctttggctaa tttagagaaa tcgtctccct tatcagcagc tttcttcaca tcttttgcat
      541 catcttcact tgctacttgg ataatttgag cttccacttc tggatggaaa gattcccaag
      601 ccgtttttaa atcttcatcg gtcaatttga tatgtttctt tagaccttct tgaaaggcta
      661 gactttgttt gatactgtct ttgaatgttt gttctgtgta acctgcagat ttcaattgtg
      721 aatcaaatga atcacccaat tgtttctttg tttgatcata ttgggaatcg atttctttgg
      781 ttgatacgtc atctccatat ttatcttcaa atacttgata aacgatcaac tgttgcacag
      841 cggaatctgc tccttgcatt tttttcaagc tatccattac ttcttgttcg ctgattgtac
      901 cgcctttata actaacggct gttttttcac cggcattcgt acagcctgac aataagccag
      961 tgcctagtac gatggcaagt gctaaaaatc tttttttcat ttatcaattc tcctactttt
     1021 cgttttttgc gaattgctaa aaacaaaggt gttcaagatg aaaatcatca agaacacctt
     1081 tactaatctg ctcgatacgt cctgaataga tataaggcta cacaatccag aaagttcgct
     1141 cagcagaata aatgtcaagt agcacatcac accttaatat actacttctt ttgttaccgt
     1201 ttttcaagtc tatcttgcgt tccgtattta acgtaactaa ttcttagaac aaatgttatc
     1261 gattttgttt ttcttcttcc gctgttggga tattcaaatc agacactttt atgttcgatt
     1321 ttccagacat ttttttgttg tttttattag aaaaagtttg ttttttcatt tgttttctaa
     1381 agcatgatct tcttgtttcc tattttggtc agatatggat catgcgtgac cacgataact
     1441 aacgtacctt gggaagcctg ttgcgataag atttcaacga cttgctcctt atttcgttta
     1501 tctaatgatc cagttggttc atcggctaaa atgattttgc tattcttcaa taagactctc
     1561 gcacaggcga ttctttgctt ctcaccacca gataatgtgt agataggcgt acttagcttc
     1621 ttgtttatat ttactttttt caatgcctca tactttgctg ttcgcttatc tgaaacattt
     1681 aaattcgaaa acttcaatcc aatatctaaa ttaaaatcga ttgtttcata ttcaatcaac
     1741 ccaaaatctt gaaaaagaaa agagagggca tttcttttta tcaagcgctt ctctcttcca
     1801 gttaaatgtt ttgtttcttg attaaataaa taaaattgcc ctgaactgaa ttgatcattc
     1861 aatcctaata tattcaataa agtacttttc cctgctccgc tctctccaac tacagtcaca
     1921 aaatccccag catccgcagt gaaacgatcc aaatgaagaa tcgttttgtt tcgaatgatt
     1981 tttttcgcac cttttatcac tatcaacggc tccaattgtt aacctccatt catcagcaat
     2041 aactgacttt tcttttctgt agacaaaata aaaacaactg aaagaataaa atcaatgaag
     2101 ctaaagaaaa aaatattaaa aagatattta ggctctcttt ctataagaaa aaggaagatt
     2161 acacttgtcg ttccccattt gatgaggttc atcatgaaga aatctttata cgtttcaaag
     2221 agcgaataac cacttgaacg aaagatagcg attttccgac aattgtagac aaaatatatg
     2281 cataacatga catagctcaa caagaagaaa gaaaagaaag tgagtaaaaa tatctgtatc
     2341 aagtaatgaa atgatctgct taacactttg acagctatct cttcagtctg agtatacgga
     2401 aatgataact gcaagttatc acgtaactga ctttcttcaa tcagctcaaa taacctttct
     2461 ttcgacaaag agcctatctt gatctttaac ggtggataga tttctccgct gagaatattt
     2521 ctttcccaat acgtactgtt tttcaatgtc agtatttcaa caaccggata atgttcgatc
     2581 caggggatag aaggaataaa tgtataaatg ggttgattgt tttttataaa tagaaatttc
     2641 ggttcttttt cactgatttc ttgataatat tctgttattt gcgccaggtc attttttaaa
     2701 tctgtatgtc tatatcgttc agggatcaat ataacagggt gcgtttcttt ttcggttatt
     2761 tggatcaacc ttccagagac atcaatcaaa ggaaatttct taagataatt cagatttatt
     2821 tttatccctc tgccaaactg ttcattttct tcattcaagt aatctgatac attgactaat
     2881 atacttccgt ttttattcaa agtttcatat aattcctgat cggctgctga tgcaaaacga
     2941 ttggaataaa taaaatccac gtggtttttc ccgatggtta ctggataaaa tacatgatag
     3001 tcctcaccag cgatttcttt tggtgtttca tgggaaacag ctaactgtga agaagaaaaa
     3061 acgatagttg ctaattcgac attggcaacg gtcaaaagaa gagaaaacaa caatccaaat
     3121 gtaaaaagaa tgtattggat atagttgaac gcttctctta acgaagcgat tttttcaact
     3181 aataaaagag aaatcaaaga gataccctat attccgctga acaagaaaac gtacatctgg
     3241 aacaaataaa gaaattgctc cttataatac ataaaaataa aaccaccaat gattcctgca
     3301 attatgaatg taataatgat tttctttttc cccaataaac accacaatac ttgatgtaat
     3361 gaaaagccat tattcaacaa taaactgatt tctttattca tcaataagaa aaaggatgat
     3421 aacaaaagga ataaaagaat caatccaata gttaaataca ctcgataatt atatacgtct
     3481 gtacttgaaa taaccgaata cgaatagcta ttttcaaagt cttcaaaaat attagtggca
     3541 taatcagtgt gaaatgcttg attgaatcgt tccacaaata gtaaaatgaa ccgttcatag
     3601 gcttcttttt ctctcgtcat caagaaaaat tcaccttcaa aatcttttat tttgatcgat
     3661 tcttcaatgg acgttacgcg aaaggaggta ttagaatagg gtaccttctc gtgagaagat
     3721 ttgacatccc cttgataata caatgtcatt tcaagctttg gctgtatctt gaagttccaa
     3781 gatgtatcta cttcattgga ttcatttact gtttttttca tataagaaag ctcagtttct
     3841 gatgagacat tttcaaatac catgattagc tgctcatatt cttcttgccc aataaaatgt
     3901 tgtggcaatc gaatcggagt ataagtatct gtttgatgga aaaattcatt tgtccgatag
     3961 agtacctgtt tatttataat aaccaaggca aaaccactca tgataccggt cactatgatt
     4021 gtgagtaaaa ctgtgaactt ttttttgatt atcaccactc tcccttactt aaatgattct
     4081 ttgataaaat cataccgttt ttcatcaaaa aaacgtatct atcaaatgta taggtcatgt
     4141 agaataaatc tatatactgt agaatcgaaa ggagaagaag atgaacatcg gagaaaaaat
     4201 acgaacttac cgcagaaatt gtaagcttac gcaaaaagaa ttaggcgaaa aaatcggtgt
     4261 ttccgataaa acaatctcta gttgggaaaa ttctcgtaca atgccagacc ttgaaatgct
     4321 gtcactttta catcagactt taggtctgcc gattgatttt ccttctattg aatcagctac
     4381 cgaatcaata gaaccaacat tgccggatga acctcttgtt atcaagaaat cttattcatc
     4441 aaaacaaccc ttctcactca aaaagacact atctttcttt ctgctcgtct tttgtttggt
     4501 ctatcttttt cttatcaatt atcaatataa aaatgaaacg gttgatcgat tcaatccttt
     4561 tttgaaacca caaatcggct ataccacact ccctgtagga tacgtggaaa attccatcga
     4621 acattgggta tgtaatgata tctttgttca tggtacatgg ctgtcttttc acgctggaga
     4681 ggtagaaaaa ggtaagaatc atgctgtaat caaacacaaa ggaaaacacg tcatgaacgt
     4741 tcgcctgctc acttgggaag ccattccctc agattaccaa gaaatcctca tccaagacta
     4801 tgacgatgcc ttaaaaagaa gccctcagga gccagatagg cattaagtag gagatgaacg
     4861 tagagataga aaaaatcaat gcacatgatc ttatcaaaaa aaaacagggg tacaaattaa
     4921 ttgaaaaagc tttgagatga aagttcctaa ataaaaaagt tcagaatgat aggtaaaaaa
     4981 atgactaact attctagtat taggctattt gtattattct tctgttattt ttatatcaaa
     5041 aaatccagaa ataactaaat ataggtattt ctggcttata gtataatatt tacgccacag
     5101 cccccaatga ttctttttgg aatcatgatc aaggacggac atacacaaaa atattttgcc
     5161 cattcgatcc gaatagatta acgactaatc tagtaagtaa tatagattgg tacgtagtca
     5221 aaccagcaaa tggaaaagtt gtctattcca ccccaagggc tttaaagaca gcatcttccg
     5281 gcgtttgata aaaaatcaaa ttaaaggtac ccattaattc tgggggtaca gttccaatat
     5341 cagcagcgct tatcataggt acaagtactt ttttcgctcc ggcatctttt gctacttgta
     5401 aacagttagc taaattatct accttaatca tagaaccacc aattgtaata tcccctaaaa
     5461 taactaaact tggtaataca ggtttattta aggaaactga gcataaagct attatagttg
     5521 ataacgataa tgtttttgtc ataccaatgc cattcatatc ttgaacatga acgacaaaat
     5581 cttttgtcgt caaactaata gactgactaa cacttccata attagctctt aaatatcggt
     5641 atgccgaatc catcgcttct cttgctccag catctgaatt gatcccagta cgttcaaact
     5701 ttccatttcc cgaagtcatt tgcgtttcca atttataggt acctaattgc ccagttttgc
     5761 cataagatac cgtgtacaaa tgccctggct tccctaaccc ttctggaatc aaagttcctc
     5821 caccttgttc aggaacagaa acatactctt ctcgctcttc ttctaaatca atgtatgaaa
     5881 aattgacatc ataaaattcc attccaccaa ttttctttaa ttgttctttt acacgacgac
     5941 gcatttcaag agcaaatctt agtatttctt cgacatcact tttttcaaat ctaccatcag
     6001 gatacaacag tttcatcatc ccagatacaa tccgacgaac tgcaatcgta tcccgctgat
     6061 ttaattgaga accaagtttg aaatatttat caaaactatc gccaaaacta acttttcgaa
     6121 gctcacgcat gacttcagaa aaataatctg taatgaagcc atagtcattt gtaaaaaaat
     6181 ctggtcgata tttaggaatt tcccaacctg gtaaataaca atgcatccga tccaaaaagg
     6241 ctgaatccgt ccccataacc tccggaaatg gctcaaataa attcgaagtt ttcaatatta
     6301 catcaacgct ttgattaata tttcctacaa aagccattga tgctgaagca ttcttttctt
     6361 ctttgcctct agcaaaagat cctgaagcca tgtaatcttt catgatttga acgccatcat
     6421 tatccttaaa tttaatccca gcaacttcgt caaaagcaac acagtcccac atacctacaa
     6481 gtcccattgt acgactagcc atattataga aaaggtttgc tactgttgtt tgtccacctg
     6541 aaacaaggat tgagtttggt gaaatttctt tatatacatg cgatttacct gtaccacgtg
     6601 gtcctagttc acagagatta aaattatttt caattaatgg aataagtctt gctaaatgaa
     6661 gccattttac ccttttatta aattgtgtag gttccatacc aatggaccgt agaagtaagt
     6721 caatccattc atttgtagta aactccttgc gagccatctt gtaagcctca atgtctaaac
     6781 caggcatttg gattggatca accttttttg caataaaagg gctattccaa cgatcattct
     6841 catcatacca atattccatc gaaacaatac accagatccc cccacctaat aatcgatcaa
     6901 attttttcgg atactcttca ctaatttgaa cattttttaa tcccaggtta gagaattctg
     6961 cttcataaca atctttcttt tcatttaaat gtacagttac tttatcaata actgtataac
     7021 cacccaattc acgaattttt gcttttactt tttctgcttc atcaggacgc acatagtttc
     7081 gagcaagaat atttttaaca ttttccacac ctttttcaac caatgtttca tccaatgacg
     7141 aagcatacat accaagtaaa tattccaata cataaacagg tacattcgca ccttctttaa
     7201 ttttctttgt taaatctttt ctgacaatct ttccgggaaa gaattcagat aactttaaaa
     7261 tttcctgttg ttccactaga attcctccat gtgttcagtc attataatag caattaaagt
     7321 atatcatact taaccagtag gcttgataat acaaaagaaa aagtatccac aaaaaattgt
     7381 ggatacctcg tttttatata tcaaaatcaa atgattctgc aaaaggatta tctataatga
     7441 attttattcg ctcggtcaaa tcattttttt gattttttat cactaaataa taagtatctt
     7501 caactgtata tcgacgattt acaaattcaa aaacaaattt agtaaaccgc tgatttgcat
     7561 cttctgtttt tgaatccgca acatagattt cttcatttga gatacgttca ccatattcat
     7621 cttcaaaata aagccagaaa gatgcggggt cataaacatc actaattgca tttttttgat
     7681 agaattctaa cgtggttgat aatcctgtaa ttctgcggtt gctagtcatt aattcaattg
     7741 ctactggttc ctttgcattg ctacctttgg ctaccgtaat ttctagaaca gggactagca
     7801 tttcttgaat agatgaccct ccatgaacgt agctttgtcc cccgccagct ttcttaaaaa
     7861 tacttgaagt aatcgggaca caaatgtttc tagtgtcatc atttcctaaa gaatctccca
     7921 aagagtaatt tttcgtacca attatttcgt attcttcttc actaagaata aaacgacgtt
     7981 ctacacgatc tttttcattt ctagatggat tttcaatctt atctacttct tcaacgtggc
     8041 ttcttttata aataaatcca tgatcagccg taactataaa acgatagata ctgccatcat
     8101 tatgcaagcg attaataagt cgggaaattt cgcttatcgc taaatcacag gcctcaaaaa
     8161 ctcgattttc attcgagtga tcacctgttc tatccacttg atcgtgataa atataaatga
     8221 ctgatttgcc attaaatact tctcgtacct cagccttatt catcttaacc acatcatcat
     8281 aatttaaagc tacagcattt gcatctcgtt gctggagaat cgcatttcga ttggataaac
     8341 cattcgtttt tttcccatca atcagtactt ctgttccttc attgtaagtt agttgattgt
     8401 ttggtaataa ggcggcttta ccgaattcag taactgaagg taatacagaa agaaagctag
     8461 ccatgtttgt tgaatacttc ttttctctaa ctaacttacg ttctaaattc atccctaact
     8521 catatcgaaa agcatctgaa ataataacta cagttttaac agagcgtttc attacagctt
     8581 gattataaaa ttttcgaatg gatggacgat tcttaaattc aaaagtttga ttccaaattt
     8641 gtcctgattg atcaagaaac ttcgaataat ttgcttcaac taatcgatac aattggccat
     8701 atttttgctt ttcttctata tgatcactat tccaaataaa tttccgataa tgcgtatcaa
     8761 cccaatacat ttccttctca tattgagtta gcaaattatc catgttcatc ggcaagcaca
     8821 atctgttttt atccacaaga taataagcat gtttcaataa ttgatactca taaatataat
     8881 ccgatgcgaa atgcattttt tcgcgctgct ttaaactatc aaaaattgtt tgtcctgcaa
     8941 ttttcgttgt agtgtcagaa gcctttaatc gttctgcata aaacattaat atttttgcat
     9001 gaatctcaga aaatacgtct gcttcaatta gatcatcaat ttctatactg tctaataatc
     9061 catcaccatc aatagcctga tatacttttt tagatagctg ttcaaagctt tccatgtaac
     9121 gtgtatcatt cataaactga tccatgaaag taataatgtt tgctttcaaa tgtagtattt
     9181 caaatccttt gaacttcttc ggcatctctt gatcaatttg ataataaaaa tagttcaaat
     9241 aaagacacat cgtcagcttt tccaatgttg gctggctatc cgtaaagcca aatttcattt
     9301 gaataaattc ccaaaattta gctgttaact gatacttatc aagttccctt aaaggattct
     9361 ccctactttc tgtaaagctt tttagaacta cacgcagtaa atcatttaat cgaatacttt
     9421 tcgatttcaa caaaacagcc attatcatta attcaatcgt ttctgattta ttaattgtca
     9481 tatgcgtact aaattcacca aaacttctca tgcgatcctt tgcattaaag aaattacgat
     9541 actgtttaac tgtctgatga aatctaagtg gaatagccaa ttcattcatg ataatcgcta
     9601 agcggtctgc actaaattcc tctgaataat tcaatacact caacaaatga ttatcttcat
     9661 ctttatcatc ccaacgagga aatggcgcat agattaaata gttagtttcg ggatcttcat
     9721 attccaataa acgcttagtt ttaaattgtg cgtccttttc taatcgatag atttttgcat
     9781 tttgaatgac caatgagtca atttcttcaa gaaaatcctg atttgaatca aaccaaaaaa
     9841 tgattttcct acatccataa ttaggaaaac gttgatttaa tttttcttca atttgttgcg
     9901 tatttatatc tgtcatcaat ctcacctaat cgttctcttt ctatacccct tatattgaca
     9961 taatttggat aatttcgcac gcaatttaag aataaaaaag aaagttgtta gaaaacaact
    10021 ttcttagata actatttttt tggttctttg accccttttg caaaaatttg gaatttctcg
    10081 ccggttttag gatcacgctg caacttatca taattgacaa ttacaccatc atctaagtct
    10141 aattcaattc gttttaatgc taagtgaccc aacttctcgt catactcacg aacttctttt
    10201 aattgcttct gaatctttac aatttctttt gtcattgcat ttctttcttt tgggttatca
    10261 cttgttgaaa tttgttgctg acgcaattcg attcgattgt catacgcttt ttgtagttcg
    10321 tgtaggtatt cagtacgtac ttttcctaat tgatctggtg tatagcgatg caagtacatt
    10381 aacgctttaa agccattttg ttttcctgag tccaattgcc aataaattgg tcgattggag
    10441 tatgttttta aatggtcttt gtaaaaatct tgtacgaagt aacgacgaat aatttctcga
    10501 cttgtccctt tgccacctaa agtgtcagcg ataaactgga gattttcttc aagggtttct
    10561 ttaccaaaag taatctcaac gaaatcaatg aaacgattga caatgtcaat ttggctatcc
    10621 ttgaaatact catcatcggt gatgagtaaa acattatcag tatcaggttt aaatttttga
    10681 tagcgggaag gatcccattc gccgccagcg aagatgagac catcttcgtc tagggagtaa
    10741 cgaccgaaga tacaacctat tgaataagaa ataaaatcct taatttccct cgccttatca
    10801 ggtaaccttg cagagatata ttcatcttga acctcttttg ttaattcttt ttcaagagaa
    10861 taatgattaa tgaatatgtc gttcagttcc tcttcattcg attttagagt attaaattga
    10921 ttaaccaact gcctacttag acagttaaat gcctctgaga gatctttttt aaacatataa
    10981 cttaacaaaa tgtttcttct aaactcgtat gacgtttcaa gagcattata gtcttttcta
    11041 gctatatcga tattttcatc cactttttct cttatttttt ttaattttct ctcagatgta
    11101 actttaagaa aaggtagttt tcccattggt ccttcatggt aatccaaggt gggagaaata
    11161 aaagttaaaa agtgattaac taatttagag tttacatagc caaatatttc ttcaaagtca
    11221 gttatatcct ttgagaacag aacagatcct ttagtttcaa aaattgcctg attctgcaat
    11281 ctaaaagata cttgtccaga tgaaagtgtc gaccaagtaa ttcccttttt aaaatagtaa
    11341 ttaagatttt gtggtctaga ttttaacttc ccattttcat taacataatt ttttatttca
    11401 tctccatcat tctcccagtt aaccacgtat tcattgtttc cataccattt tctaaaacga
    11461 ccacctttag ttgtaggaaa ccattttttt ctagactttt tagcgtcctc taatgtttta
    11521 gcatcaaact ttattttgtt aaattctact tcaaaccaat accgcaaaaa tttattgtta
    11581 tttccggttg ccaacccttg ttttgcatct gcaacatcgt tagttaattt tccttgagca
    11641 aaatcagtga ttacgttatc gcttgcccag taagcaatcg gcattcctgg gatttttttg
    11701 aagttgtctt gattagtact ataaaaataa tcgcattcac catgctgatg aattgcttct
    11761 aacgttttaa ttttttgaac ttccattcca cctttaaaat ctgataattt tatatagctt
    11821 ccatcttgtt catttgaatt ttttatcgta aacgtacaaa ttggaacggt tgcttcttca
    11881 aaagctgaat actccatttg aatcaaacta gaaatgtttc tttcattaat taaaaatgag
    11941 cgtaactttt catagctaga aatgaacatc caaacaaatg gcgtcataaa ggaagcatat
    12001 ccattatcaa tcagcatttg gctattcaat ttaataaaca cagaaaatag atcttttttg
    12061 acatcagaat agttatcatt tacatatttt tttaatttct tatccatttt attaagataa
    12121 ggcggattag tgactaccgc tacatattta tcaactagca tttcagccac gtcaagaatg
    12181 tgcaagtatt tttcttgtat ttccttcatc ccataaagaa tatccatatt cgtaaattcg
    12241 ttgaattttg atatatactc tcttactttt cctaagtatt ctttagttat tgcctgatta
    12301 tttttatcga ctaacttaat gattgatcca agttctttcc cattttcaaa ttgatcaaaa
    12361 attaactctt tgtattcatt ccagcgttca ctatacttat cattgttcaa ttcactaata
    12421 cgctcttgat actcgtctgt cactttagga gaatcaataa actctcttaa attcggcgat
    12481 agactttttt tcaaaaaaag acgatcgtat tgacgccctt tcatcataat tgcaaaataa
    12541 gacaattgat acgctctttt atcgatttca agaccatata aattgtattc aacgattgca
    12601 cgtgcagcat cacgctcaga atatcctaac gtaagataaa tttccatcaa tacatcgaag
    12661 gcgtaaacta aaatatgccc agaccccatt gcgttatcga taatttttag ttcttctggt
    12721 tttaaattac ttacagatgt tgcttgttca aaagcatatt tatctggaat taagtatttc
    12781 aacttatttc ttaattcact attgggataa tgatctaacc aatactttcc taaactattg
    12841 tcaaccatat aacgaacgac ccaatctgtg gtaaatagtt gagttgctgc cggaatatct
    12901 tgttttttga caatcccttt ataaatattc accacttgat tatgtggctc actattatag
    12961 tactgataca accacccaat aatctctacc tgtccaccag cttgaacata gaaatcatcc
    13021 tctggaatac ttaaaagatc agcgattaca ccatcttttt taatataaga agctgtaaac
    13081 aaaagttctg tataatcttc tacttcttca aatagctcag gtaagtaatc attcaattga
    13141 ttacagattt ttataaaaat caattgatac agcttgtcca ttgcttctgc tgaattatct
    13201 gttttccaag ttctgacttg ttgctgttca ttactagata attcttcaaa gatatcggca
    13261 tcataaatat ctgtaataat atctggctca attttcccac ttatttcact agataataca
    13321 cgatacgtgt caggtaaata atcatttacc tccataaacc gaatggcaat caatcggtta
    13381 aaccacgtat aagcaacctc ttcgattaac tcttgaaaag cttctgaata agaagtgaca
    13441 gaagcacgtt gttttagttc tgcaatcaat ttgtttcttt gcgctatttc ttttccttga
    13501 attcttattc ctgatttttc gatttcttgt aaattgctat caattttctt cacatcttca
    13561 atttttgatt catcaattcc taaacgtttt gctttagtca ttacttcatc aattaatttt
    13621 ctacgggcat tgaccgcgaa attctttatc gctttcttat ccatttttct ttcccttcta
    13681 tatgtaacct ttgaaaagac aggcaccttt ttatgcctgt cttttcacta gaattgaatc
    13741 gtgatttctg tattttcttg aagttgttcc ttcagttttt gattaatttc agctgttaac
    13801 tcatcaatat ctgctgctga cttaatagtt ttaacatgaa cgttaagtgt tctcattggt
    13861 actgttttct gttgaatagt aggttttact atcgctattt cttcagcttt tgctgcagag
    13921 tctatttcat ctttttcagt ttcacgaatc aagtttgctg cttcaatttg tttacgtgct
    13981 tgttcttctt gacgtctgcg gttttcttca ttatcgaaac gttgattaaa gatcaaatac
    14041 tgttctgcta cgtttaatga taagaaaaca agatcatcta tagtattcgc ttgttcaatt
    14101 tttgctttca gttcttgaaa ttcttggtca actattggta caaattgatc cttaaaatct
    14161 tttccttcta ataatttaaa tgttgcttgt ttttcttccg ttaatttatt taatatttcc
    14221 tgactttcca actctaacag ttgattatat tgttcaataa attcttgaat caactcattt
    14281 agtttcatca tatctgaata aggatcagcc tttttcaaaa tggttttcat ctcatgaatc
    14341 aattgatcaa tttcttggct agaagtttga cgccgacttt tttcgattaa tctaatttta
    14401 tcttgtgcat tctcccagat ttttttctga gtactatttt gcttgtggaa atttttaatt
    14461 ttatcatatg tttcagccca gtcgaataat tcatcgcgat attttttgat tgtataaaaa
    14521 aagtcttcaa catctgacgt ttgaacaatt acttcaagat atttttttat ttgctcatat
    14581 tcataagcac tagggtaggt ataagtgttt tgtaaatcaa aataagagcg taaattgtct
    14641 aattgacctt ttaaatattt cttgaaattt tccattgtat gttcattgtt attttcatca
    14701 acaatattac gactttgaaa cagttctcta ccgatttcgt caaccaattt ttttgctttt
    14761 ggcgaaatca ccgcgcttgg ttcaattttt atcttttcac tttctcgtcg tttttgaata
    14821 atcattacaa tactatttgg atcatccatc cgtgaaattt gtacattgtt ataaagtaaa
    14881 tcaattttac cagacatgaa aagtttagcg atgcaccatt ccacatcaag ttctgtgaat
    14941 ccatacggtt gttttgtttc aaaacgatca cgaattgttt tcatcgtaat cgttaggtga
    15001 tttctctttt gatcattgat aaaattgaaa atttcctgaa ctgcttgctc atttgatgga
    15061 tcttcaataa tttcattttt gctttctttc actaaatata agatatcttc tttttcttta
    15121 ggcgcatcaa tttggtttaa tttatggtat acttcttcag ccattttctg aagtgctgat
    15181 tggaattttg ctttaaagtc ggagcctgtc agttgaattt ctgcaccgtt atagaaaaat
    15241 tttgcagatt tcaacagttc atttaattga tcgttgatac ttgcacttag ttcctgtaat
    15301 tcaccagatt tttccgaacg aatcgcgtca aagttcttta ctacactcga ataagtcgtt
    15361 gttaaataaa gattcattct agcatacatt gctatatcat ctttaaattc tttatctgat
    15421 ggcaattcta cgaataataa gttacgggaa ctcttaagcg caagttctgc ctcttgacct
    15481 gctaaatcag agtatggtga aataaaatta actgtcattt cagcatttcc agcttgcttg
    15541 tatggacgtt gatctaacac ttgtgcataa gtaaacgtat accggttttt aaatttattt
    15601 ccacctgaca tgcctaattt ttcataggca aaactatttc ggtctagaac gccgtcaaaa
    15661 attcgtttag atagttctac gatcaaatca ctatctgtaa tattttgatt tttaatctca
    15721 cgagtaattt cctgttcttc atcagtttgg aaaatataac tatctaagtt cttccgcaca
    15781 aagttttcct taattaaaag gtccaatgct tcttctactc gttttttagt ttccaggcgg
    15841 tcttcgttga tatttcttat catcaagctc gtaatatttt caacatttgg tttgatggct
    15901 tgatcaacat atttaatcat gaataaaatt cgtaagacat ttacgttaaa gttatctata
    15961 tcagttggtc tttgaatatg agaattattt gtagcttgtg taatcactcg gctaattgct
    16021 gtatcaatcc aacgttctaa cgagtaataa aactgatcta aagaaaccaa actggttgta
    16081 tcattttcct tatttcgttc agcggcttct ttaaacattg ctagtaatga acgttcacca
    16141 gacgataagt ttgatccttg atagctgtga ttacgaatct cggttagaat ttgccccaat
    16201 aaattgaatt gatagggaat aaatggataa acttctgcgt aattttcagc atcttggaag
    16261 agctttaatt caggactatc aacaaagagt aatttatttt tcaattctgt ttgaacagtt
    16321 tcaaattgta aggctaaaga atcattggca aactcttttt tcttcaaaat acgttcttta
    16381 attacttcat cggcatttgc tgaagataaa ctcacagttg tggcaaaacg atccttgatc
    16441 ttagaaaaat cattatcaga attgttatca aagtttgtta cgtgatcaat cgcttcctga
    16501 cttgtcacta atacccaagc cttaccttca caaattcgac ccaaatcttc cacaacggtt
    16561 tgtaactgca aagtcaactc acgattaccg ccgatatatt gtccgacctc atcgactaga
    16621 aaggcaatat gatggtctgc ttctttggaa tcaagatagc ttttaactag atttgcaaag
    16681 tcatcaatgc ttattaaata atttcctgat gctttttcta aaaaattatc aatagcggct
    16741 tcactcataa aatcgatttt aagtgccgct tctttgatat catcttggat ccaatcaaac
    16801 tcatggcgct cttcttgcca ttttgcccca ttaatctctt caaaagcagc tttgaatgca
    16861 tcaaactttt cttctttaat taaccgacgt tcaaaatctg ctagatgtgg aatcgtgctt
    16921 aatccttgca tttcgttaaa tacctttaaa aaaacattca cgattccgtt tttgtctttt
    16981 ttaccacctg catcactttt tgaatcaata ttaaacagaa taacatctgt atttatttgt
    17041 gagcaccgtt ccatcgttgc caaagtcagt ggatctttga ttttcccatc ttctttgaag
    17101 taatcaatgg ctttcttgtc atttatttct cgatttgcga gcaagtaaga aataattttt
    17161 aagaaatgag atttacctga accaaagaac ccctgaatcc aaacacccat tttatctgtt
    17221 ggtgtattga tactccgttg gtaggcatca aaaattgttt taaagtgttt ttgtagttct
    17281 ttcgtaacca cgtattcttc taattcttgt tgaacatttt catcctttgc ttgaccgacc
    17341 ttaataactc cttgaatatt tcggtcaatt ggttttgcaa aaatatcttt aattagcatt
    17401 taatctccta ccttccatcc caactaaatt ctaaatgctc gataataatt atcatcttta
    17461 ttggtattaa gaattgtcaa acttaattca tcatattccc ctggatacat catcacaact
    17521 ggtacgcgat caaatacctg atgcatttta tttaaaatag agtgcgctct aattaatgga
    17581 tataattcgc caattcctgt caggaacaca actgattttt ctggtgtatg ggcatccaca
    17641 tattcaaaaa atacattttc ttcctcaggt aaatccattt tcaataaacg accaatgctt
    17701 tccactaatt tttgtaaacc acgtcgttct tccatcttaa tcgctttttc agtgaaaccg
    17761 cgtttttcta gatactctaa cataatcaca tagagattat aaactttcac ttcataatta
    17821 tcaccaacag atgttccaga tatatcatta atataatctc ttacaattaa ctcgtctttc
    17881 ggatcgtagt caaagatata atagcctact tcattaccta aaccacgatt caataaaaaa
    17941 tcttcttttt gaattacttc attcaacttt tccaaatgct cttgaatcga taatgacatt
    18001 ataaaattcc tcccagtgct agtacaatat ctttacgatt cttgtcaact aaccattgaa
    18061 tcaatcgttt atctaataat atcggagtca ttcgtccata ttctttttcc cctctgacta
    18121 atcctgtttc aattagattc gtttgataag cggcaataag ccgtttaatc gtataatctt
    18181 tccattttga aatatcttct gaacttctcc gcttctctgt aaaaaaagaa cggtaatcaa
    18241 ctggtgttaa ttgcaaattt cctttacgta gattttcagc atatacctca gtcataaatt
    18301 cattaaaaag atcatcaatc ataaggatgg aaatcaaatt cactaacttt tgattatcta
    18361 tatttaactg attgaaaagt tgcaatccag cttcatccag ttgactaact ctccttttca
    18421 cgttctgata gatttctttt tgacggttac tgctcactgc aaaaaaaata ttttcatgtt
    18481 catttttctc tttaatttct atatctgtat atccttctag tcgtaattga atatataact
    18541 tgaactctga aaaccaaaag cgtttagaaa ctagaccagc agaatatttt ttattcaata
    18601 gattgcctcc taatcatcta taattcaata agtaaacaag tgttttttgc tcatcgttag
    18661 cattcatttt ttagtatcta ctatacaata catatttatt ata
//
LOCUS       DAZKNH010000051        18504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000051 DAZKNH010000000
VERSION     DAZKNH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.051"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACG8MG_001645"
     CDS             99..746
                     /locus_tag="ACG8MG_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0375688.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACG8MG_001646"
     CDS             1139..3784
                     /locus_tag="ACG8MG_001646"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFQ0375689.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACG8MG_001647"
     CDS             4094..5407
                     /locus_tag="ACG8MG_001647"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFQ0375690.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACG8MG_001648"
     CDS             5397..6050
                     /locus_tag="ACG8MG_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0375691.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACG8MG_001649"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACG8MG_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFQ0375692.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACG8MG_001650"
     CDS             6947..7147
                     /locus_tag="ACG8MG_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0375693.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACG8MG_001651"
     CDS             complement(7401..9080)
                     /locus_tag="ACG8MG_001651"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0375694.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACG8MG_001652"
     CDS             complement(9082..9294)
                     /locus_tag="ACG8MG_001652"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFQ0375695.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACG8MG_001653"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACG8MG_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFQ0375696.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACG8MG_001654"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACG8MG_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFQ0375697.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ACG8MG_001655"
     CDS             13269..13466
                     /locus_tag="ACG8MG_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375698.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="ACG8MG_001656"
     CDS             13619..14104
                     /locus_tag="ACG8MG_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFQ0375699.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG8MG_001657"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG8MG_001657"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFQ0375700.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="ACG8MG_001658"
     CDS             14981..15508
                     /locus_tag="ACG8MG_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375701.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="ACG8MG_001659"
     CDS             15599..16336
                     /locus_tag="ACG8MG_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0375702.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="ACG8MG_001660"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="ACG8MG_001660"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFQ0375703.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="ACG8MG_001661"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="ACG8MG_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFQ0375704.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3199 c         4022 g         5299 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgt
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DAZKNH010000052        17369 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000052 DAZKNH010000000
VERSION     DAZKNH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.052"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(122..667)
                     /locus_tag="ACG8MG_001662"
     CDS             complement(122..667)
                     /locus_tag="ACG8MG_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0375705.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(679..1371)
                     /locus_tag="ACG8MG_001663"
     CDS             complement(679..1371)
                     /locus_tag="ACG8MG_001663"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFQ0375706.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1390..1674)
                     /gene="macP"
                     /locus_tag="ACG8MG_001664"
     CDS             complement(1390..1674)
                     /gene="macP"
                     /locus_tag="ACG8MG_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFQ0375707.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1687..2250)
                     /locus_tag="ACG8MG_001665"
     CDS             complement(1687..2250)
                     /locus_tag="ACG8MG_001665"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0375708.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2478..3140
                     /locus_tag="ACG8MG_001666"
     CDS             2478..3140
                     /locus_tag="ACG8MG_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFQ0375709.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3264..4460
                     /locus_tag="ACG8MG_001667"
     CDS             3264..4460
                     /locus_tag="ACG8MG_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFQ0375710.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4561..5937)
                     /locus_tag="ACG8MG_001668"
     CDS             complement(4561..5937)
                     /locus_tag="ACG8MG_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0375711.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5974..6840)
                     /locus_tag="ACG8MG_001669"
     CDS             complement(5974..6840)
                     /locus_tag="ACG8MG_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0375712.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6929..7954)
                     /locus_tag="ACG8MG_001670"
     CDS             complement(6929..7954)
                     /locus_tag="ACG8MG_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0375713.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7972..8646)
                     /locus_tag="ACG8MG_001671"
     CDS             complement(7972..8646)
                     /locus_tag="ACG8MG_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0375714.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8830..9723)
                     /locus_tag="ACG8MG_001672"
     CDS             complement(8830..9723)
                     /locus_tag="ACG8MG_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0375715.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9797..11494)
                     /locus_tag="ACG8MG_001673"
     CDS             complement(9797..11494)
                     /locus_tag="ACG8MG_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0375716.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11487..12800)
                     /locus_tag="ACG8MG_001674"
     CDS             complement(11487..12800)
                     /locus_tag="ACG8MG_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0375717.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12800..16741)
                     /locus_tag="ACG8MG_001675"
     CDS             complement(12800..16741)
                     /locus_tag="ACG8MG_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0375718.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16850..17308)
                     /locus_tag="ACG8MG_001676"
     CDS             complement(16850..17308)
                     /locus_tag="ACG8MG_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0375719.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4872 a         3900 c         3023 g         5574 t
ORIGIN      
        1 acggaaaaag aataagtttt tttaaaataa cgtataaaaa agagcataaa acaaagtcgt
       61 ttgttttatg ctcttctttt attattttgt gctttttatt ttatcaaatc actttgtttt
      121 tttataaaat ttctgcgcct agtgtggact caaaatgtct caatgcccat tcatgtccaa
      181 cgggatcgaa acttgccacg gaatctttga aaatatggag tttgtatcct agattatacg
      241 catcgactgc tgtatgcaac acacagatat ctgtacatac gccggtcaaa taaatatctg
      301 tgatctggcg ttctcgcaaa cggatgtcta gatctgttcc actaaaagcg gaataatgcc
      361 gtttgtctat ccaataaaca ttttcctctt gctcatagtc ttggtataaa ctttttaaag
      421 agccgtacaa ttcccgtcca cccgttccta tcacattatg cggaggaaat aagcggttct
      481 ccggatgaaa ggaatctttt ggatcatgcg catcaatggc aaaaacgaca aaatcctttt
      541 gatcaataaa cttctttgta tgagcagtca gctttttttc aattacttgt cctgctgtcc
      601 cagtagtcag ctttccatcc tctgcaacaa aatcatatgt gtagtcaatc gagatcaatg
      661 cgttcatctt agtcctcctt atacgagatg tttgacaaat tcgatcacca tttctgctga
      721 tcgtttgcct gcgtcttcga taaattcgtc aaagctttgt gtcgccgagt gatctgctgt
      781 gtcgctcatt gcacgtacga tcaaaaatgg gatattgaac tgacgagctg tttgtccaac
      841 agcggctcct tccatttcgc aagctaatgc ttcagggaaa ttcgttaaga tttctttgat
      901 tttatctggc gagtcgacaa atgtatcacc tgtaacgatc aatccttctt ttgcgttcaa
      961 attcgttgct tttgctgctt taaccatttc actgcgcaaa tatgtgcttg cttcataata
     1021 tagaggcatt cctggtaatt gtcctgggtt gtagccaaat cctgttgcat ctgcatcaaa
     1081 ataagctact ttgtctgata taacgatgtc acctacttgt aagccttcac cgattccgcc
     1141 agctgaacct gtattgatca ccatgttcac gccgtattgt tggatcaaca gacttgtggt
     1201 aatagatgct aagacttttc ctataccgga acgtacgacg atcacttcgt ggttacctaa
     1261 tgaacctgaa ataaataaag cgcctgcgcg ttcccatgat aatggttcgc ttaggttctc
     1321 tctcaaaatt tttacttctt cttccattgc gccgataata ccgattttca tctttttaat
     1381 tccttctttc tatataaatg cgattgctaa aaaaaccaca cataacatta ggataacaat
     1441 aataagagat ttcatcaaaa aagagttcca tttggtcgtc ttttcccgtt cgctgatcct
     1501 tgttttcgtg attggtttat tctttttgct ttctttacga taaaagctgg caatcttttt
     1561 ttcttcttgc tgataagctg tttctgcttt tcgctgtttt tttaatgact cgcttgcttg
     1621 tgcttgttgt cgcttgcgaa gttcgcttcg cgtaataagc ggtcctttgg ccataagccg
     1681 atccctctac tttcctttgg tcaatagttc ccagtattga atcgcataaa tcgtctttgc
     1741 atcacaaatc aactgatctt tcatcgcttg ttgcgcctct tcaagagtta gatgatacaa
     1801 ctccagtact tcgtcttcat cttgtgctaa aggattctcg accttctcta ccccttgtgc
     1861 atgataaata tgaaggactt catttgcaaa acctggtgaa agatacatcg aagtaagatg
     1921 gctcagcgac ttcgcacgat agccagtttc ttcttctaat tctctagctg ctgtcatttc
     1981 gggattttgt ccttctcctg gatcgatttt tccagctgga atctccaaaa tcactttttc
     2041 taaaggtttt cgaaattgtt tgacaagaag cagacgatct tgttcgtcaa aagcaatgat
     2101 cccgacaccg ccaggatgaa ataccagttc tctcttcgaa gttcctccat ctggcaaacg
     2161 aacttcgtct acagcaacat caataatttt tccgctatag atttctttgc gactgattgt
     2221 tttttcttca aattgctcat tgttcagcat atcttctcct gctttctttg taatcataac
     2281 tgcttttttt tcatttttca atttttctca aaaactcctt attagtttac catagataaa
     2341 ccttagattt tctctaaata tagaaaatcc ttctttagtc cgatactctt tctagtagtc
     2401 cgtatgctaa aataagtatg taatcgaata acgaaaagaa ttcaatactc aacgataaat
     2461 gacatggagg gtttattatg ataaaacgaa attggccgtg gcttctagca ttggtcttgc
     2521 tatttctctt tgtccgaaac aatagttttt cttttgttct aggactttta ttgataggtg
     2581 tagttttagt tttgtgggga ctctttggtt cgcgcaggaa aaagggaaaa caagaagaaa
     2641 ttcctgcact gactgacgaa cttgaaactc actatgaaaa aagcggtatg acagcgaatg
     2701 aaatttcatt ttttcgacaa acaatgaatc aaacaaaaaa tgagatcaat caattgcaaa
     2761 tgaatatgca gcagacagcc aaacttaaag caatcgatct ccgccatgat ccagtcaaag
     2821 cagcgaaagc tttattcaaa gaattggtga aagaaccaaa ccgactgcat gaagcaagtc
     2881 agtttcttta tacgcatctg ccgaatctag ttgatttgac aaataagtat aatgagatca
     2941 atgaccatga gatcaaaaac aaacagacct atgaaaaact agaagaaagc gcgcagatca
     3001 tcgaccagct gtcagcttta atcgcacaag attatcaaaa attcgttgct gaggatctag
     3061 atgatatcga cgtagagata tctgttgcga aacagagctt aaagcgtgat aataaatatg
     3121 aaccagaaca aaaggattaa ctcttttgtt cataaaagaa gactcggaac attcagatga
     3181 tgtctggctg tttcgagtac tttttctaaa gtagagacat tcatgtaaga atcgagaaca
     3241 gattcaacaa ggaggaattt tttatggatc ataaaccgac tgaaacaaaa gatgttacac
     3301 cagttaacga tacacttgaa gatttattga acaacccgtt ttcaactcca acagatacgc
     3361 tgaccacttc acaacaagcg gagattgatc agctccaaga gcaacagaca gctgctcgtt
     3421 taattgacaa actaccggcg gaacgccaag aacaagctaa acagctagca gcaaaaatcg
     3481 atgctaatga tgcacagtct gtcatcagct acggatcggc tgcccaagca aaattaagtg
     3541 aattctcaca atcgatgttg aatcatgtgc aagcacaaga tattggtcct gtaggagatt
     3601 ccttaactga actgatgtat cgtctccaag aagccaatcc tgacgaactg cgtgccggag
     3661 aagggaacat cttccagcgt gtcttcggga aggtcaaaca atcgatctat gaagtgacag
     3721 caaaatatca aaagattggt gcacaaattg acaagatttc agtgaaatta gataaagaaa
     3781 aagacggtct gctaaaagat aatctgatgt tagaacagct ttaccagaaa aacaaagact
     3841 actttgatgc actgaatatt tatatcgctg ctggagaatt gaaaatggaa gaacttcaaa
     3901 caacgatcat tccagaagcg atgaaaagag cagaagaaac tggcgatcag atggatgttc
     3961 agattgccaa tgattacacg caattcctag atcgtctgga taaacgtacg catgatcttc
     4021 gcttagctag acagatcacg atccagcaag caccacaaat ccgtttgatc caaaacacaa
     4081 atcaggcctt ggctgaaaag atccaagcat ctatcgctac agccattccg ctgtggaaaa
     4141 accaagtcgt gatcgcattg accttgcttc gtcaaaaaga tgcagtaact gctcaacgcc
     4201 aagtctctga aacaacaaac gacttattga agaaaaattc agaaatgctg aaaatctcag
     4261 ccatcgaaac agcgaaagaa aatgaacgtg gaatcgtgga tatcgagacc cttcaaacca
     4321 ctcaaaatga tttgatcgaa acaatccaag agacattgcg tatccaaaaa gaaggaaaag
     4381 aaaaacgtcg tcatgcagaa gtagagcttg gacacatgga agaagatctg aaacaaaaac
     4441 tgttggattt aacccaatag atcacctttc gcttgttact aaaacaaacg cccaacaaca
     4501 gtttattaca actgttgatg ggcgtttttc ttatctttct tctaacttct ccattggagt
     4561 ctatttctca ttcatcttgc tgtgtccata gccatagcca aaataaatga tcagtccaat
     4621 aaccattacg ataccgaaca cgatccatgt cacagtttgc agacgaagca tcaatacaat
     4681 acaagtcaag atggagacga ttggcaatag cggaacgaat ggcactttga attctccagc
     4741 ttttggtttg ccaaacattt ttctcaactt cagtaagcct agactaacaa gcatcaaata
     4801 ggcgagcgcc atgatattcg ttagttccgc taatacattc aatgggaaaa atccggcaaa
     4861 gaatacagct gccacaccag cggcataagt cgcttttttc ggtgtacgat gcttttgatc
     4921 gatttctgtc ataaacttag gaagtaagcc atccttgctg acagcaaaca gtaaacgagc
     4981 aagtgaatac atcatcgaga tcgttaccgt cagtagcgtt aaaattgccc ctacagcgat
     5041 caccgcgccc actgttccat gtcctacata cctcattgca aaagccactg ggtcattgac
     5101 tcctagttca gtatacggaa cgattcctgt caagatcagc gtcacgatga tgtaaaggac
     5161 agtagcaatc agtatcgaac caatgattcc tcttggcaca tctttttgcg ggtttttgac
     5221 ttcctcggct gccattgcaa cggcatcaaa tcccaagaaa gcaaaaaaca caagagctgc
     5281 accatctagc acaccggaaa aaccaaaagg catgaatggc tgccagttat caggtttaac
     5341 gaaaaatatt cctacaatga taaacaacgc gatgatacca aatttgacat ataccatggc
     5401 attattcaaa cgtaatgctt tcttggcttc aagcgacacg atgtaagtga cgaatatcac
     5461 tacgagcacc gcaatcaagt caatgtatgt accattttcc ggattgtacc cgccagttaa
     5521 tgcttgtgga agttcggccc ctaaactttt caaaaaacct tgcatatagc cagaccatcc
     5581 ggaagcaaca gaggaaaccg ctagtaaaaa ttccccgatc aacaaccacc ctgtcagcca
     5641 ggcagcaaat tcaccaaata caacatacag ataggcatat ggcccaccaa tcactggcac
     5701 tcgcgaagca aattctgcga aacttagtcc agacaaaatg acaacgattg cagctaagac
     5761 aaatgaaagt gacagtgctg gaccagcatt ttgattggcc gcaacccctg tcaccacaaa
     5821 tattcccgta ccgatgatcg caccaattcc aagcatgatc aaatcagacg tttttagttc
     5881 ttttttcatc tcactcgtat gattgagatt gatctctttc ttacgaaaca aatccattga
     5941 agttcctccc cattttaggt tggaaaacag cgattaaact gttttcatat accctaaaag
     6001 gataccacct aaaatgacta aaaggcaacc aacaataaca taaatgagct cttttttcgt
     6061 ttttctttcg ccaaggatga aaatcccacc taaagtagag ataatgatcc ccatttgtga
     6121 aagtgagaag ccaacagcta aaccaagtga tttgactgtt agaagcatgc agatgtttcc
     6181 taagccccat aataatccac agatcatgtt tttccacaca aactgatcga ctttaacttt
     6241 acggaaagca aagaagcttg cgcctaagat cattccgata ctttgaggta agataattga
     6301 catcgcatcc aaattcgccc agttgacgat gatggtatat acaccataac caatcgtaga
     6361 gaagatcaat gctctaaaac ccttaccaaa atcaagcatt ttattatctg tatctatttt
     6421 tccttcgtca tcttgtcggg cagtcaaata cgcaccgctt acaagaagag ctaaagcaat
     6481 aataccgtat acataatctt tcgtttgtgt ccattcatgg aagaaaacgg ctcccgcaat
     6541 cgtattcaaa attaattgaa aaccagtaga caatggtagc cctacagaaa cacccatgta
     6601 cttcattcca tggaactgtc cattttgtcc aacactccaa gctaagccgg ataaaaatcc
     6661 gatcaagaaa atccaagggg tgattacagg actaacaacg aaaaagacaa ctaaagaaaa
     6721 cagaaatgca ccgatcgtca ttcctagtgt ttgttgattg gcatcgccac caattttacc
     6781 acttactaaa ccaatactac cccatgccaa catagggact aatgctatta aaatttccat
     6841 aaccaatctc cttttatttc acatgataag aaacttatca gcgaaaacgt taacaacaca
     6901 agcacttttt ttcgtttcta acgattttct aacgtttttc attataaaaa tttaagattg
     6961 ggaaaaataa agtcattgtt gtcccttttc cttcagttga ttcagccgtc aaatggatac
     7021 ctaatttttc ggccagattt tttgctaaat aaagtcctaa tcctgttgaa tgtgttttgc
     7081 taagtcgacc atttctccca gtaaatccct tatcaaagat ccgtttcaag tcttccgctg
     7141 ggataccgat ccctgaatct ttaactgaaa ggctgactcc ttgcgcttcc ttttctatgc
     7201 tcacaatgat gtttccgtga tccggcgtat atttgatggc attactaagc aattggttga
     7261 agataaaagc gatccattta gcatcggtca aaacagtatg atcctcacca acgatctcgt
     7321 agcgaatatt tttttggata aaataatttg cctgactgcg tatcacaggc tgaatgattc
     7381 cttttagtcc atattcttgg atgaggtagt cccgcgagaa actgtcgagt cttgcataat
     7441 acaaaacttg ttctacgtat tcatcgattt ttgataattc attttctagt aatgtatact
     7501 tttgatcgtc aatatcgaac tcgatcgaac gaactagcaa tttagaagcg gaaagtggca
     7561 ctttgatttc atggacccat gagtcaatat aatctttttg gtcttgttga ttactgattg
     7621 ctgattgcat tacttcttgg tgttcacgga tcagctgatt gatatattct tctaccagtt
     7681 tttcttcttc tgtcctccca ccactcaaat aattttgcaa aggggaagtt ttttgtatgt
     7741 cggctaattt tccccaccat cttctcttgc tcaagtaacc acccagcaga taaaaaatca
     7801 gaaagacgcc ctcaatcaaa gctaaataag caattgtgct caaatcaact ttgatgtttg
     7861 gcgccagcca catcacaaag atcgtcaatg caataaaaaa cagccatccc aagatcaatg
     7921 tccactgatc tttcaaatat ttaaaaaaat tcatcttaac cgctcatcct ttcagggaac
     7981 gatatatccc tgtcctactt ttgtttcgat atatccttgg atacctgcct gttcgatttt
     8041 tcggcgtaac cgattgatgt tgactgtcaa tgtattgtca tctacaaatc gttcgtcatc
     8101 ccacagtgct cgcagcaatt tttcccgact taagatcttt ccattgtgct tcatcaaaat
     8161 aaataataga cgatattcat ttttgctcaa atcaatgatc tctccgttga tttccatact
     8221 tccgttgtca acattcaacg taatgccatt gtggctcatc atttcactag ataattctgt
     8281 gtagttgtaa gaacggcgaa gtaatgcatt gatttttgca atcagtacat cgatttggaa
     8341 atgttttgtc acaaaatcat ctgcccccat gttcatagcc atgatcatat ccatattcgt
     8401 attcctgctg gagataaaaa tgatcggtac tttcgatact tcacgtattt tttgacacca
     8461 ataatagcca tccaaaacgg gaagattaat atctagtaat acaagctgtg gttcttctct
     8521 ttcaaagtct gagaacactt gattgaaatc ggtcgttcca aaggtttcaa attgccattt
     8581 ctcaagttcc tccatgatca attgtcgaat gatctcttcg tcttcgacta ccatgatttt
     8641 tgccataaaa aatccctcca tctttcttct tgctactaca atatcaaaga agcccttgtg
     8701 aaacaagttg gcttacctgg aaaatgtcat tttagcaaac gttaaaaaaa actggaaata
     8761 tgcgatttaa tcgcgtttgt ctcttccaag cagcttaaat ttattatgtt attcttaata
     8821 attggtctat tatctgttat ttttctttcg ttgtttttgt cttttaaatt ttcgataaca
     8881 ccaataaccg aaaagcgtta gcactagcaa tatacctaga aggagtataa gtaatcgata
     8941 aagatggtat ttttttgtct gttctatctt tgaagagact tcttctgtaa agggaactcg
     9001 tcttcctgtc actagtaatc ggtgagtgtt cacggtgtaa ggtgtacaag tgataagtgt
     9061 gactagatcc tgcttttctc taattgctaa tgtatttgtt tttgttggaa ggacaacaat
     9121 tttctcaacg acttcataag cgagcatccg accatacact tcaagataga atcggtcatc
     9181 ttgctccatt tcttcaagat ccgtaaagag ttttttctca gcaataccgc tatgggcggt
     9241 aatcacggaa tgtgtactct ctcctccagt tggaaaagag gtgccttgca ggactgtcgc
     9301 tcctctatct aacaaaagat cattcgtttc atcaaataaa ggcaagctaa cattgatttt
     9361 cggaatatag atggctccga tcatatgttg ttcgtaatat tcttttcctg gattttggac
     9421 atttctaacc gcttgctcaa aaggatcttt gacttgtccc attcctggga ttactgatgt
     9481 atgagcaagc gtctgatttt tcttttcatg cactgctaat tgttgctcat gtttttcttc
     9541 tttatcgcgt tcaagctgct tttgataatt ctcgatcctt ctttgatcca aataattgct
     9601 gagagcatct gctaaaaatg gatatgtaaa aactgcagct ccggcaagaa acatcactgc
     9661 cataaataat tgtaataaaa gactgatttg ggtttgaggt ctattaccta ttttcttttt
     9721 cattttcttc accttcttat ttaaaaaaac tgcagcaacc tttataaaac cgattgctgc
     9781 agcttatttt tgttgcttat acttcagact tcattttaga tttacggtac cagctgtacg
     9841 ctccaatcat caaacttatt ccgattagta agaatgccaa gagcccgttc ccaccagtag
     9901 caggaagtga cccgcgtttc atattcggta ctctttctgg ccctggattt tctactccta
     9961 cgatctgaat ttcttcatta tagcttccat aagagatgat aaattcagta aaggctgcct
    10021 cacctgttgg aagtacataa ccttctggcg ccatggtctc tttcatttga taggttccat
    10081 aatctaaccc ttgaacctca aagtatccat ttccatctgc gacaaattct gttgcttgtc
    10141 cgcttgttgc ccactcaatt ctatctgctc ctgattcata aacagctaaa ggcgactccc
    10201 ctttataaaa tattgcgtat tcagaacggt cttcattcca caattcgaat cttgcatctt
    10261 taagcaattc actgcttgat gcatcatatt tttcaaactt tcttccacct gttgtgactt
    10321 tctctggcgg ctctactcct tcttcataat caaattcatc atttcgtccg atttcaataa
    10381 ccgctgtgtt gttatggaaa tcatccgctt ctagttctgt ggaaaccttc aaattatacg
    10441 tgatcgttaa atctcctcca cgtgcatctg ctaatctacc taaagtatct actttggcta
    10501 ctccattttc ttctgtcaaa atcgtcacga caaacccgtt gcttactact tctactatat
    10561 aatcttgtcc ttttgttaaa atcgtctcgc cggctcgcac ttctatttcc tgatcataat
    10621 aagttagacc aactgtaggc gtatctgtga ttttgtaatt tttgataaca gcgcttccgt
    10681 tttcaaggac agaatcgatg aaataaggaa taggtgctgt gatcgtgtaa gaagccgttt
    10741 ttcctctttc tagatcaata taagaaatcg tttcttgttc tccagcttca ttaataatcg
    10801 ttacttgtct aaggtcgctt tcctctacat tcatctgttt atctgcttct ctgatttcgt
    10861 ttttcggata caaatgcaca tctgtattgt atgtgtcttt ccatgtagaa ccatcccaca
    10921 cgccatccac catatcaggc atcatcactg gcatcataac aaccattgga gacgcgattc
    10981 tggaaatatg agctggcgaa taagtttcta agaaaagata gactttatct ctagaagaag
    11041 gtgcttcttc cgtcacttgg acggtagaga ataccgcttc tccagcttca ttcgttgttt
    11101 gtttgtcaat acttgtcagt cgatatttta aaaaccaact ttctgtgtgc cgtttttgaa
    11161 tccattcaat cgcttcggca gaattaagac cgtcttctcg ttccggtgtt aaaggattat
    11221 ctcgaatcaa atcgtaatat acttccgtta cgtcaaacaa atcaaaatcg actcctggaa
    11281 tattttcttc accaaaatcc gtagaaacca atccgctatt cggtcgttct tccggaacag
    11341 atgagaaacc ttttttgtgt aatgtaatcg tcacttcact tggtttttgt gcgacttctt
    11401 cggctttgac tagttttccg ttcccgaaaa tccctattcc cgcagtaacg agtaacagta
    11461 cactcattcc cgtgacaatc atttttttat tcattttttt ctccccgttc tttttttatt
    11521 aataaaacag cacctaacaa taaaagaatt cctagtaaag aaatcaacgt tcttgcttca
    11581 ttcgtttgcg ggaatacgga ccattttcct gattgacctg gattgcttgg accactcggc
    11641 tctttcggtt tgtccgttgt ttcttttacg gtctcgttat aaacacgtgg tgttgcggat
    11701 tcataggcag agactggaac tttcccggat tctaattcag ccatctcctg tccgttaact
    11761 actacatatt gtggttggcc ttttgcatcc acccaaaatt cagggatgat gacctcgatt
    11821 ctcttacttg cttcatccat ctcgtaacct tccacacctt ttaattcttc aaaataatag
    11881 gtgccagaag gcaagaaacg ttgtcctgtc gtaacaaggc cctctttatc cgaaatgaat
    11941 ttgcttacat ttttgtcatt caaaggatca ttgtctgctg gtttgatcca ttgatttttc
    12001 aagtcattag caggagccat atctaaataa tatttccccc cttctatcat ctgatataga
    12061 gcaaaaacag caccttctaa tggttttcct ttttcagttg ttcctctttg acgtccatac
    12121 ttgaagaagt atggatctct tagataaccg acatttttag gatagatatg aatctcttgc
    12181 agttcctcgg ttgattctgt cgggtgataa attggcaaaa tagcagcaat tgggatagct
    12241 gtctgatcga tatcaacatc caatccaact tctggatcaa tcgattcttc gaaaatcaaa
    12301 taaactgcat cccgatcttg gtttttctta ggtgctttga tacgtgcaat cccgtctcct
    12361 aatccgccaa ccgtatcttt cccagttgtt accgaagtcc ctatttggac aagcctttct
    12421 tctgtgatta gctgacggat attgtttctg ttcgtttcag cgatttgctg gacgaattct
    12481 ttaggagtca tgttttcagc agcttttttt tcatgataat aatcggtcat gtcgtaaatg
    12541 gcaaatgtcg ctccatttag tggcaccgtc tgtgaaatga tatctgctgt ttcccctgct
    12601 tcctcagaga tagccaagcc atcattttca tggtattcaa attgatcgtt ataatcgata
    12661 tctcgaatca tccgtttatg caacacgaat tccacgacat cttcccctga ttcttctgcc
    12721 gacatcgcat gtggaagaag gactgctgca agcggtagta aaacaagtaa ttgaagcatc
    12781 aaacacaaca gctttttcat taccttgctc cctccttttt agattgtctg acaaacaaac
    12841 cataggctgc tcctgcaagc gccatgaaga atgcagcaag caggatatag aataatcgtc
    12901 cggatcctcc tgtagcaggc aacggatttt caggatcgtt cccgacacta agctgtaatc
    12961 tgattcgatt tgtctccaaa agttcatatt ctaactcttg ctgtagatca ggcgtctctg
    13021 cttcttcgtt tggataatac gaaatctcaa cttttagtgt acccgcttct tttcctggaa
    13081 ctacccgatc attttctatg tcaggttctt ctcgataact aatttctaaa ataaagtaac
    13141 ctggaagttt tctatagctg tcaggtgact tcacttcctt gatccgatag accccttcca
    13201 acaagtctga agcattgaat agggaagaat cgtttggatc tggtgccaag cctccctcga
    13261 tgatttcata ctctcctgca ctattttgtt tctcaattga aaattctgcg ccttcaagac
    13321 gtcggctatt catactgtca attttcaaaa gttccaaatc gattggcgct agttcatctt
    13381 ctaaggttac ttgtagtaca ccatcgatga tttcatagcc ttccggcaag ggagcagttt
    13441 cgtcttcgat gaccgtatct tcaatgataa acttaccttc ttcattcagt gcaaactgga
    13501 acacttgatc agatagccga tacccatttg gtgcgacaga ttccgagacc tcatattcac
    13561 caatagacag atgcagaggt tggtcgtaat ttaaatcgac atacttttct actcctacgg
    13621 cacgagaata gaagtatccg atcccaggca aacgtttcga ttcttctggt tctaacggat
    13681 taccagtgta tgctcgatac agcgggaagg aagaatcccc cactttcctg atatcgaaac
    13741 ctgcaccatc taatgtatgg ccttcatggt ctaattttct aactcggatc tcccgataat
    13801 caaacgtatt gtaagcagtg aatgctaccc ataaacgatc ctcttcatct gttgtatacg
    13861 tgagatattg ccaattttct tcacctgttt gcttgagttg gatagtccct atagcattat
    13921 cgtaaagtcg caagatccat gtgtcatttt catcaggttc tttgaaacct tctgggttct
    13981 ggctctcaat gattttgtat tctgcatagt tggtagtttc ttcactatcc ttataaacaa
    14041 atgaatctgg ctctccgatt ggatgaataa ctttctcacc atccacgatt acatatttcc
    14101 cgtcttcatc tactgaatag aacgtgagtc tccctttctc ttcctctact tcgttcacac
    14161 cagaaagccc tttcgctact ggatatccga tttccagtcc gttaccatct gtttcataaa
    14221 gttcaaactc tgcgccattg attggcatat tactgccttc aattagttta ttgatattca
    14281 tgtggattgg cttgaattca tttcgcattt ccagtttgac atacaactca tcatactctg
    14341 tgccttctat gttaggctct tgtacaacag aaagcttgtg atattctcct tcataatagg
    14401 aattcggatc tttaccttcc ggtccgccga ctctcgaacc agatgtatat tcatctacaa
    14461 gctcgtagtg acctaacatc tcatcttcgt tggctgcata ttttttgatt tggaattgat
    14521 acgtctcatc tactaactga taacgtggtg gtccagcgat ctctctgatc caaaatatgt
    14581 cgctgactgc tccagtttca tctcctaaaa tcggcatctt cgtttcaaag atcgctattc
    14641 cgtccgtggt agttttactt gtttcgattg gttcggcttc cccactctct ggattttggt
    14701 catataatgc aaattcgact ccagccaacg gctcgccatt ttgacccttc ttgctgactt
    14761 ggaaattacg gcctttatgt tcgtaacgaa tggaagaaga tgggaaatac atccgtccag
    14821 tagtgatatc attctcataa tcctccaggt tctgaatata ggccatattg ttcactgtgc
    14881 aaaaatttcc gtcttgagct cccgcatcaa gttttacttg gtacttgatc ctgaccattt
    14941 ccccttcacc taaagaaatc ggagaaaatt tataagcttg gttttcccgt ccgacatacg
    15001 ttgtcggagt cgccactact tctggtgggg cttgtccatg acgttcttca tcccatattt
    15061 ctggctcttg gtacttcatg ccgttttctc ctggatctag atggtaaaac tgaatatctg
    15121 ccgatctgcc agctccaaat ggtgagacat taaaaccagt ttcatcataa agtgtggctt
    15181 gctggatcgt tctcttgaag gtagaagaga tatcctccaa ggctgtaaca atgtcattct
    15241 tgttgttcgc attgtaatat cgcggtgtta cgccatcttc ttctaaatca gcaatatttt
    15301 tcaatacatt tcgtccaata gcatctagtc gttgtctccc gctaacgttt ccatcgattc
    15361 ctaaaccaat ggagaagata cttggcgggt tctcttgata cttgtttttc agccaatgat
    15421 gataagcaac tgtgttgatc gcagatgact tttcttgcga agtaggactg ccattcgtat
    15481 tgttattaga agaaccatca ccaacccagt gagtcgcttt attatctcct tcttttacct
    15541 ctccataacg atagtgtaca tttacttcat cttctggacg gttaaagtca gatgcatagg
    15601 taactggatg agtaaaccct gcattagaag tattcccaga aaactcctca aaatttctga
    15661 acaaaacgtc attatcttca gcaatcttat cttccatcac agtccagttg ctccaagctc
    15721 cattagtgct cgtacgataa cttgactgaa tcggtgcata actgaaggtc ggtgtcccat
    15781 ctcccacaac gattaggatt ttttctggat ttctattttc tccgccattg tctttataca
    15841 atgtttcata gccattttta agccctagtg taagcggagt tcctccgatt ggtgtacgat
    15901 ttaatgctgt attctcactt cccgtcaaac gattgatatc atttgtcaat ggaaagatgt
    15961 ccgaactgat ttgctcatgg ttattgatag ctgtactatt atggtaaaaa ttcaccatac
    16021 cgattcgaat atccagactc gggtcagaga gcagatcttt actcatttcg tttactgcct
    16081 caatcaacgc agcattttta gtttgactat tggtcccagc ggttaattca ctcatactcg
    16141 ctgacttatc tagaacaaaa acgatatcga ttggactgat ttcctttttc tcgcccccga
    16201 tgacatctag atagacattg taaagggttg ttgcatcttc aggatgtaga tcagatgctg
    16261 tgctgcttgg cagaacaggt tctacccatt ttttgatgta cgccccgtta taatccaagt
    16321 aactgttttc aaaattccgt tcatttcccc aaagctgaac ggtttgagct ggaacgtttc
    16381 tatcttcttc gcctgcagtt gatccattat tagtagcata gtcgtaattt cgccagttcg
    16441 ctcccccgtt tgtataatca ctatcatcga tcaagttctc tccgtcatat ggcgagcctt
    16501 tgtctccttg atagcgttca ttgctttgtg tccaatttcc taacactttt gtatagtcat
    16561 tcgttgtaaa cttcgggtag cgatgctgtt cgttttcaac ttctccgcta ggatagatat
    16621 attcataggg gtcttgcgta gctgttggcc caagacgctc caaaataccg ttgaataact
    16681 ggcgttgaat gcgctggagt gcttgaccgc tgggtacgat tggttcgtcc agcttttcca
    16741 tctcattttc ttcgctacgt tgaatttcag cggaatcttc tgtatcagtt tcattttctt
    16801 gtaattcttg gttatctatt ctttcatcaa ccaccacttg aagaggtagt tacccttcgt
    16861 tcacatcggg gatcttttct gtttcaaaaa tcaatgtgcc ggtttctgct tcttttgaat
    16921 aaacttttcc gacataccat tcttgaccat cgatcgtctc gacttttagg tcttctccat
    16981 cttcatcata ggaggataga cctgttccac gaacgttttt tacggtacca atcccggcgg
    17041 ctgccgcatc gattgctaaa cgtattctac cttcattggc actttccttt ttttcaaaag
    17101 ccagatgcca ttcaagggtt tcttcttttt caatatactt cccttcaatt tttgcatagt
    17161 cctcatctaa gaatacctca tcctcctttg tttctgttga ctcagcagct acatccaggg
    17221 tcgtcatatg atagactgac tggaaatatg gtccaaatag caaactgaca gttactaact
    17281 gcaaaagctt caattttaac cctttcacat ccttatactc ctttcaaaaa tatttgtctt
    17341 ctttcacaac aatcaacaaa acaatgccc
//
LOCUS       DAZKNH010000053        16747 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000053 DAZKNH010000000
VERSION     DAZKNH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.053"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..729)
                     /locus_tag="ACG8MG_001677"
     CDS             complement(16..729)
                     /locus_tag="ACG8MG_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0375720.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            904..2328
                     /locus_tag="ACG8MG_001678"
     CDS             904..2328
                     /locus_tag="ACG8MG_001678"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0375721.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2627..3334
                     /locus_tag="ACG8MG_001679"
     CDS             2627..3334
                     /locus_tag="ACG8MG_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0375722.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3321..3644
                     /locus_tag="ACG8MG_001680"
     CDS             3321..3644
                     /locus_tag="ACG8MG_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0375723.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3733..4770)
                     /locus_tag="ACG8MG_001681"
     CDS             complement(3733..4770)
                     /locus_tag="ACG8MG_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0375724.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5091..6074)
                     /locus_tag="ACG8MG_001682"
     CDS             complement(5091..6074)
                     /locus_tag="ACG8MG_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFQ0375725.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6489..7259)
                     /locus_tag="ACG8MG_001683"
     CDS             complement(6489..7259)
                     /locus_tag="ACG8MG_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0375726.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7252..8187)
                     /locus_tag="ACG8MG_001684"
     CDS             complement(7252..8187)
                     /locus_tag="ACG8MG_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375727.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8503..10152)
                     /locus_tag="ACG8MG_001685"
     CDS             complement(8503..10152)
                     /locus_tag="ACG8MG_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0375728.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10269..12872)
                     /locus_tag="ACG8MG_001686"
     CDS             complement(10269..12872)
                     /locus_tag="ACG8MG_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0375729.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12884..13933)
                     /locus_tag="ACG8MG_001687"
     CDS             complement(12884..13933)
                     /locus_tag="ACG8MG_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0375730.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13959..14273)
                     /locus_tag="ACG8MG_001688"
     CDS             complement(13959..14273)
                     /locus_tag="ACG8MG_001688"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFQ0375731.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14289..14735)
                     /locus_tag="ACG8MG_001689"
     CDS             complement(14289..14735)
                     /locus_tag="ACG8MG_001689"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFQ0375732.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14736..16658)
                     /locus_tag="ACG8MG_001690"
     CDS             complement(14736..16658)
                     /locus_tag="ACG8MG_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0375733.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT         4962 a         3407 c         2759 g         5619 t
ORIGIN      
        1 ataattttcc cgagcttatt tttcaacaaa aacagcttca aaaacaaagt cttgatataa
       61 atgtctcgat accgaatact cgaaaggttt accagacgat agagcaacaa ctttgtctat
      121 ctcaatcata aattcacctg gatctatttt taatattttt gcgtcaaatt cattcgcagt
      181 atctcctttg atacgtatgt gcgaagtctt tatttccaga cccagttcat ttgtgatata
      241 agcatataca gatttcttta aatgttttgg ttccaagcct ggtatgactg ctaatggcat
      301 gaatgtttgc tcaatcgagt atggttgaca atcaataaaa cggcttcgta caatattata
      361 tacatagtct tttacactga tacttaattt atcagctact tttttcgatg gaagttcaat
      421 tgagaaagta tggatttctg aatctacgtg ttcttcacca accacgctaa acaaaccttc
      481 gtttggacca agaactcgtg agttatcaat tggtgttctg acgacttcag tcccataccc
      541 acttctactt cgcaaaactc cttcggaaac aagtaagtct acgcctttct ttactgttaa
      601 cttgcttgta tgataaataa ctgccaattc atctcctgta ggaagtttct caccaatttt
      661 atactctcca tttacaattt ttttctttat atctaagtaa atttccatat acttcgcaat
      721 ttttttcaat ggtgaccctc caatcaaaaa catatcttca tctatacttt aacataaata
      781 aaccgataaa atccacatca aaattataca cacaataaaa aagatatata tatttaaaca
      841 aaactattta ctttaagttg taagcggtgt tatcattaag tggaaacagg aggtaaaaaa
      901 attatgacaa ctatgaaaaa gctaactttt ccagatgatt tttggtgggg ttccgcttgg
      961 tcagctgagc aagcagaagg aaaaggagaa acaggaaaag cagagactat ctgggatcgc
     1021 tggtttaaag aacaacctta ccggttttat caacgaatcg attcctctat tacaaccgat
     1081 cacatccatc aatataaaga agatatagaa ctaatgaaaa aaactgggca taactccttt
     1141 cgcgtttcta tttcttgggc acgaatgttt ccaaatggcg gtaccggaaa agtaaatccc
     1201 aaagccattt ctttctatcg aaaattattt tccgaaatga ataataacgg aatcaaagta
     1261 tttgccaact tatatcattt tgacatgcct gctactcttc aagataaagg aggatgggaa
     1321 tcacgtgaag tagttgatgc ttatgttcat tacgctgaaa cttgttttaa agaatttggt
     1381 gacttagttt gtcattggtt taccttcaat gaaccacttg ggcctatttt aggtacttat
     1441 cttgaagatt ttcattatcc aaacctcatt gactttaaac gcggagcgca agcagcattc
     1501 ttcactatac ttgcacacgc aaaagcaata caagtattta aagaactagc tcttcctagt
     1561 aaaataggcg ttattctaaa tttgagtcct acttttcctc gtagtcaaaa tcctgctgat
     1621 gtgaaagcag cagaaatcgc tgatttattt tatacgcgga gctttttaga tcctatggtt
     1681 aaagggaatt tttctaaaaa gcttatcctg ttattacaag aatataatca gcttcctgaa
     1741 gattatacga aagaagatct tgtatgtatc tacgaaaata ccgctcaaat tcttggacta
     1801 aactattacg agccacgtcg cgtcaaatct cgttcttcta tggtggatac atctagtcca
     1861 tttttaccag agtggttttt cgaaccacat atcatgcctg gacgacgaat gaacgaatac
     1921 cggggatggg aaatttacga aaaaggaatt tatgatttgt gcatggatat caagaaccat
     1981 tatggaaaca tcgaagcatt tatctcggag aacggaatgg gcgttgcgga tgaagaacgt
     2041 ttcatggatg atacagggca aataattgat gattatcgga ttgagtatat caaagatcat
     2101 cttgcttact tatggaaggc aataaatgaa ggatgtaaca ttaaggggta tcatctttgg
     2161 acatttattg attgctggtc atggatcaac gcctataaaa accgatatgg gctggtttca
     2221 ctcgacctta ctactcaaaa aagaacaatt aaaaaaagtg gcgaatttta caagcaatta
     2281 agtgatacaa atggatttag atacgatgta gacttgttag ttgaataata ttctaaaatt
     2341 ccacttgaag tgaacacatt agaaaacaag aataatgaga aataccaata acaaatactt
     2401 tttcttcata cttcatttat acttgtcagt ataagagact gtgacaaatg tagtgatcct
     2461 cagaagttag gtctggaaat ctaacttgag aagatttttt taaactacgt ccagtctttt
     2521 ttatttgttt aaaattgaag ttgacaagtc ttcattaaaa atttagaata ttcacaagtt
     2581 tgacgacaat tatgaaaatt tatgtcgtgc taaaggagtt ttgttattgg atgaaaagtt
     2641 agatataaaa actgcgatca aagacacgtt acctaccgtt tttggttata tcggtattgg
     2701 acttgcattt ggtatcgttg ggaaagctgc cgggtttcat ccgctggtcg tcacgttgat
     2761 gtccctgctg atctatgctg gttctgccca atttatcacg gtcagcatgc ttgctagcca
     2821 tagtccactg ctctccatcg ttctctcgac ctttctagtc aattcccgaa tgattctgat
     2881 gagtatgacg attgctcctt atttcaagaa aaatcggctg cttcaaaatc tgctgattgg
     2941 cacattgctg acagatgaaa gtttcgcact gggaatgaat aaattgaact atactggaca
     3001 aaagttaaac tttcgttgga tgaatacagc taattggatt tcttatttga cgtgggtggg
     3061 ttcttctctt gttggcgcac ttttagggaa ttttattact gatccgaaaa agtttggttt
     3121 ggatttcgcc atcgtcgcta tgtttatcgg gttactttat cttcaagtca tttctgatag
     3181 aaacacctct aaacgattac agttgatcct gattggtctc actttgattc tggtatatgt
     3241 cggactgatt tttatcccaa gtaacttagt gattttagtt gtaacactga ttgggtgcgg
     3301 attgggggtg tggatcaaac atgccttctt ttgagtttac attgctgact attttaggtt
     3361 gtactttagc aacttggctt tctaggatcc taccatttgt cttactgaaa aaattcgatt
     3421 tgccgcaacc gttgcttgag tatttaagtt ttgtcccgat cgtcattatg tctgctttat
     3481 ggttcagcag tctatttacc caaaatatcg gacatctgcc gcagatcaat ctcgaaaatg
     3541 ctttggcttc agttcctaca cttttagctg ctattctttc aaaaagctta ttggtgatcg
     3601 tcttggtagg aattctttcc ctttcgttga ttcgcttatt ataaataaaa aaacaagagc
     3661 ttatggcaaa ttactgtcat gcgctcttgt ttttatcaaa gtcaaataag aaaaaaatcg
     3721 gcgtattctt gcttatccgt tgaacggacc actaacaacg acttttccta atatatgtcc
     3781 tgtttctacg tctttggttg cttgtttcaa gttcttcgca ttcatcccat ctgaatagac
     3841 cttgctcaat gtactgcgga taatcccttc atttgctaaa gcagcaatgt gcgctaaagc
     3901 ttctccttgc gtttcgattt catagttata gtctgtttta gcgaacatat actcccagtc
     3961 gaagctgata gattggtttt tccaaacacc tatatctaac ggctctttga tccctacgat
     4021 cgttcccaca tgtccaaatg gtcggacaag gtgtttgatt tgatcaaagt agcgggtaat
     4081 atcgaataat acagcgacat actctacttg atcgattcct aattcacgaa aacttttact
     4141 taagtcgtga tgataatcga ttggatagtc tacacctgtt tttttcagcc attcaaaatt
     4201 cttcggactt gcagtcgcgt agacttctag gccagcccaa tgcgctagtt gattcaaaat
     4261 cgaaccgacg cccccagctc cgttgatcac taagattttt ttcccgcggt tggcattttc
     4321 ttctggagtc agaccaaatt tttcaaataa caattcataa gcggtcaatg aggtcaacgg
     4381 caaggctgcg gcttcttcat ctgaaagatt ttttggtgca agagcgacaa tacgttcgtc
     4441 aaccagttgg tattcttgat tgcttcctgc tcttgtcgtt gtacctgcgt aaaaaacgcg
     4501 atcgccgaca gaaaattttt gcacttcatc acctacagca acaactttcc cgactccatc
     4561 aaaccctaag atacgtaaag catttcttat cccgttatct tggcgaagtt tcgtatcgac
     4621 aggattaaca gatactgcgt tgacttttac cagaagatcc cgtgaactgg gtacaggtat
     4681 ctgttctttt tcatcaataa agctttttgg atcatcgatc gacaaccctt cataaaaacc
     4741 gactaccttc attaattcct tttctgacat acgaattcct ccttagcttc atcaagttgg
     4801 agcatccata cagttttcat ttcaatatat atagtatgct atttgttcca cttttattca
     4861 tcataattat tctcttactt aaagtaaact atttgcactt ttttggttaa aaaagaggtt
     4921 gcgacaagac ttttgtcaca acctctttta aacggtgttc aatcaactga ccctcggtat
     4981 tcgctcaaaa aacgacaaaa tatgaggagc tatttttgtt tggctgttct tattacttga
     5041 tgcaggacag ctttttcacg acctctaatt cacggtgttc aagcaagaaa tcattgttca
     5101 ctgaaaagcg ttggatgttc tttttcaaag gtgtctaaga tctctcgtac ttcatcagta
     5161 gatttcgtat tcatcaactg aactcgcaag tcatttgctc ctggaaatcc tttgatatag
     5221 attttaaaga aacgatgtag tcctgtgatg gaacgcggga tgatttctga ataatgatct
     5281 tgcagatcta gttgcagacg aagtagtccg atcagttctt tttctgtatg ttccctcggt
     5341 tctttttcaa atgcataagg attcttgaaa atcccccggc caatcatgat gccatctacg
     5401 ccatattttt cggccaattc ttctcctgtc tggcgatctg ggatatctcc gttgatcgtg
     5461 atcagtgttt gcggagcgat ttggtcacgt aaagcaatga ttttagggat gacttcccaa
     5521 tgtgcatcta ctttactcat ctctttacgc gtacgcaaat ggacagaaag attggctata
     5581 tcttgatgaa gtaggtgact gatccattct tccatctcag acatttccgt atagccgata
     5641 cgcgtcttga cgctgacagg caaaccgcca gctttagccg cttcaatcaa ttgtgccgct
     5701 acttctgggc gaaggatcaa gccactgcct tttccacgtt ctgctacatt agggactggg
     5761 cagcccatgt tgatatcgat tccttggaag cccatttctg ccatagcaat gctcatttct
     5821 ctaaaaaatt ctggcttgtc tccccaaata tgggcaacca tcggctgttc atcttctgta
     5881 aaggtcaatc ttcctcgtac actgtctttt ccatctggat gacagaaact gtctgagttt
     5941 gtaaactcag taaagaagac atccggtgct gctgcatgct ttacgacatg gcgaaataca
     6001 acatctgtta catcttccat tggtgctaaa acaaaaaaag gctttggcag ctctttccaa
     6061 aaattagtag tcatttattt gttactctcc attctatcaa gcagtgaagt cgcaatcttt
     6121 ctcaaaaaca ttgtcattat actaggaaac aaaaaaagaa gcaaagtgaa cagcttaaaa
     6181 gatgtttctg attgagcaaa aaaagactgt gacaaaaatc ttatcacaat tccttttccg
     6241 gaataaatgg tgttcaaaag cttgcatctg cggtaataag tccaaccaaa cgaaaaatag
     6301 gaggagctat ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc
     6361 tctccactaa acggtgttca atcagctgac cctcggtatt agctcaacaa acgacaaaat
     6421 atgaggaact attttcccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa
     6481 cctcttttct aacttctcaa tcctcttcct ttattgatca aataccaagt aacggcgtac
     6541 aaaacaacga tgaatagcac aagtacgatc atcgatgttg ctactggcac atcgattgtt
     6601 ccaaggaatc cgtaacggaa accggagatc atataaacga tcggattgac tttcgaaaca
     6661 gcttgccaaa atggcggcaa catagagatg gcatagaaaa cgccacctag ataagttaac
     6721 ggctgcaaga caaatgtagg cacgatcgat acatcgtcat aagactgagc aaaaatcccg
     6781 ttgagcagtc ctgctaacga aaacaagatt gccgtcatca ataatgtaat gatgaccatg
     6841 aaccatgaat acacatgaag tggtacaaag aacaaggaaa tgatcgttac gagagagccg
     6901 acgagaatac tgcgacccac tccgccaatc acaaagcccc aaatgatcac atgcgtagga
     6961 acaggcgcta ccaagatttc ttcgatgttt ttctggaatt tttgagagaa gaaggacgaa
     7021 gaaacgttgg catacgaact agtgatagca gacatcataa tcaaaccagg cacgatgaat
     7081 tccatatatg aaaaaccacc catgtcaccg atccgaccac cgatcatttt tccgaaaata
     7141 acaaagtaca aggatgtcgt gatcacaggc ggaaccagtg tttggaccca gatacgcaaa
     7201 tagcggttcg tttccttgat agctaaactt tttaatgcag taaaataaag attaaacatt
     7261 tttctcctcc gtatgattat tttcttctgt gattttcaaa aacagttctt ctaaacgatt
     7321 cgacttattc ctcatagaaa gaactttgat tccttgtctt gtcagctgat cgaagattcc
     7381 gttcacccct tgatttcgtt caacttcgac agcgattgtc tgttcatctt caaatgtaca
     7441 agcgtatcct tcaatcacag gtttggtctc atatggcgct aaatcaaaga taaatgtttc
     7501 aaattgcagt ttggatagaa gctctttcat actagtattc tcgatcagtt cacctgattg
     7561 gatgatcccg atgttgcgac aaagcatttc agcttcttct aaataatgcg tcgtcaaaat
     7621 gatcgttgta ccattttcgt tcaattctcg gagaaactcc cacatctcgc gtctcaattc
     7681 aatatcgact cctgctgttg gttcatccaa gatcagcaaa cgtggttcat gcatcaatgc
     7741 cctagcaatc atcagtcttc gcttcattcc cccggaaagc atacgtgcac ggacatttct
     7801 cttttcccaa agattggact gcttcaaata tttttcgctg cgtttcatcg cttctttgcg
     7861 agagacaccg tagtagcctg cttgattcac gacgatttgc tggaccgttt caaagggatt
     7921 gaaattgaac tcttgcggga ctaatccaat ctgttgcttt gctcggacta gatccgtatc
     7981 cagatcataa ccgaagacat tcacttttcc agatgtttta ttgactaaag aagtaatgat
     8041 tccgatcgtt gtagacttcc cagctccatt tggtcctaat agcgcataga aatctccttc
     8101 tttcacggaa agatcgattc ctcgcaatgc ttcgacccca cttccataaa cctttttcaa
     8161 attatttatt tcaagcgcat ttgccattta tttcctatct ccttatagac atgattcttt
     8221 attgtatcat ggaatacagc agatgaaaaa acaacttggt tccagcgaaa aatcaggttg
     8281 ctgggctcag gaaaatctcc taatgaaaga catcaatcgg acgaagtcag acaacctttt
     8341 cagaaaaaac aaatcgaaga taaaggatta ctattcatat gtgcaacaaa aggagcgtga
     8401 gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaagcaaa
     8461 agccaagatt tttgatccaa taaatacttt cgatcacttc gcctatccca atacatgcca
     8521 ctgccgcttt tcatcaaata acgcggcttc cgtttttgcg gtttctacta aagtctctgg
     8581 gtaatccaaa actttcagca aggcaatcgc atttcgggtc cttgaaggtc cttcttttaa
     8641 tttgaaatca aaactgattc cttttccttc tgttacttgt tcttcaaagt ggacattctc
     8701 acaataattc tttaaaatct ctgtcaattc gatatcatgt gtggcgacaa atgctaaaga
     8761 tggatactcc gcaagccacc gaacgactga agcggaagaa gcaatccgtt cgatcgtatt
     8821 tgtccctttt aaaatctcat ctacgaagca gtagcagcgt tctttggaag ccacttggtc
     8881 caacaaacgt ttgatcgatt tgatttctga aacaaaataa ctgtcgcctt caaataaatc
     8941 atcttcgatt gccatggaag tcaacacatg tccaggttgc agggtaaact gttctgcaat
     9001 cgctgtttgg atcgtctgtg ctagaataca gctgatggct atacttttga catacgtaga
     9061 ttttcctgat gcattggaac ccgtgactaa tgtattcttc tgccagttga ccggattcac
     9121 taccgcttct tttaacaatg gatgatagat atctttcgct gtcacaccgc cctcagtaaa
     9181 ctctggtcgg caagtgatgg gcatataagt acggtaattc aatattgcag cggctacttc
     9241 taattctccc agcaattccc acagcttgat ggcttcagtt gaatgctttt ctaatttagt
     9301 gagaacgaaa tgataggaga taaatggcag catgaacatg atgttgacat attcaaaaat
     9361 caactctgct tcactcccac tcttcatacg aaatgaaaaa cctaatttag ggatgtctct
     9421 taatggttta gtttgctgtt tcagctcttc ttgaagtggt gtcggcagtt ttgctagctg
     9481 attggcggct gaaatcgttg aaaccaaata actcatgcta ttcagctctg tttctagtgt
     9541 ttgtttcttc actagataat agaccgtatt gaatactaac ccaccgattg ccaaatagat
     9601 tcctagtact tgtcctaaaa gaagcaaacc caaacccacc agcggaagca agccaagaaa
     9661 aataaataaa cctacgcgtc caatcttttt actttttcca tccgctagat attgttttgc
     9721 aaaattatga tctttttttc ctaaacgagc gaattgatat tggatttttt ctctcagttg
     9781 aggattttcc tgataaaaag caatcagttt ttctaactgc tgatcttcac caaaatcaaa
     9841 gctgcgtaaa cgttgataca acgcttctga acctacacta gaataggtac tgttgattcc
     9901 ttcgaatact tcgaacatat ccaggtcata ccaagtaaga tcatcgattt cgctatccca
     9961 tgaacggaac ttcttctccg tgagccatgc ttccttcaag ctttcttctt tatcaaatct
    10021 cggttgataa ggaatttttt cccatttgct acgaactgtt tcctttaatt tcaaacgtga
    10081 aaaaatgtct aaagcaacta ataaaataac agctgcaata ataattaaaa cgataaattc
    10141 tctttgaccc atatccctct ccgttccttt atttgttagc tttagtatag cactgttgcg
    10201 ctatttgaaa agagggtaaa aaaacacctt tcaaaataaa aggtgttttt ttcttgtata
    10261 ttacatattt atctctcatt tatcaaaatt gacaaataat catagggagg aattgttgtt
    10321 aattcttgtt tattctccag tgcattgaca actgtcccaa gggaaaggat agcctcaggt
    10381 actatttttt cttgttctgt tggattagct aagcgaagaa caaatccttg ttcatgaaaa
    10441 taagaaggat aaatcgatga tacaattaag tcttcaggta attcaatcaa tgaaaattta
    10501 cgaggaacat caagttcatt ttgtaatggc caaatcttgt tatctaacct gtgaataaat
    10561 acattcagtt tctgcttttg ataagaaata gattgttcta accgtttttc tgcctgtttt
    10621 gccactttga actcattaaa tcttccttct tctacattaa atccaaaagt gactttatac
    10681 tcacctatct cttgagctag cggtgtttcc atcatgacat gcccttcatt tgtcgtatct
    10741 cctgaagctc gcccaggtcg ccaagcaaga ttaggttttc caagttcccc tgttgttgaa
    10801 aataaggtta tgattaattg attttctata cgttcatact cttttaaacc atctgcataa
    10861 aacgtaaaat aatgatcatt ttctgccaca gagacactct tatcgaatat ttctaaatta
    10921 acaggtttct ctaccatagt ttctgaccaa ttcggaggaa tgacatcagg ggtattttca
    10981 ataaatccgt tttgaatttg tgcaattgat ttttcagatt tatctaaaac attaattgct
    11041 aagcgcatac gatgcgagta aacagtatta tgaattttga gtgtgccttc aacgatttga
    11101 ctttctccca gactgaacga tatttcataa gatatttctt ccatttcggg tttttcagat
    11161 aaacgatctt ttaaatcttt aggaagttga gcttttccgt aaacaactaa tgtttcttgt
    11221 aaagaatctt tatatacttt agctgtttct aattttagag atagttctgt atctccttct
    11281 aatggtgaat aatcatacgt atcgccatca tttgctgaat cgatcaaatg aacgaaatca
    11341 agatattgtc tatctccaac tatcaaattc aattgcccat ctttaaaaga aagtttatat
    11401 ttattgttac ttatctcagt tttgtctatc tcgattgctg tttctaattt ttcattattt
    11461 ttctcaaatt caacaacagt gtatcctagt gctggcacat ttacatttat ttttatgtca
    11521 agttcataat aagctggttc atcaaaatat tcgaaacctg tagcaatcat ccgataagca
    11581 tttttccgtt ctggaaaata aacttctttt tcaatgatat tatagggact acctttgaag
    11641 tgtattttct ttgaatcagc tactacatgc acgattttcg atccactata attctcagtc
    11701 gttgtgttga aaacaaggac ttcattcgat tttagcttta atgcatcagc catccgtttt
    11761 acaataaggt tttcgattcc gtcagctatc tcattagctt ctttgattcg atggaaaata
    11821 tcttccgcaa cattatctga aacacagcca ccaatactat catgagcctg gttttgcaaa
    11881 atctttttcc ataaccgacg aagtaggcct ttactgatat ggataccatt tctttctgca
    11941 atcagcatga gcggctcaac tcgttttaga attttttgtt ccaatttaaa attttctatt
    12001 ttcatttttg ttcttactga gccaatagaa cgatgaacac gagagtaaac cggctgacga
    12061 agttcacctt tatattcagg taattctttt ttgtttctga taacttctac ataagaagga
    12121 taatctgaaa taatattgtg gaatttactg tatttattca acttctctac gacttgatca
    12181 aaatttaaaa tcattgactt ttgatctaca cccgaaggta ctaaaatatc tttgtttttt
    12241 ccttgcatat gaagcatttc aactgcttca tccaactttt cttttgcaaa ttcaggaata
    12301 tctttaattg cgtctaaggt catcattcca gtcatataac caaatggaaa attcatggca
    12361 taaacaaatt tatctcccaa tcctttccag ataaaataag gagatggaac catcgtttca
    12421 aagtttaatc ctcgccaaca catgaaatta tcgatttcta cttgatgcaa aagtgtaggc
    12481 aaattcgcat tgaacccaaa cgtgtcaggc aaatacccta ccatcattgg atctccatat
    12541 ttaaggatcg tatcactgat tccaatcatt aagtttctta aaattgattc agcatccact
    12601 aataaggcat ctgtttgtgc ataccacggt cccacgaata gttgtcctgt tttcatcagt
    12661 cgttggataa ccttaagctt ttgcggattg atctccaaat actcatcgac gattgaagtt
    12721 tgtccatcta aacaaaagtt tactttaggg ttcctttcca attcatctag aacttcagta
    12781 aagacctgat cagacaatac gatggcatct tcatttgtaa aataccattc tctgtcccag
    12841 tgcgtgtgtt gaattatatg tgcatttacc atatcagatt ctcctacttt ccttcttcta
    12901 ctgaatttac ttgttgttct tttttatact tgatggattt tagtgcattg accattaacg
    12961 ctgtaaccac tcccccaata acaattgata ctaaatataa aggtatattc gtaacaaaag
    13021 gaattgccat aaaggttgaa agcattgttt cttgagtaac accaaaaaat ccatttagac
    13081 cacatgctac tgcagtgccc accacgatgc ttggtattac tcgtaaagga tctttcacgg
    13141 caaacgggat cgtcccttca gtaatcccca caaggcccat aatcatagct gtctttgcat
    13201 tatctcgttc cgattcatca taaagttttt tgtttaaaat tgttgctaaa aacattccaa
    13261 atggcggaat agcaattcct aataacatgg ctgtactagg tccaaagctt ccgcttgaaa
    13321 aagaagcaac cccaaaagca tatgcgattt tattgatagg tcctcccata tcaaacgcca
    13381 tcattcctcc taaaatagcc gacatcaaag cagcatttgc tcctgacatg ccttctagcc
    13441 aattagtcag tcctgttgtc attgcttgaa taggcgtagc tagtacatac cataatatga
    13501 ttccaacaac taatgtagta acaaaaggaa tcagcataat tgattttaga ctaataactt
    13561 gcatcggtac ttttactttt ttcaatgcat tagctaaata gccacaagct aatccagcaa
    13621 ccattcctcc aataaatccc ataccaagat tttgacacat catcccagtg ataaaagctg
    13681 gtgctagacc gggacggtca gcaattgaat atgcgatata ggctgaaatc atcggtacga
    13741 taaactgtaa tcccgtctga ccaatcattc gcaggactcc ccagaatgta tccgtctggt
    13801 cccaaacacc tgtgccacca aaaatgactg caatagaagt tagtaaccct gctgctacga
    13861 caagcgggat catataggaa accccacttt ggaaatgatt tagaatttct cttccttgtt
    13921 tcttgaattc cattagtaac tcccccacga tatatttttt atttgttaat taatgcaatt
    13981 gcttcatcaa tcgtctcttc accatctttt actacctctg aggtccctac tgttaaaatc
    14041 ggtttaccgt caaagcgacc tcgttctgaa acatttgtat ctactgcaaa tatgacaaca
    14101 tctgcctcgg aaacatccga aattgaaagt ttgttttcta tccccatagc cccctgtgtt
    14161 tctactttaa tatctaaccc tttcttcttc gcaaactttt ctaaattagc ttttgccata
    14221 taagtatggg caatccctgc gatacatgct gttactgcta ctatttttgt catatgaaat
    14281 tcctcctttt aaaatgatag agcttttacc aattcttttt tatttctcga atcttttatt
    14341 cttttaacaa actcgtcatc catcaatctt ccagataatt ctgctaaaat acgtaaatga
    14401 ttagtacctt tatcagtgtc ttttattgct aacaaaaaga cgagatcaac tggttgatca
    14461 tctaatgcgc cccattcaac agaccgcttt aacttggcta cagccactgt tgattcaatt
    14521 actgctaggc ttttgccatg aggaatggcg atatttttcc caattcctgt ggtcatatgg
    14581 ttttctctgt caaatacgtc tttttgaaat tgtttgtggt cagacacaaa cccattagtc
    14641 tctaacaatt tcgataattt ttcgactaca ctttttttat cagatgcatt taaatctatc
    14701 actatattat ttatattgat tttttcactg atcatttata agccttcttt caatatataa
    14761 actaattttt caaacgacga actcgttttt aaatttgcta ataactcatg attcgaagcg
    14821 atttcattga atgtttcata aattttattc aattcaggtt ttactttttc attaagtgca
    14881 aaaaagaaaa cagaatgaac aagtgtccca tcccaatcaa ttccttctgg attaacgtag
    14941 acacagatta ctggcttttt tataaaatct acttcgatat gcggcatagc aaaatcattc
    15001 atcgctgttg aagatagttt ttctcttttg attgctccct ctagtatccc ttctttcgcg
    15061 taattttctt ttaccaattt tttggtaatt atatttaaaa cgttcacttg attatccttt
    15121 atttggtggg taaaaaagca aaatcgttta tctaataaat ttagaaaagc atcttctgtt
    15181 tgttcattta actgtagata ctgttttact tttctgacat ctgcccctaa catcaaagga
    15241 ctaacaacaa taacaggaaa atttgtattt tcaatcggaa tcgtgcttat aacaagttca
    15301 tcagtgactt ttctatcttg tagttcattg atgcttagta catcttctat ctcaaccata
    15361 tctgcaaaac cgttatttat tctttgctct aataattttg atgttccata tccgctacta
    15421 caaactaata tcactttcgt tttgtcaggt ttgtaacgat ccaaaagtga ttgaatatgg
    15481 agtgttacgt aagcgatttc atcttcatta aaatgtatac agaatctctc ttcgatttcc
    15541 tctaataaat ctacacttat cataaatgac cttttaaaag aatatttgat tttgtccgta
    15601 taagggttat agatatttac tcccaatttg agtcttttaa ctgctgcatt gagatgtaat
    15661 actagattat tgatcaattc ttcatcagga taaaattcat ttaatttaat tcttactaac
    15721 tcttgtagct caacacccat atctaaatag gaaagcttcg tttgatctaa gtcgtgattc
    15781 atagcatttt tttcaatcga agtaatgtga atgtcaacat aagcttgttc atctattggc
    15841 acaataattg aaaatgcttt ttcaatcatt ttgattaaaa taagtgtatt ctttgagatt
    15901 tcttcttcag aattattact acgtgataag aaattgcttg tttcaatcct ttctatcgca
    15961 atagcaaggt ggatagctaa agtttgaaat tcataatctg taaatttcaa ttgtgtctta
    16021 ttcatgtatc gattaagcaa ttcggctact tttttcaata tgtccttatt taagaaatta
    16081 ttcgcttcac caatgaaatt gatattcaag tctaagagat ctgttttact gttagatgct
    16141 gtaaagccac cccaataata gttcacaact tctgacatca accttctttt acttttttca
    16201 tcagctttga tgcgcacgcc atttcgatct gaaactaagg acatcccatg cttgtttagt
    16261 ctttttctaa catctttcat tgtgctttct accgtcgacc tgctcacgta taattcgtca
    16321 gcaagatttt gaatctttag agaatcatta gtattcaata atttagcaaa cacataagtt
    16381 agacggtcct ccggagagtc tgcgatattg attttatgac tggataataa atttcgaagt
    16441 gtttcttttt ttccaataag cgaaacgcct tttttaggtt gaaccaacat ttgtacatct
    16501 ggcatttttt ggattatttc tttcaaaatt aaaatatctt tgctaattgt tttcgtagat
    16561 acatataatt gttctgataa ttctttgtaa gttgtgtgct cattttcaat caaaaagtat
    16621 agtattttca tttgccgttc gctaaattcg atgcccatta tcttcacctc ctgaatacag
    16681 cttaagtgta caattattat ctctattttc atacagaaga aagttcttga ttaatgtaga
    16741 agtttct
//
LOCUS       DAZKNH010000054        16210 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000054 DAZKNH010000000
VERSION     DAZKNH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.054"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1569)
                     /locus_tag="ACG8MG_001691"
     CDS             complement(<1..1569)
                     /locus_tag="ACG8MG_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFQ0375734.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTII"
     gene            complement(1556..3244)
                     /locus_tag="ACG8MG_001692"
     CDS             complement(1556..3244)
                     /locus_tag="ACG8MG_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375735.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            3390..5027
                     /locus_tag="ACG8MG_001693"
     CDS             3390..5027
                     /locus_tag="ACG8MG_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375736.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(5172..5912)
                     /gene="truA"
                     /locus_tag="ACG8MG_001694"
     CDS             complement(5172..5912)
                     /gene="truA"
                     /locus_tag="ACG8MG_001694"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0375737.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(6103..6738)
                     /locus_tag="ACG8MG_001695"
     CDS             complement(6103..6738)
                     /locus_tag="ACG8MG_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFQ0375738.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(6885..8105)
                     /gene="sstT"
                     /locus_tag="ACG8MG_001696"
     CDS             complement(6885..8105)
                     /gene="sstT"
                     /locus_tag="ACG8MG_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFQ0375739.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            8324..9322
                     /locus_tag="ACG8MG_001697"
     CDS             8324..9322
                     /locus_tag="ACG8MG_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375740.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            9518..11353
                     /locus_tag="ACG8MG_001698"
     CDS             9518..11353
                     /locus_tag="ACG8MG_001698"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0375741.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(11413..12129)
                     /locus_tag="ACG8MG_001699"
     CDS             complement(11413..12129)
                     /locus_tag="ACG8MG_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFQ0375742.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(12356..12898)
                     /locus_tag="ACG8MG_001700"
     CDS             complement(12356..12898)
                     /locus_tag="ACG8MG_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFQ0375743.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(12903..13130)
                     /locus_tag="ACG8MG_001701"
     CDS             complement(12903..13130)
                     /locus_tag="ACG8MG_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFQ0375744.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(13209..15089)
                     /locus_tag="ACG8MG_001702"
     CDS             complement(13209..15089)
                     /locus_tag="ACG8MG_001702"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0375745.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(15278..15892)
                     /locus_tag="ACG8MG_001703"
     CDS             complement(15278..15892)
                     /locus_tag="ACG8MG_001703"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0375746.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            16034..>16210
                     /locus_tag="ACG8MG_001704"
     CDS             16034..>16210
                     /locus_tag="ACG8MG_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0375747.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
BASE COUNT         5162 a         3065 c         2528 g         5455 t
ORIGIN      
        1 aataatagtc gttactgtat aacttgtgct tactttaata tcagcaactc ctttgactcc
       61 gcttatttgt accaatccat tagcattcac ctgctccttt acaggttgat gatttatttc
      121 tacattcgtt cctaaataag catatacagg aagtgtgatt acttggctct tctcattagt
      181 tactctatat atgatagaac taccagttgt tttataagga aggtttaccc attgactgcc
      241 aatctttgct tcgtgtttta ttatatgctc cttattctcc aatgaagcga taggagaata
      301 atcatataaa aaataatcca ttcctttagt ggtatacgcc tctactgttt ctttgtttac
      361 aacaatatgt cccggctgag aaattatttt ttgattgaaa ttttttttac tagaaacagt
      421 taaagtgatt aataacaaaa cagtcataaa taaataaaat agttctcttg tcttattgta
      481 tcttctattt ttgaaaaata tcatcgttaa tatcagagaa gagaatatta tttgcaagct
      541 taagatccgc caaggaaatt gtaacaattt ggctggcgta ttttgcagaa gataccatgg
      601 caccaaacta gttgtagcta gccaagtcaa acacgtacca taataaatct ttttttctat
      661 gtttgttaat tttttaaata aaatcagggg aatacataaa gataacagac caactagtcc
      721 cattgagtaa gtagttaagt cgttgttcat agcttcagtc agactatcga acacattaga
      781 tgcatgagca tataaatttg tcacgctagg tttgttgatt ttttggtaga gcatttgttc
      841 aaacatcgga taccaaaaag cactcgtcag aaccaatgtc attcctgccg cactaaataa
      901 ttttttaaat atagcaataa aatctgcatt cttgaaacct ttagaaacca aacgaataaa
      961 aataaaaatg ataattaatg ctgtactcat taatgttgat agtacatggc tgtacactag
     1021 caaactcata ctcaatgcta gctttacaga cggatacttt tcttcttttc cagagatgat
     1081 ttgataagca taaaataata cagggataac aagtgttatt gcgatagctt cggcaattgc
     1141 ggaacgatag tagatgtcta ctgtccgata agcagagaaa atatagagac aactaaataa
     1201 aaatgccgct tttttttgtt ttaaaaaccg ctcgccataa tgataagcta cgagtgttgt
     1261 acacaagttc aataaccaaa cgtaaataat atagcttaca atcaaatttt ttgtcatcca
     1321 atataacatt acagctggaa aaaaagtcag aaagggataa aaataattta caccatatcc
     1381 agtataattg aatgtctcat aattaatcgg actggtaaaa atagtcgata accccttgat
     1441 tcgagataag tgaaaagaaa gatctccagt ggtataaata ctctgtgtat ttttgaaaaa
     1501 gatgaaagaa tatatactac taactataat taatccactc aatattacta cttttttatt
     1561 tattttcatt cttcttgttt atctcctttt tagtcacata tcgagaatct ttcaacttat
     1621 taggtgcaaa tcgtttaaaa cttgaataca ccactaaaca ataagtcaga atactgatcc
     1681 ataaagccat gagagcagtt ggagtatatt ttgttgtaat ttctatctta ttttctccag
     1741 catttacatc cacattcact aagccatgtt ttttataaga gtcatttacg actttaccgt
     1801 ttacttttac ctgtgtacct aaataagcat atactggtac tgtcactttt tgctttttgt
     1861 tgcttgccac tgtgtaagta atgttattat ctgcagcttt atacttccct ttttcccacg
     1921 attctcctac ttttacttca tgtttttcta agtgaggcct ttcttttatc gcttctgttg
     1981 gggcgtaatc gtataatcct cccatatctg atgttgtata acgtgggagt gtttcttcat
     2041 tgataacaat atgaccgtgt tttgattgga taacttttgc gtaattttct tttgctgcta
     2101 tagacgtgac gagtagaaac accacagtag cgccaatgat taaccatgaa tattttttat
     2161 tcatagtctt tttcataaaa acaagcgtta tgatcattga agaaaaaaag atttggatac
     2221 ctaagatacg ccaaggaaac tggagtaagc ttatcggtgt tttttgaaac aaaaaccatg
     2281 gaaataaatt agttacgcat acccatgtag aaactacgcc aacatagatg attttttctt
     2341 ttttctccat ttctttcagt atgaataaag gaaaaatcaa cgcaaccaat cctaataacc
     2401 ccatagtgta tgttgtaata tcgttattta acgcttgaat gatactatct gaaatagaaa
     2461 gtgcttctag ctgcaaatct gttttgaagg gactgtgtat ttcttgataa ttcatctgct
     2521 gcaacatagg ataccaaaaa tagctagtta atgcacaagt caaaaaaaca gcctttacta
     2581 aattcacgac cacaaatttt atatcgtttt ttatttgctt gcttaaaaac aaacgaacta
     2641 taatcataca agcaatcaat gcactacaca atagtgtagt taagacatga gtatacacaa
     2701 gtagacttat actgacagat aaagcgatga cttttttttc tctattgaat atcaatccat
     2761 aagcataata taaaacagct gggataatcg taagagcgat tgcttctgca attgcggatc
     2821 gataatagat atcaactgtt cgataagttg caaaagtata taaacaacta aataaaaaag
     2881 cagcatctat ctttttaaaa aacttttctc cataataaaa actaatcaga atcgtacaaa
     2941 tatttaataa ccaaatatat agaacataac ttaatattaa gttatttgaa attccataaa
     3001 aaacgactgc aggaattatt gtaagaaaag gataaaaata gttcagtcca tctccataat
     3061 tgttaaatgt tgtatagttg attggccctt caaaaataga agataaccct ttgattcgtg
     3121 aaagatgaaa agataaatca cctgttgtat aaatttcttg agcatttctc ataaaattta
     3181 ctgaatataa agtactaagt aagataagaa ctactaaaag aaatgctttt ttacttattt
     3241 tcatagattt gttgttctcc ttttttaatt ctattttcag tatactagaa ttgtcttttt
     3301 ttaataagtt ttgcgttaaa tttttcatag cactagtatg atatactaga aaagaatcaa
     3361 tttttattac ttttaaatag gagaaaaaaa tgaaaaaaaa caaaaaccaa ataatagata
     3421 tcatttttat gttatttcta agttttgctt acgtcatccc tctattcatg tctaagggaa
     3481 tctatcatag tgtcaatcag gatacttact ttcatttatc tcgaatcata ggactagata
     3541 atgtatggag ttctccagta aacttcaata acttcgatca tcatggaaca atgatgaata
     3601 ttttttatcc atggcttact ctttatccag cttttttatt ttataaaatg atgggaaatc
     3661 ttgttttaag ttataatatc tactattttt ttatcacttt tttaacaatg gtcgtttctt
     3721 atttttctat gaaacaaata aaaaacaatc gttatatttc gctgttattt tcgattattt
     3781 atacattttc cgcctatcgt gccattgaca tattccatcg tgcttcattg ggagaagctg
     3841 tcgctttaac atttttgcct ttgatactaa tggggtgtta cgaaatttat attagagatt
     3901 accaaaaatg gtactggttg tctattggca tgacgctagt agtgtatact catttacttt
     3961 ctgtcgctat ggtttctgta tttattggag gaacgttgtt tctttctttt tatttttggg
     4021 accaaaaaat cgctagactc ttaagtcttt taaaagctac cgtcctcacc ttttttttaa
     4081 gtgcgggatt cttaattcct tttattcaac aatctagagc acaagaattg aaagtcccac
     4141 tgggaaaaga attaagtggt atggctccta gtgatatgct gacacatatt ttaaataata
     4201 actataacaa ctatactatt ggattattct tatttctcgg attgattggt gcttttattt
     4261 ttataaaaaa attaactgct gatgatttat ttattttctt tcttggtgta tttgttcttt
     4321 tttgttcaac caatttattt ccttggcaac tttttaacca tacaccagtt aagagtctac
     4381 aatttgtctg gcggttaaat ggtttttctt ctttatttat agcctatacg atgagtatag
     4441 tgatctatta tattttttct actaaaaata attcatggaa aataatggtc tttacgtttc
     4501 ttgcactaat attgcatctc tcaggagtaa gcaattctat ccatgctaac aaagattcat
     4561 taacacttat agcacctgaa gacgctgttg ctatagctga aaattacaat catactgatt
     4621 atgcaaacaa agagtcaatc tatcatcctg atatgattaa taatgatcaa tatttactag
     4681 gtaaccagga aataaatcca acaactcatt ttatgagtaa caaacttaca attgaattag
     4741 ataattcgaa taataaaaat actgtcttga cgacacctat ttatcgttat aaagggcaag
     4801 tggcttctat taatggcaaa ctagtacaaa caaaattatc taagtttggc acaactgaat
     4861 taactattcc tccaggtata aataaagtcg ttattactta tcagtacaca aaactagcaa
     4921 ttgcttcaag atatttatct atcgttactt tgattttatt tttactttat cgatttactt
     4981 tttctaaaca aaaacaaccg agaagagaag tacaacattc tcactagatt tttatttaag
     5041 aaagactttt tagctgtaca cataaacagg ctaaaaagtc tttttttata tttctttaat
     5101 ttgcttcata cattatttaa catcgatatt tatactgtga aacatgcgtt tcatccatta
     5161 tcaaagtatt tttatcgata ataaacttca tccaaaaaca gaccttgtgc agggacggtt
     5221 tctcctgcat gttgccgcac tttgctttca aatatttcat cgattgtttc tatcggcatg
     5281 gcacctgtgc cgatttctag taatgtcccc atgataatcc gcaccatttt atataagaat
     5341 ccgtctccga caaaagtaaa atgaagcatt tctccctttc gttcgataga taattcatca
     5401 atcgtacgca ctgtggattt ttttgatttt ttcaatgcag aaaaaccaat gaaatcatgt
     5461 gtacccacta atttttcgca agctgcattc agcttgtcca tatccaactc ttgagggaaa
     5521 taaaagctgt ggtttcgctc aaatgctgaa ggaatcacgt tgttccaaac ataatagctg
     5581 tattttttgc ccaccgcatt atatcttgaa tggaagcgct caggcatttc ttcgattctt
     5641 ttgatgacaa tatcccgcgg aaggtaacga ttcataaagt cgatcatttc tgccagtcca
     5701 atttctgtgt ttgttttaaa atttgctact tgtcctcgcg catgggtgcc agcatctgtt
     5761 cttcctgatc caataatttc tatctcttca tttgtcatct gatgcaatac tttttcgatt
     5821 tttccttgaa tggttttatc gtcatctcca agtctttgcc atcctgaata tctttttccg
     5881 tcatattcga ttgtcaactt gatgtttctc atattttagt ggctcctctg tcttccattt
     5941 ggtcttctct ctaccttttt aagattgtag cacaaaatat agctccttgc ttctctaaga
     6001 cagaagctat acaagaaaac tgtggaaata tatatttcag aaaaacaaaa aacagccacc
     6061 ggatgaagta tccgctgact gttcaatttg acggctcaag ttttactgtt tgagatgcaa
     6121 gatactcaac caggcaatgg gcacataatt aaagaaaaaa ctgattcttt ggcaaaaacc
     6181 tattttattc agtaatggcc aagaatttat agaaggaatt cttgccagtc cataaatcgc
     6241 cgcaaaaagc aaactgatca cagacagtcc aaggtataag tggcttacgt ccatttgccc
     6301 attttttcga tagatcaaga aaagaagaac tggaaacaat aaaaaaccac tgtaacctaa
     6361 acctgatccg atattatgga cccatgtcga gacattccac gaagattcat gggtattgat
     6421 actgaacaaa cctgtaaaga tacaatcacc tataccatag aacgcgatgg ctgctgtaac
     6481 tagggtagct aacacacgag atgtactaac aaatgcagca tacaatgcag gcaatgacag
     6541 gatgaaaaag atccctgaaa ccacagacca aataagaaac gcttttctta caggactgga
     6601 aacctctcca aatacactga tcacgctctt catttgatct aattcaggat aatacaatcc
     6661 taatacgtac ggagtcaaaa aatcactcag cacgcctaat aataaaaaga aaaaaccaaa
     6721 tttacgtaaa aaatccatga tacgcctcca ataacaatta cctatgcttt taattgttat
     6781 tataaacgac tttatcttga tttaacagaa acctctattt acacgaacat ctatataact
     6841 cattaaaaac tcgaatcatt ttaaaaatac taaatatcta acgttcaacg ttcacttaat
     6901 ccatctaaac aatctttcaa tttgattttt tccccacttc ttttacgcgc gcctagctct
     6961 cctattgcag taaacaacag atcacttgac gaatttaagg cagtttccat tgaatcctgg
     7021 ataacaccga taataaatcc aataccgacg atttgcatcg cgacatcatt agagatacca
     7081 aacaaactac atgctaatgg aatcaataac agtgaaccgc ccgcaacacc tgaagctcca
     7141 caagcagcta aagcagacaa aatacataaa aggactttca aaacaaaagg tgcttcaatc
     7201 cctaaagaat gtacagcagt caacgtcatc aaggaaatgg taatcgcggc accgcccata
     7261 ttgatcgtag cacctaacgg gatcgatatg gaataagact cttttgtcaa tttcaatctt
     7321 tcagccaaca tcatgtttat cgggatattt gcggcagaac ttcgggtaaa gaaagcggga
     7381 agtgcgcttt ctttcaaaca aaagaaaatc aatggatagg gattttcttt aataaacaaa
     7441 aacgtgagga atgggtacac aactaatgcg acaaacagca tcgtaccgat caaaacaagt
     7501 aaaagctgca gatactctgc caatccagcg attccagtcg tagcaattga ttgataaacc
     7561 aatcctaaaa tccctatcgg agcaaatgca ataatcactt gtacgacttg tgacaacgct
     7621 atagatatct gatccaccaa ctcttttgtc gattctgaac gtgctcgaaa agctagtccc
     7681 aacaatacag cccagaataa gacgccaagg taattcgcat caataagaga ttgaacagga
     7741 ttttgtgtga cattcgtcaa catagtagat aaaacacttt ccaaactttg cggtgctttt
     7801 tcagatactg tttcttgtaa agtaatagga acagtaaaaa ttgaacttac aataactgct
     7861 acaagtgcag aaagcaatgt tcccactaaa tataagatca aaataggctt cacgaatgtc
     7921 ttattcccaa ttttatgctt ggcaattgac gccatcgtca agaaaaaaac cagaataggt
     7981 gcgattgcct tcaagcaact gacaaacaac gttcccaata ttgaaataaa tgtccatgtg
     8041 gggaaaaaga taccaaacaa tattccgact aaaataccaa caacaatacg ttgaactaac
     8101 gacatatcca aaaacttctc taacaaatga ccaccctttc acaattttaa caatcattgt
     8161 atcattttat gcaaagaaac taaaggtata attttctaaa aattctaaca atacaaacaa
     8221 ccatttcttg ctaattttgt actttttctg tctacacgtt gttttttgag atattctaag
     8281 ttaggatgtt attaatgggc aacttatcag gaaacgtttt cttatgtttt tgtccaattt
     8341 ttttgtgagg tgtttattct tgaaaaaaat aaagaaggag agttatggct tcctgacaat
     8401 ctttgtcgct tgtataatga tggttttaca gcgtatattg atttcagcag tcactattcc
     8461 tacatatgca acaaaccctg gtcgattcta catctataca tttttacaaa cttccgttgt
     8521 tattggtgca aatttgatcc cactttacat gggttaccaa tatcaaaaaa tcgcccgttt
     8581 aaaagtttta cattatttaa gtcgttttgc ctttatattc ctaatagcca cactagtttg
     8641 caatatcttt ttttacgtac aagctggcag cttgaatatt cgtgattatt ggcttatcct
     8701 aacacctatc agtcagaatt actttactta tgctgtttct tgtactttgc ttttttgtag
     8761 tattccttat acagttggat ttttaaataa attatcgaaa acaacaatta aatatctact
     8821 attattttta acaattggat tagttggctg ctctactcta tttaataaag acccgtttgc
     8881 attacagaat ggacagagta ttttatggat tttttattta tttttaatgg gttatggttt
     8941 gaaagaatgg aactggaaag aaaaggtccg ctataagctt cctcaagtat ttatcagctt
     9001 gattgtttta ttcggtttga ttattttgat gacacaaatt tcactcatca tcagaggaaa
     9061 tacagataca gcgctgaggt tttctaaacc atattctctc ttcgcaatgt attatacttt
     9121 ctcttcgttt ttatttttag aaatgctatc tgataaatta ggtataaaag gaagaggaaa
     9181 ttttcttgct gtatttttga ttgtcactca agttatatgt aatttccctt tagtttctta
     9241 tcgcgtatct acatttatga aacgtgaatt tccaaactca gggaaggcat ggctgataaa
     9301 tatcgggcag ttttttattt gatatttatt agcagtgtcc ctcctagtta tctttttatt
     9361 ttatatacaa aaaatccgtt tcattcatcg tttcatcgaa tacttcgctt ttgattcgat
     9421 tgaacaatta actcagcgtt ggaaaaaaag aattcattgg ctttttgttc ataaaagtta
     9481 ttttttagtt gcagcatttt tttactgctt taccttcgtg cagattttcg ttttagaacc
     9541 aaaagaaggt tggaaagaaa ctatccaggt tgccttaaaa atatttactc agcgacaagc
     9601 acccatgatc ctgacaatca tcattattat gggatttttc ttcttactat tgttgatttc
     9661 caatcgtttt tggtacgcct tgactgctac cttgatcatt aacttactgc tcactatttc
     9721 gacagtaatc aaaatggaaa tgcgggaaga gcctgtcttt ccttcagatt taaaaatgct
     9781 gaccggattg tcagaactat tatcgatggt tagcccagtc ttattaatcg tgggcggatt
     9841 gatccttctc ctattattga ttactagtat tattgtacaa cgtagactac aaaaacagta
     9901 ttccttgaag attcattgga aacgtcgatt tatcagtatc gctgtattat taggatgttt
     9961 ttcgggtgtc tttttcatta atcacaaaaa ttcgccttct ttcttgctgt ttaatttatt
    10021 taaagtaaac aagactttct ttaaccagca aggcgccgta aaagataacg gaccagtcat
    10081 tcaattccta aacaatatcg acattaaaat catggaagaa cctgctggct attcgaaaga
    10141 aaaaattaat ggaataatga aaaagtacaa cacaaaggca gatgaaatca accaaacaag
    10201 aagtgactgg ttaaacaatc agacgatcgt tctcaattta agtgaaagct tcagtgatcc
    10261 tgcacgtgtc cctaatctga cagtacctac agaccctatt ccttacatca aaagcattat
    10321 aacagaagga acaggaggat tgatgttatc cgtcggttat ggtggcggaa cagctaacat
    10381 ggaatgggaa ggactgacag gcttgagtat cagtaacctg tctgcttcgt tagttacacc
    10441 ttatacccag ctagtggagc gtcagaaaat aagtccaaat attaccgatc tattcgatga
    10501 aaaaatagcc atccatcctt ttacagctgc tctttatcgc agaaaacaag tgtttgataa
    10561 atttggtttt gacaggttct attatttaga cagtccggat aaattaactt atacggataa
    10621 aatagaacaa agcccacgca tatcggatga ttcagcatac aaagaaacct tgaaagcttt
    10681 acaaagcaat acggaaacgt cacaattcat ccaattatcc actatgcaaa accatatgcc
    10741 ttatgatgat tactatagtg aattgaacta cactgctgaa ggaactgctg tcattcctaa
    10801 tagaagaaaa gaattacaaa cttatatgca aggccttcac tataccgata cagcagtaaa
    10861 aaaattcatc caagaaatcg acaaaatcga taaaccgatt acctttgttt tttatggtga
    10921 ccatttaccc gcaatctata gtgggaatga ttcgaaaaaa tatggattag aacagcacca
    10981 gacagattat ttcatttata acaacaagta tagtcgtgaa caagcagcta cgttaaataa
    11041 agaagttgtt gctcctaata actttggtgc tatggctctt gagcatgcga atatcaaagt
    11101 aactccttac tatgcgttgt tgacagaagt atctaatcat ctacctgcat taacaattga
    11161 tcctacagaa agcttgtcta atcgttttaa tggaaaacag gtctttgtta cagaggatag
    11221 tcaaattatc caagaaaaaa atttaactga gaaacaaaaa gaaattctag aagattatcg
    11281 tctgatacaa tatgatcttg ttgcaggtaa ccagtacagt gcaacatggg cgacacaaaa
    11341 aataaaagac taacaaataa actgagcaag gggttgtgat aaaagacatg tcacaacccc
    11401 ttgtttatct cattaatttg cttgtccata aacgatttga ttaaagccat ttttcaattc
    11461 attattcgct gtctcgttga agtaaagata attatcggga ttcccctgat tttgttgtgt
    11521 caccaatact tttggctgat tttgctgaaa cccgaataga tagacgtgct ttttcagata
    11581 tggttctgtc tctgcatcgg aatagacggc tgtgatttga tagtctgctt gacctgtccc
    11641 actttctgac cattctgcag aaacaggcac tccaccaata gccatcttcc actccccgtt
    11701 caaaatagct tgagggactt taagaccata taagtccaca ctcatttgat tcgtataact
    11761 tttgtattgt tgtcccattg tttggcccca tttggtcata aatgcctcta attgtgcgga
    11821 tttgtctgca ttccacagtg tttgttcaac ttgttcgctg ctttctgaag aggaagatga
    11881 agcctctaca gaagaacttg aagtagatgt ttgagttgtt tcagtcgttt tggaagactc
    11941 tgacgaagta gaggtatctg ctgtttctgt acttgctgca gttgacttgc tctctgtgct
    12001 agttgttgta gtagttgcaa ctgttgcttt cgctgtttct tttgttgatt cttttttcga
    12061 ttcattcccg ttggtacaac cagctaacaa aaaagctaga ccaattgtta ctaaaatcct
    12121 ttttttcaat taaaaaactc ctttcgatta ctcccacttc tttattctat caaataagca
    12181 atgtgatttt attgattttt cgtattttct catcaatctt tcaaatcttc taaaaactga
    12241 gaagcacccg ctgatgttgt ctgtaataca tcagcgggtg cttcttttat tttaatcttc
    12301 gtgattttcc aatttacctc acgctttata tttgtatttg aagaaacata gacttttagt
    12361 catcatcgtc ttcctcatcc aaatcttgtc ggacagtttt tccttggagt ctttgtactt
    12421 ggtagatgat ttctttattg aaacttaaaa gttcaacaag caattgttgc aagccatatt
    12481 tttcttcttt ttcagctaat ttgattgccc gtgtgataaa gacattttgg aaatcaaaat
    12541 cttttgcaaa gttttccaac attgttgagg ctggtaaata tttgttttga ccatgttcgt
    12601 aaatttttcc ataacgcatg acttcttcta aagtagacgg aacgatttca ttttcaatca
    12661 ctaactcatg agagatttta ttgaaccatc tcgcttcttg atcagcatat tctgaaaaaa
    12721 gggtacttaa ttcttgcgaa gatgtaccca aaacatacaa ttgtgcttgg atcaatttca
    12781 tttcttctaa tttaaaattt gctaatacat gccccaccat ggcgccagca gttggtttat
    12841 gatggtcgat atctgcttgt tttgtttctt gaacaaattt ttcttccact atattcattg
    12901 atttacgcct cttttacttt tgctgattgg acttcataac ctaatttttc cacaactgca
    12961 gctaattgtt cagggtggac ttcatctgga tcaaagtttg ctttgatttt gctcgcatta
    13021 aacaaaactt tcgtattgct taccccaggc tgttgagaaa ccgcatgttc gattttttgc
    13081 atacagcttg gacaagctag tgtatctaat tgcataataa tttttttcat cgtagttctt
    13141 ccttcctttt tcttgatagt cttattctag attacagctt ccataaaaaa attgattctt
    13201 atcaagtttt acgatttttc ttctttgctt tcgtgtccaa aatacaataa acgcatcgcg
    13261 ttaaagataa cgatcaagat actagcttcg tgaacgaaca tcccactagc catgtaaata
    13321 aacccggcaa ataagccgtt gagtaaaaat aagacgacac caatggcaat cactacattt
    13381 tcacgggtat ttaaaacagt tttcttagcc aagcggtagg catgaaccaa cgattcaaaa
    13441 ttagattgca tcaagacaac gtcactcgtt tcgatagcaa catccgttcc tgatcccata
    13501 gcaatcccga tatcagccgc agccaaagag ggagaatcat tgattccatc tccaataaaa
    13561 gcaacgcgca atccttgttc ttggtatttt ttcacaaatt gaaccttttc gtctggtaat
    13621 aattcagcat ggacttcatc aatgcctaac atatcagcta cataatcagc agtcatctga
    13681 ttatcacctg tcagcatcac taactgtttt gctcctgctt gtttcaactt agctaattgt
    13741 tcagcgactt ctggtcgaat cacatctgac acacctatga tttgagtgat ttttttgtcg
    13801 attgctacaa taatgaccga gctgcctgtt tgttgtaatt gatgtaaatc ttgtacttgc
    13861 tttggtgtga gagaaagttt gtgagcggca atcaatttgg tgttgcccgc acaaactgta
    13921 tgaccatcaa tctcagctgt gattccttgt cccttgattg tctgattatc tttgactgct
    13981 aattgttgaa aatcaatgtc tttctcctga gcataatgga tcacggcgcg ccccagagga
    14041 tgatcggata acgtttctaa tcgagccact aaagctaagc tgtccccttc atcctctgta
    14101 tagttcttga tagtggatac agcagtagtt cctttggtca atgtaccagt tttatcaaag
    14161 acaaaagtat cgattttgga aaaagtatcc attgcttcac cgcctttgac taagatgccc
    14221 cgtttcgctc cattaccgat tcctgctaca ttcgaaacag gcgcaccaat gactaaagcg
    14281 ccaggacaac caagaaccaa aacagtgatt gctaaacgaa aatcacgact aatcagacca
    14341 acaaccaacg caataagtaa aacggttgga gtataatact gagaaaatcg atcaatgaat
    14401 ttttccgcat gggatttggt atcttgtgct tcttctacta attcgataat tttggcaaag
    14461 gtcgtatcat ctccgacctt agttgcttca atcgtcacgt agccatctgc taaaatcgtt
    14521 ccagagaaga cttcatctcc actgattttg acgacttgtc gtgattcacc agtcactgcg
    14581 gcttcatccg catacccttt cccgtcaatg actcgtccgt ccacaggaat agctccacca
    14641 gtttttacta agacgatatc gccttcttcc acgtcatcga tttccacttc ttcagtcgtt
    14701 ccatcttctt ggacaacact agcagtagat ggggccatct cggtcaaagc cttgattgac
    14761 tcccgcgttt tggtcaatgt tttttgttcc agatagctac caaacaaaaa caagaatgta
    14821 acgatagctg actccttgta ttctccgatc ataaaggcac caagcacagc aatagtaacg
    14881 agtaaatcaa ttgagataac tttgactttc actgcctgat acgcatggat cataataggt
    14941 atgaccgcaa taatagaagc tatggctaga gcaatgttat agaacaagac aaaaccagta
    15001 aattttccga taaaaccaaa aataatcaac acagctgata aggcggtgat atgatttcga
    15061 tgctcttgga tccacttttg taatttcatc tttctccctc ttttcctttc attcttacga
    15121 gactaagtat agatacttat ccatcataaa aaattgacca ctatcaagtt tagtcaaaaa
    15181 ggcggttttc ttttggtaga atacactaca aaaaatagtt agcaagaaga ctcactaaaa
    15241 gtgatcttgc caactattgt cgtttctatc gaaattttca ttgaatgatt ttcctgtaca
    15301 gcatacatcg gagatctatt tctttatcta agatttttat cttgctaatt ttttccttta
    15361 gtctttcaaa tccacgtcgt tcatagaatt gatacgtaca gccactatct gtaaaaagga
    15421 atatttcttt tcctttctct cttttttcaa attctgttag caacttggta ccgatgcctt
    15481 ttgttttcaa atcaggattt gcagctaaaa aaatgatctc tccatctggc gaatgcttct
    15541 ttaaatatgc tgcaagcatt tgttcatttg ctgtctcgta agctccttta ctttctttag
    15601 caaatatatt ttgtaaaaaa tcgaatatct taacatatat ttgctgtcca aaagaacgat
    15661 gtttcttttt ttctcctttt atctccgcca gcaaaacacc tgccaactcg tcctctgcat
    15721 acaaagcaag aatttgagtt gctcgattca actccatata caaaaaatat cttccgtaaa
    15781 gattcagcag aagttcattt tccatatatc tctcaaagtt cattccttca acagcaaaat
    15841 agatagcttt tttgtaatct ttcttttcta agggtttaaa agttagtttc atcatttcgc
    15901 ctcctcaact tcaatgattg tgttatactt acattataat agacacagtg tctactatct
    15961 ataatcaatc atctataagt ttgtcgatta agcgacagct ttgctttgat atgtcggata
    16021 aggagatagg taaatgaaaa agacagatag acgcgtcaaa aaaacagaaa aagctttggc
    16081 tgaaacacta tccacattat tagtaaataa aaagattcaa gcaatcacta tacgcgaact
    16141 aacagaaacg gcagatgtac atcgttcgac tttttataca cattataagg atatctatga
    16201 tctatatgat
//
LOCUS       DAZKNH010000055        16183 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000055 DAZKNH010000000
VERSION     DAZKNH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.055"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..440
                     /gene="scm"
                     /locus_tag="ACG8MG_001705"
     CDS             <1..440
                     /gene="scm"
                     /locus_tag="ACG8MG_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0375748.1"
                     /translation="STTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSD
                     STDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDN
                     GNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(595..1581)
                     /locus_tag="ACG8MG_001706"
     CDS             complement(595..1581)
                     /locus_tag="ACG8MG_001706"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0375749.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1604..2170)
                     /locus_tag="ACG8MG_001707"
     CDS             complement(1604..2170)
                     /locus_tag="ACG8MG_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFQ0375750.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2184..2600)
                     /locus_tag="ACG8MG_001708"
     CDS             complement(2184..2600)
                     /locus_tag="ACG8MG_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFQ0375751.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3057..4991)
                     /locus_tag="ACG8MG_001709"
     CDS             complement(3057..4991)
                     /locus_tag="ACG8MG_001709"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0375752.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5293..6228
                     /gene="pplA"
                     /locus_tag="ACG8MG_001710"
     CDS             5293..6228
                     /gene="pplA"
                     /locus_tag="ACG8MG_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFQ0375753.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6318..7421
                     /locus_tag="ACG8MG_001711"
     CDS             6318..7421
                     /locus_tag="ACG8MG_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0375754.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7434..8387
                     /gene="menA"
                     /locus_tag="ACG8MG_001712"
     CDS             7434..8387
                     /gene="menA"
                     /locus_tag="ACG8MG_001712"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFQ0375755.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9201..10547)
                     /gene="gor"
                     /locus_tag="ACG8MG_001713"
     CDS             complement(9201..10547)
                     /gene="gor"
                     /locus_tag="ACG8MG_001713"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFQ0375756.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10681..11934)
                     /locus_tag="ACG8MG_001714"
     CDS             complement(10681..11934)
                     /locus_tag="ACG8MG_001714"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFQ0375757.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11940..12863)
                     /locus_tag="ACG8MG_001715"
     CDS             complement(11940..12863)
                     /locus_tag="ACG8MG_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFQ0375758.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13084..15576)
                     /locus_tag="ACG8MG_001716"
     CDS             complement(13084..15576)
                     /locus_tag="ACG8MG_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFQ0375759.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15583..16050)
                     /locus_tag="ACG8MG_001717"
     CDS             complement(15583..16050)
                     /locus_tag="ACG8MG_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFQ0375760.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4753 a         3384 c         2877 g         5169 t
ORIGIN      
        1 catctaccac aacaagtgat agcacggaca cgtcagcttc aagtgacagt acagatacta
       61 cctctaccac aagtgatagc tcagattcat ctaccactac aagtgatagc acggacacgt
      121 cagcttcaag tgacagtaca gatacatcct ctaccacaag tgatagctca gattcatcaa
      181 cttcaagcga tagtacagac acgtcgtcta ccacaagcga tacacgtcat gcaagtgtct
      241 ccttctcaaa aacaagttcg gataacagga cgaactttaa cttaactaat aaaacaagca
      301 atgacaatgg aaacaaatca aaaagagcac ttcctaaaac tggaagtcaa tcgaataatt
      361 ggataacact cgcaggcgtt atattacttg taatcggttt aagaatcact tttatgagtt
      421 ataagaaatc gaaaaggtaa aaaaatattc aagataaaaa agagcaacgt cgctaacgga
      481 cgttgctctt tatttttgct ttttatcata aatacaagaa ctacaattca atttgtcaaa
      541 aaacacgtag atttccttct gagaaattta tttacgtatc gaacctttcc ctattcagtc
      601 tttgcgatcc aaaatcagtt gggtaaggga aaagagattc tcttttgttc gaagagaggt
      661 ggcaggtaat ttctttattt ccttcaaagc cttttctgtg taagaggccg ctaacttcct
      721 agcttcttcc acgcctcctg tatgttcaac gattttgcgt acggcgtcga catcttcttc
      781 tgtcattgcc gctttcttct ctaaataagg tagtagcttt ggtttgtttt tttgcaaaga
      841 gtagatcaac ggtaaagaat agaccccttg acgcatgtct tctagtacag gtttgccgat
      901 ttcctggctc tcttttgtat aatctaaaat atcatcgata atctgaaagg ccatcccgat
      961 agaaagtccg atatctcctg ctttttttgc gaagcgctcg cttgttccac tttcaaaagc
     1021 gccaacagaa caacttaatt ggaacagttc agctgtctta cctgaaatat tttccaaata
     1081 ttcttttaca gaaagttcat aattatagcg atcatccatc tgccctaatt cgccattcaa
     1141 tactttttcc atactgcgag aattcaattg gagacttttc aaggaagtgg agtaatcaga
     1201 caatagttta aaacagcaga caaataaata gtctcctgca tagacagaaa cgacattacc
     1261 aaactgacta cggatattag gctgaccacg tcgcagatcc gcttcatcaa cgatatcatc
     1321 atggatcagt gtggccgtat ggagcatttc gatagaagct gccaaagcga tggctttgtc
     1381 tcgatcttgc tctggtccaa attgtgagaa caatagctga taggcaggac gcaacagctt
     1441 tccgccggca tggatcgttt ctttgattgc ttgttcgaca ggtttgtttt tcaggcggac
     1501 agtttcttcc atccgtttta gtgtttctgt caattcttta cttaaagcag gatagttttc
     1561 ccacattgga tgtagcttca taaaaaaact ccttgtgtga cgattatttt ttggtaaaca
     1621 ttgggctcca tttttgtttg gttccctgct cataatcttt ttggaatcgt tgaagtgtct
     1681 gcacatgtcg taaaagataa ttcgctgcga tcccgattgc aatccctgat aaaatcccta
     1741 aaactgaaag gactggcaag tacaacatga ctgtccatga ttgtgcaatg aaagaagcca
     1801 tcaaaagctg tccgacatta tgcatgaaac cgccggccgc actaattccg atgatactga
     1861 cacgttttgg tcctaattgc ttgaccaata acattccaaa ataactaagc aaagcaccac
     1921 tcatactata aaggaatgtg gaaatcgttc cgcctagcaa agtagtcagg aatagtcgta
     1981 gccaaagtag taagaagctg tctcgttttt tcatggtaaa aatggcaacg atcgtgatta
     2041 gattggctaa gcctagtttt gcaccaggcg caaaagaaaa aggataaggg atcatatttt
     2101 ccaataaacc aatgatcact ccttgtgcaa caagtagcga aatataaatc attttttgta
     2161 atttactcat ggtgtgtgaa ggtctaataa atcagactgt cttcgtcacc tccatctgtt
     2221 gaacggactt cgatcactaa tttatgtggc aaacagacga tggtttcgcc atttttcgtt
     2281 gcccagcctc gacgtacgca gatctggtca tcgcaatctg cttcttttat ccgtactcga
     2341 tcgccatcta tctcgatcaa gttataatct ccgtgttcat ctttgtaagt atatgtgttg
     2401 ctctcttctc cagccatcaa agggaatgtt ttgatttctg tgccatccac acgcagtacc
     2461 gcttcttttt ctgctgttgc ggtgttttgc tgaagaccaa aaatcatgac tggtaaaaaa
     2521 gaaagaacag tcaaaagcaa aataataaaa acatcccaag gtttgagcag acttttttca
     2581 gcaaattctt taaggctcat ttttgttctc ctttttccag attaactcac ccaattttag
     2641 cataaaaaca agaggttgtg acacctgtgt cttgctttga gtaataagaa cgaagaatag
     2701 aaaacagctt tccatgtttt ctaattcttt gggcttatta ccgagaagca gcaggtggaa
     2761 caccgtttat aacaagaggt tgtgacagaa gtgggcaact tcaagaaata agaaggaatt
     2821 tacgaaaatt acttttcaag tttttgtgaa atttcggctt atttttcgaa gaagctactt
     2881 ctggaacacc gtttataaca agaggttgta acagaggttg tgacacctgt gtcttgctca
     2941 tggaggctta tctcgggcat cttattcctt taattttaca taaaaaggag gctgtgacgg
     3001 tttgtctcag cccctttcta ttgaaatctt gcattcaaat aacgttcttt atctatttat
     3061 ttcttgccgt actgggaact tggtctaccg taccaccaat tacctagtgt acgttgtagc
     3121 catggaatta cccagcgatc caacccaatc gcacgtccgg aaccgttcat caatgcgaaa
     3181 gcaacaaaaa tgaaccaaat gtttacccag taaaacatgc ctgataagca gaacgtgatc
     3241 accaatacaa tcgttgtcgc actgcatagc catgtgaata atccagcaat caatgctaat
     3301 gccaaggcaa tttcaaagaa taccatgaat ttttggaaga acattgctac atcattgttt
     3361 ggcatgatca cttgcatgat ctttgtgaac cagtctggtg acttctcaat cacagccatt
     3421 ggttcttcac cgtatgcata actcaaacca aaatgtgcag cttgagtcgc tgcttctccg
     3481 gcacttgccc ctgctccgct ggcagctgtg gttgcatcac cagcagcaga agcaccactg
     3541 gcaacatctg ttacattgta ttcccatgtc caagggaaca cactgtttcc agtgaaccat
     3601 gatccttcac caaaccaagt acttggtttg aacacttcac cattcccaat gatttttttg
     3661 gaagcttcaa cgaaccaaac taaaccatag aaaatacgaa gtggcacact ccataagaca
     3721 ttgccgtaac gagaagaatg tcctcgagtc acgttgcgat catctttgat atggaaaaac
     3781 tcatgcatta aatattggaa catataataa ccagaacgaa tatcaaagaa gtatttcaaa
     3841 ttcacgatat gtttcattac gatagctaaa aatccgctta aatggatctt atcaaatagg
     3901 ttagctaccc cccatttggc accaatagaa accataaatc cttgatagtt tcctttaaat
     3961 ttatgctttt ctccgccttt gatcgaagcc acgatattcg ctgcggctgt gtgtcctgtt
     4021 tgttcagcag cttgaacgat ctgcggtgtc ggtgttcctg ggaattcttc ataaaagact
     4081 aaatcaccaa tgatatagat gtttttatct tcgtagcctt ttgcctgcat atattcattg
     4141 gcgatcaacc gctgtccgcg tgctgattct aatccaaagt cagcagcgtc agaagttcct
     4201 ttgacaccag ctgtccaaat cagtgtgtga gtcggaatgt tgcgtccgtc tttcaatttg
     4261 atatgatcag cagccacttc tacgattggt gcattcaata ataattccac gttgtgctta
     4321 cgcaaatagc gttcagcttt tgcagcatca tttcgatcca acatattcaa gatcgtcggc
     4381 attgcctcta cgaccatcag tgtgatttcg tttggatcta atttataatc tttggctagg
     4441 cgatttttcc agtcgatcag ttccccaatc atttcaatac cggtaaatcc agaaccacag
     4501 acaacgaaag tcagcatggc tttacgcagt tcatcatctg gttccaatgc agcttttgct
     4561 actgtcttct caatatgttc ccgaatcctt acagaatctt caaatgacca aagggtgaat
     4621 ccgtgtgctt tgacaccagg tgtaccaaag tcattcggct ctccgcccat tccaaggatc
     4681 agctgatcaa aaggatagga acctaatttg gtcttgacga tttttcgttc tttatcgata
     4741 ccgattacag tgtctgtgac aagttgtaca ttcttttgac gagcaaacaa atgctgcaga
     4801 tcgtattgga ttgcttctgg ttctacacgt ccgccagcta cttcatgcaa ctcggtcatc
     4861 atagtatggt acgaatgacg atcgatcaga gtaattgcta catcttcttt tttcagtttc
     4921 tttgccaagt attttgtagc tgaaacaccg gcatatcctg ctcctacaac gacaatattt
     4981 tgctttgcca ttgatcatcc gctccttata gactatctat ataatgaata tatgataatt
     5041 cttttagttt gatacttatt acacaatctc catatattat atagatgttc tgttcttttg
     5101 tctaactcct tttggatttt tttcaaattt taaaataaag cgttttttca cgaaatttgc
     5161 tgaaagaaaa cgtttgaaat attacatggg ttatttatca aaaatagttg acttataggc
     5221 taaaaagatt agaatgtata atgaatcaca aagaaaaatt gtggactaga aatggaatgg
     5281 tggggtatta aaatgaagaa aaaacgtttt gtaactggat ttgccgtatt agcattttca
     5341 gctctagtgt taggcgcttg cggcgcagat gacaacggaa gctcaaactc ttcttctgaa
     5401 tcatctacag cacaatcttc gactgcaaaa agcacaacag aatcatctgc aaaagttgta
     5461 gctggcggag atttgcaaga cggcacatat aaattagaag aaaaaaatta ttcaaatggt
     5521 taccgtgcag tctttgaaat ggttgtaaaa gacggcaaaa tcactgaatc aaaatatgat
     5581 aacgtcaatg aaaacggcga atcaaaaaca aaagatgcag aatacaacaa aaacatggaa
     5641 gcaaaatctg gcacaaaccc agaaaaatac attcctgagt tgaatgacca attcttaaaa
     5701 gcacaaagcg caagcggtgt ggaagtcgta actggagcta ctcactcatc tgaaagcttc
     5761 caaaactacg cacaacaatt gatccaagct gctcaagctg gtaacacaga tacaatcgaa
     5821 atcgacaatg gtgctgattt gaaagacggc acttacaagc ttgaagaaaa gaactactct
     5881 aacggctacc gtgtgcaatt cgaaatgaca gttgctggcg gcaaagtaac tgaatcaaac
     5941 tttgattata tcgacaaaga cggtaaatca aaacaagatg atacagaata taatgaaaat
     6001 atgaaagcaa aaagcggtac agaaccaaaa acttacatcc ctactctgaa cgacgaattc
     6061 gtcaaagcaa tgggcgaaga agatggctca cctgctaatg tagaagttgt tacaggtgct
     6121 acacacagct cacattcatt catcatgtac gcacaacaat tagtgaatgc agctgaaaaa
     6181 ggcgacactc aaacaatcga agtagataat atcgtaacag aaaaataatc acataatcat
     6241 actaataaaa gttggagggt gggacgatag ccagattagg cagtcgtctc acctttcaaa
     6301 atgaagagag gaaaaagatg cggaaaaaac tactattttc cttaatcaca ctgggcatct
     6361 cttttatggt gctaactggc tgcagcaact cagcaaataa aacaaccact gaaagttcag
     6421 cagctaccaa gatattgaaa gatccatatt ctgatgaaca gttcttgtta gggacttacg
     6481 ttcgtatccg tgtgtatgat gaaggcaaag aatcagcatt gaaacctgcc ttcgatcgag
     6541 taaaagaact tggtgataaa atcacgatca atcaaaaagg ttctgagatc gatgaagtga
     6601 atgagcaagc tgggatcaaa ccagtaaaag tttctgatga cgtctacacg ttagtcaagc
     6661 gggcctacga atatagccaa gattctcaag gcggattcga tatggcgatt ggtgcaatca
     6721 cacaattatg gcgaatcggt tttgatgacg ccagaaaacc ttcccaagaa gaaatcgatc
     6781 aggcattgaa attagtggat tatcataaga tcgaactaaa tgacaaagaa aaaactgttt
     6841 atctaaaaga aaaaggaatg atcatcgatt taggtgcaat cgccaaaggt tacattacgg
     6901 acgaagtagt gaaagtcttg aaaaaacaag gagtgaccac tgccatcgtt gacttgggcg
     6961 gtaatgtcta tgtcttaggc cacagcccaa gaggcgaaaa ccaagactgg acagtcggca
     7021 tccaagatcc gaacatggct cgcggatctg ttttaggttc tattaaagaa cggaacaaaa
     7081 cattagttac ctctggaatt tacgagcgtt atttagaagt agatggcaaa aaataccatc
     7141 atttgtttga tccaaaaacg ggttatccgt tcgacaatga tatcgcaagt gtcacgatca
     7201 taacagataa atcaattgat ggtgacgggt tgtctacagc agtcttttca atgggtgtga
     7261 aaaaaggact ggaatacgtc gaaagcgaat tgaataacgg gacggaagcg atcttcgtga
     7321 caaaagacga taaagtatat gtgaccgatc caatcaaaga caccttcaaa ttatcggagg
     7381 actcccatta cacgatgggt gaccgttctg aactaaaata aggagtacga accatgacac
     7441 taaaaatctt tttagaagta gttgaaatac gaacaaaggt ggctagtgta tttccttttg
     7501 cggtaggggt attattctct atcgcttttt ttcatgagat acattggcta aacacttttt
     7561 tattttttat tggaatgcta gtttttgatt tagcgactac cgctatcaat aattacatgg
     7621 attatgaaaa ggctcattct gaagtatata agtatgaaga aaatgtcatt ggccgcagaa
     7681 atctatctcc tgtattgatt cgaaacatga ttttagctat gattgcattt gtttttgtta
     7741 tcggcgttat tctaacgatc aatacgggtt ggcttttact tttgatggga tttatctgtt
     7801 gttttattgg agtattctat acattcggac cgattccttt atctcgaatg cctttagggg
     7861 aaattttcag tggggtgact atgggccttg gaatttttgc catggtcgtt tatgtcaata
     7921 cattcggttc agatgtctat gatctgacag tcaacttttc atctggacaa tttgctttgg
     7981 taggaaatct ttgggctata gcagcaatcg tattagcttc cttacccttg gtgtttacga
     8041 ttgccaatat catgttggct aataatttgc gggatcttga ccgtgatatt gaaaatcatc
     8101 gctatacact agttttttat atcggacgtc ctgcgggaac agtcttattc cagctattga
     8161 tgtatgcttg ctatttcgtg attttaatgg ggttgttgag cggtatctat gaatggccga
     8221 tcttggtcac cttcttgacc ttgccaattg tgaagaagca tttggatcaa ttcaagcaaa
     8281 cgttacccca gcctatcagt ttcagctatt ccatcaaaaa tatggtctta ttcaatgcca
     8341 gttatgtatt ggggctatta ctaacaatca ttatgcaagc catttaacag catgaagagg
     8401 ttgtgaaaaa actgccctgc atcaagtaat aagaacagcc aaataaaaat agctccttat
     8461 atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttatcagg
     8521 aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa aatggctttc
     8581 cacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca ccgtttatca
     8641 gaggttgtaa aatcagtgtt ttgtcctgag tattagagaa acaaatggaa aaatagttcc
     8701 tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca ctatttgttc
     8761 ggaaaaagtg actaactatg acacaatttt tttcacagtc ccttttttca cttgatttga
     8821 ttcgtaccag cgaacttgac gagaactttc ttggtttggt aactatatgg tattctttta
     8881 acatcctcgt ttgcttcttc taatttattt atacaaagtc acactttttt aaacatctgt
     8941 tcttcgatac atttctattt cttttaaaca tcgataccaa tattttgaac ttaagagtgc
     9001 ataccaaatg gattcattta ctcttcgtca gttacgaaga taatcaaaat ctccaacttc
     9061 cctacttatt tcgttttgag aattatcgaa gcaatcttac ataaataact aaaaaacaaa
     9121 aaaccagaaa atcagtatac tgattttctg gttttacatg gtttgttcaa taaaaagctg
     9181 ttggaactgt taatattaaa ttatcgcatc gttacgaatt cttccgctcc tgttggatgg
     9241 atggctactg tattgtcaaa gtcttcttta gttgcaccca tcttgatagc cacggcaaaa
     9301 ccttgcagca tctcatcgac accaacacca atagcatgta agccgacgat tctttcttct
     9361 tttccgacac agatcaattt catttcgcat ttttggcgat aatcatttaa tgcaaagtac
     9421 attggggtaa agcgtgaacg ataaactttc aattcatttt cgccatattt ttccaatgct
     9481 tcttcttctg tcaaaccaat tgttgcaatt ggtggatgcg taaagacgac tgtcggaaca
     9541 agtttatagt caagataaga atctttcttt ccattaaata aacgttctga taaacgtcta
     9601 ccagctgcga ttgcaacagg agtcagatct aatttaccga tcacatcacc aacagcaaaa
     9661 atatgttcat ccgttgtatt ttggtattta tctactttga taaacccttt tttgtccaat
     9721 tccacatttg tattttccag acctaatttt tcggtattcg gtacacgtcc accggcaaaa
     9781 agtacacaat caccagttaa ggtttcacca ttttcaaata gcaccgtata ttcgtcattg
     9841 tttttagtaa tttctttagc aatatgatgt gcgtaagttt ttattccagc ttcttggtac
     9901 atttggatca aattgtccga aagcatttta tcaaacgttc taagtggtcg ttcttttcga
     9961 tatgcccaca tgacttcact acctaaacca cgaaatacac ctgaaagttc agccgcaatg
    10021 tatccagcac ctaaaacgat tgctctctta ggtaactctg ttaattcaaa aaatccattt
    10081 gaatcaattg cgtattctcc accaggaatg gtcattttac ttgcacggcc tccagtagcg
    10141 atcaagattt taggagcacg ataggtttca ccgtttactt cgatcgtttg actatcgata
    10201 aaagtagcgt aaccttttat agcctcgaca tgattgctat ctaatcctct ttgataagca
    10261 ccgtgtagaa aatcaatata tttttctcgt ctttctacta gaccggcgaa atcaacattt
    10321 tttaaatctg cttggatacc gtaacttgag gcatctcgtt caattgtttc caaaatcgta
    10381 cttgcttgcc acataacttt tttaggtaca caaccgacgt tgacacatgt tccgcccaat
    10441 tcattcgctt caatcagcaa aacttttgcg ccatgcatcc ctgctctgtt tgctgaagca
    10501 ataccgccag agccgccacc aattacgatg taatcatata tttgcatttt gttcctcctt
    10561 attgaagaga tcaatcaaaa cctttattta acgatccttt ttctacagaa aacaagtgta
    10621 acacttttct tgaattttgt atgcgaagat gcttaaaaca gggatatatc agctttttcc
    10681 ttatttacgt tttcggatca tatctccagg tttcaccggc tgtgcgactg gcatagttaa
    10741 aatcatcatt ggatttggtg ctcggtcaat ggattcgcct tcttcattat gcataacttc
    10801 aacggtttga ctgaaatgac tgaatcctgg accataaaat tcgatttcat ctcctacaga
    10861 aaacaagttt ctctgacgta tcgtagcaat ctgtgttgcc tcatcataag ataatacttc
    10921 accgacaaat ttatatactg ggattttacg acgttcaccg aataattgtt cgttttcagt
    10981 tggtgtattg tagtaaaaac ctgtagctaa ttctcgttgt gctactttcc ataactcgtc
    11041 gatccattct tgcttgcaga catagttttc aggatcttcc atataactgt ctacagccgc
    11101 tttatagaca tttgacaccg tagatacata atggatcgat ttcattcttc cttcgatctt
    11161 gaagctgtct acgccgtttt caatcaattc tggaatatgt tcgatcattg acatatccac
    11221 tgcactcatc gagaattctt cttccacttc gcctgtatca gttaaactgt ttcgttcttg
    11281 accaaaaggc atatcgaaca atccatactt ccagcgacaa gattgagaac atccgccacg
    11341 gtttgcatcc cgcatcgaca tatggttgga caatgtgcat cgaccagaat aagagataca
    11401 catggcacca tggataaatg cttcgatttc ggtatccgta tttttacgaa tctctgccac
    11461 ttcctccatt gaaacttcac gagctaaaac gacacgttcc aacccttctt ctttccagaa
    11521 ttccaatgtc tcaaaattag ttgctgaagc ttgagtggat aaatggatcg gcagacctgg
    11581 ggcttctgtc gcacaaattt cgatcaaagc tggatcagaa acaatcactg cagaaatacc
    11641 gacatcacgc aaactgcgga aaaattcccc tgctccatct tcatttcctt catgggtgac
    11701 catattagca gcaacgtata ccttcgcatc atgttctttt gcaaaagcaa ctccttcagc
    11761 catttcttca taagtaaaat tacctgcacg gcttcttaaa ccatatgcat tcccgccaat
    11821 atatactgca tctgctccat aatggatcgc ggtttttaac ttttccagtg ttcctgctgg
    11881 cgctaggatt tctggtcgtt tcaattttct ttttactgtc atttctttca tcatactcct
    11941 tatttgatag cagaaggatc aatataaaag aatcctgtat ctaattcacg gttttcagga
    12001 tgcaatttac tgatttcgct agtcattttt tccgcttttt cttgatccca gctaccttct
    12061 aaaatttgtt tttttgcttc ggcgaatagt cgagtgatgt ttacgaattt ttcgcctgga
    12121 gtaaataatc cgtccaattt ccatgtacag taatgatgct gataaagttc attcaattca
    12181 ttcgagagat tcagatcatt gctggcaaaa atatgtgtgc catgactatc ttcataaata
    12241 gaataatgtg tctcttcttt tttaggttcc gagataaaaa gacctctttc acgatctttt
    12301 tgttcgtctt gtttcgtata gttatagtaa ttctgtagca gtggacgttt ggattgatgg
    12361 atgcatgtag caccatacac aagggtttct acaggaatat cgagtttttc ttccatcgcc
    12421 ttcatctctt caaaaggaac ttcacgagca agtactgctc caactgcgcc ttttttcgcc
    12481 cagaaattga tttgacgtgc gctcgttact aatgtttcag catcataaat atacggtata
    12541 cgcaattcag gatttttttt catcgtataa ataatacctg tatcaccaac tgtgatcaca
    12601 tctacgttta tttcttttaa aaaagccaaa tattcaggta catttttcat tttttcagga
    12661 tgaatgatcc catttacagc aatattcgct tttttaccat acttgtgtgc taattctacc
    12721 aattgtcgct gttcttctcg tgtaaatgaa gcaggcagac gcaatccaaa ttgctcttca
    12781 ccgaaatata gtgtatctgc acctgcagca agcaactgtt ctgcttgttc aatagtttct
    12841 actgtggtta tgagttcgat cataattttc tcctcctaca acttcataat agtagcatag
    12901 ctctaaaaac tttccaatac ttcttctatt taaagtcgga tttttgaaaa ttatttcaca
    12961 aaaaatcaaa ataaagttcg atatattctg tccaaatata aaaagagatt gtgacaaaaa
    13021 tcttgtcaca acctcttaat caagtcttac tattataaaa attcagctac acaggccaag
    13081 taactatagg gaacctactg tttccttttt aggtccgcga acattcaatg tgatcttacc
    13141 ttttgaagca cctactgtta cgtggtcacc taaacgaatt tctccgccaa gcaaagcctc
    13201 gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg attggacgtg caccatattc
    13261 aggatcaaat ccagctttcc cgatcacatc gattgcagat ggtgtgattt tcacttgaat
    13321 atcttgttct ttcaatcgtt tgataatcgc tttgctcatg atttggacga ttgtatggat
    13381 ttcatcttta ctcaatgaat ggaagacgac agtctcatcg attcgattca agaattcagg
    13441 tcggaaagct ttctttaact cttctaagat acgtttttgc atggctttgt gatctttagt
    13501 aacatcttgg acattgaatc cgacattttt ttcttctcga atttgtgtcg ctccgatatt
    13561 tgaagtcata atcatgattg tatttctaaa gtccactttt ctacctttag aatctgttaa
    13621 atgaccatca tccaatactt gcagaagtaa attgaaaacg tctggatgtg ctttctccac
    13681 ttcatctaag agaatcactg aatatggttt ttgacgaatt ttttctgtca actgtccacc
    13741 ttcatcatac cctacgtatc ctggaggaga cccgattaat cggcttgtac tgtatttttc
    13801 cataaattct gacatatcaa cacggatcag ggcatcttca cttccaaaca tgacttctga
    13861 aagtgctttg gccaattctg tttttccgac accagttggt ccaaggaaca tgaatgaacc
    13921 aattggacga tcaggatctt tcaatccgct tcgtgcacgg cggatcgcac gtgaaactgc
    13981 tttgacagcc tcatcttgac caacgacacg ttcatgcaga agtccttcca gttctaacaa
    14041 gcgttcgctt tcttttttct ccaactgctg tagagggaca ccagtccact ccgatacaac
    14101 tgtagcaaca tcttcttctg ttacacgatc agaataacct tgctcctgtt ttgcttcaat
    14161 tgcagaaact ttttgcagct ttttacgtag tgctttttct tgtctgcgta attgagcagc
    14221 attttcaaag tcttgttttt ggatcgctgc ttccttttct tgtactagtt catcgatttc
    14281 tagtttgatc acagttgatt ttgttaagtg atcggtttga tccaaacgga ctttcgctgc
    14341 tgattcatcg atcaaatcaa ttgctttatc gggtagttga cgagaagtga tataacgaac
    14401 agaaagattg actgctgctc gtacagcttc atctgtgatc tccactccat ggtgctcttc
    14461 atatcttgta cgtaatcctt gaagaatcac ttctgcttct tcaggagttg gctcatctac
    14521 ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt tcgatatatt tttgatattc
    14581 atctagggtc gttgctccga tcgtttgtaa ttctcctcga gctaacgccg gtttcaagat
    14641 attcgaagca tcaattgctc cttctgctcc acctgcacca attaaggtat gcaattcatc
    14701 aatgaataag attacttgac catcattata gatttcgtcg atgacctttt tcattcgatc
    14761 ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat cccatatcaa gcatcatcag
    14821 gcgcttttct tgcatgtctt ctggcacttc acctaaaatg attttttgag ctaacccttc
    14881 agcaatggct gttttgccta caccaggttc acccaccagt acagggttat tctttgtccg
    14941 tcggctaaga atctggataa gccgtttcac ttctctcgaa cgaccaacaa ctgggtccag
    15001 cttgttttct tttgctaatt tcgtcaaatc acgtgctaat gaatctagcg taggtgttcc
    15061 ttgcggtgtt tgccgtttat cttgtacatt tacatttcta cgacgattca ttccttttcc
    15121 agcagaatca ccagctagcc ccattttttg tttcagaact tgacgggctt ttgaaaggct
    15181 gatacctaag ttgatcatga ttcgagtagc catgatactt tcatctttta ataagctcat
    15241 caaaatatgt tctgttccaa ctttttgaga accagttttc ttcacttcgc tgctagctaa
    15301 agcaaaaact tgtttcattc ttggtgaata tggaagaaag actcctttag ggtaagaacg
    15361 gataccacca taaccaatca gatgttcaat ttcttcataa atttccgttt cattcaaatc
    15421 catttcacgt aatacttttc cagcaattcc attttgctcg accactaagg ctagtaaaac
    15481 atgttctgaa ctaactgatt gatgtttcaa ccgttttgct tcttcttgtg caatttctaa
    15541 tactgcttgg gctctttcag taaaaagttc ttccatctaa tctcacttcc catcttcata
    15601 acttaaacgt tccagaaatg ctcggagaat ccgagcacgt gtatgatctt catattcatt
    15661 gaaattcaat gtgttttttg caattgcact caacatcaag tttccttctt tttttgtaat
    15721 tatttggtct tcataaagtt tttgaataat agcaaaagca tttttctcac tgattgtatc
    15781 atcaaataac tgatttatct gctcaagtaa ttgtttttta tctgaaatcc ttacttttgc
    15841 aattcgtata taaccaccac caccacgttt gctttctact agataaccac gttggatggt
    15901 aaaacgggta ttaatcacat agttgatttg tgaaggaaca cagttaaaga ggtctgccat
    15961 ttcaatcctt cgaatctcga tcatttcact attttccaga attttcttta tatatgattc
    16021 aattaaatcc gaggtattct gatgtgccat cttattcatt ccctttgact aatattgacc
    16081 ttaattataa cgaatttgat aatagaaaga aagaagaacg ctttttgtta aataaatcct
    16141 ttagaatcaa caaaaaaaaa tacggaatat actattccgt atc
//
LOCUS       DAZKNH010000056        16150 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000056 DAZKNH010000000
VERSION     DAZKNH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.056"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACG8MG_001718"
     CDS             226..894
                     /locus_tag="ACG8MG_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFQ0375761.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1414)
                     /locus_tag="ACG8MG_001719"
     CDS             complement(1094..1414)
                     /locus_tag="ACG8MG_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375762.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1451..2833)
                     /locus_tag="ACG8MG_001720"
     CDS             complement(1451..2833)
                     /locus_tag="ACG8MG_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0375763.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACG8MG_001721"
     CDS             complement(2989..4176)
                     /locus_tag="ACG8MG_001721"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFQ0375764.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACG8MG_001722"
     CDS             complement(4183..5214)
                     /locus_tag="ACG8MG_001722"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0375765.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="ACG8MG_001723"
     CDS             complement(5230..6684)
                     /locus_tag="ACG8MG_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFQ0375766.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6859..7764
                     /locus_tag="ACG8MG_001724"
     CDS             6859..7764
                     /locus_tag="ACG8MG_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFQ0375767.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACG8MG_001725"
     CDS             7855..8532
                     /locus_tag="ACG8MG_001725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0375768.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACG8MG_001726"
     CDS             8529..8699
                     /locus_tag="ACG8MG_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375769.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACG8MG_001727"
     CDS             8813..9709
                     /locus_tag="ACG8MG_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0375770.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACG8MG_001728"
     CDS             complement(9764..11134)
                     /locus_tag="ACG8MG_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0375771.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACG8MG_001729"
     CDS             11372..12118
                     /locus_tag="ACG8MG_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375772.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACG8MG_001730"
     CDS             12115..12954
                     /locus_tag="ACG8MG_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0375773.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="ACG8MG_001731"
     CDS             12955..13905
                     /locus_tag="ACG8MG_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0375774.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="ACG8MG_001732"
     CDS             14046..14798
                     /locus_tag="ACG8MG_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375775.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="ACG8MG_001733"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="ACG8MG_001733"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFQ0375776.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5240 a         3010 c         3242 g         4658 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gatctactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
    16021 actggttttt ttgtattttt tacatttttt taagagaatt tgataaaaat caaaaatttt
    16081 tttgcttttt tttatggatt attgcagtga atgttacaga atctgcgttt ttcattcaaa
    16141 aataaaaaaa
//
LOCUS       DAZKNH010000057        15743 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000057 DAZKNH010000000
VERSION     DAZKNH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.057"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..722)
                     /locus_tag="ACG8MG_001734"
     CDS             complement(24..722)
                     /locus_tag="ACG8MG_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFQ0375777.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ACG8MG_001735"
     CDS             complement(715..2067)
                     /locus_tag="ACG8MG_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFQ0375778.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ACG8MG_001736"
     CDS             complement(2290..3690)
                     /locus_tag="ACG8MG_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0375779.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ACG8MG_001737"
     CDS             3851..4588
                     /locus_tag="ACG8MG_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFQ0375780.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ACG8MG_001738"
     CDS             4713..5192
                     /locus_tag="ACG8MG_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFQ0375781.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ACG8MG_001739"
     CDS             5189..5956
                     /locus_tag="ACG8MG_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFQ0375782.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ACG8MG_001740"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ACG8MG_001740"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0375783.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ACG8MG_001741"
     CDS             complement(6859..7461)
                     /locus_tag="ACG8MG_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0375784.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..7797
                     /locus_tag="ACG8MG_001742"
     CDS             7567..7797
                     /locus_tag="ACG8MG_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375785.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7788..8036
                     /locus_tag="ACG8MG_001743"
     CDS             7788..8036
                     /locus_tag="ACG8MG_001743"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0375786.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8142..8726
                     /locus_tag="ACG8MG_001744"
     CDS             8142..8726
                     /locus_tag="ACG8MG_001744"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFQ0375787.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ACG8MG_001745"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ACG8MG_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFQ0375788.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ACG8MG_001746"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ACG8MG_001746"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFQ0375789.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ACG8MG_001747"
     CDS             10627..10806
                     /locus_tag="ACG8MG_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375790.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ACG8MG_001748"
     CDS             10828..11853
                     /locus_tag="ACG8MG_001748"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFQ0375791.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /gene="glyA"
                     /locus_tag="ACG8MG_001749"
     CDS             12028..13272
                     /gene="glyA"
                     /locus_tag="ACG8MG_001749"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFQ0375792.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="ACG8MG_001750"
     CDS             13425..14372
                     /locus_tag="ACG8MG_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFQ0375793.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="ACG8MG_001751"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="ACG8MG_001751"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFQ0375794.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="ACG8MG_001752"
     CDS             complement(15274..15516)
                     /locus_tag="ACG8MG_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375795.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="ACG8MG_001753"
     tRNA            15637..15709
                     /locus_tag="ACG8MG_001753"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
BASE COUNT         5350 a         2823 c         3195 g         4375 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt tta
//
LOCUS       DAZKNH010000058        15444 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000058 DAZKNH010000000
VERSION     DAZKNH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.058"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1016..1357)
                     /locus_tag="ACG8MG_001754"
     CDS             complement(1016..1357)
                     /locus_tag="ACG8MG_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0375796.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1357..1587)
                     /locus_tag="ACG8MG_001755"
     CDS             complement(1357..1587)
                     /locus_tag="ACG8MG_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0375797.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1874..2212
                     /locus_tag="ACG8MG_001756"
     CDS             1874..2212
                     /locus_tag="ACG8MG_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375798.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2417..2979)
                     /locus_tag="ACG8MG_001757"
                     /pseudo
     CDS             complement(2417..2979)
                     /locus_tag="ACG8MG_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(3711..4292)
                     /locus_tag="ACG8MG_001758"
     CDS             complement(3711..4292)
                     /locus_tag="ACG8MG_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0375799.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4475..5101)
                     /locus_tag="ACG8MG_001759"
     CDS             complement(4475..5101)
                     /locus_tag="ACG8MG_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0375800.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(5123..6454)
                     /locus_tag="ACG8MG_001760"
     CDS             complement(5123..6454)
                     /locus_tag="ACG8MG_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFQ0375801.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6609..7010)
                     /locus_tag="ACG8MG_001761"
     CDS             complement(6609..7010)
                     /locus_tag="ACG8MG_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0375802.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(7096..7302)
                     /locus_tag="ACG8MG_001762"
     CDS             complement(7096..7302)
                     /locus_tag="ACG8MG_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375803.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7397..8899)
                     /locus_tag="ACG8MG_001763"
     CDS             complement(7397..8899)
                     /locus_tag="ACG8MG_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFQ0375804.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8917..9372)
                     /gene="argR"
                     /locus_tag="ACG8MG_001764"
     CDS             complement(8917..9372)
                     /gene="argR"
                     /locus_tag="ACG8MG_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0375805.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9470..10087)
                     /locus_tag="ACG8MG_001765"
     CDS             complement(9470..10087)
                     /locus_tag="ACG8MG_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0375806.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10308..11681
                     /locus_tag="ACG8MG_001766"
     CDS             10308..11681
                     /locus_tag="ACG8MG_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0375807.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11756..12868
                     /locus_tag="ACG8MG_001767"
     CDS             11756..12868
                     /locus_tag="ACG8MG_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0375808.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12940..14577)
                     /locus_tag="ACG8MG_001768"
     CDS             complement(12940..14577)
                     /locus_tag="ACG8MG_001768"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFQ0375809.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14745..15353)
                     /locus_tag="ACG8MG_001769"
     CDS             complement(14745..15353)
                     /locus_tag="ACG8MG_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0375810.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4278 a         3168 c         2693 g         5305 t
ORIGIN      
        1 aaccaacagc aaaaaataca atgaaaaaca aacttggaac taatgtatca ttaataaatg
       61 ttgaaaaatt cagcaactca ttactgtatg attttagcag cccaaccaac atatacccta
      121 taaaagctaa taagatggtt aggaatcctt tagaattttt actcattcat agacacttcc
      181 ttaaataact tttattatag aaggtattgt aaataataat attgacaaaa agtgactcaa
      241 aataaaaaaa accttacatt atactggaat gaactgtatt gatagtgtac ctaaaagctc
      301 tactgacata agatcgtgta atcggtaaag taattttatt aattatacgg gatgtaatca
      361 attagagaat aaactaatca taaaaggtac ttttacattc ataattgtcg ctttgagatt
      421 ctactttggt tcactatctg acccaattgc acattttcga tcattacaag tattggtgtc
      481 gttagaacaa ctaaataaaa actagcggta gatttttcag aatctatgct aatttctgta
      541 tggatgttcc agtgatggca aatttctctt ttcattgttt ttcttgtcgc atgatatgcc
      601 tttttgtgtt attcagttaa actatagaaa ttctttccta gacagatctt gatcccaaat
      661 tttacgtttt tgagcatcga tatagtttaa aatgacatta ctcatatcta aagagtttag
      721 attttttagg actaaaactg tcagttgttc tttttcttct ttctgttaaa gtattgtcgc
      781 gacttttcgt atacgttaag atatgtcgtg aaaaaacaag ttttgtgaca caaaaaaacg
      841 aatgacttga ggaaatcacc tcaatcattc gtttaaaatg ctatatattc tttagtttaa
      901 gatacggaaa ttaatgccat cgaccacact agtagaatga acgacattta cttggccttt
      961 ttttaatttc gttatgttta gcatataaaa atattataga gtttacttac aatgcttaaa
     1021 acatatactg tataatctga tcaattttag ccatgtcttg caatggtaag ttttctattt
     1081 ttttgagttt tcgttcctta aaatcaagag attttaattg tgtaataatt acttgtcctt
     1141 ttatatctag ctcttcaggt aatgagtaac gtgtcggtaa attctttata gtggaagtaa
     1201 ttggacatat gaccgaaaac attgttttac ggttaaattc ataacgagag acgactagtc
     1261 ctggtcgtct tttttgaatt tcttttccta cggagggatc aaaatcaatc catacaatat
     1321 cacctttttt aggaatataa attctttctt ctgacactat aaaatttctc tcccttcagg
     1381 agttaaatct tcccattcgt ctttctcata atactcggcc tcttccccac cactaaatgg
     1441 atcttcaatt tttggaacta acgtaattgt tccatcatca gaatacacaa cgatatattc
     1501 ttggttttct gaaggtttct ttccgttatc agaaggtaaa gttaccacta cagaacttcc
     1561 ttgtaatcta gattttgtaa caagcattat catcactccc ttaataaaag tatactatat
     1621 aacactaagt aacgcaaaat tcccctttgt aaactatcta gaaaattgtt ttgtttaaag
     1681 tatatcttat tggatataaa tagaaataaa ttgtagccag ttcatttttt ctttaactaa
     1741 aaaaataatc caagaaacat tgaacctatg gatccaaaag ctacccaaat actatagcta
     1801 accccccgct accttcaatt ttgcagataa aacgctttgg gagaaaattt caaagacaac
     1861 aggccatcct atcatgcccg attataaaca aaaagctggc tattcctttt gctctgacaa
     1921 atttcaattg ggaaagttca cgaaactagc aggtgaagaa gtttcaactg ttcggatcaa
     1981 agaatgcccg cttcaaatag aaacattggt tcaacgaatt tcaactagac aagactttgc
     2041 tattgtagaa cgtgagatcg tagctatttt tgtagaagaa gggattttac atgatccaac
     2101 tcatatcaat gtggataaat ggcaacctct tatctataaa ttcagagaat atacaactgc
     2161 caaccagtcg ttaactttga attttagatt ccaagaattc aaagagtcat aatgataaac
     2221 atcgttaggt ataaataaaa aagactcgca gcacgagtaa aaaacaaata gaagctatac
     2281 attagaaagg tcgactgtga ccctatagat gcagtatatg cagcttatga gcatttcttc
     2341 tgttctcttt gtttttggga tctgtgctga agtctttttc gttttttgcc gctctttctt
     2401 tgaaaaatcg attatgttat accttctcca ttactaattc aggcatctgc tgagtaatga
     2461 tttttcgcgc aaaagaaata tctgttaccc aaggttcgat ttgttcttca gtcaagacaa
     2521 gcggcattcg gtcatggatt ggtgcgactg tgcggttagg ttcagtagtc attatgattg
     2581 attctgccat tttttcatcc tgatggctac gataaaaacc gccaatatac actatttttt
     2641 ccttatttga aaacataaat ttgtcttttt cttcattcca ctcattggga aaacacaacg
     2701 attttgcaaa aatggttgat gaaaggtctt tttttcttct actgtctctg cacgggcatt
     2761 aatcatcaat tgtccttttt taaatccggt aaatccccac ttcatgattt ccggagtgag
     2821 gatgttttct tcctcttttc ttaacgtcac aacaagattt gacggaaaaa cttctccagc
     2881 tgctaattct gctgcctgct tcggttgttt cgtctgggct tctcgataat aattctgaag
     2941 gagctctgac tctagatcaa aaaaataacg accacacatc ctaatccctc ccaatctttt
     3001 tttctatcat acactgcaac aagaagttcg caccaacaaa acgctttcaa tttttgtaga
     3061 actaaaaaag ttgtgacaag tgacaaactc tccagtgaat ggtattctcc tgccaactcc
     3121 tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ctttttgaaa ttgttccttg
     3181 tgactcggag tgaaacggca ggcttacgac ctctgggtaa acggtgttca aaagccagtt
     3241 tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc tattttcaca aaatttttct
     3301 taatactcga aactaagcga ctttttcaca acctcttcct taaacggtgt tcaaaagctt
     3361 gcatctgcgg taataagccc aaccaaacga aaaatatgag cagctatttt tatttggctg
     3421 ttcttattac ttgatgcagg gcagcttttt cacaacctct ccactaaacg gtgttcaatc
     3481 agctaacctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg
     3541 tttctctaat actcaggtca aagcgctgat ttcacaacct ctaatacacg gtgttccaga
     3601 agtagcttct tcgaaaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa
     3661 attccttctt atttcttgaa gctgaccact tctgtcacaa cctctaatta ttaaagaacc
     3721 ttttgcgaag ttaacaatct ttctttgatc aattgaaaat tctgcctaag tagtgataga
     3781 tcctgtgtga ctttcaacaa taagatactt ccctggattt cagcaatcaa cgctttggct
     3841 tgacggtctg cttcttcatc ggacagtttt tcttcagaaa gtgccaggat attttgtttc
     3901 gtatacttga cccattgatt gactaattct agtagcaaca gacggtattc ttcatccact
     3961 gtactcaact caataatcaa attacctaca ggacagccat gataagtgat ctgtgtctca
     4021 tgatcagcga aaatccagtc caacatttct tctaatgctt cttctggctg ttttccagca
     4081 tttaagatgt cttcgaacaa ttctgtctgc catacgtcca tatgcgcatg gatcaccgct
     4141 agacaaactt ctttttttga agaaaagtaa taataaaatt gaccttttcc tgtatcagaa
     4201 gctgctagaa tatctcggat tgacgtttca tgatagcctt ttttataaat aagatctttg
     4261 gtcgcttcca agatcttttc ctttgttttc aatgatattc tcctttttca tttctcatag
     4321 tcctatatta tacagtaaat tcatcatttt ttcttcttaa tcgtttaaaa aagactgtgc
     4381 caaattatct gacacagccc tcactataaa actgttctca tatagctaga tgttcattga
     4441 ttcaagaact gcgaggacaa aagcaattcc tatcttatga tagttgctct aatacttcct
     4501 ctgttgtcat cacttgatct gcaataaagc caaagttcgt caacgctgca tccattgctt
     4561 cttggccagg agcaccaatt gcatctgtcg ctaccacaac attgaatcca ctctctacca
     4621 attcacgcat atgggaatcg ctacacaagt ttccgttcat tccaccaaga attactgtgt
     4681 cgattccatt ttttctcaat tggagcgcca aatcattgct ttctggtcca aagattttat
     4741 gaggagagct gatgatcgta ttttcatcca aataaggttt caattcttcg ataaaatcgg
     4801 ctcctgaacc ttcttccact gattcatatt gactttttct agcaaacatc tgctgctcaa
     4861 gcatcatatg ttctcctgca ccgccaaatt gccatttatg gtcatgtggg taaaaataat
     4921 gaggagaaat aaagagtttg tatccttttt cttttgcttg ttttaccatt tcaatcatat
     4981 tttcgatcgt attgtatttt tctaagatat cttttgtcaa accaaagcca ccgcctgctg
     5041 gtttcaagaa ctcgacttgc gggtcagtga gaactactgc tgtattttta ttattgaaca
     5101 tggtggattc tccattcgtt atttatttta atacatcatt tagagcttca ctcattgtcg
     5161 gatgagaata gatttgatct ctcaataacg tataaggaag ttttccattc atagctagcg
     5221 aaatcaagtt gatgacttca tgagaatctt ccgcatagat acttgcccct agaatcaagt
     5281 cagtttcagg gtcgactaaa actttgaata cacctttagg atcttctaat acttgtgctt
     5341 tagggacacc gcttgtcata tacttgaata atttgtaatt gacgccttgt gctttggctt
     5401 gtttttctgt taaacctaca ttggataaag tcggagtgat aaatacactg taaggcacag
     5461 ctttccggtt attcgttgtt cgtttattct cgcccttcaa ctgatccata atgattcgat
     5521 aatcatctag agagatataa gtgaactgca atccgccttt gacgtctcca atcgcccaaa
     5581 tgttgtctgc atctgtacga aggtattcat ccacagcgat tgctccgcga tctgttagtt
     5641 taacatctgt attttctaat ccaagtgatg ctgtacttgg ttttcttcca gttgcagcca
     5701 aaacttttga cgcttggatt gttacttctt ggccatcttc tgtaaaggtg acggcagctg
     5761 ttgattcttg gtctgctact tgatctacag atacacctag atggaattcg attcccgcat
     5821 ttgtcatgtc ttctaaaatc atttgcgcga tatcttcatc ttcacgtgga agaaattcag
     5881 aatgggcatc aagaacgaca actttagatc cgtattcatt gaacattgat gcaaattcca
     5941 agccaatata tccagccccg atgatcacta atttttccgg taattgttcc tgatccatcg
     6001 cttgcgtaga atcaatcaag tagcgacttg tattcaatcc tttgattggt aaaatgattg
     6061 gttcagaacc agtattgatg aaaatccgtt cgccttcaac aatcactttt tcaccattat
     6121 tcgtcacttc gatttgatgg ttagaaagaa aacgtgctgt accgtccatc actgtcccag
     6181 ttgcctcatc gctgatcata tgataatttt tggcgcgaag catgcccgtc aatttttctt
     6241 tccgagcaac agcctctgta aagtcgattc ctctttctcc attgaaaatc aatgatttag
     6301 aaggaataca gccgatgttg atacatgttc cgccatacat acgtgttgac ttttcaacga
     6361 ctaaaaccga ttcacctttt gaagctaacg ccttcgctaa agtcttaccg ccctttccaa
     6421 aaccgatcac aatattttga tatttttcca tcatttttcc ctcgcttctt ttttacctac
     6481 cgattggtac aaaaatatga taaccgataa ttgtctattc tccaaatcat ctgctttatt
     6541 tgtcaaaaaa aaccagacaa aaagcggtcc tttcacgttt ttagtctggt tcgatttcct
     6601 ttattcttct aaacaatcgt tacttctaca tcgatatttc ctctagttgc tttggagtaa
     6661 gggcagaatt catgcgcatc ttctgcaatt ttttgggctt cttcttcgct gatgccatcg
     6721 atatgcactt ctaatactac accgatctga tagccgccat catctaaatt ataaagagag
     6781 acacgtgctt ttactgtgga ctctgctgtc attcctgctt ctttgatcat atgttgtacc
     6841 gccccattga aacaagaagc ataacctgct gcaaataatt gttctggatt tgttccttct
     6901 gcatggattc ctggaggagt gattttcata tgcatcgagc catttggtgc ctcgacttct
     6961 ccattgcgcc cgccatggtt gatcattgct gtttcgtatg cttttttcat tgtactgctc
     7021 ctttcgattg accatttgct acttggctat aattatagtg tatgacaagt aaggcgcttt
     7081 ctacaaaaat tctgcttatt cgaataccag aatgccgtct tctactattt ttccaaaaaa
     7141 caaaaaaata ctactcgtga acaacagcgt catatttgtt ccaaaataaa tagcaatctt
     7201 ttctacataa tcttttatat gattgatttt aagatacata tataccagaa agttgacccc
     7261 ataaaccaat actgttaaaa aaataaccat attcgttacc attttttcct ccttttgctg
     7321 gtctccctac cagcactcga tttaaccata ccactttttt tgccgtccaa gcaactagcc
     7381 tttcaaaaag actggtttat tctgctgtta atgattcaag atcagaaaaa tgtccgaata
     7441 attttgctaa ttttactaac gccaacttgt tctttccgaa ttcatcattt tctatctgta
     7501 atactagtaa cggctcatgt aagcttaaac ggatcaaaaa ccagccgttt ccgtatggct
     7561 cttctagatc atatcgaata ccttcttcat tttccgggtc gaagttcatc cctttttctt
     7621 cttccaacag tcgcttcatt tcttctaata tcttgtttcc ttgttcgcgt ggttcatcgc
     7681 tgaacaattt taatcggatt tcttgtactt ctacgggctg tttcaactct ttgatttgtt
     7741 catctaatgt gatacctttt ctttttaatt ctggcaaaag catcaggatc tttgcgatga
     7801 catatgcacc atcatccaga aaatagtttt ccttgaatgc agcgtgtccg cttgtttcga
     7861 tggctagctg tgtatctttc ccaagacggt taagttctaa tgctttattg attacatttc
     7921 gatagccgga aatataacga acttggatgc ctcctttttc ttcaatgaat ctttgtagat
     7981 gagaagaagt aggggaattc gtcacgattc ttgttcctgg atgctctttt aatacgattg
     8041 tacttaatac tgcgatcaaa ttatttcgat tgatcactgt tccagaagca ctgacaactg
     8101 cagaacgatc cacatctgta tcgaaaatga tcccaagatc tgcttcattt gctaaaacag
     8161 cctgcttgat actttccatc gcttctttat tatcaggatt tgggatatga ttaggaaatc
     8221 ggccatctgg ttccaaaaat tgggaacctg ttgaatccgc acctaagcta ttcaatactt
     8281 tttcagcaaa gaatccacca gcaccatttc cggcatcaac aatgattttc caaccattta
     8341 gtggtttgct ttctagtgtt ccaatcccct ttttaatttt tgtgactaaa tcttgcgcat
     8401 aaggtgtcaa aaggtctgct ttttctacgc ttccagtttg ttcgcttttt attttttgct
     8461 gctgtgatag gataaaagca atatcttctt tttctgctcc tccttctgag gtaaagaatt
     8521 ttattccatt ataataatag ggaagatggc tagcagtcag cataaccgca gcatcgcacg
     8581 agaactgttc aaattgagtg gacataaaca tagcaggcgt agtagctaat tcgaaatcga
     8641 tgacatgtat cccttgttct ttcagacttt ggatcaacgc ctctttcagt gctggtccgc
     8701 ttatcctact gtcatgacca atcccaatcg tccatttttt atttggatcg gttgatttct
     8761 tctttttctt tatccagttt gctagtcccc agccgatctt ttttaccatg tctgtcgtca
     8821 agtttacttc atatccttca actggtaatg cgattcctcg aatatccgat ccattttgca
     8881 gcgctttcat atctttcata taaaagtcct cctcatttat tccctcattt tttccagctt
     8941 ttctgcaaat cgctgtgctt cttcttctga gcggcaaata ataatgaatg tatctacacc
     9001 ggccatcgtt ccgacaactt cagggtaagc cacttcatct agccaattcg ttaccacatc
     9061 ggcaccattt cgttcagtca atacgataac catgaactga acagactgga tacgcaaaac
     9121 ctcgcgtcta attgctgtac gcaaacgttc ttcatttaaa gaaacagatg gctggttaaa
     9181 taatgtatag cgtactattt tgttttcatt ttgacttttt actaacttca tttctcgaat
     9241 gtctcgagaa atcgttgctt gagtcacatc ggcgcctgct tcatgcagcc gcgcaagcag
     9301 ctcttcctgt gtcccaatag gttgttctgt aatgatctgg cggatcagtg cttgtcgctg
     9361 tttctttttc atattagaca cctctcaatg ttgattatag caaaagataa gtaatatttc
     9421 ttcatatttt ggtatttttt acttttattt tttgttttca ttcaggttgc taaccttttt
     9481 ctattttaac acgcaaaatc gtcttcattt tttcaggtgc tgattttctt ggatcgctga
     9541 tgtaaatctc tttgtgttct ttagaagctc tttcatagcc ttcttttgcg atgaatgctt
     9601 ctaatttctc aaacgttgct ggttcttcgg catagggccc gacgtgtaag atttgggcac
     9661 aaagaccttc ttccatcttt ttgaatacta cttgttcttt caagtcttcc ggaagtttgc
     9721 tagaacgctg aatggcttct tgtgctatcg cttctgtaac aaagtcaggc tgtcggatca
     9781 tcaattgata agaaaaatgt tcttttttca tcactggttc atgcaaatac ttttcttgca
     9841 gtccccattg tccttccagt ggatagacag tatacatctg ataatcagga atcagccaat
     9901 tttttttagg cgacatgcgt attgcatagc tgactgcata tagacaggct actcttcgtt
     9961 taaagtcttc ctgattcggg tcgccgatcc ctctgatgct aaagaatgat tttttaggaa
    10021 cctctagtat ttgcgggctt tttttcatac tatagctctt gtctttacgc cattcaattt
    10081 tttccatttt tcttcctccg cgaaattcca tttagttatt tattatcata cctctttttg
    10141 agtgacttgg ctaatcgtgt tgacactatt tttcagtttg tgctagaata agtgaagtaa
    10201 ataaatcgta cagataacac gtttcggcgt gtcgagagag aggcggtttc tatttttata
    10261 atagaaatca ctctcttttt ttacgaaatt ttttagaggt gatgtaaatg acaaaacaaa
    10321 aacagctgct tttagtacct gcagtaattg caacagggat gatgtctttt gcgggcgtat
    10381 tgatcgagac ggccatgaat gtaactttcc caactcttat cagacagttt gatttaacga
    10441 ctgcacaagt ccaatgggta acaacgattt atcttttgat gatttcgatc attgttccgc
    10501 tgtctactta tttgaatcgc aattttagtc tcaaaaaact ctttttagct tcaaatctgc
    10561 ttttcttggc aggtataatg atcgatttct tctctcctac cttcatggtc ctattgtttg
    10621 gtcgattgct ccaaggtgca gcgacaggta tcgctcttcc gttgatgttc catattatct
    10681 tgacatttgc ccccctcaat cgacgaggcg cgatgatggg tgtcggtact ttgacaacgg
    10741 ccattgctcc agcaattggc cccacttatg gaggcattat gacttcacat tttacatgga
    10801 atcatatctt cctgtttctt gtgcccgttt tgtttctttc tttgattatc ggctggtttg
    10861 ctattcccga gatggcagta caaaaaagtg gttatttgga ttggataagc gttttaggaa
    10921 tttgtctttt gtttagtgga agtctaacat ttttgagtac gttgggaaca gtttccagct
    10981 ggattgcttt aatcatcggc ggtattggct ggttcctttt ttactatcga agtaaaaaag
    11041 tggagcatcc actgatccga ctgtctattc tttcaaataa aacattccgc ttgtttttga
    11101 ttggcttctt agtttatcag tttttattac ttggtgtatc ttttgtcttg ccgaatttcc
    11161 ttcagatcgt tcaaggaagt agtccttttg tcgctggatt agcgatgctt cccggcgcag
    11221 cggttggtgc tttgctttct cctttttccg ggaaaatgct ggatcaatat ggtccgaaga
    11281 agccaatttt tgctgggctg attctttctt tgattggttg gctagcatta ttcctactca
    11341 taggacacgc ttcagtgggt gtattgattg cttgtcatgt gttttatatg atcggtatcg
    11401 gattatctta cagcaatatg atgaccaccg gaatgaatgc actgaatgaa gaactacaag
    11461 gagacgggaa cgcaatgttc aataccttgc agcagttttc tggcgctgtg gctacttcat
    11521 tagtagcggt cattatcaat tacgtccagc atcatacttc ccataactat gaagtgtcta
    11581 ctacattagg atcaaaagca gcattaggcg tcttgttgct gttgcttctc gtaagctttg
    11641 ctcgattcgc ctattatctg ttctttgcta aaaaagcgta gattccatat acggcaggtg
    11701 ttgcatctgc cgtttttttg tgtgaaaatg gttgtttgta aaaggaggct tttttatgga
    11761 aaaaattgcc attatcggtg gcgggatcat tggtatgacc ctagccagtt acttagatac
    11821 atcaaaattc aatgttgctt tatttgatga agggacagga caggcaacta aagctagcgc
    11881 tgggattatc tccccttggt tgtcgaaacg cagaaacaaa caatggtatc gtctggctaa
    11941 agacggcgct gcttttttcc ctaaacttat aaaagatttc caacttgatc aaacgattta
    12001 cgcacaatca gggacacttt tgcttcgacc tgaaaaagat ttagctgaat tggctgttct
    12061 tgccgagaaa cgaaaagaag aagcgccaga aattggaaac atcgaatggc tttcacctga
    12121 acagacatct caagcacttc cgttattgaa accagcagtt tcactgcgta tcagtggtgg
    12181 tggtcgtctg gatggaaaag catatttaga acatcttggt cagcgtttag cacaaaaaca
    12241 aatggagcag atccaggcaa aagtcacttt gcataagaag agcgattctt ggctcatcaa
    12301 gggaccgttt ggagaataca cagctgatcg tgtaattttg acacctggcc cggcacttaa
    12361 gcctttatta caagaattag gctatgctgc agatatacgt ccgcaaaaag ggcagctctt
    12421 agcatttcag acatcttata agatgagtca agactggcct gttgctatgc ttgacggaga
    12481 agctgatttc attcctttca tggatggaaa gatcctttta ggtgctaccc atgaaaacga
    12541 tcaaggctgg gatttggctc ctactagcga agcttataag ggattgacta agagtacaga
    12601 acagttcttg actgatccag atcagataaa aaactggcca tacgagttcc gtgtaggtac
    12661 ccgtgcttat acgtcagatt tttccccatt tttcggcgta ctgccagatg atccaagctt
    12721 actggctgct agcggtctag gctcttctgg cttgaccaca ggtccttata tcggctatct
    12781 attagccaac ttcttaaata ctggtcattg gcctacggaa gattatcaaa aggacatctc
    12841 cacctatatt acgaaaaatc catcatagaa aaacagcttt ttcaagtaag gatcccgagc
    12901 gatattgcgc atggattact tcttgaaaaa gctgttattt tagttcaatt tctgaatcaa
    12961 attctccaaa ctttcttttg cttcatctgg caagagatct tttcctatct gagcttcaat
    13021 gaatcgtaaa cgaatatcaa gtcgttgttt caccagattt ttataggacg tatcctcaaa
    13081 agaaacagga aaatctggta taggtaaata gtggatgtct tcaaatggac caaacgactt
    13141 gaattctgcc gtttgttgca cgattctttc aacgattgac aatgtcgttt caggtttgat
    13201 ttttttatca tcgaaatatc cagtattcag gacatagcag gctgtttgct gtttcaaaaa
    13261 caactctcga aaagcttgat agtcttccct aagaggatag acacggaaag gattagcaaa
    13321 tggttcaatg actagctcat ctagctgtct ttttgtagtg atattttccg cgtctgatcg
    13381 ttttgttgct aatgttgcac caaaagacgc tgctaaaact gggtcattga tcttgataat
    13441 tggcggaagc gaatcatctt tcatgatcca aaaaacagca tcgatctttt cgctaagatg
    13501 atcgactcgg tcgggtgtcg cataacgtga tttgactgtc cgcccatttc cgtttcgtat
    13561 atctcctgtt actaggactt tttctccttt acttgtacat gtcacgccca cattctgaca
    13621 agtaaggaaa gaagaaatgg ccggatcatc agacggatag tcttgtgttt tatcaaagta
    13681 agaaggttcc aaggcagtag caaaaccatt ttttcgagaa ataacgaaag catcgtcatg
    13741 caagatcgaa acagagtgac cttccgtatg ttttgctaag gtaatcgttg attttccaga
    13801 accggacaac ccaaatacag ccatcgtata ggtctggtca ttactagcat attgtttcat
    13861 cccaccgtgg caggcaataa agccattgcg atttcccaat gcccaagcaa gcgtcaaagt
    13921 cgcttttttt aattctccaa agtaacgtaa tcctagcaca gccgctgtat tatggagagc
    13981 atcaaacaaa atgagtccat ttggaaatgt ttcatgtgtc cattcgggat cacaaaagat
    14041 ataaagatct ccctcatcaa aggaaacaga cttttgataa gtctctaaga tcttcgaatt
    14101 cgctggctga aaattcagca aataagaata caaattgttc tcaaatcctt ctggaatcag
    14161 caaatgactt tgcacagaaa attcttcaga taggccaacg attacttttg tatggtaaaa
    14221 ctggcgcttg cgactttgat acacagcttc tcttaaaatt ttctcgtatt tgctttgttc
    14281 cttgtctaag tcctcaaaga tccgccgagc acttgctgtt cttcccacaa tgcttccatc
    14341 attgaatact agtgtcctaa catcagctgg taaatctagt tcttcagcat gtgagattgt
    14401 taaatctgtg atgatcgttc ctggactccc ttttgctagt tgataagctg tcttcctatc
    14461 aaaaacttca tgaacattat taccgtaaaa tgtcgtttct acaatcgatc gaatactgga
    14521 taacagtgga tttccacttg tgatttcgtt taattcgaga ctctcttttg tactcatggc
    14581 tcctcctcat tccctaaaca aaaaaacgtt ttctttatta tacattattc gagtttttat
    14641 ttgtactgct gcgctgtggg aagcaaacga gttatttttc acacaaaaaa accgagaact
    14701 tgaataaaca agtttctcgg tttcatcatt ttccttttat cggcttagta ccagccgttt
    14761 gctaaccaga atgttttagc agcatcccat gaaccataac gagatgttac atattgttct
    14821 gctacacgtt cttgattggc agctgaatag tcgccgttta agtatgaagc atctaattga
    14881 taacggccga tgtaacgtcc gttagtagct gtgtatgaac cgcctgattc tttttgtgcg
    14941 atccattctt ttgcagagct tgaagttggc gctgttgcag cagctggggc agcaggagct
    15001 acttcaactg tttcaacagg tgcaacgtat gtatgttctt gcgttgctgt ttcagttgta
    15061 gatgctgtat tttctgttgt tttgttttct gttgttgctg aagaatcatt tgaagttggg
    15121 attgttaatt gttcaccaac ataaatgcgg ttgatgtttg cgatcgaatt tttttcagca
    15181 attgcatcga tcattgagtt gtctcctgca aatttttgtg agatcttaga taatgaatca
    15241 cctgatttta ctgtataaac ttcatcagcg tgagcatttg tagttcctac aaacattgca
    15301 gtagcaccag cagcaaaagt tgtacctaaa acaattttct taaatgaatt catattaaac
    15361 tccttttttt ctccgttttt tattctttcg acgctattaa ctttatcatt tagaaatatt
    15421 tcataggtaa aagaaacatg acat
//
LOCUS       DAZKNH010000059        15255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000059 DAZKNH010000000
VERSION     DAZKNH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.059"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..1021
                     /locus_tag="ACG8MG_001770"
     CDS             2..1021
                     /locus_tag="ACG8MG_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0375811.1"
                     /translation="MSIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDHQLIT
                     GKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGWLFFF
                     TSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVVIGMI
                     ISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSV
                     FLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSMTNIL
                     LFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTV
                     RRLKR"
     gene            1018..1785
                     /locus_tag="ACG8MG_001771"
     CDS             1018..1785
                     /locus_tag="ACG8MG_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0375812.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="ACG8MG_001772"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="ACG8MG_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFQ0375813.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="ACG8MG_001773"
     CDS             3750..4070
                     /locus_tag="ACG8MG_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFQ0375814.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="ACG8MG_001774"
     CDS             4070..4420
                     /locus_tag="ACG8MG_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFQ0375815.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /gene="hprK"
                     /locus_tag="ACG8MG_001775"
     CDS             4587..5525
                     /gene="hprK"
                     /locus_tag="ACG8MG_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFQ0375816.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /gene="lgt"
                     /locus_tag="ACG8MG_001776"
     CDS             5540..6370
                     /gene="lgt"
                     /locus_tag="ACG8MG_001776"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0375817.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="ACG8MG_001777"
     CDS             6414..7439
                     /locus_tag="ACG8MG_001777"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0375818.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="ACG8MG_001778"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="ACG8MG_001778"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0375819.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="ACG8MG_001779"
     CDS             8517..8957
                     /locus_tag="ACG8MG_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFQ0375820.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="ACG8MG_001780"
     CDS             8960..9562
                     /locus_tag="ACG8MG_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0375821.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9625..10728)
                     /locus_tag="ACG8MG_001781"
     CDS             complement(9625..10728)
                     /locus_tag="ACG8MG_001781"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFQ0375822.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG8MG_001782"
     CDS             10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG8MG_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFQ0375823.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12294..12689
                     /locus_tag="ACG8MG_001783"
     CDS             12294..12689
                     /locus_tag="ACG8MG_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375824.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12689..12940
                     /locus_tag="ACG8MG_001784"
     CDS             12689..12940
                     /locus_tag="ACG8MG_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375825.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12983..13378
                     /locus_tag="ACG8MG_001785"
     CDS             12983..13378
                     /locus_tag="ACG8MG_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375826.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13375..14631
                     /locus_tag="ACG8MG_001786"
     CDS             13375..14631
                     /locus_tag="ACG8MG_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFQ0375827.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14643..14966
                     /locus_tag="ACG8MG_001787"
     CDS             14643..14966
                     /locus_tag="ACG8MG_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375828.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14959..15225
                     /locus_tag="ACG8MG_001788"
     CDS             14959..15225
                     /locus_tag="ACG8MG_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375829.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5242 a         2580 c         3225 g         4208 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta attgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 aaggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcagg cgttagaggc agaaatcaaa
     2341 gagttagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaatctttct gtgtctttag taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aatagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatgatgag atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat tgatcgatga ctatgagcgt acacacgctt ctacgattgc
     7921 cgttatgcaa gttcctcacg atgaaacttc aaaatacgga atcatcaacc caggggaagt
     7981 actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct ttgatgtatt
     8101 atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa tcgatacgtt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg acgttggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag aagtaggggc
     8281 acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 caatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aatctttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     8581 tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     8641 aagcaaatac gacaattgag atcgtcacac aagacgtaga tcttttatct agacaagtag
     8701 aaggcttgct agtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     8821 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     8881 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     9001 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     9061 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     9121 ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc
     9181 tcaacaagca ggggatctag caggcaatgt taagcagaag gtaagtgaat cagcagagga
     9241 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     9301 taatcgtttc aaaaaaacag cagaagatct gaaaaagcca gcagacgaat taggtagtat
     9361 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     9421 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     9541 agattcgact accaatcact aagattactc atttctagaa taaaaaaggt gagagcggac
     9601 cagctctcac cttttttatt ttcattactc taaaacaagt aattcttttg gtgtagtcgt
     9661 gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta cccctacttt
     9721 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcaa tcactaagtc
     9781 gtttccttct acaagagaag ggaattcgtg gacggttgtt ccgattccgt gacctaaacg
     9841 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
     9901 ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt
     9961 gatgttatag attttttctt ggaactcagt tggtttttga taagcaactg tacgagtagc
    10021 atcactgcaa tagccattcc aaataacgcc aagatcgaat aacactagtt catttggtga
    10081 aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    10141 tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga tttctgcgat
    10201 aattgcttgt tcagttacgc cttcttttac tgttttgaag ccaatttcaa aggcaacatc
    10261 ggcccattta ccagcttcga tcagtttgtc tttttcttct accgttttga tcagttgcag
    10321 acgctggata atagaagtta gatcggtgta agaatctgta cctgtcaaga tatcagagat
    10381 agcatcgaag cgatcgacag tcagagcatt cttttcaata cctacattac caggttgccc
    10441 atagcgtgcg cgaagctctt ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc
    10501 acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag caggagtgaa
    10561 aaggaaagga tcttgttcca gagggataaa aagagctagt acacgctcgt gaggatcact
    10621 ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attctacttg
    10681 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    10741 tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag accaacgttc
    10801 ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattgttg
    10861 agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    10921 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    10981 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag
    11041 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg
    11101 cacgtggctt ggcaagtaag aaaacaacaa cagttggggt aatcattcct gatgtcagca
    11161 atatgttctt tgcctcttta gcacgtggta tcgacgatgt ggcaacaatg tacaaataca
    11221 atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta ttgaataatt
    11281 tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc
    11341 gtggagaatt ttcacgttca aaaacgcctg ttgtactagc tggttccatt gatccagatg
    11401 aacaagtagg tagcgtaaac attgattatg tgtcagcaac aaaagatgca gtaaataaat
    11461 tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca
    11521 atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat ctaccatata
    11581 gcgaaggatt gatttttgag gcacaataca actttaaaga tggtctgtct ctagttgacc
    11641 ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta gccattggta
    11701 tcttggatgg tttattagac cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa
    11761 gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc acacaaccat
    11821 tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat aaagaagaag
    11881 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca actaaataat
    11941 aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa cttctggaac aacgtttata
    12001 cgagaaaggg ggttatgaca aaattttgtc ataacccctt tttttttgtg actttaaaaa
    12061 aagagaaaat ggcggaaaca aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga
    12121 tgctacattt tgttcactac tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa
    12181 agtagaaaag tagataagta actctaaaaa atccaaaaaa atccaggctc gattgcgtac
    12241 ttatcttaaa ggagaaacaa tccatcatat ataacccgaa aggagacttc tatttgccta
    12301 agaaaagacg aaagaccata gtcatcattt tgaccattct tactatagga atcggaggga
    12361 aattttacat ggacaaaaga gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac
    12421 agtccgtcaa agttctgaaa aatacctttg cggatatcaa ggaagtgaag attgaaaaat
    12481 ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat tctaatgact aataaacaag
    12541 atcaatcgat ttatttttct tatagttttt ggaaagaaag aaatgagata ggaagttacg
    12601 gaatagttga tgaaaaaaag caaaaagaag gaaatacgct caataaagtt aaagtaactt
    12661 actctaatgg aaatgaggaa agtatttaat gattactgac aaacaatata gtgaattaag
    12721 tgatgctgtt tactggctag atcctaaaca tagagactat gtacctgaaa tgcaagaaaa
    12781 tttaagtttt aaaattaacg gcactatgta taaaatccta aaaatcaaaa actccttcga
    12841 cggcatgcag gcgatggctg tagcgccaat cgtccatagc aaattggaga aaaatttcaa
    12901 aaataaaaaa attcctgcaa actttcgcgt acttaaataa tgaaggtgtc aatacttaca
    12961 taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga aaactcagct tagccatttt
    13021 attaatcgtc atattaggga tcggagggaa agtctatatg gacaaaagag aagcacaaag
    13081 gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc
    13141 ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttaccg gaagttatgg
    13201 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt atagtttttg
    13261 gaaggagaga aatgaacttg gtggttttgg cattgagaac agagcggtac aaaagacagg
    13321 agtcactata aaaagagtaa aagtaatttt ttctaacgga caggaggagt tagtatgaca
    13381 ataaacgata agcattacaa tgatattagt gagaaggttt attggctaga tcccaaatat
    13441 ccccgctaca atgagggata caaaaagaac tctgttaaag aatttgctgg tatggaattt
    13501 caaatcctac aaatcaaaga ctcattggac ggcatgcagg cgatgtctgt agcgccaatc
    13561 aagaacggta aagtggatct ttctcaagta gtgattgctt ttgcaggaac gaacttaagt
    13621 gattggaaag atttggaaac ggatgcaaga agtatcatga atctctacgg gaatagacca
    13681 ggagcaagtt tttctgaaaa agcaacaaca aacaagattt tatccgttag tggattgaaa
    13741 acagcggggc aaatcaattc agcgattgca tttgctaatg aagtaaaaaa ggaatatgga
    13801 gaagctgcta tttctactac cggccattca ttaggtggat tcttagcatt atatgtagca
    13861 gcagagaaac aatggaaaaa tgtcggcttc aacgggccgg acccgtatga tctcttatca
    13921 ccagaagcaa aaaaatgggt ggaagaaaat cccggtatgc tcaccaatta ccgaaatcac
    13981 gccgatttta tcggtaattt tggtggtaat ggcacaggtg ctgaagttcg gatcagtatg
    14041 gaaacagcat tcttgaatct gcttgcttcc cacagtttaa cctcttggaa gtttgatgaa
    14101 aatggaaagc tgattattcc tgaaaatgaa aacaatgtta aagctcgaaa acaacagaaa
    14161 gaaaaccaag cgacccagtt gttttcctat cagttgcaag aactgaacaa catacgtaaa
    14221 aaactacgat taagtggcgg ccggctgaca gttaatgaac aaatttactt agatgacaca
    14281 caagctaggt tagtagtgga aaagttatcg gcaaaaatga aaagcgctat ggaagaagtt
    14341 atccgcatca atcaaagagg aatttttgag ctagagaaaa aatggcaaga aggcttgcgg
    14401 caagcacgtg ttgtagcccc ttcgttgact gaacaggaaa tcatagaagc attagctagt
    14461 gtgggcgcca caaaagaaaa cttagtcgat tctgcgaaag aagtctatct gctacgaatc
    14521 aataaggcaa gacgtatggg ggaggccttt gatacattgg cgaaggagat ccaagacaaa
    14581 atcaatgaaa tcgtggcacg agatcgagaa ttagcacagc aattcaatta aaggagaagt
    14641 agatgaaact agaagaattg gaaagagatt atcgaagtga acgaagacaa ttagaagctc
    14701 aagaagatga attgatccac tttaagaatc aaggattacg attgatggag caagaggagc
    14761 agcgactgca tcatgtatta aagaacttag ctttagataa tgagcctctg tttcaagcaa
    14821 gacgaagtta ttcgaattat caggagcatt atcaacaagc cctacaagta gaacgacgaa
    14881 aactctatca gcagcaagat gaactagaac gttcctatcg tcaaaaaatc agacaaataa
    14941 ctatggagaa atcatcttat gaatgaattt ttaatgcaat tactaaaaga attagagaaa
    15001 aatctacatc gatcaaatgt acgttcagtt ggtatgggga tatctcagca aatttgggca
    15061 gaaaaacgat tgacagatca aaactaccaa atgactgcag aagaagctgc tcaagaattt
    15121 tctaaccgac atgtacactt gaaaggatca gtaaaagggc aatttgcaga aaaactaact
    15181 gaattattcc aaagacaaga aaagaaatta aaacagtttc gttagtaaag ttatatagaa
    15241 aaattttgtg aaaat
//
LOCUS       DAZKNH010000060        14220 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000060 DAZKNH010000000
VERSION     DAZKNH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.060"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACG8MG_001789"
     CDS             complement(<1..1835)
                     /locus_tag="ACG8MG_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0375830.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACG8MG_001790"
     CDS             complement(1832..2536)
                     /locus_tag="ACG8MG_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0375831.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACG8MG_001791"
     CDS             complement(2548..4857)
                     /locus_tag="ACG8MG_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0375832.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACG8MG_001792"
     CDS             complement(5052..5516)
                     /locus_tag="ACG8MG_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375833.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACG8MG_001793"
     CDS             complement(5516..6142)
                     /locus_tag="ACG8MG_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0375834.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACG8MG_001794"
     CDS             complement(6149..6514)
                     /locus_tag="ACG8MG_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0375835.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACG8MG_001795"
     CDS             complement(6504..6842)
                     /locus_tag="ACG8MG_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375836.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACG8MG_001796"
     CDS             complement(6832..7158)
                     /locus_tag="ACG8MG_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0375837.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="ACG8MG_001797"
     CDS             complement(7136..7417)
                     /locus_tag="ACG8MG_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0375838.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="ACG8MG_001798"
     CDS             complement(7419..8783)
                     /locus_tag="ACG8MG_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0375839.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACG8MG_001799"
     CDS             complement(8796..9365)
                     /locus_tag="ACG8MG_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFQ0375840.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACG8MG_001800"
     CDS             complement(9337..10524)
                     /locus_tag="ACG8MG_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0375841.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACG8MG_001801"
     CDS             complement(10588..12315)
                     /locus_tag="ACG8MG_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFQ0375842.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACG8MG_001802"
     CDS             complement(12312..12764)
                     /locus_tag="ACG8MG_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFQ0375843.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACG8MG_001803"
     CDS             complement(12876..13256)
                     /locus_tag="ACG8MG_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFQ0375844.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACG8MG_001804"
     CDS             complement(13253..13639)
                     /locus_tag="ACG8MG_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0375845.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACG8MG_001805"
     CDS             complement(13641..13919)
                     /locus_tag="ACG8MG_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375846.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13982..>14220
                     /locus_tag="ACG8MG_001806"
     CDS             13982..>14220
                     /locus_tag="ACG8MG_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375847.1"
                     /translation="MLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGVSSYSA
                     FDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEG"
BASE COUNT         3937 a         3057 c         2240 g         4986 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
//
LOCUS       DAZKNH010000061        14152 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000061 DAZKNH010000000
VERSION     DAZKNH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.061"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..3686)
                     /gene="rpoC"
                     /locus_tag="ACG8MG_001807"
     CDS             complement(33..3686)
                     /gene="rpoC"
                     /locus_tag="ACG8MG_001807"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFQ0375848.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3765..7382)
                     /gene="rpoB"
                     /locus_tag="ACG8MG_001808"
     CDS             complement(3765..7382)
                     /gene="rpoB"
                     /locus_tag="ACG8MG_001808"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0375849.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8110..9078
                     /locus_tag="ACG8MG_001809"
     CDS             8110..9078
                     /locus_tag="ACG8MG_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0375850.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9096..10001
                     /locus_tag="ACG8MG_001810"
     CDS             9096..10001
                     /locus_tag="ACG8MG_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0375851.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9998..10804
                     /locus_tag="ACG8MG_001811"
     CDS             9998..10804
                     /locus_tag="ACG8MG_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375852.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10961..11929
                     /locus_tag="ACG8MG_001812"
     CDS             10961..11929
                     /locus_tag="ACG8MG_001812"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFQ0375853.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12246..12770)
                     /locus_tag="ACG8MG_001813"
     CDS             complement(12246..12770)
                     /locus_tag="ACG8MG_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFQ0375854.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13057..>14152
                     /gene="scm"
                     /locus_tag="ACG8MG_001814"
     CDS             13057..>14152
                     /gene="scm"
                     /locus_tag="ACG8MG_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0375855.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTS"
BASE COUNT         4006 a         3054 c         2792 g         4300 t
ORIGIN      
        1 aataatttgt cacaacctct tttttaaaat atctattttg tttgattgtt cattgcgtct
       61 tctgcagcca tttgcgcttc aatatctgaa atactgtaga cattttcgct tgctacaccg
      121 acttcttttg gttccatgtc acgataacgt tgcataccag taccagctgg gatgatctta
      181 cctataataa cattttcttt caaaccaaga agaggatctt tcttaccgcg gatcgcagca
      241 tccgtcaaga cacgagttgt ttcttggaat gatgcagcag ataagaaact atttgtttct
      301 aatgatgctt ttgtgatacc tagtaagaca ggtcgagaag ttgcaggaac gcctcctgag
      361 actagcgtat tgtagttttg atctttaaag tctgcgatgt ctaataatgt acctggtaag
      421 atatctgtat cacctggatc cattacacga actttacgca gcatttgacg aaccattact
      481 tcgatatgct tgtcgccgat ttctacccct tgcatacggt aaacacgttg tacttcacga
      541 agcaagtagt tttcaacaga taatacatcg cgaacttgca acaattgttt tggatcgatt
      601 gatccttctg tcaatggtgc accacggtgg atcatatcgc cttctgctac tttcatacgt
      661 gctgtataag gaacagtata cgtacgtgta tctgtttttc ctttgattgt tacttctttg
      721 gtacgtgttg caggatcttc tgagatatcg atgacatccc ctgttacttc tgtgatcact
      781 gcttgccctt tcggattacg tgcttcaaag atttcttgga cacgaggaag accttgagtg
      841 atatcgtctc cggcaacccc acctgtatgg aatgtacgca tcgtcaactg agtaccaggt
      901 tcaccgattg attgagcggc gatcgtacca actgcttctc cgacttcaac gtcagagcca
      961 gttgcaaggt tgcgtccgta acaatgtttg cagacaccat gttttgtatt acatgtgaag
     1021 acagaacgga ttgtcactgt ttcgacacct gcatcaacga tttgttttgc gatgtcttcg
     1081 gaaatcaatt ggtttcctgc aatgatcaat tcatttgttt caggatgacg tacttcttta
     1141 cgggtgtaac gtcccactaa acgatcttct aatgattcga tgatttcatt tccctcgcgg
     1201 atggcttgga tttcaagacc tcggtcagta ccgcaatctt cttcacggat gattacatct
     1261 tgggccacgt caaccagacg acgagtcaag taacctgaat cggcagtctt caaggctgta
     1321 tcggtcatcc ctttacgagc cccgtgagtt gagataaaca tttccaagac ggatagtcct
     1381 tcacggaagt tcgaaatgat tggcaattcc atgattcgac cattaggagc ggccatcaaa
     1441 ccacgcattc cggcaagctg tgtaaagttg gaaatgttac cacgagctcc agaatcagac
     1501 atcatgaaga ttggattttt cgcttccaaa ccttccatca gtttttgttg gatgctgtct
     1561 tttgcagcat tccaaacagc aatgacacgt tcataacgtt cgtcatctgt gatcagacca
     1621 cgacggaatt gttttgtaat agtttcaact tgtttatggg cattttcaat gatttcttgt
     1681 ttttcattca gtacactgat gtcagcgatc cctactgtga taccggcatg tgtcgaatgt
     1741 ttgtacccta agtctttcat acggtcaagc atcttagaag tttctgttac tttgaatcgt
     1801 ttgaagactt ctgcgatgat gtttcctaag tttttcttct tgaacggtaa tactaattct
     1861 tgctctttga tatgtgcagg aatatcagta ccagcttcaa caaagtattt gtccggtgtt
     1921 tggacggtca agttgaagtc tgttggttca ttcagataag ggaattcagg aggcatgatc
     1981 tcgttgaaga tgatcttacc aactgttgtg atcatcgtac gttctttttg ccattcagta
     2041 aatggttttt cacctaatga gttagggttc acaccgatac gtgaatgtaa atgaacatat
     2101 ccgttgcgcc atgcgataac ggcttcgttt tgatctctga agaccattcc ttcgccttcg
     2161 cgtccttctt cttccattgt caagtagtag ttacctagaa ccatatcttg ggaaggagta
     2221 acaactggtt taccatcttt agggttcaag atgttttgag cagctaacat caacatacgt
     2281 gcttctgctt gtgcttcttc attcaatgga acgtggacag ccatttggtc tccatcgaaa
     2341 tcggcattat aagcttcaca tacaagtgga tgcaaacgga ttgcgcgtcc ttgtactaaa
     2401 actggttcga atgcttggat accaagtcgg tgaagcgtag gtgcccggtt cagcaatact
     2461 gggtgttcct tgattacgtc ttctaaaaca tcccatactt cgtcttcttg acgttcgatt
     2521 ttacgtttcg catttttgat gtttgaagct agttcgcgtt gaacaagctc acgcatcacg
     2581 aatggtttga ataattcaat ggccatttct tttggaagtc cacattggta catcttcaag
     2641 aaaggtccaa caacgataac ggaacgacct gagtagtcga cacgtttacc tagcaagttt
     2701 tgacggaaac gaccttgttt ccctttcaac atatgagaaa gagatttcaa tgggcggtta
     2761 cctggtccag taactggacg tccgcgacga ccgttatcga tcaatgcatc tactgcttct
     2821 tgcagcatac gtttttcatt ttgaacgatg attccaggtg cattcaagtc taacaaacgt
     2881 ttcaaacggt tattacggtt gattacacga cggtaaagat cattcaagtc acttgttgca
     2941 aaacgtccac cttctaattg aaccattggg cgtaaatctg gtggaataac agggataacg
     3001 tccataacca tccagcttgg ttgatttcct gaagcgcgga atgcttctaa aatatccaaa
     3061 cggcggattg cacgtgtacg tttttgtcct tgtgcagatt tcaattcttc tttcaattcg
     3121 gctgcttctg catctagatc aacattatca agtaattgtt taatcgcttc tgcacccata
     3181 gcagcttgga attcttgtcc atattgttca cgtttttcgc gatattcgcg ttctgtcaac
     3241 aattgttttt tctctaaatt cgtatttcct ggttcgatga ctacatatga agcaaaataa
     3301 atgatttctt ccaaagcgcg tgggctcatg tctaatacaa gacccatacg tgaaggaatc
     3361 cctttaaagt accagatatg tgaaacagga gctgctaatt caatatgtcc catgcgttca
     3421 cggcgaactt tcgaacgagt aacttccact ccgcatcgat cacaaacgat tcctttataa
     3481 cggatacgtt tgtattttcc gcaggcacat tcccagtctt ttgtaggacc aaaaatccgc
     3541 tcacagaaca acccttcgcg ttcaggtttt aaggtacgat agttaatcgt ctcaggtttt
     3601 tttacttcac cgtaagacca gcttctgatt ttttcgggag aagccaaacc tatttgcata
     3661 ctttcgaatt tatttacatc gatcaaaagg ggttccctcc atttcattta atggcaaacc
     3721 gcaagacggc tgatcttcga ccgccttgca gctcatagac cggattagtc taaattatct
     3781 tctgcggttt caaagttttg gatgacatcg tctttttcat cttccacttg ttcagcagct
     3841 ttcttttcta attctttggc agtttgctgt tcggcaaatt ttgtcaaagc atcaacagtg
     3901 atcaaatcgt cgtcttcatc gtccatgtcg cgaagttcga tctctgtttc ttcgatatcc
     3961 aagacacgca tgtccagacc tagtgattgc aattctttca ccaatacgcg gaatgattcc
     4021 ggtacacctg gttttggaat tggttcaccc ttgacgattg cttcgtatgt tttcacacgt
     4081 ccgactacgt catctgattt gtatgtcaag atttcttgta aagtataagc cgcaccgtaa
     4141 gcttccagtg cccatacttc catttcacca aaacgttgtc caccaaattg agctttacct
     4201 cccaatggtt gttgtgtaac caatgagtaa ggtccgattg aacgtgcatg caatttgtca
     4261 tcgaccatgt gagcaagttt gatcatgtac atcacaccga cagacacacg tccatcaaat
     4321 ggttcacctg tacgtccgtc gtacagaaca gttttcgcat cgctggccat accggcttca
     4381 cgaactgttt cccatacgtc ttcatcactt gcaccatcaa atactggcgt tgctacgtgg
     4441 atacctaact gacgtgcagc catacccaag tgtaattcca atacttgccc gatgttcata
     4501 cgtgaaggca cccctaaagg attcagcatg atatcgattg gtgttccatc aggtaggaat
     4561 ggcatatctt cttccggcat aatacgggat acaacccctt tatttccgtg acgaccggcc
     4621 attttatccc cttcatggat tttacgtttt tgaacgatat aaacacgaac taacatgttt
     4681 acgcctggtg acaattcatc tccagcttca cgagtaaaga ttttcacatc gtgaacgata
     4741 ccgccgccgc catgtggtac acgtaatgat gtatcgcgga cttcacgagc tttttcgcca
     4801 aagatcgcat gcaatagacg ttcttctgca gataattctg ttaccccttt tggcgtgact
     4861 ttccctacaa gcaaatcgcc atcttggact tctgcaccaa tacggatgat acccatttcg
     4921 tctaaatctt ttaatgcgtc ttccccgaca ttagggattt cgcgtgtgat ttcttcaggt
     4981 cctaattttg tatcgcgggc ttctgattca tattcttcaa tatggataga agtgtatacg
     5041 tcatctttta ccaaacgacg gctcatgatg atcgcatctt catagttgta cccttcccaa
     5101 gtcatgaaac caactaaaac gttttggcct agagccattt cgccttgttc catagatggt
     5161 ccgtctgcca gagtatcccc tttttcgact ttctcaccca aatgaacgat tggacgttgg
     5221 ttgtagcttg ttccggagtt tgaacgacgg aattttgtta catcgtattt atctaatgca
     5281 ccgttgtcgc ggcgtacgcg gatttcagaa gcatcaacgt attcaacgac accgtcatgt
     5341 ttacataaca aagcagctcc tgagtcatgc gctgatttgt attccatacc agtaccaacc
     5401 catggtgctt gaggattgat caacggcact gcttgacgct gcatgttggc acccatcaag
     5461 gcacggttgg agtcatcgtt ttccaagaaa ggaatacatg ctgtcgcaac ggcaactact
     5521 tgtttaggtg atacgtccat gtagtctact ttatcaatag atacttctaa gttttcactt
     5581 tgagcacgag ccataacaac gtcttgagca aatgttccat cttcattcaa tgggctgttt
     5641 gcttgggcta cgatgtaatg gtcttcgata tcagctgtca agtagtcgat ttggtcagta
     5701 acacgacctg tttcacggtc tacacgacga taaggtgttt cgataaatcc gaatttattt
     5761 actttcgcat aactggacaa actattgatc aacccgatgt ttgggccttc aggagtttcg
     5821 atcggacaca tacggccata gtgggagtag tgaacgtcac gaacttcata accggcgcgg
     5881 tcacgagtca atccgccagg tcctaaggct gaaagacgac gtttgtgtgt caattcgcca
     5941 agagggtttg tttgatccat gaactgtgac aactgagaag aaccaaagaa ttctttgata
     6001 cttgccacaa ctggacggat attgatcaat tgttgaggtg tcaatgtttc agtatcttgg
     6061 atcgacattc tttcacgaac cacacgttcc atacgtgcta gcccgatacg gaattggttt
     6121 tgtaaaagtt caccgactga acggatacga cgatttccta agtggtcgat atcatcgaca
     6181 ttgccgatac cttccatcaa gttcaagaaa tagctcattg aagcaatgat gtcggctgga
     6241 cgaaccgttt ttacagctgc ttctggatag ccgtttccga tcacgtttac ttcacgttca
     6301 gggtctcttg gtgaaaatac tttgatgact tggacagtca ttggatctgt gaccacaccg
     6361 tcttctgatg gatagtaagt cactgaattt aggccattat caatgaaagg agctagtgtt
     6421 tccatgactt ggtgagtcag aactgttcct ttttcaacga tgatctcgcc tgtttctgga
     6481 tcaactaatg tttcagctaa tgtcagattc aatagacgtg ttttcaaatc taattttttg
     6541 tttactttgt aacgaccaac gtttgctaag tcgtaacgtt ttggatcgaa gaaacgagca
     6601 ttcagcaagt tacgtgagct gtctgctgtt tttggttcgc ctggacgcaa acgttcgtaa
     6661 acgtctttca aaccttcttc tgttcttgag tcgcttgcgt ttttatgcaa gtctttttcg
     6721 actgtattgc gtaatgtttc gctgtcgccg aagatttcaa agatcgtatc atcggaacca
     6781 aatcctaacg cacgaactaa tacggttaaa ggaatcttac gtgtacggtc gatacgtaca
     6841 taagagatgt cttttgcatc agtttccatt tctagccaag ctccacggtt agggataaca
     6901 gtagaaccga atccttcttt tccgtttttg tcgacttttc catggaaata gacacctggt
     6961 gaacggacta actgagaaac gattactcgc tctgccccgt tgatgatgaa tgttccttgt
     7021 tctgtcatta atgggaaatc accgaagaat acttcttgtg ctttgatttc gccagtctcg
     7081 cggtttgtca aacgcaatgt tacatgtaat ggtgcagagt agtttgcatc atgcgcacga
     7141 gcttctgcta ctgtatactt tggttctttc aattcataat ctacaaattc caaagataga
     7201 ttgccattga agtcatcgat tggcaaaatg tcttcaaaca tttctctaag tccctcatct
     7261 aagaaccatt ggtaagagtc tgtttgaatt tcgatcaaat ttggtaattc caacacttca
     7321 ctgattcttg cgaaacttct acgttcgcga tgttttccgt attttactac gtgtccagcc
     7381 aagctcttca cccctctaaa atgtcattca aaaagactac ccgatcaatt gttacaataa
     7441 tatttcaaat attattttta ggtaacaaca gacatttttt gcggctttta ggcaaaaaaa
     7501 aaccaaaaat agaacattct cttgaaacct tacttcgtga gcttctactt ttgtttcctt
     7561 atattaaagc cgaaacggac aatatttcgt ttctgccttt tcttcatcag atagtttttg
     7621 caacttcata tactacactt attttctata aaagtcaaca cttcctggcg tttcttctgc
     7681 tttttcgcaa cattttctta atgttttcaa ttttatgttt tcaattatct gacaattctt
     7741 aattgacaga cctttttatc tcggttatac tttagaaaat tcattttgtg gaggaacttc
     7801 tatgcgaacc aacatcaacc aaatcgcacc aacacaactg aataactttt actttagcta
     7861 ctttagatag gtttgtattc atcatgatgg atacaaacgt taaacgacag tttgtatcta
     7921 tgatggctaa agaactttgt attgcagcaa aaagccacgt agacaaaatg ctaagtggct
     7981 gagcagtgaa aatacaaagt aaaagtatca agcattctac tttttcaatt tattttccga
     8041 cttttcaatc catttggaca ttttgtctga atggattttt tgtttcccaa aattaaggag
     8101 gaaaaagaaa tgaaaaaaaa tataatcatc ggattaggcg ctgccctgct actagcttta
     8161 ggaggttgcg cacaaaaaga caattccgca gaggcatcag atggacaaaa aacaattggc
     8221 gtattgcaac ttgtacaaca taactcgtta gatgctgcct ataaaggatt caaagaagga
     8281 ttagcagaag aagggtataa agaaggcgat aatctaacaa tcgattatca aaatgcccaa
     8341 aacaatcaag acaacctgaa aagtatgagt gagaaactcg tcaaagaaga tcctgatttg
     8401 ctacttggaa tcgccactcc agcagcacaa tctttattaa atgaaacaac cactatcccg
     8461 atgactgtca cagcggtcac agacttggaa caagcccatt tagtcacatc caatgaaaaa
     8521 cctggcggca atgtgactgg cacgacagat atggtgccaa tcgaaaaaca aatcggctta
     8581 ctgttaaata tcgtgccaga cgccaaaaca atcggcatca tgtataacaa cggtgaagca
     8641 aactctaaga tccaagctga tttggcagaa aaagaattga aaaaagcggg cgtcaaagtc
     8701 aaagtactga cagcaaattc cacaaatgat gtccaacaag tgacgaaaag tctagctaaa
     8761 gatgtagatg gaatctatat tcctactgat aacacgttcg caagtgcggc aagtgttgtc
     8821 ggtgaagtcg caaaagaaac aaaaacacct gtcgttgctg gcgcagttga tcaagtcaaa
     8881 gacggcggtc tagcaacagt aggaatcgac tacgaaagct tgggtaaaca aacagggatc
     8941 atggcagcaa aaatcttaga cggcaagaat ccaagtgaac ttcctgtcga aacagcgaat
     9001 gatttggaac tgtatgtcaa taaagacatg gccaaagcat taggcatcaa tccagacagc
     9061 atcaaagaag ctgaataaaa cacaggagga ttctcatgac aaatttgctc tctcttttct
     9121 tatcatctgc atcgcaaggg gtcttatggg ccctacttgc gattggtgta tacgtcactt
     9181 tccgtatctt ggacattgcc gacttgactg ctgaaggaag ctttccttta ggtgcaggga
     9241 tcgcggcagt aagtattaca aatggctatc atccattgac agcttgtctt ctaggctttt
     9301 tagggggtgc ggcagcaggg cttgtttccg gactgcttca cacaaaattg aaaattcccg
     9361 cccttcttgc agggatcatc accatgactg gtttatattc tgtcacatcc cgtgtgatgg
     9421 gtgcgccgaa catctcttta ttgggtcagc caacgatctt tcattgggtc gagtcattcg
     9481 gcataacaaa agacaacgct gtactgatcg tcggtctgct ttttgctttg atcgttgtat
     9541 gtttacttgt tttgttctta cgtacagaaa caggacttgc tattcgtgca actggcgata
     9601 atctggcaat gagcgaagca aacgggatca acacagataa tatgaagatc atcggttata
     9661 tgatttctaa tggatgcatt gctctttctg gctccttgct tgcgcaaaac aacggctttg
     9721 ctgatttaaa ttctggtatt ggaacgatcg taattggact tgcttctatt atcatcgcag
     9781 aagttttgtt acgtaaccag ccactatttc tccgtctctt gactgtcgtt ttaggtgcgg
     9841 tcatttatcg ttttatttta gcgttagtct tcgaactgaa tgttgaacca agtgattcga
     9901 aacttgcttc tgctttcgtg ctagtgatct gtctttcttt cccgcagatc cagcaaaaat
     9961 tccatttacc tcaaaaaaag aaaggcggta gtgtcaaatg aatgacgtct taacattgga
    10021 aaatctccat catacctttg aaaaaggaac gatcaacgaa aatcatgtcc tgaaaggcat
    10081 taatctttcc ttagatcccg gcgacttcgt gacgatcatc ggtggaaatg gggctggtaa
    10141 atctactttg ctgaacagca tcgccggtac attttctgtc gatcagggtc aaattttatt
    10201 gaatggaaaa aatatcacga aaaaatctgt tgtcgaacgt tcgaaatcca tcagtcgcgt
    10261 attccaagat cctaaactag ggacagccgt tcgtttaacg gtagaagaaa atcttgctct
    10321 tgctatgaaa cgaggaaaaa aacgcggctt cttccgtggt gtcaaaccac aagaccgaac
    10381 atttttcaaa gaccagctgg ttcggctaaa tttggggttg gaaaatcgtt tatcctcaga
    10441 aatcggtttg ctgtctggag gacaacgtca agctattacg ttattgatgg ccacattaca
    10501 gcgtccagaa ctgatcttat tagatgaaca cactgcagcg cttgaccctg ctacttccaa
    10561 aacggtgatg gaattgacag aaaagctgat cactgagcag aatctcactg cttttatggt
    10621 cactcatgac atggaagacg cgatccgcta tgggagtcga ctgatcatgc tgcatcaagg
    10681 aaggatcgtc gtagatatcc agcaagagga aaaacagcag ctcacagtgc cccaattatt
    10741 agcgttgttc aaacaaaaca gcggcacggc attaaaagac gatcaagtac ttttatcttc
    10801 ataatcagaa agaggatggg acaaaaaatg aacgcccacc ctcttctttt ttctctttcc
    10861 ttctttcgtt tttgctttat gtatcaaccg ttgtttcttc gaaaactatt ttgttctctc
    10921 cttcttcctg atagaatgga tgggaaagga gcttgatgcc atgacaacga aagacaaggt
    10981 acttcaatta ttgaaaaaga caccagattt tctttccgga gaaaagttgg cacagcaact
    11041 ggaagtttcc cggacaagta tttggaaagc catcaaagaa ttggaaaagg caggctatcg
    11101 ttttgagcac gggcctacag ggtatcgtta cctgccctct gacgtactag atgcaaaaga
    11161 aatccagcaa gagctacggt cgtttattcc tgatttgaaa gtacaaacga tgttgacttc
    11221 tgaatctacg atgaaagatg tcaaactggc agcagctcaa ggagattctg tttcgaagtt
    11281 atttgttgct gatacacaag aacaagctcg tggacgtttt ggacggcctt ttttcgctga
    11341 acctggacga ggaatctaca tgagtctctt gctccatcca gacaaacggt ttgatgaatt
    11401 gcctcaatat acgatcatcg cagccagtgc ctgtgtgaaa gccattgaat ctgtaaccgg
    11461 tcgttctgct tcgatcaaat gggtcaatga tatctacatg gatggaaaaa aagtctgtgg
    11521 gattttatct gaagcagtca gtgatatgga aagcggcagg atcagtcata tcatcattgg
    11581 gatcgggctt aatttttctg ttcctcaaaa tcattttcca gctgatctcc aagaaaaagc
    11641 catctctgtc tttcccgacg gacaagctag tgtcacaagg aatcagctaa tcagcaaaat
    11701 ctggcaagaa ttgttcactt atctttctca cccagaaaaa gcgttggact tctataaaga
    11761 aaaatcattc gtattgggaa aaaccgtgtc gttcaatcaa aaaggaacga cttatgtcgg
    11821 tcttgcaaaa gaaatcggtg atcatggcga actgatcgtc caaacaaatg aacagaagat
    11881 gagtttgtct tctggcgaaa tcagtctcac atctatccgt acgcaataat tatcagattt
    11941 cacaaaagga cacctgatct catacagtgt ccttttttct gaattctctt caaaagactc
    12001 tccttattct atcagctgac agtttctcta tcctctagaa aaaggtattc taaaaatatc
    12061 tataaagcat ggtacacttt ctttaaatga atcaatgcaa aaaacaagag agtcatcagt
    12121 ttacacgatc aaaagagata gcaggccgct tcctttttca atcttataga tagaccatgg
    12181 gtaacgataa aaacaaaaaa gaacttgcga cttattttgc cgtaagttct tttttagcta
    12241 gtgccttaat ctcttaatcc caacaaacgt ctcgcataga tcacctgacc agagtgcata
    12301 gcagcatcat caataataga aacgagacgc attcctcgtg tgactggtgg tgtccagttc
    12361 tcgtcaatta cttcatctag ctcggaaggt tccagccttc ttaagtaaga aatagcaaaa
    12421 tcagcagctc ctcgcaagta tcctagcaga tcttctattt gattagcttg gatctgcttt
    12481 gcttgttcca gactatgctt ccaatcttgt tcgttatctg ctatcgtcat cggaaaccgg
    12541 ctttcccagt cttgactatg ccaaattgga gaagttcctg ctaaatcagc aatctggaaa
    12601 tctaattctc gagcggtatg ccaagccagc caagtcattg atttgatctg cggggctcta
    12661 tcttccactg gaaaacgatt ggcctcctca atagagacgc cttcaaacaa acgttcaaac
    12721 cgatcgtatg cccgatccac actctcgcat agcatatcta aataatccat ttattttcct
    12781 cctttttatt tatcctactt actttttcct aattctagga tacctactta tatgttacta
    12841 cacaccctta ttttttacac tttaacagtc accatcatca atagtttgga gattgaaaga
    12901 aaaagatagt gtcgaactat caattgaaca agaagcaaca atatgaacgt ttcattaata
    12961 ttctttaaat aaacaatgat ttttcaaacg aacagtttaa gactaggaga aaaagagtat
    13021 aataggaaaa tgtttgaaag gaaaggaagg ggaaaaatga aaaaaagggc aagtttacta
    13081 gtcctagttg ctttgttgtt caatatcttt tcgtatggtg tggccgtttc agcggaaaca
    13141 tacattccta aagaccctgg gaatatttat aatgatctaa ctggtaacta tggcttgtta
    13201 ggaattgcta gtcaattcca cgtatttgct aaaaataaga caactgtgaa tgcacataca
    13261 gacggaaata tagcaacaca tgaacttgat gcaaaaaata attttggtac agagcttcat
    13321 gaaggtttat tgaaccaaga aataaattat atacaaaaaa ctgattcttt cgctaattct
    13381 tcaggcatac ctacaggtag cacaagaatg aataaatttg tcgttggcag taacgtggat
    13441 gtagcacttc aagatggtca tcctacagtc aatggatcca ggcttgatca cttaactcca
    13501 gatgattttt ataaagatcg tgctgggcat gaatatattg atttcgagca ggaatttgct
    13561 aaattgaatg tagcagcaaa tgatcttgca acgatcactc cagctaaaac atatactgct
    13621 gcagattttc cagacatgaa caatcggaca atcgatttaa ctgatttatc agataccggg
    13681 tttttattgg taaatatcga tgcagaagtg ttaacgatga atacgccact tcaaatcatt
    13741 aatccaaatg atcaagtagt tgtatttaat gtcatcaaca gttcttctgc attgaacgta
    13801 cagtcaccaa tcaaatacaa tgatcgttca aatcatgaaa cagaagattt ttcggatgcg
    13861 aatatctctt ggaattttgg aaatgagatg actgatttga caatcagtgc tccttttcaa
    13921 ggaacaatcc ttgcgcctaa tgctactatc cgcattactc aaaaccaaga cggaacgatt
    13981 ataggaacaa atgtgatttt agatgcagct accaatcgtt gggatcctaa cgaaatattt
    14041 atcagcgaca atggtacaga tacgacagac acaagcgaca gtacagatac atctaccaca
    14101 agtgatagct cagattcatc aacttcaagc gacagtacag atactacctc ta
//
LOCUS       DAZKNH010000062        14084 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000062 DAZKNH010000000
VERSION     DAZKNH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.062"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(125..1393)
                     /locus_tag="ACG8MG_001815"
     CDS             complement(125..1393)
                     /locus_tag="ACG8MG_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0375856.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(1624..2361)
                     /locus_tag="ACG8MG_001816"
     CDS             complement(1624..2361)
                     /locus_tag="ACG8MG_001816"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0375857.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(2428..2997)
                     /locus_tag="ACG8MG_001817"
     CDS             complement(2428..2997)
                     /locus_tag="ACG8MG_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0375858.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            3125..3997
                     /locus_tag="ACG8MG_001818"
     CDS             3125..3997
                     /locus_tag="ACG8MG_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0375859.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4339..5211
                     /locus_tag="ACG8MG_001819"
     CDS             4339..5211
                     /locus_tag="ACG8MG_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0375860.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            5208..7166
                     /gene="nagE"
                     /locus_tag="ACG8MG_001820"
     CDS             5208..7166
                     /gene="nagE"
                     /locus_tag="ACG8MG_001820"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0375861.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(7498..8349)
                     /locus_tag="ACG8MG_001821"
     CDS             complement(7498..8349)
                     /locus_tag="ACG8MG_001821"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0375862.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            8472..8939
                     /locus_tag="ACG8MG_001822"
     CDS             8472..8939
                     /locus_tag="ACG8MG_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFQ0375863.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(9202..9684)
                     /locus_tag="ACG8MG_001823"
     CDS             complement(9202..9684)
                     /locus_tag="ACG8MG_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0375864.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(9784..10647)
                     /locus_tag="ACG8MG_001824"
     CDS             complement(9784..10647)
                     /locus_tag="ACG8MG_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0375865.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            11245..12120
                     /locus_tag="ACG8MG_001825"
     CDS             11245..12120
                     /locus_tag="ACG8MG_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFQ0375866.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(12265..12417)
                     /locus_tag="ACG8MG_001826"
                     /pseudo
     CDS             complement(12265..12417)
                     /locus_tag="ACG8MG_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(12685..14016)
                     /locus_tag="ACG8MG_001827"
     CDS             complement(12685..14016)
                     /locus_tag="ACG8MG_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0375867.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         4289 a         2813 c         2495 g         4487 t
ORIGIN      
        1 agtaaacggt gttcaaaaac ttgcatctgc ggtaataagt tcaaccaaac aaaaaacatg
       61 aaaatctgtt tttgtttggc tgttcttact acttgatgca gggcagcttt ttcactacct
      121 ctttttaata accataaaat cctattcctt catatctcca gcccatcggt gttagccaat
      181 ctctttcctt agcactcaag gtatagtgat gggtaccagt tttttcattt ggattaaata
      241 gtcgataaat tggcttggaa ccaccagaat aaaaacttag accttcataa cgccaaccga
      301 tttttgttaa agagttgatc tcagattgtg ccattgtgta atgatggtct ccactgtaag
      361 gattgtataa acggtagaca ggttgtccgc tgtttggtgc ttgccaagca atcccttcat
      421 attcccagcc tacagttggc agataatttt tttctccggc attcaatgta tataaatgtt
      481 ctcctgtatg acggttatac agacgataca tcggagaagt tgaagttggt gctgtatcat
      541 actgtgtcag attataggtt tcgattacat tgttcaatgt agaaccataa ttaggcgcag
      601 tggcataacg acctgttagc caagccgtag catctttgta agagttcgta ttgcttttcc
      661 atgcaccgga atagtagtaa acgcctggct gaaaagaagt cgtcttcaat accgttacgt
      721 tatcttgcaa tgattctttg tacgaaggat attttctaaa agcggcattc accgtaatcc
      781 attgcccatt cacatattcc gaagtaggca tcgtaacaga ttggccatta tagcttcctt
      841 taatcccaaa tagattgtaa ttaggggcag aagccaaagt actgttcccc cagccacttt
      901 caacgatggc ttgagcaatc atgactgatg catataagtc attggcactt gccaaacttt
      961 gtgcagttga ggcaatacta tcaatgaacg cttgttgaga attagcagat gagcgagcat
     1021 aaaaagcata gccagtatag ttttcgaatt cagacaaatc tgcttgatgg gtatgtgtca
     1081 atgtttgccc atctgtcaat tggttcgtga tttcttctgc attctctgga ttgttctgag
     1141 cttctgctga tgtactgtct tcggtttgtg tttgttctgt tgtttcagtt gttggttctt
     1201 cggttgaaat tgtattttct gagacagtag ctgactgcaa agtactttct gtatcgtttt
     1261 caattcctgt ttctgaagaa ctcgatgtct gagttgcgac agtttctgtc gattcttcag
     1321 ctagcactgt tgtgttggca actgctagat ttcctaaaag aataacgcta aataataaat
     1381 tctttgcttt catctttttt ctcctttgaa ttatgaaact tttcctaaat aacagtgtaa
     1441 ctaaaaaagt aaaagcaagt caatgtccat atttaaataa catttgtttc tttttaataa
     1501 atttgctttt tctgcataaa aagacgtaaa tgaagaatat tcttctccat tcacgcctta
     1561 aattgatggt cgtttgaata actatttaac aagtaattct ctctggatat tatccgttat
     1621 ggattatact tcctgtgtcg tcggacggat aatcatgttg ctgacagaca tccggtctgg
     1681 cgtttctatc gcaaatgcta cagcagaagc tatatcttca gatgttaatc cccattcttt
     1741 ttgcgcttca tgaagttcaa gagcagcctt tttgtcgctg atggtttgat ataattcagt
     1801 ttgaacagct cctggagaga tgatcgtaga tttgatgtta ttttgccgtt gctcttgacg
     1861 taatccttcc atgattgcac ggacagcaaa ttttgttccg caataaacgg cggaatcagg
     1921 ataaacaaca tgtcccgcca cagaatcagt tgaaatgata tgcccagatt tttgtttttc
     1981 catgattgga agtacggcgg aaattccatt taacacaccc atgatgttga tatcaagcat
     2041 attttgccat tcatcacggt gaccttcaac taatggagcg gttggcatga ttcctgcatt
     2101 attgtacaat acatcgattc gaccgaactt ttccatcgct aaatcgataa ctgcttggac
     2161 ttgtgcaaaa tctcttacat ctgctgtttg aatatatagc tcagcttctg gtaaagattc
     2221 ttttattgct tttaggcgat cttctcggcg tgcagctata actagtttag cccctttttc
     2281 tgctaatagc ttcgttgttg cttcaccgat tccgcttgaa gcgcccatga tgacaatcac
     2341 tttttctgat aaatcattca ttctattctc ctcctgtttt gaaaaatctc ttgcttatac
     2401 tcactatctc ttatttcatc ttactgttca aataatccgt tcgacaggtt tgacgatttt
     2461 ggcagggatt cctgcaacga tggtattgtc tgggacatct ttcgtaacag ttgcattcgc
     2521 tgcaacaatg gcattctcgc caatcgttac gcctggcaga atagttgctc ctgctccgat
     2581 ccaagcattt ttcttgatgt ggattggctt tactgacaag cctcttcgtt cttttggatc
     2641 ggttagatgg ttcaccgtga tgaggttgac acgaggacca atcaatactg aatcttctaa
     2701 acagatgcca cctaagtcaa cgaacatcac ttgacgattg ataaagacat ttttccctat
     2761 actaatatgt ttgccgaaat ctgtataaaa aggcagcaga acagtcaccg tatcatctac
     2821 tttgtcacta atgatatttt ctagccgttt gcggatatct gctggttcat ggtacccagt
     2881 attcagcgct gctaaaggct cttcgttttc cgcttggatc tgatggatca cttcaaagag
     2941 cggatccgtt actcgaatct ctttattgac gatttgtttt tcgatgaggc ttgtcatttt
     3001 gctccctcct tttatcctta ctttataacg gacggcttat gatgtctaat acttataaaa
     3061 catacttaac tattcctttt aactatactt agtggtatgc ttttttaaaa acaggaggga
     3121 aaaaatggat attcgtatac tgacgtattt tttagcagta gctagagaaa aaaatatcag
     3181 ccgagcagca aaatctctgc atatcacgca acctactctc tcaaaacaac tgaaagattt
     3241 agaagaagaa cttggtgtga cacttttcac tcgaggctcc cgagagatac agttgacgga
     3301 ttctggtcga tatctttatt caaaaggaaa agaaatcttg tcgttggtag aaaaaacggc
     3361 gaacaattta acaaatgacg caatcatctc tggagaaatc tacattggcg gaggcgaaac
     3421 taaagcaatc tctttcattt ctgatagcct tcattcgttg atcaaggcgc atcctgatat
     3481 ccagtttcat ttatatagcg ggaatgcaga tgatatcata gacaaactgg atagcggatt
     3541 acttgatttt ggactcgtaa tcgaaccgac aaacaaacaa aaataccagt catttcgact
     3601 gccagcccaa gatcgttggg gacttttgat acatcggaat catcctttag caaagcaaac
     3661 agtcatccat ccgcaggaac tgaaaactgt tcctttgatc atttcccggc aaacgtcagt
     3721 ggacagtcaa ctagctgaat ggctaggttt cagcatagat cagttatcga tcgtcggaac
     3781 atataactta ttgtacaatg cttctttgat ggtcgataac gggacttttg gtgcattagc
     3841 tctagatggg atcatcaata cttatgggac ggaaaaagta ttcatcccac tcgatccgcc
     3901 attacaagct tcactgaatc tcatttggaa aaaagaccag cctctttctc ctgctgctcg
     3961 taaattttta gatgtcctaa cggagaaata cagttaagta gagttttttt ccttcgcttt
     4021 cagcttttta acaacgaata agttaagcaa aagatccaat gattaaataa cataagtttc
     4081 ttaatttgtt ccttcctttt atttctgata attccagtaa attactataa ttataaaaaa
     4141 agaattgaca aaacataact gtctatataa aattgattac gttaacaata attcgtttca
     4201 tgtgactaga tcatactagg catgaaaaat ctctgaggaa catggtttcc ctaggaggtt
     4261 tttcgtgtct atttttgttt ggccgcaatc aactggtcta gcaaacgtga aaaccaaatc
     4321 ttcaaaaagg gtgcgcgcat gaaaatcaaa aagatcttta atcaaaatgc agtattggta
     4381 gatgacggtg gacaggaaaa agtagctatt ggaaaaggga ttggatttga cagaaaaaga
     4441 aatgatttga tttttgatcg tgatgtcgaa cggatatttg tcatggaacc agaaggtcaa
     4501 atcaagttgc aaaatttact taatcagatt gatgaacgct ttttatttgc agcagaaacg
     4561 atcattgatc atgcggaaac tgttttgatg gaaaagttga atgaacatgt attgatcgct
     4621 ttgaccgacc acattgcttt ttctgcggag aacattaata acggtatcgt catccgcaac
     4681 aaacttttaa gagaaatcga agtgctttac agtgatgaat tcagtattgc tcaatgggca
     4741 gtagagtatc tgacaaaaga gcttgggatt ccatatactt atgacgaagc aggatatatc
     4801 gcaatccata ttcacagcgg acgaagcggt cggaacaata accatcgcag tatccgtgaa
     4861 gtgacgattg tatctgacat catccattta gtggaatcag aattggaaac agatatccat
     4921 tcagaaccat tttcactgaa ttattcaaga ctggtgaatc atttacgact gcttctacaa
     4981 cgaacccatg cgcaacaata tgctgtttta gacacggaaa tcgtagaaat ggtcaaacga
     5041 aaatacccag aaagctataa gatttctaaa aaaatccgtg tgttgctgac caaagaatac
     5101 cagctcgcga tcacgaaaga agaactgggt tacttggcca tccatatcga gcgtttaaga
     5161 agcgcaatcg tacaaacaaa tgttaacaat catgaggagg aaaaaaattg aaagcatatc
     5221 tacaacgaat gggacgttcc cttcaacttc cagttgcggt cttacctgct gctgctttac
     5281 tagtagggat cggaaactgg tgggcatctt atagtaacga cattgtcgct cattttttac
     5341 aagctggcgg taacgcagtt ttaggtcaat tacctttgct gtttgccgtt gggttagcac
     5401 tagggatgtc agtggacaaa tcaggtgctg ctgccttggc aggtttggtc gcatttgaag
     5461 ttccagtaaa tgttttaaaa actgattcag tagctacttt attgaatatc aaaccagaat
     5521 cagttgatcc atcttttgct cagatcagta acgtatttat tggtattatt gccggattga
     5581 ttgcggcagc tctttataat cgtttccacg aagcaaaatt accgatggct ttatctttct
     5641 ttagcggcaa gcgtctagtg ccaatcttgg ctgcctttgt catgttgctg atcagtgcag
     5701 tgctactgct cgtatggcct ccggtttact ctgctttagt ttcttttgct aaattcattt
     5761 tgagtttagg ctggggtggt gcaggtcttt atggcttctt caaccgttta ctgatcccta
     5821 caggattgca tcaagcaatg aactccgtgt tctggtttga cgtagcagga atcaatgata
     5881 ttggtaaatt tttggcttct gaaggaacaa aaggcgtaac aggaatgtat caagctggat
     5941 ttttcccaat catgatgttc gggcttccag caggagcata tgctatttac agaaatgcac
     6001 gtccagaaaa caaagcaaaa acagcttcct tgatgttagc agcagctttt gcttcattct
     6061 ttactggtgt aacagaacca cttgaattct catttatgtt cgttgcttgg ccattatatg
     6121 tcttgcatgc aattttcact gggatttctt tagcagtgag tgcattcttc cattggacag
     6181 caggatttgc ctttagtgcc ggatttgtcg attacttcct atctttgaaa aatccgattg
     6241 cgaatcagcc attgatgttg atcgtccaag gattagtaac agcagtgatt tactactttg
     6301 gctttaactt tgctatcaaa aaattccatc tgatgactcc tggccgcgag gaagcagatc
     6361 tttcagatga cgatactgca acgactaatt cagacaataa atatgcggca caagcaagca
     6421 aaatctacgc tgcattaggc ggagccgaca atgtaacttc gatcgataac tgtactacac
     6481 gccttcgttt gcaagtcaaa gatacaggcc tgatcgatca aaataaaatc aaagccactg
     6541 gcgtacctgg tatgaagatc atcgacggaa aaaatgcgca agtcatcgta ggtacagaag
     6601 tacaattcgt tgcagatgag atggcaaaac tacatggtgg tgctgcaaca cgacctgcac
     6661 aaacaaacac cgtggttaaa acagaaacat ttgccggtgg aacgatcgaa caagatattt
     6721 acgctatcgc aaatgggaaa ctgattccaa tcactgaagt ttcagatgat gtattcgcgg
     6781 aaaaaatgat gggtgacggc tatgctgtat taccagaaaa tggagaaatc ttctctccaa
     6841 tcgctggtac gatcacaaac attttcccaa cgaaacatgc tgtcggaatc caaacagatg
     6901 ctggtatcga agtcttgctt catatgggca tcaacactgt tgacttaaaa ggcgaaccat
     6961 tcactttata tgtaaaagaa ggtcaaaaag tcgcaagagg tcaattgatt gctttagttg
     7021 atcttgccgc tattcaatct gcaggcaaaa acacggatat ggtcgttgta ttcacaaatg
     7081 gcgacaaagt ccaaagctta gaaatcgaac ccgcacgtga tgtaaaagca aatgataaaa
     7141 tcggctctgt ttccaacaaa gcctgagcca tcgtcttccc cagacttttt tatcgataaa
     7201 caaacaggag ctgagacaaa aatcttgtct cagctccttt ttccgaataa aaacggcgtt
     7261 ctcctgccaa ctccgcggta ataagtccag ccaaacgtaa catatgaaga gctatttttt
     7321 atttgactct tcttattact tgatgcagaa cagctttttt acaacctctg ataaacggtg
     7381 ttcaaaaacc agtttatcgg tatgaagtca aatttatgca aaaatgtgga aagccatttt
     7441 cacaaaattt ttcttcatac tcaaaactaa tcggcttttt cacaacctct ttatacatta
     7501 ttcctgatcc aatatttctt ttaatgctgc ttcaagagaa gttaatggtt ttcccaatac
     7561 tttttctaaa tcatcagagg aaatagctaa ctgatccttt ttaatatcat gttggatcgc
     7621 taaaaacatg tcagctgctt cttgagggaa tccggcttct tttagatttt cgacaaacgc
     7681 tgcatcactt gcttcaataa tttctaactt tttacctgtc gcacgctcta atgccttggc
     7741 taattcttca tagtcagtta gattgccgga taattctaag atttctggag agtcactttc
     7801 tactagtact tttgctgcag cttctgcata ttcacgtttc aatacccaac ctgtttttcc
     7861 atttcctgca gcataaacga attgaccagt ttttaaagcc tgcccaatga taggtaattc
     7921 attctctaca taccaattat tccgtaaaaa tgtatgggaa atacctgaat ccttaatcat
     7981 tttttcagtg aattggtgat ctggcgctaa tacactgatc gagtgatcgg catctgcaaa
     8041 acttgtataa gcgatataag ataccccagc tgcttgtgct gccttcacta cattcttatg
     8101 ttcggcttga cgatttcctg gaacgccaga gacaaaaagc aatcgatcta ttcctttcaa
     8161 cgcttctttc attgagtcta aatcatcata atcaccaata cgtatattaa atccagcttc
     8221 tttcagtgct tctccttttt cttcactacg tactagtaca tagatttcat tttgtggaac
     8281 catctttttt aagaattcca gcgcataacc accaaaacct ccagttgctc ctgttactaa
     8341 ataatgcata tttgccactc cctattcttt ttatttgtta agatttacag ctgtacattt
     8401 ataaattaca cctttatcat aaagagtgtg gtaagataag tcaagcaaaa aaaatgaaag
     8461 gatgacaact gatgaagtat tctctgcaat ttagtgatgc aatccatgta cttgcctata
     8521 ttgaaatgtt tcaagatacc cgatcacttt ctagtgagat gattgccagt agtatcgaaa
     8581 caaatgcagc aaatgtacgt aaaatcatga gtcagctcaa aaaatccggt ttgatcttta
     8641 caaaaaccgg aaaaccgcag ccttcccttg ccaaaagtcc agatcagatt tctttactag
     8701 atgtctatcg cagcatcgaa ggtaatacac agcttatcca agtcgatcca aaaacaaatc
     8761 caaattgcgt cgttggtgcg aatatccaag aagctttaga agaaagctac gctcggctgc
     8821 agaaaaaggc agaagaagaa atggctaaga tcacacttga tagtctgata cataaaatag
     8881 ccgttttgga aaaagaaaaa agaccagaaa acacaggcct cgtagagaga tttttataat
     8941 tctctctact gtgcctgcat ttttctgatc ggaatcgtct acataaattc ttttattctc
     9001 cgggggacaa aagccttgtc atgccccttt ttcctctcta atgctcaggt caaagcgctg
     9061 aagtcatgac gtcttgataa acggtgttca agtagctgac cctcggcatt agttctgaaa
     9121 aatgcaaaat atggaaagct atttcccatt tttcctctct aatgctcagg tcaaagcgct
     9181 actttcacga cctctatatt atcatggttt attgactacc ggtatccaga tttctcccat
     9241 atatttcccg ttggtaactc ccatttcgac tacagcattt ggacccccaa cataggcgta
     9301 atcttctact tctcttaaaa catgcccaaa ggccgtattc gtcaaatcat ttcttaattc
     9361 ttcaggcgat tctgctaaag cgattacaac aagatacgtt ccttttggga atgggatgat
     9421 tcgggtagtg tttggaacat cctgactcgt ttgaacaccg acatagtaca tcattttccc
     9481 gttatagggt tcgttgactg taaagaaata atgattttca gcagcttgtt tcaatgcatc
     9541 tattgttcca ttaccgataa ctttttgctc aaatgcttgt ttctctgcag ccaaaccgac
     9601 aaaatccgta ttgctactct tcaattccac accaataccc gtcaccgtaa aatctggttt
     9661 ttcttcgatc ctatactttg ccatttctcc caccctttct attatattaa gttcatctta
     9721 cataaaatta aagggaaatg acacgattac gctctttttt ctagggatat ttttattttt
     9781 taactatttt tttgctttca ctgcaatcca aatctcactg taaacattac tgaaatcctc
     9841 tccccatttt gtaaaagaga tctcgggagc ttcgactaac tcataatctg aagaaggaag
     9901 ccattctgca tagattttcc cccaagtatc ttgtaaagtt tggggaaatg gccctttatt
     9961 aggaaaaatg gcccaagtgt gttcttctat agcaagcatt tctaaatcag cgtacggatt
    10021 ttcttttgtc gtagcaaagc caatcatgtg catcaaactg cccttttctt ccaagcgggc
    10081 tccatcaaat ccataagaaa cgttgattac ttgatttgga tataaatctc ctaacttatg
    10141 catctcttcc ctttgagtct ccgtgatcgt ctgcgctaac ttttgtattt cttgattgac
    10201 tccttcaaat ttgacaggaa cttttttcgc cacacctgcc acataaaact gttctttctt
    10261 ttcgatacga aattccatcg attttcctcc tctgacatca ataaaaaatg tcaagcgtgg
    10321 aaaagatttt tgtatgccgt atctgctgat ttctgaaggc agatatcctg accaatcacg
    10381 aaaagctctt gaaaaacctt cgactgtttc atatccatat ctaaacgccg tttctgtcac
    10441 tcttgatcca tctaggagat cgcgattagc caaagccaat ctacggtttt tgatatagtc
    10501 cattagcgac atccctgcaa taaacgtaaa gatgcgtttt aattgatatt cagagattcc
    10561 tgtattttcg gctattattt tataggaaag tccttctgtc aaatgttctt cgatgtaatc
    10621 gatcaattgg ttcagatctt taagcatatt tatcttcctc cttgcttgcc tttttatcat
    10681 attatcaaag aaagtcaatt acttgatgaa tcaggaacca atttagtcag atgtagaaaa
    10741 tgttgtatgg atctaaagaa aattctcaag cagcgtttcg taacggataa ttggtattat
    10801 ctgcctatct atgttttacc tttagaaaaa taaattttgg ggaagacaca ttctaagtaa
    10861 acaaagcggt aaaaatatct tttattccct tagttaatcc gcttactacc tactgtacaa
    10921 agcaaattgt tgttcaaact gctgatcaga acgctgcttg gatttttctt tgcgatacat
    10981 catacctctt ttatcaattg gaacaattat ttatttctaa caaaatcact tcatttttgt
    11041 cagataaata aaaaacaaaa aaagttccat ctttaaaaca cgctattctt ttactttttc
    11101 tcacattctc taagaaaaga aaaggagaat gaatttttga caattcgagc aaaagtgatt
    11161 atcaaatatg taaaatcagc tacttttttc ttaaacgttt ctttatacta aatataaata
    11221 agtgtttcat gaaaggagaa ctagatgaat aattggcttg agtattttcc tgaaaacgtt
    11281 ttggaacgcg gttacagcta tcatctgcac ggttttgtcc gtcatttgaa ctatacaagc
    11341 aagtacttgt ccgcaacagt ttctggcacg gaagattata aggtagtgat cacttgggat
    11401 gaaaagacca acatgacttg tgattgtcta tatgccatag aaggaaaaaa atgcaaacac
    11461 atggctgctg tcttattcgc ttacgaagaa cgtccaataa aaaaatccaa ctactcactt
    11521 tccgaactct cgtcccttgt ttcctccgct agttcctctc ttgtacggga gcttttaaca
    11581 gagattctta tagaacatcc gcaatttatc gaacgcttca aagttaagat gccatttcat
    11641 gcaataaatt attcagataa attaacaact atcatccata aatacgatca tattatcaag
    11701 aaaaacaaaa acaggaaaac agcgaagttc atcatggaga tgcgaaagtt catccaagaa
    11761 gcagttgaat cgctcattca gcaaaatgcc tatcttcccg cattcgaatt gatcaatgaa
    11821 gtgattgcta ctctcgaaac attctattgg gagcctgagg atgaacgaac ccttcttctg
    11881 atagaggact gttattattt atggaaagaa ttattagcag aagcacctca tgcagaaaaa
    11941 aggcaaatgt tctcttggtt cgtctgccaa gtcgatcata ctgacgcctc ttattcaaaa
    12001 cgctactcca taaaaatcct gaaagaagac tttcgggaaa aagaattttc taaccaaaag
    12061 aaaaaaatag ataaaaaaac taaagaaacg gtaaagaaag acgatttcaa tgaaaaatag
    12121 caagtaaacg ataacaacca tcctaaatcc acaagaaata cagcagacct tgctttaaaa
    12181 acacgaatca tgcaaaggat gaagtaaaga agacttaacg cgtacccaat attagtcatt
    12241 tgactattag gtacgcgttt tgttttataa gtcatgacaa atttccttcg ttgctgggta
    12301 gattttccgg cattgttgat agattttatg atacttttct acattttgta agagaggttc
    12361 cactgcatct gtatattgga taaaattctg acaacaactt tcaggtgtgt cgaaccactc
    12421 taatctttaa agataaatat taaaataacg attaataata ttaacagtct ttatttttca
    12481 ccaccaaaaa cctatctttg catatactaa atagacgtat atacacagat aggtttgtct
    12541 tgtgaaaaat aatgattatc cttaccaaaa aatgtttgtt tttctacatg ttcgtactta
    12601 tcaaaaaata tgatccttat tgaaattact ggctatttat attccaacga cttcttgtgt
    12661 taatttttgt tatctcatag taaattatgt ttcattttca cttaatttag ttgtcacgtg
    12721 ttcgtcaatt cgtaaaaacg gtaaatatat aaacacactt gcggctataa ttaatatttg
    12781 taaaacagct gctcgccagt ctccggctgt tgctaaaaat cccgatataa ctggtggcgt
    12841 agtccaagga atttgcacta ctacatgatt aattaatccc acagcagttg caaaatatgc
    12901 tactgtcaaa gaaaaaatcg gtgcaagcac aaagggaata atcagactga tattaaatac
    12961 aataggcaat ccaaaaataa taggttcgct tatattgaat atagcgggag ttaccgataa
    13021 tttagcaaag tccttataat cttttctacg actgaagata aatattgcaa tcaataaagc
    13081 aatcgtattt cccgatccac ccattaccgc aaaggtatct ttaaaggcca tattgataat
    13141 atgtggaatt tgctctttgt ttgcataagc tgccatattt tcttgcatat tctgaatcaa
    13201 aaacggatct aacaatgaac ctgatattac tgcttgatgg ataccaaatc caaaaaatag
    13261 attagcaata gatgtaatta acaaaaatcc tggtaaacca gttgtaattt tacttaatgg
    13321 ctctgtaact atcgtggtaa ttagcttatt tatatccata ttaaacaatt gaatgattat
    13381 aaatgacaat aaagcaaacg aggtaatcgt aataatgatt gggattaata cattaaaaga
    13441 ctttaaaaca gcgggaggaa tatttccgga tagctgaatc tgtagtttct tattattaga
    13501 aagtttaata aataattctg tagccgctaa tccaacaaat atacctacaa acattcctga
    13561 agctcctgta taggatatag gtatcacacc cgtcacttca acagctttat ccgcattttc
    13621 aggagtaaac atcatctgca taggtgttaa tacaacaaat gtcgaaatag caactaatac
    13681 cgctgcaata tgatttgtat aatttctgct tgcacacata tgataagata ctgctgctac
    13741 aactaataat gtagttatgc ttaatgtacc attggcaatt gaagtaccaa tttgttgtaa
    13801 cgtggtcaat gtactagtat cgacaatttc ccccatgaat cctgttggtt ctaagatcac
    13861 tgaattaata agtgtcacaa atcctgctaa catcataaat ggcataaatg tagcaaacgc
    13921 atctcgcaaa gttcttaaat ggatctgatt ccccaactta gtcgcaacta taccaaattt
    13981 ttcaaataca atgtccttcc tgctactttt gcccatattt tttctccttt atgcttattg
    14041 taatcgatta ctttttccac ccaaataata catcacttat caaa
//
LOCUS       DAZKNH010000063        14053 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000063 DAZKNH010000000
VERSION     DAZKNH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.063"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..259
                     /locus_tag="ACG8MG_001828"
     CDS             <1..259
                     /locus_tag="ACG8MG_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HFQ0375868.1"
                     /translation="IRPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFS
                     LAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            371..1774
                     /locus_tag="ACG8MG_001829"
     CDS             371..1774
                     /locus_tag="ACG8MG_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0375869.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            2123..2755
                     /locus_tag="ACG8MG_001830"
     CDS             2123..2755
                     /locus_tag="ACG8MG_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFQ0375870.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3343..4782
                     /locus_tag="ACG8MG_001831"
     CDS             3343..4782
                     /locus_tag="ACG8MG_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0375871.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            5077..5295
                     /locus_tag="ACG8MG_001832"
                     /pseudo
     CDS             5077..5295
                     /locus_tag="ACG8MG_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            5544..5759
                     /locus_tag="ACG8MG_001833"
     CDS             5544..5759
                     /locus_tag="ACG8MG_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375872.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5976..6232)
                     /locus_tag="ACG8MG_001834"
                     /pseudo
     CDS             complement(5976..6232)
                     /locus_tag="ACG8MG_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6419..7330)
                     /locus_tag="ACG8MG_001835"
     CDS             complement(6419..7330)
                     /locus_tag="ACG8MG_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0375873.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7349..8353)
                     /locus_tag="ACG8MG_001836"
     CDS             complement(7349..8353)
                     /locus_tag="ACG8MG_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0375874.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8350..10473)
                     /locus_tag="ACG8MG_001837"
     CDS             complement(8350..10473)
                     /locus_tag="ACG8MG_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0375875.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10478..12925)
                     /locus_tag="ACG8MG_001838"
     CDS             complement(10478..12925)
                     /locus_tag="ACG8MG_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0375876.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12912..13301)
                     /locus_tag="ACG8MG_001839"
     CDS             complement(12912..13301)
                     /locus_tag="ACG8MG_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0375877.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13361..13864)
                     /locus_tag="ACG8MG_001840"
     CDS             complement(13361..13864)
                     /locus_tag="ACG8MG_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0375878.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
BASE COUNT         4360 a         2741 c         2551 g         4401 t
ORIGIN      
        1 cattagacct gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta gctttgaaaa
       61 aaatacaaag agaatagtag gtgtacaact ttgtagtcaa gaagatttat ctggactatt
      121 atcaatgttt tctcttgcta tagaagaaca aattacatta gacaaattga aaatgttaga
      181 ttatttattt catcctagtt ttagccaacc ctataatttt attttaaagg cagtaacagg
      241 attaaacatt gaaacataat cgttactaaa tgaaatcaag aaatatactg tattactcaa
      301 ttcattgaaa atatttatca taatttttaa tagaaaacac tattaaagag aaaaaagaga
      361 ggtaaatcaa atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact
      421 ccttatagca acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa
      481 agaaagaacc gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact
      541 tttgacaact tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc
      601 atcaagtgaa agtagtacga cttcaaatgc cgagtcttca acaacttcgt caagtgaaag
      661 tagtacgatt tcaagtaccg aatcttcaac aacttcaagt agtacaagtg aaataacttc
      721 ttcgaaagtg acaaaaccaa accatataga atcttcaaaa aaggaagcgc caaaaacgga
      781 aggacaaaca aaaacttttt cgacaccaca gcaaatagaa tcaacagaat ctttccaatc
      841 acaaccaata ctatctggta atgaagaaat tcattttgac aaaaatgaat ctgtagaaaa
      901 ttttattatg aaaattggtg agtctgcgag aaaaattggt cagaaatatg atttatatgc
      961 ttctgttatg attgcacaag cgatcttaga atctgccagt ggtcaaagcc agttagctca
     1021 ggcaccgaat tacaatttat ttgggataaa aggaacttat aatggaaact ttgtcattat
     1081 ggtaacaaac gaagatttgg gtaatggaac gctttatacg acccaatcaa aatttagagt
     1141 gtatgaaaac tatgaggaaa gctttgaaga ttatgccaaa ttattaacta agggaattag
     1201 tggaaataaa gatttttatg caggagcatt aaaagctaat agtaaaacat atcgagaagc
     1261 aactaaattc cttacaggac ggtatgcaac agatacacaa tactatttga agctaaatga
     1321 attaattaaa acctacgatt taaccaacta tgataaagaa gttaaaggtc cacaaatgag
     1381 taagactgga taccaagtgc ctattcataa ttattcaata accagtttgt ttggaaatcg
     1441 tggaaatgaa tttcaccgag gacttgatat ggcagctaca cagggtgagc cgattcatgc
     1501 agttaaagct ggtaaggttg ttaaagcaga gtttcactac tcttggggaa attacgtggt
     1561 gatacaacac gaagatggat caaccgcgtt atatgctcac cagcaacaat acctagtaaa
     1621 aactggagat aatgttttgc aaggacaaat aattggctat gtaggaagta ctgggaatag
     1681 tactggccca caccttcatt tggaaatttg caaagatagt agtctttctc agagcatgtt
     1741 agttgatccg aaaaccttat tattcggaga ctaaaaaagg taatcaatct gacaaagtaa
     1801 caagaaaatg gttggattct tagacctttg atcaggatat tgagactgga attgatatct
     1861 cgattacgat gggtttgaca aataaaacaa gtccattatc gaatatcgag tgtattattg
     1921 ccgtctttat gctcatattt tgagcaggtt tcactagatg aaaacaattt atctatcttg
     1981 aggatttcgt gtacgtatcg ttcagtcttg tattgtaaat tagtcataaa atcagactaa
     2041 gaaaaattgg aaatattttt ttctgattta ttaaaagtta attgaaatta tatggtactt
     2101 ctttcttggg agggtaggtg cagtgaagga aagatatggt caccttactg ttgttgagga
     2161 gattactcat aatggatata aaaaatatcg ttgcctatgt gactgtggga atgaaacgat
     2221 tgtatatcga tcaaacttac tatcaggtag aaccaaaagt tgtggatgtg gaaattataa
     2281 aaataggcat aaatataaaa atttgacaaa taaaactttt ggtaagttag ttgcaatcaa
     2341 gccaactaaa ctgcgaagtg aaggaactgt tgtttgggaa tgtcaatgtc tgtgtgggag
     2401 aaaaaagtta gtatcggcaa agcgacttct aaaaggtgat gtaaaaagct gtggatgttt
     2461 gaaatcaaaa aagcatcaca ttgaaaataa aaaattcgga ttacttactg caaagtatcc
     2521 tttggacaat aactgttcat ggaaaacaaa atgggtttgt cattgtgagt gtggcagtat
     2581 gtgtgagata agttatagta acttattttt tggacatact caaagttgtg gatgtttaaa
     2641 aaggcaagaa acaaggacat gtgttgaagg gacagtaata gagtgtctaa cgtcaaaatt
     2701 accgaaaaat aatacaagtg gtgttaaggg agtttatcgt tctaaagaga aatagattgc
     2761 gtatataact ttaaaaaaaa agaggtacta tttaggatcg tatgattcta ttaagagtgc
     2821 agctcaagct agaaaagatg ctgaaaataa attgtttcaa cctttgattg acaaatatcg
     2881 aggtaatcat taattattgt ggataataat taacagtgat atataaacat ctataatata
     2941 ttattcatac tattgatatt tagtataatc ctatggaatt gaatatcata gcaatgtatt
     3001 tgaaaagcaa attggaaaac aaataggtgc aacaccataa atatgaaaaa attgggaaaa
     3061 agtagaatgg aaatttgctt acgtctcacg agatagctca cactattaat ctgaagaaca
     3121 atgaagtcga cttttagaaa ttgctagaag aaagtaaagc gaattatcac tagttactat
     3181 agagtaagtt caaataaagc atctccaact tacatggtta catgaggata tcaaatcgaa
     3241 attactcaag cttcctatgg ctagagtcgc gagaagttgt tgggcagtag acataggtta
     3301 tattgttctt agctacacga tcattagaaa ggaaatccta ttatgtatag ttatcaagaa
     3361 gatgcaaaag tccaaaaaaa tcgctggtgg attttgattg ctatatctat gtttaccttc
     3421 atgtccacat tggatggcag tattgtcaat attgcactac ctaccatctc agatgatatg
     3481 ggcataccaa tgaatcaagc ggaatgggta gtgtccgttt atctgatgat tgtctgttct
     3541 tgtttgttgc tgtttggtaa aattggcgat agccgtggga aaatcaaagt atttcggatt
     3601 ggtacgctga tctttgtctt gggatcgctg ttgtgtgggt tcaatcattc gctaggtatt
     3661 ttattgttcg cacgggtggt acaagccatt ggtgctagta tgactatggc tacaaatgcc
     3721 ggaatcatca cggaagtctt tcccatacat gaacgtggtc gggcattggg agcaataggt
     3781 gcgtttgttt cattaggctc gattgctgga ccaggtcttg gcggcttgat tttgtcccaa
     3841 ttttcttggg cgtacatttt ttggatcaat gtaccggtgg gaatcgtaac gatgttgatt
     3901 gctgaaaaat tcttaccaaa agatatcacc atgagcggcc atacaatcga tggagggggc
     3961 tttgctgcca ttgcggctac gattatcagc ttttttggag caatcttttt ggggcaagaa
     4021 agtggctttt tccatccagt cccccttgta ctgttcgtaa tttcagtgat tgcctttgtc
     4081 agctttttaa aagttgaaaa gaaagcagcc tctccattga tttctttcgg tatttttaag
     4141 aacaagattt ttaccatgag cttgattaca gccttgttga ttttttcttc caacttcttt
     4201 gtcaatgtag tgattccttt ttatctgcaa aatgctcgcg ggttgagtcc tagctatgct
     4261 gggctgttga tgatggtctt tcctttgatg atggtcgtcg gggcgccttt aagcggctac
     4321 ctgaccgacc gggttggccc ggagattttg gtattagtag gcttgggctt attgtcggta
     4381 acccaggtca tgtatatgtt tttgtcagtg gactctcctt tgtggctata cattgtcgct
     4441 actgccatca tgggtgccgg caatgccttg ttccagtccc ccaataatac gatggttatg
     4501 agctcggtca ctatcgaaaa cttgggactg gcaggctcga tgaattcctt tgcccgaaat
     4561 ttggggatgg tcagcgggat ttcgctttct acgacggtct tatatcaagc gatgtctgct
     4621 aagatgggcc agcaagtaac taactatatc gcaaatcggc cggatgtctt tttatatgga
     4681 atgaagatta cctttttagg cagctttgtt ttgtgttttg ttgccttttt cctgactatc
     4741 tggcgtatca aaaaacaagg aggtattcag aaaaatgtct gaaaaaacca ctgttgaaga
     4801 atttttggag gcctgcgtat gggtgagtgg gatttccatg gatgatggaa gatgaagtga
     4861 acttctaaaa gccgaaaaac tcaagtatta tcttcaaaat actagtgctc aatgagatgc
     4921 ttgatttgat cacaagaaga ctgcgaaagc cgctggcaac cgcaagctag gtatcccacc
     4981 gatggactac gttttagagg ctactggtta tgaatacgga gccaacgctc tcatctgcat
     5041 aagaatacat cacccagatt atgtaaagat tttcgatact tctgtcaaaa aatcattaag
     5101 atagataaat gatttccgtc tagtcaaatt ggttcagaat gtagacacac agatggtaag
     5161 aagtcactca caattcgaga atggacttgt cctatttgcc gtatacatca taatcgaaac
     5221 gtcaacgata gtatcaatat tttgaccaag ggctatgaat acaagcattg ccttagaaaa
     5281 catcaagaac cttaggaaca acggggatag cttggttaat aagagatacc gctgtttagg
     5341 cttgcaagta tactctttta tcaagaattc cgtggcttta gtcatgagag gcttaatact
     5401 atgaaaattt tctttagcat ttttgattta ggttcctttt ctaattataa taagatgacc
     5461 actacagtaa cctactgaaa tatataaaca gccactgtag taccattttc aaagtagtct
     5521 atgagaggag aatcttcatt cttatgaacg ctggaattgc ggatatgaat ttaataaaaa
     5581 aaacccttaa tgattttact tccaattcaa tatcaaaagg tactggtatc aatttaagca
     5641 caataaaaaa gctgaaaagt ggtgaaagat cagtggaaaa gctgaatctt ttagatgcta
     5701 taaaaataac ggaatttgct atgaaaaatg gaaaagctga gattgaaata tggagataat
     5761 tatcgtctag tgaattactt gccacttagc tgaatttaac gatccttggt gcttgaaggg
     5821 ggagattctt gggaatagag catacttgca agcctatgtc tttactaaca acgatatctc
     5881 ttattaacca agctatcccc gtagttctaa ggcttttact tttatctaag caataatcgt
     5941 attcaaagga aatttttgac ccttagaaag tctcatttta tcagacgtga gcttaggcat
     6001 tgctgaattt tgttaattcg gttttaggtt gtgctttgag cataatatga aagtattctt
     6061 tatcacgatt tcattcatct aaggtaatgt gatgtgattc tgaaattctt tcaaaggcag
     6121 tttttgcata agctgacatt acatcatcaa tgacatgttt acaatgtttc actactaaaa
     6181 taagggggta accaagaaga aaaactgaat gattatttgt atctaattcc atggtatatc
     6241 aaccatttcc tatatagact gattatgaca tggattaaaa taaaaaggca ctatgtctta
     6301 attgtcggtt ttcgagttac cgaatggcat tcatcaccca cctatagagg atgggcgact
     6361 tctgcctgaa ataagttaca ctacttataa cttctaataa aattagattg agtcattatt
     6421 atttaacatc aacgattttc cagtttccat ttttttgtaa tactaatttg tactgaaagt
     6481 agctagtggt ttttgataca ttatcaaggt attttatctt tgtactcact agaaaatttt
     6541 ttccgttttg tagataaact ggattctcta actccgaaaa ctttaagtca atattgattg
     6601 gtcgcatcgc accattttca acataatact ccaattcttt ttcagaagct gatggataaa
     6661 gtttaaaaaa tgtttctaaa aaatcggtga tttcttccgt tgttttggca tcaatttctg
     6721 aatcgttttc tacttgtttt gcttcatatt ttgccttgtc tggctttcca atcattgttg
     6781 gcaaactagt tactaaatga ttcctgtttt catcttcaaa gattgtcact cgatatgcag
     6841 aagaaattga ttgcgtcttt tttccctctg taatatgttg ctccactaaa aatgaaacga
     6901 caaattcttc cgagtgtttt tcaacatcta gaatttttac agattgaact tcggaactcg
     6961 tgggaatatc tgctcgaacc atatcttgga ttaaagcgag accgttttct gtaaggtact
     7021 gttcgagatt actcattctc ttttcaattg cttctttgtt atttttccat gaaaaatatt
     7081 ctttcgcaaa atcctctgta aaactttcaa cgccagaagt attcactacc ttctctttta
     7141 taaccacttt ttcatgagtt gtatgttgat caatggcggt gaaattttta taaacgccaa
     7201 aagcaagact acaagacaat aaaagccaaa gggcaagcac catttttcga tgcttaccca
     7261 cggatacaac tcgttctttt ttcttttttg ggctcttttc cttttgattc cgttcaattt
     7321 ttatcttcat tctttttcct cttttctctt atttgtatcg gccagctccg ataagatgct
     7381 gccgccaata gttttccgtt agattcgtcc accccactgg atctcctgca tgaaacatgc
     7441 gattctcccc tgcgtaaatg cccacatggg tcacataagt accggcatca taggttgaat
     7501 gaaagaaaac taaatcacct ggtttggcat cttttagatc aatatgagtc atggcatcat
     7561 attgatcctg agccacccga ggaagtttca ggtcaacttt ggcataacac cattgggtca
     7621 agccactaca atcaaacccc gtttctggac ttgagccacc aaacacatag gccgttcctt
     7681 catacttaaa tgcctcatca aaaatttctt ggactatttt gtcatcaaac tgagttgtag
     7741 ttaggtattg cttcactaat aaacagtaaa acatattgcc ataattatac cgccacccac
     7801 cattttcctt aatcgcgatt gggttggggt aatcgacttt ttgaccacct gatttttcct
     7861 ttgaaaaaga ttcagctaaa gcaaaactat gcttcttccc gtttttcccg acatgatcta
     7921 gatacccgcc accatagtta taggcctgaa taatactatc ttgatcacac cctagttctt
     7981 tggaacgctt aaccaaagac gcaaaataga gacagccttg tttgatggat gcctcggtac
     8041 ttaatgaatt aggtggtaat ccggctgact cagaactttg cataacgtct tccattgtgc
     8101 caccagattc cacttgaata atcgccaaaa gaattggaat atactctgtt atatccgatt
     8161 ctttggcata cttttccacc attgcacggt gtttcaatac ttcttctgag acactagttc
     8221 cgccaggaga agtgccagaa ctaccatttc cctcactgtc ttcaccaaac aacaaaccca
     8281 cacacatcaa aatacccata aaagagatgg tcagaaaagt taatgaaata acacccactt
     8341 tcttcaagtt cattttttct cacctgacct tgattttgga cgatggttca ttcttttttg
     8401 tactttttga ttggtctgct ttggcagagt ggacctctgt ttcacttgtt ggaaagaatt
     8461 tttcttactt gctgattggc ttttcccttt tgaaatgatt tcttgactag ttagttttcg
     8521 tttgatttcg ggattcagat ttgctctcac aatccgcttt ggtgcttctt ttttcagcat
     8581 catatctttc tgaggttgag ctgctttatc tattggaata ttcgcccttc tttttccctg
     8641 acgatttgtt gcttcactca gtatcttgcg tttttcgtcc attttcgcat ttcttcgttg
     8701 tcgttgtttt tctcgcaaat ccactcgatc cgctttttct tggaacagtc cgcgcttgaa
     8761 ttcttctggt gcttttttcg acttttcgat tcctttatgg agattgtatt tcaaattcgt
     8821 tggcgtattc cttacctgat cctttacttg tttagtctta tctttcacca aagcttgggt
     8881 atccaaaact ttgccgattt ttctcccagc caaattcatt cgggattgtt tcttattatt
     8941 tgaaactgga ttctccttag ttaagtcaga tagtatttcg tgatcgttcg gtaatcgtga
     9001 attctttcgt agagaagaac cggatggctt aaatttggat tttgattgtt cctttgccaa
     9061 cttatttccg actgctgcac caccaccaaa agccaaagct cgtcccacat ttcgattgag
     9121 ttgccgcaat tttcgattca ttaacatttg cggttttcgc atgacgcgtc ttcccattga
     9181 ctggctatca ttactctgga ggttgaacat actcataatg tcgcccaatt tgaaatagat
     9241 accggcaaag gtcacaattt gcagaaaagc aaccatgaaa aatggatagg ttgccgaaat
     9301 attgaaaaac atcgtggaga tgctaaatgc agtcgtaatc accaaagtaa cacccgctcg
     9361 catcataatc gtattgaata aacgaataat cgccttcttt cctaaacttt cataagtcgg
     9421 caacatggac aataagaaac taattggcag aaacatcgca aagataatga agagaatttg
     9481 cgagaacaac ataatccctg ttagaagaaa aacaaagaag gaaatgccaa tattgaacaa
     9541 taccaaaaag accacggtcc ctaaacggtt catggtcttg gtaatactga gattcgcatt
     9601 gtcattgtct tcaatctctg ccttgaccgc ttcttcccga tctttgcctt tgttttcatc
     9661 tggactcaca gatagaatct tgttgacccg gtcttcacca atttcttcta tgttggaatc
     9721 atcaaattgc agtagtaacc aaggttgttg gacttgaatc gaaaacaaac tatcccgaat
     9781 caaatccaca ctatcttttc cttggctttc tgaattgggt acaacgatct cggttccaag
     9841 ggtaagggcc gcttcactaa catctgagct aaagtcattg atttttgtga tataggtagg
     9901 cgcataagca atgaacgaac ccgatagtag aaaaatcatc acaaaattaa tcacggctcg
     9961 aactgctttc gtggtttctc gttttaataa tccggtatac gccacataca cccccagagc
    10021 taaaatcatc aataatagaa acccaaaata aaatccggag gtctggagtc cattttcagt
    10081 aataccagcc aatgtttgga tattctttcc aatactttca gccgtatccg aaatgaaatc
    10141 taatttgtaa gcctcttgaa ccacataacc cgtggcgttc gacaaataca aactcaccgt
    10201 ccaaatgaaa ttagtaatgg cataaagacc atacatcacg cttttcccta agccatcacc
    10261 ccaattccaa ggcaaccaat cccaagagct atccacaaag aaatctagtt gatagttgtt
    10321 cagtgaatat ttggaatata gatttcctgc ttcaactgta tcatcaacta gccctgtcgc
    10381 ctcggctacc gtaccaacta aactcaatag aactatcaga gtgagtatgc taataaaaac
    10441 gattcccaca atccgaaaaa gtttcttttt catcccctca ctccattctc tcttgaacag
    10501 gtggacgggt atcaaaggca tggaagagtt cttcaaagac tggatgtacc tgaaccacgc
    10561 ctacccgacc atacaaatcc tgcatcaagc attggccatt ttccaaatct cgtaggcgtt
    10621 tttgattgct ttcatcttct cgatccaagc caaagaattc caaggttttc ttgatttcat
    10681 gaatatcggt actgcgaaac gcaaacttca acccgatatt attcttcaag cgttcatcca
    10741 gtaaatcatc actattttgc gtgacaaagt aaaccccggc attcatagcc cgcccagcac
    10801 gaacgagctt attcgataat gttttccctt gcgctacttg aagaaaactc cacgcctcat
    10861 ctaagtctac aattttgaag atttctcgat tactatggat aaagtccaag gcaaaagtgg
    10921 aaatgacgat tagcatggaa acactaagca attccatggt ggtatattct tcaaaagtgg
    10981 tttctgcatc tggtaaaacc aaatccgcta cttgaatgat atttaactgc ctatctaaac
    11041 taatcgactg ggtaatcgaa ccatctgaga aaagcaaatg ggcaaaatca taatcggtaa
    11101 aggactcgat gtgatccgcg atattttccg caactggtga gccttctttt cgaagctcat
    11161 taattacttg taataatccc cgttgattgc tttgggtaac cgtccgaatg gccttacgca
    11221 acactggaaa cttctcccca tctcgactag aaattcctgt taaaaaggtc agaatatcaa
    11281 ttgctagact ttcagaatcc ttctttttct ccataatcac ataaggatca agtaatcctt
    11341 gattttttgg cttactagtt aagttgagaa tattgatttc atccgcaatt tctggcaagg
    11401 tttctttcca gccaccccgt tccccttttg gatcgacaat cacagcttgt ccaccaaaaa
    11461 ggacggcgta ataaactaag agattgttac taaaggactt tccgccaccg agtgaaccga
    11521 ggaatgccgc tgctagcgca ttggtaactg aatccttgac cccttgtgag gccaatgccg
    11581 gtttcaggta aacattgcgc cccgtatcta cgttataacc aaagtaaatg ccttccaact
    11641 cccctaacat ctgagtggcg ccaaaaccaa gtccggcgag aaaatcagac gtaacatact
    11701 ggatataatc attcatgtat cgcttactag aaggtaagaa ttcttcatgt agccccatca
    11761 tatcgccaaa aggccggact agttttacat tggtatcatc ataaaaatct agcacttcat
    11821 cacaccggcg ctttagttca tctactgatt cggcacttac tcgaaccaca tagctcagtt
    11881 tgtacatgga ttctttggac tgatccaagg tagtttccaa ttcatctacg gaatccaatg
    11941 catctaagac attggaattt gtttcattgt cagattgata ggcatggttg tccaagtctt
    12001 ttaattcctt tttcttattg cgaacggtag caagtgcctt tttgttcgtc acaatctcca
    12061 cattcatact agtgtcaatt gggaatgtaa attgttgttg ctggtaatag aaaagttctg
    12121 aggagggaaa ctccatttcc cccacaattg tattaattgt taagtacgct acatatgatt
    12181 catgattctc atgttccatg cataagtacc ggggcttttc ttcaatcaaa caacgactgg
    12241 gacgtaataa gtcgtaacgt ttcaccaaag tttccgactt cagctttttc tttggtagct
    12301 gaaattcata ttcttcaaat ggtgtaccct tttcgccata gatatgttca atcaaataca
    12361 tcagatcact cggtgtgact cgtctgactt taaatcttcg agccaattta ctttccatta
    12421 gtttttcaag cttggtatat cggcgaattt cttcattgga taaggaaaca aaatcgccca
    12481 tcaaatgatg attcactcca tagacaaatt cttgaaagcc ggagaagaaa gatttcttga
    12541 gattctttag attgacctct tcatctgtcg caatcagttt gaagccaata aaaaagcgat
    12601 aatcaatctg actatcgcca atcatggata ctaatgcctc tgtctgtaaa tcaattcgtt
    12661 gtttcgctac ttctgttaat cgcccggtaa tttccttttt tgatcgttct tgcgtggcac
    12721 gaatactact ttctgtggca atttgtaagg catgaatctt gccttcacga ttttgcgcaa
    12781 tcaattgacg aaagttatcg tggacttgat atttctgttc aggtgacaaa aaggagtaat
    12841 tgtaaggtat taactcatag taggcaaaac attctccctc ttgattaaag acaagattat
    12901 tttcaatata cttaatcggg tacatagttc acactcctta ccagagtaat gggttcgtcc
    12961 catcgtttct tctgttcttt gactttcttt cctgcgaaag tgaccttaaa tcgtaagtga
    13021 tagctaataa aagatcgtaa aaatcccaat ggtttcttac cgtcaaacgt tttttgcgac
    13081 atgaaccaag taactgccac cggtatacct aagtatttta aaaaggcccc ctctatcata
    13141 gatagtggag gcacatgggc aaatacgatg acaaacagca aggataaaac aaaccaagcc
    13201 atctggttaa aagtcacagg aaaaggcagt tgaaagtcat taattgcata aatgaccttt
    13261 tcaactgccc aaatactggt gtagcttttt attttcttca tcaaatcatt cctttaaaaa
    13321 gaaggatagt ttagaaatca actacccttc aataattttg ttaatatgga tactcaaaaa
    13381 cacctcgact cgttaccaaa taattgcctc caatctccaa gtctctacca cacgcatcaa
    13441 aatcaatata cgtcttcaag tgttctggta cttctccaaa atcaccagct tctacaatca
    13501 agaaatatgc tacatcactc atatcatcgc aatctggata atagataata tcatccacat
    13561 gctctaccat ctcctcaaaa gaattgaaga aagcatgttg aatctcagaa gcaacttcac
    13621 caactggcgt tccttcaagt tcctccgcta agccacacaa ccgattaatt tcctcaattg
    13681 gcgtatactc atcaatctca aagggaagtt cataatcatg aattgcatat tcctcgtatt
    13741 cgtcatttaa cccaattcgt tctttcactt catcaaaatc gacaggtggg gtaaaccacg
    13801 cccccaccaa ttcaccttcg ttatatttcc cgaggttcgc aatataaaca cgcatctgtt
    13861 ccatcgtgct tcccccttac gcaccgacaa ttcggttaaa caaactgagt aagacatcct
    13921 tcaccccaga agcattaaag accaatccga ccgcaatcaa agcaatcacc aaaaagccaa
    13981 tcagtttgga aaactcccgc ttaaatccta aaaacaaacc aatgaccaca attgccagta
    14041 agactaagga ttg
//
LOCUS       DAZKNH010000064        13977 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000064 DAZKNH010000000
VERSION     DAZKNH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.064"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..460
                     /locus_tag="ACG8MG_001841"
     CDS             140..460
                     /locus_tag="ACG8MG_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375879.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="ACG8MG_001842"
     CDS             912..1112
                     /locus_tag="ACG8MG_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0375880.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="ACG8MG_001843"
     CDS             1259..4048
                     /locus_tag="ACG8MG_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFQ0375881.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="ACG8MG_001844"
     CDS             4095..4622
                     /locus_tag="ACG8MG_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFQ0375882.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="ACG8MG_001845"
     CDS             4643..5833
                     /locus_tag="ACG8MG_001845"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0375883.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="ACG8MG_001846"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="ACG8MG_001846"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFQ0375884.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="ACG8MG_001847"
     CDS             7966..8640
                     /locus_tag="ACG8MG_001847"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0375885.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="ACG8MG_001848"
     CDS             8637..8978
                     /locus_tag="ACG8MG_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375886.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="ACG8MG_001849"
     CDS             9008..9367
                     /locus_tag="ACG8MG_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFQ0375887.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9627..9749
                     /locus_tag="ACG8MG_001850"
     CDS             9627..9749
                     /locus_tag="ACG8MG_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375888.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            9891..11975
                     /locus_tag="ACG8MG_001851"
     CDS             9891..11975
                     /locus_tag="ACG8MG_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0375889.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            12286..13752
                     /locus_tag="ACG8MG_001852"
     CDS             12286..13752
                     /locus_tag="ACG8MG_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0375890.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4635 a         2430 c         2974 g         3938 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatgaacgtc
    12001 ctcttttttt tatatccttt tcttaatgaa aaatgtaaag aaaatagata ggattataaa
    12061 ataactaatg tttttaattt tgcagaaaat tcaaaatagc ttgaaattca ataaggtcaa
    12121 tgaaataaaa gaaaataact tatttttttg atggaaaaaa tatgaaaaaa cgatgtgtat
    12181 taaataaaag aatacacaca taaaataaca ctgtcttgta agtttgttaa aaattgaata
    12241 tgattacatc tggataacaa atttttatca taaaggagag aacgaatgaa caacaaacaa
    12301 ttacgctggt ttactgttgc gttaattgca tttaacatgg tttggggcat ggggaatgta
    12361 gttaataact atgctcagca aggagtatca gttgtcacct cttggctatt gattttggcc
    12421 atttatttta ttccatacgc attgatcgtt ggacaattag gatcagcctt caaggatagt
    12481 aaaggaggcg tcagttcatg ggtcgaaaat acgacatcta ataaacgact ggcatactat
    12541 gccgcttgga cttactgggt cgtccatatt ccttatttgg cacaaaagcc tcaagcaatt
    12601 ctgattgctg gtggttgggc agtccaagga aatggaaata tcgtaaatac gatgtcagta
    12661 caaatggtag cagttatttc acttattata tttcttgcct ttttatattt atcaacaaaa
    12721 ggactttcta ctttgaaagt gattggcgga ctggccggaa cagcgatgtt cgtcatgtcg
    12781 atcttattta ttcttttagc tgtaacggca ccgttcatca atccaacgat ggaatttgcc
    12841 acaccacata tgaatcaagt gaaaacatat attccaacat ttgatttcag ttatttcaca
    12901 accgtttcga tgctagtatt tgcagttgga ggggcggaaa aaatatcccc ttatgtcaac
    12961 tctacaagaa atcctgcaaa ggaatttcct aaagggatga tcttcttagc agcaatggta
    13021 gggatttgtg ctgtattagg ctcagtcgca atgggtatgc tgtttgctag cgataatatc
    13081 cctaaagact tgatggcaaa tggggcatac agtgcttttc aaattttagg taattactat
    13141 ggtatcggta actcattgat gatcatttac gcattgacaa acatgattgg acaaatctcc
    13201 gcattagcct tttcaattga tgcaccacta aaaatcttgt tagcagatgc ggatccagaa
    13261 tttgtaccaa actggctacg taaacgtaat cgtaaaggta cattggtgaa tggttatctt
    13321 ctgacaggta tactagtagg aatcattatt ttgttgccga tctttggtat caaagatatg
    13381 aatgaattag taaaatggtt gacgaacttg aactcagttg tcatgccaat gcgttaccta
    13441 tgggtgttct tggcatatat gatgttgaat aaagcttata agaaattcaa ttctgaatat
    13501 aaatttgtta aaaatccaaa aatcggtttt atctttggtg cttggtgctt tgccttcact
    13561 gcgtttgcat gtatcctagg aatggtgcca aaagtagatt atactgctga tcctaaagca
    13621 tggtggttcc aattattcac gaatatcatc acaccaattg tcttgatctt attaggtatg
    13681 atcttgccag caattgctcg tcgagacaaa aacaaagaaa ttactgaacc agaaacagtg
    13741 gttcagcctt aaaatagaaa tacttgttac aagaggttgt gacagaagct acttctggaa
    13801 caccgtgaat cagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac
    13861 aaaaatagct cctcatattt tgccgttttt tgagctaatg ctgaaggtca gctgattgaa
    13921 caccatttat tcgaaacaag ggactttgac acgacttttg tcacagtccc ttgtttt
//
LOCUS       DAZKNH010000065        13972 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000065 DAZKNH010000000
VERSION     DAZKNH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.065"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..766
                     /locus_tag="ACG8MG_001853"
     CDS             65..766
                     /locus_tag="ACG8MG_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFQ0375891.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQNPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            763..1884
                     /locus_tag="ACG8MG_001854"
     CDS             763..1884
                     /locus_tag="ACG8MG_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFQ0375892.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1899..3131
                     /gene="pepT"
                     /locus_tag="ACG8MG_001855"
     CDS             1899..3131
                     /gene="pepT"
                     /locus_tag="ACG8MG_001855"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFQ0375893.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3263..4171
                     /locus_tag="ACG8MG_001856"
     CDS             3263..4171
                     /locus_tag="ACG8MG_001856"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0375894.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4206..4478
                     /locus_tag="ACG8MG_001857"
     CDS             4206..4478
                     /locus_tag="ACG8MG_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFQ0375895.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4489..5535
                     /locus_tag="ACG8MG_001858"
     CDS             4489..5535
                     /locus_tag="ACG8MG_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFQ0375896.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5786..8395
                     /gene="clpB"
                     /locus_tag="ACG8MG_001859"
     CDS             5786..8395
                     /gene="clpB"
                     /locus_tag="ACG8MG_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFQ0375897.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8546..9232)
                     /locus_tag="ACG8MG_001860"
     CDS             complement(8546..9232)
                     /locus_tag="ACG8MG_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375898.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFK
                     SYKLEKVKNT"
     gene            complement(9210..9704)
                     /locus_tag="ACG8MG_001861"
     CDS             complement(9210..9704)
                     /locus_tag="ACG8MG_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0375899.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9724..10944)
                     /locus_tag="ACG8MG_001862"
     CDS             complement(9724..10944)
                     /locus_tag="ACG8MG_001862"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0375900.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11091..12926
                     /gene="lepA"
                     /locus_tag="ACG8MG_001863"
     CDS             11091..12926
                     /gene="lepA"
                     /locus_tag="ACG8MG_001863"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFQ0375901.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            13122..>13972
                     /locus_tag="ACG8MG_001864"
     CDS             13122..>13972
                     /locus_tag="ACG8MG_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0375902.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNR"
BASE COUNT         4658 a         2476 c         2906 g         3932 t
ORIGIN      
        1 tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta gaaggagtga
       61 agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact ttgtccctga
      121 atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg cattgatatt
      181 aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt atgaatcagc
      241 acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc ttgcaaatgg
      301 tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg gtatgggagg
      361 ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa gcggacaaga
      421 acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc tgcaagaaaa
      481 tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat atgaaatcat
      541 tgtagccgaa aaagaacaaa acccagttac ttacaatgaa atggagctga tgttcgggcc
      601 atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag agcttcaaca
      661 aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac gtcaaaaaca
      721 aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat cagcggacgc
      781 actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga aggagaccca
      841 gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat gacattagac
      901 gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct gattgctaaa
      961 catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca agaaaaaatg
     1021 tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa catggatatt
     1081 atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt ggagagttat
     1141 ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc tgtggatcat
     1201 gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg tgattatacc
     1261 ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg tgcacaacca
     1321 gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga ggttattttg
     1381 ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc ttatgaggaa
     1441 cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt aggacgagta
     1501 ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa agaagcgttc
     1561 caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt gaagcgcatt
     1621 gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca aagagcagat
     1681 gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag cgctgggttg
     1741 attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt tatcgagaaa
     1801 tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc tgaaacaaat
     1861 acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat cttttgccac
     1921 gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa acgactccat
     1981 cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa gagttaggct
     2041 tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta ccaagcaatg
     2101 ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca gattttaatg
     2161 cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg atcgtactag
     2221 atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa aattatgaag
     2281 gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac aaatcaggga
     2341 ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc cctcacggta
     2401 ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat aaattcgatg
     2461 ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta ggtgagttgc
     2521 agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa aatgtacatc
     2581 cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat tttcataatc
     2641 agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc ttccatttga
     2701 tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc gatcatgatc
     2761 gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag ttgaatcagc
     2821 gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac atgaaagaaa
     2881 tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc gagcttggta
     2941 tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc tcttacatgg
     3001 gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga ttcgaatttg
     3061 tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca gaattgaaca
     3121 caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca atgatggaat
     3181 gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc ctcttttttg
     3241 aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca aaagttcagg
     3301 gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag aaaagcagcc
     3361 agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg aattagtcaa
     3421 gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg gcacgattca
     3481 tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat tacttccagg
     3541 tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag aagaagctgc
     3601 tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca atgatgaagt
     3661 cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga tcagtcaaga
     3721 agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt tattgctgaa
     3781 gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag gaaaatccca
     3841 actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg acgactcagc
     3901 gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata ttttccgatt
     3961 tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg gcattactta
     4021 tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca ctcgaacgga
     4081 tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag gattaacagt
     4141 ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa gttggaagaa
     4201 gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt gtgaattttt
     4261 acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc tggcgtgttc
     4321 ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga ttgatctctc
     4381 gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc gctgcaggtg
     4441 tacttgttga tttgattctc atttattttt tccattaagg agcaggagat gtcgtctaaa
     4501 ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt tgccattatt
     4561 tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc taccagtgtt
     4621 gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga agggcgctca
     4681 atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg gttcttgacc
     4741 tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct gatctcggag
     4801 atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt gacagcactc
     4861 catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt ggtcatccaa
     4921 ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc tggtatcctt
     4981 ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa tgttcaaata
     5041 ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa atgggtggat
     5101 acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat ccaagacagg
     5161 actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg gtatgtactt
     5221 ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac catttcgttt
     5281 atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat tggttccttt
     5341 gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc agtttcttcg
     5401 cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt tgtgtatgtg
     5461 attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa agagaataaa
     5521 gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa agaaaaacgt
     5581 tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac aatcaatttt
     5641 gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg tatattttga
     5701 ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata agtgatctta
     5761 cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga cgcttcaaga
     5821 agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata tcgacattgc
     5881 ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct atacggatgc
     5941 cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg agtatccagt
     6001 tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct ttcgattatt
     6061 gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa cggaagtcgt
     6121 tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga ataaaaacgg
     6181 attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag aaagagtgac
     6241 ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg atttagtaca
     6301 gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa tccgtgatgt
     6361 catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag aacccggtgt
     6421 agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag atgtgcctga
     6481 aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg ctggagcaaa
     6541 attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca aaaaaagtga
     6601 tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag gaaaaacaga
     6661 aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg aattacattt
     6721 gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca aagcgttgga
     6781 acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga tcagtattct
     6841 gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg acaacgcatt
     6901 agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac cggacaaagc
     6961 aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact ctatgccaac
     7021 ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag ctgcactgaa
     7081 aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag aattagctga
     7141 acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag aagaagtcaa
     7201 ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag aagatgcaga
     7261 aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac ctcaattaga
     7321 aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga tggttcaaga
     7381 gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta tccccgtaac
     7441 gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac ttcataaacg
     7501 agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc gttcacgggc
     7561 tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac caactggtgt
     7621 cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag aagatcatat
     7681 ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc tagtcggagc
     7741 tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg tcagacgaaa
     7801 tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg tcttcaatat
     7861 cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag tagtagattt
     7921 caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc tggaaggcgt
     7981 gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc tattacgtgg
     8041 aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta caccattgag
     8101 cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac aacgtctgga
     8161 acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg ctgaaaatgc
     8221 ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg aagtagaaac
     8281 accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag taacgattac
     8341 tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg tctaatcttc
     8401 cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc tccttttttc
     8461 aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt cttgaagctg
     8521 cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat ttataacttt
     8581 taaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt aaggcagcac
     8641 ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt ccgacaaaca
     8701 gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg tcgcataaga
     8761 attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg aataagagga
     8821 acaaccaagg actggttttt gatatctcag catctttcat caagtaacca atcaatccat
     8881 agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc caaaaagaga
     8941 tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag aaaaaccagt
     9001 aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg acttgtggca
     9061 tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct tgagaagtcg
     9121 aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca ccaaaagcaa
     9181 gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc atctaaaaat
     9241 ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt caatttgatc
     9301 aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc ttgtagtgca
     9361 gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa gccgatcact
     9421 gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc gatcgtcagc
     9481 ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc agcaagagca
     9541 atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc ttgaggaaga
     9601 tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg gttatataac
     9661 aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac atcatccttt
     9721 ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct ctagtgactt
     9781 cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag cacggcgata
     9841 attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc ctataggttc
     9901 atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac cattattcca
     9961 aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa caaacggaaa
    10021 ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt cgatctgctc
    10081 tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa tatctccatg
    10141 tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat ttcgagcttc
    10201 ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc agtagaacag
    10261 aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct catcttcatt
    10321 ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac catggttttg
    10381 ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta atcgctcaac
    10441 cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa aggaaagttt
    10501 atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag gatacaaata
    10561 agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta gatcttgcat
    10621 caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac ccattttata
    10681 acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga tcagtttatt
    10741 ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg aacggataaa
    10801 agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt ggaacacata
    10861 ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta attcaagatt
    10921 ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga taacagtata
    10981 gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt aaattactgc
    11041 ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct atggatataa
    11101 agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct catatcgacc
    11161 atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg acgtctcgcg
    11221 aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga atcacgatta
    11281 aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat atctttcact
    11341 tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc cttgctgcct
    11401 gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa acattggcca
    11461 atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat aaaatcgacc
    11521 tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc ggaatcgatg
    11581 ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat attctcgagc
    11641 agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta aaagctttga
    11701 tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg atcacggacg
    11761 gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca tttgatgtta
    11821 cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg gtcggtgatg
    11881 tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg gatacagtaa
    11941 cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg aatccgatgg
    12001 tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg gaagctttag
    12061 aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct caagcattag
    12121 gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc caagaacgct
    12181 tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt taccatgtta
    12241 ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct gaacctgtaa
    12301 cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca aatgattttg
    12361 tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg atggattatt
    12421 tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt gtctttgact
    12481 ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat gaaatgtctg
    12541 gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa aaagtcgatg
    12601 cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca atcgttgaaa
    12661 aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca gctattggac
    12721 aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg gctaaatgtt
    12781 atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa gggaagaagc
    12841 ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc gttctgaaaa
    12901 tggatgagca agatagtgtg aaataactta taatcaatcg aaaaacctat taaataaacc
    12961 tttttgagta gatgcaacac tgaggattat ctgtataaaa gattttaaac tgtcatttgg
    13021 ttatcacggc gatactaaaa ttaacaaaat tggaatattt ttcttgaaga tttttagctg
    13081 tcagtatatg atgaaagcat ctcataggag gtgctttttt catggttgat aattcaagtg
    13141 gaagggttct taaatctcta agaaaagaga aaaaactatc tcaaaaaaag ttagctgatt
    13201 tagcaggtat ttcacaaagt actttagtta aatatgaaaa agggtcaaga aaaatcccta
    13261 aagacgtaga taatactcta agcaagattc tgaatataga aactctaata aaagatgagg
    13321 aagataagat tgagattctc atcggtaaac taattgcata cagagatatg aataagttac
    13381 ttaacaaaga gttagctgat aatataggaa ttagtgaagt attactcagt tatgtgttaa
    13441 atagaaagag aaatccgtca aaagaaatgc aaaaaaaaat cgatattttt ctattaagta
    13501 atgaaaaaga aattttgaag gagattaatc gtgatagtga gatatttagt ttatcaaaag
    13561 acgataaaat tgttatggga aaaagaatac gagaagtccg aaagaaccgg gaagagacgc
    13621 ttgaaaaatt tggtaaaaac tttaccatct atacaggcaa aaatgttata agtagatggg
    13681 aaaaaggtat taatattcca gatattgaaa aactaatgaa tattgcctat cttggtaagg
    13741 tcactgttcc ttaccttatg tacggagaag attataagaa tatacttcca aaagatgaaa
    13801 gagttagcga ttttaaaaag ataaattctt ttagtatggg attaaggatg aggaaaatta
    13861 ggaaagatta ttatctagaa agggaggagt ttggaaagct tttttctcct tcaatttcaa
    13921 aatggtccat agataggtat gagaatggta gagatatacc caatactaat cg
//
LOCUS       DAZKNH010000066        13962 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000066 DAZKNH010000000
VERSION     DAZKNH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.066"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2110
                     /gene="acm"
                     /locus_tag="ACG8MG_001865"
     CDS             <1..2110
                     /gene="acm"
                     /locus_tag="ACG8MG_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFQ0375903.1"
                     /translation="SLIPVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEK
                     KQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKN
                     LDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTG
                     DMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYR
                     GEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFEN
                     HSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTF
                     ELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELK
                     FSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDA
                     ETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNE
                     NKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPK
                     YRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTER
                     EKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVI
                     IGLLIVTICAGIFLQKYKKV"
     gene            complement(2382..2867)
                     /locus_tag="ACG8MG_001866"
     CDS             complement(2382..2867)
                     /locus_tag="ACG8MG_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFQ0375904.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    2957..3302
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3347..3988
                     /locus_tag="ACG8MG_001867"
     CDS             3347..3988
                     /locus_tag="ACG8MG_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0375905.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4007..4642
                     /locus_tag="ACG8MG_001868"
     CDS             4007..4642
                     /locus_tag="ACG8MG_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375906.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4660..5475
                     /locus_tag="ACG8MG_001869"
     CDS             4660..5475
                     /locus_tag="ACG8MG_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0375907.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5794..7230
                     /locus_tag="ACG8MG_001870"
     CDS             5794..7230
                     /locus_tag="ACG8MG_001870"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0375908.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7419..9866
                     /locus_tag="ACG8MG_001871"
     CDS             7419..9866
                     /locus_tag="ACG8MG_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0375909.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10096..11163
                     /gene="rlmN"
                     /locus_tag="ACG8MG_001872"
     CDS             10096..11163
                     /gene="rlmN"
                     /locus_tag="ACG8MG_001872"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0375910.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11153..11359
                     /locus_tag="ACG8MG_001873"
     CDS             11153..11359
                     /locus_tag="ACG8MG_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375911.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11356..12459)
                     /locus_tag="ACG8MG_001874"
     CDS             complement(11356..12459)
                     /locus_tag="ACG8MG_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0375912.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13012..13560
                     /gene="aac(6')"
                     /locus_tag="ACG8MG_001875"
     CDS             13012..13560
                     /gene="aac(6')"
                     /locus_tag="ACG8MG_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0375913.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            complement(13808..13927)
                     /locus_tag="ACG8MG_001876"
     CDS             complement(13808..13927)
                     /locus_tag="ACG8MG_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0375914.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
BASE COUNT         4766 a         2331 c         2951 g         3914 t
ORIGIN      
        1 atcactgatt cctgttcatg tctatgcgga tgcaggcaga gatatcagca gtaatgtcac
       61 ttcgttgacc gtggacccaa cgaatattac agatggagga aacataaaag taaaattctc
      121 gtttgacgaa aaaaaacaaa atatccaacc aggtgattat ctttggatca attggccaag
      181 tgaaggaaat attcgcggtg aaggattcca aaaagaaatc ccattaatga ttgaaaataa
      241 aaatgttgga actttaacgg tcagaaaaga ttctgcgcag gttgtgttta atgaaaatat
      301 taaaaaccta gactcagtag aaggttgggg gcaatttgag atacaggcca gaaacgtaac
      361 cgataccggt gaagaaaata cgggaccatt caccgtgacg agcggtgata aaacagctac
      421 tgtaaatgtg acaaagccag cttctggttc ttcttctagc gttttttatt ataaaacagg
      481 agacatgcta cctgaagata ctaagcacat tcgatggttt ttgaatatta acaataacgg
      541 cacttatgtt gaacaaccag ttaaaatcag tgatgaaatc caaagtggac aaagattaga
      601 tccaagtacg tttgaaatta atcaaataca cttaggtgaa cagaaggttt atagaggaga
      661 agagggaatc caacaatttt tacaagattt ccccagcgct acatttaatt tttcagtaac
      721 tgataattac attgaaataa ctataccaaa aaactttgtc aatcttagga aaataatggt
      781 gagttataag acgatcatag aaaacccaga acaaataaat tttgagaatc attcggaagc
      841 ttggtttaaa gaatttaata aacctgctgt tgatggagaa agtttcaatc atacagttaa
      901 aaatatttca gcttctggtg gagtcaacgg tacagttaga ggcgaattga agattttcaa
      961 atatatcaat gacacagaga ttggtatccc aaatgtgact ttcgaattaa gacgagcaga
     1021 tgaacagcct attcaaggac agtctagtat cctgttgact tcgaatgaac agggagaagc
     1081 tagtatcaaa ggcctgcaag taggagacta tgttgtaaaa gaaaaagaag cacccaattg
     1141 gattgatttt gatccactaa gttcaaatga actcaaattc tccataaacg aaaatgatac
     1201 agagggagtc agtttaccta tttataacaa gaaaaaggtt acgaacatca cagcaactaa
     1261 aatttggaac ggtggaacca ctccaagacc gagtatttat tttaaattat tcagagcaag
     1321 tacaaacaat tgggaaccag ttcctgatgc agaaacaaaa cgattagaca atggaataac
     1381 ttcagtgact tgggaagata tccaacagta tgatgatagt ggaaacgaat atacgtttaa
     1441 agtgcaagaa gtagatcaaa acggaaatga ttatgtccct tcaggttatc aaaaaattga
     1501 aaacgggcta gtcgttacaa atgagaataa agaagtcatt tcagtctcag gacaaaaaac
     1561 atgggaagac aatgagaacc aagacggcaa acgaccgacg acgatcacag taaatctatt
     1621 agccgacggt caaccgatcc aacacaaaga agtaagtgaa aaagatgatt ggcgctatcg
     1681 tttcacaaat ctgccaaaat acagagacgg gcaagaaatc atctatactg taacggaaga
     1741 caacgtgccc gaatacagta cgacgattga cgggtataac atcaaaaaca gttatacacc
     1801 ggaaaaaaca aatatatcta taccaggtag ccatataaca ccgtggaaaa attttccaga
     1861 gaagacagag agagagaaaa ttagaaatct attctctcaa aaattgcaaa tatttgctca
     1921 tacaggagaa tccagcgaac aaagaagcaa ttatattagt aagagagctg tacctaaaca
     1981 gataacaaat aaatcaaaaa gcattcttcc taaaacaagt agtaaaaaat ctagttttgc
     2041 tgtaatcatt ggtttattga tagtaactat ctgtgctggg atattcttac aaaaatataa
     2101 aaaagtttag ttagaatata atattttttg ttaaatatga ctgaaatcaa aagaataaaa
     2161 ttccgattta taatcgaaaa aagaagcatt tttatatatg cttctttttt cgtagaatca
     2221 ataaatatgg atttggctaa ctatggaaga atactcgtat tagttttcag cgttcatact
     2281 agttgttaca ttgtttgatt ggccaaaaga atttctgata tattttgtgc taaataaaga
     2341 aaaagacgct tgaattggga aataagaaaa ctactctatc attacaaatt ttcataaact
     2401 ggcagatgtt ctaagtaaac aagtatcttt tcaaagtcgg aaaaatccat aatcaacgta
     2461 gtggtattga tatttggatg aaagcctacg gtatcttgac gatcaatagc agcatcgatc
     2521 acgacttgga tttttttctc tttgtcgtgc atcaatgcta atggagtcac tgtacctgct
     2581 ggaacgccta ggaggtcagg aagctgttct tcagaaacaa aagataagcg cttttctttt
     2641 aattgttcag ctagttgttt taaatcggcg tgcttttcat caggcaagat gacaagatag
     2701 atttgttttc cttttcttga tttcaataat agatttttta cttgcggacc aggtagggaa
     2761 aaagggacat ttttgactga cgtaatcgca ggatgatcga ctttttgaaa agagataccc
     2821 agttttttta gtagttcata agcttcttgt tcggttgcac gactcatcgc gcatcaccct
     2881 ccttttttta ttagtgtatc ataaaaaaga gttgacaaat gaaaaataat catgtaaatt
     2941 acacgtaaag ttaaaacaaa tgacatcgaa aagaagagta gccgttaggt gtttttagag
     3001 agtctctggt tggtggaaag aggcagcagt taaccgtgaa acacatcttg gagtcgattt
     3061 tctgaagcgt atagtaggaa aatccgggga gcccgttaca gctgcggtcg ttgctagatg
     3121 accagataag gctggctttt ccgtgaggag tcggtaaaaa aaggtggaac cacgtgatta
     3181 acgtcctttg atcttcggat caaaggactt tttgttttca tctgaacggc aacgactgtg
     3241 tgaggttttt tctgcaagga aaaaataaac agaggtggaa ccacgtataa tacgtcctct
     3301 tggcatttgc caagaggatt tttttatttt aaaggaggaa aagaagatgg agtatttgat
     3361 tgaagttatg ccgcaattat tagtaggagc actcacaaca ttgaaattat ttttatttac
     3421 cttgattggc tcattaccat tagggattgt tttagcatta ggattgtctt tgcattttaa
     3481 gccgctacgc tacttgcttc aaacatatgt atgggttatg cggggaacac cgctgttgtt
     3541 gcaactgatt tttatttttt atgggttgcc gacgattggg atcgtttttg atcgtttcga
     3601 agcagcactc attgccttta tcttaaacta tgcagcttat tttgcagaga tctttcgtgg
     3661 agggatacaa tcgattcctc aaggtcaata tgaagcagcg caagttttgc aattaagtcc
     3721 ctttcaaacg gtgacgaaaa tcgtcatccc acaagtgaca aagattgttt taccttctgt
     3781 agggaatgaa gttatcaact tggtcaaaga ttcttccttg atctatatat taggtttagg
     3841 tgacgtaatg cgtgctggga aaatcgcgat ggaacgcgac gcgactttac tgcctttgct
     3901 aggcgtggca atcatctatc ttacattgac tggtattcta acaatcataa tgaaacaagt
     3961 tgaaggaaaa ttaaattatt accgctaact gataggagga agaaatatga ttgaattgaa
     4021 gaaagtgaac aaagcatttg gtgaaaaaaa aataatcaaa gatttgaatc taatcattcc
     4081 agatgggaaa acattagcta tcgtaggtcc ttccggtggc ggtaaaacca cgcttttacg
     4141 tatcttggct ggcttagaaa gttctgacag cggagaattt tatttagacg gtgtaccgtt
     4201 caacccattc gagcttaaaa tgaaagaaca agttgtagga gtcgtattcc aagacttcca
     4261 gctatttcca catctgaata tttttgaaaa cattacctta gcaccaaaaa tggtattgaa
     4321 acagacaaaa gaagaataca ccaaaaaagc agaagaatta gcggcttcat tagggattag
     4381 tgatttattg aatatctatc cttatcagct ttctggcgga caaaaacagc gggtagctat
     4441 tgccagagca ttggctatga atccacgggt attggcttat gatgaaccaa caagtgcttt
     4501 agatcctgaa ttacgccagc aagtcgagcg tttgatccta tcattgaaag aagatggggt
     4561 aacccaaatt attgtcaccc atgatttagt atttgcagaa aatgtcggtg atcaattact
     4621 aaaagtttcc ccaattcatt aagaaaggag aagatagaga tgaaaaaaac cagttttcta
     4681 ttcgctttga tcgcaggtct tcttcttttt gcaggctgtt cgaacaaaaa aacttcagca
     4741 gataattggg cgactatcaa cgaagagaaa aaaatcgtta taggtttaga cgatacattt
     4801 gttcctatgg gatttagaga taaagacggg aagctcacag gatttgatat tgatttagcc
     4861 aaagcagtct ttgaacaata tgggattacg gtagactttc agcccattga ctggtcaatg
     4921 aaggaatttg aactgaacaa tggaacgatc gatttgatat ggaacggcta ttccaaaacg
     4981 ccggcaagag aaaagaaagt gcaatttaca aaaccttata tggaaaacga tcaagtgctg
     5041 atcacaccta aagaatcagg aatcacaaac tttggacaaa tgaataataa gagattaggt
     5101 gcgcaaaatg ggtcatctgg ttatgatgta tttacgaaac aaccagaagt attaaaagag
     5161 atagtcaaaa accaagatgc tgttttgtat gatagtttta atgaagcatt gatcgatctg
     5221 aaaagcagac gggtcgatgg tctgctgatg gataaagtat acgcagacta ttatttgaaa
     5281 caaagaaacg aactagaaga cttcaacata acaaaaggtt cttatgagag tgaggatttt
     5341 gcagttggtg cacgcaagtc agatagtaca ttagtgaaaa aaatcaatga agccctagat
     5401 caactagaga aaacaggtga attccaaaaa atatccgata aatggtttgg cgaagatgtt
     5461 acgccgagaa attaacaaat aggggactgt gacaaaagac gtgtcacagt ccctttttcc
     5521 gaataaacag tgttctcctg ccaactcctt gtgactcgga gctgaatggc aggctcacaa
     5581 cctcttggta aacggtgttc aaaagccagt ttttcggtat taagccaaat ttatgcaaaa
     5641 atgtagaaag ccattttcac aaaatttttc ttactactcg aaactaagcg actttttcac
     5701 aacctccttt acataatttt attttgttgt ggaaggggtt tcgtgttatt ctaaaagagg
     5761 aacgattgaa ggttatagga aaggaagaga tgaatggaac acaaagaact tacctcattt
     5821 ccaaaagatt ttctgtgggg ctctgcttct gcagcgtatc aagtagaagg agcttggcaa
     5881 gaagatggga aaggagaatc agtctgggat cgatttgtac ggatacctgg aaagacattt
     5941 aaaggaacga acggtgattt ggcagtagac cactatcatc gttataaaga agatattgct
     6001 ttaatgaaag aacaaggtct gaaagcttac cgcttctctg tagcttggac acggattttt
     6061 cctaatggac gaggagaagt caatcaagct gggctcgctt tttatgagag attgatcgac
     6121 gaattgattg aaaacgatat tgaacctatg ttgactttat atcattggga tcttccacaa
     6181 gcattgcaag atgaatataa tggttgggaa tcacgtcaga tcattgaaga tttcactcat
     6241 tatgcagaaa cattatttga ggcattccgc gggaaagtcc attactggat tagtttgaat
     6301 gaacaaaata tcttcacttc attaggttat ttgttagcag cacatccgcc aggtgtgaca
     6361 gacccgaaac gaatgtatga agttaaccat attgctaatt tagcaaatgc atctgtcatc
     6421 aataaatttc acgagttaga aatgcctgga aaaatcggac caagctttgc gtatacgcca
     6481 aattatccaa ttgacagtaa tcctgaaaat gttctggctg ctgaaaatgc agaagacctg
     6541 atggccaatt attggatgga tgtctatatg tggggaaaat atccgattgc tgccatgagg
     6601 ttcttagaag aaaaaggatg ggcaccaacg attgaagctg gcgatgcaga attgctagaa
     6661 tctgctaaac cagattttct tgggatcaat tactatcaaa cggcgacgaa tgcattcaat
     6721 ccattaaacg gagttggtgc tggaaaaatg aatacgacag gaaaaaaagg ttcatcagaa
     6781 gaaactggag ttcctggaat gtataaaaaa gttgagaatc catttgtcga acgaacaaat
     6841 tgggactggg agattgaccc agaaggatta aggatcggac tgcgtcgtat cacaagccgt
     6901 taccgcatgc cggtactgat tacggaaaat ggcttaggag agtatgacaa gttgacagaa
     6961 gataaacaaa tccatgacga ctatcgaatc aattacttac aaagccacgt caaagccatc
     7021 aaagaagcaa tttcggatgg agcagaagtc ctaggctatt gcacctggtc ttatacagat
     7081 ttgcttagct ggctgaacgg ttatcaaaag cgatatggat ttgtatatgt tgatcaagac
     7141 gaaacacaag aaggatcttt ggaacgatat aagaaagaca gttattactg gtaccaaaaa
     7201 ctgattgaag aaaatggaga gaacctttag ttcacatact taaaacaaga aggtacggct
     7261 ggatatggcc gtgccttttc ttgataaaat catttagaaa ttaagaaaaa ctgtaaaata
     7321 agctgaaaaa tgaaagaaag cgtcagttct ttcaaacaaa actttagttc ccttactttt
     7381 ttttgtataa tgaaacttga tgaaatgagg gataataaat ggcacaaaag aaacggccta
     7441 ctaaaaaaag gaaaaagacg aaaaaaacga aaaagcaaaa acagcagcag gaacaaatgc
     7501 tgacgattgg tgttgggttt gccttgattt tatttgctgt cttcggcttt ttaaaattag
     7561 gttttttagg aatattgatt gcaaacggct ttcggatcat tgcaggaaat acttaccaaa
     7621 tcctctgtct tttattagcc gtattaggat tttggattgt cattaagaat acagagtttt
     7681 caattggtaa aaaccgacgc tggtttggcg gcatactctt ttatttcggt atcttattat
     7741 tgttacatgc ccatttattt ggcaaactcc atactgggga accaaatatc atggggacga
     7801 catgggattt gttagccagt gatataaaac aaagtcaagt agacaataac gttggtggtg
     7861 ggatgattgg agcaatcctt tatcatttta cttacttctt gattgcacag cctggaagtt
     7921 atttagcagc agtgctatta ctggcaggag gagcattcct gttcagtaat ctggaaggct
     7981 atcagctgtt aaatggtatc caatcaattg gagaacgagt acaggaatta ttggaaggtg
     8041 atcctgccaa gcaagctcga aagcaagcgg caaaggaaga gagaatgaaa cagcgcgcag
     8101 aagcgaaaga agcaaggcgc ttagctgcaa aagaagctgc agaaaaagaa gctgcagaat
     8161 atgagaagaa aaaagcggtg agccagcaaa gggaaaatac tgctgatgaa tggcaggaac
     8221 cagaaaatac agagccggag cagctaagtt ttgttccaat cgacagtttt caagaaaata
     8281 tccatcctgc gaatctagag aaaccagtac cagatacacc aaaacagacc aatacagcag
     8341 aaggtttttc agatgagttg ccagaggatg acggtacctc attagaattt gagattgaag
     8401 cagaacagga aaatcaagac tatgaattac cgacagttga tttgcttgat tctataccaa
     8461 ctgtggatca aagtgatgag tataaaaaaa ttgaaaaaaa tatcggcgta ttagaacaaa
     8521 ctttccaaag tttcggtgtc gatgccaagg tcgtcaaagc cagcttagga ccatccgtca
     8581 caaaattcga agtccagccg gctgtagggg taaaagtaag taaaattgtg aacttaacag
     8641 atgatatcgc gcttgcttta gcggcgaaag atgtccggat ggaagcaccg attccaggta
     8701 aatcactgat tggtattgaa gtaccaaatg gaaagatcag tatggtttcc tttagagaaa
     8761 tcatcgaagc acaaccaaac cacccagata aattactaga agtaccttta ggacgtgatg
     8821 tttctggacg ggtacaaacg gctgatcttt cgaagatgcc tcacttactc gtggctggtt
     8881 cgactggtag tgggaagtca gtagcaatta atgggattat cacaagtata ttaatgcgtg
     8941 ctaagcccca tgaagtaaaa ctgatgatga tcgatccgaa aatggttgaa ttgaatatgt
     9001 acaacggcat acctcatctg ttgacgccgg tcgtgacgaa ccctagaaaa gcagcgcaag
     9061 ccttacaaaa agtagtacaa gaaatggaag aacgatatga aaaattcgcg gcaaccggag
     9121 taagaaatat ttctggttac aatgaatttg ttcaacagaa aaacttggaa aacggaacga
     9181 aacatccaac cttaccattc atcgtagtga tcgtagatga attagctgat ttgatgatgg
     9241 tagccagcaa tgaagttgaa gatgcgatta ttcgtttagc gcaaatggct cgggcagcgg
     9301 ggattcatat gatcctagct actcaacgtc caagtgtgga tgttatcaca ggaatcatca
     9361 aagcaaatgt accttccaga atggcatttg cggtatcaag cggtacagat tctcggacga
     9421 tcattgattc taatggggca gaaaagctct tagggcgagg agatatgcta ttcttgccaa
     9481 tgggtgagaa taaaccgatc cgtgtgcaag gagcattcat ttctgaccac gaagtagaac
     9541 gggtcgttca atttgttaca gatcagcaag aagctcatta tgaagagaaa atgatgccga
     9601 cagatgaagt tgaaacgaca ggtgctccag aacagccaca agatgagctg tttgaagaag
     9661 caaaagcact tgtcgttgaa atgcaaacgg caagtatttc ccttctccaa agaagatttc
     9721 ggatcggtta caaccgagca gcacgtttag tagatgaact ggaagctcat ggagttgttg
     9781 gcccgtcaga aggaagcaag cctcggaaag tattcattga gcaggaagaa acatcagaaa
     9841 cgttgccgga cgataatgac atgtaaaact tgtgtgatga agggttgaaa aacgaataac
     9901 caataaaaag acaaggcatg atgataccaa aagaaaaaat ggtttttttc catcttttct
     9961 aatacggtat gatgtcttgt ctttttagtt tttgtgagat taaagttcta ttcgatttat
    10021 ttgtgaagta ttgcattaag gaaatgaatc atgtatagta tgtagcgaaa ctttgagaaa
    10081 aagagggaaa atactttgga aaaacagtcc atttatggct tgacgaatga agaattaatc
    10141 aattggttta tagaaaacgg cgagaaaaaa ttccgagctg cccaagtttg ggaatggttg
    10201 tatcaaaaac gtgtaagtaa tttcacagaa atgacgaatc tatcgaaaca attgatcgaa
    10261 aaattatcgg agcattttat catcaatcct ctaaaacaga tggtcgtcca agaagcttcg
    10321 gatggtacag taaaatattt gtttgaactc cctgataaaa acatgattga gactgtactg
    10381 atgagacaag aatatggctt gtcagtttgt gtgacgacac aagtaggatg taatatcggc
    10441 tgtacgtttt gtgcaagcgg gctgctcaaa aagaaccgtg atctgactgc tggagaaatt
    10501 gttgcacaga tcatgatggt ccaacattat tttgatgagc gcaatcttgg cgaacgcgtt
    10561 tcacatgtcg ttgtaatggg gatcggtgag ccatttgata attacgacaa tgtaatggac
    10621 tttttgcata tcatcaatga tgccaaagga ttggcaattg gtgctcggca cattacagtt
    10681 tccactagtg gattagcaca caaaatcaag gaattcgcta ataacggctt gcaagtcaat
    10741 ctggctattt ccttgcacgc gccgaataat gaggtgcgaa catcgatcat gcggatcaac
    10801 cgaagtttcc caattgagaa attgatggaa gctgtggatg agtatttaga gaaaacaaac
    10861 cgtcgaatca cgtttgaata tatcatgttg aatcaagtaa atgatagacc agagcatgcg
    10921 caacaattag cagatctatt aaaagataag aaaaaattag catatgtcaa cttgattcca
    10981 tataatccag tcagtgaaca tgatcaatat tctcgcagtc caaaagctga tgtattgaga
    11041 ttttatgatg tattgaagaa aaacggcgta aactgtgtga tacgtaaaga acatggtaca
    11101 gatattgatg cagcttgcgg acaattgcga agcaagcaaa tgaaaaaaac agttgccaaa
    11161 taatcgtatc ctactcgttt tttcaattac cataaaagga gcagttttct taattaagga
    11221 actgttcaac ttaaataaga gaaaagattc tatagcgaaa gctataaagc tttttttgac
    11281 aagaaaaacc ctcatcaagc aaagaaatgt cctcaatttg atgacagcct gcttgatggg
    11341 ggttttagca attatttaac tgattttctt atatccttgc tggctttctt gtgtagcatg
    11401 ataagtttct acgtcaagaa tggcctgttt aaatttttct cgggtgattt cataaggcac
    11461 atgtttaata tcatttgttg ccatggcttt atctaagatt gcttctaaat cggtcgtgac
    11521 attgagattc atgtcggtta acgatactgg aagttggttc tttttcataa acacatagat
    11581 tttatcaaac aaatcgaact gctgatccat catcaataag gtcagtacac cataactgac
    11641 agatgctccg tgaaggtatc cctccatttt ttctagaaca gtcgtaccgt aatagaatga
    11701 gtgtgccata gcagaattat agttattgtt aactaaaaca gaaaccaatc cggtagtaac
    11761 aatgatatcc gaaacgactt gttcaattgc atatgaggga cgatcgtcgg cactatcttt
    11821 caatgcttgc gtaccataag tcaagagacg atcaatgcac atttttccga tttggattcc
    11881 taatctatcc gtgtagccta gatcatcgcc acgagcagaa aaagtagatt cgacttcttt
    11941 ggataaagca tcgccaatac ctgcccataa gtatttatca ggtgcttcgg caataattgc
    12001 tgtatcaatg aaggtgtgta caggtggttt tgaaggaaag aagagtccgt ccattacgcc
    12061 ttcttcgtta tagatgacac ataccgcagt gacgggcgaa cagttcgaac caatagtggg
    12121 aaaggtgaag actggtttag ctagtttttc accaatgact tttacagtat cgcaagcacg
    12181 gccaccaccg actgcaaaca ggacgtctgc tttttggaca ctttcatgct ggatcaactg
    12241 ttcaatattt ccataagttg aattaccccc gtaccagaga ctggcgacaa cctctatttc
    12301 ctgtgtcaaa gctactttta ttttgttttc tgcctttgaa agtgcagtct tccctccaat
    12361 cagcactact tttgtcccat attctttgac gattccattt gctttttcat acgcatctgt
    12421 tccaatggta taatttggta agaaaacact ttgactcata ttcatttcct tctttcttcg
    12481 ttgaaacggg ttaaaatttt aaagagtgat cgatctgact gcggctgtag atggagtaga
    12541 ggtgaaccta agacaggata agtgatgaat aaggtggcat gaacaccttg aaatcatcaa
    12601 taagtatcct aaaaacgaac aagtacaaaa acattgaaaa cgacaataat gaatttttgt
    12661 tcctcttagt atttctaccg attccctttg cttttttgta attatctaaa aattctaaaa
    12721 attaagatta ttatatgaaa acaggtttta tttgtcaatt cataattggt tttttttaaa
    12781 gcaaaagata atctatatca tgatgaacaa acagagtttt ttcctccaat tgatggcttt
    12841 cctgatcttt cctattttga ataaagtgtt tctgtataat ttttttattt tataagtaag
    12901 ataaataatg aaagaataga gatactgatt ggtagctaaa gatttttgag cgagaacatt
    12961 tcgttttttc aaaaggactg ttagaataag caagaacaag ggggaagacg tatgataatc
    13021 agtgaatttg accgtaataa tccagtattg aaagatcagc tttctgattt actgagactg
    13081 acttggccgg aagaatatgg agacagctcg gcagaagaag tagaagaaat gatgaatcca
    13141 gaacgaatcg cggtagcagc ggtagaccaa gatgagttag taggatttat tggtgcaatc
    13201 cctcaatacg gtatcacagg ttgggaattg catccattag ttgtagaaag ctcccgacga
    13261 aagaaccaaa taggtactcg attagtcaat tacttagaaa aagaagtagc ttccagagga
    13321 ggaatcacga tttatttagg tacggatgat ttagaccatg gaacaacgtt aagtcaaacg
    13381 gacctgtatg agcatacatt tgataaagtg gcttccatcc agaaccttcg tgaacatccg
    13441 tatgaattct atgaaaaatt aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc
    13501 tgggacaaac cggatatttg gatggcaaaa acgattattc ctcgaccaaa ttctcaataa
    13561 taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat
    13621 tacttcttga atttttgtga aattccaact tatttccgaa gaagctactt ttggaacacc
    13681 gtgtaccaga ggttgtgaaa aagtcgctta gtttcgagta gtaagaaaaa ttttgtgaaa
    13741 atggctttct acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
    13801 cgtttattca gataagggac tgtgacaaga cttttgtcac agtttctttt tgtttattct
    13861 tttttatctt tgtatggcaa taatagacaa tataacgaaa attatttaat ggcttttgga
    13921 agaccatgga aaaccttgta caaagctatt ttttctgaaa aa
//
LOCUS       DAZKNH010000067        13807 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000067 DAZKNH010000000
VERSION     DAZKNH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.067"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACG8MG_001877"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACG8MG_001877"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFQ0375915.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="ACG8MG_001878"
     CDS             complement(2260..2511)
                     /locus_tag="ACG8MG_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFQ0375916.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="ACG8MG_001879"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="ACG8MG_001879"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFQ0375917.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="ACG8MG_001880"
     CDS             3461..3679
                     /locus_tag="ACG8MG_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375918.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="ACG8MG_001881"
     CDS             complement(3968..4480)
                     /locus_tag="ACG8MG_001881"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFQ0375919.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="ACG8MG_001882"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="ACG8MG_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFQ0375920.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="ACG8MG_001883"
     CDS             complement(6933..7376)
                     /locus_tag="ACG8MG_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFQ0375921.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="ACG8MG_001884"
     CDS             complement(7377..8165)
                     /locus_tag="ACG8MG_001884"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0375922.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /gene="rnz"
                     /locus_tag="ACG8MG_001885"
     CDS             complement(8162..9103)
                     /gene="rnz"
                     /locus_tag="ACG8MG_001885"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFQ0375923.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="ACG8MG_001886"
     CDS             complement(9280..9867)
                     /locus_tag="ACG8MG_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFQ0375924.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="ACG8MG_001887"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="ACG8MG_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFQ0375925.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="ACG8MG_001888"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="ACG8MG_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0375926.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="ACG8MG_001889"
     CDS             complement(12784..13719)
                     /locus_tag="ACG8MG_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0375927.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3095 c         2435 g         4469 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataac
//
LOCUS       DAZKNH010000068        13540 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000068 DAZKNH010000000
VERSION     DAZKNH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.068"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..357
                     /locus_tag="ACG8MG_001890"
     CDS             190..357
                     /locus_tag="ACG8MG_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFQ0375928.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(419..2230)
                     /locus_tag="ACG8MG_001891"
     CDS             complement(419..2230)
                     /locus_tag="ACG8MG_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFQ0375929.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2227..2592)
                     /locus_tag="ACG8MG_001892"
     CDS             complement(2227..2592)
                     /locus_tag="ACG8MG_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFQ0375930.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2755..3150)
                     /locus_tag="ACG8MG_001893"
     CDS             complement(2755..3150)
                     /locus_tag="ACG8MG_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0375931.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(3168..4130)
                     /locus_tag="ACG8MG_001894"
     CDS             complement(3168..4130)
                     /locus_tag="ACG8MG_001894"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFQ0375932.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(4130..4342)
                     /locus_tag="ACG8MG_001895"
     CDS             complement(4130..4342)
                     /locus_tag="ACG8MG_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFQ0375933.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4363..5061)
                     /locus_tag="ACG8MG_001896"
     CDS             complement(4363..5061)
                     /locus_tag="ACG8MG_001896"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0375934.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(5081..5623)
                     /locus_tag="ACG8MG_001897"
     CDS             complement(5081..5623)
                     /locus_tag="ACG8MG_001897"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFQ0375935.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5755..6759)
                     /locus_tag="ACG8MG_001898"
     CDS             complement(5755..6759)
                     /locus_tag="ACG8MG_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0375936.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6756..7745)
                     /locus_tag="ACG8MG_001899"
     CDS             complement(6756..7745)
                     /locus_tag="ACG8MG_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0375937.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7742..8548)
                     /locus_tag="ACG8MG_001900"
     CDS             complement(7742..8548)
                     /locus_tag="ACG8MG_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375938.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8714..9670
                     /locus_tag="ACG8MG_001901"
     CDS             8714..9670
                     /locus_tag="ACG8MG_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0375939.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9747..10265)
                     /locus_tag="ACG8MG_001902"
     CDS             complement(9747..10265)
                     /locus_tag="ACG8MG_001902"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0375940.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10353..10502)
                     /gene="rpmG"
                     /locus_tag="ACG8MG_001903"
     CDS             complement(10353..10502)
                     /gene="rpmG"
                     /locus_tag="ACG8MG_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0375941.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10730..11176
                     /locus_tag="ACG8MG_001904"
     CDS             10730..11176
                     /locus_tag="ACG8MG_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0375942.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11371..13266)
                     /gene="asnB"
                     /locus_tag="ACG8MG_001905"
     CDS             complement(11371..13266)
                     /gene="asnB"
                     /locus_tag="ACG8MG_001905"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFQ0375943.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4233 a         2853 c         2302 g         4152 t
ORIGIN      
        1 acgaagttta cttttatggt gaaaacaaac aaagtaaaat ttcgtaaatt catatggtaa
       61 atttataata aaacatttag taaacatttt caagggtttt ttgcaaaggt ttacaaacaa
      121 acgtaaaact agcctttttt ctcttttgtt ccataataga tataacaaat aataaatgag
      181 gtggattcaa tgaaaaaatt tgtatctgga atattagtag gaagtctggc aacagccgct
      241 gccgttgctg gtttggttgc ttctgtgaaa aaaacagtga tcgatccaat tgacgaaaaa
      301 gaagcaatga tcgaagaaaa ccgtaaaaaa gcaatgcgta aacgcgtttc tcgttaataa
      361 ttatttaaaa atacgcatga agactttcta aaaaacagat aagtcatcat gcgtattttt
      421 atttgtcagt caatataagt tgtgctattt tatctgcctg tctcagattc catggatctg
      481 tataaggtct agctactgct gcagttagcc aatccacacc ttcaacaggt attccatagc
      541 aataaaagtc agactggatt tcccctgaag catcaatcac gcgattcgta cctcgctcaa
      601 catctatagc ttctgaatga taacttcctg tattatctga aaatgaaaaa gggcgaacaa
      661 gtttgttttc cttcaaattt tttatgacag gattacttga acgagaaaag cttgtttgtg
      721 gcaatcgagc ttcaatcaaa tagcgactgc tgatttcttt atcttgtaga gaaataacat
      781 agcggttgtg cttttgctga atatcaaatg gcggttcgat caaatgaaaa atacccgcat
      841 ctatcagtgc tgctaattct ttcgtccgaa tgattggcgg accgatcgtt aaaaagctat
      901 ttagatgagt aaaccatccc catagtattt ctttgtattc cttagcagaa aaaacgtgcc
      961 attcaatagc ctgccggatt ggatcacgcc agtcttttaa tgcatcaaat gttgaagcaa
     1021 atgctcctgt gcaattgcct ttttccgctt cttttatctg cccttcaata aatattcggc
     1081 tagcttctga aaagggctca ttctgatagg caagaggagt ttctaaatac gaccaatccc
     1141 atctataagg gattagtgct ggatgctttc cgagacactc ctctgaagag agggagagaa
     1201 aatcatgctc aaaatttttc tttggaatcg gcaagtgata ttcttcgatg attttcttgt
     1261 aataaaacaa ctcaacgtct tttttcagat agtcaaaaaa tgtttcgcca gtcaaaaatc
     1321 ccgactgatg ccattgctcc aggttttctt ttgttagtaa ccgtggccat gccattgctc
     1381 cgccttgctt ttgatttctt cctcgagggt aataagggag cccccttctt gatccactgt
     1441 atacaatcgg ttccttgcct gatggatgat agaccagttt tcccttttcg ttctcgaagc
     1501 gccctcctct tgatactgta aacaatccga cataatcaaa aaaacttaag cctagacctc
     1561 ttaaaaagac attttcttta ggcggtatct tcgaaagatc tacatctgca ggattagaag
     1621 gaagctgata cagtaaaaca tttttctttg cgtagtctac ccacactctt tcttcttctg
     1681 tctcatcatt ttcccagtga ccgcttgcta aaacaagctg atcgatagaa tagctttgct
     1741 gatctgtctg taaaagataa ccatcttttc ttttgcttac tttttttact aaagaataaa
     1801 caaactgaat agaagctgga aattcttctt gcaactttga gaaaaaccag cgttgataga
     1861 gtccgtataa acatcgagta gattgctgat ctttttccag acgattgatt tcttctataa
     1921 ttagttgctt atttttggtt accgtctgtt cgatataatc tgtagcaggt cctttactcc
     1981 attgaaacaa gttgggtccc tgtgttacca atcctccact ggacaatgtt tcatctgtaa
     2041 aaagcgtcac ttgttgagaa acagaattca ttaataactc cggtggctga ttcaaccgcc
     2101 aaactcgacc tccaggacca ttgggatcga aaagaaaaag ttcaagagaa tgtctgtcct
     2161 gattattatg gagaatcctt tctgctacac ttaatcctct aggtccggca ccaatgatcc
     2221 ctattttcat ttgcatgcct ccatttcgat aaattttttc aataaaacag catggttctg
     2281 atgttcattt tttgcaccat aaacaaaagt aatgactgga tgttctttca taagttgact
     2341 gagtttaaaa atagcttgct tcttttcttc ttcgaaaatc aattcttgct ggtatttctt
     2401 tgcaaatgaa gcaaatcgtt ctggtacatg gtcaaaagtt ttgcgtgctt cattactagg
     2461 tgcaatttct ttcaaccaca gatctaactg cgctttttct tttgaaattc ctcgtggcca
     2521 gaatctatcc acgagtattc tatagccatc ctctttttca gcatcaaggt aaacacgttt
     2581 taatttgatc atagttgttt ccttctttct accttttcgc ttagcttata tatgtagata
     2641 atagcacaaa ataaaaaaaa tgaacggcac aactatccat tgcgccgttc atgtgatctt
     2701 ggtcaaaatc taatgattga ttcggttttt aaagctgtca tacaatcatc tgttttatac
     2761 ttttttactt ttgatttttc cagaccatcc gtcatatcca cccttaagta aataaatctc
     2821 ttgataacct gctttacgta atttatttgc tgtacggatg ctcaaacttt tcttttgatc
     2881 gtaaatatag acaggctggt cttttcgaat agatcctaat gaatctttca aaacagtgta
     2941 agggatgttt cttgcaccta aaatatgccc agcatcaaac acatcttttt cgcgaacatc
     3001 gattacttgt gcttttctca ttccttctcg aaattcttct tctgtcaaag tcgttgctga
     3061 acgtttggcc attactcgta gatagacttc ccaaaggatg attgcaagca caatcaatag
     3121 caaaatgata ttaattaccc ataaaaccat gtaatgcctc cgttttttta agcaaatttc
     3181 aaggctaatg aggcagcacc aatgacacca gcttcattac caagctctgc taatttgatt
     3241 tttgttgatt ctcttacttg cgggaaagta aattctttaa aatatttctc tacacggcta
     3301 cgtaaaaatt ctcctgctgc cgatactcct ccacctaaca cgatacttga cggatttaat
     3361 gtattcccca agtttccgca agctaaacct aagtagaagc atacgcgatc aacaaccatc
     3421 aatgcaaacg gatcgtcttc ttgagccatt tcaaagatat ctttacttgt tacttcttct
     3481 ccgttgtcta gcattgcttt cagttttgaa tcccctgcgt attcttctgc caagaaacga
     3541 cctaggcgta ccacacctgt agcgctagat acagtttcta aacatccgcg tttaccgcaa
     3601 gtacattcga acccttcagg atccacagtg atatgtccga tttctcctcc agctccgctc
     3661 acgccgtgaa tcaaatttcc gccagcaacg atacctccgc caacacctgt tcctagagta
     3721 acgaatacaa cgtctggatc gttttctcca gcacctttcc atcgttcacc taaagcagca
     3781 acattcgcgt cattatcgat agcgaatttg attcctgttc ctgcctcgat cagttttttc
     3841 acaaattgaa cttctgtcca gtttaagtta tacgcaccaa taaccgttcc agcttcgctg
     3901 tccacgcttc ctggggtacc catgccgatt ccgataaagt cttcggcttt catattatat
     3961 aaattcaaac gatgattgat tgattcaatg atttcaggaa cgatatgctt accatcatcc
     4021 aaaatattgg tatcgatgct ccatttttgc tgaatctctc cttctggtgt taaaattgca
     4081 aattttgctg ttgttccacc taaatcaatc ccaataatct tcttgtccat tacaaatttc
     4141 ctttctttcg gctttcttca attcggtgct ctctttttaa tatgcgccac gctgtcatgt
     4201 atgtatcatg atcaatcaaa cggctatcat acagattacg caattcgata gtcattaatt
     4261 caatgtcata gatgcgtgca cccacataga taaagatgcc aaattttttg aacagctgct
     4321 gtacatcata taagctctcc atctttaagc acttccttca tattatacaa gtaaaccata
     4381 tttttttaaa cccagtaaaa tacatattac aaaaagaaat acaaaaacta gtgtagaaag
     4441 aatcctttgg tgtatcgaat agcttgtctg ttgtttcggt agacccaaaa cattccctaa
     4501 cactaatccg ccaattagcc caccgacatg tccccaaaca tctacagagg tatccagaag
     4561 accaaaaaca aatgtcaaca caataaataa aagaaaatgt ctgactagct gctggatcgc
     4621 tgggttatgt ttaaaatgaa accctaagat aaacaacgca ccgaacaaac cgaatatcga
     4681 agtacttgca ccaccagaca gaacattcgc ttcgttaaaa gcaaagcttg ctgtatttcc
     4741 cattatcccg ctgaacatat aaatcaagaa aaaacgccaa tggccataga ttgcctcgat
     4801 ttgctgcccc ataaaataca gtatcagtga gttcattacg aaatgcatca gtccaaaatg
     4861 gatgaagatt ggtgtcacta aacgccacca ttcattgaag tgaacaagat aaggaccata
     4921 cattccggca taataaggga tctcaagtcg cggaaataat tccatcatga ggaagaccgc
     4981 tgtctgtatc cctaaaaaag cataggtcca taacggtcgt cttaaccatg cacgcaattt
     5041 tatttgctgt tgataattca tgaaaacctc ccatttcatt ttagtcaata aacaattgtt
     5101 ggatcttctg atcaaagctc tccggttgcc attgttcatt caattgttca cgaaaaacaa
     5161 ggctacatgt ttttttatga aaattattta aataccggtc gtaataacca ccgccaaaac
     5221 cgattcgata accggctcta ttgtagataa tacctggtac aatgaggaga tcgatcatct
     5281 ccggcaaata gagcttctct gattgtggct caagaacacc aaaactcgaa cgtacaagaa
     5341 cggactgttc atccatttca taaaacgcca tctgacgttt cggcaaagtt ttaggtacaa
     5401 ctattttctt ttgttctttt tctgctcgtt ggataaccgg ctgtgtatcc agttcaaatt
     5461 cattcgataa agtcgtccct atcacttttg ctgacttcca gtactcgctg gcgaatagct
     5521 gtttatagat tcgctgttct ttttcctttt tcttgccact tttggccaga tttttcagct
     5581 tttcgattcc tttccggcgt aatcttactt tcaattggtc caaaaactca cccttccttc
     5641 tctcataaaa tggtatagga aaaaaagaaa aatagcaatc gaactttcaa ataaatgcat
     5701 aaatagaata aaaagaagac ctagaaatga ttggccttcc ttttttaatg aatttcaact
     5761 gttcagcgcg tgtttttgtc tgataaaaag tgcatacaga aaatatggcg caccaaaaag
     5821 ggcaatcaaa attcctgtag gtacttcccc acttggtaga atcactctag caaccatatc
     5881 tgcactaagc agaaaaatgg aaccgaacaa accgctcaga acaaaggtcc agttattttc
     5941 tttacgtacg actagcttcg cagcatgagg agctagtagt cctacaaaag ataaactacc
     6001 tgtcaccgct acactaccgc ttgccaaagc aacagctaac aacaaaagga ttttttgact
     6061 tttagccacg ttaaggccta atccttgcgc gcgttcttga ccaaatgata gcaatacaat
     6121 cctctgtgca taaagtcctc ctagcggaat taagataacc gcccatggaa gcaatgccca
     6181 aacataaggc cagctcgcac cccagattga tcccgctaac catgcattca caaatccata
     6241 attttcctta gataattgga tcgtgcatag caatgttaat gcagaaaggc cggcattaac
     6301 ggcaataccg gacaataaga ttcgattcat acccaaaaag ccatttcgat cataggccgc
     6361 aagatagaca cagcaagcag ctaaaacacc acctagaaag gctacaacta gcaatagaaa
     6421 cggcagcgaa tagtgcgaaa agaaacctaa ataaagcatc actaaaaatc ctgctccggc
     6481 attaatgccc aatatactag aatctgccaa ttcatttctt gttactcctt gcaagatata
     6541 accagaaacg ccaagacaag ctcctgctaa taaactgatc agtaatcgag gaactcgaaa
     6601 ttctctaaca atcagcgcca aagaagatga ttctttccct atgaacgtgg caaaaagctg
     6661 atccaggtaa ataaaaggcg taccaatcat taaactacct atagacagga tgacaagtac
     6721 aaaaagcaag aaatagaacg tccaatatat ttttttcacc gttgttccct ccttacttgt
     6781 atcagtaaaa acggaacgcc aatcaatgca atgactaatc caaaaggtgt ttcaaaaggg
     6841 ggattgatca tcctcgctgt aaaatccgcc aacataacca atccaccgcc aactaaaaaa
     6901 gagacaggta gaacataacg atagtccgtt ccaaccattc cgcgaacaac tggcggaacc
     6961 aacagcccaa taaatgaaac aggtccgact aacgaaacgg caatggctgc taacactgca
     7021 actagacaga aaacaatcaa ccgcgttcgt ttaggatgac ttccaagagc taatgcatgt
     7081 gtttcactca aatgaatcaa agatattgct tttcctaatg aaaaagaaac caaaacagca
     7141 agtatatata aaggaaacaa tactgctaat tgtttccatg tgatattggc agctccgcct
     7201 acaaaccaaa aagcaagatc ctgctgcaga ttaaagaatt gggtaagtgc ttggctgata
     7261 gcagtaaaaa aagaactaat agctacgcca gctaaaatca gctgtaaagg attcatgccc
     7321 attttcgaaa actgcaaaaa accaaatacg agcaaagtta cagcaaaaga acctgccaat
     7381 gacattagaa acacagaaaa atcagaagct ttaggccaaa aagcaaaact aagggccatt
     7441 gccaacgaag cacccgcgtt gattcctagt aagcctgaat ctgctaaagg atttcttgtc
     7501 actccttgca tcaatgcacc gctcaatgca aaacaactcc ctacaagaaa tgctcccgcc
     7561 acacgaggca aacgaatatt tcgtatgatt tggtgacttt gttttgatct atcaaaatca
     7621 aacaacgcct gccaaaaatc tgctttttcc acagtcgctg cgccatttga aagagcaaca
     7681 aaaaaaataa tcaaaagaac aaaacagacc aaaagcagga gtctattttt cttcatagtc
     7741 ctcataattg atcctcctgc tcttttggca acaaatcata ggttagaaaa actggtttat
     7801 ttgttcttgg aagcgtgaca atcgttgcat caatctcaaa tatttttttc atacactctt
     7861 ttgtgatcac ttcttcaggt gtaccttctg tgatcaactt accgtttttc attgctaata
     7921 aataatctga aaaacgtgaa gcatgattaa tgtcatgaat cgacatcaag atcgttgttt
     7981 gtttcatttg attgatttca gtcaataact gtaggatctc taattggtgt gccggatcta
     8041 aaaatgttgt cggttcatcc aagatcaaga tatctgtttc ctgagcaagc gccatagcga
     8101 tccagacacg ctgggtttgt ccaccagaca gcgttgcgat atcccgcata cgaaatccag
     8161 ctagacctgt agcatccaac gcccactcaa ttgcttcatg atcttgcgtg tccatgccac
     8221 tgaacagttt ttgataaggg tatcgtccgt aagatactac ttcttctact gtcatacctg
     8281 tagggtgttc actagtttgt gctaataatg caattttttt tgcaagttct tttgaactgt
     8341 atgtagagat atcagagtta ttgacaatga tctttcctga tttcacaggt agtatacggg
     8401 taaaactttt caataaagta gattttccac ttccatttgg cccaatcaaa ctagtgattt
     8461 tcccttcagg aatcgtaaca gacatgtttt caatgatttt tttgttttcg tagccggctg
     8521 caatgtcagc tgctcttaat ctcgtcattt cacccatcct ttcgctgaaa atcattctca
     8581 attatgacgc tgtttcaaca tttcgtcaag cgctactttc atatctatag ccaaatgaag
     8641 cggttgacag cgttcgaaat atcaggaata ataaccaatg agaaagattc tcaattatgt
     8701 taggaggaaa gaaatgaaat taaaaacaaa agtattcaca ttatctagca tattgctgct
     8761 ggctggagta ctgagcgctt gctccccctc ttcagataaa gaaacagcta caactgccaa
     8821 ttcgacgact tcatccaata gtactcatac agtcacagat acacttggac ataaagtaga
     8881 gattccaaac gcgcccaaac ggatcatcgg cagttactta gaagactatc tgattgcact
     8941 aggggaaaaa cctatcgccc agtggactgt tggcagtggt tccattcaag attatctgca
     9001 agatgattta aaggatgtcc caacgatttc ttatgacctt ccttatgaga aagtgttaag
     9061 ctttgagcct gatttgctgt tgatttcttc ttccgctact gtcgaaggcg ggaaatacga
     9121 acaatatagc aaaatcgcgc caacttatgt tgtaaaaaac ggaacagatg tcacatggga
     9181 agaacaacta aaagatgtcg gcaaagcttt ggcaaaagaa aaagaggcag aaaaaatcat
     9241 ttccgattat caaaaatcca tcaagaaaac tcgagaagaa ctagcagata aaatcgagaa
     9301 caagacagct gcagtgttgt gggtgaccaa caactcagcc tttatggtca gcaaaaatcg
     9361 gtcaagtgga agaatcgtat acgacgatct ggaatttggt gttcctgatc tagtggaaga
     9421 ggtctccaaa aaagccactt ctgactggtc tgctgtttcc tctgaaaagc tagctgaatt
     9481 agatgctgac tacatcatcc ttgtgaacag tgacaaagat gctgccatgt tcaatgaacc
     9541 aagctggaag aatctaaaag cagtgacaga caatcatgta ctgcaattcg gacctgaatc
     9601 tagttggcta tataacggac ctattgccag tcaggaaatg gtcaatgata tcaaagaaaa
     9661 actaaattga ttttaactaa aaaaagcatc ttcacgatta gtcgtgagat gctttttttc
     9721 ttattaacaa actagacgca tagattttat tcttcttttg aagcaaccct attttttact
     9781 ctttcccatt tttgaaaacg atgggcatat agattttctt gatccgttat cccctcatac
     9841 tcctctataa gggcgaagtc tgaaaaatca atattcccaa tatacgtatc gccttcaaac
     9901 gtgctatcga tcaatgttcg ccagatccga ccagtgtaag gaagaagttc ttgaaaaatc
     9961 cgactaccac ccgaaacata gatatcttct gagatcgttt gactatacga caaaagttca
    10021 tcgatcgaat gcattacttc cgcattttct ttcgtgcgaa aatttggttg cgttgtcaac
    10081 acgatgatat ggcgataagg aagtgaaaga tcccccattc cttcataggt ctttcttccc
    10141 atcactaaaa ttcgatccat tgtatgttcg cgaaagaaac gcatatcatt tggcaatcgc
    10201 catggtaaca aaccattttt gccgatcaga ccatttttat cttgtgccca catggaaata
    10261 aacatattat ccgctcctta tgattttaga tgttttgaga taaaaaaagt ctcaattctt
    10321 tgaccagcaa ggaattgaga actttaaaaa cattattttg tttcacggtg caaagtaact
    10381 ttgcgttcgc gtgggcaata tttttgtttt tccaaacgat caggattgtt acgtttgttt
    10441 ttgcttgtta gatagttacg ttctttacaa gaagtacact ctaaagtgat gtttacgcgc
    10501 atgtttttcc ctcccatttt tacaagaatc ctctaagatg actctcagac ttgattatct
    10561 taccatgatt ttttctagat tgctagtgtt tttcacgatt ttgttaagta aaactacttg
    10621 acgataaata caacggtttt acttctcaaa caaaatcttt ttctttttgt ataaataccc
    10681 ttacacttgt ttatatagat tatgtttttt aataaagggg gatactaata tgccatggaa
    10741 catgaaggac tttcctgcat caatgaaaaa cttagataaa ttgacaagaa aaaaagcaat
    10801 cgacatcgca aatgcattat tagatgaagg gtaccctgat aaccgtgcga tcccaattgc
    10861 cattgatcaa gcaaaaaaat ggtatgaaga tgctagtgaa tcagaaaaac gaactttcga
    10921 aaaagaaaaa aatccaagca agacggatga acacgatacg aatcctcgtg ctggaaaact
    10981 gcttgattca gatgtgatcg tagaatatga ggaagaacaa tggatcgtga aatctaaagg
    11041 ggcaaaaaag gcaagcaacc attttgatac aaaaaaagaa gcaatcgaaa aaggaaagca
    11101 agtagcacaa aacaaagaat caactttgtt catttataaa aaagatggga caaaagaaaa
    11161 agaaatcgat tattgaacat atgtaaaggg gttgtgacga aaatcgtgtc acaaccccct
    11221 tatgttcgaa acaatggtgt tcctctgcca actccgaacc ataagtcata atatctataa
    11281 aacgggaaga gctgtttttt gaaattgttt cttatcactc agtgttaaac ggtaggagaa
    11341 aacctctttt ttattgatat ttgattttca tcaaacagtc acatagtcga ttgcttctgc
    11401 ttgcggaatc gtttcatcta caaagtagac tttataccat gtcaagaaac tgaagattgt
    11461 ccagattttt ctttgctctt ctttacgtcc ttcgtgatgg tcatctagca acttcatgat
    11521 tttttcttga tcaaaaaatt cttttgcaaa atctgcttcg aataatgctt tgacttgttc
    11581 gtagccgtgt tcttctttca accaagcttt gattggcaca gggaatccta atttcacacg
    11641 attcgaccat tcttccggaa gatgtttgtt cgcagcttgt ctgaaaatat cttttgtgtt
    11701 gttttgattc aataaaaact tacttggtat ttgttgagcc aatttcatga cttcgatatc
    11761 caataaaggt actcggactt ccaaagaact tgccatagaa agtttatctg cctttaacaa
    11821 gatatctttt ggcatccatt gatgaagatc gacatattgc atcttattga cttcatcttt
    11881 gattccttct gttttctcgt aatgaacagt catgatttca tccacagatg gtgctgtttg
    11941 atattttggt gttaacactt ctgatgcttc tgattcttcg aacactcgag catgaccgat
    12001 aaaatattct tttgcaggag caagtgattc atataaatgg atccgtccat ggaagttttt
    12061 catttttccg attttacgtc ctaaattata gcgtgttcct tttggtaatt ttttcaatcc
    12121 ttgagcaatc acacggatga attttgaatt cgtatggaat ccatacgttt ggtatcccgc
    12181 aaacagttca tccgcccctt ctccagattg gactacccgc acactttgtg aagcaagatt
    12241 tgctaaaaag tacaaaggta cgcaagaagg atttgaatca ggttcatcta agtgatactg
    12301 gatcaaaggg aatgctttga acgacatgtc tccgtcgatc actgctgctg tattatctaa
    12361 gtccaatagt tcggttagtt ttctggcttc aatcgcttca ttataggttt tatcatcaaa
    12421 cccgattgag aaagaatgat ctggtttcaa tacagaggtt acataactgg aatcaacccc
    12481 gctggataag aaagaaccaa cttctacatc actgaccgta tgtgcttcaa tagaagcttg
    12541 aacggtttcg tcgatttttt cgatccattc ttgtcgggaa tgcgtttctt ttgtttcaaa
    12601 atctgcatcc caatattgct gaatgtctat ttttccatct ttatacgtat aataatggcc
    12661 ttcaggcaaa cgatagacat ctttgaagaa tgtttcgccg tttaatggag aatattggaa
    12721 agtcatataa ggtttcaacg cttctttgtt caattctttg ttaaaatcag gatgaggcag
    12781 aaaacttttg atttctgaac caaacataaa tgttccgttc atttctgcat aataatatgg
    12841 tttgatccca aagtggtctc ttgcaccgaa taactcattc ttttcattat cccaaatagc
    12901 aaaagcaaac attccgcgga tcttttgcaa taattctgtt ccccattctt cgtaaccatg
    12961 caaaaggact tctgtatctg catgtgtttg aaaaacgtgg ccagctgcaa tcaattcttc
    13021 tctcaaaggc tggtaattat agatttcgcc attaaaaata atgatctttg tccgatcttc
    13081 attgtaaata ggctgagtac cgccctctaa gtcaataata cttaatcttc tgaatcctaa
    13141 agcaacatgt ttatcgatat attctcctga actattaggt ccacggtgaa cgattcgatc
    13201 catcattgta ttaataattg tttttttgtt gtccttgtcg ttaacaaaac ctacaatacc
    13261 gcacatttaa ctttcacatc ctaatataaa attactgttt atttcattca tatgcatctt
    13321 atcatagaat aaatagcgga cgaatatttt ctctcttaca atttcgtcac gtgaaaacaa
    13381 aagatgaaag tttctgtttt tacagaaaat aaagtcccaa tatctaattt accatgtttt
    13441 cttttttgag gctaccttat tctcttcgtg ttaaaaaaat attagtggat gctatttcca
    13501 aaaaaaccag caaaaaaaga cacgacctaa agatcgtgtc
//
LOCUS       DAZKNH010000069        13346 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000069 DAZKNH010000000
VERSION     DAZKNH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.069"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1364)
                     /locus_tag="ACG8MG_001906"
     CDS             complement(45..1364)
                     /locus_tag="ACG8MG_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0375944.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="ACG8MG_001907"
     CDS             complement(1361..2014)
                     /locus_tag="ACG8MG_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0375945.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="ACG8MG_001908"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="ACG8MG_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(2820..3539)
                     /locus_tag="ACG8MG_001909"
                     /pseudo
     CDS             complement(2820..3539)
                     /locus_tag="ACG8MG_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(3490..3699)
                     /locus_tag="ACG8MG_001910"
     CDS             complement(3490..3699)
                     /locus_tag="ACG8MG_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375946.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            complement(3681..3749)
                     /locus_tag="ACG8MG_001911"
                     /pseudo
     CDS             complement(3681..3749)
                     /locus_tag="ACG8MG_001911"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            3976..5249
                     /locus_tag="ACG8MG_001912"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="ACG8MG_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="ACG8MG_001913"
     CDS             complement(5356..6387)
                     /locus_tag="ACG8MG_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375947.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="ACG8MG_001914"
     CDS             complement(6394..7224)
                     /locus_tag="ACG8MG_001914"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0375948.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7221..8036)
                     /locus_tag="ACG8MG_001915"
     CDS             complement(7221..8036)
                     /locus_tag="ACG8MG_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0375949.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="ACG8MG_001916"
     CDS             complement(8033..8857)
                     /locus_tag="ACG8MG_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFQ0375950.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="ACG8MG_001917"
     CDS             complement(8847..9947)
                     /locus_tag="ACG8MG_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0375951.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="ACG8MG_001918"
     CDS             10125..10910
                     /locus_tag="ACG8MG_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0375952.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="ACG8MG_001919"
     CDS             10943..11326
                     /locus_tag="ACG8MG_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0375953.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="ACG8MG_001920"
     CDS             11402..11533
                     /locus_tag="ACG8MG_001920"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0375954.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="ACG8MG_001921"
     CDS             11502..11738
                     /locus_tag="ACG8MG_001921"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0375955.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            11750..11836
                     /locus_tag="ACG8MG_001922"
                     /pseudo
     CDS             11750..11836
                     /locus_tag="ACG8MG_001922"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(11816..12787)
                     /locus_tag="ACG8MG_001923"
     CDS             complement(11816..12787)
                     /locus_tag="ACG8MG_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFQ0375956.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         4055 a         2912 c         2306 g         4073 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagccgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgttat
//
LOCUS       DAZKNH010000070        13212 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000070 DAZKNH010000000
VERSION     DAZKNH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.070"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1016
                     /locus_tag="ACG8MG_001924"
     CDS             147..1016
                     /locus_tag="ACG8MG_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0375957.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ACG8MG_001925"
     CDS             1225..2250
                     /locus_tag="ACG8MG_001925"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0375958.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ACG8MG_001926"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ACG8MG_001926"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFQ0375959.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="ACG8MG_001927"
     CDS             3333..5036
                     /locus_tag="ACG8MG_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0375960.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ACG8MG_001928"
     CDS             5038..7071
                     /locus_tag="ACG8MG_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFQ0375961.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ACG8MG_001929"
     CDS             7082..7522
                     /locus_tag="ACG8MG_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0375962.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ACG8MG_001930"
     CDS             complement(7722..8849)
                     /locus_tag="ACG8MG_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0375963.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ACG8MG_001931"
     CDS             complement(8852..9529)
                     /locus_tag="ACG8MG_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0375964.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ACG8MG_001932"
     CDS             9793..11559
                     /locus_tag="ACG8MG_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFQ0375965.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ACG8MG_001933"
     CDS             11574..12011
                     /locus_tag="ACG8MG_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0375966.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ACG8MG_001934"
     CDS             12024..13175
                     /locus_tag="ACG8MG_001934"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFQ0375967.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4489 a         2293 c         2731 g         3699 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aaacggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggggcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DAZKNH010000071        13201 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000071 DAZKNH010000000
VERSION     DAZKNH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.071"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..1907
                     /gene="yfcC"
                     /locus_tag="ACG8MG_001935"
     CDS             168..1907
                     /gene="yfcC"
                     /locus_tag="ACG8MG_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFQ0375968.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2064..3248
                     /gene="iadA"
                     /locus_tag="ACG8MG_001936"
     CDS             2064..3248
                     /gene="iadA"
                     /locus_tag="ACG8MG_001936"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFQ0375969.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3359..3562
                     /locus_tag="ACG8MG_001937"
     CDS             3359..3562
                     /locus_tag="ACG8MG_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0375970.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3663..4325)
                     /locus_tag="ACG8MG_001938"
     CDS             complement(3663..4325)
                     /locus_tag="ACG8MG_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0375971.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4338..5840)
                     /locus_tag="ACG8MG_001939"
     CDS             complement(4338..5840)
                     /locus_tag="ACG8MG_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0375972.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6388..8067
                     /locus_tag="ACG8MG_001940"
     CDS             6388..8067
                     /locus_tag="ACG8MG_001940"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0375973.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8194..8673
                     /locus_tag="ACG8MG_001941"
     CDS             8194..8673
                     /locus_tag="ACG8MG_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0375974.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8892..9572
                     /locus_tag="ACG8MG_001942"
     CDS             8892..9572
                     /locus_tag="ACG8MG_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFQ0375975.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9580..10911
                     /locus_tag="ACG8MG_001943"
     CDS             9580..10911
                     /locus_tag="ACG8MG_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFQ0375976.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10926..11888
                     /locus_tag="ACG8MG_001944"
     CDS             10926..11888
                     /locus_tag="ACG8MG_001944"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFQ0375977.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11999..12337
                     /locus_tag="ACG8MG_001945"
     CDS             11999..12337
                     /locus_tag="ACG8MG_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFQ0375978.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12416..13123
                     /locus_tag="ACG8MG_001946"
     CDS             12416..13123
                     /locus_tag="ACG8MG_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFQ0375979.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4212 a         2324 c         2891 g         3774 t
ORIGIN      
        1 atggaaagag aaaaaaataa tcatacatag gagaaaaaaa acaaaataga aaaaagtgat
       61 tttctaataa attattcatg tggaaatgtc atttttactg tataattcat ttgattatcc
      121 attttttatt catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa
      181 attcttcagg aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca
      241 ttatttttgg tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt
      301 tcagcacgca agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt
      361 tacgtcaaga ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat
      421 tagaagaact gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat
      481 tggaaaaagt cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct
      541 ccttgacaga tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa
      601 aattgcataa aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa
      661 attttgtttt tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg
      721 cattgatcct tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg
      781 caggtatcta tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat
      841 tattctttgc tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat
      901 tctcaatggt gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa
      961 ccgtgactta tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg
     1021 tagcggtggc tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga
     1081 ttatgtgggt agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa
     1141 tccgtaaaaa accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata
     1201 tcaaaaagac atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag
     1261 agatgttagt tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc
     1321 cagaaatcgc ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt
     1381 tcaaattaga tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg
     1441 atttagctgg tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag
     1501 gatctgatgc atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt
     1561 tgaccggtgt tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca
     1621 acttggttgt tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac
     1681 tggcagattt agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag
     1741 gttttatgga cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac
     1801 ggatcgaatg ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt
     1861 taggaactat tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg
     1921 taaataaaaa agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa
     1981 aatcaaagga gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat
     2041 acttaaagaa agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag
     2101 attacttagg aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac
     2161 agctagaagg cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac
     2221 taaccccggg tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt
     2281 atcaaaatcg aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag
     2341 ttgtcggttg tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag
     2401 cgaaaggtct ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc
     2461 cggtaaaaac agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg
     2521 gaatcggaga aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg
     2581 ctcgtgcagt agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca
     2641 atgtccactt aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa
     2701 cagaaattcc gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg
     2761 aagcttgcgt ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc
     2821 ctgatttttg ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga
     2881 tcgaggaaga tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc
     2941 cttattttga tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc
     3001 tagtagggat caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag
     3061 ctataaccag taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg
     3121 gtgctgatgc agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg
     3181 ctaaaggaga aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa
     3241 gtttttaatc aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat
     3301 ctgttgttga tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat
     3361 ggcagattta aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga
     3421 agcatacggc aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact
     3481 aggtgctgac atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc
     3541 tcgaaaagac gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa
     3601 tccgaaccat atctgcgaga cagatatggt tcggattttt tatttgatca ttatcctgtt
     3661 ttttaaactt cgattttttg aaatttttta ttttttagtt caaagacttc atcacagctt
     3721 tttgctactt caatctcatg agtcacgatg ataatacagc gattttgttc atgggcgatt
     3781 tcttggaaaa gtttgacgat atcttgtgaa gttgtttcat ctagatttcc agtaggctca
     3841 tccgctacga ttagttgatg ttcacagcag atagcacgaa cgatagcgac tcgctgctgc
     3901 tgacctcccg aaagtttggt cacctgtttt tttgctaata tttcatcaat tcctacacga
     3961 gccagtcgat ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt gatttccatt
     4021 gctgctatga cgttgtctag agccgataaa taaggaagca agttgtaagc ttggaaaata
     4081 atcgagacat catttttacg ataacttctt aacccgattt tgttcagtga acgatcttga
     4141 tagtatacct ttccttcttt tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt
     4201 tttccagaac cacttgctcc taaaatagcg tacatttttc cagcttcgaa attcagttct
     4261 acatctttat aaagcgcatc ttgcggatct tgataccaaa atcccaattg ttctgtccgt
     4321 aacatcaaat tcctccttta gtttaatagg atttttcttg ggtttaatct taatatccct
     4381 atagaagcta aaaagacaga aaacagactg attccaaatg ctactccggc taatagtccc
     4441 aattgtgcag gtttgacatt gatttccaat tcgctgactt gatcagaaac agcaaatgga
     4501 ttattcgtgg ttccttgttg accatcgcgt tgaccgcctt gttcttgtct gttacctggc
     4561 atttgtccac cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt
     4621 agctgctgtc ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca
     4681 agaatcatac aaatagccac ttcggtaaac aattggagaa tgattttact tctggattct
     4741 cctaaagata gcaagacgcc taactcatgc cgacgctccc gaatggtaat cataatgatc
     4801 agagtaagta tgataatacc ggctacagcc actagtaaaa caacattttt agaaaaactt
     4861 gccacgttgt ttaaaggttc caacattgac tgatacatgg aatcatttga ttgtaagcta
     4921 aatgtatctg tgtcgatcag cttctcagct tctttgagaa actcatccat tttatctgga
     4981 tcagatagag tgtaaatagc agaatctatc gtgttatcgt cagatgtccc atttagttca
     5041 tttgcaaaag tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt gaatttcata
     5101 cccatactgc cgcttgtttc tctggattca taaattcctt tgatcgtcat ttcataagtg
     5161 gtatcttcat cttcagtact cgtgattgtg aaagtatctc cgactgccag gtcatttgtt
     5221 tctgctagag tagaatcaat gactacattg tttgtgcctt tgtcgctgtc atctattcct
     5281 tcaccatccg tgatttttgc tgttccatca gaaaaattac tattttgtgc agttgctgac
     5341 acaccactga tttgaaagtc tgcttgagtc atttgcggat tcccgcccat tcctccagca
     5401 ggttcaccgc ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga actagtgatg
     5461 gcagttattc catcttttgc catagctgag gcggaacttt caaaggaata actctttaca
     5521 ttatctaatt cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg
     5581 cctggatctg gacgttcttc agtggtagaa ccgcttgaag aagtttgttc ctgtttttta
     5641 aatgaagctt ctcgattggc acttaaagtc actgttgctc caacttcttt ttgtgcttga
     5701 ttcgctgctt cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa aataagaatg
     5761 gctgtgaaca cggcaaacaa taacaatgaa cgtccccatt ttacttttgt acttttccat
     5821 gcacgtttca taaaattcat attttgcatc cctcctagta gatgcatttg ataatagaca
     5881 acctatctta aagctagctt aaatcttatg ctgtttgcac accatcaaca tgaggttctg
     5941 tatatataaa aagtcactat agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt
     6001 tcaaaccgac gaagaagaaa tgaaagagga atagcaaaag aaatcctaga tgctacagag
     6061 ggatttttac gtgcatagtt gttctaacaa ttgctgatac tagcgattta gtttatttgt
     6121 tgtataataa tggggaacag ctgaatactt atataagtat tctggctata gcgtttcgca
     6181 aaagatccat tttgttaatg aagtatatcc aaaataaaat gcccatccaa ctgtcagaac
     6241 gcggatgtgc tggtaatttt cctgctgatt ctccataagt tgagaaatag tgattttgca
     6301 tatgcttcat tggaatggct catatattta gataaagaag aaaatcgaca tctttaaact
     6361 tggatgtcat aagaagaggt gaagtttgtg agtaccatta aaattatccc attaggcggc
     6421 gttcgtgaaa atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat ttttatttta
     6481 gattgtgggc taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat
     6541 tttacgtatt tagaagaaaa cattgatcgt gtagctggtg tctttttaac acatggacat
     6601 gcagatgcga tcggggcact tccttacttg ttgtcaaaaa tttctgtacc agtattcggg
     6661 acagagttga cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc agccaaagga
     6721 tttaaggatt tccatgtggt agatgagcat acagaaatag actttggtga aacagtagtg
     6781 agctttttcc gtaccactca tacgatacca gattcagtgg ggatcaatct gaaaacaaaa
     6841 gaaggtagta tcgtttatac gggagatttt aaatttgacc aaacggctat cccaatgtat
     6901 caaacagatt atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc cttactaagt
     6961 gaatcggcaa atgcagaaaa tcctcagcct gtagcttctg aagcccaaat agcagatgaa
     7021 gtatttgata cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat
     7081 ttgcaacgag tgcaacaagt attggatgca gcagaaaaag cacaacgaaa agttgtcttg
     7141 actggtcaag atttcgaacg aattattcgc acagcgatgc gtttagaaaa acttcaatta
     7201 ccaagtgaag atctgcttgt tacgttgaaa gacatgaaaa actatgatcc agaagaactg
     7261 atcattttag aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac
     7321 ggtacacacc gtaatgtcaa gattgaagaa ggagatttgg tttatatcac tactacgcca
     7381 agcatcgcga tggaaaccat cgtggcgaaa acggaagata tcatttatcg agctggtgga
     7441 acagtgaagt tgatctctga gaatatgcgc gtttctggcc atgccaatcc taatgacttg
     7501 caattaatga tcaatttgat gaaaccaaca tattttgtgc cagttcaagg agagtatcgt
     7561 gaattagctg ctcatgcaga tttggctcat gcagtaggga tgccatacaa aaatatctat
     7621 attactggac gcggagacgt gctggaatat aagaacaagc gtatgagtgt tgcaggaact
     7681 acaagtgcag aaaatgtaat gattgatgga ttaggcatag gtgatatcgg aaacattgtt
     7741 ttacgtgatc gaaaaatctt gtcagaagac gggattttcg tagcggttgt cactatcagt
     7801 cggcgagaaa agaaaatcat ttcaaaacct caaattactt cacggggatt cgtttacgta
     7861 aaagctagcc gtgatctgat gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa
     7921 catttagaaa gcaatgattt tgagtggagc aaattaaagc aagacatccg agatcaatta
     7981 ggccgttact tatttgaaca aacaaaacgc cgcccagtga tcttgccagt cattatggaa
     8041 gctactcaac gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc tctatagagt
     8101 gctcgtcttg ctcttttttt gcgaaaaaca aataattttt gaatacagca tccctcgctt
     8161 tataataaac gatagaataa agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt
     8221 tatttagaag aacaaaagat cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa
     8281 tctttcacag aagaactaca aaaaagaggg attgatccag ctttgatatg taaaactctc
     8341 gtagtgaaag gaaacaaaac tggggtgatc atcgcattga ttcctttgaa cgatcactta
     8401 gattacaaaa aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc tggaatggat
     8461 tttgtattaa tgcatacagg atatccccac ggagcaaaca cccctattgg aataaaactc
     8521 gcacatcctg attattgttt tttagcagac agttctttaa aagaaaaaga agaagtaatt
     8581 gtgtcaagcg gtgaaatcgg gagaagcctt cggataaaag tagccgattt agaaaagatc
     8641 gtccatccta tttacgctga tttaattagt taagggacga gaagagaagc taagcagagc
     8701 agagacctca aacttgagat tatatctgct tggtacttgg tgcatcctgt attatctgtt
     8761 acaattgctt tgtacatact gtactatcaa tccaatgaaa atatatttag cagctatatt
     8821 ttttcattga tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag agaaaacgcg
     8881 aggtgtaaaa tatgaaaatt acgtatcatg gacattcctg tttaacaatt gaaatggaaa
     8941 atggccaacg cgtgatgatt gacccgttta ttacaggaaa tccattgaca gacttaactg
     9001 cagaagaagt aaagacagat tggttgctga ttactcatgg tcatagtgac cacataggag
     9061 atatgattcc tattgcaaaa gaaaatcaag ccacaattat cagtattgtt gagattgctg
     9121 attatgcgaa cagcagagga gtagattcat tcggtatgaa tatcggtgga aaacatgcgt
     9181 ttccattcgg cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat gaagtagacg
     9241 gtatcgttca gtatatgggc gagcctagcg gaattattat tcaagcagaa ggaaaaacga
     9301 tttatcatgc aggggatact gcttatttca gtgatctagg attgcttgca gaagaattcg
     9361 atattgatgt cgctttcttg ccgattggcg ataactatac gatggggcca gaagatgcag
     9421 caaaggctgc gcaaacaatt cgtgccaaga aagtagttcc aatccattac aatactttcc
     9481 ctgtcattga acaagatccc gaatcttttg tagagttact ggaagatagt gaaggaaaaa
     9541 taatgagcgt ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg
     9601 attttagaat atattgaaag tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca
     9661 aaagaattga atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc
     9721 ggactagttt caacaattca acgagtaggg accatccgta tcgaacgcaa attaaaaaaa
     9781 catattgaac gtttgacatt tggcgaggtc gtcaggatca ttgaagggga cgttttaggc
     9841 ggggcggcag gattagacaa ggtcttgaat aaattcgtca tcggcgcgat gactaaacat
     9901 gccatggaac gctatattac gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc
     9961 caaaaattag cattggaaga tggcgccgca gtattgatca caggaggatt caatacgact
    10021 gctgagattg caaagctagc tgatgatctt gaaatgccga tcttacgcac ctcttatgat
    10081 acatttacag tcgcaaccat gatcaaccga gcgttaagcg accaattgat caaaaaagat
    10141 atcatgcttg tcagtgatat ctatatgcca gctgaaaaga ctcattattt gcagcagaca
    10201 gatacgattt ctgattatca gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc
    10261 gtcaaccgcc atcatcgttt gatggggatt gttacggcaa aagatgtttt agggaaatcg
    10321 ccaaaccaga tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg
    10381 agtattgcct ctgttagcca tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt
    10441 tccgatgact tgacattgga aggcttgatt acccggcaag atgtaatgaa agcgatgcag
    10501 cttgtccagc gacaaccgca gattgcagat acgatttcgg atcagatatc tggaaatatc
    10561 catacgattg atactgaccg tgaaggaaat cgtttggaaa accctaagtt caaattcgta
    10621 gtagcaccac aaatggtcaa tggcgttgga accatttctt ttggcgtact tagcgaaatc
    10681 atttctgatg tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat cttgattgaa
    10741 caagtgaacc tacattattt gcggctgatc caattggaaa gcgaattaga cattcgtcca
    10801 cgtgtattgg aaatcggcag acgttccgct aaactggata tcgaagtgtt tattgaaaac
    10861 accatcgttt ccaaagcaat cgttgtttgt caagtaatgg aacgttctta gaaagcagga
    10921 gtgaaatgac agtacagcaa gaaatagtag atacaataaa aaaatacgat aaaatcatta
    10981 tccacaggca tttgcgccct gatcctgatg cattaggatc acaagtagga ttagccgaac
    11041 tattacggga aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc
    11101 ttcgcttttt agcagaaatg caagaagttg aagatgtctt ctaccaagga gcgctagtca
    11161 tcgtgactga tacagctaat tcacccagaa tcagtgatca aagatatcag ttggcagaaa
    11221 aactgatcaa aatcgaccat catcccaatg atgaccctta tggagatctt gtgtgggtaa
    11281 atacaaaagc aagtagctgt agtgagatga ttgcagaatt tgctttgatg tttcctgaat
    11341 ctttaaagat gaacgttaag gctgcacggt tactttatgc aggaatcgtc ggagatacag
    11401 ggcgcttttt gtatccctcc acgacgtccc gtacattaga gattgcttcg atattgagaa
    11461 attatccatt tgatgcaagt gctttgaaca gagagattga acaaatgccg atgaaagtcg
    11521 ctaaactttc cggttatctt tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa
    11581 taatccttcc gcaagaaatt cttgataaat atggaatcga tgattcagag acagcttcga
    11641 tcgtttcact tcctggagtg attgacgaag tgctggcttg ggggattttt gttcagcagc
    11701 cttcagggta ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat gaattggcta
    11761 aacgccatca tggaggaggc catccattag caagtggtgc gaatgcaaaa gatttagcag
    11821 aagtagagga aatctataaa gagatccaag atatatgcca atcgtataag agcagaaaaa
    11881 cagaatgata cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt
    11941 caagatcttc tttttccgtt acaatagtgc tcactaaaga ttttaaagga gaaagaagat
    12001 gacattacca aattgcccag aatgtggctc agagtacaca tatgaagatc gaggcttgtt
    12061 gatttgccca gaatgcggga acgaatggaa caaagaagaa caagaaactg tcatagaaga
    12121 cggattagtg gtattagatg caaatggaaa ccgtctgtta gagggcgata gcgtgacagt
    12181 gattaaagac ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa
    12241 gaacatccgt ttagtagaag gcgatcataa tatcgattgc aaggtagaag gatttggacc
    12301 gatgaaactg aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca
    12361 ttcttttcct actactaatg ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta
    12421 tggttatgga atgtatggtt ttttagatcc gacatttctt ttggtcattc ttggattagt
    12481 tatttccgga attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag
    12541 aagcaaaaac aatgtgactg gtacacaagc agcacaatat atattacaaa gtcaacagat
    12601 aaataatgtc ggtgttcaac aaattgccgg agatctcaca gataactaca attcaggtaa
    12661 taagatgttg agtttgtctc aagcaactgc acagtctact tcagttgctg cgataggtgt
    12721 agcagctcat gaatgcggac atgcggtaca agatgcttcg ggttatgtcc cattgaagat
    12781 tcgtgcagca cttgttccag cagctaactt tggttcgatg atttcgttcc ccttgattat
    12841 ggtaggtgta ttgcttagct ggaactctac gttaatcaat atcgggattt tcgctttttc
    12901 tttagcatta cttttccaat tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc
    12961 aatccaaatt ttgagtgaag gcggactact gacggaagaa gaagtaccga tggcaagaca
    13021 cgtattattt gcagcagcac tgacttatgt tgcagcagcg ctttcgacat tcctgcagct
    13081 gttgcgcttg atcttattgt tcggcggcaa ccgaagagat taaaataaag aagttgtgaa
    13141 atcagcgctt tgatctgagc taataccgag agttagctga ttgaatacca tttatttgga
    13201 a
//
LOCUS       DAZKNH010000072        13081 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000072 DAZKNH010000000
VERSION     DAZKNH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.072"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..686
                     /locus_tag="ACG8MG_001947"
                     /pseudo
     CDS             78..686
                     /locus_tag="ACG8MG_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1734
                     /locus_tag="ACG8MG_001948"
     CDS             706..1734
                     /locus_tag="ACG8MG_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0375980.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="ACG8MG_001949"
     CDS             1731..2489
                     /locus_tag="ACG8MG_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0375981.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="ACG8MG_001950"
     CDS             complement(2575..3315)
                     /locus_tag="ACG8MG_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0375982.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..4629
                     /locus_tag="ACG8MG_001951"
     CDS             3376..4629
                     /locus_tag="ACG8MG_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0375983.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4924..5178
                     /locus_tag="ACG8MG_001952"
                     /pseudo
     CDS             4924..5178
                     /locus_tag="ACG8MG_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5300..5626
                     /locus_tag="ACG8MG_001953"
     CDS             5300..5626
                     /locus_tag="ACG8MG_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0375984.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5694..6407
                     /locus_tag="ACG8MG_001954"
     CDS             5694..6407
                     /locus_tag="ACG8MG_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0375985.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6717..7569
                     /gene="licT"
                     /locus_tag="ACG8MG_001955"
                     /pseudo
     CDS             6717..7569
                     /gene="licT"
                     /locus_tag="ACG8MG_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7690..9555
                     /locus_tag="ACG8MG_001956"
     CDS             7690..9555
                     /locus_tag="ACG8MG_001956"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0375986.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9569..11012
                     /locus_tag="ACG8MG_001957"
                     /pseudo
     CDS             9569..11012
                     /locus_tag="ACG8MG_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11120..11821
                     /locus_tag="ACG8MG_001958"
     CDS             11120..11821
                     /locus_tag="ACG8MG_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0375987.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11947..12459
                     /locus_tag="ACG8MG_001959"
                     /pseudo
     CDS             11947..12459
                     /locus_tag="ACG8MG_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12857..13066)
                     /locus_tag="ACG8MG_001960"
     CDS             complement(12857..13066)
                     /locus_tag="ACG8MG_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFQ0375988.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
BASE COUNT         4390 a         2194 c         2762 g         3735 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     4501 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     4561 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     4621 gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag atagagacaa
     4681 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     4741 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     4801 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     4861 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     4921 agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag agagttagaa
     4981 caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt agagtactcg
     5041 ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt ttggggagaa
     5101 gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca tacacatggt
     5161 ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     5221 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaaatg
     5281 attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta gagatgaacg
     5341 taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt tggaagcatc
     5401 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     5461 acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg cagacaacct
     5521 tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     5581 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat gtgtcattcg
     5641 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     5701 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     5761 gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag acaggagata
     5821 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     5881 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcagatga
     5941 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     6001 cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     6061 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     6121 tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg acagatttag
     6181 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     6241 tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc gacatggacg
     6301 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata aaagatgcga
     6361 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     6421 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     6481 tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     6541 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     6601 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     6661 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     6721 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     6781 tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga gatttcgcca
     6841 gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca attccagcag
     6901 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     6961 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     7021 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     7081 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     7141 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     7201 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     7261 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     7321 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     7381 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     7441 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     7501 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     7561 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     7621 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     7681 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     7741 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     7801 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     7861 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     7921 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     7981 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     8041 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     8101 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     8161 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     8221 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     8281 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     8341 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     8401 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     8461 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     8521 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     8581 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     8641 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     8701 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     8761 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     8821 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     8881 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     8941 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     9001 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     9061 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     9121 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     9181 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     9241 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     9301 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     9361 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     9421 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     9481 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     9541 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     9601 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     9661 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     9721 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     9781 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     9841 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     9901 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     9961 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    10021 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    10081 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    10141 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    10201 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    10261 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    10321 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    10381 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    10441 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    10501 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    10561 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    10621 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    10681 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    10741 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    10801 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
    10861 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    10921 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    10981 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    11041 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    11101 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    11161 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
    11221 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    11281 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    11341 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    11401 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    11461 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    11521 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    11581 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    11641 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    11701 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    11761 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    11821 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    11881 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    11941 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    12001 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    12061 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    12121 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    12181 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    12241 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    12301 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    12361 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    12421 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    12481 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    12541 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    12601 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    12661 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    12721 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    12781 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    12841 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    12901 agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg
    12961 cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac aaaatgattt
    13021 tttcgttaat aaaagaaaca agtatttgtt gtttaaaata atacacgtta ttggcatata
    13081 g
//
LOCUS       DAZKNH010000073        12742 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000073 DAZKNH010000000
VERSION     DAZKNH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.073"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8MG_001961"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8MG_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0375989.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG8MG_001962"
     CDS             complement(323..892)
                     /locus_tag="ACG8MG_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFQ0375990.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG8MG_001963"
     CDS             1078..1980
                     /locus_tag="ACG8MG_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0375991.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8MG_001964"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8MG_001964"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFQ0375992.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8MG_001965"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8MG_001965"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFQ0375993.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG8MG_001966"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG8MG_001966"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFQ0375994.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="ACG8MG_001967"
     CDS             8554..8739
                     /locus_tag="ACG8MG_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFQ0375995.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="ACG8MG_001968"
     CDS             complement(8801..9997)
                     /locus_tag="ACG8MG_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0375996.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="ACG8MG_001969"
     CDS             complement(10167..11603)
                     /locus_tag="ACG8MG_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0375997.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="ACG8MG_001970"
     CDS             11896..12705
                     /locus_tag="ACG8MG_001970"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0375998.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4013 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aa
//
LOCUS       DAZKNH010000074        12492 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000074 DAZKNH010000000
VERSION     DAZKNH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.074"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACG8MG_001971"
     CDS             <1..375
                     /locus_tag="ACG8MG_001971"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0375999.1"
                     /translation="VKDNRNSIRKEVLHLIKFVKKTVAIPSEKKINESTRKLYRRANK
                     KALKLSAFLSSLSLKLSLILFISEHVSITDFLNLAKNPYLKGVRSILWQGLQSLACLE
                     VRTWFSYSSCRKIFLMMILSIN"
     gene            complement(324..893)
                     /locus_tag="ACG8MG_001972"
     CDS             complement(324..893)
                     /locus_tag="ACG8MG_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0376000.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="ACG8MG_001973"
     CDS             complement(967..2256)
                     /locus_tag="ACG8MG_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376001.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2514..3458
                     /locus_tag="ACG8MG_001974"
     CDS             2514..3458
                     /locus_tag="ACG8MG_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0376002.1"
                     /translation="MEEKIEESKQKIDQLIQELEKAVIGKRSTIQLMIVALLAGGHVL
                     FEDIPGVGKTLLVKALAKAVQGTFSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPI
                     FTTILLADEINRTTPRTQSALLEAMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTY
                     PLPEAQLDRFLFRLQIGYPSFDDELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANV
                     FVSPEVASYALQLVSATRSHAAIQLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQD
                     LVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQIPVPVR"
     gene            3472..4545
                     /locus_tag="ACG8MG_001975"
     CDS             3472..4545
                     /locus_tag="ACG8MG_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFQ0376003.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="ACG8MG_001976"
     CDS             4535..6706
                     /locus_tag="ACG8MG_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFQ0376004.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACG8MG_001977"
     CDS             complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACG8MG_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFQ0376005.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9009..11195)
                     /locus_tag="ACG8MG_001978"
     CDS             complement(9009..11195)
                     /locus_tag="ACG8MG_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0376006.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACG8MG_001979"
     CDS             complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACG8MG_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFQ0376007.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11417..11857)
                     /locus_tag="ACG8MG_001980"
     CDS             complement(11417..11857)
                     /locus_tag="ACG8MG_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFQ0376008.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11931..12429)
                     /locus_tag="ACG8MG_001981"
                     /pseudo
     CDS             complement(11931..12429)
                     /locus_tag="ACG8MG_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
BASE COUNT         3520 a         2630 c         2217 g         4125 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt tttactccat
     9001 cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa tacactgacg
     9061 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     9121 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     9181 gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact catcagcgtc
     9241 acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc aacatcagct
     9301 aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     9361 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     9421 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     9481 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt ggcttctagt
     9541 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     9601 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
     9661 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
     9721 ctgattccag cacctgggtg agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
     9781 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
     9841 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
     9901 gtaatcgtcc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
     9961 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    10021 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    10081 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    10141 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    10201 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    10261 ccgacttgtg tgacttccat gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc
    10321 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    10381 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    10441 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    10501 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    10561 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    10621 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    10681 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcga cttgattgca
    10741 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    10801 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    10861 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    10921 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    10981 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    11041 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    11101 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    11161 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    11221 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    11281 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    11341 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    11401 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    11461 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    11521 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    11581 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    11641 gatgataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    11701 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    11761 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    11821 aatcactaat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    11881 aatgtaatca ttaacttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    11941 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    12001 atcagtttat ttcttgcatt agctacttgg atcagctgct gtatttttgg ttcattgttt
    12061 ccttcaaaat gccagataaa agataaaaat tctaagtatt tcctgtttag tttctccgaa
    12121 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    12181 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    12241 ttaatccttt cctctaacgt agaagtataa tttccttcaa cgatccatga cggattagat
    12301 accataaacg attgctgttc ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt
    12361 gatttctttg atcttccttg cgagggacga ttttcccgac ccactttgtc caatcacgat
    12421 gatcttcatt cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac
    12481 gccgcaaaaa aa
//
LOCUS       DAZKNH010000075        12432 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000075 DAZKNH010000000
VERSION     DAZKNH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.075"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..191)
                     /locus_tag="ACG8MG_001982"
     CDS             complement(<1..191)
                     /locus_tag="ACG8MG_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376009.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(201..1016)
                     /locus_tag="ACG8MG_001983"
     CDS             complement(201..1016)
                     /locus_tag="ACG8MG_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0376010.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1278..1721)
                     /locus_tag="ACG8MG_001984"
     CDS             complement(1278..1721)
                     /locus_tag="ACG8MG_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFQ0376011.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1855..2193)
                     /locus_tag="ACG8MG_001985"
     CDS             complement(1855..2193)
                     /locus_tag="ACG8MG_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0376012.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2181..2558)
                     /locus_tag="ACG8MG_001986"
     CDS             complement(2181..2558)
                     /locus_tag="ACG8MG_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0376013.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2782..3975
                     /locus_tag="ACG8MG_001987"
     CDS             2782..3975
                     /locus_tag="ACG8MG_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0376014.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4141..4563)
                     /locus_tag="ACG8MG_001988"
     CDS             complement(4141..4563)
                     /locus_tag="ACG8MG_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0376015.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4958..5152
                     /locus_tag="ACG8MG_001989"
     CDS             4958..5152
                     /locus_tag="ACG8MG_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376016.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5149..5595
                     /locus_tag="ACG8MG_001990"
     CDS             5149..5595
                     /locus_tag="ACG8MG_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376017.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(5805..6977)
                     /locus_tag="ACG8MG_001991"
     CDS             complement(5805..6977)
                     /locus_tag="ACG8MG_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0376018.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7183..7998)
                     /locus_tag="ACG8MG_001992"
     CDS             complement(7183..7998)
                     /locus_tag="ACG8MG_001992"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0376019.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8148..8594)
                     /locus_tag="ACG8MG_001993"
     CDS             complement(8148..8594)
                     /locus_tag="ACG8MG_001993"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0376020.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8591..9106)
                     /locus_tag="ACG8MG_001994"
     CDS             complement(8591..9106)
                     /locus_tag="ACG8MG_001994"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0376021.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(9179..10093)
                     /locus_tag="ACG8MG_001995"
                     /pseudo
     CDS             complement(9179..10093)
                     /locus_tag="ACG8MG_001995"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(10090..12417)
                     /locus_tag="ACG8MG_001996"
     CDS             complement(10090..12417)
                     /locus_tag="ACG8MG_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFQ0376022.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         3513 a         2430 c         2272 g         4217 t
ORIGIN      
        1 atgcaacctg gaccaataaa aagtaaagaa aaaataaagg aaaagacatt ccttccatct
       61 atcgacaaaa ggaacgcagc tgctatatac aagatattga cacaccaaaa acaaaggcta
      121 cttgccaatg attgatacaa ccaataaaag aattccatat aaacattttt gtcaaaacgc
      181 tgctctttca taatcacctc ctattccttc attcctgatg aagcgatccc ttctacaaaa
      241 tatttttgca tcgctaaaaa tataaaagca acaggaagaa cagaaacaac tgaggcagcc
      301 atgattaatg cgtactctgc atcaaatagc ccaataaaca ttttcaaccc taactggatt
      361 gttttatttt ccgtagaaga caaataaatg aatggcccca tataatcgtt ccatgtatta
      421 acgaatgtga tgatcgtcaa actggcaatc gccggttttg taagaggtaa aatgatcttg
      481 cgatagattc cccactctga taagccatcg atccgcgcac tctcacataa agaatctggg
      541 atactactgt agtattgctt caacaagaac acaccaaaag cattgaacgc ttgtaatagc
      601 accaacgacc acaacgtata tgttaaaccc acttttctca tcataatgaa ctgcgggatc
      661 atataagact gccaagggac agcaatcgtg ccgatatatg ctaaaaataa aatatcgcgc
      721 cctttaaaat ttagtttaga aaacccgtaa gcagcaaaac tagaagtaaa caactgaatc
      781 aacgtgatca caacgcttaa aaaagctgta ttcttaaaaa atgtcactaa aggaatacga
      841 gaccaaataa ccagataatt ctctaaatgc caagttttag ggatccattg aatcggaatc
      901 gtgaacactt cattatttgc tttgaatgaa gcagaaacca tccataaaaa aggaatgatc
      961 gtcaataaag ctaatacaat cagcagaatc atacccaacc attgaataaa gggcatgttt
     1021 tttgaacgaa tttttgcttt ttttctttca tttcgaacac ctccaaagtt ataatcttca
     1081 tttctttgta atgattacaa tccagaacaa cggttattat agctgattca tttccgttct
     1141 tcttatgttt gtaagcgctt atttaacaag ttatgtaaat ctctttattt tttttgattt
     1201 aaaaatgaaa aaatacaaaa gcaagactgt catacgtctc gcttttgtat tgaaatttct
     1261 tcgattagag atacttatta ctgttcaaaa gcttctttga aacggtgaat caattcattg
     1321 actacaggtg ataaaatccg attctttttc cagactagca tgcagttcgt ttcaatcatt
     1381 gggtcaagcg gaacgaatgt cactcgcttt gaaagatgac ttacagcacc ttctatgact
     1441 aaagcagcac ctacctgatt ctcaattagc gcaaatacat tgaaaatcaa attgtacgtt
     1501 cctgtgatca gcaactgctc cttttttatg cctaaccagt cagcaaacat tcgctgtatt
     1561 tctggacgct ttgagatcaa aagaggtaca ttcgcaaagt cttcttttgt tatcacgtct
     1621 ttttttgcta aaacagaatt ttctaaaatg agcaatcccc atttttcctt tcttggtaac
     1681 aaaatcgttt cgtatttctc tgtatctact ggatctagca aaatcgctaa atctaacagg
     1741 cttttttcta gtcactccgt gatatcatca cttgttccgc tgtggatatg aaattgaacg
     1801 ctgggataat cacggatcaa cttttctagt aatagtgcca atgtattcga attatcagct
     1861 tccacacagc cgatcgtaaa tcggccgctg aataattgtt tttttctgtt ctcgaattct
     1921 tgagtggttt gattcgttaa atctaaaatt tcttctgctc ggctttttta gaaaataacc
     1981 tgcttctgtc aagatcattt tgcggttttc gcgtacaaac agttctgttc ctagttcctc
     2041 ttctaactcc ttcagttgtc ggcttaaggt tggttgtgta agatgtagaa cttctgcggc
     2101 ttgagagatt gttccttctt ctgcaattgt ccaaaaatat tttagtaagc gtaactccat
     2161 agcgatgctc cttgattttt ttacttgata cattccattt tatttctaga taacacacga
     2221 tcatatttct tgatttttcc ttgtaaacgt tccaaacact cttgcatttc agcaatttct
     2281 ttcgtcaaaa gttcactttc ttcaatcaaa agcttacgcc gctcaaatac agttgcttct
     2341 ccttcgctag ataggcgaat atattcaatc aaagactcga tcgataatcc ggcatttcgc
     2401 atacagatcg taaagtcgac ccattttagg tcttcttctg tataatcacg gattccgcct
     2461 tttgtccgat taactggtgg aatcaacccg attcgttcat aatagcgcaa tgtatcggcc
     2521 gaaatgccct tttcttcact gacttgtttg atattcatct gatcactctt tctcttaatt
     2581 tcttgccact ttacttatgt attttattta ttctacaact ttaagtgtac tccaagtcaa
     2641 tactcatttc tattaaaact aagctggatt atctatatcg gttgtttgtg tattatttca
     2701 aataaaaaag tcttgttttt ttattctttt ttggatatac ttttaacaat ttagggaaat
     2761 tttaggaaaa ggggtataaa aatgacaacg tatcagcgaa aggtattatt atcgacatcg
     2821 gcgggaattg ctttagagaa tatggatatt atgtttttag ctttttcatt atcttcaatg
     2881 attacttctt ttcatattag tggtacgcaa gcgggtttca tttcaacgat cactaatttg
     2941 gggatgctca ttggaggtat tttttttggc atcatagctg ataaatatgg acgtgtgaaa
     3001 gtattttctc aaacggtcct tctgttttcg attgcttctt tattgatgta ttttgcttca
     3061 aacatctact tggtttatct ctttcgcttt atcgcaggtg taggagcggg aggagaatac
     3121 ggggcgtgta tgtccttgat ttccgaaagt ttttcaaaaa aacaaattgg acgagcttct
     3181 tcggttgctg gtattggtgc gcaggtcggt gcggctttgg ctgcgatttt agctgctgtg
     3241 atcattccaa cgtttggttg gaagatgttg tatgtcgtag gtgtactgcc ggtgttgatg
     3301 gtcttgttca ttcgtcgcgg cttgactgaa ccaaaggaat tcaaatctgt acaatctgcc
     3361 gaaaagaaaa ccaaacttag cagactgttt gaatcaaaac atcttgcttg gcaaacatgt
     3421 ggccttagta tcatggtaac tgtccaaatt gccggctatt ttgggttaat gaattggctg
     3481 ccttcaatta tgcaaaatcg attagggctt tcggtttctg ggtcttcatt gtggatggtc
     3541 agtactattt taggcatgtc tttggggatg atgacttttg gcataatcat ggataaattg
     3601 gggcctaaac tagccttttc tttgttttta atttgttcat ccttatctgt ctatctactt
     3661 gtgttagccc atagccagtg gagtttggta gcagccgctg tgatagtcgg ctactttatc
     3721 aatggcatgt atggtggata tggcgcagtg attagtagtt tgtatccaac agaaatccgc
     3781 gcgacggcga ataatttcat tatgaacctt ggacgagctg ttggtggttt ttcttctatc
     3841 gtcatcggtt tcttgatgga tcattatagt ttaactgccg taatcttgtt tttgagcggt
     3901 atttacctag tcagtctgct ggtcttgaca actcttactg gtgttacagc attaagggaa
     3961 agcttgcagc attgatagtt gagtctgtaa atgacaagaa agaggtgtgg caaaagcttg
     4021 catctgcggt aataagacca gccaaatcaa aaacatgaga cactgtttcc gtttggctgg
     4081 tcgtattact tgatgcaggg cagctttttc aactgctaat ttccttctat ctggcggcga
     4141 ctattctttg acgtccgtca tttcaaagta ctttactaat aacttagcat aacgaaaaac
     4201 tgtggataca ctcgtatatt gagcgtctgc ataatcttcc aaatttttct caggatgttg
     4261 atacatgaat tcaatcaaat gataattata tgattttctg tagaaatgaa gataaataat
     4321 gtagattggg aagttctcct gcttttccat agaaaagcgc cgattattct cattcttcaa
     4381 aatgacctta tcaggatatt tttggaacaa ttcttctaaa tctgttagat aattcaagat
     4441 agacttactt gataccttca attcgttttg tagttcttct attgaacacc aacgttctgt
     4501 ttcaagcagt aaatgaatca ttttaatttt cttttgagaa acttttgtta aaaaagtctg
     4561 catattttct cctcctgtat ctactttact atactacata caagaaaaaa aacggataca
     4621 aatatcatat tgtcacaccg tcaatagtgc ataaacctgt tattatttct ctttttttcc
     4681 taagtattct attgatatga atcatttaat cgtttattat ataaatgtca ataatctaat
     4741 aataaaataa cgtacatatt ttattattgc atacttaata acagaactat agcaaaaaat
     4801 cccgtgtatc aacacgggat tagattaagt ttcctcaatc actattcata acacaattat
     4861 tataacaagt aagtaaacaa ttgaaaagga catttattga aaacggttgt tttttactta
     4921 aggaggaaat ttagcgaaaa aattagttgc attgtttttg gtcgcaggaa gtttgttgag
     4981 tgggtgtggt gctactacgt ctactactca aagcacaagt tcagatcagg cttccagcag
     5041 cagtaccacg atctccgaaa gtacggcaga ttcatcgaaa gaacaagaac aaattgaaaa
     5101 gaaaaaagaa gagaaacaaa aagcggaaaa agctgcacta gaaaaaaagt gacagaagcg
     5161 gaatctgctt tgtcacaagc tgaagcaaat cctacaaaag aaaactatgc aactgcttca
     5221 gcagctatcc aagcgctacc tgaaaacaaa caagagttga caagccgtct tgctgcaatt
     5281 gattccacaa tcaaagcaaa agaagcagaa gaagaacgga aaaagcaaga agaaattgca
     5341 cgacaacaag cagaacaagc gcaacaagaa gcaatcgcac agcaacaagc agaacaggcg
     5401 aggcaagaag caatcgcaca gcaacaagca gaacaggcgc gacagcaatc agcagctgaa
     5461 caggcctctc agcaggcaca agccgcagct cctgagcaaa ataatgaaca aactgtctat
     5521 gtcacaccga ccggttcaaa ataccataca cataaatgtg gaaatggtac gtatacccct
     5581 gccactttaa gctaagctca agctcgtggt ttaacccctt gctccaaatg ttatggaaac
     5641 taattcaaaa gttatactat aataataaaa ttcttctgaa acataaaaaa atagaagatg
     5701 gcgttccaga tagtaaacca aaaataacag tttacacgtc catttcgtca gcttctctct
     5761 tttttcattt agtctgttcc ctctgctttt ttggacatct tcattcattc tttctcaatc
     5821 aaatagtaca cctctttttc ttttcgcagt ttatatcttc cttttttcaa tttcattgaa
     5881 aactgtttcg aatgtttatg ttttacatgg attttaagca ggagctcctc atgttgcaag
     5941 cgatagttcc tgccttttaa atcagaaaat ctcactagtc cgtattcatc agaagctttc
     6001 acaaatggaa ctttttctct agtaatatag tgtttgccgt tgagcgtttt tacagaaaag
     6061 accattcctt cgattggtcg tccgtttgtg ttctttacat aaaaagataa aggatagttt
     6121 cgcttagcga tggccaaaga atctaaataa gcgatgagta gccaaactaa gccgattgat
     6181 ccaagtagta taccgataaa ccaaataaaa aatttccatt ttttccattg atcaattcct
     6241 tggatcgctt tttttgcctc ttttgcttga taaggaatac gatgccccgt cacaagcagt
     6301 cgatgtgtat tgaccatata aggagtacag gtaagcaaag tcacgagatc tctgcctttt
     6361 tggataccca aagaatctac ttcatcaggt aagaccactt ggattttttc tacttgatac
     6421 gccaatgtct ttccattgat ctggatgaaa aactggtctc cttttttcaa cttaggcaaa
     6481 tctgtaaata attttgcttc tgggagtcca cgatgcccag aaagaacggc atgggtgttt
     6541 ttcccgccag tcggataaga actttcttcc aataatgacg ttcctttttg caaaaatatt
     6601 tccgttgtct gatcgaatat aggtaagctg acattgattt ttgggatttc gatgactcca
     6661 agcatatgtt ccataaaaaa ggcattacta ggtaaatctt ttaatgcttt tgcatcaact
     6721 gcttggttga aactgcctat ccctggtgat acgttttgct ctgccaactg ttggtttttc
     6781 tttgtcattt tttcctgctg tttttttatt tcctctgaat tcttattcga tgcttgcttt
     6841 tgataatgga tgatcatttg ctgggcaaga aaatcattca gtgaatcttc tacaaaagga
     6901 taagaaaaga tcccgatccc aacgactaaa atgattgcca tgaagatgtc cagtattctt
     6961 attctgcttt tattcatcat gtccacctat tttctaatat gtatatgaag aggttgtgaa
     7021 atcagcgcct tgacctgagt attagcgaag caaacggcaa aatagttcct cctattttgc
     7081 cgtttgttga gcgaatacca agagaccgac cacttgtcac aacctttctc cccatttgaa
     7141 tcactatcgt ttatttatcg tatgattttt gtttccaata gtttaagctt ctgtttcttg
     7201 atggcgtttt ttgaaataga tcactgctcc tccaactaaa gcagtaccta ccaagatgaa
     7261 tacgatgatc cccataccac cagttgaagg caagattcct ttaggttggt tatcgatcgt
     7321 tgtttcatca gaagttgttg gatcatcatc tactgtaaat tcagtgtacg cagcttcatt
     7381 cttaggtaat gcgtagcctt ctggtgcttt tgtttcttga aggcagtatg ccccttgttt
     7441 cagcccttcg attgaaaatt tcccttctga atcagaaatc aatttcactt cttcccagtc
     7501 agaacctgtg acttcatctg accaagcata tgggtattct cctgaggcaa ggtttgctat
     7561 ttttttcatt aatttaacag ttggatcttt tgctcttaga atagaaaatt ctgcatcggc
     7621 taatttttct tgcgtgttca catcttgttt gatgaagtgg aaatcaccgt agttttggac
     7681 attttctaca gtaatcgtgt tatccgtagt tgtattttcc cgattgacta caccgtttgg
     7741 attcccaaga ttggcttcat ctgcgccatg gatgataccg gttgtttgtt gcaattttcc
     7801 gttttttact ataaaagtag tttctttttg tggattggtt aattgataac catctggtgc
     7861 ttttgtttca actaaagtat aattgccgtc tattaatcca ttgacactga tggatccttc
     7921 agtatcagtt gtgaatgtca acgctgactc agcaggatca tagagagcga caccagtgtt
     7981 ttcatcaaaa ccggtcacga attgattctc tgaatttttg atctggaatt gcgcccctgc
     8041 taacttatct acccctgaaa agttcccagc tttctggatt ttgatattgc tttctttgat
     8101 gacattttta gcatataggt gaatcgtgtc taaaggtttg tcagggtcta gggtaagctt
     8161 aacacgatat ttggcgacgt ttcgacaccg ggctttggtg tttctacaaa gacgtataca
     8221 gcatctttcc catcagattt acctggaaga gtcaggttag ctaatccttc accagaagtc
     8281 gttacacttg cgaagggtgt agcgtctgct ggaactgctg tgtctttact tggatagtac
     8341 gtagcagtga tgtcatatac gttgaattca acgccgttca acggttcgct attcggaaat
     8401 gtcaattccg ttccgttgtt tatttgattt tctggtgctg aagtgaattt caatttatgc
     8461 aaattaaccg taacattttc tggtgtaggt gatacggctg cagaaacctc tgcagttcct
     8521 aggccgagaa cgaaaggaag cagcatgata gcagttccta aaaacgtcca tatttttgtt
     8581 ttgatgtttt tcattttatt tccctcctat tgtttccttt ttgttgtttt ttataaaacc
     8641 aagctgtccc tgttgaaagg actagcaata accctaaaat tgagaaacct ccaagcatct
     8701 cttcgcttgt ttttggaaag cttcctccat ttttttcatt tgatggacca tttgtctgca
     8761 tctgaactgg cgtttttcca ttattcgtcg gatttgcttt tggttcgttg atgatttcca
     8821 atggtatagg atgaagcgga tcataactgc ctttttcaac ttcaaagaga atggttgatt
     8881 gactcaatat atatccttta ggcggtttaa tctccactag ttcgtaactt ccaaaagaca
     8941 atccatggac agaaaatgtt ccatctttac tggaagtaaa aatcgttgct cttgaatcat
     9001 ctttggctac ccaatgattt atcccgtttt cttgcgctaa gtattggtca ttttttttga
     9061 ctagaaattg agcatctgct agtttttgtt tcgtttgccg atccactttg acaaattgtt
     9121 tgcctcctgt ttggatggca agatgtgttt ctactgtttg attgtcagtc gtcaaacgta
     9181 catcgttatc aaatgtcgtt ttttcactgt tttctttcaa tgtcatttgg taacggatcg
     9241 ttaatttttt tccagcaaac gaactaagat cttttccaga atctgataag acaaaaccat
     9301 gttttatttt ttgtgtgtga aatgtatgaa tcttgtctcc accaatcaaa aaatcgatcg
     9361 tttctggttc tagccataac tgaggatcag ctgtatcttt gatcgtatag gttcctatct
     9421 cgttgatgtt ctcaggaatt gttacggtga tgagataagg gactttatcc ccatctgcaa
     9481 aatctgtctg cttattctct actgtttttg ttaatgtgag tgcagcaggt attttttgtt
     9541 cgctctttgg atacaagtgg atggtcgtca atttatgatt agatgaatct gtcacaggaa
     9601 gcacaacgac aaatggtgct gatttttctt ttacttaatc tgaagtcttt atttccgtaa
     9661 atcggtaaac agcgtcacga ccttgctcat ctttgtccga tgcactaaaa gaagcaatgc
     9721 cctcttcctc gtctactgtt tttgtcacat tttctgccag tattttttca ctatcacttt
     9781 tttgtgctag ttttctttgt gcgtcttcca cactacttcc ttctgaccgc agcttgtaaa
     9841 attcatccga gacatcatat atagcgaaag taacgccgtt caaccctttt actccttgaa
     9901 agttatccgg gatatttatt ccgtcgtttt gactgatact tggcatctta tcgctttcga
     9961 acattcgatt atgcaaagta aatgtaatag gtgattctct agctgacaca gtcggagaac
    10021 ctataaaaaa gaccagtagg ataaaaaaga gaacagcaaa tatatccaca cgtacatttt
    10081 ttgttttttt catcgctgtt cacctgcttt tttcttacga tggataaaat aaaagaagcc
    10141 agaaaaaata aagatcagtc ctaaaaagac aaagggcagt agacctatcc cacctgtcga
    10201 aggaagcaag agagtcggat tgatcgtcaa cttagcggta tctgttgcga taatcgtata
    10261 attattgttt acacttcctt cgcctaaact aatcgttact atttgtgatc caatatctgt
    10321 ccggctgtta actgttaggg tataatccaa tgattcgcct aagacaacac cggtaacgtg
    10381 gagatttcca accgaaatcg agtgactttg ggcatcataa ttaaaggcag cgtcatcgac
    10441 agtaatcact gcgttagctt tatagatggt ataagttcct gatgtgttgg ctatatcagt
    10501 aaatgacaac ttgggatttg ctgctttgac tttgtctatt ccgctattcg ttagtttaat
    10561 tgtatagctg ccagcattaa ccatattttt tgctgattgt gcatctgctg tgtaatccat
    10621 actgctaagt gccacttgta ctggagtaag cgaactagtg acaccctctg ctagcttgac
    10681 atggattgac gggacacctg cttttccatc atatgtctta ctgccactga gctctaccgc
    10741 accgctgatt tgcttaatcg tatagacatc agatgtctgc acgatagtaa ataaatagtt
    10801 tgttctggca gctagtttag ctagtccgct ttttgtcaat tgccaagacg tataggttcc
    10861 aatttcagta ggcgcagtta cttcaactcc cttttcattc atcgctttga aatcagttgg
    10921 attccatgtg atttgatcaa tactgagatc attgtttaaa agggatgttg attctgtttt
    10981 atcattaaag gcatatttaa cgatcggtac aacagaaaga ccatcatagt acttactgcc
    11041 atttgcattg attgatactg ttgcatttgt cttataatat aggatgactt cagaaatctg
    11101 ttttccattt gcgtccttta cgattacatt tccatttgta tctaaactag ctaaaacact
    11161 cattgctgat tggttctttg tttctatcgc tgaaacagct gtaaaggtat atccgttgat
    11221 agtaggcgta ttagtcgtca ggtcttgtgc agcacttccg taagtaagat tcattatttt
    11281 agaaggagca atcatctgtc caaaatagtc ttgatactta atagtgattg gtgtttgttt
    11341 gcctgtatag gtcaccgtat cgacgttact ttctagggct gtgaaagaac cgtttgtttg
    11401 tgcaaaagaa tagcccgtta ctgctgttgg agtgataacc actttgtcac ccacatggat
    11461 tccagataat gtagtcccgt acgttccgat ttttgctgct ccaccattga ctgtataaga
    11521 aaaagttcct gtccgattcg cattgacctg aaccgttgct gcttgatagg ttggaaaatt
    11581 tgaaacattt cccttaccat caacgatctg cccatttttg atcgagtacc ccgtgaatgg
    11641 aatatcattt gctaactgta taccgattga aaaagtactc atacctcctc cggcaggaag
    11701 ccctccccat gttgagggtg taaaagaata acttttatcc gcattgggtg taactgaatc
    11761 gatcgtattt ccagcggtag ccacccccat atagatactt tttgggaatg tgatagtagt
    11821 accatttact gtggagccaa taaacagatt atggatatct ggacggttat caataaaagc
    11881 gattgctttg gttacgcctt gtaacgcagt tttatccgca ctcatcgatc cgttaaccgc
    11941 aacaatgact ccatctttgg catatatcac tgtatatcca tctggtatgg tatgtccgtt
    12001 gaaattcgca gtggccgcaa cagtactaaa tgtctggctt ctacctgttt ctgaataagc
    12061 tttctcccct gctgcttttg ctgctgtgat ttctttttct gtcggatcaa tcagttgagt
    12121 cgaggatgtg gttgaggttg tctcgttgtt gctagactga ttggtgcttt ctatggactg
    12181 tatttcagaa tagggtggac tatttgtctc ctctgtcgtt tggttagcaa aatttgacgt
    12241 ttctgtataa acgtttacat aaatacttga taagctccct aagaaagtaa aaatcaagat
    12301 gatcgaaatc ataatgggat gtaagtattt tcttttagat acagctttga gtctagaaac
    12361 aaaatgtttt tgacatatac gttggatttt attttgagtt ttcttaatca ttttcatccc
    12421 tcctctcaat aa
//
LOCUS       DAZKNH010000076        12337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000076 DAZKNH010000000
VERSION     DAZKNH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.076"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..154
                     /locus_tag="ACG8MG_001997"
     CDS             <1..154
                     /locus_tag="ACG8MG_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0376023.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(208..486)
                     /locus_tag="ACG8MG_001998"
     CDS             complement(208..486)
                     /locus_tag="ACG8MG_001998"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376024.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(601..1218)
                     /locus_tag="ACG8MG_001999"
     CDS             complement(601..1218)
                     /locus_tag="ACG8MG_001999"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376025.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1613..2437)
                     /locus_tag="ACG8MG_002000"
     CDS             complement(1613..2437)
                     /locus_tag="ACG8MG_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0376026.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2780..3196
                     /locus_tag="ACG8MG_002001"
     CDS             2780..3196
                     /locus_tag="ACG8MG_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0376027.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="ACG8MG_002002"
     CDS             3193..3663
                     /locus_tag="ACG8MG_002002"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0376028.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="ACG8MG_002003"
     CDS             3677..4450
                     /locus_tag="ACG8MG_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0376029.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="ACG8MG_002004"
     CDS             4464..5276
                     /locus_tag="ACG8MG_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0376030.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="ACG8MG_002005"
     CDS             5330..6295
                     /locus_tag="ACG8MG_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0376031.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6431..9154
                     /locus_tag="ACG8MG_002006"
     CDS             6431..9154
                     /locus_tag="ACG8MG_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0376032.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9167..9799
                     /locus_tag="ACG8MG_002007"
     CDS             9167..9799
                     /locus_tag="ACG8MG_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0376033.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10007..10231
                     /locus_tag="ACG8MG_002008"
     CDS             10007..10231
                     /locus_tag="ACG8MG_002008"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFQ0376034.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10333..10566
                     /gene="relB"
                     /locus_tag="ACG8MG_002009"
     CDS             10333..10566
                     /gene="relB"
                     /locus_tag="ACG8MG_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFQ0376035.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10556..10825
                     /locus_tag="ACG8MG_002010"
     CDS             10556..10825
                     /locus_tag="ACG8MG_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0376036.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(11004..12305)
                     /locus_tag="ACG8MG_002011"
     CDS             complement(11004..12305)
                     /locus_tag="ACG8MG_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0376037.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4030 a         1998 c         2422 g         3887 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcag atgcagcgat taaatgacta tctttatcaa ctaacattgc ttttgaaatc
     6481 tacgccttca gataattatt cagctcgcag tatttcgcaa gctctacagt tgaatagaag
     6541 tacaatcagt agctacttaa atgaaggtgt tcgtgaaggg cttttcataa aagtaaaaag
     6601 ttacccggtt ttatttttac atagaacagc tcttgaagaa ctacatatca cactgaataa
     6661 ctccgaaata gaatcaattg aaagcttgtt aacaatttct caaaaacccg cattagatca
     6721 agtaattggc tctaaaggaa gtttgaaaga agcaattgat caaattaaaa cggcagtctt
     6781 atatcctgga aaagggttgc ctttattatt gatcggcgct agtggatcag ggaaaacctt
     6841 tctagcgaat aaaatatatg aatatgcagt agaagaaaag gtcattcaca catcggcacc
     6901 tttcatagat tataattgtg cacaatatgt gagcaatcca gagttactat cctctgcttt
     6961 atttggctac acgaaaggag catttaccgg agcaagccag gagcatactg gtttgctaga
     7021 aaaagccgat ggaggagtat tgtttttaga tgaagtgcat cgcttatctg aagaagggca
     7081 agagaaatta tttacattta tggatacggg tgaattctct cctatggggg ataatagtat
     7141 tcggaaaaaa gccgatgttc gattagtctt tgcaacaaca gaaaatatat atacgacttt
     7201 cttaccaact tttcttagga gattgccagt tattgttaat ctacctagat ttcaacaacg
     7261 tccgtcgttt gaacgtctgt cattgattga tgaatttttt gtttctgaga gccaaatttt
     7321 agctaaggaa ctatctgttt ctgatgcatt gattcatttc ttaatgaata gtgattttga
     7381 aggtaacgtg gggaatatta aaaatattat caagtatgct tgcggaaacg catatatcca
     7441 ccagaagagt cagcaaacaa ttcaagttaa attattggat atgcctttag agtacagtta
     7501 taaattcaag gagcaactaa ataaacccag aaagaaaaga gcagatcgaa tctactatcc
     7561 agaagaaaca cgacaagtgc aactagaaag aaacaatcag ctttttgttc aattttttga
     7621 ccaattactt actggattct ctgaagtgat tgaaaataac aaggatatta aactattttt
     7681 agaagaaatg gttaccaagg taacacaagt aatggatacg cttatttttc aagaggatta
     7741 cgaaaaagag cagtctttat acgccattct aaactatcat attcgtcaaa gtattgattt
     7801 tatgcgagaa agctacgggt ttgaacaaga tggtaaccgg gtagtcgctt tggccaacta
     7861 tctatatgtc aaggataatc aagtgatttt tgaaaatagt cttcaagaaa agaaaaccaa
     7921 agatcagctt aacgaatttt ttgctaatgt tcttgaaacc ccttattggt atgcaaaaaa
     7981 aatcttaagc cttttatctg agtgtttaga tcaacctgtg tatgaagagg acattttctt
     8041 tattacgcta tatttttata gtttgaatgt cacgaatgtt aaaacaggta ttaagagtat
     8101 tgtgctagct catggttatt ctacagctag tagtatggcg aatgttgcca accgtatgct
     8161 gaataagaac gtttttcaag cttatgatat gcctttagat attacattag agaaaattga
     8221 agcaaaattg attcaattcc ttggagatta tcgaacagat tcaggcttga ttttattggt
     8281 agatatgggt tcactaaacc aactaggcgg tttattagtg aatcatctaa atggaccact
     8341 gttaatcatt gatcatatca atacaccgtt attattggaa gtcggccaac aagtattaaa
     8401 caataaaagt atcaccgaaa taggagcaga aattgaaaca agtcttactt tcaaaaagca
     8461 gttactactt cccaagaaaa agaaaaagaa agccatcatt acttgttgtt atacagggat
     8521 tggtagtgcg acccagattc aggagatttt aacgaaatgc ttgaaagatg cagttagaga
     8581 attagtaatt attccgtatg attacaaaaa gctgttagaa aacaagaatt ttgagactcc
     8641 ttttcaatta tacgatgtca tcatgattgt aggtacagaa gatcccaaaa ttaatcagat
     8701 tccatatatt ggattagatc gcctgattaa tgggggaagc attggcgaat ttgttcatta
     8761 cttggaaaaa gagctgacta ttgaagtgaa cgatttacaa aaagaattaa ttttcaattt
     8821 ttcaattagt aaaattgtcg aaaatttaac cattttggat ccagataagg tgctatcttt
     8881 tgtacaaaaa gcaattaaag aattatcgaa gttattaaat tttgattttt ctaataatca
     8941 gctgtttttg ttgtaccttc actgtagttg tatgattgaa cgaattgtac gtaaagaaag
     9001 tgtggatgag caagaggaca ttgaagaata tatgcaagca aatggagaaa taatggataa
     9061 aattcatagc gctttttttg agattgaaag ggaatacaca attgaaatac cagatttaga
     9121 gttgcgatta ttgagcgata ttgttcaagg ataggagtga attcgaatga caatgggagt
     9181 aatttttgat atggatgggg tactcataga tagcgagaca ttttattttg agagaagaat
     9241 gaacttcttt caagaactgg agatagatcc aggatcaaac cagattgatg attatgtggg
     9301 gaaaacagaa gctgcaatat gggaaacatt agttcctgag gacaaatcat taagagagga
     9361 actttatccg aaatatgtag attatcgaaa aacgcatcca attgatttcg ctaaagcttt
     9421 acgaaaagaa gtgaaaccgt tacttgactt cctcaagaag cgggatatac cattggcact
     9481 ggcatcttct tctccagctg tcgagataga gagtatgctc attcaatgtg aacttactga
     9541 atatttttcc tttgttataa gtggtgagtc attaaaacaa agcaaaccac atcccgaaat
     9601 atatttaaaa gcgaaacagc aattagactg tgacttcttt attgcagtag aggattcaac
     9661 gattggtatt aaatcagcaa aggcagcaaa tatctatacg gcggcactaa aacaaccaat
     9721 gcaaatcgat cagagtgaag cagacataca aattgaaagt ctacaagaac tggaacaaat
     9781 tgttttagaa aaaggttagt gaattaatgg aaataaggct atatatctgt gctagctatt
     9841 ttgactaacc aaaaaaactt gctataattc aggtgtagta aaaggtgctg gtaacacctt
     9901 ttacgaaaat gcttcggcag tgataatggg attggctttt taagtttttt attttgttcg
     9961 caatccctaa ggtcatgtga atgatcctag ggattgctag aatattttgc aacagaacca
    10021 aaatacgaat agttatcaag cagccatatt gactgcggtc aatttaggag gggatactga
    10081 tactactgct gccgtaactg gtgctcttgc gggtattctt tacgggggtg aagtgatccc
    10141 tgaaaattgg agaaaatcgc ttcaaaatga ccagcttctt aaaacgatca gtcagagatt
    10201 ttgcgatacc gtgctatctc aaagaatttg aataatggat atgatgcaaa tcttgtttgt
    10261 actaacgttg tattgacaaa aaggatttta tttagtacaa tgttagtaca aatgatgaac
    10321 ggaggtagaa tcatgtctca atcaaccatt acatttagga ttcccgacga tgaaaaagaa
    10381 ctggtattag aatatgcaaa agcacacaat tcttcattga ccgaacttta tcgaaatgct
    10441 gtactggata aaatcgaaga tgaaatcgat ctacggactt tacggagtgc aattgaagtt
    10501 tccaaagaga aaaaagagag tggtatttct caagaggaaa tggagaaact gctaaatgaa
    10561 ttataaagtc atctacatgc cgcaagccca gaagcaatta aaaaaaatgg acagaaacca
    10621 agctaaaata attgtcgcct ggatcagaaa gaatttagaa aacacgactg acccgcgtca
    10681 gcacggaaaa ggtttgactg gaaataagag tggggaatgg cgctatcgaa ttggaaacta
    10741 tcgaatattg gctaatattt gtgattctga aattgaaatc cagatattct caattggaca
    10801 tagaagtaca atatataaaa gataaccgtt tttggaacga agtagagttt cgaaagtcaa
    10861 tgtataatct tttgaatttt gcataatacc acaggtagaa actacccgat actatcggtt
    10921 ggtttctgct tttttatggc tctttgtcaa ataggactga tagagtgaga taagaacatc
    10981 taaggtagaa ttccttttct actttagatg ttttttgatt cataggtaat cagattattt
    11041 agtaagaaaa ttctggtctt cattttccga aagcttcgaa agccataagc gactcgtttc
    11101 aatgctttga tgtgggtatg cagattttct attttggcat ttgaataagg caattcgagg
    11161 gctaaacgaa tgccggattc atagtttata aaagcttttt ttatagaatg cagttctttt
    11221 ggaacggaat tgggcatcgt tcttacaagt ttaaagaaag tgtccgcctc tttgtcgtga
    11281 aacgcttgaa gaatttcttg atagtaaaaa taggcgattc gtaattcgtc agagatcgaa
    11341 agcagacgat ccaccacttc actttcggtg agatacgccc aaccaaaatt gcgccatttg
    11401 gtccatttgt cataaacaag cttgtcctgt cgtttgatca gaagccgaga caacgccttt
    11461 aattgtcgat aatatttggc ttgtgttgga tcgtttcgat caaagctttt catgattcgt
    11521 acgcgaatat cctcaaattt acgaatgaga tgtttgacaa tgtgaaaacg atccacaacg
    11581 agttttgcct ttggaaaaca ttcctttatc agagaggcat aagaaacatt catgtccgtc
    11641 acgatgacct ttaccttcaa tcgtgcgcgt cgttcaaatt gcataaaata agaaacgaga
    11701 gcctttttct tccgtgaagg aagaatatcc agtaattttc cagtatcgcc atccatacaa
    11761 gaaaaggagt agggaccgac ttgatttttc agtgaacgaa attcatccat taataaacaa
    11821 cgcggtaacc aatttctttt tacttttgtc atggggatca gagaagcgag tgtccgagaa
    11881 acggtcgacc aagaaacgcc gcaatgctta gcaattgtcg tcattgcttg tttttcagtt
    11941 agctcttcta aaattttata gcgcacttgt ttagcgataa agcagtttct atcgacaatg
    12001 taggtattcg ccgtaaaata ggtgtggcag ccacgacagt ggtagcgttg tttcgctaaa
    12061 gcaagataag tcagttggtt cgcacattta ttaagttgaa tgagcgattt tttctttccg
    12121 ttcttaacga tcatgccttc atgattcatc tgtttacaca aaggacagcg acgaatttct
    12181 ttacttaacg tcgcagaaac aacaagactc ttgatattct taatgctctt cgattccaca
    12241 tttgtgatga aaatgttttt atctttgatt ccaagtagta gtttgatatg atgaagatca
    12301 ggcatacaga acgctccttt gattgtgttg tggtgat
//
LOCUS       DAZKNH010000077        12197 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000077 DAZKNH010000000
VERSION     DAZKNH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.077"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(159..323)
                     /locus_tag="ACG8MG_002012"
     CDS             complement(159..323)
                     /locus_tag="ACG8MG_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376038.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            618..1946
                     /locus_tag="ACG8MG_002013"
     CDS             618..1946
                     /locus_tag="ACG8MG_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFQ0376039.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2148..2981
                     /locus_tag="ACG8MG_002014"
     CDS             2148..2981
                     /locus_tag="ACG8MG_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFQ0376040.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2997..3734
                     /locus_tag="ACG8MG_002015"
     CDS             2997..3734
                     /locus_tag="ACG8MG_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFQ0376041.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3889..4857)
                     /locus_tag="ACG8MG_002016"
     CDS             complement(3889..4857)
                     /locus_tag="ACG8MG_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFQ0376042.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5089..5922
                     /locus_tag="ACG8MG_002017"
     CDS             5089..5922
                     /locus_tag="ACG8MG_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFQ0376043.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5956..6795
                     /locus_tag="ACG8MG_002018"
     CDS             5956..6795
                     /locus_tag="ACG8MG_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0376044.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6821..7381
                     /locus_tag="ACG8MG_002019"
     CDS             6821..7381
                     /locus_tag="ACG8MG_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0376045.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7580..9301
                     /locus_tag="ACG8MG_002020"
     CDS             7580..9301
                     /locus_tag="ACG8MG_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFQ0376046.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9466..10608
                     /locus_tag="ACG8MG_002021"
     CDS             9466..10608
                     /locus_tag="ACG8MG_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0376047.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10624..11835
                     /gene="thiI"
                     /locus_tag="ACG8MG_002022"
     CDS             10624..11835
                     /gene="thiI"
                     /locus_tag="ACG8MG_002022"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFQ0376048.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4154 a         2048 c         2534 g         3461 t
ORIGIN      
        1 gttcgagtcc tactgccgga gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact
       61 tgggtttctt tttcttttat tattatgatg aatcaagctt tctgtttttg tcacgtatgt
      121 tgtaatcttt acgaaagagt agagttggct actcataatc aaccatactt cccatcatcg
      181 ctaaatacga aataaatcag cattcctaca ccaatcaaag cacaagctgc accaagaaaa
      241 gggatgacat tcccaaagtt atctgtattc ataagtatgc caccacccac aaacaaaaag
      301 ccgccagcaa tcaggttttt cattttattc atctcctttc ttacgttata gtatttcctt
      361 ctttcaatga gtattttgaa agctatctta ttgtatgatg ttcttctagg cctcttcttt
      421 attatacagg ccattttcgt tatctcatag aaatattcct ttttatctga aaactattgt
      481 ataccgtatg acatttttct tcatgaagtt gtaagtatat tctcattcaa atgaaaattt
      541 catcatagaa gagattgtat ttctaaattc ttaagagtaa aatacgaagt ataacttgga
      601 tttggaagga tgattggatg aaagctatta cgcctgatat gacaacaaaa tttaacaaac
      661 agtttcaagc aaatatcaaa cagcaggcat tgcagcgggc agttgtgaag aacggaatta
      721 ctgcttctgc tgaaaatgtt tcagcacatg tggagaatac accggtattt tctatagatt
      781 tggctacggg gaaagtggct aatcaaatgc aatcggggcg ttgctggatg tttgctgcac
      841 tgaacacatt ccgtcataag atgctgaatc aattccattt aaaagagttt gaattatcac
      901 aaaattacac ttttttctgg gataaatatg aaaaggcaaa ttatttctat gaaaacatca
      961 ttgcaacagg aaaagaggca atcgatagcc ggaaagtagc tttcttactc gctactcctc
     1021 aacaagatgg cggccaatgg gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc
     1081 ctaaatcggt gatgccggaa agtgcgaata gttcaaattc tcgagatttg aataactact
     1141 tgaacaaatt actgcgtaaa gatgcagtag tgttacggaa aatggttgct caaggagaaa
     1201 cattagacgc tattgaagaa aagaaagaag aaatgctaga aagcatttat aacttcttag
     1261 ctatctcatt aggaacacct ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga
     1321 actatcatct tgaccgagga ctgacaccac aaatttttta tgataaatac atcggtgtaa
     1381 aattagatga ttatgttagc gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt
     1441 cttatacagt agagatgcta ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg
     1501 ttgatatgga aactttcaaa aaattagcga ttgctcagct tgaagaagga gaatctgttt
     1561 ggtttggttg tgatgtaggt caatcttcta ctagaactag tggcatcatg gccttagatg
     1621 cttattcaat ggatgatcta tttgatacgg attttacaat gactaaagct gaacgtttag
     1681 attttggaga aagtctgatg acccatgcga tggtattgac cggtgttgac ttagtagacg
     1741 ggcaatcaac aaaatggaaa gtcgaaaata gctggggcga aaaagttgga aagaatggct
     1801 tctttgttat gagtgatgcc tggatggacg aatatacgta ccaaatcgtt gtaagaaaag
     1861 atttattgac gaaagagcaa ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt
     1921 gggatccaat gggtgcttta gcatagacga gtatttcaat gaagagtatg tagtaaagat
     1981 aaaaagagct ggcttaagcc agctcttttt atctttaaag tccattccct gttttttatg
     2041 gtccctgtgc tataattcta aataaaggat aaggattgga aataatcctt ttatcattaa
     2101 aaatatgatg acgaagtaga ataaaattgg gagtaggggg gaaagtcatg gatatccaaa
     2161 ctgttttatc tgatctgaaa ttaaatggta aagtgattcc agttgttggt ggcgatgtta
     2221 atcagaccta tcgtataaaa accgaacatc gtgcttattt tttaaaaatc catccgaatg
     2281 taaaaaaagg attttttgaa gcagaagtag acggattaaa agaactgtca gcattcgtac
     2341 gtgtaccgga tacgtatatg ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat
     2401 ggatcgagcc gggaaaaggg gatcaacgag atcttgctgc agctttagct aatctccatc
     2461 agcaaacagc gccacaattt ggttttcgta aagataacta tttgggtaca cttgttcaga
     2521 aaaattcttt tgaagaagat tggtggactt ttttctttaa agatcggctt gaatcccaga
     2581 tttctttggc agaagaaacg aatcgatgga atgtacagcg tcaagaaaag tatctgcgat
     2641 ttaaagaacg tgtattgaaa tcggttgaac caaaaaagat cactccacgc ctcttgcacg
     2701 gagacctgtg gagtggtaat gtgttctttg accagcaagg gcatccagtt tttgtcgacc
     2761 cagctgtttc ttatgggaat cgggaacaag atattgcaat gagccagtta ttcggtggtt
     2821 tccgaccgga atttttagat gcttatcaaa ctatatttcc attagaaaag ggatggaaag
     2881 accgactgcc gatttaccag ttatattatc ttttggcaca tttgaatatg tttggagaaa
     2941 gttacggttc ccaagtagat caattactag aaaattttta agaggagggc gtgtttttgg
     3001 caaaaaaata taccaaaaag catgaagttt catattatga gtgtgacatc aaccaaacga
     3061 tgacatttcc ttcgatgtta ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt
     3121 tgggaagagg cagtgaattc gtcaatagct ttggacttac ttgggtcatc acgaattatt
     3181 ccatggagat taatcgtctc cctagagtcg gtgaagtaat ctcagtaacg actcaagcca
     3241 tggagtacaa taagtttttc tgttatcgga acttctggat ccatgatgaa gatgggaaag
     3301 aattagtcaa aatagaatct gttttcgtat tgatggattt tgtgaatcga aaaatgagca
     3361 gtgttaatga agagatcatt gctccttttg agagtgagaa gatcaagaaa atcaagcgtc
     3421 aggaaaaaat tgaaaagatt gattcgggct tcatgttgcc atatcgtgtt cgtttttatg
     3481 atatcgatag taatcagcat gttaataatg cgatgtattt taattggatc attgacgtat
     3541 taggatatga ctttttgact acgcatgttc cagaaaaagt aattattcga tttgataaag
     3601 aagtagagta cgggaatgag atcgaaagcc attttgaaca aatccctatc gaaaatggtg
     3661 tcaaaacaaa acatgaaatc cgaatgcaag aacagctgta ttgtgaagca aatattatat
     3721 ggaaaaataa ataaaaaaga ggttgtgaca gaagggggca acttcaagaa ataagaagaa
     3781 atttacgaaa attgtttctc aaatttttgt aaattttcga cttagttttc gaagaagcta
     3841 tttttgtcac agcctctctt tttaagttta gctattagat aatgatgttt agttttggat
     3901 aaacgaaatc aattcttcac ccgacaaacc atagtttaca gcaatcaata gttttaaacg
     3961 tgctttttga ctattaatac tgttacagaa aataatcccc attttttcta gttcttttcc
     4021 cccgccttca taatcataaa caggctctgc gattccattg aaacaacgag aaacaagtac
     4081 tacagggatt ttttggacaa gaagcttttc caaagcagct aaggcttgtg gagggagatt
     4141 ccccgcacct aaagcttcga ttaccaaacc gtctactttc gtatgggcga tggcttccaa
     4201 taagtcccct tgcatcccag catatgcttt aacgatcggg atagtcccat ctacagcgga
     4261 aatgtccagt cgctttgttt ctaaaagttc ctgcaagaat aaaattcgat tcttagttac
     4321 taagccgatg ggacccaatg taggtgttcg gaaagtagca acatttgtgg tatgggtctt
     4381 tgtcacgtag cgtgcagaat ggatctcatc gttcatgacc accaaaaccc ccttatctag
     4441 cgcttcttca caacttgcga cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc
     4501 aagttcatta cttgaacgca ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt
     4561 tgtatcaaga aaaaaagcag tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc
     4621 ggatatacct gatgcataag cggacaagat acgttctttt aataccaaca tgtgagaagg
     4681 catgatgtgt ggtgagggaa gattgaagat atcttcaact aataattcta catgcttggg
     4741 gattgtgatt tctgcatcta ataatgggtt tgccacatca ggactaacac cgcccgtgag
     4801 catgttttct ttcatggcga tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt
     4861 acagacttcc ttacgaataa ttttgcatag ccgttaaatc ttattgtata ctatataaaa
     4921 gagttttgct atcagtgaag aataacgaat agcctaactt atcataaaaa agctgacctt
     4981 attaaaatgg aaaagactca accttgacct cctaatacaa tggagtatct ggttgagttt
     5041 cttttacaag aaagcagcca aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc
     5101 ggattaacat cgtttaaaga acatgacaaa ttaactggta aaaagaataa cactctttat
     5161 gaatatgcag ggtttctacc cttagtggaa ttagatactg cctattatgg aattccccga
     5221 aagtcctcag ttgagaattg gataagtgaa gtacctgaat catttcgctt tgtagtcaaa
     5281 gtctatcaag gaatcagcgg tcaaggagac tggaagcctt attatcagac cgaagaagag
     5341 atgattgcgc attttttaga tgttatgtcg ccaatggtag atgctggaaa gatgttttgc
     5401 tttttagtcc aattcccctc atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc
     5461 aaacttagaa agtggtttga gggttatcct gtttctatag aattgcggga ttattcttgg
     5521 tacagcgatg aatttcttga acgtacaaga aactttatgt ctagagccgc tttttcttta
     5581 gccatcatcg atgagccaca gttgctgaat acaacgattc cttttgatcc atttgtcacc
     5641 aatccgaact ttgctctttt tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat
     5701 gataaagagt ggcgtaaaaa aagaacgtta tatcgttatt cagaaaaaga actgcaagaa
     5761 ctagcacatg aagtcaaagt agttgattct aagacaaaag aggtaggtgt gatttttaat
     5821 aataattctg gtggtgatgc ggctgaaaat gcattagcac tgcaaaaaat attaaatatc
     5881 aagccagatg gactcaatcc aacccaaatg gatctgtttt aagggcttat tttattcaag
     5941 aaaggggcga agtctatgat caaagctatt ttttttgata ttgacggaac attggtcacc
     6001 aatcgttcaa aagcgttgga atcaacaaaa caagcaattg aatatgctcg gaaaaacggc
     6061 attttatgcg gtgtagcaac ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa
     6121 ttggaattag atatgtatgt cgtgtataat ggtcaactgg tttttactgc agatcgcacg
     6181 attatcgacc acccttttga acaaaaagta ttggaacata ttgttgaatt tgcagatgag
     6241 aaccatcgac aaatcgtttt tggtgcgaga aatcggttag atggatcaac gacgatgcta
     6301 ttaggacaat cgatttttat caagcgacta gtcagctttt taccgcgtaa attcccggta
     6361 cgtctgatga aaaaaatcct ccaggttttt agccctcacc gacaaaagga tcgttatgaa
     6421 acgttagaga tattaaaaga accaatttat caatgtatat tgctcagtcc ggagtcagaa
     6481 cagaaaaagt tagaacatcg ctttcccgag tgtacgtttc agcgttcaaa tacgtatact
     6541 gtagatatca tcccaaaagg tggttcaaaa ttactaggca tccaagcttt cgcaaatgct
     6601 gtcgaaattg aaatggaaga aattatggca ttcggcgatc attacaatga tatcgagatg
     6661 ctgaaaggtg taggaattgg agtggccatg gggaatgctc agatcgaagt aaaacatgct
     6721 gcagactttg tgactcaatc aaatgaagaa gatgggatat acgaaggact gaaacacttc
     6781 ggtctgattt attagtcaat gaaagaagga aaaaatcagt atggaagcag agacaccttt
     6841 tcaaaaagta gaaacattcc acaaaacttt tgatccaaga agaccagaga tacccacggc
     6901 ttttacagaa aatgaagctc atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt
     6961 tgttgcagct acttcaccta ataaagaaca actgcatgaa gctgttatgc tgctgcacga
     7021 agcattagat aaagcagaag aaaaaatact tggtaaaaca cagtggggaa gtgatccgct
     7081 ggttgaacaa gtagatgcac tgacagatat cctttacttt acttatggtt ctttttcatt
     7141 actaggaata gtgcctgatc gaatattcga aatcgtccat caggcaaata tggggaaatt
     7201 atttccagat ggaaaacctc attatgaccc tgtaacaaat aaagtgttga aacctgaaaa
     7261 ctggcagatg gaatatgctc cagaacctaa aatcgcggca gaaatccaac gtcagattca
     7321 agaagcgatt gctaagaaga aatccgctga tataggcatg tgcacaaatg aaaaagagtg
     7381 atcagagttt cactattttt gttatcttta tacgatggaa aaacaaaaga ttttcgttta
     7441 agttagtgaa tgcatgaaaa tagtatgcta taatctagaa tagatgtctt ttgcacgaga
     7501 gacgtattat ttggatttat gccaagcaga acgccatctg ttgcataaaa gatgcatact
     7561 gaaaacggag gaaccataaa tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat
     7621 tatagtcgcc attgtcgtct ttttggttag ttacttgatg aagaaaaaaa accaagatcg
     7681 attggatgcc ttagaaaaac gcaaggaaga attatttgac ttgccagtga ttgaagaagt
     7741 cgatgatgtt aaaaaaatgc atctggttgg gcaaagccaa aatacctttc gtgaatggag
     7801 tcaaaaatgg aatgacatat ctacttcctc gttcgctgaa ctagaaagtc aaattttcga
     7861 agtggaaaac ctcaatgaaa gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc
     7921 agaagctaca atggaccaaa tggagaaaca agtgacggaa atccgtgaag gtctaaagga
     7981 attgcgggaa acggaagaac gcaactcact agctgttcag caggcacttg acgtctatga
     8041 agaactgaaa gaaaaagtca gagaagacga gcatgaatac ggaccagctc tttttgaatt
     8101 gcaaaaacag atcaaagtga tcgaaaaaga atttacgcag tttgtgactt taaacacttc
     8161 tggagaccct gttgaagccc gcgaggtttt ggataaagca gaaaaacata cgtatgaagt
     8221 ggaagctctg atgaaaaaaa ttccaccgct gtatgaggag ctccatacca ctttccctga
     8281 acaacttgaa gaaatcagtg atacttacga gaaattgatg gaagaacaat atgtattccc
     8341 agaagaagat cttgctgaag atatcgcaaa agtaagtcga agaattgaaa attcattagc
     8401 caatcttgaa aaaacagaag ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt
     8461 gattgattcg ttatatgata tcttagaacg ggaaatggaa gctcagagat atgtaaaaac
     8521 taaccaatca acgattgcag agtatatcaa acatacaaca aagaataacc gtcaattatt
     8581 gattgagctg gatcacacat ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt
     8641 acgaggtttc caaaccgaag tggaagaaat ggaacgtcaa aatgaacaga tgattccaca
     8701 gatccgtcaa cacgaaattc cttattcaga agtgcgcacg ttctataaaa cagtcttcaa
     8761 agtcttagaa gatatcgaaa agcaacaagt agagatcgac gactcattac atgaattgag
     8821 aaaaggcgaa aaagaagcgc aagaaaagat cgatacattc gaattcaaat tgcgcagttt
     8881 gaaacgtttt gtagaaaaac aacgtttgcc tggcttgccg aatgattacc tagaattctt
     8941 ttttgttgct actgaccgta ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa
     9001 catggaagag gtcaatcgtt tagttgctct atgtgaagaa gatctagaat tattagataa
     9061 gaagacacat gatttagtag atgcagcagc attaacggag caaatgctcc aatatgcaaa
     9121 tcgttatcgt catacacacg aagaagtgag agaagcaatt gataaaagct actatttatt
     9181 caataaagaa tatcactatc aagaagcatt agacgaaatc ggtacagcat tagagcgagt
     9241 agaacctggt gctttcaaac gcatcgaaga tttttatttt aatcatccag acttagtata
     9301 aagcaaaaaa gttagtaaac cagcaagagc aagacggatt attgtcttgt tcttgtcttt
     9361 ttttgtatat ttgaaaattg aaacaagtga aaatttcacc tgctcttgaa agcaccttgg
     9421 acaaccgtta taatagaaaa gaatttcaaa aaagaggggt ttcttatgat atattttgat
     9481 aacagtgcaa caaccccaat ctttccgcaa agcttagatg catatgtaaa aaccagtcag
     9541 cggattattg ggaatccatc aagcttgcat gatttaggca atcaagctaa ccggttgttg
     9601 cagcaagcaa gaaagcagat cgctgatttg atccatgtag aaccagaaga aatctatttt
     9661 acttcaggcg gaacagaagg agataactgg gtattaaaag gaacgatgat cgaaaaaaga
     9721 gaatatggga atcatatgat catttcagga gtcgaacatc cggccgtcag cgagacagca
     9781 gaacaattaa aagaattagg aatcgaagta agtatcgccc cagtggataa acgcggattt
     9841 gtacgtgtcg acgaattgaa agaattaata agaaaagaga cagtattagt ctctgttatg
     9901 gctgtgaata atgaagtagg atcgattcag cccatccagg aaatcagtga tcttttggat
     9961 gattttccta aaattcattt ccatgtagat gctgttcagg cgattggaaa agtaccattc
    10021 tcgcaatggt tgacagaaag agttgatttt gcaacatttt cagctcataa attccatggc
    10081 ccacggggaa ctggtttcat ttattggaaa aagggtcgtc gtttagcacc attactgaca
    10141 ggtggagggc aagaaagaaa tcagcgaagt gggacagaaa atgttccagg tattgtggct
    10201 atggttaaag ctttacgttt atcattagag ataaaggaac aaaaacctga gcagacacaa
    10261 cgcttaaaag tagctctaat caatgcgttg aagacgtatg agaaagtgac gattttttca
    10321 gaaggaactg actatgcacc gcatatttta tgttttgctc taaaaggaat tcgcggcgaa
    10381 gttctcgtcc atgccttaga agaaaaacaa atatttgttt ctactactag tgcctgctcc
    10441 tcacgtaaac atatggcaag cagtacattg cacgcaatgc atgtaccaaa tgatctggct
    10501 acatctgctg ttcgaatcag cttagatgaa aacaattcgc ttgcagaagt cgagcaattt
    10561 atgattattt tcaagcagct ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt
    10621 cttgtgaaat acagtgaaat tatggtccgt tatggggaac tttcgacaaa aggaaagaat
    10681 cgaaaaagct ttatcatgca actagcacaa aatgtcagac aagctttgaa agactttcca
    10741 gaaatcaaaa tccatgctga tcgggaccgg atgcatcttt tattgaatgg cgcagacggg
    10801 cagctagtta ttccaaaact agcaaaaata tttgggatcc aaaatttctc tccaagtatt
    10861 cgtgtagaaa aagatgtcga agttttgaaa caagctgttc aagacatcat gaaagaaatt
    10921 tatactggga aagaaacatt taaaattatc gctaaaagaa gcgatcacca atttgaattg
    10981 acaagtaatg aattgaatca aacattaggt aatgctgttt ttgatatttt cccgcatatc
    11041 caagttcaaa tgcgtcaacc agatattcca ttacgtgtag agattcggcg tgatggcgct
    11101 tatctatcgt atgaaacgat caagggagct ggaggattgc ccgtaggaac aagcggaaaa
    11161 ggaatgctta tgctttctgg ggggattgat tctcctgtag cagggtactt tgccatgaag
    11221 cggggagtgg aaattgaagc tgttcatttt gctagtccgc cgtatacaag tgaacaagca
    11281 ctgcaaaaag caaaagatct aacagccaaa ctagcacctt atgtgggaac gatccatttt
    11341 atcgaagtgc catttaccga gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat
    11401 tggatgacaa ttactcgtcg aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt
    11461 catggtttag tcattttgaa tggtgaatca ttaggtcaag tggcttcaca aactttgcaa
    11521 agtatggtgg ctatcaatga agtaacgaat acgccaatca ttcggcctgt agcgacaatg
    11581 gataaacttg aaatcattga agtagcagaa cagatcgata catttaatct ttctatccag
    11641 ccttttgaag attgctgtac gatttttgct cctccacagc caaaaacacg ccctcaactg
    11701 gaaaaggtcc atcaatatga agagcgttta gcaatagatg aaatgatcca acgagcacta
    11761 gctggcttga agatggaaga gatacaagca gaatcgtcta atgaaaaaac tgaagaattt
    11821 tcagaattgc tataagaggt tatgaaaata gtgttttgac cagagtatta gagaaacaaa
    11881 acgaaaaaat agctccatgt attttaccgt ttttgaacta atgtcgaaga ttagctgatt
    11941 gaataccatt tatcaagaag ttatggaaaa gccggttctt ttttactaac tgaaaaataa
    12001 aattttattt agagatcaag ctacagttga taaatgatat aaaaaagaga atttcccatg
    12061 aaatagcgag gttgtgacag aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa
    12121 ttgcttttca aatttttgtg aaatttcggc ttatttttcg aagaagttac ttctggaaca
    12181 ccgtgtatca gaggttg
//
LOCUS       DAZKNH010000078        12150 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000078 DAZKNH010000000
VERSION     DAZKNH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.078"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..611)
                     /gene="frr"
                     /locus_tag="ACG8MG_002023"
     CDS             complement(54..611)
                     /gene="frr"
                     /locus_tag="ACG8MG_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFQ0376049.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(614..1336)
                     /gene="pyrH"
                     /locus_tag="ACG8MG_002024"
     CDS             complement(614..1336)
                     /gene="pyrH"
                     /locus_tag="ACG8MG_002024"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFQ0376050.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1472..2353)
                     /gene="tsf"
                     /locus_tag="ACG8MG_002025"
     CDS             complement(1472..2353)
                     /gene="tsf"
                     /locus_tag="ACG8MG_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFQ0376051.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2452..3234)
                     /gene="rpsB"
                     /locus_tag="ACG8MG_002026"
     CDS             complement(2452..3234)
                     /gene="rpsB"
                     /locus_tag="ACG8MG_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFQ0376052.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3593..4072
                     /locus_tag="ACG8MG_002027"
     CDS             3593..4072
                     /locus_tag="ACG8MG_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0376053.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4289..5380
                     /gene="phnW"
                     /locus_tag="ACG8MG_002028"
     CDS             4289..5380
                     /gene="phnW"
                     /locus_tag="ACG8MG_002028"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFQ0376054.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5373..5501
                     /locus_tag="ACG8MG_002029"
     CDS             5373..5501
                     /locus_tag="ACG8MG_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFQ0376055.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5505..6050
                     /locus_tag="ACG8MG_002030"
     CDS             5505..6050
                     /locus_tag="ACG8MG_002030"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFQ0376056.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6504..8195)
                     /gene="argS"
                     /locus_tag="ACG8MG_002031"
     CDS             complement(6504..8195)
                     /gene="argS"
                     /locus_tag="ACG8MG_002031"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFQ0376057.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8615..9562)
                     /gene="arcC"
                     /locus_tag="ACG8MG_002032"
     CDS             complement(8615..9562)
                     /gene="arcC"
                     /locus_tag="ACG8MG_002032"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFQ0376058.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9677..10696)
                     /gene="argF"
                     /locus_tag="ACG8MG_002033"
     CDS             complement(9677..10696)
                     /gene="argF"
                     /locus_tag="ACG8MG_002033"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFQ0376059.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10787..12016)
                     /gene="arcA"
                     /locus_tag="ACG8MG_002034"
     CDS             complement(10787..12016)
                     /gene="arcA"
                     /locus_tag="ACG8MG_002034"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFQ0376060.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3322 a         2526 c         2200 g         4102 t
ORIGIN      
        1 taaataaaaa cagaggaagc ggaatgatcc gcccctctgt ttttatgatt ttattaaact
       61 tccaataatt ctttttcttt atcagcagcg atcttatcta cttctttgat gctgttatct
      121 gtcaatgttt gtacgtcttt ttctaatccg cgcaaatcat cttcggtaat atcaccattt
      181 ttttgttgtt ttttatattc atcgatcgca tcacgacgga tgttacgaat cgcgattttt
      241 gcattttctg cttctttttt aacatcttta gctaattctt ttctgcgttc ttctgtcagt
      301 tgcgggataa ctaagcgaat gacatttcca tcattggttg gactgattcc gatatcactt
      361 gccataatag ctttttcaac atcttgaaga atgctcttgt caaaaggtgt gatcattaaa
      421 acacgtgctt ctggaatttg gattgatgcc atttggttca atggtgttgg cgcaccgtag
      481 tagttcacag taatacggtc caaaaggcta gcgttcgcac ggcctgcgcg gatttgccca
      541 agttcacgtt gaagattttg tgctgctttt ttcatttttt cttttgcttc tgtcataatt
      601 gcatctgcca tttttatttc ccccttacag ttgttccgat attttcgcct aagattgcac
      661 ggcgaatatt tccgtgttca tttaaattga agacaagcag tggaatatca ttatccatgc
      721 ttagtgaact tgcagttgaa tccatgactt gtaatccttt ggcaataact tccagatgag
      781 tcagttcttc aaattttact gcatttgcat ctactttcgg atcagcagaa taaacaccgt
      841 ctacattgtt tttagccatc aggataacat ctgcgccgat ttctgccgcg cgtaatgctg
      901 ctgttgtgtc tgtcgagaag tacgggttgc cggtaccgcc tgcaaagata acgatccgtc
      961 ctttttctaa atgacgttct gctttacggc gaatatatgg ctctgcgatt tgacgcattt
     1021 caatcgaagt ttgcacgcgt gtcggtacac ccacgttttc caacgtatcc tgtaaagcta
     1081 aggcattcat aactgtagct aacataccca tgtaatcagc ttgagcacgt tccatgccca
     1141 tttgtgctcc gatttgtccg cgccagatat ttccgccgcc cacaacgatt gccatttcaa
     1201 cgcccagctc gtgaacttct ttgatttctt ggatgatttc tttgattact ggtggtttga
     1261 ttccaaatcc tgcgtcccca gctaaagctt ctccactcaa ttttaagact acacgctgat
     1321 atttcggtgt gaccatgttc attcctccaa tattttctat tgccatttta ccatacttag
     1381 atgatttcga cactctaaga ctggtttttc ttgttttgct taaaaaaagg gagcgcacaa
     1441 accgtgcgtt cccgtctgtt attcaacaga attatttttt cacttggctc ataacttctt
     1501 ctacaaagtt atcttcgcgt ttttcgatac cttcgccaac ttcgaaacgt acgaatgttt
     1561 taacagttgc gccttttgat gcaacgtatt tttcaacagt catatcagga tctttaacga
     1621 atggttggtc caccaaagcg atttctgctt tgaatttctt caagcgtcct tctaccattt
     1681 tttcaacgat attcgctggt ttgccttcgt tcaatgcttg ttctgtaaga acagtttttt
     1741 catgttctaa ttcagcttct ggaatttgtg attcgtttac ataacgagga ttgatagctg
     1801 ctacgtgcat agctacgtct cttgcaactg tttcgtcagt tgttccatct agaacagtca
     1861 atacggcaat acgtccgccc atatgcaagt atccaccaaa tgcagcattg tcgtcttttt
     1921 ctacaacttc aaagcgacgg aagctgattt tttctccgat aacttgagtt gcttcgatca
     1981 agtcagattc aattgtacct ttatctgttt tgattttcat tgcagcttcc atgtctgctg
     2041 gtttgttttc agcaacaagt tctgcaattt cttttactaa atcttggaac atttcgtttt
     2101 tagaaacaaa gtctgtttct gagtttactt caacgattgc tgcagtgtta cctttcacag
     2161 caactgaagc caatccttca gctgcaatgc ggtcattttt cttcgcagcc tttgccattc
     2221 ctttttcacg taaaagatca actgcttttt ccatgtcgcc ttctacttca actaatgctt
     2281 ttttcgcatc catcattcct acgccagtca tttcgcgtag ttcttttacc attttagctg
     2341 taacgtctgc catgtttaac tcctcctgtg tgtttttctt tttaaaaaaa gctgcttcaa
     2401 aggagagtga ggcttgtcgc ccagcctttg aaacagctcc atcattcaaa attattctgc
     2461 tgaagaattg tcgccttcaa caacatctac gatttcttcg attgatgttg ctgtgttttc
     2521 ttctgtgaaa tcttcttcaa ctacttgatc ttcaccttgg tttccttcga tgaaagcatc
     2581 agccattttt gaagtaatca atttaacggc acgaattgca tcgtcgtttg aagggattac
     2641 tacgtcgatt tcatctggat cgcagtttgt atcaaccata gctacgattg ggatatttaa
     2701 tttgtgtgct tcttgaacag caatacgttc tttacgtggg tcaacgatgt acattacatc
     2761 tgggattctt ggcatatcag cgataccgcc taagaatttt tcaagacgtt cacgttcttt
     2821 gttcaaacca gcaacttctt ttttaggtag aacttcaaaa gttccatctt cttccattgc
     2881 attgatttgt ttcaaacggc tgatacgttt ttggatagta tcccagtttg tcaatgttcc
     2941 gcctaaccaa cgatggttaa cgaagtattg tcctgcacga gttgcttctt ctttgattgc
     3001 ttcttgtgct tgtttttttg tacctacgaa taatgctacg ccgccttctt cagcaacgct
     3061 cttcatgtaa tcataagctg catctactaa tttaactgtt ttttgcaagt caatgatgta
     3121 gattccgttt ctttctgtga agatatattt cttcattttt ggattccagc gacgagtttg
     3181 gtgaccaaag tgtacgccgg cttctagtaa ttgtttcata gaaattactg ccatgtttgt
     3241 ttcctccgat ttggttttat ttttcctctt cttatcttct accttgccgg caaacttttt
     3301 caagcaccta ccgacaatcc aataagaatg tggatttgct gatgcataaa gcaccctcag
     3361 ctattataca gacaaaagat ccgtatcgca agagcaaata tacttttctg ctcaacattc
     3421 aatagtttac ctgaaagata aaaagaattc aagaaaaaca gtagatttct ctttgtttct
     3481 ttattatcct ttttccataa aataatttta ggtataaaag acgaatatta ttgggcaatc
     3541 cttctttttt ttgttatgat acatgtgaaa tctttcgtaa aggagaaaga aaatgcgcaa
     3601 agctcaaaga catttgctga ttaaacaagt cattgaagaa ttcccgatcc gtacgcaaga
     3661 agaactatta aacaaacttg ggcattatgg cgttaccgcc acgcaggcaa cgatttctcg
     3721 agatatccgt gatctaaaaa tcgtgaaggc acctgatgaa aatggcgttt cccgcttcgt
     3781 attgtttcgc gaaaaaggaa ctcctgagac taggactgag gaagaggatc ggctggttca
     3841 aatgattgaa gacattgtat tgaaagtcga acgagtccac tttttgacga tcgtcaatac
     3901 cttgccagac aatgctcatt tatttgcatc tgttttagat gagatcaaac ctccctctat
     3961 tgtcagtaca attgctggat tcgatacgat catggtcatc tcggaaaatg aggaggacgc
     4021 aaaacgagta gaagaatttc ttcaccatcc aactgaagta tcactttttt aatcagagca
     4081 cgtattccta atatggaatg cgtgtttttt tattttcaat attttcctta ttgaataaac
     4141 ctttacactc tttacaaaaa tacatcattt tttattcaaa tttttcaaca ccttgattga
     4201 atcacaataa tcatcattta tattggatgt taacaagagc agcttcttgc tctttgtgta
     4261 tttcaaaaaa gaaatgaaag gaaatcatat gtataaatta ttaacttctg gaccattgac
     4321 gacttctgaa acagtgaaaa atgctatgct gatcgatcat tgtacttggg atcaagaata
     4381 caaagaaatt acacaatgga taagaaaaaa attattggat cttgctcaag tatcggaagg
     4441 aatatacaca acggtcttaa tgcaaggtag cagctccttc ggggtagaat cggtattaag
     4501 ttctgtgatc aggccagaag atactgtact gatttgttca aacggcgcat atagacaacg
     4561 aatgatcaac atgtgcgaat gtctgaaact gaactatacc gtttatgagg tatcggaaca
     4621 tcaaatcctc caagctcatg tgattacaca gctgattgaa gagaatccgt ctatctccgc
     4681 actagcaatg gtccatagcg agactacttc cggtattttg aataatttgg aagagattgc
     4741 tgctgtcaca caagaaagag atcttttgtt tatagtggac gcgatgtcaa gctttggtgg
     4801 gataccgatt tgtgtagccg aactagggat tgattttttg atttccagct caaacaaatg
     4861 tattcaaggt gttccaggct tttcttttgt acttgctaat aaagcggaac tggaaaaacg
     4921 aaaaaatgtt agccgaagtt tgtcacttga tctatatgac cagtaccgcg tgatggaaaa
     4981 cgagaatggc aaatggcgtt tcacttcccc tacccatacg gttttggctt ttcaacaagc
     5041 gctgaaggaa ctagaggcag aaggaggaat cgaaactcga tattcacgtt atctcgccaa
     5101 taatcatgtg ctacgacaaa aaatgacaga gatggggttc cagcctttca ttcaagaata
     5161 tcaggggcca ttcatcacct cttttcttta ccctaaggac tggcgatttg attttaatca
     5221 tttttatcag tatttaaagc aaaatggatt tgtgatctat cccgggaaaa tcagcgatca
     5281 tgaatgtttt cgtatcggaa atattggcga gatctatccg gaagatattt tggaactgac
     5341 agcaattatc aaagactata aggagaacgt taatgattaa aactgtaatt tttgattggg
     5401 caggaacaac tgtggacttt ggctgtatgg ctcctgtcca cgcttttaga aatgcttttc
     5461 tagaaaaagg aatccaactg actgacaaag aaatccgcta accgatggga aaattaaaat
     5521 gggatcatat ccaacagctt ctttccctcc cttctgtcaa aaaacaatgg atagctattt
     5581 atggacaact acctcaggaa aaagaggtgg aggaattgta tcaacaattt gaacattatc
     5641 tgtttaaaga tttggtcaag cattctacct tgaaacccga cacattggaa acgatcgaac
     5701 ggttgagaaa cgctgggatc aatattggtt ccacgaatgg atttaatgct cctatgatga
     5761 caatcgtagc agaaaccgca aaaaaagaaa ggtattcacc agacttcatg gcaacttttg
     5821 aagacgtcga agggtatggt cgaccgtttc cttatatgat ccataaaaac atgcagtact
     5881 tcaaaaacaa gtcggttgat gaagtaatca aagtcggaga tacggtctca gatatccagg
     5941 aagggaaaaa tgccggagtg ttgacagtag gtgtaattga aggtagttcg ctaatgggga
     6001 tttcttatga tgagtaccaa aaattagaga tggggaaaaa gatcgtttaa aacagtctgt
     6061 tgctcttaga ttccaacaag ccggcgctga tatttgtgta aacaatttaa aagagttaac
     6121 agaatacatt ttgtacgctg acatgaatcc agaatgactt ttatacaaaa taagaggttg
     6181 tgaaaaagct gtcctgtatc aagtaataaa aacagccaag caaaaatagc tcctcatatt
     6241 tttcgtttgg ttaggcttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
     6301 aaaaaaggga gctgtgacac gacttttgtc acagtcccct tatttgcaaa caaacagtgt
     6361 ttaagagaca acctctccac taaacggtgt tcattcagct gactctcggt atttgctcaa
     6421 aaaacgataa aatttgaaaa gctattttct cttttgtttc tctaatactc ggatcagaac
     6481 gctgatttca cattctcttt ttcttacatc ttatctggtg catgaagtcc tagcaaacga
     6541 aggtcttctt tcaataatac agtcactgca taaactagag ctaagcgtgc ttctttttgt
     6601 tcatcatcag ctaaaatctt cgtatgagca tagtatttgt tgaatgcttg tgctaatttg
     6661 attgcatgtt tcgcaatcac tgacggttcg tatttttccc cagcagacaa gaccgtttca
     6721 gggtattttt gaactaattt aaccacttcc cagctggtat cgtcattcaa tgcatagtct
     6781 gctgtttctg aagggacaaa tcctgctttt tccaacaggc tgactgcacg agcatgtgta
     6841 tattgcacat aaggtccagt ttctccttca aatcgaacaa cttcttccag gttaaagtca
     6901 aatgtattca gtcgatcgtt tttcaaatcg tggaaaatga ctgccccaac cccaacttgt
     6961 ttagctacag catctttgtt ttccaaatca gggtttttct cgctgatttg ttctttagca
     7021 gattggattg cttcatttaa tacttcttct aataagacga tctttccttt acgagttgat
     7081 aattttttac caccttgcgt gatcaaacca aatggaatat ggtgcatgtc gtcagaccag
     7141 tcaaagccca tttccttcaa gacagctttc aactgtttaa aatgatagct ttgttcattc
     7201 cctactacat atagagattg tttgaagtcg taagtacgtt tacggtacaa tgcagcagct
     7261 aagtcacgtg taatgtatag tgtcgcaccg tctgacttct taatcaacgc tggattcagg
     7321 tcataagcag ataggtcaac gatctctgca cctttatcct cgttcagcaa atgtttttct
     7381 tctaaaagtt tgacgatttc atccatcttg tcattgtaga aagcttcacc gttcaacgaa
     7441 tcgaaacgta cttccagcag atcatagatt ttattgaatt ctttcatcga ttcatcacgg
     7501 aaccattgcc ataattgaat agcctcttta tcgccttctt ccagacgttt gaaccaagca
     7561 cgagcttctt catttaattc tggttctgtt tcagctactt catggaattg gacgtataaa
     7621 cgaagcaatt cattgattgg ttctgcttta acagcttctt ctgtacccca ttttttgtaa
     7681 gcgacgatca gttttccaaa ctgtgttccc cagtcaccta aatggttgat gcggattggc
     7741 tgataaccga ttttttccat aatgaaaccg atcgaattac cgataactgt cgaacgcaaa
     7801 tgtcccattg agattggttt ggcaatatta ggagaagaca tgtcgattgg tactgttcct
     7861 tgattcccga tgttgctatc cccataatgt tctttttctt tcactactgt ttgcaaaact
     7921 tttttgctga taagttcttt attcatgaaa aagttcaagt aaggtccgac aacttcgatt
     7981 ttttcaaagt tggcagaatc gattttttct gctaaatctg cagcgatttg ctgtggcgct
     8041 tttcggtaaa cttttgctaa tgaaaaggct ggaaatgcaa catctccgtg ttcagctgat
     8101 tttggatttt ctagtaattg ctcgacttgt tccagtgtta aatcatcttt gacagcgtca
     8161 tagacagctt ttgcgacaag atctttatta ttcatcttta ttcctcctaa agtgttccct
     8221 tgattgttaa acaaaaaaaa tctctagcga cggcatactt agtatgccat cactagagac
     8281 gagatttccc gcggtaccac tccaattgtc ctaaaaaagg accagctcca tctgatatcg
     8341 gtagacgacc gtctgtttaa aacagaattc tccaagtgcg cttcgttgta tcttcttcat
     8401 tcggcttcca ccacttccga attcgctagc caagaatgat acaagtactc tcttgttcaa
     8461 caaatcttag tcttattaaa ctttcttgaa ttatatcaga aaaaaaaacg aaaggctagt
     8521 cgtttcataa aaaaacaatc cagatggcct aaaggagaag aaataccacc tggatagcta
     8581 taaatcattt cgtaaatacg aaagacttaa caaattaatc agctacgata atcgttcctt
     8641 cttcagaagc taaaagattc tcgatatttt ctaaagaagt gatgattgcc ttagcgtttg
     8701 gttttgcttc aacaaatgca atagctgctt ctactttcgg aagcatgctt cctggagcga
     8761 attgcttttc atcaatgtat tgtttcattt ctgaaacagt aacagtttct aatttctttt
     8821 gatctggttt ttggtaattg acatacacat tgtctactcc tgtcaatacg atcagcaaat
     8881 ccgcatcgat gatttcggct aatttttctg aagcaaaatc tttatcgatt acggcttctc
     8941 gtccttctaa tccagtagct gtttctacaa caggaatccc accgccacca acagagactg
     9001 tgataactcc ttgatcgacc agcgtttcga tgacacgtgc ttctttaatc gagatgggtt
     9061 taggggaagc gactactttt ctccatccac gtccagcatc ttctttgaat gtcacagtgg
     9121 agtcagcatt catttgttct ttggcttctt cttccgtgta gaatggaccg actggtttac
     9181 ttggattttt gaatgaagga tcattttcat ccacaataac ttgagtgact aaagaaacca
     9241 catctttatc gatccctttt tccttcagta cttcgcccat ggcgttttgt aaccagtaac
     9301 cgatagatcc ttctgtcatt gcaacacatg tatctaaagg cattgccggt gttttctcag
     9361 aatcagcagc ttgctgctga atcaataggt taccgacttg cggaccattt ccgtgggaga
     9421 tgatcaattc atctccttgt tcgataaatt tcactaaata ctttgctgtt tccattaacg
     9481 cttcttgttg ggcttttgca cttgcatccg tagacaagat ggcattgccc cctaaagcga
     9541 caactacttt tcgtttggcc atttcattcg tcctttcttc agttctaccg aattttctag
     9601 ataacaaaac gagccagcgt tttgtgattt tccctcaatg ctgactcgtt ttttcgtgag
     9661 atgaatgtgg ttatgcttaa acgcgaggga taaacaagtt acctaatgtt gctgccatga
     9721 tcgctttgat tgagtgcata cgattttctg cttggtcaaa ttgacgagca tatttgctgc
     9781 ggaagacttc atctgttact tccatttcag tgatgccaaa gcgttctttc atttgttcac
     9841 cgtaaacagt gtttgtatca tggaaagctg gcaagcagtg caagaagatc aaacgatctg
     9901 tgttgtgtgt tttttctacc atttccatat tgatttggta aggtttcaac aatttgatac
     9961 gttcttcgaa tttatcttct tcacccattg atacccatac atcagaataa aggacgtctg
    10021 ctccgtctac acctttatca acatcgtcgg taatcatcaa ttgtgcaccg gatttttcag
    10081 caaagccttc tgccattttg acgatttctt cttcaggttg taattctttt ggtgcaacga
    10141 tacgcatatt cgctcctaaa attgcacctg tgactaataa agagttagcc atattgtttc
    10201 gaccatctcc gcagtaagca acagtgatac cttctactgt tccaaagttt tcttggatag
    10261 ttaaaaagtc agcgatcatt tgtgttggat gccattcatc agtcaagcca ttccaaactg
    10321 gaacaccaga gaattctgct agttcctcta ccattttttg actgaatcca cggaattcga
    10381 ttccgtcaaa catacgtcct agtactttag ctgtatcttc tgtggattct tttttaccta
    10441 attggatatc atttgcacct agatattcag gatgtgcacc tagatcgatc gctgcagtag
    10501 taaatgcaga acgagtacgt gtagatgttt tttcaaataa aagcgcaata ttttttcctt
    10561 ctagatagtg atgtgggata ccacgttttt tcaagtcttt caagtgttct gaaaaatcga
    10621 tcaaatattg taattcttct tttgtaaagt ctttttctgc taataagctt cttccttgga
    10681 atactgattc tttcatatta aacattctcc tttagattgt ttttatttaa gattaaagag
    10741 gaaatcccga cggcagctga cagtaagact gccgtcagga aacaaattac ttcaagtctt
    10801 cacggacaag tggttggctc atgcagcgtg gaccaccacg acctcgtgac aattcacttg
    10861 aattgatttc taacactttg atgccatagc tacgaagcaa ttcgttcgat acataatttc
    10921 ggttataagt gactacaaca cctggagcga ttgccaatgt gtttgatcca tcattccatt
    10981 gttctcgagg tgcaacgatc tcatctccat taccagttgg gatcagtaca agatcatcta
    11041 atccaagagc agctttcatc gtttcgtgta agttatctga atgagtaatc ttgatatcat
    11101 caccatctgg ttcgattgta aagacatcca tcttaccgtc tttactttga attgctggat
    11161 ggatagtgaa tttatcgtga tctaccattg taaatacggt atccaaatgc atcattgcac
    11221 ggttgttcgg aattttgatt gccaacactt tttcaaatcc tgaatttttt gcgaataggt
    11281 ggcgtgctaa tttttctaaa gctttcgcat tagttcgttg agaaatccca acagctacta
    11341 ctttatcgct taagatcaat tcgtctccgc cttcgatatg atctggattt tcacgatcca
    11401 accatacttc gattccttta tttgcaaatc ttggatgatg tttcaaaata tattctgtaa
    11461 acatcgattc acgacgacgt gcttcaaaag tcatcttatt gactgtcatc ccatttccca
    11521 ttgatgcgga agggtcgcgt gtaaagtaca agtttggcat tgggtccatg taaaatggat
    11581 agtcatcgtc tgctgacaga tcatatagac tgctagaacg tacttcgata tcttttgtac
    11641 gaaccccagc catgatttta tctaccattt cttgcgtttc cattgataga aggaactcat
    11701 gaagaccctc tcttacagca ttcgatgcaa tatgagattc atccaacatt ttattcaaga
    11761 attgttcttt gacattgcct gcgtcaatcg cttctgccgc taatttttcc aagtaaagag
    11821 tctcgacgcc ttcattttgc aatgtcttcg caaagttgtc atgttcttct tgagcaatcg
    11881 gaagatatgg aatatcatcg aacaacagac gatccataat atctggtgtc aaattttcca
    11941 cttcttgtcc aggccgtttc agtaaaactg tctttaattt ccctatttca gagaaaacgt
    12001 gaataggttt atccatgttg tgttgcctcc ttctttttga ttacatgtat aggatatcct
    12061 ccttttgtaa tcactttcat aattttgtct cattcaaaat gagacattcc tcacattaat
    12121 attcgataat taaataatta aacgtttttc
//
LOCUS       DAZKNH010000079        12025 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000079 DAZKNH010000000
VERSION     DAZKNH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.079"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(126..908)
                     /locus_tag="ACG8MG_002035"
     CDS             complement(126..908)
                     /locus_tag="ACG8MG_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376061.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(973..1809)
                     /locus_tag="ACG8MG_002036"
     CDS             complement(973..1809)
                     /locus_tag="ACG8MG_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0376062.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1827..3236)
                     /locus_tag="ACG8MG_002037"
     CDS             complement(1827..3236)
                     /locus_tag="ACG8MG_002037"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0376063.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3250..5073)
                     /locus_tag="ACG8MG_002038"
     CDS             complement(3250..5073)
                     /locus_tag="ACG8MG_002038"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0376064.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5916..6242)
                     /locus_tag="ACG8MG_002039"
     CDS             complement(5916..6242)
                     /locus_tag="ACG8MG_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376065.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6288..6650)
                     /locus_tag="ACG8MG_002040"
     CDS             complement(6288..6650)
                     /locus_tag="ACG8MG_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFQ0376066.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6687..6901)
                     /locus_tag="ACG8MG_002041"
                     /pseudo
     CDS             complement(6687..6901)
                     /locus_tag="ACG8MG_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7029..7364
                     /locus_tag="ACG8MG_002042"
     CDS             7029..7364
                     /locus_tag="ACG8MG_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0376067.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7440..9437)
                     /locus_tag="ACG8MG_002043"
     CDS             complement(7440..9437)
                     /locus_tag="ACG8MG_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376068.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9535..9810)
                     /locus_tag="ACG8MG_002044"
     CDS             complement(9535..9810)
                     /locus_tag="ACG8MG_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0376069.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10042..10776
                     /locus_tag="ACG8MG_002045"
     CDS             10042..10776
                     /locus_tag="ACG8MG_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0376070.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            10815..11957
                     /locus_tag="ACG8MG_002046"
     CDS             10815..11957
                     /locus_tag="ACG8MG_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0376071.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         3892 a         2398 c         1842 g         3893 t
ORIGIN      
        1 atatagtcgg gctccaaatc cgaaaaaaaa tacccccagt agcttatcct aagctactgg
       61 agatgtataa gtacggtcat ataacaacgg ggtagggtaa ataaatgaaa aagatggaag
      121 aatcattatt caataacttt tttaaatact tcgttgtaat cagttacctg tccgttctta
      181 aataccacag aataatcttt tttcccattt tcaagtaacc gaagtggaat gtctttacca
      241 acttgctctg tcactgcatt actgatacct gttagattag ctaataaatc tgataaagat
      301 tgagcatctg tagaaatctc tgtatcagta tttatcgtga ttcctttatc ttcatttaca
      361 agcttccaag taggtggtat agctgtgtat gtttcgatga atgaatcaat tctttcatct
      421 atcgtcttcc cagtatctac ttctgtctca ttactcttag atgaatcgat tctttcatcc
      481 atcgtctgct ccgtaactgc ttctgtctca ttattctcac cggaaactct cttcacaccc
      541 aaacttatat attgtccatc tttagtgtgc atagttaaaa ttcccacgct tttactaata
      601 aattcactat aaccagttct ttcaatagag acttcaatgt atttcacact atcatactca
      661 aaagaaaaat agacatgtaa aagcattttg ttgtttacat ctacataata atcctttgac
      721 tctacttgag accttcctat acggaataca gctttactgt ctgatatctc tataccagca
      781 tccttattat taatctgcca atctccaagt agctcttctg gcaagctagt agtttcttgt
      841 gaacacttta ccacttcact ggtagaggtc gaaacattca cctccgcact gttagaagaa
      901 ttttccaagg aatcaggagc cttagcacaa ccaacaagtc tttatatatt gtgcagccga
      961 tattttcttc attcagtaca cccttgaaat atgcagtgtt agataaagca gttcgtcctg
     1021 atttgctgag gcacccatct cttcttgaac gtactccttg attttcaagg ccgcccgata
     1081 ctctttcgga aaacgctgct tcactgaagc cagcaaaaaa tcatccttct ccggcaactc
     1141 ttccttctta atcattcggg caataaaaaa ctgtaaatgc gtcacaaatc ttaagtaaag
     1201 catcgaatct ttatcaacct ttttttcggg aaacagatat cctagcatag ccaagatatc
     1261 ctttagtgct cttaccgaga gcatggtttc ttccatattt gcttgttcac tttgggcatt
     1321 tacaatgtga aacgcaatgt ttccagcctc ctcttctggc aactgaacat tcattttttt
     1381 gttgatcaat tggatagcat agcagccgat ctcaaattct tttgggtaat acttttgaat
     1441 ttcaatcagc aggcgatttt gaaaaacaat gcctttttga ctgcgttcaa tggcatgctt
     1501 caagtgatca accaaggtaa aaaatagact tttattcagc ttaaagtcaa acttggcgtt
     1561 ggcataatcg ataattcgct tgacgcaaat aattagatca tccggagcag cctcaaacag
     1621 tttaatgtaa tcctttttta gagcatcaga aagtgaatct aatttaaata ctttttggat
     1681 tttgtcagcc ggaaccaaat cgcctttctt tagttgaaat ccaatccctt tccccatcaa
     1741 aattacttct ttgccttgct tatctaagca aagaattaaa ctgttgttta acgttttaat
     1801 tactctcatt agattcaccg ttctctctaa gctaagccat ttcttgcgat tactttttga
     1861 taccaaaaga agctttcttt ttttattcgt tttaattctt taagattctc atcggttcgg
     1921 tcaacatata taaaaccata acgcttattg aagccttgat gggtactaac aacatcaatc
     1981 gctgaccaag gtaaataacc aaaaacatct acaccatctg ttaaagctgc ttgaatagcc
     2041 agaatatgtt tggctagata atcaattcga tcctggtcat taattcgtct atcttcctca
     2101 actatgtcgg gtgccccata gccattttca gtaattaaaa taggtaaatc atagcgatcg
     2161 tacaatttcc tcaatgttaa tcgtagtcca gtagggtcaa ctacccagcc atatggcgtt
     2221 ttatctacat aaggattttc agctgcccgg tagacacctt cttctcccaa catgatctgt
     2281 tgatcccccg ctctagcgga aacatctgat ccgtcatttt tgctggcagc gatggtggca
     2341 gttgaataat aattaatcgc aataaaatcg ggtttaccgg aggataaaat tgcctgatcc
     2401 tctgcctcaa ttttaggata taatccacga tcattcaaat agttttcaaa caaataattg
     2461 tattttcccc gaactgctac atctaagaaa gaccagcctc tcaatgtttc ccaattatgg
     2521 gctgcaattg catcttctgg actggaggta gcttgataca tagccgttaa attgattgct
     2581 ggaccgattt ttcctttaag attcaactga tgatacaagt gaattactct agcttgagct
     2641 aataaaacat ggtgattaat ttgaaagagt tctttttttg aaggcagctc tccacttggt
     2701 ggcaaaccaa tcgctccagg atgaaggatc atcgtgtttt gctcattaat tgtcagccaa
     2761 tagttcacct tgtcaccata ctcagtaagc aatacctgta catagtccac aaaataatcg
     2821 atagttgctc gatttaacca accgccctgc tcctctagaa aataaggtaa atcgaaatgg
     2881 tacatggtga caatcggttc gattccctga ttcaggcatt catcaattac ttgatgataa
     2941 aaagaaattc ccttttgatt ggttttatta ttttcaatat ccataattcg cgtccaagcg
     3001 atggaaaagc gaaaagcttt taagcctaat tctttaaaca gctgtatatc ttctttatag
     3061 tgatggtaaa aatccgaggc aacttcaaaa ttagctaccc cttctgtatg ctgatacttg
     3121 tcaataatag acggttgttt cccatcctcc tgataggcac cctctgcctg gtaggcacta
     3181 atggaggcac cccataaaaa cttctctgga aatgctgaaa gctgtttgta ttgcacaaaa
     3241 atcctctcct taactaattt gcatcaatgg ttcgtgttcc tttaagcttc cgcttgcttc
     3301 actaattcct gcgtaatttt cactgttggt cactaccaca atcactgtat catcgtagcc
     3361 agcgtttcta atggcagcga aatcggcgtt gattaataat tgtcctgctg acacctgatc
     3421 tcctgctttt acaaatactt caaagggttc cccttttaaa ttcacggtgt cgatccccac
     3481 atggatcaat aattcaatac cttctttaga acgtagacca atcgcatgtt tagattctaa
     3541 aatagtaatg acttctccct caacaggtga ataaatgctg ttttcttctg gatgaatggc
     3601 tacgcccttt cccaatagtc ccttagaaaa gacagcatcg ttcacttcag aaataggaca
     3661 cacggttcct tttaccggat tttcaacaat gactgcttcg gctattgccg tttgctctga
     3721 ttcatcagaa gtatcgtcaa atccaattag ataagcaatg ataaaggttg caacaaaggc
     3781 gattacacac gtaataattg cgtgaacaat attcattgga ttttctccaa taaaggctgg
     3841 taaggctgct aagccagggg aaacaaaggc atatctgact aaaccgctca agccagcata
     3901 caaaccagca attgcaccac caatcatgga agcaactaat ggttttttca gcttcaaggt
     3961 tactccatag agtgaaggct cagtaattcc cattaaagca gtgatcccag tggacacagc
     4021 tagctgcttc aactctttgt ttttagtttt cgcggctaca gcaaaggtag cacctgattg
     4081 agcaatatta gaagccagca ttcctggccc attgatcatt tcagaacccg tactgctcaa
     4141 ttgcatagta gcgattggcg tcattgccca agctgtaccc gtaaccacca aaaatggctg
     4201 caaaccaccc atcaaaaatg gaattaacca agctgctttg ccgttaatcc attcagcccc
     4261 cattgctata agatcgttca gcaacgttcc aaacggtcca atgacaacca acgctaaagg
     4321 ccccataatt aaaaatacca gcatcggctt agtaaagaat ttaataaacg aaggcgaaat
     4381 tcgatccgca aagggctcta cataggacat gatccaaaca gtaaagataa tcggtaaaac
     4441 tgatcccgca taatctgcag ctaataccgg aattcctaga aaagaaacgg catcgccagt
     4501 agaaaaaagc tgtgaaatat ttggatgaag caaaatgcca gcaacagtaa tcgccaaaat
     4561 tgggttgaca tcaaacttta tggctgcccc ataggctaat agtactggca taaaataaaa
     4621 ggctgcatca gcaatagtat tcaaaatgat gtaagtttga ctactgtctg atagtacacc
     4681 agtcaaagtc aagacggcca aaatggcttt taccatcccg gcacctgtta tagctggaat
     4741 catcggtgta aaggtggtag aaatgacact aatcaatctt gaaataatac tttcttgatt
     4801 gtctgaagag gggccctttt taattgaagt tcccacttcc ttttgaattt ccttgaaggc
     4861 tgtttggact tcattgccaa tcaccacttg gtattgtcca ccgctatcca ccacactaat
     4921 tacaccaagt gttttttcta aggctttcgt atcggctttc cttttatctt tgagttcaaa
     4981 acgcagtctg gtggcacaat gggttaagct gaccacattt tcttttcccc caacctgttg
     5041 caatacattt cttcctactt ctctgtaatc cattattctc tctccttaaa gcagtaaaaa
     5101 agaccaagtc aaaccacgcc ccaatcaaca ttgaagtgtt agtttgtctt ggtcttgcct
     5161 gcataatcag taacatgcca ataacgttat tttgctgtaa tccaataata gcacattatg
     5221 atagcgcaat caacagggaa tttaattttt tatcttttcg tttgaccgta tagggtaata
     5281 taacagttag atcacatcaa ctagggtata tctgcgtata cttactgcta aaataagtat
     5341 atctaatatt aactatacca gtaaaaccat tcctacataa ccagcgggaa attcccaaaa
     5401 tcgtgaaagc atcaaaaatg catataacgg atcacacaac ttcaacaatt ctgctcttgg
     5461 gaaaaattga tgaatttggt gagtaaagga tggaataaac agcattccaa caatataggc
     5521 aacaatagtg atacttgttt ttttggtaaa aatcagtaag caatagccaa tttcaatcat
     5581 tccgagatat aaaggtatcg atagaatgat ttgggctagt attcttaaaa attaactagc
     5641 aaatgtttaa actgatttat ttctaatcta catgtagtaa gtatttcaat tccaccaatt
     5701 atcaataaaa ataccaaaca tatccataat aattttttct ttttcatttc ttttatattg
     5761 ttataacagt gaataacata aaaattcccg acaatcattg taatattttt ttgtcacctg
     5821 tattagtatt taatttatag gaaactataa aaactcccta gtgaatttac acctttggga
     5881 gttcctgttt ggacggtatg cataatacat ttaaatcaat ttaacattaa cacctcagca
     5941 tatggtatct ccacatatat acggtctttc tttggatagt actcaatatt tatgttgata
     6001 gcacttccat tgtctaatat agctttagcc cggtatgtgt tatctccatt ttcatgagta
     6061 atcgtcacat catcacatga tcgatttaag ttgtactgtt cttgtaaaat ctgtgttacc
     6121 aatggtttcg atgacttctc taatccagat gtacctgtca tttttgaaaa aatgatacct
     6181 ataatagcca agacaacgat aactataatg ataaacttct tgttcatatt tactttattc
     6241 aactttttat ctcctatctc caaaataatt tgctcttatc cctcttatca gtacattgac
     6301 atccctaaaa gtacaccaaa gattgctcca gctacccctg ctatcatcaa aatcgtaccc
     6361 atttgttcat ctttatctcg atataagtag cccataataa cagcaacagc accaaataca
     6421 acaggaataa atgctaacgc tactaccata gaaatccaac caataatcaa atacagcttc
     6481 cctgtctctt gtccatgtac gatttgttcc ttcatttcat taattgtctg ctcagtactt
     6541 ttctttactg cgtctgctcc agttgtctca tctgtttctt gtagtgctcc gcaaaactgg
     6601 caatacttac tttcatctgt tatttctttg ccacattttc tacaatacat gtcttctcct
     6661 cctaaaaaca ttcaattgtt tattagttaa ctgtttactt tattaaatac atcagaaaac
     6721 agctcattca ttggtacact taatatctca gaaagcatct tcatttgata gggtttagga
     6781 atcgttctcc ctgtttgcca cgaacttaca cattgctgtg taacattcat ttttttgcta
     6841 actccgtttg agaaagtcct ttttcttttc tgtatcggct gagattactt acttttttca
     6901 tgattctccc ctcctgtttt attatataca acaatatgat gtatttcaac tcataaaata
     6961 taaaaaaact tcaaatacca ttacaaatac aacaacttgt tgtactatac tgagatgggg
     7021 gtgtattaat gaaacagaaa caatatgcaa aggtcattag agaaaagcga aaagctctcg
     7081 ggtggacaca aaaagaatta gctgaaaaaa tcttctctac acaacaggca gtagcaagat
     7141 gggaaaattc tgtaacagag cctaacctcg atagcttaac cgctctttct agagccctag
     7201 gaacccctgt aagccatttt ctagacaatg ttgtcgtaga ttatgaggaa gagtttttgg
     7261 ctctctatcg ttctcttagc actgaagatg cagttcgaac aattgactat atgaagctat
     7321 taaaaaggca agaaaatgaa cgcaatcagc tattgaaaga ttgaaaaata aataacccac
     7381 cgaactttaa cggtgggtgt attgagatga aataagatta agagctatgg ataacctgtc
     7441 tagtttaata caattgggtc ttttctcttc cttactctcc gtccatcatc tcttgttaca
     7501 cttgtaaatt gataaagagg aactaattca actcctgata atatcaattc ttttattact
     7561 tcatctatcc ctgctccttt tgctaggtag actaaaaaac tactacttgc tttcacattg
     7621 tatagtctct taatatcctt agaagctcgt tcaagagtga atccttgttt aacaaagtag
     7681 cctctgtttt cttttaaagc attttccacg ctgttattcc caacattacg tagacgttca
     7741 caaacagtgt tagtaagttc tttctctgtc attagatatg ttccttgcca cgataagccc
     7801 tgtaaaactt catgtgcttc atctaaaatc aattgttttt ccttgctaag ttctgatcgg
     7861 gaattttgag gaaatactct tttagcctct ttttcgttaa aacatatctt aacccattca
     7921 taagaaaaat attgatatgt aaaatgaaat ttttccagaa gtggaaggat atcttcattc
     7981 aatttttgaa tcacagaagt tgcattaaaa acgtctaatc gatagacatt gcttctctta
     8041 tgattaaagt ttcctaacgc tttctcttcc tttaacctat ttaggtattc gagcatcttc
     8101 aaaggaacat cttcatcatc taattttttt atcaatccta taaaagtcat aagtgatagt
     8161 aacttattac atttatctat gtcataagtt ttaccatagt aaaaactcat ttttttagct
     8221 aattgttccc ccgacaaaat acaagtcaca ctttccttac tatcatcaaa aaaattaaca
     8281 tcagaaagca agacatcata gacacgtcgc atttcctcta gataattcct tattgtcctg
     8341 aaaagtttgt ctttcattga ttcaagagtg tcaaaaagta cttttgattc ctctttcaat
     8401 tttcgaaaat gtgtaggata caagtctagg ttctctaata gccaattaaa tgtttctacc
     8461 cgactgtctt tctttaaaat tagcatcaca actccaataa aattttcact gatatcacaa
     8521 ttactggaat gacaataaaa aatggaatgc ccagttttac tggtaaaaat actagcagag
     8581 ggattatgat cgttgtgaaa cagacatctt aagtttttac ttgacttaag taatttattc
     8641 atatcaatgg ttaacaatct ttcataaatc tcattaaaag acatgtcgga ggaatcaaac
     8701 aggcagtctt taaaccactg tttcatttct tctttgtcgt cattttttat caacttaaca
     8761 aaatcggttt cattgactag ttcatactct ctccctgaat cactttgaca atttttagaa
     8821 catgtcatgg taaaatcaat cggaagctca gtaatatcta acatgttgtc ttcattaatt
     8881 attatcactg catctgactt atatcctcca aaaaacattc tattcgaagc atttacattg
     8941 tcatcacacc cggggatgtt ctttttcaag tattcgtaaa cagcaaccat tttaccatag
     9001 tcagtaattg cttcttttaa tagaaagaca atcttgtatt tttctagatt ttcagagtac
     9061 cgaatacttt tttgtatcgc aaacgcagaa ttatgaacta aattgataga taaaaccttt
     9121 tctatgctta agtatctact atcatcttta ctcaaatcta ctaccttccc cttctcatct
     9181 ttaacagtgt tatcgacatc cagaataatt actctggttt ccacaaaact ctccttgtca
     9241 ttgcctttat tctttgtctt gcttggaata aatgattgtg atgaatttag tatctctaca
     9301 aactcatctc ttgtaacttc tctatagtta tctaaacacc tatctcttac atcagttttt
     9361 tctttttttg taactggctt ctctaataat ttttctttat ctacataaat tggaatatta
     9421 atcattgatg tttccatttg tgtttcctcc ttataaaatt ctgttaaaga ctaaaacata
     9481 cagataataa aaacaaactc tttattacta tatgttttag tcttatttgt ttactcaacc
     9541 tttataccgt tccatgaaac tatcaatatc agatttttta aatctaatga ccgtatcaat
     9601 ttttataaca ggtaacccgt attgagaaac atattttcta atcgttccta ctgatacacc
     9661 tagatattca cttgcctgct gatagtccat atactctaga gtgactccag aaataatgtc
     9721 ttctagcata ggctttagca ctgtaattat ctctttcact tgttgttcaa cttgtataga
     9781 aacaatgcta tcaatgattt gtagagacat atttccccct cctttcgtga ttgcttgaac
     9841 aaattttcta agaattatct tcgtattttt tgctcaaaat aaaagtattt tgttattaac
     9901 cgattaatca aacaaaaaat gattaccagt tcatcgacat acacataata cctcgataaa
     9961 aaagaggtgt caatatcttt gtaaaattta tttacaacac ccctttttcg aggtatacta
    10021 attttaggag gtgtggtcaa tatgataaaa cgtccaccaa taaattattt agaacgaaaa
    10081 aagatactcg gaacaaaaat aaaagctata agaaagagta aaaaattaac acaaccagca
    10141 tttggtctta tgattaacaa cggtcaatta attgacaaaa aaactatcta cgaatgggag
    10201 aagggaacct atttaccaat accagagcga ttatcccgaa ttgctgattt gggaaatatg
    10261 agtatagagg agttagtttg tggcaatgta gaagaatata ttttgggaat tattctttat
    10321 agagatagca ttgtactaga tggtataaca tttccagata aaaacctgtt ccagcatctt
    10381 cgtcaacagt tcccaccagt acactctaat ttagatacat ggctggatag atattccaaa
    10441 ttagaaccag agatgcaaga atttattgct aacaagactt gtaataaagt taaaaatgaa
    10501 aaaattagtc ttttcaatat cttaaaaata gaagaattat tcatcaatgc tattgtagaa
    10561 gaatttgaca ataacattct tttcctcact agcagtatag aagaactact agaaagaatg
    10621 gtagatgaat ggctacctat tcaattgaaa gacatgtctt atcccgaaga agctgtgaga
    10681 gaaattactg ataatataaa caagctagaa caaacgattt catcaattgg gaaaaaatac
    10741 actaagaaaa aaatgaaggg aggtgatacc atctagtggt aagtactcct tgtaaactaa
    10801 gaggagatga tttcatggca aaaatatcaa agtacaaaaa gaaaaacggt caagtggcgt
    10861 ggatgtataa aggacacatt gccaccgatc cacgaactgg tgaaaaaata aatactacga
    10921 gaagaggttt tagtagcaaa caagaagctc aaaaagatta tgacgagtac agacatcaaa
    10981 tattatacgg tgttaaaaag aaagcacctg atatgacatt tgaagacctg tataatgaat
    11041 ggataactca tcagcgtacc agtgttaaag cttcaacaat cgcaatctca gtaagatacg
    11101 caaataatca aatacttcca gcatttggta agcttaaact atcgaatatc tctgtcccat
    11161 attgtcaaaa agtggtagat gaatggcaca gtaaatatga aagttacgat tatatgagaa
    11221 aacagactgc acaaatttta cgctacgggg ttgcaatgca atatattgac aataatccta
    11281 tggaaaaaac acttcttcca cgaaaaaaag agtatgagaa aaatcgtaaa ttttactcaa
    11341 aagaggagct taataattta ctggatgctt ttaaagattt tggtaacatg aaacaatacg
    11401 ccttcttcag attattggca tacacaggta tgagaaagag tgaagtactc gctttacagt
    11461 ggaaagacat agacacgttt aataaagaac tacatgtcaa taaaactctt gctgtagatg
    11521 aatttggtaa agttataatt caatctccga aaacaagagc ttctagacga gttatctctt
    11581 tagatactga gactctttct atcctcaaca actggaaatt acagcaaaaa gaagagtatt
    11641 taaaacttgg atacaatact agctctaaag agcaacatgt ttttacaaca gttaaaaaca
    11701 ctctatacat tccaaacact gttaatgatt ggttaagata tattttgaaa aaatataact
    11761 tgcctcgtat tacacctcat gggtttagac atacacatgc tagtttatta ttagaagcag
    11821 gagaatctgt caaggtagtt caacaacgat tagggcatga aaatagtaaa gtgacattag
    11881 acatctatgc acatattaca aataatgcac ctaagaaaac aggtcaagac tttgctgata
    11941 tgatggctca tcagtaaaag agctggtcaa aatctggtca tttctagtat acaggcaaaa
    12001 caaaaaagct agaaccctta tatat
//
LOCUS       DAZKNH010000080        11991 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000080 DAZKNH010000000
VERSION     DAZKNH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.080"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(113..2188)
                     /gene="glyS"
                     /locus_tag="ACG8MG_002047"
     CDS             complement(113..2188)
                     /gene="glyS"
                     /locus_tag="ACG8MG_002047"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFQ0376072.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2190..3107)
                     /gene="glyQ"
                     /locus_tag="ACG8MG_002048"
     CDS             complement(2190..3107)
                     /gene="glyQ"
                     /locus_tag="ACG8MG_002048"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0376073.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3493..4311)
                     /gene="recO"
                     /locus_tag="ACG8MG_002049"
     CDS             complement(3493..4311)
                     /gene="recO"
                     /locus_tag="ACG8MG_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFQ0376074.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4454..5353)
                     /gene="era"
                     /locus_tag="ACG8MG_002050"
     CDS             complement(4454..5353)
                     /gene="era"
                     /locus_tag="ACG8MG_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFQ0376075.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5368..5763)
                     /locus_tag="ACG8MG_002051"
     CDS             complement(5368..5763)
                     /locus_tag="ACG8MG_002051"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0376076.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5747..6223)
                     /gene="ybeY"
                     /locus_tag="ACG8MG_002052"
     CDS             complement(5747..6223)
                     /gene="ybeY"
                     /locus_tag="ACG8MG_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFQ0376077.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6252..8444)
                     /locus_tag="ACG8MG_002053"
     CDS             complement(6252..8444)
                     /locus_tag="ACG8MG_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFQ0376078.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8468..9439)
                     /locus_tag="ACG8MG_002054"
     CDS             complement(8468..9439)
                     /locus_tag="ACG8MG_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFQ0376079.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10144..11523
                     /gene="brnQ"
                     /locus_tag="ACG8MG_002055"
     CDS             10144..11523
                     /gene="brnQ"
                     /locus_tag="ACG8MG_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0376080.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            11805..>11991
                     /locus_tag="ACG8MG_002056"
     CDS             11805..>11991
                     /locus_tag="ACG8MG_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0376081.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIK"
BASE COUNT         3277 a         2553 c         2191 g         3970 t
ORIGIN      
        1 aaagaataaa ataacaaaaa ttattaaaat agaattaata acagctattt ataacaaaaa
       61 aaacagagac acagcacgtt tcgtgcatgc tctctgtttt tcaaacaaaa gactattttg
      121 tgatgatttg atccaagctt gctaatgaca aagccatggc agctatttga cttagttgtg
      181 ttagtcggtt cttacgtact gctggttcat cagccatgac catcgtttga tcaaaataat
      241 tttcgatcag tggacgaaga gcgacaagct ggtcgtagtt ttcgtggatc gtttggtcag
      301 caaaacttgc tttcattgct tctactgctt tgtgtaattc tttctctgcg tcgttttcga
      361 acagctcagg gtctacttct gattgttttt caagctgcgc ttttttcgct aagttcacga
      421 cacgtgtcaa agcttccatt gaaggtttga agtcttcatc tgcaagatgt tgtttgaaca
      481 attgtgctgc tgcaaatact tttgtcagat ctttttgttc cgcattcaaa acagcatcga
      541 tgacatcatg acgaatattc ttcgttgtca ataattggcg cagacggcct ttgataaagt
      601 cgatcacttg ttgttgccct tctgataaac caatgccgaa acgatccgta tctttactga
      661 taacatcgct aatcgtttct tgcagaacag acaaaggaaa ggcccatttt ttcgattcga
      721 tgatccgaac gacaccgtat gcttggcgac gtaaggcata aggatcgttt gacccgcttg
      781 gaatcatccc aactgcaaag aaagaaaaga tgctatctaa tttatctgca atcgctaaaa
      841 cagaacctac tgtagactga ggtagctctc cttcactgga tataggcatg taatgctcac
      901 gaatcgctgt cgctacagca ggtttctcgc ctttcagaag agcatatttt tcgcccatga
      961 tcccttgtag ttcaggaaat tcccctacca tgttggttac tagatcaaat ttatagattt
     1021 cagcagctcg ttgcaaatcc atcaattctt cctcagatag tcctacttgt tcgccaatga
     1081 tacgggcaat gacttgtacc cgttgcattt tctcatagat actaccgatt ttttcatgga
     1141 aagtaacagt ttttagtttt tcaacgtatt ggtcgattga agctttcttg tcttcattat
     1201 aaaagaattc cgcatcttct aaacgagccg tcaatacttt ttgattgcct cgaaccacat
     1261 cttccaattt tacccgatca ccattacgag cagaaataaa atgaggaagc agcatacctt
     1321 gatcattccg tacctcgaaa taacgttgat gttctttcat agaagtgact agtacttctt
     1381 caggaacaga aagatatttt tcttcgaagt cgccagaaaa gacagttgga tattcgacta
     1441 aattgttgac ttcttctagt aaatcttcgt ctagatcaat aacccagtga tttttttctg
     1501 ctaattcgtt tgcttgtgta acgatcattt cttgtctttt ttgcggagac gcaatgacga
     1561 attgttcatt caatttttct tcatattctt ttgcatgcgc aaatgtcaca tcttcgccta
     1621 agaaacgatg acctcgagag gctctgcctg ttttgacatc caagatttcg aatgggatta
     1681 cttcgtcatc taacaatgcc acgatccagt gaatagggcg gatatattca aaatcgtatt
     1741 ctgcccaatg catcgttaca gggaaagtca aacttttgat cacccctgct aagcctgtca
     1801 atacttcttt tgcttcctta ccatgagtgt atttcgttac atagacatat tccacacctt
     1861 ttaattctct aaaagtgata gcgtctgtcg tcagaccttg tccgcggaca aatccttccg
     1921 ctgctttcgt ccagtttcca tctgcgtcca aagcaatttt tttggctggt cctttgactt
     1981 cttcttcact gtccgcttgt cgttctggaa tatttgttac tttgattgct aaacggcgcg
     2041 gtgtggaaaa tgtttcgatc gtatcataag tcaattggtt ttcatctaaa aattttgctg
     2101 ttttttcttc taattgtttc atgcttggtg tgacaacatg ggcaggcatt tcttccagcc
     2161 cgatttcaag taataagtct tttgccatct tattcagcct ccttcacggt ttctggattt
     2221 gctgtgtttt tattcaacaa agggaatcct aatttttcgc gttctgccac aaagattttg
     2281 gctacagcac gagccatgtt gcggatgcga gaaagataac cggcacgctc tgttacggat
     2341 actgcaccgc gggcgtctaa taagttgaat gtatggctac atttcaagat ataatcatac
     2401 gccggatgaa caagattttc atcgatacag cgtttagctt ctttttcaaa acgatcaaag
     2461 ttgcttagta gcaagtcttg gtcgcttact tcaaaagaat atttggaatg ttcatattct
     2521 ggttgtttga agatctcgcc atatttcacg ccatcagccc attctaaatc atagacactt
     2581 tcaacttctt ggatataaga agcaagacgt tctaatccat aagtgatctc agaagttact
     2641 ggtttacaag gcagtccacc aacttgctgg aagtacgtga attgtgtgat ttccattccg
     2701 tcaagccata cttcccaacc cacaccggca cagcccatgg atgggttctc ccagttgtct
     2761 tcaacaaaac ggatgtcgtg ttctaatgga tcgattccta aaaggcgcaa gctttctaaa
     2821 taaagctctt gaatattctc aggtgaaggt ttcatcacca cttggaattg atgatgctga
     2881 tacaaacggt ttgggttttc accataacgt ccatcagctg gtcggcgaga tggctctacg
     2941 taagcagcat tccatggttc tggtccgatt gcccgtaaaa aggtatatgg gctcatcgta
     3001 cctgctcctt tttctgtgtc ataagcttgc atcagcatgc agccgtttga tgaccaaaat
     3061 ttttgtaatg ttaaaatcat atcttggacg gtcaattttt tactcatctg ttttgcccct
     3121 ttcaatttta aagcgttgct gtcaaataag aatacaggaa agaaaacgac aaaaaatccc
     3181 tatgtctgat tacagacata gggacgaata attcgcggtt ccaccctact tccgaataaa
     3241 gattcggcag cttcatttat cgtactcgca agtgcctctt caaaaatcgt ttgggtccgg
     3301 ctcccactct cccgaactcg ctaaaccacg tgttttttac tgatcttggt caatgtacgt
     3361 attcattttg cagttttctt gtttctataa tagatcctat cactgaacga tctatttaat
     3421 ctatggttaa taatagacgg aactccctgt gtttgtcaaa tggttttgat tttttgttgt
     3481 atgttttatt tactattctt ctttttcagg tgtctctttt tctttttcag acatgtcttt
     3541 ctctgacgtc tttaatacgt gtgcccaaga cttcatttga ttgatgaact ttttgctttt
     3601 caaatggata ccgacatatt catcatacag ctggtcgatg agttcacgga ttgcttgttt
     3661 ggtttcttct ttcagatgga ttccactaat tttatcataa gaaatagctg aaaacatccg
     3721 aacgaaataa acagctcgag gatctgcatg ataacggtga tcgtccatcg accaatgtct
     3781 ttcgcatatc acaccaccat atttggaaga atagtcaaat tttccttttg tttccccgca
     3841 tacgacgcag tgtgtccaga ttggtgcaat tccgaagcgt tgcagtattt ggacttcaaa
     3901 aatattcgtg atgatttctg catcgtctcc ttggtccatc cgttgcagtg cttgttgcgt
     3961 aaactggaac aaatgcgggt cgtactgatg atcttcgatt gccgcatcta ctagattcaa
     4021 taaatatgtg ccatagccgc tcaaaaagat gtcttcttgg atgtgtcgaa acgcatggac
     4081 atctttgaca ctgcttaaaa atgacaaacc ttcacttcga aaattcccga tataaactgc
     4141 ttcagtaaaa gggagcaaag ccggagctaa gggctgattt tgccgatgga tctctttgat
     4201 ataaaacatc attttgccag aagattctgt aaagattttg actaatttat ctttttcttt
     4261 gtaattacgg ctagataaaa tcaagccttt tgattctgca agctggctca tattctccct
     4321 cctaaaaaat aaatctggaa aagcagattc tgccgatcca gatcatttat gttgcgatct
     4381 tccgcagctt ttctcaacac gcggattttt attcagttta tttctatacc taatttacaa
     4441 gaagctcaga atatcagtat tcgtcttttc ggtagccgaa gtcttgcaga tagacttttt
     4501 tgtctcgcca gtctttttga actttgaccc aaagttctag aaagactttg tctcctaaaa
     4561 gattttcgat atcttttctt gctttcgttc ctacttgctt cagcattttt ccgcctttgc
     4621 cgataatgat ccctttttgg ctatctcttt cgacaataat cgtagcctgg atatggattt
     4681 tatcattctc gtttcgcttc atcgaatcaa caacgactgc gacagaatga ggcacttcgt
     4741 ctcgtgttaa aaacaagact ttctcacgga tcaattcaga aacgatgaag tattcaggat
     4801 gatcagtcac ttgatcatct ggaaaatatt gtgggccctc tggcatttca ctgactaata
     4861 catccatcaa acgttcgacg ttgtttcctt ctgtagcaga gattggaatc acttgtgcaa
     4921 aaggcatctg cgttgaataa tcttcgatga ttcccaacaa ctcgtctgga tggattttgt
     4981 cgattttatt gataatcaga taaacaggag aatgcacgtt ttttagacgt tcaataataa
     5041 aatcgtctcc ccgaccgcgt ttttgatcag cactaatcat aaataagacc gcgtccactt
     5101 ctttcaacgc actgtaagct gcttctacca taaaatctcc taaacgatgc tttggtttat
     5161 ggattcctgg tgtatcaata aaaatcaact gagcttctgg tgtagtgtat actccttgga
     5221 ttttatttct agttgtctgt gccttgtcac tcatgatggc aattttttga cccacgattc
     5281 gattcaataa tgtggatttc cctacatttg ggcggccgac gattgcgaca aaccctgatt
     5341 tatgttctgg catatatgtt ctccttttta attaaacaac aaatgatata ttttagggac
     5401 aaataaaatc aacccaacga tgacggtaaa acaagcggtc actaaaactg ctccagctgc
     5461 catatctttg atttttttcc caatcggatg aaaatgaaaa tccgtaaaca tgtccacgac
     5521 attttcaaaa acagtattga tgatctctac tatccatacg agaaaaacag ctaaaagcag
     5581 ccataaccat tctatccgat caagttggaa aatcacgccc ataaacaaag cgaggattcc
     5641 gaacgcaaca tgtttcctca tgttgcgttc ttcctcaaaa acagttttga ctccttggac
     5701 ggcaaattcc acggacatca ggaaatgttt gtttttctct acgtttttat cttttaagtc
     5761 cataggcatc taagatctct ttctgtaaac caaacatttc tttctcatcc tctggtgtca
     5821 tatggtcata cccgttgata tgcaagaatc catgaaccgc taaaaagcca agttcccgat
     5881 catacgaatg gccgtaagct tctgcttgct ctgcagcacg ttcagtagag atcatgatgt
     5941 ctcccagtac acgaggcaat ggattctctc catcttcaaa aatcactggg atttcgtctt
     6001 cgccctcttc ttccaaggca aaactgatga cgtctgtcgg tgcatctttt ccacggtaat
     6061 cacgattgat tacttggatt gctgcattgt ccatgaaagt aacagacatt tctgtttctt
     6121 ctggcaactc taaatatgat gctgcaaatt gcagcaagtc gtcgatttct ttcattttct
     6181 cttctgagac ttggtttgtt tcatccatga aggtaatatc cattatacat cgcttctttc
     6241 tattcttctt gctattcttc ttgttcttct tttatcttct caacttccga tttcatttta
     6301 ggatatttga tccgagaatg gaaggtactg cttaaaccgc tgatcaatga tttttctact
     6361 ttacggattt cttttaacgt caatccactg tcgtccaatt gtccatcgga aatacgctct
     6421 tcaataagat tatggacaaa actttcgatt ttttcgctcg ttggatggtc cattgcccgt
     6481 actgccgctt cacaactgtc agctatattg acgattcctg cttcacggct ttgaggtttt
     6541 ggtccaggat aacggaattg ctcttccgta acttccgggt tgcgttcctt tgccttaacg
     6601 tagaaataac gcatcagcgt ggttccatga tgctgtctac aaatatcgat caccatttgc
     6661 ggcatattat aatcttccaa tattttggca ccatctgtca catgtccaaa gataatatgt
     6721 ttactgtctt ctggcaataa gaaattgtgc ggattttccg ctcctgaagg caagttctca
     6781 acgaaaaagc tggcgtgttt gatcttgccg atatcatgat aatagcaagc tactcgagtt
     6841 agtaaagaac gtccaccgat ttctgctaca gcattagcac ttagattggc taccatcatg
     6901 ctatggtgat acgtacctgg tgcttcttcc agcaactgtt tcaataaagg atgattggga
     6961 ttgctgagtt catttaatac gatcacacta tcatcattga ccatcaattc aatatagggg
     7021 tgcagaccta tcgtcagcaa gaatgaaaac agtgcacctg tgaatccgca gactagcgtt
     7081 aaccaagtcg ttgcatcccc aaagctcatt ccttggtaga taatcaaaat gacatccatc
     7141 atcacaggaa aaataatgac ccacatagca gcggataacc cttgttctga gatccgtttg
     7201 cgtctgacga tcgttgccat gagaccagaa aatacatagg acatcaaaat aactgtcaga
     7261 aagttcgtgc caatcgaatc ataaaaaatg aaaagtgcgg atactgcttg aaaaatacca
     7321 gccatgatcc ctgctcttcg attcaagaaa aagcttaaaa caagtggagc aaaagccgcg
     7381 gggtaaaaca gcggaatata agcagcttgt tctgtttgga agagttggaa aaatttcatt
     7441 agtacgacgc ttactgacat tgccgtcacg tagaaaagta cgtattttgt tctttctcct
     7501 gcattttgga attgaagtga attataaatc agtacaccga tttgaagcaa aacagctaaa
     7561 accatggcaa tgagtgggaa aatagaagtc gtttgattcg tcaatcctag taattccaat
     7621 tttttcatcg ctgaagcatc aatttgattt ccttcgcgaa caataatttc tccttggaag
     7681 atcatcactg gttgtacagc ttctcgtgca gattgtttta attcttctgt ctttttctca
     7741 ttcaatacat cattaactac gatgccttga tccactaagt agcgaatcat ttgttgctgc
     7801 tctgttgtga cattcaaata ttgaatctga tcttctgctt cttgtttaaa agcttccaat
     7861 tcgctttcac gaacttgttt actcatctgt tcgtcaatga gcttcaaact ttcatcccta
     7921 actgtgtgaa tttccgtttc agtcatttca aatatcgtcg aataaaaatt gttcggcagt
     7981 ttttggtaaa acgcaacgtt ttctgcattc acgttttcaa aatttttctt cagtgtagca
     8041 atccgctctt ctgtagtagg tttcgggata ttttcatctt ccttcgcctt atctaccttg
     8101 ccattataag atttgttgat ggtatcattc gtcttatcga tcaattcgaa taattgcttg
     8161 atccgattgt tctggtcatc tgccagatct ttttgataag tatattctgg agtcactgct
     8221 tctgctgcca gttttcttct ttgttcggtt tcttccgtgt tctcaatcgt tttattcgca
     8281 cggatactct cttctgctac ttggccttct ctgaaatcaa tggccttttg tttgacacta
     8341 ctaaatgtaa cgccgcagac aatgataaaa aacaagaaca gaaggaaagg caatgctgcc
     8401 tttccgagtt tttcttgtag tttctgcagt gttttattca acatgtgctt atcctcctaa
     8461 gcaaagctta ttcttgatga agatccgcct tttcataagc tttgatgatt tcggcaacta
     8521 cagggtgccg aacaacgtca cttgcttcaa aattaacaaa atcgattttt ttgatttgct
     8581 ttaatgttcg ttctgcatga acaagaccac ttgtcgtccc acgaggcaag tctatctgac
     8641 ttgtatcccc attgacaatc atttttgatt gataaccgag acgggtcaag aacattttca
     8701 tctgagcgac tgtcgtgttt tgtgcttcgt ccaagataac gaatgcatca tccaatgtcc
     8761 gcccccgcat gtaagctaaa ggtgcgattt cgatcacgcc tcgttccatc aatcggttcg
     8821 tatgatccaa tccaaaaatt tggtataaag catcataaac aggccgcaga taaggatcca
     8881 ctttttcttt cagatccccg ggtagaaagc ctaggttttc ccctgcctcc acagcgggcc
     8941 gcgtcaaaat gattttttgc acttcgcctt tttttagggc agcaattgct aatactacgg
     9001 caagaaacgt tttccctgtt ccggctggcc ctacaccaaa tacgatgtca tgtttggcta
     9061 ccgattcaac gtatttcttt tgaccgctgt tttttacccg tataggtttt ccattacggt
     9121 ctttgacgat ctcttcctca tacatctcaa caaaataatc taatgtacct ttttgcccca
     9181 ttttcaatgc cgtcacaaca tctggcgtac ttacgtggat tcctcgtttg atcaattcct
     9241 gtaatgtccg aatgactgag gcagccaagg aaatctggga ttgctcacca atgatctgaa
     9301 tgatttctcc acgtgtatgg atggttgttt ccgttgtttc ctcgatcagt tttatatgct
     9361 tgtcgtgtgc acctaaaagc atgctgacat catcagcagc tgtcaatcta atctctaaag
     9421 aactagactc ttccgtcaaa aatagacatc cccttttttt cgattcattc tttcctattt
     9481 taccatactc agaagaaact atatagcaaa taagaaaagg ttgtaaaaga gtcgcttaac
     9541 catacgtacc aagaaacgtt aaaaaatagc cgtttccatt cgctaaaggt ctcgttttga
     9601 taaaaagaag ctgagaatgt aagttatatg aaagtggaga acaaaatatt tcgtttcatt
     9661 caacaagtat tctctaggta tttttcctgt ttcaaaaaaa gaaaacaaaa tagttaaaat
     9721 attcttgcat ttcaaaaaaa acaggtgtat tattacgtca acaaataaac atacagcaaa
     9781 gcgtcgagga aaacagtagg ctattcaacg atttattccc agtgagtcat gtaagtgaga
     9841 aatgacaata aatcgaatag acgaacatca tttccgagtc aatgtcgaaa acgtagatca
     9901 ttgtggctgc ctccattatc aggctagcgg ataactttat caaaaagcgt ccgtgaatga
     9961 agaagctttt ttaagcgaaa aaaggtggta ccgttctaga atctagagcc cttttcccaa
    10021 gtggaaaagg gctttttttt attttaaggt ggtggttttc atggaatctg atattagtgg
    10081 cattactaat tgctgcaacg tttatatcgt caatcaaaat gataattatt ggaggaaaaa
    10141 tttatggaag aaacaaaaac ttattcatgg aaacaactat tgatggtcag ttcacttatc
    10201 ttcgggatgt tcttcgggtc aggtaatcta atctttcctg tccacttagg tcaaatggct
    10261 ggcgcaaact gggtcagtgc tggcgtagga tttgctatct caggtgctct tttcccatta
    10321 ctagctatct tagctgtagt agtgacaaaa agtgatggtc tgtatgactt agctaaacct
    10381 gtcggaaaat ggtatgctgc cttgttctta atccttgttc atttgacgat cggaccattt
    10441 ttcggtacac cacggacagc tgctactgct tttgaaatgg ctgctaaacc atttttgcct
    10501 gaaaaatata attcattagg tatgttttta ttctctgctc ttttcttttt acttgcttat
    10561 ttccttactg tccatccaac acggttggtc aaatacgtcg gaaagtatct aaactccttg
    10621 tttcttgcat tattagcaat catttttgtc gtttctttat tccatccaat gggaaatcta
    10681 aatcaatctg ctgcagatac ttatcaaaca aacgctttaa caaatggtgt cctagaaggt
    10741 tataacacgg ttgatgcagt agctttgctt gccttaagtg tcacttttgt ccatgcagtc
    10801 cgtgaccttg gtttcaaaga taaaaaagtc acagaattaa cggcaaaagc tggtagcctg
    10861 agcgttgcgc tagaagtcat gatctatttt ggacttgttc ttctcggtgc gatgagtttg
    10921 aataagatgt ctttatcaga aaatggcgga actgcacttt ctcagatcac taatttttat
    10981 ttaggtaact tcggcgctag cttcttagga gtgatggtca cattaggtgt cttcactacc
    11041 gctatgggac tcgttgtttc ttttgcgcaa gatttccata aactgttccc taaagtcagt
    11101 tatatgacat ggctgcggtt aacgactttc gtctcttttg tcgttgcaaa tgctggttta
    11161 gacaatatca tccaatggtc tttaccagta ttgatgcttt tgtatccatt gtcgcttgcg
    11221 ttgatcttgc tttccttaac agcaaaattt ttccaaaaga caccatttgt ctatcaagta
    11281 acgatgttgt ttgctgctgt tcctgccgta ttggatatgc tggcaaattc gcctgcttta
    11341 gtgagtcaac aaagagttgt agcaagcatg ctcgaatttt atcatcatca cgttcctttt
    11401 gctgcattag gacttggctg gatggtccca acgttacttg gttacgctgg cagcctactg
    11461 ttttattacg cctatcgcct atcagggtat aaacaagaag caaatgaact accagaagaa
    11521 taaccaaatc aaacacaatt taacataaga aatattaaac aggaaaaaac cagctagatg
    11581 aaagaatcct attctttcgt ctagctattt tttagttctc ttttttacgc gcacaaaaaa
    11641 actgcggaca atgattgtcc gcagtttaaa tgggctctat aatatctagt gttggtgacg
    11701 ccttctgttg ccaatacttt ttcttttttc taataaacgc aaacagacac cttgaaatcc
    11761 tttagaataa agtcgacgaa aaccattcaa aggagtgttt caagatgtcc tattcagagt
    11821 ttaccaaaga gttactagat attctagacc taaatcttat ctttcatgaa gatgcctttc
    11881 gcaaagagcg aatcaatgac gaaacatgtt ttgtttttga tggcacattg acctatcaac
    11941 cagaggaatg cttccattgt cactatcaaa ataagcaaac gatcattaaa t
//
LOCUS       DAZKNH010000081        11922 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000081 DAZKNH010000000
VERSION     DAZKNH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.081"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..2055
                     /locus_tag="ACG8MG_002057"
     CDS             37..2055
                     /locus_tag="ACG8MG_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0376082.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    2125..2331
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2482..4896
                     /gene="leuS"
                     /locus_tag="ACG8MG_002058"
     CDS             2482..4896
                     /gene="leuS"
                     /locus_tag="ACG8MG_002058"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFQ0376083.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5244..6797
                     /locus_tag="ACG8MG_002059"
     CDS             5244..6797
                     /locus_tag="ACG8MG_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFQ0376084.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7178..8962)
                     /locus_tag="ACG8MG_002060"
     CDS             complement(7178..8962)
                     /locus_tag="ACG8MG_002060"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0376085.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8977..10698)
                     /locus_tag="ACG8MG_002061"
     CDS             complement(8977..10698)
                     /locus_tag="ACG8MG_002061"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0376086.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10798..11559
                     /locus_tag="ACG8MG_002062"
     CDS             10798..11559
                     /locus_tag="ACG8MG_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0376087.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3902 a         2159 c         2421 g         3440 t
ORIGIN      
        1 attaataata gataaattga agttggagga tagaagatgg aaaacattgc aagaaaagaa
       61 cggagacgtt tagaacagac caaacgtttt cgaaaagcaa aacgaggggc agcaatcgtg
      121 ggaactgcga tggtaggctg ttctgtcgct gccccattag tacagccggt tcctgttcaa
      181 gcggatgaaa cacctgttca gttcggcgca agaatcaata cagcggcatt tatcacagaa
      241 attgctactt atgctcaacc aatcgctcaa gccaacgatc tatatgcatc agtgatgatc
      301 gcacaagcag ttgtagaaag cggttggggc agtagtgcgt tatctcaagc accttattat
      361 aatctttttg ggataaaagg cagctatcaa ggacaaaccg tctacatgga tacgcttgaa
      421 tttttaaata atcaatgggt aaataaaaag gagcctttcc gacaatatcc ttcttttgct
      481 gaatctttca atgacaatgc ctatgtactt agaaatactt catttggcaa cggttattac
      541 tatgccggta cttggaagag caacacgaaa tcctatacag atgcgacagc ttgtttgaca
      601 gggcgttatg cgactgatcc tggttacgca ggcaaattaa ataatatcat cgcaacttac
      661 ggattgaccc aatatgatac accagctagc ggaaacgcag gcggtggagc gactacaggt
      721 ggcaataata ctggaagtgc aggtagtact ggcggttcaa gcaattctgg taatacaaac
      781 tcaggttcag caacttcgac cacttatact gtccaatctg gtgattctgt ttggggaatc
      841 tcaaataaat tcggtatcac aatggatcaa ttgatccaat ggaacaacat ccaaaacaat
      901 ttcatttatc caggacaaaa actgacaatc aaaggtagcc aggaaaatgg ctcttcgacg
      961 aataattccg gtaacaatac caattcttca ggaaatgctg gtacatcaaa cggaggacaa
     1021 acaactggtg cgaaatacac tgttcaatct ggtgattctg tttggaagat ctcaaatgat
     1081 cacggaatta cgatggctca attgattgaa tggaacaata tcaagaacaa tttcgtttat
     1141 ccaggacaac agctaatcgt atcaaatggc ggttcttcta atgctggagc aaatactggc
     1201 tcaaatactg ggaatacaag tggctctagt tcgtccagct caagttcttc taatacagct
     1261 tcaggaacga aatatacagt taaagccgga gactctgttt ggagcgtctc aaataaatac
     1321 ggaattacga tggctcagct aatccaatgg aacaatatcc aaaataactt tatctaccca
     1381 ggacaacaac tgactgtttc aaacagtgga tcaacaaaca gcacgtcagg taatacaggt
     1441 tcgtcaacca atacaaataa cagcagttca gttaatactg gcagcagttc gtctcaaggc
     1501 acttataccg tgaaagcagg tgaatctgtt tggagcgtgg caaataaaaa cggcatcacg
     1561 atggatcagt taatcgaatg gaataatatc aaaaacaact tcatttatcc aggacagcaa
     1621 ttgatcgtta aaggtggaac ttcggcaaat acaaatacag gttcaactac ttctgcagcg
     1681 aaaccaaata caccgaatac gtctgctacg acaagtacat catccactgg taatacgatg
     1741 tataccgtga aagcgggcga gtcagtttgg agcgtagcga acaaacatca tatcacgatg
     1801 gatcagttaa tcgaatggaa taatatcaaa aataatttca tctatccagg acagcaagtg
     1861 attgtgaaga aaggttcatc tcaatcaaca aaccaacaat cagttactgg ttcgaaaacc
     1921 tatactgtga aatctggaga atctgtttgg agtgttgctg acagtcatgg aattacaatg
     1981 gctcaactaa tcgaatggaa caatattaag aacaacttta tttatccagg tcaaacgttg
     2041 attgtagcta aataaaatag ctgttcgaag aaataacgat tgaattccac aaaaatattt
     2101 gttactataa ggctacttaa atatgacaac acttgaaaag gaagtagtaa aaagactgaa
     2161 gtttcagaga gcgcatggct ggtgagaatg tgcacggatg cttttgaact cgcctttgag
     2221 ttgcccttgc tgaatacccg agtaagcaag cggcggtgac ggccgttaca acgttcgagg
     2281 ccttttcact aaggtaaaat taggtggtac cgcgtagatt acgtcctata aaaagagcaa
     2341 tctttttata ggattttttt atttcttttg tgaagtttga gaaaacaaag caaaaggaaa
     2401 taaaaataaa cagattctgc cggttttttc gttatattta taatagaaac aagtgttttc
     2461 aatagaacag gaggacagca aatgagttac aatcataaag aaattgagaa aaaatggcaa
     2521 aaatactggg caaaaacgaa tactttcaac acccatgatg atcctgaaaa gccaaaattt
     2581 tacgcactgg atatgtttcc ctatccatcc ggtcaagggc tacatgtagg gcatcctgaa
     2641 ggttatacgg caacggatat cctttcacgt gtgaaaagaa gtcaaggata caacgtgctg
     2701 catccaatgg ggtgggatgc attcggtctg cctgctgaac aatatgcgct agatacagga
     2761 aacgatccag ctgagtttac ccaaaaaaat atcgaaacat tccgacgcca aatcaattct
     2821 ttaggattta gttatgactg gaatcgcgaa gtgaatacaa ctgatccgga gtattataaa
     2881 tggacacaat ggatctttac gaagctttat gaaaaaggtc tagcttatga agcagaagta
     2941 gcggtaaact gggtacctga attaggaaca gttatttcaa atgaagaagt gatcgatggg
     3001 aaaagtgaac gtggtggcta tgatgtagtc agaaaaccaa tgcgccaatg gatgctgaaa
     3061 atcactgctt atgctgatcg cttattggat gatctagaat tagttgattg gccagaaagc
     3121 attaaagaaa tgcaaagaaa ttggatcggt cgttcagtag gagcaaatat tgaatttaaa
     3181 gtggctggta cggataaatc ctataccgta tttacgacac gaccagatac tctattcggt
     3241 gcaacttact cggttcttgc acctgaacta gatcttgtta gagaaattac gacccctgaa
     3301 caaaaagctg ctgtagaagc ttatatcgaa gagacagcta aaaaatcaga tctaaagaga
     3361 acggatcttg caaaagaaaa aacaggagta tttactggtg cttatgcaat caatccagtt
     3421 aatggcaagg aaatcccaat ctggatcgcc gattatgtat tatcttcata cggcacagga
     3481 gccatcatgg ctgttccagc acatgatgaa cgtgactttg aattcgctca aactttcgga
     3541 ctagaaattc ttccagttat cgaaggtgga gatgttgaaa aagctgctta cacagaagat
     3601 ggtacgcaca tcaactctga atttctagac ggcatgaaca aacaagaagc aatcgataaa
     3661 atgaatgaat ggctagaaga aaatggcgta gggaaaaaag aagtcagcta tcgcttacgt
     3721 gactggctat tctcccgtca acgttactgg ggtgaaccaa tcccaatcat ccattgggag
     3781 gacggaacag tcacagctgt cccagaagaa gagctaccat tacggttgcc taagacaaaa
     3841 aacatcaagc caagcggtac tggagaatct ccattagcaa atatcgagga gtgggtcaat
     3901 gtcgttgatc ctgtaacagg taaaaaaggc cgtcgcgaaa cgaatacaat gccacaatgg
     3961 gcaggaagct catggtatta cctgcgttat atcgaccctc ataacaaaaa agaactagca
     4021 gattatgaaa agttgaagcg ttggctacca gttgacatct acattggtgg agcagaacat
     4081 gcggtacttc atttactata tgcacgattc tggcataaat tcttatacga tatcggtgtt
     4141 gttcctacaa aagaaccatt ccaaaaacta tacaatcaag gaatgatctt aggcgaaaac
     4201 aacgaaaaaa tgtcaaaatc tcgtggtaat gtagtcaatc ctgatgatgt ggtcgaaact
     4261 tacggtgcag atacactgcg catgtacgaa atgttcatgg ggccattaga tgcttcgatt
     4321 gcttggagcg aaaatggctt agaaggaagc cgtaaattct tggatcgtgt atggcgtttg
     4381 atcgttgatg agaacaataa aatgcgtgac cgtatcacta cattgaatga tggcaagctt
     4441 gataaagtgt atcatcaaac agtgaaaaaa gtgacagaag actatgaaaa tcttcatttc
     4501 aatacagcaa tctcccaatt gatggtcttt atcaatgaag cctataaagt ggacgctctg
     4561 ccttatgaat atatcgaagg atttgttcaa ctactcgcac cgattgctcc tcacatgggt
     4621 gaagaactat gggcaatcct tggaaatgat ggtggtattt cttacgcacc atggccaaca
     4681 tacgatgaag cagcacttgt tgaagacgaa gtagaagttg ttttccaagt aaacggtaaa
     4741 gttcgtgcga aaagcaatgt tccccgagac ttaggaaaag atgagctaga aaaagcagca
     4801 ttagccaatg aaatagtgca agaatatatt gaaggcaaaa cagtcagaaa agttattgcg
     4861 gtaccaaaca agttagtcaa tatcgttgct aattaattgg ctctttgtca ataaggactg
     4921 atgagttgtg caaaatcaaa tctgggccag aatgaacttc attctgtctc agattttttg
     4981 ttatttcaaa ttgattaatt gattgatcaa aaaggttttg attttcatgt tctcaaacac
     5041 gttcaatgtc tttttcttct gaatggtctt gtaagagatc tctgtaatca tcaaattttt
     5101 gtctattaat cttagtattt tttgatgtat aaactacctg aaattgagag tacataccaa
     5161 attcataaat tttttattag tgggactagt gattgtatgg attttgacgc ataatgaatg
     5221 aagtatgaga aaaggagaga caaatggatc aagaactatt tattatcgaa gtattatcag
     5281 ctaaacaaaa aattatcgag aaagcgaaaa aaagagcgaa aagaataatg ttcaagcttt
     5341 taagcttagt atttttctct ggtttgcttt ttttcggagc tgtatcgttc ctatatcgcc
     5401 atatgagtgc agtagtccgt tcagctgaag ggatgcaaaa attaaataat ttgtatcact
     5461 tttcgtgggg agttggcgga ataacaattg ttgtgcttat tgtttttctt ctacttattt
     5521 ggctccgtta ccatcaattg tcgcgtcatt tcgatcaatg gcttagtcaa agagcacaac
     5581 agtttttaca taataaacca acttctcaag atgatgacta tctttatttt gagaagactt
     5641 cgacgagtca agcttttcgg atacaaaaaa gtgattgcca gttattgctg agatcacttg
     5701 atgatttacc tatctactta ggcgaaagca aatcaaaatt cggaatgaac acattccata
     5761 tttttttact agaaaacgaa cccagatggg aaaaaagcca gtcttcgcct ggtggatacc
     5821 tgacaactaa gatgagtaca ttagtcactg gttttttcct cattctttta gctggaacaa
     5881 tctattatgg gagatacact cagccaaaaa tgtcttctca tataaatgct gcttctcaca
     5941 tgacaatgaa ctcaactaac gatacgcata tacaaaagac aaatgaaggg ttgttagctg
     6001 atcctaagca aaaggtaaat ttgacaacac aaaagataaa tgatctcaaa cttgatacag
     6061 agacgcactc tctctatatg acaacagata gtggtgctaa ttgggtattt gtgccgattg
     6121 attcgcaatg gttgcgtttt ggcgaataca ccctgacaac ggggctgatt cctgaaggtt
     6181 attggatgga caacacatat gatatttccg ccgatttctc atggtttatc tattcttctg
     6241 gggatgatgt acgtctgctg acttcaagag ataatggtaa aacatggcaa acgtctatta
     6301 tcacccaatc agttcagccg atacgttatc gaaaagcggt attttatggc aacgatggtc
     6361 tatttatctg tacatcagcc agtgggatgt ctgcagaaac tttgtacatt tacactacta
     6421 tggatggcgg aatcacttgg ataaaaagtg gaagtacaat gatcaatcag ccagtacaaa
     6481 atgtcagctt cgttacaact tctttaggtt ttgtttcgac acgtgaaagt ttgttttata
     6541 caaacaacgg aggacgctca ttcaaagaat cagttctgtc gattccggaa aactatcaga
     6601 caggggggat agatcttttt caatcaccaa atgaagttac tcaaataagc gcgaatacgt
     6661 tagagacaaa attttatttg ttgaaaaaag gaccaatcga tgcagggaaa atgtttgcct
     6721 gcctcttccg ttcaactgat aacggggaaa catggcaatt cgttcagcaa ctatcgcaag
     6781 tagaacaagg agattaaaga agagagattg tgagcctgac gttcagcacc gagtcacaag
     6841 aaacaaattc aaaaaacagc ttctcatatt tgttgaatag tgtaactcgt gattcggagt
     6901 tgacaagagc aacaagaagc gattttgaag tatagcttct cgtattttac aagatcgtgt
     6961 cttattgccg ataggttgtc tcttgaacac cgtttattcg taaaaaggga ttgtgacaag
     7021 tcttttgtca ctacttaccg aacgaaactg aaggagctat ccaaaaagta tcattagtat
     7081 tattctttgt gtttctagtg gcaccactag tacatttaaa aaagctatcg atcatggact
     7141 aatcatacat gatcgatagc tttttagata atgtttgtta cgctatctga aattgactat
     7201 tataaagact ggcataaagc gttggtgatt ggagtagttc agaatgtgtt cctttttcga
     7261 cgatatcgcc attcttcatt acaaggatca tgtctgcatt ttcaatcgtt gacaaacggt
     7321 gtgcaatcac aaaacttgtc ctatcttttg tcacagcttc cattgcttgc tgaatcaatg
     7381 cttctgttcg tgtatctacg ctagaagttg cttcatctaa aataacgaca ggaggattcg
     7441 ctagaatgac tcgagcaatt gtcaataatt gttgctgtcc ttgagataac gtgccgtttt
     7501 cgctagaaat gatcgtatca tatccgtctg gcagtgttcg aataaagtga tcacattgtg
     7561 cgatttcagc cgcttcgata atttgttctc ttgtggcatc cattttcccg taggcgatat
     7621 tatcagctac cgttccttca aacagccagg tattttgtaa aaccatccca aaagaacgac
     7681 gtagttctcc acgtgaaagc ttggtaatat caagaccgtc gaattcgatc tttcctttat
     7741 tcagttcata aaaacgcatc agcagattga ctaaagtcgt ttttcctgca ccagttggac
     7801 caacgatcgc taccgtttgt tttggcttta ctgaaaaatc gacttgattc atcaatattt
     7861 tatctgatgt gtaaccaaac tgtacatttt tgaaagctat ttttcctttc ggttgttcta
     7921 aaagtcttgc atctggaagg tctggaattt catctgtttc atctaatatc tcaaatatcc
     7981 tatcgattgc tgccattgcg gattggatcg agttgatcac ataagaagcg gtagaaattg
     8041 gttcagaaat ctgattgatg tactgaagat aagcttgtaa gaagccgatc gtcacaccac
     8101 ctgatagaac aagcatagca ccgaataccg cacttatgat gaaggcgagt tgattaatga
     8161 aacgaatggc cggatagata gcacagttca taaattgagc acgtttaaaa gcgttgtatt
     8221 gctttttatt tacttcgtgt atagcagctt ctgcatgagc ttgctgattg aatgttttga
     8281 ccactagatt accagctaag atttcttcca ttcgattatt taaaacaccc agttcatact
     8341 gattctgttc agaaatctgt ttgtttttgt tcgcaatctt agttgtaaca attgaactgc
     8401 caacgatcag caataagacg agcaaagtaa gcttgctttc gatataaaac agcatcagac
     8461 cagcagcagc tattgttacg acagaagtaa aaaattgatt gatacctgtc aataacactt
     8521 gagaaagttg attgagaccg gtagttgtcc gactaatgat gtcacctact tgatgattgt
     8581 caaaaaacga catcggtaat gttttgaatt tttttgtgat ttcttttcgc accctcaatg
     8641 tgatcttttc gcttaacgct gccattgtcc gttcctgaat aaatgaagtg atgctgctta
     8701 agactgaaca gccgatcagc aataagacag ggaacaacaa tgcttgtttg atatcactga
     8761 aagaaagctg tccaatacct gtttttctta tttctgataa gagatcatcg atcccgattc
     8821 ccatgatcaa gggcatggct acgactaata ggttcccgaa taaactgcat agaaacaatc
     8881 cgtaaaaata tttatgttca ggtttgatca ttttccaaaa acgtatcaaa gcacgcgtgt
     8941 tcaacgtttt tttcttttcc atcgtatccc ttctttctat attccttgtg attttgcaaa
     9001 ctcctgataa gtttggcaat tagacagtag ttcttcatga gtaccttgtc caacgatttt
     9061 tccttcatct aaaacaatga tgttatctgc ttgttggatt gtactgaggc gttgagcaac
     9121 aatcacgaga gttttctccg acatgtgttt atgaagggct gctcttaatg ctgcatctgt
     9181 tttatagtct aaagcggaga aactatcatc gaatacatag atctctgctg gtttgatcaa
     9241 cgctcgagca atacatatcc gctgtttctg gccccctgaa taattagaac cgccttgagc
     9301 aacaaagctg tccaatccgt caggaagggt gtccactagt tcttctaatt gagcgatctt
     9361 gatcgcttta gacaactctt ctgttgaggc atattcattt cccatctgca agttgcttaa
     9421 aattgtcccg ctgaataaga aagccttctg agggacataa ctgatgtggg agcggatctg
     9481 ttcttgagtc atttgagtaa gaggaatacc accaagggtt acttttccta tcgtagggtc
     9541 actaagtctc aagagaagtt tcgccaaact actttttcca gatccggtcc cgccaacgat
     9601 cgctgttgtt tttcctttag gtattgtgaa atcaatatct tctagaactg gatcagagat
     9661 ttcagtatat ttaaatgtga catgttcaaa agcagctgca tttgtctgat caaaggttgg
     9721 ttcagctact gttttgatcg tctcttcctc aatttcgatc tctgcttgca gtatttcttc
     9781 tatccgtcgt aatgaggcaa acgatttagg gataatgacc aataccatag ctgcgatcat
     9841 caagtaagca agtgtcagca tagagtattc gactacagca gtcacgacac cgatttgtaa
     9901 tgctccttct gctacaagat ttccgccaaa ccacaaaata gaagcataaa cgattcccat
     9961 gagcaaaaaa gcaattggag tgacccaagc aaatattcgg ttcactttga tcatttgatt
    10021 agcatagtca gcaaagctct tattcgttcg cttttcttca tatgattgat tatcaaaggc
    10081 acggatcata ttaatcccag taaagaattc tctcaatgtg atggtgatcc gatccatttt
    10141 tggctggata ctcaaagata aaggtgcccc tcgtttcatc agtcgataaa caatccatac
    10201 ataaaaaaca acagagatca atgtaaccca tgttagtaat agagaataac gaaacatcat
    10261 gagaatggtg aaaacgcaaa taaccggtgc tggtaagatc atttggcaaa ataaaacaat
    10321 catttgctgg acattatcga catcattcgt cattcttgtc aaaagagatc cagaactaaa
    10381 acgactgata tccttcattg acagctgttg gaatttacgg aaatatttct cccgcgtttg
    10441 gaatccaaac ctagcggaag ccaatgctga aaagtaacta gaggcaactg cagcgattgt
    10501 tcctagaaac gctacaagca gcatttcaac tccaattttt tttataactg aggcttcgcc
    10561 acttgctata cctgtgtcga tcagctttgc tactaatttt ggtacaccta atgtaccggc
    10621 gatttctaaa cagatcatca caaaggtaag gagagcgagt cctttgtttt tctgtaagaa
    10681 tgtttttaag atagacatat tattcctcct tttttcctga tacttttcga ttctatacct
    10741 tcaagtaact tgaaggtcaa tagaaaaaag aataaactga aaaagaggtg agaaaaaatg
    10801 aaaaaaatgt atacgattgg ccaagtatcg gaactatttg atttgccaaa atcaacgatc
    10861 cgctattggg atgaacaagg attaatccgt tcaagtagac aagaagagaa tgattatcga
    10921 ttgtttgata tcgatgatat ttttatgctt tatgatatcg ttttttatag aaaattagat
    10981 ataccgatca aacagatgaa gaatttatat ggaaaaacat tgacggagtt atatgagaca
    11041 cttgatgaaa cagaaagaag aatccataaa gaactggtgg tgatgaaaca aaaacaaaaa
    11101 gagataaggg agcaaaaaaa acagttgaaa ttgatgatcg atacaaacga ggaagaattt
    11161 cctgtagaag agatcccttt tgactgtatg atctcaacgg agtttgaaga tattgtggaa
    11221 atcaaaaaat ttctacctaa ctattcaagc tttgggatga tgagtatgcc agcttccagt
    11281 tcacagacga tgtatggatt ttttatcgac cctagtgaag tacatttatt tactcaagac
    11341 gtgatttggg agaaaaaaga tacggctgtt tatcgtcgat tcttattgaa atccgagatg
    11401 aaccatgcgg aacgtaataa tattttagaa atcagagaaa gaatgtatga aaaaggatgg
    11461 aaaacaggag aagtgatcgg ccagtatcta ttgacgaata cggatgaaaa caatatcaga
    11521 actgaatatt atcatgcatg gatagaaatg aaaaaatagg aaatatgtgt ttaagataac
    11581 tcgaaaatga tggttgtttt agttataaag aaaaattttc tggttcgatt acgggtttaa
    11641 tgtgggggat tattagtcgt ggatagatag aattttagtt ttttagagga aatatgtctt
    11701 taaaaatgga atggttgcaa ttcttagcga tatatacgat gtaattgtcc tcttaaaaaa
    11761 gtatggaaaa ataattgtat tgggtgttat gtataattca tggtgaactg agactaatga
    11821 gaagaaaaat tcatattgtt atgagtgaga ttgtatcaga tgagaatgat aaattaaata
    11881 ggtgcataaa agcattacac ttaaaataca ttgttgctta ca
//
LOCUS       DAZKNH010000082        11603 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000082 DAZKNH010000000
VERSION     DAZKNH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.082"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8MG_002063"
     CDS             6..305
                     /locus_tag="ACG8MG_002063"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0376088.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8MG_002064"
     CDS             378..1739
                     /locus_tag="ACG8MG_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFQ0376089.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8MG_002065"
     CDS             1761..2021
                     /locus_tag="ACG8MG_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376090.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8MG_002066"
     CDS             2035..2787
                     /locus_tag="ACG8MG_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFQ0376091.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8MG_002067"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8MG_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0376092.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8MG_002068"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8MG_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFQ0376093.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8MG_002069"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8MG_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0376094.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8MG_002070"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8MG_002070"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0376095.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8MG_002071"
     CDS             7161..8558
                     /locus_tag="ACG8MG_002071"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0376096.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8MG_002072"
     CDS             8639..9079
                     /locus_tag="ACG8MG_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0376097.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8MG_002073"
     CDS             9227..9553
                     /locus_tag="ACG8MG_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0376098.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8MG_002074"
     CDS             9554..10303
                     /locus_tag="ACG8MG_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFQ0376099.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG8MG_002075"
     CDS             complement(10383..10838)
                     /locus_tag="ACG8MG_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0376100.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACG8MG_002076"
     CDS             complement(11265..11540)
                     /locus_tag="ACG8MG_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376101.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3662 a         2014 c         2357 g         3570 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa aca
//
LOCUS       DAZKNH010000083        11199 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000083 DAZKNH010000000
VERSION     DAZKNH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.083"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..898)
                     /gene="ftsX"
                     /locus_tag="ACG8MG_002077"
     CDS             complement(14..898)
                     /gene="ftsX"
                     /locus_tag="ACG8MG_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFQ0376102.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(891..1577)
                     /gene="ftsE"
                     /locus_tag="ACG8MG_002078"
     CDS             complement(891..1577)
                     /gene="ftsE"
                     /locus_tag="ACG8MG_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFQ0376103.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1682..2783)
                     /gene="prfB"
                     /locus_tag="ACG8MG_002079"
     CDS             complement(join(1682..2710,2712..2783))
                     /gene="prfB"
                     /locus_tag="ACG8MG_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFQ0376104.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2909..5443)
                     /gene="secA"
                     /locus_tag="ACG8MG_002080"
     CDS             complement(2909..5443)
                     /gene="secA"
                     /locus_tag="ACG8MG_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFQ0376105.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5665..6216)
                     /gene="raiA"
                     /locus_tag="ACG8MG_002081"
     CDS             complement(5665..6216)
                     /gene="raiA"
                     /locus_tag="ACG8MG_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFQ0376106.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6344..7063)
                     /locus_tag="ACG8MG_002082"
     CDS             complement(6344..7063)
                     /locus_tag="ACG8MG_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFQ0376107.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7060..8373)
                     /locus_tag="ACG8MG_002083"
     CDS             complement(7060..8373)
                     /locus_tag="ACG8MG_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0376108.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8454..8774)
                     /locus_tag="ACG8MG_002084"
     CDS             complement(8454..8774)
                     /locus_tag="ACG8MG_002084"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0376109.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8882..9169)
                     /locus_tag="ACG8MG_002085"
     CDS             complement(8882..9169)
                     /locus_tag="ACG8MG_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376110.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9296..9940
                     /locus_tag="ACG8MG_002086"
     CDS             9296..9940
                     /locus_tag="ACG8MG_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFQ0376111.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9999..10706)
                     /locus_tag="ACG8MG_002087"
     CDS             complement(9999..10706)
                     /locus_tag="ACG8MG_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0376112.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10926..11096)
                     /locus_tag="ACG8MG_002088"
     CDS             complement(10926..11096)
                     /locus_tag="ACG8MG_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376113.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3053 a         2430 c         1957 g         3759 t
ORIGIN      
        1 ttatattgtg gtattagaat ttcaaaaaac gtctcataga aatgatcgag ccaagtgaac
       61 caatgatcac accaatgccg accattaaaa gattgatttt ccaaacgata ttttgtggac
      121 gaatcaatga ataatgggaa cgtaataatg atggattgat caattggaaa aaccatgcat
      181 aaccaaatgt gatgatcaaa atcggaagaa tcgcaccgat cagaccgatc cagccacctt
      241 caaggaaaaa cggccagcga atatatccat tttttgcacc gactaaacgc atgatttgaa
      301 tttcacgctg tctagacaaa atcgtgatcc gaatcgtgtt cgaaatgaga aacatcgcta
      361 cgaacagcag caatgccgct gcaactaatc cccatgtccg aactgcacca gcgatttgga
      421 aaatccgatc tgaggaagct ccaccgtaat cagaacgaga aacattcttc aaatctgctg
      481 cttcttctgc tacttgtttt gtataagaag gatcagtagc acttactaca tacacatcat
      541 ataacgggtt attgtcacct tcaaacaatt tccaggaatc acccatttcg ttttggattt
      601 ttttcaattc ctgttctttg cttgaaaatt caactttttt aacatgatct aagtcattca
      661 attctttttc tagctttttc atctcttcgt cttttgtacc gatatctacg aatacagata
      721 catcaacgtt tccttctata tcctgagcta atttagttgc attcatgata actgccatga
      781 atacaccgac taaagtcaac gtgatcgtta ccgcactgac tgaagcaact gtcatccagc
      841 cgttacgttt caaactcttg atactttcta ataaatggcg gaaaaaggtt ctaatcatcg
      901 tatccatatt ccccttccgc ttgatctcga ataatgcggc cgttttcgat agcaattact
      961 cggtgccgaa tcgtatttac gatcgtactg ttatgtgtag ccatcacgac agtagttcct
     1021 tgggcattga tccgatctaa taatttcatg atttcccatg agttttctgg atccagattt
     1081 cctgtcggtt catccgcgat aagtacttta ggcgtattca cgattgcccg agcaatagag
     1141 acacgttgct gttccccacc ggataattcg ctaggaaaga ctcgaacttt gtgtttcaaa
     1201 ccaacaaggt ccagcacttc cattactcgt tttttgatat ctcgaggttt tctgccgata
     1261 acttgcatag cgtacgcaac attttcatat actgtttttc ttggtaataa tttatagtct
     1321 tggaacacca cgccgatttc tctgcgtaga taaggaactt cgcggttttt gattgctatt
     1381 agatcatgac ctgcgacgtt caaagttcct tttgaagctt tttcttctcg atacatcaat
     1441 ttgatgaatg tagatttacc ggctccagac ggaccaacca catagacaaa ctctccttgt
     1501 tcgatgacaa tcgagagatt gcggatagcg gtcgtaccat ttgagtactt cttcattaca
     1561 tccttcattt caatcatggc gttctctcca tatctttttc agtctttagc tattatagca
     1621 tggcaatgtt tcaacatgct tgcaaaatct tacaatttta tttcaattta acaatgagtt
     1681 cttattgtag atgcattttt aaatagccgt caataaataa atcaaggtca ccgtccataa
     1741 ctgcttggac atttcctgtc tctacgtttg tccgatggtc ttttaccata gaatagggat
     1801 ggaaaacata agaacggatc tgtgaacccc agccgatttc tttttgttct cctcgaagtg
     1861 cagctgcttc ttgttctttc ttttccactt ctaattgata aagcttagct tttaacatcc
     1921 ccatcgcttg ctcacggttt ttcaattgtg agcgttgtgc ctgactagct actactgttc
     1981 ctgtaggcaa gtgagtgatc cgtacagcag attctgtttt attgatgtgc tgaccacctg
     2041 caccacttgc tcggtacgta tcgattttca aatcatctgg attgatttct atatcgatcg
     2101 tttcatcaag ctctggcatc acatctaccg aacaaaaaga agtatgacgt cgtttcgctg
     2161 agtcaaaagg cgaaatccgt acaaggcgat gaacaccttt ttctgatttt agataaccaa
     2221 aagcgttatg tcctttaatc agcagggtca cactcttgat ccctgcttca tcacctgctt
     2281 gataatctag tgtttctact tgatagccat gctgctctgc ccaacgtgta tacatacgta
     2341 aaagcatgct gccccagtct tgggactccg ttccgccagc ccctggatgc aattcgacga
     2401 tcgcattgtt tcgatcataa ggtccgtcta acagcataga taattcatac gtcttcattt
     2461 tttcatctaa tgcattgata cgtgtttctg cttcttcttg cattgctgca tcaggttctt
     2521 cttgcagcat ctccaccatc aattcgattt cttctagttc ttcggcaaga gaatggaatt
     2581 ggtcataggt ttctttatta gcgttatttt cgctgatcac tttttgtgcg gcttcgctgt
     2641 catcccaaaa accaggttca gccatttttg cttctgcttc cgcgatgtct tcttccagct
     2701 gatctaagtc aaagagacct cctgaaactt gtgacctttt cctgcatttc tgtcaggaga
     2761 tttcgaattt cggatatttc catgtttttt ccttctttca ctatactttt ttcattatat
     2821 ctagtctaac agaatcagca aataacagaa agaaggtgag acgaattctt aaaaaaagaa
     2881 tttgtctcac caatcctgct gtcagaaatt agtttgcatt ttttccatga cagttcttat
     2941 attttttccc gcttccacaa ggacaaggat catttcttcc gaccttgttc acatggattg
     3001 gctgtttttt cgcgctagta ttactatttt ctttggcatc tccgtcttct gagtgagaag
     3061 ctgttccttg agaaacctgt tcacgctgta cgttttgacg gatctcagct ttcataaaga
     3121 ttcgagtgac ttcatactcg atggcgccta ccatatcttc aaacatctta tagccttctg
     3181 tttgatattc gactaatgga ttgttctgtc cataagcacg taagccgatt gactgacgta
     3241 attgatccat tgcatcaata tggtctgtcc atttagagtc cacaacacgc aagatcacga
     3301 ctttttcaaa ttccaacagt tgctctggac catttagttg agccgctttg gtattaaata
     3361 cttcttgtgc tcgttcgttt agatactctt tgatctcctg agatgttttt ccttcgatat
     3421 ctgcaagagt aatactatct tcatggacaa gagtactgcc ggcaaaatcg atgatacttt
     3481 ccaaattcca attttctttc tctaattgtg tatgcgcatc tactacacga ctgattgtcc
     3541 gtttgaccat gttcattagt ggcttggata agcttttttc ttccatgatt acttcttgtc
     3601 gctgtttata gattacttca cgttgttcac gcatcacatc atcgtattgt aagacgtttt
     3661 tacgtgtgtc atagttattt ccttctacac gtttttgtgc agattctact tgtttcgtca
     3721 gcatcttgct ttggataaca gcatcttcct caccgatctt catccgatct aagaaagctt
     3781 tgattcgttc tgaaccgaat cgtttcatca aatcatcttc caatgacaag tagaattgtg
     3841 acataccagg gtctccttga cgtcctgcac gtcctcgaag ctggttatcg atacgacgag
     3901 attcatgtcg ttctgtcccg ataactgcta gaccaccaag ttctcttacc ccaagtccta
     3961 acttgatatc tgttccccga ccagccatgt tggtagcaat cgtcacagca cctttttgac
     4021 cagcattcat gatgatctct gcttctttaa agtgattttt cgcattcagc acttcatgag
     4081 gaatacgttc ttgattcagc atatctgata ataactcaga tgtttcaacc gcaacagttc
     4141 cgactaacac aggttgacct ttgcggtagc gatctttgat atcttgcacg acagcgtgga
     4201 acttgctttg taatgttggg taaagtaaat ctggacgatc atcacggata accggacgat
     4261 ttgtcggaat ctggtatact tggatgttat aaatttctct gaattcttct tcttctgttt
     4321 tagctgtacc agtcattccc gcaagtttct tgtacatacg gaaatagttc tggaaagtga
     4381 tcgtcgccat ggttttcgtt tcgtcttcga tctctacgcc ttctttagct tcgattgctt
     4441 ggtgaagtcc atctgaataa cggcgtccat ccatgatccg accagtgaac tggtcaacaa
     4501 tcagtacttt tccgtcttgg acaacataat cgatatctaa aagcatgata tagttggctc
     4561 tcaatgcttg atccaagtga tgggtcaatg cagtattttc aatgtcgtat aagttatcca
     4621 aaccgaatgt ttcttctgct ttttcgatcc ctgcttcggt taaactgatt gtttttgatt
     4681 gtacatcgat tttataatct tcttcttctt tcaaacgttt cacgaagttg tctgctcttg
     4741 tatataaagc tgtggatttt tctgcttgcc ctgaaatgat cagcggcgtt ctcgcttcat
     4801 caatcaagat tgagtcaact tcatcgacaa tggcataatt cagcggacgt tgtaccattt
     4861 gatggcggta aacgaccata ttgtctctca agtagtcaaa tcctaattca ttattcgtac
     4921 tataagtgat atcacaatta taagcctcgc gtttttcatc cgaagatttt gagttgatat
     4981 tcaaccccac tgttaatcct aagaaattat aaagttcgcc catttccgtc gagtcacggg
     5041 ttgctaaata ttcattgacg gtaactacgt gtaccccttc gccagtcaga gcatttagat
     5101 atactggcat cgttgcggtc aaggttttac cttcaccagt acgcatttca gggatatttc
     5161 catcgtgaag cacgattcca cccatcaatt gtacgtggta tggatataac cccaatacac
     5221 gttttgctgc ttctcggacg actgcaaaag cttccggaag taattgatct aatgtttctc
     5281 ctttttggta tctgcccttg aattcagcag tttttgcctg taattgttca tcagttaatg
     5341 ctgccatttg gtcagcgtat gcttcaactt tttttgcaat gctatctaaa cgttttagtt
     5401 ctttcttatc gttttcgata agtgaacgaa tcaaattggc catgcgttgt gtaatctcct
     5461 tttccttctg tgtattatcc agactatgta gactttactt ttcattttat cattagttat
     5521 agataattga aatatcttaa gcgtattaaa gtaagaactt cataatttat tcatatttca
     5581 tctaaaagag gaggaaactc aaaaaaactt gcggcagctg tcagtagctg ctgcaagttt
     5641 tcgttgttat ttaagtaagg ctgatcagtc tgtttcaatc agtccgtatt ttccatcttt
     5701 acggcggtaa acgatacttg taccatttgt ttctgcatct tcaaaaataa agaagttatg
     5761 tcccagcatg ttcatttgta agactgcttc ttcactatcc attggtttca atgaaaggcg
     5821 tttcgtacgt acgatatcta attcagaatc attttcgttt tcgtcctctt cattgaataa
     5881 agcagcagca tctgcagtat tcatgcccgt ttcacgggat ttacgattaa tttttgtttt
     5941 gaatttgcgg atttggcgtt ccaatttatc aacaaccaaa tcgatacttg cgtataaatc
     6001 tggagaagtt tcttcggcac gtaatacaag ataaggtaat gggatcgtta cttccacttt
     6061 tgctgttttt tctgtataaa cttttaagtt cacatgcgcc gttgcatcgg gtgcgtcact
     6121 gaaatagcgt tctagtttgc ctactttttt ctcaacgtag tcgcgaatcg cttcagttac
     6181 ttcgatattt tcgccgcgta cattatatct aaacataaaa attacccctt tcgtctacca
     6241 caatcaagaa ggagaaggac cactctttcc ctcttcttaa ttctattata tcgaactcta
     6301 ggttatttgc aaattaaatc gctatcaaat ttccacatct aattcatctc gccaaagaaa
     6361 tggttcggat gattttcggc tgtgcgacct gcaaacaatc tgctgcgtgg aaaagagtct
     6421 gtcctgttgt atagacatca tcaataatca atattttttt attttgtatc ttagaaaagg
     6481 tgactgcaag ttcaaaaggt tgaggcatct ccaatctttc ctttcgcgtg ttttttgctt
     6541 gagccagcag atgcttctct cttttgagta gcatctgaaa aggaattctt gctgctttca
     6601 acatctcgct tacttgattg aatcctcttt ctgttcgcct tttttcagat aaaggtattg
     6661 gacaatagat gtatgtcggg tattcttttt ttatctgcgt ccatttatat gcaaaggtgc
     6721 ctgctagtcg gatatctccc ataaacttgt attgattgag ccaggccttc atattttcat
     6781 cgtacacaaa aaaggcacgg tgctgaaaat cataggtcgg atatttcttc ttccattctt
     6841 gacagtcact gcagtaaacg ggaatagact ttactttctt tttcgtactc tctagattga
     6901 aatggattgg tttttgacag ccagaacagc ctcttgtttc caataaatga aattgtgcgc
     6961 gacaaactga acaagtctga ggtaatctgt atttcgaaac accaataatc tcgctgaatg
     7021 ttaaattttg gaagatatct ttactgcagc aactgcacct cacagcatct tcttcccttc
     7081 ttcattcatt tctttgattt cctgacaagc ttttcgaata gctgcagttt tttcttcata
     7141 gaaaaagtat acttgactgt tcgtataagg ccctttacga tcggctcttc ctgcgatttg
     7201 taccaaagaa gatttagtaa aaaccggatg atccgctcct aaaacgataa cggatattct
     7261 ttcaaatgtg actccacgtt caaggatcat cgtggttaac agtatatcat attttgcttc
     7321 tcgcattttc tggacttttt ccgcccgctg attgtctctt gaagaaacat ctgtgatctc
     7381 atatctcgga agagcctctt gaagtgtggc tttcaaacga tgcatcatcg tgatacttgg
     7441 acaaaataaa aggatatggt tttctttaat taattgatga atgatccgca ccaaagattt
     7501 tatcttcttc ttgttcttgc atttttgtcg ccaattattc atgaaaacca agacgggttc
     7561 aggaagcaaa cgccgatgaa agcgtaaagc aagtttgtgg atgtcaaacg tctgcttgat
     7621 ttcctttagt aactttttat caggtgtggc acttaagtag atgaatcgtc cgtctggttt
     7681 aatcgctgtt tgcacagcat gagctaatcc tgcatccccg ttataaggaa acgcatcgat
     7741 ctcatcgatc actattaaat caaaggaccg ataaaaacgg tatagctgat gaatcgtaca
     7801 gataacaaaa gaagtaaagg tataagccgt tgctgtattc ccatggagca acgtgatttt
     7861 ttcttctgga aaaacataac gatagcgtaa aaacaattca ttacaaacat cgactcgcgg
     7921 tgtacaaatc gctactcttc ctccagcttt taaagtttcc actaaaatag gataaagcat
     7981 ctctgttttc cctgcacctg ttacagccca aataagatga tgtctttttt ctgaatggca
     8041 aagtattctc tttgagattt ccgtttgttt ttcagtaagc tgtcctgtcc aaaaaaagac
     8101 gacttctcga ggttttgctt caggctgatc aaacaaatat aaatcttgtt gagtgtcgca
     8161 tcgccctaaa tatagacaat gtgtacaaaa ataaccgtaa tctgcaagct gtccatcaat
     8221 gacggacgtc ctcattccac agcgatgaca aagaatccag ttgcccaaac gttccactgc
     8281 tgcgatcttc tcataatttg gtaaatttac ctcctcacag tctattgctt caatatctct
     8341 cgtcagaagc cttctacccc aaaaatgatt catattcttc gccctaaaaa aatatacgaa
     8401 aaaacaagcg atctattttt tggatcgctt gttcgatagt tatactgttc tatttattca
     8461 cctatgagcg tgattgcttg atctaacact ttttcgccat tcatcatacc ataatcagcc
     8521 atattgatga catctaaagg aatgcctttt ggttccagac gtttctcaaa atcagctttc
     8581 ataaagcgaa cttgtgggcc taataacaac acatccacgt ctttatttgc aaggttgtta
     8641 tctgcgtcag aagcggacac agcgaaaata tccgagtcga ttccttttgc ttcagcagct
     8701 ttttgcattt ttgttactag taaacttgtg ctcataccag ccgaacatac aagcatgatt
     8761 gtttttttag ccatactttt ctctcctttt taaaacaaac tatgcaaacg ttttcttcgc
     8821 gtctgttact ctattatata aaaagaaaga aatttgtcaa cgttttctaa acgcggttta
     8881 tttaatcaaa tagccaacta gatttcacta atttattgaa cacgacatat tctttgtcca
     8941 aattgatgac aactacttca tagcctgcat ttatccagcc ataggcacct ttttcagggt
     9001 gtttataatc atttgcccac caatctgtac tttcccttgc tgttttggga agtccgttac
     9061 tgggaaacag cagttcgtca aattgggtaa aggtcaacgt tacttgagag ccgccattgt
     9121 tcttcaaata atgcgtaatt gcatcgtact ttttcattcg ttcctgcatc cccagatcca
     9181 cctgcctttg tctttattat cgcatactac agcaattgcg caaccgttac agtgttgaaa
     9241 caacctagaa ctttatttat actgaataaa gaaaatgaaa aaaagaggtg acaagatgat
     9301 cgagcgctat cgtacaataa aagaaaatgg acaaagtgag attgagatca aaaaatcacg
     9361 ctttatctgt tctcttagac gaatagaatc agaggaccaa gcaaaaagtt tcattcaaga
     9421 aataaaaaaa gaacattgga aagcgaacca taactgcagc gccttcatca ttggagaaaa
     9481 aaatgaaatc cagcgaagca gcgatgatgg agaaccaagt ggaacggcgg gcgtaccgat
     9541 gttggaagtc ctcaaaaaaa atcaactgat caatgtagct gcagtagtca ctcgatattt
     9601 tggaggaacg aaactcggca caggcggatt aatacgagca tacactcatg cggtctctca
     9661 agcattgtct gacattggaa tcgtagaagg taccttgcaa caggagatct ctgtgatgat
     9721 cacgtatccg caactaggaa aattacagaa ttatcttgaa ctccaacatt tttttataaa
     9781 agaaatccaa tatactgatc aagttagcgt catctgtgct gtagatgaaa aaagtgctga
     9841 ccactttaaa gaatccatca ttgagctgct gaacggacaa gtccaatttg aagagggttc
     9901 tgtttcttat cacgagcaac tgataactga tcatatataa aaacaggtga ttctgattct
     9961 cttttatttc taataaggaa tcagggatca cctgtttttt atttttccaa ataaaactca
    10021 aaacgatttc ctacgtattg cgtacgcaca tactcaaacg gcgtaccgtc ttcaaaataa
    10081 gaaatctggc gcaaacgaag aactgcatct cctcgtttta cctctaaata gtcagcaatt
    10141 ttctcagaag ccaacacagc agtaatcgtt tgattcgctg gaccgatctt tttcccgcct
    10201 ttttcttcta gcgtgcgata caacgattca gtaatctctg ctttgctgta gccatcgatc
    10261 agcttttgtg gaacactggc aacttcaaaa caaataggga catcatctgc gaaacgaata
    10321 cgttccattc gcaagatcgt gtcatcttta ctcaagttta gtttttccat ttcacttgag
    10381 cttggcgaag tcaagaaata agagattgtc cgactagacg gtactcgatt ttgtgattcc
    10441 ataatttctg taaaacttgt tgcaccagac attttttctt gtactttttc tcgcgcaaca
    10501 tacgtacccg aaccgatctt tctttctaag attccttcat ctgccaagtt ttgaattgct
    10561 tgtctcaacg tcatacggct gacattgaat ttcactgcta attctctttc tgaaggaaga
    10621 cggtctccga ctttccatat accctgttcg atttcatttt ttaattggtc atgaatctgt
    10681 atatatacag gtaattgatt cgacataacg tacacccgtc ctttcgtttc ttctattata
    10741 actggtatat acctgattca caactgaaaa agataaaggt ttctatccat caacagagga
    10801 tttagacaca catcgttctc cctactccta ttgtcagaaa caatttttta gatatgttca
    10861 acctagaaaa taacaaaaga ccctaaaatc gatgaaaatg ccgacttatg gatcttttgt
    10921 tattattagc ggtttttagc catccatttt tttgtatgat tcgttgggcg ttttgatttt
    10981 acctggaccg cttttccgcc ttttttcgga atattcactt caatattgta tttttctgat
    11041 ttgaccaaat catataactt ttcagctgct tcgtctactt tttctttcca tttcatgtgg
    11101 atccctccaa ttcttgtagt taacttaact ctatcttaat tcagcagaat cttcaaggaa
    11161 caaggtagca aagcaaaaaa aagagctctc gaactaacg
//
LOCUS       DAZKNH010000084        11159 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000084 DAZKNH010000000
VERSION     DAZKNH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.084"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..1380
                     /gene="lysA"
                     /locus_tag="ACG8MG_002089"
     CDS             121..1380
                     /gene="lysA"
                     /locus_tag="ACG8MG_002089"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFQ0376114.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1367..2167
                     /gene="dapF"
                     /locus_tag="ACG8MG_002090"
     CDS             1367..2167
                     /gene="dapF"
                     /locus_tag="ACG8MG_002090"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFQ0376115.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2170..3051
                     /gene="dapA"
                     /locus_tag="ACG8MG_002091"
     CDS             2170..3051
                     /gene="dapA"
                     /locus_tag="ACG8MG_002091"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFQ0376116.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3048..3764
                     /gene="dapB"
                     /locus_tag="ACG8MG_002092"
     CDS             3048..3764
                     /gene="dapB"
                     /locus_tag="ACG8MG_002092"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFQ0376117.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3770..4465
                     /gene="dapD"
                     /locus_tag="ACG8MG_002093"
     CDS             3770..4465
                     /gene="dapD"
                     /locus_tag="ACG8MG_002093"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFQ0376118.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4481..5482
                     /locus_tag="ACG8MG_002094"
     CDS             4481..5482
                     /locus_tag="ACG8MG_002094"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFQ0376119.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5479..6840
                     /locus_tag="ACG8MG_002095"
     CDS             5479..6840
                     /locus_tag="ACG8MG_002095"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFQ0376120.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6800..7951
                     /locus_tag="ACG8MG_002096"
     CDS             6800..7951
                     /locus_tag="ACG8MG_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0376121.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7926..9095
                     /locus_tag="ACG8MG_002097"
     CDS             7926..9095
                     /locus_tag="ACG8MG_002097"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0376122.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9270..10649
                     /locus_tag="ACG8MG_002098"
     CDS             9270..10649
                     /locus_tag="ACG8MG_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0376123.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10930..11159
                     /locus_tag="ACG8MG_002099"
                     /pseudo
     CDS             10930..11159
                     /locus_tag="ACG8MG_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3717 a         1949 c         2497 g         2996 t
ORIGIN      
        1 atttcgaaat atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca
       61 aatctttatc aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa
      121 atgacagaat tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca
      181 cctctgtatg tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc
      241 tttgtatcga aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt
      301 gagatgctgc atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa
      361 atctataccg ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac
      421 aagacgccag aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat
      481 aatttgatgg aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt
      541 atcatgcttc ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg
      601 catattgatt caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag
      661 ttgataaaag aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa
      721 atttttgatg ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat
      781 tttgaccaac ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat
      841 gatcagccga tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct
      901 ttggaaaaac gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt
      961 ggagaagctg gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa
     1021 tattactttg tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa
     1081 tacgattgtg atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca
     1141 ggaaaaatgt gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa
     1201 tcgggtgatc ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac
     1261 tacaatcgag cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt
     1321 gttcgcagac aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa
     1381 atataatggc tgcggaaatg attttatctt attagattac gcagaagaca cagattatag
     1441 tcaattagcc gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt
     1501 aaaaatcgat ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg
     1561 cggaaatggc attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca
     1621 ggaattcaac gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc
     1681 tttttattgc agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa
     1741 tcaaacttat ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac
     1801 tagtttattt ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt
     1861 ctggagaaaa agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt
     1921 caactttgta gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt
     1981 aggttggaca cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac
     2041 tgggaaaatt tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc
     2101 tggtgaggat gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga
     2161 ggaatgaaga tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga
     2221 tcagtagatt atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat
     2281 ggattattag ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag
     2341 cagatccttc aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct
     2401 ggaacgaata atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca
     2461 gacgcacttt tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct
     2521 cattttacag caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct
     2581 agaacaggca tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc
     2641 attggtttga aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt
     2701 ggagaagaat tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta
     2761 ggaggaagcg gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta
     2821 gtaaaagata cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt
     2881 gaactgatcc atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca
     2941 ttattagact taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa
     3001 aagaaacaat tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac
     3061 tgattggcag tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata
     3121 cggtccttct tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc
     3181 cagaactgct tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct
     3241 tatcatatca acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag
     3301 aaataaaaga acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca
     3361 tcatggtgat gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg
     3421 aactgattga aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc
     3481 tgcttcaaac aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa
     3541 aaagagaaga aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac
     3601 atgatgtatt gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta
     3661 atcgaatctt tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag
     3721 gttattacaa attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca
     3781 agaaatcatc cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa
     3841 tgtaaccgag ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt
     3901 tgtttttggt gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat
     3961 tgaaatcgaa atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa
     4021 aattaatgct cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa
     4081 tgccgtgatc atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat
     4141 gattgatatg ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg
     4201 tgcaggagct gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga
     4261 agacggcgta ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga
     4321 tgcagtcgtg gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg
     4381 tggaatacct gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt
     4441 gattgctgcg ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg
     4501 tattgtgggg gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga
     4561 ttttccagta ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt
     4621 attcaagaaa aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga
     4681 tttagcgcta ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag
     4741 tcagggacta tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt
     4801 aatcgttcca gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa
     4861 ttgttcaacc attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca
     4921 gcgagtcaat tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga
     4981 tcttcatcgt acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac
     5041 ggtgattccg gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat
     5101 gataaatgag acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt
     5161 acgtgtgcca atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt
     5221 tacgacggaa gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga
     5281 tctagcagca cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt
     5341 tgggaggatc agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga
     5401 taacatacgc aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa
     5461 aatagcggag gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg
     5521 atgtctcatg aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc
     5581 caagtgatcg ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac
     5641 ttactttatt tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa
     5701 aggatttgtg atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa
     5761 gatgaactag cacgcataca gaaacaactg gaagacgaaa agataactga agattattta
     5821 gtaagtagag gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt
     5881 attgacgcaa aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca
     5941 gcacaggaaa tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac
     6001 ggggcgaacc ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc
     6061 gcgatactgg cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga
     6121 atcatgatgg ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt
     6181 agagaactga gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc
     6241 ccagttcaag aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa
     6301 gggacaaaaa tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga
     6361 cgcaaggatt ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt
     6421 atctggaaag caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca
     6481 gggatcgaca atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc
     6541 atcacgaaag aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta
     6601 gctttgatct ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg
     6661 ttatcgatat tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag
     6721 ttgaatctga tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat
     6781 gaagggatgg tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga
     6841 aatcgggttt catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga
     6901 ggccgttatc catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga
     6961 agaaacgatt gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct
     7021 ctcttttgct tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc
     7081 gatgctttta gggttgactg actttataac taaacacatt gaggatctta tttataatgt
     7141 agtactgatc tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc
     7201 aggattgttg gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact
     7261 agctaaggga gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga
     7321 cattatcatt aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct
     7381 ggaggcagct gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga
     7441 gtggaatcat ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc
     7501 tggagaagtt attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt
     7561 gaagagggga ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg
     7621 ctataatgac ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga
     7681 ttcaaacaaa ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata
     7741 tacgcaacaa tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca
     7801 tgatccgacg ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac
     7861 gattcttttt acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt
     7921 aaataatgat gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa
     7981 aaatacgaca attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg
     8041 gagaaccgga ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag
     8101 aagacttttc tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta
     8161 tatttcagca acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag
     8221 ttggtgcaac ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg
     8281 ttcttattcc agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg
     8341 aattgatccc gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac
     8401 gagcatttga acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc
     8461 ctactgggac gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat
     8521 atccggtatt tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata
     8581 tgtccatagc tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac
     8641 atgcgatgac tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac
     8701 taattaaagt ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg
     8761 ctgaagcgct aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac
     8821 gaagagacta tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg
     8881 gtgcatttta cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt
     8941 gtgtagaatt agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag
     9001 aaggagaagg ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct
     9061 gcaaacggat caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa
     9121 tcgttaattt tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg
     9181 aacgcgtttc ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt
     9241 ttaaaagaat attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg
     9301 catctcatgg agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag
     9361 atatcacgct atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt
     9421 atttagaaga caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc
     9481 cgcaatccaa tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa
     9541 aaacagtaac ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat
     9601 tgattttgag ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa
     9661 atgtctactt gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc
     9721 cggctattaa aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag
     9781 caagccgaaa agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca
     9841 cttatttaga tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg
     9901 aagttttgac aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca
     9961 aaacaagtga aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa
    10021 atacagaatg gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg
    10081 aatacttgca aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt
    10141 ctatcccagc acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac
    10201 gttatgtagt aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag
    10261 gttcctctgc attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta
    10321 cagcagcaaa agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa
    10381 aatatgtacc agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa
    10441 tgacccatca attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa
    10501 atgtattggc actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact
    10561 tcttcttcca gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag
    10621 cgttaggaga agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa
    10681 aaagctgcct tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta
    10741 acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag
    10801 ggactgtgac acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg
    10861 gaggtcggta ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga
    10921 ttatttggat ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa
    10981 atgcttaaaa ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta
    11041 agaaaccgcg gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga
    11101 ataaaagtaa cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAZKNH010000085        10957 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000085 DAZKNH010000000
VERSION     DAZKNH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.085"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1020
                     /locus_tag="ACG8MG_002100"
     CDS             <1..1020
                     /locus_tag="ACG8MG_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFQ0376124.1"
                     /translation="DETLKNVYSSLIVSNIEETSSSEIKNKLSLLKLKEKEYIIDDAV
                     SVPLILNKREKNNLKKKMLIPFDSEVKGLLNKLTISNIDSTREKIIKIFDKINNLFKN
                     DNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKVRREIDREKKRI
                     DKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRENVLQRELNT
                     RAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPS
                     LETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLAKAEQYRRSL
                     ELSKD"
     gene            1092..2213
                     /locus_tag="ACG8MG_002101"
     CDS             1092..2213
                     /locus_tag="ACG8MG_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0376125.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(2295..4067)
                     /locus_tag="ACG8MG_002102"
     CDS             complement(2295..4067)
                     /locus_tag="ACG8MG_002102"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0376126.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(4070..5782)
                     /locus_tag="ACG8MG_002103"
     CDS             complement(4070..5782)
                     /locus_tag="ACG8MG_002103"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0376127.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            5897..6565
                     /locus_tag="ACG8MG_002104"
     CDS             5897..6565
                     /locus_tag="ACG8MG_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFQ0376128.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(6632..7420)
                     /locus_tag="ACG8MG_002105"
     CDS             complement(6632..7420)
                     /locus_tag="ACG8MG_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0376129.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(7503..8975)
                     /locus_tag="ACG8MG_002106"
     CDS             complement(7503..8975)
                     /locus_tag="ACG8MG_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0376130.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(9105..10298)
                     /gene="metK"
                     /locus_tag="ACG8MG_002107"
     CDS             complement(9105..10298)
                     /gene="metK"
                     /locus_tag="ACG8MG_002107"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFQ0376131.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(10296..10375)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            complement(10564..10635)
                     /locus_tag="ACG8MG_002108"
     tRNA            complement(10564..10635)
                     /locus_tag="ACG8MG_002108"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10600..10602),aa:Glu,seq:ttc)
     gene            complement(10647..10720)
                     /locus_tag="ACG8MG_002109"
     tRNA            complement(10647..10720)
                     /locus_tag="ACG8MG_002109"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10684..10686),aa:Ile,seq:gat)
     gene            complement(10733..10803)
                     /locus_tag="ACG8MG_002110"
     tRNA            complement(10733..10803)
                     /locus_tag="ACG8MG_002110"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10769..10771),aa:Gly,seq:tcc)
     gene            complement(10808..10880)
                     /locus_tag="ACG8MG_002111"
     tRNA            complement(10808..10880)
                     /locus_tag="ACG8MG_002111"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10845..10847),aa:Phe,seq:gaa)
     gene            complement(10884..10956)
                     /locus_tag="ACG8MG_002112"
     tRNA            complement(10884..10956)
                     /locus_tag="ACG8MG_002112"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10921..10923),aa:Asp,seq:gtc)
BASE COUNT         3641 a         2220 c         1814 g         3282 t
ORIGIN      
        1 gatgaaacat taaaaaatgt ctattcttca cttatcgttt caaatataga agaaacttca
       61 tcttctgaaa taaaaaataa gctttctttg ttaaaattaa aagaaaagga atatattatt
      121 gacgatgctg tttctgtccc tcttatttta aataaacgag agaaaaataa tttaaagaaa
      181 aaaatgctca ttccatttga ttcagaagtg aaaggattac tcaataaact aactatttct
      241 aatatagact cgactaggga aaaaatcata aaaatatttg ataaaattaa taatttattc
      301 aagaatgaca atgctgaatt aaggaagggt cttttagagt ttaaacttca agaactagaa
      361 cttcattact cttatttagt gaaaattacc gaagaaaaag aacaacaaaa agcaataaaa
      421 gaacagatga tagaagagga aaaagttcgt agagaaattg atcgtgaaaa gaaacgtatt
      481 gataaagaac aacgtcaatt caattctgaa atttccaaat taatcgcata tatgcaaaaa
      541 gctaatgcag atgtggaaaa agaattatat gcaaataaaa ttcaagaact ggaagaaaaa
      601 ttaaaagaat tagaagttat acgtgaaaat gtattacaaa gagaattaaa tactagagcc
      661 ggttatgtgt atgtaattag taatattggg tcttttggtg aagacatata taaaattggt
      721 atgactagaa gattagaacc tatggataga ataaaagaat tgagtagtgc atcggttcca
      781 tttgaatttg acgttcatgc gatgattttt tcagaggacg ctccgtcttt ggaaactaaa
      841 ttgcataatc attttagaaa acaagaagtt aataaaatta atcaaagaaa agaatttttc
      901 aaagtttcac tagacgaaat agagaaagtt gttttagaaa attataatgg aacagtaaca
      961 tttacaaaat tagctaaagc tgaacaatat agacgatctt tagaattgtc aaaagattaa
     1021 atatataaaa agccttcggg ctttttcttt taacaattaa taggaacgta tattcgaaag
     1081 gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa atttaagtac accgaacgtt
     1141 atacggatcc cttaactgaa aaaaagagaa aagtgtccgt tactttatct agcaattcac
     1201 gccaagcttg gaatcaagct aacttacttt tgaatgaaaa aatcgcagaa aaaataaaac
     1261 gaaatgaaga attaccacta acttttggag aattaaaaac taaatgggat gaaaaataca
     1321 agccaactgt aaaagaaagt tcctatcgaa caactcaggt ttatctatca ttaatttcaa
     1381 aatatataaa agatgatgta cttgtaaaaa acgttaatag taatcttata caagatatgc
     1441 tagattacta ctattttgaa aaaaatttat catataatta tgtaatacat ttaaaaactt
     1501 ttacaggaat gatttttaaa ttcgccaacc gtcgatatgg attaaaatat aatcccgtaa
     1561 atggggtttc tttagcaaga aaacctaaga caaacgaaga aataaaaaaa gaaaaagatg
     1621 gttatttatc aaaagaagaa gttcaaatga ttgctagtaa acaaaaaagt tcacatcaac
     1681 agtcacgtta ttctttaatt actaaatttt tgtttttaac tggactacgt tatggtgaat
     1741 tgatatcttt gactgaatct gattatgatg gtaataaaat tacggtttct ggtacttatg
     1801 attacgagtt aaaaattaaa acaacaacta aaaatacagg ttcttataga accattgaat
     1861 tatcaagtaa tgctaaagaa atcatagatc aattaattga agaaaacaaa ttaattaaga
     1921 ataataaaga taaatacatt ttcatctcta aaaatggtaa cccgatatct attcaagcat
     1981 ataatcaatc tttaagagca gtatccaaag aattaaacct agataaaaaa gtttcttctc
     2041 acatgttaag gcattcccac atttctctac ttacagaatt aggaatccca ttaaaagcca
     2101 ttatggatcg tgtaggacat gaagattcaa aaacaacatt aaagatttat actcatacaa
     2161 ctaaaaatat gcaaaatcaa ctagtagaaa aactaggaaa aatagaaatt taaattgccc
     2221 cttttctgcc ccttataaaa acaaacaaca taaaatatat aaaaagaacc ccgttataac
     2281 gaggttcttt taatttaact ttcaaataca aattgattat gataaagttc cgcataaaat
     2341 ccattttctg ctaataactc gtgatgactg ccttcttcaa tgacttcccc atcacgcaag
     2401 acaacgattc tatctgcatt caagattgtt ttcagccggt gagcaatcac aaagctggtt
     2461 cttcctttga ttgcttcatc cattgctttt tggatccttg cttctgttac cgtgtctaca
     2521 ttacttgttg cttcatccaa aatcaataaa gatggatttg tgatgatcgt tcgagcaata
     2581 ctgatcaact gcttttgacc tgtactgaat aagttatttt cttcggatat ctctgtatca
     2641 taaccttttt ctaacgtcat gatgaagtca tgaatattcg cttgttttgc tgcattgatg
     2701 acttcttcat ctgaagcgtc cggtttgccg aatgtgatat tgtctttgat cgttccggag
     2761 aacaatacag attcttgcaa aacgattcct acatgtctac gtaatgcatc aagatcataa
     2821 tctcggatat ctattccatc gatgactact gaaccatcat tgacatcgta aaaacggttt
     2881 agcagattca tgatcgtcgt tttccccgat cctgttggac caacaagagc aaccatttct
     2941 cctttgtcca cattgatcga gacatctttc aagactagtt tttctggaga ataaccaaaa
     3001 tctacgtgct gcaacgcaag acctttttca acaccatcaa atgctttaca gttttctggt
     3061 ttgacttcat ctttttcatc aaatatttca ttcaatcgtc ttgcccctgt aatcgctaat
     3121 tggatcatgc tgtatccaga tgaaatctgc atcagtggtt ggtaatattg ttgtgaatac
     3181 tggacgaatg tcacaactaa gcctaatgcc accgaacgtt ccaaatcacc atttaatgct
     3241 aaccagccgc cgaaaaagat aactatcgct gtattgacca gtgacattcc ttgcatcatc
     3301 ggaaatagta gaccagaata agcttgtcct ttgaaggttg cttcgcgaac actttgatta
     3361 tgctctaaga atccttcaat cgtttcttct tgtaagccat ttgtgatgat gacacgttgt
     3421 ccgctgattt tttcatccat ataaccattt agtttcccca cttcatcttg ctgaagatcc
     3481 acatatttcc gtgctttgcg gataatcaag acagctatca aaacggctac tggagtggaa
     3541 gcaattgtag cccaagctaa ctctacattt tggcggaaca tcatgatcaa gatcccgaca
     3601 agtaaaacag cattcgtgat gacttgaagc aaagcttggt tcaaactatt ttgaatatta
     3661 tctaagtcac tggtaaaacg acttaaaatt tcaccgtctt gatgagaatc aaagaaacgg
     3721 atcgtcaatt tttccaattt gttgaataga ccgatacgca ttcggttcgt tgacttccct
     3781 actacttgtg tgaacaaaat actatagata aagttggctg cactagctag tacataaaac
     3841 agcaagagtt tccatattac agaaacaaat ttgcttttat cgtctacgcc ttgcatcaag
     3901 cctcctacgt aattagctaa ttcttgaaaa gcttccccga tgtattgagg tgcctttact
     3961 tgtagatagg tagcaatgat cactgtgata aagataaaga aaaaggagag tttgtaccgc
     4021 ttcaaatagt gatagaaaaa tttacttgct tttattagat cagtcatact tactcctccg
     4081 ttcctttttg agtttcaaag atttcctgat agacacggtt tccagctacc aattcctcat
     4141 gggttccttc gccgactaat ctgccttcat ccaatactaa gatgcggtct gctttgacaa
     4201 cagaagcaat tttttgtgcg atgacaatcg tcgttgttcc cttcaaatct cgatcgagtg
     4261 cttctcggac cagcttttca gagcgagcat ccagtgcact cgtactgtca tctaggatca
     4321 agatctttgg attaccgata acgccacgag taatggataa acgttgcttt tgaccaccag
     4381 aaaagttact gctacgttct tctattggtg cttcgtaacg atcagataac ttttcgatga
     4441 attcttttgc ttgagcaata gaagaagcat gttccatctc tttttctgtt gcattctttt
     4501 tcccttggcg caagttttga gcaatcgttc ctgagaataa aattgctttt tgcaaaacaa
     4561 aggaaaccgt ttgtcttaaa ctcttttcat ttacttgacg aagatctaca ccacctactt
     4621 caactttgcc ttctgtcgga tcaaatagac gagggatcaa ttgtgctagt gttgacttcc
     4681 cggcaccagt cgccccgacg ataccaacca tttctccagg tttgatagaa aagctgatat
     4741 ctttaagcgt atctgtatcc tcaccaggat aacgaaaaga aacatggtca aaagtgacag
     4801 aacccgtcaa ctcttgttct ggaacgtcta aataagtaag atctggttca gcatctaaaa
     4861 tttccgcaat ccgcttcagc gatacggctg ctcttgaagt catcatcatc atcattccgc
     4921 caataatgat tgccatcata atctgcatca agtagttcat gaatgaagca attccaccaa
     4981 ttaacgtagg atcatcttta acaagatcgc tcacaaaaaa gatcgcccca accacagcca
     5041 aattggcagc cagcatgaag gacgggatca tcacagaaaa taatgtaccg actatgatgt
     5101 tgtgttttgt taagtcctca cttactttgc tgaatgactt caattggttt tcttcttgta
     5161 caaaagattt cactacacga atccctagta agttttcttt tgcaatgcta ttcacacgat
     5221 caatcagttt ttgaatgatc atgaagtgct tgcccatcct tgtaaatgaa agtgccgtaa
     5281 tcagaaatac cgtaacgata agcgctacga tgatccacca taattgcggc atcgtgatca
     5341 tagctaaaat gaaggcacca ataaataaga atggaattcg aaaaagtgat tgcaaagaaa
     5401 tcatcactaa attttggatt tgcgtgatat catttgttag acgaacaaca agatttccag
     5461 cagagaactg ttcgatattt ccaaatgaaa atgtctggat ctttcgaaat gcttcttctc
     5521 ggatatctgc acttacgcct tgagctactt ttgcagagaa aatcgtgttc gttacacctg
     5581 cggctaatcc tagcaaagcc agactgatca ggtaaatacc aattgttttc atttcatcat
     5641 tatcttctgt aataatagct tccaatactt gctgtagtaa cttaggttgc cataatgctg
     5701 ctgcaaccat gactgctacg gacagcaagg aaataaaaac agctacttta tattttttcg
     5761 catgtcgtaa gattaatttc attcgttgat tacacccctt tgtttaaatt gacaggacta
     5821 atttaacact gtaaaattag accgactaac aatttaccat gtttaaaaag ataatgcaat
     5881 acacgaggtt gataaaatgg aaataaagct ttctcaagca atcatcattc aggccgcctt
     5941 tgatatcctg gctgaaacac aagctctttc gcagctttcc atgcgaaaca tcgcaaacag
     6001 aattcatgtt caagcacctg ctttatattg gtatttcaaa aacaaacaac agctcttgca
     6061 aataatggct gaaacaatgg aagaagaatt agcgcttcca gatccaactt tgccttggaa
     6121 tgaacagttg ctgcagtata tggaaaatta ctatgacctt tatacacgtt tcccttgtgg
     6181 ggcagaatta gaaatcaata ctgtgcccgc ttttccgagt cgattagaac atttggagca
     6241 aatgatccaa ttacttgttc gtgaaggatt ttcagtcacc caaagtcatc aggccattgc
     6301 ctctctccac aatttattga ttggacagtt aatggatcag cagcatgaac agcagcttcg
     6361 acaaaaaatt atggacggaa atgaattttt gaaagaaaca gttctattta tgcgtaatta
     6421 tgtagttgaa aatcaattag agggatttaa agaaagtatg cagtcccatg cagaattgaa
     6481 tgaaaaagca ctctttctaa cgagtgcaca aacctatatt gaaggcttat gccaacaaaa
     6541 taaaagaaga gaaaacgagc agtaaaaaag ctcctataga gatcactatg tttgtgatct
     6601 ctataggagc tatcttttta agactctttt tttatttttc ttccattagc cacgccaatg
     6661 cacgttgaat ccattggtcc cagaagatcc agttatgatc gccaggtcct tgttcaaagg
     6721 tgaccgccaa gccttgttcc tgcatagcag ataccatttc ctgatttgcc tgataaagaa
     6781 aatcagattc cccacaagcc atataaaatc ttggaactgg ttttccgctt tttactagtt
     6841 ctttcaataa gtattgcgga tcattttttg aaccttcgat ttgatccaat ggtccaaaga
     6901 tcccttccca aaaagatgct ttacgaacca tcaaaagatc atccattcga tttcctacag
     6961 aaacagcgcc tgaaagagag attactgctg cgaaattttc tgattttgca aggcctagtt
     7021 ttaacgcgcc atatccgccc atcgataatc cagcggcata tgttttttct ctttttcttg
     7081 ttaattgcgg gaataattca tgacaaatct caggtagctc ttctgcaatg aatgtccaat
     7141 acttcatgtc ataaactgta tctgtgtacc agccaagatc agtagatggc atgatgacag
     7201 ctaatccgta gtttgctaca taacgttcaa tcgacgtacg tctttcccat acactatgat
     7261 tgccacccat tccatgaagt aaatataaaa caggtatatc cgttgtctct tgctctgaac
     7321 tggtgccaat cacctttttt gttgtttgcg gtagtatcac atttagattg acttccattt
     7381 ctaacacatt cgaataaata tttgcttgaa taaatgccat attttcgcct tccttagtaa
     7441 gaagttattt ttgaataact ttaaatttga atgattcggc tacgtacata tcttaaccga
     7501 aactactgtt ttattttacc acttttttca tgaacgaaaa gcgttaagac caaagcaatg
     7561 atcacgagta acaagccaac tagataaacg gaatgtaacc catcataaag aatttttctt
     7621 aatggtaaca aaatatccgc atcaatatat ttagccgttt gtggattcac cagtttattc
     7681 atgatatctg catcatttgt taaatttgct gctgcaagtt ctcgtttagt taccccattt
     7741 atcaataaac caaaaataga aaccatgact gtttgaccaa ttgttcgaac taacgtatta
     7801 aaagaagttg ctacaccaag ctggtcacga gatacactat tttgtgcact gacagtcgag
     7861 gtagtcatgg tcacacctaa gcctataccg atgaccgttg atatagcaaa aaaccataaa
     7921 tagttacttc tgaaaggcat aagatacaac caagcgcccc caatagctgc gattagtagt
     7981 ccaattctca agacccaatg agttttcatt tgagtcatcc acttgcttgc aaggaaagaa
     8041 ccgaccatcc agactagtga taatggtgct aataccagcc caccaattgc tgcattcagc
     8101 cctaatacac cttgcatcca catagggata taaacatcga ttcccatcaa aaaaccacta
     8161 gccaatgctg cagccaagtt gaccacaaca aatagtggat ttttgaataa atgcatcatg
     8221 atgacaggat ctttagctct tttttcggca aaaacaaaaa tacctaacat tacgatacta
     8281 cctagcagca aagcagaaac aagcggactc attccttcgt ctcctaataa ttggaaccct
     8341 aacagcaatg agacaagcat ggccatcaaa ctaaaactac caaaaatatc cattggttta
     8401 gactcatgta tgatttttgg ttcatttaaa tatagccaaa tcaaaataat taatagaatt
     8461 ccgatcggca cattgataaa aaagatccag tgccagctga tcgtttcaac gatgaatcct
     8521 cccgcaagcg gtccaaaaac actggcaatc ccccaggctg aactattcaa acctaaaact
     8581 tttgctcgct tttcaataga ataaagatcg ccgatgatcg tcaaagctac tggcatcatt
     8641 gccccagcac ccatcccttg aatcgcacga gcagcaatca acccaatcat gctttgagat
     8701 aaaccgcaaa ataacgaacc gataataaac aataatgtcc caaaaataaa gattggtttc
     8761 cgtcccactt tatccgcaag ttttccgtaa atcggagtaa gcatagcgtt tgtcagcaaa
     8821 taaatcgaaa atacccagtt catgatttcc attccatgta atgaaccgac aatcgttggc
     8881 atagcggttg ttacgattgt accttcaata gccgtcatga acgttgcaat gaaaatactc
     8941 actgtgacca aagtaacatt tgtttctttt ttcatcttat tcctcccttt cattatgaca
     9001 ggatctaaaa atagataaat aattactatc tttttcaaga ggttaactgc cataaaaaaa
     9061 gctggaacaa ccaccacttg gcgatcgttc cagctgaata taacttattt tgctaaactt
     9121 gctttcaacg catctacttt gtccgtatgt tcccaaggca aatctacatc tgtacgacca
     9181 aaatgaccgt aagcagctgt ttgtttgtaa atcggacgac gcaaatcaag catctcgatg
     9241 attcctgctg gccgaagatc gaagttttct ctcactgcag caatcagttc ttcttccgga
     9301 accgttccag taccgaaagt attgattgag atcgaaacag gttgagcaac accaatcgca
     9361 taagctagct gtacttctgc tttgcgagca agccctgctg caacaatgtt tttagcaata
     9421 taacgtgcag catagctggc agaacggtca acttttgtcg catctttacc agaaaaagca
     9481 ccgccaccat gacgagcata accgccatac gtatcaacaa tgatttttct tcctgttaat
     9541 ccggcatccc cttgaggacc accaatgaca aatcggccag tcggattgat ataatatttc
     9601 gtttgatcat ctaataattc agctggaata acttccttca ctactttttc gatgacatca
     9661 tgacgaattg tttcattatc tactgcatca tcatgctgtg ttgaaataac gattgtatct
     9721 acgcgttccg gttgtccttg atcatcatat tcaaccgtta cttgagattt tgcatctgga
     9781 cgtaaatacg ttaattcatt tgatttacgc aaatctgcca agcgtcgtac cagacgatga
     9841 ctcaaagcga ttggtaaagg catcaattct ggtgtttcat ctacagcaaa cccaaacatt
     9901 aaaccttggt caccagcacc aatctcatct aatacgtctt ttttatcttc atcccggatc
     9961 tcaagtgctt catcaactcc ttgagcaata tcaggagatt gttcatcaat cgcaaccaat
    10021 acagctgctg tatctccatc aaatccgaat tttgcacgtg tataaccaat ttcttttatt
    10081 gtttcccgta cgactttttg aatatctaca tatgccgtcg tagatatttc tccaaatact
    10141 aaaacaagac cagttgttac agacgtttca cacgctactc gtgctgttgg atcttgtttt
    10201 aaaattgcat ccaagattgc atcactgatt tggtcagcaa ttttatctgg atgtccttca
    10261 gaaacagatt ctgatgtaaa taagtgtctt tctaccatta taatgattcc ccctagttaa
    10321 attcggttac aaggcatctc cgtcctgcgg atcctttcgg gaacttgcaa cgaaccaagt
    10381 atagcagata aaacagtttt ctgctcgtat tttttcattt ttttgcttaa aaaatataaa
    10441 aatattttac attagaactg tccaaaaaag cttagcacaa tcatccataa agataactct
    10501 gctaagcttt ttaaaatcat tctttcagat aaaaagaaaa agatactcaa aaagtatctt
    10561 ccatgacccg tacgggattc gaacccgtgt taccgccgtg aaagggcggt gtcttaaccg
    10621 cttgaccaac gggccaataa aataaatggg cctaaatgga ctcgaaccat cgacctcacg
    10681 cttatcaggc gtgcgctcta accagctgag ctataggccc ataaaaacta aaagcgggtg
    10741 acgagaatcg aactcgcgac aacagcttgg aaggctgtgg ttttaccact aaactacacc
    10801 cgcataatgg cgcgggacag aatcgaactg ccgacacatg gagcttcaat ccattgctct
    10861 accaactgag ctaccgagcc aaacggttcc gacgggaatc gaacccgcga tctcctgcgt
    10921 gacaggcagg catgttaacc cctacaccac ggaacca
//
LOCUS       DAZKNH010000086        10954 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000086 DAZKNH010000000
VERSION     DAZKNH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.086"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..388)
                     /locus_tag="ACG8MG_002113"
     CDS             complement(164..388)
                     /locus_tag="ACG8MG_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFQ0376132.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="ACG8MG_002114"
     CDS             complement(586..2166)
                     /locus_tag="ACG8MG_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFQ0376133.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="ACG8MG_002115"
     CDS             complement(2567..3943)
                     /locus_tag="ACG8MG_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0376134.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5159)
                     /locus_tag="ACG8MG_002116"
     CDS             complement(4071..5159)
                     /locus_tag="ACG8MG_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0376135.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="ACG8MG_002117"
     CDS             5331..5765
                     /locus_tag="ACG8MG_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0376136.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="ACG8MG_002118"
     CDS             complement(5843..6376)
                     /locus_tag="ACG8MG_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFQ0376137.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG8MG_002119"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG8MG_002119"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFQ0376138.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG8MG_002120"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG8MG_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFQ0376139.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG8MG_002121"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG8MG_002121"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0376140.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="ACG8MG_002122"
     CDS             complement(10101..10679)
                     /locus_tag="ACG8MG_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFQ0376141.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         3234 a         2226 c         1865 g         3629 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgc
//
LOCUS       DAZKNH010000087        10651 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000087 DAZKNH010000000
VERSION     DAZKNH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.087"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..657
                     /locus_tag="ACG8MG_002123"
     CDS             34..657
                     /locus_tag="ACG8MG_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376142.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /gene="trpX"
                     /locus_tag="ACG8MG_002124"
     CDS             1107..2156
                     /gene="trpX"
                     /locus_tag="ACG8MG_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0376143.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="ACG8MG_002125"
     CDS             2153..3034
                     /locus_tag="ACG8MG_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0376144.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="ACG8MG_002126"
     CDS             3037..3807
                     /locus_tag="ACG8MG_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0376145.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="ACG8MG_002127"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="ACG8MG_002127"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFQ0376146.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="ACG8MG_002128"
     CDS             5118..6281
                     /locus_tag="ACG8MG_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0376147.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="ACG8MG_002129"
     CDS             6398..6808
                     /locus_tag="ACG8MG_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0376148.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="ACG8MG_002130"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="ACG8MG_002130"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0376149.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..9665
                     /locus_tag="ACG8MG_002131"
     CDS             9381..9665
                     /locus_tag="ACG8MG_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376150.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9825..10061
                     /locus_tag="ACG8MG_002132"
     CDS             9825..10061
                     /locus_tag="ACG8MG_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0376151.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10058..10456
                     /locus_tag="ACG8MG_002133"
     CDS             10058..10456
                     /locus_tag="ACG8MG_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0376152.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3722 a         1773 c         2253 g         2903 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9721 ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata
     9781 atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa
     9841 agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag
     9901 aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg
     9961 tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga
    10021 atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga
    10081 aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt
    10141 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
    10201 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
    10261 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
    10321 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
    10381 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
    10441 aaccgcaata aaatgaacga attaaaaact ttatttctca attagatcag catgtcatta
    10501 aggaatatgc tgattttttt atgtttaatc atacaaaata gttttttcat aaaaatatgg
    10561 aaaatatgga ttaattttga aataattgat ttaaaaggaa tacaaaagag tgaatagtag
    10621 gaaatagtgg tttagaacta gtgggctgat t
//
LOCUS       DAZKNH010000088        10340 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000088 DAZKNH010000000
VERSION     DAZKNH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.088"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(364..1482)
                     /locus_tag="ACG8MG_002134"
     CDS             complement(364..1482)
                     /locus_tag="ACG8MG_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376153.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1715..1837
                     /locus_tag="ACG8MG_002135"
     CDS             1715..1837
                     /locus_tag="ACG8MG_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376154.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1953..3260
                     /locus_tag="ACG8MG_002136"
     CDS             1953..3260
                     /locus_tag="ACG8MG_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFQ0376155.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3379..4578
                     /locus_tag="ACG8MG_002137"
     CDS             3379..4578
                     /locus_tag="ACG8MG_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0376156.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4605..5873)
                     /locus_tag="ACG8MG_002138"
     CDS             complement(4605..5873)
                     /locus_tag="ACG8MG_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFQ0376157.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(5928..5994)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6036..6682)
                     /locus_tag="ACG8MG_002139"
                     /pseudo
     CDS             complement(6036..6682)
                     /locus_tag="ACG8MG_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7090..7593)
                     /locus_tag="ACG8MG_002140"
     CDS             complement(7090..7593)
                     /locus_tag="ACG8MG_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0376158.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7716..8480)
                     /locus_tag="ACG8MG_002141"
     CDS             complement(7716..8480)
                     /locus_tag="ACG8MG_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFQ0376159.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8588..8863
                     /locus_tag="ACG8MG_002142"
     CDS             8588..8863
                     /locus_tag="ACG8MG_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFQ0376160.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9301..10251
                     /gene="yidC"
                     /locus_tag="ACG8MG_002143"
     CDS             9301..10251
                     /gene="yidC"
                     /locus_tag="ACG8MG_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFQ0376161.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         3118 a         2170 c         1878 g         3174 t
ORIGIN      
        1 taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcaca aaaatttgag
       61 aaataatttt tgtcaatctc ttcttatttc ttgaagctga ccacttctgt cacaacctct
      121 tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaccaaa caaaaaatat
      181 gaagagctat ttttgcttgc tgttcttatt acttgatgca gaacagcttt ttcacaacct
      241 ctcatacacg gtgtttaatc agctgacctt cggtattagc tcaaaaaacg gaaaaatatg
      301 tgaaactatt tttccgtttg tttctctaat actcaggtca aaacactgat ttcacaacct
      361 cttttaataa cgaagaatcg ctgtcagact cttttcatct ggcgcatctt tttgatccaa
      421 aataaacact ttcccgccat taatgatcac atcatctgct aatgcattca gcacttgacg
      481 ccgatcatat tctgtttcag gatcttcacc gaaaccatca gctaagtttg acgtagcgat
      541 aaataaatgt gacacttttc cttctttcgc agctggaacg atatcaacta actgatcgat
      601 gaatttctta tctaagagtt tattgtattc ttttgattct tgagctgcga gttcacttga
      661 cagcttcttc gccccttctt cgatatctct aaaggatagc tgcgcaggag aagcagaaat
      721 cgcaacgtct gcacaataat agggattttt agcaattttt ttgaacatcg tctgattttc
      781 tggtaatgca aataaataaa tcggccattg ttcaggattt tcgaaagaat ccttcaagta
      841 cgtatcaaca gcttggtaat agttgaccca atcgatctct acttcttcat cttttggatt
      901 tacgccatgg aaagttactc catctcgggc tccgcctcct ccattagaag agaagttcaa
      961 accgccacct gtcagttcag atcccaatgc agtaacgaca tcaaccggtg ccccttctgg
     1021 tagagatact tcttttactt gtttgttgct gacctcatac aatttcatgg agtcgcggtt
     1081 caatcccaat aaataataag aatagttgaa ctgcccattt ttgataatag ccagtaaata
     1141 aggaagatca cttacatagt attgatcgtc taccgggata ctcaaacgat ggatataggt
     1201 ctggtcagca gttataatga ccgctacact cgtagttgca ctacgccaaa acgaaggatc
     1261 tcctaacaaa ttttcaattt tttcttggaa cggggaccag tcatgttcgg aatatttttt
     1321 ctcaaaacgt ttctttgctt ctttcgcaaa atttttgaat ttgatctgat ctttttccac
     1381 atcttgatgg gcgacatgcg tattcagcat gatcgtgacg aatggtcctt tagcgccttc
     1441 tgaagtcaat tgtgtcaata attctttatc cttttttgcc ataaacgtac ccctcctaaa
     1501 aaaatcattc ctttgtttct atctaaagtc taccacaaca ctatttttcc atacaataaa
     1561 acgtatctaa aaaaataaaa cggtttctac ggttttaaat ttagctattt ttttaagtta
     1621 tcttcataat ttcctcaatt atttagcccc agtcttcaac tattcttcat gaaatgctga
     1681 taaactaaga aaaagaataa aagaggtgga tcttatgcat ttgattatga ttttcagttt
     1741 attaagtatt atcagtttat ttgctttcag cgcttcccgc ttattagatg cggaaacaca
     1801 gcctattcct gtaaaagtac gtaaaaacga ccgctaaact tccataacgc gcgttcttat
     1861 cctaaagttt tttcagaaac attgaaactc tctcccctct ctgataaaat tttaatttgt
     1921 agcgaaacaa attaattaca aggagatcgg atatgtccat aaaaaaaaca cgtctagtcc
     1981 tattattggc tagtctcatt ctttgccttg cttcttttct accagctgtt gctgtgcaag
     2041 ctgaagatac gttcaaagta aatgcaaaag cagcttttgc tgtagatgcg caaagcggta
     2101 agattcttta tgatcaagat ggagaaaaaa ccatggggat cgcttcaatc acaaaaatca
     2161 tcggtcttta catcgtcctt gatcaagtaa aagaaggaaa actgtcatgg ggtgataaag
     2221 tttcgatttc agattatgca gaaaatttga gtatcacccc agatttatcg aatgttcctt
     2281 tgcataaaga aaacacctac actgtcaaag aattattcga ctctgcaatc atccagtcag
     2341 ccaatgcttc aatggtggcc ttggcagaaa aaatctctgg cagcgaagct aaattcactg
     2401 aacgaatgaa agaacaattg aaagattggg gaatcaaaga tgcaacaatc gttaatgcat
     2461 caggattgaa caactcttat cttggcgaaa accgaccaga aggcactgga gaaaacgacg
     2521 aaaatcaaat gtctgctcaa gatgtggcca tcgttgcccg ccatttgatc ttggatttcc
     2581 cagaaatact ggatgtatca agtactacga cccaaatgtt tggcgaaaat acgcaatcac
     2641 cagtggaaat ggtcaactgg aactggatgc tcccaggatt cgttaattac aaggaaggcg
     2701 tggacggatt aaaaactggt acgactgagc ttgcaggtgc ttgtttcgtt ggtacgatca
     2761 ctaaaaacga tcaacggatc atcactgtcg tattgaatgc ggacggccat gcggaaaacc
     2821 cgagtgcccg cttcaatgaa acagaccgtt tgatggacta ctgctatgac aattggtcag
     2881 aaaaagagct cggaaaagct aacgcaagta ttcctagtct aaaaaccatt gatgtaaaag
     2941 acggaaaaga atcaacagtt cctgttgtgc ttaaaagccc tgtaaaagta tgggtacgaa
     3001 acgatatgga tactggcaaa ttaacgatca cgccaacact tgacaagaag caagtcactg
     3061 acaatgagat ccaagcacct atggataaag gtacaaaaat cggtaccgct acgatcacct
     3121 tggcagacga taaattaggc tatttagaag acaacaatgc accatcaaca gagatcatta
     3181 cggataaaac agtggaaaaa gcgaatatat tcgtgattgg ctggcgacgc gtcgcagaat
     3241 ttttcggcaa cttgttttaa ttgaacaata agaaaaacat ctttccatcg gaaagatgtt
     3301 tttttgcttt ttctagttta aataacagct tttttcatct attttcgtta tactgtataa
     3361 gaaaaaggag ttgaatttat gaatctaacc agagatctga tctttttagt tctctatctt
     3421 attggggtat tctctatcgt ttattggcga aaacagaaga aggatccttc cttgttcttt
     3481 tacggcagta cgtggacatt tacggtaatc ttaacttatt ttgtcgtgtt ggaaattttt
     3541 tattcgacct tttatttttt gattccattg ttgttcttta gtatctttgc tttttcttat
     3601 ttcacagaaa aacgaaggct actgaatggt cttttattca atgttttact catcagtttc
     3661 ggcatctact tgtttgtctt gctgtatgaa acgcaaaata tttttcttgg cggactgatc
     3721 gccttgatca cgattccatt gctacttgtc cttttatttg gtatttatgg attgattgtt
     3781 tttctttttt ggaacggggt cacagtttta cgtagagaat cgcattcatt agctaatctt
     3841 ttaacgttga tactagccat ttttcttaca ctgtttcttg tttttgactt tttcttgtta
     3901 aaatatttgc cgcaatggat aaatgctttg tttttctgcg ttcccctgat cttgatctat
     3961 ctatttatcg tattctacaa ctttttgact gtctcattct tgtatcagtt caaccggcct
     4021 cgatacaatc aagacttcat cgttgtgtta ggcgctggat tgatcaatgg agaaacagtt
     4081 tctcctcttc tagccaagcg aatcaataag gcaattgctt tttatcgggc tcaaagccgt
     4141 gctacattaa atccgcccat cttattgatg tctggcgggc aaggagcaga cgaaaaagta
     4201 ccagaggcca ttgcgatgaa acaatatgcc atggaacaag gaatccctga aagagacatt
     4261 cttgtcgaaa ccaattcgac aaccactttg gaaaacatgc tctattcaaa ggaaatcatg
     4321 gatcagcaaa tgaaagggcc ataccgcgcg atctttagtt ccaacaatta ccacattttt
     4381 agagccggtc tttatgcgcg gcaagccaaa ttgaaagcaa atggtattgg tgcaaaaaca
     4441 gctttctact acttgcctaa tgcgttttta agagaatacc tcgccatctt gatgatgaac
     4501 aaaaaaagac atatgatcat ttgcggattg cttttcgcag gctccgcatt cttatcgctg
     4561 attactttac ttttctaaaa aaacaaggtg tgacaaaaca tatgtcacac cttgtttttt
     4621 tctttcattc tcttaactat tggcaaaaag agtatccctt ttttgaccgc tacttgctga
     4681 gcagaataaa taccgctgtt gcctgaacac ataaagctga tcaagcagat cataaagaag
     4741 tatactgccg cttgtgaacc gaacaattcg atccccatca caaagcaggc aatcggtgta
     4801 tttgtcgcac cagaaaatac tccgataaag cccaatcctg caagaaaagg aacagaaaca
     4861 tggagcaaag gcgctagtgc tgcaccaagc gttgcaccaa tttcaaacaa tggtgtcact
     4921 tctccccctt gatatcccgc gcctaaagac aaaactgtga agaataactt cccaataaaa
     4981 tcatagacat gtgcctgtcc attaaatgat tcttgaagta acggaagact caatccgaga
     5041 taacgttgtg tttgcaaaat caatgctgct gccacaacta cagctccacc tatcgtattt
     5101 cgacaaacag gatgaggaat ccattgtgca tatcgcgctt ttaaaaagac gatcgaacgg
     5161 ctgaataccc aaccaatcaa accaaaagca atcccagcaa caaaaacttt gaagaacagc
     5221 aacatactcc attcaggaat cacacccatt tggtaatgta gatgagaaac accatagctt
     5281 tctgtcatga aatttgcaaa cagtgcagca aagaagctag ggaagatcgc ttctgttcgg
     5341 actttcccga ttgccaacac ttctaatcca aataatgttc cagctaaagg tgtgccaaat
     5401 acagaactaa aacccgcact gattccactg ataatcaaga tttcccgttc tgatttatcc
     5461 aatcggaaca aacgtgccgt attatctgct aacgctcccc ccatttggac tgctgttcct
     5521 tcacggccaa cagatccccc aaataaatgg gtagttatgg ttccaaacag tgtaagtgga
     5581 ataagccgca acgggatttt ttcttctcca ccatttcctt gatcgatcac taagttgttt
     5641 ccacgactag aaagtccgcc gtgatacgca tagagataag caaaaagcgc accactaact
     5701 ggtagtagac aaaggagcca aggatgtgat agtcggatat ctgtcacaac tgtcaggcta
     5761 tttaagaaaa aagcagacaa gctccccatg aatataccga gaatcgctgt gatcaccagc
     5821 cacttcagca tatacactaa aatgcgtact ggactcggta attgtttttc cataaaaaat
     5881 tcctcctcaa taaaaaaacg cctactaaag aaaggggctg tccccagact ttagtaggcg
     5941 tcattagcta tctttcgtta gcatttaaag gcgaacacca tcacctattc acttccatta
     6001 aaagatagca tatttttctg taaaaaacaa taaaattatc gttctttctt tacacgttca
     6061 gcaaattcat agaaaggagc caatttcgtt aactgttgat tacattggtc actatcatga
     6121 caaatataat tccccttttt cgtatacgtg ccatcagaac ctgctttcgt tgtagaaaga
     6181 aaaagcgaaa cattcgatgt tttctgacag attgcacaga ttcccttatt tatcgttggt
     6241 gataaagtac cgctaatccc ttttaatttt tcatcttcgt aatagaccat gaattttttc
     6301 tgcgtacctg gatcattcca gccgatgtac gtatgttctt gcaagtcgat ttcattccaa
     6361 gctggttgtt tgagtttttt cacttttcgg aataatttct cgatttgtgg tttgctaggc
     6421 tgcataaacg gctgaacgat ttgcttcagc tcctccaatg ctctttctgc ttgcgctttt
     6481 gtcagtttta cgtctaaaac tgtcgttaaa aatgtatcga ttctggcagt tcgtgatcta
     6541 atatgtctcg aatcttttca gctgctagtg cctgaactgt ttgtaccgtt tttggatcat
     6601 tgacagatga gtacgcattt aataactgtt ctacttctct tattataaaa aaatattggt
     6661 atggttttat tgtttgttcc atgattttaa agtcctttct tctaaccaaa gtgcatactt
     6721 tgtagaaaaa agtgatcttc tgcaaagtat gtgttacctg aagatcccgg actcttttaa
     6781 ataagatacg ttttgcatct tatcatcacc ccctttaatg cttattattc tatgtacatg
     6841 attcattcga taaataaaag taaaccgtat cttgaacaga tagtcaactg caaacagaaa
     6901 aaaggactgt gacaaaagtc gtgtcaaagt cccttgtttc gaatgaatgg tgttcaaaag
     6961 ctggcatctg cggtaataag ccgaaacttc acaaaaattt gagaagcaat tttcgtaaat
     7021 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tcaatatggt ctattattgg
     7081 ttttatctat taaatcgagg attctttccc aaagtaacgt tgccatctct tacgcatact
     7141 tgctgacata ggctgagcgg cttctttgac tgcgcagtat ttgtctacga atgttacgat
     7201 atatccttct ttgtacttag gcggtgcaat cgttgcgccc cacatgtgct tcaaaatgat
     7261 atctcgctca agatccgaca attcggtgat cttttctgca ttctttacag caattcttgg
     7321 atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta cgccaatcat agtagaacag
     7381 atcatgcaaa agtccagcac gcgctgttgc tttagcattg ccaccccatt tttttgcgag
     7441 cagataacta tagtaagaaa cactgatgga atgctccaaa cgagtggaat gaacatgctg
     7501 tgtgtaattc gctaattttt tcactgcttc tgtttctaaa agatcttcta cataagacat
     7561 atattcttcg tcttcacgcc atttttctgt cataattatc gatctgccac ctttttgaat
     7621 ttcattttct tctgaaattg atctaagtat accaccagtg ttcggattat tctagtcgcc
     7681 ttggataaaa tagccaaagc ttaatagttc cttaatcata aagaaaaatg ataaagaaca
     7741 atccctgccg caaccccaac attcaacgat tccgcatttc ctttcatcgg gatatatacg
     7801 ttctgatcag tcaaagacaa gatttcctga cttaccccct gaccttcgtt ccccatgatt
     7861 attgcgaagt tatctgattt ttctactttg tctaatgcgt gtgcttcttc atttaattct
     7921 gtgccataca ctgtaaaccc tttgtctttc attaaagaaa tcacttctgt caaatctttt
     7981 tgcaacacag gcaaatgata attgcttccc tgcatgctgc gcagcacctt tgtactataa
     8041 atatctgccg ttcctttgcc taataaaaca ccagccatat caaatgcatc tgccgtacgg
     8101 atcatcgtcc ctacatttcc aggatcttgt acattatcta acaacagcca tcctcctgaa
     8161 aaatctggcg tgccttcagc cgttaacgcc atcactgcta agattccttg tggtgtaggt
     8221 agatccgata cagcatccat gacctcatcc gaaactagaa aagtagaaat ctctttgttt
     8281 gcggccagcc agtctcccca ttctttcatg cctcgctgtg tcacaaaaag ttcttttatt
     8341 tctgcatcag acgacgcagc ttcttcaatc agatggaacc cttccaaaag atattcttcc
     8401 ttttcttccc gatatttctt tttatgtagt ttttttaatt ctttgattct tggatttttt
     8461 gctgattgga tctctttcac attcctcacc tccccatgat tatatcatgg aataggtgct
     8521 tgagcttgtt tcatcctaaa aaaatcggta tcattaattt ataaaatagg aaagaaaggt
     8581 gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc gtccaaggcg taggatttcg
     8641 ctacatgacc aagatggtcg ctgatcaact aggcgtgact ggaacggtaa aaaacgaaga
     8701 tgacggctca gtcacgatcg aagcaatcgg caatgacgac atcatccaga aatttattga
     8761 agaagtcaag aaatctccga gcccaagtgg acgagtgcag tatgtggatg tccaagaaga
     8821 cccgatgatt gaggaacgaa agaaatttga tgtggtggga taaggttgtg aaaaagctgg
     8881 tctgcatcaa gcattaagga caggttgtga aaaatagctc tccatatttt tcaacaggct
     8941 ggacttaatg ccgtagatgc aagcttttga acaccgtcga taaggttatt cttgaggcgc
     9001 tttgcctcaa aaattaagga cagcagtgaa aaagctgatc tatcacttgc agtgagttca
     9061 tttgactccg ctttttcggg cagaaaaaaa gtttttagtt tattaaagta agaaagaaac
     9121 acttggaatc attctacttt ttcctagtat ttctttcttg attttttgtc tttttttcaa
     9181 aatttttcgc tacgtaaaag aacgaatatt tatatgaaat ttctatgaag ttgattgaat
     9241 tccatgcgct tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct
     9301 atgaataaat ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta
     9361 tctggctgtg tgcggatgga ttcaaacgga aaccctgaca cttcgggaat cgtttatcga
     9421 gtactcgtcc atcctatggg ccaagcgatt acatatctag ttgaaaattt caattggtct
     9481 tatggctggg cagttattgc aatgactgtg atcgtccgta tcatcatctt gccactaggt
     9541 attagtcaat cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa
     9601 gttgaagcag cccaacaaaa attgaaagct gcaacaacac gtgaagaaca aatggcagca
     9661 caagcagaaa tgcagcaagt ataccgcgaa aacggattga gtatgaccgg cggaatcggc
     9721 tgtctgcctc tattgatcca aatgccgatc ttctctgcct tgtactttac ggcacgttat
     9781 acagaaggta tccgggaatc atctttctac ggcatcgatt taggctcacc aagtatggta
     9841 cttgttgcca ttgcaggggt cgcttatctg cttcaaggat acatctcaac gatcggtatt
     9901 cctgaagaac agaaaaagac gatgcgtacg atgctgatcg tctctcctgc tatgatcgtc
     9961 ttcatgtcga tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc
    10021 agctgtctgc aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg
    10081 gcagaagagt tgaaacaaaa cccaccaaaa caagtcgtca ctccaagaat tgatgtgaca
    10141 gaatcagcta aaccagctga cagcaaacaa gcaaccaaac aaatcaagca acccaaaaac
    10201 agtacaaaca ataaaggccg caacgcagga aagcagcaga agcggaagta gatagtaaat
    10261 aagagtgtaa taaaaattca ttaacagttt tagcaattaa aaatatcgac caagattttt
    10321 gaaaaagaaa tctggtcgat
//
LOCUS       DAZKNH010000089        10277 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000089 DAZKNH010000000
VERSION     DAZKNH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.089"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..236)
                     /gene="acpP"
                     /locus_tag="ACG8MG_002144"
     CDS             complement(<1..236)
                     /gene="acpP"
                     /locus_tag="ACG8MG_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0376162.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHL"
     gene            complement(358..1359)
                     /gene="plsX"
                     /locus_tag="ACG8MG_002145"
     CDS             complement(358..1359)
                     /gene="plsX"
                     /locus_tag="ACG8MG_002145"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFQ0376163.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(1408..3444)
                     /gene="recG"
                     /locus_tag="ACG8MG_002146"
     CDS             complement(1408..3444)
                     /gene="recG"
                     /locus_tag="ACG8MG_002146"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFQ0376164.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(3561..5243)
                     /locus_tag="ACG8MG_002147"
     CDS             complement(3561..5243)
                     /locus_tag="ACG8MG_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFQ0376165.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(5295..5657)
                     /locus_tag="ACG8MG_002148"
     CDS             complement(5295..5657)
                     /locus_tag="ACG8MG_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0376166.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            5927..6115
                     /gene="rpmB"
                     /locus_tag="ACG8MG_002149"
     CDS             5927..6115
                     /gene="rpmB"
                     /locus_tag="ACG8MG_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFQ0376167.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            6353..9022
                     /locus_tag="ACG8MG_002150"
     CDS             6353..9022
                     /locus_tag="ACG8MG_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFQ0376168.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(9477..10223)
                     /gene="truA"
                     /locus_tag="ACG8MG_002151"
     CDS             complement(9477..10223)
                     /gene="truA"
                     /locus_tag="ACG8MG_002151"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0376169.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         2794 a         2223 c         1851 g         3409 t
ORIGIN      
        1 agatggctgc tgatataatc tactgcgcca ccgactgttt caatttgttc agcgtcttca
       61 tctgaaattt ccgtaccgaa ttcatcttca agctctaaaa cgaattccat aatactaatt
      121 gaatcagctt ttaaatcatc tttgatgttc aattcatccg tcactttatc ggtatctatc
      181 tcaaaatgat ttgaaataat tttcgctact ttgttaaata cttcttcgcg agtcaacttt
      241 ttcacctcca agaatcatac acttgtcaat tacctacacc atggctaatt ttactggcat
      301 ttttcaggaa tgtccacctt tttttcaatg gaacggatcg cttcagatat ccaaatacta
      361 ttcttcgctt ttttcaaatt gttcgactaa ttgtccaact acatctgtct caagcattgt
      421 gtggatttgg cggatcgtat agcgaacagc atcggggcct gttgctccat gagttttaat
      481 gactggtgct ttcaagccaa acaaaacggc accgccatgt ttggaataat ccatttctgc
      541 tttcaaagaa cgtaagccgt cttttaaaag catagcgccc atttttcctt tgatcccttc
      601 atttagaata gcagatttta atagattcat catattcatt gctgttcctt caatagattt
      661 caaaacagcg ttgcctgtaa atccatctgt gaccactaca tcagccacac cattgagtag
      721 ctcacgagct tctacgtttc caataaaatt caagcttgtt tcattctgaa ggagttcata
      781 ggcttttttc gtcacttcac taccttttgt tgcttctgtt ccgttgttta agagtgcgac
      841 acgaggacgt ccgatacctc ggactttacg tgcataaaaa gaacctaaaa taccgtattg
      901 caacaaatgt tctggtttgt tttctgcgtt agcgccaaga tccagcatat cgaaaccggc
      961 accttctttc cctactactg gcaaggtaga catcaagcca gggcgttcga tatttttgat
     1021 acgtcctacg atgaacaacc ctgctgctag tagcgcgcct gtattccctg ctgaaaatac
     1081 agcatctgct tcaccgtttt tcactgcttg tgcagcaagg accattgatg cagtcttttt
     1141 tcgtcgaatt gcttttactg gctcatcatc actattgatt ttttcgtctg tatggataat
     1201 cgtgatgttt ttttcatttg ttaagtattt cttgatttct gcttcttttc cgtaaagtaa
     1261 gaattctact tctggaaaat cttgctgcgc taaagccacg ccttctacaa ttgcttgagg
     1321 tgcgtgatct ccgcccattg catctacggc aattttcatt gtttccactc ctttttccgt
     1381 catcagtata aagtatagca cagtctgcta atcgaaataa cgattatcct gattttctat
     1441 ttggaggctt tgcgctaaag gctgatactc aggaagcatc tgccaatcct ttatttgcca
     1501 aagggctgca gcctcttctc tggctacctc caaaacattt ccatccgtca ccaagtcagc
     1561 aatcgcaaat tgaggcagac cagattgacg gaagccaaat acttcaccag gaccacgtaa
     1621 ttccagatct ttttcgctta gtacaaaacc attggtggtc tctgtcatga ttttcatccg
     1681 ttccacacct agttcatttt taggattagc aactaagata cagtaggaag catcacttcc
     1741 gcgtccaaca cgtccacgaa gttggtgcag ctgtgccaat ccaaatcgat ccgcatccat
     1801 gatcagcatg acggtcgcgt tcggcacgtt tacccctact tcaattacag tagtcgaaac
     1861 taatatctgc atctggttgt ctttgaaggc ttccatgatt acttcttttt cctgattctt
     1921 cattttacca tgtaacaagc caacttcata ttttggtgca aatagttcac gaagtttttc
     1981 atagatttcc actgcatttt ttacatctaa tgcttctgat tcttcaatca gcggacagat
     2041 gacatacatt tgatgtcctc ttgccagttc ttttttcgtc caatccagta cactgtccaa
     2101 ttgaggtgtt cttacccagc gtgtttcgat cgggatacgt ccagctggaa gttcatcaat
     2161 gatagataca tccatctcac catatgctgt aatagctaat gttctaggaa tcggagtcgc
     2221 tgtcataaat aagacatctg gtcgccagcc tttttcacga agtactttcc gctggttcac
     2281 gccaaaacgg tgctgctcat ccgtaataac tagacctaaa ttctgaaatt cgactccatc
     2341 ttggatcaat gcatgtgtgc cgatcacgac gttaatttcc ccttgctcta gtttttcaag
     2401 gagctcacgt cgttcttttg ctttcgttga ccccgtaatc aatgcgatgg ttacttcatt
     2461 gggatcaaac agctgttgca agctttccat atgttgttgt gccaagattt ctgtaggcac
     2521 catcaacgca ccttggaaac ctgctgtaac ggtagcgtat aacgcaatcg cggcaaccac
     2581 tgtttttccg ctacctacat ctccctgcaa aagtcgctgc atatgctgcg gactgcgaag
     2641 atcccgacag atttcattcg ttacacgttt ttgtgcattt gtcagttcaa aaggtagctt
     2701 ttgagtaaat tgtttcaatt tttggacatc ataaaggatc tccaaaccat tcgtttctgc
     2761 tttttctgct tttttcaatc cttgaagacg catttgaaat aaaaagaact cttcaaaaac
     2821 gacacgtcgt ttggcttgat ggttttcttc gtggtctttt ggaaaatgca tacttcttac
     2881 agcagaagct cggtcaagca gtcgatattt ctccacaagg ttgtttggca gattttcttc
     2941 aatcaatgtg ccgtattctt gaaacccttt tttgattaga tcaactaatg tcgtttgacg
     3001 aattgatttg ttgacgtgat atatcggcga aaagtcatcc attgtttgag aacctaaaat
     3061 cttcatcccg ttcaaggctt tacgctttgc atcccattta ccgtatacgg cgatttcctc
     3121 cgataaaatg actttttctt ttagataagg ttgattaaaa aaagaaacgt tgaacacatc
     3181 atgttcttgc atcatacgaa attgcaatcg gcttttttta taaccaaatc gattcatcac
     3241 aggaggcgag actacgattc ctttcaaagt aaccttttct tgatcctgga tctcgtttag
     3301 atttctttcc tgaatatctt cgtagcgaaa aggataataa gtcagtaagt cttcaatcgt
     3361 ttggatgccc aagctggcaa gactagctgc tcttttttct ccgactcctg ccagcatagt
     3421 gattggatca tctaaagaac gcataaattt ttcctccctc ctttaacaaa aaaaggactt
     3481 ggtcagcgat catacctgat cactaaccga aggcctttta tgaatgaata ctgtgttaga
     3541 aagatattct gacacagaaa ttattcagca gaaaatagat aaggatagac aggctgatct
     3601 ccttggtgga tctcgatttc caaatcagga tgtttttctg ttagtttctc agcaaattca
     3661 tttgcttgtt caactgttcc ttcatcacct gccaagatcg ttacgatctc tgtgtcctca
     3721 ttgatcattt gttctagtgt acttaacgct gttgctgtga tatctgggtt agagatcagg
     3781 atttttccat cgatcatgcc caaaaagtct ccttctttga tctctacacc gtcaattgag
     3841 gtatctctga tcgcgtgagt gacttgaccg ctcacaacgc tagacaacat ttctgtcatc
     3901 gtttctttgt tttcttctaa agaagcttga tcattgaatg aaagcatagc tgtcaatcct
     3961 tgggaaatcg ttcttgaagg aacaactgct actgggatat ctgctacttc tgctgcttga
     4021 tcagctgcca tgaagatgtt cttattgttt ggcaagacaa tcactttatc tgcgtttact
     4081 tcttgaatcg ctttaacaat gtcttccgta cttggattca ttgtttgtcc accactgata
     4141 acataattag cgcctaaact ttcaaacagc tcttggacgc ctttaccagc agcgatcgct
     4201 acgataccat acgggatacg tggtatttct tttttcgcaa actcgttcac ttcttcatcg
     4261 tgttccaaaa tcgtctcatg ctgcaagcgc atgttgtcga ctttgatttt gactaatgaa
     4321 ccgaattttt gtccataatt caagacttct cctgggtttt ctgtatgcac gtggactttg
     4381 atgatctcgt catcattgac cacaagtaaa gaatctccta taccatctaa atagttacgg
     4441 aattcttcgt aatcgaatgt actatcgact gtaggtcctt ctccgatttt taccatgatt
     4501 tctgtacagt aaccaaaatg gatatcttct gtcgccattt gaccttggac actgcgatga
     4561 tgttctgcat tgaccatatc atccatttct gctggagaag gttcatatgt ttcatccgct
     4621 tggaattctc cattcaatgc acttaaaaag ccctcataaa caaacagcag tccttgccct
     4681 ccactgtcta cgacaccgac ttcttttagg actggaagca agtctggagt tttagctaaa
     4741 gcacgctttg ctccttttac aacagctgtc attacttcaa cgacatcatc agtacttttt
     4801 gctttctttt ctccagcttt cgctgcttct ctagctactg tcaaaatcgt tccttctact
     4861 ggtttcatta ctgctttata cgcagtttca actccatgag tgaaggctgc agcaagatca
     4921 gaagcattta atgagacaac atctggaatt tgcttagaaa atccacgaaa caattgagac
     4981 aaaataacgc cggaatttcc gcgcgcaccc atcagcaatc cttttgaaag cattgctgct
     5041 aattctccta ctttttctga ggttgaatct actactgcct tagctccact agtcatagat
     5101 agattcatat ttgttcccgt atcgccatct ggcacaggga atacatttaa tgaattgacg
     5161 tattctgcat ttacttgcag acgattcgca cctgcctgga ccatttcctg gaactggctt
     5221 gcacttattt ctgttacact cacctagata atcctccttc agatcgactg ctgagttacc
     5281 agccagtctc ctgcctagtc tggcagtaca cgaacacctt gtacaaagac attaactgaa
     5341 ttggcagtta cgcctaataa tgcctctaga ttgtatttca ctttttcttg gacgttgcgg
     5401 gaaacttcag agattttcgt accatagctg ataatcgtat aaacatctac agcaatacca
     5461 ttatcttctt ggcggacaac tactcctctt gagtaatttt ctttacgcaa aatctcagta
     5521 atattatctt taatttgatt tttgctggcc attcctacaa caccgaacac atctgtcgcg
     5581 gcgccaccaa caactgttgc aattacatca ttggtaattt caatggttcc agctgacgtt
     5641 tgaattttta cagccatgaa tgagcctcct taacgggtaa aaaactccca tttttcttct
     5701 atcatatttt atcatagcgc aacttgaaat aaaaggcagt tacccaaagt actcaaaaac
     5761 cttgcaaaat ttcaggtata accttgttta atttacaatt attcttctca attgtcaatt
     5821 gtatctatga aaatgtactt gcaaattccc attctatatg atatgttagt ctggtatgag
     5881 ctgaattatt ctattgctcc aaatcaaggc aaaggaggaa attaccatgg caaaagtttg
     5941 ttactttaca ggtcgcaaga caaaaagtgg aaacaaccgt tcacacgcta tgaactcaac
     6001 aaaacgtact gttaaaccta acttacaaaa agttcgcgtt atggtcgacg gaaaacctaa
     6061 aaaagtttgg gtgtcaactc gtgctttgaa atcaggaaaa gttgaacgcg tttaattgaa
     6121 tgatacttgg accgttcaga tagtgaacgg ttttttattt aataaaaatt tttaagggtg
     6181 ggacttttga ttcctaccct ttttttgcgt ttatttttgt tatacgaaca aattatgaag
     6241 ttatttattc taaattttat ttttttgtga aaactagact ctttttaagc agagaaaaac
     6301 aaggatatga taaactgaat acaacttcat agaaatttgg tggaaaataa atatgaaaaa
     6361 aaatttaaag tcattcttta gagaaaacgg acgctacgca gcagctagtt tcctgattcc
     6421 gtttttgatt ttagcacttg tttatctaag tatcggaatc tatcctggaa gcagcagaag
     6481 tattttggca agtgatgcct tttcacagtt ttctaatttt catgccagct tccgaaatgt
     6541 tcttcttgga aagcaaagta ttttttatac atggaatgct tctctaggat tgaattattt
     6601 atctttaatt tcttactatc taggcgggat cttcacgcca ctggtgttct tttttccaaa
     6661 ccagatgatg ccagatgcac tgtatgttct gactttgctt aaggtcggct gtgccggatt
     6721 ggctttttgg tttcttgcac gtacgttcaa gattcctcgc tgggggcatg ttgcacttag
     6781 cgtcagttac gcattgatct catttatcac tgcacattcg gagatcatca tgtggctgga
     6841 tgcatttttt tatcttccat tagtgatttt ggggattcat cgtgttatgg atcttaaacg
     6901 gccgaaactt ttatttatca gttatttact cttgttttta tccagttttt atatgggttt
     6961 tatgattggc gtattttctt tcttatattt tatcgcccgt ctattgacaa actggtcaga
     7021 atataaaaaa acgattgttc cttatggtat cacttcttta ctggcaggcg gcgcttcaat
     7081 gatcattgta ttgccagcag tgctggatct tcggtcaaat ggcgaggcac tgagtcagat
     7141 cactacgttg aaaacagaag cgacttctta tctggatcta atcatgaaaa atatgattgg
     7201 tgtttatgac acgacaaaat acggatcgat tccgttcgtt tacgcaggtc tgctaccatt
     7261 agtcctctgt ctgttttatt ttgccagtcg gaaagtaccg ttgaaaaata aatttttgta
     7321 cggcagtctt ttcgccatac ttatcgcaag tttttatctt gttccattga atttgttctg
     7381 gcatggtatg catgcaccga atatgttttt attccgttat gcctatctgt tctctttctt
     7441 agtgctcctg cttgcaggaa aaggatggga aaagatagaa caagacgatc gtgggctgtt
     7501 gttaggaatc attttggtcg tagctgctct ctttgcggtg gcatggggca tcaaacctga
     7561 aggaagctac acctatgtga cgatgacttc ctttgtattg acagtggttt tcttagggat
     7621 ttatgctttt gctgtgggat tttatcagca taaaaaactt tcattcaatc atctgagtat
     7681 attgttatta ttgatcatgt ctgctgaagc actggtcaat acgaacagta tgatccatgg
     7741 cattcttgat gattggaatt atgcttcccg cagtttatat actgatcctt atcctgactt
     7801 gaaagctctt gtaaatgaaa caaaaaaaga ttccgactcg ttttaccgct tggaaaatct
     7861 ggatccagta tctgcaaacg attcgatcaa ctatggttac agtggtatca gtttgttttc
     7921 ttctataaga aatcgtaatt cttcctctta cctggataaa ttaggtttcc gttcaagagg
     7981 gacaaatttg aatatccgtt ataacaataa tactttgctg atggatgggt ttacaggaat
     8041 caaatataac atttctaaaa acgaaagcac gttcagtaaa tatggcttcc gccaagctgg
     8101 tcaaagcggc gactatacac tgtatcagaa tgtcaatgca ttgccactag catttactgc
     8161 accgttgtcc gttaatgatg ttgagcagcc agtgacagat aatttgacca gtcagacaaa
     8221 cttgatcaat acattgtctg gtctaaatga acagtatttt acattctata cacctacttt
     8281 gaagaatcag ttgaatacga cgatcacaaa gacaacgact ggcgtcactt actcagaaac
     8341 agcagataac cagccaaaag agctcacttg ggaagtccat gtgcctgcaa atacacaagc
     8401 ttacttaagt ttgttcccaa ccaattttgc gcaattagaa agttctagtg cgacgatcac
     8461 tgtcaatggt acatcgcgaa aaacgcaaat caacatttct ggtcaatact atgatcttgg
     8521 ctattatcct caagagacaa cagtgacatt tactgcaagt ttttatggga cgaaagaaat
     8581 aagtttcatg gagcctaaag tcgttggtct agatgttgta gcctatcaag ccgcaatgaa
     8641 tcagatcaaa gaaaaaggtg tcgaaatgga agccactggc agaaaagctt ctgggactgt
     8701 aaataccgct gaagattcca tgttggtgac aactattcct tatgacaaag gatggaaagc
     8761 taaaattgat gggaaaccag tcaaggtcag caatttcaaa gacgcattct tgatgataaa
     8821 agttccagca ggtaaacata ctgtcacttt cagttacttg cctgaaggat ttacgatagg
     8881 tgcaatcctt tttgtcctat gtattgcatt gtttattctt tatgtctggt accttgaccg
     8941 caatcgcccc ttttatggca cgattgatcc agcaatagaa gaacaaccac gaaacacaca
     9001 cagaagaaga cgtagaaaat aaagaaacga tggaacctgt tcagactgtc cgcaccaagg
     9061 gcaatcaaaa caacgttcca tcgttttttg gttacagaaa aaataggtaa caaaaagatg
     9121 attcacttat caacgcctcg tccacaagtc acgatattca tcaaagatga gaagctgttt
     9181 tttgaaattg ctctttgtga ctcagagtta aatgacaggc tcacgacctc tttcttaaac
     9241 ggtgttcaat cagctgacct tcggtattag ctcaacaaac ggcaaaatat gaggagctat
     9301 tttcccgttt gtttctctaa tactcaggtc aaaacgctga tttcacgacc tcttccttaa
     9361 acggtgttcc agaagtagct tcttcgaaaa taagccgaaa ttttgcaaaa ttttgagaag
     9421 taattttcgt aaataccttc ttatttcttg aagctgacca cttctatcat aacctcttat
     9481 tcatatttca cttcatacaa ataaagtcct tcagggtgtg ctgtaggtcc tgctgcatta
     9541 cgatcttttt tcgcaataat atctgggata ctatcttcag gcaatcggcc attgccaatt
     9601 ttcaaaagcg ttcctactaa gatccgtatc atcttataca agaagccatt tcccctaaat
     9661 gtaaacaaca attcgtcttt tgtttcattt acctccatct tagcctcgta gatcgtacgt
     9721 attttatcct ctatcgagct tcctgatgca caaaaagagg tgaaatcgtg agtccctaat
     9781 aaatcaggaa gcgcccgctg aattttttcc aaatcaagtg ggtaaggaaa ataacttgca
     9841 taatggcgtc gaaaaggact gcgtggtttc cctatatcca ctcgaaattg atacgtcttt
     9901 tctttgacca aatatcttgc gtggaagtct tctgaaacaa tttctacttg tcttacagca
     9961 atatcctctg gtgactgggt atctagcgcg aaacgcattc gttctaacgg acgctcctct
    10021 ggataatcaa aatggattac ttgcccgatc gcatgtactc cggcatctgt ccttcccgaa
    10081 ccaaagaccg tgatttcttt tccatttgcc attttttgta atgttttttc tacttcttct
    10141 tgaacagtac gcccatttgg ctgtttttgg aatccattaa aatttgttcc gtcgtacgca
    10201 atgattgctt tgtaacgtac catgtttttc tctcctttgt tcctaaaagg ttatctggaa
    10261 taatgatttt tctataa
//
LOCUS       DAZKNH010000090        10012 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000090 DAZKNH010000000
VERSION     DAZKNH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.090"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..366)
                     /locus_tag="ACG8MG_002152"
     CDS             complement(46..366)
                     /locus_tag="ACG8MG_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0376170.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(514..2748)
                     /locus_tag="ACG8MG_002153"
     CDS             complement(514..2748)
                     /locus_tag="ACG8MG_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0376171.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3227..3493
                     /locus_tag="ACG8MG_002154"
     CDS             3227..3493
                     /locus_tag="ACG8MG_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFQ0376172.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3493..5220
                     /gene="ptsP"
                     /locus_tag="ACG8MG_002155"
     CDS             3493..5220
                     /gene="ptsP"
                     /locus_tag="ACG8MG_002155"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFQ0376173.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5384..6430
                     /locus_tag="ACG8MG_002156"
     CDS             5384..6430
                     /locus_tag="ACG8MG_002156"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0376174.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6447..7670
                     /locus_tag="ACG8MG_002157"
     CDS             6447..7670
                     /locus_tag="ACG8MG_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0376175.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7759..8646
                     /locus_tag="ACG8MG_002158"
     CDS             7759..8646
                     /locus_tag="ACG8MG_002158"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0376176.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8732..8962
                     /locus_tag="ACG8MG_002159"
     CDS             8732..8962
                     /locus_tag="ACG8MG_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFQ0376177.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(9019..9984)
                     /locus_tag="ACG8MG_002160"
     CDS             complement(9019..9984)
                     /locus_tag="ACG8MG_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0376178.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3180 a         1776 c         2026 g         3030 t
ORIGIN      
        1 tttacttttg tcccacgctc tttaattatt atgttcaaaa tttgtttaca cggctaatgt
       61 agcgcctaat tcatgttttg gaatcgagat ttcaacaagg cctgccaatt cgattacttt
      121 gatttcatcc tcgtggtaaa cggcgtcttt gattttatct aagaaataat ttttagcatc
      181 ttcgatttct ttcggctgga ttttacgttt gtcattgatc agcagaattt cattatgttg
      241 aggggcttca gtgtagacaa tagtggtatt tccggctgtg tagaccttga ctagatgggc
      301 atcagtgttt tcaagctggt ttaatacaag acgggaatgg ctgtttgtta catttaccaa
      361 tttcatggcg ctcacctctt ttatgtattt ttaattgctt gtttctctta ttataatttc
      421 ttttttataa aatgaacagt attttgactt ttctgaaaaa agaatgagac ggcatttctc
      481 aaagaaatac cgtctcacgc tataaaactg gatttattca gccgtttctt cggctgattc
      541 tttagccaaa cgttctggtt tagaagtaac gatgattttt ccatctttat ccagttttgc
      601 tttcaactca tggatagaag ggtgatcaag ataaaattca gcgataccat cttcgatttg
      661 ttcttggatc gtgcgtcgaa gaggacgagc acccatagat ggatcatagc ctaagtcaac
      721 taatttttct ttgacattcg taggaacttc gatgtgaagt tgctgtgctg ccagcatttg
      781 gttgacatcg tctagcatta gtgtgacgat tgtcatcaag ttttcttttg ataatgcact
      841 gaattcgatg atcccgtcaa aacggttcag aaattctggt gtaaagtagt tgtttaattg
      901 gttcaataca gaacgagtaa caccttcgcg tgccgcacca aaaccaacgt ttgcttctac
      961 tttaccagtt ccggcattac ttgtcataat gatgatcgta tctttaaagc tgaccgttct
     1021 tccttgtgca tctgtcaatc gaccatcatc taagatttgc aagaacatat gcaaaacatc
     1081 tggatgagct ttttctactt catctaacaa gacaagactg taaggatttc tccgtacttt
     1141 ttctgttaat tgaccagctt cttcgtatcc gacataacct ggaggagaac caatcaattt
     1201 agagacacta tgtttttcca tatattcaga catatcgaaa cgaatcattg aatcttgtga
     1261 accgaatagt tcgtaggcaa gttgtttcgc aagctctgtt ttacctacac cagttggtcc
     1321 aacaaataag aaggagccga ttggacgatt ctgtttgttt aatccaacac ggtttcggcg
     1381 aatcgctttc gctactcgat ctacagcgtt atcttgtccg atgacatgag ctttcaagtc
     1441 atctgctaag tttttcagtt gtgtctgttc tttttctttt aattcaccca ctggaatacc
     1501 tgtccgttgt tcaacgattt tttccatatc tttttcggta atcacaggtg tttcctcttc
     1561 agtgagctga cgttctttca ttttttgaag tttattgatc tgatcgcgat aataagctgc
     1621 tttttcgaaa tcttcttctc gagaagctaa aactttttgt tcttctgctt cttgcagttt
     1681 tttctcgatt gttttaggat caacgatttg gatcgtcaag tttttcttag aacctgtttc
     1741 atctagcaaa tcgatggctt tatccggcaa gaagcgatct tggacgtaac gattggataa
     1801 agttgcggct gcttcgatgg cctcatcagt atattttaca tgatgatagt cttcataacg
     1861 tttttgcaaa cctttcaaaa tactgatcgt ttcttcgaca gttggttcat ctacccgtac
     1921 aggttgcata cgacgttcta aagcagcgtc tttttcgatg atgcggtact catttaaggt
     1981 agtagcacca accatttgca attctcctcg agctaaagct ggttttaaaa tattaccagc
     2041 atccatattt ccatcaccag cagaaccagc accgactatt tcgtgaactt catctatgaa
     2101 taggatcaca ttttctgctt gacggatctc ttcaatcaat ttttgcatac gttcttcaaa
     2161 ttgtcctcgt ataccagtac cttggactaa agatacgaca tctaaacgaa tgacttcttt
     2221 gtccattaat ttttgcggta catcaccatc tacgattttt tgtgctaaac cttcaacaac
     2281 tgctgtttta ccaacacctg gttctccaat caagacagga ttgttcttgg ttcggcgatt
     2341 taaaatttca atgactcgtt tgatttcgtc atcgcgtccg atcacaggat cgatatcacc
     2401 ttgtcgtgct tgttctgtga tattgattcc gtattctccc aataaacctt ctgtattgcc
     2461 ttgaggtggc tgaccaccat tgaagttgtt gtttccgcca aattgtgttg gtggtgtttg
     2521 ctcataattt ccttgctgtt gcatttggcg agacatggaa cggaaaagat cgtccaaact
     2581 gccaaatcca aatggatcat tttgggccat attagtcaga cctccatttt gttgattttt
     2641 gattttttga taacagctct gacagtaatc gagttgtgtc cgctgaccat tgacagtggc
     2701 atacaagtga atcgttgcct cattcttgcc gcagttttga caaagcatga atcattcacg
     2761 tcctttcaca aaaatgggta catccatatt ttacatctcg tttttcatga ggtaaaagaa
     2821 taacccctag ttttttaaga gatttatgag gtagaaaagg tgaaatctct attgaccatt
     2881 cctgaccttt gactcttatt atactaaaaa taaaaaggga agcaagtaat tcgtttcata
     2941 tgaaaacaaa gagggtcaaa ggaaagaatg ataaacgtca ggagaagaga aagcgacaag
     3001 aaaaaggaaa taaacacgtt tgttataaaa tgtgaagaaa aggatgaaaa agaatccgga
     3061 ataatttgaa tacttttatc tgaaattgaa tgaaaactgt tgtatctaac gaaaaaaaat
     3121 gttaatcttt acagatgaaa gggtggcatt tgttaggatt ctctgaatta ctaaaaaaga
     3181 aaaccctcac ataaaaacaa cactcactta aaggagaccg attaatatgg aaaagaaaga
     3241 atttcacgta gtagcagaaa ctgggatcca cgctcgtcca gctacactat tagtacaaac
     3301 agcaagcaaa tttaactctg atgtaaactt agagtataaa ggtaaatcag taaacttgaa
     3361 atcaatcatg ggcgttatgt ctttaggtgt aggtcaaggt tctgatgtta ctattactgc
     3421 agaaggtgct gacgaagctg atgcaatggc agcaatcgtt gaaacaatga agaaagaagg
     3481 cttatctgaa taatggttga aatgctaaaa gggattgccg ctagtgacgg tgtagccgtt
     3541 gcaaaagctt acctgctagt tcaaccggat ttaacattca gtaaggcaac agtagaagat
     3601 actgcggctg aagaagctcg tttggatgct gcattagcta aatctactga agaattgcag
     3661 caaattcgtg aaaaagcagc gcaaagctta ggtgaagcag aagcgcaagt atttgatgcc
     3721 catttgatgg ttctttcaga tccagaaatg gtaggtcaaa tcaagcaaaa tattaaagac
     3781 aacagcgtaa atgctgaatc agcgctaaaa gaagtaacgg atatgtatat cggtatgttc
     3841 gaagcaatgg aagataatgc ttatatgcaa gagcgtgctg cagatattcg tgacgttgct
     3901 aaacgtattt tagcgcactt attaggcgtt actttaccaa atccatcaat gatcaacgaa
     3961 gaagtggtcg ttgttgcaca tgacttgact ccaagtgata cagcacaatt agatcgcaat
     4021 tttgtaaaag cgtttgtaac agacattggt ggacgtactt cacattctgc tatcatggca
     4081 cgttctttag aaattccagc aatcgttggg acaaaagaaa ttactgctaa agtcaaagaa
     4141 ggcgatattt tggctgtcaa cgggatcgaa ggcgatgtaa tcatcgatcc tacagatgaa
     4201 caaaaagctg aatttgaaaa agcaggtgca gattacgcag cacaaaaagc agaatgggaa
     4261 aaattaaaaa atgcagaaac tgtcacagct gacggcaagc attttgaatt agctgcaaac
     4321 atcggaacac caaaagatct agttggtgtc cataataatg gtggagaagc agttggtctg
     4381 taccgtactg aattcctgta tatggattct ccagacttcc caacagaaga tgaccaatat
     4441 gaagcttaca aagctgtatt agaaggtatg gaaggaaaac ctgtagttgt tcgtacaatg
     4501 gacatcggtg gagataaaga actaccatat ctgcaattac ctcatgaaat gaatccattc
     4561 ttaggatatc gtgcattgcg tatcagttta tctgaacaag gcgacgaaat gttccgtaca
     4621 caaatgcgtg cattgcttcg tgcatctgtt catggtaatt tacgtatcat gttccctatg
     4681 gtagctacac tcaaagaatt ccgcgcagca aaagcgatct ttgaagaaga aaaacaaaaa
     4741 ttgataagtg aaggcaagga agtatctgat acgatccaag tcggcatcat gatcgagatt
     4801 ccagcagcag cagtcttagc agacaagttt gcaaaagaag tagatttctt ctctgtaggt
     4861 acgaacgatt tgattcaata cacaatggca gcagaccgta tgaacgaacg cgtttcttac
     4921 ttgtaccaac catataaccc atcaatttta cgtttgatca aaaatgtaat tgatgcagca
     4981 cacgctgaag gaaaatgggc aggtatgtgt ggagaaatgg ctggagatca aacagctgtt
     5041 ccattgctag taggtatggg tctagatgag ttctcaatga gtgcgacatc tattcttaaa
     5101 acgcgtagtt taatgaaacg cttggacaca gcgaaaatgg ctgaacttgc tgatcgtgca
     5161 ttgaaagaat gcgatacgat ggaagaagtc gttgaacttg ttcatgaata tgtaaaataa
     5221 acaacgtatc aaaagctggt gtggcttgta aaagagctac gccagttttt ttgttctttt
     5281 ttaagaaaac gtttttgtca gtaagaatag acaaaaatag ggcttaagtt tgagtgacag
     5341 tttgtaggct atgttatact atataagtga agggggacgt aaggtgaagg tattattata
     5401 ttttgaaagt gaaaagatgc ttgcaaaatc tggaatcggg cgtgcgcttg atcatcaaaa
     5461 acgtgctctg acggaagtcg gtatctctta tacactagat gaaaaagaag attatgacat
     5521 cctgcacatc aatacatacg gaatcaatag tcataatatg gtcaataaag ccagaagaga
     5581 aggaaaaaaa gttgtctatc atgcgcattc tacagaggaa gatttccgaa attcctttat
     5641 cggatcgaac cagctttctc ctatcgtaaa aaaatatctg gtaggattgt accaaaaagc
     5701 ggactatctg atcacgccaa caccttattc aaaacagttg ttggaaagct atggtatccg
     5761 tgtgccgatt caagctattt ccaatgggat cgacttagag aaatatcacc ctgatccaat
     5821 gaaagaacaa aagttcagag aatatttcaa actgtctccg gatcaaaaag taatcatttg
     5881 tgtcggtttg tttttcgaac gtaaaggaat cattgatttc gtagaaattg ctaagaagat
     5941 gcccgaatac acatttattt ggtttggtca tgttcccatg tactcgattc ctcggaacat
     6001 ccggaaaatt gtaaaagaag atcatcctga aaatgtcctg tttcctggtt acattcgtgg
     6061 agagatcata gaaggtgcgt attcaggtgc agatctattc tttttccctt cttatgaaga
     6121 gacagaaggg atcgttgttt tagaagcttt agcaagcaaa caaaacgtac ttgttcgaga
     6181 tattccagtg tataatggct ggttagaaga tagcaagaac tgctatatgg ggaagacaaa
     6241 cgaagaattt atccgactga tccaacagat taccaatcaa gaactaccga gtacgacaca
     6301 agccggttat caaacagcaa aagagcgaag tatcaataaa atcggtgaag aacttaaaaa
     6361 tgtatacgaa agtgtactga atgaaaaagt ctctagcaaa ttaaaaaaag taaatcaggt
     6421 caaggactag tttggaggcg aaaaacatga aaatcggctt ttttaccgat acctactttc
     6481 cgcaagtcag cggagttgct acatcgatca aaacattaaa acaagaatta gaaagacatg
     6541 ggcatgaagt ctatattttt acaacgaccg atcctaatgc ggataagttg gaaaaagatg
     6601 tgattcgaat gccaagtgta ccatttattt ctttcaagga tcgaagagtt gttgttagag
     6661 gcatgtggta tgcttatttg gttgccaaag aactagaact agatcttatt catacccata
     6721 cagaattcgg agcagggata cttgggaaga tggtagctaa gaagttgaaa atacctctga
     6781 tccatactta ccatacgatg tatgaagact accttcatta tatcggtaaa ggaaaagtga
     6841 tcaggcaatc ccatgtaaaa tacttgtcta gattatttgc aaatcatacg acaggtgtag
     6901 tttgtccaag tgaacgtgtt attgatacat tacgcagcta tggtgtaata gcaccgcttc
     6961 gcgtgattcc tactggtatc gacattgaaa aattcaaacg ttctgatatc actcagcagg
     7021 atatcagcaa attaagggag tcactaggtc ttcaggaaaa ccatttgatg attctttctt
     7081 taagcaggat ttcttatgag aaaaatatcc aagccttgat taatgggttt cctcaggtca
     7141 tctccgctta tcctaacgcc agattagtta tcgtaggaaa aggaccttat gtagaagagt
     7201 tagaggaatt gatctcagaa ctgcagttag aagaatttgt gcagttcaca ggagaagtgg
     7261 acaacaatga tgtagcactt tattataaag cggcagatta ttttgtcagt gcttctactt
     7321 cagaaacaca agggcttact tatacagaag cgatggcagc cggaacacct tgtgtcgtgg
     7381 aaggaaatgc ttatttgaac cgattgttcg atcatccttc attagggaaa acttttgaaa
     7441 ccgacgaaga ctttgcacca gcacttattg attatatttc cagtgaagtt cctaaagatc
     7501 caaaagtttt agaagctaaa ctttatgata tttcatctac acatttcggc gaagctatga
     7561 tcgaatttta tcaagacatg gttgtttatc atgaagaaga gatgcgaaga aaagaagaag
     7621 aagactcgat tgaacgaatc aaaataaaat taaattcatt taaaagataa aattcaaaca
     7681 ttttttatga aaaaatcctt gtttctagcg tatactagca tagaagcaag gatttttgat
     7741 ttttaggagg tcgaaaaaat gaaacttggt tttatcggaa caggcgtcat gggaagtgct
     7801 gtcgcacgcc atctactaga agcaggacac gaagtagctg tctataatcg gacaaaagca
     7861 aaagctgatc ctttagtaac agaaggagct atctgggcag atacaccaaa agcggtagca
     7921 gaacaaagta atattctctt tacaatggta ggttacccca aagatgtaga agaaatctac
     7981 tatggacagt cggggatttt ttctgcagat atctcaggac atattttagt tgatttgact
     8041 acaagtacac caagcctggc agaaaaaatt gcaaaaacag caaaagaaaa aggcgcagat
     8101 gcgttagatg caccggtatc cggtggtgac ttaggagcaa agaatggtac actgacgatc
     8161 atggtcggtg gagaagaagc cgtttatgat caagtactgc ctttattcaa agaatttggt
     8221 acgacattta cattgcatgg ttctgctggg aaaggtcagc atacaaaaat ggctaatcag
     8281 atcatgattg caggtacgat gacaggaatg acggaaatgc ttgtctatgc acaaaaaaac
     8341 ggacttgatt taaagaaagt gattgagacg ctttcaggtg gaagtgcagc taactggtct
     8401 ttaagcaatt attctcctcg tattttaaaa gaagattaca cacctggttt ttttgtcaaa
     8461 cattttatca aagatttaaa gattgcatta gaagaagcag aaaaaatgga tcttgtattg
     8521 ccagcaacta cccaagcatt gaaactatat gaagagttgg cagataaagg ttttgagaat
     8581 gacggaaccc aagctttgat caaactttgg tggcctgaag gaaaaatacc agaaaaaaaa
     8641 tcataaaaaa cgagtttttt tagagaaaat caatgcaagt aggctatgct tttgatacaa
     8701 tataacagaa ataagaaaag gaggacctcc gatgacacat tcacaattag tcgcaatcat
     8761 aaaacgactg gaggctatgg tcgagtcagc agataatgaa ctgcaagttc gccgttttga
     8821 aaaagaaggc gtagagaagt gtacagtaac ttatgataaa gcgaccgata catttgaatt
     8881 aactgaaaca gatactcatc agcaatacga atttgataat atcgatatcg tagcgatgga
     8941 aatctatgac ttaatccaat aaattttagg ataattgaca tttgaagttg aagatgccta
     9001 aaagaagccg agaagcaact aatttagttg cttttcagct tcttttttgt ttaacagttc
     9061 aatttcaaac tgttcttttt ctaatttttt gcggtaactg atcaagattt gtacaggatc
     9121 gatttcttct tttgacaatt gacgtttcaa agataaatga gcttctttct tttcataata
     9181 gaagtcagga gaaacgtcac cagaggtaat cattgtttct gcaagttgac cataacaaac
     9241 gaggtagtgc tttttctgac tctcatctgc tgctaatggc atgctgaagg tgagcgatgg
     9301 tgttcttaaa aactgctttt gttcctggat ccgtctaata aaataagtaa ctaattcgat
     9361 atctttttga agcataccgg ctttttcgaa attcaactga tagatgaaat gttgttgtct
     9421 ttttttcaag taagtcagta gctttttatt tcggccttga aacagccctt ggagttcctt
     9481 ttttttctgt tcaaaagtca tttcctgtag gtcaggaagc tggactcgca aagctaacaa
     9541 actaatttca gacaaccaag gcatttgata agtttcttcc attatacgca aaatagacgg
     9601 cattttttta tagagacgaa aaggcccgta tgttcgagcc gtaggagtat ctgttaatac
     9661 aaatccctta ttggtaatat gtacataatt ataattactg aaataattca tttgtcggtt
     9721 ataaaaagga cggtaatgtt ggatcaattg acactcgagc aataaagcat ctaattccgt
     9781 gtctactacg acgaaatcaa aatctgttat atgatgaatc atacgtaata ctttttttga
     9841 atgctgttta ttctgatgaa aatagctgct tactctattt ttgagccgtt ttgctttacc
     9901 aacatagatg accttaccat ctcggtcttt cataagatag acacccggca gcatggggag
     9961 agttttcatt ttttctttta acataggatc tccttttacg ctttgaataa aa
//
LOCUS       DAZKNH010000091        10005 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000091 DAZKNH010000000
VERSION     DAZKNH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.091"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            188..3415
                     /gene="fss3"
                     /locus_tag="ACG8MG_002161"
     CDS             188..3415
                     /gene="fss3"
                     /locus_tag="ACG8MG_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0376179.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3528..3842
                     /locus_tag="ACG8MG_002162"
     CDS             3528..3842
                     /locus_tag="ACG8MG_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0376180.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3855..4229
                     /locus_tag="ACG8MG_002163"
     CDS             3855..4229
                     /locus_tag="ACG8MG_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0376181.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4239..4574
                     /locus_tag="ACG8MG_002164"
     CDS             4239..4574
                     /locus_tag="ACG8MG_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFQ0376182.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4657..5997
                     /locus_tag="ACG8MG_002165"
     CDS             4657..5997
                     /locus_tag="ACG8MG_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0376183.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            6075..6749
                     /locus_tag="ACG8MG_002166"
     CDS             6075..6749
                     /locus_tag="ACG8MG_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0376184.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6742..6906
                     /locus_tag="ACG8MG_002167"
     CDS             6742..6906
                     /locus_tag="ACG8MG_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376185.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            6982..8124
                     /gene="dcm"
                     /locus_tag="ACG8MG_002168"
                     /pseudo
     CDS             6982..8124
                     /gene="dcm"
                     /locus_tag="ACG8MG_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            8114..8404
                     /locus_tag="ACG8MG_002169"
     CDS             8114..8404
                     /locus_tag="ACG8MG_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376186.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8663..9847
                     /gene="mobT"
                     /locus_tag="ACG8MG_002170"
     CDS             8663..9847
                     /gene="mobT"
                     /locus_tag="ACG8MG_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0376187.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9844..9981
                     /locus_tag="ACG8MG_002171"
     CDS             9844..9981
                     /locus_tag="ACG8MG_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0376188.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         3309 a         1683 c         2257 g         2756 t
ORIGIN      
        1 tgtttgtgtt ctaaaatgtg ttctaattta aaggcagtat atttcgtatt ttagattaaa
       61 tttttaggct cattcattct caaatccatt ctattcagtc aataaaaatt ttaaaaagac
      121 tttataaaca aagatgttca agcgagcatc ttattttttt gtgcttaatt tgaaaggaga
      181 acatttcatg tataaaaaac aaaaaaagaa attatgtgta ttaactattt tgagtttggc
      241 tgttcaagca attgttggag caattgctcc tacgattact tttgctgatg aaattaccca
      301 tccacaaacg gtaaccgtag aactagattt agcacatcaa tatgctgttg aaggaacttt
      361 tagtgatggt cgtccaatgt cagaagtcac tgttccgcat tatgcagtgt acaacggctt
      421 taaacaagat attttctgta ttgaaccagg agtcccgatt tacaatgagt tcactcctgg
      481 ctatgagaaa aacccgttac cagatatgtc cgataaagcc aaattagttt ccgttttatg
      541 gaaaaaagta ggtacagatg cagatactca tattgtggca cagaagatga tctggcaaga
      601 agttaatggg tatacccttc actcaataaa acgatcagat ggtagtgccg taaatatcgc
      661 agccattgag gcaaaaataa atcaagccat tgacgattat cagaagaaac caaccttcca
      721 taatagtacg gctaaaacag tattagggca atcgactact gtgacggata cgaataatct
      781 gaatttgtca gagttcgatg aggttgtgga aaatgccgca aacattgatt atcgtgtgaa
      841 tggtaatcag ttgataatca ccccaaatgc caattcaaaa gaaagtggtg tgctgacgct
      901 taagaaatct gctggtaccg gaacacctgt cgcctataaa aaggctggcc aacagacttt
      961 aatggccggg gcgattgata aacctaatac ctacacaatc aaaattgatg tggaaacgga
     1021 gggttcttta aagatcaaaa aagtcgataa agaatcgggt gatgttgtac caggaacggt
     1081 tttccattta gactttggaa aaactttacc tgcaaaagac gtgactactg acaaagaagg
     1141 cattgctaca ttggatgaga ttcctcatgg tacaaaagta accattaccg aaaaatcagt
     1201 accggcgcct tatacgattg ataccacccc catgactacc accattaaag caggcgaaac
     1261 catttacgtg acttcaaaaa acgcacggga aaaagggcaa atcattctgg ataaaagcgg
     1321 agttgaaaca gggagtgatc tttggaatga caattattcc ttagctggta atacgtttgc
     1381 cattcgcaaa gacagtccaa ccggtgaaat cgttcaagaa atgaccacgg atgaaaacgg
     1441 tcatgcggaa acaccaaaag agattgctaa tgcgttggaa ttgggaacct attatgtgac
     1501 cgaaactaaa gctagtcatg ggttcgtgaa tacgttcaaa cctgtaaaag tcgaattgaa
     1561 gtatgccaat caaaccgtag cccctgttac cagtaacgta aaagggcaaa atcaagaagt
     1621 cactggcgaa accactttga cgaaagaaga caaagatact ggggataaag cacaaggtaa
     1681 ggcagtcttt gagggtgctg aatacacctt gtttactgct aaggatggaa aagctgttaa
     1741 atggagtgag gcttttaaac cagagttagc gaagggaacg aaaggttctg atgaaacggt
     1801 cactttgact ttagacgaaa agaaccaagt tgccgttaaa catttagcta tcaacgagta
     1861 ttattggcaa gaaacaaaag cgcccgaagg atatagcctt gatgaaacaa agtatcctgt
     1921 atccatcaaa aaggtggatg atagcgaaaa aaatgccgtg attacccgaa atgttacggc
     1981 aaaagaacaa gtgattcgct ttggctttga tttctttaaa tttgctggat cagctgcggg
     2041 cactaccgaa actggcttca atgatctgac ctttaaagtg tcgccattgg aagggaccaa
     2101 tgaaatcaca ggtgccgaag atgaagcgac cacagcttat aacgagcaat tagggtttga
     2161 tggttatggc aagtttgaaa atcttcctta tggggattat ttacttgaag aagtagaagc
     2221 cccagaaggt tttcaaaaga ttacgccatt agaaatccgt tctactttta aggaaaacaa
     2281 agaagacttt gtgaagagtg agtatgtctt taccattacc gaacaagacc aaaaacaacc
     2341 aattaaaacg gtgaccgttc cttacgagaa gctgacgaac aaagcatttt ctgttagttt
     2401 gaaccgttta atgctctatg atttgcctga ggaagaagat agtttaactt ctcttgcgac
     2461 ttggaaagac ggaaacaaag aattaacttc tctggattct accgagctag ttgataaatt
     2521 gagttacaat ctacatgaaa tcaaagaaga ctggtatgtg gtagcgcaag ccattgatgt
     2581 ggatgcgaca aaagctgccc aagaaaaaga tgaaaaagcc aaaccagtgg tgattgcaga
     2641 aacaagcgca acgttggcga acaaagagaa aacaggaact tggaaaattc agcataaatt
     2701 aaccgccgaa caagttttaa ataaaactat tgtcttattc aactatgtgt atgaaaataa
     2761 ggaagctttt gaagcaggcg ataagccagt agcgaaggat gttagcttga ataaccaagc
     2821 ccaaactgtc agttgtacag tggaacacca tgtttctatc caaacaaagg cccacctaga
     2881 aaacggttct cagaccttta ctcatggtga tgtggtggat atgtttgatg atgtgtcgat
     2941 tactcatgat gtactggatg gctcaaaaga agctttcgaa acaattctgt atgctttatt
     3001 accggatggt acaaacaaag aaatttggaa atctggcaag attgattatg aagtaaatga
     3061 taaagaattc accaaaactg tgcttgcgaa aaaagtagat accggaaaat atccagaagg
     3121 aaccaagttt acttttgcgg aaatcaatta cgataaagat gggaatatca atggaaaaca
     3181 caatgaagat ttgaaagaaa aatctcaaac cttaacacca aaagaagttc caaccatact
     3241 aagtacgccg aaacaaccgg aaacaccaac tgttccaagt ggctctcaag aatctagtcc
     3301 cacagtgaag accttccctc aaaccgggga gaaaaattcc aatgttttac tgttcattgg
     3361 ttttactttg atttttgcga cggcgggcta ttatttctgg aatcgccgga actaaggtga
     3421 tgagatgata caagtaaatc ctaagaacca acgtgcgcga ccgcctccta aaaaagtagt
     3481 cgcgctattt ttgtatccaa aactaacaat gaaaagagga aaagaagatg gaattgaaat
     3541 ttgtcgtgcc agatatggct gaaacctttg gaaagttaag ttatgccggt gaaggagaaa
     3601 ttataacaga aggatatgga cggaatacta cggtgattgg tcgcagttat catttgtatt
     3661 ccagtaagca acgggccgat gatattgagg tggtagtagc tgcggaagcc ggtgaaaagg
     3721 attttgatca agaccaaccg ctaaaagccg tgaatcccca tttggttgcc aaaggctatg
     3781 agattgagaa tcgtgggttt actgattacg tgttgtatgt tgatgattta gtgaaagcat
     3841 aggaggaaaa gaagatgaga ttaacagaag gtattgtcgt agattcaggt ttaacatttg
     3901 ggaagttgcg tttttccgca ttacgccgag aagtacggaa gcaaaatgag gatggaacaa
     3961 ttggtaatga ggtaaaagaa cgaacctata atttaaaatc ctctgcacaa gggcgaatga
     4021 ttcaagttag tatcccagct aatgtaccct tacgcgagtt tgcttatgat gcagaggtag
     4081 agttggtcaa tccaatcgtg gataccgttg ccaattatgt ttttcgagaa ggaactaccg
     4141 ttaattggtt cattaaggcg gatgatttag tcttgaagcg gcagccgaat caaggaaatc
     4201 ctacgaacca aaatgaaaga aagaaatagg tgaagattat ggaaattaat gttgaacata
     4261 ttttagattg tctcgatcaa tatggaaaag gcgagctgac tgaggaacaa ctcgttaaag
     4321 cattaacctc tgatgaaaaa atgtttctga ttatacatca aggtttactt ggagaaaata
     4381 gcgacagtga agaagactct gatgtactag actggttagg agaagaagag tctttcttta
     4441 tagttgtcga agtaaatgaa aacttatgtc ggcaggcaga atccattttg gaagaaattg
     4501 gtgtggagat gccggatgct attgaaggct ttttaaatca attggttgaa acgaaacaac
     4561 tcccctcctc gtagcagtaa atgactagca taactttttg aaattgccta atgaaatcct
     4621 attgggcttt ttcgattgga ggtggaaacc atcaaaatgt acaaaggaca tcgcatacgc
     4681 gcaggagatc agcacttggt ttatcacttt gttcttggct ggctactcgc tctgttcatc
     4741 ggttggatga gcgtctttta ttttcaagag tttagacaat ttgatatttc taagctatca
     4801 ctttctacta ttgaaatcgt ttggtctata aaagatttag tatgtctgct tggaagtctc
     4861 gctttttcag gagctatgat attgctttat attcactttt ttcttgatca ttggcgaagc
     4921 ttgtggcatc gacaaaagct agctcggatg attctagaga accattggta tgaagtgaaa
     4981 caaactcaaa gtgaaggctt tttcaaagat ttgaatagta gccgaaccag agagactatc
     5041 agttacttcc ccaaaatctg ttatcgaatg aaagatggat tactgtctat tcgcgttcaa
     5101 atttcacttg ggaaatacca agatcagctt ttaaaattgg aaaagaagtt agaaagcgga
     5161 ctgtattgtg agttagtaga aaaggaactc aaagactcct atgtggagta tactttactg
     5221 tatgatatga tcgccaatcg aattgggata gacgaagtag tggcagaaaa tgggactctg
     5281 cgattgatga aaaatcaagt atgggcttat gattctttac cccatatgtt aatcgctggt
     5341 ggcacgggtg gagggaagac ctatttcctc ctcaccatta tcgaagcact attgaagtca
     5401 gatgcagaat tgtttatcct cgatcccaaa aatgcagatt tagctgattt aggtacggtg
     5461 atgcctcatg tttattcaca aaaggaagaa atttctgctt gtgtggaaga tttctatgaa
     5521 cgtatgatgg ctcgtagcaa ggcaatgaaa gaaatgtcta actacaaaac aggagagaat
     5581 tatgcctatc ttggacttcc acccaacttt ttaatctttg atgaatacgt ggcttatatg
     5641 gagatgttaa cgacgaaaga aagtgcggtg attttgaata agctcaaaca aatcgtgatg
     5701 ttgggtcgtc aatctggttt ctttctgatt ttggcttgcc aaaggccgga tgcgaaatat
     5761 ttaggagacg gaatccgcga tcaattcaac tttcgcgtgg ctttgggtcg tatgagtgaa
     5821 ctcggatatt ccatgatgtt tggggaggtc gaaaaaaact tctttatgaa gcacatcaaa
     5881 ggtcgaggtt atgtggatac aggtggcagt gtgattagtg agttttatac gccacttgta
     5941 ccaaaagggt atgatttctt agattcaatt agtaaattag aaaagatgaa tgattgagtg
     6001 atcttcaata ctgactttat acttcaagta acgtataata cttttatata gtaggaagaa
     6061 aggggataat taatatggaa agtaatgatt cttatttaga gtatgcaaaa gcaaataagg
     6121 ataagtttat ttcttatatt atcaaaggga aatcccccga agttggacga gacgcagttt
     6181 ttatggcagg gagtcctggg gctggaaaat cagaggttgc cattggatta gctgagaatt
     6241 atgataacca tgtgattatt gatgcggatt actttcgcac ccagtttcct gattataatg
     6301 gaaaaaactc tagtctcttt caaaaagcat cttcttggtt ggtagagcag gcatttagat
     6361 atgtcttaga tcatggctat tcttttattc tggatgcaac ttttgcaata ttaagtgctg
     6421 aaaaaaatat ctcgcgggcg ttgaaaaaac attttcgagt gacgattttc tatgtttatc
     6481 aagaccctaa aatcgcttgg aaatttacac aagaacgtga aattgcagag ggacgtcatg
     6541 tgccgaaaaa ggcatttatt aacgcttttt tcaaatcaag agagaacatt gcaaaagtaa
     6601 agagtaaaca tccagatgta ttgcttcata tcataataaa agattatacg aataatattt
     6661 ccgaagttca ttatgctacg gataatctag aattattttt accactaggg tatagtagga
     6721 aagatttgga ggttcaatta catgactgag aaagaacaag taacgaaaat tgtaaagaaa
     6781 tacaataaga gcattgcgga tttatcggaa aatgctactg caaaagagtt caaaacggtg
     6841 attaaatatg tagccgatca ggccaatgaa aaacaacgga aattagttgg attaaataaa
     6901 aaatagtagg acaaattaat aatgtatgag ccaagagcaa aatgttcttg gcttttttct
     6961 ttgcaatgaa tgaggtaatg tatgagattt ttagatttat tcgccggtat tggtggcttt
     7021 cgtttaggta tggaacaggc gagccatcat tgtatcggtt tttgtgaaat tgataaattt
     7081 gctagacgta gttataaagc aattcatgat acaagtaagg aggtggaaat gcatgacatc
     7141 acaagtgtat cagatgagtt tattcaatcc ctcggaccag tggacatcct ttgcggcgga
     7201 tttccgtgcc aagctttttc aattgcggga aagcggcaaa gattttccga tactcgaggt
     7261 actttattct ttgaaatcgc tcggttcacc actcttctcc gacctaagct tttattcctt
     7321 gagaacgtcc gaggattgct caatcacgaa ggaggggcta cgttcgagac gatcctccga
     7381 acgctggatg gattggggta tgacgtggag tggtaggtgc ttaactcaaa ggcctacatt
     7441 ccccaaagcc gggagcggat attccttatc ggacattctc gagacgcatg taccgaacaa
     7501 gtatttccta tcattggacc gtcttcaaca tctgatcaaa acatcagaaa tttggtaaat
     7561 atcaatcctt ccaatcgagg aatgggtgga caagtctatg gttcagatgg cgtagcgcca
     7621 accttaacag gtgatgaagg aatcaaaatc gcactgccag caagggatgg aatctctgta
     7681 gcaggactat taccgggaaa gtttgaacaa ggtaatcgag tttatgaagt tactggaacc
     7741 gcccccacct tgtcaacgaa acaaggtggt acaaaaatca tgattcgaaa aaatgaaaca
     7801 gaatatcaag aggtgaagcc agaagacagt gtgaatctgg cttttcctaa ttccacaagt
     7861 cgccgaggtc gtctgggaaa acagagtgtt cataccttac taacaggaga ccaacaagcg
     7921 atcgtcacgg atcagtatca aattcggaag ctaacaccta gagagtgttg gcgacttcag
     7981 ggctttcccg attgggcttt tgatcgagat tctcaagtca attcagatag tcagttgtat
     8041 aagcaagccg gaaactctgt aacagttccg gtcatttttg atattgccag aagattaaaa
     8101 gagttggatg tacatggttt atgatgagtt gattggagaa atttattggg tgattggaaa
     8161 gattcagtca gacccagaat tagaagaaca attgcgacgg ttaaaatttg atattcgcaa
     8221 gaatggtgtc aaagtcgctg gcgatccata tttaatgaat gaggagacgg atgctcgaat
     8281 tgaaataaat caagtaattg cagaatttga gcggattgcg gaccaagcaa aagaaccaga
     8341 tattcgtcaa tacttgttag aaatgaaagc agagttggaa gtaaatggta ttacagatga
     8401 gtaacctaaa ccaacacaac tgactgttag gcgctgaccg cggaggattc gcgaagcaga
     8461 aatcctccgt ggtgctgtgc ctgttcatgg acggcgttag ccgaccatga tgtctcaccc
     8521 ccgtatctaa caggggggta caaaaacaca aaaaataagt aagcaaccat taagaatgct
     8581 tgttgtatca aagatagtaa gtaatttgta taggtgttaa ctcatttgaa aaagttaaca
     8641 ccttgaattt ggaggtgaga acttggcaca acggaattta gattatcggc tattaaaaga
     8701 ccgacgcaac gaatacggcg tttcacaaaa taaattggct acggcttgtg gacttagtcg
     8761 gccgtactta aatcagatag aaaatggtgg cgtaaccgca tctataaaaa ccatgaggaa
     8821 aatttttgac caactggaaa gctttaatcc cgatttgcct ttgacgttac tgtttgacta
     8881 tgtaaggatt cgctttccca caacggatgc acgaaaaatt attcaagaaa ttctccattt
     8941 gaaatttgag tatatgctcc atgaagatta tgccttttac tcctatcaag aacagtatgt
     9001 catgggtgat attgtggtga tgttgtctca tgaagaagat aaaggtgtgc ttttagaatt
     9061 aaaggggcga ggttgccgac aatttgaaac ttttttactc gctcaaaagc gtagctggta
     9121 cgactttttc gaggattgtc taaaggccgg tggcgtgatg aaacgcttgg acttggcaat
     9181 caatgatcga gtggggctat tggatatacc tgagctaacg aaaaaatgtc agaaggaaga
     9241 atgtatctcc ttgtttcgta cctttaaaag ttatcgttcc ggggaacttt taaaggctga
     9301 tgaaaaggac ggcatgggca ataccttata tatcggcagt ctcaagagtg aggtttattt
     9361 ctgtttgtat gaaaaagact atgaacaata tatcaagtta gggattccat tagaccaaac
     9421 tgagacgaaa aatcgttttg agattcgact gaaaaatgac cgtgcttttc atgcgattca
     9481 agatttgtta aaaggtcgta gcattgagag caccacgttt tccattatca atcgttactt
     9541 gcgatttgcg gataaagtag aaggtaaaag gcgaaccaac tggcccttga atgaacaatg
     9601 ggggcgtttt attggacgga atcggaaaga gattcaatta acttccgagc caaaaccgta
     9661 taccattgaa cgaaccttga attggctagg ccgacaagtc gcccctacgt ggaaaatggc
     9721 taaggagttg gatcgattga atcggacaac ctatatccag gatatggtac agaatgcacg
     9781 cttatcagac cgacataaaa agattttgga gcaacaaagt atggcaattg aaaatttgat
     9841 tatatgaaaa gaggaatcgt catgggaatt gtaaaagata tttttttggt atttggtggc
     9901 agtatgcttg gtgttactgt gatgtgcttg atgaatgcaa gtgcagcagc ggatcgagca
     9961 atggaaaaga aaggaaagtg acaagatgaa tttcggacaa aattt
//
LOCUS       DAZKNH010000092         9983 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000092 DAZKNH010000000
VERSION     DAZKNH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.092"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            254..733
                     /locus_tag="ACG8MG_002172"
     CDS             254..733
                     /locus_tag="ACG8MG_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFQ0376189.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            766..2994
                     /locus_tag="ACG8MG_002173"
     CDS             766..2994
                     /locus_tag="ACG8MG_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0376190.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3123..4766
                     /locus_tag="ACG8MG_002174"
     CDS             3123..4766
                     /locus_tag="ACG8MG_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0376191.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4839..5384)
                     /locus_tag="ACG8MG_002175"
     CDS             complement(4839..5384)
                     /locus_tag="ACG8MG_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFQ0376192.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5407..5892)
                     /locus_tag="ACG8MG_002176"
     CDS             complement(5407..5892)
                     /locus_tag="ACG8MG_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0376193.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5882..6694)
                     /locus_tag="ACG8MG_002177"
     CDS             complement(5882..6694)
                     /locus_tag="ACG8MG_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFQ0376194.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6833..8365)
                     /locus_tag="ACG8MG_002178"
     CDS             complement(6833..8365)
                     /locus_tag="ACG8MG_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFQ0376195.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8474..9505)
                     /locus_tag="ACG8MG_002179"
     CDS             complement(8474..9505)
                     /locus_tag="ACG8MG_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0376196.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3324 a         1845 c         1920 g         2894 t
ORIGIN      
        1 acaaaaaaga tgttggtgaa gttcaccaac atcaaaaagt atagagccaa aatattccaa
       61 catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt
      121 tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg
      181 catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg
      241 aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga
      301 agactatgat aaagttgatg gaagaaatgc attaaatatg gatgcaaaag ggctttcact
      361 gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca
      421 tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt
      481 agcgatctta gctgcgcaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt
      541 ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt
      601 agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact
      661 acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga
      721 gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca
      781 ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa
      841 agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa
      901 gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca
      961 caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt
     1021 gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag
     1081 gaacaagacc aagaattact agaagctatt taccaaacag atacacttga agctgtactg
     1141 acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag
     1201 ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct
     1261 gaaatcgttc gagcgattga aaaaggctat ttattagaga ttcaagagcc gactgtcatc
     1321 cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat
     1381 ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga
     1441 aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa
     1501 aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc
     1561 caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca
     1621 aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc
     1681 tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc
     1741 actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg
     1801 gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa
     1861 gaaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa
     1921 attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag
     1981 aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt
     2041 agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg
     2101 tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg
     2161 cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct
     2221 ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa
     2281 tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa
     2341 ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat
     2401 tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat
     2461 gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc
     2521 attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac
     2581 cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa
     2641 tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca
     2701 cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat
     2761 attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat
     2821 atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt
     2881 ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact
     2941 gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat
     3001 aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac
     3061 aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg
     3121 atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta
     3181 gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta
     3241 attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat
     3301 ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga
     3361 tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg
     3421 atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta
     3481 aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga
     3541 ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt
     3601 attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga
     3661 aagaagaact attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt
     3721 tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt
     3781 tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt
     3841 ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac
     3901 cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata
     3961 tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaacat gcattaagcg
     4021 cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg
     4081 ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa
     4141 aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg
     4201 aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga
     4261 aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca
     4321 ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg
     4381 ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg
     4441 tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca
     4501 ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc
     4561 cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact
     4621 taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac
     4681 tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag
     4741 ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg
     4801 cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca
     4861 ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa
     4921 cgaggtctga caggtacttg gacatgcttg atatgcatac cgattgatgt gtcaccgata
     4981 tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa
     5041 gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa
     5101 ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct
     5161 tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat
     5221 tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg
     5281 catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact
     5341 atttcagtca attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt
     5401 tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc
     5461 aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg
     5521 ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa
     5581 taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc
     5641 ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc
     5701 gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc
     5761 agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat
     5821 caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg
     5881 cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat
     5941 gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta
     6001 ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac
     6061 aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat
     6121 caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca
     6181 caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg
     6241 aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg
     6301 taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt
     6361 cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta
     6421 tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat
     6481 ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag
     6541 attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc
     6601 cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg
     6661 gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta
     6721 taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta
     6781 aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact
     6841 tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg
     6901 ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct
     6961 cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag
     7021 aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa
     7081 agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca
     7141 aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat
     7201 aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca
     7261 gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc
     7321 gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac
     7381 tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa
     7441 gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta
     7501 tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag
     7561 cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag
     7621 cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc
     7681 ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa
     7741 aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc
     7801 atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat
     7861 gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt
     7921 gttccattaa ataaagaata ggaatctgtt ttgattttgt tccatttgaa atttctctca
     7981 ctcgcttttt tgtctcatca tcaaataaaa tgaaagggtc ttctaaacga tcgataaata
     8041 cttccattgt tgttgttggt agatgataag aagtcatgac aggcactgca ccaaggtaag
     8101 acacagaaac agctagaagg tatgtgtcaa aagcggaact cttataaata ataactttgt
     8161 ctccggattt cactccaaga tgagccaatt gataagcccg tttcaacaca ctttcgcagc
     8221 attttttgta agttgtttca agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga
     8281 taatcggggt ctcggaaaag ttagctgcag cttcttgaaa attcgtaaat aagttcaaag
     8341 gggaatacgt tatggtctcc atcattcaca ctccattatc ttttgttaag taatatacaa
     8401 attcaagata atcgtaacat aatcctttat ataataacat agtttattgt gaaaaaaaga
     8461 tcatacattt gttttatatg gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa
     8521 aatcaaccat cttttcccag atatcttgaa aagaatggga tgagaggtat cgattaccac
     8581 ataaataatt taccgcgtca gcatcatcta aatatagttc tgcataataa cgatatgttt
     8641 ttctgcatag ttctggcgat acgtttttat atactgtaat tttgctcaaa atttggcgaa
     8701 tagtccgagg agaaatttgc tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg
     8761 tattttgtgc tgttaactta aaatagttct ccagaaatgg catcgcttct tttggtattg
     8821 gtactttatt cagtctgttt tcttttctat tcagattaag aatctgcttg gtcaaagata
     8881 catctttacg tttaagttct actacttcat ggatctttaa tccgcaatac agaagaatgg
     8941 cagtaattgc aaggttcctc ggtctattct tttcttcaaa gctagcttct ctgcttgttt
     9001 gaaagccttc aatcgggata aacaaatgct caaaatcttt gaaaagttct gcatcgatat
     9061 atcgcggagc tgctttggtt gtttccggta aacttgtacc caaaagagaa atctcttctt
     9121 caatcagtcc tttttgatac aaaaatcgta aaaaatgatt taatgaatat tgcttccttg
     9181 aaatcccgta atagctattt tcaaacaatt gctccgtttc ttggttcgct gtagtttgga
     9241 acgaacgagt atgtcgatag attttagcta agaaattctg ataatcttgt ttttctaaat
     9301 ggataatcag ctgatcatcc acttcttcag aaaatacttt tctcaaataa tcagtaaaca
     9361 gatgaaaatc ttttgcgtat tcatagcgac tgcctaatga aagggaagaa cgattttctt
     9421 ctaaaaactg ttgaaagacg gaagggagca agtgtgtacg ttgctttaac ttttcttgca
     9481 gttgttttct agttttgctg tccattacaa attccctcca atagtgttca ttttctatat
     9541 tttaccgcat ttcatcaaat gaagatagac agtttgtctt gatcttagga agcaaatttt
     9601 taactaagta ctagctaaaa gatatgaagt aagaagatac atttttagtt attttattag
     9661 tgcagtcatt cttttgatct tttaattgat ttatcctctt tttagctatg tggttacttc
     9721 atgagcaatg aaatggattt ggttcgttct aatggcagga ggctctttct tttatttccg
     9781 aaaaattaga aaacattttt tcagaatcga ttggctctcc atttattcaa taatggtttg
     9841 tctaacgata ttgttgggat aattcttaaa gaagtattag aaatagttgg tacttcatta
     9901 ctttacatga agatttattc tatggtagga gcaattatat tgagaattaa tcctttttta
     9961 tataagttgt ttgcttattg atg
//
LOCUS       DAZKNH010000093         9950 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000093 DAZKNH010000000
VERSION     DAZKNH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.093"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..1557)
                     /locus_tag="ACG8MG_002180"
     CDS             complement(46..1557)
                     /locus_tag="ACG8MG_002180"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0376197.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(1879..3231)
                     /gene="murF"
                     /locus_tag="ACG8MG_002181"
     CDS             complement(1879..3231)
                     /gene="murF"
                     /locus_tag="ACG8MG_002181"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFQ0376198.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(3384..4460)
                     /locus_tag="ACG8MG_002182"
     CDS             complement(3384..4460)
                     /locus_tag="ACG8MG_002182"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFQ0376199.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            4656..6401
                     /locus_tag="ACG8MG_002183"
     CDS             4656..6401
                     /locus_tag="ACG8MG_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFQ0376200.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(6681..7118)
                     /locus_tag="ACG8MG_002184"
     CDS             complement(6681..7118)
                     /locus_tag="ACG8MG_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFQ0376201.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(7102..8475)
                     /locus_tag="ACG8MG_002185"
     CDS             complement(7102..8475)
                     /locus_tag="ACG8MG_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376202.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(8797..9285)
                     /locus_tag="ACG8MG_002186"
     CDS             complement(8797..9285)
                     /locus_tag="ACG8MG_002186"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0376203.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(9307..9909)
                     /locus_tag="ACG8MG_002187"
     CDS             complement(9307..9909)
                     /locus_tag="ACG8MG_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0376204.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         2913 a         2119 c         1798 g         3120 t
ORIGIN      
        1 aaaaatgaaa gtttctctta aaaaagagaa actttcattt tatgtttaat cgttatttga
       61 acggattaca aatccgcgtt ttttatcatt gtgacgttta ccgccatctt tttggctgtt
      121 attatttttg ctataaccgc cttttgattt gtttccacga taattaccgc cgtcacgacg
      181 attacggttg tttccgccac cacgtttgcc atttttattg tatccttttt tctgcattgg
      241 caatgggcgt tctggtgtga tcttcactgg cactgcatca gctggatctt ttgctgtcgt
      301 ttttagcaaa agtgcaacta aatcttgtgc agaatattct tctaataatt tatctgctgt
      361 ttgcaggtat ttatctagac cattttctgc aagttttgtt tcgatttgtt caactgcagc
      421 acctaattga cctttgaatg cttctttttc ggttggtgga cgtaaagtcg tcatccgttt
      481 tttcgtcaag ttttcaatca catgcaagta gtccatttca tttggtgtaa cgaaagtcac
      541 agacataccg ccttttccgg cacggcctgt acgtccgata cggtgaacgt agctttctgg
      601 atcttgaggg atatcatagt tgtacacatg agtgacacct gaaatatcta atccacgtgc
      661 agcaacgtct gtcgctacaa ggatatctaa atgaccgctc ttaaatgaac gcaatacgct
      721 cattcgtttt tgttgagaca aatcgccatg gattccttct gcacggtaac cgcgtgcttc
      781 taatccgcgt gccaattcgt ctacacggcg ttttgtacgt ccgaagacga ttgttaattc
      841 aggtgtttga acatcaaaca agcgagtcat gatatcgaat ttttcgtatt cttttgcgcg
      901 gacatagtat tgatcgatca agtcagctgt catttctttc gctttgatct tcacatgatg
      961 tggatttttc atgaatttaa cgccaatatt cttgatagct ggcggcatcg ttgctgagaa
     1021 aagtaatgtt tgtctttgat caggaacttg tgaaatgatt ttttcgatat cttccaagaa
     1081 gcccatattc aacatttcat ctgcttcatc caaaactaat gtttgaacgg ttccaagttt
     1141 caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt gtacccacaa cgatatgtgg
     1201 acgatctttc aagccacgga tctgacggcc aatatctgct ccgccgtaaa cggcttgtac
     1261 acgtactttt ttgtcgcgcc ctaaacgata taattcttct tgtgtttgga tagctaattc
     1321 acgtgttggt gcgataacta aaccttgtaa ttcgtgacga tctggatcga ttttttctaa
     1381 cattggtaat ccaaatgcag ctgttttccc tgtacctgtc tgtgcttgtc caataacgtc
     1441 ttttccagct aatgctagtg gaatcgtttc tgcttggata ggcgttgctt cttcaaaacc
     1501 agcgcgttcg actgatttca acaattctgg tgataattct agttctttaa atttcaaatg
     1561 aatcctccta tatggttgtt aactgaggat gtagcttttt tgctaccccg tcgatttgtt
     1621 cggtcaaagt gtattgtttc tttttgacag tggccacgca cgtttttatc cattcttagc
     1681 ccctgtggca ccaccgaaaa atcactttgc gaagtttagg cattagaaaa gccaaacggg
     1741 tcgcgttcag ctttatcatt attatgacat ctggcttcaa caagttgttt ctatggcaaa
     1801 accagtccaa tttctcttca tctttcggca ataggttatt ctaacatagt ttttcgtttt
     1861 ctgcaagttt acagattttt atttcatttg ctgaagttgt tctaccactt caaataagcc
     1921 catgctgttg ctgcctttta gcataatact atctgttgct tcaagatttt gtttgatagc
     1981 atcaatcaat ttattctttt ctttttcaaa atagaacaca ggtagttggg ggtatttttg
     2041 gttcaattct tcttttagtg catgcatttg ttccccataa aggaaaatca tggcgaattt
     2101 ctcaggatcg atatgttcac tcatttgcgc atgcatattt aaagactctt tccctaattc
     2161 cagcatatct gctaacacag cgattctgcg tccttttgtt ggcagatttt taaaagtatc
     2221 taataccagt cccatggctg ttggatttgc attgtagaca tcgctcaaaa tagctgcacc
     2281 atttcctgcc gttagccatt cggttctgtt ttgagtgata gaaacatggg cgagcccttg
     2341 gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc gtatacgcaa tcaaagcatt
     2401 cgttacatta taacttccag gcagcgggat cgtaaatgtt tctccttcta ctacaaaatc
     2461 ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca tcgccatttc caataccgaa
     2521 tgttgtgact gtttgtacta atgttgccgt taaaggtgtc agcaacggtt catctgcggg
     2581 cacaacgagt aatccgtctg atacaagtcc gtcagtgatt tccattttgg ctttagcgat
     2641 tccttctcgc gacccaagat tttctatgtg tgcttcaccg atcattgtga ttgctgcagc
     2701 ttttggttga gccataagtg aaagttccgt gatttcattg gcgtgatcca tgcccatttc
     2761 caaaacgatc ttttctgtat cttggggcat gtgcaaaatc gtgtaaggaa gaccaatatt
     2821 attgttgtaa tttccttgcg tcttatatgt tttgtatttt tcagacaaga cagacgcggt
     2881 tagatctttc gtggtggttt taccattgct tcctgtaatt gcaacgactt ctgcgcctac
     2941 tttttccaag taataggttg ccaattgctg catggctttc aaaggatctg ctacaggaat
     3001 aactggaaaa ttttttggag cttgttcaac aggcaagctc caaaatgaag cagatgctcc
     3061 gtttgtgatc gcttgctgga taaattcatg tccatctcgc gtaccagcaa gaggaacgaa
     3121 taaacttcct tcggttatct tccggctgtc aaattcgaca ttggtaatct ttgtcagtgc
     3181 agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt gttagattca ttgagatctc
     3241 tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg cgggttcgca tgactctcac
     3301 ttccaagtaa atttctctat tcgtatttag aagctagaat gaacgcggtt acgcaatcac
     3361 tccagcctct tctttatttc tctttattca tttttttcaa aaaaagattg acgctgatgg
     3421 tatcgattca ttcctaactg gatcagttct tcaatcaaat caccgtattt caagcccata
     3481 ttttcccata agagtgggta catactgaac tccgtaaatc ctggcataga gtttaattca
     3541 ttcaggaata attcattttt atttgtcaaa aagaaatcgc accggctcaa tccgcttcca
     3601 cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt tttgataaac ttcttctggc
     3661 acttcggctg gaatctgcat ttcgatttta ttattgatat attttgcttc ataatcatag
     3721 aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc gaacgtcttc atttcctaat
     3781 acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa cgattgctcg agaatcatac
     3841 tgataggctg ttgctaaagc attttgcagc tcttctcggt tttctgcttt tgtaatgccg
     3901 acactagaac ccatattcgc cggtttgaca aacatcggat aaagcaaaga accttcacat
     3961 tgatcaaata cttttttagg attttctttc cattgattct taagtactgg tacataagga
     4021 acttgcggca caccagcagc ttgtaaaata tacttggtca tgattttatc catggcacat
     4081 gcactggtca atacgcctgc gccgacataa ggcatattca atgtctctaa gaagccttgg
     4141 atcgttccat cttccccgtt tggcccatgt aaaactggaa aaacgatggc tccttcttct
     4201 ttgatttcgc ccggattgat cacttttcct gtaaagcctt cctctgtctg tccacttggg
     4261 tcccatgaaa gatgcaagac atctttgctg gcaggttttt ctgttagtaa tggacctttg
     4321 acccattgtc cttcttttgt aataaatacg agttgaactt ggtaataatt ataataaatg
     4381 gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat gctctgcgct gcgtccgcca
     4441 tatagtaaag taatcttcaa aagattgtcc tcctgtattc taaatcgttc agtgatttac
     4501 tctcatattt tagcacaaaa caaaaataaa cgaataactt atttataaaa aaacgagcca
     4561 gcttctaaaa gaacgagcgt catttatttt ttgtcagcta gattatttgt cccgtttttt
     4621 ataaaaagtt atgataaaga aaaggaggga tgatgatggc gcaaacaaat ccacgcagcc
     4681 gcttggcaga aatcattcga gtgttcatca attacaatgt agtgccaaac tttgtacagc
     4741 aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact aggccccacc ttcatcaaga
     4801 taggacagat gctttctgtt cgagaagatt tgctttcttc tgccttcact caaacattca
     4861 aaacattaca agatagtgta cctagcgaca cattttcaac ggtcaaaaag acgatcgaaa
     4921 cagaactttc tctttcatta tcagatatat tcgatgattt ttcaaaatcg ccttttgcct
     4981 ctgcttctat ggggcaggct catcgtgcca agctgaagaa cggtgacgcg gtagtcgtta
     5041 agatccaaca ccctaacatc gccgaagaga tccgacttga tcttcaacta tttgaacgag
     5101 cgatcccttt gatcaaatac attcctgaaa ctagcgtagt tgatctaaaa ggggtactcc
     5161 aagaagtaaa acgttccttg atcaatgaaa tggacttctt aaaagaaagt caaaatggcg
     5221 aacaattcta tcaaaagaac aatggctgga aagagatccg cagccccaag atttatgacg
     5281 ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc tggtaaaaac ttgaatcatt
     5341 taatgaacgc cgaaaacaaa actgaaactt ttatcactgg aattcaaaac aagcagctga
     5401 aacaagaagt tgcaaagctt ttagttgaga actttatgaa acaggtgttt gatgatggtt
     5461 ttttccatgc tgatcctcat ccagggaatc tgctattcca tgtactaaca aaagaagaac
     5521 agacacaagc tagccgaaaa actgaaacag tccatgaaaa agagttcggt tcctttgctt
     5581 ttcgagcctc cacttctgca gaagaccctg ttgcgccata taccatcaac tacatcgatt
     5641 ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa actcacacaa gccgttttag
     5701 cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt ccttcgactt tgtcagcaag
     5761 aaggctcgtt tgatgaaagt cggttccacc aagaactgac aagttttctt gaacagtatt
     5821 atgattctcc tattgatgaa atcaacttgc aagaagtatt cacacatgtc gtgacgattt
     5881 gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct tttgctgaaa gcatttggga
     5941 cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc cctcatggaa gtcgcttcgc
     6001 cttttgctca acattatttt ctcactcatc tggatgtaga agatacattg aaacaaagcg
     6061 gattagactt attggaaggg atgaaggctg cgcctaagat ccctcaacag ctccaccatc
     6121 tattagaaat gtggacttcg ggacaaggaa aagtcaattt ggaattgaaa aagcaagaca
     6181 aattattgag caggatcgaa agtatgatca atcggcttgt tttcggtatg attttagctg
     6241 cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc tgtagaaaac gcagaggtcg
     6301 tttccttatt agggatattt acgtatgcta ttgccgcatt cgtcattatt tttttggcaa
     6361 tcgatgcatt gatccaaatg tataagaaga ggaaaaagta gaaaaaggtt gtgacagaaa
     6421 gaatcagctt caagaaataa gaaggggttt tacataaatt gtttctcaaa tttttgtgaa
     6481 aattcggctt atttttcgaa gaggctactt ctgaaacacc gagaataaaa aatgtgaaaa
     6541 agctgttctg cacccagtaa taagaacaat caaaaaggga acatggaaca catttatcct
     6601 gtttccctgt tccctttttg atgtattcga gcataaataa aataccttcg ttacctacta
     6661 tcttctgtac ttggtaaacc ttagtcaatt gcctttttta acgcttgacg aaaagacata
     6721 tgatctttct ccgtaaaggg ttttggaccc tttgtcctta tgccagcttt tcttaacttt
     6781 ccactatgat aacgttgttc tagaagcgtg ccgatattcg cttcgtcaaa ttcttttcca
     6841 gagtgatgga acactcgcac acccttcggc aatacaagtg aagctaaacc ataatcttgc
     6901 gtgatcaaaa agtcattctt cttaaccata ccgacgattt tataatccgc actatcagct
     6961 ccgcggtcta cgtagacgat ttggacatga ttagcgtatt ctttggtcgt ataatggtcc
     7021 acactggtca cgataaccac gtcaagacca taggattctc ctaattttat gacatcttct
     7081 tttactggag aaccatctcc atcaatgaat agcctcattc tttttcgcct tcttccataa
     7141 atgataaaca gttactaatc cgctcagtaa aagaccaaca ttgatccatt ttgccaagat
     7201 aagcagttgt acactttcta tcggcactga ttcatggttc agcgtgatca ataatagatg
     7261 gattggctct cccatttctt ttgctaaaac aaacaaaagc gtcatcaata aaaactggtg
     7321 gatcacactc cctttatttt gagtagtata cttgacaacg atccggcgat ccattggaat
     7381 agctgtatca gtttgataat attccttatt tagccagctg cctgttacta cgtgccagaa
     7441 actaagcaga aagacaccta tcgcaaaacc atttggaatc aagaggacca ataaagaaga
     7501 actcagtcca agcaattgca tagccaatgg agaccatgtt aaacgtttca aaatccatgg
     7561 tccgactatt tttgctgcaa tcaagcctgc tgcataaggc aaatacataa aaatcgaaag
     7621 tttttctaat ccgtacagta tgccaacata aaaactgcta aataaaaaaa ggaaattccc
     7681 tagcattcca tttagaaaag taaacagatt tagccagctt ggcaacttcc attccttctt
     7741 ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc aagaaaccat aaatagcaag
     7801 atcaaaaagc ctttcatcga ataacaatcg ggcatttctc aaaagaaaca ataagccaaa
     7861 gaatatcaaa aataaaacaa gctctcgcgt agctaccaga tttcttttga tgtctttgaa
     7921 atccagttcg taacgcggat aatgactaac cgtatggtag gccatgacgt aaaaaagtgt
     7981 ataaatagta aagcttgctg caatctgagt cggtgcaggc aactcaatcg cttgatacag
     8041 gagaaatagc cagatagctg caaataagta ctttttccca tccatattga taaaaccttg
     8101 cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca agccatgctg cactcagccc
     8161 tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat acgcctaata ttccaagcaa
     8221 tgaaccagca ccaccaatca gcagagcgat catcagcaac gtgtaactat ctagaccaaa
     8281 tcgtagactg ttgactaaaa atagtccagt catccgaaag gcataaaaca gcgtaaatgg
     8341 caaaatcgtc agccaattat gcgagtctgt tagtcccaaa aataaaagat aaggaataaa
     8401 aataaccgtg ttacttaaga taaagggcgt tgtttgtgta aaattacgcc caaatcgttt
     8461 gagtttttcg aacatgacaa tggctcctta aacagtctta taactaatct gctcttcgat
     8521 tttagcatta aaggggaaat aaaccaattt tacagaatat attcttgtaa aaaaaaatac
     8581 agaaagaagc ataagcttct ttctgcattt ttacggtact tgattactca agcagacaag
     8641 cccttctcca caaaagtctt gcccgacacc cctgcatccg gcaggtgata aagatagaaa
     8701 tcaattatcc tgtgcctcca aaatcaatta ggagctctgt aaaccagagt ccttctcgac
     8761 ccgtcgcata aatttacttt aacacagttc ctatgtttat gcgagagctt ttttcatttt
     8821 ataatcgacc atttgaaatc cttgaccgat atctgaaata agttcttctg taatctcaaa
     8881 tccttcatgc ttataaacct cgattgcttg atgattcccc tgattcactc gcaaaaagat
     8941 ctctctttcc tttgtttgcg cagcaagttc ttcataccag cgaaaaactg aagaagtcaa
     9001 tccttgacca cgtactttat ttagcaaata taatttactt aaataaattt ttccatttat
     9061 ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt tttaataaaa agtaggacac
     9121 accttctgtc agttcttttt gaatctgttt ttttgactga taagttttca gcatgtaatc
     9181 cacttgtttt tgaccaatga tcggcacaaa aacttccggc catacttctt gtatgactgc
     9241 catcagctca tccagttcat tttctgttac ttttattttt tccatacacc cacgtccttt
     9301 tttacattat aaatgacctt gcgcttgatg gaataaaaag agcatgatca tgctatacac
     9361 acccaccgac cacggtttca agaaaatcac aatctgcata aattccctaa aagataattt
     9421 cgtcaaacca gctactagac agatcaagtc gtctggcgca aagggaagca gcaaggtaac
     9481 aactagtact tttttaatat ctttcgtttt tcttgtcagc aattcatcaa atttctgata
     9541 cttttttggc gataaaataa gctggacaaa ggctacgcca tagtaacgga caagcaaaaa
     9601 tccgattgct tccccaatca ctaggccaat acaactatat aaaagcccaa atccattgcc
     9661 aaacatcagc atgcccgcta ctgaggagat ccctcccggc aaaataggaa tgatcacttg
     9721 gatcacttgc aacagaacaa atatcatcac tgcatacttg ccaaattgct ggataaacgc
     9781 ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa gcataaagaa tcaacacaaa
     9841 gaaaaaaaac agaccaataa tgggcatgat ctgaagacat ttttgtaaaa ctgatccttt
     9901 tttagacatc tatattcccc acatcttttt tcatttatta aaactgcctc
//
LOCUS       DAZKNH010000094         9886 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000094 DAZKNH010000000
VERSION     DAZKNH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.094"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..2229)
                     /locus_tag="ACG8MG_002188"
     CDS             complement(109..2229)
                     /locus_tag="ACG8MG_002188"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0376205.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2459..3637
                     /locus_tag="ACG8MG_002189"
     CDS             2459..3637
                     /locus_tag="ACG8MG_002189"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFQ0376206.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3653..4411
                     /gene="aroD"
                     /locus_tag="ACG8MG_002190"
     CDS             3653..4411
                     /gene="aroD"
                     /locus_tag="ACG8MG_002190"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFQ0376207.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4434..4919
                     /gene="ybaK"
                     /locus_tag="ACG8MG_002191"
     CDS             4434..4919
                     /gene="ybaK"
                     /locus_tag="ACG8MG_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFQ0376208.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4990..6243)
                     /locus_tag="ACG8MG_002192"
     CDS             complement(4990..6243)
                     /locus_tag="ACG8MG_002192"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0376209.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6360..7709
                     /gene="gdhA"
                     /locus_tag="ACG8MG_002193"
     CDS             6360..7709
                     /gene="gdhA"
                     /locus_tag="ACG8MG_002193"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFQ0376210.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7821..9167
                     /locus_tag="ACG8MG_002194"
     CDS             7821..9167
                     /locus_tag="ACG8MG_002194"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFQ0376211.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9475..9861)
                     /locus_tag="ACG8MG_002195"
     CDS             complement(9475..9861)
                     /locus_tag="ACG8MG_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0376212.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3148 a         1800 c         2025 g         2913 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcctaattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
      121 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
      181 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
      241 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
      301 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
      361 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
      421 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
      481 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
      541 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
      601 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
      661 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
      721 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
      781 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
      841 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
      901 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
      961 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     1021 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     1081 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     1141 gaatgtacca gcgttcccat ggaataccgc actagtgtaa ccttgtgttt gacttaaaat
     1201 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     1261 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     1321 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     1381 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     1441 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     1501 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     1561 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     1621 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     1681 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     1741 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     1801 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     1861 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     1921 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     1981 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     2041 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     2101 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     2161 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     2221 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     2281 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     2341 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     2401 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     2461 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     2521 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     2581 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     2641 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     2701 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     2761 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     2821 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     2881 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     2941 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     3001 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     3061 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     3121 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     3181 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     3241 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     3301 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     3361 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     3421 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     3481 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     3541 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     3601 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     3661 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     3721 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     3781 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     3841 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     3901 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     3961 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     4021 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     4081 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     4141 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     4201 ggagttgact agacgggcaa atgcttttgt ctccatgcct attattacga tgtccatggg
     4261 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     4321 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     4381 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     4441 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     4501 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     4561 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
     4621 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
     4681 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
     4741 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
     4801 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
     4861 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
     4921 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
     4981 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
     5041 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
     5101 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
     5161 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
     5221 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
     5281 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
     5341 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
     5401 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
     5461 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
     5521 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
     5581 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
     5641 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
     5701 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
     5761 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
     5821 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
     5881 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
     5941 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
     6001 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
     6061 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
     6121 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
     6181 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
     6241 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
     6301 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
     6361 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
     6421 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
     6481 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
     6541 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
     6601 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
     6661 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
     6721 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
     6781 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
     6841 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
     6901 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
     6961 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
     7021 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt gtttccggaa
     7081 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
     7141 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
     7201 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
     7261 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
     7321 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
     7381 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
     7441 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
     7501 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
     7561 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
     7621 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
     7681 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
     7741 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
     7801 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
     7861 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
     7921 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
     7981 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
     8041 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
     8101 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
     8161 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
     8221 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
     8281 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
     8341 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
     8401 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
     8461 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
     8521 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
     8581 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
     8641 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
     8701 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
     8761 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
     8821 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
     8881 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
     8941 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
     9001 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
     9061 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
     9121 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
     9181 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
     9241 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
     9301 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
     9361 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
     9421 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
     9481 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
     9541 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
     9601 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
     9661 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
     9721 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
     9781 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
     9841 caaccctaaa atttttttca tgaaagatct ccttttttat ttttat
//
LOCUS       DAZKNH010000095         9674 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000095 DAZKNH010000000
VERSION     DAZKNH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.095"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..301
                     /locus_tag="ACG8MG_002196"
     CDS             107..301
                     /locus_tag="ACG8MG_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376213.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            570..869
                     /locus_tag="ACG8MG_002197"
     CDS             570..869
                     /locus_tag="ACG8MG_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376214.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(966..1400)
                     /locus_tag="ACG8MG_002198"
     CDS             complement(966..1400)
                     /locus_tag="ACG8MG_002198"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0376215.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1632..2342)
                     /locus_tag="ACG8MG_002199"
     CDS             complement(1632..2342)
                     /locus_tag="ACG8MG_002199"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0376216.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2332..3192)
                     /locus_tag="ACG8MG_002200"
     CDS             complement(2332..3192)
                     /locus_tag="ACG8MG_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFQ0376217.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3197..3835)
                     /locus_tag="ACG8MG_002201"
     CDS             complement(3197..3835)
                     /locus_tag="ACG8MG_002201"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFQ0376218.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3850..4149)
                     /locus_tag="ACG8MG_002202"
     CDS             complement(3850..4149)
                     /locus_tag="ACG8MG_002202"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0376219.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4192..5676)
                     /locus_tag="ACG8MG_002203"
     CDS             complement(4192..5676)
                     /locus_tag="ACG8MG_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0376220.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5696..6157)
                     /locus_tag="ACG8MG_002204"
     CDS             complement(5696..6157)
                     /locus_tag="ACG8MG_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0376221.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6175..7242)
                     /gene="ulaG"
                     /locus_tag="ACG8MG_002205"
     CDS             complement(6175..7242)
                     /gene="ulaG"
                     /locus_tag="ACG8MG_002205"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFQ0376222.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7460..8230
                     /locus_tag="ACG8MG_002206"
     CDS             7460..8230
                     /locus_tag="ACG8MG_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0376223.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8503..9330)
                     /locus_tag="ACG8MG_002207"
     CDS             complement(8503..9330)
                     /locus_tag="ACG8MG_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0376224.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         3014 a         1967 c         1507 g         3186 t
ORIGIN      
        1 tttcaaattg attcttttta tttatcataa agatatagat acataatcat tttcacttac
       61 atatatttga atattaacta aaaacagcaa gaaagaagag gatcaaatgt attatgtaga
      121 agtaaaaaca aaaggcgtca aaaacaaaca atatgtgaaa ggtatcagta acgaatatcc
      181 tttacttggt tcatggaaag aagctgctcc tttttccaaa ccatgtgcca taaaaataaa
      241 gaacgagcta gaaaaagaac tgacttgtgg taaagctgtt gtagatatca tcgaaaagta
      301 aatccaatgt atagaaagga agatcacaca tgaaaagcga cgtacaaatt gctaagatta
      361 tagaattaga agaagaagac gcagtgttga caattgaaaa aaaataaatg tttgtatacc
      421 atatgattat ttcaatatgt atcctggaat tggagatctc gtagagctat atcaagatga
      481 atcaaatata cttgtcattc atcatgaaat atagagactt ataaaattgt ttatttttat
      541 aatgtatcag tatagaaagg aaataaaaca tggattatac tgttgaattt gacagtgaac
      601 tacttaagca atttccacaa gaagaacatt tcatgtataa agaagcaatt tcccacaaag
      661 tgacagagta tttggagcat aatttttttc gtataaagcc agtgcgtact cgttcggcaa
      721 aaggaatata cgagatgaaa gtcaaaatta ataaaaaaaa ttatcgcata gcatttgctt
      781 taaaaaaaaa tcatgtgttc attttttata ttagttcgaa tttgcaaaaa attgtatttg
      841 ataaagaggt gagtaaaaaa ataaaatagc tgagcattgg aggatagatt gagtgatact
      901 aagtctatcc tcttgttttt ctaaagaact aagctagaat tttttctttt gtacgttatt
      961 atatgttatt tgtcattacc aaaaagcacc ttttttcttc tagtatctat gacataagaa
     1021 ataccaccca tttcctttac ttgaacaaat cctactgatt cataaaattt ttttgttttc
     1081 tcagtatcat ctgtcaataa ttcgatttgg actggtccat aactagtgat cttctccaaa
     1141 gcttcattca aaagcacttt gcctattcct tggcgctgat acctcggatg aatgaggata
     1201 tcttgaataa atgcgatcgt cgcaaaatca gtgatccaac gaacgattcc gataatcttc
     1261 ccttccccat tacgctttat cagagattca gattgttcaa atgctctagc taaccttgca
     1321 gtgtcttttg tgtaattcgt ccaaccaacg ctcttatata attcagaaat ctcatcgatc
     1381 ataagggaat caatattcat ttttttcttc ctttcttata caattctccc tctaataata
     1441 cagttctttg actatttaat ctatcacata taactctcct gtaaagattc tctcgattcc
     1501 ttaaaattcc tctttccttt tagaatgcat actttttgat aaataaaaag gttgtgataa
     1561 attttcttca caacctctgt ttctctctat caagtgttat cttttaattt tctaccctta
     1621 ccctagcatc tctattgccc gtagtatgca tactttccat gtttgcgaaa ataatgttta
     1681 tctagtaaat attgcgggat ttcacatata ttggggttga tcagctcgtt ttctttggcc
     1741 atcagacaaa tttcatctaa gatcaaactg ttttctactg ctttcatagg tgaatccccc
     1801 catgtgaacg gtccatgtcc atagacaagt actccaggga cttcgtttgg atctaatttt
     1861 ctttccttaa acgtctcgac aatcacattc cctgtatgaa cttcgtaagc ttctcttact
     1921 tcttctttcg ttaattggcg tgtacaagga accttaccat aaaaagcatc agcatgagtc
     1981 gtgccataag ctggcaaatc gcgtcctgct tgcgcccaca tgacggagtg cgtggaatga
     2041 gtatgagtaa tagcagttat atcttcaaaa gtttgataca aaacaacatg tgtggggaga
     2101 tctgaagatg gttttaagct atcttcttct attatatttc ccgacaaatc tgttaccacc
     2161 atttgatctg cttgcatgca ttcatatctg acaccgcttg gcttaatgac gatgactcct
     2221 agctcacggt ccacttcact cacattgccc caagtcaatt tgactaatcc gtatcgcggc
     2281 aatgccagat ttgcttcaag gacacgttgc ttcattgctg caattttttc ttcacaagac
     2341 atcatagcca gcctccttca atttagaact taagtagtct tttgcctgct ggatttctct
     2401 tcgaaagtct tggctttttt cactccacat ttcaatcaga aatgttccat cataatctag
     2461 acgtttcaaa tgtcttaata atccaagaaa atcaacacaa ccgctaccaa aaggaacatc
     2521 acgaaactgg cctgaatatt cttttgttac agggtaagta tcttttaagt ggatagcagt
     2581 gatataatcg atccctttct caagttctcg agccggatta ttttccggcc atgcggacaa
     2641 attgcctagg tctggatata cttgtagaaa aggtgaatga atctgttctt tgatttctaa
     2701 aaattttgaa atggagttca taaatggatc atccatgatt tccacggaaa gaatgatgcc
     2761 atatttagaa gcttccttta cgctttcttt tattccttcg ataaagtctt ctctggaatt
     2821 cattgatttc tcttcataat aaacatcgta tccagctatt tggattactt tgacgcttag
     2881 attatgcgcc aaatggattg cttttttcat tagttgtttc gctgcttttt tcttttctgg
     2941 atcagcagaa ccaaacggaa atcgccgatg cgcacttaaa caaatcgaat ggatacgtac
     3001 accggagctg aacatctctt ctctcagctc agctatcttt tcttcagacc aatctaatcg
     3061 tgctaaccgc tcatccgtct catcaatcga catctcgata aaatcgaagt ccatttcttt
     3121 tacaagggaa aaacgttctt tccatgaaat atctttgggt aacgcttttt cataaatccc
     3181 aatttgagcc atttccttac ccccagatac gaatgatctc atctttgaag ttttgtgcgg
     3241 ccttcatcgg atcatttgct gctgtaatac cacgaccagt gataaaggta tacacatcga
     3301 tcccttcaaa taagtgtaat gtgtgtggat ttaatccacc agttacagaa acattaaagc
     3361 ctagttcaat cagttttttg atcttcgata aatctttttc accccaagtt tcaccagata
     3421 acagcgcatc acggctttga tggtagatta cctgccggat accgatgtta tgccaatcca
     3481 tggcttgttc aaaagtccaa ttgccgtaga gttctacttg caattcacct atttcttttt
     3541 gtgctgcctc catagttgga atcgttgcac aacaaatgac tgtcatccag tctgctcctg
     3601 cttttgcaac attactcgcc actgttcctc cagcgtctgc acatttcgta tcagcgacta
     3661 gttttttatc tgggaacata ctgcgaatac agcgaatcgc tttttgtcct tcctgtaagc
     3721 aaagaattgt gcccacttca ataatatcta ctacatcacc aactttcata acatctccta
     3781 atgcatgttc taagtcattg tgatctaacg caatttgcaa attcggcctg ctcatcttag
     3841 ttccttcctt tacttgtttt tttggtacca ctttgtttca gcaaattttt cactgatttc
     3901 tttgactgac atcacatttt tgataccgat aactgtaact ccttttttct ctgcctgagt
     3961 aaacatattc aaaaaattga ctggcgtaaa aactacatcg tattggccag ctgaactttt
     4021 tccctctgaa atggcacaat gatggatttt ggtaattggt acgtttagtt ctttcattgc
     4081 tttttccaca cttctcatca tcatcaagct tgttcctgat ccatttgcac acgatactaa
     4141 aattctcatc ttaatccctc cattatgagt gttgtctaat cagattttct tttatactct
     4201 ctgcttttct gcatatgtgt catagtcttc tacaatcaaa aaataaccat caggattttt
     4261 ccgatattgt aactgcggaa tagccaataa accaatgaca actacggcaa ttccagcata
     4321 acctaggact ttcatgatta ctgtaaacaa tggccaagct gttgcccagt cgaacatccc
     4381 gatataacca ccgtatgttg acatcccaat ccatgtagcg aataatgctg aaccgcctac
     4441 ttgtatgatt cctgaaagaa acgggaacag gcaagcggct ttgaatccac ctcggttatt
     4501 ggcataaact gctataacgg cattatcaaa gaacaacgga atgaaaccgg ctatgacgat
     4561 cgttggtgat ttcaataaaa tcagtaaacc aatcatcaaa aactgtccca aagcaccaaa
     4621 caagacccca atcgttaccg catttggcga accaaagcca aaagtagcag ctacgtcaat
     4681 tcctggaaca gctcctggta aaatcgtgtt tgagattcct tggaaagatt gagttagttc
     4741 atcgacgaat gtccgaacac ctaattgcaa gatcgccaga tataccgcaa aatttaaaga
     4801 agtcgtcata atgtagaata agaaactctg cccttcttgc atgaattgcg cttgaatcaa
     4861 ataatctttg cctagtacaa tcagaatgat cccaaagaaa aatagcatga gtaacgaagt
     4921 agcaaccata ttctcattga aaatcgacaa gaatcctggt aatttgatat cttcaatttt
     4981 tttgtcttgc ttgattgatc tatttttctt ttcatcccgc tttttcatcc actcagctaa
     5041 ttttgcaaat ataaagattc cgaacatttg ctgatgtgcc actgcaaatc cggcaccttc
     5101 tgtcaattcc tgcgtgattc ctactgttaa gttagaagct actgtccaat aacaacctaa
     5161 aatcagtccc ataaaaatca atgcttgtat cctacctaga tcaggaaagc aaaacagtaa
     5221 aatccaaaaa gcggtagctg cttgttgaac ctgaacgttt ccggtagtga aaacggaacg
     5281 cagtttcgta tatttctgaa aacggactaa taagatattt aaaacaaatg caatcagtaa
     5341 taaaatcatc acatctccaa aggtacggcc aaatgtctcc attaatccag catccacagc
     5401 attttgtcca aaataaggat ctgtaaccat ggcatccaaa ttgaaccgtt cctttagtcc
     5461 aaccaagatt ggccgaaaat tattgaccaa accaccagag ccaacagtca aaataaagta
     5521 accgaccgtt gcctttaaga atccagcgat gcattcatga agcgggcgtt tcagcaaaat
     5581 atagcctaat aacacgataa aaccaatcag gtatgctggc tgcgtcaaga tatttgttgc
     5641 aaaatacgtc caaatgctta acaaaaattc ccccatcttt tttctcctct atcaattaat
     5701 tatattctgc catcacaact tggtaatcat cgatggagtt cgtagagatg agacgctcaa
     5761 ccaatccatc tgtcattaat aattcggata atttttggat attttctaga tgttcttctg
     5821 gatcttttgc tgcgagtgta aaaaacaaac tagccgtttt ctcctcattc tccccttcac
     5881 tttcaaaacg gattggctgt ttcaacttgg taaatgaaat agctgttcca aaaacaccat
     5941 cacttttttc tgaagaatgc ggcattgcta cttgaggtgc gatcacaata taaggaccgt
     6001 atttttctac acaatggatg atctcctcca catacgtttc atcaataatt tccttatcga
     6061 tcaatacaag acagctttcg gcgatagctt cacgccaatc cgtgatttct ttatctgggt
     6121 aatgaatcaa ttgatggtca aaaaagtatt tcagcaagaa actccccttc tttcttataa
     6181 aaacgacgga aacggtgtat cattttcaat cgagaagaca tcttcaaatc ctcgataaaa
     6241 attaaactca agatcatctt tgtttgtcgg atagatgaat ttcccgccta cttgccagat
     6301 ataaggttta aatccatact tcaggcgatc ctttttgaat ttccaaattt cagtgatctc
     6361 ttttggatca gccataaaat tagaccaaat atcgtagtgg acaggaatca ctacttttgc
     6421 attcaatgac tccgccatgc gcagcatatc aacagaagta actttgtctg ttatccctcg
     6481 tgggttttca ccataggccc ctaaacagac atcgattttg tgttcattac catgtttggc
     6541 aaataaattg gagtaatgtg aatctcctgc atgataaaga ttccctcctg atgtttcgaa
     6601 taaatagttt actgcaattt gatccatctc ctgaggcatt tttcctttta atacctcttc
     6661 tggatcttca caagtgacca acgctgtccg atcaaacgct tctaaagcta ctacattgat
     6721 gtctttgatt ttgactgcat cacctggctt tactgtaatc gttttttctt caggaattcc
     6781 ccatttttgc cacgtgcgaa ccacttcttt tggcccaatg aatgttgctt caggacaatt
     6841 ttgatgaact gctgcggctg tatggatatc tagatgatcg gaatggatat gtgtaacgat
     6901 taaagcgtct acatttttta tcgcaaaagg atcgattacg aaaggctgag tacgtaaatt
     6961 aggctgcata ttttgaacgc cactcatacg catcatctga tggccttttt tcatcatgcc
     7021 attcccatga ctacgttttc ccgtcccaca ccatagatca cataaaatat tcgttccttc
     7081 atgacttttc agccaaattc ccgtacagcc cagccaccac atagcaaaag ttccaggatc
     7141 cactacttct gtttcgattt cttcattcaa gtatgtgccc cactcaggaa atgtttctac
     7201 taaccacttt tcttttgtta cttcattgac agttttactc atttgcttgc tccccccata
     7261 tcgttgttcc aacttacaag taaatattaa tgtgtatcag catccatgaa aaccctttta
     7321 tatgaccgtt tgttttttat atgattttct ttcagtaatc aatgattgtt tcagtataat
     7381 aaagacagat atgaatgttt aaatgaacaa ctagtcaaat aaatccatgg atcaagatgt
     7441 ccctcaggag gcaaaagtca tgaaaaactc acttgtcaat attgaaaaac gtcaaaaaga
     7501 aattttggaa ctgcttcagc agactgaaca actatcaact ttcgaattag ctgaatccct
     7561 tgatgtatct attagtacga tacgaagaga tttaaattta ttagaagaaa aaaacgagat
     7621 catacgcaat tacggttatt gttcttataa ttacaaaaac cagactgatt ttgatcaatc
     7681 tggtcctgaa aggattaaac aagcgattgc cagagaagct agtaaataca ttaacgatta
     7741 tgatacgctg tttatcaatt ctagctcgac tgcgttgaaa acactggatt ttcttcaatc
     7801 taaccatttg acagtcatta caaacaacgt gaaaatcaat tactctccgc acaaagataa
     7861 ttgtagttat attttaactg gtggagagat gcgctttcca aaagaagcac tagtgggcga
     7921 catcgcatta aatacgattt cttcagtcaa tgcagacatt tgtatcattg gctgtagcgg
     7981 agtcgatgta gtacatgggg tcacaacaaa gatattaaat gaatcaacga tcaacgaaat
     8041 gatggtcaac aagacaatca aatgtaagat tttagtcgct gatcatcgaa aaatcggact
     8101 gacatcaaag ttcaagattg ctgatatttc agtctttgat tatttgatca ctgataaatt
     8161 ttgttctgaa aaagtattag aggagatcag aaatacgggg ataaaaacca ttcaagtaga
     8221 ttcattttaa gaaccaggac gtaaatatgt aaataggatt atcaaaaaaa tgaataacaa
     8281 atttatcgat aaatgaaaaa cacgctacgc tcaaattgat aaaggaatcc caagatgtat
     8341 ccatccaatc gataccatca taatctattg cttcttttat aaccgttata tgttgcctaa
     8401 ataggaatca aacaatatcg aaaaaaggag gtatgacaaa agtcacacct ccttttttcg
     8461 aataaacggt attcaaatca gaacactgat ttcacaacca ctttatttca ggtcgttttc
     8521 aatcaccttt accgcgtaat catttccgtt gaaacgcgct attttttttg cgtccatata
     8581 tctttctaaa taatcatctt ctcctaattg cttatggcaa gaagcaattt cataaagcaa
     8641 gtattccaat gcataagtcg atttatgttt cttattatac tcaataccct tctcacataa
     8701 ttggattgct ttatcataat cctcgatttt acggtaatag gtggcgatat cataatatac
     8761 aatgcagacc atcagattat caaaatcagt ctcactcaat attttaagtg tacgctccac
     8821 ataaactttc gcaaaatcta attctccttt tgacaagtag atcattgcca cgtttgcagc
     8881 tgcttgtaag tgatggatat ctgtttcggt cgtttgttcc aacacttgat taaaataaaa
     8941 taatgctgca tctggttttt tcgcataaag atgatcaagc agtccttgat aaaataaaat
     9001 ttgatttaaa agatcagaat tacttacttt ttcctgatcg atcgtttgta aaattttata
     9061 agattcttgg tattttgata cagaattcaa tgattgaatc tcatttattt ttttcctgat
     9121 tttgtcttct tctgtttcta ctaatatatc ggagatctcg acattcagct ttttgcaaag
     9181 cgcacttaag atattgatgc ctggcgacat attcttgttt tcgatattgc tgacagttgc
     9241 ttgttcacaa atgccttctg ctaattgtac ttgtgttaac ccctttttct ttcggtattc
     9301 ttttagtaat gctccatcta ctttaatcaa aacaatttcc ttccctttaa aaaattattt
     9361 ctcctttata tcatacacaa aaaaaagttc taaacaacca aaaatgttta gaacagaata
     9421 aaaattttaa ttttaaaata atattcaaat taaacaactg gcgtatctgc tccttgattt
     9481 ctttttgtgt tatctgaata attatcgttt acagttataa cacatagtga aaattgtcat
     9541 ggcgagaaag tctaagaata tttttaaaca taactatttc tttcttacgt gtgtataatg
     9601 acgacagcac acctatcaat taatataatt attttatttt tttatgttct catgtttatt
     9661 tttaatgaca caac
//
LOCUS       DAZKNH010000096         9644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000096 DAZKNH010000000
VERSION     DAZKNH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.096"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="ACG8MG_002208"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="ACG8MG_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0376225.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="ACG8MG_002209"
     CDS             1995..4661
                     /locus_tag="ACG8MG_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0376226.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="ACG8MG_002210"
     CDS             5025..5777
                     /locus_tag="ACG8MG_002210"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0376227.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="ACG8MG_002211"
     CDS             complement(5799..6953)
                     /locus_tag="ACG8MG_002211"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFQ0376228.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="ACG8MG_002212"
     CDS             7138..9579
                     /locus_tag="ACG8MG_002212"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFQ0376229.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3111 a         1736 c         2066 g         2731 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agctgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tttt
//
LOCUS       DAZKNH010000097         9594 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000097 DAZKNH010000000
VERSION     DAZKNH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.097"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..641)
                     /locus_tag="ACG8MG_002213"
     CDS             complement(<1..641)
                     /locus_tag="ACG8MG_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFQ0376230.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQT"
     gene            complement(643..1407)
                     /locus_tag="ACG8MG_002214"
     CDS             complement(643..1407)
                     /locus_tag="ACG8MG_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFQ0376231.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1415..2092)
                     /locus_tag="ACG8MG_002215"
     CDS             complement(1415..2092)
                     /locus_tag="ACG8MG_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFQ0376232.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2129..3514)
                     /locus_tag="ACG8MG_002216"
     CDS             complement(2129..3514)
                     /locus_tag="ACG8MG_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFQ0376233.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3724..3808)
                     /gene="ffs"
                     /locus_tag="ACG8MG_002217"
     ncRNA           complement(3724..3808)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8MG_002217"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACG8MG_002218"
     CDS             complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACG8MG_002218"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFQ0376234.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4453..5310
                     /locus_tag="ACG8MG_002219"
     CDS             4453..5310
                     /locus_tag="ACG8MG_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0376235.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5391..5726)
                     /locus_tag="ACG8MG_002220"
     CDS             complement(5391..5726)
                     /locus_tag="ACG8MG_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376236.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5793..7163)
                     /locus_tag="ACG8MG_002221"
     CDS             complement(5793..7163)
                     /locus_tag="ACG8MG_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0376237.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7241..7777)
                     /locus_tag="ACG8MG_002222"
     CDS             complement(7241..7777)
                     /locus_tag="ACG8MG_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0376238.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8086..9567
                     /locus_tag="ACG8MG_002223"
     CDS             8086..9567
                     /locus_tag="ACG8MG_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0376239.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         2908 a         1970 c         1502 g         3214 t
ORIGIN      
        1 tttgtttgtt tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat
       61 aatacaaaaa ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt
      121 ggatcagtaa cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg
      181 ataaaaaagc gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg
      241 ccgatttgaa acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata
      301 aacaaaatac tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt
      361 ttcacttcat aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg
      421 atggttttga agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc
      481 tcttgatcta aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt
      541 ggtcgaaagt tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac
      601 aatccagcat attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc
      661 cagtgattgt ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt
      721 aaaacctgat aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt
      781 ttcatagtta tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc
      841 tttatccata atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt
      901 ctctacaaaa gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt
      961 cttatctggg ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac
     1021 tactttttca taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc
     1081 ttcccatgta aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata
     1141 gacttttttc ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta
     1201 gactgtctct ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc
     1261 tggtgactta gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc
     1321 ttccacaaat cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa
     1381 atcaattaaa tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa
     1441 atgctgtttt tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg
     1501 tttttgctga ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg
     1561 gtcctttttt ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag
     1621 ttgtcttttc ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc
     1681 aatattcgta aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat
     1741 tatccgcttg gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat
     1801 aatcatatcg ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt
     1861 cacgataatc ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta
     1921 acagtgtcac tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca
     1981 aagcagctgt cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag
     2041 caatttctgt tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac
     2101 cacctatctt ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat
     2161 ctcctaccac acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa
     2221 tcgttggaaa aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat
     2281 tcagatcgat tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc
     2341 cattatagac catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt
     2401 tacgtaaaaa gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac
     2461 ccaatgtgac atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat
     2521 tgacccgccc ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg
     2581 tctttcctcg ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga
     2641 tatctagact cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc
     2701 cacgttctag ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg
     2761 tagggatggc tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga
     2821 cttcaccgac atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt
     2881 gaccttctct gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt
     2941 cttttgcaat ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca
     3001 cttgtgaacg taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc
     3061 agccattcgg attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg
     3121 tctggtgttt cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat
     3181 ttttatcgaa taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca
     3241 actcttcttt ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa
     3301 cttcattcat cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac
     3361 gatccacaaa atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt
     3421 ctcgtttacg ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca
     3481 gatcattcgt atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt
     3541 atcataccat aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg
     3601 ccttgaagca aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga
     3661 acattcattt ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt
     3721 tcattataat cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac
     3781 gcttcacaag cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca
     3841 ttcgttgacc agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca
     3901 gtcaaaatca tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta
     3961 aaccgctcgt cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac
     4021 gcgccgaaat aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa
     4081 ggctctaagg taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag
     4141 gcttccttga ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg
     4201 ttatatcctc ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca
     4261 tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct
     4321 actgcatcaa gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta
     4381 ctttagcata tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa
     4441 gaggtgaaca agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa
     4501 aactctccag catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg
     4561 atcaaacaag attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta
     4621 atcaacgatc aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt
     4681 caagaaaaaa gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa
     4741 aaagaatggc aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat
     4801 acagacacag atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa
     4861 atcaacggta tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt
     4921 ggttctttcc attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt
     4981 tttttaacag agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg
     5041 gctttacatt actatacaaa agatactgta aaagatagta agttgatcaa aagttacata
     5101 gaactgatca aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc
     5161 aatgtcagct ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc
     5221 gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc
     5281 tatcttttat tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt
     5341 ttgtcacaac cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt
     5401 ttttcaacaa aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca
     5461 ccataaacag agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa
     5521 tccaagtcac tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat
     5581 ttgtcataga cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt
     5641 acttttcccg atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg
     5701 attgttactt ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata
     5761 gcatagacaa aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa
     5821 aagttcttta ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact
     5881 agcaaaaccc atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc
     5941 cattcctttt gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct
     6001 aatgcatagc taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt
     6061 acgactttta aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg
     6121 aacactaaaa gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt
     6181 ttttcctgct cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc
     6241 atctgatttt gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta
     6301 tgaggccata tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct
     6361 gaactaacgg attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc
     6421 cagctaatga tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt
     6481 cctttatttt ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag
     6541 tatagcggag attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct
     6601 tctcctgaac aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca
     6661 agttctgcgg gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt
     6721 tctgataaaa tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc
     6781 ttcatttgag cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact
     6841 ttttcaccgg gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta
     6901 tattcatacc cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca
     6961 ttgctgtagg gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct
     7021 gccggtttta gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa
     7081 aaaaaatcgg atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt
     7141 aacgttgtcg cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac
     7201 gaaaaataaa ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta
     7261 agatattttc caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt
     7321 caagaaaatc ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca
     7381 attcaagcga cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc
     7441 gcttaaatag cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat
     7501 ggatagaaaa aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt
     7561 cagctaagta gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc
     7621 tgattgattt gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact
     7681 ctcttgcagt aattgacttc attccatatt cttccacgta taccatactt ttgaacagca
     7741 aatattcttt actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc
     7801 tacccataaa taaataacat aaataattct aaaaatatta ttgataatta tcaaaacata
     7861 tttagttata tccattaaaa atcaattttt ccgccttaaa aacaagaata tcttgacaaa
     7921 ataccctagg aaataaacaa tagaataaac aaggtaacaa aaaaattaaa taacgttata
     7981 taaaaaagaa ggcgacactc tgtgagaata acacagactg aaatgacttt ccactcacag
     8041 tttttgtcta actatataca aaaacagaat aaaaaggagg tatttatgct aattgaagca
     8101 agaaaaaaag agttacttgt tctacaaatg gtcattcaaa aaaataattg ttctttacaa
     8161 gaattatcca atgacttaca tattccaaaa cggacaatca aagaactgat ccgaaaaata
     8221 aatgtatcac tcaagcaatt tttattgatt gatgaattta tttattctaa tcataaagga
     8281 gagatccaaa ccaaagaaac cgatcaaact aaaaagttag ctgttttttc taagttaaaa
     8341 ttatgctacc taaaagaatc caatcgtttt aactatttga tgttactaat caattatcca
     8401 caaactgctg taccaaaaaa atatttactt gaacaacttt atatatcgcc ctcctatctc
     8461 gaaaaactga caaaacagct caatcattcc ttaaaaaaat tccatttacg gatcctatct
     8521 gtaaaaaatt gctgtactct tgaaggaaat gagttatcta tccgtctcta tctttacttt
     8581 tttctttcag atacatttgg aggtaacgat tggccatttt cctcacttga attaaatgca
     8641 ctaaaaatga acagtacaga tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc
     8701 tacttgctgt taactttatt catattacga aacaaacaac aggcatattt tccaaaaact
     8761 tcttctgaaa tcactgaatt gatggactta ttgcagcata cacatgatta ttcagaatac
     8821 tttacttctt tttcagtgag tcgcccttgt gaagagacag aaacagagag agcctacttt
     8881 aattattttt tctattgttt ttttccaaat caaatctctt ttcgacaaaa acaggagatt
     8941 ggttttttat tttccacaag caaaaacctt atttgtcgac aagtcggtca attgatgact
     9001 tacttaactc aacagtttcc tctcaaccat ttagaaaata aaataccaat ttatttttat
     9061 tttcttgtgt tggcagttgt ttttttcgaa ctgttgggtg aatccagcag tagtttttct
     9121 acactttgct ttccagaagc cacgtccaat cattctagtt cttttataca ggaaaagaaa
     9181 ttaagagaaa aaatcgatac aattttttca aaaaatgggg aaagaaacca aatcagtcaa
     9241 taccttagtc aattgatcca acacttacta caaacagaaa cgaaagagca gttactcatc
     9301 catttgcaga taaccaaaca gtttacagga aattatcatc ttcaaagcca tttgcagcag
     9361 ttatttcgca aagatgctgt taaaattacc gcatattcag aacatgctga tttgatcatt
     9421 acagatcagc atgagaaaat ttcagatgaa acggagactt tctatctgga tacaacgcac
     9481 aatacagtgt tatttggcaa gttaataaaa gcggttcatg aactgtatat gaaaaaaggt
     9541 tatgagaaca aaacaaagag gatgtgacaa aattcttgtc acatcctctt attc
//
LOCUS       DAZKNH010000098         9431 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000098 DAZKNH010000000
VERSION     DAZKNH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.098"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..680
                     /locus_tag="ACG8MG_002224"
     CDS             <1..680
                     /locus_tag="ACG8MG_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0376240.1"
                     /translation="YFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHI
                     VQHIGRTFLNHRVKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRAS
                     FKDYLTESEIVDRLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETY
                     TTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRH
                     GKTFLTK"
     gene            800..1000
                     /locus_tag="ACG8MG_002225"
     CDS             800..1000
                     /locus_tag="ACG8MG_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0376241.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1076..1273
                     /locus_tag="ACG8MG_002226"
     CDS             1076..1273
                     /locus_tag="ACG8MG_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376242.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1570..3117)
                     /locus_tag="ACG8MG_002227"
     CDS             complement(1570..3117)
                     /locus_tag="ACG8MG_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFQ0376243.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3201..4004)
                     /locus_tag="ACG8MG_002228"
     CDS             complement(3201..4004)
                     /locus_tag="ACG8MG_002228"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0376244.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACG8MG_002229"
     CDS             complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACG8MG_002229"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0376245.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACG8MG_002230"
     CDS             complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACG8MG_002230"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0376246.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACG8MG_002231"
     CDS             complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACG8MG_002231"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFQ0376247.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6389..7318)
                     /locus_tag="ACG8MG_002232"
     CDS             complement(6389..7318)
                     /locus_tag="ACG8MG_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0376248.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7513..8195
                     /locus_tag="ACG8MG_002233"
                     /pseudo
     CDS             7513..8195
                     /locus_tag="ACG8MG_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
     gene            8356..9399
                     /locus_tag="ACG8MG_002234"
     CDS             8356..9399
                     /locus_tag="ACG8MG_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0376249.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2893 a         1933 c         1869 g         2736 t
ORIGIN      
        1 gctatttttc tcggttttca ttagcgactc gtgaagccgt tcaatggatt gttattgata
       61 tgtatgcgcc ttatgtttca ttggtgaaaa agcttttccc gaatgctcag ttgatcatcg
      121 accgtttcca catcgtccaa catattggca gaaccttctt gaatcatcga gtgaaagaaa
      181 ccacggcacg gcttaaagga acttcccact ctcaacgagg gttagggaaa aaattgaaac
      241 gctattggaa actcctacaa aaagaagaag caaagttaaa ctacgaaaaa agggtttggc
      301 gtgccagttt taaagattat ttgactgaaa gtgagattgt cgatcgactg ttatctgctt
      361 gtcccaacct gcgccaaggc tatcagttat accaagacat tctttatgcg gtaaagaaaa
      421 gagatcagtc attatttgag gactgtttaa acagagaagt cagcggtctt cctgaaacct
      481 atacaaccac tttacgaacg tttaaaaagt ttctgccaca gatccaaaat gcgcttcact
      541 acagctattc aaatggacca ttggaatgtt taaacaacca catcaaagta ttaaaacgca
      601 atgcgtacgg ctttcgcagc ttttacaatt tcaaactacg gatcatgatt cgtcatggca
      661 aaacattttt aaccaaataa acagaacaga aagagagttt cttctttctg ttctgtccaa
      721 attattctaa gatttctctt taccaacatc atttgacaaa gagcctgtat ttattactgt
      781 cgaggacaat ggcatcggca tgattgaaga ggaacaaaaa aacatttggg atcgctatta
      841 taaagccgat ccgtcaagta aaaatacaaa gataggcgag tcaggtcttg gattatcaat
      901 cgtgaaggaa ctagtgcgcc tgcaccacgg ttcaattgaa gtgaccagcc gaaaaaatga
      961 aggaacgacc ttcctgatac aatttccaaa agaaaaataa aattaccagt aaaaaaccgt
     1021 ggagagatag ctgctattga tttttgctat gtcttttctt tgagaggtat ccggtatggt
     1081 ggagtgtctg aaaaataaaa aaatggaggc caattttgtg aaagctgtta tttctggaag
     1141 tattgttttc caagaagaat atcgagatat ggaaaaagat ttcagtaaaa aaggcattga
     1201 attaattgat tatcctcgct catgtccgat cctagacaaa gagtatccag aaatcttgac
     1261 gacttttttt taaatatcgc agcagcggat ttattttttg tatttaatca agatcggaaa
     1321 ggggtattag gctatattgg tgccgcaact ttttcagaaa tgaagtattg tctgatgcaa
     1381 aatcttattc atggaaaaag gatcaaaata gtcttattta aggaaccatc agaggaaaat
     1441 tttttgtttt gatgaggtag atcgctggct aaagaatggc tggatggaaa tcagacagcc
     1501 taacaatttg gcataacaaa ggtgtgggca aaaaactgct cacacctttt tgaatttttt
     1561 atttatatac tacaaaatca cttgaaaatt atgattcact tttgcttcga tgcgtgggtg
     1621 aatattcaaa tagtcggcaa ttaattcggt catgtcgatt tggatttctc ggacaatttt
     1681 ctccgcacca aacatcgcat aatttcctcc gccgattgcc cgatattgat ttgtcaccac
     1741 ttccagcatt tgttgcggaa gaacggggtg acctttaaaa cgaagttcgg taatccgttg
     1801 tccaacaggc tgtttcatat caatagtgta ctgaatccct tcatacatgt cataattgta
     1861 gtactggggc ttaggctgaa taaaggctgg attgaatacg atttggttat tttctacagc
     1921 taaataattt gcggtctgct ccaacgccgc tctcaaatct gcaccattta tttgaatcac
     1981 tgccagagta ttcggataaa tatagttggt gatgacgtcc cgcatcgtga tcgtctcacc
     2041 aaagcctttt ccttcattat tgaataacgc agttccagaa atctctgagc cgctggcaaa
     2101 taattgtaca cgattaataa actctacata gggatgttcc tcaagacgag cctgcatcgg
     2161 atcattaata cgcatatttc cgttcacttt acctaagggt tgatccaacc atttctctag
     2221 tttttcttct accggtacga tcgctgcaag aatttctgga tcaggctctg atttttcaac
     2281 tgagtgtaaa gcaacctggt gatctgtaac aaccacttgc ccctcttttt tctcgacggt
     2341 aagacgaatt tcaccgatga actcgccacg atagcctggt tgaacaacgg gaactccatg
     2401 aaccgtttca gcaattttcc ggtgctgatg accggtgaaa agggcgtcaa tgccttcaac
     2461 ctgctgcaat agctcgtagc cttcattctc tccagttagc gcttctgtgt cctcaccggt
     2521 taacaagtct ttttcaaatc cgccgtggta actaacgatt accaagtctg cttgtttcct
     2581 taatagagga acatattctt ttgctgtttc gacgatactc ttaaacgtaa ttcctgctaa
     2641 ggtttctggc ttttcccagt gaggaatgta ttgtgtcgtt aatcccaaga tagcaatttt
     2701 tacacccacc ttttcgataa tcttataggc gtttccaaag tatggttggt catttttgtt
     2761 caccatattg gctcctaata taggcgcttg ataattctta atcgcttcct cgagataatc
     2821 cacaccatag ttaaactcat gatttcccaa cacttgaaca tcatagctca tttggttaat
     2881 caagtctgta agctctttgg ctgaaccata ttctttagca agataataac tcaaaggaga
     2941 cccttggatg aaatcaccat tttcgatcgt aattaccggt ccatctgctt ttgcccgtaa
     3001 ttccttcagt ttcgacgata ctttagccgt tcctaaggcc agatcggttc tgccggaata
     3061 gtctgttggt gaaacatagc catgcatatc acttgtctct aaaaacgtaa tgttcattct
     3121 gcgtgctcct ttagctgact caaaatagaa aaaccgaagc gtattcagct gttcggtctc
     3181 tttttttgat ttataatgcc ttaatccttc atttgaagtg tttttaaaat gttgcctgga
     3241 ataaagtttt ctgttgtctg gcgaacaatc cttccttgat cgagatgata aaggttatac
     3301 ccatgacgtt cttcaaagcg tacgaagcca tcgccttcat cttcaccaac aaaggacata
     3361 ctcgatacag taaaataggg gatcccatta atttcatcag taaatgtgtg atgggtatga
     3421 ccgtttagga tacagcgaat atcgtaaggt gcgattagct tcaagaaacg ttcggtacca
     3481 ggccattttg gtatactgct ttggtgagac agtagatgat gatgggtgac caagaaaatc
     3541 ggttggtctt tttcttgctc taaggctttt ttcagccatt gaaattgctg atcgtccact
     3601 ataccatctg cgtatccata gcttgaatta tcaaaggaaa tcactgtaaa ctcaggatat
     3661 ttgactatct ggttgtacgg ggcagaagag gcggcttcat cgcaccagcc tacccaaaaa
     3721 tgttctttga tatcgtgatt gcctagagta acaagaatcg tcgtttctcc tagcgcattt
     3781 tccagccaag tcttcaagta tcgataatcg tctatttcgc catcttcggt taaatctccg
     3841 ctgataatca caaggtccaa gggtgtttgc tgcaggagat gatgaatgca ttgctccaga
     3901 gggataaggg gattctgcat cgtagccagc attcccttat agccttcttc acaagcctca
     3961 tactctctgc gaaagtgtat atctgatata tgcaatatat tcattcttat ctcaatctct
     4021 ttcttataat tccggttatt tgatctaaaa taaacacggt tacgactacg ccaatcaaaa
     4081 taatactcac tttgctccaa gaccgagttt gaatcgcgaa tatcaatgga gcaccgattc
     4141 cgccagcgcc aactaagcct aaggtagccg cactacgaac cgctatttca aaatgattta
     4201 atgctaaaga acaataaatc ggcatcaaat aggggatgcg aacaaagaaa atcgtttgcc
     4261 aaaaatttgc tcctaccgca tgcatttctt caacaagtcg ctcatccatc gcttccattt
     4321 cttctgtaaa aagtttgcca agcattccga tctgatggac gccaatcgcc ataacaccag
     4381 caaaggggcc aggtccgacg actttcacaa aaataattgc aaaaactagt tcaggaaaag
     4441 ctcgtaaaac attacagata agcttgccaa tttttgttac ccatggatgt gcttgccaga
     4501 ggttaaccgc actgatcaag gtaatgggaa tcgctaatac cgtagcgatc attgtcccta
     4561 aacaggcgat tccaatggtt aataatagca aactaatcaa atcctcgccg ctgccatcat
     4621 atagaaactg ccaatcaggc tgacctaacc ctctcaaaac gtccattccc atggaaggag
     4681 agaaggtgct taatccagag taatccagag tagcggccga caaaataacg agaatcacta
     4741 aagcaactaa tgataaaagg gttcttctgt tcatttcacg aagcatcata acagggacct
     4801 cctgacacgt tcacttaatt tatcaacgat cgcgacagtt actaggatcg ttagaataat
     4861 gattgaaact cgttcataat tcaaaagcgc aagtgaggaa tttagaataa cgccaatacc
     4921 accagctccg acatatccaa ggacagtaga agcacgaaca ttcacctcaa acgcgtaaaa
     4981 agcatagctc gcaaactgac tcaaaacttg tggagcgatc gaccaaatag cgatttgcat
     5041 tttatttgct ccaacagccg cagcggcttc aatcgggcca aagtcaatgg tttcaattgc
     5101 ttgaaacatt aattgtgaca ccataccaaa agtaaagaca gcgatagtca acacgccagt
     5161 cgcttcacca attccaaaca cagcaacaaa aagcgcggct aaaagcaggg tagggatggt
     5221 ccgtactaaa cctaaaagaa aacgaaagat ggtagaaaga agacggtttt ctgtcactac
     5281 agtggtcgct aaaaaactaa cagggatcgc taaaattaag ccaatgaccg ttccaagcaa
     5341 agacatttga agtgttttca ccataggctc gactaattta ggtaaatagc taaagtctgg
     5401 atgaatgaag cgtgataaaa agacagtcat ttgattggag ttttgaaaaa cctgagaaaa
     5461 atcagctgac gtgatccagg cacttccttg aaaacaaaca attaagaaca gaagaataag
     5521 caggtgttta taccatttaa agtgaacggt ttctttcaag ctcatcgatt tcgcctgcct
     5581 tttcttcaaa gattgcattg ccataaattt gctctaagcg ttcatcggtt aagtcgtctg
     5641 gacgaccatc aaagacaacc tgagcagctt ttaacgcgag cacacgcgta gaaaactcac
     5701 gagccagagg gactgaatgc agattgacaa tgactgtttt atccatcgtt tgattgatat
     5761 tttttaggtc catcataacc ttttgagtcg tgattggatc taatgatgca acgggttcat
     5821 ccgctaaaat aatgggggct tgctgcacga gtgctcgcgc aatggatact cgctgttgtt
     5881 gcccaccact gagctcatca ctgcgtgtct gcaatttatc agacaaacca actttttcca
     5941 gtgcttcctt tgtctgctgg tagtcttcct tcgtaaaaag acctaaaata cttttccaag
     6001 tggagtaata gcccaaacgt ccagatagca catttttctg aaccgtacta cgctttacca
     6061 gattgaagct ttgcgagatt agaccaatct ggcggcgaat ctgtcgcagc tcttttttat
     6121 tcgcttttgt aatagaggtt ccattaatgc gaatatctcc tttggtggtt tctgccaagc
     6181 gattgatact gcgcagcaga gtgctctttc cagcaccact caaccctatg atggaaacaa
     6241 attctccatc ttcaattgtt aagtcaatat ccttcaagcc aacaacgcca ttgggataga
     6301 ccttcgaaac attgtcaaaa ataatcatta tccttctcca tttttttaag tgtaagcaaa
     6361 aaaaagcttg ctgacactta ctgatgtttt attctgctgc ttttttcgtg tagtctctga
     6421 tcgtgtcata ggcagaatca gcagcttctt catagccctg atgcccccac aaagccattg
     6481 cttctttgcc atctttatca tccttcatat ttaagaaggt ctctttaatt ttcgctttta
     6541 aatcttttga catgtcaggc tgtacagcga ttgcgtcatt cggaatatcg ccttttgtca
     6601 ggtacaggac ttttaaatcc ttaaataaat cattatctgg gaaattcgaa gcaaaaacat
     6661 ttcgcgcgcc ttcaaaaaca aacgccgcat ccatttgctt gttcaacact gcggtaatct
     6721 cactcggaat atcattcaca gtggtcatct ttgtatctgc agtaggatca atcccagcat
     6781 ctttcaattc agcgacagga tagatgtagc cgctagcaga gttaggactt aaggttgcga
     6841 tattttttcc ttttaaatca gttaacttat tcaatccgct atcggcacga accaatactt
     6901 ctcctttaaa ggtgttcgta agtttgtcct ttaatggcaa gccagtttct tgatcataat
     6961 cgccgagctg agaagtcagg attgcatctg cagctccagc atcctttgct tgaacatagg
     7021 ctgcaggcgg cataattccg acatcgactt gaccagaaga catcgcttcg acgatcgttg
     7081 aataatcagt agctaacgtc acttctacct cacgatctaa ctttttcgat aaatacttag
     7141 caaatggttt tgcttttgcc tccatactac catcattgtt tgttggaacg aattgaacaa
     7201 ctaatttttc tttgctctct ttttctgagc tgctacttgt attcccagaa ccgcaggccg
     7261 ccaaagtggt caataacatg actccgatga acccttttgc taactgtttg aactgcattt
     7321 ttctcctcct gttggatata atttctaata cgtctaaatc atagcaggga atcaaaatca
     7381 atgaagtgac gcacgtccta ttttacacaa cttttacata caatttacaa acaattataa
     7441 ataattattc gtattgagcc gaaaaacaat ctaagctata ataaagaaaa acttcttaga
     7501 ggtgacgatt tcatgaaaaa aatccatgat acgaagcaat taactgctta cgttgagaag
     7561 cacaacctgc aggattatat ggattcagat atattggcaa tggcttcttt actttttttg
     7621 aaaaggaaga gcacctcatt caaacgggaa aaacctcaga ttatttgtat tttttagtgg
     7681 atgggacggt aatggtgtac tcgtattctt ctgatacaca aaatatttgc atcgattacg
     7741 caaagcccgc tacaccactc ggcgaagcct cttcattgtg ggaattatcg ccgaaaagca
     7801 gcgttaaagc agtgactaag tgcatctgta tcagcattcc gctaaatcaa tatcgaacac
     7861 agcttcaaaa agatgttcgc tttttgcaga atatttgcca gttactaagt tatcgtttaa
     7921 attccgggat aaatttggcg aactcactga cagaacctgt tgaaactcgt ttagcaaagt
     7981 ttattttgac ccatcacgaa aacgaacgtt tctcatttcg gttaaccacc tgcgcctcaa
     8041 tcttaaatgt cagttatcga catcttttac gaacaatcac gaattttcgt gaggagaata
     8101 ttatcgaaaa gaaaaagaat tattacttga ttcatgatat cgaagcttta gaggacttag
     8161 ctgagaatat aagtagtcca gtaaaagcag aataacagct ataaaaaaac agcagtcaga
     8221 agtgattgct ggttttttga tagtggagaa attgggaaac ggaaaatctt cgttgaagag
     8281 tagtgttcta tatattggta tgctatactt ttaaaagttc aatgtgtcaa gacacataaa
     8341 aaggggagaa aaattttgaa gaaaatactt gtttcattgt tgtttttggt gtcattagta
     8401 acgttagtcg gctgtagtca gtctcagggg gagaagcaaa gtgaaaaaga gaaaagtaaa
     8461 atcgctttgg ttcttggatt tggcggggtt gatgaccgct ccttcaatca agctggctgg
     8521 gaaggattag aggcttgggg aaaagagaaa aagctgacag caaaaaaaga ttttacgtat
     8581 atagaaacga acaatccaga ggataacagt accaatatcg atcaggcaat cagaaacggc
     8641 tttaccacca ttattagctt gagctacttg caagcagagg aactggaaaa tgctgctaaa
     8701 gataatccaa agttgaaatt tgccttggta gatggtgaga ttactgggaa aaagaatatc
     8761 tcatcagcaa cctttaaaga tcaagaaagt gcgtatttag caggactagc tgccgcatat
     8821 acgacaaaaa gtgatcaagt aggctttatt ggtggaatgg aagggcccgt gatccaacgt
     8881 ttccaaagcg gatttgaagc tggcgttaag gcaggggcca cagcactgaa caaggaaatt
     8941 actgttaacg tacaatatgc tgcatcctat gattcgctag ataaaggaaa agctattgct
     9001 gcttctatgt acaaagaggg agtcgatatt ctttatcagg cggcaggaag tactggagct
     9061 ggggtgttcc aagaagcaaa agcccgcaat gaaaacgact atagcagaaa agtatgggtg
     9121 attggtgtag atcgcgatca agcggatgaa ggggattata aaatggagaa cggcgagaca
     9181 gggaatttca cgttgacttc tacaaagaaa aatgtcggtg cagaagtcaa agaatttgca
     9241 gaaacttctc ataacgataa atttcctggc ggcaagcatt tgacctttgg tttgaaagaa
     9301 gaaggggtag atctgacaaa aggacaatta tcagaggaag cctatcaggc agtttcagac
     9361 gcaaaagaga aaatcatcag tggtgatttc agactgtaga caaagtccat tttatggatg
     9421 atgtctacag t
//
LOCUS       DAZKNH010000099         9354 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000099 DAZKNH010000000
VERSION     DAZKNH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.099"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..264
                     /locus_tag="ACG8MG_002235"
                     /pseudo
     CDS             1..264
                     /locus_tag="ACG8MG_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 32"
     gene            1071..1244
                     /locus_tag="ACG8MG_002236"
     CDS             1071..1244
                     /locus_tag="ACG8MG_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376250.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYN
                     SPNEELFVKETDN"
     gene            1698..1913
                     /locus_tag="ACG8MG_002237"
     CDS             1698..1913
                     /locus_tag="ACG8MG_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0376251.1"
                     /translation="MKKKFVKVLVCCSTIGIGFTVLGTNTDAATYYGNGVYCNKQKCW
                     VDWNKASKEIGKIIVNGWVQHGPWAPR"
     gene            1930..2214
                     /locus_tag="ACG8MG_002238"
     CDS             1930..2214
                     /locus_tag="ACG8MG_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0376252.1"
                     /translation="MDKQQELLNLLSKAYNDPKINEYEGLKDKLFECASRLTNNEVNI
                     GEVCYKLSTIISKYLVTHNFKITESIIELQNFVTKESQKYRGWASIGIWS"
     gene            2187..2345
                     /locus_tag="ACG8MG_002239"
     CDS             2187..2345
                     /locus_tag="ACG8MG_002239"
                     /inference="COORDINATES: protein motif:HMM:NF033385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LsbB family leaderless bacteriocin"
                     /protein_id="HFQ0376253.1"
                     /translation="MGVYRYLELNNCFFIRVYSLWGHWISVQKLTRYEIAWFKNKHGY
                     YPWEIPRC"
     gene            2940..3320
                     /gene="mobC"
                     /locus_tag="ACG8MG_002240"
     CDS             2940..3320
                     /gene="mobC"
                     /locus_tag="ACG8MG_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFQ0376254.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            3302..4216
                     /locus_tag="ACG8MG_002241"
     CDS             3302..4216
                     /locus_tag="ACG8MG_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0376255.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGKEYAEKIAPEYQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLDKCYDINDEICLTHGLSITERGKKEEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYDQFCDSLKTFGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            4176..4454
                     /locus_tag="ACG8MG_002242"
     CDS             4176..4454
                     /locus_tag="ACG8MG_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376256.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDL
                     QVKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
     gene            4456..5145
                     /locus_tag="ACG8MG_002243"
     CDS             4456..5145
                     /locus_tag="ACG8MG_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376257.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKILQTRYSLKGSKIRFKRFFLL
                     GCIFSLIIVSSGLLGVLEGNKRVSRSILLGNVTQKYESARNKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYEQTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNIQSPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            5999..6931
                     /locus_tag="ACG8MG_002244"
     CDS             5999..6931
                     /locus_tag="ACG8MG_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0376258.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEAD
                     FKKQQQTEKEEAEKQSISNELKQDLLENLQNLFD"
     gene            6948..7418
                     /locus_tag="ACG8MG_002245"
     CDS             6948..7418
                     /locus_tag="ACG8MG_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0376259.1"
                     /translation="MTNKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTL
                     VVSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLD
                     QQQQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
     gene            7502..7954
                     /locus_tag="ACG8MG_002246"
     CDS             7502..7954
                     /locus_tag="ACG8MG_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0376260.1"
                     /translation="MTISKVIKQKRIEKQLTQEDIAEMLLVSKKTISNWENGRTIPDT
                     ENLIQFAKLFDLSLDNILLGEEKIMVKNHTKTLILLFHFVIFLSLVFTLIQSGFFYFS
                     HSIATWLLIFFLSLLLIINTLIIIFNKHRNLFLHSIFPIVLLIFLYFI"
     gene            8081..9262
                     /locus_tag="ACG8MG_002247"
     CDS             8081..9262
                     /locus_tag="ACG8MG_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0376261.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKI
                     TKELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVL
                     TGAKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIAT
                     LPIGILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISD
                     TGRDFLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNI
                     HVPVRESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVA
                     MIFFHVFENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQ
                     K"
BASE COUNT         3468 a         1451 c         1592 g         2843 t
ORIGIN      
        1 aaaaaaaaat tattggtagc tagtttgtgt ttgtttagca gtttattagc tttcacccct
       61 tctgtttcat tttctcaaaa tggtggagtg gttgaagctg ctgctcaaag aggataaatc
      121 tacaaaaaat atccaaaagg tgctaaagtg ccaaataaag ttaaaatgtt agtaaatatt
      181 agaggtaaac aaactatgcg tacttgttac cttatgagtt ggactgctag tagtagaaca
      241 gccaaatatt attactacat ttagtaaaaa caaaacattc tttctataca ataaaatagc
      301 gtatagagag gatgttttta atttgaaaag gagtggttat ctttatgaaa aatagatcat
      361 ttgctatatt agtattatcc gtaattttat tagatatatt gttaataaca ttagctttaa
      421 aaactttaaa tttagtaaca agttttgtca ttataccagt aattatagga atgtctattt
      481 atttcatcaa aaaaaataaa caattttttt gaatgaaatt acgggcgaaa ataatcactc
      541 aaaattatta ataaaaacga atcaactact ttttctgaag aaacatacaa ccgtaaacaa
      601 caattgttgt ttacggttgt aaattatcaa actatatggt gacttgttgc ttaaaatagg
      661 ttgaaatttc gtttattttt tttgagaaat tttacccacg tactacccat gtacctatcc
      721 acgtattacc catgtacccc cccatgacaa atgggcggag gggcttgcgg cgcttggtat
      781 taaaggcatt ctccgcctcc caggtttctg accattttct ctcaacgtgg taaaaacccc
      841 acttatgaga gaaaatggtc agaaacctaa acgatcggca aagaaaacat accaagcgcc
      901 acaagcaaaa aaagttagag taacttccag aaaagaaaaa ttttttagaa caacttttga
      961 aaatttgatt ttgatttata tccgttcaat ttaaacattt gataaactga aggctagtat
     1021 ttaaaatgtg tatacaagtg gtagcgttaa aaaaagtgga ggtataacct atgttaaaaa
     1081 aaactgtgtt ggtactgggt tctatttctc taggaatatt aggttttagc cccatgctat
     1141 tatccgtaat tggaaaaaac aattccaaca gagaacttaa attaaattca aattattaca
     1201 acagtcctaa cgaagaattg tttgtaaaag aaacggataa ttaataaaaa agactgctct
     1261 tccgagcagc cagttaaaaa ataatgagtg tttattcttg aaaaagcaac gcgcttatga
     1321 agcgctacca aaaatttatc atattttatg aaaaataaca ccccagtaaa aaatgactgg
     1381 taaaaattca caaagaaacg gaaagaagca actgcagaaa atcaagcctt aaaaagagct
     1441 tttgacctag accaatcgaa aatcacaaag tgagcccctg tatcggttga tacaagggct
     1501 acttggcata gtatacatta tgtctactaa cctttaatat aacatatttt cctaatttat
     1561 tctaactata ccacccgaac aaaaatgtaa gcattatgcc tactaacacc tcattgacaa
     1621 acttatatct ttcatttcat tcttttaaaa aagaaggcgc tcttctatct atctttttat
     1681 ttcagagatg atcataaatg aaaaagaagt tcgttaaagt tttagtctgt tgtagtacga
     1741 ttggtattgg gtttacagta ttaggaacaa atacagacgc tgctacgtat tacggaaatg
     1801 gtgtatattg taataaacaa aaatgttggg tagattggaa taaagcttca aaagaaatag
     1861 gaaaaattat tgttaatggt tgggtgcaac atggaccttg ggctcctaga tagaaaggat
     1921 tagtttaaaa tggacaaaca acaagaatta ctgaatttac ttagtaaagc gtataatgat
     1981 cctaaaataa atgaatacga agggttaaaa gataagttat ttgaatgtgc aagcagatta
     2041 acaaataacg aagtaaatat cggtgaagtt tgttataaat taagtacaat tatcagtaaa
     2101 tatctagtaa cacataattt taaaataact gaatcaatta ttgaattgca aaattttgtg
     2161 actaaagaga gccaaaaata taggggatgg gcgtctatcg gtatttggag ttaaacaatt
     2221 gttttttcat tagggtgtat agtttatggg ggcactggat atccgtacaa aaattaactc
     2281 gatacgaaat agcctggttc aaaaataaac atggatacta tccatgggaa attcctagat
     2341 gctaatcatt ggcagtatat ttttaatagt ctttttgata gtatttattt ttataataaa
     2401 aatgatgaaa gaaaactcct aaaaagtaat attagctata tatttaatcg ttgtattaag
     2461 tattatatta tttttagtgg aaatgctagc aattataaaa tatattatgg tcctactact
     2521 atttagtatc taaaaataat cgttaggaga taaataaaat gaagctatct acaacagtaa
     2581 aaaaacaatt gatggtgttt tagaaatact atcacaaatt ctactttata ttttttgtca
     2641 ttaattatca ctaggttgct atgcaatatt tcaccgaagg tgaaacaagc ggaactttac
     2701 aaaaagtggc tagcaaatgc tagccttttt tgtatggtgg agcgtgggtt ttgaggggga
     2761 ttcaaggggg agcttgcgcc cctttgacaa gcggttattt gcaccaaata cggaggtatt
     2821 tggctagcaa atatagagct tgccaaaccc ttttggtgag ctttactcat tgtacctttt
     2881 taggtacaat gagtattgcg aacgatacgt ccgtaggacg tactgaaagg gtgggactta
     2941 tgagcgaaca aaatcaaaat ttggctagcg atcattctaa aaaatatacc tatcgatccg
     3001 agccaaaaca aatcagtttt cgagtgagcg aatccgaatt tgcaaagctg aagcagtcag
     3061 ctgaagcttt gcaaatgagt gtgccggctt tcgtgaaagc caaggcacaa aacgcgcgtc
     3121 tggtgacacc gaaagtggcg ccagacattg cccaggcaat cgcgcgcgat ttagccaaag
     3181 ctggagggaa tatcaatcaa attgccaagt ggtgcaatac acaccaacat gacgttgctt
     3241 ctggtgacgc tcaacgttta tccgaaaacc taaaaatcat gcaaaaggag ctacaaaaga
     3301 tatggcaaca attaaagtaa gccgaaccac ttcttgtagt cgtgcaatta attacgcaga
     3361 accacgagca accgtcaaaa caggaataaa ctgtgacatt gattatgcca aaagtgaaat
     3421 gaaacaaata cgcacgcttt atggaaaaga cgatcgtgta caagcacact tattgattca
     3481 gtcctttcga ccaggagaaa ttaccgctga aaaagccaac caactaggga aagaatacgc
     3541 tgaaaaaatc gcaccagaat accaaatcgc aatttacacg cacacagata aagaccatat
     3601 ccacaatcac attgtcatta attcggtcaa tttagaaaca ggaaaaaagt ttcaagcaca
     3661 tggtcaagca ttcctagata agtgttacga tatcaacgac gaaatttgtt taactcatgg
     3721 tttaagtatc actgaacgag gaaaaaaaga agaaaaacga accatgtctg aaatcaaatt
     3781 aaaagaaaaa aatgaaccgg tttggaaaga cgaaattcgg tttgcgattg atcaaaccat
     3841 gaaaaacaag aaaaccagaa cctatgacca attttgtgat tctctcaaaa cattcggcat
     3901 tcattgcttc aatcgcggga aaaatttcac ttacgaattg atccacaaaa agaaaaaagt
     3961 acgatccaac agactaggaa aggactacga aaaggagacg attttacatg agctggacag
     4021 acgagaacga acctataacc aacagcgaac caacgagcta cgaagagctc gagctagcat
     4081 tcgcgaaact gcagaagaag tacgaagaga acaacgaact cttacgcctg aacaaccgga
     4141 aattagaccg gctcgaagaa accatgaaca acacgttgaa agaggaacag accaccaaca
     4201 agaattatcg ctctaaaacg ttaaaagaca tagaaaacga aataaagaaa caacgtgctt
     4261 ttctcgttcc ttatacgcaa gaaaggatac aaactacgat tgatttacaa gttaaaaaac
     4321 tccatcaaga gctattagac cagcgaaata aggaacagaa aaaggtgctt attcaacgat
     4381 cattggattt tcttgccccc ttatgttgct tagtcctgac actgttcgca ttttggttag
     4441 tatggggagg ctaaaatggc attttattta ccctatctac tcatttttgt tagtatttca
     4501 ggaagtattt ggctcatcta taaaattctc caaacaagat attcattaaa aggctctaaa
     4561 attcgattta agcgattttt cttgcttggc tgtatatttt ccttgataat cgtttcaagc
     4621 ggcttattag gcgttttaga gggcaataag cgcgtttcta gaagtatttt attaggaaat
     4681 gtcactcaaa agtatgaatc agcacgtaat aaaaagaaaa aagagcaagc attagcacaa
     4741 aaaatggaag agtttaccac ttgttatgag gaaatgaacg atatttttgt gaatcaagaa
     4801 aaacgtttga cggataaaaa catggaaaaa ctcactcgtc tttatcaaaa cttgccagaa
     4861 aaacagcaaa aagaggttca agacaactat gagcaaacca aaaaagacgt gcaatatgta
     4921 aaagatacaa agatagaaga agcctgtagt gatctatttg gagacacaaa cccttggttt
     4981 gccagtgaag aagaaaaaaa agaaaaacaa caatctgtca catatgaacg ctacgaaaac
     5041 ctgtttcaac aagcaaccaa catacaaagt ccaacgaaaa aagaaacggc attaaattat
     5101 ctagaaagtg tcaaggaatg gttagatcag caacaacaaa attgaatttg attgaaactc
     5161 atgttaaact aaatatgcaa ataggtaatc ataagataca cgaaagccct agagaagtcg
     5221 gattctctag ggcttttttt gtgggctgat gtcgccttag ttgattttac gtcttgtttc
     5281 ggttattcag ccaataactg aataaagagt ttagaatctc aacaagaagg gcgcaaataa
     5341 agttggtaat cacaagatac acctccttcc agtcgcaaga ctgtcggata ggcgacaagt
     5401 tattataccc tacaaaatta aatctgtgtg gcgattttaa gtatctctta ttttagggtt
     5461 gttagggctt gccctgaccg cctgtagggc gctttaaatt ccagataaag gtttttatac
     5521 taagactgtc ttaaaacggc ttagacaagc tcttagagcc tcaaccctcc tttttgaaca
     5581 atggttttag caaaggagct ggaggcgact gcgcaggtat aatgtgagag cgtagcgatc
     5641 acaccttttt ggcgaccgta gggagcgagg ttttaggtta gcagtttaga aaagttcgcg
     5701 aacctgttta gagaaaaaag aagtggtttt ttaggcggga aattttgtct ccctctttta
     5761 taatcttttt ttaaacctct ttttaaacct ctttaccgtc ggctgtgtcc ttactccccc
     5821 aagggataca aaattttata ggcatgaaat tatctgttta taggcatgaa attatctgtt
     5881 tataggcatg aaattatctg tttataggca tgaaattatc tgttttaggt attgttttta
     5941 gacataatat gtcctaaaat tatgtcaata aaatcattta gactggagta acaaataaat
     6001 ggcaaatgaa atcgtaaagt atgaaccaga attaaatacc attcctttaa gaaaatttac
     6061 cccaatagaa atgaacttgt ttttttctat tgtctctaga atgcgcgata aaggtaatca
     6121 aaaaatacgc ttttatttca gccaacttaa agatttaagt gcgtataagc agactgcagg
     6181 agaagtcttt ataaaagatt tggcaagaac atacgatcat ttgatggatt tacgtttcgg
     6241 aagaaaaagc aatggtggat taaccataga acgatttaca atgtttactg attttgaaat
     6301 tcatggagaa gtcgataacc cttatgtaga tataaaagtc cacgaaaaag ctttgccttt
     6361 acttaataat ctagaaagct gggtacgttt ctctttaaca gaatttcgag atttaaatag
     6421 cagttatgct aaaacaatgt ttagattgct aaaacaatac cgaactcaag gatgggctga
     6481 attttctaag gaagattttt tagagctatt agatattcct aaaagctaca gacaaacaaa
     6541 tataaatcag tttgtactca aaccaataaa agaagaacta actccactat ttaaaggatt
     6601 aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttattggtt atcgttttac
     6661 ttggaaagct gaaatcaacc atgctgacga tttttcaaaa gggaagcaag aagatttacg
     6721 tataaaatta tttaatattg aacacaatgg agaattaaca caaaaagaaa aatggcgtgc
     6781 taaagatcga atactaaatc ttccgttagg tactcatgaa gctgatttta aaaaacaaca
     6841 acaaaccgaa aaagaagaag cggaaaaaca atcaatctca aacgagctta aacaagattt
     6901 attagaaaac ttacaaaact tattcgattg aagcataagg agtatttatg acaaataaaa
     6961 cgatcaaaga actcgcggaa gaatttcatg tttcaaaaca agcaattagg aaaaggctat
     7021 cagctgattt tagagaaaac tatgtgcaaa ctgttactag gaacggagtg aaaaccttag
     7081 ttgtcagcta caaaggatat ttgcttttaa aacaacattt tttaggtagt aacaccactg
     7141 gtaaccaagt agaaaccgtt gctagtaacg atgaaaactc gattattgtt tttttacatg
     7201 aacagctcca agaaaaagat actcaaataa aacaaatgca aaagttactc gaccagcaac
     7261 aacaactcac cttgcaggca aatcaacaga tcgaaaaact gcaagaacag ttacaattag
     7321 cttatacgga agaacctgaa gaagaggaat taacttctgt caataaagaa aacaacaatg
     7381 caataacaac agaaaaaaag tggtggaaat tttggtaaca attaccaact ttccataaag
     7441 aagaaatcaa tttctataga ctttatcttt ctaagaataa aaaatatgtt taggtgatca
     7501 aatgactata agtaaagtaa tcaaacaaaa aagaatagaa aaacaactaa ctcaagaaga
     7561 tatagctgaa atgcttttag tatctaaaaa aaccatttct aattgggaaa atggtcgaac
     7621 aataccagat acagaaaatt taattcagtt tgctaagtta tttgatttat cactagacaa
     7681 tattttgtta ggagaagaaa aaattatggt aaaaaatcat acaaaaacac taatactatt
     7741 atttcatttt gtaatttttt taagtttagt tttcacctta attcagtccg gattctttta
     7801 ttttagccat tcaattgcca cttggttatt aatatttttt ctaagtttac tattaataat
     7861 taatacttta attattatct ttaataaaca tagaaactta tttcttcaca gcatatttcc
     7921 aattgtttta ttaatttttc tatactttat ataaaaagat agaactattt agtaagataa
     7981 aaaagtagct aattgtaatt ttcgtctaaa actcaaaact agtaaaaata aatttattat
     8041 gttattttta tttataaatt atgaaaaagg acttttcgaa atgaaaaata atagaatcta
     8101 caaaaatcag ttgaattatg ggcttttatt gccagttttt ctgctatgta tcattggatt
     8161 actttcttta tatgttgctt tatatcacga ctcttacact aattcaaaaa tcacaaaaga
     8221 attaattaaa caagtattgt ggtatagtat tgggttcact tcagcaataa ctatttattt
     8281 ttttaatcct aaaattttgt ggaaattaac tcctatattt tacggattat gcatcataat
     8341 gttattactt ctcttgaaat tctataatcc acaattagca gtcttaactg gagcaaaaaa
     8401 ttggttttat attgctggtt tttcgtttca acctactgag ttagtcaaaa taggatacac
     8461 ccttatgcta gctaaaataa ccgtcaatta tcagcaaaaa tctattacta gtaatacgaa
     8521 agatgattgg atttatttac ttaaaacttt tattgctact cttccaatag gaatattatt
     8581 attttttcag aacgatctag gaacaatgtt agtcttcatt gttatttata tttttatttt
     8641 gtttacgtct aacataaata taaaaataat tgccccaata atcgccattg gtgttgtttt
     8701 atccttaata tttttatatt tggttatatc agatacagga agagatttct tattaaaaat
     8761 aggatttcac gactatcagt ttaaaagaat agactcatgg ttgaatccat ttttagatcc
     8821 aaatggatct agctatcagt tagcaaatag tttaattgct attggctctg gcggactttt
     8881 aggtactggt tttaatgtat caaacattca cgttcctgtt agagaatccg atatgatatt
     8941 ttctgtaatt ggagaaaatt ttggctttat aggaagttgt cttgtaatat tcctatactt
     9001 ttatttaatt tatcaaatgt ataaaacatg ttttaaaagc aataacttat ttttcaccta
     9061 tatcggtata ggcattgttg caatgatttt ttttcatgtt tttgaaaata taggtgcaag
     9121 tatcggatta ctacctctaa ctggtattcc actcccctat attagtcaag gtggttctgc
     9181 cattcttagt aattttttgg ggttgggatt tatgttgtgt tgtagtagat atactaacga
     9241 aaaaaaagga acacaaaaat gaatttaaaa taacacttta tgaatagaat gtctatttca
     9301 ataaaaatta tgatacctta aagttaatca ttaaaaaagg agtgtcataa tttg
//
LOCUS       DAZKNH010000100         9291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000100 DAZKNH010000000
VERSION     DAZKNH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.100"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..690)
                     /locus_tag="ACG8MG_002248"
     CDS             complement(22..690)
                     /locus_tag="ACG8MG_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0376262.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            824..1111
                     /locus_tag="ACG8MG_002249"
     CDS             824..1111
                     /locus_tag="ACG8MG_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376263.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1527..3065
                     /locus_tag="ACG8MG_002250"
     CDS             1527..3065
                     /locus_tag="ACG8MG_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFQ0376264.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3062..4027
                     /locus_tag="ACG8MG_002251"
     CDS             3062..4027
                     /locus_tag="ACG8MG_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0376265.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4014..5336
                     /locus_tag="ACG8MG_002252"
     CDS             4014..5336
                     /locus_tag="ACG8MG_002252"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0376266.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5399..5503
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5577..6320
                     /locus_tag="ACG8MG_002253"
     CDS             5577..6320
                     /locus_tag="ACG8MG_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0376267.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6335..7327
                     /locus_tag="ACG8MG_002254"
     CDS             6335..7327
                     /locus_tag="ACG8MG_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0376268.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7339..8076
                     /locus_tag="ACG8MG_002255"
     CDS             7339..8076
                     /locus_tag="ACG8MG_002255"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0376269.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8066..8788
                     /locus_tag="ACG8MG_002256"
     CDS             8066..8788
                     /locus_tag="ACG8MG_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0376270.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8872..>9291)
                     /locus_tag="ACG8MG_002257"
     CDS             complement(8872..>9291)
                     /locus_tag="ACG8MG_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0376271.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3042 a         1603 c         1965 g         2681 t
ORIGIN      
        1 taaagtagtg aaaaataaga atcagtgctc taactgttca agaggatcaa gtggttcaaa
       61 tgtttctgta gtctgttttg cttccaattg gggcaaaaca gatccaattt ccagatactt
      121 cactttattc aatgtataag atagaataga acagctaagt aatgtataca gaaaatgtgt
      181 gagatccaaa taaagcaagg agacaactaa aataccaaaa gtaatcaaga aaagactttt
      241 gccaatctta atatgaaaat atttttcaat ggcttgataa atgatatctg ttccgcctgt
      301 gcttccgccg aatcgaaaga cgagaccgca accaatgcca gaaagaatac ctgtcatcaa
      361 actattgatc agtagactat ctacagcaaa atgtatatga aggctctccc ataaatgtaa
      421 aaagaaaact agcgcaaatg ttccgacgat cgtccggaga aaagcgtgtt ttccaaaaag
      481 aaacaatgag ataagcaata aaggtaaatt aaaaagcagt gaagtgacag aaggagcgaa
      541 tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc ccaccatctg ccagcttaga
      601 tggaatattg attcctgtca cagctaatcc gtagattcct gcgcctaaaa tgattccgca
      661 caattctgta aataaattta attttttcat ttctagcctc ttttcttttg aaaattttca
      721 gcaacttctt tattatactg acttatactc ttacttcaag aggaaaatga aaaaaaatta
      781 tttttggctg tttcttttta tttatcaaat aaggagtgga aaaatgcgag cagcagactt
      841 attgacaatt cttacaaaat cacccaaaaa aacagagtat caaggagttt ttttggctca
      901 agaacaaaaa ctggtgcctg tgacaatggt ccagataaat aaagcaaatc aatttatctt
      961 tttatttgaa cacggcaaag caccacttgc tatgaaagac atctttcttt ttttgatgaa
     1021 gaaccgtgag aaagctgttt attatcagtc aaaagaagaa ctcattcctc tttatggagt
     1081 gaaagagata gaaaataaag tggtgatata agtaaaaata agaaaacaag acgtttatga
     1141 tgtcaaaaca accatttttt acatttttgg caaaaaagtg tcatgataca gtataatatt
     1201 cgttgaacag gccaataaag gtgttctccc ccacgagatt tctactttat tggctgtttc
     1261 tttttattaa aataaaagtt gtgtgtgtaa aaaagatata gctgattcca agtgacaatt
     1321 tcgtatagat ttttatggta ttcgtagtga aaaaacgaga accaaaaagt tgatccgact
     1381 ttgttgcttg gattcttttt ttgtcagcat atttagaggt atttttttct tttttttgtt
     1441 cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa aggaaagaaa tgtttatact
     1501 taaagaataa cagaaaggaa ggtcgcatga ataattttat acaaacatta agtgaaagaa
     1561 aaggcgagtt gctggaagct gcatttcaac atctgtccat ttctctcatt tctttgctga
     1621 ttgcagcact gattgctatt cctttagcga tttgggcagc caatcataaa aaaatggcag
     1681 aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc ttcacttgcc ttattaggtt
     1741 tacttatccc atttgtaggg atcggaactg ttccagcatt gattgcactt gttatttacg
     1801 ctttgcttcc gattttccaa aacacgtata ttggtctagc tgaaattgac ccgtctattg
     1861 aagaagcagc ggtagcattt ggtatgtcca gaatgagacg cttgctgaaa gtagagcttc
     1921 ccatcgcatt acctgtcatt atctcaggta tccgaactgc cttagtttta atcatcggta
     1981 cggctacttt ggcagcattg atcggagcag gtggattagg gacatttatt ttgttaggta
     2041 ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat cagttctgcc ttgcttgcaa
     2101 ttatattcag cggactgatt cgctggttgc agcataagaa acctcgatat tccatcatta
     2161 ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt atatcagtct gccttgttta
     2221 aaaatgaaac catcacgatt gctggaaaat taggagcgga acctgaaatc ctgatccaaa
     2281 tgtataaaga attgattgaa gacaacacaa aaacagaagt tgaattaaaa ccgaattttg
     2341 gcaagacgag ttttttgttc aatgcactgg atcaaggtca aatcgatatc tatcctgagt
     2401 tcacaggtac tgtcctagaa agcttggtga aggtacctga agcaatgaaa agtgctgatt
     2461 tgacaaaaga aaaaacatat gacgaagcag aagaattatt gaataagcaa ttcgatatga
     2521 cattattaaa accaatggct taccaaaata cgtatgcctt ggcagtcaag agatcttttg
     2581 cgaacgaaca tcaacttaaa aagatttccg atttggcagc tatctctgat cagatcaaag
     2641 cgggattcac actggaattt attgatcgtt cagatggata taaaggaatc caagaagcat
     2701 atggcttgaa tttttcaagt gtacaaagca tggagccaag tttgcgttac caagcaatca
     2761 ataatggaga tgtcaatatt gtggatgcct attccacaga tagccagctc aaggagtacg
     2821 atctagtgac tctagaagat gatcgagggt tattcccagc ttatcaaggc gcaccgctga
     2881 tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa agcactgaat aaattaagcg
     2941 gaaaaataac agaggatcaa atgattgata tgaattacca agtcaatgtc gaaaagaaaa
     3001 agccgacaga agtagcacga atcttcctga aaaatgaagg attagtaaag gaggaagatc
     3061 agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag tatgatggaa agtatgtcat
     3121 cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt gttttggttg gtccttccgg
     3181 tagtggaaaa acaactactt tgaaaatgat caatggatta tctaaaccat cagcaggaga
     3241 catctacttc aaaggaaaat cgttaaatga atataatctg cagaaaatgc ggtggaatat
     3301 gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg acagtcaagc aaaatattga
     3361 agtcatccct gaaatgttag gttgggaaaa gcagaaaaga gcagaccgtg tagatgaact
     3421 tcttcaaaaa gttggcttat ctcctgatat ttatcgagat cgtatgccta gagagttgtc
     3481 tggaggagaa cagcaacgga tcgggatcat ccgagctatt gctgcaagcc cagatgtgat
     3541 tttgatggac gagccattta gcgcattaga tccgatttca agaaactcat tacaagaatt
     3601 ggttttgtcc ttacacgaag aattgggcac gaccatcgtt ttcgttacgc acaatatgga
     3661 agaagcaatc aagctaggtg atcgtattgc atttatgaag gatggagaaa ttatccaatg
     3721 tgacacacca gagcagttat tgatgaatcc taaaaacgac tacgttcggc atttctttga
     3781 tgaaccgaaa cagacgaaag agtggcgagt agaagattta gtcgtcaatg gatatttctt
     3841 aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt gatacgccta tgaaagaagt
     3901 atattcgctt ctttcggtgt atccaagcat tgtgatagta gaaaagcaaa gaagtattgg
     3961 gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga gaggaggaag gaaatgaaaa
     4021 catatgatgc aatcatcatc ggttcaggtg tgagcggctt aagtgctgct tatggattga
     4081 aagaagcagg taaaacagta ctagtggtcg aagaagatct atggggcggg acttgtccta
     4141 accgtggatg tgatcctaaa aaagtcttgt taagtgcagt agaggcacgt aatcgggtaa
     4201 aacaattgag tggaaaaggt tttaacgaga tcccaactgc caactgggaa gaacttcaaa
     4261 aattcaaacg aacatttacg gatccagtcc cagaaagccg aaaaaaacaa ttagcagaag
     4321 ctgaaatcga tcatctttca ggaaccgctc gttttttgga tgattcatct attgaagtca
     4381 acgaagaagt cttccatgcg gattacttag ttttggcaac cgggcaaaga cctacgatct
     4441 taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga ctttttatca ttgcctgtac
     4501 tgcctaaaga aatcattttt atcggtggtg gttatattgc ttttgagcta gctacgattg
     4561 ccaatgctgc agggagcaaa gtaaccattg tccatcataa tcaacggcca ttaaaagaat
     4621 ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga agcatctggc attcagtttg
     4681 cttttggtgt tgaaacccaa aagatcattt cagaaggtac tcgctatcgt ttagtcggaa
     4741 aagagacgga attagtagca gatatgattt tttgtgcgac tgggagacag ccgaatacag
     4801 aatctttagc acttgagcaa gcgaatatcg tctttgataa acacggaatt gcagtcaatg
     4861 attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg agatattgtt tcaaggaaaa
     4921 ccccaaaatt gacaccagtc gccacttttg aaggaaatta tgtagctaag cggataacgg
     4981 atgcaacaag cgaacctatc aaatatccga ttatacctac gatcgtctac gccagtccta
     5041 aacttgctga agtaggagtt acaaaaagtc acgcctcttc gtcagatcaa gtggtagaga
     5101 tggatcttac cagttggttc acctatcatc gtgtcaatga accagttgct aaggctgaat
     5161 tgacctttga ccagcaaaat tatttgattg gtgctgcagt gattagcgag caagcagacg
     5221 aattgatcga tgatttgaca ctcgtcatca atcaaaaatt gactaaaaaa gagttggata
     5281 gctatatcat ggggtatccg acattggcaa gtgatctctc ctatttattg aaataatcag
     5341 catgataact aaagcgcttg ttgctttttc cttcttgtgc tacactttaa aaaatgaata
     5401 cctgtttctg gggatgtcgt agacgacatg agagtaagtt gtgaacttag acccattgaa
     5461 cctgtcagtt aatactggtg aagggagaaa catagcagac gcttgaagcg ggttactatg
     5521 tcgtgcccat ttcaagcgtt ttttgttatt catattagaa gaaaggaggt aacaaaatga
     5581 agaaaaatct ttatccagca gtcagtatgg cattcatttt aattatttgg cagattgttg
     5641 tcatagtttg gaaaatacct gcctttattt tacccggacc tgctgctgta ttttctgcat
     5701 tagcggcaga tgcacaagta ttactttccc attctgtcac gacattaaat gaagcagtgc
     5761 ttggtttggt aatagctagc gttctttctt ttattactgc cttggcaatg gattattgga
     5821 agtttttaga aatgtctttt tatccgttac tagtgatttc acaaatgttg ccaatcatgg
     5881 tgttagggcc attacttacc ctatggtttg gtttcggtat gcttccaaaa gtgatccttg
     5941 ttgtcttgat aagctatttt ccgatcgtag tttctttttc ggatacattg aggaaagtat
     6001 caaaagagca aatcatcttt ttgaaaacga tgggggcaga tacagggaag atttatcgaa
     6061 tatataaaat accagcgggt ttatccggct tcttttccgg tttaaaagtg gctgctactt
     6121 attgtgtcag cggagcagtc gttggagaat ggctaagcgc tcaagcaggc ttagggtatt
     6181 acatgatacg cgtaaaaaac agctaccaga tcgataaagt ttttgctgcg atcatttgtg
     6241 tcattctatt gagtttattg ctgaatggcg cttgttcatt attgaaaaaa gggtattatc
     6301 gtatgcttta tattttttaa gagaagggga tcaaatgaaa aaattatggc ttttattacc
     6361 gctactgttg cttttatccg cttgtggcac tgcaaaagaa acaagtagca aacaagaaat
     6421 aaaggatttg aaaaaagtga cacttgtttt ggattatgta ccgaatacta atcatactgg
     6481 catctaccta gcaaaagaaa aaggctatta taaagaggct ggtttaaatg tccaaatcat
     6541 cgaaccagga gataattcga caagtattgg tctagtaggt gcggacaaag cacaatttgg
     6601 tgtaagttat caagaagatg tcacttatgc tcacgcagat ggacagaata taccagtcaa
     6661 agcaattgct acagtgatca aacataatac ttctggattc gccaccctat cagacagtaa
     6721 catccattca ccaaaagatt ttgaaggaaa aacatatgca ggttggcaaa gtccaagtga
     6781 agaagctgtc ttaaaagctg tgatggaaaa agacggcggg gacttttcaa aactgacaat
     6841 ggtaggaagc aatggagaag ggccagaaag cttaggaaaa tcctcagata tccaatggta
     6901 ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt attgaactaa actatatccc
     6961 attgaaagaa ttagatgaac ggttagatta ctatactccg gtaattatta cgaatgatca
     7021 actgatcaaa agtgatccag aattggtaca gtcttttatg gatgccacga aaaaaggcta
     7081 tcaggaagct attaaggatc caaatgacag tgctaaactg ttgcaaaaat atgcaaaaga
     7141 aaatgatcgg acgtttttag aagaatcaca agcattcttg tcaaaaaatt atacggatga
     7201 tcctgagaat tggggattga tggaagaaaa agtttggcgc aattatacag cttttatgca
     7261 ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa ctattcacta atcagtttat
     7321 aaaataggag tgatagatat gtcaatccaa atcagccact tgaatgtagt cattgaaaac
     7381 ctgaaaatat tagaagacat ccatttcact atccctaaag atacctttac atcgattgtc
     7441 gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt taacaggagt ccagcctatg
     7501 acttctggaa cgatcaaagt agacgatcaa aaagtaactg gtctccatac agcatttagt
     7561 tatatgccgc aagaagatat gttacttcct tggctgagcg tctatcaaaa cgttacgtta
     7621 tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag taagggatta tctggatatc
     7681 tttgggttaa acggctatga acattttttg ccggaacaat tatctggcgg gatgaagcag
     7741 cgaacggcct tgttacgaac aatcatgaat ccttcttctt acttgcttct tgatgaacca
     7801 tttggagcgt tagatgctat gaccagaggg cagatgcaag actggatgtt gaagctgcct
     7861 aaaaaagcaa agcggactac tttacttgtg acgcatgata tcgaagaagc aatttattta
     7921 tcagacagga ttcttgtgct ttccgcccgt ccggcacatg tgattgctga gattcaagtg
     7981 cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat cagagttgaa gcaaacgatt
     8041 tatcagttat tggcaggtga attagatgtt aagtgatgct cattgtcatt tagatgggag
     8101 tcagagttta gcactccttc agcaggaaca cacgattttg acgatcatca attgtgattc
     8161 gccagaagaa tggaaggaaa acagacagct tgcagcaagt aagactcaag ccttaagtta
     8221 cggtatccat ccttggaaag cagatagcta tacgtttgaa caagttgagc cttttttgaa
     8281 aaaagcaagg atcatcggtg aaatagggtt agataatatt tggacgaatg taccgatgac
     8341 tacacaaaaa aaagtatttg agcgacagct tgcttttgca gccataaatg agaaacctgt
     8401 tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat tatatccaag cttaccctaa
     8461 tcactatttg atccattggt attcttcttt ggactatcaa caagactata ttgatttagg
     8521 ctgttatttc agcattggtc ttgatttgaa aaagaatcca gcagtctggc aactagctaa
     8581 agcagtccca atcgaccact tacttattga aacggatgga ctacatgcag cagagtgggc
     8641 tttagggggg attttttcag aaaaagatta tgtctcactg cttgaagaac agttaaagac
     8701 aatcgctgag atcaaagagc tatcagttga agaagttaaa aaaaatactt atcaaaactt
     8761 ggaacgtttc attagattag gtaaataaaa aagagatgtg acacaactag tgtcgcacct
     8821 ctttttggct ttctttgtat aaaagtaaac tgtttgatta ataaatgaat cttagtacca
     8881 accgtttgct tcccagaatg ctttcgccgc ttcccaagaa ccataacgtg aagtaacata
     8941 ttgttctgct actctttctt ggtttgcagc agagtagtca ccatttaagt aagaagaatc
     9001 taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg taagaaccgc ttgattcttt
     9061 ttgtgcgatc cattcttttg cagagcttgt gcttgctgga gtagcagcag tttctgttac
     9121 agcaggagca ggttgtgttt cagcaacagg cgctgcttgt ttttccgttt gaaccgtttc
     9181 ttgtacaaca ggttgttcag cttggactgg tgcttcttga acggctgctt gaggttcagc
     9241 agctgcttgt gcagagcctt ctgttgggat tgttaattgt tgtcctgagt a
//
LOCUS       DAZKNH010000101         9062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000101 DAZKNH010000000
VERSION     DAZKNH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.101"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACG8MG_002258"
     CDS             414..947
                     /locus_tag="ACG8MG_002258"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0376272.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1437..1961)
                     /locus_tag="ACG8MG_002259"
     CDS             complement(1437..1961)
                     /locus_tag="ACG8MG_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFQ0376273.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(1954..2427)
                     /gene="tsaE"
                     /locus_tag="ACG8MG_002260"
     CDS             complement(1954..2427)
                     /gene="tsaE"
                     /locus_tag="ACG8MG_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFQ0376274.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2550..3530)
                     /gene="pta"
                     /locus_tag="ACG8MG_002261"
     CDS             complement(2550..3530)
                     /gene="pta"
                     /locus_tag="ACG8MG_002261"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFQ0376275.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3683..4363)
                     /locus_tag="ACG8MG_002262"
     CDS             complement(3683..4363)
                     /locus_tag="ACG8MG_002262"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0376276.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4489..5358
                     /locus_tag="ACG8MG_002263"
     CDS             4489..5358
                     /locus_tag="ACG8MG_002263"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0376277.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5384..5899
                     /locus_tag="ACG8MG_002264"
     CDS             5384..5899
                     /locus_tag="ACG8MG_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFQ0376278.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(5946..6509)
                     /locus_tag="ACG8MG_002265"
     CDS             complement(5946..6509)
                     /locus_tag="ACG8MG_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0376279.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6564..6743)
                     /locus_tag="ACG8MG_002266"
     CDS             complement(6564..6743)
                     /locus_tag="ACG8MG_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376280.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(6820..7329)
                     /locus_tag="ACG8MG_002267"
     CDS             complement(6820..7329)
                     /locus_tag="ACG8MG_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376281.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7425..7805)
                     /locus_tag="ACG8MG_002268"
     CDS             complement(7425..7805)
                     /locus_tag="ACG8MG_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0376282.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            8135..8884
                     /gene="pnuC"
                     /locus_tag="ACG8MG_002269"
     CDS             8135..8884
                     /gene="pnuC"
                     /locus_tag="ACG8MG_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFQ0376283.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2626 a         1849 c         1621 g         2966 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttaccaagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca
     1261 aacggcaaaa tagttcctca tattttgccg ttttttgagc taataccgaa ggtcagctga
     1321 ttgaacaccg tttattcaaa aaaaatggac tgcgacacga cttttgtcac agtcccttcg
     1381 tgtgtttttc tgccttctca aaaggatgac agtttctcac tcttttccta tttcttctat
     1441 tcaatcaaat aactcatttt atatacatct aaaaattcac ctagatctgt tcttgcccct
     1501 ctaggcatga ccgcttcgat ttggaagccc atttttcggt aaaggtgtac ggctctttca
     1561 ttacgaactt gaacgtcaag ctccaatcga aaaagtacac ccatttcctt cgcccaactg
     1621 atgatttctt ctagcataag agtgcctagt cccattcccc aatattcctg cagaatactg
     1681 ataccgactt ctcccacgtg agacaaacga aattgactgt ctgccttgac agatgctgtt
     1741 ccaataatcg tcccttcata gattgcaagc agcaataaat tattattgct ttcgtatata
     1801 taatccaact cttcttctaa ggtttctggt ggtaacagga gttctgcttc atccaaaact
     1861 aaaaattctg tctccttatt aacttgtccc atcatcaaaa gaatgtcttt tgcatctcct
     1921 ggcactgctt cccgcaagat gatcccaact tcttcactca tgattttcag cccatttttc
     1981 tagaattcgc tgtttgaagg tttcactttg ctccccgtat gagattagtt tcacataccg
     2041 cttttctaaa tctgtatcac ttttttctaa aatcagttcc aaataatcct ccggcagtgc
     2101 ttcttctaat agatttcccc attcaacaac ggaaagccca tcaccttcaa aatattcatc
     2161 taaccctaaa tctgctccgc tagctgctac ccgataaata tccatatgat acaacggaat
     2221 ccgaccttga ctatattctc taataatcgt gtatgtcgga cttttgatca tttgcgttat
     2281 ccctaatcct tgggcaattc ctttcgtcaa cgttgtttta cccgcaccta gatcaccagt
     2341 caatatcaga ttgtcacctg gcattgcgga atgaccaatt gcctctgcga attgttctgt
     2401 cattgttaat cctgatagtt cgatcatttt tcatcttctc ccgctcttct ttctttttgc
     2461 cagtatatcg cgtaatgaag tttttttaaa gaaaaaatga actagtcaag aacagcagct
     2521 gctgtttttg gctagttcgc gtgtatttgt taatcaatca atgtttgtgc tgctgtgata
     2581 atcgataatt tatatacatc ttcttcattt gctccacgag acaaatcaga aaccggtttg
     2641 ttcaagcctt gtagaattgg tccgattgct tcaaagttac cgaaacgctg agcaatttta
     2701 tagccgatat ttcctgactg caattctgga aatacaaaga ctgttgcttg accagctact
     2761 tctgaatctg gcgctttcag ttgtgcaact gaaggaacat aagaagcatc aaattgcaat
     2821 tcgccgtcga tcatgtattg tggcgctagt tttttagcga ttttagttgc ttcaactact
     2881 ttgtctactt ctggcgattt cgctgagcct tttgtcgaga agctcatcat tgctactttt
     2941 ggttcgatat cgaacaattc tgctgttttt gcagaatcta cggcgatttc agccaattct
     3001 tgcgcagttg gattcacgtt gatcgcacaa tccgaaaaga catatttttc ttgatctctt
     3061 ccacgtacca tgatcatcgc tccgcttgtc cgactgatgc caggctttgt tttgatgatc
     3121 tgaagtgctg ggcgaacagt gtcgccagtc gaatggatcg ctccagagac cattccgtct
     3181 gcaatcccca tatacgtaag catcgtacca aagtaattca catcttttaa aatcttttct
     3241 gcttgttctt tggatgcctt tccatttcta cgttcaacaa aagtatccac catttcatcc
     3301 cacttatcgt aattatcagg gctgatgatc gtaaagctag atgttttgat tcctctggca
     3361 tgcgctgcat ttcttatttc ttcttcgttc cctactaaaa tgggttccac aagttcttct
     3421 gctttcaacc gggcagctgc gcctaaaata cgagcatcag tagcttccgg gaaaacgatt
     3481 ttgatcccac ggcgaacaat tttaaatctt aaactatcaa acaattccac aagatgacct
     3541 ccagacgttt ttatatgtat tatcatacac tattccagaa aaaaagaaca gtttatttta
     3601 tctgtatcag tcaaaaatgc tggaaaaatt aagtttttca catacaaagc gaactgcact
     3661 ataacaggaa agcgctgttt tattacactg tttcaggcag ttgccaatca atcggttctt
     3721 ctcctaacgc aaggagcgca tcattcgttt tcgagaaggg tttggaacca aaaaagccac
     3781 gacttgcaga taacggactg ggatgtggag atgttatgat gatatggcga ctcttatcga
     3841 tcattttgat tttttcttgt gccggttttc cccacaagat aaacacgatt ggtttttccc
     3901 gttcatttaa tttttcaatg atcgtgtctg ttaatctttc ccaacctttc ccgcgatgag
     3961 aatacgcttg cccttcccga acggtaagaa cggtgttgag taaaaggact ccctgtttag
     4021 cccatgacac tagatttcca tgttgaacag gtgcgattcc aagatcatca taaagctctt
     4081 tatagatatt tcttaacgat ggcgggatct ttacgccagg ttgcacagaa aaagataagc
     4141 catgagcttg attttctcca tgataaggat cttgtcctaa aatcaccact ttaacttctt
     4201 cataaggagt caattccaat gcttcataga tatgatacat atctggatgg atcttttggg
     4261 acgcatattc tttttttaaa aattctctta gatgcagata ataatctttt gaaaattctg
     4321 aggacaaaac ttcttgccaa ctgttatgaa tgatttcttt caaaaactcc acctcctaat
     4381 aaaaagaata actcacattt tcttcaatca caagggacaa tcttcaccct cagcacttga
     4441 ttaatatggt aaactaggaa caagaagcag aaaggaacga taatcattat gatcaaatta
     4501 attgcatcag atatggacgg cacattatta gatgcccata tgagtgtttc tcctgaaaat
     4561 gccgaggcga tacgttttgc aaatgatgcc ggtgttgagt tcatggtcgc tactggtcga
     4621 aactatcaag aagctcgcgc agctttagat gaggtaggaa tcgattgtgc gatgatcact
     4681 ttgaacgggg cacaggtatt tgataaagaa ggaaattcat tgtttaccgt gtcgattcca
     4741 aataatcaag caatctctgt tttagatatt ctagatgcta acggtattta ctatgaagta
     4801 gcaaccaacg atggtcttta ttctgaaaat caagcgaaac gaattgaaag ttttgcttcc
     4861 atggtggcta cccatttacc tcaccttacg tataaaatgg cgattgccat ggcctcagct
     4921 aagctggagt tattgcacat cacatacgtg gacagtattc gtgctatttt ggaagaaaag
     4981 aatctggaag ttttaaaaat tatttgtttt catacagaag ggccagctgt attaggtcca
     5041 gttggaaagc agatttctct tttaggcgat ctagccgtta cttcctctgg tcaaaacaat
     5101 atcgaagtga accataaaaa cgctcaaaaa ggaattgctg ttgcccatgt cgctcacgag
     5161 cgtgggattt ctttagaaga agtcatgacg attggcgata acttcaatga tgtcagcatg
     5221 ctgcaagtag caggagtcag tttcgcaatg ggaaatgcag aaatcgaagt aaaagattat
     5281 gccaaatata caacggacac aaaccttgaa tctggcgtag gaaaagccat attgcgcgcg
     5341 attgcagaag acttgtagtt ttatctagaa aagagggata tagatgtcag aatttttcat
     5401 tcaggaaaag cagcttggac gtgcaactcg gacaattgtc aaaaacgaaa aaggacattc
     5461 cctgtttttg atggttggtc gctggggaac aaagggcgat gcattgtctc tttatgccat
     5521 gaatggcgat ctcgttgctc acatcaagca gataagcctg acattcggta cgcgttttga
     5581 gctgtataaa gagttcgaaa aggtgggaac catgcagaaa atattcaatt ggcccggtga
     5641 cttttattat attcgtagac ttcactggac agtccaaggt gatatctata atcatcaata
     5701 tcaaatccat catttcaatc agcaaatcat gaaaatggat aaagcaacac ttttttcagg
     5761 agattactat gtattggatg tctcaaatga tgaagatgct ccagtttgta tttgtattgc
     5821 tgcggtttta gattactggc tctaccgtaa agacaaaagt cgcgataagc ctgtttctta
     5881 ccgacttaac ttaagttaaa aagatgtacc tgaaatgcgg aaatacgcat ttcaggtaca
     5941 tctttttaga tattcttttg catatgatca taaagatgac cactgatcat ccgctgaccg
     6001 acaactttga agtcatggcg ttcgtataat tttttagcct ttggattttt ttcatccaca
     6061 cttagaccga tgacggagcg attagctttc ttcgccaatt taggtaatgc ttctaataat
     6121 cgcgaaccga tgccttgtcc tctaaaatct tctgatacac tgatagagtc caaataccat
     6181 tcgttgggaa acgtttcttt atcaatgaac aaacgtgtag acgcatctag ctgatatttc
     6241 tctaagatag gtaccagtgg ctgatcgatg attgattctt cttcatctgt ataaccgtaa
     6301 gcaactcctg caactttacc gtctatttct tctacgatac ctcgtgaata actgtaacga
     6361 taatttggat cagtgattgc ttcttctaaa atctctaaag tcttttgttc ccctgtctgc
     6421 ttcaaaaatg gcagttccat atcttttaga atcactaaga tcaatggggc gatttctgga
     6481 gcatcttctt ttctcgcaaa acgaatcata gtttcctctc cttttctcca ttctaacatg
     6541 cagaaaaaga aatcgcaaaa tcgttatttt aaaaaatctt caatcttttc agtaatgaaa
     6601 taaccatgag taacgattct tgccgcttca gttcggcaat aagggcagac gactggcatt
     6661 tctttatcat tatgttcgac tacattattg acttcttcgt atgcaaattt ttttccgcaa
     6721 ttagggcatt tcattttttc catgttatct caacttcctt tcatctttac tctcttagct
     6781 tactcttata ttgtaaaaaa gagaagcaat atccttcttc tatgcagata tccatacctg
     6841 acggtcctgt tctccatcta ttaatcggac tttataataa ggacgttcat ctttgactag
     6901 aagtgtccac tctttcactt ttgctttttt cccgtagtag ctgactgctt ccttcatcgc
     6961 tgcttgcggt tgtttgaact cttccgtttt ttctatatca aaaagatgtt ctgcggcctt
     7021 ttccgtgtca tttcgtacgg ttgttttccc agtggatgga ttgactgcta tgacttgtcc
     7081 ttcacaaaaa aactggtatt catatgttgc attcgtagga ttctccaaat ctttttcaac
     7141 tgcaaaatga atagcagata tgttatgttc ttttgtttcg ttttttacga ttgctgctgc
     7201 ttttttcaat gagatccgat attcctggtc actaatatca tccatgtgaa aagcggcatt
     7261 gagcagtccc gtcataccgg taatccctat tacgatgagg gcagcaacac caaacatcat
     7321 ttttttcatt cttcattctc ctgtttcttt tttttaaaga atatcaagaa atgaacgaat
     7381 gaaaaatcga atgaaacgaa aaaaaagtga caattttgtt atctttattc gtgcagttct
     7441 aatggcaatc cgtctggatc aaagaaaaac gtcatttttt ttcctgtaaa ggtatcttct
     7501 ctaatcggtt ctgattcgat ccctaaggac tgcaattctg caattgtttc ttcaatatcg
     7561 tctactttga aagcaagatg acgcaagcca caagcttctg gatagtttgg tcgttgtggc
     7621 gcattagcaa ttccaaaaat ctccaattca gcacttccta gtttcagatc taatttatag
     7681 tcatctcgtt ctttgcggta attttctctg attacttcaa atcctaattt attaacataa
     7741 aaatcacgtg atttctgata atctgaaaca atgatggcta tatgatggat cgttgtaagg
     7801 ttcatatgag cactcctatt cttcgtttaa gctcggatct atattaatct ccagttccat
     7861 tgtaaatgtg tcagattgaa aaaaatagct gtttttcctt tttataagaa aaaaggcaaa
     7921 aaaaacagaa agtaggtcct ccaagacact cttcctgttc ccaatataat aaatgaaagt
     7981 aagaaattat attttcaaga aaacaaacag aaaattatca taacacaaat tatttataat
     8041 tgaaagacta gttttttttc aagaaaagca tacaatagat tgtgttagta aatacagaac
     8101 acactacatt tagtgtctta aggagtcctc atacatgaag tcatattttc agtttttagc
     8161 tcatcagcta aaaggctggc cgcaacaaaa ttattatctt ttctttttca gtcttggctg
     8221 ccaggtcatg actcttgtca atagtcccat cacttggctg tccgttatta catttatcgg
     8281 taccacgctt ggtgtattgt gtattttatc catcaatgcg gcaaaatcag tcaacggtat
     8341 tttaggtatt ttgtctgcga tttgttttat tatcgttggg ttttcagcaa agaactattt
     8401 aagtatcggt gaacagattg cttatatgat cacattggat atccccgtat tgttaagtaa
     8461 agaatggaat cataatatgg cttcaaaaat ccgaaaattc actggaaaaa gctggtttat
     8521 cgcaatcatc agtactttcc ttgtctatct tgtttcagga ttcctgattg gtcgttatac
     8581 agatgatcct cggccgtggg tagatgccat tgctttttct attagcttga ctgctggtat
     8641 catcagtttc ttgcgttaca acaaccaata ttactggtgg ttagcttcag ggcttgctca
     8701 aatggtcctt tggtatattt catttacgca tggatctgca agtttggcga tgttcgtgaa
     8761 cagctctgtt tacttgctga atgatgtcct tgcatttacc gtttcacctt ggtataacca
     8821 aaaagaacgt gaacgtatgc aaaaaatgga agaagaatac tacaacgcac aacaacacgc
     8881 ataaaaaaac tgagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
     8941 tataaacggc ggaatcagaa gcaactcctt cggaaataag ctgaaattca caaaaatttg
     9001 aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
     9061 ct
//
LOCUS       DAZKNH010000102         8942 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000102 DAZKNH010000000
VERSION     DAZKNH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.102"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..260
                     /locus_tag="ACG8MG_002270"
     CDS             <1..260
                     /locus_tag="ACG8MG_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376284.1"
                     /translation="LKRKVTDRNKNLLNVVMICYISFLILGLPQAVFPEMFSGDWGIV
                     SYSILTLIAMVLGTSFYNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="ACG8MG_002271"
                     /pseudo
     CDS             467..667
                     /locus_tag="ACG8MG_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="ACG8MG_002272"
     CDS             complement(1379..1984)
                     /locus_tag="ACG8MG_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0376285.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="ACG8MG_002273"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="ACG8MG_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2666..2881
                     /locus_tag="ACG8MG_002274"
                     /pseudo
     CDS             2666..2881
                     /locus_tag="ACG8MG_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3019..3990
                     /locus_tag="ACG8MG_002275"
     CDS             3019..3990
                     /locus_tag="ACG8MG_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0376286.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="ACG8MG_002276"
     CDS             4130..5443
                     /locus_tag="ACG8MG_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFQ0376287.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="ACG8MG_002277"
     CDS             5486..6352
                     /locus_tag="ACG8MG_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0376288.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="ACG8MG_002278"
     CDS             6349..7197
                     /locus_tag="ACG8MG_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0376289.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="ACG8MG_002279"
     CDS             7211..8683
                     /locus_tag="ACG8MG_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFQ0376290.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..>8942
                     /locus_tag="ACG8MG_002280"
     CDS             8700..>8942
                     /locus_tag="ACG8MG_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0376291.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2830 a         1515 c         1831 g         2766 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aa
//
LOCUS       DAZKNH010000103         8776 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000103 DAZKNH010000000
VERSION     DAZKNH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.103"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(374..2755)
                     /locus_tag="ACG8MG_002281"
     CDS             complement(374..2755)
                     /locus_tag="ACG8MG_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0376292.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2745..3392)
                     /gene="recU"
                     /locus_tag="ACG8MG_002282"
     CDS             complement(2745..3392)
                     /gene="recU"
                     /locus_tag="ACG8MG_002282"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFQ0376293.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3456..3995
                     /locus_tag="ACG8MG_002283"
     CDS             3456..3995
                     /locus_tag="ACG8MG_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFQ0376294.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4085..4504
                     /gene="gpsB"
                     /locus_tag="ACG8MG_002284"
     CDS             4085..4504
                     /gene="gpsB"
                     /locus_tag="ACG8MG_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFQ0376295.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4526..4893
                     /gene="rnpB"
                     /locus_tag="ACG8MG_002285"
     ncRNA           4526..4893
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8MG_002285"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5102..6268
                     /locus_tag="ACG8MG_002286"
     CDS             5102..6268
                     /locus_tag="ACG8MG_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFQ0376296.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6314..7810
                     /locus_tag="ACG8MG_002287"
     CDS             6314..7810
                     /locus_tag="ACG8MG_002287"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFQ0376297.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7891..8574
                     /locus_tag="ACG8MG_002288"
     CDS             7891..8574
                     /locus_tag="ACG8MG_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0376298.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8583..>8776
                     /locus_tag="ACG8MG_002289"
     CDS             8583..>8776
                     /locus_tag="ACG8MG_002289"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376299.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2773 a         1585 c         1851 g         2567 t
ORIGIN      
        1 agacaaatta tgataattaa gcatactatc agataactca aaatgaagcc ttctttcaaa
       61 aaattattca aaaccatata ttgtattttt tatttgataa taacaatggg attattaaga
      121 agtggcacta aataaaagac tagtgtgtat ttagccgatt taattataaa caagtaaatg
      181 gataaagtat atgtaatgat aaaagaaaaa tggattttct aatttttaag ctaaatgtta
      241 gatcacacat gtatatgctg gatagtctta taaaaaatca tgtaatacag cttggtacat
      301 gatttttatt tgtagctgtt ttttacatag acacaaaaaa gaaaccacgc ttgtagacgt
      361 ggtttctttt ttactatctg tttccagaag ctgaggtgct gctggatggt gtatggtcat
      421 ttgcagcatc cgctccgcta gttgctggct cactggaaga agcaggagga gcagatgaag
      481 acggtgtcga gctttcttcg gatgaagaag aaggaaccgt tgttgaagac gctggctcgc
      541 ttgtttctgc tgtcgtgctt tcttttgagg actcagcagt tgattcgctt tgactagtag
      601 agctagagct ttgagtcgtt gtctctgttg ttgagcttcc aggagtctgg acatagctgg
      661 aagatggaat agttgttgat ggtgtaattg cattcgatct agctgtatat tgatctttgt
      721 agtacagttc accgcccaca cgaattaatc cgcttggcat ctcccagtct gtatttgtca
      781 cgttagctga aacatactgc ataagctcgc gataaacatc agaggctacg tgtgacgatt
      841 ctgaagtgac aggcgtcatt tttttattgt aacctgtcca cacggaaatc gaataatttg
      901 gcgtatagcc cgcaaatagg atatctggat acaccccaga agaaatacca agttttgcat
      961 actcgtcatc tgtgtaattg gatgttccgg tcttaccggc ttgatataac ccagcgattt
     1021 gcgcattcgt accagttcct tcagtgatcg tatcttttaa aatgtctgtg atcatataag
     1081 ctgtttctgg agacatggct gtttttccat ctggctcata agtttcttct gttccgtctt
     1141 ggaagacgat tttattaaca tattgaggtt tgtaataagt ccctccattg gcgaaagccg
     1201 cataggctgc tgccattttt aaggaagaag ctccgtattt tgtcccatct tgttcttccg
     1261 tattactaga gatcgcattc gattgatgga tcgtactgta ttcgatccca agatttttta
     1321 agaaagaagc cactttatct gaaccgactt cattgaataa tttgactgct ggaacattac
     1381 gggataagta aagtgcttgg cgtaaagtaa tggtgcccat atattgatta tcccagtttc
     1441 ctacaggagt cgaagtccct tcatagttat acggtgcgtc agtaatagtt tttccagtag
     1501 aatatttcaa atattcaaaa gctggtccgt aatctgtgac aggtttcatc gtagaaccaa
     1561 aatcacggga agtattcact gctaagttgt tgcctaaagt tacgtcttct gcgatgtgac
     1621 gtccgccaat ctgggctttt actttcccgg tattggtgtc gataagggtg gaggcaactt
     1681 gcatctcgtc gtctgggtaa gagacatact gatccgtgtt gacgatatca tatagtttct
     1741 tttgggcgtc cagatccaaa ttcgtataaa tctctaaacc gtctgtgtag acattcttat
     1801 ctgttttttc ttgtacttca ttgatgactt ctttgacata attatcgacg atcttagtat
     1861 tgtcatcgga ttgagttagt tcttgcagac catccgttac cggtacatta acagcttgat
     1921 catattctgt ttgagagatt ttttcgtttt gaagcatcgt atacaaaaca gtatctcgtc
     1981 ttttcttcgc ctgatctgga taaacataag gatcataggc ggaaggggct tgaggcatcc
     2041 cagcaagcaa tgctgtttgc ggtaaagaaa gttctgataa tttcttacca aagtacatct
     2101 cagaagctgt ctccatcccg tacaagccgt ttgacatata cactttattt acgtagtagg
     2161 tcaagatctc ttgtttcgat tttttttgtt ccaatcttac agccatccac gcttcttggg
     2221 ccttgcgttt cagagtctga tcctcggcac tggtggagaa gaaggaaagt ttgatcaatt
     2281 gctgtgtcaa agtacttccg ccttgcagtc cgccagaggt aaagttagag agggcagaac
     2341 cgatgatccg aatagggtca accccgatgt gtttataaaa acggcgatct tctaccgaca
     2401 cgatcgcatc ttctaatgtt ttcggtaatt cattcgcaga aattttttca cgcttttctg
     2461 cgcctaaatc ttcgaatagt tcgccatctt gtgtatatag tttggaagat acggtagcgt
     2521 ctaatttttt atccgtcaat tcgggcgcat cttttgcgta ataccagaaa agtcctacac
     2581 ctgctaaaaa gaggatacag aaaaatgaga gtattcctaa aaggatcttg ataaataatc
     2641 cttttttatg tgttccagag gactttccag aacctttgcc agaattcttc ttcacactct
     2701 ttttaggagt aggctgtttc tgtcgtctag agcttctagt ttgttcattt gccattcttg
     2761 ctttctcctt caatagataa tgttgataaa tagaagtcaa ccgcgtccaa ataaggaatc
     2821 cgcggagaaa taccagtttt gatctctatt cctagttctt cgatcaaaga taaaggtagt
     2881 gatttgcgtc ctttttcctt ttgggcgtcc cagtaagcaa tcaaatgaac actattcaaa
     2941 taaaaacagc gcttcaaaga cgaaaaccat aacaagacaa agcagacccc attttgttcg
     3001 aggcatgctt tcatatgttc gatttgatgg gcatggaagt ttttaaaggg aaaagatgtt
     3061 ttgttcttgg tttcttttgc ttcaaagtcc aagtaaaagc ctcgatagac gccattatag
     3121 tctgtagttg aggcttgggt aaagtaggct tccttgatca cagcagcact tctcttagga
     3181 tagtctactt tcacgatttg gataggggta ggctttttat ggataaccgc caacccgcgg
     3241 gaaagataat atttattact ttcattgatt gcttcttcaa agcgcatccc ccgattgccg
     3301 aattcagtga tctgatttct tttttcttga attttttcga cgggagaggc attcttataa
     3361 taaggttggc catttggata gcgcaatacc atctttaatc acactcctag gccacattat
     3421 agcaaaaaag cggatagaaa gaaaaggaat ctgagatgga taagtcaaaa gtaatctatg
     3481 tgtctgggta tcgaagtttc gaactcggtg tttttaaaga aaatgatcct aaaatccaag
     3541 ttgttaaaaa tgttttaaaa aaagaactcc atcagttagc agaagaaggg ctggaatggg
     3601 tacttgtcag tgggaatttg ggcgtcgaac tatgggctgt gcaaatagta gcggaattga
     3661 aagaagaata tccggaacta tctcttggta ttttgtatcc tttttctgat tttggtaata
     3721 actggaatga agggaaccaa gaaaaaaaac aattagcaga acaattagca gattatgtcg
     3781 aagctgttag ccatcaaccc taccaatcac ctagtcagct aaagaatcac actcgtttta
     3841 tacttgagca tacagtgggc tgtctgctga tctatgatga agaattccct ggaaaaacga
     3901 agtatttttt tgaagatgca cagaattacc aagcagatca tccctacgaa attcggctga
     3961 tcagtatgga cgatcttcaa aattttagtg aataatcgat attgtgtttg atttatcaac
     4021 ttttttccta tacaatgaaa aaggtagaaa tgttattacg taaatgaaaa ctagaggtgt
     4081 aaatatggcg aatttggttt atagtcctaa agacatttta caacaagaat tcaaaacaaa
     4141 aatgcgcggc tatgatcccg ttgaagttga tgaattctta gacaatatca tcaaagatta
     4201 tgaaacttac agcaaagaac tccttgcttt acaagaagaa aacgatcgat tgagtgcaaa
     4261 agtagctcaa ttatctaaaa ctcaaggagc agctcaaaca cgcgtgcaac aaacagaggc
     4321 accaaagagc gcagcagtga ccaattttga tatcttaaaa cgcttgtcaa atcttgaacg
     4381 tgaagtattt ggtaaaaagc ttgatcaaca agcaagtgca gtcaaaccag cacaacctaa
     4441 tccaaataac tacacaaatg cagacacaag cttagatgat aatgaaaaaa cacgtcaatt
     4501 ttaatttttg attaatgaat ttgcggtttt cgggtaatcg cggtctgact tgatcaggct
     4561 gaggaaagtc catgctcgca caggctgtga tgcctgtagt gttcgtgctt agcgaaacca
     4621 taagctaagg tactctttgg agtaacggca ggaaaacaag ctaaggcttc ggctatgctt
     4681 aagtatcctt gaaagtgcca cagtgacgaa gcggctgggg aaacccatcc ggtggaacgc
     4741 ggtaaacccc tcgagcgagc aacccaaaca atggtagggg cgctttttgt ctggaagaag
     4801 aacaggacaa aaaggcagta taactgcaga tagatggtta ccgctttgtt ttttctccct
     4861 ggaaaagcaa agtacagaac atggcttata gaaaaccgca aacatcaggg aattaggtcg
     4921 tgacaaaagt aatgcgttat aagaaacaag aaaaacattt gaaagctatt tttcaatact
     4981 tcagatgaat cgttcttatt acaaaacaaa cttttgtatc accggttatt cggaatttga
     5041 gtaaaagtgt ggggtaggac tgatgtccca cctccttttt ttataaataa ggagattgtg
     5101 tatggaaaaa aacaggaagt tcaacctcgt cgcaactgcg gcaagcgggc tagaagcact
     5161 ggttggtaaa gaattaagag atcttggaat cgaatgccaa gttgagaacg gacgtgcacg
     5221 ttttgaagga acaatggaaa cgatcgctac agctaattta tggttacgta cagcagatcg
     5281 agtaaaaatc gtggttgggg aattcgatgc gtatacgttt gatgaattgt ttgaaaacgt
     5341 aaaagcattg ccttgggaag attttcttcc actagatgca gcttttcctg tagctggaaa
     5401 atcgatcaag tcgaaactat atagcacacc tgattgccaa gcgatcacaa aaaaagcaat
     5461 cgttgaacgt ctccgtacct attatcaccg tccagcttct gtaccactga cggaaactgg
     5521 cgcacatttt cagttggaag tagctttgct gaaagaccat gtgatggtaa ctctggatac
     5581 gacaggacca agtttattca aacgtggtta tcgcttagaa aaaggtggag caccattgaa
     5641 agaaaacatg gcagcagcac ttattatgtt gacaaattgg cgaaaagacc gtccatttta
     5701 tgatcctgta tgtggttcag gtacgctttg tattgaagca gccttaatag gccataatat
     5761 cgcaccagga ttcaatcgtg agtttgcttg cgaatcttgg gattggttca gtcaagaaat
     5821 catggaaaat gttcgatcag cagccgaaga aaaagcagat tatgatattg aattagatat
     5881 tactggttca gatatcaatg gacggatgat tgaaattgcc aaagccaatg cagaagaaat
     5941 cggattaggc tcttcgatta cctttaagca acaagcagtc aaagacttca aaacggataa
     6001 agaatacggg gtcatcgtgg ctaatccgcc ttacggagaa cgtctaggag aagaagaaac
     6061 tgttcggaaa ttatataaag aaatgggcga tgtattccgt ccattaaaaa catggagtaa
     6121 atatatcttg actagcgact tagcgtttga ggaattttac ggacaaaaag caaccaaaaa
     6181 aagaaaattg tacaatggtg cattgcgtac ggatctattc cagtattggg gaactcgtcc
     6241 gcctagaaaa aaagagaaag acgaataatc ttactaattg attcattcaa ttggatgttt
     6301 ggaggaatag ggaatgaaag aaaaagagtt tttacaagaa gtaaaagaaa tcaatcttct
     6361 acaacaagct ttaggaatcc tcgattggga tacacaaaca ggtatgcctg aaaaagctag
     6421 tgcataccga agtgaagtag acagctatct ttacagcatt tatttttcaa aaaaaatcgg
     6481 tccaaaaatc aaagaagcca ttcgctattt ttctgaacat ccggaagaat tgtcggaagt
     6541 tggaactgcc gtttttgcca aagtaaaaga agagtatgat ttggaacatc gcgtaccgga
     6601 agatcttatg agaaaatata cagctgccgt ttcttcagca cacggacaat ggcaaaaagc
     6661 tagagaaaca caacaatttt ctgattttca acacgcattg aatcaaaata ttgaattaac
     6721 aaaacagttg ataccttact ggagaaaaga tgaaaagacg aattatgatg ttttgctgaa
     6781 tcaatacgaa cccgggatga ctgtcgaaat ccttgataag gtatttgatc agcttaaaga
     6841 aggaattctt tcgatccgcc gtacgctcca agaaaaaggg actgaacctg aaacagactt
     6901 tttaaatcgg agaatgacga aaaaacaaca gaagagattt gtagtgaaag taatcgaaca
     6961 acttggttat gattttagtc gtggtcgttt agatgacact gtccacccat ttatgactgg
     7021 aattaatccg aatgatgcac gaatcacgac acgatggaac gaaacagatt tcaaaatggc
     7081 tgtttttgga gtgatccacg aggctggtca tggtatgtat gaacaagaca tcgatgataa
     7141 atatgcgtat acaccggttt atgaaggtac atcaatgggt atccatgaat cacaatcgtt
     7201 gtttaacgag atcattattg gaagtagtcg cagcttctgg aaaaagcaat atcctttctt
     7261 tcaagaatgt gcagaaggaa cattcgatga tattcctttt gacaaatttt atcgttcatt
     7321 gaaccataca aaatcaagtt tggttcgtat cgaggcagac agtttgactt accctctcca
     7381 tatcatcatt cgttatgaaa tcgaaaaaat gattttcaat gaaggggtag atgttgctga
     7441 actacctgaa atatggaatc aaaaatatga agagtatctg ggaatacgac caacaaatga
     7501 tgtggaaggc atcctacaag acgttcattg gtcaggtgca agctttggtt attttccctc
     7561 ttatgcatta gggtacatgt atgcggcaca attacttcat tctttgcagc aagaaataga
     7621 tgtggaaaat gttctagcgt cagacgatta ttctccaatc aaaaactgga tgtcagagca
     7681 tatccatcaa tatggtgcat ctagaaaacc aaatcaatta atcatggatg caacacatga
     7741 accgcttaat ccgcaatacc tgatcgacta tatgaaagac ctgtatttta atgtatatca
     7801 ggtaaaataa aatacaattt acaaagatag ctgaaattca tctaatctat agatgttttc
     7861 agctatttac ttaagaaaga agtggaacga atggataaat tacaagtgat cgctgattat
     7921 agtatggacc aattagcgca tgatcaaaca ggacatggga gcgatcatac caaaagagtt
     7981 gtcaagttag ctgaaaaaat tttggagacg gaaccgcagg cagaccgctt tgtgacgctt
     8041 gcagcagctt acctacatga tacgattgat gataaagtcg ttaaaaatga gaacgaggca
     8101 aagcaacagc ttcgtgtatt ccttcgctct ttgccaatag tagaggaaca gatctcgatg
     8161 attttttcca ttatcgaaaa tatgtccttt tctaaaaatc tctctgaagc ggccgaacta
     8221 tctctggaag gtaagatcgt tcaagacgct gatcgtatcg aagcattagg agcaatcggc
     8281 attttgcgta ctgcttattt cggcggtgga catgggcacc cgatttttga tcctgaactg
     8341 tatccgcaaa cattcaagga caaagaaaat taccgaaaag gcacgacagt gatcaatcat
     8401 ttttatgaaa aactgttctt gctgtctgat aaaatgaata ctgattacgg atatgaagaa
     8461 gccaaacgaa gagaaacctt catgcgtgaa ttcttagaag aattttttct ggaatggtct
     8521 attgacgatg aaaattttac tccagacaat tggatcaaaa ataacaaaaa atgatggagg
     8581 ttgtgacaga agtggtcagc tccaagatat ttcggatcat tttccgtaaa agttacttct
     8641 ggaacactgt ttaccaagag gtcgtgagcc tgccgttcag ctccgagtca caaggaacaa
     8701 tttcaaaaaa tagcttctcg tgtttgatga atattgtaac ttgtgattcg gaattggcag
     8761 gagaacaccg tttatt
//
LOCUS       DAZKNH010000104         8574 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000104 DAZKNH010000000
VERSION     DAZKNH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.104"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..828)
                     /locus_tag="ACG8MG_002290"
     CDS             complement(<1..828)
                     /locus_tag="ACG8MG_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0376300.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     "
     gene            974..1777
                     /locus_tag="ACG8MG_002291"
     CDS             974..1777
                     /locus_tag="ACG8MG_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFQ0376301.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1955..2902
                     /locus_tag="ACG8MG_002292"
     CDS             1955..2902
                     /locus_tag="ACG8MG_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0376302.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2970..3263)
                     /locus_tag="ACG8MG_002293"
     CDS             complement(2970..3263)
                     /locus_tag="ACG8MG_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFQ0376303.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(3291..3956)
                     /locus_tag="ACG8MG_002294"
     CDS             complement(3291..3956)
                     /locus_tag="ACG8MG_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFQ0376304.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(4096..4728)
                     /locus_tag="ACG8MG_002295"
     CDS             complement(4096..4728)
                     /locus_tag="ACG8MG_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0376305.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(4735..5802)
                     /gene="liaS"
                     /locus_tag="ACG8MG_002296"
     CDS             complement(4735..5802)
                     /gene="liaS"
                     /locus_tag="ACG8MG_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFQ0376306.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(5799..6530)
                     /gene="liaF"
                     /locus_tag="ACG8MG_002297"
     CDS             complement(5799..6530)
                     /gene="liaF"
                     /locus_tag="ACG8MG_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFQ0376307.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(6699..7178)
                     /gene="greA"
                     /locus_tag="ACG8MG_002298"
     CDS             complement(6699..7178)
                     /gene="greA"
                     /locus_tag="ACG8MG_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFQ0376308.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(7314..8489)
                     /gene="mltG"
                     /locus_tag="ACG8MG_002299"
     CDS             complement(7314..8489)
                     /gene="mltG"
                     /locus_tag="ACG8MG_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFQ0376309.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         2547 a         1761 c         1330 g         2936 t
ORIGIN      
        1 atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct cgcttagtag
       61 ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat actgccaaat
      121 atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt ccattttttc
      181 atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga tcaccattat
      241 cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga tccagtagat
      301 atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca gtgtatcact
      361 taacaaatgc aaataactag attttatatt caaactttca tctgcttctt gtcgtaataa
      421 taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca tcaaatgact
      481 atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa acaaggataa
      541 tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct ggtagccata
      601 cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag aaaaaataat
      661 tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac tccctgacat
      721 aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta ctccaaatag
      781 tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt tatttatatt
      841 ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt cagctgtttc
      901 cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg acactactag
      961 atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac gattcaaaga
     1021 cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt tagttttgag
     1081 tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca acattcaatt
     1141 ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg ggcagcttca
     1201 aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca catttgatcc
     1261 agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt ttgttagtgt
     1321 cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga cttctgcttt
     1381 gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa acttaacagg
     1441 taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa aagtcctgac
     1501 atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc tatttaccaa
     1561 cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc ttgattgttt
     1621 aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct tgatggtctt
     1681 tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta tttttgctca
     1741 agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa ctcgttattt
     1801 ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc cttttttatt
     1861 tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc ttgtatgcgc
     1921 tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca tttatgcaga
     1981 tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg gttatatctt
     2041 taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg gctggaactc
     2101 agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat ctatctcagc
     2161 tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac tttcaaatgg
     2221 tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc ttgggtattc
     2281 tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag gttcatcggt
     2341 ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc atattggaaa
     2401 aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca atacatccgg
     2461 tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg aagttcttcc
     2521 tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg ggctctttga
     2581 tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg gccgtggtgc
     2641 ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt cctttgcagc
     2701 attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga aaatggacaa
     2761 tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga aattaatggc
     2821 tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa ccaatcaagt
     2881 aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa aaagagaagg
     2941 tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata ggttggctcc
     3001 tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca ttcatctgct
     3061 tcctcgataa aaaaaagtgt cccatcaatt tccactttct ttacaggatg ttctggctgt
     3121 tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta gacttttcca
     3181 aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca cttttgtgct
     3241 tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt ctattgattc
     3301 atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag attatcattt
     3361 tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac tcttcgtatg
     3421 gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt tttgttgaaa
     3481 aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa ctctaggtaa
     3541 tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc tcgctcggga
     3601 tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc attttgggct
     3661 tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa gatactggct
     3721 tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc taatgatcga
     3781 agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt catatattca
     3841 ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc gatcagtgtt
     3901 cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg tttcaccgtt
     3961 ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa caaaagcaag
     4021 catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt ttgattatcc
     4081 tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag cggcttgtgt
     4141 acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta ctgttttcag
     4201 tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa tcagcattaa
     4261 aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc gccgactcat
     4321 tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac gttgcgttgc
     4381 ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc ctgctgcacc
     4441 tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga taatgatttt
     4501 agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca tttcttccat
     4561 gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat agccttgtcg
     4621 tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat aaaaagagac
     4681 gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc ttatctatgc
     4741 ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa aactaatgat
     4801 ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc cataacttcc
     4861 cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta acaaaagatt
     4921 attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc ttaatgtatt
     4981 tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag atagttttac
     5041 atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct tcaataactg
     5101 ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat gaagaagcag
     5161 cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca actgcttgcg
     5221 aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca tcatcatcat
     5281 tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc gatgtctttc
     5341 taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc tcgttacaat
     5401 ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt ccttatcaat
     5461 atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca atttggattc
     5521 ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt ttgtcttttg
     5581 aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg ctaacaaatg
     5641 gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc aatgactttg
     5701 attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag ccgcaatccc
     5761 cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat cacctcgaca
     5821 tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc ataatcactg
     5881 ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa caagacattt
     5941 cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa gatccgtgtt
     6001 ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag cgtattgccc
     6061 aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca ttcatagacg
     6121 tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt attatgtgtt
     6181 tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc atttttggtt
     6241 aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc aaatacagcc
     6301 atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact tcctaatatc
     6361 aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata tatattgaaa
     6421 ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca aacagcaaat
     6481 aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat cgctctttct
     6541 ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc tataggactt
     6601 tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat ctagagtgta
     6661 tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc tactttgacg
     6721 attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt tttacgtttg
     6781 ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa cggatctgct
     6841 tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc gatgaatgta
     6901 actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat gatttgagca
     6961 aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg ttcatctttc
     7021 gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat tttgattcgt
     7081 tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc taatttttct
     7141 ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc cttttttcga
     7201 taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt accacgcttt
     7261 tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt tttctaagaa
     7321 ttatttacat atttttccac taattccata tgttcttcat aagttttagc gaaataaaca
     7381 tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg ttctaatact
     7441 gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc ggtattggta
     7501 tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc tttgtgttca
     7561 cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt gattcggtta
     7621 aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt ttcaactaga
     7681 gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc tgaataatag
     7741 ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt aagcgaagta
     7801 ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta ttttgtgttc
     7861 tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc gttaaagaaa
     7921 gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac cttcgctacc
     7981 cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc atctgcaact
     8041 tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc aagagtcata
     8101 ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa ttttgtataa
     8161 taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc accaatctgc
     8221 ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga tttatctaaa
     8281 ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc gccaataatc
     8341 aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc ttttttacgc
     8401 agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc ttttttatct
     8461 ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca agttcgtaac
     8521 tttattcatt atacatgata tttgaattgg cgtgaatgat tttatttaaa tatt
//
LOCUS       DAZKNH010000105         8452 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000105 DAZKNH010000000
VERSION     DAZKNH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.105"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            528..797
                     /gene="rpsN"
                     /locus_tag="ACG8MG_002300"
     CDS             528..797
                     /gene="rpsN"
                     /locus_tag="ACG8MG_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0376310.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            812..991
                     /gene="rpmF"
                     /locus_tag="ACG8MG_002301"
     CDS             812..991
                     /gene="rpmF"
                     /locus_tag="ACG8MG_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0376311.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            1023..1400
                     /locus_tag="ACG8MG_002302"
     CDS             1023..1400
                     /locus_tag="ACG8MG_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376312.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1421..1570
                     /gene="rpmG"
                     /locus_tag="ACG8MG_002303"
     CDS             1421..1570
                     /gene="rpmG"
                     /locus_tag="ACG8MG_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0376313.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1594..2577
                     /locus_tag="ACG8MG_002304"
     CDS             1594..2577
                     /locus_tag="ACG8MG_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0376314.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2607..2729
                     /locus_tag="ACG8MG_002305"
     CDS             2607..2729
                     /locus_tag="ACG8MG_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0376315.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2747..4270
                     /locus_tag="ACG8MG_002306"
     CDS             2747..4270
                     /locus_tag="ACG8MG_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0376316.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4294..4533
                     /locus_tag="ACG8MG_002307"
     CDS             4294..4533
                     /locus_tag="ACG8MG_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0376317.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4726..5187)
                     /locus_tag="ACG8MG_002308"
     CDS             complement(4726..5187)
                     /locus_tag="ACG8MG_002308"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0376318.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5382..5600)
                     /locus_tag="ACG8MG_002309"
     CDS             complement(5382..5600)
                     /locus_tag="ACG8MG_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0376319.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5718..7334
                     /locus_tag="ACG8MG_002310"
     CDS             5718..7334
                     /locus_tag="ACG8MG_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0376320.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7575..>8452)
                     /locus_tag="ACG8MG_002311"
     CDS             complement(7575..>8452)
                     /locus_tag="ACG8MG_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0376321.1"
                     /translation="SLSQLLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPIKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKGDRRSKHLKKYWKLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSVIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYQT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGYRNFQNYRERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
BASE COUNT         2905 a         1293 c         1677 g         2577 t
ORIGIN      
        1 gatgatgaaa taggctttaa attaacctac aatttttggg atatagttat aattcttttt
       61 caaaaagaat aattttatta aacagcatct aattttgaat acacgaaaga atcagtatga
      121 ttgaattagt tcacttaatg tctattctta taatataagg aataatcaaa gttttaaaaa
      181 attggcgata aaccagttaa acataaaaga ttgaataaaa agttatagtt tctttcatat
      241 tgccgtttta agtagaggaa aagctactta aaacggtatt tgtgtttgat tggactaata
      301 aaagtgaatt cttatttttc agagcctaaa ctgcatattc ttttaaatga tttgattatc
      361 tctatgtacc gataaattcg ttttcaaata gttatctatt catatgagtt ttatttcatg
      421 ttatttattg ttgacaagag caactcttgt tggtaaagta tcagtgtcaa aacgtaacga
      481 ttacgatttg aggtgtctaa atcaaaataa aaggagtata aaggtatatg gcaaaaaagt
      541 cgaaaattgc taaagcaaag aaacaaatgg cgatgattga aaagtatgca gataaacgtc
      601 aggaattaaa agccgcagga gatcgcaccg ctctcgctaa attgccaaga gattcaaatc
      661 cgaatcgtct gaggcttcgt gatcagacgg atggtcgtcc aagagggtat atgcgaaagt
      721 tcggtatgtc ccgtattaag tttagagaac ttgcccatca agggttgatt cctggagtaa
      781 aaaaagcaag ttggtaaaag gaggaaatat catggcagta ccagcaagaa aaacatctaa
      841 ggcgaagaaa cggttaagaa gaactcatca aactgtagta aaaccagaaa tttcatttga
      901 cgaagcgaat ggtgattata gacgtagcca tcatgtatct ttaaaagggt actataatgg
      961 aaagaaagta attaaaggag cttcaaaata gtttaatata taacttttag gagggaatca
     1021 aaatggaaat tatttaccca cctttagttg aagaaggatt aaagtattat cttgaaacga
     1081 cccagcaatc gctagataaa tgcacttttt atcgttcgat ggtagagaga ggaattatta
     1141 ctgaaacggg tttaccgacc caacaggcaa ttgaaaatgg attagtcaaa gattactatg
     1201 aggaccaagg cttatcattt gatgattttt taagaatcta tccaattttt gaagagtatg
     1261 acgaggagtt atttcaatgt atcgatggtt attgggagat acctgttgat atgaaagaaa
     1321 atttagtttc acaattggaa tctggcgagt taacttttga agatgcacaa caaattcaag
     1381 catatcttga agatcgataa gtattgaagg aggaagattt atgcgtcaaa atattatttt
     1441 ggaatgtgtc gaaactggag aacgtctgta tctcaccagc aaaaataaac ggaataaccc
     1501 tgaacggtta gaattaaaaa agtattcgcc aaaattgcgc agacgggcaa tttttaaaga
     1561 agtgaaatag ctaagtggta aaaaggtgac aaaatgggca taccagttac aatcgtttct
     1621 gggtttttag gggctggaaa aacaacgcta atcaatcaag ccttgcaagt ctcaccctac
     1681 cccagggaag aaattattat aattgaaaat gagtttggtg aagttgggat tgatcacaaa
     1741 ttattagttc atagcgagga acgggtgtta caactaaata atggatgtat gtgttgcagt
     1801 cttcgcagtg atttattagc aatattgatt tctttactgg aaacaataga agaacatcaa
     1861 caaccaattt cacaaatcat cattgaaact acaggtatag cagatcccca accaatcatt
     1921 caaacacttt taacagcgcc tcaactaaga ggaagagtat atatcgatag tttattgact
     1981 gttattgata gtgacagttt tgaacttatt gaaagagaac cacaagccct taagcaactt
     2041 gtcatggcag atcgcatttt tgtgtctaga aaacaaaact tgaatttatc tgcactaaaa
     2101 aaattacaga gagaaatcaa aagtattaac cctttatccg atattcgaat gttcacacat
     2161 agtgaaacga ttcaaaaaaa ggatttttat aatttgaaaa aatttaatca tcccttagtt
     2221 tttgaaagtg attcatcaga aaatcactat aatggcaaat actatcatca acaccatcaa
     2281 gtacatgagt ttaaatcaat tctcttaact tcagatcaag acataaaaaa agatttttta
     2341 ctccaatgga ttgactggct gattttaacc aaccaaggga gcctgtatcg ttttaaagga
     2401 ttgattgctt tacaagagca ggattttata gttgccatgc aagaggtaaa cgaacaggtt
     2461 aattttcaat acaccactcg acaaaaagac caaaccacta ttattctaat cggaaaaatg
     2521 ttggatgaag aaaggatcaa acagagtttt gataaattac tggcggaaag caaataatga
     2581 taagtagtga ttgggggtaa tttctaatgg agaaaacgga tatatctagt gcatatcgac
     2641 gacttaaaag cccgaatatt aaaactagaa aaagggcatt aaaaattatc aaagaacata
     2701 aacgaaataa aatgaagaaa ttagcataat taaggaagga aaaagaatga agaaaatcag
     2761 tgtaggactt attgggatag cagctctagg attattggga gcttgctcca gcactaatga
     2821 tgcaaaagta tcgaatgata aagatggaaa attagaaatt gtaacaacat tctatccaat
     2881 gtatgatttc accaaaaaca tcgtcggcga tgaagcgaac gtggatttaa tggttccagc
     2941 gggatcagaa cctcatgact atgagccctc agctaaggat atggcaaaag ctcatgatgc
     3001 tgatgttttt gtatatcata acgaaaacat ggaatcatgg gtaccaaaag ctaaagaaag
     3061 ttggaagaaa gctgggccta atgtagtaga aggaacaaaa gacatgattt tattgccagg
     3121 tagtgaagaa gaagatcatg atcacggaga agaagatcac catcatgaac tagatcctca
     3181 tacgtgggtt tcacccaaaa tggcaattaa ggaagtttca aatatcaagg atcagttagt
     3241 taaattgtat ccgaaaaaag caaaagtatt tgagacaaat gctgaaaagt atttgactaa
     3301 actgaaacgg ttggatgctg actataccac tagtcttaaa gaagcgaaac aaaaaagttt
     3361 tgtgacgcaa cacgcagcct ttggatattt agctttggat tatggattga aacaagttcc
     3421 tattgctgga ctaagtcctg aagaagagcc atcttctggt cgattagctg aattaaaaga
     3481 gtatgttaaa aaaaataaga tcaattatat ttactttgaa aagaatgcta atgataaaat
     3541 tgcaaaaact ttggccaacg aagctgggat aaaattggaa gtactgaatc ctcttgaaag
     3601 tcttacaaaa gaacaaatgg ataatggtga agattatgtt tcagtaatgg aagacaatct
     3661 aaaagcgtta gaaaaaacaa ctatggttgc tggtgaagag gtagttccag aaaaagaagc
     3721 gaaggatgaa aagacagttg ctaatgggta ctttaaagat gccgatgtga aggatcgcga
     3781 attgagtgat tatacaggcg aatggcaatc tgtttatccg ttgttaaaag atggaatttt
     3841 ggatgaagtt ttcgattaca aagcaaaaat caataaagac atgacagctg ccgagtacaa
     3901 agattattat actactggct ataagacaga tattgatact atcaacatta aagacaacac
     3961 cattgatttt gtagtcaacg gtgaacatca tcaatacaca tacaaatatg ttggatacaa
     4021 aattttgaat tatgaaaaag gaaaccgtgg tgttcgtttt aattttgaaa ctgatgatgc
     4081 aggtgctggt cgttttaaat atattcaatt tagtgatcat ggaattgcac caagtaaggc
     4141 agagcatttc catattttct ttggtggaga aagtcaagaa aaactctata atgaattgca
     4201 taattggcct actttctatc cagcttcatt gtctaaacat gaaattgctc aagaaatgat
     4261 ggctcattaa aattcaattt ttggaggaaa tacatggtaa gaaaagtatt attgtctatg
     4321 gattccaaga atattgatgc ttatcggcgt tatcgatttg caaagcaaat gagattaaat
     4381 gggagtacat tagctgatat tgctgcaact ttacatgtta gtattgacac agttcataga
     4441 gtattaaaac gggatcctga ttgttgtttt atcaaggtca atactggaag aagtagacgt
     4501 ttagcaaaac ggcataatag cttggccaat taattgataa taccagagta gatcattttt
     4561 cccttatttg gatttgatct gctttttatt atgtcttgaa ttgtcaaatc aagcgcaaca
     4621 ctttatcaaa gaagacttca taaggtgttc tccaatctaa acactttcta ggtctgttat
     4681 tcagtttttc aatcatctga tttatcgttg agttatctac cgtagctatc tctttcgatt
     4741 ttggcaagta ttctcgaatg agtctattcg tattttaatt tgttcattgt tgccaagggc
     4801 atgaggatct ggaaaataaa agggcatatt attcaatgct tctgtaactg cgaaatgatt
     4861 ggagaattct tttcctctat ctggagtgat cgttttgacc gcctcatttg gaatactgct
     4921 taaaagttca atcattttct cgctcacata agcggctttc tttctgggga ccttctttcc
     4981 tgctaaaagg aagcatgatt ttctgtctgt caaagtcact aaacaagtac caccagtttt
     5041 gccagctaca atatcggctt tccaatgacc aaaatcactt ctatcatctg cggattttgg
     5101 acggccatgg atcgaatgag taaggcaaat ctttccacga gactcgacat gattttttgt
     5161 atgtcttgtt tttcctcgat ggtgcaatga acggatcagg cctttattgc cgtgtgagag
     5221 gggttctgta tcgaaaagat cattgcaaat tgcacgttag atcgtgataa aactaattga
     5281 aaagtccata tgttcatgct tcaaacgatt cgagatttcc tctggcgacc actgaaaatc
     5341 gacaaataaa tgacgaacaa ttgaccaaac tcacggatca tttagaatgt gataacgtcc
     5401 gcattttttt cttcgataag cataattttt ttccgctttg gaaggtgaat agtttttttc
     5461 ttgtttcttc tcagttcatg taaaatagta aacgggctat gctgaatgtc gttgccaatt
     5521 atactaatag aaaatccttc ttcgtgaagc agaaagattc tttctcgttc gagtatagta
     5581 agatgacgat actggctcat aattattttc cttctaggag tgttctcgac aaacttatac
     5641 tagttggata tcgattatga ctcattttta tgtgttgcac ttaaagtgta aattcaaggt
     5701 atagaaagag gttccctatg aaaaaaatac ctattacaat cttaactggc tatcttggtt
     5761 caggaaaaac gatacttcta aatcatattc tgtacggtga tcacggaaag gaaattgctg
     5821 tcattgtaaa tgacataggg gaagtaaatg ttgatggggt attagtcgac aagaaaggtt
     5881 ttaaaagaac tggagaaaaa cttgtttcga tgccgaatgg atgcatttgt tgtacattac
     5941 gcgaagattt gattgttgaa ctagatcaat tagtgcaatt ttctgacttg aatcaaattg
     6001 ttattgaagc ttcaggaatc agcaaaccaa ttccaattat tcaagcaatt atattagcta
     6061 aaaacccttt agacattgat ttgacccaac gattagagat tggtgccatt attatagtag
     6121 tggatgtaaa acgtttttgg gataatttcg aaacgggtga aaagattcag gaaagaaaag
     6181 ctgaaggtaa tgtagttgaa gaagaacagg atattcgtgg cttgttaatt gatcaattcg
     6241 agtgttgtaa catattactt ttaaataata gtgacttagt gtcaaccaag acgatttaca
     6301 aaattaacgg tctaattgct aagttacagt ccaatgccaa aataattagg acaattaaag
     6361 gtgaagtaga cttatcgaaa attctaaata caaatctttt taatatagat gagacttcct
     6421 attcagtggg ctggttcaaa gagttagagc ttggctatca aaatcatatt cctgaaacag
     6481 aagaatatgg tatctcaagt tttatctatc gaaatcggtt actccttgac gcacaaaaat
     6541 ttgcccatct tacggataaa aattttcctg gcaatattac aagagcaaaa gggtttcttt
     6601 ggtttgccca acatagtgat ttttcgacga tctttgaaac atcgggtagc tataaaagta
     6661 ttgaatcatt aaattattgg gtagctagtt tacctaaaat tgagtgtcaa atgattatac
     6721 aaaaaaatcc cgaattgaag gaagcctggc atccgttgtt tggtgaacgc attaataaac
     6781 tagtttttat tggtatgaag atggatcaac aatgtattaa ggaacaactt gagggttgtt
     6841 taactgattc tcttaatgca gataatttcc gaaaaatttc accatttcca tggaaaaagt
     6901 ctaatatgtt tagccagata tcgttaatga aatttttaag aaaagctggt gtcaagatag
     6961 gcgatgtcat aattattggc attttgatta ttacctcttt tattccctta tttgtattct
     7021 ctatagaaaa ggagaatgaa acaactgaag atgctatcaa atatgccatc gttcgaattg
     7081 atggcaagga aatggatcgc ttcgatttgg ataagatcaa tcataaactc gtgaattatc
     7141 atcctgcgaa gggacaatat aatattgttg agattaaaga tggaagaatt cgagttaaag
     7201 aagataatag tcctgatcaa atcgcggtta gaactggatg gatctcaaaa cccggtcaaa
     7261 cgagtatttg tttaccccat aagttagtta ttagtattga gaaaaaagaa agcacagatt
     7321 actacattta ctaatctgga aatagatgag gaggttttta ataagaaaaa gatattatta
     7381 ttaataatag caggggcttt attaagtgtg ttaagcgctt gtggacaaga aaaagaagcg
     7441 gtgacaacag gcacaagtat ctttaacggg acctgcaatg gtttagttaa cagtcggtta
     7501 gattacttat tttggctctt cgtcaatgag tgttggtgaa gaaattcaat agaataatcg
     7561 agatactcac acggctagac agctatcttg tctagccgtg ttgttgttct ggctttagtc
     7621 agttgggtag tattcttagt tttcttaaaa tattttcctc gatataaaaa gattcgttct
     7681 cggtagtttt gaaagtttct atagccatag gcaattcgtt ttagtacttt gatatggtta
     7741 ttcaaacatt ctatagggcc gttggaatag ggaacgcgca acgctcgttt gatttctgtt
     7801 tggtatttct tgaaggtcgt gaacactggt tggtagtgct ctggcaactc cttgtagtct
     7861 tcttttagga gcgcgacgaa ttcaggaaca tcttgtcttc tgataacgga aagaaaatcc
     7921 tggtagactt cgtagcctcg ctttaaggaa tcatcataag ataatagccg atcgactatc
     7981 tctgcttctg tcagatgatc tcgaaatgac ggacgccagt agcgatgctg acctttcaat
     8041 tcccacgcat tcttttgaag tagtttccag tatttcttta agtgcttcga ccgacgatcc
     8101 ccttttttaa aggtcttcat tacttgaatt cggtggttgg taaaagcccg accaatgtgt
     8161 tggctaatat gaaagcgatc ggcgacgatt cgggcattcg gaaaacactc tttcaccaat
     8221 gtgcgatagg gtgtatagaa atctgaaacc actagacgca cttgttcacg gacttttata
     8281 gggtagcgca agaaatagtc gcgtaaagga ttgagttgcc gattttccac aatatcaatc
     8341 agttgatggg tcttgccgtc catcatgaca aagctcatgg ctcccgagac atttttcact
     8401 gatttgaatt catcgaaaca caccacttct ggtaacagct ggctcaaaga aa
//
LOCUS       DAZKNH010000106         8448 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000106 DAZKNH010000000
VERSION     DAZKNH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.106"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1324)
                     /locus_tag="ACG8MG_002312"
     CDS             complement(44..1324)
                     /locus_tag="ACG8MG_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0376322.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(1465..2067)
                     /locus_tag="ACG8MG_002313"
     CDS             complement(1465..2067)
                     /locus_tag="ACG8MG_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0376323.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            2312..2518
                     /locus_tag="ACG8MG_002314"
     CDS             2312..2518
                     /locus_tag="ACG8MG_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376324.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            2830..3549
                     /locus_tag="ACG8MG_002315"
     CDS             2830..3549
                     /locus_tag="ACG8MG_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0376325.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(3630..4313)
                     /locus_tag="ACG8MG_002316"
     CDS             complement(3630..4313)
                     /locus_tag="ACG8MG_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFQ0376326.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            4531..6717
                     /locus_tag="ACG8MG_002317"
     CDS             4531..6717
                     /locus_tag="ACG8MG_002317"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFQ0376327.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            7063..7473
                     /locus_tag="ACG8MG_002318"
     CDS             7063..7473
                     /locus_tag="ACG8MG_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0376328.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            7487..7843
                     /locus_tag="ACG8MG_002319"
     CDS             7487..7843
                     /locus_tag="ACG8MG_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0376329.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         3104 a         1384 c         1577 g         2383 t
ORIGIN      
        1 gatcgtcttc acaaatataa atgggaatca tatcattact cctttatttt atttgtatat
       61 aaatcgtttg agaaaaacgc tggttttcaa ttgatgtttc aaggaaaaga taaggattgg
      121 tctttgtcaa ttcatccaag ataaacaagc caaggccttc gttttttgga tttgtttttg
      181 ttgagaaccc tttttgtttc aactggttta acggtaatgt atttgttgtt gtattgatca
      241 cacgaatcat cacggaatga ttcttcttga gtatggtcaa ttcgattttt ttcttctttg
      301 cagcactagc tccttcaatc gcattgtcca agataatacc aagcatacgt acaaggttta
      361 ccgtatgttt cgtatccagt tcgattggct caggaaaatg aagctggacg atcaaccctt
      421 tttcctgtgc catcatgagt tttaatgaca gtaaactacg gatctcagga actttcatat
      481 tatccaacat acttagattg gccggaaaaa ggttcatcat ctggcgtgta ggtaatatcg
      541 tttgatcata ataagaccgt aatccttcta agtcgccttc tgaaatatag ctgtgaagag
      601 aaaaaagcag attttggtaa tcatgtttga attctctcaa gtcttgatag tttttttcta
      661 gatctttgat ataatcctgt tgcatcctga cttgttgctc taataactga tcagctaatt
      721 tcttattaga gattttccaa taataaacca agatgatcag gccaacacac agatatagaa
      781 tgaaaaaact cgtattcaaa gctaagacat caggtgtttc accagaaaaa cgggtataaa
      841 aaataatgat atagtatgta aataatatca atgtcccgat cgtaccgatc atacggcggt
      901 ccacagtgtg aaaccaagtc tctaacgatt tttttaaaca aaaagctaat aaagcagata
      961 aaaaagcgga taagacaatg tgaaaatact gcaatgaatc tcctggttca ataaccgaag
     1021 atttcaaaac aaaaaaatcg ccgatgcttg ttaagtgatc tcctaaaagc gacgtcaata
     1081 acccaaaagg aagcgcgtaa accaattgtt gacgcgaagt cgaaaagaag aaaaaagtaa
     1141 gcatcagcat tcctatagca attagagcag taaaaatccc taaaatcgaa tagacccatc
     1201 caatcaaaat acaagaaatc agaatgactc ccgcaaacag atatttgtat cggatgtgat
     1261 ggtcaatcaa taagatacaa attgtgtaaa tcaaatattg caacgctatg gaccataaaa
     1321 acataaagta cagctcccat tgatttttac caaattatag cacaattggc gtattatttt
     1381 tttaattttt cagggatttt gaattcagaa ctcataccaa agcagcagcc agatacaaaa
     1441 aagtcagata agttagttaa ttgtttaatg attgtttttt tcatgaaaga ttcctccaaa
     1501 attcatcaag tagtttgtaa taggaagcaa cgtagctatt tctaaaacaa tcccaagtag
     1561 caaataggta gaaaagtaag aagctgagaa aaataacgta atgagagaaa aaattagaaa
     1621 catgctgatc gccttcttta ataatggggg acgtttctcg ggttcaatgg gattgattgc
     1681 tgtacctctt gatccatagc gaagcagtaa caaaaagcag ctgattaaca aaaagaccat
     1741 cattgggaag gagatagtca attgcagatg tttcatcacc aagggaacgc cccataaata
     1801 aagtagtgtc cacagcagac aaacgaattc agagtcggaa tgcaatccat aagcatatct
     1861 gcggatgcag atataagaaa gaaaggtgat tcctgtttcc gcccatgagc ctgtcaccag
     1921 agaaaagact agaacgaccg ctagttttcc aagattattc aataaaactt ctaagacaaa
     1981 ttttacttgg acatacacca tatcttcttc cgatttgtct ccgctgaata aggcttctaa
     2041 taatttgttc acaactttat ctttcatctc atacgtacct ccaccacaaa ttttatgcta
     2101 aaaaaaataa aaaacgtctt ttgtttcata aaaagtggtt ttgacatcaa aaccgtcatg
     2161 atctcctaaa aaaatcttcg ttttccaaga accttaccaa aaaaagtgac agttttgata
     2221 cgaaaagtac tttttatgac aagtttgtgc tttttcgagt gatttatgtt aaaaaattac
     2281 tgaccttata aaaggtgatg gagggattct tatggaaaat gaacaattgt ttgaagtcgg
     2341 cgaaacatat gcatttaaat ctgatttatt tgaatcacca ataaaaggaa aaatagaaaa
     2401 aatctatgaa aatagtgtga tgatacgtgt catgaaatgc gcagtagaag acaatgatac
     2461 cgctcataat ctgcactggc agttagttgt acgcaaatca agtacaatag aagaataaaa
     2521 acagataaaa tgttgagagc ataaagaggt tgtgagtctc ccgtttagct ctgagttaca
     2581 aggaataatg ttaaaaaaca gcttttcatg ttttatgaat attgccttgt gaccgaggtg
     2641 ttggcaggag aatactgttt atttgaaaaa agggctgcga caagactttt gtcacagcct
     2701 ttattttcat gaataggagt tacttttggc tacttttaga gaagtagctt ttaaggagca
     2761 gataaagtca aggcttttat ttctataaat gaagtagtaa aatgaagaac agattttatt
     2821 acaggaagga tgccgataat tgtgatcatt gttgcaggaa cgatcggtgc agggaaaagc
     2881 acactaactg aaatgcttgc ccaagattta gaaacaaaac ctttttatga gaacgtggaa
     2941 gataatgaag tattgccgct attttattct aatccagaaa agtatacttt tttattacag
     3001 attttctttt tgaacaaacg ctttttggca atcaaagatg ctttcagcca tgatgacaac
     3061 gtactagacc gctccatcta cgaagatagt atgctgtttc atttgaatgc agatttaggg
     3121 cgtgtttctg aagtagaagt aaagcagtat gaaggtcttt tagaaacgat gctgaaggag
     3181 ttagaagaaa tttcgccaca aaagaaacca gatctactgg tttatattcg tgtatccttt
     3241 gaaactatgt tagcgagaat caaaaaaaga ggccgggaat atgaacagct agaacaagat
     3301 cctgaactct attcctacta taaagaactc aatcgacgat acgaagagtg gtatgaacag
     3361 tttgatatat gtcccaaaat agtgatcgat ggggacaaat atgatttcgt agccgatccg
     3421 gcatgtggag aacaaatcgt acaagaaatc aagatgcggg caaagaaaat gacagaagag
     3481 gcagatgccc agtcgatccg ctacactaca aataacccga aagcaaaggg aatctttaca
     3541 actaaataat ataaaaacta agcaaattgc tcactcgcag atatttctct gtgagcagca
     3601 tcttgcttag tttttttgat tcattgagat caaatagtga accgattcgt atgataaaat
     3661 tgccataaat gaaatgtctg agtgagggcc atgatcaaaa ataaaatgga aacgatcaaa
     3721 atcgaatggg cgccaaacag atgatgacag aaaacaagga caatagctcc tgcgataaag
     3781 gcaagaacga cacttagata gtggaaaaat cgatcaaaat ctgctttttc tttagaaata
     3841 caataatcat ataagttcgt tgctacccct tttaaatttc cagttgtaaa taaattagtg
     3901 taggcacgcc cgttgatttt atcaaaagct acccactgga tcgatgcagt aaaagcaatc
     3961 aaaatcgtta ctaatgcggg atgctcatgg acgggtgtca aactaacaaa gaaaaaaatc
     4021 actgcttcat aatatagaac aaacaagcgc cagaaatgtt tttctttttt tctaaaataa
     4081 tcaattagat actttgttag aagaatccct aagaaaaaga aaagggtaga caatactttt
     4141 ttaccaacac ttagccattc tccgtcaaat gcttggatga tcgatagaat gatattgcca
     4201 gtttgagcag aggcaaaagc accatattgg atataagtat aactgtccat ggctcctccg
     4261 ataaatgtca gcaaaagtcc tattgtacgc ccctcgtgaa aggaagtctg catttttttc
     4321 atcacctttt atgaatattt ttgtcttcct tatcttagca taaacgattg aaaaaaagga
     4381 aaaattttca gaaaactaaa atcatatcgc ttcccatttt catttttaga aaggtctgtt
     4441 agtataaaga aaaaatagac aacgaaactt taaataacca acttgaaata aacaaattca
     4501 aagaagaaag atgggaagga aatcaatcag ttgaatgaaa aagaaattct ccgattacaa
     4561 aaagaattga aacgctggga gaacaagtat aaaaagcttc aacaagtctt tcggctacaa
     4621 gaagaaatca ttgctaccta tgaagcggct tatggcgaag cacccggact tattgaggaa
     4681 gaagtacaag taaaagaaag tgaaggaaac agctctttct ttaaaatgat ggagaaacgt
     4741 tcctataatc gcttcaatca agaacaaaaa gcagctatca catatgatat gaagaaacat
     4801 cttcgaatca tagcaggagc tgggagcggc aaaacgcaaa caatctgtgc aaaagctgtt
     4861 tatctgatga cgcaaaaaca agtagacgaa gaacgcatac taatgatcac ttttacaagg
     4921 aacgctgcaa atgaactaaa aaaacgagta gataatttca gccagagaaa aaccgctgtc
     4981 catatcggaa cattccatag tatctttttt aggatataca atgaaatttg tcgaaaattt
     5041 cctgaagtag caatgcaagg aatacaagga gacttttcaa aggatactgc acaaaaagtg
     5101 aatgctgttt tgcaacagct gatacgtaaa tataatcttt atatatttga tcaatatgga
     5161 gaaaaaacga ttgcttctcg gttagactat tggcagaata tgaacctttc agtagaagaa
     5221 atggttcagt tagttaaaga aaaatatgat tccattgata agaatgcacg acagccgata
     5281 tcagaacgac tgtatggatt gctcacggaa ctccaagaac aaaaaagaag ccagcaattg
     5341 ctagagttca atgatgtact ccaaaatctg aaaagcgctt tggagaatga ggagatccag
     5401 cattatatcg gccaaaaata tgattatcta ttcattgatg aatttcaaga tactaatcca
     5461 cttcaatggg aaatcgtgaa acggatgaca aaggaaaaca ggatcaagtt gattgttgtt
     5521 ggagatgatg atcagagtat ctatggattc agaggatcag agccagcata catcaagaac
     5581 tttgaaaaag aatatcctac caaaactctt ttcttattaa ctaattatcg ttctcgggca
     5641 gctattgtcc aaggagcaaa tagattgatc agctataata aaaatgatcg gataccaaag
     5701 gcgatgattc cagcacaaaa agaagcaggt gttatggaag cctatttgtt ttctgatact
     5761 aaagcagaaa gtcagtggct aatcaaacaa attcagacat ttattcaaaa aaatgggaaa
     5821 tataaggaat caatcatatt ataccgatca gcttcacaaa cgcagcagct tgtccaatct
     5881 ctgctgaaaa cgaatattcc ttttgtactg gaagcagata gtccatatga aggtatcttt
     5941 ggtatcaagt ctttccaaca tttctatcaa aaaattgttc attggcaaac agcactgaat
     6001 ctgcaaaaga aaaatcaggc ctaccagcaa ttgctccggc agctgatggc agactgctac
     6061 ctgaaaaaaa gtgaatgcga tcaatacttt tctcctaaat atcaaaatat agccgttgca
     6121 gattatattt tgtcgataag ccccaattta aaatcgaaat ctgcagaaat ccgagagttt
     6181 gaggaagcgt tgcgtaaaac agaccagcat caagaaatct attcgcttgt tcaaagctat
     6241 cttcatctgc cacgtatagc taaggaaatc gaccagagtg atcaagaatg gatacgagaa
     6301 gaagtaaagc agcatgctac tttcagaaga ctccaagcct tatcgcaaga aacccaagcg
     6361 acagctaaag aattaaaaac ccgcttgatt gcttacagac aaggaaaact tgatgcacta
     6421 tgtatccaga gcattcacaa aagtaaaggt cttagttacc gaaatgtctt tttgattggt
     6481 tgtaacgaag gaagtttgcc ttataacgga gcgacagaaa agaaagtaat cgactttaat
     6541 gaaataaaag cagaaccagt aacgacgata gaagaagaac gaagactatt ttatgttgcg
     6601 atgactagag caagaaatcg tctgtatcta tgtgtacctc aaatgaaaaa ttcaagaaaa
     6661 ttgaaagtat cgagatttat caaagaaacc gggagtcgtg tgaaaaaagg aaagtgatag
     6721 aagagagggt gtgacagaag tggtcagttt caagaaagaa gaaggaatgt acgaaaattg
     6781 cttctcaaat ttttgtgaaa tttcagctta ttttcgaaga agctatttct ggaacacagt
     6841 ttattcggaa aagggaggtg tgacaagaat tttgtcacac ctcccttatt tattttgaaa
     6901 atgagaatac tattatcaaa atagagaata tatagaaaaa ttctcattat tcaaacgtaa
     6961 ataatcagtt attttgattt tttttatatt ttattagcta ataattggta ttttaaaaga
     7021 aattactgta gaattggatt ataattaaaa aggagtgaga aaatgataaa agtatatact
     7081 tcgcctagct gtacttcctg tagaaaagca agagcatggt tgcaagcgaa tcaacttgaa
     7141 tttgaagaga agaatatttt tgccgaaccg ttaacagaaa gtgagatcaa aaagatcttg
     7201 gcagcgactg aaggaggagt tcaagatatt atttccactc ggtcgaaaat ttacgaaaag
     7261 ctgggcatag attttaacga attaacttta aagcaaatga tcgtcttatt caaagaatat
     7321 ccttcattat tgagacgacc tattttgatt gatgaaaaaa gaatactaat cggatttaat
     7381 gaagatgaga tccggtcatt tgtccctcgt gaaattcgat gtgttgccag aaatctgatc
     7441 tatttacaaa acaataacat cttaaattca tagggaggga gccttcgtgg aagtgaaagt
     7501 ctatggatca gcgcactgtg taacaagtct gaaggcgaaa caatggttag aacaccatca
     7561 attgccttat caatttgttg acttagaaga acgcgatcta agtacagaag aagcagaaga
     7621 attatgcagt tttgaagaag tgcaagcaga acagctattt gcgacttggt ctgaggcttt
     7681 tcacaacatt gcgattgacc tttcctgtgt aggaaaaaaa caactgattt ttttatgcac
     7741 acaacatacc aatctcctca gacgtccttt gattattatc gatgaagttc tatttattgg
     7801 atacaatgag cagttgatgg aacagcttat cttaaataaa tagactttaa ctgttttaat
     7861 caagtcactg agcatttcat acagttgtaa cagtcgaaca cttagcatca agcgtgttat
     7921 gtgttctttt tttgacttta tttttctact atagagacat gctcaattta tgtggtcaaa
     7981 attcaaatct aggtggggat atagagttaa aaatatattg acatgtgtaa atttacatga
     8041 tatattcatt tctgttgttt taagtagcaa agcaataaaa tagtatttta aaaaacatat
     8101 aaaatagttg ttgacatgtt agaaatgaca tgataagata actgagttgt taaaaaagaa
     8161 ccacttgtga agcttgattc tatcgaaaaa aacttcaaaa aagttcttga caaataacaa
     8221 atgataagtt atgataaatg agttgctgta agatttcgaa aaacgaaatc aaaaaaactt
     8281 ttaaaaaaga gttgacaatt acttgtctct ctgatatgat ataaaagttg ctacggcagc
     8341 caaaaacgaa tcggggaaca ctcctgatga agtagacctt tgaaaactga acaaagtaag
     8401 acaaaccaaa tgtgtagggc gtcttgattc aattcaagac aacaaaca
//
LOCUS       DAZKNH010000107         8366 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000107 DAZKNH010000000
VERSION     DAZKNH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.107"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..1464)
                     /locus_tag="ACG8MG_002320"
                     /pseudo
     CDS             complement(38..1464)
                     /locus_tag="ACG8MG_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612187.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1707..2162
                     /gene="tnpA"
                     /locus_tag="ACG8MG_002321"
     CDS             1707..2162
                     /gene="tnpA"
                     /locus_tag="ACG8MG_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFQ0376330.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2427..2525
                     /locus_tag="ACG8MG_002322"
                     /pseudo
     CDS             2427..2525
                     /locus_tag="ACG8MG_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2748..2978)
                     /locus_tag="ACG8MG_002323"
     CDS             complement(2748..2978)
                     /locus_tag="ACG8MG_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFQ0376331.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(3208..4026)
                     /locus_tag="ACG8MG_002324"
     CDS             complement(3208..4026)
                     /locus_tag="ACG8MG_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0376332.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            4187..4876
                     /locus_tag="ACG8MG_002325"
     CDS             4187..4876
                     /locus_tag="ACG8MG_002325"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0376333.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4890..6392
                     /locus_tag="ACG8MG_002326"
     CDS             4890..6392
                     /locus_tag="ACG8MG_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0376334.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6405..6881
                     /locus_tag="ACG8MG_002327"
     CDS             6405..6881
                     /locus_tag="ACG8MG_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0376335.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6978..7088)
                     /locus_tag="ACG8MG_002328"
                     /pseudo
     CDS             complement(6978..7088)
                     /locus_tag="ACG8MG_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7379..8366)
                     /locus_tag="ACG8MG_002329"
                     /pseudo
     CDS             complement(7379..8366)
                     /locus_tag="ACG8MG_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT         2642 a         1371 c         1512 g         2841 t
ORIGIN      
        1 aaatccactc cttttttcat gctctaaaat gatattttta cacaactgtt acggttgttt
       61 tacgaaattg gacgggactt tgccagttta atttctgttt tatccgttca ttattgtagt
      121 aatagatata gctatcgatg gctgatttta attcctcgaa acagttataa actttcccgt
      181 gaaaaatctc ttgttttaat aagccaaaaa aattctccat aagtgaatta tctaagcagt
      241 tccctttccg agacatgctt tgaaagattt tgtactcctt caatttgttt ctataagctt
      301 tcatttgata cgcccaacct tgatctgagt gaaacgtacg acgataggga caatcgttag
      361 tggtctggat agcttcctcc aaaccttcca ttaccgctaa tgcattgggc ttttcagaga
      421 tacgatagga taaaatctca ctattgaaca tatccataaa aggatctaga tacaatttct
      481 tttgacggat aatgcctttg gcatccactt catggtattt aaattcagtt gtatccgtcg
      541 tgattttctg atggcagata ctggtgtaga aacgacgatg gatccgattt ttggctactg
      601 ctccgacttg tcctcgataa gaattgtagc gccgtgattt acgtgtgtat gtcgttactt
      661 ttattcctag cttcttgatc aggcgctgaa ctttcttttt attgatagag aaaccgcggt
      721 ttcttagttc attggtcatc cgtaaacagc cataatcctt gtgcttctcc cgaactttaa
      781 gcatttccgc ttcaatctct tgatctggat ttttacggtc caatcgtttt tgccagtaca
      841 taaagattgc cttagggaac tttaaagttt ctagaagctc aactaattta aacggtcctc
      901 ggaggcttgc gatgattcgt gcgattgctt cattcgtcgt tgttgggctt cctgttttcg
      961 cagcttcctc aattctttta aaaaggcatt ttcaatttga agggaacgga ctcgtttctc
     1021 caactcctcg atgcgttcac gttctttaga cgttgcttta gttgtagatt ttttcttatt
     1081 tttgggttca tttttcttag acaagatagg tggacatcct ttcgtttttg agagtccatc
     1141 gatcccaccc tcacggaatt gacgcatcca actagcgatc aaattagggt tgttcatgtt
     1201 aagtgtgttg gcaacttcac gataggacag ctcacttgtt agatataact ctatcgcatc
     1261 cagcttaaat tgaacagaat attttttctt ttgtcgcttt cttactagac cctcttcacc
     1321 aaattcttga taggcattga tccagcgctt cacttgtgat ggagccttaa tggaatattt
     1381 ctttgccaga taatcacttc cgcctttacc atccaagtaa tcatgaacga tctttaattt
     1441 aagttcaaaa gtatacttag ccataaaaaa taccgacctc caaaagttag attttttggt
     1501 ctaacttttg ggggtcggta caaaacttaa aagttttaga cccttttatt ttcttaatga
     1561 cttcagtcag ttttgtgcgt aaagcgtgcg aaacaagaaa accgcgcgtt tctacgcgtg
     1621 gaagaagtat ctttagcgat aaaaactcct ttcgatatac tagtgagtgg ctaccaaccg
     1681 ctcatcagat cgaaaggagt tttttcatgt caaatgacga taaaagttta gcatatactc
     1741 gttggaattg taagtatcat attattttta caccaaaata tcaacgaaaa gtaatctatg
     1801 ggcaattacg aaaagatctt ggaaaaatac ttaggaagtt gtgcgaaatg aaagatgtgg
     1861 aaatcataga agcacatgca atgctagatc atattcacat gcttgttcga gtaccaccaa
     1921 aaatatgtat atccagtttt atggggtatc tgaaaggtcg tagtgcggta ctaatccacg
     1981 agcagcacgc aaatctcaaa tataaatacg gaaacaaatc cttttggtcg aagagatact
     2041 atgtgagtac agtcggattg aatcaaaaga caaatgaaaa atacatccgt gaacaagaag
     2101 ctgatgatcg cattcgatat attataagta aaagagagta cgtagaacca tttaaaaaat
     2161 aaagagaagt aagtgagtga agatgagcag ttggcggtct tttaaaaatc cccttttaag
     2221 gggcagcgag tgtcgacccc ttctaggggg attaaaaata cacccgtcct cacgggtgta
     2281 tgggttactg atagacttga ttttaactgg tgtcagaaac ttatgtccag aaagggtgat
     2341 gaaattttgg caatttacgg ctattcaaga attagcacaa gcaaccaaga ttacaaaaca
     2401 caaattcaaa cattagaaaa tgctggcgca gaaataatct tcctagaaaa tcactggatt
     2461 atcgaacacc tttggaagta tttttgagtt acgtaagtat tgatgatctg tctaacttaa
     2521 tttgacaatt aagagtatat ttacttcaga tggtaaggca acaaaataaa atacaatagg
     2581 cagatgaact aataaataaa atctgtatta aatacagata aattaatata aaaatcaaaa
     2641 ggatcataaa cttttaacaa gtgatgaaat gggtggaaat tgattcttat agacagtttg
     2701 tctcataaca ttgaagtatt ttgcaatttt agcattgaaa cactttttta atcttcaacg
     2761 ttgttcttat aaacaagatg attgggaatc ttttgaatta acactgtata gcttagaaca
     2821 ccctttatag aaaacattcg cttagcaatc ttaataattc cttgtgcccg atgtcgttta
     2881 gattcagtag gttcctgctc atcgatcatg gaaaaaattt gaaggatcag atccttaata
     2941 aatttattta agagaggatg atccattgct tcatccatac tcgataaact agtgataatg
     3001 agctacacgt ttttctttca agttgatcaa tagcttccac gataaaaata tctttagata
     3061 aaatcaagaa ccgtaggaac tacagggata tttggtcaat aagagccacc gctattagta
     3121 aaaaataagc tatcaagtat gctctcatcc caagaattgc ctacttcaat cgttaaaaac
     3181 cgttaggttt agctaagtgg gggtagttca ctcaatactt ttatcaataa tagagcgcaa
     3241 aactttttct cttaattcaa ctgaatcaat attattgttt tgttcataac catgaactaa
     3301 tagatcacaa atataaagtt gtgatatttt tccagctaaa gatccaccat taagaaattc
     3361 ttcaatggct gtttgtaaaa ctaagtctgc agactttcct ataggtgaat gaatatagtt
     3421 agttatagca ataatttttg cgccattttt tttagctatt ttcaatgaat cataagtatc
     3481 tttcgttttc ccagataaag aaaaaatgat tactaaatca ttaactgtta ataaagaagc
     3541 gacttgggct tgaaaatgag ggtctagtac agcttttgct tgaactccta cgcgaagaaa
     3601 catattttct aaatcaagac tagtatttcc actagatccc actccaaata tatatatgtt
     3661 ttttgattga gtgatcattt taatggcttc attcaatttt tcttcgtcta aaagcctaat
     3721 agtggaatag agagcttctg ttaaactatt agcaatttca tcatagtact ttacactagt
     3781 cggatgttct tttttttgac taaagtcttc ctttgcaatt tcaattttta aatcagaaaa
     3841 accagaaaaa ccaatttttt gacaaaagcg aataatagta gcatcgccaa cttttgtgtt
     3901 tttcttaata tcactcatcg tactataaat aatagttttc ccagaattta aaataaaatc
     3961 agcgactttt ttttcagact tcgttaaaaa tggataattt tctttaatta aatagatata
     4021 actcatacaa actcctctac aatttcaact tttctccatt atactccaaa ataataaaaa
     4081 aaactcctaa atactccatt tttatcttta cttttatgaa agcggatgct atattgtttt
     4141 tggagttatt atccaaaaat aataaaaaat tggagcgttt aggataatgg ataaaaagag
     4201 gtttttagat cttatttcaa aaggtttgat tgtttcatgt caagcattac ctggtgaacc
     4261 attttacaaa gaggaagaaa gtttaatgcc ttattttgct ttggctgctc aaagggctgg
     4321 agcagttggt atacgtgcaa attctgttcg agatattatt gaaattagaa agtgtgtatc
     4381 attaccaatg attggaatta ttaaaagaga ttatccacca gaatctccat tcattactgc
     4441 cactatgaga gaagtagatg aattagtaga aaccggtgtt gaggtaatcg ctcttgattg
     4501 cactcttaga aatcgacatg atggactatc tataaattct tttattacta aaataaaaga
     4561 aaaatatcct catcagttat ttatggcaga tatttctaca tttgaagaag gcaaaaatgc
     4621 atataaagca ggaatagatt ttgtaggaac tactttaagt ggatatactg aggaaagtaa
     4681 aaaacaagat gggccagata tagatttgat taaaaaattg gtagaagaag ggatttgtgt
     4741 aattgctgag ggaaggattc atactccaga acaagcaaaa gagataaaaa aaataggagt
     4801 tgcaggtatt gttgtgggtg gtgctattac tcgaccacag gaaattgcat caagatttgt
     4861 taaagcatta agttaggggg agaaataaaa tgtttaaaaa attttcacaa ttaggacgcg
     4921 ctttcatgtt accaattgct atattaccag tagccggatt actattggga ctgggaggag
     4981 cgttaacaaa tgagagtgct ataaatgctt atccattttt agatcaaccg tggttgcata
     5041 caattttaag tattatgagt tatgctggaa atgcagtttt tgcaaaccta gcactaattt
     5101 ttgcaattgg tattgctgta ggacttgcaa atggagataa aggaacagct ggtttagctg
     5161 gtggagtagc ttatctagta tatactgcaa ctattagtgg gtttcttgaa cttttttcag
     5221 caaaagatgc aacactagat acaggtgtag tgggatcaat tgttgttggt ggtactgttg
     5281 catttttaca caatcgttat agaaaaattg aattaccaca attcttaggt ttttttggag
     5341 gatcaagatt tattccgatt gtttctagtt tagctgcaat cattattggt tctttctttt
     5401 atttaatttg gccaccaatt caaaatggtt tagtagttgc tggtgaacaa attgcccaaa
     5461 tgggtagttt aggaactttt ttatatggtt ttttattgcg tttaacaggt gctgttggac
     5521 tacaccacac catttaccct ttgttctggt atacatcttt aggtggtacc gaaactgtag
     5581 ctggatcaac aattgctgga gcccaaaata ttttctttgc ccaactagct gatccaaacc
     5641 acactggtct gtttacttat ggtactagat tttttgcagg aagatttgca acaatgatgt
     5701 ttggattacc agcagcatgt tatgcaatgt atcgtgcaat tcctaagaaa aatcgtaaaa
     5761 agaatggtgg gctttatttt agcggggccc ttacatcatt tttaactgga attactgaac
     5821 cagttgaata tatgttttta tttgttgctc cttggcttta tgtgattcat gcattcttag
     5881 atggattatc tttttatttt gctgatatct taaatattcg tgtagggaat tccttttcag
     5941 gtggtttgat cgacttttta ttattcggtg tacttcaagg gaatgacaaa actaattggt
     6001 taaaattaat tccttttggt attgcatggg catttatata tttctttgtt tttacattct
     6061 ttatcaaaaa atttaaggta gctattcctg gaatggaaga agaatcagaa gttcattctg
     6121 aaaaagtaat aaacaaaaat acatcattac atgaagagtc tcttatcatt attgaagctc
     6181 ttggtggaaa agaaaatatt gagtctgttg aagcttgtgc aactcgtttg cgtgttgctg
     6241 ttacgaatgg ggagattatt caaaaacaaa caataaaaga cactggtgca actgctgtct
     6301 tcgaagtgaa gggtggcatc caagctgttt ttggagggaa agctgactta ttaagtcaag
     6361 aaatcaatca aatattagga aaagataatt aggaggtttt taatatgttt gaaattttca
     6421 aaaagaaaaa acaagaaact ttttatgcac cttgttccgg aaaaatcatt tccatcacag
     6481 aagtaaatga tcctgtcttt tcacaaaaat tactaggtga tggttttgcc gtcatacctg
     6541 atggttcaaa aatttatgct ccattggctg gtcgagtaac aaatatattt cctactaaac
     6601 atgctattaa tataaaaagc aactcaggca ttgattattt gattcatatt ggaattgata
     6661 cagttgaatt acaaggagag cctttttcga tttgtgttca agaaaatgat gaggttactc
     6721 ctgaaacctc gttggttgat gtggattttg acaaaataat aaaatctggt aaagatactt
     6781 ctgttatagt tgtttttccg gagaaaaatc agttcaatga tttaataata gataatggtc
     6841 gaattgctca tgggaattca tgtggaaaaa tcacttttta aaaaagtgta tataaaaagc
     6901 tataaaataa gctaaatagg atatttgtca aatgaagttg gtgaacagaa agaactgttt
     6961 tctttctgtt cattttttta ttttattagt aatgattccc catgccaaat catgatccgt
     7021 aatttaaagt tataaaagct tcgaaatcca ttggcatttc gttggaatac cttgatatga
     7081 ttattcaaat ataaaatata gagagaagag agagagggaa gatatttata aagggcaaag
     7141 ttatgtttac tcaacttagg aaatagaaat agatcaattt atattctctt gtcaatcgaa
     7201 tatgaacctt agcactatat accaaatata gtaaaatgct gaaagatgta tggaatggca
     7261 acagtttttt tgacaaattt tataaggtgc agaacttctt tccgtatgct attccgattg
     7321 tccttgacaa tgagcctcct cggatgtgac gatcttcggc aggagctaac agttaaagtt
     7381 agactgcctc gctagcgtta gcacatccga gctcattatc aaggattcgc tgcgccaatc
     7441 tctggttacg gtaaccaacc ggtgtctcgt agccaagtgt accgtgcaac cgaaggtggt
     7501 tccaccaatt gacatagtca aataactcca aatccaattg ttgtaaggtt tcaaatgtgt
     7561 attgatagac aaattctact ttcaacgact tataagttga ttcagctacg gcattatcaa
     7621 aaggacagcc tttatgactc aatgatcgat tgatgtcaaa agttgttaat aattcatcaa
     7681 tagcttggtt atcaaactct tttccacgat cagtatgaaa aatctcaacc tctgtcagag
     7741 gttgtttgat acggctaaat gcttttttta ctagaacggc atctttatgt tctccacaag
     7801 aatagccgag aatttctcga ttgaacagat ccaaaatgaa acagacataa ttccattttt
     7861 tcccgactcg tacataagtc aagtctgtta cgatcgcttc taatgggttg tctcttaaga
     7921 atttacgatt caatacgttt gtcgttttgg cttcattgca agtagaatga tgtactttaa
     7981 aataagcaac agtatagctc gattttaatc ctctattttt catgattcta ctaatttttc
     8041 gtcggctgat ctgaatgcct cgttttgata aggctttttt tatttttctt gagccgtagg
     8101 cctttcggct gcggataaat tcttcagcga ctacttcttc aagttctgat tcgtctttct
     8161 ttggttttga ttgataataa taggactgac gtgatagacc taatattctg cacatcgctg
     8221 atatagggta aagatgctta ttcgcatcga ttacttgtct cttcgtccga atatcagcgc
     8281 tgcttgcttt aaaatatcat tttccatttc taattgctgg tttcttttac gtagttctaa
     8341 caattctttt tgttcaggcg taagat
//
LOCUS       DAZKNH010000108         8000 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000108 DAZKNH010000000
VERSION     DAZKNH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.108"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            253..954
                     /locus_tag="ACG8MG_002330"
     CDS             253..954
                     /locus_tag="ACG8MG_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0376336.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1563..2579)
                     /gene="tsaD"
                     /locus_tag="ACG8MG_002331"
     CDS             complement(1563..2579)
                     /gene="tsaD"
                     /locus_tag="ACG8MG_002331"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFQ0376337.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2576..3040)
                     /gene="rimI"
                     /locus_tag="ACG8MG_002332"
     CDS             complement(2576..3040)
                     /gene="rimI"
                     /locus_tag="ACG8MG_002332"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0376338.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(3047..3589)
                     /gene="rimI"
                     /locus_tag="ACG8MG_002333"
     CDS             complement(3047..3589)
                     /gene="rimI"
                     /locus_tag="ACG8MG_002333"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0376339.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3573..4295)
                     /gene="tsaB"
                     /locus_tag="ACG8MG_002334"
     CDS             complement(3573..4295)
                     /gene="tsaB"
                     /locus_tag="ACG8MG_002334"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFQ0376340.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4486..5466)
                     /locus_tag="ACG8MG_002335"
     CDS             complement(4486..5466)
                     /locus_tag="ACG8MG_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0376341.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5490..6974)
                     /gene="galT"
                     /locus_tag="ACG8MG_002336"
     CDS             complement(5490..6974)
                     /gene="galT"
                     /locus_tag="ACG8MG_002336"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFQ0376342.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6986..7975)
                     /gene="galE"
                     /locus_tag="ACG8MG_002337"
     CDS             complement(6986..7975)
                     /gene="galE"
                     /locus_tag="ACG8MG_002337"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFQ0376343.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2255 a         1710 c         1403 g         2632 t
ORIGIN      
        1 tttttgaata aattaaaaaa ataaattata ttatcgttgt tagcgtttac tatatgtatt
       61 attgttgttt tttatacaca aatcattcgc ttcaaaacaa attattcaat accattgtat
      121 attttttaca taataacaaa ttatatttgt aaagtattaa aacttgtttc taatgttggt
      181 atgttaaaac tttacaaatt ctttaaaaaa agtgacaata aatatagtga acaataagga
      241 gaggactttt taatgcgtgt taataaaatc gattttcact tttcaaaact cagatatcaa
      301 cctgattttg aacatttctc ggatgaagag ttccaacaga tcaaggaaca tactgttctt
      361 cgttcttata aaaaaggaca gatccttttc gacgatggtg atcccagaca acggttgtat
      421 atcttgacca aaggactcgt tcgtttagaa cgttatgatg aaagtgcaac ttattactac
      481 tatgactatg tgaatacaaa taaccttttt cctatgtcag gaatgtttgg agaagagacc
      541 tacacttata cagctcaagc gatgactgat attgaaacat tctaccttcc tgtttcctac
      601 tatgaaaaat tagcacgaaa aaatcccgaa cagttgatct atatgataca aaaaatttct
      661 aaagtagttg aaatacatga attgcgtatc caaatcggat tgacctctag cgcctttgac
      721 cgggtaaaac aaaatttatt cattttgaaa gaagaattgg gcgtaccaac aaaagatggt
      781 catatcacca ttccttatcc gatcacctta aaagaattag cagtgaatag cggcacgaca
      841 cgtgaaactg ctggacaagt aatcaaacaa ctacgtgatt ccgggctgtt agattatgac
      901 aaaaagcgtt ttactttctg taagacagct actactccag ctatgagaga ataacgcgaa
      961 caaaaataag cagtgaccct gataaatatc agggtcactg cttattttcc tccctaaaac
     1021 ttctgaaaga tccggcagtt ttagatcact ggacaagact caaagatttt ctatgtgtgt
     1081 ttcagttatg taatattcac tgtttgcaaa aaatactgtt agattaacta gatgaagcgt
     1141 cttgtcctca tccgatctag attattcgat attcctcgac aataaacgat tactcagtga
     1201 gggcaatcag aggaacggat aatgataaat aagctgtaaa aaatagcaaa ataacaataa
     1261 tgattaaaag tatgtaaaaa atagggcttt gacaaaaacg tgtcacagcc ctatttcgat
     1321 taaacggtgt gtacctgtca actcttcggt cacaagtcat aatgttcata aacatgagga
     1381 gctgtttttt gaaattgttc cttgtgaatc agagttaaac gacaggctta cgaactcttg
     1441 attaaacggc gttcaaaagc ttgcatacgc ggtaataaga acagccaggt caaaaacatg
     1501 agaagccgtt tttcctgggc tgttcttatt acttgatgca gggcagcttt ttcacaacct
     1561 ctttattcag ataaaaccaa gcttggatca gcattcaact gatagctagc aaaatttctt
     1621 ttttgcattt ccacatgtgc tgctgctcca atcattgcag cattatctcc acaaagtcga
     1681 agagggggaa tcactagttc tacctcaggg agtttagctg ccaaagcatc ctgcaatcct
     1741 tcacgcaacc cttgattggc cgctacacct ccagctataa tcagttgctt gactggataa
     1801 ttttgacaag ctcgaagtgt tttagtaatc aatacatcga tcacacttgc ttgaaagcta
     1861 gctgctaaat catttttgtc tagatcttct ccacgctgtt gtgcattatg caccaaattg
     1921 ataaatgcac tttttaatcc gctaaaactg aaatcataat tatcttcatg gatcatcgct
     1981 cgcggaaaat ggtaattatc ttttccctga tgagctagct gatcgatttc ttttccgctt
     2041 gggtaagaaa gacctaatac tcttccaact ttgtcataag cttctcctgc tgcgtcatct
     2101 cttgtctccc caatgatttc atatgatcca tcttcttgca tatagactaa ttctgtatgt
     2161 cctccgctaa caagaagtgc catcaatgga aactggaatg gtttaaccag acgggctgca
     2221 tagatatgcc ctgccatatg gtttacagga ataagcggca attggtgtgc ccaagcaaaa
     2281 gccttagctg cagaaatacc aatcagcaac gagccgacaa gtcctggtcc ataagtgaca
     2341 gcgactgccg ataaatcttc tgccgacaca ccagcttcta ccagtgcatc ttccaagcat
     2401 aaggtgattt gttctacgtg atggcggcta gctacttcag gtactacgcc accaaaacgt
     2461 ttatgactgt ttatttgaga agcaacgatg ttagataaga tctctgttcc atttctaacg
     2521 accgctacac tcgtttcatc acaacttgat tcaatcgcca gaatcaattc ttcattcatt
     2581 tcatcgtctc cgtttttaac tttctgctca taatgatcgc atcttccgtc ggattgcgat
     2641 aatatgattt ccgtttacct aatatacgaa acttttctga ttcataaagt tttcttgctg
     2701 cttcattaga tagacgtact tctaaaaaaa catgcagcga atcttttatt tgttccgtaa
     2761 tcagtaattg taacaaatgc cgtgcatgcc cttttccttg ttctgaaact gcaacagcaa
     2821 tatttgtgat ctcgacttca tctattactt tcgagtatcc aataaagcca cagatctggt
     2881 tatcttcagc taaaagcaga taatcagagt gaggctgttg gatgtctgcc aaaaactgca
     2941 ttttcgtcca aggtgaacca tgtggataag cattttcgct aatcatccat acgcattctg
     3001 ccaagtacgc ttcatcaaaa tctttttttc tgcaaatcaa ctaatctcac tcattttcta
     3061 aaagttttat catatccaac gcatcttctt ttgtttcagt gtaatatctt tttttgatcg
     3121 ttgttgcttg aaaccccaat ttccgataaa gtctttgtgc atcttgatta ctcatacgca
     3181 cttctaatga catcgtctca cagcgattca ttatagcaaa gttctcgatt tcgtcaataa
     3241 gcagcgaacc gattcgttta cgctgatatg ccggtcgaac ggcaatattc gtgatatggc
     3301 aatctaaacc gaacgctctt gaaccgataa aaccaatcat tcttccatta tcaaagatac
     3361 ctagatatag atgaggaagc ggtgacttca attctgatat aaaggcactt tttgtccatg
     3421 gcaattcgcc attatagact tctcgttcga catccagcag ctctttgata tcttctgtag
     3481 tcaattctct tagtacgtaa cgacgatcga tttgttcgac tgttttttct gtataaatca
     3541 ttttatcttg aaaaaagctt ctggcatacg ctctaaattt tttcaacata actttcgtct
     3601 ccaggtgtgt gcgtctccaa ccatttttct tctgcttcta ctcgttttaa atagcgagga
     3661 agaaaagctt gtttctctgc aactggttca actaaagcac ctaattgagc cacagttaca
     3721 ccacttggaa tatcccatgc tggaactgta ttaatcatcg cactaggcaa tatttccttt
     3781 atttcagaga agaatttgtg acaatcactc ccgacgaaat aaatagcttc attcagcttt
     3841 tgtagttgtg tgaacaaatc agaagcagaa atatgtacat cttcaagtac agtttctaat
     3901 ctattttctt tccagcggta tgccccagcg tatacatttt ttcttcgagc atcaaacatg
     3961 ggcacaataa tattatccac accgatgcaa ttagccgcga tggttttcag actagatact
     4021 cctactagct ctttatctaa tgtataagca atcgtttttg ctgtcgttac acctatacga
     4081 agccctgtat aagaaccggg accatcagaa accgcgattc ggtcaatgtc ttttggactt
     4141 atttgaagat ctgcaaacaa ttggtcgatt gcaggcatca gtgttacact gtggttgcgt
     4201 ttgatatttg tttgtatttg tccaagaatt tgttcgtctt ctaccacacc gatcgccaaa
     4261 gtttggtttg cagtatctat tcctaatgta atcatatatt tgatcctttc tgctatgaca
     4321 ttctcgcctt ccattgtagc atactttttt attctgagga tgaaaatacc aaaggcaaaa
     4381 ctagtacaat aatacatgat tatctgctgg cgggcaaatt ttgccccttc ctgaaacaca
     4441 aaaaagctgc ggtatcactc cattttccca cagcttttcc atcttttatt tcgttgattc
     4501 tcgaataatc aactctgttt ccacaataat ctttcttgaa accggtgctt tggaattact
     4561 taattgaaga atcgtctcca ctgctagctc tcccatccat tcggtataaa cttttacagt
     4621 agaaagcgag ggcgttacat atttcgctac actgacatca ttgaagccca tcacagaaat
     4681 atcttctggt accactaaac ctttttcttg aatcgctttc agtgcgccaa tcgctaatgc
     4741 atcattagct gcaaaaagcg catcaggaac atgcggattc gtttttaaat attgcttcac
     4801 tgcttgatat cctgactcga cagaaaaatc tgcttcgata attggacctt cgtcgtagcc
     4861 tttttctgcc aaaacttgtt gaaatgccat aattctggga tcttttagcc gctgatggtt
     4921 tttcttcgta tattctgttc cagcaagcat agcgatatgt tgcgctttat gggagacaaa
     4981 gtgttcaatg actttttgaa cacttgactt aaagtcgaca actagagagt cgattccttg
     5041 agaagtgccg tcagaatcta ccaaaagcaa ggcattttgg gatcctttca tttctaataa
     5101 ctgagcttcg tcgaattttc ctagtgcgat cgttccatct gccggaagat cactaatgga
     5161 atcccacgtt tctttcaata aagtaacgtt tgcttcttct gccttttttt cgattcctag
     5221 gcgaatagat aaataataaa gatcttcaag ctcttccgta tcgtcgtacc actggatcag
     5281 gcgaagcgtt tgcttagtat ttgctgcatt tttacgatgt ttcgtataat ttaagcgttc
     5341 tgctgcttca aaaatttttt gctttgtggc taagccaacg gaaagatcag gatcatagtt
     5401 gagaactctt gaaacagtag caggagaaac tcctgccaac tgcgcaatgt ctttaattgt
     5461 tgccatttgt ctctcctcct tttttacgtt tataaaatcg cagtaaatcg ttcaaacgct
     5521 gcctgtcctt tttcatctcg cttgaatact ccagcgtctt ccagtacacg tgcaaaaacc
     5581 tgacctacag atttttgtac gacatcggtg accgtatctt tcgtcaaatc aggatgttct
     5641 ttttttaatt gttctgccca cggacgatga tattcagcca tctggttttc ttctcctaat
     5701 aagaaatgct ctacttctac tagttctggt ttcaaacgag gtggtaaaac agcaagcccc
     5761 attacttcga tcaacccaat gttctccttt ttgatatgtt ggacatcttt atgtggatga
     5821 aaaatcccat caggatgttc ttctgaaaca ttgttgtctc gaaggacaag gtccaactca
     5881 aagtaattgt cccttcttcg tgcaattggc gtaatcgtat ggtgtggtgt accatctgct
     5941 gaaaaagctc tgatagatac tgtttcatcc gaatatgctt tccatttttc caatactgag
     6001 ttagctgctt caataagcaa gtttttctct gttgattgca aacggatcac agacatcggc
     6061 catttcacga ctcctgcgat gacttgcgga taatcttcta gtttgataag tttttctatc
     6121 ggtgcttttg ccataggaaa ttcatgacgt cctgcctgat aatgatcatg agaaagaatc
     6181 gatcctccaa caattggtaa gtcagcattc gaaccaacaa agtaatgagg caagacttca
     6241 gtaatccgaa gcagtctagc aaatgtttcc tttgtgattt tcatcgggcg gtgttgttct
     6301 gataaaatga tcgaatgttc attgtaataa gcataagggg aatattggaa tccccagctt
     6361 tcaccgtcta gattcattcg aataatccgg tgattggtgc gggctggata gttcaagcga
     6421 cctctatatc cttcgttttc catgcaaagc atgcaggtag gatagtctac ttttaccgcc
     6481 tcgcgttctg ctgcgatttg ttttggatct ttttctggct tagataaatt gattgtgatt
     6541 tccagttcac catactccga ctctgccgga aatttgatgt tttttgcgat ggctcttgtt
     6601 ttgatgtagt catttttctt acttaaatta aagaaatact cagttgcttc ctctgggctt
     6661 ttcgaatagt gctgtgcaaa gaatgcattg acgactgaag gtggcggtgt gagaaaatcc
     6721 attaattgcg cttcaaaaat ctcttgttct gctggcaatt ctgaaagaac accattttct
     6781 cttgccttag cgacaagctg atccaataag tctacagatt cttccttaac tggtcttact
     6841 tcaatgcttt ctagagcatc ttctcctacc atccctaaaa gacgattctg taaatataag
     6901 cggtccattt ccatccatcc gccagcttcg atcgcaagtg tggcaaaatc acaaatcgtt
     6961 tgactgattg acatatctca ttcccctatt cttcataacc atttggatgg ctgacatgcc
     7021 aatcccatgc tgttttgata atcgctttga tatctgtgta ttcaggtttc cagcccagaa
     7081 tctctctcgc tttttcactt gaagcgacta atcggctagg atctccagct cttcttggtg
     7141 ctatttctgc tggaatttct tttcctgtca cttctctagc tgcttctagc atttccttga
     7201 ctgagtatcc tttgctgctg cctaaattga agaagttgct ttcatttcct tctttcaagt
     7261 attccaaagc taaaatatgt gcagcaatca aatcttctac ttgaacataa tctctgatac
     7321 atgttccatc tggtgtatca taatcgtctc cataaacagc caaggctttt ctttgaccaa
     7381 gagctacttg taaaatgatt gggacaagat gcgtttctgg tgtatgatct tctccgatag
     7441 atgcatctgc ttttgctccg gctacattga agtagcgcaa agcgacatag cgcatgccat
     7501 atgcttggtc acaccacttc atcatttttt ccatcatcaa tttactttca ccatacggat
     7561 ttttaggatt agctggtgta tcttctgtga ttggagactc tttcggctct ccataagttg
     7621 ctgcagtaga agagaatacg attttattca cgttgaactc gtgcatgact tcaagtaaga
     7681 tttgcatacc gtatacgttg ttgttgaaat acatcaatgg tttttcgaca gattctccta
     7741 ctaaagagct tgcagcaaaa tggattactc cttcaatcgg ttccttttca aatacggaac
     7801 gtagaaattc tttatcccgg atgtctcctt cataaaaatg agcatctgga tgtactgctt
     7861 gtttgtgacc tgtgagcaaa ttatctacca cagctacttg ataccccttt tgaactagct
     7921 ggtctacagc gtgcgaaccg atataaccgg ctccgcctag tactaaaatt gtcataaaaa
     7981 acactccttt gattagtaaa
//
LOCUS       DAZKNH010000109         7537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000109 DAZKNH010000000
VERSION     DAZKNH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.109"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..1248
                     /locus_tag="ACG8MG_002338"
     CDS             join(107..335,335..1248)
                     /locus_tag="ACG8MG_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0376344.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="ACG8MG_002339"
     CDS             1483..1935
                     /locus_tag="ACG8MG_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376345.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="ACG8MG_002340"
     CDS             1949..2638
                     /locus_tag="ACG8MG_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376346.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="ACG8MG_002341"
     CDS             2763..5114
                     /locus_tag="ACG8MG_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFQ0376347.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="ACG8MG_002342"
     CDS             5194..5589
                     /locus_tag="ACG8MG_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376348.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="ACG8MG_002343"
     CDS             5914..6531
                     /locus_tag="ACG8MG_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0376349.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="ACG8MG_002344"
     CDS             6572..6871
                     /locus_tag="ACG8MG_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFQ0376350.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="ACG8MG_002345"
     CDS             6905..7072
                     /locus_tag="ACG8MG_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0376351.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="ACG8MG_002346"
     CDS             complement(7117..>7537)
                     /locus_tag="ACG8MG_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0376352.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DAZKNH010000110         7470 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000110 DAZKNH010000000
VERSION     DAZKNH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.110"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..931
                     /locus_tag="ACG8MG_002347"
     CDS             <1..931
                     /locus_tag="ACG8MG_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0376353.1"
                     /translation="ISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHF
                     FDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSV
                     IWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKD
                     YYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPR
                     LGIIAAGYTTVFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILIN
                     QMLIRYVLTSLLIISLVIYFLKIKNRIIDDYNS"
     gene            944..1951
                     /locus_tag="ACG8MG_002348"
     CDS             944..1951
                     /locus_tag="ACG8MG_002348"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFQ0376354.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1963..2967
                     /gene="neuB"
                     /locus_tag="ACG8MG_002349"
     CDS             1963..2967
                     /gene="neuB"
                     /locus_tag="ACG8MG_002349"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFQ0376355.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2964..4112
                     /gene="neuC"
                     /locus_tag="ACG8MG_002350"
     CDS             2964..4112
                     /gene="neuC"
                     /locus_tag="ACG8MG_002350"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFQ0376356.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            4085..4762
                     /locus_tag="ACG8MG_002351"
     CDS             4085..4762
                     /locus_tag="ACG8MG_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFQ0376357.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            4713..5894
                     /locus_tag="ACG8MG_002352"
     CDS             4713..5894
                     /locus_tag="ACG8MG_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFQ0376358.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            5907..6884
                     /locus_tag="ACG8MG_002353"
     CDS             5907..6884
                     /locus_tag="ACG8MG_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0376359.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            6884..>7470
                     /locus_tag="ACG8MG_002354"
     CDS             6884..>7470
                     /locus_tag="ACG8MG_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0376360.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYS"
BASE COUNT         2741 a          933 c         1404 g         2392 t
ORIGIN      
        1 aatctctgta gctttgatgc agacattata ttttgatgat aaatatgttg gcagagtagt
       61 cggcggaaca ctgtcatttg tgttgatagg tttagtaatt tatttaagaa taatgataaa
      121 gggtgctcat ttttttgata aaaaacagtg gagatacgct ttaaaaatct ctttgccgct
      181 aatcccacat tccttagcaa atatagtttt agcacaattc gatagaatta tgattaattc
      241 ttatttaggt cctgctgcgg ggggaatata ttcctacatt agtaacatag gtattattct
      301 aagcgtaata tggatgtcta ctaataacgc ctgggttcca tggttctatg gtgagatgag
      361 taaaaataat gaaaaaaaag tgaagaaagt tagtaactat tatttagtag tctttacttt
      421 gattacgatg attgtaatgg tagtttcgat tgatttagca aaatttatgg ctccgaaaga
      481 ctattattct gggttatctt tagtggttcc gataacctta ggctatttct ttcagttttt
      541 atatagttta ccagttaatg ctgaatttta tgagaaaaaa acatcgttta ttgcgattgg
      601 tacagtagca agtgctatag ttaatatact attaaacatg attttcatac ctaggttagg
      661 aataattgct gctggataca caaccgtatt tacgtatttt ttacttttta tttttcatta
      721 taatatcgct aaaaaaatta cgaataaaca attatttgac acaaaaaaaa tatgtatcat
      781 aataattttt gtatcaattt tttctttgat acttcatatt ttaatcaatc aaatgttgat
      841 tcgttacgtt ttgacatcat tattgataat tagtttagtt atatattttt taaagattaa
      901 gaatcgaatt atagatgact ataattcata ggaggagata taagtgttta aaggaaaaac
      961 aatattagta actggcggaa ctggttccat tggaagtgaa attgttcgtc agcttttaaa
     1021 atatgaacca aaagctatca gaatttatag tagaggtgaa gaaaaacaat tttatatgca
     1081 acaagagctc tcaaatcaca ctaatttgag atttttaatt ggtgatatca gagactctca
     1141 aaggattgat tatgcatgtc gtgatgtgga tattattttt catgctgccg caatgaaaca
     1201 cgttccagca agtgaatata atcctatgga agctgtaaaa acaaatgtga tgggaactca
     1261 aaatgtaata gatgcagcga tcaaaaataa tgtaaaacat tttgtaggta tcagtacaga
     1321 taaagtagtg agtcctacta acaccatggg tgcgacaaag ttattgagtg aaaaattaat
     1381 aatagctgcc aatcagtata aaggaaaaac agaaacaata ttttcttgtg taaggtttgg
     1441 aaatgtcatg gggtcaagtg gatctgttat tcctcttttt agtaaacaaa taaaaaacaa
     1501 taaagatatt actttgacag atgttgatat gacacgattt atgatgtcaa taccggatgc
     1561 tgcaagatta gttctgtctg ctgctatgaa ttcaaaattt ggagaaacat atgtcttaaa
     1621 aatgccggca atcaatattt taactttagc tcaatcatta ataaaaatat ataatgatac
     1681 acatgatgaa aaatatagag gtgaaataaa agttgtaggt atcaggccag gagaaaaatt
     1741 atatgaagag ctaatgacta cagacgaaat tgaacgctca tatgaaaatg ataaaatgta
     1801 tatcattcca ccacactatc atgctactga cgaactgtac aaagattatg aaaaaattaa
     1861 aatcactgca tactcttcag atgcaacaga attaatgagt ggacaggaat ttattagaat
     1921 cgcaaaagag aataatgtcg tgacttggta aggagtttta tgatgaaatc agaagtatat
     1981 gtaattgcag aaattggtgt aaaccataat ggaagtctag atttagcttt agaaagtatt
     2041 gataaagcta aagaatgtgg agctaacgcg gttaaatttc aaacatttaa aacgaataag
     2101 ctagtttcca aaaaggctcc aatggcaaaa tatcagaagg ataatcttaa agaggacagt
     2161 tctcaatttg aaatgctaaa aaaattagaa ttatctcaat ctgattttat tagaataaaa
     2221 gattattgta tacaaaataa aattgatttt ttgtctactc cttttgatga agaaagtgca
     2281 gactttctaa aacaaattgg tgtacaagga tttaaaattg gctcaggaga tttaacaaat
     2341 ttaccttttc ttagaaaaat agatatgtat gggttgccag ttttattatc tacaggtatg
     2401 gctgatattc atgaggtcag tgaggctgta gattgtttca tagatagtcc agttactatt
     2461 ttacattgta cttcaaatta tccggctagt tctgaagata ttaatttatt ggcgattaac
     2521 acattaaaag aaatgttcgg gatacccgtt gggtactccg atcattcatt gggatatgat
     2581 gttggtattt gtgctgttgc gatgggtgct aaggtaattg agaaacattt taccttggat
     2641 aaggaactac ctggtccaga tcataaagct tctttagatc ctcaggaatt tgcagatttt
     2701 gtaaagcata ttagaaacac tgaaatattt ttaggtgatg gtgttaagcg agcaatgcct
     2761 tctgaactta gtacaaaaga agtatcaaga aaaagcctag tggtcactga gaatttaaat
     2821 gtgggagata ttcttacaga gaaatctctt acaataaaac gtcctggaaa tggaatagct
     2881 cctaagtttt tatatcaata tattggcaaa aaagttaaaa gaccaataag tgcagatagt
     2941 gttctgcttg aggaagatgt tgaatgaaaa aaatacttat agtaagtgga acacgagctg
     3001 attatggaat atataaacct gttgctgaga atttactaca agataattgg gatgtatcat
     3061 ttgttgtttc tggaatgcat ttatcccata aatatggatt tactaaagaa ttgatcttag
     3121 ctgacggatt tagaattagt ggagaagttc aaagtttgtt tttagagaac aataagggaa
     3181 acatggctcg gtctgtggca ttagagataa atggtttcac aaatatattt gaacaaacta
     3241 gccctgattt tgtattatta ctaggggata gaggagaaat gttggcagct gcgatagctt
     3301 gtttgtatct tggaatcaaa acagcacata ttcacggagg cgaagtgtct ggtactgtag
     3361 atgaatcaat aagacatgcc ataacgaaaa tagcatctgt tcattttgct gctacggaaa
     3421 aaagcaaaga gcgtttaata aaaatggggg aagattcttg gagagttttt cccgtcggag
     3481 ctcctagaat agatactatt ttaaataata gattacctag ttttagagat ataaaagaaa
     3541 aatatcatct aaaatttgaa aagaaaaaat acgttttgca agtatttcat ccagttgtaa
     3601 ccgaactaga aaatatagaa aaacaaattt tagctttgat caattcaatg gaacaacaat
     3661 ctcaaccaat tatttgcatt ttacctaata gtgatgctgg tagcgaaatt atcagaaaaa
     3721 tgtaccagaa atatttaaaa acggatatta tttatattac gaatttagag ccggaggaat
     3781 acctgactat attaaaagag tgcaaattta tgatcggaaa ttcttcttct gggataattg
     3841 aagctgcatc attcaaaata ccagtaataa atttaggaac cagacaaaat ggacgagaac
     3901 aatctaaaaa tattataaat gcaaatctaa atgaaaatga aattgaaact gctttgaata
     3961 aaatacaaga tcctcagttt ataaaaagct tgaaaaatgt taagaacatc tacggtgacg
     4021 gcaacagttc tagaagaata acaaaaacat tgaatcaaat attgaatgat ggggaagata
     4081 ttaagtggat tcaaaaacaa atagcttatt aaaatatact agtgatccat acattattat
     4141 tggggctggc ggacacgcaa aagtcattat tgaaacctta agattagaaa ataagttaat
     4201 atatggattt tttgatgata agcaaaataa aatgcaagat ctgaaatatc ttgggaaaat
     4261 aaacgaattg aatagctggg cggatagcaa aaaaaaaatc tataaattta ttgtagctat
     4321 tggtgactgt gtagtaagaa aaaagattat aaaaaaattg aatatgccaa gtgagcaata
     4381 tggaatagct gttcatccaa gtgctatagt ggcaaaagaa atttcaattg gttatggaac
     4441 tgttattttt gcgaatgctg taattaaccc agatgctgta attggagagc atgctataat
     4501 aaacacaggt agcattgttg agcatgattg tagaattaat aactatgttc atttatctcc
     4561 aggtgtatgc ttagcgggtg gagtacatgt aggtgtagga actcaaatag gaattggttc
     4621 tcaatgtatc cagatgaaga aaattggatc ttggtgtatg ataggcgcag ggagcactat
     4681 tgttaaggat attccctcac attccttagc ttatggcaat ccagctaaaa ttaagaaaga
     4741 aggaattaat tttgaattct agaattttat tatcaagtcc tcatatggga ggaacagaag
     4801 aaaaatatgt tgctaaggct tttaaagata attggatagc gccattaggt gaaaatgtgg
     4861 ataaatttga agaaagtatt acaaaatata ctggttctaa gggcggagct tctgcgttaa
     4921 gttcaggtac agctgccata catcttgcct taagactgct taatgttgga aaaaacgata
     4981 ttgtctttgc tccttcattt acttttatag ctacagtaaa tcctatttta tatcaagggg
     5041 ctgtaccagt gttcattgac tcagaaccta atacatggaa tatgagccct gttgcactag
     5101 agagcgcatt agaagaagct gaaagaaaca accaattacc taaggcaatc atcgtagtga
     5161 atttatatgg tcaaatggct gattatgaac gaattttagc tattgcaaac caatttaatg
     5221 ttccagtgat agaagatgca gcggaatcgt taggttctac ttataaggga atttcgtcag
     5281 gcttatttgg gacaattggg atctattcat ttaacggtaa taagatcatc acaacctcag
     5341 gtggaggagc tctagtgtct agtgataaaa aatatgttga gcattcaaga tttttagcaa
     5401 cacaagcaag agatcaagca ccgtattatc aacatagcga aataggttat aattaccgtc
     5461 taagcaatat ttctgccggt atcggacgtg gacaaatgga agtattgaat gatagagtag
     5521 ctaaacgaag agaaattttc aatcgatata aggaagcttt ttctggaatt gatagaattg
     5581 aaatgatgcc agaagtggat ggcatggtgc caaatcgttg gttgtctact ttaacgatca
     5641 atgattataa ttctattgat caagtagtta acgtaataaa taaactaaat gaaaaaaaca
     5701 ttgaaacaag agctttgtgg aaaccactac atttgcaacc agtatttcaa ggggtaaaaa
     5761 tgtattacca tgataataat cttagtgtgt cagaaaagtt atttaatagc ggcttatgct
     5821 taccttctgg aagtaatatg acagatagtg aacaagaatt tgttattgat gagtttaaaa
     5881 aaatattcaa gtaggtgtga tattttatga acataggaat tattggttta ggttccatgg
     5941 ggaaaagaag acttagactc attcaagaac actttaatga tattttacta tgtggtgttg
     6001 atactaatga ggcgagacga aaggaagttg aaaaggaatt tggaatacca acttataata
     6061 gtctaacaga ggcaaaaaat ttgtttgaaa taaattcagt ttttgtttgt acatccccta
     6121 taacccatga atttattata aaagaggctt taaatttaga ttgtaatatc tttacggaaa
     6181 tcaatttatt aaatgaatat tatgatgaaa taatcgaagt agctaaaaag aaagatagat
     6241 tactttatct atcttctaca tttttgaaga gaaaagaaat tcaatatatt aagtctaaag
     6301 tgacgcataa taaaaaaatt tcttatcaat accatgtagg acagtattta cccgactggc
     6361 atccttggga agattatcgc aatttttttg tttcaaacaa aaaaactaat gggtgtagag
     6421 aaatattcgc tattgaatta ccttggatta tagatacatt tgggaaagtt aaatcgttta
     6481 ctgtaatgac ccagaaatta tccaatcttg atattgatta ttcggacagt ttcactctta
     6541 ttattgagca tgaaactggt atagttggaa ctttaagtgt agatgttgtt tcaagagtag
     6601 ctaaaagaaa tttaactata attggtgaaa agattttaat agaatgggaa ggtaacccgg
     6661 attctctatt ttcatggaat gaagaaactt ctagtatgac agatattcaa ttgtacgatg
     6721 aggttatgca tgataataat tattcacata ctattattga agatgcatat ttagaggaga
     6781 ttaaagaatt tttcacgctt ttggaaaaga aaatggaacc ccaatatact tttgaaaaag
     6841 ataagtatat tattaactta attaacgaca ttgaggtaaa ttaatgatga aagtaatttt
     6901 ttttgggtta gggtcaattg gaaagagaca tctaaaaaat ctaataagct atggaaaaaa
     6961 gaattctatt aattttgata taacagctta tagtagcagt ggaaaaaaag agtttgaaga
     7021 aataacgtat attcaaaata gagacgattt ggatacacat tatgatatag cttttattac
     7081 aaatccgact tttaagcatc tggaaacact aaatgtaatt gttgaaaaag ttgattacat
     7141 atttttagaa aaacctgctt tttctgagag tattgatctt actcaatata attatgatta
     7201 caaaaatatt tatgttgctg ctccactgag gtataaaaat atcatggaag ttgttcgtaa
     7261 tagtattcaa agtaaaaaag tatattctgt aagagctatt tgttctactt atcttcctga
     7321 ttggagaaaa ggtactgatt atagaaataa ttattctgcc tttaaatcta tggggggagg
     7381 agtagaatta gactgtatac atgaattgga ttatattata aacctatttg gtttccctaa
     7441 atcagtaaaa aaacagtttg gtaaatattc
//
LOCUS       DAZKNH010000111         7218 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000111 DAZKNH010000000
VERSION     DAZKNH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.111"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(230..907)
                     /gene="phoU"
                     /locus_tag="ACG8MG_002355"
     CDS             complement(230..907)
                     /gene="phoU"
                     /locus_tag="ACG8MG_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFQ0376361.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(920..1678)
                     /gene="pstB"
                     /locus_tag="ACG8MG_002356"
     CDS             complement(920..1678)
                     /gene="pstB"
                     /locus_tag="ACG8MG_002356"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0376362.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1694..2500)
                     /gene="pstB"
                     /locus_tag="ACG8MG_002357"
     CDS             complement(1694..2500)
                     /gene="pstB"
                     /locus_tag="ACG8MG_002357"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0376363.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2515..3399)
                     /gene="pstA"
                     /locus_tag="ACG8MG_002358"
     CDS             complement(2515..3399)
                     /gene="pstA"
                     /locus_tag="ACG8MG_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFQ0376364.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3399..4319)
                     /gene="pstC"
                     /locus_tag="ACG8MG_002359"
     CDS             complement(3399..4319)
                     /gene="pstC"
                     /locus_tag="ACG8MG_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFQ0376365.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4434..5291)
                     /locus_tag="ACG8MG_002360"
     CDS             complement(4434..5291)
                     /locus_tag="ACG8MG_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFQ0376366.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5628..7076)
                     /gene="cls"
                     /locus_tag="ACG8MG_002361"
     CDS             complement(5628..7076)
                     /gene="cls"
                     /locus_tag="ACG8MG_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0376367.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2093 a         1589 c         1255 g         2281 t
ORIGIN      
        1 tttgcattct ttatcctttc tatgaaatga tgaaaagggt ttgtgacatg ttttgtcaca
       61 aaccctttcc cgaataaacg gtgttcaaaa gcttgcatct gcggtaataa gtccagccaa
      121 acgaaaaata tgaggagcta ttttttgttt gactgttctt attacttgat gtagaacagc
      181 tttttcacaa cctcttttca taaacaaacg gcataatcac agcttcagtt tagaaaatct
      241 cattgtggtt cgtatttaac tcagtgattt tccctgtagc caagtagacg atccattcac
      301 agatgtttgt cacatagtct ccgatccgtt ctagataact ggcaacattc aagtagtctg
      361 ttccactgat gactgtctca ggattttcct gcatattttt gattgttgca ttatagattc
      421 gattgaaata gtcattgact ctttgatcca tattggcgat cgtacgtgcg tctttttcgt
      481 ctgttttgac ataagcaact aggacgttgt ccaccatttt tttcacataa tcggacatat
      541 cggagatctc tttttcgatt tccggtattc tcgtctgccc ttttacgcga atcgtggatt
      601 tagcaacaga caccgcatga tcggccatac gttctaaatc cgagctagct ttcatgaccg
      661 taatgatcat acgcaaatca gttgtaactg gctgttgcaa tgcgatcatt tcaaaacttt
      721 tcttctccaa gcgtgtttcc atgttattga tatttatatc gttttcgata acttcttggg
      781 ctaagatttt atcgtgtttg atgtatgcac gaactgactt gtgcacggcg ctgctcacca
      841 tcatccccat ttcgtagaac tgattatgga gattcagcaa ttcttcttca aattgtgtac
      901 gtagcattcc gtttcctcct tatccaaatc gacctgaaat ataatcttct gtctcttgtt
      961 tttctggatt catgaatatc ttcttcgtat cgttgtattc gatcaagtga ccatctaaaa
     1021 agaatgccgt cttatctgaa atacgtgaag cttgagacat attatgggtc acgatgatca
     1081 tcgtataccg ctctcttaat tctaacagca tactttcgat tttcccagta gaaacaggat
     1141 caagcgcact tgttggttca tccaataaga tgatttccgg attaactgcc aaaacacgtg
     1201 cgatacatac acgctgctgc tgcccgccgg ataaagataa agcactttta tgcaatttgt
     1261 ctttcacatc atcccaaaca gacgctgcct tcaggctttc ttcaactact tgatcaagga
     1321 cttttttatc tttttcccct tttaatttca atccatagat gacattttca tagatagaaa
     1381 aaggaaatgg gtttggctgc tggaatacca tgccgatttc ttttcttagt tcaacaacat
     1441 ctgtttttgg accatagatg tctttgtctt tatataaaac acttccggtg atcgttacat
     1501 tcggaatcag gtcattcatc cggttcaaag aacgcagata agtggatttt ccgcaaccag
     1561 aaggaccgat catcgcagtg atttcccctt gattgaaact tagatcgatc ccttttagtg
     1621 cttctttctt tccataatac agatgaagat ctttagatga aataatttct tttgtcatgt
     1681 tgaggcctcc gtcttaacca aaatgaccgg acacataatc ttcggttgcc tgaatttttg
     1741 gtctggtaaa tatcttgtta gttttgtcat attccattac ttttcctaga tagaaaaagg
     1801 cagtatagtc gctgatacga gctgcttgtt gcatgttatg cgtcacgatg atgatcgtat
     1861 agtcttcttt caagtttaca agtgtttctt caaccgttcc tgtcgaaata gggtccaaag
     1921 cacttgccgg ctcgtctagc agcaagatat caggtttcat cgcgatcgca cgagcaatgc
     1981 ataaacgttg ctgttgcccg ccagataaag ccaaggcact tttgtccaag ctgtctttta
     2041 cttggtccca caaggcagct tgtttcaagc ttgtttcaac gatttcatct aatttctttt
     2101 tgtttttttc gccgtgctgt ttcaaagcaa atgtaatatt gtcataaatc gacttgctga
     2161 acggattagg acgttgaaaa accatgccaa tccgtttgcg catctcataa acatccactt
     2221 gaggcgcatt aatatctacg ccttgataca tgattttccc agtgattttc gtattagcga
     2281 tcccatcatt cattcgattc aacgaacgaa gatacgtaga ttttccgcag ccggaaggac
     2341 caatcagtgc agtgatctta tttttttcaa attccagatc cactcctttg attgcttcat
     2401 tgtcgccata ccagacatgt atatcatccg tatatagtgc tacttctttc tcttttggca
     2461 tggtgatgac atttgtttca ttccagttat attttttcat tttgcttctt ctccttaggc
     2521 tgaagtcatt tttcggtata atcgtttgcc aatcgcacga gcgccgaagt tgaacagtaa
     2581 aacagcaatg atcaacacgg cagaagcgcc agccgaaaca gaaacaccat caggcatcgt
     2641 gccttctgta ttgatcttcc aaatatggac agctaatgtt tcagcttgac ggaaaatgct
     2701 gatcgggctt gaaacactta atggattcca gttcgtaaaa tctaacgcag gtgcactttg
     2761 tccagccgta tagatcaatg cagctgcttc accaaaaatt cttccagaac ttaggatcac
     2821 accagtcaaa atccctggtg tcgcttctgg aaccaccact ttcatcactg tttcccagcg
     2881 tgacaaacct aaggccaatc ctgcttctcg ttgtgtgtaa tggaccgctt gtaaagactc
     2941 ctcaactgtt ctagtcaaaa gaggcaagtt gaatacagtc aaagccaatg caccagaaat
     3001 aatcgagaaa ccatactgga attgaataac gaaaaccaag aaaccgaata aaccgacaac
     3061 gacagaaggc aacgagctca agatttcaat cgacgtgcga atcacatctg ttagccagtt
     3121 ttttccagca tattctgaaa gatagatacc cgcacctaaa gaaataggaa aactgatgat
     3181 catcgtgatc aaaagcaaat aaatcgagtt aaatagctgg atcccgattc caccaccttc
     3241 ttggtaggct ttggaaggtt cggtcaagaa ggaccatgaa atgtgcggta ttccccgaac
     3301 taagatataa agtagtaaag ctgctaaaat caatacgatg atccccgaaa ctatatataa
     3361 aacacctgta gcaattttat ctgagcgttt tgcgttcatt atttcagctc tcctttcttc
     3421 ccaatcatac ggatcacgat attaaagaat aatgacatca atagcaagat cagcgctaat
     3481 gaccataaca cattattttc aactgttccc atgattgtat ttccgattcc cattgttaag
     3541 atggacgtca atgtagaagc aggagtagta agtcctgttg gcatcaatgc tgcattaccg
     3601 atgaccattt ggatagctaa ggcttcacca aacgcacgag ccataccaaa aacgactgct
     3661 gttaaaatcc cgggcacggc tgctcgtagc acgactttgt agattgtttg ccatcttgtg
     3721 gcaccaagcg ctaatgaagc ttcccgatag tgccgtggta ctgcctttaa tgcatcaaca
     3781 gtcatcgttg tgacagtcgg caagatcata acgaacaaca caaatgtacc agctaagata
     3841 ccaaaccctg tcccgccaaa aatacttctg acaaaaggca cgataactga taaaccgata
     3901 aaaccataaa cgacagaagg aatacctaca agcagctcga tgaccggctg taagatcttc
     3961 gttccgtttt taggagagat ctccgtcatg aatactgctg caccaatggc aaatggcgtg
     4021 gcaacgatag cagataaaaa ggtcacaata aacgaacctg tgatcatagg caatgcccct
     4081 accattggtt tcccatttgc tccgatttgg cttggattcc aatctgtacc aaacagaaaa
     4141 tcaaatacat tgatcttatc agtaaaaaag gtggccaatc ctttactagc tacaaaataa
     4201 aaaatagata atacaaccaa cacgatcagc gcgatacata aaaaactgat gaattttcca
     4261 cgctgttcca tttttgaacg ttttgatttt gttaataata cttttttcac atcttccaca
     4321 gatgactccc ccttaatcag gtttaacata ctcactcagt gtacaaattg aacatcaatt
     4381 tggttttaat aacatgtaaa agtttagcta agtttgtaaa ttttgtgtaa agattactct
     4441 ttaacgatat ttccttgcca atcacgttcg actttcatat gagaaacagg gatataacct
     4501 agttctccaa cgatgttatc ttgtacttca tcagataaga tatagtctaa aaattctttt
     4561 gtcagttttt ttggatgatc tcttgtgtac atatgctcat atgcccaaat cgtccatttg
     4621 ttcgttgtca cattttcatc tgtcggttct acaccgttaa cattcaacgt atctacatca
     4681 tcggaaacat aagaaaaagc aacatagctg attgcaccgg gtgtgtcggc aacgatctgg
     4741 cgcaccattc cgcttgaatc ttgttcttgt gcctgaacag ctgtttctcc gtccaatacc
     4801 catttttcaa acgtgctacg ggttccgctt cctgaagcgc ggttcaatat aactactggc
     4861 tgatcttttc caccgacttc actccagttt ttgatctcgc ctgtaaatag ttttttcagg
     4921 ttttccatac tgatgtcttt tacgccaata tccttgttca caataggagt gatcccaaca
     4981 actgctactc gatgatcgac taatgctgag gcatcgatcc ccgatttttc ttccgcaaac
     5041 aaatcagagt ttccgatgtc gactgctcct gactgtactt gagacaaacc agttccgctt
     5101 ccgccccctt gtacattgat gaattttcca ggattttctg tctggtaagt ttcagcaact
     5161 gtttctacta aaggctgtaa agcagaagag ccaacagccg taatggattc tcctcgatca
     5221 atccactttg aacagcctga taaaagaatg gctaatccta ttattggtaa gaataattta
     5281 agttttttca ttagaaatct cctttaatcc taaagtatgt ttattttatc attttcatta
     5341 cttgatgaaa acgaatcaaa atcattaaat aacaaaataa attctcataa ttgtaaaaaa
     5401 aactatctca ttaaaagttt ctatgctata ctggctttga aatgtgtctc ttttaatagc
     5461 atatattagc ttatttcttg ctttccccaa aaaacaagag ataccccaaa gttaatttcg
     5521 ctcatgcgag acgctttctc catttctgac gtactctcag aaatggcccc gttagaaaag
     5581 gacacgagat ctccatctgt tgatggttct catgtccttt ttatgtttta aaggagcggc
     5641 gaaatcaatc ttgctaatcc ttctttcatc ttgatgatta ttcctcggtt ttcatacatt
     5701 tcttttgtga gatgcgtaga aattttcgag tcttcaaaaa aagcttttct tacacgtgaa
     5761 gccattcgtt catcataaac cagtgtattg acctcaaagt ccaagcgaaa tgaacgaaca
     5821 tcgatatttg ctgagccaac tgaaacaatg ccaccatcga taatcatcgt ctttgcatga
     5881 ataaatccat tctcatatgt ttcgatcctt gctccatact cgatcagctc tgcagcgaaa
     5941 gaatacgttg cccagtaaac gagaagatga tctggtttgt taggtatttg gatatgaact
     6001 tttacgcctg aaagaagagc caacttcagt gcttcatgaa tcgatccatc cggtatgtaa
     6061 taaggcgttt ggatcaatat ctcccgtttg gctaacgaaa tcattttcaa ataagtcatt
     6121 ttgatttgtt catgttcggt atctggtccg cttgtcacga tttgaacagc tatatcacca
     6181 gtagtgtgaa tggacgggaa aaatttggga gaatagccta cttcttttgt atgctgagaa
     6241 ttccagtcca tcaaaaaacg gttttgcaag gtatacaccg cttctccgta aatacgcaaa
     6301 tgattgtctc gccaataacc aaatttcttt accaatccta gatattcatt tccaacattg
     6361 aacccacctg tatatccgat tttcccatca ataactacga ttttacgatg attgcgataa
     6421 ttgatccgag gattaatata cggaacaaat agaggaaaga aaaatgcgac ttctccgcct
     6481 gcttttttca aattctgaaa aaagctggaa gaaacttgtg tggaacccca tgcatcaaga
     6541 aggactctga cttttacccc tcgtcttgcc gcctctgtca atgcatctcg aacttcttca
     6601 cctaatgcat cactgcgata aatataatac tggaagtgga tatggtcttc tgcctgcttg
     6661 atatcttcaa tcagcgcatc gaatttttca cgaccatctg taaataaagt gatttcattt
     6721 ttagtcgtat agagcgtact ttcaaatacc gtgagcatat agatcaattg tttcacatct
     6781 acttgtccag taggcggatg aggataaagc cctcgcatca atgcctgttt gtcttcttca
     6841 atttccaggt tcttaccgat tttcccttgg atcttcaaat caaagatttt ctctttggaa
     6901 atccctcttc ctaaaaaaat atataaaata aatcctacaa ctggaatgaa catcaaaacg
     6961 agcagccatg cccatgtttg agcagtttcc tttctttctc gaaaaacgat gataaaggaa
     7021 agcaaaatat tcataatcaa aaggatcgta aaaaaattgt caacgataat tttcacggct
     7081 tcacctcttc ttctaccaac ttaagaatac ccaagttgtt ttttaaagta aagggaaaaa
     7141 agccgcttcc ttctattctc ctagccaata aaaaaagaaa gctggaacga aactgtccaa
     7201 ctttctcatt ttatttat
//
LOCUS       DAZKNH010000112         7011 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000112 DAZKNH010000000
VERSION     DAZKNH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.112"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            323..919
                     /locus_tag="ACG8MG_002362"
     CDS             323..919
                     /locus_tag="ACG8MG_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0376368.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(977..1459)
                     /locus_tag="ACG8MG_002363"
     CDS             complement(977..1459)
                     /locus_tag="ACG8MG_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376369.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1565..1762)
                     /locus_tag="ACG8MG_002364"
     CDS             complement(1565..1762)
                     /locus_tag="ACG8MG_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376370.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2057..2815
                     /locus_tag="ACG8MG_002365"
     CDS             2057..2815
                     /locus_tag="ACG8MG_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0376371.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2875..3303
                     /locus_tag="ACG8MG_002366"
     CDS             2875..3303
                     /locus_tag="ACG8MG_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376372.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3349..3421
                     /locus_tag="ACG8MG_002367"
     tRNA            3349..3421
                     /locus_tag="ACG8MG_002367"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3382..3384,aa:Gln,seq:ctg)
     gene            complement(3571..3942)
                     /locus_tag="ACG8MG_002368"
     CDS             complement(3571..3942)
                     /locus_tag="ACG8MG_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0376373.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            4157..4342
                     /locus_tag="ACG8MG_002369"
     CDS             4157..4342
                     /locus_tag="ACG8MG_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376374.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(4425..4613)
                     /locus_tag="ACG8MG_002370"
     CDS             complement(4425..4613)
                     /locus_tag="ACG8MG_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376375.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(4637..4900)
                     /locus_tag="ACG8MG_002371"
     CDS             complement(4637..4900)
                     /locus_tag="ACG8MG_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376376.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            5058..5315
                     /locus_tag="ACG8MG_002372"
     CDS             5058..5315
                     /locus_tag="ACG8MG_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFQ0376377.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(5386..5577)
                     /locus_tag="ACG8MG_002373"
     CDS             complement(5386..5577)
                     /locus_tag="ACG8MG_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376378.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(5718..6038)
                     /locus_tag="ACG8MG_002374"
     CDS             complement(5718..6038)
                     /locus_tag="ACG8MG_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376379.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(6061..6495)
                     /locus_tag="ACG8MG_002375"
     CDS             complement(6061..6495)
                     /locus_tag="ACG8MG_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0376380.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            6830..>7011
                     /locus_tag="ACG8MG_002376"
     CDS             6830..>7011
                     /locus_tag="ACG8MG_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0376381.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2204 a         1363 c         1070 g         2374 t
ORIGIN      
        1 aaaaaactta tttaagaaat ataaactaat tttctataaa agcaagtaat ttttaatggc
       61 aatatcataa aaaaaagaag atagagattt aataattata tatcagctgt ttttttcaaa
      121 taagcaaaaa ttcctaaaac acaacaatga aagcgataac taagaaaatc cttacataac
      181 gatttttctt attaaaattg ttttttgaac ttcgcttatt ttttgtgagc atatctgttt
      241 acttactttt tgtaaggtgc tatacttcat ttcgtaatca attaaaaaaa tgcaaaacgc
      301 aaatcaaagg agaaatttta ttatgacaac ttttactgac actttgaaaa accgccgttc
      361 aatctatcat ctaggacgta atgtttcact ttctaatgaa gaattaacaa ctttgatcaa
      421 agaagcaatt aaagaaagcc caactgcttt caatgctcaa tcaacacgcg cagttatcct
      481 tttcggagat gcacatgaaa aactttggga aatcactgaa gaagcacttc gcccattgac
      541 tccagcagaa gctttcccaa atacacaaaa caaattagct ggtttcaaaa atggttatgg
      601 tacagtatta ttcttcaaag atactgacgt ggtaaaaggt ctacaagaac aatttgaatt
      661 atatgcagac aacttcccag attggtcaga acaatcaaac ggaatcgcaa cagctaatac
      721 ttgggtagca ttagttgata aaggattggg cgcaaacttg caacattaca acccagttat
      781 tgacgaagca gttgcaaaag aatggaatat cccttcaaac tggaaactac gttcgcaatt
      841 agtattcggt tcaccagaaa caccggctga ggaaaaagaa tacatgaacg atgccgatcg
      901 cttccgcgta tttggataat tttatttgaa ataaaaaaca gaggttgtga caaacatctt
      961 gtcgcaacct ctgtttttat tctggatcaa tcataatatc aatctctgcc tcatcgttta
     1021 agcgttggga caaaaatgga actaattctt ctaatgatgc agaaaatttc agcatcggtc
     1081 ctcgccatcc gtttcggaaa aaattcattt ttacgatttt agaacctttt ggctgatata
     1141 atacaatttt attgatctca gaaaaaggaa cacctttttt gtcaaagcta ttcaaaacaa
     1201 gtccttcttc tgtgattcct ctactgtcca acaaaaaact aataaaaatc attgctgaca
     1261 agatcccttt tgccaattca tctgctgtag gatgactgat gagtgtatac caaatgacta
     1321 gtgacactaa gaagggtatc accaagtaga cccatcgatt tcgcacagtc cgataaataa
     1381 tttcttttcg aaccagccaa agaaataaaa acatcacaac aacgaataaa aacaatagaa
     1441 ccgtaaagaa tgttaccatg acaactcgcc tcgctttttc ttctccagaa aataggaaaa
     1501 gaatactgat ttaccttctc aaagaaattg gacttgcagc acgccgcacg tccaattgtt
     1561 ttctttattc aaatgtatca tattttgcaa caaccatgct tgctttttca tctaataatt
     1621 cttggtcctc tgcagcacat tgggcaactt tgaccacggc gcagtttgtc atttttgata
     1681 caacttcacc aatgaccgtt tttgtaaaac caattggttt gcatgcatat tgttctccta
     1741 aattaatcat ttcttccgtc atcaaaatcc tcctaaactg ctggaacttt tctccacatt
     1801 accactgcta tttctttgga gatttaactc attgattttt atttacagtt aaaaatcaac
     1861 cttacaggca ttatagcgta ctgttttcgt tttcacaatt aaaacagctg ttgtatttag
     1921 aagaattttt ttcaaaaaat taaaacatat tccttaaaaa ctaattattt cctacatatt
     1981 aaaatataat acttccattt ccaataaaaa tacatttata ataggaaaga taaacagctg
     2041 aaaagggaga tttcaaatga agaaaaaaac gttcaaaaaa ctattgtttc tagggggcac
     2101 agctgctctt ttattaagcg gtacaagtta ttacctacat atccattctc cagaacatac
     2161 aatgaacact gcacaaaaaa gttcaacaca aaaaacgaaa aagacaaaag agaaagcaga
     2221 agttttacta gatgttccat tagagagcca gtttgattct ccttctttag aaaatggctg
     2281 tgaagtcact tcgctttcta tgcttttatc tttttatggt tatgaaacaa ctaaaaacca
     2341 gttagctgag caattggatt acgtccctgt tttcaatgca gatgggactc atggcgatcc
     2401 taatgaagga tttgttggtg atatcagtgg cggagactgg gctatggtcg tctatgttcc
     2461 cccagttgcc tcccttgctc agcaaatcgt ccaaacggat tatcacactt ttccaaaaac
     2521 cgatgctcaa ctcgatgata tcaaaaaagc actatctaaa ggaaatcctg tctggacttc
     2581 tgtgactatc aattttgaag tcccagatga aactgatttt atgacttgga ccacaaataa
     2641 tggagaagta agagtcactc ctcttgttca tgcttgtgta gtcactggat atgacaaaga
     2701 aaatatttat gtcaacgatc cctatggtgt aaaaaatcga gcagtaccga tcaacgattt
     2761 ttcggcgatt ttcactgcaa tgggtggaca gatgctaact ttggagaaaa gctagtaatt
     2821 caaacaaaaa tcccttatac tcaagatacc aaacctttga agggagcgta gataatggaa
     2881 aaattcacac ataaaaaaat ggatccaaat gaaattccga ttatttttgt ccgtgactgc
     2941 aagggaaatg ttcaaggtaa agtctctatt aatgagtgga acgagcgtcg cagacctgct
     3001 accctcaatg aactggaaat caaattgtat cgtcaatctc ttgtttatta cgcagatcaa
     3061 gagtacgaga aagccaccga tttattgaaa tttctaattg ctcgaacaga gtatactcgc
     3121 tttgaatata ttgaacgttt agcaaacatc tatcatatta tgaatgaacc tgtcaaagaa
     3181 tatcagttat tggacaccgt cttgtcagta gcagaactga ttgcccttcc tgctggatta
     3241 gaaaagaaac tagtacgccg ccttttacga gtaaagcaac aactatccga tcaagaaaaa
     3301 taacttgcgt ttttttagga aagtgctatg attattttgt tgaataaatg ggatgtagcc
     3361 aaactggtaa ggcacttgac tctgaatcaa gcaattgtag gttcgaaccc tgccatccca
     3421 atttgatgtg cttgctgaaa aaatgaaccc cctgtaaaag aacaatcttt ttgcaggggg
     3481 attattgtat tatttttact acttgtattt agtttacata attaaattat gaataataaa
     3541 atcgggcgta ttcttaatac aaaaactgat ttacccaata attgctaatg ctttctccgg
     3601 aactttttct ctaggaactt cctcatacgt ttctatgtgt gctcctttat tgctaatttt
     3661 caaataatga ttttctttta acacagaaag accattaaat tcaaccgttc gttctttccc
     3721 attggcatca gcagctttct gcacatacct tgctgtacca tattcattta cttcctctgg
     3781 tttttgtgtc tttacataga cctctccttt ttcaactaat ggatttagct gatcaagtat
     3841 accagggatt tctccataac ttccttcagt ataatatttc agtccaaata gcaaaatacc
     3901 actaatcgcc gccagtccaa tcaaatattt aatcactttc atctttctac ctccttgtta
     3961 tatattcagt atatgggtca gttaaagaaa ttccatccgt cccaagttgg agatttttct
     4021 cctactttag aagcgagaat cgagtgtttt tttagtaaaa atgatcgtta tttgatatta
     4081 ttgacgtgtg gataaatcag tttttctcgt tttgtccatg taaaaaatca tgttgatagc
     4141 aaaggatgtg ttcaaaatga atcaagaaga attatttcaa aaagtcaagg aaatgattaa
     4201 gaacggcaat ttcgatggcg caaaacgatt tatcgaagaa cataaagaac aattaggacc
     4261 ttataaagag aaagcccaaa atcttttaaa agatgtaaat atcgacagtg tcaaaaacaa
     4321 atttaaaaat ctattcaaat aagtaaaact ttcacttttg taaaatcaaa ggaggacaat
     4381 gctgctgcat cgtcctcctt tggttctttt ctgaatactt catactaaaa taaacgtgtc
     4441 ggatcgtaat gataagtcac attgccgttc agtgaggctt ttcccaccac atatttacga
     4501 ttctccaact tcagccacgc ttctcgaaaa gccattaatt cttctctttt tacatcaatc
     4561 ttttcgattt ttccatcgat caaatcttga ataagttgtt catatggatt catttttttc
     4621 ctccatctgc tgtcaatcaa taagttgtaa aaataggttc atatgctttt ttggtatcta
     4681 gatcttctct gactttgact gtcgtataaa taattttttc tactgggata ctataataac
     4741 ttccttcacc attattgaat cggtgaacgt ctttctttgc taaaatacca tttattttat
     4801 tcgtcagtac acgttgttcg acactgttac ttacaacata gtaaatcacc cgaatattat
     4861 tgatcaaatg caaatcgatt tctaacacta catgatccat gaaaatccca ctccttcttt
     4921 cactttgatt atagcaagtt tctcctctaa tttcaaaatc gatacagatt ttaccggata
     4981 aatccacaaa actgcttgcg ccatttcgca caaagtgtta ttttgtatca tggactttat
     5041 ttatcggagg caatagtatg atcgcgatcg gtcaatttgt tttttatatt ccttttttta
     5101 ttatgataag cattcttttc tattacataa aatggacaaa aaagaagttt tcagttttac
     5161 tggcctcttt acctgctgta tattttactt atcaaatttt ttcttttagg cattgggaaa
     5221 caacgtctgt attaataaca cacatcatcg aattgacgct ttcagtaatt tttcttatta
     5281 tttggatcta ttttttatat aagaatcaga attgaatccc tcgcatgttt aaaaagaaaa
     5341 cgattaaaga tgcagcaaat ttctgccgat ctttaatcgt tttttctatt ctgttatacc
     5401 aaaaaaatgt ttgaatcggt gaaggatcgt tccttgtttt aattctataa attcttgtgc
     5461 gatcgggtac ttttcttcca aattttcttt gttcttttcg atcaggcttt tcgcttcttc
     5521 atatttttta tctgcacata gtgcttttac ttcctgtaat acttttcctg cttccatttg
     5581 cctcaccctc ttgccatttt attgctttaa gttttattta agttatacgc attattctag
     5641 tttcatccca aataaccttt tttcattttt actcctcgaa ctaatcagtt ttctaaaggc
     5701 tgattggttc gtctttttta tttttcctcc atttcattct gctcttctgt taatttatct
     5761 aatgctttga ttgatgctgc ggctcttgca taatcatagc tgtataattc cataaattta
     5821 tcaggcagaa caaaccacgc tggatgataa ccgttagctg tgacagggaa tcctttatct
     5881 tgttggttat ctccatccca tctcagagca acaccttttt ttcctttcca ctttactaaa
     5941 gcgatcgagt atcccttctt tgtttgtgtg ccgtcttcaa taattgctaa aatttctatt
     6001 ttttcttttg gcgcacggac ttcttgagga cgaaccattt tctattcctc ccttatctca
     6061 ttacttcacg accaacacac tgcaaggggc atgtgatacg acataagaag cggtcgatcc
     6121 aactaatgct tgttgtatag caccttttcc tgtcgctccc ataacgatca aatcaatatt
     6181 ttcttgtcca gggatgacat tggcaatcaa atttcttgga tctccagttt caactattgc
     6241 gtggatctca gcaatgccgt actctttggc atcattgatt tttttcagca tggctacttc
     6301 tgttctttgt ttttcatctg catagatttt tgaaaaggca taggcactat gggtcagctc
     6361 aactttgttg atgattgaca cgataaataa gtgggcttca ttgtcctttg ctaagtcaag
     6421 ggcttcagcg aacgctttga tcgattgtct tgatccgtca actgctacca tgatgttctg
     6481 gtatctttta ctcattggaa ccgcctcctt tttcttttat tgtaacatct caagcaaaaa
     6541 tacaaaaaat atacgcaata tgtaaataaa atgatgaaca aaagcaattt ctttcaagtt
     6601 atttatacat ttcttttctc atcagaacac ctgttctaat cccattattt ctgcattatg
     6661 tgaaattcct catcattttt cacatcgagc gaacatcatt ctgttacaaa aaacgtggat
     6721 ttgtcataaa ttgtcatgct tcttttacct atgaaatatt ttcacgtgtt aaagtattcc
     6781 tcgttgaaca caacaaataa aactttttaa cgaataaagg agatttttca tgacatcact
     6841 taaaacttta ctttttggaa caactttggc tgccggcgct gcattcttca tgggaacgac
     6901 tgctcatgcg gacgaagctt ataccgttca atcaggtgat actctatcaa cgatttctca
     6961 aaaatacgtt ggtgataact ccttgatcaa tgccattgca gaatcaaatt c
//
LOCUS       DAZKNH010000113         6847 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000113 DAZKNH010000000
VERSION     DAZKNH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.113"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            528..722
                     /locus_tag="ACG8MG_002377"
     CDS             528..722
                     /locus_tag="ACG8MG_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376382.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            913..1162
                     /locus_tag="ACG8MG_002378"
                     /pseudo
     CDS             913..1162
                     /locus_tag="ACG8MG_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1406..2236)
                     /locus_tag="ACG8MG_002379"
     CDS             complement(1406..2236)
                     /locus_tag="ACG8MG_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFQ0376383.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            2444..3778
                     /locus_tag="ACG8MG_002380"
     CDS             2444..3778
                     /locus_tag="ACG8MG_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0376384.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3819..4730
                     /locus_tag="ACG8MG_002381"
     CDS             3819..4730
                     /locus_tag="ACG8MG_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0376385.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4792..5580
                     /locus_tag="ACG8MG_002382"
     CDS             4792..5580
                     /locus_tag="ACG8MG_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0376386.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            5577..6193
                     /locus_tag="ACG8MG_002383"
                     /pseudo
     CDS             5577..6193
                     /locus_tag="ACG8MG_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            6195..6713
                     /locus_tag="ACG8MG_002384"
     CDS             6195..6713
                     /locus_tag="ACG8MG_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0376387.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         2058 a         1186 c         1373 g         2230 t
ORIGIN      
        1 gttattttat aataaagata tgatgactaa tttagctcaa ctattccagc gtcagctgaa
       61 gatattgcaa ttcccgattc cttaatcatt tatctggatg agacagaaag tgaagttgca
      121 acagatgtgc atgtagttga ggataaaaat gatagtttaa aaattaatag ttcagatact
      181 ttactaagag taagtcctat cttttcagct gttatttaga tgcaagtagc aactaagtta
      241 ggtagtactc ctgcaatgga taggtatttg tgtgttcgat atataagtgg tcgaagtatc
      301 aaggttatgt atactggatg ggagatagaa gagttaaaaa ttggaggtca ccgccacaaa
      361 tggaatttag atttagaggt accttagatt tggtatagtt attaaacaca agatggcctg
      421 gtttgtaggg aaagtcgaag agtatttttc cacttctgcg cccctttaat ttttctagta
      481 atgccgtgaa tttgtgatta attggaaata tgaagaaagg agagtctatg aaaacaacac
      541 tagttctatt ctataaaaag catccttatt ttacacttct gataaatata ttgttggcta
      601 gtgttatcgg gatttctgtt gaatatctaa taaataaaga tttcataggt tcttgttttt
      661 atacggcttt atttcttgga cttcttgaag cattttccat ttataaaaaa tcaaaaaaat
      721 aaaatagatt gccgtaagag atctttttca tttcattttt ttctgaaaac ccgttgtttc
      781 tgtcagtgaa gcagcgggtt tttagattta ttgtctttag acctaattga gcgcgccgag
      841 cgattgaaat acaaaaccac ttgctatgcg ggtggagcaa taaagggaaa tagtttagcc
      901 cgtacaaagt agatgtgtaa ataccacatt gtattcacac caaagtatag aagaaaaata
      961 atttataatc aatatcgagc caatattcag gagatcatca agagattgtg caaatctaaa
     1021 atataaattt gggtatcgac acttttgggt ggaaggatac tatgtgacaa aagtaggaca
     1081 aaatgaagcg accatcaaaa agtacattca agaacaagaa aaacatgata gtgcgttaga
     1141 taagctgagt gtacgagagt aagacgatcc ttttaagggc taaggagtag tacataaacc
     1201 ttttaagagg tgggcaaaac tggcaaaggc aatatggctt gaacataaaa tatgaaagcc
     1261 agcgtcttta ggcgctggcc ggtgatttga gcttatagct ctagagaaaa ccaccctttt
     1321 gaagggtggt tatgattaat gactttagtc agttttgctc acaaagcgtg tggaacaaaa
     1381 aacgggtgtt tctacgggta gatatttatt tcttgaatgg aggtgtccca tacatttttg
     1441 gtgttactgg ttctaatttt ggtgcagcgc cttcgatctt aggttctttc aaccattgat
     1501 attcgccttc gccatccagt gctttgccac tagcccaaga accttcacta ctttggtctc
     1561 cctctgagaa gttatagaag tcataagcaa tgtcttttcg ttctttgtca cgcgggaaag
     1621 tacttgggac taagattgaa ccttcttttt cttctaattc tttcactgca cattcccata
     1681 gattttgatg atagctatct cttgcaagga gaacagacag catatctttt acgccacgat
     1741 catcgatcat ttcgtaaata cgtgctactt gaagtcttcc ttgtgattcg gcatttaaat
     1801 tagctctaaa gtctgctaac aagttgccac tcgcaatgat ataggcaccg ctccatggat
     1861 tgcccacgct gtcagcagga cgaggtccta agccagctac gatcgcgtgc tgtggattca
     1921 ttccagagat aatggcaccg attgttggat ctttttgcat cgctgcttct tgatcttcaa
     1981 caggcgcacc ctctaataaa cgagcaacca ttgtcgccaa catttccacg tggccgattt
     2041 cttctgttgc aatgtccatc agcatatctt tatatttttc ttctcctcgg cagctccagc
     2101 cttggaacag gtactgcata gctacactca tctcgccata ttggccgccg atcagctcct
     2161 gcagatattt cgcgattaat ggattcggac gatctggttt tgcttcaaac tgcaattctt
     2221 tctggtgtct aaacatcaaa attcctccta atctattttt aatacattct cattctgcta
     2281 cgaaagatta aaaaattcaa agaaagcgct cgcgaattat ataaagtatt ggtatcatta
     2341 ataatttttt tagaatcata ccttataaat tgttattttc agaacaaaat gatatcgtta
     2401 tattaaaacc ggcatcggtt acattaaaat ttggaggtaa aatatgaaaa aattattgtt
     2461 tacagggttg ctggcaagtt cagcattgtt tttattagga ggatgcggat caagtaatgc
     2521 aagcgatgcc agttcaagtg cagcaaattc ttctgaaaaa acaagtgatg gaagaaaaaa
     2581 tattgatttc tggtcctttt ggggatccgg tgcacgaaaa gaagctatcg aagaaatcat
     2641 tgaggatttc aatcaatcac aaaacgagat ccatgtaaat tataagtatc agccatgggg
     2701 tgacatctgg actaaatctt tatctgcaat aacagctgga aatgcacccg atgttattgt
     2761 acaggacata aattccgttg cccaaagagc agaagcacag caagcaacta atctctccaa
     2821 atatgtagaa aaagacactt ctaaggattt ttatccgcag ctttgggata cagtagaata
     2881 tgaaggtgat ccttatgcga tcccattcaa tacagataca caagtcattt tttataataa
     2941 aaagatattt aaagaagctg gaatcagtga acaagatcta ccgacaactt gggatgaatt
     3001 agaagaaaca gcacgtaaac tagataaaaa agaaggaaaa gaatttacac gcatcggttt
     3061 ttatccatta tggaatttag gggctgatgt atgggcgcta aacgcagata acgggactag
     3121 ttggtttgac aaagatgaaa aagtcaagat tgatactaaa aataaagtgg aagccttgga
     3181 gtggatccaa gactggcaag attattatgg gaaaaatacg atcaataaat tagaagccga
     3241 atttgggtct ggtgtatctg atccatttat ttccggatta gtagccatgc gtgcccaaaa
     3301 tatcaattat tactctagtt tgatggaaaa tgcaccggat gattttgatt ttggcgtgat
     3361 gcagctccca gaaaaagaag aaggttcagg ccattggtcg tggggtggcg gattcgtctt
     3421 ggaagtacca tacggagcaa aagatcctga ggcctcttat gaattcatca aatatctttc
     3481 ttctccagaa gtacaagaaa aattcggcga aaaaagtttt gatatcatgg cgagcaagac
     3541 agccaatgag aatttagtga ataatgacaa cctaacggat aaaggaaaaa tgatctatca
     3601 aatggcggac gaaaacttta aaaatacggt gatcacacca gttcctttag atgctccgga
     3661 ttacacgaca ttagtgaatg aacagattga ccagattctt ttaggaacaa aatcaccaaa
     3721 agaaggattg gcagatgcac aaaaagcagt agaaaactta gtcaagcaaa atcaataatc
     3781 catttttgac tgtctaggta cagaggaggc aagaaaattt gaagcgttta tctatccgaa
     3841 agaaaaaaga agcaagatgg gcctatttat ttatctcgcc ttttattatc gggttcttgg
     3901 tatttatgct agggcctatg cttttttcag ttattggtag tttcaccgat tataatttga
     3961 catccaagat gaatttcatt ggtctagcaa atttcaaaag aatgttcgtt cacgatgatt
     4021 tattttggaa atcgttatac aacaccgttt atttcgtcat cttcaatgta ccgctgacga
     4081 cgattttcag tgtgttttta gccacagtac tcaatcaaaa aatcaaagga atcagtgtat
     4141 tccgtacagc tttctattta ccggcagtct tatcaggagt agcagtctac atcttatgga
     4201 tgcagctgct ttctccatct gcaggattga tcaacacatt tctgggctgg ttccatatcc
     4261 ctggaccagc ttggctgttt gatccacaat ggacgaaacc tgcgttgatt ttgatgaaaa
     4321 tatggagttc tggaggagca atgctacttt acttagctac attacaaaat gttcctagat
     4381 ctttatatga atcagcagaa atcgatggag caggcgtttg gggaaagttc aaaagtatta
     4441 ctttgccatt aatcactcct attatttttt acgatgtgat cacctctacg atcggttctt
     4501 tccaaatttt tcaagaagct tatgtcatga cgaaaaatgg aacaggcgga cctgcaaatt
     4561 ctttgctttt ttataatctg catatgtgga acaaggcgtt cgtagctttt gatatgggtt
     4621 atgcgatggc tatgtcaatg atcctgtttc tgatcgtgtt ggttctgacg tttgtcaatt
     4681 tgaagcttgc acctaagtgg gtctattatt ctggaggtga cggcaaatga tggattattt
     4741 caaaaagacc agtgtgaatc ggcaaacaaa aaaatactaa gttatgtcct catgacagta
     4801 ataggaatca ttttgatcat tccattgctt tggatggtgt ttacttcatt gaaaccaatg
     4861 gaagaaatcg ttcgttatcc tccgaccttt ttcccagaaa aaatcgtatg ggaaaactat
     4921 ctggatacga ttgccgcctt tcctttttgg cgctatgcaa gaaatacatt attcatcact
     4981 gtgctagtag tgatcggtaa tgtcctttca aattctttta ttgcttatgg atttgcgaag
     5041 cttgattttc ctgggaaaaa gctgatgttt gctttggttc tttctacgat gatgatcccg
     5101 gggtttgtaa cgatgatccc tcaatatgta ttattctcca agatcggctg ggtaggtact
     5161 tatttgcccc tgatcgttcc atcgttcttc ggcaatgcgt tcaatatatt tctgatgcgt
     5221 caattttact tatcgatcaa taatgaattg atcgaagctg ctgaaatcga cggagcgaat
     5281 catttgtata tttggagtca tttgatgctt ccattgacga aacctgcctt gattaccatt
     5341 gcgatcaatt ccttcaacgc agcttggaat gatttcttag gtcctctgtt atatatccaa
     5401 gatcaagaaa aatacacgtt acagattggc ttgcaagttt ttcagaacca agcaacgaca
     5461 caatggaatt atctgatggc aggagcaaca cttgtcttga tcccgacgat tttgctgttc
     5521 ttttttgcac aacgttattt cattgaaggg atggatttga caggaggttc aaaaggatga
     5581 tgaatcgatc gattggtaaa aaagccaatg attatttctt agaaatcatt gaatggattc
     5641 cacgtcttat ccatttacag ctactatggt tcctgtgcgt cttgcctgtt ttcacattag
     5701 gaactgcaag tcgaacagtt ctgtatatga tttatcatta caaaaaaatg aagagaaaaa
     5761 gctcggttca ctattctgga aaaagtttcg tgagaacttc tctctttatg gaaagcaaga
     5821 tagtttagca agtgtttatt ttcttcttct attgatcgat tatcagattt tccgctattg
     5881 gggtggggga attggctata cattgatgta tactacgttg tttttgcttc tattaagcgc
     5941 gcttctcttt agttacaaaa tcttgcttca actggaacaa aaagaagctt cttggattac
     6001 tgcatttttc ttgtttttta atgatttcaa aagcgcgctt ttctatttgg caggcacgtt
     6061 gatccttctt gtcgcattct ggtttgcagg accaatctat tttgctttat taggtttcag
     6121 tttcttgttt tattttcaag tcctgctttt tatccatcgt acgcaagaaa aggttcttaa
     6181 aaaggaggaa taacatgttg cgtttgattg ctaaaatcga tgatagtatt atcaacaagt
     6241 tcgccaagca acggaaaacg gatagtcgaa aaatcaattt tttgaaattt atcagtaata
     6301 tctctaatgg tggttttatt tggttgctcc ttgctgctgt tttagtcttg atacctgata
     6361 ctagagaata tggtctagcc attcttatcg gcagtttgat ctattttttt gttggtttgc
     6421 tttttatcaa aaaaacgaca agacggaaaa gaccttatga aaaaaatcgt cggcttgtcc
     6481 tttacacaag taaaccgaag acttcttctt ttccttcagg acatacagcg ctagcctttt
     6541 tatcagtagc tgcgttgttc gaattaagtc cagttgcagg aatattagcc tttttatttg
     6601 cttgtttgat cgcttattcg aggatttttt tacttttaca ttatcctact gacattttag
     6661 gaggagctat tttaggaagt gttattggca tcctatgcgc tgccgttatc tgaattaatt
     6721 gtacgaataa agcatgagtg cgtaaatgcg catttgtgct ttattttttt gtttcgagga
     6781 aagcgtagtt gtttattcat ttgtcttaat aacttaagtt ttgttaaatg agaacgttat
     6841 aaaattt
//
LOCUS       DAZKNH010000114         6822 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000114 DAZKNH010000000
VERSION     DAZKNH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.114"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..374
                     /locus_tag="ACG8MG_002385"
     CDS             <1..374
                     /locus_tag="ACG8MG_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376388.1"
                     /translation="LTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGR
                     CRTCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLN
                     QSIIGVKTLAYLSGMGRNRRF"
     gene            692..1282
                     /locus_tag="ACG8MG_002386"
     CDS             692..1282
                     /locus_tag="ACG8MG_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HFQ0376389.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            1309..2619
                     /locus_tag="ACG8MG_002387"
     CDS             1309..2619
                     /locus_tag="ACG8MG_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0376390.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            2616..3335
                     /locus_tag="ACG8MG_002388"
     CDS             2616..3335
                     /locus_tag="ACG8MG_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 32 protein"
                     /protein_id="HFQ0376391.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            3350..4435
                     /locus_tag="ACG8MG_002389"
     CDS             3350..4435
                     /locus_tag="ACG8MG_002389"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0376392.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            4571..6103
                     /locus_tag="ACG8MG_002390"
     CDS             4571..6103
                     /locus_tag="ACG8MG_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0376393.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            6281..6502
                     /locus_tag="ACG8MG_002391"
     CDS             6281..6502
                     /locus_tag="ACG8MG_002391"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0376394.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT         2396 a          736 c         1308 g         2382 t
ORIGIN      
        1 cattgacaca taattttaga gataatgagt tcgattgtaa tattgaatta gttaaaaaaa
       61 ttgaaggcga tttaagctat atattagaaa atgagatatt tgtaaacaaa acaaggagac
      121 ttttgttggg acgctgtcgt acttgtattc aaaaggcagt tagatatgct agttttaata
      181 aagaatttga tttaaactcc tatttgtcta agatacttat gaacgatgag ttgattgcct
      241 gtattcactc atacccatat aaacaaaatc caattaaaca acgaatattt aactattttt
      301 tactaaacca atccattatt ggggttaaaa cattagcata tttaagcgga atgggtagaa
      361 ataggagatt ttaatcataa aaaactgtac tatagtgaat ttttggagat ttatattcca
      421 gttatatatt aatgattgtg atacttgtac tattgtaaaa attcgttagg tttaaataac
      481 tggaaatatc ggaaaatggg cattatacat cacggacatc ccttcttttg gaaaaaatgt
      541 tacgattttt ataagtaata ttgtatccgg tggaatcaac gatgaagata acatatccat
      601 cgttgtagat tcagtagcat tgaatgatgc tcttgttgca agaaatccga ataaaattgt
      661 taagtataaa ttgaattaat aggagatatg aatggaaatt agcagtaaag aatatttaaa
      721 agcggttctt cgtatggaaa agaatctata tatagaatct aaaggacaac acacaaaatt
      781 actaaaaata atgaaatcac atccggacta caacgcttgg cggtatactc gtcgaatgag
      841 aattacatct tattattatt caaagagaaa gaaaaatatc atttatgcga ttatgtatat
      901 tgtgtcttgc agaagaatga atagtttggg aagaaaatgt ggtatagagt gtggtgaaaa
      961 tgtatttgat gaaggtgtaa gattttacca tacacaagga attgttatta atggtaacgc
     1021 cataatagga aaaaattgtt acttatatgg gaataactgt attggaaatg atggaatcga
     1081 tcctaagtgt cctgtaattg gaaataatgt acgtgtatgc gtgggtgcaa agattattgg
     1141 taatgtaaaa atagctaaca atgtagttgt tgcggcagga gctattgtta tcaaagattg
     1201 tctagagaat ggtgcaattc ttgcaggtgt accggcaaaa ataatagggt atgttaattt
     1261 aacaaaagta tttttagaat aatttttagc gaaagaaggt gaataacgat gcgtatttca
     1321 ataaaaagat ggtcaggagc agattggaca ttgttcacgt tggcattttt tgcaatctcc
     1381 atagttcttg tatatacttt taacgcccca agagcaattt tatatttggg agatataatt
     1441 aatatctttg tgttattaaa tgcacttagc tttagacgca gaaaaggaat atatgctagc
     1501 aaaaatgaga tgctgatagt tttgttttta ctagtttcta tttgtagctc agttattaat
     1561 ttagaaaata taggattgat tatctggggg atacgaaata acactcgtat gttcatagta
     1621 tatataaatt gtgcaacata tttaacaatt aaagattact ataaagtatt cgctataatt
     1681 gaaaaattat tctggatttc actgccactt tgtttaattg aaagattcat ggtcacctat
     1741 ccaactggaa caattgtagg agatatgata ggtggagttt tttggaatta ttcaggttgt
     1801 aatatgccat tgaatttaat tttgtgtgtg acagttactg ttgttacaat cggatatttc
     1861 aagggagaaa aaaatttcat ccattttatt ttatgtgttt gtgcagcact ttttatgtct
     1921 gcattagctg aattgaaagt ttttattcct gaattattta taatatttgt tgcaatagta
     1981 ttcactttaa aagttgattt taaagtagtg atcaagatat tatcatgcgt aatacttgtg
     2041 tcgatagtat tatccttttt tataacaata tttattacat tgaacgataa tggacaaagc
     2101 tatgctgata atttcacaat tcaaggtctt ttagaatatg cgtcaagaga ttctggatat
     2161 gatggtgttg gtgatttaaa tcgaataact ggtatctcta gagtagtaga tgaaatattt
     2221 aataataatt ttagattagt cctaatggga gtaggcattg gcaatgcaga gtttaccaat
     2281 ttttttgtca gtgagtttta taaatcgttt ggtaatcttc attatcagta ttttcatcat
     2341 atttggttat ttatagaagt tggaatatta ggagtttcga tattcctatt ttttataata
     2401 aagacatttt tcaatgcaaa aagatctttt atgaaggata aagatgcaag tcttgttaaa
     2461 gttattgcaa ttatgtcggt attctttttt gtgtataaca caacacttag gggtgaaaca
     2521 acggggccac tgatttatat gattttagcc atgcctgaag ttgtgaaaag gaaagagcat
     2581 tattatagtg aaaaaaatac aaaaggagag atattatgat tcctaagaag atacattact
     2641 gttggttcgg gggaaatcca cttccgaaaa aagatagaaa gtgcatagaa agctggaaaa
     2701 agtattgccc tgattatgag attattgaat ggaatgaatc aaattatgat ttaaataaga
     2761 accgttatat ggccgatgca tataaagaac gtaaatgggg ttttgttcca gattatgcaa
     2821 gatttgatat tatttataat catggtggat tttatttaga tacagatgtt gagctaatta
     2881 agtcgttaga tatacttcgt gaaaatcatg cctatatggg atttgaaggg gaaatctggg
     2941 ttaatggcgg tattggtttt ggtgcagaag aaggacattc tgttattaaa ggtttaaggg
     3001 atatttacga ttcattaagt ttttatcaaa atgatggtac attgaacttg aaaccgagtc
     3061 cgtattatat tacagattat cttgaatcta ttggattggt tcgcgataac agcgaacaaa
     3121 taataaatgg cctgcatatc tatcctacgg attattttgc tgccaaggat tatgatactg
     3181 gaaaaattta taaaactagt aatacaataa gcattcatca atataatgca tcttggaaaa
     3241 cacctaaaca aaaatttgtt tatagattaa aatcaacaat tggacaaaaa aaatttaaca
     3301 ggttacttgc actgaaaaaa tttttacact tataaaggag aaaagtactt tgaagaaaat
     3361 ttgtttcatt agtggtgtaa tttctcgttc tgggggaact gaaagagtag ggagtattat
     3421 tgccaatagc ttgtctgatt taggttatga tgtcactatt ttaagctttt ggaatagtgg
     3481 agagccttat tttgggttag ataagacgat taaggttgat tatatgttaa atcctaaaac
     3541 ggagggaaaa ttatatagaa cttatttata tccggtttgg aaattgcaca agtatatatg
     3601 caaaaatgaa tttgatatag taattgatat tgatactttg ttgtctttgt atactacaag
     3661 ggcaattaaa ggaacaaagt gcaaattgat ttcttgggaa cattttaatt actggacaat
     3721 ggaaatgctg aaagaaaaga agagattcaa ggctaaaaaa ttgataaaaa aatatgcaaa
     3781 gaggcttgtg gttctcacag aagaagatcg tcaaaaacat attgatgaat atagtttatc
     3841 agaagatttt gttataacta tgccaaatcc gtgtatatct aatatcgagt acgaatatca
     3901 ttatgataaa aagagatttc ttgcagtagg gcgattagtg gaagagaaaa attttgctga
     3961 catgataagt gcttggaaaa ttgtagaaaa aaaagtagat gattgggatc ttatcattgt
     4021 aggaaaaggt gaattagaaa aagatcttcg ccagcagata aaagatttaa atctgcaaag
     4081 agtaactata gcagggcatt cagaccatgt ggaggaatat tataaatctg catcttgtta
     4141 tgtgcttagt tctgtttatg aaggatttcc tatggttata ttagaagcac agtcttatgg
     4201 attaccagtt atttcttatg attgtaaaac tggtccgaga gatttggtac atcataattt
     4261 taatgggatg ttggttgaag ataagaatat agaccaatta gctaagagca tgataatgtt
     4321 tactaagaat actgatttag caatgagaat gtcactgaac gcatataata atgtacaaaa
     4381 atttaatttg aaagaaatta caaaacaatg ggttgctttg attgaaaatg tatagttttt
     4441 tgtggttgtc caaacatgaa aattagcgta attaatcaaa gagctagaag aaacctatta
     4501 catttcaaga ttttagaaat tatatagtat taggtaacaa taaaaaatag aaagtttgga
     4561 gccatatgat atgataatta atcgtaaaca aaatgctaaa aggaatataa ttactggaac
     4621 aatttttaag ttatatcaga ttatcattcc cttttttatg cgtacactta tgatttatca
     4681 gatgggaatg gagtatgctg gattgaacaa cctgttcaca tctatatttc aaatgttaaa
     4741 tttagcggaa ttgggaatag gtgctgcact aactgtaagt atgtataaac ctatagcaga
     4801 agacaataga aaagagattt gtgctttact gaacgcatat aaaaagtact tttcaattat
     4861 tggatggata gtgtttacat taggattgtt atgcattcct tttttgagat ttttagttaa
     4921 aggttcgatt cctggaaatt taaatatgac agttttgtat ttaatgtatt tgataaatac
     4981 tgttttgtcg tattggttat tttcatatag aaagagtcta atatatgcac atcaaagaaa
     5041 tgatgtaatt aacgtgtcta tgctaattac ttttacattc caatttgtaa tgcaggcata
     5101 tgttctgatt ttcttaaaaa attactattt gtatttattg gcttctattt tgggacaaat
     5161 agttcttaac ttaatgtgtg ctgttgtttc tcaaaagatg tatcctgatt atactcctgt
     5221 tggtgagtta gagaaagaaa ctattgacga tattataaaa aaagtaaaag gattagttac
     5281 aaacaaggta ggcggtacaa tattacgatc ttcagattca attattattt catctttctt
     5341 aggtctttca gtacttgcga tttatcagaa ttattacttt attatttctg ccataatgag
     5401 tatatttgca ataatttatg aatcatgtat agcaggtata ggtaacagtc ttattgtaga
     5461 aagtcaagaa aaaaattata tagatttttg tgatattaca acaattatag gttggtttct
     5521 ggcaattagt tgtgcatgct ttattacatt atttcagccg ttcatgatta tctgggtggg
     5581 aagtgataat cttatgagta tttcatttgt tatatttatg atagttcatt ttttcttata
     5641 tgaaattgat cagttaattg ggttatacaa agatgctgct ggaatatggt acgcagacag
     5701 gtttagacca ttaattacag caatgataaa cattgtatta aatatcatcc tcgttaattg
     5761 gttcggatta tatggagttt tggcggcaac cattgtttca gtattgttgg ttgatttacc
     5821 ctggttatta tataatgttt tccaaaatat atttaaattt gaatcggcag gtaaatatgt
     5881 ttggaagctc ataagtttgt gtgtaatagc ttttgttgct tcttatttaa gttattttgc
     5941 ttgttcgtat gttaatatcg gaaatttcaa agtgataagt ttagtgataa atttagttat
     6001 atcggttgtc atatcgattt tagttattct tctgtttaat atccgtaata agaagtttaa
     6061 gtatattttg aaagttctta cgtcggtatt ggtaaaaaga tgagaagaga catcagaatg
     6121 atatataaag taactacaat ttttaggctg ccgaataggc gaacgatgta tagtttttgt
     6181 tccaacgaag actcttgttg aaattggaga tgatatgaag gagttacaat tttgactcat
     6241 gtttatgatt gatcggtttt aaaagtagta tggagaagct ttgggatcat cggagaggat
     6301 caaaataggt aataatgttt ttataggaat gaagtctaga atactaaagg gtgtacatat
     6361 aggaaataat gtagttatag gtgcaaatag tttagtaaac aaggacgtat cagacgattg
     6421 tgttgttgca ggaaatctag caaaagtaat tatgtcccta gatgaatatt ataaaaagat
     6481 aaacaatatg aagaatccgt aaaacttgtg aaactatacc gagaataata tgggaaaaat
     6541 attgatgaaa aagcattata tgagttttta gggctgttta cagatggaga tgacaatttg
     6601 tcgaagagtt gggagcaatg caaaatttag taggggatta taaacagact gacaaggtat
     6661 ttaaagaaca tgcacaagta tttcataata ttgaagaatt tttgaatact gtgagatagg
     6721 agttgctaca ttgagaaaag tatgtatttt tccaatgtaa aagattacga gtttgaaaac
     6781 gacaccataa tattattttt taagaagata ttatagagct ta
//
LOCUS       DAZKNH010000115         6572 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000115 DAZKNH010000000
VERSION     DAZKNH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.115"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG8MG_002392"
     CDS             complement(99..1229)
                     /locus_tag="ACG8MG_002392"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFQ0376395.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG8MG_002393"
     CDS             complement(1878..3080)
                     /locus_tag="ACG8MG_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0376396.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG8MG_002394"
     CDS             complement(3140..3343)
                     /locus_tag="ACG8MG_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0376397.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACG8MG_002395"
     CDS             complement(3485..4552)
                     /locus_tag="ACG8MG_002395"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0376398.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG8MG_002396"
     CDS             complement(4610..4717)
                     /locus_tag="ACG8MG_002396"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0376399.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG8MG_002397"
     CDS             4880..5692
                     /locus_tag="ACG8MG_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0376400.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG8MG_002398"
     CDS             complement(6066..6257)
                     /locus_tag="ACG8MG_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376401.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1994 a         1329 c         1028 g         2221 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata ta
//
LOCUS       DAZKNH010000116         6287 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000116 DAZKNH010000000
VERSION     DAZKNH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.116"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..690
                     /locus_tag="ACG8MG_002399"
     CDS             190..690
                     /locus_tag="ACG8MG_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0376402.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="ACG8MG_002400"
     CDS             787..2634
                     /locus_tag="ACG8MG_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0376403.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="ACG8MG_002401"
     CDS             2637..3533
                     /locus_tag="ACG8MG_002401"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0376404.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="ACG8MG_002402"
     CDS             3583..3972
                     /locus_tag="ACG8MG_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0376405.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="ACG8MG_002403"
     CDS             3959..>6287
                     /locus_tag="ACG8MG_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0376406.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DAZKNH010000117         6167 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000117 DAZKNH010000000
VERSION     DAZKNH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.117"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(155..1606)
                     /locus_tag="ACG8MG_002404"
     CDS             complement(155..1606)
                     /locus_tag="ACG8MG_002404"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0376407.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVRTVLQRGGSM"
     gene            complement(1927..2691)
                     /locus_tag="ACG8MG_002405"
     CDS             complement(1927..2691)
                     /locus_tag="ACG8MG_002405"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFQ0376408.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(2719..3417)
                     /locus_tag="ACG8MG_002406"
     CDS             complement(2719..3417)
                     /locus_tag="ACG8MG_002406"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFQ0376409.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3429..4208)
                     /locus_tag="ACG8MG_002407"
     CDS             complement(3429..4208)
                     /locus_tag="ACG8MG_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFQ0376410.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4224..5168)
                     /locus_tag="ACG8MG_002408"
     CDS             complement(4224..5168)
                     /locus_tag="ACG8MG_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0376411.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5214..5993)
                     /locus_tag="ACG8MG_002409"
     CDS             complement(5214..5993)
                     /locus_tag="ACG8MG_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376412.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1694 a         1283 c         1012 g         2178 t
ORIGIN      
        1 catatttaat tcataaatat aaatcttcat tttaaatgta tgcccaaaaa tttatctgta
       61 atgcgtcatc aaaatctaga ttccaaatct ttatcaattt ttatccactc attctaccac
      121 tttagtaata ttttctattt ataacacttt cgtcctacat tgatcccccc ctctgaagta
      181 cagttcttac tgtcttaaat aaaattttaa aatctaaccc catactccaa ttttttatat
      241 attccgtgtc aagttttgct acttcttcaa aatcagtaat atcacttcta ccacttacct
      301 gccacattcc agtaattcct ggcttaatag caattcttgc acgatgatga taatcatatt
      361 cttcatactc tgatttaagg catggtcgaa atcctataag gctcatcttt cctgttagaa
      421 cgttgaaaaa ttgagggaat tcatctaaag acgtggatct aataaattga ccgattcctg
      481 tcttttttgt tccatctgga agtattttat ttccgataat tcttggatca aactccatct
      541 taaacataag attattgctc atcttatttt ctttcattaa ttctactttt ctttcttctg
      601 catccatata catacttcta aacttataca tcttgaacgg tttaccattc tgacctatac
      661 gttcttgtgc aaagaaaatt gggcctggtg aattaatata aataagtgga gcaataaaga
      721 taaaaataat tcccgttgca atacatccaa caatacctgc aagaatatca attgttcgtt
      781 ttataaatgc ctgttgagtt gtcataacat tgatacttgt tgtaagtact gtatattctc
      841 ctatacgacc aatagattgt tttcccaaag cactatctga tacttttgca agattcaaat
      901 gtacagtaag gcccatttca gagcatcgtt caataaattc tttcatacac gaagtacttt
      961 tattcaaaat aataaagact tcatctaccc atccctgaca taagtaatcc gctgcatttt
     1021 tggaatcaga aacaactgtc actccctcaa catcttcacc aatcatgctt ttatctatga
     1081 taatgacacc atttatttta tacattttat agttatgctc tttcacatta cgaataacta
     1141 cttcagcaat actactagat gtaactataa taagtgattt gttcccctca tttttcattt
     1201 tatgtttcaa gtaatgtttc cataagaggc gagttatata tgtaattaca acgtataaac
     1261 ctcccatcaa gaaaagaacg actctagaat aatcatttcc accctgaaca ctaatgagat
     1321 acaaacttcc taacagcacc aacataatcg cttgctttac agaagaagca aattctatat
     1381 agtaccctct ttttaataca cctctatatg cttcaaacat gaaaataact aaaagatctg
     1441 ccagttcaat gaagactgcc atgttttgat acattggaat cacataagga ttccaatcta
     1501 cctggcgtaa gacatacgat agataaaaag caagctggat acacaataaa tctaaaataa
     1561 taaaatcaat atgtttaacc catccccttg aacttttctt gtacattcct ttttactccc
     1621 ctctttttaa taaaataaat tgtattactt gttttctctc ctaaatatct ttctgaagga
     1681 ttattgactc gacaaattgg cttgtggata ttcttatata tagaacatcc gtaagccaac
     1741 ttaaaactcc ttaacaaaat ttttttactc atattgtaga tcaaaacaag tatccaagaa
     1801 atcatatttt atttcttttt tctgcttact caacaacagc taaaagaaaa atcatcataa
     1861 acaaattcaa ctaagccaac gatccccttg tgaaatcaag ttttttcttg atttacgaat
     1921 aaagagctaa aataaaccaa acttcttctt ttttacctct tcatagactg gataactgat
     1981 ttcatccccg ttgatcagat cctttgcgac ttgtttcatc tgtactactt tttccttacc
     2041 aaagtccttt gcaatctgct catagcactc ttttaagtaa aaggtgcgct tagagacccc
     2101 atgggcatca gaagcagcca tctgcacgag attatgtttg accatcgttt tggctgtttt
     2161 ctggatgtct ttgccaaata ccccaacata acttggcgca gtgagttggg ccaaacatcc
     2221 catatctagg aacggaagca aatgatttgg gtcttcgcga aaatgcacgt tgcgttctgg
     2281 atggacgatt actggtgtct ttcctagttc taacaactca aaaaaaagcc gttctgcata
     2341 taacggcact tccagtgtcg gaaattcgat cagaagatat gtatcatcta aatctgtaaa
     2401 taatatttca tctcggtgga tgtcttcgat gagcgttccg gtaatacgta cttcttgtcc
     2461 ttccaataac gtcaaaggca actgtctttt ctctaattcg gcttgtaacg aagcgactag
     2521 agaaatgact tgtgattttt cattgctgta ttttccatta ttgtggtgtg gtgtgcataa
     2581 aatatgcgtg atgccttgct gaatcgcttt ttcagccatt gcaatacttg cttctaagtt
     2641 ttctgccccg tcatcgattc ctggtaggat atggcaatgt agatcgatca tttctcttgt
     2701 ctcctctctt cttattcctt aatttccgta gtaataacca gcatctttgc tatgttctgc
     2761 tccgttgtac acaacaccta agactcgtgc ttgtaccatc tccagcagtt cttttgcttt
     2821 agtcaaggat tctttacgtg acatattttc acgtacgacc aataaagtgc catccgtttt
     2881 taaagacatg atctgtgcat ctgttacggc tactactggt ggcatatcga atatcaccac
     2941 gtcaaataac tgtcttgctt cggataggat ctgatccatt cttggcgagc ttaacaattc
     3001 tgatgggttg ggtggcttag gtccactagg caaaatcgac aagttctcga ctggcgttcg
     3061 ttggatcaca tctgctacac ttcctgatga actaagtgct gtacttaatc cactggcatt
     3121 gttcagctgg aatgtcttat agacgacggg tttacgtaaa tctgcatcga ctagtagaac
     3181 tcgttgacct gatttagcaa aaacgactgc aatgtttgct gcagtggtgg atttcccttc
     3241 tccaggacct gatgaagtca cgacaatcgt tttgatttgc tgaccagctg cggaggcaaa
     3301 ctggatattc gtccggatcg tacgatattg ttcggcgatt ggtgaagatg ggtttgttaa
     3361 tgtgatcaaa ctgactgcat gagtttgtgc tgctttttgt ttctgtgttc gtgccatttt
     3421 tcttcctcct agactcttga gcggcttctg cgtgatgggg ctgttttatc gtttgtagct
     3481 tttggtcgtg caggagctgt tcctttttta cggataccag cgttcaattc tttcgctgac
     3541 atttctggaa ctgttcctaa gattgggaat cctaatgtat ctgtcacata tttttcatct
     3601 ttcactgtcc gatcgagtaa ttccaataag aatgccagac ctacacctac catcattcct
     3661 aacattaagc cagtcattaa attcaattta ttatttggcg aaacaggtgt cgtattggca
     3721 actgcacctg aaatgatcga gattttatca acattcatca catcttttgc gttctcttgg
     3781 aaaacttgtg aagttgtgtt agcgatctgt tgagcagcca ctgcattcgt acttattgcc
     3841 tgaatcgaga acatttgtga attttctgcc tgattaactg agatggcatc tttgatttgt
     3901 tcagcagtca ttttcacatt gtcttctgtt tctaaacgat ctttgacttc attgagcact
     3961 aaatcactga cgatcatatc tttatacgta ttgatcattt gcaggttcgt attgacatca
     4021 ttgacatttg cagtgtctga ttgtggcagt gtcacgatca attgtgcttg tgaactgtat
     4081 tttggtgtca agatgaaaaa tgttacgatt cctgagatgg ccaagccagc aaacattgag
     4141 atgatgatta ttaccaagtg tttgcgtaat gtttcaaaaa tttctttcaa gctgattgtc
     4201 tcttccattt tttctccact ttcctattta ttctctagat tcatttgatc ttttaattct
     4261 tttttcacac gatcaagttc ttgatcactg actttttgat aggagatatt atcttgcatg
     4321 aagccttctc cttgcagctg atcttgctta atcgtaccaa atgcctcccg ataatctaaa
     4381 gcaatttttt tcatttcatc aaagttcatg tctgttttca tattcgactc tagagcattc
     4441 aaaacggatt gatagttgct gacaccgttt agactcaata cttttttcgc tacgccttct
     4501 acgatctttc tttggcgccc ttgtctaccg taatcaccat ttgggtcttc ataacgcata
     4561 cgagaataag ataatgcctg atccccgttc aatgtaattt taccgattgg aaagtcatag
     4621 ccatcttgtg agaaagtgag gtcattattg acttcgatac caccaactgc atcaacgagt
     4681 tcctttagtc ctttcatatt gatagagaca tagtgatcga ttgggatgtc tacatatttt
     4741 tgtacggtat ccatcgccat ggcagctccg ccgaatgcgt aagcgtggtt gatcttgtct
     4801 tttgtgtcac gacctacgat gtcgacatac gtatctcgag gaatgctgac gatcgttgtc
     4861 tgtttgtctt caggactgac tgtggcaacc atcatcgtat ctgatcttcc ttgatcgacc
     4921 cgacctaaat ctccagtatc aatccccatc aatagaacag agaaagattc ttgtttgtcc
     4981 agatcaactg gtttcgttcg ttctccttgg gttcgttcca ctgattcgta agttttctca
     5041 atagaatgtg agagatccag atataatttt gctcctactc ctacaattgc taatactaat
     5101 acgataagaa ctgctaaaat aatcaaaaat atttttttcc ctcttgattt tcttcttcgt
     5161 cttgccatag tttcctccgt ttgctaacct tgcttgaatc aacgttttaa ctgtcaatag
     5221 cttatattgc tatagcccat ttgctgagca acagtttgtg cttttgttgc gtatgcggct
     5281 cttatttttt cattccggat ctgctctatt tgactgagcg ccgtcaaata ttgatcgtaa
     5341 gtagcggtag ctgttacttg accgtctttc agcatcgtat cgaatagctg atctagttgc
     5401 gtgacttgtg caatttgggt atcagcgaat cctagatatt gcataacgac tgttttccaa
     5461 gcactatcct taaaaaagct catattttca cgtacatggg cgacatctgc acttgccaac
     5521 ttttctttga tgatgacatc atccacagct tgctgccagt ttgatacgtt tttagtaaat
     5581 aatttattga cagccgtctg gatcttcagt tttgattctg catcctctaa ctgcgttaag
     5641 actgctttct tttcttcttg gatcgcagca gcttctttag gtaattctga ttccttgatg
     5701 tcataatcat ctgccgaaag tttgatagaa gatagtttat gtgacaaatt tgaaactgtt
     5761 tcttctgtta tttcaggatt taggaaaatt ttttcttttg tactgtacag cttatctact
     5821 tcttttgata aagaggtcac ggtgtctttt tgattggtga tggcagattc tgcgtaattg
     5881 atccgttgat gatattgccg ctgattattg agatggtatc caaacaagca aatcacaata
     5941 gcgcctgtgc ctaccgccac agtagccaat aacttgacga acggcttttc catagattca
     6001 ctcctaaata ttgatgatag ataagtaaag atgtgattca ttcacatcag aatgagcgct
     6061 ttttatttta aaagcataat taaactaata tattcataga aatatttata aacaggtttt
     6121 ccctatataa tcataccaca tacagttttt ttgactaggg aaattcc
//
LOCUS       DAZKNH010000118         5957 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000118 DAZKNH010000000
VERSION     DAZKNH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.119"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(254..541)
                     /locus_tag="ACG8MG_002410"
     CDS             complement(254..541)
                     /locus_tag="ACG8MG_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0376413.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="ACG8MG_002411"
     CDS             complement(561..785)
                     /locus_tag="ACG8MG_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0376414.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1240..1410)
                     /locus_tag="ACG8MG_002412"
     CDS             complement(1240..1410)
                     /locus_tag="ACG8MG_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376415.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1425..1958)
                     /locus_tag="ACG8MG_002413"
     CDS             complement(1425..1958)
                     /locus_tag="ACG8MG_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0376416.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="ACG8MG_002414"
     CDS             complement(1979..2923)
                     /locus_tag="ACG8MG_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0376417.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3560..3646
                     /locus_tag="ACG8MG_002415"
     CDS             3560..3646
                     /locus_tag="ACG8MG_002415"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0376418.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3767..4456)
                     /locus_tag="ACG8MG_002416"
     CDS             complement(3767..4456)
                     /locus_tag="ACG8MG_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376419.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4463..4741)
                     /locus_tag="ACG8MG_002417"
     CDS             complement(4463..4741)
                     /locus_tag="ACG8MG_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376420.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4701..5615)
                     /locus_tag="ACG8MG_002418"
     CDS             complement(4701..5615)
                     /locus_tag="ACG8MG_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0376421.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5597..5956)
                     /locus_tag="ACG8MG_002419"
     CDS             complement(5597..5956)
                     /locus_tag="ACG8MG_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFQ0376422.1"
                     /translation="MASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQMSVPAF
                     VKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPGDAQRLSE
                     NLKIMQKELQKIWQQLK"
BASE COUNT         1573 a         1092 c         1004 g         2288 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtgtatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa
     3421 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac
     3481 accacacaag tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     3541 ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc ccccttctta tagaagtttg
     3601 tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg
     3661 acatcaacct gaaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtatctaa
     3721 tgtttgatca tttgcatgtt tagtttaaca tgattgccga tcaaattcaa ttttgctgtt
     3781 gctgatctaa ccattccttg acacttttta gataatttaa tgccgtttct tttttcgttg
     3841 gattttgtat gttcgttgct tgctgaagca gtgctttata acgttcgtag gtaactgatt
     3901 gttgccgttc ttttcgttcc tgttcactag ttgaaaaagg gattgtatca ctgaataaat
     3961 tataacaagc ttcttctgtc tgcgtatctt taaaatattg cacgtctttt ttggttcgct
     4021 catagttgtc ttgaacctct ttttgctgtt tttctggcaa gttttgataa agacgagtga
     4081 gtttttccat gtttttatcc gtcaaacgtt tttcttgatt cacaaaaata tcgttcattt
     4141 cctcataaca agtggtaaac tcttccattt tttgtgttaa cacttgctcc tttttctttt
     4201 tatcacatgc tgcttcatac ttttgagtga tatcccctaa taaaatactt cttgaaatac
     4261 gtttatttcc ctctaaaacg ccgaataaac cacttaaaac gattgataat gtaaatataa
     4321 cccctggcaa taaaaacctc ttaaatcgaa ttttagagcc ttttaatgaa tatcttgttt
     4381 gataagcctt atagctaagc caaataattc ctaaaatact ggcaaaaatg agtagatagg
     4441 gtaaatagaa tgccatttct atttattctc cccataccag ccaaaaggct aacagtgtca
     4501 tgattaagca acataagggg gcaagaatat ccattgatcg ctgcataatt acttttttct
     4561 gttctttatt tcgctggttt aatagcgcat ggtggaattt tttgacttgt gaatcaattg
     4621 tagtttgtat tctttctttg gtataaggtg caagaaaagc acgttgtttc tttatttcat
     4681 tttctatgtc ttgtaacgtt ttagaacgat aattcttgtt ggtagtctgc tcctctttca
     4741 acgtgttggt catggtttct tcgagtcggt ctaatttccg gttgttcagt cgtaagagtt
     4801 cgttgttctc ttcgtacttc ttttgcagtt tcgcgaatgc tagctcgagc tcttcgtagc
     4861 tcgttggttc gttgttgatt gtaggctcgt tctcgtctgt ccagctcatg taaaatcgtc
     4921 tccttttcgt aatcctttcc tagtttgttg gatcgtactt ttttcttttt gtggatcaat
     4981 tcgtaagtaa aatttttccc acgattcaag caatgaatgc cgaatgtttt aagagaatca
     5041 caaaattggt tatacgttcg agtttttttg tttttcattg tttgatcaat cgcaaaccga
     5101 atttcgtctt tccaaacagg ttcatttttt tcttttaatt tgatttcaga catggttcgt
     5161 ttttcgggtt tcttccctcg ttcagtgatt gttaagtcat gttcaagaca aatctcgtca
     5221 tttaggtcat aacatttgtt taaaaatgct tgaccatggg cttgaaattt gttcccagtt
     5281 tccagattga cggagttaat gacaatgtgg ttatgaatat ggtctttgtc tgtatgtgtg
     5341 taaatcgcga tttggtggtc aggggctatt tttttcgcat actctgcccc tagttgattg
     5401 gctttttcag cggtaatttc tcctggtcga aaagactgga tcaataagtg tgcttgtaca
     5461 cgatcgtctt ttccatacag catgcgaatt tgtttcattt cgcttttggc ataatcaatg
     5521 tcacagttta tccctgtttt gacggttgct cgtggttcgg cgtaattgat cgctcggcta
     5581 caagaagtgg ttcggcttac tttaactgtt gccatatctt ttgtagctcc ttttgcatga
     5641 tttttaggtt ttcggataag cgttgggcgt cgccaggatc aacgtcgtgt tggtgtgtgt
     5701 tgcaccactt ggcaatttga ttgatattcc cgccagcttt ggctaaatcg cgcgcgattg
     5761 cctgagcaat gtctggcgcc actttcggtg tcaccagacg cgcgttttgt gccttggctt
     5821 tcacgaaagc cggcacactc atttgcaaag cttcagctga ctgcttcagc tttgcaaatt
     5881 cggattcgct cactcgaaaa ctgatttgtt ttggctcgga tcgataggta tattttttag
     5941 aatgattgct agccaaa
//
LOCUS       DAZKNH010000119         5894 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000119 DAZKNH010000000
VERSION     DAZKNH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.120"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..740)
                     /locus_tag="ACG8MG_002420"
     CDS             complement(93..740)
                     /locus_tag="ACG8MG_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0376423.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(748..1434)
                     /locus_tag="ACG8MG_002421"
     CDS             complement(748..1434)
                     /locus_tag="ACG8MG_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0376424.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(1440..2513)
                     /locus_tag="ACG8MG_002422"
     CDS             complement(1440..2513)
                     /locus_tag="ACG8MG_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0376425.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            2735..3409
                     /locus_tag="ACG8MG_002423"
     CDS             2735..3409
                     /locus_tag="ACG8MG_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0376426.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            3414..4754
                     /locus_tag="ACG8MG_002424"
     CDS             3414..4754
                     /locus_tag="ACG8MG_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0376427.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(4903..5889)
                     /locus_tag="ACG8MG_002425"
     CDS             complement(4903..5889)
                     /locus_tag="ACG8MG_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFQ0376428.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         1784 a         1129 c         1040 g         1941 t
ORIGIN      
        1 gtgttagtag aaaatcgctt tcgattttcc accaacacaa aatattatag aaccataaaa
       61 atttcaggac aaatacacat agctgtttct ttttattgta ttccttcagc aatccaacga
      121 gatttatagc gaaagtcttt taacaactct tcttttgtcc aattatttgc aatgagatcc
      181 gtcaaagtgt tcattacgag ggtcatggaa aagtatagga aactaactgc ttcttccgac
      241 gattgaatgg agaaatgatt gattcttaat aattccagcc atttttcgtt cattggagaa
      301 tcagccgata gttctctacg tttggttgtc tgctgatcat ttcgctgggt taacaagtat
      361 aacctttgcc aatagatact ttcgtgatct aagtcgcagc accactctaa atatttttcg
      421 atccccaaaa agaaatgttg tttgttttcg ctaatgaatt ttaagatatc ttgatggact
      481 atgtgagaac tgcttttgat cgtatacaaa taagcatctt ccaaatcttg aaaatattta
      541 taaaaagcac cgcgcgacat ccccatcttg tcaacgattt ctgagacttt gacttggctg
      601 atgtgctgtg agcaaaatat ctccagtaat aggtcatcga ttctctgttt ctttgcgttc
      661 gataaattga aaaaagtttc ttttggcatc cacatctctc ctttatctct gattatacgt
      721 ggatagccct tcattgtcat gaaggattca ttccttcttt ttctcttctt ctgtcaagta
      781 cccatcttcc attcgataaa cgcgatcact ccattgaatc atacggacat catgagtaac
      841 cataatagtc gctttttgtt tctcatgagc ttctttaact agtaatttga cgacttcata
      901 tgcatgatct gtatccaaac tagctgttgg ttcgtctgct aagatcaagc ttggctcatt
      961 atataaagcg cgagcaattg ccactctctg tctttctcca ccagaaaggt ctttaggata
     1021 actattttta agtgactgga tatctaaaga ttgcagcaag gaatcgattt ttgttgaatc
     1081 ttcttttttt gaagtaactt ggtcaactag ttcaaattgt tcctttaccg ttaaaaaagg
     1141 aatcaaatta gagctttgga gaataaatcc tatttcttta aagcgtaaat ctgctcgttt
     1201 tttttctgga agctcagtta gtgagtgacc gttgatcact atttctccgg aagtcggtgt
     1261 ttgcaatcct cctgaaatgg taaggaaagt acttttacct gaaccagaag gaccaatgat
     1321 gctgacgaat tctccttcct ttacataaaa atcaattgct ttcaaagctg agacctttgt
     1381 atgaccagaa ccaaatgttt tggtaacttt tttcatcgtt aatatttttt tcatttttat
     1441 taacctccta ttgctgtaat tgggtctact ttggttatcg tacgtatcga aaacaatccg
     1501 cctagcattg cgacaatgat caaaatccca ctatatagca accattgtga ccaaaaaaca
     1561 gcaaaaggca tcgcgcttgg taaaatcaga ctagtcaaat aagcaaacaa aagagcaagc
     1621 agaacgccta ccaccccaac aataaaggac tgtgctacta aagatttagc gatgaagaaa
     1681 tttttgatcc cttgtgcttt cattactcca aaaatagcag tcttctgaag tgtcatcacg
     1741 tacatgaaaa taccgaccac ggcagcaacc actaagaaca aaaagtatat catcgcattt
     1801 aaggtcatat tttgggcaga atagccagga atactttcaa tgaatgtccc aatagttaat
     1861 aactgtagac ccttcgatgc tttttctttc gaaattttag ctgaatcctt tattacgatc
     1921 ccattgatcg gctgttcttc ttcagaagca aacggctgtt gtccatactt caaagcagtc
     1981 cacgtatcta gggacgcata gatgacggga gaaactgtgt aataagtttc aggaaagata
     2041 ccgacaatcg tcaaaggatc gtcgtaactg ccaattttga tcgtatctcc aattttatag
     2101 cctgaatcag ccagattttg agagatgatg atttcattct ttgttttaaa ttcatgccct
     2161 ttcgtgagtt ttggtaataa aaatgcttct ttcgttgtgc cgaaaactgt aacattttct
     2221 ttgttttttc ctttgattgc tccgctgtac aatccgattg gtgctttctc tcctccttca
     2281 atgtatttca gatctcctct tgttaattgt gaagctgcaa atacttgatt tgcatcctcc
     2341 gacaaaacaa tctcttgtgc gtcccaatca tcgattgctt ttttaaactc ttctgaaagt
     2401 ccattcgcta gtcctgacaa catgaatacg acatacgcaa tcaagaacat aataccgacg
     2461 atcaagccat atcgtaattt agaatagcga atttctttga tagctagaaa catttgtttt
     2521 tcctccctat gatagattga tagtgacaaa atgtcacttg ttgaatagtg taactctaaa
     2581 gtgacaattt gtcacttgtg ttgttttttt cttatcaata aattgtctaa gacatctttc
     2641 gataaaacct taacatgagt cataaaaaat ttatttaagc cttatttatg ctttccatga
     2701 taaactaaag tgaccaaatc aatgaggtgg gaaaatgatt aaactattag tagtagaaga
     2761 cgataaagta ttatccaata atataaaaga aatactcgct gcaatcggcg agataacaca
     2821 agtttatgat ggtagtgaag gactgttcga gttggaaagt ggagtttatg atcttgcaat
     2881 cttggatttg atgatgccgg aaatgaatgg atatgaagtg ttagcaaaaa tacggaaaga
     2941 aaaaatacag actccagtat tgattttgac tgcaaaagat agtttggaag ataagattga
     3001 aggatttgaa cgaggtgcag atgattatct aacaaaacct ttctatcgag aagaactgat
     3061 catgcgcgtg aaagcattat tgaaacgctc gctaggtctc tttgatgaaa atgttctgaa
     3121 gtataaggat atcagttgtg atttaaaaag caatgaagtc ttgtataaag aacaagtttt
     3181 gcccatacaa ggaaaagagt ttgaattgct cgtttatttt ttacaaaata agggaatcat
     3241 tttgacaaaa gagcagatat ttgaccggat ctggggattt gattctgata cgacgattac
     3301 agttattgaa gtttatatga gcaacttacg aaaacatctg cgaagtactg gattggataa
     3361 agaaatcaaa accttacgta atgtcggtta tatactgcaa agggtgtgat gcaatgaatg
     3421 gagatattac acgaaagcaa cgtagtcgct tttttgcgac aaatgtagca gcatttgctt
     3481 ttatcttttt tcttttggga ttcattactt tacagatatt aaaccaatct gcatacagag
     3541 aaacagatct gtcattagag aaaataaatt ttgattctcg aacagtacaa ttagagatac
     3601 aaagatatca gcaaggcgat ccctttttaa acccgactca aacggaacaa aacagaacaa
     3661 acatggaaca gccattaatg gaaccatcaa atgaccgttt taacactcaa gtgatcttgt
     3721 ggtctaaaaa tggtgagata ttgaataaag cagctattgg tgggcgtttc aaccaaatca
     3781 aagaattaaa aatggatacg aaaaatctga ataccatcca aaatattgaa ttaaatgaag
     3841 acagtggctc atctgccctc tctttccgtt cgattacgaa aaaagcggag acgaatcaag
     3901 atggcgttgc ctacatccaa attttagaaa atacaaatca aatcaagaat tcattagcga
     3961 cttttcgcac gattatgatt ttgtgtatga ttgttttttg gattctctct attggtatca
     4021 gttattactt gtctagtcta agcatgcgcc ctattattct ttcttggaga agacaaaaag
     4081 aatttgttga gaatgcttct catgaattgc gcacaccgtt aacgattatt caaaatagtt
     4141 tggaacatct ttttaccaag cctaatcata ctattattga agaatccgaa agcatcgctc
     4201 aagcattgtc agagacgagg cgcctcactg gattgacttc ggatttgctg acaatcgctc
     4261 ggaatgattc tgatcagcag ctgctttcaa agcaaatgat caatacacaa gaatatatcg
     4321 aacaacttgt taaaccattt caagagatgg ccatcataga tggaaaagag tttattctcc
     4381 ataacgatgc aacattagaa gtaccaattg atgaaaaaaa aatccatcaa gttttggtta
     4441 ttttgctgga taatgcatta aaatatacga atccagaaga taagatcatt ttggattcat
     4501 ctgttaacca taaagaatgg ctgattcacg taaagaatac tggcccttct atcagtgaag
     4561 aagatcaagc aaggattttt gatcgatttt atcgggaaga tcaatctcgg gcgaaagaga
     4621 ctggcggata cgggcttggc ttggcaattg caaaacaggt aatcgaacag catcatggac
     4681 agatttctgt atccaattat cttccacaag gagtcgattt taaagtccaa ttgccgttga
     4741 agcctaaaaa ataatgctta tccctaaaaa agggctgtga caaaaatctt gtcacagccc
     4801 ttttttgttg cgaataaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt
     4861 ctttaagctg accacttctg tcacaacctc tttagcttac gcttacgatt ttttattgcg
     4921 gtcttcaatg tgtacatcac cgcttgtgtc aatatccaat tcttctcggc taacatcttc
     4981 tgtcactttt tcaacatctt catgttcttc tttatgaatc ccaacttctt cacgaataac
     5041 tgtatgtttg cttacatcta cttgctcttc tttgattggg atagtaatcg tgtcatcttc
     5101 caatttttcg tctgttccct gtgaagattg atctgttact ggtttgcgtt cgatgacgat
     5161 ttcttcacgt ttgaccggta cttctaccgt ttcagtatcg ttgaccacat gtttgtggat
     5221 gtcgacttct ccagttgtca catcatgtgt atttacatct aatttttctt cttttagttg
     5281 aattgtatca tcatctagat ttggtttgcg atcgatgtct tcagtcttgc ttgtacccat
     5341 tgtatcagtc tcgccaggta cgtccatcgg atcagttcct ggtgtcatat tcaatggatc
     5401 cgttgtattt tgaaagcctg tcgtgccacc tacgtccatt ggatctgttc cagtgccagt
     5461 ttctaatcca gtccccattg tgcttgctgg gctaacagta tcttgtgtat ccattgtatc
     5521 ttctgttgtt tctggctgac gatagccttt tactgtgatg acaaattttc catcagaaat
     5581 atctcctcta tattgatata aaggatcatt ttctggtgta gccgtctcag aatcataggt
     5641 atcaaaagaa aaagtatctt ttattttttc ccataaagaa cggtcttctt caccatccat
     5701 ccgattttca tctgtttcaa catctatacc agacaatccg tcaaatccta gtctagatgc
     5761 tgcttcatca gtagtataaa taacgatatc atctcgttca tacccttcgt tacgcaaccg
     5821 ttcaattact gataatgctt cttccctcgt actatagctt ccgatgaccg ttaaattttt
     5881 ctcttccatg taaa
//
LOCUS       DAZKNH010000120         5881 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000120 DAZKNH010000000
VERSION     DAZKNH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.121"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..565)
                     /locus_tag="ACG8MG_002426"
     CDS             complement(107..565)
                     /locus_tag="ACG8MG_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0376429.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(578..1909)
                     /locus_tag="ACG8MG_002427"
     CDS             complement(578..1909)
                     /locus_tag="ACG8MG_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFQ0376430.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1920..3362)
                     /locus_tag="ACG8MG_002428"
     CDS             complement(1920..3362)
                     /locus_tag="ACG8MG_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0376431.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3349..4113)
                     /locus_tag="ACG8MG_002429"
     CDS             complement(3349..4113)
                     /locus_tag="ACG8MG_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFQ0376432.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4217..5092)
                     /locus_tag="ACG8MG_002430"
     CDS             complement(4217..5092)
                     /locus_tag="ACG8MG_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0376433.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5347..5431)
                     /locus_tag="ACG8MG_002431"
     tRNA            complement(5347..5431)
                     /locus_tag="ACG8MG_002431"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5395..5397),aa:Leu,seq:caa)
     gene            complement(5449..5520)
                     /locus_tag="ACG8MG_002432"
     tRNA            complement(5449..5520)
                     /locus_tag="ACG8MG_002432"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5486..5488),aa:Gln,seq:ttg)
     gene            complement(5530..5602)
                     /locus_tag="ACG8MG_002433"
     tRNA            complement(5530..5602)
                     /locus_tag="ACG8MG_002433"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5567..5569),aa:His,seq:gtg)
     gene            complement(5664..5748)
                     /locus_tag="ACG8MG_002434"
     tRNA            complement(5664..5748)
                     /locus_tag="ACG8MG_002434"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5712..5714),aa:Leu,seq:caa)
     gene            complement(5769..5839)
                     /locus_tag="ACG8MG_002435"
     tRNA            complement(5769..5839)
                     /locus_tag="ACG8MG_002435"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5805..5807),aa:Cys,seq:gca)
BASE COUNT         1798 a         1253 c          894 g         1936 t
ORIGIN      
        1 aacaaaaatc aaaatgatta aatctagtga ttgaaacata tcatttcaaa aaaattttgt
       61 aaattcaccc ataaatatca ccctatttaa cttttttcat cgtacactaa aattcaaaac
      121 aactatcttt aatctttact tcttggtttt gttttaataa atttccttca aaaatgcctg
      181 gagaaactac aatgatcacc gtatcttttt gtgcttgctc caacttcttc aaatctataa
      241 tagctaacag tgtcccttct tttacctcct ggttactttc aacccgtatc tgaaacggac
      301 ttccttcgag ttcaactgta tctaaaccca tatggatcag tatttttctg ccagattcac
      361 tttcgatcgt tatggcatgt cgggtaggaa atatattatc gattattcct ttaactggcg
      421 catggatata tccatcatga ttcgttatag cgtatccaac tcctatcatt tttgaagaaa
      481 agacctcgtc ttttactgct gttaatggaa taatttgacc actagctaca gcttttagaa
      541 tgtttcttgt tttaaagaaa tgcacttttt atcacctcta ctttaatgct ggccaatatt
      601 ttttgtttac tttgatcatt tcatctagta ttttttttgc gactctggca tttggtacgg
      661 tttgattcaa tgtaaatgct tgtaaagctt tttgataaga accttcaaag taagcatcta
      721 ctagtaactt ctctactgca acttgtgcct ccatcaatcc tttatgaaaa tcactgattg
      781 gttttcttag tgtaattggc tccacaccag ttgcaccaac atatgccggt atttcaacaa
      841 ctgcatctga ccgtagattc ggaatcgccc cttgattagg aacgattagc ataaagcgac
      901 gacgatcatc attcaacaag gaaatagcta tatcgacgat atattgtcca tgctccccaa
      961 aattaaagtt caatacttct tccacaatac cgcggcgtat tttatcagcc atttctttgg
     1021 tatttttctc tcgtccatcc atgaccatat tagcacgtgt atactctttg tcagtatgct
     1081 caacgaccat atcaggatat aagtaatact ctaagtaatt attcgggata ttacttggaa
     1141 aatttttcgt gatgactgac atcatttgaa aggttttttg ccaagtttta tctcctatat
     1201 taaaatcggc gacttgcatt tcttttgtta ttagtttttc aataatctct ggcataattt
     1261 ctcgttgcaa ttctttatca taaatcgatg tataccagcc aaagtggttt aaaccataat
     1321 acgtaacgat ccaattctta cgatcataac cgtaattcac tgcaatcgtt tcttcaattg
     1381 aaatagtcat atcacaggca ttaatcattt ttacattcgg atatttacga cgaacagctt
     1441 cagaaacgat cgactctgga tttgtataat tcaaaatcca tgctttgggg gcatagtctt
     1501 gtatatgatc gacaatttcc aataaacctt tgattgagcg catcccataa gcaaatcctc
     1561 cgagtccaca cgtttcttgt cctactaatc catgtttaag tggaatcttt tcgtcctttt
     1621 ctcgcatctc caatcctcca acacgaattt gtgagaaaac aaaatcgcat cctgtaaaag
     1681 caagctttgg atcatccgtt gcacgaatgc gaatatttga aaaacctttt cttttcagca
     1741 taaatttaac aatcaagtac atatccatat ttctttcttc atcaatatca tataaacgta
     1801 tttcagataa tggcaaccgt ttttcattgt tcaaaatggc ttgaacgatt cctggtgtat
     1861 acgtactccc gcctccagct atagtgataa tctgtttttt catcatcatc ttttcctttc
     1921 tattcttcta ataatttatt gaattctgcc ataacataag tcacttctaa gccgatgata
     1981 atttgataat ttttttcagt tggtttaatg atcccgctta tccctaattg cttgattcgc
     2041 tgttcctcga ccatggtggg atccactacc tctacccgta ggcgtgtatt acaactatag
     2101 acatccagga tattggcttt tcctccaaac gcttgtagca tttttgttgc catatattca
     2161 taatttttac ttgctaaccc taactctgtt tcttctaagg ttacttcatt gccaaactct
     2221 gccttagctt cacgtcctaa tgtctgaatg tttctcttat tgatcaaata ataaaaagta
     2281 acgtaataca aagcaaaaaa gaaaattccg attggaagaa tcaaaatagc attggtagcg
     2341 attcgaaaat tgatgagata atcaacaata catgctccga atgtaaaacc aattcgtata
     2401 ttgaataagt aacagacaat ccctgctaaa ccggcaaaaa gtgcatggat cacgtacaac
     2461 ataggagaaa gaaacatgaa actaaattca acaggctcag tgatatttgc aataaatgaa
     2521 gttcctgcac cgcttcccat gattcctttg accatctctt tattttcttt ataagctgct
     2581 ttatagatag ctagacatat agctggtata ccaaacatca ttactacaaa aaaaactgat
     2641 aaaaacagtc cagcctgagg atctccgttg ataaagcgtg tcatctctcc tctgaaaact
     2701 tccttgctag gagatgtata gcttcctaaa tcgtaataaa tatacgtatt caacacatgg
     2761 tgcagtccaa aggggatcag caaacgattc aaaaacataa atatccccac acctaatgct
     2821 cccatatcaa cgagcagttt tgcaaaatta tcaatcccaa tttggatgag cggccagata
     2881 aagcccaaaa aaacagctgc gattgtttgt agaatcatca ccatagtcaa tgggaatttt
     2941 tcgcccgcaa aataagaaaa aactactggt aatttttgtt ctttaaagcg attatacgtc
     3001 catgccgcta ctaaaccgga aataattcca ccaaaaatcc ccatattcac ttctgggtcc
     3061 ataatttcta agcctttttt caaggtagca atcgctaaaa aaccagtaag tgcaggtatt
     3121 cctttgtcct tatttttgac aaaaccgatt gcaactccta tagcaaataa gatatccaag
     3181 tttccaaaaa cggcatttcc agctgcacca attactggga tgttcagcat atcgtcagct
     3241 gataaacgca tgatcaatcc cgctaatggc atcgctgcaa ctggaagtag catcgctctt
     3301 ccaagcttct gcacatttga tttaacattc atttttattc ctcctctatt aaatatcctc
     3361 atatgttact aactcaatca aaggatcatt tttcactttt tgagcaaatg ctgaatgaat
     3421 taaaaattcg acttggtggg tacgaggata cacaaaactt gaaccattta acaattcctg
     3481 atccaaatac gctgcgtgac acatgacttc gatcgtttta tcttctctca atatcatgag
     3541 taaacttgcc aaatattctt ccacactcgt attgatttgt cgtttttttt catcagtttc
     3601 tcctacgaca tcgaaacaag gctcaaaata atcgacatga cgaacttcat cttttcgttc
     3661 aaagttgctc cgaaccggca aatcatattt ttttgctaat gtaacaacta cctcctgatt
     3721 ttcatccaaa gtatgagtat gatgatgact gtctaaatga gttggtatga tccccgcatg
     3781 ataaattttt tgaatctgag cattccattc ctgatacagt tgatctttgt caatgttaaa
     3841 tggatgctga taataagata aggatctgaa ctctccgctt tcgataagag tatcgacagt
     3901 tttgaggagt ggtttgccac acgtcaatgt taaatggact cctacaccta gcctaggcat
     3961 ttcctttctt agtttcacag catgctcgaa acccggcata tttgccatca atgtagttga
     4021 tgtcaaaatt cctcgctgat aagcatccat aatcccataa ttcacaccat ggctatagcc
     4081 aaaatcatcc gaattaaaaa tcacttttcc catttcattc tccttctttt ttctactaaa
     4141 ataggcgcaa ccaaataatc atagtccatt tactatgtgt catttggttg cgcctataga
     4201 tagttacgcc cttattttaa tagattcttg ctagatgaat tgccaaatag atgatttcat
     4261 tttcttgaat ggcaagttga aattgctgtt cgatgatatc tttaatcttt agtgctgtac
     4321 aatacgcttt gctatgttta aatgtttcag taaaaaaacc aatattttct actggcgttt
     4381 cttcctgata aatcaagcgt ttaaccagga atttgatatg aaccattaag cgatgataag
     4441 tcagtgattc tttatcataa acgtttgaat aggatttttc tagatgggac aaaatcgtgg
     4501 tcgtcatttc tactattttt aagctcttta aactattagg ctctcctagc tcagcattaa
     4561 tgatatgcat agtgatgaat cctacttcat ttgaccctaa ctcggtttga tatcgatgat
     4621 tgattcgctc gaccacttct actgctatat catattcatt tggataaagg aatctcattt
     4681 ctggcaacat aattgctgtg acatctctat cttctctttt tcgttgaata gcaaaataaa
     4741 tatgatctgt caatgtaata tagatatttg aatctaatgg agaggagatc ctagctgtta
     4801 tcagttcaat cacttcttcc gcaaaatcca taaattcgat tggaatctgc gagactatct
     4861 cactgaatcg tttgatcttt tctttagaat ctaatacaaa taatttgtca gcatcctcaa
     4921 catcaaatgg cttactcact ttagcagaaa agccaatccc tttccccatc gcaatttttt
     4981 ctattccatc gtcatcgatc aatacagcat tatgattcag ctttttcatc acatacaccg
     5041 ttaattcacc tcctctatat gataaaacaa aacaacttgc agactccttc atacatattt
     5101 ttgtctccaa acaattttta aagacaaaaa acgacctaag aaagaaccct ttcttaggtc
     5161 gtgcctaatt gaatagtagc accctacata atttacaaaa atagtttaac acataataaa
     5221 agcgttatca atatcttttt tactcgtttt atgataatac ttgtatcgaa ttgatttgga
     5281 ggtaattaca taaaaacaaa aaaaatatag caactctcta taagaaagtt gctatattga
     5341 taattatacc ggcggccggg gtcgaaccgg cacgtcctca acggacactg gattttgagt
     5401 ccagcgcgtc tgccaattcc gccacgccgg caaatttaaa aataataact ggggtagctg
     5461 gattcgaacc aacgcatgag ggagtcaaag tcccttgcct taccgcttgg ctatacccca
     5521 agaataaaag gcgaatgatg ggaatcgaac ccacgaatgc cggagccaca atccggtgcg
     5581 ttaaccactt cgccacattc gccatcataa aagatgttat ttaatttctc aataaaaaac
     5641 taagatgaag acatcttagt caataccggc ggccggggtc gaaccggcac gccctcaacg
     5701 ggcactggat tttgagtcca gcgcgtctgc caattccgcc acgccggcaa gttgatttat
     5761 tagtactaag gcggtaaccg gatttgaacc ggtgatgaag gttttgcaga cctctgcctt
     5821 accacttggc tataccgcca ttatactatt atcaatctat aaaataaaga ataaattata
     5881 g
//
LOCUS       DAZKNH010000121         5789 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000121 DAZKNH010000000
VERSION     DAZKNH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.123"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1285)
                     /locus_tag="ACG8MG_002436"
     CDS             complement(<1..1285)
                     /locus_tag="ACG8MG_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0376434.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATH"
     gene            complement(1296..1964)
                     /locus_tag="ACG8MG_002437"
     CDS             complement(1296..1964)
                     /locus_tag="ACG8MG_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0376435.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2094..2678)
                     /locus_tag="ACG8MG_002438"
     CDS             complement(2094..2678)
                     /locus_tag="ACG8MG_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0376436.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(3009..3242)
                     /locus_tag="ACG8MG_002439"
     CDS             complement(3009..3242)
                     /locus_tag="ACG8MG_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0376437.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3257..4207)
                     /locus_tag="ACG8MG_002440"
     CDS             complement(3257..4207)
                     /locus_tag="ACG8MG_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFQ0376438.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4204..5046)
                     /locus_tag="ACG8MG_002441"
     CDS             complement(4204..5046)
                     /locus_tag="ACG8MG_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFQ0376439.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(5048..5767)
                     /locus_tag="ACG8MG_002442"
     CDS             complement(5048..5767)
                     /locus_tag="ACG8MG_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0376440.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT         1948 a         1051 c          709 g         2081 t
ORIGIN      
        1 catgagtagc tataactact gcaccctttt cttttaagtc tttaataagg tcagcaatcc
       61 tttccatatt aatataatcc aacccacttg taggttcatc aaatagaaat aaattagttt
      121 ttgacaatga agctatagaa accaataatc tttgtttttc tccaccggat aaatcaaacg
      181 gtaattgatc tttcatttta taaagttcca ttttttttaa aattttttct tgctgatctt
      241 tactaactat ttcattcccc agggctaact cgctcctcac tgattctgtg aaaaattggt
      301 aatccatttc ttgcattact aaaaacggcg atgacaaatt ttttttattt tcgattatct
      361 ttcctttggt agggcgaata atcttcatga gtattcttag taaggttgtt ttccccacac
      421 cgtttttccc taccaaacca ataacatctc caactttgac atcaaaattt atgtctttta
      481 gaatattttc ataagtaatt tcttcaactt ttaatacatc tgcacttacc ttttcgatat
      541 agggaacttc aatcgaacta ttaaaaatgt caaatttcct taatgcgtta ttgttttcag
      601 tttttaacaa ctgaatagtt gtagttttta cagttctatt tttaacaaat agaacttgat
      661 caattaattc tttcaaatag taaaaacgat gttcagctat aacaatagta aatcctttat
      721 tttttaaatc acaaagtaag tcagcaagtt tcttagtcgc tttgaagtcc aaatttgaag
      781 acggttcatc caatataatt aatttagagt ttagcgataa actacatcca atagaaattt
      841 tctgccgttc accacttgat agcgaaaaaa tgtttttatc taatatattt gaaagatcta
      901 ataattcagc taattcattg attcttttat ccatatgctc ttttgtaaat ccaaagtttt
      961 ccatcgaaaa tactaactca ctcgttgtat ttgttgtaaa gaattgtcct ctaggatctt
     1021 gacttacgta gccaatattt tttgatatac tttcttgtgt taaggattgt agcgagttat
     1081 tttcaaataa aatttcccca accaattttc cgtcataaag tcttggaata agtccattca
     1141 ttaactttaa tagtgtggat ttcccactcc cagaagaacc agtaatcaat gtaagactgc
     1201 ctttttttat tgaaaaatta aggtcaacaa gcgcttgttc cttaacatcc ttatatgaaa
     1261 aagaaacatt gttaaattca aacaattctt tcacctcaaa tcaaaatact taagaaaaaa
     1321 atagtaacgg aaaacaggat gatatagtct aataagctca tgtttgtttt atagtaagaa
     1381 cttcttttca caggagattc tatccccttt gttattgcat tagcggtcat ttcttcagca
     1441 atcatagttg cacgtataag aacgggaact aacagaaatt ccaaatattg cagaggctga
     1501 aaaaaagaca agcgaatgtt tctcattttt aaagattctt taatcattct tatttcagaa
     1561 cgataagtcg gaaagaatct gatcactact gtaatactta acattatttt tttcggtaaa
     1621 tgtatttttt ctaaagagga taataattca ttgctagtat agacactaac caacaaccat
     1681 gagatcatta cgaccggcaa cattcgaaat acaatatata aggatgtcgc aaaaaatata
     1741 gtaaaaaact ctatcttgat agcatcaata attttcatta ctaaaaacag aactgaataa
     1801 atcaagatga aataaataca tgtctttatt ttacgaaaca tcaatactaa aactgtagca
     1861 ataatgaaac accccacttg aaatatattt ccctttccta aaaaaataat gaccggaaaa
     1921 aaaatattta aagtaatcaa ggttaacgga ttgatttttg acactctttc acctccaaaa
     1981 aataagatcg cactgttatc ttaagctgat aaaaatgcac cgttattcag ataacagtgc
     2041 gatcttatca aaaagcataa ctgagaacaa ttctcaatgc aagctttttt taattaactc
     2101 agtaattcaa tccacccagg aacagaaaat tgatacaatc ttctggttaa tagcatagtc
     2161 ttttcttttg aatagctatg cttcaatata tcatagaatg tagcgtagaa tgagtaaatg
     2221 tagaagtgga tttcttcttc attaaagact aaattttgat tgatcattcc gatagtcttt
     2281 aacttatcta gacaacagac tgtctttttc gtcacaaatg atgtaatttt ttcaacaata
     2341 tcttgatatt tggttccagc tgattggaat aaaatcaaat cgaatatacg tttattctca
     2401 aaaatataat taataaatgg atctaattca ttgaagttct ctctccaact tttctctata
     2461 tctatatttt ctaagtttaa gaggtatttg gtaaactctt cattatacat attaataagc
     2521 gcattaatat gcggctgaat aatttcttca aataacgctt ccttagaagt aaaatgttta
     2581 taaaaagcac ccgtagttac gcctgcggtc aggcacagtt gccttatctt agtttgctga
     2641 aatccttctt tttcaaaatg gcttagtcca ctttctatta acttttggtg agttataata
     2701 taatctctgg tttctatggg tacttcctcc taataagatg aattaacttt attatcttac
     2761 acaggacttg aagtttcaat cgttaacgtt tattactgaa ctcattcctt agagctaaac
     2821 taatgagaaa gagcagaata actgatacaa aaagcaatcc tacaagaatt aatagactca
     2881 tctaaatcat tcaccttttc taaaataatc cgagaaagat ttaaaaaaat tatatccagc
     2941 tattaggata agtcctatca taatcactcc aatagccatt ttcttatcct ccctaaaaat
     3001 aaagtctttt acttaaaacc ttttttgtaa tcgtcaataa attttttccc tttatcagtt
     3061 aattgaaatc ctatatctaa atagtcctca tatctaagtt tagccagcat ctcacttaca
     3121 gatgctggac taacacttag aacttttgct aaatcattat tttttaattt cttttttctt
     3181 tttgataatt cctgaattgc tagtaaataa attagtatag tagtcgtttt aatttgtacc
     3241 atacctaccc tccaaattat ttcgaaaggc cttcagcaat tttatccagg ttccatttca
     3301 tcatcgcata gtaactatca ccatcttgtc ctttttctgc aattgaatca gtaaaaatcg
     3361 ttgaatagat aggaatattg gtatcttttg atactgtttt cattggtcta tcatccacac
     3421 tgctttctac gaataaggag ggaacttttg ttgtgcgtaa tttttcaact aagtgtttta
     3481 tttgatctgg tgttccttct tcttcagtgt tgatttccca aatgtaagca gaaggcacat
     3541 tatacgcttt cgagaaatat ttaaagcatc cttcacttgt aacaatcatt tttttatcat
     3601 tcggaattga agcaaattta gatttagctt ctttgtctag agaatccaat ttttcaatat
     3661 acttatctag attttcttta tagaattttt tattatcagg atctttttcc gctaactgtt
     3721 tttcaatatt tttagcgtaa ataataccgt tttctaaatt taaccaagca tgaggatctt
     3781 ccttcccttt ctcactctga ccctctaagt aaataacatc tatgccatca cttgctgcaa
     3841 aatagtcttt gttttcctct ttgttcgcat ttttaactaa ttttgtaaac caagcatttc
     3901 ctccagtttc caagttaaca ccgttataaa aaatcaaatc tgcttttgaa gttttttgaa
     3961 catcttcagg caaaggttca tattcgtggg gatctttccc aataggtacg atactgtgta
     4021 aatcaatttt atcttttgct atattttcgg taatatctgc taaaatcgaa ttagtaacta
     4081 ctacttttaa tttttcgttc gaagccactt tatctttgtc tttactattt attgaacatg
     4141 ccccgaatat aaaaagagca atcaaaatcc ccagacttcc cagtactgct ttccattttc
     4201 tcatcataac gatctctcct tctttcttaa aaatttttgt tttggtgaga ttagaaaact
     4261 aattacaaat aagaatgccg ctgttaaaac aatacttgat cctgctgcta tgttaaaact
     4321 ataaccaata aacaatccaa gcaatgatga acttgctccg actgttgcag aaataaatat
     4381 catagttttc aagctatttg catataaata tgctgtagct gctggtgtaa ttaacattgc
     4441 aacaattaat atagttccca cactttgcat agctgttact gctactaaag ttaatagaac
     4501 catcaataga taatgataaa aactgacatt catccctatt gcttttgcca ttaatggatc
     4561 aaaagaagtg attaataatt ctttaaaaaa tagaactact ataaccaaca ctaaaaccga
     4621 aacgccaatt gtaatccact tatcaatatc ttgtacagct aaaatatttc caaataaaat
     4681 atgaaataaa tccgttgagc tatttgcaac tcctataagg ataactccta gtgctaaaaa
     4741 agaactgaaa gtaataccaa ttgctgtatc tccttttatt acactattgc tttttataaa
     4801 agtaataatg atagaagcta atagaccaaa tacaatcgca ccaataaaaa aattaatacc
     4861 taaaatataa gaaattgcta ctcctggcaa aacagcatga gaaatagcat cacccataag
     4921 agacattcct cttaaaataa taaaacaacc tactgttcca gaaacaatcc ctataactac
     4981 agatgtaatt agagcattct gtaaaaagtg aaagtcatac agaccattaa taaaattttg
     5041 aagcatatta ttccccctct ccaataaata tagtatctcc ataggctttt tttaaatttt
     5101 cttcattaaa tacagattct acacttccgt gagcaattag atttcgattc aaaagaataa
     5161 tattatcaaa atacttcttt accttgctta aatcatgatg cacaattagc actgtttttc
     5221 cctgatgttt caattttttt aacgtatcca taattattcg ttcactaact aagtctatcc
     5281 ctacaaatgg ttcatctaaa aatatgaaat ccgcattttg agcaagacat cttgctaaaa
     5341 gtacacgttg aaattgtcct ccagataatt ctcctatttg atgattagaa tattttccca
     5401 tatcgacctt ttccaaagcc tttgaaactc tttgccattc tgcattttta attctttgaa
     5461 aaactttcat tccggcatat gttcccatag atacacattc ttttactttt attggaaaag
     5521 taaaatcaat atcagctttt tgctctacat atgcaatctt caccagtttt ttattgatat
     5581 cttcattatt aatggagacc tctccctgat gagggataat atttaacatt gctttaagca
     5641 atgtggattt tccagcacca tttggaccaa taatacctgt taaagcacct tcttgtattt
     5701 ttaaagaaat ctcgttcaat gctaaaaaat cgttataaca aacattaagc tttttcactt
     5761 ctaacataac tttctccttc taaatgata
//
LOCUS       DAZKNH010000122         5749 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000122 DAZKNH010000000
VERSION     DAZKNH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.124"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1128)
                     /locus_tag="ACG8MG_002443"
     CDS             complement(<1..1128)
                     /locus_tag="ACG8MG_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0376441.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
     gene            complement(1164..1874)
                     /locus_tag="ACG8MG_002444"
     CDS             complement(1164..1874)
                     /locus_tag="ACG8MG_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0376442.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            complement(1942..2802)
                     /locus_tag="ACG8MG_002445"
     CDS             complement(1942..2802)
                     /locus_tag="ACG8MG_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFQ0376443.1"
                     /translation="MFVAIWGKWSTRRIKMKVLGLGDNVVDKYENLGIMYPGGNSLNF
                     AVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGD
                     RIFLGSNSGGVTREYPIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYD
                     FSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIG
                     YDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVD
                     GSFGFGIHYD"
     gene            complement(2768..3736)
                     /locus_tag="ACG8MG_002446"
     CDS             complement(2768..3736)
                     /locus_tag="ACG8MG_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0376444.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            4499..>5749
                     /locus_tag="ACG8MG_002447"
     CDS             4499..>5749
                     /locus_tag="ACG8MG_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0376445.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT         1772 a         1183 c          915 g         1879 t
ORIGIN      
        1 attttttata acagttgaca tgtaagcctc ttctaacgtt tgattcaagc cgagaatttg
       61 tggatatacc tctgctacta cttgtccttc gttcattttt tctgctgtct tttgagtaac
      121 catttccaaa tcactaatcg aattaaaaac gccttcaggt aaccgctttc caaattcgac
      181 aagaattgac gcagctttat aacccgcttt atctccttcc gttgtcaaac agcaaggaat
      241 ctctacttca ttgaatgcag caacttctgc tttaattgcc tcatagtcaa tctcgggtga
      301 agctactcct gactgataaa caccgtaaat atctccactt ctatccgcaa caacaccatc
      361 taccatgatc ttcactccac caaagcggac cttttcaggg aaaacttttt ccttatactc
      421 atatagaagt tcatgctgta gaggttcttg gactgcctgc acatataggt tactaagtat
      481 tttcctctga ggtatattag aaagaaattc tgcgtcatca aaaacgattt ctttcattga
      541 tataacacct tttgaaagca gtaatgcttc aaattcagcg taaacctctg ctaaaagctt
      601 tttgtttgct agcatttcct taattaataa tactcgactt tcagcactgg tttctttttc
      661 tgtaaaacca taagtctgct tagcaatagc attcatccaa caatggctgc gatttttgtc
      721 gatcgctgtg attggaccaa agtggtttat ttcattcaat agctcttctg atgggtcagt
      781 tttccacagg gcagcatccc agccatgagc ataaattggt tcatctgctt ttttatggtt
      841 cattacagct ttaatacgct ctacagcctc gttagctgaa gatgtatcgc ttaaatctat
      901 gccagcattc atagccaaat aaccactgaa aaatacatga ttatcatgaa ttgctggcat
      961 aacgaattga tcatcataag caattaattc cgtgttttca tttaatagat cactgtaatt
     1021 ccagttgttt tccacctttt caatgatccc ttcattgatt acaatataac ctttgaaagg
     1081 tggctgctct ccatcaaaaa tcatttttga ccgcaatatt tgcttcataa ataaaatcta
     1141 ctccttgaaa tccaaacagg aaatcataag taaaaacgat atacttctcc caggtaatat
     1201 tgattaccta catgaattgg tatgttgttt tgatcgttga ttaaagtatc cataaagaaa
     1261 aagggcgtcc caacagctac ttccattatt ttggcaattt ggtcatcagc tgtgacaatt
     1321 tcaagtgcag tttccccagg attgatggct aaggtacctt ggactttaag tacttcatac
     1381 aaagaatcag tcagctcttc ctccattaag aaactatatc tttcataaga aaaataattg
     1441 ttttctaaca taatggggat gttatccgcc attcgctttc tctggatata taaaacatca
     1501 tcaccttgag cgatatcgag tttttttgcc atcccttcag ttgctggaat gatcttgcgt
     1561 tccaaaacaa tgctagtcgg tgtaaaacca ttagatttac aactctcact gaaacccata
     1621 atatattcaa ttttacgaaa aactttatgc cggttgacaa aagttccttt cccttgtttt
     1681 ttaagtaggt acccttcttc taccagttct tctactgctt ttctcactgt gattcggctg
     1741 acctcataac tttctatcag ctctgattca gcgggaattt tcgtattctc gggatactcc
     1801 cctgtattga ttttattttt taatatttct tttaattgta agtacaatgg taattgtgca
     1861 tttgtatcca gcattttttt aacctccact ttaccgggat ttctttcttt aggttatcaa
     1921 atgcaggaat ttaaaacaag ctcaatcgta atgaatacca aagccgaacg aaccgtcaac
     1981 cagacaagac ttcccagcaa attcattccc ctttttcatc gcggtgacta tatcgtcccc
     2041 ttcgagaaaa taaagtagga aagctgttaa gaaagaatct cccgctgcca ttgtatcaac
     2101 tggttcttct agcacggggt tcacttcata aaactttgct ccatcataac caatagccgt
     2161 tttttctcct cgggttgcaa tcaatacatc atttcgctca caaaaatgct tcatcaaaaa
     2221 ctgctttact tcttcatcgg aaaagtcttc tatagaaaag aagccaaaat caactttatc
     2281 aattgcccgc tgaacctctt cttccgtaaa gtcgtcagaa aaatcataag aaatctttcc
     2341 agaaacattt tgtaactctt ctaatagatg attgacatgg ctatataatc ccatatgaat
     2401 caattcaaac tggtttaaat attctctgtc ttcttcagta agctgaatcg ggtattcacg
     2461 agtaacacct cccgaattgc tgccaaggaa aatccggtcg ccctcatcaa tcttcactct
     2521 ggcacagcca ttctcgcctt tttcaaagcg agagtgactg ttgtccagtc ctatttcttt
     2581 tattgctgat aaaacatgag ctgcctcctc atctgagcca aaaactccca taaagctact
     2641 ttcatgtccc agtttttttg caaaaactgc aaaattcaat gaatttcctc caggatacat
     2701 aattcccaga ttttcatatt tatcgacgac gttatcacct aagcctaata ccttcatttt
     2761 aatcctccta gtactccact ttccccatat agcgacgaac atctaaatca tgattgcgta
     2821 catctgctaa agctgaacga tattctgaaa gaatactgta gaataaaatg ggattgaaga
     2881 attcagaaac ttcatctgat atcatatcca atcctagttc ctttgcatca acgatttcta
     2941 ctctttctgc atatttatca aggaatcgtt ttgcccgttc atctaaagca cgagtgcgtc
     3001 caatgctcat caagagaata aaattcgtat ctttgtctgt tacttcaaaa ggtccatgga
     3061 agaattctcc agaatgaaca gaagttgaat caagccattg catttccatc aaagagcaaa
     3121 ttgaaaaacc ataagcatgt ccatatgatg gtccactacc taagatataa aacattgttt
     3181 catctttgta cttatcagca aacactttaa tacgatcctg cacctgctta atgccatttt
     3241 caattacggt atcgataacc ttcatgcctt ctctgaattt accgtaatgt tcaaatgttt
     3301 cggtaacatt gaccaaagat acagttaaat caagaatgat ggccatagga ttattgacta
     3361 tttttgcatc atctccccat tcgtaaagga aactatgatc agaaaccttt aataaatccg
     3421 cagttttatt atgagtcaga ccaacagtca cagctcctct agcttgagcg acttctcctg
     3481 cttttaccgt ttctgctgta tttcctccat gagaacatag aatgacaaca gatttatcat
     3541 ctaattgctt tggggttaca tggttaaatt cgttcgctgt atataagcct gtaatacact
     3601 tcgatgcttc ggctttaaga aaataatctg agacatacat atctaccagc gaaccgccac
     3661 aagccaccaa ataaattcca gaaatctctc catcattttt ttctacaatt gcgttaaccg
     3721 tttttgctac atccatcaac atttcctcct taaaatttta aaaatgttaa ttttatccaa
     3781 aataaaagta actgattaaa tgtaaagata tcacataacg tcatataacg tcatgtgaca
     3841 taattgcatt gtatccgctt tctttgtgtt tgtcaatagg aaaaatttat ttctctaaaa
     3901 aatataaata atctgcagtg aaacttgagt acatatacta atttcgaaaa atgcctatga
     3961 tttaaataat tatggtgctg ggatgaaggt ttataagttt gtcttgattt gtttagaaga
     4021 aatcgttttt gcacacagtt gaattaggaa actactggag tggaaaataa agtgatttga
     4081 cgggtagatg ggtgcactta aatttgaaaa tggcttaaaa tgctttattt tgaattgcag
     4141 aatttctttt agtacgcaat ttatggtctt aaaactgatt aaaaagtgat acattgattt
     4201 taatacaatt tatgatacaa aaaatgacct cctttcttaa ataaaagagg tcattttgtt
     4261 ttgtctcata atcgattgtt tttgatacag tagttatatt cagaaaaaat aaagactctt
     4321 ctgagtttat aatcctttgg attatatacg gatacagatg tagacataaa acaagctcaa
     4381 ttttcaaaaa tggcattact cacctttttg gtgaattatt tgtataaaaa aaatatagaa
     4441 aagacaccct gaaatccttt agaattaatt tggctaccaa ttcataggag gattcaagat
     4501 gtctcatact cagcttatca aagataccct aaatattcta gacctaaata tctatttcga
     4561 ggaaaattgt ttaactaaag aaaaatacaa agggcaaatt tgcttgattt acaaaggaac
     4621 tcttctgtac aagccagaag agtgttccca ttgtctttgt gtcgtcccct ctaggatcat
     4681 tcgttggggg acgacaaccg ttcggttgtt actgaatgat gtgtctgagt atcgcactta
     4741 tctggagctg aagaaacaac gatttaaatg taagtcttgc caaagaacat tcgtagcgga
     4801 tacctctgtt gctgaaaaac actgtttcat tagtcaaaaa gttcgttggt ctgtcattgc
     4861 tcgcttaaaa gagaatactt ccatgacgga aatcgctcgt cagaaaaata tttccacttc
     4921 ttcggtctat cgtgtcatga agcgattcta ccgcccattg aatcctttca aacagacttt
     4981 gccaaaggtc ttatgctttg atgaattcaa atcggttcga agtgttgcca gtgcaatgag
     5041 ttttatcatg atggacggac aaagccatgc attattggat attgtcgaaa atcgacgctt
     5101 gccttattta gaacgctatt tttctcggtt ttcattagcg actcgtgaag ccgttcaatt
     5161 gattgttatt gatatgtatg cgccttatgt ttcgttggtg aaaaagcttt tcccgaatgc
     5221 tcagttgatc atcgatcgtt tccacatcgt ccaacatatt ggcagaactt tcttgaatca
     5281 tcgagtgaaa gaaaccacgg tacgacttaa aggaaattcc cactctcaac gagggttagg
     5341 gaaaaaattg aagcgctatt ggaaactcct acaaaaagaa gaagcaaagt taaactatga
     5401 aaaacgggtt tggcgtgcca gttttaaaga ttatttgact gaaagtgaga ttgccgatcg
     5461 actgttagct gcttgtccca acttacgcca aggctatcag ttataccaag acattcttta
     5521 tgcggtaaag aaaagagatc agtcactatt tgaggattgt ttaactagag acattagcgg
     5581 tctgccagaa acctatacaa ccactttacg aacgtttaaa aagtttctgc cacagatcca
     5641 aaatgcgctt cactacagct attcaaacgg accattggaa tgcttaaaca accacatcaa
     5701 agtattaaaa cgcaatgcgt acggctttcg caacttttac aatttcaaa
//
LOCUS       DAZKNH010000123         5689 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000123 DAZKNH010000000
VERSION     DAZKNH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.125"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(488..1297)
                     /locus_tag="ACG8MG_002448"
     CDS             complement(488..1297)
                     /locus_tag="ACG8MG_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0376446.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACG8MG_002449"
     CDS             complement(1297..2043)
                     /locus_tag="ACG8MG_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0376447.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACG8MG_002450"
     CDS             complement(2061..2528)
                     /locus_tag="ACG8MG_002450"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0376448.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACG8MG_002451"
     CDS             complement(2528..2938)
                     /locus_tag="ACG8MG_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0376449.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACG8MG_002452"
     CDS             complement(2949..3962)
                     /locus_tag="ACG8MG_002452"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0376450.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACG8MG_002453"
     CDS             4179..4913
                     /locus_tag="ACG8MG_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0376451.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ACG8MG_002454"
     CDS             4897..5607
                     /locus_tag="ACG8MG_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0376452.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAZKNH010000124         5601 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000124 DAZKNH010000000
VERSION     DAZKNH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.126"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..2217
                     /locus_tag="ACG8MG_002455"
     CDS             157..2217
                     /locus_tag="ACG8MG_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFQ0376453.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2300..2869
                     /locus_tag="ACG8MG_002456"
     CDS             2300..2869
                     /locus_tag="ACG8MG_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0376454.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3044..3850)
                     /locus_tag="ACG8MG_002457"
     CDS             complement(3044..3850)
                     /locus_tag="ACG8MG_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376455.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3988..5502
                     /locus_tag="ACG8MG_002458"
     CDS             3988..5502
                     /locus_tag="ACG8MG_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFQ0376456.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         1994 a          968 c         1130 g         1509 t
ORIGIN      
        1 acataagtta ttattccgat tttcttgaaa caataaaaaa tcaaaaaaaa tactctccta
       61 atagtccatc ttcttttgat tttatttata taatgaaatc atcttatgaa aattactact
      121 tcatgtttag acaagagtag ggaggactaa gaataaatgt gttcaaaaag acgttcaaaa
      181 caactaaata aacacgcttg tcgtttagat aaggaacggt cgtgttctga aaaacactac
      241 gttaagttta tgatgcgcac gatgctgatc agtttatctg taggtgtcta tatgcatgta
      301 cccgttcagg caacgacaca aaccatcaag gtcgtacgta cctctgtttc tgagaccact
      361 gattccccta tcactattac tcatactttc actgatgcaa atggaaaaca gctacttcct
      421 gcattgactt cctataataa agtcagttat cctccaacga taaaccacta ctggttaatt
      481 cctaacaaaa cattatggac atcggaaacc ggaagcagtg tgccgctttc tatcttttcc
      541 gatatgactg atcctacaaa aggtgatcag ttaaaacaga tcactgatat tttgaatcat
      601 gctttgacag atataaagga cggtggaagc gcacaagttt cttatgtata tgaaaaagat
      661 tattcacagt ttgaaagaaa gaaaatcgat gttctggcag ggacaaaagt ttcgatatct
      721 gatctggtag aaaggctgaa gaacgcagat ggaacacaag gagatatgtc caaagtaatt
      781 tcaaacaatg ggaatatcgt ggatacgact aatacaggaa catctactat ttccttgtcc
      841 tattttgatc caattgcttt aaaaacaatg actgcatctg ctgtgatcca tgttcgtgct
      901 gatcaaacag ctatcaaagg aaaagatctt cacttaagaa caggagagag ttacgcccct
      961 aaagatcttg ttgaatatgt aacaaaaagc gatggaactt caggaaataa agaagaaatc
     1021 aaaatgaacg ggacagtaga tactacaact ccgggagaat atcccgttgt attaacttat
     1081 atagactcga aaacatcaaa tgtggcagaa acaacagctg tagtttatgt agaagcatct
     1141 gctgcttcat cgactgtcat cactcatttt atagatagtc aaacaaataa agaacttcaa
     1201 gcagaaacac atatgagtag tgtaaatcgt attcctgatt tgcgcctacc tgctgaattt
     1261 tctgatcgag tgccaaatat agatttatcg acttatgaac aaaatgggaa agtcacttct
     1321 atttctgatt ggatgcaagc aaacggatta gacagaaaaa gtcactggtc ggagatcctt
     1381 gaagtacttc actcagaact gatgaataat aggaagcaat caggagggga aattaccttt
     1441 acttatgttt atataccaga caatcgttca ttagaaggtc atgatcttat tgttcatcca
     1501 aacgaaaaaa taaacaaaga agagttgatc caatcagcaa caaattcctt aggtaaaccc
     1561 gtaccgttaa aggatgtatc ggttacaatc aatgaagtac cattggagga tggttatttg
     1621 accaaacaaa caggtgattt tacgattatg tatagttatc atgacccgta ttcactccaa
     1681 gaactccatg ctcagtcact attacatgtt ttaccattaa acaaacagat agatcaaaaa
     1741 gaacaaaaaa gacaagaaga aagcttgtca gaaaaaaaac aacaaatggc tcatgcagtt
     1801 tctccaacag ggaaagtaaa acaaacaaga agcctagagt tagcaagaat atctagaaaa
     1861 atctcagaat ctaataaaac gataaggcaa gaagctcatt tggccccaca agtttctgga
     1921 ttggaaaatg aaattttaga gcctaaaata aaacaacaat cagaaaaaga atttgttgct
     1981 aaacagtcgg aagatcagaa ggaatcagat gaccagaagg aaacgaagaa aaaacgcttg
     2041 tcaactattg attccaccga tgagcatctg cagccgcaag taccacttcc tagtccggga
     2101 acacccgcac ctggtggagg tattccttct tctgctttag gagatttcat gcgaggtatg
     2161 gcaggaacat gggtatacgc taatcgaaat gaatttgacg cagatgactg gttctgattt
     2221 atatctctat ctgttatagt caaataagta gctgagtcat atgttatttg gcataatgga
     2281 gacgaggagg gatagaaaaa tgttagataa aacgttacct tacgcagaaa tctggatgac
     2341 aagaccactt caacaaaatg ttccaatcac gcctttagcg gaaggttttg aatttcgatt
     2401 ttatcaaaaa gaagatgaat ctgcttgggc ggagattgag acttccgttg gggaatttca
     2461 aacgacagat gaagcacttg agtattttca acaaaacttt gctccttatc ctgaagaact
     2521 tgaaagaagg atgttttttg tagagacaaa aaaaggagaa aaagtagcaa cctgtaccgc
     2581 ttggcgaaaa aaatcaagag agcaagtgta tccggttttt cattggttag cagtgaaacc
     2641 agattaccaa ggaaaagggc tcgcaaaagc attgactgcc agagtcttgc aagaatttcc
     2701 taccctccat acggacggac caatctatct tcacacacag acatggagcc atcaggcgat
     2761 tgctctttat aaaaaaatgg gttttacttt tattgctgaa aatctagacg gcacacctaa
     2821 tccagattat gaaaaagtta tgcaggtttt aagtaaatta gataaataac gaaatggtga
     2881 aataagtcct cagtattggg ataaaaaacg aaatagtcat tcctcatatc ttactttttt
     2941 gagttaatac tatcggataa ctaatggcat gccatacatt caaaaaagaa ggggcagtga
     3001 caaaagtatt gtcactgccc cttctcttgt tttttagcat gttttatcgt ttgatttttt
     3061 taggaataaa attcttgatg aacgtaaatt tttggtaatc gtttcgacta tagaggtaac
     3121 caaatacact gaaaacaact gctccaataa aattaacaaa taaatccttc atagtatcca
     3181 tgattcccac gtctaagtaa ccattttgga tgattgtttt atctgtttta ccatctgcat
     3241 tttgactttc aatgattgtc cgagtgatat ctggaacatg atagacagtg tttccgtctt
     3301 tacttaactc cacactattg atttgctgga tgatcgtatc tttttgcata tcgaattgca
     3361 gtaatttatc tgctgaaaat tccacaaact cccacataac accaactgtc atcgaaaaac
     3421 agaaggaaac aactgctagt aataaaggag tcaagttcat ggtttttgta tttttattga
     3481 gcaaataaac aagggaaaag ccgacacccc cagctaaaaa tccgctgaat gcatgtagaa
     3541 aagtatccca cattgaaaaa tagccataaa aatcacttaa ctctcctaaa actgttgaac
     3601 taaaaataaa agataacaaa atgatttcta gcatatttgg aatctgtatc tttaatatct
     3661 tttccagaaa ataagggatt tcaaataaaa ttaatgtcag taataaaagc attgtatttc
     3721 cccagtgttg gttccagacc tgatccacta gcataataac gacgatgata cgtaataaaa
     3781 tatagattcc gtttgtgatt ctacctgcag tttttgtact gttcgtaaaa aaggttttca
     3841 aatgattcat aaaataactc cttcatgtat atccttaatt atactaagaa gcgtgtgttt
     3901 accaaagcta tatgacttaa agaaaaacag agttgatatt gtgaaaaaaa tcacttaata
     3961 tagtataagg agaaaagggg gatacaaatg aaaaaattag ttgctggttt attaacagcc
     4021 gttgcacttc ttgctatgag cgcatgcggc gcagatcaga aaaatacagc gcagacaagt
     4081 tctagtacga aggaatctgc cgaagtatca tcaggcgctt ctgaacaagt atatacagat
     4141 ccaaaagaaa tgaaagaatc ttatgatgtc gtgattattg gagctggtgg tgcagggatg
     4201 gcagcagcta tcgaagcaaa agatgctggg ttgaatccag taattttgga aaagatgcct
     4261 gttgccggag gaaacacatc taaatcatca agcggtatga atgcttcaga aacaaaattt
     4321 caaaaagaag aaggaatcaa agactcgaat gatgccttct atgaagaaac gctaaaaggt
     4381 ggtggaggaa cgaatgataa agaaatgctt cgatactttg tcgatcactc tgctgatgcg
     4441 gtcgattggc tagatcaaaa cggtatcaaa ctggacaact taacgatcac tggagggatg
     4501 agtgaaaaga ggacacatcg tccgtctgat ggctcggcta ttggtggata cttagtggag
     4561 gggttattga ataacgtcca tgagcgggaa atccctattt ttgtcaacgc agatgtgaca
     4621 gacattaaga aaaatgggga aacggtcaat gaagtgactg tacatgttca aggagaagaa
     4681 ccaaaagagg tcaccggaaa agcaatcgta gttaccacag gcggttttgg tgccagcaaa
     4741 gaatttattg aaaaatatcg tcccgattta gctgattata ttacgacaaa tcaagaagga
     4801 tcaactggag atggtatcac catgattgaa aaattaggtg gacaaaccgt tgatatggat
     4861 aaaatacaaa ttcatccaac tgtgcagcag gatgaggggg tcttgattgg tgaggttgtt
     4921 cgcggagaag gagcaatctt ggtcgatcaa gaggggaaaa gatttgtcaa tgaaatggat
     4981 acaagggata aagtatctgc agcaattaca gcattgcctg aaaaatcagc ttatttgatt
     5041 tttgaccaag gcgtgcgtga tcgtgccaca gctattgaat tttatgacca aaaaggatat
     5101 gtcacaactg gaaagacaat ggaagaatta gcagaaaaaa tcgatttacc aaaagaaaca
     5161 ctagcaaaaa cagttgatgg atggaatacg gatgttgcgg agaaaaaaga tgctcaattc
     5221 aaccgtgcca cagccatgga acatgactta tcgtctccga attattatgc gatcaaaatc
     5281 gcaccaggaa tccattacac gatgggcggt gtgaaaatca atacgaacac tgaagtatta
     5341 gataaagaaa accagccaat tagggggtta tatgctgctg gtgaagtcac aggtggctta
     5401 catggaaaca accggatcgg tggaaattca gttgcggaga tcattatttt tgggcgacaa
     5461 gcgggcatac aagcagctcg atttgcttcg gaaaatgaat gaaatagaac gtaaataaaa
     5521 agaggttgtg aaatcagcgt tttgagctaa taccgagggt cagctgattg aacactgttt
     5581 agtgaagagg ttgtgaaaaa g
//
LOCUS       DAZKNH010000125         5542 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000125 DAZKNH010000000
VERSION     DAZKNH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.127"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..802
                     /locus_tag="ACG8MG_002459"
     CDS             116..802
                     /locus_tag="ACG8MG_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFQ0376457.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            804..1748
                     /locus_tag="ACG8MG_002460"
     CDS             804..1748
                     /locus_tag="ACG8MG_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFQ0376458.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1947..2591
                     /gene="pcp"
                     /locus_tag="ACG8MG_002461"
     CDS             1947..2591
                     /gene="pcp"
                     /locus_tag="ACG8MG_002461"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFQ0376459.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2670..3947
                     /locus_tag="ACG8MG_002462"
     CDS             2670..3947
                     /locus_tag="ACG8MG_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376460.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            4205..5422
                     /locus_tag="ACG8MG_002463"
     CDS             4205..5422
                     /locus_tag="ACG8MG_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0376461.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
BASE COUNT         1783 a         1024 c         1167 g         1568 t
ORIGIN      
        1 aaaaaataac gaaaaatatt taaaaatagt gtctatttcg tgagaaaaca actatatttt
       61 ttatgtgttt tgctttataa ttaaaaaaat attgtcattt tatggaggaa aatatatgga
      121 aaattatctt agccttttag gcgtcttgat tgttattctc gggtttgcat ttaaattaga
      181 ctcgatattg atcgtaatgg ttgctttagt tgttacagct ttgactggcg gtcttggcat
      241 tcaaggaatg ttagaacttt taggtacaag ttttgtaaac aatcgcggga tggcgatttt
      301 tatcatcatt atgttggcaa caggaacgct tgaaagaaat ggtctgaaag aatcagcggc
      361 ttccttgatc aagcgattca agaaagtatc tgctggtatg attgtagata tttatgggat
      421 ctttcgaatg atctttgcgg cttttaacgt gagttttggt ggagttgctg gatttgttcg
      481 tccaatcatt ttaccgatgg cattggggac catcgaatca aaagggttag aagtccatcc
      541 taagcatgaa gaagaactaa aaggtatggc ttcagctatg gaaaatgtct gctggttctt
      601 tggccaagta ctattcatcg gaggagcagg ggcgttgcta gtccaatcaa cactgaaaga
      661 tctaggatat gacgtaacat tgggacagct tgctttagtg gaagttccag tggctatcac
      721 ggctttagtt gtggcaagtg tctacttcta tcttaaagaa aagaaattag cgaagaaata
      781 ttatccagga aaggggcaat aagatggact tttttacaaa tcctgatatt ttattaggcg
      841 ataaattact ggagatcctt tatatcgtga tgggactcgt attgatttac actggagtaa
      901 gaaatttaac agataaaacc aatcctcatc gttatgggtc agcctttttc tggacggtct
      961 taggggtagt tattgcagga ggaagatggc ttccggcaat cgtgaacggt ggcttgatct
     1021 ttgcgatgac cataccagca attgctaaga aagtcagcaa aggagaaagc cggcttccat
     1081 caaaagaata tatggaaaaa atgtcagaca aactaggaat gaaaattttt attccagctt
     1141 tgagtatcgg tgtctttgcg atcttgtttg ctctatttac caatttaggt gctttagtcg
     1201 gcgtgggcgt cggtgttttt gcagccatgc taatcttgat gttcttttct cgagataatc
     1261 gaccaagtac atttttagat gatgcagcag atatgctggg cacagtaggg ccgttaagca
     1321 tgttgccaat gcttttagct tctctaggtg cggtatttac aagcgctgga gttgggacag
     1381 tgatttccaa tgcagtaggt acgatcgttc cagaaggaaa tgtgaacatc ggcatcatca
     1441 tttacgctct tggcatggtg atatttacgg taatcatggg gaatgcattt gcggccatca
     1501 ccgtgatgac ggttgggatc ggtgcaccat tcgtatttag tcatggagcg aatccagcgt
     1561 taataggaat ggttgcattg acttgcggtt tttgtgggac cctgctgaca ccgatggctg
     1621 ctaacttcaa tatcgtgcct gtagcgatgc tggagatgaa agacaaatac ggagtgatca
     1681 aaaaccagtt gtttattgcg ttatttatgc tagttttcca aattgcttat atgattctat
     1741 ttaaataata gaagagaggg gttgtgacag aataattgtc gcaacctctt ctaacattgt
     1801 agtggaacac tattttttta taagaatata ttaagtttga tctgttatgt tatttaaact
     1861 attgtttcct ttatttacgc tgttcaagga aaatggtatg ataaaagata accatatgtt
     1921 aaatcaatcg gacggaggaa caacagatga aagtactagt aacaggtttt gatccatttg
     1981 gtggggacaa agtgaatcca gcctatgaag cagtaaaaaa aatgccagat gaaatcagcg
     2041 gtgctgaaat catcaaagtg gaagtaccga ccgtattcga aaaaagcagt caagtagtca
     2101 aagaagccat ccaacagcac caaccagatg tcgtgatctg tgtcggacaa gcaggtgggc
     2161 ggtcagcagt ctcgttcgaa cgtgtcgcaa tcaatctagc agaagcaaga atccctgata
     2221 atgaaggaaa tcagccattc gatacggctt tagaagaaaa cggtccagcc gcttacttta
     2281 cctcattgcc aatcaaagca atgacaaaaa atgtacaaga tcatggtcta ccagcgtaca
     2341 tctcttatac agcaggaaca tttgtctgca atgatatcat gtatcgtttg cttcatttga
     2401 tcgaaacaca attcccggca attcgaggtg ggttcatcca cgtgccattc tcacccgatc
     2461 aagtgatcga tcgtccagtg ggtacgcctt ctatgtcttt agaagacatt gcttcttctt
     2521 tgacttatgc agttgaagca gcagtagaaa ataaagaaga tatttccgga aatgccggaa
     2581 caactcacta atttagcaag aaacactggc tggttgctaa cgttatgtta gtagccagct
     2641 ttttgtaatt agaaaggaaa gaaacagccg tggaagaatg gcaatccgtc tttgaagaat
     2701 ggtttccaaa agaaatcagc aaaagctatc ccatcaaaat ttctaaacaa tacacaagca
     2761 gtcaacgttg ggagatctat gcaaaattaa cgaaaaaaca gcgggaacta gtcgataagc
     2821 accgccgata tctaatcagc tctcgtttta tggaagaaca ttatttagct gcaacagatt
     2881 gggtgttttc tgacttcaag atcaatccgt tttttcgaac aaaacgaagc cagcaaaaat
     2941 tgtattgtga gtgtggtaga gaattaaaag tgcaatacat cgttaaatcg ccaaaaaccg
     3001 gaaagatcct aaaactaggc atcaaccact ttgccgatca cctgcatgtc tctccgacag
     3061 tggcagcttc gatccatcag ggaatgacaa aagttgatct ggccttagac gaattattat
     3121 ggcttaagca gaagaatatc gattttcctg aaggactatg gcaaaaatac tgttttgtct
     3181 tgtaccaaaa tcgtcggatg aaacagccat atttaccgga tataaaatta gctcagcggc
     3241 tcgcagaatt tcgccaagta gaaatgccga tttacatagc ggattatcaa gcattggaaa
     3301 atgaaatcaa gaaaataagt gaacacatca atgggcagtc aaaaaagcga cagataaaaa
     3361 aagaactatt tgacgacttt gcagaagaat tagtaaagga tgtagaagaa ttcttgatca
     3421 actatcgcgc atttttacgt aaagattggc aatccatcgt atatgaagaa gtgccagtac
     3481 atcctaatgc ttattttgaa acatttattt ccgtgttgag aaagacaaaa cgtcaacgaa
     3541 caccagaagt cactgctcag atggaatatt ttgcgaaaaa ccaacgattc atccaaccaa
     3601 aaatctatct atttatttgg aagcaatatt gtcgctatgg ttttacagaa gggttcttcg
     3661 acagcattcc tcggatagtc agaaatggtt ttttaaaagt attgcgtaaa gaacgagaag
     3721 ctatacaatc tgctgataag aaagaccgta cagtctcaaa agaaaaatgg caactagtag
     3781 tgaaagatat ccaatctgga aatgtgcaag agacaatcga caaatggaaa ggaaaacatt
     3841 atcgttttac tgaagctcag aaacaagcgt tggaatatta tcagaagtta gaagaatcac
     3901 tgcgtttcaa tgatgaggca agaaagtatc tgaaggaatt actataaaaa acatactaag
     3961 attagagggg aacatcattg acgaatgatg ttctcctcta ttttctattt ttagattata
     4021 gtgaagagtg aaagaaagtt cgaacaaagc cttgaggact acgagtccct atcaaaaacc
     4081 gaacttcttt actgtcattt tagaatcagg tttacgtatg ttggggccat gagcccgtgt
     4141 ataggaaatt ggaatcgaaa tgaagcagta aagttctttc aaaacttaaa aaggaggaaa
     4201 acaaatgcct tacattcttg cattagatgt ttcaatggga cacagctaca gtgtcctata
     4261 caaagacgac acctgcttat ttgaagacga gattgaacac actcaaagag gatttcatgc
     4321 attactcgaa gagatccagc acttgcctga gcgtccattg attgtttttg aagcaacggg
     4381 gatttattcc agaccagtcg aaaagttctt tcaagatcat cacttttctt actgtttgtt
     4441 aaacccgtta gaggcgaaaa aacagatgga agaaagcacc ttacgcagtt ggaaaacaga
     4501 taaatcggat gcccaccggc tcgcacagac tcatcttaaa aatcaacgtc agccaaaaga
     4561 agtacaaagc aatttttact tagagatgcg cgatttagcc cgtttttatc aagaaattga
     4621 gaagaaaatc actcgtctgc gcatggattt acacaactgc cttcaattga cttttccaga
     4681 gcttgaacag ttcttttcaa acaggctgac cccttacgca ttgacattga tccgtctttt
     4741 tccccatcca gattttgtat tggcatctac tcggacaaaa ataaaaaata aactgatcaa
     4801 cgaaacacgg aaaaaaatct ccgcgaatcg tgccgagcaa aaggcagatc aaatcatcca
     4861 ctatgcacag tgtgcctatc cagctgttga aaaagacagt atccactgtc aaaaaacgat
     4921 ctattacgcc gaacttctcc aagatttact cgaacaaaaa aaagcactgg cgacacaaat
     4981 gatcaaaaaa gccgaaggct ctccttgctt tttgttgtac caaacgttcc ctgggattgg
     5041 cgcactcaca gccgctttat tgctcggtga attaggagat atcacacgat ttaaaaccca
     5101 taaacaattg aatgcctttg tcggaatcga catccgacgg tatcattcag gtaagtatac
     5161 tggccaagac cgcatcaata agcgtggtaa tcccaaagca cggaaaatta ttttcttcac
     5221 cattcgaaat atgattcgcc agcaacgcgc agctcctaat catattgtcg attattacta
     5281 caaattaaaa aagcaaccta tccctaagaa agataaggtt gccacagttg cttgtatgaa
     5341 caagcttctg aaatgtatgc acgctatggt tagggcacat acagagtacg actatgcgta
     5401 cgcggtctca gtggaccatt aactaaacca cttacagtat accacttctt tgcccagatc
     5461 gaaaattttt tacgaacggg gctttatttg gtgtgctttg attttgtcgc tttcgtttac
     5521 gagctatttt tcttgcacct tt
//
LOCUS       DAZKNH010000126         5538 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000126 DAZKNH010000000
VERSION     DAZKNH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.128"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /locus_tag="ACG8MG_002464"
     CDS             complement(<1..302)
                     /locus_tag="ACG8MG_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFQ0376462.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYV"
     gene            395..744
                     /locus_tag="ACG8MG_002465"
                     /pseudo
     CDS             395..744
                     /locus_tag="ACG8MG_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1016..1306)
                     /locus_tag="ACG8MG_002466"
     CDS             complement(1016..1306)
                     /locus_tag="ACG8MG_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376463.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1708..1944
                     /locus_tag="ACG8MG_002467"
     CDS             1708..1944
                     /locus_tag="ACG8MG_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376464.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1951..3903
                     /locus_tag="ACG8MG_002468"
     CDS             1951..3903
                     /locus_tag="ACG8MG_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0376465.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="ACG8MG_002469"
     CDS             complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="ACG8MG_002469"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0376466.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4799..5491)
                     /locus_tag="ACG8MG_002470"
     CDS             complement(4799..5491)
                     /locus_tag="ACG8MG_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0376467.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1801 a          965 c          899 g         1873 t
ORIGIN      
        1 acatacttgc aaagtaatac tacaattcct aacataatcg tagaatttaa taatttccat
       61 agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct tgaactccat
      121 gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct acttgcataa
      181 actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa gataccccaa
      241 atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt atgtttgttc
      301 atttttatac tccttcttat atttcctaat agagtataac atagctttaa gcattactta
      361 aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat ttgatgcaaa
      421 agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt tttcttagtt
      481 aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa gacatacaat
      541 catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa aatccaaatg
      601 ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac
      661 ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca ggaacgttta
      721 aaatcctaca attccactac ctgagactag taatgactag tctcttttta tggcctctga
      781 tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc tttaagcaat
      841 atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa tacatgtagt
      901 tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg
      961 catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt gttttctagt
     1021 ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca tattctacca
     1081 caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt ctattattat
     1141 aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag ttcaaactat
     1201 gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt ctggcactaa
     1261 cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc ttcacctcaa
     1321 taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta tctttttgac
     1381 tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat gaaggacatg
     1441 tactaaaaaa aagaggcagc agtatagatt taagtagttc agttttggag tacaaaaagt
     1501 gaaaatgtaa acctaaatga gtatcaaatt gcaactgaat ttgatactcg ttttttattg
     1561 ttaaatcaac gtttgttgtt aatttacacc acaaaatgta agttaaaatg tatataaaaa
     1621 tgcgaacgtg aaataattat tgtaggaatg caaattggtg tgttaaaatg gttacaattt
     1681 actaagcaaa aaggggtgca ttttcctatg caatttcttg agccaaaaaa taagaacgct
     1741 aagtctgtgg attgggaaat atcagagcaa gtaagggtga tagttaagca atacgctgaa
     1801 tatgcagagc gtactgaaag cgaagcagtg gatgaattct tattgaacat tttagacgat
     1861 aagaagttta ttgaatggat tgccaacaag cgaagtaata agcggattgt tgaaaaaatg
     1921 ggtatcaaag atagagtggg ttgatagaca atgaccaagc ttaaaagatt agccacgaag
     1981 gaagatgaaa ttgtagatgt taaaattccg atttcaaacg aagaattaaa agatagggct
     2041 aaacaatatg atttattaac tccaaaacga tttgcaacaa ggtataacaa aatgttgttc
     2101 ttgccaacgt cttttaaatg gaacggttct gaatatccca ttcaatataa ttactgcatc
     2161 aatccgtttt gttgtaattt tgggaaagaa cagcacaaat tcaaagatgt aaaagggaaa
     2221 ccaagtcgat ataaaatgac tggtagttcc aaagataaag gtcataaagg tatgtactgc
     2281 aatgacaatc ctattggcag aggtgtatcg caaaactgta cagtaacccc tttatctaat
     2341 tggtctgttg ttgaagaaat taaacgcctt atagaaatca atagcatcca agatgttgag
     2401 ccagattatc aatttcataa agaaggctgc tcggaagaag aatcaacccc attcaatgag
     2461 ccgaagcaat tttacaaaag ggggaaaagt aggggcaaat ctcaacgcta tcaatgtaag
     2521 gcgtgtaaga agttcactaa tgttctacca aaaagagaag aaaccactac ttatcaccaa
     2581 caaaagaaca ccattcttcc aatgttcgct aagatggttg ttggaagggt ttcagtcagt
     2641 cgtacttgtg atattttagg aattggagtt ggaacctact atcataagct ggagtggctg
     2701 tataggcgtt gtttggagtt tttagaacga tatgagacac aaccccttca aacaaagaaa
     2761 tttaacgaga tgtggcttaa tacggacaag atgcactact acctaaataa cgtccgtaag
     2821 aaaggacaag gttcaaagaa atatacgggg tttgaagacc ttaatatgca aacgtatata
     2881 gttgtatctg ctgaggtctt atcaagatat gtgttccgtt ctgatgttgc ctatgattgg
     2941 aatatttcaa tggatgaatt aaatgaagat acaagaaaat tcaaagaaga ccatctcaat
     3001 acttttagtc gtaaaaatga taggttagat tggtcttatt atccacaaga accatccgct
     3061 aacgatagcg aaaataggaa tgcttatctt cacgaattag ggaaaattac taataggagt
     3121 agatttgtcg atggtttaaa tgttgatgct ccttatacta caactgctca ctattggtta
     3181 attaaacaaa tggttaatgc cgacgaatgg cgaatgatta gtgatgatga cttctctatt
     3241 agaaacgcct tttatagagt attcacgaaa gaattaaggt tgagcgatgc acatcatttt
     3301 atttgccaag ttaacaagac gaaaagcaga aaacaatgct taaaagaatt cggacaagca
     3361 aaagcagaac tgctcgattg gggagatatc agaggattca aaacaaagtt tctacggact
     3421 ttggcatctc attatctaac tgaattacta acaagtcatc aattccacga agaggctatt
     3481 agtaaagatg gagaaagata tcgcaaatat gccgataacc ctatcaaaca tccattagcc
     3541 actaaggata aaggatttta ttcagttgat tgtagaacgg atttatctgc tctggaacca
     3601 aacgaaatcg caaagatgct attaaatgtt aatgaccatt cgaccaatag ctttattcaa
     3661 cagataagac gttatatatc atctcttgaa agaccattaa caacagctcg tggtgataag
     3721 aagagctata tttacgctaa cttcaatccg aagtatgctc aatttgcgat tacaattcta
     3781 agaacttact acaacttttg tcgccctttt aaatcagcgg ataaaaaggt tttgacacct
     3841 gctcaaagat tagggattac agataaacaa tttgattgga aagatataat ttatttcaaa
     3901 tgacaaaaag agcttggagt aatcccaagc tcttgtgctt tatctttact gttcttcttc
     3961 aattaacgca cccgttaact caataagttt tttctttcat atacatgaaa atagtagtta
     4021 tacggatttt tatcattctt tatccctttt tgggcaaata cctcattcca ttcctcataa
     4081 ttcacttctg gaaaaaaagt atctccttcg aattcatgat gtatttttgt gatgtacatt
     4141 ttctcaacat aagggaaaaa caaattataa atctgttctc ctccgaaaat aaaaatttct
     4201 tcttcgtttt tacataactc aaaaacatct tctattgaat gaacaatttc acaaccatta
     4261 aaggtaaacc ccttatctct cgtcagaata atatttcttc tgtcaggtaa ggctcttccg
     4321 attgattcaa ggttcttcct acctaatatt atcggatgtc cctttgtagt atttttaaca
     4381 tattcccagt ccttgggaat cctccaagga atgtcattct ctttgccaat cactctattc
     4441 ttatccatcg cagcaatcaa agaaactttc atttataaca cagccttttt aactaattta
     4501 ccatctaatt tcttatttct atcaaaaaac cttttctcat tatcctgccc gttagttgaa
     4561 taagcaattt tttctataag ttatattatc ataataccac aaaatcacaa attttcattc
     4621 aattatcatt ttcaattgca ttttataaat cattttgtat gtaatttggt atgtaagtta
     4681 aaatgaaaaa agtagtccat tttgaagtac attttgtcat tttgtactcc aaaactgaac
     4741 tacttagtat agattactgt tgcttcttta ctaatttagg tttacttgtt aagaaagatt
     4801 atttacgcaa atgttcttca acaatttgct gagatttatc agctgtatct tccacttcat
     4861 cttttgcttt ttctaacttt tgaatatatt gtttcaagct tgagttttca gcagttgctt
     4921 cgtctaattt cttttgtgta tctgttaact gagtttctac atttgttttt tcttgtgtca
     4981 gtgttgcgat ttgttgattc aaaccattta cagtattttg taattctgaa agttttgctt
     5041 gtgcttcttg tagtcttgct gtccaatctg cattcagatg attgatttca tcattttttt
     5101 ggttgatctc atctagtttg acattgattt cattttgttt gctttgtagt tgtgcatcct
     5161 tgctgactaa ttgactttct aagttttgtt tttctgctgc cgattgattt ttttggtttt
     5221 ctaattcatt tacttttgtt tggtattctt ggacatctgc ctttgaatct tgcaatgtct
     5281 gttctttttg tgctagtcga gcggtaagat ctgtaatcgc ttgctcttta tctgtcaatg
     5341 tatgatcaag ctgtgtcaga ttctcattga ttgtatcaat atctgtctct ccaccccaca
     5401 aaataatttc atttgcgtac cgcgtgcctg tgtagccaat tagacataga aatgcaacga
     5461 caatcccacc agtaataaat ttctttttca tttgcatagc tccttaatcc aatactttta
     5521 catgctaaaa gtaagtga
//
LOCUS       DAZKNH010000127         5446 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000127 DAZKNH010000000
VERSION     DAZKNH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.129"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..702
                     /locus_tag="ACG8MG_002471"
     CDS             <1..702
                     /locus_tag="ACG8MG_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0376468.1"
                     /translation="DPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEELLNAAKIRTS
                     SFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILKSNIEPTDYI
                     VKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDIQYFSTSLGA
                     PHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKKTREVTLANG
                     ETIPASIRGLKKLIS"
     gene            complement(771..2294)
                     /locus_tag="ACG8MG_002472"
     CDS             complement(771..2294)
                     /locus_tag="ACG8MG_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0376469.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            2546..3727
                     /locus_tag="ACG8MG_002473"
     CDS             2546..3727
                     /locus_tag="ACG8MG_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0376470.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3787..5415)
                     /gene="treC"
                     /locus_tag="ACG8MG_002474"
     CDS             complement(3787..5415)
                     /gene="treC"
                     /locus_tag="ACG8MG_002474"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFQ0376471.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
BASE COUNT         1484 a         1083 c          950 g         1929 t
ORIGIN      
        1 gatccaaaac aattagaagt tttatcaacg gtgatcaaaa atagaatcat gattgaaaac
       61 ttagacagtt atgtccatat ttctacctct gatccggaag aacttctcaa tgcagccaag
      121 atcagaactt cttcttttgg tatttacttt ttagatatcg aactagaaga cagtgaaatg
      181 gatggtgtag cgatcggtaa attgatccgt gaattagatc ctttaggaaa aataatctat
      241 gtgacttccc atacggaaat gtcgatgaaa atcttaaaaa gcaacatcga accgactgat
      301 tatatcgtca aagaagatct ttatgattta aaagaaaatg tcgagcatat cctgagcaaa
      361 attttcgaag attttcaaat gtcccaaatc caaaaagata tcttcaaaat agagtttaat
      421 gatgagatca aatttttacc gatcaaagac atccaatatt tttctacttc gctaggagcg
      481 ccacataaac tggaagtcca tttgactcat gcccaattac aattctatga gaagatcaaa
      541 gaaatcgaga aaatgcatcg gaattttatc cgttgtcata aatcctatgt tatcaataca
      601 caaaatgtgc ggtcgattaa taaaaaaacc agagaagtca cgttggcaaa cggcgagacc
      661 atcccagcct ctatccgtgg attaaagaaa ttgatttcat aaaaagaacc gaatagtttg
      721 gaccgactaa atgaccagcc gatccaaacc cttcggttct tttttgccta tcagtgcaaa
      781 agcatttcct gtgcaatttc acgtccatcc attgtatctg gataaaatgt tggccaattc
      841 tccatttctt tcagcaatac ttcttgactt tcatttcctg catataaatg aaaatgttcg
      901 gagtcagccg gtgcaatcgt gtgatcactg aattgaatat atctaaaagc gccactttcc
      961 ttatcttctg cttcgaatag ataccttacg ccacggtttc ctgcctcgta ggtcaagatt
     1021 tctttcccta cataacgata atttgaacgt ttcgttgttc cattcttaaa cgtaaatgcc
     1081 atctctttct cactgatctt gattgaagca atatctgttt gatagccctt cgtataatat
     1141 tctttatatt cttgtgctgt cttatcctga ttcaattttt ctttatattc aaaaacagga
     1201 tcaaaagtcc catcttctac atacgtatct actgtttgcc aagttcctgc ccaatctgaa
     1261 agagagcgat cttgtacatc ctcgtcttca aaataaccgt tgtagacgct cttttctttt
     1321 ttgggggtca aagtatcttc tagcgggttt tctgtctctg tcgttttttt caacgcttcg
     1381 aggtttgctt ccatgataga aagatagttt tctccatttt ctatttgttc attcgtcaat
     1441 ccttccaaag gattcaatac ttctaaagat acacctgctt cgtttgctaa cgttctggca
     1501 atactatctt tggcgttttc ttcaaaataa atatagttga ttccatgttc tttaacaaag
     1561 tgtttcagtt cagcaattct cgccgcagac ggttcttcac tagaagaaag accagcaatc
     1621 gatacttgat tcaatccata ttctaacgct agatagttga atgcagcatg ttgcgtgaca
     1681 aaagatcttt gttttgcttg cttaaaggtt tcttgataaa gctgatggag tgcttgaagt
     1741 ttctcggtat aggctgctgc attttttgtc catacttcct gtttttctgg gtaagcttca
     1801 atcagctgat cacgtatgtt tgctacctgc ttgattgcta aacttggagc tagccataca
     1861 tgtgggtcta gctcatgtgt atggccttct tccccgtgtt catggtcatg atcatgtcct
     1921 tcttcacttc ccggaagtaa aagcatacct tctgtcgctt ttatcgtatg gacattgcct
     1981 tcttgtaatg ttttttggat tgcggggacc catgtttcca tgttttcatc attataaaca
     2041 aagatatctg tatcttggat ctttgccatg tctttagcag aaggttcata atcgtgggaa
     2101 tcagtccctg ctggaatcaa taactctact tctccttcat ccccgacgat tgcttttgta
     2161 aaatcataca tgggataaaa ggtcgtcatc acgctcaatt tttctttact gtcctcggct
     2221 ttcccacttg ccccgcaagc agttaacata cctgctaaaa ccagcatggc caatatacct
     2281 atcaatcgtt tcatttatga cactcctctt ctcatcttct ttcatttgaa tgttccgatt
     2341 cgcttttcgc aaaccgtaat agttacgttt tagcaacagg atttaattta tcatatggtt
     2401 aaaagaactg tcaagcgttt tcttatttta attgatgaga aagtttatat gaatgattga
     2461 cgatattttt gcaatcaacc tctatcattt tccttttata cccgcttttg tctataatga
     2521 atttatagat aaaggaggaa aaagagtgaa aaaagaaaca gatacaacga atcgtttgat
     2581 ccgcttttta ggaggaaaaa cctcttacta tgttttagga atagttatct tacttgcgct
     2641 gatcatttat tttttccatc agatctcgtt tatattcaag cctgtcggaa tcattatcgc
     2701 tactatcttg cccccattat tgtttgcact gatcttgtat tatcttttgg aaccagtcat
     2761 caaacgtttg agcaagcacc tttcgaggaa cttgtcagtc attatcgttt atgtgattat
     2821 tttattttta ttggggcttg gaagcatatg gcttattcct tttcttgaag aacaggcaaa
     2881 gacattgatc agtagtttgc cagatttgtt tcaagatttt caagagagtc tccgacaatt
     2941 tttggagaaa acaccttttg ccgattcatt taatcaattt ttcgcttcta ttgacgacgt
     3001 gacgtctgat atagctggat ttattggaaa ttattgggaa agtggcgcac aacggatcag
     3061 caacattttt tcaactgtta cagctatttt tattacttta tttactggtc caattgtcgc
     3121 ttttttcctt ttgagagatc ctcaaaaatt ttatcagtca gttttagcga ttgtcccacc
     3181 agcatttcgt acagatttta aaaatttgac aaagattgct aatcaacaat tgggttcatt
     3241 tctaaagggg caaatcattg cttcatttat ccttggagca gtctactggg tttgtttctt
     3301 actgatcgga ttagaatttg ctagtgtttt agctatttct gctgggttgt tatgtatcat
     3361 tccatatatt gggccgttta tcgccttctt tccaggtttg atcattgcat tccaagactc
     3421 tacttttatg gctgtcaaat ttgtcattgt ctggtttgcc gtacaacttc tccacggtga
     3481 cttggtgatt cctcgagtaa tgggtgatcg tttacagatc catccgatta cgatcttaat
     3541 cgttttactt gtcatgggtg atctaatggg aattgtgggc gttatttttg gtattcctat
     3601 ctatacgtta gtcaaattac tcgttacttt tattttccgt aaattcaaac aacgctacaa
     3661 taaattttat ggcgataaag gagagtatga aaactcttca ttttctgaag aagattattt
     3721 taaataaaac acaaaacgtc gatcttacga gaatagctcg taggaacgac gttttgtgtt
     3781 tttgttttaa tttttcagca aagcaattgc ctggtatggt tttagctcca gactttgatg
     3841 aagaacagta tcggaataat tatcgataag aatattggct tctgtgaatc cttctgggat
     3901 ggcgatcctt gtatctttat catagaaatt tgttaataca agcagctgct gtccttcaaa
     3961 ttggcgaaga aagccataaa cttccggatg atttgcttca taagcatgat aatctccttg
     4021 tgcaacgatt ggatatgttt ttctcagccg aatcaatttc ttgtaatagg acaaaataga
     4081 atcttcagaa gatagttctt tttctacatt aatctcttct agcttgcctc caacttttaa
     4141 ccaaggtttc cctgtagtaa atcctgcttg ttctgtgtct gaccattgca tgggtgttcg
     4201 agagttatcg cgagattttg ctttgatccg acgaaacgct tctttttctg tgaatccttg
     4261 ttcaaggagt aacttataag cattcaaact ttctacgtca atatagtcgg aaatcgatga
     4321 atagtctggg tcgatcatgc cgatttcttc tcccatataa atatagggcg tcccacggtt
     4381 caaatggatc atggttgcta gcatctctgc tccttgtttt cgatactgat cgatagctac
     4441 aaatcgattg agcgcacgtg gctgatcatg attattaaaa aatagagcat tccagccgtt
     4501 accctcactc atcttttctc cccatgtatg aaatagtcgc tttaattctg caaaatcaaa
     4561 tggaggagta gaccatttat tcccattttc ataatcgact ttcaaatgat ggaaattaaa
     4621 gaccattgat aattcttctc tactaggttc tgtatacaag atacaatttt cgatcgtcgt
     4681 agagctcatt tcaccgactg tgatgatagt ggaatccgtc ccaaacgttg cttggttcaa
     4741 ttcttttaga tacgtatggg tgattggttt gtccgtatat tcaggtttgc catcattttc
     4801 tttattattt ttcaattgtt cgtctttacc gataacattg ataacatcga aacggaatcc
     4861 ttttacccct ttttccatcc aaaaacgaac aactttgaat agttcctctc ttactgcttg
     4921 gttccgccag ttcaagtcag cttgtgtccg atcatataag tgcaaataat atttccccgt
     4981 atctccaaat ggcgaccaag cacttccacc aaattttgat acccaatccg ttggctcttc
     5041 tcttaaaatg aagaagtcct gataatgctg gtcccctgct agtgcttttt tgaaccattc
     5101 atgttctgtc gaaacgtgat tcagcaccat atcgagcatg atttctatcc ggtatttctt
     5161 tgcttcggct attagctgtt cgaattcctc catcgtacca aagataggat cgatggctgt
     5221 gtaatcagag atatcataac cgttgtcttt ttgagggctt ggataaatag ggtttagcca
     5281 aatcatgtcg atgcctagat cattcagata cgggagtttt tctttgattc cttttaaatc
     5341 tccaattcca tcaccattac tgtctttgta agatttggga tagatttggt aaatgacttt
     5401 gtctttaaat gtcatggata tttacctcca ttcaactgat ttatta
//
LOCUS       DAZKNH010000128         5312 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000128 DAZKNH010000000
VERSION     DAZKNH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.130"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8MG_002475"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8MG_002475"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFQ0376472.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACG8MG_002476"
     CDS             complement(1297..2133)
                     /locus_tag="ACG8MG_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0376473.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACG8MG_002477"
     CDS             complement(2317..3534)
                     /locus_tag="ACG8MG_002477"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0376474.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACG8MG_002478"
     CDS             complement(3683..3844)
                     /locus_tag="ACG8MG_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376475.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACG8MG_002479"
     tRNA            3917..4000
                     /locus_tag="ACG8MG_002479"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5312)
                     /locus_tag="ACG8MG_002480"
                     /pseudo
     CDS             complement(4240..5312)
                     /locus_tag="ACG8MG_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1654 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca ta
//
LOCUS       DAZKNH010000129         5189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000129 DAZKNH010000000
VERSION     DAZKNH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.131"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..748
                     /locus_tag="ACG8MG_002481"
     CDS             137..748
                     /locus_tag="ACG8MG_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0376476.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="ACG8MG_002482"
     CDS             821..1750
                     /locus_tag="ACG8MG_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFQ0376477.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1887..2645
                     /locus_tag="ACG8MG_002483"
                     /pseudo
     CDS             1887..2645
                     /locus_tag="ACG8MG_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
     gene            complement(2693..3895)
                     /locus_tag="ACG8MG_002484"
     CDS             complement(2693..3895)
                     /locus_tag="ACG8MG_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0376478.1"
                     /translation="MHRKEDFHMNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKT
                     ELTAFLDYEKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYR
                     RTNDTLEETVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERE
                     LHDRYAAIYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEI
                     LIDLQERGVKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKE
                     VCDDFKMVYQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKT
                     IYSTNLIESFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTD
                     TLASMFTE"
     gene            3975..4499
                     /locus_tag="ACG8MG_002485"
                     /pseudo
     CDS             3975..4499
                     /locus_tag="ACG8MG_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4706..>5189)
                     /locus_tag="ACG8MG_002486"
     CDS             complement(4706..>5189)
                     /locus_tag="ACG8MG_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0376479.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1639 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttt
//
LOCUS       DAZKNH010000130         5136 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000130 DAZKNH010000000
VERSION     DAZKNH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.132"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..446)
                     /locus_tag="ACG8MG_002487"
     CDS             complement(<1..446)
                     /locus_tag="ACG8MG_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0376480.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
     gene            complement(753..1850)
                     /locus_tag="ACG8MG_002488"
     CDS             complement(753..1850)
                     /locus_tag="ACG8MG_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0376481.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1958..3985)
                     /locus_tag="ACG8MG_002489"
     CDS             complement(1958..3985)
                     /locus_tag="ACG8MG_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFQ0376482.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4130..4858)
                     /locus_tag="ACG8MG_002490"
     CDS             complement(4130..4858)
                     /locus_tag="ACG8MG_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0376483.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1456 a         1039 c          813 g         1828 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa ccatcctaca
      721 ataataaaaa tttactattt gttatcttaa ccttactttt ctttctcttc tttttttctc
      781 aaaacaaaca aaagtatgca taataaaata aacaataaga aaagtgaagc tactaagata
      841 taaatccaat tattgtcttt tttaatcgta acatccgtcg tgttcaattc cttggctact
      901 tttccgtcta ttgtaaaatc tttttcaaat acccattgat tggtatagtt gatcgtagta
      961 tttccttttt ttctcgaaaa ttttccattt tcattttcat ttcctaaaac agtcattgta
     1021 aggtgatatt cacctggggt tagcggttga ccatttaaag cagttggaaa agaaaaattt
     1081 gtattcggcg caatctgcaa tccttctttt tcctcttgat ataaaacttc agaatgtcct
     1141 ttttttgtaa tttttgtttc aataactact tgattgatat aggttttttg atcattttga
     1201 acgtttgcta agatgacatt tcttgcattg atttgatctg gtttaacttc atgaagagta
     1261 aggttcggag caacttcgtt cagtgtctgt tgcatcaaca aagcaataat ataggtgtat
     1321 tcattctcaa tcgataagct ttgatcctta gtttctgttt ggtccttttc cgtctggatt
     1381 tcttggaatg tgatcccacc agccaataca ccatcaaatc gctcattcgg catcctgaca
     1441 tgaaaagcaa cggtttggac acttttaggt tttagcgtca ctatctgtgg atagctcaca
     1501 taatctacca gatcaaagat caggctcatg tctttttcaa tctcgttttt tccgtattca
     1561 accatcacat ttgaattggt ggttgcgcta ttcacagaaa tatcgatttt cacttctttt
     1621 tctgtatcat ttctcaattc cgcttttact tctacttcct gatccggatc aagttgaaga
     1681 tcgaagtaag tttttctctt gtcgatttga ttctctgaag ttatcggcgt cactgaaaaa
     1741 ttaaatgcac tagctgacac tgatatattg ttaaaaagtg cccctaaaat aactaggagt
     1801 gttataaata attgttgttt tggtctcaag aaaatcgctc ctttaatcat atgtaagaga
     1861 agactataac aaaatcttgt ctctgtggga cacaagagcc tatggtacct gtgcccttct
     1921 tgaaactcaa taattttgtt atattcttct cttcctgtta atttcctggg acatctgcaa
     1981 gtatccaaga tagtttcccc tcaaaggttc cgattctttg gttttctaca ggaatattta
     2041 ttctgattcc acgatgagct gtagaatcta aaagagagga caaatcgctt tcaccctttt
     2101 gatcactttc gaaaatcgta acagcggatg tccctataac cgttgtattt tcagaggtaa
     2161 caaaggaaag agcttctgga agctcaaaat ctccatttgt aagaactttc tcttgtttca
     2221 attgtaattt ccaacttcca aaagcccgtt catctataat tttcaaccca cctgaaaact
     2281 gtccgaattc acttaatggt ctactggata ttttatgttt tccaaaattg acatattgag
     2341 gagcagttga gagataaagt ttgccttttc tgtaataaaa atagatatcc tctgtatttt
     2401 cggagaagat gccttctgta ttcgctgaat ccgagtcaaa aacataccct tcaatatcaa
     2461 taggagtttc tttatacttc tctcctattt tcccagtgat tgttttcatt tcagcaatat
     2521 caatctgact gtctttatct ttataataaa catggatatc tatctccttc agctttgcga
     2581 tctttaggtt tatttcattc gattgctcaa tcaaattccc gttttcaagg tattcaaaat
     2641 gcatagtgag tactttttca ggaattactt cctgcaaagt tgtctccaat tcgattatta
     2701 acgtatctcc agcaataaaa taatctgtca gatttagcgt gactcgattt ccggttatca
     2761 agatatcttt ggcatcaatt tgatttccat tgatcaagat atgctcggga atagttaatt
     2821 cttgtgggag gtcgatacta gtctgcattt tcttatattc aagaaaatcc ccctcgacct
     2881 tcaatgtatt tctgatagtt atcttatcgt ttcctccagc ttcagcgatg gccccataat
     2941 cactatcgtt gacttttaca tcagctgttg atgtgacatt cgatggaagt cgcgtgacag
     3001 cgacagcatt ttcttgaaaa aaagtacctg ttgaagaagt aaacccccaa tttacttctg
     3061 tagaaccaaa ctgcctaatg agatcaggta taatccgaga agagcgaccg acaccttcca
     3121 aatcataagt aagacttctt tgctccgcgt cccatttaat tattaacaag cgccatgtgc
     3181 cattagaaag aaatgtagga gctaccgtca cgccagtatg ctctccagaa taattattat
     3241 tatttggttt aggcgtgaca aaagcgacat gtccatgccc tctattatca gcagttatct
     3301 ctcgatccat tcgatcagaa gaaccactat ttttatacgt atcaaactca atcgagagcg
     3361 cattcgcaat gtaaggttgg ccagaacgag tagaatatga gccaattccc atacctggcg
     3421 atccaataac ttgactggga ttactagcca ttctaggatc gccagtcaat gtaaaggtca
     3481 tgccatctcc cgctctatct cgttgatttc ctaagtataa ataagattta aatgtaaaat
     3541 ccgagagtaa actaatcttc ttatttgacc aaatagctcc tctttgagac ctttctgctg
     3601 ggttcaactg cagtaacttc ccattttcca atatggacgt gctagtaccg ataggtgttt
     3661 gaaaaatttt atcgatagaa ataatattgg gatcgtcagg cagctcaacc gcagaaattg
     3721 gtgcatcttc tagtccttgt ttgtccgaac cctcagcttc tacgttgtca gtggtctctc
     3781 cactactcga aatacttaaa tcctcactgc tttcttgtgt ctggttggat tctattaaac
     3841 tgtcgaatgt cgaagtgagt ttgctttctt cgtctcttct atttccatgc tccaactgat
     3901 tcaattccaa agagtaccct atgatcggaa agatattagt cactaagaga ctgcttaaaa
     3961 tgagcacgat cctcctcttt ttcaatatat cttttcctcc tgatcgttaa attcctagat
     4021 gaactattcg tatccaaaca aatttagcct agcctttgac aatttttgct cgattacctc
     4081 gcaagacaat atgactacgt atttattttt aacagttctt ttcagtaaac tagttgttag
     4141 gtgtatcttc taatgtccaa actaatttag tggaatattg ttgcgctaac ttgacagatt
     4201 taccgggtac cgaaagctgt accgcatctt gattttcatt ttcatttgca ccaaatcgat
     4261 agacccaagt ccccacacct gttcctgatt tagcattaat tgctaacgta ttgtttcctc
     4321 ccggtgtcaa cgttaattca gaattcaccg tttgcggcgt atatgttgga tcaaccaaag
     4381 aaacagcctg ccctttcgta aatttcagct gtgctccaac tagttcatcc ccactggcag
     4441 ttttaaactg ttctggttga gagacactta atttccaccc agataaggta cttctcctat
     4501 ctgtcacttg gacatatgtc ggttttctag acccatcgtt catcacatca gcagctgcat
     4561 aatacgtttt atccgcagta gaaatttttt gagtcccaaa tttgaatttt gatccataat
     4621 caatcgataa tgcccctcct gttccagggc ttggaggatc tgatggatcg acaggtttat
     4681 tgggatcagg attctctgga tcaacaggag gagttacacc ctcatctcct gcttcaaatg
     4741 taatcgttcc ttctgtgtta tattcctttg ggtatacttc agttgcagaa acaaaagtcg
     4801 tacttgctac catgctgaat aacatcattg ctgctgttaa tacgatttct tttttcatca
     4861 ttgacattcc tctttcttta tttactaaac atatcagtcg attggattaa tccatagcaa
     4921 aattctgttt tacgatactt atgaaataaa agaacataga cctatatttg gtcctatgta
     4981 tcgattagtt ataaatgatt atcagcccga tttgatttct agtttttatt gcaacttgat
     5041 gcaaacacaa tctagaaata cagctgtcat agcctttaaa ataattcgta agtccccctc
     5101 ctatctatca aaatgtgact tgacgagagt gtaaaa
//
LOCUS       DAZKNH010000131         5005 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000131 DAZKNH010000000
VERSION     DAZKNH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.133"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..877)
                     /locus_tag="ACG8MG_002491"
     CDS             complement(104..877)
                     /locus_tag="ACG8MG_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0376484.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1021..1371
                     /locus_tag="ACG8MG_002492"
     CDS             1021..1371
                     /locus_tag="ACG8MG_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0376485.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1352..2068
                     /locus_tag="ACG8MG_002493"
     CDS             1352..2068
                     /locus_tag="ACG8MG_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFQ0376486.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2068..3516
                     /locus_tag="ACG8MG_002494"
     CDS             2068..3516
                     /locus_tag="ACG8MG_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0376487.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(3587..4096)
                     /locus_tag="ACG8MG_002495"
     CDS             complement(3587..4096)
                     /locus_tag="ACG8MG_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376488.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4086..4810)
                     /locus_tag="ACG8MG_002496"
                     /pseudo
     CDS             complement(4086..4810)
                     /locus_tag="ACG8MG_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1722 a          854 c          999 g         1430 t
ORIGIN      
        1 ataaaaaagc gaatcatatc ccattattta tataataact gcccctttct taagaaaaaa
       61 taagcgattt tcttaagaaa agaggtagct tcaaaaaaag atcttaaatc agatcaaaat
      121 acttcaatgc gtggacaatt ccgccttctg tatttttctt tgtgataaag gtagaaagtt
      181 cttttaatcc gtcataggcg ttgcccatag cgattttgtt gtcgcaagct tccaacaagg
      241 caaaatcatt cggaccatcg ccaaatccaa aagtaggaac tttttccagc tgaagttctt
      301 tttgcagacg tttgacacct gccccttttg agattccttt cttcactgta tcgatcgagt
      361 aaggaccatt ccgataaaaa gttagctcag gaaaatgttc gtgatagtat tcgtcacctg
      421 attgagataa gatcagcaac atatttactg tgttttcttt aaagcctaaa ggatcaattt
      481 ccggaacatc tgaatgaatg aagtcgtaag cattgatcat tgtttggcta tgaccggtac
      541 accagatttt ctgatcgtta tagtaagaaa gttcatgacc gttcaactgt acatgatcat
      601 acattttttg acattcttct gaagaaaatg tttgagaata gatttcttcg ccgtctactc
      661 gaatgaaaga accattcatg gtgaccgcag aagtaatgcc cgcatcttcc atgatagagt
      721 ggatttctgc ttctgtacgt cctgtagcga tgacggggag aacattgttt tcttttaatg
      781 cgttcatagc ttttgccact tcgggtgtga tttttgattt cgcatctagt aatgttccat
      841 caagatcaaa gaaagtgatt gcacgataat ttgacacaaa taaaactcct tttttattgg
      901 aataagttta tcttaacaaa gaatgaacaa aagacaaacc tttcgtgaag aattcctgaa
      961 catattggtt cagtcaacaa tttagagtat actataagaa gagaatttag gagggaaaga
     1021 atgatttttt tcagtattgg tatcttattc ttaataattg gcagtctttt tcggattctg
     1081 ccctcaaaag gaaatctgcc tttttatggg tatcattcgc ctcttgccgc aaaaacagac
     1141 gctcattggc ggctggctca aaagacaagc ggaaactggt tttttcttat gggcttactg
     1201 atggcattga tcggctatta tttaaagaca tctgggcata cgaattattt tctgattgaa
     1261 atgcttctgc tagtatttcc aattatgcct atttttatta tgacagaaaa gaaattacag
     1321 aaatatgacc tggaaactgg agggaacgat aatgaatatt ttaatgattg aagataatca
     1381 atctgtttct gaaatgatgc agatgttttt cttaaatgaa ggttgggaag caactttcaa
     1441 gtatgatgga aaagaagggc tcgatgcctt tttagaaaac ccacaacatt gggatatgat
     1501 tacactagac ctgaatttgc ccacactgga cggtatgcta gtaggaagag agatccgcaa
     1561 agtttcaagt actgtgccga ttatcatgct gacagctaga gattctgaaa gcgatcaggt
     1621 gatcggctta gagatggggg cagatgatta tgtcacaaaa cctttcagtc ctctgacgct
     1681 tattgctcga atgaaagctt tgcatcgccg tagtgagttg gcagaagcaa gagaagaagc
     1741 agtagtttca gatgatcatt ttgatatcga gactagtcat ttcaagatga atacgaaaac
     1801 acgagaagcg tatctggaca accggccaat tgaaggactg acgcctaaag agttcgatct
     1861 attgtttact ttagcaaaaa aaccacgtca agtattctct cgggagcagt tgcttgaatt
     1921 agtctgggat tatcagtact ttggtgatga acggacagtg gacgcccata tcaagaaatt
     1981 acgacaaaaa atcgaaaagg tcggaccaca agtcatccaa actgtttggg gagtcggcta
     2041 caaattcgat gattctggtg ttgcctaatg cgttatttgt atcagcaact cttagctttc
     2101 tgcggaatgg tcgcgttgat cttattgatc gttggagttt cttttacaca actgacaaaa
     2161 caaacgattg aagaaaacaa ttaccagcaa ctgtttggat atgctgaatc agttgaaaag
     2221 acaacgcaaa cctatgccga tacgctcccg cagtttagtc aagatcaggc gtttcaaaat
     2281 gcattgctgc tgacagagca agtactgaca gaacaaaatg tgaactttat atttatcgat
     2341 aaaaacgaac gtgtcattta tccgactaat acagctttac tgaattttac cttaacagct
     2401 gatcaatgga aaaatctgag gaatggacgt caagaaaaat tcacatcgaa taaaaatatt
     2461 ttaggacaag cacaggcgac ctcttacgca cttgtgccat ttaatttgaa tcatgaattc
     2521 tatggagcac ttgtagttag tcaacctgcg cgaaatatcg ataacagcgt tcgtccaatt
     2581 acactgaacc tgttcaaagg atttattttt tccagtatcg ttgcagttat tgccagctat
     2641 gcttttgcag cattccaagt taagcgtatc aatcggttaa ggaatgcgac gaaagaagtg
     2701 acgaatggta attttgatgt acagctcccc gtacatgaca aagatgaatt tgatgagtta
     2761 gcagatgatt tcaataaaat gacgaattca ttgaaagaat ctcaagcaga aattgaagaa
     2821 caagaaaatc gtcgaagaca atttatggca gatgcttctc acgagatgag aacacctctt
     2881 acgacaatca acggacttct ggaagggtta gaatacaatg caatccctga aaatcagcga
     2941 gaaaacgcga ttaaattgat gaaaaatgaa acagagcgtc tgatccgttt ggtgaatgaa
     3001 aacttggatt atgaaaaaat ccgtacgaat caaatcagta tggtcattaa aaaattcaat
     3061 ggaacagaaa cattgaaaaa tattgttgca cagttagaag ctaaagcaga agcagcagga
     3121 gatacattga cattgaaagc cgttgatgat attgatgttt acgctgatta tgaccgattt
     3181 gtccaaatca tggtcaatat tattcaaaac gcgatacagt ttactgaaaa cggccaaatc
     3241 acgattactt tagaaaaagg ctatcttgaa acgatcatca cgattgaaga tacgggaatt
     3301 ggaatgtctg aacaacaaat gaaaagtatc tgggatcgtt attataaagt agatccttca
     3361 agaaaaaata caaaatatgg tgaatcagga ttaggtttac caatcgtgca gcaacttgta
     3421 cgcttgcata aaggaaaatt agaagtagaa agcgaactag gaaaaggaac aaaattcgct
     3481 gttcgtttgc ctgacgtaga aatcaaaaaa gagtaaaaaa caagcggtat aaaagatcat
     3541 gaatcgatct tctataccgc ttgtttttta ctataaaatg attgatttag cccgcgggct
     3601 ctaaacgatc aatataagga ctttgagcaa tagtagcatc atgagtaaag agtaagatac
     3661 cttgattttc ttcatgagct aaataagaaa gcaagtggag tagctcttgg atctccaata
     3721 cactaagttc atcaaatgta tcttcaataa caatattgtt tcgcttggaa agcaaggcat
     3781 gaagtaattg cagctttact ttttcaaaag acgtcaactc agctgccgca caattaagca
     3841 aggaaggaga gatatcaagt ttttcttgat aggccaaacg atcttgtttg atttcttttt
     3901 ccggtacatc caacaggaca tggtctttaa gattcagata aggaaacatc ggccaatttt
     3961 tttggatgat cgcaaaatta tccagttttg caacaagaac ctcttttttc aagtgctcca
     4021 atattttatg ctgtttttcg agaggacaca aaataacggt caagtgttca gcatgaatca
     4081 aatattcagg tttcattacg tccaggcctc ctactttgca aatgatggat aaaaaagtga
     4141 aagggaatag actaagaatg agacagcatc cactgagccc aataagagtt tgggcaaatc
     4201 ccttaagtat atctgtgaaa aaaagatcat tttggaaatc gatggtaaaa aaagtctgtt
     4261 gatccatcgg aattgtaagt ccgcttaccg aagtcaagat atcacggata tctggcagtt
     4321 cagtagtctt taagacaaga aaattgatcc tttgaagaag cgcttcataa actgactgga
     4381 agcttacgat ccacaataaa accaagatac aacaaagaaa gagaggaaag aacagctgcc
     4441 agaaaataaa aaggaataat ttggttttag aaagaccagt caggtgccaa ttgatcaatt
     4501 cctgcttgtt ttgcctgact gaatagtaaa agaaaaagat aaaaaggaag gcaaatagaa
     4561 aaaataaact gaatatgcct tttatataaa atcggctagc taattctaaa gccggaagaa
     4621 gctctgtaga tgcagaagcg ccaataaatg aaagccgatc ttggacttga ttggctaaag
     4681 ccgattgcaa atggtaaaga gtcaggcatg ctatcaataa aaaagcagaa aagcttgaaa
     4741 ataaacaata gagtattgtc tgctttttgt aataaattaa tcgagcagta gcatagcgga
     4801 ttattttcat acttttcacc tctataaaaa gtatagaaaa tgaatatgaa taaagtatga
     4861 aattccttta gtgaaatcac gaattaaaac cagaaaatat tttgtaaaaa gcaagaggtt
     4921 gtgaagaagc tgccctattc tattaccgta gatgcaagct tttgaacact gtttattcga
     4981 aaagaggagc agcgacaata atttt
//
LOCUS       DAZKNH010000132         4928 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000132 DAZKNH010000000
VERSION     DAZKNH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.134"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..970
                     /locus_tag="ACG8MG_002497"
     CDS             83..970
                     /locus_tag="ACG8MG_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0376489.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="ACG8MG_002498"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="ACG8MG_002498"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFQ0376490.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="ACG8MG_002499"
     CDS             2272..2718
                     /locus_tag="ACG8MG_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0376491.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="ACG8MG_002500"
     CDS             2991..4343
                     /locus_tag="ACG8MG_002500"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFQ0376492.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="ACG8MG_002501"
     CDS             4514..4801
                     /locus_tag="ACG8MG_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0376493.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1693 a          813 c         1030 g         1392 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttt
//
LOCUS       DAZKNH010000133         4731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000133 DAZKNH010000000
VERSION     DAZKNH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.136"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(126..488)
                     /locus_tag="ACG8MG_002502"
     CDS             complement(126..488)
                     /locus_tag="ACG8MG_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376494.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(562..942)
                     /locus_tag="ACG8MG_002503"
     CDS             complement(562..942)
                     /locus_tag="ACG8MG_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFQ0376495.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1024..2199
                     /locus_tag="ACG8MG_002504"
     CDS             1024..2199
                     /locus_tag="ACG8MG_002504"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFQ0376496.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2276..2860)
                     /locus_tag="ACG8MG_002505"
     CDS             complement(2276..2860)
                     /locus_tag="ACG8MG_002505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0376497.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3061..4059
                     /locus_tag="ACG8MG_002506"
     CDS             3061..4059
                     /locus_tag="ACG8MG_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0376498.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4171..4680
                     /locus_tag="ACG8MG_002507"
     CDS             4171..4680
                     /locus_tag="ACG8MG_002507"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFQ0376499.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
BASE COUNT         1502 a          890 c          835 g         1504 t
ORIGIN      
        1 attttgaaaa taaaatgcac taaaaaacta tctttagcag aacatttcat tgtatttttt
       61 ggaccaaaca ataaaaaaag aagtttgaaa aaaattcaaa cttctttgtt cgcttaatct
      121 gtattttaga agaaaggcac tccctctact tcgtaagttc caagaacaga gcgcatttga
      181 cctggttctt cccaaatata tccttcgcct aattcttctc tttgattctt ttgttcagtt
      241 tcaaagacaa acaacggaac attttcctct ttaatatgtg tatttgtcaa ttccatcaaa
      301 ctaatcttgc taacatcgat atgaactatt tcttttaaga aattaagtat attcgctaaa
      361 ccagtctttc cttcagaaag ttccgctaac gcaaattcca acttgtcatc aattgaatgg
      421 accaaaaatt ttttagtacc atcctccaaa tgcaacataa ttgtaccagc aactcttttg
      481 cttgccaaaa tttccacctc gatcaacttg ttctatggct tacataaaaa ttctagcaca
      541 ttttcagaaa aaatacactt tttatttctt attttctttc cgttctagtt cagataatgc
      601 ttcttctatc caaataggta actgctccgc aatagtggta aatccgatct tacgattttt
      661 attcgtgaaa tacctttttc tgcgatatgg aacagctgga tttgtttttt ctaacgttct
      721 taaagataaa ctgatttttt tggaatattc atcaatgtcg atgatctgaa ctgttactgg
      781 ctgtccgact tttaacaatg aatggatatt tttcgtataa ccggattgta cttctgaaac
      841 atgaataagt ccttgcgtct cgttatcaag agaaacgaat gcgccataag gctgtatccc
      901 tgtcactttt ccttcaatta tttgtccaat tttataagtc atctacttcc ctctcttcgc
      961 atttatcatt atactgtaaa tagctcaaaa aaagaagcga cgaaaatgac tggaggtctt
     1021 aagatgatcg aatttgataa acagatttca agaaaagata cagaaagtgt aaaatgggac
     1081 gcaattgctg agacatatca aatggatgac ttacttcctt tgtgggtagc agatatggac
     1141 ttcttgtctc ccggtggggt agctaaagcg tttagtgaat acatcaaaca tggaatcttt
     1201 ggttatgcca ctctgtctga taaactatat caggctatca ttcattggga acgccaacac
     1261 catgctgtgg agttgacgaa agaaaatatc gtttttacaa gtggtgtgtt aaccagttta
     1321 gctgcagcag tacaaacttt tacaaaacca ggcgattcta ttttgattca tgacccagtt
     1381 tatcctccat tttcctctat tatagaaaca aatcaacgtc acatcgttcg aagctctttg
     1441 ctagaaaaaa ataatcattt catcatggac ttgtctgaca tggagaaaaa aatcgaagaa
     1501 aacaatgtaa aagcaatgat tttatgcaat cctcacaatc ctggagggcg tgtttggagc
     1561 aaagaagaat tgaaacagtt aagcgaattg tgtttaaaac atcaagtgat tctgtttagt
     1621 gacgagatcc atcaagattt aactttattc gatcatacat ttacttccat gctaacgatt
     1681 gatcctaatt tagatgatct actagttgct tttacttccg caaccaagac tttcaatcta
     1741 gctgcaatca aaaattcaat ggtatttatc aaaaatccag agatgaaagc tgattttgaa
     1801 aaacatttag tgatgaacca acagcacgaa atcaacactt ttggactgat tggaacccaa
     1861 gcggcttatg agacgggtgg agaatggtta agccaactga ttccttatct agaaaaaaat
     1921 gttcaaacag ttaatcagtt ttttgaacag tatcttccga aagtgagaat aatgaaccct
     1981 gaaggaactt acttaatgtg gttagatttt accgcctata tttcagacga ccaagaattg
     2041 gagaacactc ttgttcggaa aggaaaagtc gtattaaatc ctggcatcac gtttggtcct
     2101 tccggtcatg ggcatatgcg cttgaatatt gcttgtccag aagaaacttt actggaagga
     2161 cttaatcgaa tcaagtatgc tttagagaca atcgattaaa tcatttattc aaaataaaag
     2221 gacgagacct tactaggttt cgtccttttt cataattact ccgtaccata ccaaactatt
     2281 ctgaaatttc aattgtttca atcactacat catgtacagg tcggtcttgt ggaccacgtt
     2341 gtacatttgc gatttcatct actgtgtcca tcccttctag tacatgtcca aagactgtat
     2401 gtcggaaatc tagccaagga gttccgccac ctttatacgc ttcaatgatt tctgatggga
     2461 atccagcacc ttctagttgt ccaagcatat tagctggcac atttgtgtta tttacaataa
     2521 agaattggct tccgtttgtg tttggacctg aatttgccat agataaagct ccgcgaaggt
     2581 tgaacagttc acgactgaat tcatcttcaa aactttcgcc ataaatactt tcgccaccca
     2641 ttcctgttcc agttggatcg ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa
     2701 tcacgccatc ataataacct tttttggaaa gttcaatgaa attttgaacc gtcttcggtg
     2761 ctagttcagg gaataattga acagtaatgt caccacggtt tgttttgata acagcttttg
     2821 gtcctttttc actttttaaa tctagttgtg ggaatgccat attgtttcct cctaatattt
     2881 gcatacttca taataataat aacataatac ctaacctctc gtaaaaatac tagatttgtt
     2941 ttcttttatt tttcatcact agatacattc tataacttac cgtttcgatt tcgaattcaa
     3001 acatttttga gaagcattct caattaaatt ctgctatagt gtgaaaaggg aggaattttt
     3061 atggaaatct atgacattac aatcatcggt gcaggtcctg tagggatgtt tgccgctttt
     3121 tacgcaggaa tgcgccaagc taaaacaaaa atcattgaca gtctacctca acttggtggt
     3181 caattagcaa ctttgtatcc cgaaaaatat atttacgata ttcctgggta tccagcaata
     3241 aaagcaagtg aattaatcga tcaattagaa aaacaattaa ctacttttaa ccatacattc
     3301 catttaaaag aagaagttct ctcgcttact cgtgaagacg aagttatcga gattactaca
     3361 aataaaggca tccattattc caaagcagtc attttagctt taggaaatgg ttcattccag
     3421 ccaagaaaat tgaatttaga taatgctgaa tcatttgaaa accacggatt ggattatttc
     3481 gtgaatgatt tgatgaagta tgctggaaaa aaagtcgcta ttgctggagg tggtgattct
     3541 gctatcgatt ggacgttgat gttagagcct attgcaagcg aagtttactt gattcatcgt
     3601 cgcccggaat ttcgtgcgca cgaacatagt gtatcacgct tgaaatcttc atctgtcaac
     3661 ttgcttactc cctatctcat cgatggatta tcaggcaata atggggaact gacagatatc
     3721 cggttaaaaa aagtgaaaag tgacgagacg attgatttga tgattgattc tctgatcgtc
     3781 aattatggat tctcttctaa tttagaacat ctttcttctt ggggacttga tagtaccaga
     3841 aatgccatta ctgtaaaatc agatatgtct acttctattc caggcgttta tgcagctggt
     3901 gacatctgta cctatgaagg aaaagtgaaa ctgattgcta ctgggctcgg tgaagcacct
     3961 actgcagtga acaacgcttt acactatatt aatccaaagg aacgaactca gcctggtcac
     4021 agcacaagct tatacgataa aaatcttcgt cctagttgat tttttatatc tctttgcatt
     4081 tcaagttaaa cccgaaagca gtttcttgca tttttttcat gttccgttaa catgaaatag
     4141 agaaagaaaa aataaagggg gagcaataat atggtcgtca aaactgagac tttagaagaa
     4201 aaggcaagag aactattgaa ggaacgtggc gtaactgtag aagatatcgc acaacttgtc
     4261 atgtttttac aaaaagacta tatttctgat ttgactttgg cggattgtac tgaaagtgtc
     4321 aacagcgtgc taacaaagcg tgaagtacat aacgccatta tcactggaat ccaattagat
     4381 atattagcag aaaagaaaaa attgatgagt ccgttgcaac atattatcga agatgacgaa
     4441 ggcctttacg gtatcgatga gatcctagcc ttgtcaatcg tgaatgttta cggtacgatc
     4501 ggctttacga attatggtta tatcgacaag gtgaaacctg gtattttaaa agaactaaac
     4561 agccacgatg gcgttcatgt ccataccttc ttagatgata tcgtcggagc gatcgccgct
     4621 gctgctgcca gtcgattagc acacaaagaa ccagaaaaaa gaagtcgcct tactcaataa
     4681 ttcaacaaaa aaagaaagaa aacgtctatt tagacgagtt ctttcttttt t
//
LOCUS       DAZKNH010000134         4720 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000134 DAZKNH010000000
VERSION     DAZKNH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.137"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..744
                     /gene="vanR-A"
                     /locus_tag="ACG8MG_002508"
     CDS             49..744
                     /gene="vanR-A"
                     /locus_tag="ACG8MG_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0376500.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            722..1876
                     /locus_tag="ACG8MG_002509"
     CDS             722..1876
                     /locus_tag="ACG8MG_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0376501.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2091..3059
                     /locus_tag="ACG8MG_002510"
     CDS             2091..3059
                     /locus_tag="ACG8MG_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0376502.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3052..4083
                     /gene="vanA"
                     /locus_tag="ACG8MG_002511"
     CDS             3052..4083
                     /gene="vanA"
                     /locus_tag="ACG8MG_002511"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0376503.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4089..4697
                     /locus_tag="ACG8MG_002512"
     CDS             4089..4697
                     /locus_tag="ACG8MG_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0376504.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1455 a          907 c         1109 g         1249 t
ORIGIN      
        1 taatctgata aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa
       61 atacttattg tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac
      121 gagaattata cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag
      181 tctgagattg accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc
      241 tgtcaaaaaa taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca
      301 gaggtagata aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt
      361 cgcccactgg agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt
      421 ggagtaaagg agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac
      481 acccatgagt gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata
      541 ctgcgaatcc tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag
      601 atatggggcg acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat
      661 ttgcgcgaaa aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg
      721 gttggttata aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta
      781 catgtatatc gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat
      841 gatccgaggg aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa
      901 tcacctggac gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat
      961 ttatgtggcg attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc
     1021 aaaatacttt gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca
     1081 aattgagctt tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac
     1141 tctggaaaag cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat
     1201 gtacttggcg cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct
     1261 tgacgaggct ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga
     1321 caaagcgtat cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct
     1381 acaaacgata acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac
     1441 cgatgaattt tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga
     1501 ggatctgacc gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa
     1561 aaacgccgct gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg
     1621 ggatgtggtg tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc
     1681 catatttgaa aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg
     1741 acttggattg gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga
     1801 aagcaatgat aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga
     1861 taaaaggagg tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac
     1921 tttttcttag gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact
     1981 taataccgta agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt
     2041 ggcatccccg ttttatttgg tgcctttcac agaaagggtt ggtcttaatt atgaataaca
     2101 tcggcattac tgtttatgga tgtgagcagg atgaggcaga tgcattccat gctctttcgc
     2161 ctcgctttgg cgttatggca acgataatta acgccaacgt gtcggaatcc aacgccaaat
     2221 ccgcgccttt caatcaatgt atcagtgtgg gacataaatc agagatttcc gcctctattc
     2281 ttcttgcgct gaagagagcc ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc
     2341 atatagatac aactgctgct aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc
     2401 cggatagcgt tgccgattat actatgatgc taattcttat ggcagtacgc aacgtaaaat
     2461 cgattgtgcg ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac
     2521 tcagcgacat gacagttggt gtggtgggaa cgggccagat aggcaaagcg gttattgagc
     2581 ggctgcgagg atttggatgt aaagtgttgg cttatagtcg cagccgaagt atagaggtaa
     2641 actatgtacc gtttgatgag ttgctgcaaa atagcgatat cgttacgctt catgtgccgc
     2701 tcaatacgga tacgcactat attatcagcc acgaacaaat acagagaatg aagcaaggag
     2761 catttcttat caatactggg cgcggtccac ttgtagatac ctatgagttg gttaaagcat
     2821 tagaaaacgg gaaactgggc ggtgccgcat tggatgtatt ggaaggagag gaagagtttt
     2881 tctactctga ttgcacccaa aaaccaattg ataatcaatt tttacttaaa cttcaaagaa
     2941 tgcctaacgt gataatcaca ccgcatacgg cctattatac cgagcaagcg ttgcgtgata
     3001 ccgttgaaaa aaccattaaa aactgtttgg attttgaaag gagacaggag catgaataga
     3061 ataaaagttg caatactgtt tgggggttgc tcagaggagc atgacgtatc ggtaaaatct
     3121 gcaatagaga tagccgctaa cattaataaa gaaaaatacg agccgttata cattggaatt
     3181 acgaaatctg gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat
     3241 tgctattcag ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac
     3301 catgaatatg aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt
     3361 gaagatggat ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat
     3421 attcaaagct cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct
     3481 gggatagcta ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg
     3541 tttacctatc ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa
     3601 gtcaatagcg cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa
     3661 atcttaattg agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt
     3721 gccgcgttag ttgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt
     3781 catcaggaag tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac
     3841 ctttcagcag aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc
     3901 ggctgtagag gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg
     3961 aacgaagtca atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc
     4021 gctgcaggta ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg
     4081 tgataagcat ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg
     4141 acgctaaata tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa
     4201 atcgcattgt agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg
     4261 ctacccaagg gtacggattg cttctatggg acggttaccg tcctaagcgt gctgtaaact
     4321 gttttatgca atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca
     4381 atattgaccg aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc
     4441 gcggcagtgc cattgatctt acgctttatc gattagacac gggtgagctt gtaccaatgg
     4501 ggagccgatt tgattttatg gatgaacgct ctcatcatgc ggcaaatgga atatcatgca
     4561 atgaagcgca aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg tttgaagcat
     4621 atagcctcga atggtggcac tatgtattaa gagacgaacc ataccccaat agctattttg
     4681 atttccccgt taaataaact tttaaccgtt gcacggacaa
//
LOCUS       DAZKNH010000135         4699 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000135 DAZKNH010000000
VERSION     DAZKNH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.138"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..1336)
                     /locus_tag="ACG8MG_002513"
     CDS             complement(104..1336)
                     /locus_tag="ACG8MG_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFQ0376505.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="ACG8MG_002514"
     CDS             1591..2160
                     /locus_tag="ACG8MG_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0376506.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="ACG8MG_002515"
     CDS             complement(2338..2778)
                     /locus_tag="ACG8MG_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFQ0376507.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="ACG8MG_002516"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="ACG8MG_002516"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFQ0376508.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="ACG8MG_002517"
     CDS             3732..4655
                     /locus_tag="ACG8MG_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFQ0376509.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
BASE COUNT         1425 a          867 c          927 g         1480 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataata
//
LOCUS       DAZKNH010000136         4633 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000136 DAZKNH010000000
VERSION     DAZKNH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.140"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            174..518
                     /locus_tag="ACG8MG_002518"
     CDS             174..518
                     /locus_tag="ACG8MG_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFQ0376510.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="ACG8MG_002519"
     CDS             519..938
                     /locus_tag="ACG8MG_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFQ0376511.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="ACG8MG_002520"
     CDS             complement(1030..1380)
                     /locus_tag="ACG8MG_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376512.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2677)
                     /locus_tag="ACG8MG_002521"
     CDS             complement(1346..2677)
                     /locus_tag="ACG8MG_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFQ0376513.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2933..3499
                     /locus_tag="ACG8MG_002522"
     CDS             2933..3499
                     /locus_tag="ACG8MG_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0376514.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="ACG8MG_002523"
     tRNA            3644..3731
                     /locus_tag="ACG8MG_002523"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            4065..>4633
                     /locus_tag="ACG8MG_002524"
     CDS             4065..>4633
                     /locus_tag="ACG8MG_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFQ0376515.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1683 a          778 c          891 g         1281 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatcaac gattagaggt tgtgaagaag ctgtcctgca tcaagtaata
     3781 agaacggcca aacaaaaata gcttttccta ttttttcgtt tgattgggct tattaccgca
     3841 gatgcaagct tttgaacacc gtttgttcgg aataaggggc tgtgacaaga cttttgtcac
     3901 agccccttat tgttgtactt ttaacctaca atattttttg attccccctg agtactattt
     3961 tttataaatg ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa aatactagat
     4021 gtaagataaa agtgtaaaaa cgatttgttt taggagggga aacgatgtgt accgcaatca
     4081 cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg
     4141 aagtggtcac tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag
     4201 ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca ctctattatg
     4261 aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt
     4321 ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac
     4381 tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca
     4441 atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag
     4501 aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg
     4561 gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac aattatcgct
     4621 cgctatcaaa tgg
//
LOCUS       DAZKNH010000137         4468 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000137 DAZKNH010000000
VERSION     DAZKNH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.141"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACG8MG_002525"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACG8MG_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFQ0376516.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="ACG8MG_002526"
     CDS             complement(2944..3378)
                     /locus_tag="ACG8MG_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376517.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..>4468)
                     /locus_tag="ACG8MG_002527"
     CDS             complement(3395..>4468)
                     /locus_tag="ACG8MG_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0376518.1"
                     /translation="YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRAN
                     NIDKVFFKLKMNNLNVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLF
                     HEPLIEYTENLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVF
                     NLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAV
                     SLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSV
                     SETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVF
                     KKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT         1384 a          826 c          614 g         1644 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4381 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4441 taatattatc agttagtaac tttttata
//
LOCUS       DAZKNH010000138         4335 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000138 DAZKNH010000000
VERSION     DAZKNH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.142"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..949)
                     /locus_tag="ACG8MG_002528"
     CDS             complement(53..949)
                     /locus_tag="ACG8MG_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0376519.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(942..1622)
                     /locus_tag="ACG8MG_002529"
     CDS             complement(942..1622)
                     /locus_tag="ACG8MG_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0376520.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1623..2405)
                     /locus_tag="ACG8MG_002530"
     CDS             complement(1623..2405)
                     /locus_tag="ACG8MG_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0376521.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2398..3315)
                     /locus_tag="ACG8MG_002531"
     CDS             complement(2398..3315)
                     /locus_tag="ACG8MG_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0376522.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3479..3733)
                     /locus_tag="ACG8MG_002532"
     CDS             complement(3479..3733)
                     /locus_tag="ACG8MG_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376523.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         1274 a          946 c          756 g         1359 t
ORIGIN      
        1 aatatatata agataataca tttttctact agttctcact aatcttgtat aattaaaccc
       61 tccgcaactt gacggagatg gagaagatgc ccccgtctaa tctagcggtc atctctcctc
      121 ccattccttc caccaatttt tttgacaggt aaagcccgat tccgctagat tccgtatttg
      181 tgcggctggt attttcggta taaaaacgct ggaagacttt ttcaacagag gtttgcgttc
      241 ctggtttgat aggattggcc actgttaatc gaatgaatgg atcctctttt gaaaatgtca
      301 cctttgcttg tccatctgca taacgcaata aattctgtat gatattttgc agcactcgca
      361 gcaacatgag cggatctgct aatacagttg tatttatcga gtgttcagcg aacgctacct
      421 cgatattttt ctcagaaaat tgttcataaa aagagaaaaa tacttcttcc acctctttag
      481 aaaaagaaag cgtatggaga tccatttctt tttgcttcgt ctctattcga gcaagatcat
      541 aaaactgatc cgtcatactg agtaatcttg caacagattg tttgatgatc gttaaatact
      601 tctcccgttt ctccgtatcc tctacctgtt ccagtaattg tacataaccg ttaatagcag
      661 tcaaaggagt tctgagatca tgagaaaggc cgacgatcga tagtctgatg ttttcttcca
      721 tttcttgaaa tgcttgtcct ttttgttcat tttcatccaa ccatgtattc agtgcatcta
      781 ctaggtccat tagattcttt tctcgaaatt cgactgaaag acgaccacca tattcttgtc
      841 gttcagaaag agtggcgatt ttcttagtta atctcttgat ttccctttgc aggaagaaaa
      901 taaaacttcc cataaagata acaacgagaa gcaacagcca tttaatcaaa tctaaacccc
      961 actccccaca ccgtatgaat atacacagca tgtgcctttg ctagcttccc tcgaagattg
     1021 gaaatatgca tattcactgt tttctcacta tcaaaatatg gttcttgcca aactgtttca
     1081 taaagatttg ctcgcgaaaa tacttttttc ggatgttcta aaaaaagctt cagcagatcg
     1141 aattctcttc ctgtcaaatg gacagccaca ccattcacat ttacatccct cgactctttg
     1201 tccagtaaga tttccttgta atagatgcgt tgcgacagat cttttacttg gacagaagtc
     1261 tgatgacgaa gttggatgcc gattcgtaac aaaagttctt cgatatcaaa tggtttcgtc
     1321 acatagtcat ccgctcctgc tgccaacaag gaaactttat ctctttgatc gctttttgct
     1381 gaaagaacga tgatcggaac attcgatgtc tgtcggattt ctgctaacaa tgcttcacct
     1441 gaaagccctg gaagcatgag atctagaagg atcaaatcga caggatatgt gcttaacaat
     1501 aattttcctt ccgtacctga ataagcatca agaagtttgt atctctgttc aagaacctcc
     1561 tccagtaagg aatgaacagc ttcatcgtct tcgataatca aaatagtttc cagcaggacc
     1621 acctacttaa tgtcagattt ttggaaaata aacattccta gagctgatga taccacgatt
     1681 gttactagac tgaccagaat cattttcggt gcttgcgaat aagttgcttg ttgcgcaaag
     1741 tccgtataca tgagccacgt ttccgtaaac cactccgtta gaaattccaa gcctttgatt
     1801 tttgcgaaaa caccgggaat gaaggccgtt cccatgatat aaagaaaaga aagaagcaaa
     1861 ctgacactgg tacttctagt gatgaatgtc aaagccactg gaacagataa tgtccctaat
     1921 gtcagcagaa gcaatacagg aaatactttt agaacataat ttccatagtt tttgattccc
     1981 tcaacttcac caaatccgaa agcaagtgtg taagcaagca tggctagtcc cgtgatcaaa
     2041 accacgccaa gtgttgtgat caaaagcgta acgagaagtt tcgacacata ataagtcatt
     2101 cgattccgtc ctaaagataa cgtattgcga atcgtaccgt tttgaaattc ttccccaata
     2161 taaaagcacg caaatgctgc gaaagccaga agaatccatg cagccatttt ttctaccatt
     2221 aaaatccctg ttgcaccgtt gatgactgta tccgttgctt ggtttaattc attgccgtat
     2281 tttgtatgca tataccattc gttccaagaa taaaaaagcg gaagtacgac catcagtcct
     2341 agtgagaccc acattttacg gctcttccat acttttattt tttcagcagt taatagatta
     2401 aacatgtgga ttgcctccag ttaattgtaa gaaataatct tccagttttt gcccaactag
     2461 gtgaatacca tccacaggaa cttttgcttc taccaatgct tgattgatgg ctgctacttg
     2521 gtcaattcct tcaaaaagct ggataacacg gctagattgt acaaaataat ccctataacc
     2581 aagagagtcg agaaccgtta ctgcttgttc cgtcgccgaa gtgtcaattt ttataaattg
     2641 acgagattct tgtgccaatt cttctttcga taattctttg atcaagtgtc cttcatgcaa
     2701 aataccatag tgagtggcga tctgtgacaa ttcatctagc aagtggctag aaagcaagac
     2761 ggtgatgccc cgttctttta ccaaacgttg gatgatttct cgcatttctg ccatccctgc
     2821 tggatccatc ccattcgtcg gttcatccaa aatcaaaaat tctggattcg tgatcagtgc
     2881 atttgcaata gctaaacgct ggcgcatacc taaagagaaa ttctttgctt tctttttccc
     2941 tgtattttcc aatcgcatca ttttcagcag gtcttcgatt tctctatccg acacaccgcc
     3001 gttcgctgct tggatacgta ggttatctct tgcagttaat tctggataaa gagcaggggt
     3061 ttcaatcgat tgacccatac gtcgtcttgc tgcctgcaaa tccgtcgttc caaataattc
     3121 gatcgaacct tcatcaatcg agatcaaacc cataatagtt ctcataaagg tagacttacc
     3181 cgctccattt tccccaatca atccatagat cattccacgt ttgatttcga ttgatacttt
     3241 atctaatgct ttggcagcac cgtatttttt ggttatacca gttgctttca agaccgtttc
     3301 atttccaaaa tccatactta tccttctttc ttattgatga gtgaagtata gttgacgggt
     3361 ataaagaaag cagccagaaa tgtataaaga aagtgtaaat tttccaattg aatgtcaaaa
     3421 aaaacaatcg gacaatacgt gtctttccta ttatcaggca gtttgttata cgcttgtatc
     3481 agtttcctga ttctttcttt tcgaccaaag tcgtttctct gtattcctct tcactgaaaa
     3541 tttttgcttc tttcagatca agcttgagta attttaccag accggttata aggataacta
     3601 tagcaagact ccctcccaga aaaattagac tgtctcgcag acgtggttgc gttgtcttcc
     3661 aatcaaagag cgcaccaaac agtgctaatc caagtggact ggccaccatg gtaatcgttg
     3721 ttatcgttga cataagtcgt ccttgtatat gcgttggaac ttctcgctga agccagacca
     3781 tataaaaagc aaaagaaaac attaatcctt aaaaacggac ttatgaaaaa taatacatgc
     3841 cgtttgggat ttacaattag ctaccaaata agaggttatg acaaaagact tttcacaatc
     3901 ccttcttccg aataaacggt gttctcctgc caactcctcg gtcacaagtc acaatattta
     3961 tcaaacatga gaagcaattt ttgtaaattc cttcttattt cttgaagctg accacttctg
     4021 tcacaacctc agaagcggat aaataatatt gtttgatgat actttatctt tttcccgttt
     4081 gtttatagat cgaatgatat tatagaccaa gaatcctacc tattaacata gctaaaaaca
     4141 caaaaaaggc actttccaaa ttgtggaaag tgccagaaaa gttaggatta aggtaactgc
     4201 gctcagcatt gcttcgccca gtacgattct cgtaactcgt gaactcgaac gataatcgca
     4261 attaacgttt tgagaactgt gaagctttac gggctttctt aagacctggt tttttcttcg
     4321 tatcattgaa taagt
//
LOCUS       DAZKNH010000139         4310 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000139 DAZKNH010000000
VERSION     DAZKNH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.143"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..1285)
                     /locus_tag="ACG8MG_002533"
     CDS             complement(65..1285)
                     /locus_tag="ACG8MG_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0376524.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1916)
                     /locus_tag="ACG8MG_002534"
     CDS             complement(1305..1916)
                     /locus_tag="ACG8MG_002534"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0376525.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="ACG8MG_002535"
     CDS             complement(1959..2891)
                     /locus_tag="ACG8MG_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0376526.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="ACG8MG_002536"
     CDS             complement(3312..4172)
                     /locus_tag="ACG8MG_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0376527.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1230 a          866 c          707 g         1507 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct
//
LOCUS       DAZKNH010000140         4295 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000140 DAZKNH010000000
VERSION     DAZKNH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.144"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..792
                     /locus_tag="ACG8MG_002537"
     CDS             <1..792
                     /locus_tag="ACG8MG_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0376528.1"
                     /translation="FCGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKK
                     GQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSF
                     KGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEI
                     IRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEY
                     SIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            889..3138
                     /locus_tag="ACG8MG_002538"
     CDS             889..3138
                     /locus_tag="ACG8MG_002538"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0376529.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3141..>4295
                     /locus_tag="ACG8MG_002539"
     CDS             3141..>4295
                     /locus_tag="ACG8MG_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0376530.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1501 a          610 c          790 g         1394 t
ORIGIN      
        1 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
       61 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      121 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      181 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      241 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      301 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      361 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      421 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      481 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      541 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      601 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      661 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      721 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      781 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      841 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
      901 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
      961 ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata ttatggtgag
     1021 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1081 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1141 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1201 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1261 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1321 gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga ttatcctgtt
     1381 attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat cgagaatatt
     1441 agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt atctggtgct
     1501 tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca atcaatcgaa
     1561 tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa aagaaaaaat
     1621 actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg aaacttctta
     1681 attcaagcag aagtagatac atgggatgtt actagactac aagttggcat caatccattt
     1741 ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc gattctggtt
     1801 tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt tagaaaacgc
     1861 ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa ttgggaagca
     1921 acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc atttgatgtt
     1981 ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa cgattgtgca
     2041 ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg cagcctttca
     2101 gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga aatggtgaat
     2161 aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc taatcgaaaa
     2221 cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt tgttgaaaat
     2281 attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat taagtgggat
     2341 atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca acaaggagaa
     2401 gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat aacaaaatat
     2461 cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc tggaatgctt
     2521 cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag gttaaaaatt
     2581 caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt atcaattgtt
     2641 ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt agcttttttt
     2701 ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt gaggattgta
     2761 aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg tactttttat
     2821 agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt tagtcatgat
     2881 aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc tccttaccgc
     2941 cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca aaatagtttg
     3001 cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc tagtggacaa
     3061 ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat ttgggttata
     3121 acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa ggagcaatct
     3181 taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt ccacaaatga
     3241 aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca ccaagtataa
     3301 ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga atgaaaaaga
     3361 caataatttt aggtttatta ttagctggta taggaggaat tattccagta tttacagcta
     3421 ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga gtatataatt
     3481 cattggctgt gacatatatc agctctttat actctggcaa tactagagca acgctattag
     3541 gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt gctggagttt
     3601 tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt cctgtagctc
     3661 tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata cctgaaaatc
     3721 atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca atcttactcg
     3781 taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa attaaagggg
     3841 taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata ctagcaggat
     3901 ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg ggaattatat
     3961 tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg ttcctgctaa
     4021 cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc tttagtaact
     4081 tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt tttattggct
     4141 gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt ctaacaagaa
     4201 gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg atcctatttg
     4261 taacattttt tgcgacaaat aaaaaaaata ctaga
//
LOCUS       DAZKNH010000141         4256 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000141 DAZKNH010000000
VERSION     DAZKNH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.145"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..493
                     /locus_tag="ACG8MG_002540"
     CDS             56..493
                     /locus_tag="ACG8MG_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376531.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            588..1472
                     /locus_tag="ACG8MG_002541"
     CDS             588..1472
                     /locus_tag="ACG8MG_002541"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0376532.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1694..>4256
                     /locus_tag="ACG8MG_002542"
     CDS             1694..>4256
                     /locus_tag="ACG8MG_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFQ0376533.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
BASE COUNT         1394 a          713 c          960 g         1189 t
ORIGIN      
        1 tgtataatct taaataaaga aaacgatatt aaaaaatgca cgtagaagta gataaatgga
       61 gtggttttgc aaatttcttt tatttctaga agttattcac gtatacggat ttgtttttct
      121 cacaaataaa aaggcaggaa gtacaatgga tcatttgtat ataaaatata ttcatcaatt
      181 ttccgttttg gtattaggca cactaattat agcggggatt gttgcattta ttattcaagg
      241 tcgtgacata ttaacagtac atcaagagtt tttcagttat gcatttggta ttcctctgat
      301 cgctataagg tctgataaca aaaagtggaa gaaacgctat tacattctct tgctattttt
      361 gtgtttttat ctgccaccta agatttttac tttattaggt atacaatgcc tatcattttc
      421 ttttatttcc tatatcattg ttttaatttt cctcagtttc atagtatggg tattttatct
      481 tgtcaaaaaa taaatgaatg tttcatgaac agtacttcat cacagcctaa tttttttctt
      541 tatgctatcg ttatattaaa gaaatcaatc agaagggaaa gaaagtgatg aaaaaagttt
      601 tattagtggt aaaccccagt tcaggcggtg aacaagcaaa ggaatttgag caattagcga
      661 tagcaaaatt ggaatcggtg tttgatgaag tggtcgtctt gcatacaaaa aaagcagggg
      721 atgcaaaaaa ctttactcgc gaagcggcta cggaagggta tcatagtgtg tttgtcatgg
      781 gcggagatgg aacagtcaac gaaggaatca gtggaatcgc tgaacaagaa catcggccaa
      841 atttcggatt tttcccattg ggtacggtga atgacttggc tcgagcatta gggataccgc
      901 tagaacctga ggaagcgatc aatcattttt cgattgaatc agtcaagcca ttggatattg
      961 gaaaaataaa tgacgactat tttatgaatg tcgtcgcaat tggctctatc cctgaagcaa
     1021 tcaatgatgt agatgcggag aagaagacga aatttggaaa actagcttat ttcatgtcag
     1081 gaatcaaaca gttagcttcc acacaatcct actcgtttca cgtagaagtt gatggaaaga
     1141 aggaagaaat tgagagcagt atcttgctga taggactaac taactcagta ggcggttttg
     1201 aaacattact tccaaatgca aaagtggatg atggtaagct ccatttggtt tatttaaaag
     1261 attcttcttt gatcgatacg cttaaaacat tgccagacct attaaaagga gtagatgaat
     1321 ctacgaaaaa tctagcctat cttacttgtg agcaattagt tgtgtcatta gctgatgagg
     1381 aagaattgac gacgaatgtt gatggagatg aaggggataa attacctgtg acaatcaaag
     1441 tgttaccctc acatttacag gtttattgtt aaaatggagg ttgtgaccga agagttgtca
     1501 ggaggacatc ggttgttcgg aataagggac tgtgacaaga cttttgtcac agcctttttt
     1561 tgtacaaaaa agaaattttc acctaacaga aaaatttgcc ataaaaatac atattttaaa
     1621 tatgaaagta caattacaaa taaattaaaa cgttaaaata aaattgtcaa aacgtcagag
     1681 aggatggtgg agaatgaatc aatatgtaaa aaagctgatt gcgtcgattt tgtttgtctt
     1741 ccctctcttg atggtgacat ccgtttttgc cgatgtagag aaaacaagcg aatcaacgca
     1801 atcagagaat caaagttttg acaagactac tttttcagaa gaatcaatta cttctgaaac
     1861 tacagaatcc caaagtgagg cgcctacttc taatacaagg caagaagaaa ataagacaag
     1921 tgagacctca gaagtagctg aacaaacgtc tgatagcagt gaagaggtac aggttagagt
     1981 tgtttcagcg gatgggtaca gcgaagctgc cacaacagaa gaactgacgc agcttctcgc
     2041 agacgaatca gtagctaaga ttcgtttaac tcagcctttg actttagaca gggaattgga
     2101 gatcaagcga gatatcgtta tcgattttgg cggacttgct cataatttcg gcacacatca
     2161 catctatatc aatgagacac ctcagtcgat cgagtttcag aattttaaag gaacggcagc
     2221 tcatccaggt gggttgatac caaatatcga tggaaatgcc attatcattg cttatatgtc
     2281 cgatttttgg ggaaacagat atcattttac gggagaaata aaatttaccg ggacacttga
     2341 cttatatgat ggttctaaat taggcctgat ttacgcccct cgtgcaactg tcacattaga
     2401 tggtgtttcc ggtgtattgg atgtccaacc agtgaaagaa gggatgaatg ccgctccggg
     2461 taaagcttat ttttgtagaa gctatcagct aaatgtaatc aatggtagtc agctttatgg
     2521 accatatcta ggaaaattct atggttttct agattcaggt gatgagacgg gcagtggaaa
     2581 aacagcacca ggactccata tcttgagtgg ttcaaaggtc tctcttgact atgatcgggc
     2641 agatggcgtc agcgatgaag gagaggctgt cgatacgcta cccgggaatg tcacatttaa
     2701 agtcagtggc tcaggaagtg aattttcggt aaatacaaag atcaatatta ccaacgacaa
     2761 caatcgcgga attatccaaa tgagaggctc aggatcaaga gtcgctgtgt cgaatgatgg
     2821 gaaaatcaca atcaatacag cgactactgg cggatttcgc ctgcaaggag attctagtca
     2881 aatccatgta tcaagtggtg gacaaatctc ggtcaaaatg gctggagatg gcgaccaacc
     2941 tggaaacaat gggatgcgtt ttgtagggaa aggactttct ttgatcgtag aaggagccgg
     3001 atcagtcatc gatattgata agcaaagtgg tcgttctagc gcagttcgtt ttgagaacgg
     3061 tacgcaaaaa ctaacagtaa ctaatggagg ctcattgaag gtaagaaatg caggggatgg
     3121 caaatcttat gtgaacagac tcaatcaagg aaaccaagcg atccaatttt atgacgcttc
     3181 tggttttcta gaaagtccag ggtcagctga ttttacggtt actggagaaa aatcaaatat
     3241 cgatatacgc gcagattccg gagctaccgt agatacaact ggagatattg atttgaactt
     3301 tatggctggc gaaaaaactt atttagtcat ggtcgggaaa actggggaag ataagactgg
     3361 catcatcagt ggggatgtgt tgaatgtaga cttagaatct cctacctatt tcgatttcca
     3421 aaacaaacgg atcggaagcg agactaatgg cataggtggc tgggtatttc aaggaaatgg
     3481 cgattcttct ttgaagatcg ctcattcaga aattgcttta tggcgaaagg caggagtaac
     3541 gttcgataac tttgaaaaag atccggatta tttttctgat atgacagact tatcgatcaa
     3601 tggaaccgac ttaggtaatt ttgtatcaag ccctagtgca ggtttaacaa atgaatttgc
     3661 tcagacaggt gccggaatga aaaattataa tcgaatcagt gccaataacc agcttccgat
     3721 gattgaatcc ttaagagtac caacgaatgc agataaaaaa atctatgggc atgtcgttgt
     3781 tcccgaaggt gtcgaggcaa agccaagaga tgcttggaca ggcgaagtag aagtgacttt
     3841 acgtattact tatgcagata cgacgaaatc acctgtagaa attactgctc tcacacaagg
     3901 acaaacagat gaaaatgaag ggacaggata taatccgtgg ggagaaggcg aacaaggcgg
     3961 tttgtttgag gttcaagtac ccaacgaaga aagactaaca acgggggatc aagtgcaaat
     4021 agttgctgct aaaaaagtaa gaggcggcca agaagtgaca gaactggact ctgccaaaac
     4081 gacagtcgat gtgattcctc ctgagcctgc taaattaagt accacattga tcaatgcagc
     4141 ttctcgctca atcacaggta caggcgaagc aggagctttg gtttcattga aaaaaaatca
     4201 agctgttttg tccgaaacaa ctattgatga tacaggtaaa tttgttcttt ctatcc
//
LOCUS       DAZKNH010000142         4197 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000142 DAZKNH010000000
VERSION     DAZKNH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.146"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..562
                     /locus_tag="ACG8MG_002543"
     CDS             92..562
                     /locus_tag="ACG8MG_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFQ0376534.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            647..1255
                     /locus_tag="ACG8MG_002544"
     CDS             647..1255
                     /locus_tag="ACG8MG_002544"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFQ0376535.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            1389..1589
                     /locus_tag="ACG8MG_002545"
     CDS             1389..1589
                     /locus_tag="ACG8MG_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0376536.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            2343..3569
                     /locus_tag="ACG8MG_002546"
     CDS             2343..3569
                     /locus_tag="ACG8MG_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0376537.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(3677..4147)
                     /locus_tag="ACG8MG_002547"
     CDS             complement(3677..4147)
                     /locus_tag="ACG8MG_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFQ0376538.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         1484 a          623 c          830 g         1260 t
ORIGIN      
        1 atcaaaaaaa attaattttt ttaaataaag atgaaagtta ttgaaatata gagtatgata
       61 aataagaatt tttaataagg taggttggct catgaagcag tatcgacagt taaaaggata
      121 tttagaagca aaaggatatt gggagatact agtctatctc ttttttggtg gtttggctac
      181 gattgtaaat tttgttagtt ttgctctcgc acgccaatat tttgatttaa gtatggcctt
      241 atccaattct atttcgtggt tttgttctgt attgtttgct tttgtaacga acaaactttg
      301 ggtattccat tcaaaatcac cgaattttac acatgcactt attgaatgcg gtaagttctt
      361 cttctaccgt atcctttctt acggattgga catgggggca atggtattgt tgatcaatgt
      421 tatgaacagc aatgagtatg tggcgaaaat catcactcaa attattgtta tccttgcgaa
      481 ctatattttc agcaagttgt ttatttttaa agaaactgag gtttttgaag aagaagtagg
      541 tcaaagtgaa aaagacgact aggcataaca attgaagaat ttccttctct ttgttactct
      601 ttgtaatgta gacattatta agaaaacatt gaggaggaat ttttttatga cttacacatt
      661 accagatcta ccttatgcat atgacgcatt ggaaccttat attgacgaag aaacgatgca
      721 tctgcatcat gataagcatc acaatactta tgtgacgaat ttaaattcag caattgagaa
      781 atacccagaa ttaggcgaaa aaacaataga agaattatta tctgatatgg acgctattcc
      841 aacagatatc aagacagctg tacgtaacaa tggtggcgga catgctaacc attcattttt
      901 ctgggaaatc atggcaccaa atgctggtgg cgaacctaca ggagaaataa aagaagcgat
      961 taatgaagct tttggtgatt tttcttcttt taaagaagaa ttcaaaaaag cagccgctgg
     1021 acgatttggt tctggatggg cttggcttgt aatggaaaat ggaaaattag ctattacctc
     1081 tactgcaaat caagattctc cattgatgga aggaaagaca ccaattctag gtttggacgt
     1141 ttgggaacat gcttattatt tgaaatataa aaatgtccga ccagattata ttgcagcatt
     1201 ctggaatgtt attaattggg atgaagtaaa caaacgtttc gaagcagcaa aataaccatt
     1261 tcctccgtca tatcagtctt aaaaataatt tggaaaataa aagagcgaaa gcttgctagc
     1321 gttttctaaa ttgggtataa tgttcttgca aggttacttg caagaacttt tttggaggat
     1381 atacatttat ggaacacgga acggttaaat ggttcaacaa tgaaaaagga tttgggttta
     1441 ttacagtaga cggcggagat gacgtatttg ttcatttctc agcaattcaa ggagacggct
     1501 tcaagagcct agaagaaggt caagaagtag aattcacgat tgttgaaggt gcacgcggac
     1561 ctcaagctgc tgaagtgact aaattataat aatttgttga ataaagacac gacttcttag
     1621 agagtcgtgt ctttttgtct atttatgaag tttttagatt ttttggctaa gtaaatgcat
     1681 ttgttcactt gacatgtcct aactaattat caagcaagga acattttctt cgttagtaat
     1741 ggattttaaa gaaatttcag tttttggatg ttgaataaaa gaccatttgt gaaaccacta
     1801 gtatacatac tcattaccat gtcaattcta ttactttaaa agaaaatcgt tttactttga
     1861 agaggagcat atctatccat taaaagatga tggtaatgca atcactagtt cgagtcattt
     1921 taagagccat gcagtgagct ggaaataaaa cgaatagaag atgggtttaa caaaaggacc
     1981 agtcatagga agaaattgtc gtgaaatcga atgataatct gaacggtagg caaagactgt
     2041 taaaaagatg ggaaaggtat gtgcgatcat tttactgcac gattatttgg aaaatacctt
     2101 gttgttcgct ttttctggaa aaaatacata aaaaaagata aaacgattgc gaactgcgtg
     2161 tattcggtct ttcgatcttt ttcacggtat ggtatgatag ttaagaattt tgagaaattt
     2221 taaaaaataa taatcgttac acaaaaatgt tttttaaagt tttcttatga ttaaaaagtg
     2281 aatttgctga ttgaacagtt atatgagaca atattttaga aaatgtgaaa cagaggtgta
     2341 ttatgaagcg atggcaaaaa gttctattaa ccttgttagg ggtattcgtt ttgctgattg
     2401 ccggattatc agcatacgga atcagattta tgggagaagc gagccaaaca gtaaacaaaa
     2461 tttctaaaaa ctctaatcga atatcctcca aacgttctga gagagtaagc attgatgaca
     2521 aagaaccttt ttcggttttg ctattaggtt tagataccgg tggattagga agaacggaac
     2581 aaggacgttc tgatacgatg atggtagtaa cagttaatcc gaaacaaaaa aaatcaacga
     2641 tcatcagttt ggaccgagat atttatacga atatcgtagg ttatggaacg gtagataaat
     2701 taaatcatgc ctacgcattt ggtggtgtcg aaatggccat ggattcaatt gagcaactgc
     2761 tcgatattcc tattgatcat tatgtaacga tcaatttgga tggcatggaa gatttgatca
     2821 atgcggtcgg tggcatcaaa gtgaataata aaattgactt tactttggat ggtgtccatg
     2881 taccagtagg tgaacaaaca ttgaatggag aaaaaggttt agcctattct cgaatgcgac
     2941 acgaagatcc agaaggcgat atcggtcgac aacgtcgtca gcgggaagta gtgacgaaaa
     3001 tcgtcaataa agttttaagt ttagatggcg taagcaatta tagaaaaatt ttaaaagcag
     3061 ttgagaacaa tgtgacgacg gacttagatt gggatgacat gcttgatgta gcgacgaact
     3121 acaccccggc gtttgaaaca atcaaacaag accagttgca aggcgaagga caaatgatca
     3181 atgatatcta ctatcaaatt ttaggaaaaa atgatcttct ttcgatccaa aatgaactga
     3241 agaaacaatt aaatataaaa acaagcgata cattacctaa tttgaaaaat gagaatgctt
     3301 caatgatgtt ctttgatgat tcggaagatg gcgattcgac aaacggtgat ggatcgaata
     3361 gtgaagggta caccgattat tcttcctatt cagaaaacac atatgatcag taccaagatc
     3421 cgaataccta ttatcagcaa acagaagata caagtcagta ctatacacca agtcaagata
     3481 ctcaaagtta tgatccttac gctggacagg aacaaaatgg ttatggttat gaccagaata
     3541 atgagtatgg aacaacaagt gggtactaga aaaaaatagt ttggttaata aagagtgcca
     3601 cctaaattag acaaaaaatc taacttaggt ggcacttttt ctatgtgtta aattacagga
     3661 cgtctctttt tagcgcttag tttaaaaaat aaattgaata attcaataga gttgcgaata
     3721 gtacccataa ataataagga atcatgagcc acattgccag gcgatgatgc agccacaact
     3781 gatacataag cactgacaag aaaataacta aaagtgtaat atcgattgct gcaatgaaaa
     3841 tatttcttaa attaaaaaat agagcagacc atataaaatt aaaaatgaac tgaatcacaa
     3901 aaagaccgac aagattgttt cgctgttttt tgttgttgtg attaagtaac aaataaagag
     3961 aaatacccat caatatatac agtaaggtcc acatcggccc aaatatccag ctaggtggtg
     4021 caaatggtgg aagttgcaaa gaatagtaat attttccggg atttccgcta agcaaccctg
     4081 ataaaaagcc taatatgaca attcctataa cacaaaccca aaaacgaata tcttttatca
     4141 tcttcatttt actaactcct ttctataata ttagtttacc atgaaggctc acagcaa
//
LOCUS       DAZKNH010000143         4075 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000143 DAZKNH010000000
VERSION     DAZKNH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.147"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1730)
                     /locus_tag="ACG8MG_002548"
     CDS             complement(<1..1730)
                     /locus_tag="ACG8MG_002548"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0376539.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLS"
     gene            complement(1690..3444)
                     /locus_tag="ACG8MG_002549"
     CDS             complement(1690..3444)
                     /locus_tag="ACG8MG_002549"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0376540.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3554..>4075)
                     /locus_tag="ACG8MG_002550"
     CDS             complement(3554..>4075)
                     /locus_tag="ACG8MG_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFQ0376541.1"
                     /translation="LVIYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFVYISYNYPRK
                     GIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQVASWALMQ
                     LGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVASLSMIG
                     VLIGRKVLKKYKK"
BASE COUNT         1375 a          780 c          576 g         1344 t
ORIGIN      
        1 tcagatagtt tagttttctt tatgagattt ttgtatattt ctgaccttcg atacaactca
       61 ttatgagtac cttgatcaac tagttttcct tgatccatca ctacaatttt atttaccttt
      121 tcaatagact tcaagcgatg cgatataatt agtactgtct tatcttgtat taaacgattt
      181 aaactttcct gaatacgttt ttcattttcc gcatctaaac tggcagtaat ttcatctaaa
      241 attaaaattg gcgcattttt caaaaaagcc cgtgctatgg ataggcgttg tcgctctcct
      301 ccagaaagtg aacttccgtt ttctccgacc aatgtatgat aaccgtcagg tagtttttca
      361 ataaagtctt cgcagttagc taaacgagct gcttttttca cttcctcatc tgacgcttga
      421 ctatttccaa tacgtatatt ttccaaaata cttgtattaa acaaagttac ctcttgaaaa
      481 acaatagcga ttttgctaaa caaagaagct ggagatacac gtttaatatc atacccatca
      541 attaaaatac agccttcatc ataatcatac agtctagaca ctaatttcaa gatactagtt
      601 ttcccggaac cactagcacc tactaacgca gtcacttctc cttgtttagc cgtaaaagag
      661 atatgatcta aaattggggt attgttatca tatgagaaag agacatctcg caactcaatg
      721 tcaaaagatt tcagaggact atctgatccc tcttggatac tggtttcctt catagctcta
      781 attcgctgta ttttaggagc taaataaaat atttcaagca ctgcttcttt catagaatcg
      841 aaggaatcct ttatttttat tgcggctagt aagtaaccaa cgacgtagag tatagtcact
      901 tcacccgtta acagtaagtg aacacctact agaatgacta ctgctaaact aacatatgag
      961 aaaatagagg ataaggccat gactgaaaaa gtacttaatt ctacctttaa atgaatcctt
     1021 tcactctctt ccattttttt gaaaagtctg tcttgaactt ttttagatag attaaagcta
     1081 ttaatctctt gctgcaattc tatagtttct tgaaattctt ccgagttttc tctcaaagta
     1141 tcgtaatatt tagtattggc tttcgtttgg gatttctttg ataacaagat taagacaaaa
     1201 ctaaataacg ttggcaaaat aacagctaat cccattttga cattaccaac caaaagcatc
     1261 actgaaataa aagggaaaaa cagagccata ccgccggctt taggtattgc atgactcatc
     1321 gcatgctcaa taccttcaac gtcagacatg atagtttgag ataaatctga taaattatgt
     1381 tttgaaaaat aggataatgg taaatttgac aaatcgtctg caatttgcac tcttaaatgc
     1441 gcactttctt tataagtact attatataag ttttcatact ccctatctaa caaccaatac
     1501 atgaaaagca aagtaacaaa cgatacggca aaataaagcc aacggggctt tgtattttct
     1561 aaaactaatt cgtctatcag catcattata atgaatgctg gtaccatatt aatgcagtat
     1621 acaaaaaaac tagaaataga agctttagtt aaagcttctg cgccctgatc acttaaagca
     1681 aatttgtttt tataaaaact tttcattgtc aagcctccaa tcatttgcat tctcataatt
     1741 agtgaccaat tgtttataca cttgatgttt agccattaat tcattatgat ttccacgttc
     1801 tactattttc ccttctgaca agacaagaat ttcatctaca gatttaatgc tggataatcg
     1861 atgagctatc ataataactg ttttttcttt catcagatgt tgaaaagcct tttgtagttc
     1921 atactcatta tctggatcaa tagatgcact ggcttcatcc ataataacaa tctttgcatc
     1981 ttttaaaatt gctctggcaa tagctatacg ttgtttttct cctcctgaca gataaactcc
     2041 tttactacca attaaggtat gctctctttc gggaaatttt tctaaaatcg attcgcaacc
     2101 ggctaatctt agcgcgtcaa atactttact tgctggcgct tgaggatttg ctatagaaac
     2161 gttatcgtaa atactctgat tgaatagttt cgtatcttga aagacaaaag caattgtgtt
     2221 aataattgtg tcttttgaat agtgatttaa aggcttacca ccaattttaa tctgtccctt
     2281 atccacattg tagaaacctg aaattagttt agctatcgta ctctttccgc ttcctgattt
     2341 tcctactaat gcataaattc ttttttgttc taaaactaag ttgaaatttt ctaaaacaca
     2401 ctcttctttg tatgaaaagc tgacattttc aaatgcaatg tcacaatttt taatctgtgt
     2461 ctcgttccca tattttagtt ggtcttgctt catttctaaa taaagggagt ctagtgtttc
     2521 gactgcatat cttgcttcaa acaagtgcat acttacatac attattctca tgaaagaaac
     2581 aaaaataatt ccacttaaaa aaaacaacat caacaagtca acagctaata tcttgggagt
     2641 ggtaagagaa gttataaaaa aggttatagg aactatcaaa atgcaaacta tgccaaaaaa
     2701 tagccattga aaaagaacaa aaggtgtttt cccactcaaa gaatacttat atgcaaactt
     2761 agcataatcg ttaattgctt tattcaaggc tttaaatgat gttactttag caccaaatat
     2821 ctttataact tgaattccgc ggatatattc taccgtttca gagcttaaca catctaacga
     2881 ttcttgatat ttttgaataa aatttgtatt ctctgtcatt ttctttaaca agaatacacc
     2941 aattactaac atggcgatta gaaccaatcc aattttaaag ttaatgaaaa agcctaaggc
     3001 aactgataaa acaggtgtta ctatggcttg agcactatct ggaatcaagt gagcaattgc
     3061 catatgtgtt ttagccgcgt tgtcatcaat tgtctttcga atatatcccg aagaatttaa
     3121 atcaaaaaaa cgaaaacttg cttctgttaa accgctaatt cccttttttc tcaaattagt
     3181 ctctaagcga aacccaagga tatgagaaaa taagccagaa aaaatataaa ataaagctcc
     3241 taaagttaac caagcaacag tttgaaccgc ataactctta gctaaatatt catctcctac
     3301 aataattaga gactgaagaa atttgtaaat actgtaatat ccatagactg ttaatacgac
     3361 agaaaaatca gaaaataaag tggctaacca tccgtagtat ttcattttag ggacataact
     3421 aaataatttt ttgtatactt tcattttttc ctcccccgca ttcaatttct tctcttattc
     3481 gtttacaact gttaatgctt tccgtaatag cgtaaattag agatgtacaa acagataaaa
     3541 ggtaaactac gttctatttt ttgtattttt ttaagacttt tcttccaatc aatacaccaa
     3601 tcatagataa acttgctaca actaacactg aaatgattcc ccatatacct accgcatagt
     3661 ggatcattgc atccattgtt gcagcagatt gtcctttatc tatccaatct tgacgaaaac
     3721 gatctgtaaa gaaccatata ggaatccact gtccggcagc actgcctaat tgcattagag
     3781 cccaagacgc aacttgagga attgctcgat catatccaaa tacagataga ataatatcag
     3841 ctaagatcgc catactaatc gaactgataa accaaggtaa gtacgccccg cccattaacg
     3901 taaaaaataa caataaaaca attgtgtaaa tagtaaaaat tccttttcta gggtagttat
     3961 agctaatata aacaaatatg attccagata ataatccaaa aatcccgggg gatatgctgt
     4021 gaccaaacgc cccaccaata ctactaacca ttattgaaaa aaagtaaatt actag
//
LOCUS       DAZKNH010000144         4056 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000144 DAZKNH010000000
VERSION     DAZKNH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.148"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..1355
                     /locus_tag="ACG8MG_002551"
     CDS             54..1355
                     /locus_tag="ACG8MG_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0376542.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1382..1642
                     /locus_tag="ACG8MG_002552"
     CDS             1382..1642
                     /locus_tag="ACG8MG_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0376543.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2641..3876)
                     /locus_tag="ACG8MG_002553"
     CDS             complement(2641..3876)
                     /locus_tag="ACG8MG_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0376544.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1153 a          660 c          851 g         1392 t
ORIGIN      
        1 ttgcgttgtc atgcaatgaa tggttcatat tttattgaaa aggagtggtt ttagtggaaa
       61 agctgatgaa atgggtcgaa gagaaacttg tcccaccaat ggcaaaaatt ggtacacagc
      121 gtcatttatt agcaattcgt aatggggttg tttcaacatt gtcattgatt ttgattggga
      181 cattttttat ggtctttatc aatttaccgt ttcctggatg gaatgaattt attgcccctt
      241 attcggcaac gattgtttta ccctttagga tgacgatggg attgatggct atttatgcaa
      301 cttttgttat gggttcggat ctagctaaat catatggatt agatagtgtg acaggtggta
      361 tattatcttt aggaacgttc tttatgttac aagttcctgt aaacgtttta actcctgaag
      421 aggcacctct tggctgggtc ttacctatga gttctttggg agcatctggg atgtttgcgg
      481 ggatcttatc tatgattttt gcagtggaag tatatcgttt ctttaaaaat cgaaatatta
      541 ctatcaaaat gccagaacaa gtgccacctg ctgttgcccg tagttttgag gcattgattc
      601 caggtgcagt cattttaaca attacttggc tgattcgaag cgtcgcgggc tttgatgtaa
      661 acgctgcctt attatcgtta ttcgagcctt taaccaatat tttagggaat aatttgcttg
      721 gtgttttact accaatgttt ttgatccatt tgttgtggtc atttggtatc catggcatga
      781 gtattgtagg ggctgttgtt cgtccaatgt ggctgatcat gttggatgag aatgcaaagg
      841 cattggcaga tggtacagct gccacccaat taccctatat cgctcctgaa cagttttatc
      901 aatggacggt aacaattggt ggtgcaggag caacgatcgt ggtatctgtt ttgtttatat
      961 tcttctgtaa atcaaaattc ttgaaagagg ttggtcgttt ttctattatt cctggtatct
     1021 tcaatatcaa tgagcccata atttttggag ctcctatgat gttgaatcct tacatgttca
     1081 tcccgttcaa cttagttcct ttagttttga ctattatcag ttattttgca gtcaaattag
     1141 aaatggtcaa tagatttact gttttgccaa catggacatt accagctcca atcggcggtt
     1201 atctttcagc aggaaatgac tggcgtgttg ttatcttgat catgatcaac acattgatag
     1261 catttattat ctattatcca tttgtaaaag cttatgataa aaagatgctg tcagatgaac
     1321 aaagtcgtgg agaaaacgaa gtccagactg tctaagtttg gtttttagaa ggtgggaaac
     1381 aatgtattgg ctgattttat tttttgtttt tatcttttta ttaacggcta gtcaccttat
     1441 attgaatatg ctagctacat atcatattca aatcaatcga tggatttggg cactggcatc
     1501 atttttgatc gtgattcttc caaagatcat tgtaccgcat atgaatgttt tgttttcatg
     1561 ggggacatat gtactttgtg gaatttttgc tatcaatttt atgatcgagc agcatagatg
     1621 gtttgtcaca tcgaagcttt aaatgttcta aaagtggttg taacagaagt ggttagcttc
     1681 aagaaataag aagaaattta cgaaaattgc ttcttaaatt tttgtgaaat gttggcttat
     1741 ttttcgtttg gttgaactta ttaccgcaga tgcaagcttt tgaccaccgt ttattcggaa
     1801 tatgggactg tgacaaggct tttgtcacag ccctttttta cataaagatt attattgttt
     1861 taactgtttt ggcagaaaga aggaggaaaa aatggaaatt ttatcagcaa tggatttaga
     1921 attagtagat ctactaccaa agtgatcaag tcaaatatag atttgggagc ttacaaccat
     1981 atagtagggg ctgcagtatg gtgtatgaat ggtaatatgt atgatgtgag tgtttattca
     2041 aatcatgaaa cgtgtataga aataattgtg ctgggaatag caatcagtca tggtgaacgt
     2101 gacttttagt gtatcgttgc tgacgacata gaacattctg acatgatcta ctcaatatgt
     2161 ggggattgct ggcagtttat ataggagtat gcatcggatt gcgagatcat tgtctcaaca
     2221 gaagagggag gttgattatc aaagataaag tggtaaatcc tacatttgat ttagtaattg
     2281 ttgaggggac tagcggcaca aatctctaca aagcgatgct tctagatcta tatgacacat
     2341 tttaagaaaa tgtatttgcc taattattat ggtctttctt ttgaagaaac gttgagatga
     2401 tccaaccaga gaaaggtgtc aaagaatttg actaagaata aatggcagac gctacctatg
     2461 ctcatttgga tagcgtcaga tcttttattt taaggatgat tttgtgtttt ttagacaccg
     2521 taatttaagt aactctaact tttcaatcca ctatttactt tttttgtatt ttcactgtct
     2581 gggtctcgaa tagcgaaatt actgatgagt ctctattaaa aaagcttttt ctacttgaaa
     2641 ctatattggg tggttgctca ggaaggtttc gatttcttga attgcttgtt gtggaccatt
     2701 tattggaaaa acggcttgca gcgtgcgtgc ctgctgacga atcttgggtg aagaaacttc
     2761 gcgcagagca atctgcagtt ttctttcagt cactggtttt gtgagggcga ttgcgtttcc
     2821 gtattttaca caatagtcaa tattacagct ttgctcaaat tgcatcccca caccgataaa
     2881 cggtttgccg gattcacagg cagtttggac ggtcccttcc ccgccgtgga taaaagaaaa
     2941 atcaatgaat tcatgcaaaa gatgactaga cagatattct gttattaaaa gatcttctga
     3001 gtcagattga atgtcatcaa gcagatagtc aataggtgca ataaccgcta gatccggttg
     3061 tttttgtaag aagaacaaaa ttttgcgtaa catacgcgga ttgcctgaac tacccattgc
     3121 gaaatagata atacgttttc tttcttgttg ctttcgctgt ataaaagtta gcacttcttc
     3181 aggcaaggaa gtactgagct tagcaaaaat tggaccaacg attcgataat tttccggcag
     3241 ctgttcttct tccaaaaata gtttaggcgt tgtgatgaga tttagatcag catccatcat
     3301 ttgtaaactt gtttttggta aaggaagcat ttctttttgt gcagcgtgtt tgaaggcatt
     3361 ggggatccat cgggtattta ctatacgctt ttttgtccag tgccaaaggg gattgcttaa
     3421 agggccgaag aaacgtaact cgtggggcat cagcgcatgc tctgattgaa aaaaaggttc
     3481 actcaaagca tagggtttaa catatactaa aggaatcttt ttattgcggg ccgataaaaa
     3541 aattgtcaca ttcgaacctg tcacaatcag tcgaggctca aacttagcaa tcagactctg
     3601 ttcactagtg atacgagagg caaccatttc tttggtaaaa gggtttttaa gtgtacgcaa
     3661 ctggtcaaat tttatgattt gttcttcttg ttttttcgta aggatcggcg ctagtaaatg
     3721 atactcaaag ccactttctg tgatcaaagg agcaaaagtt ttagaaaagc caaagaatac
     3781 acagtaataa cggtctttca tagctttggc aacctcaatc attcgtgttg tctcagctaa
     3841 attgaatact gcaggagcaa aaagaattgt cttcatgaaa tcctccttat tggaatgagt
     3901 ctatcccttc gtttcttact agtatactaa aaaacacagc gttcgagtag cctatgtata
     3961 ttttgaatat taaactgtct ctcgctgttt tcacaacttc taaatttaaa ttttgtaatc
     4021 aagttgaaac tccaattttc aaaaaaacac gcaaca
//
LOCUS       DAZKNH010000145         4043 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000145 DAZKNH010000000
VERSION     DAZKNH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.149"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(153..1658)
                     /locus_tag="ACG8MG_002554"
     CDS             complement(153..1658)
                     /locus_tag="ACG8MG_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0376545.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1655..2341)
                     /locus_tag="ACG8MG_002555"
     CDS             complement(1655..2341)
                     /locus_tag="ACG8MG_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0376546.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2534..3955)
                     /gene="gndA"
                     /locus_tag="ACG8MG_002556"
     CDS             complement(2534..3955)
                     /gene="gndA"
                     /locus_tag="ACG8MG_002556"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFQ0376547.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1116 a          856 c          691 g         1380 t
ORIGIN      
        1 catctaaaag atacttacat ttaattaacg gatgttattc tatttaattg ttcgctagtt
       61 tttttataat gacatccctg aatttctcac ttgcttgtat tttgtacctt attctttgtt
      121 ggatatataa aaaaacaagt aaagcacatg ctttacttgt cttctgcctt tgcgatagga
      181 ataaagattc taaaaattgt cccttgccct actacacttt ctgctaagat acgtccttta
      241 tagttttcaa ccagttgttt agcgatagac aaccccagac catttccacc cttcgttcta
      301 gctcttgcat tatccactcg ataaaaacga ttgaagatct tattaaggtc ttctttgggt
      361 attccttcgc caaagtcctg aatcgcgatc tcaaattcat tcagagtgga agaaatagag
      421 atatggactt cttttcgtgt agttgaatac tttactgcgt tatccaatat gataatgatt
      481 aattgttcaa agtgattacg ataaatttgc agggtcactt cttttgtcaa atcatcatct
      541 aaagtgatca catagtctgg atataatatc ttgaaattat tataaacttg ataggtgact
      601 tcttttcctt tagttgtttc attcgcataa tgaacatcta cttgttctgc tcttgataag
      661 tctagcatct cttgaactaa gcttttcata cggctgattt cttgcatgct ggcgcttaat
      721 gattcttcta atatctcagg atcatctttc ccccaacgat ttagcagact aagatgccct
      781 tcaataattg caacaggtgt ccttaactca tgagatacgt cttccacgaa ttgctcttgt
      841 tgttcaatat acaaacgcat acggtcaagc atctcattga atatttcagc taaatctgcc
      901 aattcatcat ttgtgtcgat ttcaggcatg tggatatctg attgcggatc tttacgtata
      961 gtatccattg tatctctgag taccttgagt ggttttaaga aataagaaga taaaaagaaa
     1021 cctaaaatac tacttaagat caaagaaaca acttctaaaa caataagagt caataatagc
     1081 ttatttcgaa tatcatagaa agaagataat tcatagaagc tttgcgcata gccgatcttt
     1141 tctcttgtac ctttagaata tataggctgg atagataaaa agccagtttt tccttctagt
     1201 gtaacgatcg ttggaacttt acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt
     1261 ttaaaaatca agttctcgtc caaatcataa acataaagat ccattgatgg ctgccctagc
     1321 tcagctataa aggaatcgat cttcattaag tttccttcga ccagtgaagt atgatctacg
     1381 acctcatctc cgtcatttgt cgctttcgtc agattacgat acgtatttac taatgtcagt
     1441 tcttcatccg agttagccaa acgagaagct acttctgaaa tcgtacgctc tacattgttt
     1501 ctttcttttg ccacaatcag attgatagat gatttatagg taatgacagc gaaaattgta
     1561 aacacaacga atatgaagaa agaactcgca aaggcccact tgattgtcaa ggagggccct
     1621 ttcagttctc ttttttccga ttttttcggc tttatcacga gcgcatcaca taacctgttc
     1681 cgcgtacagt ttggatatag ctttcctccc cgggaacgtc aattttgttg cgaagataac
     1741 gaatatatac gtccactacg tttgtttcaa cttctgtttc gtatccccac actttattta
     1801 gtaaaacgtc acgtgctaat accacgttca cgttttccat taaagtaagc agcaactcgt
     1861 attctctttt tgttagttca attacttcat ttccgcgacg tacgacacgg ttttcttttt
     1921 cgatagtcaa atcgcgataa gtaatagtag tttgtttagc cacattttta tcgccttcaa
     1981 tatcgattcg acgcaacaat gcacgaagac gtgctaataa ttcttcgata gcaaatggtt
     2041 tgacgatgta gtcatctgct ccgtggtcaa gtccagatac acgatcgatc acagaatctc
     2101 ttgcagtcat cataatgatt ggtgtatttt tgacttggcg tacacgtctg cagacttcta
     2161 aaccgttcaa ttctgggagc atcagatcca acaatatggc atcccactca ttatttaatg
     2221 ccgcatctaa accagttcgg ccgttgtagt ggacttctgc gttataccct tcatgcttca
     2281 actctagctc gacaaatcgt gccaagttct tttcatcttc aatgattaga atattgctca
     2341 tttattattt tatcctttct attctactgg ctttttaaaa actctatcat tataccgata
     2401 atatgcttaa aaatctatag tcattttatc atggatagca attttaaaaa agagctgaag
     2461 tctactcata acaatcgact tcagctcttt cgtcctcttt ttattttctc atttcctaac
     2521 agccgaaaga ctattattct tcatggtacc atgagtaatg gaagattcct tctttgtcga
     2581 cacgctcata ggtatgtgcg ccaaagtaat cgcgttgggc ttggattata tttgcaggca
     2641 gacgttctgc tctgtatgaa tcgaagtaag cgatggctga tgagaaagtc ggtacaggta
     2701 cgccagcttg gacagccaag gcaacgacgt ctctgactga ttgctgatat tttttcgtga
     2761 tttcaacgaa gtaatcatct agcaataaat tttcgatctc aggatttttg tcataggcat
     2821 ccgtaatttt ttgcaagaat cgagctcgga tgatacatcc agcgcgccag atttttgcga
     2881 tttcaccaaa tggcaagtcc caatcaaatt cttttgaagc gacacgtaat tgagcaaatc
     2941 cttgggcata actcatgatt ttactgaaat acaacgcttc tcggattttt tcgaccaatt
     3001 cttttttgtc tcccgtgaac tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta
     3061 cgcgttcttc tttataagca gaaatatagc gggcaaatac agattcagtg atcaatggaa
     3121 gtggcacacc taagtcaagc gcgctttggc ttgtccattt acctgttcct ttattcccag
     3181 ctgcatcaag gatcacatct actactggtt tacctgttcc ttcatcgtct ttacgtgtca
     3241 gaatgtcagc agtgatttct atcaaatagc tgtctaattc gccttgattc cattctttaa
     3301 agatatcagc catttcttct actgaaaggt ttaaaatatt cttcattaag tcataagatt
     3361 cagcaatcag ttgcatatct ccatactcaa taccgttgtg taccattttc acataatgtc
     3421 cggctccatt tgggccaata tatgtgacac atggtgctcc gtcttctgct tttgcagaaa
     3481 tttgctctaa gattggtgca actagcgcat aggcttcttt ttgtccacca ggcatgattg
     3541 aaggtccttt taaagctcct tcttcaccac cggaaacgcc tgtaccgata aagttgatgc
     3601 cagagttggc taactcttca ttacgacgca tcgtatcttt aaagaatgta tttcctccgt
     3661 caatcaaaat atcgccttta tcaaggtgag gaagtaactc ttggattgtt gcatctgttg
     3721 ccggtcctgc tttgaccatt agcaagatcc gacgtggttt ctcgatagat tctacaaatt
     3781 cttcgattgt atatgttgct ttgaaatttt tatctgggtg ttcttctact acagcggttg
     3841 ttttagcccc tgtacgattg aataaagcca ctgaatagcc gcggctttct atgttcaagg
     3901 ctagattttt acccataacg gccataccga caacgccgat ttgttgtttt gacatatgaa
     3961 ctcctcctac atacgggatt tctccctaat ctcttacatt atagcttaac acggctacat
     4021 tgcaaactaa agaacaacat att
//
LOCUS       DAZKNH010000146         4034 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000146 DAZKNH010000000
VERSION     DAZKNH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.150"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACG8MG_002557"
     CDS             73..234
                     /locus_tag="ACG8MG_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFQ0376548.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="ACG8MG_002558"
     CDS             253..1530
                     /locus_tag="ACG8MG_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0376549.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="ACG8MG_002559"
     CDS             1540..2196
                     /locus_tag="ACG8MG_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0376550.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="ACG8MG_002560"
     CDS             2196..3572
                     /locus_tag="ACG8MG_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0376551.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..>4034
                     /locus_tag="ACG8MG_002561"
     CDS             3702..>4034
                     /locus_tag="ACG8MG_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0376552.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1612 a          440 c          704 g         1278 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catc
//
LOCUS       DAZKNH010000147         4015 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000147 DAZKNH010000000
VERSION     DAZKNH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.151"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..722)
                     /locus_tag="ACG8MG_002562"
     CDS             complement(<1..722)
                     /locus_tag="ACG8MG_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0376553.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPII"
     gene            complement(735..2711)
                     /locus_tag="ACG8MG_002563"
     CDS             complement(735..2711)
                     /locus_tag="ACG8MG_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0376554.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            complement(2764..3435)
                     /locus_tag="ACG8MG_002564"
     CDS             complement(2764..3435)
                     /locus_tag="ACG8MG_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0376555.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(3495..3704)
                     /locus_tag="ACG8MG_002565"
     CDS             complement(3495..3704)
                     /locus_tag="ACG8MG_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376556.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(3771..4010)
                     /locus_tag="ACG8MG_002566"
     CDS             complement(3771..4010)
                     /locus_tag="ACG8MG_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376557.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
BASE COUNT         1078 a          861 c          600 g         1476 t
ORIGIN      
        1 aaaataatag gaacgcaaaa aaaagcgaat agcagtgcta atcgccaatc caatgaaatt
       61 gatttcctat tttttgcttt tttctgtagt tttaaatctt ctttactgaa ggaaactcgt
      121 tcaccataca ctaaaaagcg gtgtgagttg acaccttttg gtgtgcatgt cattaacgtg
      181 atcctatctt ggtctttttc aggcataaga taatctaaat aatttttttg taattcttct
      241 gctttaactt cttcaattcg ataaatttga taggcatgtt gttcttcatt taccttaata
      301 aagaattttt gccctttttt tactgaaggc aaatcatcga aaagattatc tttataaagt
      361 ccactgtgcc ccgcgattaa aggattttta ccgtttcctc ctgtaatatc gccagttcct
      421 tccgtaatcc ctacaccatt ctctaaaatt ttgtcggaag ttccgtcata aattggtagt
      481 tttaaagaaa tacttggaat tgtaagaaca ccaacaggat cgcctaactc tattttcgca
      541 acatccaaag aagaatctgt tttttcaaga ttttcaatgg ctaaaattct ttgctgttgt
      601 tgaaatttcg ctacatttgt ttccacaact ggaattaaat agactgctac tgccgacagc
      661 aaccaaaaaa atatcatcag tttgtgcatg actcttcttt ttgggggctt acgaataatc
      721 attagctgat ccttctaggc tttcttctta aagtaaacaa ctgcagaaag tccccctgct
      781 cctagcaaca agcaagagat caatacgtaa gattggaaac cgttgctacc agtcattggt
      841 acggaagctt tttgattatt tttgatcgtg attcggtttt cttctttgta tgaatctttg
      901 tctactttga aagagatctc ttttgttaat ttttgatagt tttcaggagc ttttgtttct
      961 tccagtgagt aatctccttc agttaaacca gtgatttcaa aatgaccctc tttatctgat
     1021 tttaggattg ctgcatttgc tacatcgtct acccaaactg cttctttgac accatcttta
     1081 tctgtatcag taagtccacc gtagtactta ccttctgcat tcttagctgc aaattctgcg
     1141 ccttcaagcg gtgttgcttt gatacctgtt ccaaataggc cgctatcgac ttttaagaat
     1201 tttgcgccat aagtcacaac gtcagctttc gttttttcgt ggtgttcttc ctgattaaat
     1261 ggattattgt tataaacgaa atccacgcta tttgttaatg gatttgcgat ttcagcacta
     1321 tcattcacaa acatgttata agtcattttt agatcttgac catgtaaatt cgcaactgta
     1381 tcacttactt gtccattatt tacgataaaa tcgatcacaa aaccactatc agaattttcg
     1441 attttatagt cagtattttc ttttaatacg actgaaccat cattatttgt tactttaata
     1501 tctgcttttt cagcaacata agaaagtcct ttacctgctg tatctcccca tttaaataca
     1561 ctgtaaggag aagtttcgct tttcacaccg tttactgtga taccgtctgc gccacctgca
     1621 atgtctgttg gaatatgaat cgttccttga taatttactg cgtcaccaat ttggaaagaa
     1681 tgttcttgtc ctgttccatt tgttactgtt ttatcgatca ctggtgcttt atagtttacg
     1741 tatgaagctt ttaagtcact tgtcacgcca tcattcgtaa tttcaaaggt taatttattg
     1801 tgcgcatcgt tgcgagcgtc tgcgcctaat aaatattgat ctgctgttgg ttcttgatct
     1861 gatcccacta caacttccga aggaacttcc acaaaatagt attttcctaa cgtcaaatca
     1921 gtcgcagtca gctttccttg atcatccgat acaagatcac ccaattttgt tccttttcca
     1981 ggttcgccct tgtataactc aaattttgcc ccttttaaaa gggtttgttt gctttggtct
     2041 gcgccatcgt caccaaattt cgttaattca aaactcaact tagaaacaat gttcttagga
     2101 tacaaatgaa tattttttaa aaatcctgat cctgttttat ctgtcattgg cgcaatcaca
     2161 accattggtt ttgctttttg tgtgactaat ccagcagcgg atttactttc ataaaccaca
     2221 tatacattcc ctttggaatt gacataggct ggttgatcag taaaagtgat ctcaccattt
     2281 tcatctacag ctgaagtaat tttgttggtt gcattcttga cgtactcact gttagatccc
     2341 ttgtcttcta catcttgtac aatctcgtcc actttcgcgt tggttagatc agaatcttct
     2401 ctcggtaata cttgatccgt aatatttcct aacgtaaact caacatcacc ataatctgcc
     2461 ttgttgtatt tcgttacacc tgtaggatat tgatcgtgcg tataaccatc gtttttgatc
     2521 ccgtcaatat caacattcaa ttgcgttcct tgatcgtaca ttaatttatg aaccgtgata
     2581 ttgaccttct ctggtggtgt tttttgcgtg acatcttctg catgtacttg atacccctga
     2641 aagcagcctg caatgattcc taacattcca acaagcattc cagctttttt tacataccga
     2701 tttattgcca ttttttactc tcctcatttc atatttcaac gactcgcgtt gattaaaacc
     2761 ttgttattgt agctctccct gtaccacgta tctttctttt ccttgtgacg tataaacaca
     2821 agtaaacaac gttaataagg gtttctctcc tttctttggc aaagacaaga aagagttctc
     2881 ttcaggaacg ccatcgatta atttaaagta cccaggtacg ataaatttac gcgttacctt
     2941 gtaacgatag acgccatcgt tactaactaa gtccatcacc tcccctaatg acaacttttg
     3001 aatatcagaa aataaaacac ctggcatttc catgttatga cctgctagga catagtttcc
     3061 tttgcccatt tcggcatctt cataaaaata agtagccgct ccaagactca aagtgtattg
     3121 atttgcccct ttaaaaattg gcaatgctaa accaagtgca ggaatctgaa tttgtgcaaa
     3181 tacttgttta ttgacgccca tttcgattgc ttctttttgt atggacaaca tttgccccaa
     3241 agacatatca ttattgacgt tcgttgcatc ctccttataa ctctttgcat gtgcattgat
     3301 ttgatctacg ggcatctttt tttctatatt ttgcttaaac acctctcctt catgtttttg
     3361 gtagctaaca tagccaaaca cactgcaaaa gacgattaaa gtgactccta aaaaaccaaa
     3421 taaccatttt ttcatccatc tgcctccttt cagacaaaac aaaaaaactt tttctcattt
     3481 ccatagaaaa agttttaaaa atgaattcta taagttcgtc tcaactgttc aaagtttttc
     3541 attgaaccta acaaacaagc tttctctttt cctgttaact gcatatcatt gatatattgc
     3601 gcgacagata atcttttaag gtgtttttgc ttcatcgttt ttagtaactt ttcttccatt
     3661 tctttcgaca ctttttttct gttttcgatt tcttcataat tcatgagttt tcctaccttc
     3721 cttgtttttt gcaaaaataa aaaagcctat gatcacatac gctctacact tcagtcttgg
     3781 gttatctttt tctttcgttt aattactggc cgtgctggtt tcaaatcctt gattccatta
     3841 tgggttctcc aataatcgaa ttcttttcgc aatctttctt tttccatatc tgttccttta
     3901 gataaggctt tgaatacttc tttaggtgaa taaccacgag ctaacagctg tacatacctt
     3961 ccttttgtca aagtatccat atccatcgcc tctaaaactg gtcgcgccat ctact
//
LOCUS       DAZKNH010000148         3955 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000148 DAZKNH010000000
VERSION     DAZKNH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.152"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(120..662)
                     /locus_tag="ACG8MG_002567"
     CDS             complement(120..662)
                     /locus_tag="ACG8MG_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFQ0376558.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            797..1186
                     /locus_tag="ACG8MG_002568"
     CDS             797..1186
                     /locus_tag="ACG8MG_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376559.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1190..1954
                     /locus_tag="ACG8MG_002569"
     CDS             1190..1954
                     /locus_tag="ACG8MG_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0376560.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2083..3030
                     /locus_tag="ACG8MG_002570"
     CDS             2083..3030
                     /locus_tag="ACG8MG_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0376561.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3160..3900
                     /locus_tag="ACG8MG_002571"
     CDS             3160..3900
                     /locus_tag="ACG8MG_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376562.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1318 a          669 c          806 g         1162 t
ORIGIN      
        1 ataaagtata ataacaaatt gatgagtata tgtcgatgtt ttttaatcaa aataataaat
       61 aaaaactgtc taacatttaa aatttaattt agatattaga cagtttttaa gtttgtgagt
      121 tattttcctt gtcgttttct cactttttta gtccagaaac cacctgaaat atattttggc
      181 tcataagaaa tgataaaagc acgctcttcg atttctttga tcaggcgata cagttcacgt
      241 tcgctttttc taggagaaag tatttcgaga actagccgct caccttccag ccccattgca
      301 gaagtctgtg tcacaccata accgtgttct ctcagtatac ccggcaaact tttatcttct
      361 gcgctatttg ctggcaaaat cgttgtgacc atgatgtatc ctaatgccaa tttgtcttca
      421 atcaaaattc cgacactgat acctactgcg taacctaacg catagacaaa taaattcagt
      481 ggattatcta aacgatctaa aacaagggat aagccgacga tataaatcgt gatctcgacc
      541 atactgacaa gtggagcaat cagtcgatag cctttcattg tcaacatgaa acgtaacgtg
      601 ttcagagtga tataagcaaa attaatgagg aagatcatcc ccaacatttt tggatcaatc
      661 atttgtaaaa ctcctttcat ttatataaca acaatatcaa aaaaaatcgt aagtgaagaa
      721 aaattgaaac tttatacata caaaaaaatg ataaaatgat tataatagaa ttataaagat
      781 aggttaggtg attaaaatga aaacggtcaa tcgtgtgttt tgtgccagcc acgtgctaag
      841 tctccttctc agtatatact taggtgtcta ttcgattcaa cagccaattg aggtagagaa
      901 cattgttttt cagatgtcgc tgacagatgg tttaatttta agcgtttttt tctttttgat
      961 actgtttatt ggtaatattt tcggtagcgt tgccagtttt ttgaattttt ctatttatcc
     1021 actcctttcg ctagcattag gagtggtggg actttgttct ctgtgcttat ttcccgagcc
     1081 attcagtcct gcatttatat tatttggtac actgaacctg ttccagacaa ctgttggagc
     1141 atggttgcta tggcgatcag gaaatttaat gaaaataggt gagtaacaat tgaatccaat
     1201 tatattgaaa cgttctgtcc atcatattcc agtgttagaa gttgttctga aagaattaag
     1261 agaggaacgt attcccgttg tcatctatta tcacggatgg cagacaagta aggagttagt
     1321 tttgacgcaa ggaagaaaaa ttgcgcagca gggcattcgt gtgatcctgc cagatgcgat
     1381 gaatcatggg gagcgcaaac agccggtttc ttctattcca tcctatacat tttgggatag
     1441 tatctacgga aatctgtttg aatttgacac acttatcaag catttagaaa aaagaggtct
     1501 tctacatgat cagcttgcag ttggcggggt ttcgatgggg ggaatgacaa catgcgcctt
     1561 gttggctaaa catcctcata tagtagcggg cgtgtgtttg atgggttccc ctgcaccttt
     1621 ggaatatgga aaagaaatcg taaggcgcgc atcggaatat aattatgtcg taccaaaaga
     1681 ctattttgat ttgatttcat gggttaagag ctatgatttg tcattgcagc cggaaaagtt
     1741 agctggacgt cctctgttct tttggcatgg acaagctgac gaaaaaatcc cttatataca
     1801 aacggaacga tttgtagaaa aaaatcggca taagtcttac ggaaaagaaa ttgtttttcg
     1861 ttcctcaaaa acacagcgac atatggtaca aataccacta atggaagaag cttccacttt
     1921 tttagcaaaa aatttgataa aaaagtataa ataattgaaa cggaaaatca ctcatgacga
     1981 tagaggattt tccgtttttt attttgggct ataataaact ctgttaggaa aatttggtat
     2041 aattgatagt aagaatgaca acatgttaga aaggaagaga gcatgcccac tttagcagat
     2101 gttgcaaaac gtgccaatgt ttcaaaaatg acggtctctc gagtcatcaa tcatccggaa
     2161 caggtgactg acgaattgaa agagttagtt ttctcagcaa tggctgaact ggattaccgt
     2221 ccaaatattg ctgcaaaagc acttgtcacg aatcggtcgc agatcatcaa attatttata
     2281 ctggaagaaa tcgacacaac tgagccctat tacatgaatt tgctgatggg aatcgctaaa
     2341 cggatcggta aagcgcatta ttctctccaa ttagttacaa atgatgcctt cgacgtcggt
     2401 tcttgtgatg gatacatcat cacaggtgtc agaaaaaaag acttcgattg gatcgaccgt
     2461 ttggaaaaac cagttgtctt gtttggggaa aattctgaag gatatgatta tgtagacagc
     2521 gataatgaat atggtacagc aaaagcaacg caatatgcct tagaaagaga ttatcaaacg
     2581 attatttata tcggtatcga agtggacgag ccttttgaaa tttcaaggga aactggttat
     2641 ttatctgtga tgaaggaagt tgatcttcct agtaaaatct tgcggtttcc aaatcattcg
     2701 acagaagtag aaagatatat gacagaaaac tgggcagatt ttacagataa aacttgtttt
     2761 gtatgtagtt cagatcggct agcactagga gcagtaagag ggattataaa tgctggagga
     2821 cacttgcccg aagaatttgg tgtgattgga tttgatgggg tatttttaga ccaaatcagt
     2881 tcacctaaat tgacaacgat caagcaagat attatacgta tgggtgaagt atgtggagaa
     2941 atgttgttaa aaaaaataga agaaaaaggg gcgagtcaag gttatcgtcg tttttttcct
     3001 gaactagtta tcagagagac aacgaattaa gtcttattac tatatatata tttatgaaat
     3061 tcatcgctat tttcttaaaa taatcagcgc attgctactg aaaacaatga atctgtgcta
     3121 ttattgagaa aagttccatt tttgagaagg atggtttgaa tgaaagaaat cagacgaaaa
     3181 catttccgtt ttcctgcgta cgatgatgag ttaggcgtaa aattaaccaa cgacaagaag
     3241 cgagtattat ttgacggaca agaagatctt ttgactgaac agaatcagtc cccgtttgaa
     3301 cagatgaaag aatttcattt cacgaacacg aacaccgaga aaatccgtcg caagcgtcgt
     3361 gcaacaaatg gattgaagtc agctccgcgt aaggaaaatt tggaaaagca taaaggaaaa
     3421 ttaccggatt ataccggcca ttaccaatca gaaatcacat cgacaggacg aaaaaaactt
     3481 tttggtgatt cctctcgttc tgtcagacaa agtgaaacaa ggacagagtc caagcatact
     3541 acacgaaacg catctgttga cacgaaggtg cgggagcgtt cttattttgt tcctaaatat
     3601 gtacccgcat cgattatccc agaccctaga gaaagcaaaa tttctgagga tcatttaatc
     3661 gaatcgatga aaaaaacgga tgactcttat ctgttgttcg atacagaacc gacgccgtat
     3721 caagaaaaaa gaaatggtga gccgtctgta cgaacattca atcgaacaaa aacgttagag
     3781 gaaacaaagc agtcagaaga aaaaaaatcg aaaaaaagcc atggtatctt agaacgctca
     3841 ttacaaggat tgatcgatga tcaaggaaac tctttgggag aaaatagcta ttttaaataa
     3901 gtgatataca aatttatatt cggctacagt tggaaaggaa acgctgcggc gtttc
//
LOCUS       DAZKNH010000149         3925 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000149 DAZKNH010000000
VERSION     DAZKNH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.153"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(573..1130)
                     /locus_tag="ACG8MG_002572"
     CDS             complement(573..1130)
                     /locus_tag="ACG8MG_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376563.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="ACG8MG_002573"
     CDS             complement(1309..1920)
                     /locus_tag="ACG8MG_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376564.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="ACG8MG_002574"
     CDS             complement(2033..2290)
                     /locus_tag="ACG8MG_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376565.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="ACG8MG_002575"
     CDS             complement(2887..3165)
                     /locus_tag="ACG8MG_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376566.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="ACG8MG_002576"
     CDS             complement(3199..3450)
                     /locus_tag="ACG8MG_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376567.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DAZKNH010000150         3912 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000150 DAZKNH010000000
VERSION     DAZKNH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.154"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(425..1492)
                     /locus_tag="ACG8MG_002577"
     CDS             complement(425..1492)
                     /locus_tag="ACG8MG_002577"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0376568.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(1512..1991)
                     /gene="pssE"
                     /locus_tag="ACG8MG_002578"
     CDS             complement(1512..1991)
                     /gene="pssE"
                     /locus_tag="ACG8MG_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssE/Cps14G family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0376569.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(2008..2469)
                     /gene="pssD"
                     /locus_tag="ACG8MG_002579"
     CDS             complement(2008..2469)
                     /gene="pssD"
                     /locus_tag="ACG8MG_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0376570.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(2483..3733)
                     /locus_tag="ACG8MG_002580"
     CDS             complement(2483..3733)
                     /locus_tag="ACG8MG_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFQ0376571.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
BASE COUNT         1215 a          814 c          509 g         1374 t
ORIGIN      
        1 tatttattaa atcaataata tgatcctcgt aagtatgctt tcccttggaa taatgaaaat
       61 atacttttgt attacacaat attgcaattt tatttcttaa tatgattatt tcactcattc
      121 attactgcca catatacaca acttataacc accccctgca aagtttaggg taagttatca
      181 gataatatct tcctcttcat catccagaat acaaaatcta tttatttata gaaatatcat
      241 taatgttttt atataatgta ccgtgcttgt actcttgctc tattacaagc actaaaacac
      301 tactattcat ttgaattctt acacggataa aaatttacat cgtctatttc aatatttttt
      361 ctctctattt tattcactta ataagttcat tacataatcg aaacattttt tattttcctt
      421 gtcatcactc cttgctccct ttttgataca gatttaaaat tctatcaaag aaatttccat
      481 cgttaaattt cgttattgcc aaatccttcg aacaacgtga aaattttacg tatttatctt
      541 tatcattgat ttttagtatt gcagatgtca acgctgatac gtcaccaggt tgaaacaaat
      601 atccattttc tccatcacgt actgctgaag aaatatctcc tacatctgta gcaattacag
      661 gcattccata agaaatcgcc tctagaatag atataggcaa tccctcgtta taggacggta
      721 aaatcatcac atttgatctt ctatagcact cctccttttt ttctcccaca acccaacctg
      781 taaattcaaa atacgattgc aaattatatt cttctatcaa ttctttgagc gatatttctt
      841 catcacctgc tccagcaatc tcaaaaataa actttttttc tggatattgt tctgcaatgt
      901 ttttaactgc ttctaccaaa tcgtgtacac cttttcgctt tatcaaaact cccataaaca
      961 gaaatcggca catttttgaa ttccacttta tggtatattc tggaactaca atagaatttt
     1021 ctacaacaac agttttacaa tttggctcaa tttctttaat tatcttcttc catttttctc
     1081 caagtacaat cgtggaatca gactctctta acagcaagcg aatctgattt tgttttttta
     1141 cagaaacact atcataccat tttttaaatt ccgatccatg aagatggatg attacaggaa
     1201 tatttaataa tctaaaccac ttattcagca catatttcct actaaaactc cctctgtacg
     1261 acatgtgcat atgaataata ttaggtttac tcaataaaga gatacctata attttagcaa
     1321 atgcatacat aaaatataaa gatttaaata ttgcatttcc acccttataa ctcggtataa
     1381 aagtcatctc gatattcttt ttttcccaat taaaattgag catttgtgta attatacttg
     1441 taattccacc ttttaccgag ctgtcattac caatcataag tactctttgc ataacgcttt
     1501 actccttata tttatatttt ttctattaat acctctatcg aattaataat cgtttccgtg
     1561 ttagatacat acttgttaaa catccgaact cttgtttctt taacggcatc ttctaaattt
     1621 tctaattcat aacaaggata gataatattc atctcatcaa attgtctaat taactgcagt
     1681 tggtgatcat cgacatgctc accatactta gccaatcgag gaacagcaat gaccctttta
     1741 ccttttttta cggccccaat tatagcacct gtacctccat gagtaatcac catttctgct
     1801 ttttgcatag tttcagtaaa ctcatcccta tttagaaatg ctttatagga ataatttagt
     1861 ggtttataat cactataacc actctgtgca aatgtacttt cgattattac tcctttttca
     1921 attaaatcat caatagcttt taacaatcta tcaaattgaa atttttgtga acctaatgtt
     1981 ataaatatca tttttttctc ctattattta atatatccca cccaaataaa tagcattagg
     2041 ataaaattgt agcatagttt cccactgaac ataaaattga tctgcaaaat gatacatcaa
     2101 ttttcctgtc tgagtagggc tggttacttt tgcaaacgat tcaatataaa tcagtttttt
     2161 tcccatcatt tttgttatta agcagatagg gatcatagct aaaacacctg tacaaatcac
     2221 gagatccggt ttttccttta tatatataat aattgatttg aataaattaa taagtatagt
     2281 acacaaaaaa aacttttctt ttctatttac ttgtctcatg tagtacgttt tttcgccttt
     2341 tgactcagct gaatattctg tctgttctgt tataacaaaa ctctcatact tctccatcag
     2401 tggttttaac atcattaact gttcataatg ccccccagag gaagccgcaa aacatatctt
     2461 tttgttcata ttaacctctc tatcagtcac gctgaaaaat atctctagtg taaacttttt
     2521 ctttaatatc atccaagcag gtatcatatc gattggcaat aatagaatca cacatcgcct
     2581 taaactgctc taaatcgttg ataaccttac ttccgaaaaa agtctctcca tctttcagtg
     2641 caggctcata aatcaccaca gtcgcacctt tagcttttac gcgcttcata actccctgga
     2701 ttgaactctg acgaaaatta tcactgttgc tcttcatggt cagccggtat ataccgatta
     2761 aaactggatt ttccctgttg acatcatact gattatattc agcgtagttg taatagcccg
     2821 ccatctgaag aacacgatct gcgatgaaat ccttacgggt acgattagat tcaacgatag
     2881 cctcaatcaa atttccagga acatctgcat agtttgccaa caactgcttg gtatcttttg
     2941 gcaagcaata acctccatat ccaaaggatg gattgttgta gtgagttcca atacgaggat
     3001 cgagacaaac accattgatt atctgctgag tattcagccc cttcatttca gcataagtgt
     3061 ctaattcatt gaaatagctg actcgcagtg ctaaataggt atttgcaaac agctttactg
     3121 cttcggcttc agtgaatccc ataaaaagag tatccacgtt ctccttaatt gcacttttct
     3181 gcagaagtgt tgcaaaaaca tgagcagcat ttaacaagcg ctcatcagtc atatcagtac
     3241 caacaataat acgagatgga tacagattat catataaagc cttgctctcg cgcagaaact
     3301 ctggactaaa aataatgttc ttactccccg ttgtctcacg gactgattca gtgtagccaa
     3361 caggaattgt agacttgatg accatgatgg tatcaggatt cacgtccatc acaacctcaa
     3421 taactgactc aacagcagaa gtatcgaaat gctgagtacg actgtcatag ttagtcggtg
     3481 tagcaataac aacgaaatct gcggatttat aagcgctcct tgcatccaaa gtagcagtta
     3541 agtcaagttc tttttctgta agatactttt caatatattc atcctgaatt ggagacttcc
     3601 gtttattaat cagttcaatc ttttcaggga taatgtctac cgcggttacc tggtgatgct
     3661 gcgccaatag tgtggcgata ctcaaaccaa cataaccggt gccggctact gcgatgttgt
     3721 actttttctt cacaatatct cctctttcct ctcttaagta tggagaatgt tactcctacg
     3781 gctattcatc atgtcaaaat aacacagctt agaatgttga taaaataatt ttccagaatg
     3841 tgataaaatc tctttcattt ataaaccaca catctaattt tatctattct ccacaagaca
     3901 tgtagagtat tc
//
LOCUS       DAZKNH010000151         3872 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000151 DAZKNH010000000
VERSION     DAZKNH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.155"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8MG_002581"
     CDS             complement(<1..132)
                     /locus_tag="ACG8MG_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0376572.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     "
     gene            207..485
                     /locus_tag="ACG8MG_002582"
                     /pseudo
     CDS             207..485
                     /locus_tag="ACG8MG_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
     gene            628..849
                     /locus_tag="ACG8MG_002583"
     CDS             628..849
                     /locus_tag="ACG8MG_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0376573.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            865..1707
                     /locus_tag="ACG8MG_002584"
     CDS             865..1707
                     /locus_tag="ACG8MG_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376574.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1770..3572
                     /locus_tag="ACG8MG_002585"
     CDS             1770..3572
                     /locus_tag="ACG8MG_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFQ0376575.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3631..>3872)
                     /locus_tag="ACG8MG_002586"
     CDS             complement(3631..>3872)
                     /locus_tag="ACG8MG_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0376576.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1374 a          562 c          774 g         1162 t
ORIGIN      
        1 atgagaaacg ttaatgccac gatcatataa gatttcttga acttcacgat agctaaggtt
       61 ataacgaaga tagtagccca cggctacaat aatcacatcc tgctgaaatt gctttccttt
      121 aaaatgattc atcgtcattc ctcctgctat ctttttctat tattctacct tatttgatag
      181 tagatttaaa actttgcaac agaaccaaag aactcatcaa gctggctaat aagggtcaag
      241 attatgaaca tctagcagat gagattgatg agctgagaga caagcgacag ctccttttag
      301 tagaagatgc ctccctcagt ggcgagaacg agcgaatcaa tgagctgatt gaatttatcc
      361 gcaagaacaa attccgtacc ttagagtacg atgataagct tgtaaggaag ataatccaga
      421 gcgttacagt ctatgaagaa cacttcgtca tatccttcaa atctggcatc gaaatggaaa
      481 tatgaaaacc agaagtaaat agcccatgat tctacagtag agtcgtgggt tttcttttgc
      541 ttgctgatta taataatatt ttaataacta ttgttattat aaaccaaatg atttataata
      601 ttcttagcgt aaacttggag gtgctgtatg actacggcag aaatgattaa agaactgtgt
      661 gagcaaatga atataagtgt ttccgaactt gctagacgta ttggacagac tccacagaat
      721 ttcaataaga aattacaacg agaaacggta accttagatg agttgaaagc gattgctgat
      781 gtgctaggtg tcaagtttgt gcaggctttt atattgcctg atggcaatga aataaagata
      841 ggtaattagg aggtgtaatt tgtattgaaa atcataacaa taaatgatgt tgagtatgct
      901 gtatttgctg ctaatgaagg tacttccaaa ccacaacctc atatcataga aacgaaatct
      961 ggtactattc cagaaggaaa acaactgagt ttattaaaag aatatcttaa gcagaatgat
     1021 atttccccaa taaaaggagc aacaacatat tggtgcattg acaaggttct taagcttgat
     1081 tcaagtaaag aaaaaactat aagtgaaaca atccataaac aaaaatactt atctttaact
     1141 gaagaaaata ttgagaaaca gcataagttt gtaggtgcaa gctctaatta tgggaaagaa
     1201 ggtctaataa tacatgatgt tttgaatgca tttcctcttc ataatgattt aaatactatt
     1261 gcgatgaaga ttgcggtaat tgatgtaaca aattcaacac atctttctca atataagagc
     1321 aggttgtctc tatatgattt ggctaaagta attctggaga ttccaaattt tgatgatcgg
     1381 ttggcaaaag gagatcctca gttgataaat attatcgcaa ggaacattgg tgcagttaat
     1441 atgttctcat ttgcatccaa gtactgtaca tatcataatg tggaagtgtg tgggagagat
     1501 gattactcca tctttgatgg tatcgttaaa aatactcttc cacactatat ccaaggacta
     1561 actacgaata agattgatac gtggcgaaga agttttgatt acgaggcatt taacgaatgt
     1621 gttggaaaac tacttgatga aaataatata catattcctt ttagaagaag aaagttagat
     1681 cacttcttgt ggtatgcgaa taggtagaat ataagaaaaa ggcatcgtaa aagtgattct
     1741 ggataaaatt aatgggggag gaaataatag tgggattatt tgattttcta aagaaaaaac
     1801 aaggaaaggt tagtaaacac gttgattatc aactggcttc tagtctattt cgtactggta
     1861 aagtttttgc tgaaaaattg gaagtggaca tatcaaatgt agatgagttt cgtaaaactt
     1921 ttatcgcttt tgatgtggaa acaactggcc taaattcttc ttcagataga attgtagaga
     1981 ttggtgcggt gctatttaaa gaaggagttc caattcaatc attcggaact cttgtcaatc
     2041 caaaagttag aattgcagcc tcagcttcgg caataaataa tataactaat gaaatgctaa
     2101 actctgctcc aaatgagcag gaagtttacc cccagttggt agaattttta ggagatgcac
     2161 tagatggaaa aatcactatg tgtgcacaca atgcaagttt cgattttgga tttttaagta
     2221 acacattttc tagacttggg tatgatgcta aaattaagta tgttgatact ctgagtctct
     2281 ctagaaaaca tataaaaggc attgtaaatc ataagcaaga gactgtagcg gaatatttcg
     2341 gattaataaa tgacactgcc catagagcgg aatcagatgc agatatatgc ggaaagattt
     2401 tttggggtgt tttagatatt attgaggagt cgttggaaga acaaaaaaag caaatagaaa
     2461 agatgactcc agacataaaa gaacttgaag cttgtgcttt tattcaagat attattgaga
     2521 aaaaaggatg cgacaccagt tggatcaggt atcgaaaaaa cagtaataat tatgtcgatg
     2581 taacttgtct atatacattc ttaaagttta agtttgctaa gaagggaaat tacatcatta
     2641 ttagaaaaga tgctacaaat ggaattgatt tacctataga accttgtact gtaagtgaag
     2701 gcggtacaac aaatgtgcga gtctattttc gtagcccctt tgacttaacg cctctttcaa
     2761 attatatttt cagtgcttat agcgattgtt ataattctat gaatgaatat atgagtgtta
     2821 gtaattacac taaaaaagaa gcagagcaaa gccttagtat gctgaaatct atttcaaaat
     2881 cagcgatgaa agagttatta ttcgatgcaa aaaatcgtgg atatgatgag actgaagtta
     2941 acctaaatat tgaaaaagtg atatctagag aagatgtaat tattaacggg ttgcataatc
     3001 gagtttcatt aaacaagata cgaaatatag ggaattggga gaaaggattt gatgcaggtt
     3061 atccatattg ggaacgtgga gaaactgcta ggaaaaatgg caaagtagat gaagctattg
     3121 cactttttga taaagctcga tataacggct atgatgctcc tgcgctatat gattcttatg
     3181 ctaaagctta tcgaaaaatt aaggattacg ataatgaaat tctcattctt gatgaaggaa
     3241 ttatgcgtaa aacacggcac gatgttggta ctttagaggc tcgtagggat aaggcaatta
     3301 agctattatt tgctaaacaa gaggcagagc ggatagctaa ggaaaaatct gattttctaa
     3361 aagcgaacaa aaaagaggat gcgattagtg agcctagcaa gaagcgtgga agacagatta
     3421 ttcaaatgac tgatgatgga acaataataa aagaattcga tactatagct tctgctgtaa
     3481 aagaagttgg cgttaactca aaaagtatta gggatgctgc aaatggagtt caaaaacatg
     3541 ctggaggata ctactggaaa tacaaagatt agcagataat ctaagataac ctaggatctt
     3601 cttaatagat acccgtggtg ctagttaatt ttaatatcct aaagaatatc tcaaagttcc
     3661 ttcaaaacgt cgttgtgctc gacttaatag aacactgtaa gccagtaata tgctttcaaa
     3721 tctactctct agccctttaa cagaacgtga gttgaaattt tgacaaccta atttttctaa
     3781 ggaagaaaaa acagtcccaa ctgtttttct ttgttttcca agtaagctgt ggtctagttt
     3841 ttctgattta tccatgtttt tacgtggcgg aa
//
LOCUS       DAZKNH010000152         3863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000152 DAZKNH010000000
VERSION     DAZKNH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.156"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(245..466)
                     /locus_tag="ACG8MG_002587"
     CDS             complement(245..466)
                     /locus_tag="ACG8MG_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376577.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(564..809)
                     /locus_tag="ACG8MG_002588"
     CDS             complement(564..809)
                     /locus_tag="ACG8MG_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376578.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1049..1663
                     /locus_tag="ACG8MG_002589"
                     /pseudo
     CDS             1049..1663
                     /locus_tag="ACG8MG_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2033..3034)
                     /locus_tag="ACG8MG_002590"
     CDS             complement(2033..3034)
                     /locus_tag="ACG8MG_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFQ0376579.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3193..3459)
                     /locus_tag="ACG8MG_002591"
     CDS             complement(3193..3459)
                     /locus_tag="ACG8MG_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0376580.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3449..3805)
                     /locus_tag="ACG8MG_002592"
     CDS             complement(3449..3805)
                     /locus_tag="ACG8MG_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0376581.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1431 t
ORIGIN      
        1 ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag
       61 ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt
      121 tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt
      181 gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga
      241 accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa
      301 atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt
      361 aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa
      421 ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc
      481 aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta
      541 tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg
      601 cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac
      661 tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg
      721 gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga
      781 acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat
      841 ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta
      901 ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa
      961 atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat
     1021 aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat
     1081 cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt
     1141 tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg aagctttaga
     1201 ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta
     1261 tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac
     1321 cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga
     1381 cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg
     1441 ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt
     1501 atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga
     1561 tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac
     1621 aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac
     1681 gtaattatta attagctttg tcatcaatac agacaatcat tttagattca tctagctaag
     1741 ttgattacat ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg
     1801 tccttattta aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat
     1861 aagatttgaa ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat
     1921 ttacgattgc acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca
     1981 ttaaaaaagt ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg
     2041 gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct
     2101 ccataataac agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt
     2161 cctctgaaat caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct
     2221 ttaaaattcc cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac
     2281 tccttaaatt gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg
     2341 aaaagataga attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa
     2401 attctattgg atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt
     2461 ttttgttaat tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt
     2521 ctaaattatt ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga
     2581 tattgtcttt gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct
     2641 taacttctat atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc
     2701 ctacctcgcc tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat
     2761 aattatcaac tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt
     2821 ctacaatttg tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg
     2881 cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat
     2941 tccatctata tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg
     3001 ctataaatac ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc
     3061 tttaaagtaa tttttgtata aaatttattg acttttacac tataggacaa cttttatcta
     3121 tttagtacaa aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt
     3181 ttcaactcgc ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc
     3241 atcaagttta tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt
     3301 tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata
     3361 aagagcaacc gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt
     3421 gtctttttgc ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa
     3481 gttcttttgc atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat
     3541 gggtaatgac ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc
     3601 ctaccttact tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt
     3661 gagctactag catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat
     3721 ctttttttgg taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa
     3781 atgtttcaaa agatattttt tccataccca attatatcat atattgattt tatattatag
     3841 tatataacat aaaaattcga att
//
LOCUS       DAZKNH010000153         3857 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000153 DAZKNH010000000
VERSION     DAZKNH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.157"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..350
                     /locus_tag="ACG8MG_002593"
     CDS             <1..350
                     /locus_tag="ACG8MG_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376582.1"
                     /translation="FIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLK
                     PPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFP
                     NKNNNFYFYRNKL"
     gene            complement(476..1153)
                     /gene="nth"
                     /locus_tag="ACG8MG_002594"
     CDS             complement(476..1153)
                     /gene="nth"
                     /locus_tag="ACG8MG_002594"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFQ0376583.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1160..1858)
                     /locus_tag="ACG8MG_002595"
     CDS             complement(1160..1858)
                     /locus_tag="ACG8MG_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0376584.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2126..3310
                     /locus_tag="ACG8MG_002596"
     CDS             2126..3310
                     /locus_tag="ACG8MG_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFQ0376585.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3316..3759)
                     /locus_tag="ACG8MG_002597"
     CDS             complement(3316..3759)
                     /locus_tag="ACG8MG_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0376586.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1002 a          761 c          636 g         1458 t
ORIGIN      
        1 catttattat tttttctgta cttttaagca ctactcttaa aggaaaattt atggaaagaa
       61 gctctcatgc aatgattctc acttttttat ggttgttgtt caccagaatc tatatttttc
      121 tgatgagcct gaaaccgccc aaatccatta gtttatgttt agttataaat ggtatttttt
      181 tattatcact tatcattttt attacacctt tagatagata cgtcacttat ttatataatc
      241 ctttggaata ctggacttat tttataggta tattggaagg cattattttc tacatcgggt
      301 attttcccaa taaaaataac aacttttatt tttatcgcaa taaattgtaa taagttacca
      361 tcctagctac tgagtatgta catttacttt ttcatgcaaa aaagataaac gaaacagctt
      421 ttctccataa taacaagata attcatacat ttatttgcaa ttaaagaaac cacgtctaca
      481 ggcgtggttt cttttttagc gccttttctt ttagacgcat ccgatttttc ccatcttgac
      541 atattgatag caaaggacat acctcgcact tgggatttct tgccgtgcaa tgatatctac
      601 caaaaaaaat cagtgtatga tgtgtcttta cccataattc ttgtggtact ttccgcatca
      661 gtgtttcttc tacttccatg accgtagcat ctagtttgca gatcctcagg cgttttgaga
      721 cacgttccac gtgggtatct actgcgattg ctggtatccc gaatgcatcc cccaatacga
      781 catttgctgt ctttctgcct acaccaggaa gagacatcaa ttcttctctt gacgttggca
      841 cttgcccatc gaaacgttca atcagctgct gtgcacacgc tttgatgttt tttgctttat
      901 ttcgatacaa accaatcgtt tttattttaa gaatgatttc atcgattgaa gcttctgcta
      961 acgcatcggg tgtcggaaaa gaggcaaaca aatctggcgt tgctttattc actgatacat
     1021 ctgtcgcctg ggcacttaag atcactgcaa tcaataactc aaatggatta ttgtgcttga
     1081 gttctccatg tgcttcggga aacattccat acatcgtttc tagagcttcc attgtttttt
     1141 gtttgcttaa catattccgc tactcctcca accaattttt cagtgagact ttcggcaatt
     1201 cttcttgttg agctgactgt tctacttctc tttgaagaag ctgctgtttt cttcgttttt
     1261 ggtcttcttc cacttgttgt tttgtacgta gattttttct ttcccacgat agcaaaatgc
     1321 ggtcaacgta attaaaacta taagcctgat ttaatacggc ttcccgcagt gctagcttca
     1381 aaatttccgg ctgataatgg tcttcttcta gccattgtcc gatccgctgg aactcgatcg
     1441 aagaaagcgg gcgtccaaat tcttgttcaa ataattgata cactgactgg atttcttttt
     1501 cttgtgtttt ttcttcgtat tttttttctt gtgtttttaa atactcacct aatagatcat
     1561 agatggggta aagattatat cgatctgctt ttttaccgtt gacaatcgtc gtttctatct
     1621 tgataaatcc atttgttacg agacgatcca gtatttgaaa aatctgatct tgtttgattc
     1681 ccatatccat agcaatcatc tgcaaatcag ggaaagtatc tccttcaagc tgtgccatat
     1741 gcaactgtag aacaaataaa aattcttcag attgtagacc gatcctatga taatttttaa
     1801 gtaaaagatt agatatgact gtttgtccag cgcttaagta attttctaaa ttcaacatga
     1861 tttctcctcc gctcaatcac agtatacgta aaaaaaacat gggaagcaag aacaggtttc
     1921 ttttctattt acttcactga tcggtcagat ttacaatgtc gtctacttga tttttgtttg
     1981 ttcatgtttt tgatgcgtat cttcactttt cggaataaat aaattaagta gatttcgctg
     2041 ttccttagaa tgttgacaag gaaacaaaat tactatacac tatttttgtt tccaagttaa
     2101 cattattgca taaaggagtt tttacatgta cagtttcaca aataaacatt ctaaagtttt
     2161 tggatacgtc tctgtcttat tcactggatt aggatttact atcgtcagtc ctgtacttcc
     2221 gtttttaacg ctgccttatt ctcactccat acatcagcaa gctttctata tcactttgct
     2281 catgtctgtt tatgctctag ctgccttctt atctgccccc attctcggta gcttaagtga
     2341 tcattttggg cgtcatccga tccttattat cagtttatta ggatcaagta tcggatattt
     2401 gctttttggt ttaggaaatt cgatatggat gttgttcctt ggtcgtatca ttgaaggatt
     2461 gacggcagga gaaatctcca ccttatatgc ttactttgcc gacatcacgg aacctaatga
     2521 aagaaccaaa gtttttggat ggatgggtgc tttagtcggt ataggaacaa cgctgggtcc
     2581 tattataggg ggtctattag ctgagttggg gaattctgtt cctatattta ttggagcact
     2641 ctttactttc ttaaatgccg tttatggcta cacatttatg ctagaaagcc ttccaataaa
     2701 aaaacgttca gtagatttaa gtttctccca tgtaagacct tttcaccaat tacaacaatt
     2761 attcaagatt tcatccgtta ttcctttatt gacagcaggt tttgctgttt ggttggcagc
     2821 tggctctctt caatctatct tttctcaatt ttcgattgac gcctttcagt ggaaggctgg
     2881 gttggttggt ttaagcttct ctttgattgg aatcttggat actgtctcgc aattgttcat
     2941 tatgcctcga ttgctcaaaa aattttcaga acagcagatc acaagaatag ggatgttcag
     3001 tgaaatcctc gcctatctat tcatcacatt atctggaatt cttctactgc cgattttgtt
     3061 tttatttggg attatctgtt atggtttcgg tgattctatt tttactcctg tattcaatgg
     3121 ccaactatcc aattctgtct cagataacca gcaaggtctt gtaatgggcg gtacccaaag
     3181 tatacaatct ctatcgcgcg tcatcggccc gctaatcgca ggacagcttt atgctgcagc
     3241 accatttctt ccgacggtat tcggattttt gcttatgatt tttgcctatt atcactacaa
     3301 taaaaaatag tagcttcatt tgctacttcg tataaattct ctgtatcgac cgataaaacg
     3361 aaatactgtg tcaaattctt cttttgacat attttcaaat acagtagcat ctcgctgtac
     3421 ccatttttga tggagttgct cgtgtctatt ccttattttt tgtccgattg gcgtcaattg
     3481 aaaatagatt tcttttttat tctcttcgct ttgataagtg tttatgatcc ctctagctat
     3541 cagttttttt gttagcttac tgatggctcc tctagtcatg taactagcat tcgctaattt
     3601 tgtcacgtta ggatcaggga ttttcccgat atgttcaatc acttctagtt catttagagt
     3661 aaatcctgca aattcttttt ccatttctgg tttattacga tgaataatct cattagctaa
     3721 atccagatat tcttctatta gcagttgtct tttatccatt tctacacctt ctttttcttt
     3781 cattatattg tttttttgtt tcctattcaa caaagataaa aatattttgt aggaaaaccc
     3841 aatctgaaac caagaaa
//
LOCUS       DAZKNH010000154         3785 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000154 DAZKNH010000000
VERSION     DAZKNH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.158"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            323..1816
                     /locus_tag="ACG8MG_002598"
     CDS             323..1816
                     /locus_tag="ACG8MG_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0376587.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            1951..2247
                     /locus_tag="ACG8MG_002599"
     CDS             1951..2247
                     /locus_tag="ACG8MG_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0376588.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            2348..3199
                     /locus_tag="ACG8MG_002600"
     CDS             2348..3199
                     /locus_tag="ACG8MG_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376589.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            3220..3432
                     /locus_tag="ACG8MG_002601"
     CDS             3220..3432
                     /locus_tag="ACG8MG_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376590.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1335 a          554 c          774 g         1122 t
ORIGIN      
        1 cagaaaaatt acataaaaaa aagagacgga aataattgat tttagcagaa tcaattattt
       61 ctgaatcaca tggttaggac atgtgttcac gctcgtctca agaatatgta agtagctacc
      121 aactacttat attaaaagtt gatcggatac cttttccgat tgcctttatt ctaccaaaaa
      181 aaggcgagca actcaacaga agaacgttta tttagtgtac ttaaattaaa tattcttaaa
      241 cactgaagag agaacacata ctatggctga gattaaagct aaactgtgtt ctctttttgt
      301 ttgagaaagg acgttacaat cgatgttgag cgaaatttat tcagctattc ttaaaaacgg
      361 cttacgtaca tacaaatatc gaaacagcac catgaaacct gcaggtcata ttgattccag
      421 taggagtggt gctatctttg cgtaccgtag caagcaatta atgaatgttg ggcgtggtat
      481 ggtcattact tcggaagaag ctattttaga gaacgaaaag aaactaacgc attggacacc
      541 taatacctat cgctttggta cttatgcgga tgattatcga acagtggtta aaggtcattc
      601 tgagaaaaac ttaagtcaaa tcaacacatt tgtagttgat atcgattcaa aagagaacca
      661 tcaaggcgaa attattttag cctgtttaga tcaagtgggc tacatgccaa cgctaatttt
      721 agaatcagat catggttatc aagtttattt tgtactaaag acaccaggct atgtcacaaa
      781 gaaaagcaat ttcaaggtga tcgatgtagc aaaaaaaatt tcaaaaacaa ttcgtctaca
      841 gttggctgaa aaattgtccg gagtggatct aggatgtaac cacttcggaa ttgctcgatt
      901 cccacaagaa aataatattg tttttaaaga aatgaactat cagtattctt ttgctgactg
      961 gattcaatgg tcgatgaagc ttgaatcaga tcaaaaaaca aaagaagatc gagaacgaaa
     1021 actaattgtc tttcctgaga aaaaagaaat tcgtcaagta gatgaaccgt ggttcgatat
     1081 gttacttcat aaagcaaaca tcgtcggcgg agaaggacgt cttggtcgaa ataatgtgat
     1141 tttcacgctt tctttagctt attactcttc aggttatgca ttggatactt gcgaatacaa
     1201 tatgttcaca ttcaacgaac ggttacagga accgctatct gaagcagaat tagtaaagat
     1261 tgtacgtagt gcttattctg gtaattatca agcagcaagc agagaattca ttattgaatt
     1321 gtgccagcaa tgggtttcac caaccttaaa aaataatgat ctgttcattc aacgacgtgg
     1381 ctggtggaaa tttaaaaagc cacgagatca aagaaaatat agtcataaaa atgaatggaa
     1441 agaagatcta ttggcttatc taagcgaaaa aagtcttgct agtgagcctt atttgaacct
     1501 gtcaaaaaaa gaattggctt cacaactaac gatgcccaag cgaacacttg ataagatctt
     1561 aaatgagtta cgagaggaga ataaagtctt ctacaaggta acacgaggaa gaaatggcgg
     1621 aatgatcatt gcttcagtca aaacgttgtt cgcgagggtt attcaactaa gtaaagaaaa
     1681 gagactagat tattcaacaa ggatcacaga ggtatttgga ctcaacgaga gcttagtaga
     1741 agagacattt agacaattat caaccccctt gaaccctctt aaagaagtga atctttttga
     1801 agtagataca ggttgaaaaa taaaaggtgc aaattgccct atacaatata tctatgatac
     1861 ttctgtaggt gttgctccct ttgcctagct acgtgtaggt ggtatactta taactgcgta
     1921 tgcagtctta tgtatcatgg aggaagttgt atgtcgttaa aaaattatgt ctattcacat
     1981 cctgttaatg tgtttatcat tggtcgtttg ggattatctg tggaagtgtt ctgtgagctt
     2041 tatggtttta agcaggggac tttgtcttct tgggttacca gggaaaaaac agttgcttct
     2101 ttgccgattg agtttcttca tgggttgtcc ttagcttctg gggagactat ggatcaggta
     2161 tatgattgtt tgtgtgtgtt agagcaggag tatattgagt ataaaatagc taacgagtta
     2221 agaaaaagga agaagtatat ccagtagtag tttttattta cttaaaaaat tggttcattg
     2281 agcaacctat cgctgctaaa gtagcaaagg aaaacgttaa aggaaagaat ttagggaaga
     2341 aatcgaaatg aaatcggacg actggattga acacacctgg cttcctctga aaagaaatag
     2401 caaacgaaag aaattttggg tattgcaatt atggccatta ggaaaacttt gtagttacgg
     2461 ggcacgactt attcttgatt taaccgttta ctcgattgga gtatttggaa cggcttatta
     2521 cttgataaat gatcccaata ggattgcagc atcagacatt tttacagtgc taatgacgtt
     2581 gatttttgtc atgctattgt ctatttttac tcaattagtc atgcaattta ccaggcaaaa
     2641 attaacagca ggaaaacttt tcaaaagcct attcgattta attgttgtaa atgcaattct
     2701 agcgacttta ataaaacaaa ttgaacaatc tggaagaaca acaaaaggag tgaaggattt
     2761 tttattgacg tttattcact ttgtaaatgg acacatgtat ttgtttgaag tgggtattat
     2821 tctctttttt gtcgggtatg tatccacgaa acttattaaa cgttcaattg aaaaagaaat
     2881 tgaaattgac ctgattccga cacttcttgg ttatgagtac aatgttgatt ttggaagata
     2941 tgacgaattg atcaatacgg atatcgttca cacgagtatc catgaatcta ctgttaaggg
     3001 catcagatct attttaaaaa atggccagga aatgaaagca atagatcgaa gaaaagagat
     3061 ctacaatttt tatgaagtcg aagcaagcct acgatatggc atcggaaaat tgtggttaga
     3121 tggaagtaat attcgtcaaa acaaaccata cattttttgg aacagtagtg atttagaact
     3181 tccaatgaaa gataaataat tatggagaat cgtttgaaaa tgattcctct atacaggatt
     3241 gtgcttatag ttggcatgga gttaaaaacc ttaaagaagg agcagttatt caaagtgaaa
     3301 gaacgtcata atcagttact atgtagtaaa acgggtacga tgaacattga ataccgaaca
     3361 gaatatttag aattaacgaa tcaaactgat tttttgttga ttcgaaagca acaagagcaa
     3421 gaaaaaaatt aatggctact tatagccaat tgattgatga atgggaacaa ttcaaggaat
     3481 ctaaaattcc agaacgtctc taaaagcctt tatttgtaaa gagcataagg aaacgtggga
     3541 acacttttta aaaaaagtgt tccaataggc tgtgcctatt acgtttctca aaaataaatg
     3601 tgggaacaaa atatttaaaa ataggatagt gaaaggatag tgaaggatag taaaaggagg
     3661 gtagtaggat agcaaaaagg ataggctcct tttcgctatc ctttttaaat tggaggaatt
     3721 aatatggaag taagaataag aaatgtggat gagaaaatcg taagagaaat tgatcgaaga
     3781 tgtga
//
LOCUS       DAZKNH010000155         3541 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000155 DAZKNH010000000
VERSION     DAZKNH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.159"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(267..971)
                     /locus_tag="ACG8MG_002602"
     CDS             complement(267..971)
                     /locus_tag="ACG8MG_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376591.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1158..1403)
                     /locus_tag="ACG8MG_002603"
     CDS             complement(1158..1403)
                     /locus_tag="ACG8MG_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376592.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1420..2271)
                     /locus_tag="ACG8MG_002604"
     CDS             complement(1420..2271)
                     /locus_tag="ACG8MG_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0376593.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2437..3441
                     /locus_tag="ACG8MG_002605"
     CDS             2437..3441
                     /locus_tag="ACG8MG_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFQ0376594.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1098 a          604 c          687 g         1152 t
ORIGIN      
        1 cttttttctc tatcagaaaa tatgtttatt attagaattt tttatagtta aggttaggca
       61 tcttaagaaa tcaggcaact ccaagatatc ttcaggtaga agagaagtcg cctaacattg
      121 taggatcata ggtgatttgt tggacaggtc aagctgatat ttgagcctga tgttagaagg
      181 aatttctttt tacttgctgt agaaattatt atatgactaa aaaggctggg attttaaatc
      241 ccagcctttt atgacaaata gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt
      301 tcgatcaaag gaatcaaagt caatttgact ttgtatttcc gttcgagtgc tttttcgata
      361 acgaactcat tttcagtagt catagaaata gttgctaatt tacgaggttc atcagcattt
      421 ttttcattaa taactacttc atgttcattt tgttctgtgt aaaatttagg atactcagca
      481 acggcttttg aaaaatcagc aaaacttaaa gcggcattcg tatcagttga gcgtgtattt
      541 tcagttgtgt ccgcttttgg tttttccgtt ttttcttctg gcacactgac tgtttcttct
      601 gtaactggtg tcgcaacagc ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc
      661 cagtaagaca cgatttcaat aagaagattg tcacggaatc gttcgaaaat cccgttttct
      721 cctgaacgaa ttctctttaa tacagtattg aagttgtcat cgattggcac gttgatccgt
      781 gtatcttcat ttgttttccc agctaatccg atcgttaaga tttgacggtt tttgggatca
      841 agcatgactt gtaacggcaa accataacgt cgcaagctct cttcttctag agaacggaat
      901 aattgtcctc ttacaccatc caatgaagga cggaattgat ttaataattt ttgtttctgt
      961 tttttcttca tatgttctct ccttcgctca taccctttcg attataagaa aaaaacagcc
     1021 aaatgaaaag gagtaatgca aaaaaacttg cgtatacgca aattacaaca gaaaaatttg
     1081 cgtattttcc cgttttttga aagaaaaata aaaaatggaa cggattatgt atatatacat
     1141 aatccgttcc attctttcta ttttttcggt atcaaggcaa aaacaggtag ttcaggtgaa
     1201 taaatgactt catgttcgtg ccagggtttg accattagcg aatgatcgaa ccgttttttc
     1261 agttcttcga tgatttgatc atgtgtccag tttcttgcag gaaaatgttg gattttctct
     1321 gcatcgatca aaaagacaaa atcggaatga aaagtctggg taacttcttc cgagagagaa
     1381 ttcaagcgtt cgattggatc cataaaatca cctttcgact taaaattggg tagtatctgg
     1441 gtcttttgct ttgccgacta caccatctaa ctgatcgatt tgtttcattt cttcttctgt
     1501 taattcaaaa tcaaataatt cagtattttc tttgattctg cttggtgtca ctgacttagg
     1561 aagaggaagg aattcgtgtt gcagcgacca acgcaaagca acttgtgcaa tcgtcttgtc
     1621 atgtgcttca gcgatttgtt tcatttcagg cacatcgaag attttaccag tgcctaatgg
     1681 actatatgct tcgaggatca tatcatgttt ttttgcatac tcaactacag ttgtctgtaa
     1741 ttcgcctggt gcaaggaaaa cttgattgac catcggcata atttttgctg tttttgctag
     1801 tgtatctaga tggtggggta agaaattgct cacaccgatt gctttgattt tacctgcttc
     1861 atacagttct tcaaatgctc gccatgtatc tgcatttgct tgttcccaat tatcacgaaa
     1921 agcaacaggg tttggccagt ggatcaaaaa cagatctaaa taatctgttt ggagcttctt
     1981 caatgattct tcaaaagaag acatcacaag ttcatagctg tggtttgcat tccataattt
     2041 tgttgtcaag aatatctctt ctctaggaat tccactttcc ttaatcgctt ggccgacgct
     2101 ttcttcattc ttgtatcctt gggcagtatc gatatggcga taacctgcag ccagcgcttc
     2161 tttaacagag gaaacagcga catccccatc aggtgtttgc catgtgccga atcctactac
     2221 cgggatttcg taaccatttg atagacgata agtgcttgtt aatgaattca tactttcagc
     2281 tcctttttta ttcgttcaac tataagtgta gcgcattttt cgaaaaaaag aatccatttt
     2341 gtttctactc ttgaaatttt tgtttaaaag aaaatgattt tgattctggt atttttgatt
     2401 ttttttccgt aaactaagac agaagggaat gagaaaatgg atatctattt aaaaaaagca
     2461 attctgcata ttattgacag agaaactggt tctccagtct tttcacaaaa agagctggat
     2521 ctgacaaaag aatatattcg tgattttctt caaaagaaga tccaaaaaat ctcttctgct
     2581 caaacaaaaa ctggacaact agcagaagaa agcatgatcg ttgaatcact taaaatcgct
     2641 caagaaaatt ttgtggaagc aagtgaagca atcgttcagc ggtggttcga tatctaccaa
     2701 gaaagtgaag aagcacctag tgcagatgta tttgttgtct tgtatgaatt agatacagtg
     2761 atgtacttat ctttattgaa agtcaattat cgtgaggctt acactcactt tgttgaagca
     2821 gacgaagcag ggatcgacaa ccgcttgatt ttgaatcgag caattctagg ggcgaaaaca
     2881 caaaaagcag atgaagcatt cgctgtgaat ttgactgatt ggacctacga attaattgaa
     2941 aaaaaatatg agttttctgg caaaaaagag ctttattttt ctggaagagt gatcgaaagt
     3001 gttcctgcac cttctttaga agaaaatgta aaagtgatcc aaaaagtagc caagcatctt
     3061 ggaaaaaaat ttgagacaga agaatttgat atcatggctg atttgaaaga agctgtctat
     3121 gatacgatag aagaaaaagg tcggttggat catgagatga ttgcggaaaa agtcttcaag
     3181 gaaaacatca cggcaaaaat ggcttttaaa gaagaagtac aagagcaagg atttgtccca
     3241 gaagcacctc ctgtaaaaga agtacaagaa atttctgaga aaaaatttgg aaaacaaaaa
     3301 ttcaagctat caaacgggat cgaactgatc gtaccagtag atgtatatcg caacccagat
     3361 ttgattgaat ttgtgaataa tccggatggt acgatatccg tgatgatcaa aaatgtggat
     3421 gaagtattga atcgtttata aaaatgacta gaatattagg tgttcaagta ccagtgatca
     3481 gttaaatgaa ttgtgtcaag agaaggatcg gaaaagacga atataacaaa aaaagagcgt
     3541 g
//
LOCUS       DAZKNH010000156         3473 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000156 DAZKNH010000000
VERSION     DAZKNH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.160"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2177)
                     /locus_tag="ACG8MG_002606"
     CDS             complement(<1..2177)
                     /locus_tag="ACG8MG_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0376595.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="ACG8MG_002607"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="ACG8MG_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          986 a          763 c          541 g         1183 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatagtatt tatattagtc
     3301 atactgcttt ttaacacagc aaattccaga aaaacattcg ttacatattt gtgaattttt
     3361 ccaaaaaata acatttttat aaaacgacga aaaataaatc actaccagtc aacttctata
     3421 atattaccaa ataacaaact aactatccat caataacgtg ttttattttt taa
//
LOCUS       DAZKNH010000157         3337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000157 DAZKNH010000000
VERSION     DAZKNH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.161"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            733..1065
                     /gene="traE"
                     /locus_tag="ACG8MG_002608"
     CDS             733..1065
                     /gene="traE"
                     /locus_tag="ACG8MG_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFQ0376596.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1684..2025
                     /locus_tag="ACG8MG_002609"
     CDS             1684..2025
                     /locus_tag="ACG8MG_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0376597.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            complement(2262..>3337)
                     /locus_tag="ACG8MG_002610"
     CDS             complement(2262..>3337)
                     /locus_tag="ACG8MG_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0376598.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         1085 a          491 c          677 g         1084 t
ORIGIN      
        1 taatagctat tttaatagaa tttataagtt aaaatattat gataaaaatc acaaacgtag
       61 ccttttacgt cataagtgtt aaaagaaata attaatttca aatatagaaa agggaacgta
      121 tgtttgcata ctcctttttt ttgagttata ataatacgcg aagagagata atttcattat
      181 ttaaacaagc taaaaatctt gatattactt cttttcaggc aatacgaata ttattcgctt
      241 acattttttg tataaattta attgtctgct atgcttgtga tgaattgatt ttcgttttgc
      301 acttttggat gataaatttg gacaattgaa aatacaaaaa aaagaaaggc ggtatgaact
      361 cggaaggctg gtaaccttcg tcagcgttca tacacggcat taagatttgg cccttaatgc
      421 gttaaccttt cagtgttacg gtattacccg caatggattt agtataacag atattcaaaa
      481 gtgataccac tttttttggc tgtgttaagg ttaaaaatac gtgatattac gatgaatcta
      541 tgcctaagca taatgttata aaaatgtccg tgtggctctt tgccgtatga cgaacgctga
      601 aattagaggg aacgcatgtt tggatgcgtt ccctcttttt tgtttgtcat gaacgaaaat
      661 caattctcta tttttgaaaa ataacaagaa aaacttgtta aaaatattta aagaaattgg
      721 gtggtttatt ttatgtcata tacaggaatt ttgagtctca aagatatttg tcactacggt
      781 aaacggtgta ccgctacaga gaaaatcacg aagaaactat caactggcca aagtaaaacc
      841 gttgtgcagt gtaagaaata tataatccag aaagacaaag tctcagagga aatgatttat
      901 tacattggga aacaaaagca aataatttta aaagatccta tccccttaaa agagctatat
      961 ccaacaatca aacatgttta tgaccaaaac ggtgtactga ttggtcgaag aaaaaatgga
     1021 gtcttgcgtt gtaccgcaaa agggatgggg cgtttgatcg gctgaattga tcattctcat
     1081 aacttgtatt ttatcgtttt tcgcttgctt gaaaatattg tcgagacttc taaaggaact
     1141 tagagtcact agaaaaataa ggagtagact tgatctatca acaattccct tcggggagtg
     1201 acggggtagg ttttaacgat tggtaacgta aaaaattggt caatcgagta aatgcgcttg
     1261 tttggatagt ggtgaaatga atttgtgtaa ataataagcg cagagtaaac aagagggaat
     1321 ttagaagtta gacaagttag aatagcgtct agattaatca tgagcagcta ctaagggatc
     1381 tagactttga cgcaaactca tggaataagg agtaggtctt ctcgtaatgg ggaggacgca
     1441 caagaaattg tgtcgtgtgc tgcagtacat gtagatactt gcgaggacgt tgattattgg
     1501 atcaatcgag catttaccgc tgtctactag ggtttctcgt ttcggacgcc ataaacgagt
     1561 cgaaattttt tatgtatttt taagtaaacg aaaaacgata aaatatgaac aagtcctgaa
     1621 taaagtgaaa aaattaatat aaagcataga gaaaatgaaa tttacgtaat ggaggaatga
     1681 caaatgaaaa attatgatcc gaatatacga ttgggcacac atactattaa ggtttctttc
     1741 caacgttggg attacaaagg atttgtgacg tttagaaggg gcggaaattg taaaggatta
     1801 gatgttttag cattagacga ggatgattta tacgatcaaa cattgactga taatccaatt
     1861 ggattcggct tgctacctga agatgatgaa ggtaatgaat ggtttaaaat gacgcttatg
     1921 aatgataatg gagacgaatt atctgttgaa gatacttggt catatttgag tgattatatt
     1981 gtcagctttg aaatcattga atttgtagcg gataaagaag aataaaaact ggaggaataa
     2041 attttacaga taaaggctta gaaggagtca cgacgattaa taaaaaggta gtattacctt
     2101 aggcattgag taaggaattt atttagtcta atggtgataa gtcaagatga aatttaagat
     2161 tttctttatt gagtaatatg ttttgaagcg cacgaaacta gacccgatca aaatcaaatt
     2221 tttttcgggt tgattcaagg atttgcgtca ataacggatt gttatttctc tatcacactc
     2281 cttggtggcg tatagagttg gtgtttacgt agtagcgtat ccaccagacg cgtaaatttt
     2341 cttgcggtta ggacgagtgc acgtttgtgt tgatgctttg gaacctcctt atatttactt
     2401 tgataaaaag ctttgtattc aggaagataa ttttttacag agttggcagc ttcaactaag
     2461 tagtatctaa aatatcgatt tccttgtttt gtcagaggtg tattttgcga ctgatagttt
     2521 ccagattggt tcactttcca acttaatcca gcgtattttg ccaattttgt ctgatcttca
     2581 aatctttcga tttgaccaat ttcagcgatt aaaccggcag catagacttt accaacgcct
     2641 ggaatactgg ttaagcattg atattctgga atgacaacaa ttacttgttc gatggcttta
     2701 tctaattctt tgatattttt ttcaagtgct cgtatttctc gtacgagaac acttaaaaca
     2761 acattaattg attcttgtgc aagagcacct aggcggtaac tcatggtaat tgcacgttga
     2821 attgctttag cgattttttc tggttgcttg aaacggcctt ttcctttttc ttgaagtaaa
     2881 tcacagaaag cttctaaagg aagctcagca agttcatcca atgtgaaatc ttccgtcatg
     2941 agtgaaatga gggttgcact aaaaagactc gtcgattcgt ctcgcatttc tcgagcaagc
     3001 gtattacatt tgtacgttag attttcaaga aaatgttgct ttgtacgaat caattgctta
     3061 ataagttgat agcgagtacg tgttaaacgc tgtaaggcca tgtatttctc ttctttgata
     3121 ggggaagttg tgaatcgttg aatacgcaag aaatcagcga ttcttaatgc atcgtttcga
     3181 tcagttttat tctcatcaaa catgcgacta aattgtttca cccgaaaagg attttcaatc
     3241 gttacaagtg tgttgagctt ctttagtttc tcatcttcat gaaaaaacat ggaaggatgg
     3301 aagctgtaca tggacgttga ctccatgcca atcacaa
//
LOCUS       DAZKNH010000158         3251 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000158 DAZKNH010000000
VERSION     DAZKNH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.162"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..280
                     /locus_tag="ACG8MG_002611"
     CDS             68..280
                     /locus_tag="ACG8MG_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376599.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(558..2357)
                     /locus_tag="ACG8MG_002612"
     CDS             complement(558..2357)
                     /locus_tag="ACG8MG_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFQ0376600.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2481..3077)
                     /locus_tag="ACG8MG_002613"
     CDS             complement(2481..3077)
                     /locus_tag="ACG8MG_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0376601.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1166 a          591 c          577 g          917 t
ORIGIN      
        1 tacaaggtaa tttgttatta taaaataaag aaaaaacaaa ataaaaataa agaaaagagg
       61 gaatgaaatg gtaaaagcag aagtaggaag atcttatata tgcaaaccga tcggagtatc
      121 tcatgaacta atcggctgta ttgaacgtac atatatcaac acggctgtcg tgtatgttga
      181 aagttatgac caacgagact atcaacttat caatgaaagc aagtatcgca tgctcgtaaa
      241 attctgtgat attagcgcac ctgctgaatt ggtttcttaa acagtatttt tgagaattcg
      301 caagaactcc cttataatat aaataaagac agaagggagc gagaagatat gccaaaaaga
      361 aaaagttcag ggaaaatggg aattattctt gcagtgatct ctgcaattcc tgctatttta
      421 gctattgttc gtgcgatgaa ggaatcaaga gaaagttaag cacacattat gagcgtcaaa
      481 caaaaaacgc atatacctgc ggtggcaggt tatgcgtttt ttgttgtata aagacttttt
      541 tcattaacga tttttgatta gccgactccc aaaacaaaat ttagcagttt atctgaataa
      601 gtgatttctt catctgtttg tttcacactt tcatttagta gtcccatctc atcggttatg
      661 ataccatcca caccataaaa catcatgcga ctcatagcat ctgaatcgtt gattgtccat
      721 acatatactt cttttccgtc ttggtgggca gaattaatga agttacggtt gatcgtacta
      781 tattccattg tcagaaaatc agcaggcgtc acagggggcc caacaatatt gaaaggtaag
      841 atgtagccca cataaaagtt tggttctgct tcttttaaat ctgaaacggt ttggtacgtc
      901 aaactttgca aaatatgatg gttctctaag ataactttgc ggtatttatg gacaaatcgt
      961 tcgacaaggt catccgaatc ttttttagag gtcttgatct caataagtaa tttttggttt
     1021 agcttatgtg ctttttccaa ataagaatca aaagaacaaa caggggcttg cactccgttt
     1081 tcagtaactg taagatttgt caattctttc aatgtaagat cctgtggtgt tccatttacc
     1141 cctgttaagt gacgaagatt tgcatcatgc atcactacga aatgaccatc ttttgtttcc
     1201 tgaacatcca tctctacata atcaggatga tagtctctgc ttgttttttc aagtgcttct
     1261 aaagtatttt gcgccccatt ttgggtactc acacctctgt gagagacagt cactggtttt
     1321 gtctgaacag ctgaagtcag ataatccata ttgtaaagac agacgccaat accgaaaaaa
     1381 actgcaaaaa gagtcaaacc agtgtttttc agcgcagaga aacgcaagtt gggggcttgt
     1441 gggataaacc acttagggat ctcaggcaag aagccttcgt catccatgaa atcaacaatg
     1501 atataaaaaa tccctacggt agacatcacg atattgaata ataaaatccc ttgtaaaagc
     1561 gtcatagcaa acacagcgct gatcaatgca tagtctggga aaaattgttc aaccatactt
     1621 tgtgccaaaa taacaacgat atatcctaaa gaagaaagga gtaaaatcgt accgccaatc
     1681 acaataaatt ggcccgtgat ggccaaaaga cgcgatttgg tcaatgacca gctttcccga
     1741 atggctgctc gaaaaggacg atctcgtaga atcatttccg gtaaagcaaa aatcagtcga
     1801 atcccaatat agccaaggaa aatataaacg agtaaaaagg aactgacgat gatccaacga
     1861 ttagcaaaaa taaaatccat aatgaaagcg gggattttta tttttgacag tagatcagaa
     1921 ttaaaactta atccgctgat cggtagaatc aataaaaaat aagcaaggaa aaacaaaaaa
     1981 gtaatgggtc tcactttctt cagctgaagt atcgtcaagt gtaagagttg cttcaaagag
     2041 attggttctt tctttttaat aaaaaaaaca ctcatcaaaa gaaaagtaaa ttcgaaataa
     2101 actaacagta aaatcgtcat caaaactacg attaacgaga gtagaacccc aggatgttga
     2161 gaaaaaattg aaggaatatt gtcataagac aaatagtcaa ttgcccctcg gtgtaaaata
     2221 aaacgggtcg tactgctcaa taagggtaaa caaataaata aaataaagcc atgcatcagc
     2281 aagacatcac ggaaataagc ttgtgttcct tttaaaaagt cccaagcatt acctaagcta
     2341 cgtttcaaat aattcatcct atcacctgct cttcacattg tagcgcaaat ctaatgaaaa
     2401 aggaaagggt gcagtgtgtt ttgcagaccc tttcccagat ttaacattgt caatcaatga
     2461 ctaattttac aaaataaata ttatccaaga agaccactta gatattgacc tccataaatc
     2521 aaggccatac tatataagaa aatcgacagt ggtttagcaa tcaaaatgat tagtgtatat
     2581 cgtttcactg acatgtttgt caatccagcc atcaaacata acgcatcgtc aggagcaact
     2641 ggaagaaaaa tagctagagc aaacagcttc tcaaaacggt tttgattatc cagccagcca
     2701 atatatttgt tatatgtctt atcgctaatc atggaaagta tgaatggctt tccataacga
     2761 cgccctagca gaaaaatgat aatcgaacca atgcagattc caatatagtt atagataaat
     2821 ccagcataag ggccaaatat caggacacca gctgcagtac tgatccctcc aggaatgatt
     2881 ggaataacta cttgcaggat ttggatgaag acaaaaataa tcggtcctaa aatgatggaa
     2941 tcacctacta atcctcttaa cgcattcaaa tctttgaaaa cacctaaatt aataaaataa
     3001 atcgtcagcg cgattgatag gcctaatccg ataatggata tgaaatttat cagttttcga
     3061 gaggcagcaa tattcattat tcatcaactc cttagaggtt ctttgttaac tatactttac
     3121 tagaaaagca actaactgcg ccattttttg atatgacaga aaacaaacaa ttttgtaagt
     3181 tcatcataag tataaaagat tgatctcaaa aaaacatcca actaaagact tatttaataa
     3241 cttgtttttt t
//
LOCUS       DAZKNH010000159         3248 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000159 DAZKNH010000000
VERSION     DAZKNH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.163"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..684
                     /locus_tag="ACG8MG_002614"
     CDS             <1..684
                     /locus_tag="ACG8MG_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0376602.1"
                     /translation="KNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVNSAENAW
                     TNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGN
                     IKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVV
                     NGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAG
                     AVGGTLYAT"
     gene            674..1414
                     /locus_tag="ACG8MG_002615"
     CDS             674..1414
                     /locus_tag="ACG8MG_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0376603.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1411..>3248
                     /locus_tag="ACG8MG_002616"
     CDS             1411..>3248
                     /locus_tag="ACG8MG_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0376604.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         1221 a          395 c          629 g         1003 t
ORIGIN      
        1 aaaaatatta aagatacggc atacaatacg tggatttcta taaaaaatac catgattaat
       61 acatggaata acattaagga ctctttctgg aatattgtta ctggaattgt taattctgct
      121 gaaaatgcat ggacaaatct aaaaaatggt gtttcaaatg ctattaatcg ggtaaaagaa
      181 atctttaatt cgttaaggga agtcaattta tttgaaattg gcaagaacat cattgatgga
      241 cttataaatg gtgtaaaaga aaagtggaat gctttgaaag atactatcaa aggtattgca
      301 ggtaatatca aaaattggat taaaggagct ttagatattc attctccatc aagatggatg
      361 cgtgatatgg ttggtaaaaa tattgttcaa ggtattatcg ttggtattga taaagagcaa
      421 agtaagttag atcaaaccat gacaaattta gtaaaaacac cgtctgttca acctgtagta
      481 aatggaataa gtcacctacc aattacacaa acaaagcaaa atatatctgc tagtgaaaca
      541 aaagaaattc atttacattt aaatgtttat ggagatttac ctgattcaat ggttcggaag
      601 attaccacaa aaattaaaac agaattgaca agacaaatga aacgagatgc tggtgcagta
      661 ggaggaacat tatatgcaac ttaagagagg acaatttttt attaatcaac attattcttc
      721 tgaatttaat gtgtatattc aaaacaggcc tgcttctgtt tcagctagtc gagtaattga
      781 attgagagag cgagaaggaa atgattctat cattattgat aaaggctatt acaaaaatgt
      841 gactagaaag attgagtgtt actataaagc accttcaatt gatatggtgc aggagtggga
      901 agatcgaatc actgaatggt tagatatgtc gtcttatagt gattttattc tttactatga
      961 tgaacaatat atttatcaag ctgtagtaat agaaggacct gaatttaaag gaacaagaaa
     1021 aacaggaaat attgtccctt ttgaatttac agtgagtatt cgaccattta aggaaaatta
     1081 caatggtaga tttactattc gacaaacaga aacttttgaa atttataacc cagagaagta
     1141 ttcttcaaaa ccgcttatta aattgagtgg ttctggagat gcttcttttt atattaataa
     1201 ggataaatat gatttgaaat cgttggacag agaattattt atagattcta aattagaaga
     1261 ggcttatcga aaattagatg gtaatttaga acatcaggat caggtcacct tatttttaga
     1321 ttttccattt ttatatcctg gaaaaaatga aattaaatgg acgaaaaata ttcattcatt
     1381 tgaaataatg cctaggtggt ggagaaaagt atgaaaccaa gaatatatag tcctactgaa
     1441 acagatttct caacgaatgg cttaggaatt ttaaaggatt gtacaagatg tggaatatat
     1501 gaagtagcta atggaaaata tgaattagag ttggattatc ctttaggaac tagatttgat
     1561 gaatattttg aaaatgacta tcaaataaaa gcaaagccaa acgatcaaga agagtatcat
     1621 atctttttta ttgatgataa agatatagat acttttttaa atacagtaac tatttatgct
     1681 cagagtcgta caaatcgact tggaagacgg gtagtcactc ttgtggaagt ggactctaaa
     1741 actggtcaag aagcgatatc aattattgaa actaaaatgg ataaaaaatc tgacatacga
     1801 ctttattctg atattacggc tgtttctagc acaatctttg aagcgagaaa tgttttaaat
     1861 tgtattgctg gtgaacaagg atcattgctt caatattggg gcggagaaat taaacgtgaa
     1921 ccatttaagc tttctttgtt aaagcgaaga ggtcgcaata atgttggaac gattcggtat
     1981 ggaaaagata tgtctggttt gaaggtcaaa ttagattgga caggtataaa aacaagaatt
     2041 attccatatg ctgatcctca aagtgaagta ggtacgacta atcgaattta tggttcgcca
     2101 gtagatagtg cctatattaa taactatcct gatgtctata cagaacatgt tcagtttaca
     2161 gaagaacaag gagtaaaaga cattaagagc ttaaataaaa tagctaaaaa ttactttaaa
     2221 acgatcaatc ctggctgtga taagcctaaa gtttctatta cggttgaatt tgataagttg
     2281 actgatagtg aagaagcgaa agaatttgcg aagattagaa attatggtct gtttgataca
     2341 tttaaaatat atcataaaaa atataatatt tatcttgaat caaaggttag tggactacaa
     2401 tacgattctt tatctgaaaa aactttgaaa ttagaagcag gagacattca agttgctttt
     2461 tatcagcaac aagctgtaac tattcaggat aaattaaaag attatgcaac gaataattat
     2521 atgagtgatt ttaatgatta tgtttcttca atgattacag gtcaaggcag tgcaggagga
     2581 tatgtagttt tatggccaaa agaaaaacct tccaacatct ttattatgga taatccagat
     2641 ttagaaaagg caagagaagt actaagaatg aataaaaatg ggattgcttt ttctaagaaa
     2701 ggatggcggg ggccttttaa ttcagcttgg acattagata gtatatttaa tgccaatttt
     2761 atacaaactg gattaatcaa agcagatatc tttcaaaatt cttttaacaa gactggagat
     2821 gtattgaaat tagttaatgg actactccaa atttggaaca acaaaaagaa aataatggag
     2881 ttaactaaaa aaggaatgga attttggaat tctaagggtt caattggaac gattggaaca
     2941 actgattctg ttggtaatcc ttttcctggg gcttctactc caacccctat tgaagataat
     3001 tctttagtta ttcgtacaaa tggagacggc aaatatattt tgatttctcc taaagtagga
     3061 aaaggattag ttttattagg aaatggtaaa gcaatttatt ttggagactt agatgtacaa
     3121 ggaaaactta cagttaaagg aaaagaaata attcctggac aaggtggtgg tccatcggga
     3181 ggtggagaat ctactggtgg gtatcctagt gaattaaaaa cagatgcaga aaagagagct
     3241 tggagaat
//
LOCUS       DAZKNH010000160         3126 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000160 DAZKNH010000000
VERSION     DAZKNH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.164"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1040
                     /locus_tag="ACG8MG_002617"
     CDS             72..1040
                     /locus_tag="ACG8MG_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0376605.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            1050..1538
                     /locus_tag="ACG8MG_002618"
     CDS             1050..1538
                     /locus_tag="ACG8MG_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0376606.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            1834..2115
                     /locus_tag="ACG8MG_002619"
     CDS             1834..2115
                     /locus_tag="ACG8MG_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376607.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            2117..2533
                     /locus_tag="ACG8MG_002620"
     CDS             2117..2533
                     /locus_tag="ACG8MG_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376608.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         1251 a          543 c          475 g          857 t
ORIGIN      
        1 attagggggt tgacccccat tagaaagctc tttataattt tgttaagtag ttaatcttga
       61 aggaggattt tatggcaaat gaaattgtga aataccacca tgaattaaat acgattcctt
      121 tacgcaaatt tacgtcagta gaaatgaatt tattcttctc tataatatct agaatgcgtg
      181 atgtaggcga taaaaaagta caatttacat tcgaacaatt aaaagattta agcaattata
      241 aagcaactgc aaatgttcga ttcatagatg atttagaaac aacgtatgac aaattaatgg
      301 atttacgttt tggtagaaga agtgctgacg gtttgcaacg tgaacgattt gttttattta
      361 atcaatttaa aatcgatgga aaagctgata ttccttttgc tgaaatacaa gtccatgaga
      421 aagccttacc cttacttaat aatcttgaag aatgggttcg atattctctc caacaattca
      481 atgaattaga aagcagttat tccaaaacca tgtttagatt attaaaacaa tacagaacca
      541 aaggaattgc caatttttca aaagaagact ttcatgaatt acttgatatc cctaaaagtt
      601 atagagaaag tgatataaat aaaaaaatta taaagcctat tcgccaagaa ctaaccgcaa
      661 tcttcaaagg tttgacaatc caaaagagat acggaaaagg acggggaaag cctgtcattg
      721 gctatcaatt tacatttaaa cctgagataa aaaaatcaga cgactttaac aaacatgctc
      781 caaccaaaga agaacaaaat aaagctttgg aactagctaa tcaacaggtt caatctgaaa
      841 aagaacctac ttcattacaa gaaaaaatca aagaacaaaa acgaaaatta gaacttttac
      901 aacagttagc agaattagaa agaaaacaaa ataccccaag taagaaaatt aacaaacaaa
      961 tagcagataa caattccgat attaccccag aatttaaaga aaacttacta gataatctcc
     1021 aaaacttatt taaagaataa ggggttttta tgacagataa aactatcaaa gaattagcag
     1081 atattctagg tgtttcaaaa gataaagtga agtatcaact gaggaaatta cctagtaatt
     1141 ttacctacaa aaaaggaaat attacctacc tgaaaaaaga agcagtaaca acaattacaa
     1201 aggttttatc aggtaatgaa tcgggtaact tacctagtga attacccgat tcattaccta
     1261 ccgattacct actcaaggag attgaaacta agaataatca tattgaagaa aaagataggc
     1321 aaattgaaaa acttcaaata gctttagaaa cgcagcaaaa attactcgac cagcaacaac
     1381 aactcaccct acaagccaat caacaaatca aaaaacttca cgaacaactc cagctaacct
     1441 atgcagaaga atcttcagaa aaagaacccc tttctgttac tgaagaaact caaaaaggac
     1501 aaaacaagga gaaaaaatgg tggcaattct ggcaataacc caacaatttt tattcataaa
     1561 aattgttggg ttactttctt tttttgcttt tacaagcata gcgacatgat ctcacgtcgc
     1621 tattttttaa agagcataag gaaacgcggt atcactttct tacgaaaggt gctaggtagg
     1681 ctgtgcctac cacgtttcta agaaaaaaat ttgctatcac tttttcgtca ttgtggatag
     1741 cttatgtata gtgtttcggc aaaaaacagc tatacaaata ctatatttgt atgatataca
     1801 taaattatac acaaatgcga aaggaattct tctatggaca ttttagttcg gaatattgat
     1861 ccaaaagtta tttcagaaat agatagacgt tgccaagaac tcacaacaaa aactggcaaa
     1921 aaatggagcc gtaatgcgta cctaaaagtt ctaatcgaaa acgattttga ccaggcactt
     1981 aggcattaca aaaaagatca attcgattta ttactcgaaa aatttatcga tatacaagca
     2041 aaaaatactg aaacattgaa cgactatgtt catacaaaca accaactcat tgaaacactt
     2101 atcggaggaa tataacatga gaaaaacttc tgttcaaaaa cacgttaccc tttatcccag
     2161 tcacattgac gttttagatt ccatcatcga aaaaaatcca gatatcaata actactcgga
     2221 agccattcgt tttctatgta tgagttatga acctaatact aatacgacaa ctaccagcaa
     2281 ccaagcaaaa ctaaatgcga tgggaaaaga aatttctatt atgtccgaaa tcattgcaga
     2341 atttgctgat ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtata
     2401 tcaagaagtg aaagaaaaaa ttgaaaatca actcaatccc tcccgacgaa atcaacccaa
     2461 aaatcgtcaa aaagaaacca atcaaacatt cacttctaga aattttgacc caacaaactt
     2521 tctaaaaaga tgaaaaagaa accgaaaatt ttttctattt ctcagtttct tttttagggg
     2581 gcagtcttcc agcaagattt gcaagttacc ggtaacttgc acaaaaaaag aaaaatccgc
     2641 ttgtgcgaat ccttcttttt gcattttggg gcgctgcccc aatctccaaa tatcggttat
     2701 ttaatagcgt ggtaactcaa aaaaatagaa cgtactctga aataaaaaaa gactgctcta
     2761 acgagcagcc aataaaaaat aatgagcgtt tattcttgaa aaagcagcgc gcttatgaag
     2821 cgctgccaaa aatttaccat attttatgaa aaataacaag tttctaaaaa ggaatcggtt
     2881 aagattcaca aagaatcgta aacagacaat agcggaaaat caagcttccg aacgtacctt
     2941 ttagctagac caatgaaaaa tcacaaagtg agcccctgta tcggttgata caagggctac
     3001 ttggcatact cgacattatg tctactagct cgataaataa cgattatgtt cccaaaacaa
     3061 tattttatga actataacaa aaatttgtta tcgcatattg tatattttaa ttataggtgt
     3121 atatca
//
LOCUS       DAZKNH010000161         3061 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000161 DAZKNH010000000
VERSION     DAZKNH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.165"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(278..892)
                     /locus_tag="ACG8MG_002621"
     CDS             complement(278..892)
                     /locus_tag="ACG8MG_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0376609.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1498..1875)
                     /locus_tag="ACG8MG_002622"
     CDS             complement(1498..1875)
                     /locus_tag="ACG8MG_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376610.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2175..3056)
                     /gene="galU"
                     /locus_tag="ACG8MG_002623"
     CDS             complement(2175..3056)
                     /gene="galU"
                     /locus_tag="ACG8MG_002623"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0376611.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          921 a          643 c          471 g         1026 t
ORIGIN      
        1 tccatatgga ttctttcttg tctctttttc cactactttt tatttttctg acactacata
       61 ttcttataac tcatttctca taactgtgaa acaacttata aaaataacaa actttaaaca
      121 aaacaagctt aaaatcaaag atgatccatc ttaaaataac taaaacaaaa aaagtagtcg
      181 ttgttaatcc tcattaataa tcgttttctt tttttcaaac gctccatttt tatgtggaac
      241 attatttttt taaggaactt tgtcaacatg tggataacta ttttccactt gtgtcctgga
      301 ctagctgttt tgctgtaaaa tacaaaactt gctgcagcaa aaagatcgtt gcaagagagg
      361 ctgttccgca gatcgctgga tctagatttg gcaaagtcaa gactgcatca tgtaaattgg
      421 cgtggttgtc caaaaccaaa tcagcaaatt caaataatcg caaaccactg ctatgttgcg
      481 atttgatact tcgtgacaaa tcaaatccag taacaatgat taatggatga ccattttctt
      541 tgatccactg agctagttcg atgattgccg ggtttcttcc ttcattcgaa agaagaagaa
      601 aaatgtcttc tgatttgaca gaaagctgat caataattgc ctgtcctgca ccttctagcg
      661 tttcatagac acctccggct ggattttcaa tcaatatact tggtacaaga tccggggttt
      721 gatcccaaaa ttcaaatgct acaccactta gtgtgcggct agcatatatc tgcaatctac
      781 caccttgatt aatttgtttc gcgatccaag tgccagcttg aagcatcagc ggctcttcta
      841 tccttaattt ctcttccaat tggtgataat agtcaaaata acataaactc atttatctct
      901 cccgcctagc tatacttgac atgaatagaa acagctgcct aatctaagga agtacataaa
      961 aaaacagtag ttagacagta acacatgtgg tctttgctgt ttcaaacaca aaaaaggcat
     1021 tgaacgaata cgcccaatac cttgacatag caaataaaac aacgtgcaga ccatggaaaa
     1081 tatctacata ggggtagaat tttcacccta gattaggtac aataaattag gtcgatgcac
     1141 tgcattgact atggcaaaat gatcggtcag ggtgtatgtg tgtgactggt cattccctga
     1201 ttcttagcgc caactaaggg tcagggagcc tttttttatt cagctgtttc tatctgaatt
     1261 tattgtaagt aatctcttat ccaagtgcaa gcaagaaaat acaaaaaaac attggtatga
     1321 tttatatata tataaataac ataaaaaaag gaatggctta tcccgataca acaagcattc
     1381 ctttaaaaaa gattctttta tataagtgaa aaaagctttt ttccaccaat taaataaatc
     1441 tctcatctca atagtttatc cattatttta tctttttttg cgtataccta ctaaatatta
     1501 ccgtcttctt ctcgttcttc tactttgttc gagagaatct gtactagaaa aagtagttgt
     1561 atgtctacct tttctgccta tccgagactt tcttttcata tatgggatat aaatgattag
     1621 atacagtaca gcaaaaaata aagcaaatac gcaagcttcc caactaccta acgccaattc
     1681 aaccatcaag gtagcaaccc caacggatgc tgcataccag ataaaaatca gaaacgtttc
     1741 tttttttctg accatgcgaa acatgataaa tagactaata agcaatagtg gcaacacaaa
     1801 tatccaattg cttccttcca tcagccactt gagtatatca agcagcgact gtgcaacagc
     1861 gactaatttt cccatagtga acacacacct ttttttataa ttaaaaaatt cgccttttta
     1921 ttctactatg aatcttctgg cttggctttc atttctctta tgtaatcatt ttcttttata
     1981 caacttaaaa aaaaattaaa ccattagcat aatattttct gaatctattc acgaaagtct
     2041 ttttctatct ttcgttttcc acgactcaac tgatctagag tcaaaaaact aaaacgacag
     2101 ttgcgcaaat gacgcacaag ctgccgcttt aataacaaat agcctttcat tctcagcagg
     2161 ccacttattt ccagttaact ttctgttctt agctttttac tcaactggat aatatatgca
     2221 cttaatgaag tcccaatttc cgaatgttga agtccatatt caatatttgt cttcatatat
     2281 cctaacttat cgcctacgtc gtatcttttt cctttaaact ctctcgcata gaccgcctct
     2341 gtctggttaa gagtctgaag tgcatccgtt agctgtatct cgtttcctgc tcctggttct
     2401 tgagtttcta atatctcaaa tattttagga gtcagtagat aacgtccgat aatggctaaa
     2461 ttacttggtg cttttcccat ttcaggtttt tctacaaacc cttttactct gcaacgaccc
     2521 ttttcgctat ctgttttagg atcaataatt ccgtatttag atgtttcatt ttctggtaca
     2581 cgcatgacgg caacagcgga agcttgtgtt tcttcataat ccatcatcaa ttgttgagtc
     2641 aatgggatat catctttcat caaatcatcc cccagcatga cgacaaacgg ttcatttcct
     2701 ataaatgctt ttgcttgaag cacggcatcc cccagtcctc ttggatgaga ttgacggatg
     2761 aagtgaagat tgacttgagt cgtttcttca actaaagcga gtaattctgt tttttccttt
     2821 tcccgcaaat tgttttccaa ttcgatattt gcatcaaagt gatcttcaat gggtcttttt
     2881 tcttttcccg ttacgatcaa gatgtcttcg attcccgaat ccaatgcttc ttccacaata
     2941 aactggatcg ttggtttatc cacgattgga agcatttctt ttgccatggc ttttgttgcc
     3001 ggcaaaaaac gtgttcctag cccagcggca ggaatgactg cttttttgac cttcacttgc
     3061 t
//
LOCUS       DAZKNH010000162         3038 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000162 DAZKNH010000000
VERSION     DAZKNH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.166"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG8MG_002624"
     CDS             complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG8MG_002624"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFQ0376612.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1034..1954)
                     /locus_tag="ACG8MG_002625"
     CDS             complement(1034..1954)
                     /locus_tag="ACG8MG_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0376613.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2067..2819
                     /locus_tag="ACG8MG_002626"
     CDS             2067..2819
                     /locus_tag="ACG8MG_002626"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFQ0376614.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          856 a          612 c          526 g         1044 t
ORIGIN      
        1 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
       61 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      121 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      181 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      241 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      301 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      361 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      421 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      481 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      541 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      601 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
      661 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
      721 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
      781 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
      841 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
      901 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
      961 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1021 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1081 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1141 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1201 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1261 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1321 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1381 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1441 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1501 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1561 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1621 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     1681 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     1741 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     1801 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     1861 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     1921 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     1981 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2041 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2101 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2161 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2221 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2281 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2341 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2401 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2461 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2521 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2581 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     2641 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     2701 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     2761 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     2821 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     2881 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     2941 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3001 ctcatatttt tcgtttggtt gggcttatta ccgcagat
//
LOCUS       DAZKNH010000163         2967 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000163 DAZKNH010000000
VERSION     DAZKNH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.167"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(510..1076)
                     /locus_tag="ACG8MG_002627"
     CDS             complement(510..1076)
                     /locus_tag="ACG8MG_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0376615.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1076..2032)
                     /locus_tag="ACG8MG_002628"
     CDS             complement(1076..2032)
                     /locus_tag="ACG8MG_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFQ0376616.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2032..2688)
                     /locus_tag="ACG8MG_002629"
     CDS             complement(2032..2688)
                     /locus_tag="ACG8MG_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0376617.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          931 a          581 c          531 g          924 t
ORIGIN      
        1 tcttttttac tgctttgatc ataaaagtct gcatattcat cactgatatt ttttctaata
       61 aattcttcca tcataaaaat cacctcaaaa cgattatacg aacttacgtt cttgttgtaa
      121 agcgatattt gaggggcaaa aaaggggcat aatttgactg atattaagtt agctattgat
      181 atatgaatga catacaaaaa tataaaatct tttaaaaatg cttatatatc aatgtttttg
      241 ccattctttt atatatctgt tatatgtcaa aaaaatgtgt tcaatttcaa tttgtatttg
      301 aataaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac
      361 agcttctcat gtttttgatt tggttggact tattaccgca gatataagct tttgagcacc
      421 gtgaataaag aggttatgaa atcaacgctc taacctgagt attagagaaa aaaacgcaaa
      481 acatatcatt tatgttttgc gttttttatt tatctttttt tctcaataca atataaaata
      541 ggcggctggt tcttttggtt gataaaccga taattcagca cattatatgt ttcctgtggc
      601 agtttggaac aaaaagaatc aaccatatcc agttcttttt ctccaccttc atgaccatag
      661 tagacgacaa ggatcaatcg tccgcgaggc acaagtcgtt tcaatatttc ttccatagct
      721 gtgcgtgtag tctctggcat agtgatgatt gctttatcac tttttgggag ataccctaga
      781 ttgaaaatcc ctgctttgag attttgttgt tcagctatca cttctcctaa cgtttcgtgg
      841 ccttgtagaa acaatgagac acgttcttct aactgacgct gttctagcct ttcacgagta
      901 gcatcgattg cttgttgctg gatatcaaaa ctatagacat gaccagtttt tccgatgtgt
      961 tctgctagaa aaaccgtatc gtggccattg cccatcgttg catctacgac gtgatcccct
     1021 ggttgcaaga tttcttgcag taatgtatgg ctgaaatgca aagcagtttg caacatcaga
     1081 cgctcacctt tttccgtaca tcatattttc cttggaagct atctctgcga agaagttctt
     1141 catcaattgc attcaagact tcccattttt tcaagctcca cattggtcca atcaaggaat
     1201 cccacggcgc atcccctgtc aaacgatgga tgatgatctc ttttggtatc atttccagct
     1261 ggtcgcaaat gacattgaca tagtcaggac ggctcattaa ttgaaggcga ccttcatgat
     1321 agtcccgcaa cattctcgtt ttgcgcatca gatgcatcaa atggagcttg attccttgga
     1381 tatctgaatc taaaatggtg cgtctgacat tttctttcat catttctaaa ctttcgcctg
     1441 gtaatccgtt gatcaaatgc gtacaaacac ggatattatg tttgcgtaat ttagccacgc
     1501 catctaaata agtttgataa tcatgtgctc ggttgatcag ttttgaagtt tcttcaaaag
     1561 ttgtttgcaa tccaagttct acccataaat acatccgctc gttcagttca gcaaggtaat
     1621 caactacttc atctggcaga caatccggtc gtgtaccgac tgataatcct acgacacctg
     1681 gtaaattgac tacttgctca aatcgttctt tgattatttc aactggcgca tgggtgtttg
     1741 taaaattttg gaaataaacg atgtattgtt ggacatgagg ccatttttga tgcatcatgt
     1801 cgatttcttt ctggaattgg attggaagag ggtcttcagg tgcaacgatc atgtctcccg
     1861 aaccagatac actgcaaaaa gtacaaccgc catgggcaac agtcccatct cgattcggac
     1921 agtcaaatcc tccatcgatc ggcaccttga aaattttccc gccaaactca tttcgtaaag
     1981 cgtaattcca agaatgatat cttttatttc cttctgaata aggaaaagtc attaaaagtc
     2041 cttctttctg taagagttat ctatttgttt tcttttttct ctatacactg gataagtcag
     2101 gcacagccaa gaaagactga gtaagtaacc gccgatgata tcagtaggaa aatgaacgcc
     2161 cagataaatg cggcttatcc cgacacttac tataataaac atcatcagca attgaagcaa
     2221 tgtgcgtaag gttctttttt gaacaaaagc tggaagcaga aatagtaaag aaccgtaaag
     2281 aatcatactg cctgctgcat gtccactagg gaaactgtaa cttgtttcaa ctactaagtg
     2341 cgccaatgaa ggacgctctc gcatgaaaaa tagtttgatc accgggttga tgattccagc
     2401 aattacaata aatccagaaa cgaaccaaaa tgtttctgcc ctttttttct tataccacat
     2461 ccataaagcc gcacaagcaa ataaaatgat gacggtaaga ggattaccga atttagtcat
     2521 aaagagttgg tagctgttca gatcaggata agaagctctg acaatgcctg taaaccactg
     2581 atcgaaacca gaaagccacg acagatggaa ctttacgatc cagcccaaaa tgaaaaacag
     2641 gacaagaaag aagctaccag ccacctgaaa tgatgtttta tatttcatac aattcctcct
     2701 ttgaagcaat gacttattat agcatttttg gtccagcgaa aaaaacggta aagagataaa
     2761 accaatgtat gattcctctt tgaataacca atattcttat aaaaaaaata actgtaatcg
     2821 gaaagacatt ttttggtaat gcaaatgaaa tgttagggaa ataaaatcag tgtttttcga
     2881 aatcctttta aacaagaacg tagacgtagg aagaacttga aaatgaacag aagttgttca
     2941 ttacggtaaa ttacaatatg attacaa
//
LOCUS       DAZKNH010000164         2966 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000164 DAZKNH010000000
VERSION     DAZKNH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.168"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            231..1556
                     /locus_tag="ACG8MG_002630"
     CDS             231..1556
                     /locus_tag="ACG8MG_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0376618.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1549..1899
                     /locus_tag="ACG8MG_002631"
     CDS             1549..1899
                     /locus_tag="ACG8MG_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376619.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1881..2066
                     /locus_tag="ACG8MG_002632"
     CDS             1881..2066
                     /locus_tag="ACG8MG_002632"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376620.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
BASE COUNT         1062 a          462 c          555 g          887 t
ORIGIN      
        1 ttttctaata taactaagca aaactggata atacgacact tattttcaaa ttttcacaag
       61 actaatagta aatatccatt taataaagtg atacaatgtg ttgattttac aaatcaacag
      121 aagaagtagg gaaataactt acctatgctt ctttttttgt acatttaatc gattcttctt
      181 tacaaaaacg aacgtatgtt tgtataatgt ggaagaatgg agtgttattt atgatggatt
      241 attcaaaaga gccagtaaat gattactttt tgatcgatat gaaaagcttt tacgcaagtg
      301 tcgaatgtat agaaaggaat ttagatccat taacgactga attagtcgtc atgagtcgag
      361 cggataatac aggatcaggg ttgattttag cttcttcacc agctgcaaag aagaagtacg
      421 gtatttctaa tgtcagtcga ccacgagatc taccgcaacc ttttcctaaa acgttacatg
      481 tcgttccacc acgcatgaat ctctatatta agcgaaatat gcaggtaaac aatattttca
      541 gaaggtatgt agcagacgaa gatttattga tttactcgat cgatgaatcg atcttaaaag
      601 tgaccagatc aatgaatctt tttacgactg aaggaacaag aagtcaacgg agaaagaagc
      661 tggctcaaat gatccaggag aatattaaag aagagctagg attgcttgct acagtaggtg
      721 tcggagataa tcccttatta gctaaacttg ctttagacaa tgaagcaaaa cataatgatg
      781 gattccttgc tgaatggacg tatcaaaatg ttcctgataa agtatggaat attcctgaaa
      841 tgacagacct ttggggtatt ggatctcgaa tgaaaaaaag actcaaccaa attggtatca
      901 tgagtattaa agacttagct aattggaatc catacactat taagagccat ttaggtgtga
      961 ttggcttaca gctgtatttt catgcgaacg ggatcgatcg aacagatata gcgatcccac
     1021 cagaaccgac aaaggaaaaa tcatatggta attcacaagt gttaccacga gattatacca
     1081 ggagaaatga aatcgaactg gtggtaaaag aaatggcgga acaagtagct attcgtatca
     1141 ggcaacataa ctgtaaaaca ggttgtgtcc atttatacat tggcacgtcg atcttagaaa
     1201 cgagaccagg tttttcacac cagatgaaga tccctataac agataataca aaggaattgc
     1261 agaattactg tcttttcttg tttgataagt attatgaagg acaagaagta aggcacgttg
     1321 gaataaccta tagcaagctt gtgtacactg attcgcttca gctggattta tttagtgatc
     1381 cacaaaagca aattgacgaa gaaaatttag ataaaattat tgacaagatt cggcaaaaat
     1441 atggttttac gtcgattgta catgcttctt ctatgttaga aggcgctcgt agtattacta
     1501 gaagtacatt ggttggtggc catgcaggcg gcgcaggagg gcttaaaaat gattgattat
     1561 aatgatcgtg gacgtttaaa gtgggcaggt ttttatttat ctgatcatac tgaaaaaatt
     1621 gatgccgaca atgaacaacg agccaatcaa aatttggcaa aagaacaaat gacgactgaa
     1681 gaggttgcgg aagtattgaa cgctgcagtc ataaaaaata gatcagtatg tattcaaaaa
     1741 gaagaacgaa acgctgaagg atactatcca gaagatattg ttagcaagat cttaggtagt
     1801 gatgagctgg ggatctacgt tggaaaaaac aaagttcact acgacgaaat acgtcatatc
     1861 gaattttacc aggataataa atggggtcga ttagaatgaa cattatccag caatatgagc
     1921 taaagtatat aacctttgat cagctatcag aagagatttg gggttatggc cagcgtttga
     1981 taaatgaggt tggtgttgaa cgcttctctt tctatgtaga agctgcagca ggataccata
     2041 aatacgaata ctatattggc tattaaagtg caataaaatt atatttcttt tatgttttac
     2101 attttgtatc aattctgata aaattaccta taggtaatta aaaaatataa aaaaactagt
     2161 tacaacctta ggacgttgta actagcttga agcatactag agctaactaa aatagcacta
     2221 gaaagcaatg ttaaacccgt ccaacaaact tgatttaaca ttgagaccag cttattcagc
     2281 taaatatagt tgaataagct tcttttgttt taaaaaaaca aaaggtgtac tacgaataac
     2341 gcaagtaaaa gaaatactat tacaaccagt aggctacaag taagtattac taaacattgc
     2401 gtagttagaa cctttgctct attgtctaaa gccacaaaat tagagatcag ctttatgatg
     2461 ctaagagcga gctgcatcca tgcggacata ggttcttttg acactattaa ttctcctttt
     2521 taggataagg gaaattgcta gtagtttttt cattagcacc atcccccttc atttgattga
     2581 gtctagtgct gctgtgatag tttgctgttt aatatgctta cattcttatt ataacataaa
     2641 tgaaacaaac acatggttag aggtcgtatc aatcaaaata gtgtctcaca aacgatttat
     2701 tacttactcc tgctgactaa cggactgttc ttcagtaact gtaactatat gtttgttaaa
     2761 accaccatta cctgtagata aaaaccaata tatttactat actcactata ttaaatatat
     2821 ttagtatagt aaaaactaga aacaataaaa tttgtgaatg ttgtataccc ccaaacaata
     2881 cacatatttt gaaggattag gattaataaa gctaaaaata tagtaagtat actaaatata
     2941 tttactatat ctagtatact tactat
//
LOCUS       DAZKNH010000165         2929 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000165 DAZKNH010000000
VERSION     DAZKNH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.169"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..981
                     /locus_tag="ACG8MG_002633"
     CDS             85..981
                     /locus_tag="ACG8MG_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0376621.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1073..1288
                     /locus_tag="ACG8MG_002634"
     CDS             1073..1288
                     /locus_tag="ACG8MG_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0376622.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1306..1578
                     /locus_tag="ACG8MG_002635"
     CDS             1306..1578
                     /locus_tag="ACG8MG_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0376623.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1580..2443
                     /locus_tag="ACG8MG_002636"
     CDS             1580..2443
                     /locus_tag="ACG8MG_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0376624.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1160 a          464 c          510 g          795 t
ORIGIN      
        1 gagggctttt atatattaat cacaaatcac ttatcacaaa tcacaagtga ttaatcacaa
       61 atcacttgtg atttgtgatt cttaatgata caatattact atacaaaaaa agaatggggc
      121 gtagttatgg agaaggaaaa actaaaaata cttgaagaat taagacgtat tttaaacaat
      181 aaaaatgaag caattattat cttgaataat tactttaaag gtggtgttgg aaagtccaaa
      241 ttatcgacta tgtttgctta cttgacagac aaattgaatt taaaagtttt aatgatcgat
      301 aaggacttac aggcaacatt gacaaaagac ttagcaaaaa cttttgaggt agaattgcca
      361 cgtgtcaatt tttatgaagg cttgaaaaat ggaaacttgg cttcttctat tgttcatttg
      421 actgataatt tagacttgat ccctggcacg tttgatttga tgttactgcc aaaattaact
      481 cgctcatgga cttttgaaaa tgaaagtaga ttgcttgcta ctcttttagc acctttaaaa
      541 agtgactatg atctcattat tattgatact gtaccaacgc caagcgttta tacaaataat
      601 gcaatcgtgg cgagtgatta cgttatgatc cctttacaag cagaagaaga aagtacaaac
      661 aacattcaaa actatatttc ctatttgatt gatttacaag aacaatttaa ccctggacta
      721 gatatgatcg gttttgttcc ttatttagtt gatacggaca gcgcaacgat aaaatcaaac
      781 ctggaagaac tgtacaagca acataaagaa gataacttgg ttttccaaaa tattatcaag
      841 cgaagtaata aagtaagtac ctggtctaaa aatggaatta cagaacacaa aggctatgac
      901 aaaaaagttt tatccatgta tgagaacgta ttttttgaaa tgcttgagcg aattattcaa
      961 ttagaaaacg aaaaagaata gaatcacaaa tcacaagtga ttaatcacaa atcacttgtg
     1021 atttgtgatt gttgatgata aaataagaat aagaagaaat agaaagaagt gagtgattgt
     1081 gggaaattta ggcgcacaaa aagcaaaacg aaatgataca ccaatcagtg caaaaaaaga
     1141 tataatggga gataagacgg ttcgtgttcg tgctgactta caccatatta taaaaattga
     1201 aacagcaaaa aatggcggaa acgtaaaaga agttatggat caagccttag aagaatatat
     1261 acggaaatat ttacctgata aactttaaaa aggagtgaaa atgaaatggc agttacgtat
     1321 gaaaaaacat ttgaaataga gatcattaac gaattatcgg caagcgttta taatcgagta
     1381 ttaaactatg ttttgaacca tgaactagat actaaaaata ctcgtttact agaagtgaat
     1441 cttttaaatc aattagaagt ggcacaagaa gttgatttat ttcaacaacc atttgaagaa
     1501 ttacaagcta ttcatgagta ttggcggtca atgaatcaat attcaaaaca aattttgaca
     1561 aaagagaaag tggcttaaca tggcgaatat tactgacttc accgaaaagc aatttgaaga
     1621 tcgtttagaa aagaatgttg aacgactaac taaaaataga ctagcggttg aatcgccaac
     1681 cgctttttta cttggtgggc aaccaggatc aggtaaaact agtttgcgat cggcaatttc
     1741 cgaagagaca caaggaaatg ttgttatcat tgataatgat acgttcaaac aacagcaccc
     1801 taattttgat gaactagtga aactttatga aaaagacgta gtaaaatacg ttacccctta
     1861 ttctaatcgc atgacagaag cgatcataag ccgtttgaga gataaagggt ataatttagt
     1921 gattgaaggt acaggacgaa caacagacgt tcctattcaa accgcaacaa tgcttcaagc
     1981 caaagattat gaaacaaaaa tgtatgtcat ggcagtacct aaaatcaact catatttagg
     2041 aacaattgaa cggtatgaaa ctatgtatgc agatgatcca atgacagcca gggcaacacc
     2101 aaaacaagcg catgatattg ttgtcaaaaa cttaccgacc aatttagaaa cccttcataa
     2161 aacgggctta tttagcgata taaggcttta caatagagaa ggagtgaaac tctattcaag
     2221 cttagaaacg ccttccatta gtccgaaaga gaccttagaa agagaattaa atcgtaaagt
     2281 atcagggaaa gaaattcaac cgactttgga gagaatagag caaaaaatgg ttcaaaatca
     2341 acaccaagaa acccctgaat ttaaagcaat tcaacaaaaa atggaaagct tgcagccacc
     2401 tacaccacca atacccaaaa cacctaaact tccagggatt taaaccttaa aaaagtaaaa
     2461 gagtagttac caaaaaacgg taactactct ttttttataa aaacatttcc tcatttttta
     2521 aaagctgatc gagaaataaa gcacacaact gtattctttc ttttttcagt tggtacattt
     2581 tgcagtctgt aacgtgccat ttactgtttg ttaccttaac atatccagtc gaaaacacaa
     2641 gggtatataa acgagcaagc tcgcccttgt gttttctttg tgtctatgtt gcgttctcgt
     2701 cacagcaaac gacacgaaca cactggcgaa aatgaaccaa caaaacgaaa aaagaaagga
     2761 gcgtacactg taatttattt ctagctttta aaacatgagg aaaacagctt cacttcctca
     2821 aacgcctaac aaaaatggtt gtcaaaaaac taaaaatcaa aaaaagaaaa gaggaaaaga
     2881 aaatggaaaa aacaaaaaaa ttacaattag aagattttac agaaaatgg
//
LOCUS       DAZKNH010000166         2842 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000166 DAZKNH010000000
VERSION     DAZKNH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.170"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            58..873
                     /locus_tag="ACG8MG_002637"
     CDS             58..873
                     /locus_tag="ACG8MG_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0376625.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            999..1256
                     /locus_tag="ACG8MG_002638"
     CDS             999..1256
                     /locus_tag="ACG8MG_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376626.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1508..2080)
                     /locus_tag="ACG8MG_002639"
     CDS             complement(1508..2080)
                     /locus_tag="ACG8MG_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0376627.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2096..2701)
                     /gene="fic"
                     /locus_tag="ACG8MG_002640"
     CDS             complement(2096..2701)
                     /gene="fic"
                     /locus_tag="ACG8MG_002640"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFQ0376628.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          920 a          523 c          443 g          956 t
ORIGIN      
        1 gactacaagt aaaaaatact gtatgatcat gacagattca agaaaggagc aataagattg
       61 gttaagatag caggactttc taaaggtgga aaaaccgttt cacaacttta taaacaaaac
      121 tacagagact ggagacagtt cagaaaaaac attaaggatt cttactttat tctaccaaca
      181 gaacttgaaa aatatttacc gaataacaaa gctattaatt tatatctgta ttactgtttt
      241 aaggcaaaga atgaaggcgg ggattcctgg cattcggtca ataccatagc agagtctcta
      301 ggagtgacaa ctaaaactat aaacacctgg aataaaacct tagttgattt aggtatgatt
      361 gcacgtatag acgataacca tctcagtaaa tcgacaattt tacttcctct ctctagttac
      421 tataaattag cccttgatag ttctttaaag gatgttaccg agagcactgt ccatgaaatt
      481 gatggcaact tggttgcagt ttatcatttg tttgaatgga gaaaaaatga ggctgatgaa
      541 aattataacg ttccttataa cacactctgt tgtgttttta aaagaaaaac acaaaaagat
      601 actatctata aatttatctt ggtccaagat gagaaaataa gtaatttcga attaagcact
      661 ccttctagta agatttatga cgatgcatat agatttgaat gtcaagatct agaagccctc
      721 ctttccgcaa ataatctaaa caatatagaa catgaaaact ttgttgtaaa cactagattt
      781 aatttagttt ctgaaaagga ctcagatttg ttagatttgc tgatagagct tactaaaaat
      841 ctagataaga aagaaaacat tactgtacta taactagcat cggtccatca ttaagtttgg
      901 actattagaa cacttgctat tacagaaaaa ggtatgctag ccacgcttac tatttattgg
      961 tatcaaacaa gtatcttaat ttgaaaggaa gatgatttat ggctaattta tatatagaga
     1021 acaaaggaga cttgaatgcc attattactt taatcgtagt tatggtaaaa tcaataggta
     1081 atatcacaat aggaatgctg tttattatat tagcagcagt gctgattttt atgatagtta
     1141 tttggttctt aaatgaaaga tctaaagaca atcaacaccg tagggatatg gagataaaat
     1201 caatagagtc aatagattat ttatcgataa taacactaaa aaagccacgt tcctaaacgt
     1261 gacttacgct gaacgatatg tgttgtttgg tagacgaaca aatcgttcat tataaatata
     1321 tgatggtatc ctaatatagt aagcgtggct agcatacctt taattacttt accttttaca
     1381 gttaccatac acattttttg agcagaaatc aagtaaataa aaaaatatta tatttcacaa
     1441 catgctaata gactcatatc tagaacgcat taagtattta cctgtctggt tcgctgagac
     1501 ttctttttta actttcttca attttcttct ttattctaaa aagcgtactg cgactgaaac
     1561 cggttatagc ttcaacttcc ttatacgatt tacctgaagt aagcagttca tatgcgtgcc
     1621 tcttcttcgg tgtgattgtt gctttcggac gtccttctct gaaattagga ttattctttc
     1681 ttgcaaacat ttttccttct tgggtacgtt ccacaatcat atcacgttcc atttctgcta
     1741 cactcaacaa cgtccgtgtc accattcgcc ccatggacgt attttcaatc gttcctagat
     1801 tcaaaacctt gatcgttacg ttatcatcga gtaaaggttc aatgatctct aatgcttctc
     1861 tcgtgttacg agcaaatcga tcaagcttcg taacaactat ttcatctcca ggcttcagac
     1921 cagccaacag ctgattgaat tttggccgat cccgttttgt acccgtaaat ttttctgaaa
     1981 aaatattctt cttctctacg ccgttctcaa gcaactgttg aatttgttct tttgtacttt
     2041 gagaagcggt tgagacacgt gcatatccat atttcgtcat ttggtccacc tacttttaca
     2101 aatcatctat cgtatactca gaataccctt catagaaata gctgacatcg attcctttca
     2161 tatataactc gcgatcgtca attttatcag tcagagcatt ggaaattaga tagcgtattt
     2221 ccaaatcgtt tatgggactt cgctccattg cagaaagata gtctgcttta tcgattaagt
     2281 tccaatctac aactttttgc aattcttcct ttaaaatgag atccagccaa attctagtac
     2341 ttcggccatt tccttctcga aatgggtgag cgatattcat ctccacatat ttcttgataa
     2401 tctcttcaaa agaagtttgt ggcatctgat caatatgctg taacgaatgt tctaaataca
     2461 taactggtgc aaatcgaaaa tttccttttg caatattgac cgatcggatt tttccggcaa
     2521 aatcgtaaat atccgaaaat agaaattcat gaatttcaga taaaccttta aatgtaccaa
     2581 cttcgatttc atttattttt ccacaatcgt acaattcttt cgctttttgt ttactgagtt
     2641 tttcttcaac cttagccaac tcaacttgat tggttaatcc caatttattc tctaaaatca
     2701 tgcgaaatct ccttaacttt atgacataag tttatgaaac actctcaacc ccattatatc
     2761 gcgattttgt ttatgtgtca aaaactatta agtttctgac ccccgataga tttttcaagt
     2821 gtctctattt tgcacagtct ag
//
LOCUS       DAZKNH010000167         2739 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000167 DAZKNH010000000
VERSION     DAZKNH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.171"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="ACG8MG_002641"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="ACG8MG_002641"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0376629.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1072 a          425 c          558 g          684 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatata
//
LOCUS       DAZKNH010000168         2637 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000168 DAZKNH010000000
VERSION     DAZKNH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.173"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1452
                     /locus_tag="ACG8MG_002642"
     CDS             <1..1452
                     /locus_tag="ACG8MG_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0376630.1"
                     /translation="VEVTDNRSDADKYEPTVEGEKVEVGGTVDLTDNVTNLPALPEGT
                     TVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGE
                     KVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDV
                     STAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEG
                     IKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKP
                     QVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVT
                     ATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKT
                     GESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1586..>2637
                     /locus_tag="ACG8MG_002643"
     CDS             1586..>2637
                     /locus_tag="ACG8MG_002643"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0376631.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLL"
BASE COUNT         1034 a          370 c          531 g          702 t
ORIGIN      
        1 gtagaagtga cagacaatcg ttccgatgcc gataaatatg aaccaacagt agaaggcgaa
       61 aaagtagaag ttgggggcac agtagatcta acagataatg ttactaactt gccagcgtta
      121 ccagaaggaa caacagtaac agatgttact cccggcggta caattgatac caacacacca
      181 ggtaattacg aaggtgtcat tgaagtaacg tatccagacg gtacgaaaga tacggtgaaa
      241 gttccagtag aagtgacaga caatcgttct gacgccgata aatatgaacc aacagtagaa
      301 ggcgaaaaag tagaaattgg aggcacagta gatttaacag ataatgttac taacttgcca
      361 gcgttaccag aaggaacaac agtaacagat gttactcccg gcggtacaat tgataccaac
      421 acaccaggca attatgaagg tgtcattgaa gtaacgtatc cagacggtac gaaagatacg
      481 gtgaaagttc cagtagaagt gacagacaat cgttctaatg cagataaata caatcctgaa
      541 ggacaaaaag tgactacaga cttaaataaa gagccagatg catctgaggg aattaagaac
      601 aaagaagatt taccaaaagg aactacttat gcttggaaag agaaagtaga tgttagcaca
      661 gctggaaata aaaaaggtac tattgtagtg acatatccag atggatcatc tgatgaagtt
      721 gaagtagacg ttacagtgac agatactcgt tccgatgcag ataaatataa tcctgaagga
      781 caaaaagtga ctacagactt aaataaagag ccagatgcat ctgagggaat taagaacaaa
      841 gaagatctac caaaaggaac tacttatact tggaaagaaa aagtagatgt tagtacagct
      901 ggaaacaaaa aaggtactgt agtagtaaca tatccagatg gatctaaaga agaagtagaa
      961 gttactattt ctgtagtaga taaaaaagca ccgaataaac ctcaagttga tcctattaca
     1021 gagggtgacc aaattgttac tggtaaaact gaaccaaatg cagaggtgac agttacatta
     1081 ccagacggaa gtcaacacca tgggacagct gataaaaatg gtaattttac agttaaagtt
     1141 cctaaattag aggcagggac aaaagtgata gtaactgcaa ctgatgaatc tggtaatact
     1201 agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag acagtggaaa aaacggtagt
     1261 aaaggtagta agactgacaa tcaaggtagc aacagcaatc aagacaaaaa tagaggcaaa
     1321 agccagtcct caaaagtttt tcctaagacg ggagagagtg acagtaatat ctttactatt
     1381 agcggtggtt taatactgtt aggaacgtta ggattattag ggtataaaaa tcgaaaaaaa
     1441 gagaatgaat agaaaatcaa tttcttaagt cttctctgag aagaggaaga atctttttct
     1501 tcctcttttt gcttttttat gatgggaaat aagtgttagt taaagatgaa tatacttttt
     1561 atagaaagga aaagaatgta attaagtgga aaaaatagta attattggtt caaaccattc
     1621 agggctagcc gcagctcgat ttctaaactt aagcgattca aattatgaag ttattctaat
     1681 cgataaaaaa tcagaaatta gttacttgga atgtgggaca tcgttattta taaaaggaga
     1741 agtaagtaat ttagaagatc ttttttatca aaatagaatg acactaaatt ctgagataaa
     1801 aaagttattt ctagagacag aagttgacaa aattgatatt caaaataaaa agattagttg
     1861 tgtaaaaaaa gatggtacaa gattctctca agaatatgac aaagttatat tagctacagg
     1921 ttcaaagcaa attatgctag atataccggg gaataattta gctaatattt tttcgataaa
     1981 gaattttcca aatgcaaaag agatttttaa agctttggat gatagtacta ttaaaaaagt
     2041 aggaattatt ggagcaggat atattggagt tgaaattgct gaagccataa gaaaaagagg
     2101 gaaagaagta tatcttttcg atgttgcaga tagagtgttg tcgacttatt acgaccgtag
     2161 tttttcagat aaagttgaag aaatactatc aaaaaatgga atacatcttt gtctatcaga
     2221 aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa atagtcacta caagtggtac
     2281 ccaatactct atggatatgg tcgtacaagc tataggattt ctatctaata gtccaattgg
     2341 ggaaaaggaa ctcttgagag atgttagtgg agcatatcta actaatgaat accagcaaac
     2401 aaatataaaa gatatatatg ctattgggga ttgtgctaca acttttttta cgtccattaa
     2461 tagaagtagt gttgattttt ctatttctaa tgctctaaga acagcttata ttgcaactca
     2521 acatattaac aatcaagatt ttacaccttc tggttcccaa tttacaaatg gcttttctat
     2581 atttaattac aatttttacg cagcaggttt gaatttaaaa acagcacgat tattaag
//
LOCUS       DAZKNH010000169         2597 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000169 DAZKNH010000000
VERSION     DAZKNH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.174"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..1499
                     /locus_tag="ACG8MG_002644"
     CDS             60..1499
                     /locus_tag="ACG8MG_002644"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFQ0376632.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            1501..2463
                     /locus_tag="ACG8MG_002645"
     CDS             1501..2463
                     /locus_tag="ACG8MG_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0376633.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
BASE COUNT          928 a          406 c          461 g          802 t
ORIGIN      
        1 ttttcaacgg aaaacgttta ccatactagt tgtaaaaaga taaattgaag gagtaaaata
       61 tgaacttacc tcgagaaata cgatatcgtg cttattctga ttggactaaa gatgagatgg
      121 ataaaattaa ggataatgta aaacgatcac cttggcgtgc tagctatcat atcgaaccta
      181 aaacaggatt gttaaacgat cctaatggtt tttctttttt taacggaaaa tatacgcttt
      241 tttatcaaaa ttggccattt ggagctgctc atgggttgaa agaatgggta cacaatgaat
      301 cagatgattt agttcatttt catgtaacag gagcagaact tcttccagac acaaaacgag
      361 atagccacgg cgcttattcc ggttctgcat atgaaataga ggggaatcta ttcttactct
      421 atacaggaaa cgttcgaaat gaaaattggg aacgtgatac acttcaaatc ggagcatgga
      481 tggataaaga ctacaacatt acgaaaggtg agaatgttct tattcataaa cctgctgatg
      541 taactgatca ttttcgtgat ccacaaatgt ttaattatga aggacaattg tatgcgattg
      601 ttggagcaca aaatttagaa aaatcaggct ttatcaaact ttacaaagcg gatgacaata
      661 acgtggaaac atgggaggaa gttggcaacc ttgattttgg cggcactggt tcagaataca
      721 tgattgagtg tccaaatcta gtatttgtag acaaaaaacc tgttctcatt tattgtcctc
      781 aagggttgga taaatctgag ctaaattatg ggaatatcta tccgaatact tttaagatct
      841 gtcaatcgct tgacatagaa aaagtaaaac ttgtgggcgc ttcagaaatt caaaatcttg
      901 attatggatt tgaagcgtat gcgactcaag gattcaatac tcctgatggt cgtgctctga
      961 ttgtcagttg gattggacta cctgatatcg attatccaac tgataagtat gattatcagg
     1021 gagcaatgag tcttgtaaaa gaactttcaa taaaagatgg taaactttac caatatccag
     1081 tagaagctat tacatcatta cgtgctagtt ctgaacaatt tacagcaaaa gcagaaacaa
     1141 ataatactta tgagcttgaa ctaacttttc ctccaaatca gaaatcggag ttacttcttt
     1201 tctctgatag taaaggaaat ggacttagct tgattgtaga taccaaagaa ggaaaaatta
     1261 ttttagatcg cagcaaagca ggcattcaat atgccactga ctttggtacg attcgtgaat
     1321 gctcaattga tactaaagaa acaactgcta acatttttgt agataactca atcattgaga
     1381 tttttattaa taaaggagaa aaagtgttta ctagtcgtgt attccctgaa aatcaccaaa
     1441 atggtattca aatcaaatca ggcgaaccta ctggaaaata ctttgaatta aaatattaaa
     1501 atggtagcaa aattaactga tgtcgctgaa ttagctggag ttagcccaac aaccgtttca
     1561 cgcgtcatta ataataaggg atatttatca gaaaaaacaa aacaaaaagt tcatgaagct
     1621 atgaaaactc ttggttataa accaaataat ttagcacgtg gacttcaagg aaagtcacct
     1681 cagcttattg gtcttatttt tcctaatatt agtaatatct tttactctga actcattgag
     1741 tatctcgaaa tagagttatt taaacatgga tataaaacga ttatttgcaa tagtcaaaat
     1801 gatcctgcta aagagagaga atacattgaa atgcttgaag ccaaccaagt tgatggcata
     1861 atctcatcaa gccataatct tggaattgct gattatgagc gtgtgagagc acctattgtt
     1921 gcttttgata gaaatttagc tcctaatgtt tccattgttt cttcagataa ctttgaaggg
     1981 ggaaaacttg cagctaagac tttacaaaaa aatggttgtc aaaataccat tatgattaca
     2041 ggaaatgata acacggattc tcccacagaa ttacgtgcac taggtttttc ttttcaaaat
     2101 ccaaatggaa aaattttcaa aattcctaat aatctgtcta ctatccgtcg tgaaatggaa
     2161 ataaagtcaa ctattataaa tcacaaacca gatggtattt ttgtttctga tgatttaaca
     2221 gccattctca ctatgaaaat tgctcatcaa cttaaactta atatcccaga agatttaaaa
     2281 attattggat acgatggtac ctcttttatt gagaaatttt tcccacaatt gacaacaata
     2341 agacaaccaa ttgatgaaat tgcaagtctt attgttgata ttcttctgaa aaaaattaaa
     2401 ggcgaaaaga caaataagga ttatatttta ccaatctcaa tactttctgg aggaagtatt
     2461 taacgttaaa ttaatttttt aatgtctata aaattcgtta gaaaatacaa tttgtagata
     2521 tccaattgat ttgtaaacaa aaaggagttg tcaacttgta gtgaccccca aaagttagac
     2581 ttttttggag tggagaa
//
LOCUS       DAZKNH010000170         2542 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000170 DAZKNH010000000
VERSION     DAZKNH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.175"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1168
                     /locus_tag="ACG8MG_002646"
     CDS             14..1168
                     /locus_tag="ACG8MG_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFQ0376634.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1141..1584
                     /locus_tag="ACG8MG_002647"
     CDS             1141..1584
                     /locus_tag="ACG8MG_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376635.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
BASE COUNT          892 a          431 c          440 g          779 t
ORIGIN      
        1 ttaacacaaa ggcgtgatta ttttgaaacc caaaaagaaa atttatctag gtctcctctt
       61 ttccttgatg attggaattt ttagtatagg aattacaaat acctatgctt cagaaaattc
      121 taatttcaat atcaactccc aaccaattga agaacaagca attaattttg cgaaaactga
      181 ttttgaacac tccataactt caattctttt aatggaaaat aacaccgaaa ctgatattaa
      241 ctatactctt ggttcgccat ttaaaatgct cgatggagaa aatacggcaa atagttctat
      301 tttctttcca gtattttcag acgctgataa tacgattaaa tatgtttata ccgtcaaaaa
      361 aaatacaaat ggagaatatt caggaagtat ttcacaattt ttagcaaaag aacttcaaaa
      421 tctagtagat agtaatataa cagatacaac cttatttttt caagataata gcatttatta
      481 ttccgataat ggacaaatta aactactttg gaaatcacct ttaagtacag aaaaaaatgg
      541 tgtttcttcc cttacagaaa aaaatgatct tatatccgta aatataaaaa ataaaattaa
      601 aaatttttct aaaaaacaaa attttcctgc ggttaaacgt gtaaaaagtg tctcctccgg
      661 agataacttt attatgatta attggcgtat agcagaaacc caaacaagtt ttccgtggtg
      721 cgctgcttac gtagaagctg ctatcttatg taacaaaaca gatgtacgtg ctacttcagc
      781 tagagccatt atgcattata cctatcctaa tttaaacaac agtcaaattg aatcaaaaag
      841 tcttaatcaa gatcaaataa taaaatatgc aaattactgt ggtgtttatc ctaaaaaaat
      901 agcacgaata ctaaaccttt cagaggtcca aacacaacta agaaaaggaa atgccattta
      961 tatgggaact gctggttatg gaaattatag taacagccgt catgcacttg cattatttgg
     1021 gtgggtcaat caaggtggtt atcaaactta ctatgtgtgg aatccttggt ataactaccc
     1081 aactgtaata agtgcaaatg caggaccagt tacaattcct gtacctggag gtggttttac
     1141 ttgggacaat accattacaa attggtaaat aaactcattg ctttagttat gggtctagtg
     1201 ttagttcttt tcttaactag ttgttcaaaa aaacaagaaa tacaaaagac tgatctgatt
     1261 ccttttctag gaacttggca aactaaagat cattcagaac gtctagtaat cactaattta
     1321 aatacttcaa aaaatataga acaatatacc atcttagtaa aagagaacaa tcataaaatt
     1381 caaatcattg ataaaaattt aaatataaat gaacaagttt tcatttacaa agcaacaaat
     1441 aatttactaa cactgcgctc gaataaaaaa caattagatt acacttttag tttggattct
     1501 ccaaaaaagc tcgtatttat tttgacaacg aatgatggga cagaagggac agctaaacca
     1561 attgaatttt ttaaagctga ttgatgaata gttgtttgat attacaaaac aataatttca
     1621 ccgaaggtga aacaagcgaa actttacaaa aagtggctag catttgctag ccttttttgt
     1681 attgtggagc gtgggttttg agggggattc aagggggagc ttgcgcccct ttgacaagcg
     1741 gttatttgca ccaaatacgt aggtatttgg ctagcaaata tagagcttgc caagcccttt
     1801 agtgtgtttt gtagaaaggg accttatagt cactttcaca ttacaaacga tacgtcctat
     1861 ggacgtatca aaagggtggg acttatgagc aaattaaagc aaaatttggc tagcaatcct
     1921 tctaaaaaat acacctattg atcagagtca aaataaatca gttttcgagt gagcgaatcc
     1981 aaatttgcaa ctctgaagct ttgcaaatga gtgtgccggc ttccgtaaaa gtctaggtaa
     2041 cattagtcga tatatacata gggctctaat ttgcgtttta aggcgtttta aacgttctgg
     2101 tatgtaatta taccaaagcg actttaaaac gccttaaaac gaattttaga ggttctgtag
     2161 aacgttttag ggttgttagg gcttgccctg accgcctgta aggcgcttta aaaacaagat
     2221 aagggatttt atctagactt gtcttaaaac gccgtagatg agcgctcaga gcttctaaaa
     2281 ggactcctta cgaactgttt ttaagcaaag gagcctttag gcgactgcgc agtttaatgt
     2341 gagagcgtag cgatcacacc tttaaagcga ccgtagggag cagattccca gtttaggaaa
     2401 tttcgcgaac ctgtttggag aaaaaaagta ggaaaatttt aggcgggaaa ttttgccccc
     2461 ctcttttata atctctttta taacctcttt ttaaacctct ttaccgtcgg ctgtatcctt
     2521 actctcccaa ggtgtgcaga at
//
LOCUS       DAZKNH010000171         2540 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000171 DAZKNH010000000
VERSION     DAZKNH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.176"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..2034
                     /locus_tag="ACG8MG_002648"
     CDS             88..2034
                     /locus_tag="ACG8MG_002648"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFQ0376636.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            2298..>2540
                     /locus_tag="ACG8MG_002649"
     CDS             2298..>2540
                     /locus_tag="ACG8MG_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0376637.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT          844 a          412 c          448 g          836 t
ORIGIN      
        1 acgtttgaca tatttttaag atatgctaaa ataaatttgc atttcagtga accgttttca
       61 cgtaaaaata aaaaaggagt gattcgaatg aattacagta aagtagcttc cgacgtaata
      121 caagcggtcg gaaaagataa ccttattgca gctgcacact gcgctacacg tcttcgtctc
      181 gtactaaaag atgacacgaa agtgaatcaa aaagctttag acgaaaatcc agatgtgaaa
      241 ggcacattta aaattgatgg acaatatcaa gtgattatcg gtgcaggtga tgttaatttc
      301 gtttatgacg aacttatcaa aaaaactgga ctttcggaaa tttcaacaga tgatttaaaa
      361 caaattgcta gtaaagataa aaaattcaac cccattatgg cattaattaa attattatca
      421 gatatttttg ttccaattat acctgccctt gttgcaggtg gtttattaat ggcattacaa
      481 aactttttaa cagctgctgg actttttgga ccaaaatcaa ttgaagaaat gtatcctgcg
      541 attgaaggta tttcctcaat gatccaactg atgtccgctg caccattcat gttcttacca
      601 attttagtag gtatttcagc tgctaaacgt tttggagcaa accaatttct tggtgctgca
      661 attggtatga tcatgacaac accagatctt ggtggctcaa ctgaatattg gaatattttt
      721 ggttatcatg tttcacaaac aaactatgct tatcaagtta ttccagtttt agcagctgtt
      781 tggatattat ctatattaga aaaattcttt cataaaaaat tgccatcatc tattgacttt
      841 acattcacgc cattattatc agtaatgatt actggatttt tgactttcac agttattggc
      901 cctgtaatgt tattgttgtc taatggaatt acagacatta ttgtttggct atataacact
      961 actggattta ttggaatggg tatttttgga ggaacatatt cacttatagt catgactggt
     1021 ttacatcaat catttccagc tattgaaact caattacttt cagcgtggac aaatggtatc
     1081 ggacatggag actttatctt tgtagttgca tctatggcaa acgttgctca aggtgctgca
     1141 acatttgcca tttggttctt aactaaaaat tctaagacaa aaagtcttgc ttcctctgcg
     1201 ggtgtctcag cacttcttgg aattacagag cctgccttgt tcggagttaa cttaaaatac
     1261 cgctttccat tcttttgtgc tttaattggt tcaggaattg ctacagcagt agcaggatta
     1321 cttaaagtag ttgctgtttc acttggttca gcaggtttct tgggatttct ttcaattaat
     1381 gcaacatcta ttccatttta cgtattgtgt gaattgatta gctttgtagt tgcttttgca
     1441 attacttact tctacggtaa aacaaaagct gctggtatct ttaaagcaga agaaattgct
     1501 gaacaaacac cagttaattc ttcaaaagta gcaacaacag atccaatatc tactgatgtt
     1561 gaatcaacac aaacaaaagt ttcatcagaa acaattgtta gtcccatttc tggtaaagtg
     1621 attagtcttt cttcagtaaa tgatccagtc ttttcatcag aagctatggg aaaagggatt
     1681 gccattaaac caaatggaaa cacggtccat tctccagtag atggtactat tcaactcacg
     1741 tttgaaactg gacatgctta tggtattaaa tcaaataatg gtgctgaagt tctaattcat
     1801 attggtattg atactgtatc aatgggagga aaaggattta cacaaaaagt tactactaat
     1861 caaaaagtta gaaaaggcga tattcttgga gcatttgacc gttcaatgat tgagaaagaa
     1921 ggtcttgatg atacaacaat gattattgtg accaatactt cggattattc agaagtaaat
     1981 atagtagctg aaaatacagt cactgagggt actgatttac ttgaaatcaa ataaatatat
     2041 ttccctgagt aaagatggaa ttttctttcc aaaatcaaat aatatagatt aatcaaatat
     2101 ttgagaataa cattttattt aatcttttca tatatcaatt gtatgaaaaa ttattagaat
     2161 tttaaaaaat gataaaaatt caaaaagaaa aaatagtagt tatttacttg tcatgttact
     2221 aaataaaaaa ataatttaaa tatgatatat agtcatttat ttagtgattt cattctaaaa
     2281 taaagggaga aatgtctatg ctatacggtg caattgaagc aggcggcaca aaatttgtgt
     2341 gtgccattgg cgatgaagaa atgacgatca aggaaagagt gagctttccg acaacgacac
     2401 ctgaggaaac aatgccttta gtcattgact tcttcaaaca ataccaagca gatttagcag
     2461 ggattgggat tggctcattt gggcctatcg atatccatcg cgattccgcc acttatgggt
     2521 acattacctc gaccccgaaa
//
LOCUS       DAZKNH010000172         2530 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000172 DAZKNH010000000
VERSION     DAZKNH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.177"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(145..1083)
                     /locus_tag="ACG8MG_002650"
     CDS             complement(145..1083)
                     /locus_tag="ACG8MG_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0376638.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            1574..2530
                     /locus_tag="ACG8MG_002651"
                     /pseudo
     CDS             1574..2530
                     /locus_tag="ACG8MG_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT          874 a          437 c          399 g          820 t
ORIGIN      
        1 aatattaaaa gtcatatata acttcctcct aaattttaaa tttttatatt taggaggaat
       61 aatcctctga ttttttcata cgttatgtca cctcgtaaat attaattata ctgaattagc
      121 aatttttatc aaataaaact tattttactt ccaaaaccta aattcacgtt gccaaaaatc
      181 aatctgcttt tgcaattgtt tttcgttcgc ttttaaagtc gatttcatta attccgttaa
      241 atcaattgga gatatttctc taatcaattt tttaaattta gtcttagtat tcttacttag
      301 ctttccccac atactttctt catgcaacaa agtataaacc atagcttgct cattaatttt
      361 ttctaaagta gcccacgcag gtttcaagat gtgtaaatca ttaaaacaat cattccagta
      421 atcaaccata tctcttttta attcaacttc tacacgccat aaatgttcag acacaacttc
      481 aacatctgcg ttatctttac gttcttgttt tttattataa attctaataa atctatcact
      541 atcacggaca ccaaaatatt ttgtttctgg cttgccatta cgaccataaa aaacagtttt
      601 cttaactgct ttatcagtca ttgcatagta atcgctcaaa tcatcttcaa aatcaaaagc
      661 taagtctaat cttgtaaaac cgtcatcttc catgtagtcg ataatatttt gttttaacca
      721 aatcatttct tcatgtgtga gtttattggg attaaattca acacgcatat tacgtctatc
      781 ccaagtatct gcttttactt tgtcatattc gatataaact ttttcttgaa gtgccttagc
      841 tttaaacttt gtttgaagta tatcccaaag tcgtatttgt ggctctacac tcataaagtc
      901 agatagcttt ttagcattag ttttgttcaa atttccaacg attgtcatgg catcaaaact
      961 taatgcgggt tgagattttc ccaaagtttg accacttaac cggctattac ttaaccggct
     1021 attagagacg gccggttttt ttagtgagat ttctgcagct aatttttcat caattttctt
     1081 cattttttat cgctcctaaa ataatttttg gaacgaaaaa aggacgagtg gaatcgcaaa
     1141 ataaatttcg cctgtatcca ttcgtccatt ttgttttgta ttcaaaatca aaaattgata
     1201 tactaatcat cgaatgaata acaagtgtgg tttgttattc cgggcgagaa cgttggtagc
     1261 gttgctcgtc tttttttatg tctttttttc gttaaaacta ttttaaaact aaatatatct
     1321 taaagcaaat aaattcaagg ggctaaaagc gccccttaaa aaccccttgc ctctgaaaag
     1381 ttttgtgttt cctttccgtc ggtttcaaaa gctaatttac tcaaaaaaat agatcaaggg
     1441 taaatgcact cactgcgttc gcccttgagc tattttattt cgcaaatttt aacgcatttg
     1501 ccgacgaaaa gataaaacac ataaaaatat acgcagatat atttaaaacg tcttagaacg
     1561 caaaatttta cgtttaaaag ctatgcaagg actgtatgaa gcaaaattat tgagttatcc
     1621 aagaacagat acaccattta ttacagagaa cgaatttgct tatttaaaag cgaattttgg
     1681 caaatatagc ggttttttag gacttgatct tgaaatggtt caaacagagc ctagaaagcg
     1741 ttatgtggac ggtagtaagg tacaggaaca ccacgccatt atcccaacaa aacaagtacc
     1801 taccgaatct gcattagcga aaatggacga tttacaacga aaaatttatg ctttagtcgt
     1861 taaaacgacc gttgccatgt ttttacctga ttacttgtat gaagaaacca agatacaaac
     1921 gaaagtagct gatttgctgt ttcaatcgat cggaaagaca ccaaagcaag aagggtggaa
     1981 aattcttttc aaacaacaaa ccaaagaaga aaaagaggac gttcaaacgt taccactcgt
     2041 tatcattggc gaacgtgccg aagttggtgt taagagtgtt gaaaaagaaa cgcaaccgcc
     2101 aaaagctttt acagagggta cattattaac tgctatgaaa acggcgaata aaacggttga
     2161 tgatgaagaa gcaatcaaga ttttacaaga agttgagggg attggaacag aagcgacaag
     2221 agcaagtatt attgaagcgt taaaacaaaa agaatatatc caagtgatta agaataagct
     2281 tgttgtaact gaaaaaggaa aattattgtg ccaggcagtt gaaagtcagc accttttaac
     2341 gagtgctgaa atgacggcta aatgggaaac gtatttaaaa aaaatcggta aaagagaagg
     2401 caatcaagag aactttatta cgaatatcaa aaaattcatt gttcatttac tggaagctgt
     2461 acctaacgat atagaaaaac taaatttttc tgattaccag gaacagaaag aaaaagaagc
     2521 agaaaaaagt
//
LOCUS       DAZKNH010000173         2459 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000173 DAZKNH010000000
VERSION     DAZKNH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.178"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..1150
                     /locus_tag="ACG8MG_002652"
                     /pseudo
     CDS             6..1150
                     /locus_tag="ACG8MG_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1321..1779
                     /locus_tag="ACG8MG_002653"
     CDS             1321..1779
                     /locus_tag="ACG8MG_002653"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFQ0376639.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="ACG8MG_002654"
     CDS             1938..2408
                     /locus_tag="ACG8MG_002654"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFQ0376640.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          823 a          460 c          460 g          716 t
ORIGIN      
        1 gaaacatgca cattcaaaca aaattgatcc acggaggcat cagtgaagat cccacaacag
       61 gagctgtcag tgtacctatt tatcaaacat ccacttatcg gcaagatggc gtcggccagc
      121 ctaaacaata tgaatattca agatctggta atcctactcg ttttgcattg gaagaactga
      181 ttgctgattt ggaaggcggt gttcgaggat tcgctttttc ttctggtctt tctgggattc
      241 atgccgtttt ttctcttttt caagctggcg atcatatctt attaggagat gatgtctatg
      301 gaggaacatt ccgtctattt gacaaagttc ttactaaaaa tggtctcgag tacacgatca
      361 tagatacgag taatcttgat aaaatagaac aatccatcaa acccaataca aaagcacttt
      421 atcttgaaac acctagcaat ccgcttctaa agattactga tttagaaaaa agtgccactc
      481 tggctcatca acatggattg atagttattg ctgacaatac atttgctact ccttattttc
      541 aaagaccttt agatctcggt tctgatatcg ttgtccatag cggtaccaaa tatctaggcg
      601 gacacagcga tgtagtagct ggtttagtga cttctaacca aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgcctt
//
LOCUS       DAZKNH010000174         2405 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000174 DAZKNH010000000
VERSION     DAZKNH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.179"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..103)
                     /locus_tag="ACG8MG_002655"
     CDS             complement(<1..103)
                     /locus_tag="ACG8MG_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0376641.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
     gene            complement(135..458)
                     /locus_tag="ACG8MG_002656"
     CDS             complement(135..458)
                     /locus_tag="ACG8MG_002656"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0376642.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTHFGSVAKFSK
                     KSILV"
     gene            920..2302
                     /locus_tag="ACG8MG_002657"
                     /pseudo
     CDS             920..2302
                     /locus_tag="ACG8MG_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          875 a          412 c          400 g          718 t
ORIGIN      
        1 acaattcctg aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa
       61 taatgacgtc ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt
      121 cttttttctc aattttacac taaaatagat tttttggaaa actttgcaac agaaccgaaa
      181 tgagtatagt atgaattagt aatttggttt tcatctaagt ttagctttga tttactaaaa
      241 ttgatatctc gatacatgtt caatacatct aatacaaatc gagaatcttc tttcggaaat
      301 ggctcagaaa gatcactcca aatttcatca tcataatgat actcgtaacc ttctctcaat
      361 atttcgattt ttctttcata ataagatctg ttttggtcct caaatttagc caataactca
      421 aattgattta ctagtgacaa tctctgaaaa ctgtccatta aatgtttctt cctatcttgt
      481 tcattaacaa aaaattataa gcccttacaa cgaatattat atattaaatt taattgtagg
      541 tgaacacgaa ttttataaat atttatggtg ttaatgacaa atgatatccg actcctcttc
      601 atacgttctg gactatttgc taatagatta aaatttcagc atctatttct gaccataaat
      661 aaatgagctg ccgaaaagct gccaaatcag acttgaaaat aaaggatcaa aaacttttaa
      721 gtttctgacc ccgtaactca cacttcaaaa tcttacttaa aaaggagtct gaaagaaaaa
      781 aagagtattc aaaatttttt tctaactagc tacatcacat ctatgcattg acactcaaag
      841 aaccagaata agtcatcaga aagcaagggg gtgcggacat aatcctggac gaaaaaataa
      901 acttaggagg aatgtccgtg tgttttccca caaagagcgt gtaagggcaa ttcagttatt
      961 tttaaaatac gattgctctt atgctgcaac cattcgagaa ttagggtatc cgtcgattgg
     1021 tgcactacgg aaatggtata aagagtattt ggaatccgga gagctccatc aggagcaccg
     1081 aaaaaaatca aagtattctg aagaacaaaa acgaatagcg gtgaatcatt attttgaata
     1141 tggtcaatgt tatgcacgaa ccattcgcat gcttggttat cctaatagag agtccttgcg
     1201 gcagcggtgt gaagaacttg caccaggagc tagaaaactc cgaaaaagtg cggtaaagtt
     1261 aacacaagat caaaaagaag ctgtgttgaa aaaattctat aaaccgcaaa ctaataggaa
     1321 aaatttagcc gaatctggag gaattagtag agttactcta tatcaatgga aaaacacggt
     1381 tcttggaaag gattttccat tgtgtatgac tttaaaaaat caggaaaaac aaaaagagct
     1441 acttttaaag gaagtagaag acttacaaaa acaagtacac caattacagc tcgaaaaagc
     1501 attactcgaa ggagcggctg aactgctaaa aaaagaaaaa ggcgtcaatc ttctttgtct
     1561 atcaaatcaa gaaaaaacga tattgattga tgccttacga aatcagttca ccttgaaaga
     1621 gcttcttcaa cagctccagt ttcctaaaag cagctatttt tatcaaaaac aagcacttga
     1681 gaaaccagat aagtattaca aagaacgtca aattaattat cacgattttc aatcataact
     1741 tctgtgcgta tggttaccgt agaattcacc aagctttaaa aaatatgggt aaaaagcttt
     1801 ctgaaaaagt tgtacgtaga ttgatgactg aggaaaatct atttgttaaa ttttctagac
     1861 gaaaaaagta ttcttcgtat gctggggaaa tttctcctgc acaccccaat ttattaaatc
     1921 ggaattttaa agcaaagaag ccaaatgaaa aatggttaac agatattact gaatttagaa
     1981 ttctcgcagg gaaaatctac ttatcaccac ttgtcgattg ttacgatggt gctattgtta
     2041 gttggactat tggaacaaag ccaacttgct tcactctatg tctaaaaaga gttattcacc
     2101 agataattca gcgtgcgaag gtttctttgg acgattaaaa aacgagatgt tttatcaacg
     2161 tgactggaag aatacaacaa tcaatcagtt tataaacaaa ttggatgatt atattcattg
     2221 gtataataat caacgaatta agttatcttt aggaaggcta agccctcttc aatatcggaa
     2281 gaagcaaaga tatatttgtt aaaaaggtcc aaaaaaaata tccgcacccc ctttttttgt
     2341 aaaaaactat tgcacaatac aagtagccta cggctatcga aatttccata actttcgagc
     2401 aagaa
//
LOCUS       DAZKNH010000175         2398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000175 DAZKNH010000000
VERSION     DAZKNH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.180"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACG8MG_002658"
     CDS             207..2069
                     /locus_tag="ACG8MG_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0376643.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="ACG8MG_002659"
     CDS             2069..>2398
                     /locus_tag="ACG8MG_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0376644.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAZKNH010000176         2395 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000176 DAZKNH010000000
VERSION     DAZKNH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.181"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACG8MG_002660"
     CDS             316..570
                     /locus_tag="ACG8MG_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0376645.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACG8MG_002661"
     CDS             571..888
                     /locus_tag="ACG8MG_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0376646.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACG8MG_002662"
     CDS             1447..2382
                     /locus_tag="ACG8MG_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376647.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAZKNH010000177         2387 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000177 DAZKNH010000000
VERSION     DAZKNH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.182"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(103..1650)
                     /locus_tag="ACG8MG_002663"
     CDS             complement(103..1650)
                     /locus_tag="ACG8MG_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFQ0376648.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1752..2105)
                     /gene="tnpB"
                     /locus_tag="ACG8MG_002664"
     CDS             complement(1752..2105)
                     /gene="tnpB"
                     /locus_tag="ACG8MG_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFQ0376649.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2095..2289)
                     /locus_tag="ACG8MG_002665"
     CDS             complement(2095..2289)
                     /locus_tag="ACG8MG_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376650.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          670 a          492 c          431 g          794 t
ORIGIN      
        1 tttttatttg gtcttgaaga agaaaagtaa gcgccccata gaacagtacc tgtacgtcaa
       61 ataacccgac ctatagaata gaatcgggaa tgtttggcgt ttttagttac gagaaaaatg
      121 ttgttgtaca tccttgttcc aaggcaacag ttgatctaaa gtttctgctg aaagtacggg
      181 aagatttggt atcttatcaa atagaaactc tagatacttt acaggattca atccgttaag
      241 ttctgctgtt ttatatacac tcagaatgat tcctgaagtt cgtgctcctt caagacttgt
      301 ggaatgcaac caatttttcc gtcctatcac cagctctttg atatttcgct ccgcacgatt
      361 atttgtgagt tctaaacgac catcttcaaa aataggttca aaaccacttc gtagtttaca
      421 cgcatattga acggctgcat ccaacttgga ttttgcaaca ggatctatcg ttgccatcca
      481 ctcataaaaa gcaataaata atggacgcag ttcttgttgc cgacagctta atcgcttctc
      541 aggagaaaag tctttccatt tcttttctag gacaaaccat ttatccaatt gtttgactgc
      601 ttgagctgcg ggaatatccg tgtttttctt tccttttgga attgcttcga aaaatttccg
      661 ccgaacatgc gcgaaacaat agacaagtcg tacactttcc acggcattat acccgctata
      721 gccgtcacag tgtaaatacc ctgtaaactc cttgagaaaa gttttcggaa catctccagc
      781 acgactttcg tcgtggtgat aataaacgat gggatgctga ctgtcttttc ctgtacaaaa
      841 ctgccagtaa taggtatctg ttttggcact ttcaatcaca cgataacttg tttcatcccc
      901 gtgtagaatc tcttcttgat taagatagac tcttaatcgt tctcctaaag ggtctaacgc
      961 gttttgcact gctttaatat gccaattggt gatattatct ctcgaaatat ccaacccatg
     1021 tgtttgtttc caatagttct cttgccgata ggcaggaact ttctgaccaa atttcatccg
     1081 gatcgtttcc gccactaaac tattggaacc taagctatta gaaatcaaag gtttaggaac
     1141 gatcgctttt ttaaaagaag aaagtccttc tttttcgcag cttttacaac aataggtatg
     1201 ttgataatgg gtgagacaat ccaacatagc ctgatggaaa cgaatttctt ctcgtacttt
     1261 tgtccgtccg atatctttca tctgtgaacc gcaatgttca caaaattgtt cttccaaagg
     1321 aaggagatgt tctttctctg ttataggtaa gtgtttgatt ttgtctgctt tcgttcctac
     1381 aactcgttga tgtcctttta ctggaataat ggtggcagct gcttctggaa cacttacaga
     1441 aatttcttca tcactaaaaa gattcaattg cccattcgtt tcctcaaaga gagtctcttt
     1501 agagcgtcca aataatttgt tggttaaata attgaccttt tcattcaata gttggatttc
     1561 agttgtaaga ttctgaatgg tttgttgttg tgcttgattg gttttatggg cttcttgaag
     1621 caactgaagc aacgtatctg tagtttccaa aatttttcac caccttgaat cgaactgatt
     1681 ttgttacaga ataccacaag aaacgcacaa gaaatagggg ttcaacacat tttttgtgtt
     1741 aaccccccag cttaaaaaac tccttttttt cctggcaaaa tagctcgagg ttgatcgata
     1801 cttaatcctt ctaacaacca tcgaacctgt tggttcgtta aggttcgaac ttcgtctttc
     1861 gtccgaggcc attttaactt gccgttttct atccgcttat aacagagaag aaaaccatca
     1921 ccgtcccaat agagaagttt gaaacggtct tgacggttcc cgcaaaataa aaaaacggca
     1981 tcttcgtaaa gctcgagttg atactcttgt tgaataagac tggcaagacc atcaattcct
     2041 tttcgcaaat ctgtttttcc gcacacaata taaatatgtt tggtttgaag taaatcataa
     2101 agcattggac aacagttctt tcaaaatagc ttgaagaata taattgttaa taccgttata
     2161 gatcaaaact tcagaaccat tctctctttt gatgcgcatc gctaacttgg cagccgcggg
     2221 tttacctttt tctggtgcac tcttttcgtt tcctaattgg acaggaacaa tctcatttaa
     2281 tttatacata gaagacctcc agacttcttg atagctcaag tatatcttga ggtgcatttg
     2341 ttagtaaggt actgttctat ggggcgctta cgttaaaaat gttttgc
//
LOCUS       DAZKNH010000178         2255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000178 DAZKNH010000000
VERSION     DAZKNH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.183"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG8MG_002666"
     CDS             complement(<1..914)
                     /locus_tag="ACG8MG_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFQ0376651.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG8MG_002667"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG8MG_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFQ0376652.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="ACG8MG_002668"
     CDS             2006..2176
                     /locus_tag="ACG8MG_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376653.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT          759 a          390 c          427 g          679 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attct
//
LOCUS       DAZKNH010000179         2107 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000179 DAZKNH010000000
VERSION     DAZKNH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.184"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..1024
                     /gene="msrA"
                     /locus_tag="ACG8MG_002669"
     CDS             191..1024
                     /gene="msrA"
                     /locus_tag="ACG8MG_002669"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0376654.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1104..2093
                     /locus_tag="ACG8MG_002670"
     CDS             1104..2093
                     /locus_tag="ACG8MG_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0376655.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT          762 a          351 c          424 g          570 t
ORIGIN      
        1 tttccataaa aggtaaatta acttttcatt tataaaataa attgattaaa aaggagaatc
       61 tgtcgataag gattctctct tttttatacg aaaaatctgt agggggatga agtaatcgct
      121 tatttcctca ctttatgata aattacagct aaagtcacta gcttgtcttc ttcggaaagg
      181 aggagaatct atggatgagg aacaaatatt cattcaaaca ctttataatc tgattttgaa
      241 tccgaataca cgagattggg aacgaaaagt attgattcag acaaagaatg acataagaga
      301 aaatatatcc gttaaagaac agttatcaaa actagaagca acactacgtc cattagctat
      361 ccgaatgaat ctgacgcctg atgtaatgga cttttattta ttgattacgg aaggatttga
      421 taaggagcaa aaatatgact tttccaagca tgccatgcaa gatgctgact atcaagaacg
      481 tgcagtattt gcagggggat gtttctggtg tatggtcgag ccgtttgaat cgaaaaaagg
      541 gatcctatct gttttgtcag gatacacggg cggacatgtt gaaaaaccaa attatgatca
      601 agtaagtggt ggttacacag gtcatgtgga agctgtggag atcatttatg acacaagaga
      661 aataagttat agcgagttgc tgacaatcta ttggcaaatt actgatccca cagatacatt
      721 tggacaattt caagatagag ggaagcaata tcgtccagtg attttttatc aagtcgaacg
      781 acaaaaagaa ttagcggaac aatcaaaaca gaaactagac agttcaggaa ctttccacca
      841 accaatcgtc acaaaaatcg aaccagccgg tacgttttgg ccagcagaaa actatcatca
      901 gcaattttac aaaaaacaac cgaaacgtta taaaaaaatc cagcaagcac gtaatcaatt
      961 tctgatctat caacgagtaa aaaacaaatg gcaaaaaaat atacgaaaaa atcattttga
     1021 ttaactcctt ttctttaaaa tgtagtaaac taaaaataga aaaaagaaag cgttttaaaa
     1081 agatttgatg aggaggagaa aaattgtata ttgcagatag tgcacgttat gaaaaaatgg
     1141 aatatcgtaa atccggtcat tcaggtttga aattaccgat tttatctctc ggattatggc
     1201 aaaattttgg tgattatgat cctatccata atcaaagaga gattttaaga ggcgcattcg
     1261 acatgggaat tacccatttc gaccttgcga ataattatgg aggccctgct ggagctgcag
     1321 agaaaaactt tgggcgtatt tttagagaag attttcaagc ctaccgtgat gaactgatta
     1381 tttctagcaa agcaggttat catatgtggg aagggccata tggggaatgg ggctctcgta
     1441 aatccatcat ttcaagttgt gaccaaagtc tccaaagaat ggggctagat tatgttgaca
     1501 tcttctatca tcatcgccca gaccctgata cacctttgga agaaacagca gaagctttga
     1561 tgcaacttgt aagacaagga aaagcattat atattggtat atccaactat aatggagaag
     1621 atacaaaaaa aatgactgaa attttaaaaa gaaaggaagc accgttcatt attcatcaga
     1681 tgcgctacaa tatgttttct cgtgcactac tagaagacga tctatctcca gtattagaag
     1741 aagaagggct aggggcaatc acgttcagtc cgcttgctca agggttgctg acaaatcgtt
     1801 atctacatgg tattcctgaa gattctagag cacatcgtaa ggaaatacca ttcctttcag
     1861 aagaacaagt tggatcaact ctggaaaaaa tcaaagcact gcaaacaatc gccgtatcac
     1921 gaggacaatc attagcgcaa atggcccttg catggaattt gcgacaaaaa tctgttacaa
     1981 gtgtactggt cggagcaagt cggttaagtc agttacaaga aagtgttcga atgatggata
     2041 atttagattt ctcaccagaa gaagaattga gaatcgacca aattctagaa tagaaacgat
     2101 gtaatca
//
LOCUS       DAZKNH010000180         2002 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000180 DAZKNH010000000
VERSION     DAZKNH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.185"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..377
                     /locus_tag="ACG8MG_002671"
     CDS             <1..377
                     /locus_tag="ACG8MG_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0376656.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            425..1825
                     /locus_tag="ACG8MG_002672"
     CDS             425..1825
                     /locus_tag="ACG8MG_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0376657.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          554 a          339 c          432 g          677 t
ORIGIN      
        1 aaaatgtttc cacagaggac ttctttaatt attgtcgatt agaggaaagt ggtgtaacag
       61 tcactagcgg tacagattta ccgctgttta ttactgatat tcctgaatcg attataagag
      121 cagtattccg tcgttttcct gaaggagaag aagtctggga gaaagaaaca agcttcacga
      181 caaccgagct gctgaaaagt tttacaagca acagcttcaa agtaaatggt ttatcggagt
      241 acggagaaat aaaagtaggg aaaagcgcca gcttagctgt tttcagcgag gacctctgta
      301 ctgatgactg gcaaaaagtg atggctgcgg aagttatcgc tacctatctt gatggtgact
      361 tagtttacaa gaaataatgc ttttcgatat aacgttataa aacgaattaa agagaggtta
      421 aaaaatggaa acaatgaaaa gaaaattcac cttcccacat agttatacat tgatttttat
      481 cttaattatt ttggctgcaa ttttaacttg gataattcct agtggtcagt ttgagacagt
      541 tgttgaaaac gtcgcaggta tggaaaaaac aacggttgtt ccgggaacct atcaccaaat
      601 cgataaggca gattatggac aaggtttttc ctctatacta atggcaccag ctgaagggat
      661 tatttcagct gttgaggttg ttgcttttgt tctcatcgtt ggtggtgcct tcgggattat
      721 cctaaaaacg ggagccatcg atcgagggct gtttacctta gcaaaagttc tgggagacaa
      781 ggggatctta gttattccga tgtcgatgat tctattcagc ttaggaggat caacctttgg
      841 catgagcgag gaagtcattc ccttatttgc ggtgtttatt tctctgatgt tttctcttgg
      901 ctatgattcc atgactgcga tcttgattct atttttaagt acacaagcgg gatatgttgg
      961 gtcgacgatt aatcccttca acgttttaat cgcacaggga gtagctggcg tttacggaaa
     1021 tcccctgctt tggtttcgga tgctatcctg gattgtcatt actgggattt caattgtttt
     1081 caccatgctt tatgctagca gagtcaaaaa gaatccactg tcctctattg tttatgagag
     1141 tgatttaaag gttaagcata agtttcaaaa ttttgacact gaaaaagagt tttcaacaca
     1201 agataaacta attatcgccg gttttatcgt aggtttagga atcatgattt ggggtatcat
     1261 ttccaaggga tggtatatgg ttgaaatttc tgcactattc ttaggattag gtctttttgc
     1321 tgggggcatc gctagatttt ctcaaaagga aattgctgaa gcttttgtcg aaggctgttc
     1381 agatttcgcc tatgctgctg taatcattgg gctagctaga ggagtcttgg taatcttgga
     1441 aggtggaatg attattgaca ctattctaaa cagcttggct aaccttttag gtggtcttcc
     1501 aaaattcctg ttcactacga ttctactcat ttctcaagta attgtttcct tttttgttcc
     1561 atcctcctca ggagctgcgg cattaacgat gccggttatg gcaccattgg gtgatctaat
     1621 gaatgtaagt cgtgatgttg ttgttctaag taaccagttt ggaaatggtt taatgaatat
     1681 tatctcccca actagtggtg tattactggc aggattagcc atcgcagaga ttaatttctc
     1741 taaatggttt aaggttggct tcaaaatatt tgccatctta acccttgttt ctgctgtttt
     1801 gttatacatt gctacactct tttagtctta atcatccatt aataggaggc aatagttatg
     1861 attaaaataa atcaattagc aggaatgaat atccactatt tattttattc tctcaaagaa
     1921 tttttcgctt atcaacagaa aattggtatt tcttctgttg aactttgggg aggtgctccg
     1981 cattttttca tagatgcttt ta
//
LOCUS       DAZKNH010000181         1981 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000181 DAZKNH010000000
VERSION     DAZKNH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.186"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..224
                     /locus_tag="ACG8MG_002673"
     CDS             <1..224
                     /locus_tag="ACG8MG_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0376658.1"
                     /translation="TTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDE
                     LKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            239..1108
                     /locus_tag="ACG8MG_002674"
     CDS             239..1108
                     /locus_tag="ACG8MG_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0376659.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1089..1823
                     /locus_tag="ACG8MG_002675"
     CDS             1089..1823
                     /locus_tag="ACG8MG_002675"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0376660.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1856..>1981
                     /locus_tag="ACG8MG_002676"
     CDS             1856..>1981
                     /locus_tag="ACG8MG_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HFQ0376661.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQD"
BASE COUNT          698 a          278 c          434 g          571 t
ORIGIN      
        1 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
       61 ttgctagacg tattggccag actccacaga atttcaataa aaaattacaa cgagaaacag
      121 taaccttgga tgagttgaag gccattgctg atgtgctagg tgtcaagttt gtgcaggcat
      181 ttattttacc agatggagaa gaaataaaaa tatctaacga ataaaggagg atgtttatat
      241 ggtaggtaat gttattcaaa tagtaacaga gaaattatca tctttgcctt ttatagaagg
      301 catcgtatta gggggctcac gtgcaagagg cacccataca gaaaattctg atatagatat
      361 cggaatctat tacaattcag actcatttga cctgacagcg attaatcaaa ttgctacaga
      421 attggatgat aaaaatagaa acaaccttgt tgtgcctccc ggagcgtggg gtgattggat
      481 taatggcggc ggatggttat ttataaatgg gtatcatgtt gacttgattt tacgtgatat
      541 aaaacgggta gaacaaataa tcaaagatac ggagcaagga attgttactg ccaattatca
      601 gaccgggcat ccccatggtt atataagtgc aatgtatcgt ggagaattag cgattagtaa
      661 aatactatat gctaagaacg aaagcttatg cgaattaaaa aagcaggcag aaatttaccc
      721 tactgctcta aagaaaagct tgatgaactt ttttatattt gaagctgaat tctctttaat
      781 gtttgtaaaa gcaaatgcgg gggtagagga taaatattat attgcaggcc atgtttttcg
      841 tattatttca tgcttaaatc aagtactatt tgcatgtaat aatgcttatt gcattaacga
      901 aaagaaagct ataaaactgc ttgaaacttt tgaatataaa cctgaaaaat atgccgaaag
      961 agttaatcat atttttgaag tactcggtct ttctcttttt gaatgctacg atatgaccga
     1021 gaaactttat aaagaagtga atgaaattgt atcggagata aataactttt taaacgagga
     1081 gagttcagat gaaagaaaac aaatatgatg ataatatatt ttttcaaaaa tacagtcaaa
     1141 tgagtcgctc gcagaaagga ctggctggtg cgggagaatg ggagactttg aaaaagatgc
     1201 tacctgattt taagggtaag cgtgtgcttg atttaggatg cggctatgga tggcactgta
     1261 tatatgcgat ggaaaacggt gcttcctctg tagtaggtgt tgatatttct cataaaatgc
     1321 tcgaagtagc aaaaggaaaa acccattttc cacagattga atatgaatgc tgtgccatag
     1381 aagatgtgga tttcccagag gagagctttg atgtaatact aagttcgctt gcgtttcatt
     1441 atgtagcaga ctatgagaat ttaataaaaa agatatatag gatgctgaag gctggtggca
     1501 atttagtttt tacagttgaa catcctgttt ttactgctca tggaacacaa gactggtatt
     1561 ataacgaaaa aggagaaata ctgcatttcc cggtggacaa ttattattat gagggcaaac
     1621 ggacagctat gtttttggaa gaaaaggtta caaaatatca tagaacactg accacatatc
     1681 taaatacact gctttcaaat agttttataa taaatcagat tgtggagcca cagccgccag
     1741 agaacatgat ggatattccg gggatggcgg atgaaatgcg acgcccaatg atgctgattg
     1801 tatcggcaaa aaagaagatg taataatata gaaaaaataa acgaggagta tgtaaatgag
     1861 atcagaaaaa gaaatgatgg atttagtact ttctttagca gaacaggatg aacgtattcg
     1921 aattgtgacc cttgaggggt cacgcgcaaa tattaatata cctaaagatg aatttcagga
     1981 t
//
LOCUS       DAZKNH010000182         1979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000182 DAZKNH010000000
VERSION     DAZKNH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.187"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACG8MG_002677"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACG8MG_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(728..1744)
                     /locus_tag="ACG8MG_002678"
     CDS             complement(728..1744)
                     /locus_tag="ACG8MG_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0376662.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAZKNH010000183         1930 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000183 DAZKNH010000000
VERSION     DAZKNH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.188"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..1216
                     /locus_tag="ACG8MG_002679"
     CDS             47..1216
                     /locus_tag="ACG8MG_002679"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0376663.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(1483..>1930)
                     /locus_tag="ACG8MG_002680"
     CDS             complement(1483..>1930)
                     /locus_tag="ACG8MG_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376664.1"
                     /translation="NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDG
                     QVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVK
                     SESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT          682 a          329 c          297 g          622 t
ORIGIN      
        1 agctttattc tttctgtaaa aaagaaagga gactaaataa actattatga aaaaaggaaa
       61 acatgctaaa aaaagaaaat attcattttt aatgatcgtt gtcgtgttgt ttagccttag
      121 tgcgttctct acaggaacta ttcttggatg gcaattttac aataatgaac actctcagat
      181 agttgcccat gctcaatcag ctcaaaaaaa gttgatactg actaccaatg aacaacagct
      241 cgttgaaaaa atcgatcaac tattaagaaa aaatgattat attggctcaa tttacgtaaa
      301 gaaaaataat cgcatcatca tggaaaaagg ctatggctat gcgaataaac tcacgcaaca
      361 accaaatgat ccttctcttt attatcagat tggttcggtt caaaaagcat tgactactct
      421 tttgatttta aaacaagtcg agcgtgggca cctttcttta gatactaagc tggctcaatt
      481 ctatccacaa attgctggca gccagcaaat tactatccgt gacttgctgt atatgcgctc
      541 aggcttgcac cgaactgctt ctccaaccat accgatgact gatgaagaag tcgttcaatt
      601 tgcgattcag cacttaaaat ttacagatta tcagacctct cattatgatt cattgaactt
      661 taccatattg actggaattt tgatcaaatt aacaaatcaa ccgtatgaaa aattattgac
      721 cgatgatatc atcgaacctt taggattaca gcatacaaaa ttttatgacc aagtcaaaaa
      781 ttcatccaat catgcactat cctatcaaat gtctaaagag gatgattact ttaaaccact
      841 gacagaatct gaaacagaga tccataacga attagggact ggtaatatca gtatgtccgt
      901 ctatgattta aatacgctgt ttacacaagt tttatctgga aaactagttt ccaaagactt
      961 actcttctca ttatggcgtg aaaatgcgaa tggacatcca tatagtggtg gggtatatag
     1021 cggagataat tatatcctgt cgcaaggaaa tatcgactct tttgatacat ctgctatatt
     1081 tagcaaagat tcacaggatg ctatcatcat ggaaagcaat ataaaagctg acaaaactat
     1141 caaactgcct actgctgatc taagaaacca aatctatgac ctgatggaag gaactactaa
     1201 gttggcaaat agctgatatg atgaaaagat agcaaatact acttctttga atagcatttg
     1261 cttaaaaaaa gtactcgact aaacgtagga aagagtgtgg gacaaatcta aaaatcaatt
     1321 tttgctacag actttaattc caaataaaca gaacagaaag aggtgttctt ctttctgttc
     1381 tgtccaaatt attctaagat ttctctttac caacattatt tgacaaaggg cctattaaat
     1441 ctaaaatagt atctttagat tgctaaaata tgtgctagca aatcattcat ggcttgttct
     1501 tatttttaat tttatgcaat aaaaaagaag aacaattagc gtgattatag aaataaataa
     1561 tgagatttta gaaaaaaatg tgtatgaata cctgattgtt actttattta ttccactttc
     1621 actttttact gtaacaaaag gacctccttg ttttagtact gtagcatttt ttgaattaac
     1681 agtaaccgtt aatcctttat accggaatac aggtaaaact attgttttgc cttttgaaga
     1741 tttaaacgta aatgtaattt catctttatt tggagaaaag ctactataaa taacttgacc
     1801 atcttctgta acttgatggt ttttcaaaag caattgatct tttttatagc ctataggtac
     1861 ataatcaaaa ttctcaaatt cttcaagttt attttccatt tgttcattag aataagttcc
     1921 ttgaaaattc
//
LOCUS       DAZKNH010000184         1887 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000184 DAZKNH010000000
VERSION     DAZKNH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.189"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(94..1134)
                     /locus_tag="ACG8MG_002681"
     CDS             complement(94..1134)
                     /locus_tag="ACG8MG_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0376665.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          655 a          282 c          231 g          719 t
ORIGIN      
        1 tattttgtaa tattcgtaat ataattagaa taataaaaaa tttttaattt taatgttact
       61 ccatgaagca acaatcactt tgtcagtaaa gtttcactct aaccagtctt tcgtaatagt
      121 gcttggggag cttgcataat tcaaacgttt ttcacttgat actttagcag ctattggttt
      181 ttcgataaac caatttttta catagacaaa aatcaggttc tggatatttt cgactttttc
      241 ggtttttaat ttttgatagg cttttaacag tgtcgtgtat aattcttgtt ctgcattgac
      301 ataataacta tcgagttctt caaaaactat cgaaatatca ctttctattt cagctttttt
      361 ctttgcatta tgaatagtct ggacggtttt ttgagcttca ctgtaacttg aagaaaatgt
      421 ttttatcaaa tttaatactc gttcagggac aaacgtagca atgctgtcgt ttgtcataat
      481 tgtctcaaaa ttgtctaaca acaattgatc ttgtaggcgg tctttttcag tgtctatatt
      541 gtgtctatta gtgtctaata tattattatt aagttctggt atgattttca taccctctga
      601 tttatcaacg ttttggcacg gtatgatttt cataccctct tgagccccaa gggattcagc
      661 gtctgatttt tcgtcgaggg tgggtatgat tttcataccc tctgatttat caacgttttc
      721 cctattctct ctatcaaatt tttcgagtaa gtagatatca ttttctgaga cttttaattc
      781 tgctagatat agtctattag gattattttt ccctaactgt ttgttaaatc ccatctgctt
      841 ttgtaataat aaaccaaagg attccaactc ttttttgatt gctatcgctt tttgtttttg
      901 gcagtttaat actctacata gttcatttgt cgtataagta aaataaacat taccttcttc
      961 atcgatttgc cgtttgctga ttgcgatctc taatcttgct tttaaaagca tataggcaat
     1021 cttagttgaa tctttcattt ttttgtattc gtcagaaaca ataaagactt ttggaaattg
     1081 ataaaataat tcgttgtaaa ctctatccgc cttaaaaaat ttaaaatcag tcattgtaga
     1141 tcctctactt tcacgttgtt ttatttatgt aaaattacct actcttttcg aaaatagcaa
     1201 agataaataa aaaatgcaaa cgcattaaaa ctgtttttta attaaatact acctaaaatc
     1261 agaaataaaa tgaattatgt actaccttta aagtcattaa gaactacttt aaataaatat
     1321 tatttataat taaatactac cagaaaaacg tatgaataaa atttagtttt aaaattaaag
     1381 ataattaagt actaccagtg tcaaaacaaa aaataaatta agaactacct tatgcatata
     1441 aaacatacta ccattactag taatatatac taccttattt cagtagtata tattatatta
     1501 ttcttataag acaaggcttt aggctagaaa acaatagatt atatactact aaaaaataca
     1561 ataagtacta ccaatgtcag atatatataa taattatgta ctaccattaa aattaactta
     1621 tttaataaga tactactgtg ttaatctgaa aaaaattact ttaaagtagt tacatactac
     1681 ctgttaattt agatactacc attaatactt aaattagata ctacctatat ttatattaag
     1741 tactaccgtt ctaagaataa gataataaaa aaatactacc aaattgcaat taaatactac
     1801 ttaaaatgta aaaaataaga actacccaaa ttaggtagtt cttaataaga gaatatcaga
     1861 taaatttgta caagtcataa aaaaatt
//
LOCUS       DAZKNH010000185         1850 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000185 DAZKNH010000000
VERSION     DAZKNH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.190"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..734)
                     /locus_tag="ACG8MG_002682"
     CDS             complement(33..734)
                     /locus_tag="ACG8MG_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0376666.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(750..1190)
                     /locus_tag="ACG8MG_002683"
     CDS             complement(750..1190)
                     /locus_tag="ACG8MG_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0376667.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1190..1765)
                     /locus_tag="ACG8MG_002684"
     CDS             complement(1190..1765)
                     /locus_tag="ACG8MG_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFQ0376668.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          518 a          400 c          286 g          646 t
ORIGIN      
        1 atttttcttt cccgtcgttt ggttttttat ttttagccta attcttgacc ttttgcatgc
       61 tggttatccg taaaatattt tccagcaggc tttccttcca attcagctaa tttttcttgg
      121 atctgacgga tcaccttttt tgcatcttct ggattttcta caaaatcgaa tttatcccca
      181 tccacttgta ttttaggaca tacatcaaaa tcttcatacc attgattata gcgggtgttc
      241 aacgtttcat aataggtata taacgtctca tcgctttcta gctgttcata gtcacgccca
      301 cgttttttga tacgttctag catcgtgtcc aaagaaactt tgatatggac catcaaatca
      361 ggccgttttt ttggagcaac atcatctaac tcgtttagca tcgtatctaa taagctatcg
      421 tactgctgta cttcgatatc cgttacccga cctaaatcag cgtttagatg gaacaacagg
      481 ctatcttcat agatggatcg gtctagcaca ttgtcatcgt tcactaaagc atttttcatt
      541 gctaaaaatc gcttgttcaa aaagaagatt tgcaataaaa aagcgtactg ctcaggattt
      601 gaataaaaga gcggcaatac ttcgttatct tcgacatttt cataaaatgg tttagaatca
      661 atctcttttg caagcatatc cgtcaaactg ctcttacctg caccaatcgt tcctgaaatc
      721 aaaatcactg acatacaacc cttcctcttc tataagtact cgcctacgta agtttcaaca
      781 ggcaatgttt caaaatcata ttcacgtaca gcttggtctg attttaaata cgtatggaga
      841 ctttgtgtaa tcatcaagtt cgtaggtacc gtctcttctt gtacaatgat catcggtttt
      901 ttcaacatag ttgccacacc taattcgtaa gccgttcctg gatcgattgt ttgatgctca
      961 aagtcaagga tagcaacaac aaattctgca tttgtcagtt cttccatatc tttttcgtat
     1021 acttcctgcg cccatccagc gctgaatagt tcataattgc tttcttggtg atgacgtgga
     1081 gagtagaaac ttgtgactgt tttgttttcc tccaacgctt tttcgatacg acttactcga
     1141 tcgatttgct cctctgaaaa aaatggtcct gctaaatata tttgactcat tattttgcct
     1201 ccttcaataa taactgattt ttaatagata ctgctcgtaa ttcattgacc agttccaatg
     1261 gattgttgga aaagattact tgatccaacc attttttatc cataaaatcc tcttttgcaa
     1321 aagtttctaa taattgttgc aagccgctgt aataaccgcc aatattcaat attccgattg
     1381 gcttgtcggg cgtcagaccg attgctatcc aagaaagaag ttgtgagaat tcctccaatg
     1441 tacctagacc tccagggaaa acgaggacag catctccctt ttcaaataat aatacctttc
     1501 tttcatcgat cgtttctgta ggaataaatg tagtaacatc ttttccaggc aattcagttt
     1561 caaaaagccc attcggatag atacctgtga cttttccgtt attttccagt actgttttac
     1621 taatcactcc cataatcccg gagtatgaac caccgtaaac cagttcgatt tcttcttgtg
     1681 ccatgtattt tcctaacgtt tgcgcaataa atttgtaact ttctttatta ccaaaacggg
     1741 aaccgcaaaa aacagtaagt ttcataaaaa aacactccct aactcaaaag tctatcttca
     1801 ttctacacgt gtttttttaa aataaaagac tagattttgt agttttttag
//
LOCUS       DAZKNH010000186         1833 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000186 DAZKNH010000000
VERSION     DAZKNH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.191"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACG8MG_002685"
     CDS             <1..969
                     /locus_tag="ACG8MG_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFQ0376669.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="ACG8MG_002686"
     tRNA            1159..1232
                     /locus_tag="ACG8MG_002686"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          605 a          317 c          346 g          565 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg
     1321 agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatact
     1381 tctgtatatt tgaaaagttt tactttgatt tttaaataga aagacatttg ggttaaattg
     1441 tgagataata ataaagaaga gtttaaagcg caccccaaac cacttcccca taagtgtgtt
     1501 acgctttaaa ctcttttata tttgaagcca ttaaaaagca taccatattt ttgaaaaaaa
     1561 gtgagaaaaa aggcttacaa ttggagtggt agttaattag tgacttattt ttgattttat
     1621 agcactgata ctataaaata tagatatcat catattacac aatcttaata ctaacttaaa
     1681 aaatatctcc tttcacaagt atggtgataa aattcgttcc gggctacctt tttaggtagc
     1741 ctactttaat ctttatacct ttctggatca acgaaagtat actttatata gtcataacgc
     1801 cgatgatcgc tacgtgcgtc cggcacgtca gtc
//
LOCUS       DAZKNH010000187         1828 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000187 DAZKNH010000000
VERSION     DAZKNH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.192"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACG8MG_002687"
     tRNA            complement(20..91)
                     /locus_tag="ACG8MG_002687"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACG8MG_002688"
     tRNA            complement(113..200)
                     /locus_tag="ACG8MG_002688"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACG8MG_002689"
     tRNA            complement(225..298)
                     /locus_tag="ACG8MG_002689"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACG8MG_002690"
     tRNA            complement(312..382)
                     /locus_tag="ACG8MG_002690"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACG8MG_002691"
     tRNA            complement(384..459)
                     /locus_tag="ACG8MG_002691"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="ACG8MG_002692"
     tRNA            complement(478..551)
                     /locus_tag="ACG8MG_002692"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="ACG8MG_002693"
     tRNA            complement(564..653)
                     /locus_tag="ACG8MG_002693"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="ACG8MG_002694"
     tRNA            complement(677..750)
                     /locus_tag="ACG8MG_002694"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="ACG8MG_002695"
     tRNA            complement(764..837)
                     /locus_tag="ACG8MG_002695"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="ACG8MG_002696"
     tRNA            complement(865..937)
                     /locus_tag="ACG8MG_002696"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="ACG8MG_002697"
     tRNA            complement(957..1030)
                     /locus_tag="ACG8MG_002697"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
     gene            complement(1040..1113)
                     /locus_tag="ACG8MG_002698"
     tRNA            complement(1040..1113)
                     /locus_tag="ACG8MG_002698"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1077..1079),aa:Arg,seq:acg)
     gene            complement(1124..1209)
                     /locus_tag="ACG8MG_002699"
     tRNA            complement(1124..1209)
                     /locus_tag="ACG8MG_002699"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1173..1175),aa:Leu,seq:taa)
     gene            complement(1226..1297)
                     /locus_tag="ACG8MG_002700"
     tRNA            complement(1226..1297)
                     /locus_tag="ACG8MG_002700"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1263..1265),aa:Gly,seq:gcc)
     gene            complement(1309..1381)
                     /locus_tag="ACG8MG_002701"
     tRNA            complement(1309..1381)
                     /locus_tag="ACG8MG_002701"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Thr,seq:tgt)
     gene            complement(1399..1480)
                     /locus_tag="ACG8MG_002702"
     tRNA            complement(1399..1480)
                     /locus_tag="ACG8MG_002702"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1444..1446),aa:Leu,seq:tag)
     gene            complement(1488..1560)
                     /locus_tag="ACG8MG_002703"
     tRNA            complement(1488..1560)
                     /locus_tag="ACG8MG_002703"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1525..1527),aa:Lys,seq:ttt)
     gene            complement(1564..1636)
                     /locus_tag="ACG8MG_002704"
     tRNA            complement(1564..1636)
                     /locus_tag="ACG8MG_002704"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1601..1603),aa:Val,seq:tac)
     gene            complement(1650..1765)
                     /gene="rrf"
                     /locus_tag="ACG8MG_002705"
     rRNA            complement(1650..1765)
                     /gene="rrf"
                     /locus_tag="ACG8MG_002705"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          483 a          504 c          428 g          413 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaattaac gcgcccaaga ggagtcgaac ccctaacctt ttgatccgta
     1081 gtcaaacact ctatccagtt gagctatggg cgcatatttg aaatgccgag gaccggaatc
     1141 gaaccggtac ggtgatcact caccgcagga ttttaagtcc tgtgcgtctg ccagttccgc
     1201 caccccggcg cgattgcttt ggcaaagcgg aaaacggggt tcgaacccgc gacccccacc
     1261 ttggcaaggt ggtgctctac cactgagcta tttccgcgtg tttaaagatg ccggctaaag
     1321 gacttgaacc ctcgaccctc tgattacaaa tcagatgctc taccaactga gctaagccgg
     1381 catgtaaaac ttaaattatg cgggtgaagg gacttgaacc cccacgccgt aaggcgctag
     1441 atcctaaatc tagtgcgtct gccaattccg ccacacccgc aaaaatatga gccgtacagg
     1501 gctcgaacct gtgaccctct gattaaaagt cagatgctct accaactgag ctaacggctc
     1561 atatggaggt taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca
     1621 gctgagctaa acctccatgg ataacttaag cgtggcgacg tcctactctc acaaggggca
     1681 acccctcact acaatcggcg ctaagaagct taacttctgt gttcggcatg gttacaggtg
     1741 tatccttctc gctatcgcca ccacactgtg gtgttatctt ttattgagta aattttgttc
     1801 actcaaaact ggatttgaag ttacataa
//
LOCUS       DAZKNH010000188         1766 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000188 DAZKNH010000000
VERSION     DAZKNH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.193"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..189
                     /locus_tag="ACG8MG_002706"
     CDS             <1..189
                     /locus_tag="ACG8MG_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0376670.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1480)
                     /locus_tag="ACG8MG_002707"
     CDS             complement(1055..1480)
                     /locus_tag="ACG8MG_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HFQ0376671.1"
                     /translation="MHRKVRITRKSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISI
                     EGILRIQGPAFIAQGAHIDIRKNAVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDT
                     LFMDTDSHTIFELNTHRILNKNNKIVIGKHCWIGAKSVI"
     gene            complement(1582..>1766)
                     /locus_tag="ACG8MG_002708"
     CDS             complement(1582..>1766)
                     /locus_tag="ACG8MG_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0376672.1"
                     /translation="SSIVYGGKIAFYFSAIIEFLQLFLRLGTFQISDFVYNTLGGILG
                     VILYKLILITKRDGER"
BASE COUNT          562 a          349 c          209 g          646 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat
     1741 ttttcctcca tatactattg aggacc
//
LOCUS       DAZKNH010000189         1626 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000189 DAZKNH010000000
VERSION     DAZKNH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.194"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..>1626
                     /locus_tag="ACG8MG_002709"
     CDS             275..>1626
                     /locus_tag="ACG8MG_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0376673.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     "
BASE COUNT          585 a          228 c          294 g          519 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattg
//
LOCUS       DAZKNH010000190         1536 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000190 DAZKNH010000000
VERSION     DAZKNH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.195"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1536)
                     /locus_tag="ACG8MG_002710"
     rRNA            complement(<1..>1536)
                     /locus_tag="ACG8MG_002710"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DAZKNH010000191         1524 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000191 DAZKNH010000000
VERSION     DAZKNH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.196"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1480
                     /locus_tag="ACG8MG_002711"
     CDS             14..1480
                     /locus_tag="ACG8MG_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFQ0376674.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          417 a          289 c          324 g          494 t
ORIGIN      
        1 tggggtatag attatggaaa aacagcaaga caagggcttc ttcgggcagc cgaagggatt
       61 gtcaactttg ttcttcactg agatgtggga acgatttagt tattacggta tgcgtgcatt
      121 acttatctat tatatgtacg atacagttgc caacggggga cttggattac cacgagcaac
      181 agcattagct atcatgtcga tctacggatc tttgatctac atgtctagta tcgtcggcgg
      241 atgggtatct gaccgagtgt taggagcaaa acgcactgta ttcttcggtg gagttttcat
      301 tatgcttggc cacatcgtct tggctttacc atttggtgta agtacattgt ttgtttcgat
      361 ggcattgatt attatcggaa ccgccttttt gaaaccaaat gtttctggca tggtcggaca
      421 tctttattca aagactgact tgcgaagaga tgcaggattt tcgatttttt acatgggtgt
      481 caacttaggg gcattattag caccactaat cgttggtaca atcggtcaag aatataacta
      541 tcatttaggg ttctctttag ctgctattgg gatgttcttt ggattattac aatacatcat
      601 ccaaggacgc aagacattag acggagtggg tacaacaccg ccgaatcctt tgacaactga
      661 agaacgtaaa ggattcatcc gtaacttgat catcgcactt gttgttatcg ctgcagtatt
      721 cggcggggca caactaactg gtcatctgac aattgacttt tttgtcaata caatcagtgt
      781 attggggatt atcttaccgt tgtattactt tatcaaaatg ctgacttcaa aagatgtaac
      841 tgctgaagaa aaaccaagag tatgggctta tatcccatta ttccttgcag caatcatttt
      901 ctggtcatta caagaacaag gttcatctat cttagcttta tttgctagtg aacgtacaca
      961 atctacgcta tttggtgtaa cgatccaagc aagctggtat caatcattga atccaatttt
     1021 catctttatt ctgacaccag tattcgtaac gatgtggacg aaacttggtg aacgtcaacc
     1081 atctacagta gtgaaattct ctcttggact tttatttgca ggattatcgt acttgctatt
     1141 gatgttgccg ggtatgcttt atggtacaag tggaaaagta agtccattgt ggttgttggg
     1201 aagtttcttc atcgtggtta ttgctgaatt gtgtctttca ccagttggtt tgtctgtgac
     1261 aacaaaatta gcaccacgag catttgaatc acaaacaatc gctatttggt tcttagctga
     1321 tgcttcttca caagcaatca atgcacaaat tgcacgcttc tacacaccgg gaacagaatc
     1381 tgcttacttc ggtattatcg gtgcagtagc aatcgtcggt ggtatcttgc tatttactgt
     1441 gaaagaacca atcaaaaaac taatgggctc tattcattaa tcaatgaaca taaaaacagg
     1501 aattgcttat ttgcagcagt tcct
//
LOCUS       DAZKNH010000192         1493 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000192 DAZKNH010000000
VERSION     DAZKNH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.197"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            735..1121
                     /locus_tag="ACG8MG_002712"
     CDS             735..1121
                     /locus_tag="ACG8MG_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0376675.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1215..1406
                     /locus_tag="ACG8MG_002713"
     CDS             1215..1406
                     /locus_tag="ACG8MG_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0376676.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          552 a          229 c          206 g          506 t
ORIGIN      
        1 acatgaagag actttaataa gacttcttta tctgaaaaca cttttgtttc tctctttcct
       61 ttctacatca ttaaatagta ttttatacgt tatctaaaat actattcctc tgtcaaaata
      121 agctaactta cttcattata aaatatccac ttatcgcttt catgtatatt ccaccgtaaa
      181 aagtacattt tatcaccgag aactcatcat ttttcatcat gatgaatact atttacatat
      241 atctagttca aagacaataa ttattatgat ccgttattat ctaagcacta tacagataat
      301 aggaattttt cgtttctaaa tattccaatt tgggaatcca ataaaaattt tcgttcaaat
      361 acgtgtctat tcgaactctt ttctagttgt taattatgaa gggggaagaa ataaaaaata
      421 caatctatac acgaataaga atattatcca aaaggaggtt ttagaaaaga tttattatac
      481 gtaaaaagct caagacattt tcttagccat aaggacatgt ttgatattta agaagatata
      541 tgaattttct tttctaagta taaagataac tatccattat caactaaaaa aacatttttg
      601 aaaataaaca tcagcagtcc tatcaatgca tgtgagctta tagaagttat tcgcagcgca
      661 gcataattgc attagcttct tttcgtgttt tttgcctctt ttccctttaa atcttaatgg
      721 aggaggtgtg caatatgtca gataacgacg aacgcgtaaa tgcccagttg attcgattct
      781 ttgaaaaggt cattttgaac accgctaata cttattataa aaaacaagaa gaaattcaaa
      841 aacatgaaca acttgatcaa ctcccaccat atttgtttac taaagatact ataaaattta
      901 aacaaccgat taatgtttta aatgtgactc tccttgttga agatataaaa ttagcaaaaa
      961 tccttccttt gttgaaagaa cctgaacaaa tttttcttat cgaaaaattt atctttgata
     1021 aaactgataa agaaattgga aaggttctgg ggattacaag acagggtgcg acaaatttaa
     1081 aacatcgact ttacaaaaaa attcaaaaaa tgctggttta atatctgcat aataactaat
     1141 tttgtatgct tctttgctcg ttctcctcct tcccaaagaa atagattata agtccatggg
     1201 aagaaggtag atttatgaat tctttaaaag aagatttcat tttagcaaag gctggaaatg
     1261 aagaagctgt tgaggcaatt ctcaaaagat ttagctcttt gattcataag caatcctgga
     1321 gaactggaaa atatgatcaa gattgttatc aagaatgtat gcttgcaata tatctagcta
     1381 tctcgaaatt cgaaattaaa gaataactca actaagggag tctgtgacat aagtaaaaag
     1441 atagtttcaa atttcgaaca ataaatttga aactatcttc tttataatta taa
//
LOCUS       DAZKNH010000193         1482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000193 DAZKNH010000000
VERSION     DAZKNH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.198"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..828
                     /locus_tag="ACG8MG_002714"
     CDS             <1..828
                     /locus_tag="ACG8MG_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0376677.1"
                     /translation="NTEVMEATIVENGLPYDLSECKVYFESVVGGKYPVQLETKIVDA
                     KKGKINYIFDKYSMQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSYW
                     QSIEDLIADMTDFINENKGDFTDWMNERKEEFERWRQEQEHSFQDWREGQESDYLAWF
                     ESIKDILKTIDPGGVMLAELTDARVDIQGVRHASISERLLADMDYLYQKLRATLFTIE
                     YGEIEVTDILQDDLFSDNHEVEKVGTVAFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            828..1448
                     /locus_tag="ACG8MG_002715"
     CDS             828..1448
                     /locus_tag="ACG8MG_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376678.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          529 a          212 c          337 g          404 t
ORIGIN      
        1 aatacagagg ttatggaagc gactatagtt gaaaatggtc ttccctacga tttatcagaa
       61 tgtaaggtat attttgaatc agttgtaggt gggaaatatc cagtccaatt agaaacaaaa
      121 attgtggatg ctaaaaaagg gaaaattaac tatatttttg ataaatattc catgcagtgt
      181 ttacatcgac aaacagccaa tttcattata tttaaaggag aagacttgat tggaacaact
      241 caagacttct cttattttgt cattaatgct gtttcaaaaa cagaaggaga aatgggttct
      301 tattggcaat caatcgaaga tttaattgcg gatatgacgg actttattaa tgaaaataaa
      361 ggcgatttta cggactggat gaatgaaagg aaagaagaat ttgaacgttg gagacaagaa
      421 caggaacaca gtttccaaga ttggagagaa ggacaagaat cagactattt ggcatggttt
      481 gaatcaatca aggatatttt aaagacgatt gatccaggtg gagtcatgtt agccgaacta
      541 acggatgcgc gagtagatat tcaaggagtt cgtcatgcgt ctatttcaga gcgtttgctg
      601 gcagatatgg actatttata tcagaaattg cgagcaacgc ttttcacgat tgaatacggg
      661 gagattgaag tgactgatat tttgcaggat gatctctttt cagataatca tgaagtcgaa
      721 aaagttggaa ctgtagcatt cccgattgaa gaaggagcct tgatcatcgc aaccgttgat
      781 gatccaaagc aaaatgtgtt cactcttgag aaagttgggg tgatctaatg gctaaaacta
      841 aacgaatgat ggaaacggat gaaaaaacgg gtgtacaacg ccaattcttt ccaatcacac
      901 atgcttccgc agttcttggg ttagaagaaa taatagcagg agaagcaaca gttttatctg
      961 ttaatggaaa aattggagcc gtcgtcatta ctaaagaaga tttgggatta gagaatgttc
     1021 tcacagaatt accctatgca agtgaagaag atgacggcat tatcacggca gaaatgtatc
     1081 aaaaaatttt aaacagtgga gaaggtgact acgtgttgcc agttgccact atcgaccgtt
     1141 tgggtggcat aaaagttggt gaattattga cgattgatga gacagggaaa gtctctgcag
     1201 tcagacaatc tgatgtcaat ttttcgctgg agttaaaaga aaaactcgat tcactgaaaa
     1261 attatactgc tggagaaaac atcactattg atgaagatgg aacaattagt tctacaggtg
     1321 gtagtgggac tggtggtgtc aatcagtcat atgtagatca aaaattccaa gaagctgtaa
     1381 atcaagcaga aaattacaca aacgaaagaa ttccaaattt tacttttgaa aaaattgggg
     1441 aggtatagag aatgacagat atcgtaaaaa taaaacaaag ca
//
LOCUS       DAZKNH010000194         1479 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000194 DAZKNH010000000
VERSION     DAZKNH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.199"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..828
                     /locus_tag="ACG8MG_002716"
     CDS             <1..828
                     /locus_tag="ACG8MG_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0376679.1"
                     /translation="NTEVMEATIVENGLPYDLSECKVYFESVVGGKYPVQLETRIVDA
                     KKGKIKYIFDKYSMQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSYW
                     QSIEDLIADMTDFINENKGDFTDWMNERKEEFDRWREEQENSFQDWREGQESDYLSWF
                     ESIKDILKSIDPGGVMLAELMDARVDLQGVRHESISERFLSDLNYLYQKMKAALFTIE
                     YGEIEVTDILQDDLFSDNHEVEKIETVEFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            828..1445
                     /locus_tag="ACG8MG_002717"
     CDS             828..1445
                     /locus_tag="ACG8MG_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376680.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          544 a          192 c          311 g          432 t
ORIGIN      
        1 aacactgaag ttatggaagc tacaattgtc gaaaatggtc ttccatatga tttatcagaa
       61 tgtaaggtat attttgaatc agttgttggc ggaaaatatc cagtccaatt agaaacaaga
      121 attgtggatg ctaaaaaagg gaaaatcaaa tatatttttg ataaatattc catgcagtgt
      181 ttgcatcgac aaacagccaa tttcattata tttaaaggag aagacttgat tggaacaact
      241 caagacttct cctattttgt catcaatgct gtttcaaaaa cagaaggaga aatgggttct
      301 tattggcaat caattgaaga tttaattgct gatatgacag attttattaa tgaaaataaa
      361 ggcgatttta cggactggat gaatgaaagg aaagaagaat ttgatcgctg gagagaagaa
      421 caggagaata gtttccaaga ttggagagaa ggacaagaat cagattattt gtcatggttt
      481 gaatcaatca aggatatttt gaagtctatc gatccaggtg gagtaatgtt agccgaatta
      541 atggatgcac gtgtagatct tcaaggtgta cgccatgaat cgatttctga acgtttccta
      601 tccgatctaa attatttgta tcaaaaaatg aaagcagcac ttttcacgat tgaatacggt
      661 gaaattgaag tgactgatat tcttcaagac gatctctttt cagataatca cgaagtcgaa
      721 aaaattgaaa cagtagaatt tccaattgaa gaaggagctt tgattattgc aactgttgat
      781 gatccaaaac agaatgtttt cactcttgag aaagttgggg tgatttgatg gttaaagtaa
      841 agagaatgat ggagactgat gaatctggtg ttaaacgtca atttatgcca attacacatg
      901 tttctgcagt tttaggctta gaaaaaatga tatcaggaca atcaaaagtt ctttcggtta
      961 atggtaaata tggtgcagtc attttaacga aagctgattt aggattagag aatgcaatta
     1021 ctgaactacc ttatgcaagc gagacaagcg atggtattct tactgctgaa atgtttcaaa
     1081 agatcgtaaa tggagaaggt ggaacatata ttttgccgat tgctactcct gaacaattag
     1141 gaggtataaa agttggggaa ctcttagaag ttactgaaga aggtgtttta tctgcaacta
     1201 agcaaacaga cttcaatttc tctgaagaat taaagggaaa attagaatct ctaaaaatat
     1261 taaaagcagg tgcaaatata tcaattgcag aagatggaac aattagttct acaggtggta
     1321 gtgggactgg tggtgttaat cagtcatatg tagatcaaaa attccaagaa gctgtaaatc
     1381 aagcagaaaa ttacacaaac gaaagaattc caaattttac ttttgaaaaa atcggggagg
     1441 tatagaaaat gacagatatc gtaaaaataa aacaaagcg
//
LOCUS       DAZKNH010000195         1432 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000195 DAZKNH010000000
VERSION     DAZKNH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.200"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..333)
                     /locus_tag="ACG8MG_002718"
     CDS             complement(<1..333)
                     /locus_tag="ACG8MG_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376681.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKK"
     gene            complement(335..>1432)
                     /locus_tag="ACG8MG_002719"
     CDS             complement(335..>1432)
                     /locus_tag="ACG8MG_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFQ0376682.1"
                     /translation="ADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHV
                     PTGTPGLLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMM
                     NFIVQIFYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLV
                     LLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISA
                     GRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNG
                     FLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVE
                     AHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          550 a          235 c          174 g          473 t
ORIGIN      
        1 ttttttgtaa acattggaga aaagtatttt tgtgaaataa ttttccccct gtataaaaat
       61 ttcatcataa acggataaat catccgagaa aaattgatta tggttagtta acaagttaat
      121 tttacgttta aaggaagaac cgattttccg gtgattcaca aaaacaacat cattaaatag
      181 ccctaattct tttatacgct cactatatct tctagcgttt tcaaattcag aataaataaa
      241 taaatctcct tttgattctc ggtaaaaatt ttgataaaca tttaatatat taatcaaatg
      301 taaaggagtc caagcaatga aagctgtctt catattaatt ctcctttttt aaatacaagt
      361 aagaaatgct tcccaaataa taccagaaaa caattgaaat gacatcgcgc ttattgaaaa
      421 gtacatgagc ctccaccatt tcatctataa aaatagatag tataaaaatt aaaacaatac
      481 caagaaacgc ataattcact ttacttctaa aaaatgtttt gagtaaacta aagatattta
      541 acacatagaa agcatagata catataaaag caattatacc acttgataca agtacttgaa
      601 gaaatccatt atgcatgttt ccatgtgctc tttttagttc attcacatct gtagtatcaa
      661 gatctacttt tttcaaaaat tttgcatcgc tattcccata gaaatcggtc tctccataac
      721 caaataacaa agttggtctt ataactttaa gcgcagcttt ccaaatcgta aaacgaccag
      781 ctgaaatttc ggagttatca ctagaatgct gtaatattac ttctttttct ggaagtggag
      841 ttttatctga ttcatttgac tctataactt ctacttttct atcaatcgat acaggaattg
      901 ttgctaaaaa atccttagca taattagtac caaaagaaaa atataccaat agaactaata
      961 atatgcttac catccatttg atttttacag gtagtacttc tctaatgctc tttctaaatt
     1021 ttgaattggc aaacaacaaa agaaataata ctaaaaaaga acaaattact acctgggcac
     1081 ctctagacga agaaagtgta atataaaata tttgtacgat aaaattcatc atataaaaaa
     1141 cacgaatcgt ttttgttatt tttgaaatta atacactaat taaacttaaa ataatagaag
     1201 tatatccaaa catagctcca acatttggac ttgtatagac accaaataac cgatgttcaa
     1261 taaaaccttg tcttgcaagt aatcctggcg ttcctgttgg gacatgataa gaaatcaaaa
     1321 aaataaattg atagatagat attagcgcag ctaaaaaaat tatgataatg aaaacattat
     1381 taaaatacac catttttttc tttttttcct cttcactttg tgaaaagtct gc
//
LOCUS       DAZKNH010000196         1423 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000196 DAZKNH010000000
VERSION     DAZKNH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.201"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..144)
                     /locus_tag="ACG8MG_002720"
                     /pseudo
     CDS             complement(1..144)
                     /locus_tag="ACG8MG_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(186..1312)
                     /locus_tag="ACG8MG_002721"
     CDS             complement(join(186..1084,1084..1312))
                     /locus_tag="ACG8MG_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0376683.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT          409 a          256 c          236 g          522 t
ORIGIN      
        1 ctccagataa gtgcgatact cagacgcatc attcagtaac aaccgaacgg ttgtcgtccc
       61 ccaacgaatg atcctagagg ggacgacaca aagacaatgg gaacactctt ctggcttgta
      121 cagaagagtt cctttgtaaa tcatggattt gagtcaatat cttggacaca ttttagtaga
      181 gacggttaag ccattttttc ttttgctaat tcttcaaatt cctgtggggt tagatagtga
      241 atcgagctat ggattcgatt tcggttataa aatccttcaa tgtagctaaa tagcgctcga
      301 tttgcttctt caaaatctga gtagctagtc gtgtagactt cttctttttt caatgaggca
      361 tggaaagatt cgattccagc attatcataa ggtgttcctt ttctactata agaatgcctt
      421 attttgttgg ttttaagcca ttgttccact tctcttgctg tgtattggct gcccaagtca
      481 gtgtgtagaa tcattccttc tggaatgtga taatgtattt ttgctttatt caatgtttga
      541 atgacacaat ccacagtcat tcgttttgaa aatgtataac taataatttt tttcgtgtgt
      601 aaatccatga ttgaagataa gtaacaccaa ccatttttct tagtaggaat gtacgtaatg
      661 tcagctgccc atttctcaca gatcgtttca gtagaaaagt cctgatttaa gagattctct
      721 tttgaaatga tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt
      781 aaatttaatt gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt
      841 aataaaactt gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt
      901 gaaatcttct ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta
      961 taaaaataat agacacttct agggatttct aataaccgac ataacaaaga aacagtgtaa
     1021 tccttacacc atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg
     1081 ctgcggcttt ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt
     1141 cagctacttc ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt
     1201 tgtaaatcgt tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag
     1261 tttgattcag ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc
     1321 atcctctcgt agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga
     1381 tactaacacc aagttatctt tagaaccatt tcagatacat atg
//
LOCUS       DAZKNH010000197         1417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000197 DAZKNH010000000
VERSION     DAZKNH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.202"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8MG_002722"
     CDS             complement(150..1337)
                     /locus_tag="ACG8MG_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFQ0376684.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZKNH010000198         1403 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000198 DAZKNH010000000
VERSION     DAZKNH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.203"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(167..1186)
                     /locus_tag="ACG8MG_002723"
     CDS             complement(167..1186)
                     /locus_tag="ACG8MG_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0376685.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1183..>1403)
                     /locus_tag="ACG8MG_002724"
     CDS             complement(1183..>1403)
                     /locus_tag="ACG8MG_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0376686.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT          397 a          289 c          253 g          464 t
ORIGIN      
        1 taaacaatca accagtagaa tgtgtggcaa caaacctgtt atcgcatagc ttggagtgtg
       61 actattatgg gtgatagtga agatatgcga taacatcact attttatcga atgattttta
      121 tagttgtcaa tatagttatg taatgctcct caacgaggag ctatttttat cgtttaggaa
      181 tatttaaata ccaacgtttg tcatggaaat cttgcgcacc gcctttagtg tttccctctg
      241 gatcattcgt tgcccgcatc atgacataga ctttcttatt agggaagtta cgcatgttaa
      301 aagatacatg atagccaaca tttccagaag tattataagc ttgatttaca tctgatctat
      361 aaattccatc agctcttact cgagctaatt ctttcccagt attgtaatcc ataatgaaga
      421 tatactcgta tttatagtta gcaatgtgcc atccagctac atgcaaattt gcattttcga
      481 tttctccaaa ctgatcaatg tgggcgtaat tcgttccatc tgtcagtgta ggattagctg
      541 caccagctcg tgttggatca atgacaggct tgttttcaga agttgttgga ttttcatcgg
      601 taaatccatg agctaaatca tatgctaatt tttctttact tacgcccatt tcagaaagat
      661 aaccgtaagg atctgtatga tcgccccaga tgttttgtgt tacccataaa tgcgatttga
      721 ttcccggttg gttataagga gtgtccaacg ttaatggaat accatatttc attgctgaat
      781 ctctagccaa ttcaacgtat gccttgtagt ttttctcaaa cgttgcttta tcatgtgtgt
      841 gttgtaactc aatctgcaca ggactgttgg cattagcata cgaaccagca ccgtactgta
      901 cataaccagg ttgaccgact tgataaacaa ttccgccgtc tcccacaata taagcagtat
      961 aagcgctagt ccatgaacgt tgcatatact gcgcttcatt gcgtcctgtt gctgtttcat
     1021 tagccgtttc atgcagtaaa atatactgat tatttgctac ttgtgagcta ccttcatttg
     1081 cgcccaaatt aaattcattg ttgatagtat aggcaaatcc gttaattggc aataaaaaaa
     1141 gagccgttaa caggctcatc gcagtaatag taattttctt tttcatttgt ttcctccttc
     1201 ttcgctttca gccgagaaca ttttgtaggt tcgatttgat acacccaaca cactccctaa
     1261 aaacgcgcca aaaccagtaa tgatgacaac acagatatct gtgtactgcc aattgagcgc
     1321 tttaccaact aaccccacga aagtagctag tgcgggaata attaccagtg cgaaccattt
     1381 tagtacttcg aacgttttat tat
//
LOCUS       DAZKNH010000199         1402 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000199 DAZKNH010000000
VERSION     DAZKNH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.204"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            484..570
                     /locus_tag="ACG8MG_002725"
     CDS             484..570
                     /locus_tag="ACG8MG_002725"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0376687.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(873..1097)
                     /locus_tag="ACG8MG_002726"
     CDS             complement(873..1097)
                     /locus_tag="ACG8MG_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376688.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT          414 a          288 c          233 g          467 t
ORIGIN      
        1 cttctgtatc cgttgggaga gtaaggctca actgccccct aaaaagggtt ctaaaaaatc
       61 tttaaaaaat aaataaaata aacgcgcgcg cgcgagcaaa atttcccgcc aaaaaatcac
      121 ccacttttta aaataaacag gttcgcgcat gatattaaac tgccaatcta gctccctatg
      181 gtcgctgtaa gaaggagtga tcgctacgct ctcacatttt acttgcagca gtcgccttta
      241 ggctccttcg ccaaaaataa ttcattaggg actcctttgt ggcgtcctga gagctcgtct
      301 aagccgttct aagacaagat tcgataaaaa actatgtcct tcgttctaaa gcgccttaca
      361 ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa aacttaggct tatttttgct
      421 tttttattgt ataattgatt gtcgcctatc cgacagtctt gcgactggaa ggaggtgtgt
      481 ttcgtgttca ctaactttat ttgctccctt cttgttgggc ttttaagcgc tttgttcagt
      541 tactggttga ataaccgaaa caagaagtaa aacttaatta aggcgactca gcccacaaaa
      601 aaagccccag agaatccgac ctctctgggg ctttcgtgtg tttcgtgttc acttatttgc
      661 aatcaaatta taacagaaat aaatatttat ttcaaaaata tcctacaaaa gtctaaaatc
      721 cgtaattatt tcttatatgc aacatttaca aataaagtcg ctctgagagc tcgtattttt
      781 cgatttaaga cgttttattt agtttttggt gtattttcat tggtccaagc taaaaaactc
      841 ttagaagcga ttttagagcc atttaaaccc acttaattat attctccatg aaattcgctc
      901 catgcgtcaa aagctgcgtc tgaaattccg ccgtcttcgt tcactagttg cattaatttt
      961 tccaacgatc gttttctttc gggtgtcata tttttatatt ccaacaattt ttcgtctgtt
     1021 aaatcgaact tgattgcttg aattgcttgc tctaattttt ctaagttttg aaaatattcg
     1081 taaggaaatt tttccatttt ttctttctcc agtctcacaa tttttctaga aataaaatca
     1141 cttctttgcc gtctatactc tcaaaaaaaa tgtcgtttga aaaactaaat aaatttttga
     1201 tcgtgcattt ttaaatttgg ttatcgatta cacgtgcagc actccccccc gaattttaaa
     1261 gagcataagg aaacgtgtac ccactttcta aaaaaagtgg gtagataggc tgtgcctatc
     1321 acgtttctca gaaaaaaatt tgatatcact ttttcgccat cgtgggtagc ttatgtatag
     1381 cattttggca aaaaacagct at
//
LOCUS       DAZKNH010000200         1391 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000200 DAZKNH010000000
VERSION     DAZKNH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.205"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            384..716
                     /locus_tag="ACG8MG_002727"
     CDS             384..716
                     /locus_tag="ACG8MG_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0376689.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            626..820
                     /locus_tag="ACG8MG_002728"
     CDS             626..820
                     /locus_tag="ACG8MG_002728"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0376690.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            859..1005
                     /locus_tag="ACG8MG_002729"
     CDS             859..1005
                     /locus_tag="ACG8MG_002729"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376691.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
BASE COUNT          490 a          246 c          245 g          410 t
ORIGIN      
        1 aacgaatgat tttaattatc aacgtttttt aattcgtttt tagcttaaaa ataacttttg
       61 tcacagtctc actcctaaaa ttactttatt tacgtatgaa gtgggcagga ggtcgaatcc
      121 agaactgggc tatggtactt caacgaggac ttctcctcta gaattgaaaa gcatgcgatt
      181 taccttccgt aacgacggta gatttattca actatgtgtc caatcaaaat ttgtctgatt
      241 tcaaaaatag tcaagagaat tttggttgtt aaaaactctt atctatcaac ttccacagtt
      301 tagttgacaa accctgatac taagttgggc tatgatacac ttttcttgac tatcagttac
      361 gttaccggaa ggaatgaact ctattgagaa aaacatatga tcgtaaattt aaactaaaga
      421 tttcacagga tatccttgag aaaaaagtaa ctaccaagaa aattgctgaa gaatacaata
      481 tctctcgtcc aacaatttcc cgatgggttt cagaatatcg tcgatacggg aaaaatgctt
      541 ttgctggaca aggaaaaaga ttacctgata aagcagattt ctacattctt gaacaggaaa
      601 ataaacgact aaaagaggaa gacgagtgtt ctacgaacac aaagatcgat acggatctcc
      661 agggatcact caaaaacttt acgatgaagg aatcgaaatc aataaacgtg ttgtagctgt
      721 tcttatgcgt gaaatgaatt tatgcacaag aggatttcat cgacgcaaat cttctcatgg
      781 tagagaaaaa acaatcaaag aaatggtaaa aaaatgttga atcgataatt tgaacagaac
      841 caaattgatg ctgtttgggt gatggatatt acttacattc catgtaatga cggtcgcctc
      901 tatttgtcca cctatattga cttagcaact cgtattctac gttactatat ggtagattca
      961 cacatgaaaa aagagattgt cgtccgtcca ttacaaattt attaaggaga attgcctacg
     1021 gtaattcatt ccgatagagg tagccagcat cggtctttta tttatcaaga gctgttgaac
     1081 gaccaccaca tcacacattc agtgagccaa actgggactc ctgttgacaa tgcagtaatt
     1141 gaagcgtttc atcgatcaat caaatgagaa ttaatcgact ccaacaaata taaaagtaaa
     1201 atagagatga aggtgttaat ccaagattat ttagaagact actacccaaa caaaagaatc
     1261 catacaaaat ttatgatgac tccttgacaa tttgaagcga agaaaataag ggatctataa
     1321 tatctagtgt tggtgacgcc ttctgttgcc aatacttttt cttttttcta ataaacgcaa
     1381 acagacacct t
//
LOCUS       DAZKNH010000201         1366 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000201 DAZKNH010000000
VERSION     DAZKNH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.206"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1366
                     /locus_tag="ACG8MG_002730"
     CDS             82..>1366
                     /locus_tag="ACG8MG_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFQ0376692.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLI"
BASE COUNT          466 a          263 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatca
//
LOCUS       DAZKNH010000202         1362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000202 DAZKNH010000000
VERSION     DAZKNH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.207"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..261)
                     /locus_tag="ACG8MG_002731"
                     /pseudo
     CDS             complement(82..261)
                     /locus_tag="ACG8MG_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1087..>1362
                     /locus_tag="ACG8MG_002732"
     CDS             1087..>1362
                     /locus_tag="ACG8MG_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0376693.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT          431 a          277 c          191 g          463 t
ORIGIN      
        1 ttaagtgaat gtttaatttg aatcatcgat tgatcgctat ctaattgcag cagcatcttt
       61 ttatgaagaa acaacttttt cttatctatc aacgtagaca ggtgttgctg tttcttctaa
      121 tagtgtactt aattccttaa tattttgttt tccttcttct aacatccaac tactcgcttt
      181 ttcttcgcct tcggtaacaa aaggcgccac tcccttcgcc catgttgctc gcccacataa
      241 gacaccattc tccataaagg acaacgcgga gataccaccc atctaaataa catccataaa
      301 cctttctagt agaagaatag tgatagtatc catccctttt tctactggga atacaattac
      361 acgattcaaa gcttgataca caacatttcc cttcttataa accgctattg taaccgaatt
      421 gttttgaatc acctttttgg gatagaaact ggctcctttc cgcaacttct taaatgttgg
      481 tttcttttat actttctttg tagcaactac aaatttcttc aaatctctaa atgtttgcat
      541 aacaaccgcc tttctgactt ttactcagga tggtatttaa aaatcagcaa aataaggctt
      601 ctctccatct tttttatttc ctagctgctt taaagacact cttgctgttt tttaatctct
      661 atcaaggggg ctctgaatct attttgatga acttatctta ttaatttctc aaatgagctg
      721 tcagacacaa ctgtattttt tcaatctcat accggacaac tatgccagct taaaaaaatt
      781 tcctctggca aaacttcttt attgaaaaat gacaatgaaa aaagaccatt ctttttgttg
      841 aatgctcatt gtgtaaacat atttcctgcg actgctcttc gaaccattct cagtgcaatc
      901 cctaatttat ctattaaatc ttcttttagt tcctcatctt tcatattcaa aatttccttt
      961 gtatgttaca caagtttgtt ctctaaataa cgcgaattgt aaaattaagt tagaaaaata
     1021 aaaaggcatt tatggtacac tcaaattgta tttccgacga aagaaaacaa aggagtgtaa
     1081 ccataaatga cctacaaaca tcttaccata gacgaactga caatgataga atcatattat
     1141 cttcaacata ataagccggt tgaaatcgct aaccgaatgg gacgtgctat acaaactatt
     1201 tataatgtag tcaataagtt caagcaaggt aagactgctc ttgattattg gcaccagtat
     1261 aaagaaaata agaaaaaatg tggtagaaaa gtcattcaat tacctgctca tgaagtagat
     1321 tacattaaag agaaagtcac tcttggttgg acgcctgacg tc
//
LOCUS       DAZKNH010000203         1352 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000203 DAZKNH010000000
VERSION     DAZKNH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.208"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACG8MG_002733"
     CDS             <1..459
                     /locus_tag="ACG8MG_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376694.1"
                     /translation="AKYLKTHVEAIDGKEALVQTDGDQAIAGHKNFSGSVTINNKRVL
                     TTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYR
                     TPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            473..904
                     /locus_tag="ACG8MG_002734"
     CDS             473..904
                     /locus_tag="ACG8MG_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376695.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            906..1043
                     /locus_tag="ACG8MG_002735"
     CDS             906..1043
                     /locus_tag="ACG8MG_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0376696.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
     gene            1081..>1352
                     /locus_tag="ACG8MG_002736"
     CDS             1081..>1352
                     /locus_tag="ACG8MG_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376697.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          506 a          196 c          278 g          372 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 aacaagtaat ggttttcgtt ccgttgtgca ttactgaaga agaagcagat aaaatcatta
     1021 gcaaagaaga gagcgcatct taattgatgt gctttttatt ttgattcaag gagttgtcac
     1081 atgattaatt taggggaatg gggagcgata gcaggatcaa taaccgctat cgtttctttg
     1141 attttattag taataaaacc aattactgca tctttctcga agattactga gactctttca
     1201 aaagtaagtc acaatttaga tttgctgact aaagatttag aatcgagcaa atcagatcga
     1261 ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca tacagaaaaa
     1321 ttggtggaac acacgcaaca aattaaaact tt
//
LOCUS       DAZKNH010000204         1309 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000204 DAZKNH010000000
VERSION     DAZKNH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.210"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..165
                     /locus_tag="ACG8MG_002737"
     CDS             43..165
                     /locus_tag="ACG8MG_002737"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376698.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
     gene            138..269
                     /locus_tag="ACG8MG_002738"
     CDS             138..269
                     /locus_tag="ACG8MG_002738"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376699.1"
                     /translation="MQSAKACGIAFGLAKWGAKNIENFQSVDIIIDSPQEILDLLEK"
     gene            437..>1309
                     /locus_tag="ACG8MG_002739"
     CDS             437..>1309
                     /locus_tag="ACG8MG_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0376700.1"
                     /translation="MTYTHLTTDELVIIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNN"
BASE COUNT          462 a          214 c          242 g          391 t
ORIGIN      
        1 aatctatatt ttgatataca aattacagct tcagatacta agttgcataa accttttccg
       61 gaccccatac aaaaggcaat tgatgaattg ggcataaaaa ataagaaacg atttatattg
      121 gtgattcatt atatgacatg caaagtgcga aagcttgtgg gatagcattt ggtttagcta
      181 aatggggagc caaaaatata gaaaattttc aaagtgtaga tataattatt gattctcctc
      241 aagaaatact tgatttgtta gaaaaataga caaagagcgt gagataagga tagggtctca
      301 cgctctttgt ttttcgccat ttaaagattg aaatttttgc catcgccgat tgtaaaatta
      361 agctagacaa ctaaaaaagt catttgtgct acactcaaaa tagttccgac caaagaatta
      421 taaggagtga gtacaaatga cctataccca tcttacaacg gatgaacttg taataataga
      481 gtcttatttc aaaattaatc aatctgttgc taaaactgcc cattgcttga atcgttcaag
      541 acaaacgatc cataaagtat atctgttctt caagcaagga aaatcagcct tagaatatta
      601 tcaacagtat aagaaaaaca aatcaaactg tggtagacgt ccgcttgttt tacccgagga
      661 acaatcagaa tatattcaaa gaaaggttgt tcaaggatgg acacccgatg tgattgttgg
      721 tcgtgcagcg tttcctatta gttgttctgc tcgtaccatt tatcgtatgt tcaaaaaggg
      781 gctatttgat tcttctgact taccgatgaa aggcaagcgt aaaccgaatg gacatcaaga
      841 aagacgtgga aaacaaactt tccgccgctc tattcatgaa cgtgaaaagg attatagcca
      901 attctcaaat gagtttggtc accttgaagg tgacactatc gtaggtctga aacataaaag
      961 tgctgtaatt accttagttg aacgattatc aaaagttatc atcacattga aaccgtgtgg
     1021 tagacaagcg attgatattg aaaaaaaatt aaatcaatgg tttgaatctg taccgaaaaa
     1081 cctattcaaa tccatcactt ttgattgtgg aaaggaattt tcaaattgga aacagatcag
     1141 taatgtcaat gatattgcca tttatttcgc tgatccagga acgccgtctc aaagaggcct
     1201 aaacgagaat tctaacggat tgttacgtag agatggttta ttgaaatcta tggatttcaa
     1261 ttcagtagat gaatttttta ttcaatctgt cgcatctaaa cgaaataat
//
LOCUS       DAZKNH010000205         1298 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000205 DAZKNH010000000
VERSION     DAZKNH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.211"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..284)
                     /locus_tag="ACG8MG_002740"
                     /pseudo
     CDS             complement(111..284)
                     /locus_tag="ACG8MG_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACG8MG_002741"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACG8MG_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          461 a          216 c          177 g          444 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctaggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagtt
//
LOCUS       DAZKNH010000206         1275 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000206 DAZKNH010000000
VERSION     DAZKNH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.212"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..1203)
                     /locus_tag="ACG8MG_002742"
     CDS             complement(31..1203)
                     /locus_tag="ACG8MG_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFQ0376701.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          345 a          265 c          245 g          420 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc tttta
//
LOCUS       DAZKNH010000207         1260 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000207 DAZKNH010000000
VERSION     DAZKNH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.213"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8MG_002743"
     CDS             131..505
                     /locus_tag="ACG8MG_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0376702.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8MG_002744"
     CDS             complement(619..1248)
                     /locus_tag="ACG8MG_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0376703.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
BASE COUNT          425 a          179 c          226 g          430 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
//
LOCUS       DAZKNH010000208         1249 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000208 DAZKNH010000000
VERSION     DAZKNH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.214"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..740
                     /locus_tag="ACG8MG_002745"
     CDS             <1..740
                     /locus_tag="ACG8MG_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HFQ0376704.1"
                     /translation="NDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDL
                     TLLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWN
                     VTPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYI
                     SEDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTR
                     DMYKKYTGKTGCLDSTYAADIEERREQ"
     gene            737..1217
                     /locus_tag="ACG8MG_002746"
                     /pseudo
     CDS             737..1217
                     /locus_tag="ACG8MG_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          431 a          184 c          281 g          353 t
ORIGIN      
        1 ctaatgatga ctggcttaat caatttggga atataataat gatgcaaaag ccggaggata
       61 tggaattatt cccacctgaa gaaaagggat tttcctatct tatgctattt gatgattaca
      121 ataaaattga tcttacctta ttgcccttgg aagagttaga taattaccta aagggcgata
      181 aattaataaa ggttctaatt gataaagatt gtagaattaa aagggacata gttccgactg
      241 atatagatta tcatgtaaga aagccaagcg caagggagta tgatgattgc tgcaatgaat
      301 tttggaatgt aacaccttat gttattaaag gattgtgccg taaggaaatt ttatttgcta
      361 ttgatcattt taatcagatt gttcgccatg agctgctgag aatgatatca tggaaggtcg
      421 gcatcgaaac aggctttaaa ttaagtgtag gcaagaacta taagtttatt gaaaggtata
      481 tatccgagga tttgtgggag aaacttttgt ccacctaccg gatggattcc tatgaaaaca
      541 tatgggaagc attatttcta tgccatcaat tgttcagggc ggtatccggt gaggtggcgg
      601 aaaggcttca ttatgcctat ccggagtatg ataggaatat aacaaaatat accagggaca
      661 tgtataaaaa atacactggt aaaaccggct gcctggatag cacatatgcc gctgatatag
      721 aagagaggcg ggaacagtga ttacagaaat gaaagcaggg cacctgaaag atatcgataa
      781 acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa acgagaattg
      841 gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag acgaagagga
      901 tgaagaggat gaggaggcag attgccttga atatattgac aatactgata agataatata
      961 tcttttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc ataggcagcg
     1021 cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg
     1081 aaacccagga caataacctt atagcttgta aattctatca taattgtggt ttcaaaatcg
     1141 gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct
     1201 ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtctt
//
LOCUS       DAZKNH010000209         1249 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000209 DAZKNH010000000
VERSION     DAZKNH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.215"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1184
                     /locus_tag="ACG8MG_002747"
     CDS             <1..1184
                     /locus_tag="ACG8MG_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFQ0376705.1"
                     /translation="QAQFIKGRLDDSPSHCEHCGSIRIIRNGSYTTRTQMLKVKEKLT
                     ILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKKYFV
                     SDGTVTRILREATKHYQPRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGTTNQLFTIL
                     EDRRLYKLTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHI
                     NRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTLEPLKRHYHRLFKRPI
                     SQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRDTEGFQETLKTISSTLPQSFKKKFT
                     IFHRYQSGISNAFKVPHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFE
                     N"
BASE COUNT          382 a          272 c          244 g          351 t
ORIGIN      
        1 ttcaagcgca gtttattaaa gggagattgg atgactcacc ttcccattgt gaacactgtg
       61 gatcgattcg tattatccgc aacggttcct atacgacacg cactcagatg ttgaaagtca
      121 aagaaaagtt gactatttta gaattaaaac gtacccgttt tctctgccat gattgtggac
      181 ggaccttttc tgcgaaaacg gacttagttg atgaacatca tcaactaaca aaggaactga
      241 agcaggcgat tctcatggaa ctgtacgaaa atcaatcacg taaactaatc gctaagaagt
      301 acttcgtctc ggatgggacg gtgacacgga tcttgcgtga agcaacgaag cattaccagc
      361 cacgtatgaa cttccttccg acggtgttgt gtatggatga gttcaaatca atgaagtctg
      421 tttctggttc catgagtttt atttgtgttg atgggacaac caatcagcta tttacgatcc
      481 ttgaagatcg cagactttac aaactaactc aatacttcct gcgctttcct agaaaggccc
      541 gtttaaaggt caagtaccta gtgatggaca tgaatgccag ctattgtcag ttgcttaaaa
      601 cagtctttcc aaatgcggag atcgtaactg accgctttca tattgtccaa cacatcaatc
      661 gcagcttcaa tcaattacgt gtccagatca tgaatcgttt tcgaacctca cactctgaaa
      721 atcagaagaa gtaccgtcgg ctcaagcgat attggaaact cttactcaaa gattccacga
      781 cattggagcc actcaaacgg cattatcatc gactgtttaa acgaccaatt agtcaaacag
      841 aaatcgttga tgagctgtta agctacaacg aagaattgcg aactgcgtat catttttgtc
      901 aattattacg ctattatttc gttaaacgag atacggaagg ctttcaagaa acccttaaaa
      961 caatttcatc tacgttacct cagtcgttta aaaagaaatt cacgatcttc catcgctacc
     1021 aatcgggtat ttcaaatgcc tttaaggtgc cccactcaaa cggcgtcact gaggggctga
     1081 ataacaagat caagctgatt aaacgaattg cgtttggtta tcgcaacttt tataactttc
     1141 gggcacggat ctatttacaa caaggtctga tctttgaaaa ctaaaaaaat atgcggcgca
     1201 cagaacgcgc cgcatatcgg actaagtttt attcaaattt gtcttcgcc
//
LOCUS       DAZKNH010000210         1246 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000210 DAZKNH010000000
VERSION     DAZKNH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.216"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG8MG_002748"
     CDS             200..742
                     /locus_tag="ACG8MG_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376706.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="ACG8MG_002749"
     CDS             898..>1246
                     /locus_tag="ACG8MG_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFQ0376707.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAZKNH010000211         1218 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000211 DAZKNH010000000
VERSION     DAZKNH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.217"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..999
                     /locus_tag="ACG8MG_002750"
     CDS             46..999
                     /locus_tag="ACG8MG_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0376708.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            971..>1218
                     /gene="prgO"
                     /locus_tag="ACG8MG_002751"
     CDS             971..>1218
                     /gene="prgO"
                     /locus_tag="ACG8MG_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFQ0376709.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEER"
BASE COUNT          437 a          188 c          234 g          359 t
ORIGIN      
        1 atgaagtata ctaaatatat ttagtatagg aaattggagg gaactatgga aaacttacgg
       61 actgcattta aaacatcaga taaactagga aaagttgtaa tgcttacagg tagaaaaggc
      121 ggcattggta agactacaga caatgattta ttagcgattg tttctagtca actttttgaa
      181 aaagacgtgt tgctaattga ttatgaccaa caaagaaata ctacttccaa cattggatca
      241 acgtatcaaa ttactagctt tgatcgatcc atgtctgcag caatcaaaaa aggggactgg
      301 gtttcaggta ttactcaagt aagtccacat ttatatatta tggctggctc tcctggatct
      361 gaagaactta atgaatactt gtccgaaaaa tatcctgatc gtcgtaagag atcattggcc
      421 ttcattaaac ctttagaaga attgagaaaa aatttcgatt atatctttat cgactgccca
      481 ccatcaacag ataatgtggt tcgtgcattc ttaactgctg cagattatat tattgcaatg
      541 caagaattaa aacggtacgc aatggaagga acagaagatt ttattaataa agttttagtt
      601 ccgattgtca cgaattttga agagtctcat cttcaaatca ttggaatttt gcctgttctt
      661 ttctcagtgc gacgttcctc tcaacacgca aactatcaaa aaacaattga taagtatggt
      721 gaatcaaaca tttttaaaag cattgttaaa ggctcggatc ggttagaaat gtatggtgag
      781 aatggtatcc aattaaatga ttatgtagat cgaagatggt gggctatttt tgcagacatc
      841 tttacagaat tagaagaaag aattgaatac tatgaacaac atggtgatat tgaaggattt
      901 gattatacac ctaaatatgc tgactcaatg gccaatcata tcctacctct tggaaaggag
      961 attaaactaa atggcattat taccaacgaa caaagataag aagaaaattg gtgctgaaga
     1021 tatttcaatt aaagatgata ttaatgtcga gttaggggaa cgaaagtaca ctgcgaaaga
     1081 aagaaaacca gttcaagtag atcctcccgt actaaaaacc attcgggatc ttagttatgc
     1141 aaaagatatt cctatgtatg aaatagtcaa acttgcaatg gacgcctatg ttgatacttt
     1201 aaacgacgag gaacggat
//
LOCUS       DAZKNH010000212         1209 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000212 DAZKNH010000000
VERSION     DAZKNH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.218"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..316)
                     /locus_tag="ACG8MG_002752"
     CDS             complement(17..316)
                     /locus_tag="ACG8MG_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0376710.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(322..552)
                     /locus_tag="ACG8MG_002753"
     CDS             complement(322..552)
                     /locus_tag="ACG8MG_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376711.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(811..924)
                     /locus_tag="ACG8MG_002754"
                     /pseudo
     CDS             complement(811..924)
                     /locus_tag="ACG8MG_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          424 a          199 c          177 g          409 t
ORIGIN      
        1 ttttaggtag cctactttaa tctttgtatc tttctggatc aacgaaagta tactttatat
       61 agtcataacg ccgatgatcg cttcgagcgt ctagcacatc agtcacgata tcaaacaaaa
      121 aatatacgtc tttcttcatt ctagttttcg cagcagggat tttaaagtag ttcttattag
      181 aatagtagag attaattaat aagctatctt cgattgctaa aaagaaaact tctgaatccc
      241 acaccttata aaaatctttg acaaatctat tcgaaggatc aaatttaaac cataattgcg
      301 tctcattaaa aagcataacc attactccga tctattttta aacttagtct ctacctctaa
      361 tatatatcga gtttttattt tgccttcaga gaatactgtt tctttttttg cagttacagg
      421 ttgtttattt tcggaaaaag ctaatatagc taaaattgaa acatccatct ggaatttgtc
      481 tttttcgttg ctttgctcat aaaaatctgc atattcatca ctaatatttt ttctaataaa
      541 ttcttccatc ataaagatca cctcgaaaag agtatacgaa caaacgttct ttttgtaaag
      601 gtggaattta ttatactaaa taaataggtg aacaagtact tgtgccaaat tatgtgccaa
      661 aaaaaatcga atttaataaa acacaaacaa aaagaaatcc tattatgata acatttctta
      721 taataacaaa cacaacagaa gacgtgtaat agttagtcag gaacgtacaa ataacccctg
      781 tatcctttgc ggtacagggg gttattttgt ataagtagac attgtagttt ctaaactgct
      841 atgtcctaaa cgtttcgata cgctaagtat atttactcct tgataaagta aaatagatgc
      901 atgcgtatgt cttagtccat caataaacag tcgaagagaa aatagggaaa aattgattac
      961 taaagaaaaa cttactaggt cgacggtgta gttgattttc atacttcaat agattcgaat
     1021 taaacgattt cgattggctt ataccagtcc tatccaacga aagatatatc gttcttaact
     1081 ctagcggtat aacttgaagc tttacgtaaa gccaatggga atgaactgga gatgactttt
     1141 tattattttt agtaatagct gatgggttaa tacttacaac tttccttttc aaagtaccat
     1201 ataaaaaaa
//
LOCUS       DAZKNH010000213         1187 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000213 DAZKNH010000000
VERSION     DAZKNH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.219"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..148
                     /locus_tag="ACG8MG_002755"
     CDS             <1..148
                     /locus_tag="ACG8MG_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0376712.1"
                     /translation="QCGIIKMRDYLILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNY
                     LYRR"
     gene            153..1100
                     /locus_tag="ACG8MG_002756"
     CDS             153..1100
                     /locus_tag="ACG8MG_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0376713.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          386 a          172 c          253 g          376 t
ORIGIN      
        1 gcaatgtggg ataataaaaa tgagagatta tctaatttta gatattgata gtgaagaaga
       61 ttttaaatgg ttagaatttt tgtttcctaa atttttagaa gattcttcgg agcttcaaga
      121 tatctataat tatttatatc ggaggtaaaa ggatgagctg ttatcttatc accggtgggg
      181 ctggcttcat tggttctacc ttagccaatc atttaggaga agaacatgtg gtaatcgtcg
      241 tcgatgactt atcaatggga aaagtagaaa atcttgatat gacgaaaaat atcactttta
      301 ttgagggaga tgtcgctgag aaatccttga tggaagagat catgaaagct aataaatttg
      361 attatatctt tcatttagca gcagttgcta gtgtggcaga ttcagttgcc cgaccagtag
      421 aaacacatcg tgtgaatttt gaaagtgtat taatgatttt agaattgatc cgcaagtatc
      481 aacctgattt aaaacggatc gtgtttagtt cttcagcggc tgtttatggg gatgaaccga
      541 ctttaccaaa aaaagaagag tccgtgattc gcccattgac gccttatgcg gttgataaat
      601 tcgctgcaga acaatatgtg ctagattatt gtcatttata tgatgtaccc ggtagcgcag
      661 ttcgtttttt caatgtctat ggtccaaatc aaaatccgaa ttctccatac agtggtgtga
      721 tttctattct cgtagatcgc tataagaaac aaatcgtagg agaagcaact tcatttacat
      781 tatttggtga tggaagccag tctagagact ttgtttatat agatgatgta atccaagcat
      841 tacttttagt tgcgaatgag gaagctgcac tggggaaaca attcaatgtt ggaacgggta
      901 aagcaaccac acttttggaa ttgattcatg caatcaatga aattctcggt gtagaattga
      961 cacttgaata caaagaagag cgttcgggtg atatccatga ctctctagca gatattacta
     1021 agatccagtc aattggctat caaccgaaat atggtatttt aagtggaatg actaattatt
     1081 tgaaaactga aatcaattaa aacatactaa gataagatta gagggtaaat tcaggctctt
     1141 tgtcaataag gactgatgag ttttgtaaaa tcaaatctgg gatagaa
//
LOCUS       DAZKNH010000214         1170 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000214 DAZKNH010000000
VERSION     DAZKNH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.220"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..695
                     /locus_tag="ACG8MG_002757"
     CDS             288..695
                     /locus_tag="ACG8MG_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376714.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="ACG8MG_002758"
     CDS             709..1110
                     /locus_tag="ACG8MG_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376715.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
BASE COUNT          438 a          144 c          200 g          388 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata
//
LOCUS       DAZKNH010000215         1145 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000215 DAZKNH010000000
VERSION     DAZKNH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.222"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..1121
                     /locus_tag="ACG8MG_002759"
     CDS             102..1121
                     /locus_tag="ACG8MG_002759"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0376716.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT          398 a          183 c          258 g          306 t
ORIGIN      
        1 aaagaagtaa acaaacactt aaaaacaatg gaaattagtc ccaactttaa gaaatgtata
       61 ctataatgga aattatcaat tatttatttg gaggaatatc catgaaacaa aaaatcgcag
      121 ttcttggtcc tggatcttgg ggcactgctt tagcacaaac attggcagaa aacgggcatg
      181 atgtccgtat ttggggaaat gtacctgaac agattgatga aatcaataag tatcatacaa
      241 atcagcattt tttacctgat ttgacgatcc cagaatcgat tatcggatat aaagaattgg
      301 cagaagcagt ggataacgca gatgctattt tatttgttgt tccaacaaaa gcgattcgtt
      361 cagtcgcaca ggaacttatt ggtaaaatga atactaagcc aatcattata catgcaagta
      421 aaggactaga acaagatacg cataaacgaa tctcagaagt tttggctgaa gaaattccag
      481 aagaaaaaag acaggcaatt gttgtattat ctggtcctag ccatgctgag gaagtggcag
      541 tacatgatat cacaacaatt acagctgcaa gtattgatca aaaagcagca gcctatgtac
      601 aaaaactgtt catgaatgat tatttcagga tttatacaaa taatgatgtg attggcgtag
      661 aaactggtgc agcactgaaa aatatcattg caattggtgc cggcgctatc catggattag
      721 gattcggaga tgatgcaaaa gcagcaatca tgactcgagg attggcagaa atcagccgtc
      781 ttggtgtcgc gatgggtgcc aatcctctga catttatcgg tttgagtggt gtgggggact
      841 tgatcgttac ttgtacaagt gttcattctc gcaattggcg tgccggtaac ttgttaggac
      901 agggacataa gctggaagaa gttctagaaa acatgggaat ggtcgttgag ggtgtggcaa
      961 cgacaaaagc agcagtagag ctagctcaac agctaggtgt tgagatgcca attactcaaa
     1021 caatctataa tgttttgtat aatggagaag atatcaaaaa agcagcaaaa gaaatcatgt
     1081 tacgtgacgg aaaaatggag aatgaatttt ctcttcgtta aatagatgat caatagttaa
     1141 tcgtc
//
LOCUS       DAZKNH010000216         1140 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000216 DAZKNH010000000
VERSION     DAZKNH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.223"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..297
                     /locus_tag="ACG8MG_002760"
     CDS             <1..297
                     /locus_tag="ACG8MG_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0376717.1"
                     /translation="TYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIPEYYQMGNIDQK
                     VLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKISLRNWLEE"
     gene            complement(434..850)
                     /locus_tag="ACG8MG_002761"
     CDS             complement(434..850)
                     /locus_tag="ACG8MG_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376718.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(853..1137)
                     /locus_tag="ACG8MG_002762"
     CDS             complement(853..1137)
                     /locus_tag="ACG8MG_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376719.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
BASE COUNT          352 a          181 c          197 g          410 t
ORIGIN      
        1 acgtatgcaa aaactctatt ccgactgctt atgcagtacc gttctactgg ttattatgta
       61 gttcgtattg aagagtttag gagcttaatg gatatcccag aatactatca aatgggtaac
      121 attgatcaaa aagtattaaa tcctgcatta cgagaactaa aaaaatactt caatgatctt
      181 aaagtcacaa aaataaaagc aaaaaagggc aataaaatcg ccaaattaga atttactttt
      241 tcaagcctaa cgtcagattt gccaaagatt tctctgcgta actggttaga agaataaata
      301 aaaaagaact cgcccccctt acattaagca agtagacaag tcccttacaa ccgactaaga
      361 ttgctagtac attctagcat attgtatggg gcttctaaat aaaaaacaga taggaataac
      421 tcctatctgt tttttatcgt tttagaaaat ttgttgggtc gaaatttcta gaagtgactg
      481 tttgactggt ttctttttga tgattctttg gtcgatttcg atttgatgga tttagtttag
      541 cttcgatttt ttctttcact tcttgatata ctttagaatt tgtgccaata agaacgcctg
      601 tatcttttag atataaatca gcaaattctg cgatgatttc agtcatgatg gaaatttctt
      661 ttcccatagc atttagtttt gcctgattgc tggtagtggt cgtgttggta tcgggttcat
      721 aactcataca taaaaagcga atggcttccg agtagttatt gatatctgga tttttttcga
      781 tgatggaatc taaaacgtca acatgactag ggtacaaagt aacatgtttt tggacagaag
      841 tttttctcat ggttatatca ttcctccgat aagcatttca atgatttgat tatttgtgta
      901 gacatactcg cttaatattt gagtattttc tgtttggata gcaataaatt tatcgagcaa
      961 taaatcgaat tggtcttttt tgtaagtcgt aagttgacgg tcaaaatcac tttcgattaa
     1021 aacttttaga tatttatttc gactccattt ttctcctgtt ttattcgtga gttcttggca
     1081 acgtttgtct attgttgcaa caaactttgg atcaatattc cgaactaaaa tgtccataaa
//
LOCUS       DAZKNH010000217         1137 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000217 DAZKNH010000000
VERSION     DAZKNH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.224"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACG8MG_002763"
     CDS             <1..355
                     /locus_tag="ACG8MG_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376720.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="ACG8MG_002764"
                     /pseudo
     CDS             615..910
                     /locus_tag="ACG8MG_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..>1137
                     /locus_tag="ACG8MG_002765"
     CDS             985..>1137
                     /locus_tag="ACG8MG_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0376721.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          431 a          135 c          237 g          334 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      961 aaaaaagaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
     1021 gtcattattg tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag
     1081 gaattgttat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggta
//
LOCUS       DAZKNH010000218         1125 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000218 DAZKNH010000000
VERSION     DAZKNH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.225"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..669
                     /locus_tag="ACG8MG_002766"
     CDS             <1..669
                     /locus_tag="ACG8MG_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376722.1"
                     /translation="TSKNVQRVFKILNKNSIFKKKIDDYFVYCPELLLYDVKNIKKIP
                     KIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKK
                     ETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEK
                     TINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVE
                     YGII"
     gene            656..>1125
                     /locus_tag="ACG8MG_002767"
     CDS             656..>1125
                     /locus_tag="ACG8MG_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0376723.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSF"
BASE COUNT          398 a          143 c          156 g          428 t
ORIGIN      
        1 acaagtaaaa acgttcaaag agtttttaaa atattaaata aaaatagcat cttcaaaaaa
       61 aaaattgacg attattttgt ctattgtccg gaattgttac tttatgatgt aaaaaatata
      121 aaaaaaattc ccaaaataga tgaaaacagt aaattgatga aaaacataca atcaatcttt
      181 gggatagaag aagtttttgt cggagataaa tgtatctttt ttgatcaacc tttattatct
      241 gatggttatg gcatagatga aacagatatt ttcgctaaag taaatagtat attttctgga
      301 aaaaaagaaa ctttaattaa gcttcatcct agagcaagga caaatcgtta tggtgaaaaa
      361 aacatattga attccagttt accgtttgaa gtcttgctac ttgaaaatga cttctcttca
      421 acacttttga ttagtcctat atctacaatt tctttttcac catctatgat gtttgaaaag
      481 acgatcaact ctattatgtt ggctaaatta attttaaatg aatatacagc attatctgaa
      541 gataaaaaag aaacattgac aaatattgtt cttttctgtg aacattataa ccgtctatca
      601 gaagaaaaaa ttttactccc agaaaactgg gcagaattaa gaaagttggt agagtatggg
      661 attatttaac acactttcca agtcaaaatt agttaagtcc agtttctggt atactatagg
      721 gaattttttt attaagggaa tttcattcat aactattcct atttttgtaa atattactac
      781 tcaagcagaa tatggtttga ttaataattt cacttcattt gtatcaattt tttcgattct
      841 tgttgggcta agcctaaatt cggcaattaa taatgcaagt tttgactata aagatgacat
      901 aaatgaattt atgtcatcgg tactttctct agctacactt tcgttaattg tgttcttatt
      961 aatagggaac atatattttt tgttttcaaa tgcttttttt gatatgaacc aacttgtatt
     1021 taatttgatg ttattacaaa gttttgctag ttttgtaatt agttatatcc agtcttattt
     1081 tacgattaac tttaaccatt ttaaatattt gattttatct tttat
//
LOCUS       DAZKNH010000219         1112 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000219 DAZKNH010000000
VERSION     DAZKNH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.226"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..417
                     /locus_tag="ACG8MG_002768"
     CDS             154..417
                     /locus_tag="ACG8MG_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0376724.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..689
                     /locus_tag="ACG8MG_002769"
     CDS             417..689
                     /locus_tag="ACG8MG_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0376725.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            766..1095
                     /locus_tag="ACG8MG_002770"
     CDS             766..1095
                     /locus_tag="ACG8MG_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0376726.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          423 a          141 c          220 g          328 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac gcattacgag tcacagtaat tacttgtaaa tacattatct cgtggtataa
      721 tgtatttact aagtaattac atactgttag aaatgaggga tttaaatgat tacaaaaaca
      781 agaaaacaag gcaattcaat catgttgaca gtacctaaag attttaatgt tcctaatgga
      841 gtcgaagttg aagcaaagtt ggtagagaat ggtattcttt atgaatttgt tgaaccacaa
      901 aaagaatttt atgattttag cgaagatatt ttgtctgata taattgcaga aggctacgat
      961 aaagatgaga ttcttgtaga atttaagaat agaaaaaaca aaatgcattc ttcctttaga
     1021 gatattgctg aagatacact tactaattct aaagtaatga ctaaagagga gttggcaaca
     1081 gaaattggct tatgaaatcg ttccttcaaa ac
//
LOCUS       DAZKNH010000220         1107 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000220 DAZKNH010000000
VERSION     DAZKNH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.227"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..789)
                     /locus_tag="ACG8MG_002771"
     CDS             complement(55..789)
                     /locus_tag="ACG8MG_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0376727.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
BASE COUNT          393 a          218 c          199 g          297 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagt
//
LOCUS       DAZKNH010000221         1104 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000221 DAZKNH010000000
VERSION     DAZKNH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.228"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(548..>1104)
                     /gene="catA"
                     /locus_tag="ACG8MG_002772"
     CDS             complement(548..>1104)
                     /gene="catA"
                     /locus_tag="ACG8MG_002772"
                     /EC_number="2.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type A chloramphenicol O-acetyltransferase"
                     /protein_id="HFQ0376728.1"
                     /translation="EIDITLFKDMIKKKGYEIYPSLIYAIMEVVNKNKVFRTGINSEN
                     KLGYWDKLNPLYTVFNKQTEKFTNIWTESDKNFISFYNNYKNDLLEYKDKEEMFPKKP
                     IPENTIPISMIPWIDFSSFNLNIGNNSSFLLPIITIGKFYSENNKIYIPVALQLHHSV
                     CDGYHASLFMNEFQDIIHRVDDWI"
BASE COUNT          409 a          177 c          128 g          390 t
ORIGIN      
        1 taactcaaaa aatataatca ctcaaaattt aaaagggcgc acttatacat cattttaaaa
       61 aattgatgta acgtgctaag ttcaaaacaa agggcgcact tatacacgat tttcaatctt
      121 gtatatttct aacgaaaagc gtgcgccaaa aaaccccctt cgtcaatttt gacagggggc
      181 tttttgatgt aaaaatttct atcgaaattt aaaaattcgc ttcactcatg ttataaagac
      241 ttaaaataaa ataactcttt aaaatctttt gctagttgtt cttcaatatt ttttattcgg
      301 tgcatcttcc aagtaaagta taacacacta gacttattta ctacgtttca taagtcatta
      361 atgcgtgtgc tctgcgaggc tagtttttgt gcaagcacaa aaaatggact gaataaatca
      421 gtccataagt tcaaaaccaa attcaaaatc aaaaccacaa gcaaccaaaa aatgtggttg
      481 ttatacgttc ataaatttta tgatcactta cgtgtataaa attaaattca ctttcaaaat
      541 ctaaaaacta aatccaatca tctaccctat gaattatatc ttgaaattca ttcataaata
      601 gtgaagcatg gtaaccatca catacagaat gatgaagttg cagagcaact ggtatataaa
      661 ttttattatt ctcactataa aatttaccta tcgtaataat aggcaataaa aagctgctat
      721 tgttaccaat atttaaatta aatgaactaa aatcaatcca aggaatcatt gaaatcggta
      781 tggtgttttc aggtatcggt tttttaggaa acatttcttc tttatcttta tattcaagca
      841 agtcattttt ataattatta taaaaagaaa tgaagttttt atcagattca gtccaaatgt
      901 tagtaaattt ttcagtttgc ttattaaaaa ctgtatacaa aggatttaac ttatcccaat
      961 aacctaattt attctcacta ttaattcctg ttctaaacac tttattttta tttacaactt
     1021 ccataattgc ataaattaaa gagggataaa tttcatatcc tttctttttt atcatatctt
     1081 taaacaaagt aatatcaatt tctt
//
LOCUS       DAZKNH010000222         1073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000222 DAZKNH010000000
VERSION     DAZKNH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.230"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1073
                     /locus_tag="ACG8MG_002773"
     rRNA            <1..>1073
                     /locus_tag="ACG8MG_002773"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAZKNH010000223         1066 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000223 DAZKNH010000000
VERSION     DAZKNH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.231"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="ACG8MG_002774"
     CDS             complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="ACG8MG_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0376729.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          311 a          171 c          187 g          397 t
ORIGIN      
        1 gggtcccgag cgcttagtgg gaatttgtac cccttatcga tacaaattcc ccgtaggcgc
       61 tagggacctc tttagcttct tggaagctgt cagtagtata tctaataatt tatctccatt
      121 ccctttagta acgtgtaact ttccaaattt aaaaaagcga ctcatagaat tatttcctcc
      181 cgttaaataa tagataacta ttaaaaatag acaatacttg ctcataagta atggtactta
      241 aattgtttac tttggcgtgt ttcattgctt gatgaaactg atttttagta aacagttgac
      301 gatattctcg attgacccat tttgaaacaa agtacgtata tagcttccaa tatttatctg
      361 gaacatctgt ggtatggcgg gtaagtttta ttaagacact gtttactttt ggtttaggat
      421 gaaagcattc cgctggcagc ttaagcaatt gctgaatcga gacttgagtg tgcaagagca
      481 accctagtgt tcggtgaata tccaaggtac gcttgtagaa tccttcttca acaatcagat
      541 agatgtcaga cgcacggctt tcaaaaacca cttttttaat aatttgtgtg cttaaatggt
      601 aaggaatatt cccaacaatt ttatacctct gtttgttagg gaattgaaac tgtagaatat
      661 cttggtgaat taaagtgaca cgagtattca gttttaattt ttctgacgat aagttgaata
      721 gatgactgtc taattcaata gacgttacct gtttacttat tttagccagt ttcgtcgtta
      781 aatgcccttt acctgttcca atttcgtaaa cggtatcggt ttcttttaaa ttcaattgtt
      841 ttattatttg gttgagtact ttttcactcg ttaaaaagtt ttgagaatat tttatatttt
      901 tgttcatgta atctctcctg aagtgattac atctataaac aaatacagaa gttaaacgat
      961 ttgtttgtaa ttttagttat ctgtttaaaa agtcataaga ttagtcactg gtaggaatta
     1021 atctaacgta tttatttatc tgcgtaatca ctgtttttag tctgtt
//
LOCUS       DAZKNH010000224         1062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000224 DAZKNH010000000
VERSION     DAZKNH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.232"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>1062)
                     /locus_tag="ACG8MG_002775"
     CDS             complement(37..>1062)
                     /locus_tag="ACG8MG_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFQ0376730.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DAZKNH010000225         1003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000225 DAZKNH010000000
VERSION     DAZKNH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.233"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1003
                     /locus_tag="ACG8MG_002776"
                     /pseudo
     CDS             370..1003
                     /locus_tag="ACG8MG_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          295 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta cct
//
LOCUS       DAZKNH010000226          996 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000226 DAZKNH010000000
VERSION     DAZKNH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.234"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACG8MG_002777"
     CDS             complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACG8MG_002777"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFQ0376731.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          262 a          220 c          156 g          358 t
ORIGIN      
        1 ctttttcaca acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc
       61 atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa
      121 tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag
      181 cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga
      241 ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg
      301 atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac
      361 aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg
      421 tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc
      481 tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc
      541 atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt
      601 tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc
      661 tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat
      721 gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc
      781 gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa
      841 atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat
      901 gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt
      961 tgaaaaaaca tttgcaataa atcagaaaat agtggt
//
LOCUS       DAZKNH010000227          966 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000227 DAZKNH010000000
VERSION     DAZKNH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.235"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..461)
                     /locus_tag="ACG8MG_002778"
     CDS             complement(<1..461)
                     /locus_tag="ACG8MG_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0376732.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
     gene            complement(745..>966)
                     /locus_tag="ACG8MG_002779"
     CDS             complement(745..>966)
                     /locus_tag="ACG8MG_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0376733.1"
                     /translation="LKEKFLNLIYDEIAIDPYKGEQKTGDLSGIWSSGFKYAGTTYRV
                     AYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          312 a          184 c          128 g          342 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaga
      541 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
      601 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
      661 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa agaagccgtc
      721 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
      781 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
      841 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
      901 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
      961 tttcaa
//
LOCUS       DAZKNH010000228          958 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000228 DAZKNH010000000
VERSION     DAZKNH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.236"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..>958
                     /gene="bsh"
                     /locus_tag="ACG8MG_002780"
     CDS             4..>958
                     /gene="bsh"
                     /locus_tag="ACG8MG_002780"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0376734.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQ"
BASE COUNT          333 a          128 c          172 g          325 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaac
//
LOCUS       DAZKNH010000229          947 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000229 DAZKNH010000000
VERSION     DAZKNH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.237"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..878
                     /locus_tag="ACG8MG_002781"
     CDS             <1..878
                     /locus_tag="ACG8MG_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0376735.1"
                     /translation="SLNQPLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPRKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKRDRRSKHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
BASE COUNT          298 a          210 c          191 g          248 t
ORIGIN      
        1 tttctttgaa ccagccgtta ccagaagtgg tgtgtttcga tgaattcaaa tcggtgaaaa
       61 acgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaacta aatcctttac gcgactattt cttgcgctac cctagaaaag
      181 tccgtgaaca agtacgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgctt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacatcg
      301 gtcgggcctt taccaaccac cgaattcaag taatgaagac ctttaaaaaa agggatcgtc
      361 ggtcgaagca cttaaagaaa tactggagac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gactccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgctat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatat cgaacagaaa
      661 tcaaacgagc gttgcgagtt ccctattcca acggccctat agaatgtttg aacaaccata
      721 tcaaagtact aaaacgaatt gcctatggct ttagaaactt tcaaaactac caagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa cactacccaa ctgactaaag
      841 ctagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
      901 ctattgaatt tcggctctat actttttgat gttggtgaac ttcacca
//
LOCUS       DAZKNH010000230          944 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000230 DAZKNH010000000
VERSION     DAZKNH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.238"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(9..81)
                     /locus_tag="ACG8MG_002782"
     tRNA            complement(9..81)
                     /locus_tag="ACG8MG_002782"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46..48),aa:His,seq:gtg)
     gene            complement(84..157)
                     /locus_tag="ACG8MG_002783"
     tRNA            complement(84..157)
                     /locus_tag="ACG8MG_002783"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(123..125),aa:Trp,seq:cca)
     gene            complement(164..244)
                     /locus_tag="ACG8MG_002784"
     tRNA            complement(164..244)
                     /locus_tag="ACG8MG_002784"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(208..210),aa:Tyr,seq:gta)
     gene            complement(246..321)
                     /locus_tag="ACG8MG_002785"
     tRNA            complement(246..321)
                     /locus_tag="ACG8MG_002785"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(286..288),aa:Phe,seq:gaa)
     gene            complement(357..429)
                     /locus_tag="ACG8MG_002786"
     tRNA            complement(357..429)
                     /locus_tag="ACG8MG_002786"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(394..396),aa:Asp,seq:gtc)
     gene            complement(439..511)
                     /locus_tag="ACG8MG_002787"
     tRNA            complement(439..511)
                     /locus_tag="ACG8MG_002787"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(476..478),aa:Val,seq:tac)
     gene            complement(527..598)
                     /locus_tag="ACG8MG_002788"
     tRNA            complement(527..598)
                     /locus_tag="ACG8MG_002788"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(563..565),aa:Glu,seq:ttc)
     gene            complement(613..702)
                     /locus_tag="ACG8MG_002789"
     tRNA            complement(613..702)
                     /locus_tag="ACG8MG_002789"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(666..668),aa:Ser,seq:gga)
     gene            complement(742..815)
                     /locus_tag="ACG8MG_002790"
     tRNA            complement(742..815)
                     /locus_tag="ACG8MG_002790"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(779..781),aa:Asn,seq:gtt)
     gene            complement(825..940)
                     /gene="rrf"
                     /locus_tag="ACG8MG_002791"
     rRNA            complement(825..940)
                     /gene="rrf"
                     /locus_tag="ACG8MG_002791"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          257 a          263 c          215 g          209 t
ORIGIN      
        1 ataaaaaggg cgaatgatgg gaatcgaacc cacgaatgcc ggagccacaa tccggtgcgt
       61 taaccacttc gccacattcg ccatggcagg ggcagcagga attgaaccca cactaacggt
      121 tttggagacc gctgttctac ctttaaacta tgcccctata aaatggagga gagtggattc
      181 gaaccactga accctaagga acggatttac agtccgtcgc gtttagccac ttcgctactc
      241 ctccatggtg gcgcgggaca gaatcgaact gccgacacac ggagcttcaa tccgttgctc
      301 taccaactga gctaccgcgc catattttaa aattattaat tgaataacaa gaacgacggt
      361 tccgacggga atcgaacccg cgatctcctg cgtgacaggc aggcatgtta acccctacac
      421 cacggaacca gttcattatg gaggttaacg ggatcgaacc gctgaccctc tgcttgtaag
      481 gcagatgctc tcccagctga gctaaacctc caacattcaa ttaaaatgac ccgtacggga
      541 ttcgaacccg tgttaccgcc gtgaaagggc ggtgtcttaa ccgcttgacc aacgggccag
      601 gtaaatttgc tacggagagt aagggattcg aacccttgag acagtttggc accatctaca
      661 tgatttccaa tcatgctcct tcggcctctc ggacaactct ccagagtgaa agaagctcaa
      721 tactctttct caaaatgaga actccggcag taggactcga acctacgaca tcatgattaa
      781 cagtcatgcg ctactaccaa ctgagctatg ccggaataac atcagcgtgg cgacgtccta
      841 ctctcacaag gggcaacccc tcactacaat cggcgctaag aagcttaact tctgtgttcg
      901 gcatggttac aggtgtatcc ttctcgctat cgccaccaca ctgt
//
LOCUS       DAZKNH010000231          942 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000231 DAZKNH010000000
VERSION     DAZKNH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.239"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..>942
                     /locus_tag="ACG8MG_002792"
     CDS             4..>942
                     /locus_tag="ACG8MG_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376736.1"
                     /translation="MCNIKKHEVVYEFEKIFSVKIFIVYAFVSLLYLFPAFKNMEIFG
                     FYDTAFHINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIG
                     YNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLA
                     MSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRK
                     KKIEIFLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFL
                     IGSIDSELLSFGIGLTLLICLIILTFNFKKLGILSKI"
BASE COUNT          307 a          122 c          133 g          380 t
ORIGIN      
        1 actatatgta atataaaaaa acatgaggtg gtatatgaat ttgaaaaaat tttttctgta
       61 aaaatattta tagtttatgc atttgttagt ttattatatt tatttccagc atttaaaaac
      121 atggaaatat ttggctttta tgatacggca tttcacatta atcgtgcgtt aagtttagaa
      181 tctattttta gctcccctat taattttgaa acgtttagat catatggcat gcaagtaaat
      241 aatttttacc catggttaac tctatatcct ttatttttat tgatcaagtt tactaattta
      301 gcaataggct ataacctttt tctttatatt gttacgctca ttactctatt tatatgtcat
      361 tatgtaatgt atgaaattac caaaaaacat gttactagct cctttttcgc tattatatat
      421 acaacatctt cttttaggag tgtagaaata tttcttcgag gggcaatggg ggaattactc
      481 gcaatgtcaa tacttcctct tattttactc gggtttataa agctttacga ctcaaaaaaa
      541 gaatcttggg ttatgttggc gattagcatg actttattaa tttacactca tgttctttct
      601 gtagctatga ctatggtaat gattataatt gctttaatta ttcaatttta tcggaaaaag
      661 aaaatagaaa tcttcttaat tattaaatta ataaaagctg ctatagttac tttacttctt
      721 tcactagcgt ttataggtcc tatgattgag cagacattgt atcaagatct gaatagacct
      781 tatgtggata tattacaaaa aagagcaatt tatctgggta aagagttttt gattggaagt
      841 atagattcag aattactatc atttggtatt ggcttaactt tgcttatttg tttaattata
      901 ctaacattta actttaaaaa attaggaatc ttatctaaaa tc
//
LOCUS       DAZKNH010000232          894 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000232 DAZKNH010000000
VERSION     DAZKNH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.240"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            253..876
                     /locus_tag="ACG8MG_002793"
     CDS             253..876
                     /locus_tag="ACG8MG_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0376737.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          329 a          129 c          167 g          269 t
ORIGIN      
        1 aaactaatgt ccttattttt gtgttcacat ttctgttacc taagaaatac ttaaaggagg
       61 tgatgtagcc agcaacgact acaacttgaa cccgctatct acagtttatt tgttgtttct
      121 tttgggtata ctctaatggt acaaatccat atttgcttaa agcgttgatt tttcagcggt
      181 ttttctttat tgcaaagtgt accctaagac ctgtatacta atagtaaata gattggatag
      241 gaatgatggc aaatgtattt agcttattta agagtttctt ccgacgatca aagtttagct
      301 cggcaacata agctaattga aaattggtca gtcaaaaata atattgctga agacgattta
      361 aaaattttcg aggaaaaagt ctctggaaaa aatattgaaa acagaccaga gctgcaagag
      421 cttatttctt tcatacgtga acgagatacg attattattc ctagtcttga tcgattagca
      481 agaaatagta aagatacaaa agacttactt caattgtttc gtagtaaagg tgcttctatt
      541 gaaatattag acctaccgtc gtttaatggg gtaactgacc ctgcattaag agacttgcta
      601 acaaatttag tcatagaagt gtttagctat gttgctgaaa acgaacggaa aaaaatcaaa
      661 gagcgtcaaa aacaaggcat tgaaattgca aagaaaaatg gtcgttatac tggtaaaaag
      721 attcaatacc atgccaatgc aacaggaaaa aacaaattga tttacgatga aattgttgat
      781 ggtctgaaga aaaaagaaac tatttctaaa atagcaaaga acgcaggagt tacaagaaaa
      841 accgtttatc gagtaaaaag agatttagga atgtaatatg ttccagttag aaag
//
LOCUS       DAZKNH010000233          890 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000233 DAZKNH010000000
VERSION     DAZKNH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.241"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACG8MG_002794"
     CDS             <1..888
                     /locus_tag="ACG8MG_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFQ0376738.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAZKNH010000234          811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000234 DAZKNH010000000
VERSION     DAZKNH010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.244"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACG8MG_002795"
     CDS             <1..672
                     /locus_tag="ACG8MG_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0376739.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAZKNH010000235          791 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000235 DAZKNH010000000
VERSION     DAZKNH010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.245"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..392)
                     /locus_tag="ACG8MG_002796"
     CDS             complement(243..392)
                     /locus_tag="ACG8MG_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376740.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT          286 a          121 c          112 g          272 t
ORIGIN      
        1 tatattaaat attatacaga taataacgga atattatcat cgaaacagct aacaaaattg
       61 tcataaaaaa cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa
      121 gagagacaat ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt
      181 tacaaacgtc ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact
      241 ccttatctag attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg
      301 ttattttcgt tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg
      361 cggtattgca tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt
      421 agttcttgaa agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt
      481 ttgatgcaat taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc
      541 tattttcttt taatccgata agtagcccaa tcatcaaggt tacaaatact attgcttgaa
      601 tataaatcat atgctcacct ctttataaat aacttagcaa tataaagaaa aaaacgcacg
      661 aacaacatag gaatataaac aaacgttttt gtcacaacgt ctaagatcgt gttaaaaaat
      721 agttatctaa aaagcagaaa gatactcaaa ataaaaaata aaaaaatgga taaaaacgtt
      781 ttcaaataca a
//
LOCUS       DAZKNH010000236          775 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000236 DAZKNH010000000
VERSION     DAZKNH010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.246"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..259
                     /locus_tag="ACG8MG_002797"
     CDS             <1..259
                     /locus_tag="ACG8MG_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0376741.1"
                     /translation="EIHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMP
                     ESANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            260..>775
                     /locus_tag="ACG8MG_002798"
     CDS             260..>775
                     /locus_tag="ACG8MG_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0376742.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSR"
BASE COUNT          301 a           87 c          138 g          249 t
ORIGIN      
        1 agaaattcat ttagattatt ttggaagagt tcctacaaga aaatttcaat tgtttacttt
       61 agatgaatgt atcaatgttg acttattaaa taataaaata agttttgaag aaaacattgt
      121 taaagaaatg cctgaatctg ctaatgatat gtatgagaag gagttgaaat atttctttga
      181 aaacgtagta acaggcaaag aaaactggaa tgatttatat catgcaaacg aagtattgaa
      241 aatagctgag gagaaataaa tggaaatttt atttactatt tgtggtagag ctgggtctaa
      301 ggggtttaaa aataaaaatt tgaaatattt ggcaggaaag cccctagtcc atttcgcttt
      361 agcaatgata gatatctatc aacaaaaaca tcctgaacat aattgcacga cttgtttgaa
      421 tacggacagt aatgagttag tagaattgac gactaagtta aatccgtctg tgaaaattgt
      481 agaacgtaaa cctgaattag ctacagatac tgtagggaaa attgaagtga tacgtgattc
      541 attcttacaa ctagaaagag aagataatat atttgattta attatagatt tagatttgac
      601 ttcaccttta cgtgaagttt ctaatcttga agaattaata gatgaacatc taaaaaattt
      661 agataaagac gtctactttt cagttgtttc tgcaagacgt aatccctatt ttaatatggt
      721 aaaaagaatt gaaaatgaag ttactatagt taataaatca agatacacat ctagg
//
LOCUS       DAZKNH010000237          774 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000237 DAZKNH010000000
VERSION     DAZKNH010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.247"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            350..748
                     /gene="spxA"
                     /locus_tag="ACG8MG_002799"
     CDS             350..748
                     /gene="spxA"
                     /locus_tag="ACG8MG_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFQ0376743.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          289 a          107 c          137 g          241 t
ORIGIN      
        1 tttaaaatga cagaaaacat tactattaaa gaaataaaaa acataaaaaa tcttaagaaa
       61 tcggtttcat ttagcttttg agataaaata atagtacagt tttatacgct gtttcatatg
      121 tttagaaaat ttgaattaga tttgaaaact ctaattttta aatgataaaa aaagcgagaa
      181 tgctggtttt actgtatatt aaccttagat aactttcatt tttttaaaaa aattatgaaa
      241 aaaaggtagc tatttaaaag ttgattatgt ataatagaaa cagatgggag attattatga
      301 tcttctgtta caaaaaagtg attcttatca cgaaaaggag tggtgcacgt tgttaacatt
      361 atatacttcc ccaagttgta cttcctgcag aaaggctcgt gcgtggttgc aagaacatca
      421 gattccattt gttgaacgta atattttttc agaaccattg aacagttctg aattaaaagc
      481 gattttacaa atgacagaag acggaacaga agaaatcatc tcgacacgtt ccaaagtatt
      541 ccaaaaactg aacatggatt tagatgatct gccgttacaa gaattattgg aacttgtgca
      601 aaacaatcct ggattgcttc gccgtccaat catgattgat gataaacgtc tgcaagttgg
      661 ttttaatgaa gatgaaattc gtcggttcct accgcgcgat gtacgacaat tagaattacg
      721 tcaagcacaa ttgatggctg gattatagaa ggataatacg aaacttggaa aaga
//
LOCUS       DAZKNH010000238          753 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000238 DAZKNH010000000
VERSION     DAZKNH010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.248"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..670
                     /locus_tag="ACG8MG_002800"
     CDS             32..670
                     /locus_tag="ACG8MG_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFQ0376744.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          234 a          122 c          124 g          273 t
ORIGIN      
        1 cttcaaaaac tgaatagtag gagttattat catgaataac attttaggaa aaaaaataga
       61 acttaaaaaa gggatattgg gcactattgt cgttgtactc ggtttgttag ggttgctatt
      121 tgcgccatcc attattagca ttttttcttt aactatcact ggatttatag catttacact
      181 tgtttttggt aaaaaagata cgaaaaaaat gttctcgaaa cctgtcgctc ctgtcaaaaa
      241 tatagccaaa tattttttta tcaatgtagt aatcagtgcc gcagtctcgg tatttttgca
      301 acaaattttg aaatggggtt taacaggcaa tccaataaat gaggctttca gcccgttatt
      361 atttattatt ttgcctatca tgatacttgg agaagaattg ttctcagtgt attttctcgc
      421 tattttttct agtaagttca gcattcctgt cgctagtttc ttgtcagcaa tcatctttgg
      481 atttatccat tacagtactt atgataatgg aaatatttta cacactgtag cacacatcct
      541 tttgatacaa ggggtggcaa gattgttatt caaccaagct gccattaaaa gcaactcgat
      601 cattacaagt tgggcagttc atgtgatatt tgatttcaca gctatattgc tagtcgtttt
      661 attctcctaa atttttatag aaataataca tgctctattg ctaagaaagc atactttatt
      721 tcctttaaac aaaaagacag atgtatcaat gga
//
LOCUS       DAZKNH010000239          736 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000239 DAZKNH010000000
VERSION     DAZKNH010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.249"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>736)
                     /locus_tag="ACG8MG_002801"
     CDS             complement(109..>736)
                     /locus_tag="ACG8MG_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFQ0376745.1"
                     /translation="TSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTY
                     VRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGK
                     EFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLE
                     LFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          207 a          167 c          139 g          223 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtc
//
LOCUS       DAZKNH010000240          729 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000240 DAZKNH010000000
VERSION     DAZKNH010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.250"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..>729
                     /locus_tag="ACG8MG_002802"
     CDS             90..>729
                     /locus_tag="ACG8MG_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0376746.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHTSYLQHSKIT"
BASE COUNT          267 a           82 c          143 g          237 t
ORIGIN      
        1 gatcagagtc tatttttttc agagaattta agaaaatcgt tgatgccgtc attgactaat
       61 tgagaaaacg tgagtgaaag gatagaagga tgaattcgag tgatctaatt agtgtaatca
      121 tacctgtata taatgttgaa aagtatgtag gaagttgttt ggaatcagtg tgtaatcaaa
      181 catataagaa cctagatatt ataattgtgg atgatggatc taaggattca tctaataaaa
      241 tatgtgaaga ctatgcatta aaggattcaa ggattcgtat tattcataaa aataatgctg
      301 gcttgggatt ggcaagaaat acaggacttg aacatgtaaa aggaaaatat gttgctttta
      361 ttgattctga tgattttgtt gcagctaatt atattgaagc aatgttaaat ggtataaaaa
      421 ggagtaatgc agacacatgt tatgctggat attatgaata ttatgatgat aataatatta
      481 tttcaaagcc agcaagatat gatagtacga tatttacaaa taacgctata attgaaaaag
      541 tgttgcttgg aatgattggc actgagccga gttataaagt cgattttata ttgccaatgt
      601 cagtttggca tgcgctgtat tcatctgaga taattcaaca gcacaatatc aagtttccgt
      661 cagaaaggga ttatatatct gaagatatca tattccatac ttcatatttg caacattcaa
      721 aaataacaa
//
LOCUS       DAZKNH010000241          725 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000241 DAZKNH010000000
VERSION     DAZKNH010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.251"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>725
                     /locus_tag="ACG8MG_002803"
     CDS             <1..>725
                     /locus_tag="ACG8MG_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFQ0376747.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGG"
BASE COUNT          226 a          112 c          179 g          208 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggaca
//
LOCUS       DAZKNH010000242          719 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000242 DAZKNH010000000
VERSION     DAZKNH010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.252"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(334..>719)
                     /locus_tag="ACG8MG_002804"
     CDS             complement(334..>719)
                     /locus_tag="ACG8MG_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HFQ0376748.1"
                     /translation="QLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLFI
                     LNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVES
                     NYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT          247 a          101 c          125 g          246 t
ORIGIN      
        1 tgattcccct tgaaaatgaa ttgaggtaaa cgagaagaat tcaataaact ggatgataaa
       61 aatataatat gactataaat tggttacagc ttcaacctat aagttgtaat aaattagcct
      121 aaaagtaatt tattaatgat gaggtgaatt tgattggtac aacaagatat atcacttaga
      181 gagggagaag cagcactaca aactactttt caataagtag gaattataga aattttactc
      241 aaaaaatctt cagtaaacaa tttggctaaa taagattcag ttgttatacg agaaggttac
      301 ggttcttctt aactaagctt agtatgtttt tattcaaggc atgattttct ctatgtgatc
      361 attcagtttc tgtaagagtt ctaataattc ttttttggag tacttgtaat tgctttctac
      421 ccaccgattg agaacggata gacaaccttg gacgtggtac gtagtaaaga atctcatttc
      481 ttctgtgata atcgttttct tgtcttcata tagccatatg ttcagatagt tttcttcaat
      541 aacttgttta actttcttct gaaacgaaat atcaccatgg ttgaggataa ataagcgata
      601 taaaaaagta tttgcgtaaa catagtcaat caagcgagtg tatagttcct catagctatg
      661 ggctggatca taagagagaa tttcatttag atcccgaaaa aagttgcttt ctaattgat
//
LOCUS       DAZKNH010000243          717 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000243 DAZKNH010000000
VERSION     DAZKNH010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.253"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..608
                     /locus_tag="ACG8MG_002805"
     CDS             <1..608
                     /locus_tag="ACG8MG_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0376749.1"
                     /translation="IGTMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGT
                     GVGGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRL
                     GKKGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFA
                     KLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          201 a          142 c          182 g          192 t
ORIGIN      
        1 tcatcggtac catgaaaaaa gaatttccga tcccgatttc ttggacaaca gatgtcaatg
       61 cggctgccta tggtgagtat gtcttcggca gtggcaaagg attgagcagt gtcgtttact
      121 atacgatcgg tacgggtgtt ggcggcggtg ccttacaaga agggcgcttt atcgaagggt
      181 ttagtcatcc agaaatgggg catatgctcg tcgtccctca tccaaaagat gactttgctg
      241 gcagctgtcc atttcatggc aactgtttag aagggatggc cgctggaccc gccatcgaaa
      301 aacgcttagg gaaaaaagga caagaggttg cagaagatga tccttattgg gaaatcgaag
      361 cctcctatat tgctcaatgt gcctacaata caaccttgat gttttctcca gatgtgatca
      421 tttttggcgg tggtgtgatg aaacaacgcc atatgctgca aaacgtccac gacgcatttg
      481 caaaattggt caatgggtat gtcgaaacac ctgagttaag caaatatatc gtgaccccag
      541 cattagaaga caacgctgga acgctcggtt gtttggcatt ggctcgagaa gcggtcttac
      601 attcataaac gaaaaaaggc tcttcgacat ttagtgttgg tagagaaaga tacaactgaa
      661 aatttagtca taaccagtta ggcagagtgc ttaactggtt tttgttttac ttggaag
//
LOCUS       DAZKNH010000244          711 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000244 DAZKNH010000000
VERSION     DAZKNH010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.254"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..239
                     /locus_tag="ACG8MG_002806"
     CDS             <1..239
                     /locus_tag="ACG8MG_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HFQ0376750.1"
                     /translation="GNNIVLKKSFYGCSNYPECKFTLAEHFRKKKLTKTNVKELLEGK
                     ETLVKGIKNKEKKPYNAVVKIGEKGYIDFISFSK"
     gene            373..612
                     /locus_tag="ACG8MG_002807"
     CDS             373..612
                     /locus_tag="ACG8MG_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0376751.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
BASE COUNT          301 a           82 c          135 g          193 t
ORIGIN      
        1 gtggcaacaa tattgtatta aaaaaatcgt tttatggttg ttcaaattat cctgaatgta
       61 agtttacttt agctgaacat tttagaaaga aaaaactaac caaaacgaat gtaaaagaat
      121 tactggaggg aaaagaaacc ctggtaaaag gaatcaaaaa caaagagaaa aagccctaca
      181 atgccgttgt aaaaattggg gaaaagggat atattgattt tatatctttc tcaaaataaa
      241 cataaaagcc ctttaaagag ggcttttata tattaatcac aaatcactta tcacaaatca
      301 cttatcacaa atcacaagtg atttgtgatt gttgatgata aaataagaat aagaagaaat
      361 agaaagaagt gagtgattgt gggaaattta ggcgcacaaa aagaaaaacg aaatgataca
      421 ccaatcagtg caaaaaaaga tataatggga gataagacgg ttcgtgttcg tgctgacttg
      481 caccatatca taaaaatcga aacagcaaag aatggcggaa acgtaaaaga agttatggaa
      541 ataagactta gaagcaaact taagagtgtg ttgatagtgc attatcttaa aattttgtat
      601 aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc
      661 atgttggtat tccaaatgcg taatgtagat aaaacatcta ctgttttgaa a
//
LOCUS       DAZKNH010000245          691 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000245 DAZKNH010000000
VERSION     DAZKNH010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.255"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>691
                     /locus_tag="ACG8MG_002808"
     CDS             82..>691
                     /locus_tag="ACG8MG_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFQ0376752.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKL"
BASE COUNT          230 a          120 c          136 g          205 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg c
//
LOCUS       DAZKNH010000246          686 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000246 DAZKNH010000000
VERSION     DAZKNH010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.256"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            108..>686
                     /locus_tag="ACG8MG_002809"
     CDS             108..>686
                     /locus_tag="ACG8MG_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0376753.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLN"
BASE COUNT          239 a           99 c          111 g          237 t
ORIGIN      
        1 gcgacactat cgttatcgaa aaaggcgaaa acctatctca ttttctgcta atgtaagttg
       61 gtttagcttt tgttaatatg actttatgaa aatggaggta attaaatatg atttttccta
      121 aagttaatga agcaagcttg aataaaggat ccttttttca tatctatgaa aacgaagatt
      181 ttgaactgta taatatcctt tcacaaaaag aggtcatcga aaaccaaata atctgccatc
      241 caattattta caaatctatt ttcaagtatg cattttttgc ttccaatgat tttcaaagaa
      301 tggagattac cgactgtata ttcgaaaact gtgatttttc aggctgtata ttgacaaaaa
      361 gcatgttgca tcgcgtgaaa tttgtcaatt gtaaattaac aggtactaaa ttgatggaaa
      421 gttatattgg gaacacgtta ttcgagaatt gtaaaatgga ctatgtaaat ttatctggtt
      481 caaacataaa agaaagtacc tttgagcact ctgtcttatc aagcgcagat tttgtagact
      541 gttccctcac taaaataatg ttttctacta atgatttaga aaatgctagc ttttttgata
      601 ctgatttaaa aaatatcgat ttaagtagca actcctttga aatgattgaa gtacgctcgg
      661 aacatctaaa aaattgtacg cttaat
//
LOCUS       DAZKNH010000247          683 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000247 DAZKNH010000000
VERSION     DAZKNH010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.257"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACG8MG_002810"
     CDS             132..344
                     /locus_tag="ACG8MG_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376754.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>683
                     /locus_tag="ACG8MG_002811"
     CDS             506..>683
                     /locus_tag="ACG8MG_002811"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0376755.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSR"
BASE COUNT          245 a          102 c          120 g          216 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggt
//
LOCUS       DAZKNH010000248          640 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000248 DAZKNH010000000
VERSION     DAZKNH010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.258"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(240..606)
                     /gene="ssrA"
                     /locus_tag="ACG8MG_002812"
     tmRNA           complement(240..606)
                     /gene="ssrA"
                     /locus_tag="ACG8MG_002812"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          198 a          128 c          116 g          198 t
ORIGIN      
        1 gtttttcctt ccatcaacat aatgttcacc tcaaaaagag tatacgaact aatgttcttt
       61 ttgtaaagta ggaatatcat tgataatatg gatggaacat atatattaaa aagtgttctt
      121 tttgaatgct attttgaatg ccattttcat tcatttaacg aaaatctgcg aaaataaaaa
      181 atgctcaaac ccttataaaa taaggattta agcatctagc gaaatctgct gaaatctcac
      241 aatggagacg gcgggagtcg aacccgcgtc caaacacatc gccacttaaa aatctacgtt
      301 catagtcaac tcatttaagg attcacttta cagcttgccg agtgacaggc attctgtatc
      361 gctagtctga tgttctcttt ttcaacttac agacggaaag ttgaaacgta tcccactaaa
      421 gttaggaccc ttactcgagc acatgggcga tgccgagagg atctacgcta actgttttta
      481 ggcagctaaa gcgtaagagt tgttttcgtt tttagcagtt atatttaact gtaacgtttt
      541 tacgtagacg taacctacga aacgcaattc aagctcgact gtgcctgtcg aatccgtaac
      601 gtccccgtta gctaacaagg gttacgtgta tttttcatac
//
LOCUS       DAZKNH010000249          593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000249 DAZKNH010000000
VERSION     DAZKNH010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.259"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..580
                     /locus_tag="ACG8MG_002813"
     CDS             <1..580
                     /locus_tag="ACG8MG_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0376756.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          204 a          105 c          122 g          162 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cct
//
LOCUS       DAZKNH010000250          571 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000250 DAZKNH010000000
VERSION     DAZKNH010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.260"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..445)
                     /locus_tag="ACG8MG_002814"
     CDS             complement(146..445)
                     /locus_tag="ACG8MG_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0376757.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          186 a          107 c           68 g          210 t
ORIGIN      
        1 taattaaagg ataaaataca atataaagaa acatctgttt ctttatttag aataaaaaaa
       61 tacagactct gttcaaataa atgaacctag cctgcattat gagattgact atacttttta
      121 attcattgag atgaataact cgtttttata aatgcccaaa agtaatcggt gccgaagcca
      181 ttcctcgacc taattctttt tcaaattttc ctattgttga tatctccata taaaacggtg
      241 atactggttt agacaattct cccctgattg ctaacggacg taaactacgt tccaaattat
      301 ttatgacttt aggataatac tgaccttttt ctaagcctgt ctttgcgtct atcaaaagta
      361 tacgttcctt ttctttgatc tctggattta aaacgagatc atataatttt agtaccatgt
      421 tttctatttc ttcactttta gacatcacga tttcctccca aaaatatatt tcttcccact
      481 acattctata aacaaagtat acgctacatc tagatttttt taaatcatta ctggattgtt
      541 aaaacctttc aatacactgt tatggcaacg a
//
LOCUS       DAZKNH010000251          551 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000251 DAZKNH010000000
VERSION     DAZKNH010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.261"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..>551
                     /locus_tag="ACG8MG_002815"
     CDS             55..>551
                     /locus_tag="ACG8MG_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFQ0376758.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          202 a          102 c           90 g          157 t
ORIGIN      
        1 ccttctgtag aataaagtta acgacaacca attcacagaa aagaggactt ccctatgaat
       61 gattttacta cagaaattgt gcaaactcta gtcactaaag gcgatttaaa tgaattattc
      121 cgttcgcact tagaaaaagc gataaacaca ctcctacgga ctgaattaac ggctttttta
      181 gattacgaaa aatatgatcg cactggtttt aattcaggta attcgagaaa cggttcttac
      241 tttcgatcaa tcaaaaccga atatggtgaa ttaacattgg aaatacctag agatcgtaat
      301 ggtgagttta aacaacaaac tttaccagcc tacaaaagaa caaacgatac attggaaacc
      361 actattatcc atttattcga aaaaggtgtt acgatgtctg aaattgctga tttgatcgaa
      421 aaaatgtacg gtcatcacta tactccacaa accatgtcca acatgactaa agttctgact
      481 gaagaagtaa atgcctttaa atccagagcc ttaaatgata agtatgtcgc tatttttatg
      541 gacgctactt a
//
LOCUS       DAZKNH010000252          537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000252 DAZKNH010000000
VERSION     DAZKNH010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.262"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..365
                     /locus_tag="ACG8MG_002816"
     CDS             <1..365
                     /locus_tag="ACG8MG_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0376759.1"
                     /translation="VLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGD
                     AVDQEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELF
                     ISILKELEEHENWYAEVE"
BASE COUNT          211 a           78 c          117 g          131 t
ORIGIN      
        1 tcgtcttaca gacccaagta aaatcgatga gaaatgcgat gcaatatctt gagtatatgg
       61 acttttttgt gaaagaattt gatacaaatt tatatgttgc tggtgttatt cctttcatgt
      121 tggaaagcgg tgacgcagta gatcaggaaa tgtataaaca agcccaagag atttatgggg
      181 aacatttgat taaaaatgtt gtactcaaaa atgcgcgctt aaaacgatac gacggatcag
      241 gtattacgat ggagaagaca aagaaaggtc tcttaaaaca atgggatcgc aaaagccacg
      301 aattatttat cagtattttg aaagaattgg aagaacacga gaattggtat gcggaggtgg
      361 agtaattggc tgacatcaaa ggacttgata tttttgggaa caacaaagga ctcgccaaaa
      421 taaaaaaaga agcagaagca aagaaaaagc agcaagcaat tacaccatct gaaaataagc
      481 aattagaagt gcaccaagtt caagaaataa aaacttcctc caatgttgaa agacaga
//
LOCUS       DAZKNH010000253          517 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000253 DAZKNH010000000
VERSION     DAZKNH010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.264"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          175 a           66 c           70 g          206 t
ORIGIN      
        1 tagatataat acaaaaaggc aaagatattt tcaaaacatt ttttcttgat gtgatatcct
       61 tgtctttttt gtaatgtttt taacttattg cttttgcaaa atttatttac ctgttttctg
      121 gatcaacgat agtgtatatt atgctatcct tgtttggatt ttaggacgtt taaagaggca
      181 cttttttaca taacttaatc catagacctt ttaagcatgc tgtgagataa taataaagaa
      241 gagtttaaag cgcaccccaa accacttccc cataagtgta ttacgcttta aactctttta
      301 tatttgaatt cactaagaag cataccatat ttttgaaaaa aagtgagaaa aaaggcttat
      361 aattaggatt tagagtaatt aattagatgt ttattttata ggactgatat tatagattac
      421 aaatagtatc gtatttcgca atcttaaaat tatcttctta ctatgaataa gataataaaa
      481 tttgtatttg tcctttttag gtaacttatt ttaatct
//
LOCUS       DAZKNH010000254          514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000254 DAZKNH010000000
VERSION     DAZKNH010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.265"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..>514)
                     /locus_tag="ACG8MG_002817"
     CDS             complement(107..>514)
                     /locus_tag="ACG8MG_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFQ0376760.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          162 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcc
//
LOCUS       DAZKNH010000255          514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000255 DAZKNH010000000
VERSION     DAZKNH010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.266"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8MG_002818"
     CDS             complement(<1..132)
                     /locus_tag="ACG8MG_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0376761.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAZKNH010000256          506 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000256 DAZKNH010000000
VERSION     DAZKNH010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.267"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..>506
                     /locus_tag="ACG8MG_002819"
     CDS             83..>506
                     /locus_tag="ACG8MG_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0376762.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRS"
BASE COUNT          170 a           60 c           98 g          178 t
ORIGIN      
        1 atccttttgt ataataacaa ccaacctatt tacgagttgg ttgttaaagg ttattattta
       61 tttgtaaggg gggaagaaag gaatggatga aaaaacggaa ttaacagtca aatatcaaaa
      121 tgaattgaat ttagtaccgc tgaaaaattt caatgctaag cagatggatt tattttttgc
      181 attatgcgct cgtatgaaag atcgtggttt gaaaaaagtt agattttctt tcgaggaatt
      241 aaaagagtta agtgattata agatgactgc tacaaaagcc tttgtcggtg atttagaaaa
      301 gctctataaa gatatgctta atcttactta ccgtactgaa gatgaggatt ctattcgcta
      361 ctttgttttg tttactggtt ttgaaatcaa taaaacaaaa aagtttgttg aagtcagtgt
      421 taatccggac ctagaatata ttattaatgg tttaacagct gaatttagta gatttgaatt
      481 atctgctttc acgtcgttgc gttcta
//
LOCUS       DAZKNH010000257          468 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000257 DAZKNH010000000
VERSION     DAZKNH010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.268"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>468
                     /locus_tag="ACG8MG_002820"
     CDS             <1..>468
                     /locus_tag="ACG8MG_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFQ0376763.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAZKNH010000258          462 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000258 DAZKNH010000000
VERSION     DAZKNH010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.269"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACG8MG_002821"
     CDS             complement(<1..430)
                     /locus_tag="ACG8MG_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0376764.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAZKNH010000259          458 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000259 DAZKNH010000000
VERSION     DAZKNH010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.270"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>458
                     /locus_tag="ACG8MG_002822"
     CDS             <1..>458
                     /locus_tag="ACG8MG_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFQ0376765.1"
                     /translation="RPVSCGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQY
                     QRSIHDRPDNYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALD
                     IETALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          150 a           88 c           81 g          139 t
ORIGIN      
        1 aaggcctgtt tcatgcggta tgagaacact ttatcgttta ttttctaaag gaatatttga
       61 tattgacaca ctaccgatga aaggtaaaag aaaacccaat ggccatcagg aaaaacgggg
      121 aaaacaacag tatcagcgct caatccatga tcgacctgat aattatcctg atttcaattc
      181 tgagtttggt caccttgaag gtgatacgat cgttggcatt catcataaaa gtgccgtcat
      241 tactttagtt gaaagattat ctaaagtcat tatcacgatt aaacccaacg gccgtaaggc
      301 attagatatt gaaactgccc ttaatcaatg gttttctcgc ttccctaaaa acttctttaa
      361 atctattacg tttgactgtg gaaaagaatt ttctaactgg aaagccatta gtaaccaaca
      421 tgatattgat atatattttg cggaccctgg aacacctt
//
LOCUS       DAZKNH010000260          421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000260 DAZKNH010000000
VERSION     DAZKNH010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.271"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..355)
                     /locus_tag="ACG8MG_002823"
                     /pseudo
     CDS             complement(170..355)
                     /locus_tag="ACG8MG_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DAZKNH010000261          415 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000261 DAZKNH010000000
VERSION     DAZKNH010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.272"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..339)
                     /locus_tag="ACG8MG_002824"
     CDS             complement(<1..339)
                     /locus_tag="ACG8MG_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0376766.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAZKNH010000262          411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000262 DAZKNH010000000
VERSION     DAZKNH010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.273"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>411
                     /locus_tag="ACG8MG_002825"
     rRNA            <1..>411
                     /locus_tag="ACG8MG_002825"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DAZKNH010000263          400 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000263 DAZKNH010000000
VERSION     DAZKNH010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.274"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..390
                     /locus_tag="ACG8MG_002826"
     CDS             <1..390
                     /locus_tag="ACG8MG_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0376767.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          156 a           72 c           60 g          112 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt
//
LOCUS       DAZKNH010000264          373 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000264 DAZKNH010000000
VERSION     DAZKNH010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.275"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..343
                     /locus_tag="ACG8MG_002827"
     CDS             <1..343
                     /locus_tag="ACG8MG_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0376768.1"
                     /translation="DNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDL
                     IRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRA
                     SLYRKLSEVNN"
BASE COUNT          148 a           47 c           75 g          103 t
ORIGIN      
        1 cgataacata cgagataaaa aggcaagttt aaaatcacta aaagatacat ggcttgattt
       61 atcagaagat aatccataca gccaattctt aattactgta atggctggtg ttaaccaatt
      121 agagcgagat cttattcgga tgagacaacg tgaagggatt gaattggcta agaaagaagg
      181 aaagtttaaa ggtcgattaa agaagtatca taaaaatcac gcaggaatga attatgcggt
      241 aaagctatat aaagaaggaa atatgactgt aaatcaaatt tgtgaaatta ctaatgtatc
      301 tagggcttca ttatacagga aattatcaga agtgaataat tagccattct gtattccgct
      361 aatgggcaat att
//
LOCUS       DAZKNH010000265          368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000265 DAZKNH010000000
VERSION     DAZKNH010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.276"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..318)
                     /locus_tag="ACG8MG_002828"
                     /pseudo
     CDS             complement(82..318)
                     /locus_tag="ACG8MG_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          120 a           56 c           65 g          127 t
ORIGIN      
        1 aaaaagatgt tggtgaagtt caccaacatc aaaaagtata gagccgatag aaggactgac
       61 cttctgtctc agattttttt gttatttcac ttgaatcaat tgattgatca aaaaaatcct
      121 gattttcatg ttctcaaatg atttaaatcc ataagataat cgtttcagtg tcttgatatg
      181 gggaatcttg gcctctattt tgccattaga atacgggtaa atcatagcat tgtaaatacc
      241 ttcttcatac gttaaaaggt tttgaagttt cgtgcgaagt tgtgcatcaa gtgtttcagg
      301 aagatttttt aataacgccg tttgtataat taagcgagac aaataaaaaa gccatttatg
      361 ttacactc
//
LOCUS       DAZKNH010000266          368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000266 DAZKNH010000000
VERSION     DAZKNH010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.277"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..272)
                     /locus_tag="ACG8MG_002829"
     CDS             complement(<1..272)
                     /locus_tag="ACG8MG_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376769.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          106 a           69 c           52 g          141 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcataa
//
LOCUS       DAZKNH010000267          362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000267 DAZKNH010000000
VERSION     DAZKNH010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.278"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>362)
                     /locus_tag="ACG8MG_002830"
     rRNA            complement(<1..>362)
                     /locus_tag="ACG8MG_002830"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DAZKNH010000268          341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000268 DAZKNH010000000
VERSION     DAZKNH010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.280"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>341
                     /locus_tag="ACG8MG_002831"
     CDS             <1..>341
                     /locus_tag="ACG8MG_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0376770.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DAZKNH010000269          339 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000269 DAZKNH010000000
VERSION     DAZKNH010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.281"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..222)
                     /locus_tag="ACG8MG_002832"
     CDS             complement(25..222)
                     /locus_tag="ACG8MG_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0376771.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
BASE COUNT          111 a           71 c           34 g          123 t
ORIGIN      
        1 atttgcttag catttttttt cattttagca cttccctgga attgctccac ctaaaaaacc
       61 acctatagac attcctgcaa tacaagtagt tgccttggcc caatcgaccg tacatttatt
      121 tttagtgcaa tacactccat ttccataata ttttccacta tgagtggtac ccccatagat
      181 aagttgtgtc tctttaatag acaaaatttt taaatgtttc ataatctaat cacccctaaa
      241 ttaatgtttt tagactatta ttctaacatt cattaaattt caaaaaacaa ttccttcttg
      301 aaacgtagca aaataattcc tgaataagaa tttaatcac
//
LOCUS       DAZKNH010000270          335 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000270 DAZKNH010000000
VERSION     DAZKNH010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.282"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>335
                     /locus_tag="ACG8MG_002833"
     CDS             <1..>335
                     /locus_tag="ACG8MG_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFQ0376772.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGER"
BASE COUNT          125 a           53 c           77 g           80 t
ORIGIN      
        1 ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga
       61 acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat
      121 caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc
      181 ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc
      241 ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga
      301 tccggaaagc ggctactacg tcaaaggcga acgag
//
LOCUS       DAZKNH010000271          323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000271 DAZKNH010000000
VERSION     DAZKNH010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.284"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8MG_002834"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8MG_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFQ0376773.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DAZKNH010000272          321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000272 DAZKNH010000000
VERSION     DAZKNH010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.285"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>321
                     /locus_tag="ACG8MG_002835"
     CDS             <1..>321
                     /locus_tag="ACG8MG_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFQ0376774.1"
                     /translation="TQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTK
                     TEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFG
                     FSVST"
BASE COUNT          107 a           69 c           63 g           82 t
ORIGIN      
        1 acgcaagcag cctatgcttt cttaaaacga ctccataaac agtttggtga gccgaaagca
       61 attgtgaccg ataaagcacc ttctcttggc tccgccttta gaaagttaca gagtgtgggt
      121 ttatatacta agacagagca ccgaactgtg aagtatctta acaatttaat agaacaagac
      181 catcgaccta ttaaacgacg aaataaattt tatcaaagtc tccgtacagc ctcttccacg
      241 attaagggca tggagaccat tcgaggaata tataaaaaga accgaagaaa tggaacgctc
      301 ttcggctttt cggtgtctac t
//
LOCUS       DAZKNH010000273          321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000273 DAZKNH010000000
VERSION     DAZKNH010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.286"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACG8MG_002836"
     CDS             complement(<1..>321)
                     /locus_tag="ACG8MG_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0376775.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAZKNH010000274          315 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000274 DAZKNH010000000
VERSION     DAZKNH010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.288"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>315
                     /locus_tag="ACG8MG_002837"
     CDS             <1..>315
                     /locus_tag="ACG8MG_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376776.1"
                     /translation="HWNAIEGKPTTVKGDKGDPGQAATITIGTVSSGSTASVTNVGTS
                     SAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDKTKLDGIAAGAQKNPGNAT
                     TTT"
BASE COUNT          109 a           62 c           72 g           72 t
ORIGIN      
        1 tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag gcgatcctgg
       61 ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt ctgttacgaa
      121 tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag ataaaggtga
      181 tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg ggttgatgtc
      241 gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa atccaggtaa
      301 tgctacaact acgac
//
LOCUS       DAZKNH010000275          309 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000275 DAZKNH010000000
VERSION     DAZKNH010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.290"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..309
                     /locus_tag="ACG8MG_002838"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACG8MG_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAZKNH010000276          292 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000276 DAZKNH010000000
VERSION     DAZKNH010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.293"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           95 a           56 c           34 g          107 t
ORIGIN      
        1 tagaagcatg taattttttc tcagaaacaa accactcatt ggaaaagaca tgaatcaaat
       61 catcacttac ttgtgtaaaa tagcgacgtt taatgacaaa gatatcttca ttgctatcta
      121 gtgtaccttc atttctttgt gcttaaattc aaagataatt ttctcaataa agatttttga
      181 ttccagtaag attcctttac tccttttaca acatagcgtt gtcctttgtt ttgaaacatc
      241 ttcttcccct ctttgccatt aaaaatagcc caattctcta taaagaagat ta
//
LOCUS       DAZKNH010000277          291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000277 DAZKNH010000000
VERSION     DAZKNH010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.294"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..186
                     /locus_tag="ACG8MG_002839"
     CDS             <1..186
                     /locus_tag="ACG8MG_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0376777.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
BASE COUNT           97 a           52 c           59 g           83 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat g
//
LOCUS       DAZKNH010000278          281 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000278 DAZKNH010000000
VERSION     DAZKNH010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.295"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..>281
                     /locus_tag="ACG8MG_002840"
     CDS             28..>281
                     /locus_tag="ACG8MG_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0376778.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
BASE COUNT           84 a           53 c           56 g           88 t
ORIGIN      
        1 tgaaaacgaa tgcaaggagt gatagctatg atgtctaaaa acaatacgaa tggtagaaat
       61 caatttgcga tgcttacgat tgatgatcta gttcctcaag atcatctcgt tcgaaagatt
      121 gatgccgcgc ttgattttga atttatttat ccaatcgtcg aagccactta ctccgattta
      181 ggtcgcccaa gtatcgatcc agtgattctg atcaaattgg tgtttattca gtatttattc
      241 ggtatccgtt ccatgcgaca aaccattaag gaagtcgata c
//
LOCUS       DAZKNH010000279          264 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000279 DAZKNH010000000
VERSION     DAZKNH010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.296"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(3..>264)
                     /locus_tag="ACG8MG_002841"
     CDS             complement(3..>264)
                     /locus_tag="ACG8MG_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0376779.1"
                     /translation="PSNRFVKDFYKVWDSEVFFLAIEDSLLINLYYSNKNYFKIPATK
                     TRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT           88 a           51 c           38 g           87 t
ORIGIN      
        1 ctttaatctt tatacctttc tggatcaacg aaagtatact tcaaatagtc ataacgccga
       61 tgatcgctcc gtgcgtctgg cacatcagtc acgatatcaa acaaaaaata cacatctttc
      121 ttcattctgg tttttgttgc cggaatttta aagtagttct tattagagta atagagattg
      181 attaataagc tatcttcgat tgctaaaaag aaaacttctg aatcccacac cttataaaaa
      241 tctttgacaa atctattcga agga
//
LOCUS       DAZKNH010000280          241 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000280 DAZKNH010000000
VERSION     DAZKNH010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.297"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..220)
                     /locus_tag="ACG8MG_002842"
     CDS             complement(<1..220)
                     /locus_tag="ACG8MG_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0376780.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFP"
BASE COUNT           63 a           45 c           46 g           87 t
ORIGIN      
        1 caggaaaaat gatttcggtt ctcccacaac gtcgcttatt ttctttgtat tgttcaaagt
       61 attcaagtgc tgttccacca catttgagaa agttatagac attgtaaatt gtttggcgtc
      121 cacgtttaag ctgcttcgca acgatagcaa ccggagtttc ttgatgaaaa tatgcttcta
      181 tcattacaag ctcgtttggt gtaagatggg tataagtcat ttatgttcac tctccttgta
      241 t
//
LOCUS       DAZKNH010000281          233 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000281 DAZKNH010000000
VERSION     DAZKNH010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.298"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           79 a           35 c           45 g           74 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta cactcttagt
      181 aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac ttt
//
LOCUS       DAZKNH010000282          232 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000282 DAZKNH010000000
VERSION     DAZKNH010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.denovo.300"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>232)
                     /locus_tag="ACG8MG_002843"
     CDS             complement(<1..>232)
                     /locus_tag="ACG8MG_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0376781.1"
                     /translation="LLNDVSNYKTILRINKQRFKCKHCGKTFLAEDSVSDRRCSIARR
                     VKQAILELLSEPLSMSLIARMKHISPTTVIRIL"
BASE COUNT           70 a           42 c           53 g           67 t
ORIGIN      
        1 aagtattcga atgacagtgg tcggagaaat gtgcttcata cgtgcgatca aagacataga
       61 aagaggttcg cttaatagct caagaatggc ttgtttgact ctgcgagcaa tggagcagcg
      121 acgatcacta actgaatctt ctgctaaaaa agtctttcca cagtgtttac atttgaagcg
      181 ctgtttgtta atccgcagaa tggtcttgta gttactgaca tcattcaata ag
//
LOCUS       DAZKNH010000283         5967 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.guided.118, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000283 DAZKNH010000000
VERSION     DAZKNH010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.guided.118"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            213..1109
                     /locus_tag="ACG8MG_002844"
     CDS             213..1109
                     /locus_tag="ACG8MG_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0376782.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1259..2200)
                     /locus_tag="ACG8MG_002845"
     CDS             complement(1259..2200)
                     /locus_tag="ACG8MG_002845"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0376783.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2326..3447
                     /locus_tag="ACG8MG_002846"
     CDS             2326..3447
                     /locus_tag="ACG8MG_002846"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0376784.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3705..5207
                     /gene="eat(A)"
                     /locus_tag="ACG8MG_002847"
     CDS             3705..5207
                     /gene="eat(A)"
                     /locus_tag="ACG8MG_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0376785.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5274..5801
                     /locus_tag="ACG8MG_002848"
     CDS             5274..5801
                     /locus_tag="ACG8MG_002848"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0376786.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1967 a         1043 c         1236 g         1721 t
ORIGIN      
        1 atatttaaca atgactaatg ataacaaatt ttcagacaat tacaactatt cagaaaactt
       61 ttaggcgaat tttaatagaa gaatgattta tttcatcata ttttcataca aatgttaaat
      121 tttcatacaa atataggaaa cgttttactg gctttttact atttaaacag gctatcatag
      181 gaaaaagtga cagagaaagt gaggcggaaa agatgaacaa aaatgacgta ctgctagttg
      241 caaaaaataa atcactttat tttttgcctt ccatccgctc tttagatgtt tggtccggaa
      301 aagatttact acagtcggaa ttcattaaat cagctagaca acagttggca aattatcagc
      361 tgatcgtttt tttagattat ggatttgata tggagatggc tcgacgcgtg cgcccattta
      421 ccaatgcaaa aattgtgtta tttttttgga atcacttcaa agcagaacac gaggcgatgc
      481 tgaagcaggc acaagatgaa ccggcaatcg atgagattta tcattttgat tttttggaag
      541 caaaagaact tgggctgaaa cataatagtt cattttattc gaaacatatg aatttttctt
      601 ctagcgaacc aaagacggat ctattttttg gtgcaacgaa taatgggaga aaagaacgag
      661 cagaaagcta taagcaggaa tttataaaaa gagatgttac gaccaactat ttcattcttc
      721 cacatcgagg gaatgaacaa cctggatatc ttacttatga agagtacctg aaaaaaacag
      781 gggaatcgcg cgggattctg gaactgttgc gggagggtca gcaaggagtg actttgcgga
      841 catttgaatc catgtatttt caaaagaaac tagtgacgga taatcaagcg attgctcatt
      901 atcattttta tcgtcctgaa aatatttttt tgcttcgaga acgtgattta gatgaattac
      961 cggaatttct gcatacacca tttaagccaa tcctatcaga gctattggaa ttttatgatg
     1021 cagaaaattg ggcaaaacga tttatcacta acgattccac tatttatgag caatacgaat
     1081 atgacctctc ctctgttgaa aacaaataaa aaacctacat tttgaagaga ttatgtactg
     1141 aaacaaacaa gcacaattct tatggcatca tgagagttgt gttttctttt tgatctttta
     1201 ttgggcttaa aaaaagagaa ccagctgctg attcagctga ttctcatcag agagcctttt
     1261 attctgcgcc aaggattcct tcttttgcat ggactaattg ggtcaaaaag gcacaatatg
     1321 gtgtagcaat atcatatttt tgtccttttc gccaaatggc accattgatg tagtcgattt
     1381 cagtcaaacg atggtttttg atcaggtctt ggtacataga tgggtaatgc aacccgatgt
     1441 tgtcaggatc ataagtagaa gcaatatgtt ggtacacttc ttcttgatct agtatgattc
     1501 cttcttttgc tgcgatagaa gcaaattcag atacgattgt tcgaaccatg ctctctgccg
     1561 ctttttgcgc gcctagttca gcaatattac aatccaaaat cgtgcataaa ccattcaatg
     1621 ttccatttac acaagctttt cgccaaatgg aataacgaac gttatggcta tagatcggat
     1681 ttaaatttgc ttcttgaaag acttctacaa ctttttttgt aaatgcttcg ccttctggtt
     1741 ccaagttctc tagttcgatt tctccatcac ctaacaatgt gacttttccg ggaccagcta
     1801 gaccagctgt ccacatcgtg atcccaaata agatattttt ctttggcaca tatttttcca
     1861 gtacatcttc atgacctaat ccatttagta aacacaaaac ataagtattg tcagtaataa
     1921 tggattggat cgaacaaaac atatcatcta attgattggc ttttgttaat gctacgatca
     1981 agtccacgtg ttcgttgctt tcgatgattt cttctggtga atagattggc agttttgcga
     2041 ccacttcttt accgttaaag tcagcaatca acccatttgt acgaatggct tcaatgtgag
     2101 caggccaacg atcgatcaag aggacctcat ttccgctttg atgtagcatc aatccgattc
     2161 ggctgcccat tgctccggcc ccagctattg cgattttcat gttttttctc tcctttattc
     2221 aatgagttcc cttttagtat atagaaaaat ggaagaaaat agcattgttt tgtttgaaaa
     2281 aaatgaaaaa aagtgtctta cttaagaaga aagtgaggga tacagatgga tattttggcg
     2341 gcaattgatt catttaaagg aagtgcttct tcatttgaac taaatcaggc agttctaaat
     2401 ggctgggaag atcgtaaagg aagaaaaatc aatgtgccga ttgcagatgg cggagaaaat
     2461 actgcagaag caatctatta cgcgctcagg ggaacatggc gtaccattga tgggatggat
     2521 ctcttattcc gtcagcgaaa aatcagctat ttattgacaa cttggcaagg aaaaaacacc
     2581 gcagtcatcg aatctgcaca agcattgggg ttagatctac ttgataaacc aacggatcaa
     2641 actatccgac ttgcttccag ttatgcctta ggagaactta ttaaagatgc actgcttcaa
     2701 gaagtagaac aaattattgt cactttagga ggaagcgggt gtagcgatgg tggtctagga
     2761 ttactccaat ccttgggtgc ttctttagaa gggtacacag aaggaaatcc tttattatct
     2821 actaaaaaac ttcattttga tcgaagaaac gttcttgttt ctaaagtaaa attgattatc
     2881 gctgcagatg ttaccaatcc atatacaggt caacaaggag ctgcttggca atttggaaga
     2941 caaaaaggag gaatagatac gacgctagct ttcttagata aacaagcagg aaagattgct
     3001 cagcaaataa aagatttctg ggaggtggat ttagatgata ttccaggaag tggagcagca
     3061 ggcggtattg gtggagccct ttttttgttg ggagcgaaga tggtttctgg tttcgattta
     3121 gtcagccaaa tcactggatt ggaaaaaaag atcgaacaag cagatgtcat cattacagga
     3181 gaagggcgga tcgatcagca aacgatccac ggaaaagttc catacggtgt agcagtgctt
     3241 gcaaaaaaat atcgaaaacc aataatcgct atttgcggaa gtagagacca agagctagaa
     3301 ggactttctg attttctccc aattgtctta agtattcatt tgggtccaat aggtttagaa
     3361 gaagcaatgg aacacaggca aacattagaa aaaacaaaaa tcttaggaca gacacttagc
     3421 agatttttca ccatcctccc gaagtagttg acaaaatcat tttctttatg ctatcgtaat
     3481 ttcaaatata ggacaaagtg agggatccgt gcattgaaaa attaagtcga ttgatgaaag
     3541 ctgcattcag actgtacctt gtggagacaa ggaagcagtg tgcagatttt cgggattggc
     3601 ttggtttttt cgatgtatgg gtctttttgt gtgtatccaa cccttgcatc ctctttagcc
     3661 agttctgaaa ataggactgg tcttttttta cgaggtgatg gaaaatgtct aaaatcgaaa
     3721 taaaaaatct gacattcggc tacgacagcc aaggcacatt attatttgaa caagcaaatc
     3781 taaattttga cacacaatgg aaactaggac ttatcggacg aaacggtcga ggaaagacaa
     3841 ctttactgaa tattctacaa aacaaactac cttatcaagg gcaagtaatc catcagcaag
     3901 aatttgccta tttcccgcaa cagacaaaag ataaagaacg tttaacctat tacgtgttaa
     3961 atgatattac ggattttgag atatgggaaa tcgaaagaga gctccaattg atgcaaacag
     4021 atcctgaaat cttatggaga gaattcagca cactatcggg gggagagaag acaaaagtcc
     4081 tactggcact tttatttgtg gatgacactc atttcccgtt aatcgatgaa ccaacgaatc
     4141 atttggatat ctctggtaga aaacaagtag cggcttattt gaaaaagaaa aaacaaggct
     4201 tcatcgtggt cagccatgac cggggattta tcgatgaagt agtggaccat gttttagcaa
     4261 tcgaaaaaag tcaactggaa ctttatcaag ggaatttctc tatctatgaa gaacagaaaa
     4321 aacttcgtga tgaatttgaa atggctcaaa atgaaaaatt gaaaaaagaa gtcagtaggc
     4381 taaagaaaac agcagctgaa aaagccgaat ggtctcgttc ccgagaagga gataaaacaa
     4441 agaaacaagt cggattcatc gatactgaat ctagacgagt gaataaagga gcagtgggtg
     4501 ctgatgctgc acggacgatg aaacgatcca aagcaatcgt gaatcggatg gagacccaga
     4561 tcagcgagaa agaaaaacta ttaaaagata tcgaatatat cgattctttg acgatgaata
     4621 gccaagcgtc tcaccataag cgacttttaa gcgtagaaga tcttcaatta gggtatgaaa
     4681 atctgttatt cgagccaatt cattttacaa tcgagcctca tcagcgggtg gcgatttcag
     4741 gtcctaacgg tgcaggaaag tcatccatta tccattatct tctgggggca ttcaacggca
     4801 aggttatagg agaaaaatac cagccaaaac atctgagcat tagttatgca agccaaaatt
     4861 atgaagacaa tcgaggaacg ttggcggaat ttgcagagaa aaaccaagta gactaccaag
     4921 catttttgaa caacctccga aagcttggga tggaaagaga tgtttttcat aacaagatcg
     4981 agcagatgag tatgggccaa cggaaaaaag tggaattggc taaatcttta tcacagccag
     5041 ctgaactata tacatgggat gaaccattga attatttgga tgtcttcaat caagaacaat
     5101 tagaacaact gatcttgaac gtgaaacctg ccatgttact agtggaacat gatcaaacct
     5161 tcctggataa agtatctact gagattattt ctcttgagag aatctaaccc ttgtaatctc
     5221 aagaacaaca aggtatggtt aaggggaact ttgccaacga aggagagaat cacatgcttt
     5281 atgtaacgag acacggagaa acaacttgga acgcacaagg attagtttgc ggacgggcag
     5341 atgtgccgct gactgaaaaa ggacagatgc aggcacagaa actagcagaa aaggtagtag
     5401 atttgcctgt tccaatcacg aaaatcatcc attcccctct tcaacgagca agagatacag
     5461 cacaagcagt tgctgatcgt ctcagcttgc cgctgaccgt tgatgaacga ttggtagaaa
     5521 tggattttgg ggattatgat ggactgccaa gcaaagatga gaactttcaa aaagcacgtt
     5581 tagcgttcgc tgtacgattc ccaaatgggg agtcagtctt agatgtgtat gcacggatcg
     5641 ttcctttatt gaaagaatgc atcgaagacg aagaaaatgt ctatctgtta gtttgccata
     5701 atgccttgat aagagtgatc aatgcttatt ttcatccgat gcctaatgag gggttcttta
     5761 cattcatggt agataataca gaattggttt cttatgagta attatgtttt tgtgttattt
     5821 aaataaaata tttcaaggca ctgcttttgc ttagcagtgc cttttttagg ttatgacaat
     5881 tcttttgata atataaactc aacctttaga aatgaaattt aattatttta ataaaagtat
     5941 tgaaattttg tttctttaga tatatac
//
LOCUS       DAZKNH010000284         5879 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.guided.122, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000284 DAZKNH010000000
VERSION     DAZKNH010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.guided.122"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            398..862
                     /locus_tag="ACG8MG_002849"
     CDS             398..862
                     /locus_tag="ACG8MG_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0376787.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            846..1475
                     /locus_tag="ACG8MG_002850"
     CDS             846..1475
                     /locus_tag="ACG8MG_002850"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0376788.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1829..3307
                     /gene="msr(C)"
                     /locus_tag="ACG8MG_002851"
     CDS             1829..3307
                     /gene="msr(C)"
                     /locus_tag="ACG8MG_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0376789.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3354..3800
                     /locus_tag="ACG8MG_002852"
     CDS             3354..3800
                     /locus_tag="ACG8MG_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0376790.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3999..4514)
                     /locus_tag="ACG8MG_002853"
     CDS             complement(3999..4514)
                     /locus_tag="ACG8MG_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0376791.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4631..5833
                     /locus_tag="ACG8MG_002854"
     CDS             4631..5833
                     /locus_tag="ACG8MG_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0376792.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1817 a          980 c         1240 g         1842 t
ORIGIN      
        1 ctatttttct tgcacctttt acttgactaa tcgtaggaaa aaggttgtga caacatcttg
       61 tcataacttt tttattactg tttaagcata ctgatccaat ttctaatatt cgacgggatt
      121 cttgcaaaag gcgatttcgg aaatttttgg cggtgtaaaa ttatctactg cagaagagct
      181 atcttctata gtttgataag aatcaataat cttggtttta gcgttgtgct ttctttatca
      241 ttttttccgt tcattctaca aaaaatttct tttctctata cttttctatt gagagcgttt
      301 tttttattac acagctgatt ctttgacttt tttctttttt tattctatta ttgacaagtc
      361 aatcgttgac gagtcaatta ttagaaagga ggaaattatg tctttagaag agattagtca
      421 ccttttatat caaatcaaat tagccgatca aaaaatcgtc cagatctttg aagaaaatat
      481 cggtataagt cttactagat acgagatcat gatgagactg aaagaagaaa atccattgct
      541 acaaagcagt ttgcaagaaa agttgcagat tgatcaagga gcagttactc gtcatctgaa
      601 ggtattggaa gcaaatggct atgtttcgcg tcagagaaat cctcagaaca accgggaagt
      661 gtatgtagcg ctaacagaaa aagcccagaa gaaaatcaag cattgtgaaa tggaccataa
      721 agatacaggt tccgtgctat ccccggcttt cagcgatgta gaaatgaaac aatttctaac
      781 acttttagat aaattgaata cccaactttg caatataaca acagaaacga aagaggttaa
      841 taataatgga acaaaaaaat gatttttcaa caattatgac tggtagaaga tccgttcgcg
      901 tatatgatga aacatacaaa atcccacacg aagaaatgct ggaaatgatc aaagaagcga
      961 cgactgcacc ttcttctgtc aatatgaaac catggcgctt tgtggtcgct gaaagcgagg
     1021 aagcaaaaga aatcttgcgt cctttgatcc gtttcaatac gagacaaaat gatacttctt
     1081 ctgcaatggt catgattttt ggcgatatgc aatgttacga atatggcgaa gaaatctaca
     1141 accaagctta cgaatcaggg aagatgccaa aagaagtcag agatcaacaa ttagctgcga
     1201 tcattcctta ttacaagagt ttttcaagag aagaaatgaa cgatgtagtc aaagtcgatt
     1261 ctagcttagc ggctatgcaa ttcatgttag ttgcgcgtga tcatggctat gaaacaaatc
     1321 caattggtgg attcgaagca gatcagttgg cagaggcatt tggtttggat aaagaccgtt
     1381 atgttccagt tattatttta tcaataggaa aagcggtaga agaaggttac gagtctgttc
     1441 gtttagcacc agaaaagatc actatgttcg aataaaaggt tttcagttac tattcagtag
     1501 ctattgcttt ttttgaatcg tgtgtggtaa cctaataaaa aaggaggtcg gaaaaatgac
     1561 tgcatcgatg aaattacgtt tcgaactttt gaataacaac taaataaatt caaaagacat
     1621 gtataaacaa caatttcatg gatggagtat gtccattttt tcgaacgaca gtagaatagg
     1681 tgaaagctat gacagatttg gtgatttagt catagttatt tcgttcagag taggaaaaga
     1741 tgtgatgcag tttttttgca aacagatttt tctgctcttt tttagtatcg ataattgttg
     1801 ttttacatga aataggagtg taaaacatat ggaaaattta gcagtaaata taacaaatct
     1861 acaagtcagc tttggcaacc agctagaact atctattgat tctcttcgtg tctatcagca
     1921 agatcgtata gggatcatag gagaaaatgg tgtcggtaaa tcgactttgc tcaaactaat
     1981 agccggtgaa ctttttcccg atcatgggaa aatccaaaca gagatcacct tcaactacct
     2041 gcctcaatta acctatctcg ctgaagcaaa ggacctaaat ttggaattag ccagtcattt
     2101 ccagttaaga ctggaagaaa cttcagagcg gaaatggagc ggaggggaag aacgaaagat
     2161 cgagttgata cgtcttcttt cttcttatga acaagggatg cttctagacg agccaacaac
     2221 ccatctagat agaaaaagta ttgatcgact gattgaagag cttcgttatt attatggtac
     2281 gctggttttt gttagtcatg atcgctattt tctagatgag ctagcatcga aaatctggga
     2341 agtaaaagac ggagaaatcc gagagttttc ggggaattat agtgcctatc tcactcaaaa
     2401 ggaattggag aaaaagactc agctacgaga agcagagtcg atcatgaaag agaaaaaacg
     2461 attggaaaaa tcgatccaag aaaagaaaaa acaagcggaa aagttggaaa aagtgtccag
     2521 taaaaagaaa aagcaacaaa tcagactgga tcggttgtct tcctctaaac aaaaagacag
     2581 tgtacaaaaa gccatccaaa agaatgcgaa aacattagag agaagactcc aaaaaatagg
     2641 agaaacaacc aagccgcagc agatgaaaca aatccgtttt ccagtaccaa aatctcttga
     2701 actccacagc cgctatccca tcatgggaca aaatgtccaa ttggaaagaa gtgggagaac
     2761 attactggta aatggtgatt ttcagttttc tttaggtaaa aaaatcgcga ttgtcggtga
     2821 aaatggttca ggtaagacaa ccttattaga acatatccgc aaacaaggag aaggaatcct
     2881 tctctctccg aaagtaagct ttcaagtata tcagcaaaag ggttatcaaa tgacatctga
     2941 agaatccatc attcgttttg tcatgagaca aacagagttt tcagaatcgc ttgtccgtag
     3001 tttgctgaat cacttagggt ttgctcagga aactttgacg aaaccgttat gtacattaag
     3061 tgggggagaa gcgacccgtc tgacgattgc tttgcttttt actaagccaa gtaatgtgtt
     3121 gctgttagat gaaccgacta attttattga tatggcaacg atcgaagctt tagagaagct
     3181 aatgcaaata tatccgggaa cgattttgtt tacttctcat gattcttact ttgtcgagcg
     3241 tacggctgat gaagtttatg aaataaaagg gcagaaaata aaaaaagtac ttacgagaaa
     3301 tttttaaatg tgctacgatg attttcgtga caaatttaaa ggagtggtac agattgccta
     3361 caaataaaaa agaaggaata atttttacaa caatgatgtg ttttttgatg gtcttgggga
     3421 tgagcgcgta taatttatgg ctgcatcaag atcttcattt atcaagtttg ttgattggtc
     3481 tagtgcctgg attcgtggtg gctttcattt tggatgtctt catcatcggt gtgctggcga
     3541 aaaaaattgc ctttaaacta ccagtaaatc cagaaagcaa ggtccaaatg atcttaacta
     3601 tttcatgctt gatggtttta ggaatggtga catgtatgtc tttattcggt gtgatcatgg
     3661 aacaaggaat acctgatcaa ttattctctg cttatatgaa tgcttggaaa atgaatgtga
     3721 ttgccgcttt accgttgcag ttgatcatcg ttggcccgct ttcaagaagt gtgctaaaag
     3781 caattcaaag taatgaatga tggaacactg ttatttgaaa aaaggccttg gcatgctttt
     3841 tgtcatagtc tcttgaatgc agttctagaa aaatagtgat ccgaacgata ggttttggcc
     3901 tgtcgttcgg atcgcttttt cttttccaac tgtattgctc ttttattgaa gataatttgc
     3961 tcttcaatag atatttctag aaaggttctg tttatccctt actcaaaaaa ctgctgcatt
     4021 tgttctctta cttgctgaca ccattggagt tcctgctgac aaagcataat tctgcgctgg
     4081 atcacaacat aacgtccaaa ttcttgtttc ttgtctagcg agaggtcatt caattttttt
     4141 tgattttttt gcatgatttt gttcagctgt tgttctctag tgaatagcag tccagtagct
     4201 gttgttttat cgagcgtcga aaaagcatag atttttgcta gaaattcatc tttttgcgta
     4261 ggagcgggag tttcctcttt gatccattcc tctacaagag cacgtccttc atcagtcaag
     4321 tggtagatct ttttttgaga gtgctcgtct ttgtccaaaa cggtcacata accctgctgg
     4381 tctaacttgg caagcgttgt atagatctga ctgtgatggg cttcccaaaa aattgtgatc
     4441 agctgcttca attcataccc agaacgtggt ttggataaaa gcacgcataa taatatatag
     4501 gctaaagtat tcataaaaat cctacctctc gtcttatttt ctttattata tcacagtaaa
     4561 gtttccacgt tttacatgtg catattgaca tgtaaaataa atcatgtatg atgctaatag
     4621 agagaaaggg atggaaatat tggagaatca aatagatata caagaacaaa caaatgaaga
     4681 aacaaagggg aaaagttcag cgctagttgt agcagctacc tgtatgatcg cttttatggg
     4741 agtgggtcta gtagacccaa tcttaaaaac gattgctgca cagttaaacg ctacgccagc
     4801 tgaaacgacc ctgttattta ctagttacat gttagtcact ggtgtggtga tgctatttac
     4861 aggtttctta tctagtcgga tcggtttgaa aaaaacattg atgacaggat tgtttatcat
     4921 cgttgtattc gctggtcttg gcggattatc tgggaatatc ggaatgctga ttgggttacg
     4981 tgccggctgg ggtgtcggga atgctttatt tgtctctact gctttagcaa cgatcgtcag
     5041 tattttagtt ggtaacacag aaaaagcaat catgatgtat gaagcagcac ttggctgcgg
     5101 aatggctgtt ggccctctag tcggtggggt cttgggcagt ggttcatggc gttatccatt
     5161 ttttggggta gcatttttga tgttttgcgg ttttatagcc ctagcggtac ttttacctga
     5221 aactgagaaa ccaaagagaa aagtaggcct ttgggaaggt gtcaaagcgt taagcaatca
     5281 taaacttcgt tcagttggac taatcgcatt gttatataat ttcggattct ttactttact
     5341 tgcgtatgcg ccatttttat tgattggcta ttctgaatta gaagtcggtt ttgtattttt
     5401 tggctggggt gtgcttctgg ctattgcttc gattttcgtt gcaccggctc ttgagcggaa
     5461 gtttacgact tatcggacaa ttttagcagc atttgtctta ttcattattt gtttgctgtt
     5521 attaggagct ggaacttcta tgccagtcct tgtaccgatc agcattgtgt tagcaggatt
     5581 tttccaagga attatcaata cactgttgac gacgattgct atggaaatac caggtctgca
     5641 acgaaacgtt gcttcgtcca gctatagttt tgtccgcttt ttcggtggag ctttagcacc
     5701 atttattgca ggtgtgattg gtgaacatat tggtgaaagc tatgcgtttt attttgcagg
     5761 tgccattgtt cttatcagtg tatgttgtat catttatcac cgtaattatt ttgctacaaa
     5821 tgaagaagaa taagaaatca gaggttgtga aaaagctgtc ctgcatcaag tcataagaa
//
LOCUS       DAZKNH010000285         4802 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.guided.135, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000285 DAZKNH010000000
VERSION     DAZKNH010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.guided.135"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8MG_002855"
     CDS             90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8MG_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0376793.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            763..1917
                     /locus_tag="ACG8MG_002856"
     CDS             763..1917
                     /locus_tag="ACG8MG_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0376794.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2132..3100
                     /locus_tag="ACG8MG_002857"
     CDS             2132..3100
                     /locus_tag="ACG8MG_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0376795.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3093..4124
                     /gene="vanA"
                     /locus_tag="ACG8MG_002858"
     CDS             3093..4124
                     /gene="vanA"
                     /locus_tag="ACG8MG_002858"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0376796.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4130..4738
                     /locus_tag="ACG8MG_002859"
     CDS             4130..4738
                     /locus_tag="ACG8MG_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0376797.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1481 a          921 c         1119 g         1281 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcct ataatctgat aaattattaa
       61 gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt gtggatgatg
      121 aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat acggttttca
      181 aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt gaccttgcca
      241 tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa ataagggaca
      301 agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat aaaattacag
      361 ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg gagttaattg
      421 ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag gagcagaacg
      481 aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag tgttatctga
      541 acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc ctctgtgaaa
      601 acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc gacgaatatt
      661 tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg
      721 acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa
      781 aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt
      841 gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg gaaacttggg
      901 gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa
      961 ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt
     1021 agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata
     1081 aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa
     1141 atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag
     1201 gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt
     1261 aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg
     1321 ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa
     1381 cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca
     1441 aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag
     1501 ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc
     1561 gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt
     1621 gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa
     1681 ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat
     1741 aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca
     1801 aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg
     1861 acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag
     1921 atgtatataa ttttttagga aaatctcaag gttatcttta ctttttctta ggaaattaac
     1981 aatttaatat taagaaacgg ctcgttctta cacggtagac ttaataccgt aagaacgagc
     2041 cgttttcgtt cttcagagaa agatttgaca agattaccat tggcatcccc gttttatttg
     2101 gtgcctttca cagaaagggt tggtcttaat tatgaataac atcggcatta ctgtttatgg
     2161 atgtgagcag gatgaggcag atgcattcca tgctctttcg cctcgctttg gcgttatggc
     2221 aacgataatt aacgccaacg tgtcggaatc caacgccaaa tccgcgcctt tcaatcaatg
     2281 tatcagtgtg ggacataaat cagagatttc cgcctctatt cttcttgcgc tgaagagagc
     2341 cggtgtgaaa tatatttcta cccgaagcat cggctgcaat catatagata caactgctgc
     2401 taagagaatg ggcatcactg tcgacaatgt ggcgtactcg ccggatagcg ttgccgatta
     2461 tactatgatg ctaattctta tggcagtacg caacgtaaaa tcgattgtgc gctctgtgga
     2521 aaaacatgat ttcaggttgg acagcgaccg tggcaaggta ctcagcgaca tgacagttgg
     2581 tgtggtggga acgggccaga taggcaaagc ggttattgag cggctgcgag gatttggatg
     2641 taaagtgttg gcttatagtc gcagccgaag tatagaggta aactatgtac cgtttgatga
     2701 gttgctgcaa aatagcgata tcgttacgct tcatgtgccg ctcaatacgg atacgcacta
     2761 tattatcagc cacgaacaaa tacagagaat gaagcaagga gcatttctta tcaatactgg
     2821 gcgcggtcca cttgtagata cctatgagtt ggttaaagca ttagaaaacg ggaaactggg
     2881 cggtgccgca ttggatgtat tggaaggaga ggaagagttt ttctactctg attgcaccca
     2941 aaaaccaatt gataatcaat ttttacttaa acttcaaaga atgcctaacg tgataatcac
     3001 accgcatacg gcctattata ccgagcaagc gttgcgtgat accgttgaaa aaaccattaa
     3061 aaactgtttg gattttgaaa ggagacagga gcatgaatag aataaaagtt gcaatactgt
     3121 ttgggggttg ctcagaggag catgacgtat cggtaaaatc tgcaatagag atagccgcta
     3181 acattaataa agaaaaatac gagccgttat acattggaat tacgaaatct ggtgtatgga
     3241 aaatgtgcga aaaaccttgc gcggaatggg aaaacgacaa ttgctattca gctgtactct
     3301 cgccggataa aaaaatgcac ggattacttg ttaaaaagaa ccatgaatat gaaatcaacc
     3361 atgttgatgt agcattttca gctttgcatg gcaagtcagg tgaagatgga tccatacaag
     3421 gtctgtttga attgtccggt atcccttttg taggctgcga tattcaaagc tcagcaattt
     3481 gtatggacaa atcgttgaca tacatcgttg cgaaaaatgc tgggatagct actcccgcct
     3541 tttgggttat taataaagat gataggccgg tggcagctac gtttacctat cctgtttttg
     3601 ttaagccggc gcgttcaggc tcatccttcg gtgtgaaaaa agtcaatagc gcggacgaat
     3661 tggactacgc aattgaatcg gcaagacaat atgacagcaa aatcttaatt gagcaggctg
     3721 tttcgggctg tgaggtcggt tgtgcggtat tgggaaacag tgccgcgtta gttgttggcg
     3781 aggtggacca aatcaggctg cagtacggaa tctttcgtat tcatcaggaa gtcgagccgg
     3841 aaaaaggctc tgaaaacgca gttataaccg ttcccgcaga cctttcagca gaggagcgag
     3901 gacggataca ggaaacggca aaaaaaatat ataaagcgct cggctgtaga ggtctagccc
     3961 gtgtggatat gtttttacaa gataacggcc gcattgtact gaacgaagtc aatactctgc
     4021 ccggtttcac gtcatacagt cgttatcccc gtatgatggc cgctgcaggt attgcacttc
     4081 ccgaactgat tgaccgcttg atcgtattag cgttaaaggg gtgataagca tggaaatagg
     4141 atttactttt ttagatgaaa tagtacacgg tgttcgttgg gacgctaaat atgccacttg
     4201 ggataatttc accggaaaac cggttgacgg ttatgaagta aatcgcattg tagggacata
     4261 cgagttggct gaatcgcttt tgaaggcaaa agaactggct gctacccaag ggtacggatt
     4321 gcttctatgg gacggttacc gtcctaagcg tgctgtaaac tgttttatgc aatgggctgc
     4381 acagccggaa aataacctga caaaggaaag ttattatccc aatattgacc gaactgagat
     4441 gatttcaaaa ggatacgtgg cttcaaaatc aagccatagc cgcggcagtg ccattgatct
     4501 tacgctttat cgattagaca cgggtgagct tgtaccaatg gggagccgat ttgattttat
     4561 ggatgaacgc tctcatcatg cggcaaatgg aatatcatgc aatgaagcgc aaaatcgcag
     4621 acgtttgcgc tccatcatgg aaaacagtgg gtttgaagca tatagcctcg aatggtggca
     4681 ctatgtatta agagacgaac cataccccaa tagctatttt gatttccccg ttaaataaac
     4741 ttttaaccgt tgcacggaca aacggttctg ttgcaaagtt ttaaataaag aataaaatcc
     4801 ct
//
LOCUS       DAZKNH010000286         4676 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.guided.139, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000286 DAZKNH010000000
VERSION     DAZKNH010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.guided.139"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            166..663
                     /gene="dfrG"
                     /locus_tag="ACG8MG_002860"
     CDS             166..663
                     /gene="dfrG"
                     /locus_tag="ACG8MG_002860"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0376798.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(735..2687)
                     /locus_tag="ACG8MG_002861"
     CDS             complement(735..2687)
                     /locus_tag="ACG8MG_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0376799.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2694..2930)
                     /locus_tag="ACG8MG_002862"
     CDS             complement(2694..2930)
                     /locus_tag="ACG8MG_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376800.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3332..3622
                     /locus_tag="ACG8MG_002863"
     CDS             3332..3622
                     /locus_tag="ACG8MG_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376801.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(3894..4243)
                     /locus_tag="ACG8MG_002864"
                     /pseudo
     CDS             complement(3894..4243)
                     /locus_tag="ACG8MG_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            4336..4638
                     /locus_tag="ACG8MG_002865"
                     /pseudo
     CDS             4336..4638
                     /locus_tag="ACG8MG_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
BASE COUNT         1495 a          758 c          821 g         1602 t
ORIGIN      
        1 aattgaaaat gataattgaa tgaaaatttg tgattttgtg gtattatgat aatataactt
       61 atagaaaaaa ttgcttattc aactaacggg caggataatg agaaaaggtt ttttgataga
      121 aataagaaat tagatggtaa attagttaaa aaggctgtgt tataaatgaa agtttctttg
      181 attgctgcga tggataagaa tagagtgatt ggcaaagaga atgacattcc ttggaggatt
      241 cccaaggact gggaatatgt taaaaatact acaaagggac atccgataat attaggtagg
      301 aagaaccttg aatcaatcgg aagagcctta cctgacagaa gaaatattat tctgacgaga
      361 gataaggggt ttacctttaa tggttgtgaa attgttcatt caatagaaga tgtttttgag
      421 ttatgtaaaa acgaagaaga aatttttatt ttcggaggag aacagattta taatttgttt
      481 ttcccttatg ttgagaaaat gtacatcaca aaaatacatc atgaattcga aggagatact
      541 ttttttccag aagtgaatta tgaggaatgg aatgaggtat ttgcccaaaa agggataaag
      601 aatgataaaa atccgtataa ctactatttt catgtatatg aaagaaaaaa cttattgagt
      661 taacgggtgc gttaattgaa gaagaacagt aaagataaag cacaagagct tgggattact
      721 ccaagctctt tttgtcattt gaaataaatt atatctttcc aatcaaattg tttatctgta
      781 atccctaatc tttgagcagg tgtcaaaacc tttttatccg ctgatttaaa agggcgacaa
      841 aagttgtagt aagttcttag aattgtaatc gcaaattgag catacttcgg attgaagtta
      901 gcgtaaatat agctcttctt atcaccacga gctgttgtta atggtctttc aagagatgat
      961 atataacgtc ttatctgttg aataaagcta ttggtcgaat ggtcattaac atttaatagc
     1021 atctttgcga tttcgtttgg ttccagagca gataaatccg ttctacaatc aactgaataa
     1081 aatcctttat ccttagtggc taatggatgt ttgatagggt tatcggcata tttgcgatat
     1141 ctttctccat ctttactaat agcctcttcg tggaattgat gacttgttag taattcagtt
     1201 agataatgag atgccaaagt ccgtagaaac tttgttttga atcctctgat atctccccaa
     1261 tcgagcagtt ctgcttttgc ttgtccgaat tcttttaagc attgttttct gcttttcgtc
     1321 ttgttaactt ggcaaataaa atgatgtgca tcgctcaacc ttaattcttt cgtgaatact
     1381 ctataaaagg cgtttctaat agagaagtca tcatcactaa tcattcgcca ttcgtcggca
     1441 ttaaccattt gtttaattaa ccaatagtga gcagttgtag tataaggagc atcaacattt
     1501 aaaccatcga caaatctact cctattagta attttcccta attcgtgaag ataagcattc
     1561 ctattttcgc tatcgttagc ggatggttct tgtggataat aagaccaatc taacctatca
     1621 tttttacgac taaaagtatt gagatggtct tctttgaatt ttcttgtatc ttcatttaat
     1681 tcatccattg aaatattcca atcataggca acatcagaac ggaacacata tcttgataag
     1741 acctcagcag atacaactat atacgtttgc atattaaggt cttcaaaccc cgtatatttc
     1801 tttgaacctt gtcctttctt acggacgtta tttaggtagt agtgcatctt gtccgtatta
     1861 agccacatct cgttaaattt ctttgtttga aggggttgtg tctcatatcg ttctaaaaac
     1921 tccaaacaac gcctatacag ccactccagc ttatgatagt aggttccaac tccaattcct
     1981 aaaatatcac aagtacgact gactgaaacc cttccaacaa ccatcttagc gaacattgga
     2041 agaatggtgt tcttttgttg gtgataagta gtggtttctt ctctttttgg tagaacatta
     2101 gtgaacttct tacacgcctt acattgatag cgttgagatt tgcccctact tttccccctt
     2161 ttgtaaaatt gcttcggctc attgaatggg gttgattctt cttccgagca gccttcttta
     2221 tgaaattgat aatctggctc aacatcttgg atgctattga tttctataag gcgtttaatt
     2281 tcttcaacaa cagaccaatt agataaaggg gttactgtac agttttgcga tacacctctg
     2341 ccaataggat tgtcattgca gtacatacct ttatgacctt tatctttgga actaccagtc
     2401 attttatatc gacttggttt cccttttaca tctttgaatt tgtgctgttc tttcccaaaa
     2461 ttacaacaaa acggattgat gcagtaatta tattgaatgg gatattcaga accgttccat
     2521 ttaaaagacg ttggcaagaa caacattttg ttataccttg ttgcaaatcg ttttggagtt
     2581 aataaatcat attgtttagc cctatctttt aattcttcgt ttgaaatcgg aattttaaca
     2641 tctacaattt catcttcctt cgtggctaat cttttaagct tggtcattgt ctatcaaccc
     2701 actctatctt tgatacccat tttttcaaca atccgcttat tacttcgctt gttggcaatc
     2761 cattcaataa acttcttatc gtctaaaatg ttcaataaga attcatccac tgcttcgctt
     2821 tcagtacgct ctgcatattc agcgtattgc ttaactatca cccttacttg ctctgatatt
     2881 tcccaatcca cagacttagc gttcttattt tttggctcaa gaaattgcat aggaaaatgc
     2941 accccttttt gcttagtaaa ttgtaaccat tttaacacac caatttgcat tcctacaata
     3001 attatttcac gttcgcattt ttatatacat tttaacttac attttgtggt gtaaattaac
     3061 aacaaacgtt gatttaacaa taaaaaacga gtatcaaatt cagttgcaat ttgatactca
     3121 tttaggttta cattttcact ttttgtactc caaaactgaa ctacttaaat ctatactgct
     3181 gcctcttttt tttagtacat gtccttcatc ttcgcaggaa gtgccaatat gattttaaga
     3241 aaggtaatag acaaaaagtc aaaaagataa aacagagttt cttctttaca aagggaatgt
     3301 atgttcgtat aatggtattg aggtgaaggt tatggagaaa aaatatggaa cagtagataa
     3361 gatccgtact ttgcaagtta gtgccagacc gttgatccgt ttcagtttaa atggggtcaa
     3421 ttgtttgatt gcttcccata gtttgaactt cctagctgaa gtagacgaag ggatgaagtt
     3481 agtcgttaca ggttattata ataatagaaa gcaatttgtt gtgagatcct atcatgtgtt
     3541 agggaaaccc aaaattgtgg tagaatatga gaaaagtcta tacccaagaa aaaaggtccg
     3601 acaagatgaa agaataaact agaaaacaag cggctgagac aaaaatgtcc tagccgcttg
     3661 ttttggaaag atggatgcag acgatgggat ttgaacccac acgagcatac gctcacaaga
     3721 acctcaatct atatataact acatgtattt aagtatttgt gatgaatcac taaactttcc
     3781 aaactcattt gagggatatt gcttaaagat tgaacagatc aattcgaagc cctattagta
     3841 ttcgctcaat ggggggatca gaggccataa aaagagacta gtcattacta gtctcaggta
     3901 gtggaattgt aggattttaa acgttcctgg agagtaccgg tatgtaactc gaagaggtga
     3961 ttatcatagt cgtaaaagta aattgaagaa gcctcgcctt tcacacgaga acgtcctggt
     4021 tcgacagtta aacctaacat ttggattttt gagacaaacg aatctatatc ttgttcgtca
     4081 attttaaaag caatatgatt gtatgtcttt ggcagtttat tcgacgaatt ctgcattact
     4141 gcaatccata ggtccttaac taagaaaaat ttttctggat ataatgaaaa tttttttgtt
     4201 gactatcgta gatttctttt gcatcaaata aggtatttaa caattaagta tttaaaagat
     4261 aacttttaag ttatctttaa gtaatgctta aagctatgtt atactctatt aggaaatata
     4321 agaaggagta taaaaatgaa caaacataat agaaaaataa cgaataacaa agaactgatc
     4381 ttgttactaa tagggatttg gggtatcttt ttactcttta atatgtatat gccgactatg
     4441 agagcggatg acgtagttta tgcaagtaga ttagatgaac ttggctattt gggtgcttcg
     4501 atagaacatt ataagacatg gagttcaaga attattatag aactattctt aatgttcttt
     4561 tctaaacact ttatgctatg gaaattatta aattctacga ttatgttagg aattgtagta
     4621 ttactttgca agtatgttga gagtataaaa tattttgtgt aaatgaaaaa atccat
//
LOCUS       DAZKNH010000287         2695 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.guided.172, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000287 DAZKNH010000000
VERSION     DAZKNH010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.guided.172"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            260..1171
                     /locus_tag="ACG8MG_002866"
     CDS             260..1171
                     /locus_tag="ACG8MG_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0376802.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1324..1809
                     /gene="vanZ-A"
                     /locus_tag="ACG8MG_002867"
     CDS             1324..1809
                     /gene="vanZ-A"
                     /locus_tag="ACG8MG_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0376803.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2328..2612
                     /locus_tag="ACG8MG_002868"
     CDS             2328..2612
                     /locus_tag="ACG8MG_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0376804.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
BASE COUNT          995 a          392 c          502 g          806 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt caaggaggta aggatggcgg aatgaaacca acgaaattaa
     1201 tgaacagcat tattgtacta gcacttttgg ggtaacgtta gctttttaat ttaaaaccca
     1261 cgttaactag gacattgcta tactaatgat acaacttaaa caaaagaatt agaggaaatt
     1321 atattgggaa aaatattatc tagaggattg ctagctttat atttagtgac actaatctgg
     1381 ttagtgttat tcaaattaca atacaatatt ttatcagtat ttaattatca tcaaagaagt
     1441 cttaacttga ctccatttac tgctactggg aatttcagag agatgataga taatgttata
     1501 atctttattc catttggctt gcttttgaat gtcaatttta aagaaatcgg atttttacct
     1561 aagtttgctt ttgtactggt tttaagtctt acttttgaaa taattcaatt tatcttcgct
     1621 attggagcga cagacataac agatgtaatt acaaatactg ttggaggctt tcttggactg
     1681 aaattatatg gtttaagcaa taagcatatg aatcaaaaaa aattagacag agttattatt
     1741 tttgtaggta tacttttgct cgtattattg ctcgtttacc gtacccattt aagaataaat
     1801 tacgtgtaag atgtctaaat caagcaatct gatctttcat acacataaag atattgaatg
     1861 aattggatta gatggaaaac gggatgtggg gaaactcgcc cgtaggtgtg aagtgagggg
     1921 aaaaccggtg ataaagtaaa aagcttacct aacactatag taacaaagaa agcccaatta
     1981 tcaattttag tgctgaggaa ttggtctctt taataaattt ccttaacgtt gtaaatccgc
     2041 attttcctga cggtaccccc actagcagcc atgctttttt tgaatctttg taggacgttt
     2101 taaagagaat tacccacttt aaaattaaac atgcgcttag aatcgcttta ggaaacacga
     2161 tccagtccaa taatcgtcga taaaaacttt tgaaaaaggt tggtgaaatt acctactttt
     2221 ggaataatca caaatcacaa gtgattaatc acttgtttat taagatatta aaagctataa
     2281 tttaaataaa gcgtgaattt tattacacaa aaagaggggg gagaaacttg gaactagcat
     2341 ttagagaaag cttaaaaaag atgagaggta ccaaatcaaa agaaaaattc tcccaagaat
     2401 tagaaatgag tagatcaaat tattcacgaa tagaatcagg aaaatcagat ccaaccataa
     2461 aaacactaga acaaattgca aagttaacta actcaacgct agtagtggat ttaatcccaa
     2521 atgagccaac agaaccagaa ccagaaacag aatcagaaca agtaacattg gatttagaaa
     2581 tggaagaaga aaaaagcaat gacttcgtgt gaataatgca cgaaatcgtt gcttattttt
     2641 tttaaaagcg gtatactaga tataacgaaa caacgaactg aatagaaacg aaaaa
//
LOCUS       DAZKNH010000288         1319 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.guided.209, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000288 DAZKNH010000000
VERSION     DAZKNH010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.guided.209"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..769
                     /locus_tag="ACG8MG_002869"
     CDS             <1..769
                     /locus_tag="ACG8MG_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HFQ0376805.1"
                     /translation="FVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLM
                     LFDDYNKIDLTLLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSARE
                     YDDCCNEFWNVTPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVG
                     KNYKFIERYISEDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPE
                     YDRNITKYTRDMYKKYTGKTGCLDSTYAADIEERREQ"
     gene            766..1246
                     /locus_tag="ACG8MG_002870"
                     /pseudo
     CDS             766..1246
                     /locus_tag="ACG8MG_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          455 a          191 c          293 g          380 t
ORIGIN      
        1 ttttgtaagt gatatagaac cgtttatatc taatgatgac tggcttaatc aatttgggaa
       61 tataataatg atgcaaaagc cggaggatat ggaattattc ccacctgaag aaaagggatt
      121 ttcctatctt atgctatttg atgattacaa taaaattgat cttaccttat tgcccttgga
      181 agagttagat aattacctaa agggcgataa attaataaag gttctaattg ataaagattg
      241 tagaattaaa agggacatag ttccgactga tatagattat catgtaagaa agccaagcgc
      301 aagggagtat gatgattgct gcaatgaatt ttggaatgta acaccttatg ttattaaagg
      361 attgtgccgt aaggaaattt tatttgctat tgatcatttt aatcagattg ttcgccatga
      421 gctgctgaga atgatatcat ggaaggtcgg catcgaaaca ggctttaaat taagtgtagg
      481 caagaactat aagtttattg aaaggtatat atccgaggat ttgtgggaga aacttttgtc
      541 cacctaccgg atggattcct atgaaaacat atgggaagca ttatttctat gccatcaatt
      601 gttcagggcg gtatccggtg aggtggcgga aaggcttcat tatgcctatc cggagtatga
      661 taggaatata acaaaatata ccagggacat gtataaaaaa tacactggta aaaccggctg
      721 cctggatagc acatatgccg ctgatataga agagaggcgg gaacagtgat tacagaaatg
      781 aaagcagggc acctgaaaga tatcgataaa cccagcgaac catttgaggt gataggtaag
      841 attataccga ggtatgaaaa cgagaattgg acctttacag aattactcta tgaagcgcca
      901 tatttaaaaa gctaccaaga cgaagaggat gaagaggatg aggaggcaga ttgccttgaa
      961 tatattgaca atactgataa gataatatat cttttatata gaagatatcg ccgtatgtaa
     1021 ggatttcagg gggcaaggca taggcagcgc gcttatcaat atatctatag aatgggcaaa
     1081 gcataaaaac ttgcatggac taatgcttga aacccaggac aataacctta tagcttgtaa
     1141 attctatcat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt
     1201 tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag
     1261 tgaattggag ttcgtcttgt ggttctgttg caaagtttta aataaagaat aaaatccct
//
LOCUS       DAZKNH010000289         1168 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.guided.221, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000289 DAZKNH010000000
VERSION     DAZKNH010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.guided.221"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..601
                     /gene="catA"
                     /locus_tag="ACG8MG_002871"
     CDS             <1..601
                     /gene="catA"
                     /locus_tag="ACG8MG_002871"
                     /EC_number="2.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type A chloramphenicol O-acetyltransferase"
                     /protein_id="HFQ0376806.1"
                     /translation="FEHYFNQQTTYSITKEIDITLFKDMIKKKGYEIYPSLIYAIMEV
                     VNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWTESDKNFISFYNNYKND
                     LLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNSSFLLPIITIGKFYSEN
                     NKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
BASE COUNT          416 a          136 c          187 g          429 t
ORIGIN      
        1 ttttgaacac tattttaatc agcaaactac ttatagcatt actaaagaaa ttgatattac
       61 tttgtttaaa gatatgataa aaaagaaagg atatgaaatt tatccctctt taatttatgc
      121 aattatggaa gttgtaaata aaaataaagt gtttagaaca ggaattaata gtgagaataa
      181 attaggttat tgggataagt taaatccttt gtatacagtt tttaataagc aaactgaaaa
      241 atttactaac atttggactg aatctgataa aaacttcatt tctttttata ataattataa
      301 aaatgacttg cttgaatata aagataaaga agaaatgttt cctaaaaaac cgatacctga
      361 aaacaccata ccgatttcaa tgattccttg gattgatttt agttcattta atttaaatat
      421 tggtaacaat agcagctttt tattgcctat tattacgata ggtaaatttt atagtgagaa
      481 taataaaatt tatataccag ttgctctgca acttcatcat tctgtatgtg atggttacca
      541 tgcttcacta tttatgaatg aatttcaaga tataattcat agggtagatg attggattta
      601 gtttttagat tttgaaagtg aatttaattt tatacacgta agtgatcata aaatttatga
      661 acgtataaca accacatttt ttggttgctt gtggttttga ttttgaattt ggttttgaac
      721 ttatggactg atttattcag tccatttttt gtgcttgcac aaaaactagc ctcgcagagc
      781 acacgcatta atgacttatg aaacgtagta aataagtcta gtgtgttata ctttacttgg
      841 aagatgcacc gaataaaaaa tattgaagaa caactagcaa aagattttaa agagttattt
      901 tattttaagt ctttataaca tgagtgaagc gaatttttaa atttcgatag aaatttttac
      961 atcaaaaagc cccctgtcaa aattgacgaa gggggttttt tggcgcacgc ttttcgttag
     1021 aaatatacaa gattgaaaat cgtgtataag tgcgcccttt gttttgaact tagcacgtta
     1081 catcaatttt ttaaaatgat gtataagtgc gcccttttaa attttgagtg attatatttt
     1141 ttgagttaga aaaagggatt gggaaaat
//
LOCUS       DAZKNH010000290         1085 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.guided.229, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000290 DAZKNH010000000
VERSION     DAZKNH010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.guided.229"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..76
                     /locus_tag="ACG8MG_002872"
     CDS             26..76
                     /locus_tag="ACG8MG_002872"
                     /inference="COORDINATES: protein motif:HMM:NF018061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFQ0376807.1"
                     /translation="MWTLSKQTKNSDYADK"
     gene            201..938
                     /gene="erm(B)"
                     /locus_tag="ACG8MG_002873"
     CDS             201..938
                     /gene="erm(B)"
                     /locus_tag="ACG8MG_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0376808.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          404 a          184 c          171 g          326 t
ORIGIN      
        1 atggcctttt atcttttaca caattatatg gactttatcg aaacagacta aaaacagtga
       61 ttacgcagat aaataaatac gttagattaa ttcctaccag tgactaatct tatgactttt
      121 taaacagata actaaaatta caaacaaatc gtttaacttc tgtatttgtt tatagatgta
      181 atcacttcag gagagattac atgaacaaaa atataaaata ttctcaaaac tttttaacga
      241 gtgaaaaagt actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt
      301 acgaaattgg aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg
      361 taacgtctat tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga
      421 atactcgtgt cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga
      481 ggtataaaat tgttgggaat attccttacc atttaagcac acaaattatt aaaaaagtgg
      541 tttttgaaag ccgtgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta
      601 ccttggatat tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat
      661 tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa
      721 aacttacccg ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg
      781 tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag
      841 caatgaaaca cgccaaagta aacaatttaa gtaccattac ttatgagcaa gtattgtcta
      901 tttttaatag ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttttaaa
      961 tttggaaagt tacacgttac taaagggaat ggagataaat tattagatat actactgaca
     1021 gcttccaaga agctaaagag gtccctagcg cctacgggga atttgtatcg ataaggggta
     1081 caaat
//
LOCUS       DAZKNH010000291          857 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3534
            SAMEA115706779-rid22570033.guided.242, whole genome shotgun
            sequence.
ACCESSION   DAZKNH010000291 DAZKNH010000000
VERSION     DAZKNH010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706779
            Sequence Read Archive: ERR13196513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287777.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287777.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:34:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,874
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,727
            CDSs (with protein)               :: 2,727
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 41 of 87
            Pseudo Genes (incomplete)         :: 42 of 87
            Pseudo Genes (internal stop)      :: 23 of 87
            Pseudo Genes (multiple problems)  :: 16 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706779-rid22570033.guided.242"
                     /isolate="VRE3534"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..689
                     /gene="aac(6')"
                     /locus_tag="ACG8MG_002874"
     CDS             141..689
                     /gene="aac(6')"
                     /locus_tag="ACG8MG_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0376809.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          307 a          127 c          189 g          234 t
ORIGIN      
        1 ctgtataatt tttttatttt ataagtaaga taaataatga aagaatagag atactgattg
       61 gtagctaaag atttttgagc gagaacattt cgttttttca aaaggactgt tagaataagc
      121 aagaacaagg gggaagacgt atgataatca gtgaatttga ccgtaataat ccagtattga
      181 aagatcagct ttctgattta ctgagactga cttggccgga agaatatgga gacagctcgg
      241 cagaagaagt agaagaaatg atgaatccag aacgaatcgc ggtagcagcg gtagaccaag
      301 atgagttagt aggatttatt ggtgcaatcc ctcaatacgg tatcacaggt tgggaattgc
      361 atccattagt tgtagaaagc tcccgacgaa agaaccaaat aggtactcga ttagtcaatt
      421 acttagaaaa agaagtagct tccagaggag gaatcacgat ttatttaggt acggatgatt
      481 tagaccatgg aacaacgtta agtcaaacgg acctgtatga gcatacattt gataaagtgg
      541 cttccatcca gaaccttcgt gaacatccgt atgaattcta tgaaaaatta ggttataaaa
      601 tcgtaggtgt cttaccaaat gcaaatggct gggacaaacc ggatatttgg atggcaaaaa
      661 cgattattcc tcgaccaaat tctcaataat aaaaagaggt tgtgacagaa gtaatcagct
      721 tcaagaaata agaaggaatt tacgaaaatt acttcttgaa tttttgtgaa attccaactt
      781 atttccgaag aagctacttt tggaacaccg tgtaccagag gttgtgaaaa agtcgcttag
      841 tttcgagtag taagaaa
//
